LOCUS       DAOMFA010000001        32375 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_42_length_32375_cov_5.282488, whole genome shotgun sequence.
ACCESSION   DAOMFA010000001 DAOMFA010000000
VERSION     DAOMFA010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 32375)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32375)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32375
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42_length_32375_cov_5.282488"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..537)
                     /locus_tag="PKW44_00005"
     CDS             complement(<1..537)
                     /locus_tag="PKW44_00005"
                     /inference="COORDINATES: protein motif:HMM:NF013543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HQR50001.1"
                     /translation="MEGLTINLWILALLILVGLAFDFMNGFHDGANSISTIVATGVLT
                     PKQAVLFAAFFNFAALWLFHLKVAATIGKGTVDPSFVDYYVIFGALCGALSWNIITWL
                     YGIPSSSSHALVGGLVGATVAKAGSIAPIIWSGFGKILLFIIVSPLVGFLLGGLLMVL
                     VAWLFRNHPYGRPTGGDET"
     gene            complement(537..1163)
                     /locus_tag="PKW44_00010"
     CDS             complement(537..1163)
                     /locus_tag="PKW44_00010"
                     /inference="COORDINATES: protein motif:HMM:NF013982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HQR50002.1"
                     /translation="MPQRREFFDLLAAHSDRVVASANATLRLVNTLGPNNRDDIALLV
                     KEVDFNEKSGDKIKADVIALLHKSFITPFSRDEIHTLTLELDKVLGALQSVANAVGMY
                     NIQESTPEARELAALSADSCLRLNRAVIALGDKNRRKEAVALCMEIDDIETKADKVLK
                     KAITRLFQDDSNVWAAMKLREFYVLQENVLDYCEEAAKTIEQILIENS"
     gene            1409..1864
                     /locus_tag="PKW44_00015"
     CDS             1409..1864
                     /locus_tag="PKW44_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR50003.1"
                     /translation="MKISNPEYLAKVELLTADERERLLSRMAGKLPRKLERDKLSENE
                     ALAIQMALEDEQLQEWREKMHSLKAQAKGKEKADKAKKGAEKPAKAKGATGKAGEAPG
                     KPGTKMVAKKPVVKAPVARVATKPVAKPAVKPAAKTAAKPTAKTTKAAS"
     gene            complement(1988..2767)
                     /gene="pstB"
                     /locus_tag="PKW44_00020"
     CDS             complement(1988..2767)
                     /gene="pstB"
                     /locus_tag="PKW44_00020"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HQR50004.1"
                     /translation="MQTNNTPLKAEARGLDFYYNGGHKALKGINLPIYEKKITALIGP
                     SGCGKSTFLRCFNRMHDLYPGNVYDGAIVMHPDNTNILAKGVDPIEVRMRISMVFQKP
                     NPFPKSIYENVAYGLRVRGETNKREMDDKVEEALRGAAIWGEVKDRLNEVAFNLSGGQ
                     QQRLCIARALATDPEIMLFDEPTSALDPIATASIEELMSELKEKVTILVVTHNMQQAA
                     RISDYTAYMYLGELIEFDDTDLMFTKPSRKETEDYITGKFG"
     gene            complement(2780..3721)
                     /gene="pstA"
                     /locus_tag="PKW44_00025"
     CDS             complement(2780..3721)
                     /gene="pstA"
                     /locus_tag="PKW44_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HQR50005.1"
                     /translation="MSGQQASTLKDLDAVRAMIRHHKLNDTIFALIGLLALMVGLLTL
                     LTLFIDLLMDGYTRFNAGLFTEFPSRHAAKAGLLSAWVGSLLVMSVTLVTAVPLGVAA
                     GVYLEEYAPKNWFSDVIEINVTNLAGVPSIIYGLLALGLFVYDLGLGQSIQTAGLTLA
                     LLILPVVIVNTRESIRAIPVHIREAAYAVGATKWQMVSDHILHYSFGGILTGIIIGMA
                     RALGETAPVITIGALTYIAFLPPSPIQPAPPYLNFEWLSSGFTVLPIQMFNWLSRPDH
                     AFHVNAAATGVVIIVVTLLMNGTAIWLRYRIRKNIKW"
     gene            complement(3724..4665)
                     /gene="pstC"
                     /locus_tag="PKW44_00030"
     CDS             complement(3724..4665)
                     /gene="pstC"
                     /locus_tag="PKW44_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HQR50006.1"
                     /translation="MATQVLHNPLEHVISDRLARNFRRHFKERLIEFILMLAALSAVA
                     TTVAIVCILLYESSTFFKTVSLVEFFTDTQWTPLFEDAHYGILPLISGTVTTSAIALL
                     VAVPLGTIGAIYLSEFASHKVREICKPILELLVGVPTVVFGYFALLFVTPLLQKIIPG
                     LPSFNMLGPGIVMGIMIIPYISSVAEDAMRAVPMSMREGSYAMGATRFQTAVRVVAPA
                     ATSGIIAAYILGISRAVGETMVVAIAAGQQPNLTFNPMDGAATITAYIVQVAMGDLPH
                     GSIGYQSIFAAGLMLMLITLFLNLLGHLARKKYREAY"
     gene            complement(4753..5760)
                     /locus_tag="PKW44_00035"
     CDS             complement(4753..5760)
                     /locus_tag="PKW44_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HQR50007.1"
                     /translation="MQFKFTQTLGIAAVVSGLFSTPATVLAAEKIVKIDGSSTVYPIT
                     EAVAEEFQNSKKGAIKVTVGISGTGGGFKKFCRGETDISEASRPILKKEMDACKEAGV
                     QYIELPVAYDALTVVINPKNDWIKSMTVDELKKMWAPGAQGSVKSWKQINAAWPDKPL
                     KLFGPGADSGTFDYFTEAIVGKAKSSRGDFTASEDDNVLVQGVSGDIGGLGYFGYAYY
                     EANQGKLKAVPIVAKAGAPAVEPSAETVMNGTYQPLSRPLFIYVNATAAAFKPEVKEF
                     INFYLANAPTLVKEVKYIPLPANEYKAVDAHWKLLKPGTGFGGVPEVGVKIEELLKRI
                     Q"
     gene            5999..7963
                     /locus_tag="PKW44_00040"
     CDS             5999..7963
                     /locus_tag="PKW44_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HQR50008.1"
                     /translation="MAKNKLSEDFAAYSQWRGVLMDGICDYRSWLNEQELNDAQIDLR
                     IQHLLDRLREDKLNVAFVAEFSRGKSELINAIFFADYGQRLLPSSAGRTTMCPTEFLY
                     DATKQTSLQLLPIETRSSDATTTEYKRYPDEWTVFQFDVASNESMIEAFKEVSRTKRV
                     SVEEAERYGLFDPDSNDDAMALNEDGSVDIPCWRYAIINFPHPLLEQGLVILDTPGLN
                     AIGTEPELTLNMLPNAHAVLFILSADTGVTKSEIDVWRHYISGTRWKQKARMAVLNKI
                     DGLWDELKDEQQIEAEINKQVTGSSELLGLNVNQIFPVSAQKGLLGKISGDDQLLARS
                     RLPELERALSDELIPSKKEIVRDNTQSELDDLLNNSQLILDARLKGIIEQLDELKGLR
                     GKNEDVVNHMMNKVKNDKENFERGLQRFQALRSVFSQQANALFTNLGMEALRSDVGET
                     RNAMVSANFTKNIRAAMDDFFFRLKERLVKSDEQVGEIKIMMDAMYQKFSQEHGLRHV
                     TPPSFSMLRDQKGVAKLERTYREQFNTTFKMLTNEKLTLTSRFFETLASRVINIYEVA
                     NQDTENWLKAVIAPMESQVREHQLQLRRRLESIKRIYKATDTLEDRIAEMESVEQSIH
                     RQIADLRELQHQINQALSFDRQEEVGVKAA"
     gene            7960..8169
                     /gene="ccoS"
                     /locus_tag="PKW44_00045"
     CDS             7960..8169
                     /gene="ccoS"
                     /locus_tag="PKW44_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HQR50009.1"
                     /translation="MTPILLFLFALTLLFVRLSAVGVLWAIKSGQFDDLEGPAQRILM
                     DDDDPMIPFKSLKSESTRHKIGVRQ"
     gene            complement(8359..9216)
                     /gene="rpoH"
                     /locus_tag="PKW44_00050"
     CDS             complement(8359..9216)
                     /gene="rpoH"
                     /locus_tag="PKW44_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HQR50010.1"
                     /translation="MSYSLSLPMPATADSLEHYIQSVRSFPFLSAEEEHDCATRLQRD
                     NDLDAAKQLILSHLRLVVSIARGYSGYGLPQADLIQEGNIGLMKAVRRFDPERGVRLV
                     SFAVHWIKAEIHEFILRNWRLVKVATTKAQRKLFFNLRSMKQHSGSLHSDEIAEIARE
                     LNVKPEDVAEMESRMGGHDIALESHGDEDGEEGWSPIAFLEDQRPEPSEFLAEKQIGV
                     LHASGLEMALNSLDERSRHIVEARWLREDNAATLHELAAEYGVSAERIRQIEQKALQK
                     MKGVIGLAA"
     gene            complement(9294..10205)
                     /gene="ftsX"
                     /locus_tag="PKW44_00055"
     CDS             complement(9294..10205)
                     /gene="ftsX"
                     /locus_tag="PKW44_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HQR50011.1"
                     /translation="MISQWLSQHRQSFRQVLCRMWRNRLATLMMLGVMGATLSLPALL
                     YVAVDNLSRFSGDVRSEPQISLFVALDAKPEAIQDIGDRLRRQHGIRSYHFVPRDEAW
                     QKLRQNAGLSDIADGLEQNPLPDAYIILPDNDTPEVIEQMQQEMQQWPEVAHAQLDTA
                     WVKRLYSLVELGKKAVAVLAGLLGFALLAVIGNTIRLQILTQRDEIEVSALIGATGRF
                     IRRPFLYAGALYGLGGGLVAWLFLWGVVDLFNLSVDDLARLYASDFRLQMPDWQAALA
                     LVGGAVGLGWLGAYWSASRALSDIDPL"
     gene            complement(10202..10852)
                     /gene="ftsE"
                     /locus_tag="PKW44_00060"
     CDS             complement(10202..10852)
                     /gene="ftsE"
                     /locus_tag="PKW44_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HQR50012.1"
                     /translation="MIRFDQVGKLYPGGFEALKKVSFEIDKGELVFITGHSGAGKSTL
                     LKLVAALERPTSGTVLIGQQNVSKLRPAAVPYLRRKFGMVFQDHKLLYDRNVFENVAL
                     PLSINGIVGQEAAKRVRAALDKVGLLDKEKAMPVTLSGGEQQRLAIARAVVSRPSILV
                     ADEPTGNLDETYAAEIMKLFQAFHQVGVTVLIATHDTHGAAASGGRVMHLDHGVLQ"
     gene            complement(10849..11811)
                     /gene="ftsY"
                     /locus_tag="PKW44_00065"
     CDS             complement(10849..11811)
                     /gene="ftsY"
                     /locus_tag="PKW44_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018508876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HQR50013.1"
                     /translation="MLNFFKKDPQPATAPEPTSPSWGERLKQGLARTRDNLGNSLGTL
                     FGGGKIDEAVYEELETILLTADVGVGATQALLEEVRKEVKRQAMSDTSQLRSALQEAL
                     IRLLEPLAQPLDAGSSRPYVIMLAGVNGAGKTTTIGKLAWLLQAQGKSVLIAAGDTFR
                     AAAREQLEAWGERNDVHVVSQSGGDPAAVIFDAVNSARARGIDVVLADTAGRLPTQLN
                     LMEEIRKVKRVIDKALPGAPHEVLLVLDANTGQNAVTQVKAFDDALQVTGLALTKLDG
                     TAKGGVIAAIAQARPIPIRFIGVGEQIEDLQPFNAREFVEALFT"
     gene            11916..12509
                     /locus_tag="PKW44_00070"
     CDS             11916..12509
                     /locus_tag="PKW44_00070"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HQR50014.1"
                     /translation="MIQAVGQIEHIEPAGNDVRLSIASGALDMGDVCVGDSIATSGVC
                     LTVITCDEKSFQVHVSRETLSCTVGLDTTKPVNLEKALRLADRLGGHLVSGHVDGVGQ
                     VVRFDPVSDCMLLCIRAPHALLRYIAVKGSIAVDGVSLTVNTVEHDAFSINLIPHTLE
                     MTTLRHLGVGSRVNLEVDPIARYVERMTQWANEGEPA"
     gene            12506..13597
                     /gene="ribBA"
                     /locus_tag="PKW44_00075"
     CDS             12506..13597
                     /gene="ribBA"
                     /locus_tag="PKW44_00075"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HQR50015.1"
                     /translation="MISAVTEIIDEIRHGRMVVLVDEEDRENEGDLVLAAQFVTPEHI
                     NFMARHGRGLICLTLTEPRCRQLNLPLMVRDNGARMGTNFTVSIEAAQGVTTGISAAD
                     RALTIQAAVARDARAADIVSPGHVFPLMAQNGGVLVRAGHTEAGCDLATLAGLAPAAV
                     ICEILKDDGSMARLPDLCEFAEQHSIKIGTIADLIRYRSQTERLVERAAERRVATPYG
                     AMRLVAYYDKTANETHLVLVKGEPGPEQEVLVRVHEPLSVFDLLDGDSQTHSWSVLDA
                     MKVIQQAPCGVMILLRRNEDGQQLVERIQHADEPVRSRQDLRDYGIGSQILLDLGVRK
                     MKLMAVPRKMPSMAGFGLEVTGYMEPGNS"
     gene            13611..14171
                     /locus_tag="PKW44_00080"
     CDS             13611..14171
                     /locus_tag="PKW44_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HQR50016.1"
                     /translation="MENVNLTGHFLIAMPAMADPYFARSVTYVCEHNANGAMGVVINR
                     PIDMNLDDLFGQIEVELIDKDLGQTPVHFGGPVQIDRGFVLHQPVGQWQSTIAVSRDT
                     GLTTSKDILEASAHGDGPKKMLVTLGYAGWAAGQLEEELGQNAWLTVPAQESVIFDVA
                     SEDKFSAAMGLLGLDFASLSEEAGHA"
     gene            14164..14601
                     /gene="ruvX"
                     /locus_tag="PKW44_00085"
     CDS             14164..14601
                     /gene="ruvX"
                     /locus_tag="PKW44_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HQR50017.1"
                     /translation="MPEAGTVLAFDFGERRIGVALGDFGLGIAHPLATIDTESNDARF
                     KAIAAMIGEWKPAQLVVGLPAYLDGAEHELSRLCRKFANRLHGRFGLPVALVDERLTS
                     AEASQSLREAGVGSRRQKPMLDQVAAQHILQSYFDAHTHAHAA"
     gene            14591..15085
                     /gene="pyrR"
                     /locus_tag="PKW44_00090"
     CDS             14591..15085
                     /gene="pyrR"
                     /locus_tag="PKW44_00090"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HQR50018.1"
                     /translation="MLPDAEFLVETLAEQIRPLLGQNAALVGIHSGGAWLAQRLQQQL
                     GGDIPLGTLDISFYRDDFSRIGLHSQVKPSSINFEVEGCHIILVDDVLYTGRTIRGAM
                     NELFDYGRPARITLAVLVDRGGREIPVEAQIVGARLELDPGENLELARDETGKFHFIL
                     HEAA"
     gene            15082..16038
                     /locus_tag="PKW44_00095"
     CDS             15082..16038
                     /locus_tag="PKW44_00095"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HQR50019.1"
                     /translation="MNNPQLTKDGKLKHLLSIEGLPRSILNQILDTAESFAGVSEREV
                     KKVPLLRGKTVCNIFFENSTRTRTTFEIAAKRLSADVVSLNVGTSSQAKGETILDTVD
                     NLIAMHADMFVVRHAQSGAAHFIARHVPPHIHVINAGDGRHSHPTQGLLDVFTIRRHK
                     REFHNLRIAIVGDVLHSRVARSEIHALTTLGVPEVRVIAPKTLLPTDVERLGVQVYHD
                     MREGLKDVDVVMMLRLQNERMSGALLPSAQEYFKCYGLTQEKLALAKPDAIVMHPGPM
                     NRGVEIDSSVADGSQSVILPQVTYGIAVRMAVMSILAGNSEQ"
     gene            16035..17312
                     /locus_tag="PKW44_00100"
     CDS             16035..17312
                     /locus_tag="PKW44_00100"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HQR50020.1"
                     /translation="MKIHIKNGRLIDPRNGVDAVTDLYIAAGKVVAVGRTPVDFSVNQ
                     TIDASGLVVCPGLVDLSARLREPGFEYKATLESELQAAVAGGVTSLACPPDTDPVLDE
                     PGLVQMLKHRAKLLNLAHVYPLGALTRQLEGKQLTEMCELHEAGCVGFAQADAPLTDT
                     RVLLRAMQYAATFGFTVWLRPEDAFLAQGGVAHDGEIAARLGLAGIPTVAETVALATI
                     LRLAAETGTKLHLCRLSTRAGIEMVRKAKQDGLAVTCDVAANHLQLTEHDIGFFDAHC
                     HLKPPLRTQRDLEALKRGLSDGTIDAVCSDHTPVDDDAKLLPFAEAEAGATGLELLLP
                     LTLKWAADTGVALPDALARVTCIPADILGLDAGHLGAGSDADICIFDPRQYWKVEAKS
                     LVSQGKNTPFLGLELAGRVRHTLVHGQVVYQPA"
     gene            17348..17761
                     /locus_tag="PKW44_00105"
     CDS             17348..17761
                     /locus_tag="PKW44_00105"
                     /inference="COORDINATES: protein motif:HMM:NF023696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3617 family protein"
                     /protein_id="HQR50021.1"
                     /translation="MQKILMACLLPLALADAWAGDVQPGLWRISLSMSAAGTDQVFGP
                     FEKTQCFTEADARDPGKLFADIGGTCSYGNKNYQGNHFSFTIQCGGALPMMGSGSVDY
                     SATSFQGELNLKANIPDTGNVETKSRVSGSRIGGC"
     gene            complement(17845..18738)
                     /locus_tag="PKW44_00110"
     CDS             complement(17845..18738)
                     /locus_tag="PKW44_00110"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HQR50022.1"
                     /translation="MSKFQVSAIIVNYNSGSHAVDCIRSLQAQQVDGLEIIVVDNASQ
                     DDSIALLRDACGGCITLIESRENLGFGRANNLAAVKAQGEFLLLINPDAQLQGDGAVT
                     TLVAYLQQNQAVGIAGPEIHEPSKNKYVLPRKTYPSQHRLKFTKGLAVLPGKIAWILG
                     ACLILRKSTFQQIHGFDPDFFLYGEDIDICLRVRKAGYAIGYCSAAKITHVGGASEAG
                     AIPLDKFLRKRRGFYLFCRKHYDARDVRRIARHAIASAKLGQLNLAIREILRSGSQQQ
                     RQQQAHKLQAARIAADEVLSE"
     gene            complement(18761..20641)
                     /locus_tag="PKW44_00115"
     CDS             complement(18761..20641)
                     /locus_tag="PKW44_00115"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HQR50023.1"
                     /translation="MRTHLTAALKNFALLSWLHMLTVTWAYFRMANPEIAFSLPLLFH
                     VLTWANYSLLYLLPGLLPAVLLARFWPARRMLTAAAAILGTSLCILFIRSDSVIYDLY
                     SFHFNGFVVNLLTTPGGIASLGGGNDTYVSIALIALGHALVQAALWKAGTWFASKRRS
                     FLRWHLVLALLVLAMVGERVIYGVSDIRNDGAILDTSRVYPFYGRTTFRGVASKFGVN
                     PQRQNKLSVTLDATRLNYPLQPVAYEMVVKPPNIVMLVAESLRWDRLTPEIMPNTWRL
                     AQRGQHFKHHYSSGNGTREGLFGMFYGLYGSYWSSFLYAQKSPLLMDRLQELGYQFDL
                     RTSAAFSYPEFNKTLFANIPLEQLHEEPANKEPWQRDQDNASALINFLQKRDPSRPFM
                     SFFFFESTHARYSFPDSAIIARPYLENVNYSTMTRASLAPRIDELLNRYTNSAHWLDV
                     QIGRIIDELEQKGLLDNTFFFFYGDHREEFLEKGFWGHNSSFVEQQILTPMVVWMPGM
                     QHSEIDRVTSHLDVGTTLMQILGAPKDASGYSLGRNLFDTGHRPYVVSSDWHSISVIT
                     DDIKYRIPYDTRGADHWSPTKPDDTPYSGQAASEALVRNHTLILDAIKDTSRFTRKAE
                     KK"
     gene            20837..22615
                     /gene="cptA"
                     /locus_tag="PKW44_00120"
     CDS             20837..22615
                     /gene="cptA"
                     /locus_tag="PKW44_00120"
                     /inference="COORDINATES: protein motif:HMM:NF007933.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HQR50024.1"
                     /translation="MAFANQANTTRNGIDWAGIGWLYLFFWYFSGLNHVLLQFTGTTI
                     FFGFRQSVFMSLLWLVPVLLFPHRTRAISAVIGVVLWFFSLFSLGYFCIYGQEFSQSV
                     IFIIFESNQAESSEYVAQYFSWWMIPALLVYSAVAVYLWKRIRPVFLPRGLAWAISLL
                     ILFFLFGYPLYRQAIVKQAPAERVREQMEDRMEPAVPWQMVIGYLHYREQLGNMQDLL
                     LTNAKVPPIANLVDANTGQPATLVLVIGESTNRQHMSLYGHPRPTTPKLDAMRDQLLV
                     FNNVFSPRPYTIETLQQVLTFADQKNPDWYLTRPSIMNMMKQAGYQTYWITNQQTMTK
                     RNTMLTTFSKQTDKQFYLNNSRAQNSRQYDDDVLEPFQQILREPAPRKFIVVHLLGTH
                     MKYEYRYPPEYERFTNRDGVPQALTDEQAAIYNTYDNAVLFNDHVVSSLIEDLAATKS
                     KALLVYFSDHGEDVFDSAGHKMLGRNEGAPTVPMYAIPFIVWGSDAWRATHPMNFGRE
                     VLERRYSTSDFIYTWADFAGMRFDDFDSSKSLIGKGFKEQPLWVGDPYGKVPLVDLRS
                     LMPKTQVAKPGKKMTHEAGGTVRQSD"
     gene            complement(22612..23475)
                     /locus_tag="PKW44_00125"
     CDS             complement(22612..23475)
                     /locus_tag="PKW44_00125"
                     /inference="COORDINATES: protein motif:HMM:NF025091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/PEP mutase family protein"
                     /protein_id="HQR50025.1"
                     /translation="MSLEQSHANISAGARLRQAIANNPRILPFIGVYDVFSASIAGRH
                     FDSLFVSGFGFAASHYGLPDIGFITWTDIVAFVQRLRTVLPGHNLLVDIDDGYCDPEV
                     ACHVVSVLEAIGASGVVLEDQKRPRRCGHFEGKQIMELDEYLAKLRAVLATRRDLVVI
                     ARTDTSDPDDMVRRVKAFAEAGADAVLIDGLPNLDVVRQLSKEVGRPFCFNQIAGGKS
                     PVYTQGELHEAGVSLAIYSTPCLFAAQSAIEAAMGDLKARDGTLAGSPVGVRDCNQVL
                     TDNLARRDSRV"
     gene            complement(23472..23732)
                     /locus_tag="PKW44_00130"
     CDS             complement(23472..23732)
                     /locus_tag="PKW44_00130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HQR50026.1"
                     /translation="MSGDRQQTLQHLIQFLRTIQKPGKPVESVGESDSLVATGLIDSL
                     AVVQIVLYLENTYGIDFAARGFDPDRLASMASILDLIEEASA"
     gene            complement(23732..25249)
                     /locus_tag="PKW44_00135"
     CDS             complement(23732..25249)
                     /locus_tag="PKW44_00135"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HQR50027.1"
                     /translation="MFVDLSVGNLAEPVSGRHWERQEIGRQVALRIARFQAQGLARGD
                     RVFLPFGNRLEFFAELLAIWRLGACAVPIDARLTPFEVQTLVAAAKPRLAVVDDATDA
                     HVIQALDEAGVGVVNTTDTGDQQAAVGLSRLDDDALILFTSGSTGAPKGVVHTHRSLR
                     ARWITLREALGTDAFARTLCLLPTHFGHGLICNCLFPWLSGQDLFITPPFRPDIIMRL
                     GALLDEHRITFMSSVPAVWKLALKISKPPQAGTLQRIHCGSAPLSAGVWEDIRRWSGT
                     RQVCNAYGITETGSWVAGLSEADCPAEDGLIGQGWGAVIKVLRTSDTSRPLSADDECT
                     PGESGFVWLNTPALMKGYFQRDDLTEQAVVDGWFLTGDIGLIDPRGRLVLRGRERDEI
                     NKGGMKIYPADVDAVVERFEHASDVCTFALDDAIYGQVVGMAVVMARQDDGTFRALHQ
                     WMKEHLAEPKMPVRWWVVEEIPRTSRGKINRDAVKAVCTDLPALDLTRILAEGAK"
     gene            complement(25268..26644)
                     /locus_tag="PKW44_00140"
     CDS             complement(25268..26644)
                     /locus_tag="PKW44_00140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR50028.1"
                     /translation="MQLSQLSQFHENAAVIAWWQARLGLALDWFTPSRRRMILAIGAV
                     WEGAKYPWKFLSKADELPVPTDGLGIALVILAIFALLWLCYRAAARFSALPGMVRRHP
                     QLTLHLFYWGMLAALWVTTPAAGLWRSVLLGVAAIFPYLLWRCGYVLLAGQYGRMAGT
                     RFSDHLLTLWPAYGGSETPYGKGLVYLSRLEAKTSEELARSQLAGIKLLLLCQLLRWV
                     RDLLEGVLYGPGNGLTVALGGFTLAIPQLGELVKAGGQAPIAASWASIYCEFVWQVLK
                     HAIRGHEIIGYLRLFGFNVFRNTYKPLLAESIVEFWNRYYYYFKELLVNFFFMPTFAQ
                     LGGVLRNRPQLRLFLAVMAAALFGNMYYHVLQEYDLLLQGDVFQALYNLRSRMFYCLL
                     LALGIYISMVREQRRSMQAQAPGTARRLLRMAGVWTFFSFIFIWNVRSDAPFLSRVNF
                     FLGLFGLA"
     gene            26759..28306
                     /locus_tag="PKW44_00145"
     CDS             26759..28306
                     /locus_tag="PKW44_00145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR50029.1"
                     /translation="MQSSLAIPTNKRLVPFLAILGILLGLWLAMLENGRINGDADLYL
                     EMASRFAQDDAQGALSLYQWPLLPWLMAQVHYLTGLDVESAGRLLGAIFFGIATWGFL
                     ELIREAGGSRNAILAGALLLFSSPYIVGDVLPMIMRDQGFWAFYLLALLFFLRFYRSL
                     SLADAMLWQLCVLVATLFRVEAVSFILLMPLLLPLRRDISWRSRLQSLGMAYLVPLMG
                     IILVILGGLLLPAAELQQKLGRLAEVQSSLQSVYHQISGGLAAKARIYGDQVLGKYMD
                     NYAMLGLCLTLFSVIAGKIIGASGWLGIVLATTGKRCQAPAMDPDARTVLLWAAGINL
                     LNLAVILLTAYLLSARYAVGMGFIVLVFAAFNLGGLCDRWLESGARRSRWLALGLLLA
                     ALLMAYHLVRSLAPHDADYNYEQAAVAWVKQHAAKDARIYYDGARLRYYAHAPWGGRA
                     DEWENNLPALQATPARFDYLLLTVKGKNPNQLALLEQLAQYRKVQEFRRDNSNAIVVM
                     VSRELPD"
     gene            complement(28317..30035)
                     /locus_tag="PKW44_00150"
     CDS             complement(28317..30035)
                     /locus_tag="PKW44_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase"
                     /protein_id="HQR50030.1"
                     /translation="MIILGLSGAVGHDPSAAILVDGKLIAAAEEERFLRDKHAKGKFP
                     YEATKYCLKEAGISPEQVDVVAFPYAEIPLSTPARWHYAMRHWYAPDRALDAIFNGNR
                     RFRRNRDKALELMQGLGLSNARFVPVEHHLAHASSAYHLSGFKEKTAIVGIDGKGEYA
                     TTFFGYGENGKIHKIKEFYDPDSLGGLYGAMTEYLGFEMLDGEFKVMGMAPYGDPHKY
                     DLSRLLEFDGKEFRVNTRLVNVVGLRRYKENGKGFYFSPTLVDWLGPKRDGDEIDHPY
                     IHYAAAVQQLLEDVALKLIDHYLGDIIRETGRIAMAGGVALNVKLNQRIIALPGVKEL
                     FVQPASGDAGTALGAATYVAAEKGDTVEPMKHAYLGPSYTTEECIAACEAHPEKPKWQ
                     RIGDASVKGAEILAAGNPLAWFQGRMEFGPRALGCRSILGDPSHPGVADRINAQIKYR
                     ERWRPFCPSMLDRAAADILGTDHPAPYMTFTFDVAQHWKSRIPEVVHEDGTARAQVVS
                     MDTNPRYYRLIEELERLRGIPVVLNTSLNRRGEPMICSPLDALNMFFGSDLEYLIMED
                     VLVTKA"
     gene            complement(30052..31527)
                     /locus_tag="PKW44_00155"
     CDS             complement(30052..31527)
                     /locus_tag="PKW44_00155"
                     /inference="COORDINATES: protein motif:HMM:NF018047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA family protein"
                     /protein_id="HQR50031.1"
                     /translation="MPRPDNLIENIASASGSYRVDLSGAPQLQCEAIVRLVPGRRLVC
                     RGSWDGRPVYAKLFFGSKAGRDAGRDASGVHALTEAGIATPELLFDGNIANGGGRVLV
                     FAEVTGSSAEVVWRTLAHDPAARLDLVKRLVQTVAKHHLSGLIQHDLYLKNFLVTEAA
                     VFTLDGDGIRCHAAPLGRRTALDNLALLLSKFDAVDDPWLPQVQQHYAAALGMALRPA
                     EIRTFPARVMAHRLRVARSYADHKVFRTCTDVIIEHRWNSYKAIARPQDSEELRALLD
                     NPEARLREANVRILKNGNTCTVLALPLKDRNIVVKRYNIKNFWHGLGRAWRPSRAAAS
                     WANAYRMNILGIPTAQPLALLERRWGSLRRQAYYVAEYVDATDATAFFADPAVSAERK
                     QYAVQNISRLLHKLWCLGLVHGDMKATNVLIGAEEQPLLLDLDAMRAVRCKWILRRGH
                     GRDLSRWMENWRNDPLTAEMMRQALEQVYQNDKWLRELLSV"
     gene            complement(31539..32333)
                     /locus_tag="PKW44_00160"
     CDS             complement(31539..32333)
                     /locus_tag="PKW44_00160"
                     /inference="COORDINATES: protein motif:HMM:NF018047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA family protein"
                     /protein_id="HQR50032.1"
                     /translation="MSDFIYPAYRQLLESNHLASFDQLWSYQAEWFEEPNNRRGGWSG
                     AGRVVLRLPDGGEAGVFLKRQENHQRPTLRHPLRGEPTFAREFRMLRFLQSRGVPAPV
                     PVFFGQRAARAILVSEELAGFRSLEDVTEDLFADDKRPPLSEQRALLRGVAAMVRKMH
                     DAGIHHRSLYPKHLFVNRKAAGDPLVAVIDLEKSRATLLATPRTIYDLATLNRHARAW
                     SKTARMYFFKHYLGIERLSPWHKLLCRLILKRSQRPKQYNSRSPNQ"
BASE COUNT         6539 a         9323 c         9879 g         6634 t
ORIGIN      
        1 ggtttcgtcg ccgccggttg ggcgcccgta cgggtggttg cggaacagcc aggccaccag
       61 caccatgagc aggccgccga gcaggaagcc gaccaagggc gagacgatga tgaacagcag
      121 gatcttgccg aagccgctcc agatgatggg cgcgatactg ccggccttgg cgacggtggc
      181 gccgaccaac ccgccgacca gcgcatgcga ggaagaggac gggatgccgt aaagccaggt
      241 aatgatgttc caactgagcg ccccgcacag cgcgccgaat atgacgtagt aatcgacgaa
      301 actcgggtcc accgtgccct tgccgatggt ggcggcgacc ttgagatgaa atagccacaa
      361 agcggcaaag ttgaaaaagg ccgcgaacaa taccgcctgc ttgggggtga gcacgccggt
      421 ggcgacgatg gtggatatgg aattggcccc gtcgtggaag ccgttcatga agtcgaatgc
      481 cagtccgacc agaatcagca gggcgaggat ccacaggttg atggtcaagc cttccatcag
      541 gaattctcga tcagaatctg ttcgatggtt ttggcggctt cttcgcagta atcgagtaca
      601 ttttcctgca gtacatagaa ctcccgcagc ttcatggcgg cccagacgtt gctgtcgtcc
      661 tggaacagcc gggtgatggc cttcttcagt accttgtcgg ccttggtttc gatgtcgtca
      721 atctccatac agagggcgac cgcttccttg cggcggttct tgtcccccag cgcgatcacg
      781 gcacggttga gccgcaggca ggaatcggcg ctgagggccg caagttcgcg cgcctcgggg
      841 gtggattcct ggatgttgta catgccgacg gcgttggcga cgctttgcaa ggcgcccagc
      901 accttgtcca gttccagcgt gagggtgtgg atttcgtcgc ggctgaacgg ggtgatgaag
      961 gatttgtgca gcagggcgat gacgtccgcc ttgatcttgt cgccgctttt ttcgttgaag
     1021 tccacttcct tgaccagcag ggcgatgtca tcccggttgt tggggccgag cgtattcact
     1081 aggcgcaatg tggcgttggc gcttgccacc acgcggtcgg agtgtgccgc cagtagatcg
     1141 aagaattccc tgcgttgagg cataaagccg ccgaacatga tagatgctct ccccttgata
     1201 tgtttcgttt attattcaga aactgaacgg acctatgcgt ccggtttatg aattgtaaca
     1261 aatatgcaat gaatcattgc gtaataaagc agccttgaga aagcaggagt gcgtcccgct
     1321 aatattttcg tgccataaaa atgtaacaca tgcctgctcg ttactaggct aatatccaag
     1381 gtcaaagttt taaccatagg ggggcttaat gaaaatttcc aatccggaat acttggctaa
     1441 ggtggaactt ctgaccgcgg atgagcggga gcgtctgctt tcgagaatgg caggcaaatt
     1501 gccgcgcaaa ctggagaggg acaagctgag cgagaacgag gctctggcta tccagatggc
     1561 gctggaggat gaacaacttc aggaatggcg cgaaaagatg cattccctga aggcgcaggc
     1621 gaaaggcaag gaaaaagcgg ataaggcaaa aaagggtgca gaaaagccgg caaaagccaa
     1681 aggtgcgacc ggcaaagccg gagaggctcc tggaaagcct ggcaccaaga tggtggccaa
     1741 gaaacctgtc gttaaggctc ctgtcgctag agttgcgacc aagcctgtcg ccaaacccgc
     1801 cgtgaagccg gcggctaaaa ctgctgcaaa gccaacagcc aaaacgacta aggcggcttc
     1861 ctgatcatct gcggcacatc tggtgcgcaa ggttttcggg aggtattcaa caaaaagccc
     1921 tctgcacgaa catgcggagg gcctttttgc ttgatggggg cagtgcctgc gttggccggg
     1981 aagcggatca gccgaacttg ccggtgatgt aatcctcggt ttccttgcgg ctaggtttgg
     2041 tgaacatgag gtcggtgtcg tcaaactcga tcagttcgcc cagatacatg taggcggtgt
     2101 agtcggaaat gcgcgcggcc tgctgcatgt tgtgggtgac caccagaatg gtaacttttt
     2161 ccttgagttc ggacatcaat tcctcaatgc tggccgtggc gatcggatcc agcgccgaag
     2221 tcggctcgtc gaacagcatg atttcagggt cggtggccag ggcgcgggcg atgcacaggc
     2281 gctgctgctg accgccggaa aggttgaaag cgacttcgtt gagcctgtcc tttacctcgc
     2341 cccagatcgc ggcgccgcgc agcgcttctt ccaccttgtc gtccatctct ctcttgttgg
     2401 tttcaccccg cacgcgtaat ccataggcga cattttcata gatggacttg gggaacggat
     2461 tcggcttctg gaacaccatg ctgatgcgca tgcgcacttc gattggatca acccccttcg
     2521 ctaggatgtt ggtgttatcc gggtgcatga cgatggcgcc gtcatagacg ttgcccggat
     2581 aaaggtcgtg catgcggttg aagcagcgca ggaaggtgga tttgccgcag ccggaagggc
     2641 cgatcagggc ggtaattttc ttctcgtaga tcggcaggtt gatgcctttc agcgccttgt
     2701 ggccgccgtt gtagtagaag tccagaccac gggcttccgc cttgaggggg gtgttattgg
     2761 tttgcatgtg cgagtcctgt taccacttga tgtttttgcg aatgcggtag cgcagccaga
     2821 tggctgtgcc gttcatcaac agcgtcacta cgatgatgac cacgccggta gccgcggcat
     2881 tcacatggaa tgcgtgatcc gggcgggaga gccagttgaa catctggatt ggcagcacgg
     2941 tgaagccgga ggagagccac tcgaaattga ggtaaggggg ggctggctgg atcgggctgg
     3001 gcggcaggaa tgcgatgtag gtcagcgcgc cgatggtgat gaccggagcg gtctcgccca
     3061 gtgcgcgcgc catgccgatg atgatgccgg tgaggatgcc gccgaacgag taatgcagga
     3121 tgtggtcgga caccatctgc cactttgtcg cccctaccgc ataagctgct tcacggatgt
     3181 gtaccgggat ggcacgaatg gattcgcgtg tgttaacgat caccaccggc aggatcagca
     3241 gcgccagcgt caatcccgca gtctggatgc tttgccccag acccaagtcg tacacgaaca
     3301 gccccagggc cagcaagccg tagatgatgg aaggtacgcc ggccagattg gtgacgttga
     3361 tttctatgac gtcgctgaac cagtttttcg gcgcgtattc ttccaggtaa accccggcgg
     3421 ccacgcccag cggcacagcg gtgaccaggg tgaccgacat caccagcagc gagcctaccc
     3481 atgcggaaag caggccggcc ttcgccgcat gacgcgaagg gaattcggta aagagcccgg
     3541 cgttgaagcg cgtgtaaccg tccatcaaca gatcgatgaa tagcgtcagc aatgtcagca
     3601 ggcctaccat caatgccagc aggccgatga gggcaaaaat agtgtcattg agcttgtggt
     3661 gacggatcat cgcccgcact gcatccagat ccttcagcgt agaggcttgt tgtccggaca
     3721 ttgttagtag gcctcgcgat attttttgcg agccagatgg cccagcaggt tgaggaacag
     3781 ggtaatcagc atcaacatca ggccggcagc gaaaatgctc tggtagccga tgctgccgtg
     3841 cggcaggtcg cccatggcca cctgaacgat gtaggcggtg atggtggcgg cgccatccat
     3901 ggggttgaac gtcaggttgg gctgctggcc agctgcgatg gccaccacca tggtttcacc
     3961 cacggcgcgc gagatgccga ggatgtaggc ggcgatgatg ccggaggtgg cagccggcgc
     4021 cacgacgcgt actgcggtct ggaaacgggt agcacccatg gcgtaagagc cttcgcgcat
     4081 gctcattggc acggcgcgca tggcgtcttc cgccactgaa ctgatatagg ggatgatcat
     4141 gatgcccatg acgatacccg ggcccagcat gttaaagctc ggcaggccgg ggatgatttt
     4201 ttgcagcaaa ggcgtgacga acaacagggc gaagtagccg aataccacgg tcggcacgcc
     4261 gaccagcaac tccaggatgg gcttgcagat ctcacgcacc ttgtgcgagg cgaattccga
     4321 caggtaaata gcgccgatgg tccccagcgg cacggcgacc agcaatgcaa tggccgaggt
     4381 ggtgaccgtg ccggagatca gcggcaggat gccgtagtgc gcatcttcga acagcggcgt
     4441 ccactgggtg tcggtgaaaa attcaacgag ggaaaccgtc ttgaaaaacg tcgacgattc
     4501 gtacagcagg atgcagacga ttgcgaccgt ggtggcaacg gcggagagcg ctgccagcat
     4561 caaaatgaat tcgatcagcc gctccttgaa gtggcggcga aagttcctgg cgagacggtc
     4621 gctgatgaca tgttctaaag ggttatgcag cacttgggtg gccatattca ctttgtagac
     4681 tcacgtattg tagccaattt ggctcggtaa aacctaatcc ggggcggcgt tggccgcccc
     4741 gggctatggc tattactgga tgcgctttag cagttcttcg atcttgacgc cgacttcagg
     4801 cacgccaccg aaaccagtac ccggcttgag caatttccag tgggcgtcca ctgccttgta
     4861 ctcgttggct ggcaggggaa tatacttcac ttccttgacc agtgtcgggg cgttggccag
     4921 atagaaattg atgaattcct tgacttcggg cttgaacgcc gccgctgtcg cattcacgta
     4981 gatgaacaga gggcgggaca gcggctgata ggtgccgttc atcacggttt ccgcagacgg
     5041 ctcgacagcc ggtgcacccg ccttggcgac gatgggaaca gccttcagct tgccctggtt
     5101 cgcttcgtaa taggcatagc caaagtagcc caagccgccg atgtcgccag agacgccctg
     5161 caccagcacg ttgtcgtcct cggaggcggt gaagtcgccg cggctggact tggccttgcc
     5221 aacgatggct tcggtgaaat agtcgaaagt gccggaatcg gcgcccgggc caaacagctt
     5281 gagcggcttg tccggccagg cggcgttgat ctgtttccag cttttgacgc tgccttgtgc
     5341 gccgggagcc cacatcttct ttaattcatc gaccgtcatg ctcttgatcc agtcgttctt
     5401 ggggttgatg acgacggtca gtgcatcgta ggccaccggc aattcaatgt attggacacc
     5461 ggcttccttg caggcgtcca tttccttctt caggatcggg cgcgaagctt cggagatatc
     5521 ggtttcgcca cggcagaact tcttgaaacc gccgccagtg ccggaaatgc cgacggtgac
     5581 cttgatggcc cctttcttgg aattctgaaa ctcttcggca acggcttcgg taatgggata
     5641 gaccgtgctg gagccgtcga tcttgacgat tttttcggcg gcgagcaccg tggcaggggt
     5701 gctgaacagg ccggagacaa cagcggcgat ccccagcgtt tgtgtgaact tgaattgcat
     5761 acaacctccg tggtttgggt aatcttaaga accgtgccac tgtaatgccg aagtattgca
     5821 gttttgtgac aaatgccgat cttgtgtcca atatggattc gctgcttatg aaaaatgccg
     5881 tttgtcttct tgaacatgcc gagcatccgc tgtgtcccgt ctataatcaa cggtttattt
     5941 gacttgcttg taattcatga tagagtccat tatcggaaaa actgccggaa gtgaaatcat
     6001 ggccaaaaac aagctgtcag aagacttcgc cgcctatagc cagtggcgtg gcgtcttgat
     6061 ggatgggata tgtgattacc gcagctggtt gaacgagcag gagctcaatg acgcgcagat
     6121 tgacctgcgg attcagcacc tgcttgaccg gctgcgtgaa gacaagctca acgttgcttt
     6181 cgtcgccgag ttctctcgtg gcaagtcaga gttgattaac gccatttttt tcgctgatta
     6241 cggccagcgt ctgctgcctt ccagtgccgg gcgcaccacc atgtgcccca ccgaatttct
     6301 gtatgacgcg accaagcaga cgtcgttgca attgctcccg atcgagacgc gctcctccga
     6361 tgcgacgacg accgaataca agcgctatcc cgatgaatgg acggtgttcc agttcgatgt
     6421 tgcatccaac gaaagcatga tcgaagcctt caaggaggtg agcagaacca agcgcgtttc
     6481 agtcgaggaa gccgagcgct atgggttatt cgatcccgat agcaatgacg acgccatggc
     6541 gctcaatgag gatggttcgg tggatatccc atgctggcgc tatgccatca tcaatttccc
     6601 ccatcctttg ctggaacagg gtttggtgat cctcgacact ccggggctga atgccatcgg
     6661 caccgagccc gagttgacgc tgaacatgct gcctaatgca catgcggtat tgtttatcct
     6721 ctcggcggat accggcgtta ccaaatccga gattgatgtc tggcgacact acatcagcgg
     6781 gacgcgctgg aagcagaagg cgcgtatggc cgtactcaac aaaattgatg gcctctggga
     6841 tgagttgaag gacgagcagc aaatagaagc ggagatcaac aagcaggtga ccggaagcag
     6901 cgaattgctt ggcctcaatg tcaaccagat attcccggtt tcggcgcaga aaggcctgtt
     6961 gggcaagata agcggggacg accagcttct ggcgaggagc cgtttgccag agctggaaag
     7021 ggcgttgtcg gacgaattga ttccttccaa aaaggaaatc gtgcgtgaca acacgcaaag
     7081 cgaattggac gatttgctca acaactccca gctgattctc gatgcccgcc tcaaggggat
     7141 catcgagcag cttgacgaac tcaaaggcct gcgtggcaag aacgaagatg tcgtcaatca
     7201 catgatgaac aaagtcaaga acgacaagga gaatttcgag cgcgggttgc agcggttcca
     7261 ggcgctgcgc agtgtattct cgcagcaagc taatgccttg ttcactaatc ttggcatgga
     7321 ggccttgcgg tctgatgtgg gtgaaacccg caatgccatg gtttccgcca atttcaccaa
     7381 gaacatacgc gcggcgatgg acgatttctt cttcaggctg aaggagcggc tggtcaagtc
     7441 tgatgagcag gtcggcgaaa tcaagatcat gatggatgcc atgtaccaga aattttcaca
     7501 ggagcacggg ttgcggcatg tgacgccgcc cagtttttcc atgctgcgcg accagaaagg
     7561 cgtggcgaaa ctggagcgca cctaccgcga acagttcaac acgaccttca aaatgctgac
     7621 caacgagaaa ctgacgctga ccagcaggtt ctttgagacc ttggccagtc gtgtgatcaa
     7681 tatttatgaa gtggctaacc aggacaccga gaattggctc aaggctgtca ttgcgccgat
     7741 ggagtcacag gtgagggagc accagctgca actgcgccgg cgcctggaaa gtatcaagcg
     7801 tatctacaaa gctaccgata cgcttgaaga ccggattgcc gagatggaga gtgtcgagca
     7861 gagcatccac agacagatcg cggacctgcg tgagttgcag catcagatca atcaagcgct
     7921 cagctttgat cggcaagagg aagtcggggt aaaggcggca tgacgcccat actgcttttc
     7981 ctgttcgcgt tgacactgct gtttgtccgt ctttccgcgg tgggtgtgct ttgggcgata
     8041 aaaagcggcc agttcgatga cctggagggg ccggctcagc gcatcctgat ggacgacgac
     8101 gaccccatga ttccatttaa gagcttgaag tccgaaagca cccggcataa aataggcgtg
     8161 cgacagtaac tcaaaaactt taggagccgg ccatgcatat tttttcctgg gaaaacctgc
     8221 caatgactat tatcgtaggc ctgatcgtct ggtcttacgt cagtttcttg tcggtcattc
     8281 tggcctgatt cggaagctta tccgataaaa cagaaacggc tgcagttgca gccgtttctg
     8341 ttttatcaag ggtggagatc aggccgcaag accaataacc cccttcattt tttgcagtgc
     8401 cttctgctca atctggcgaa tgcgctcggc agaaacaccg tactcggcgg ccagttcatg
     8461 caatgtcgcg gcattatctt cccttagcca gcgcgcttcg acgatatgcc ggcttcgctc
     8521 atccaggcta ttgagagcca tttccaagcc agaggcatgc agaaccccaa tctgcttttc
     8581 tgccagaaat tctgaaggtt ccggacgctg gtcctccagg aaagcgatcg gactccaacc
     8641 ttcctcgcca tcttcgtcgc cgtgcgactc cagagcgatg tcgtgaccgc ccatccgaga
     8701 ctccatctcg gctacgtctt ccggcttcac attcaactcg cgtgcaattt ccgcgatttc
     8761 atcactgtgc agactgccgc tgtgctgctt catgctacgc aggttaaaga acagtttacg
     8821 ttgcgccttg gtagtggcga ctttgaccag gcgccagttg cgcaggatga attcgtgaat
     8881 ttcagccttg atccagtgca ctgcaaagga taccaagcgc acgccgcgtt cgggatcgaa
     8941 gcgacgcaca gccttcatca ggccgatatt gccttcctgt atcaggtcgg cctgtggcag
     9001 tccgtaaccg gagtagccgc gcgcaatact gactaccagg cgtagatggg agaggattaa
     9061 ttgcttggcg gcatccaggt cgttatcgcg ctgtagccgc gtggcgcagt catgctcttc
     9121 ctccgcgcta aggaagggaa aagacctgac gctctggata tagtgttcca agctgtctgc
     9181 cgttgccggc attggtaagg atagggaata actcattagc tgttgacctc ccgatggctt
     9241 gtattttagc actcaggatt agagagtgct agtcgggaaa agttctcgcc gcttcaaagc
     9301 gggtcaatgt ccgatagtgc gcggcttgcg gaccagtaag cgcctagcca gcccagcccg
     9361 acggctccgc cgaccagcgc cagcgcagct tgccagtcag gcatctgcag tcggaaatcg
     9421 gaggcgtaga gacgggcgag gtcgtcgacg gataaattga acagatcgac cacgccccat
     9481 aggaacagcc aggccaccaa cccgccgcct aggccatata gcgcgcctgc gtaaaggaat
     9541 ggccgccgga tgaagcgtcc ggtggcaccg atcagcgcgc tcacttcgat ttcgtcgcgt
     9601 tgggtaagga tttgcaagcg aatggtgttg ccgatgacag cgagcagggc gaatcccagc
     9661 agtccggcca gtacggcaac ggctttcttg ccaagttcga ccagtgaata cagacgcttg
     9721 acccatgccg tgtccaattg cgcgtgtgct acctccggcc actgctgcat ctcctgctgc
     9781 atctgctcga taacctccgg ggtgtcgttg tctggaagga tgatgtaagc atcgggtagc
     9841 ggattttgtt ccagtccatc ggcaatgtcg ctcagcccgg cattctggcg cagtttctgc
     9901 caggcttcgt cgcgagggac gaagtggtag gagcgaatgc catgctgccg gcgtaggcga
     9961 tccccgatgt cctggatggc ttcaggcttg gcgtcaagcg cgacgaacag gctgatctgt
    10021 ggctcactcc ggacgtcacc ggaaaagcga ctgaggttgt ctactgccac gtaaaggagc
    10081 gcaggcagcg acagggtcgc tcccatgact cccagcatca tcagcgtagc aagccggttg
    10141 cgccacatgc ggcaaagcac ctggcggaat gactggcgat gctgactgag ccactggctg
    10201 atcattgcag tactccatgg tcgaggtgca tcactcgacc accgcttgcc gcggcgccat
    10261 gggtgtcatg cgtggcgatg agcacagtca cgccaacctg gtgaaaggcc tggaacagtt
    10321 tcatgatctc ggcggcgtag gtttcgtcca ggttcccggt tggttcgtcg gccactagaa
    10381 tggacggccg actgaccacg gcacgggcga tggccaggcg ctgctgctct ccaccggaga
    10441 gcgtcaccgg catggccttc tccttgtcca gcaggccaac cttgtccagt gcggcacgaa
    10501 cacgtttggc ggcctcctgt ccgacgatgc cgttgatgga cagcggcagg gcgacattct
    10561 cgaatacatt gcggtcgtat agcagcttgt ggtcctggaa caccatgccg aatttgcggc
    10621 gcaggtaggg aacagccgcc gggcgcagct tgctgacatt ctgctgaccg atcagcaccg
    10681 tgccggaggt gggccgttcc agcgcagcca ccagcttgag cagggtgctc tttcctgccc
    10741 cggagtgtcc ggtgatgaac accaattcgc ctttgtcgat ctcgaatgac acttttttca
    10801 gtgcctcgaa accgccgggg taaagtttgc cgacctggtc gaatctgatc aagtgaacaa
    10861 ggcctccaca aattcacgtg cattgaacgg ctgtaaatcc tcgatctgct cgcccacacc
    10921 gatgaaacgg atggggatgg gccttgcctg ggcgatggca gcaatgacac cgcccttggc
    10981 ggtgccgtcc agtttggtga gcgccagtcc agtgacctgc aaggcgtcat cgaaagcctt
    11041 gacctgcgtg acagcgttct gtccggtatt ggcatccagc accagcaata cctcatgtgg
    11101 tgcgccgggg agcgccttgt cgatcacgcg tttgaccttc ctgatttctt ccatcagatt
    11161 aagttgtgtc ggcaggcgtc cagcggtatc cgccagcacg acgtcgatgc cgcgcgctct
    11221 ggcggaattg accgcgtcaa aaatcaccgc cgctggatct ccaccgcttt gcgagaccac
    11281 atggacatca ttgcgctcgc cccaggcttc aagctgttcg cgcgctgcgg cgcgaaaagt
    11341 gtcgccggcg gcgattagca ccgattttcc ctgggcttgc agcagccagg ccagtttgcc
    11401 aatggtggtg gtcttgcccg cgccgttgac accggccagc atgatgacat aaggccgcga
    11461 cgaaccggcg tcaagtggct gcgccagcgg ctccagcaaa cggatcagtg cttcctgcaa
    11521 ggcgctgcga agctgcgaag tgtcgctcat cgcctggcgt ttgacttcct tgcgtacttc
    11581 ttccagcagc gcttgggtgg caccaacccc gacatcggca gtaagcagta tggtttccag
    11641 ctcctcgtac accgcctcgt cgatcttgcc accaccgaac agggtgccca acgagttgcc
    11701 aaggttgtcc ctggtgcgtg ccaatccttg tttgagccgt tccccccagg aaggggacgt
    11761 gggttccggt gcagtggcgg gttgcgggtc ttttttgaag aaattgagca tgggtgctat
    11821 tttagtctga atgctaactg cacgccattt tgaagcgatc attgcggcca ataagccgtg
    11881 cgccaatctg ctaaaatctc gccatgttta cctgaatcat tcaggccgtc ggccaaattg
    11941 agcacatcga acctgcgggc aatgatgtgc gtttatccat tgccagcggc gcccttgaca
    12001 tgggcgatgt ctgcgtcggc gattccattg cgacaagtgg cgtttgcctg acggtgatca
    12061 cttgcgacga aaaatccttc caggtgcatg tctccagaga aaccctgtct tgcaccgtcg
    12121 gactggatac cactaaaccg gtcaatctgg aaaaagcgct gcgcctggcc gaccggttgg
    12181 gtggtcatct ggtcagtggc catgtcgatg gtgtcggtca ggtggtgcgt ttcgacccgg
    12241 tgagcgactg catgctgctt tgcatccgcg cgccgcacgc actcttgcgt tatatcgcgg
    12301 tcaaaggttc catcgcagtc gatggtgtga gcctcacggt gaatacggtg gaacacgatg
    12361 ctttcagcat caatctgatc ccgcatacgc tggagatgac taccttgcgc catctgggtg
    12421 ttggcagccg tgtcaacctt gaggttgatc cgatcgcccg ctacgtagag cgtatgacgc
    12481 agtgggccaa cgaaggcgag ccggcatgat ttcagccgtc accgaaatca tcgatgaaat
    12541 tcgccacggc cgcatggtgg tgctggtaga tgaggaagat cgcgaaaacg agggtgacct
    12601 ggtgctggcg gcacagttcg tcacgccgga acatatcaat tttatggcgc gccatggccg
    12661 cggcctgatc tgcttgaccc tgaccgagcc gcgctgccga cagctcaacc tgcctttgat
    12721 ggtgcgcgac aatggtgctc gcatgggtac caacttcacc gtttccatcg aggcggctca
    12781 gggcgtgact accggcattt cggccgccga ccgcgcgctc actatccagg ccgccgttgc
    12841 ccgggatgcc agggcagctg atatcgtcag tcctggccat gttttcccgc taatggcgca
    12901 gaatggtggc gtactggtgc gcgcagggca caccgaggct ggttgcgact tggccacgct
    12961 ggcaggactg gcgccggcag cggtgatctg cgaaattctc aaggatgatg gcagcatggc
    13021 ccgcctgccg gacttgtgcg aattcgccga gcaacacagt atcaagatcg gcaccatcgc
    13081 tgacctcatt cgctatcgca gccagaccga acggctggtg gagcgtgctg ctgaacgcag
    13141 agtggccacg ccctacggcg ctatgcggct ggtggcctat tacgacaaga ctgccaacga
    13201 aacgcatctg gtgctggtca agggcgagcc tggcccggag caggaagtac tggtgcgggt
    13261 gcatgagcca ttgtcggtat tcgacttgct ggacggcgac agccagaccc actcatggag
    13321 tgtgctggac gccatgaagg taatccaaca ggcaccatgc ggcgtgatga tcctgctgcg
    13381 ccgcaacgag gatggccagc aactggtgga acgtatccag catgccgatg aaccggtacg
    13441 ctccaggcaa gatctgcgcg attacgggat tggctcgcaa atcctgcttg acctcggcgt
    13501 gcggaaaatg aaattgatgg cggtgccgcg caagatgccg agcatggcgg gcttcgggct
    13561 ggaagtgact ggctacatgg aacctggcaa ttcatgagat aatcgcaatc gtggaaaacg
    13621 tcaaccttac cggacacttc ctcatcgcca tgccagccat ggccgatcct tactttgccc
    13681 gttccgtcac ctacgtctgc gagcacaacg ccaatggcgc catgggtgtc gtcatcaatc
    13741 gtcccatcga catgaacctg gacgacctgt ttggccagat cgaggtggaa ttgatcgaca
    13801 aggatctagg acaaacgccg gtacatttcg gcggcccggt acagatcgac cgcggcttcg
    13861 tgctgcacca accggtgggc caatggcagt ccaccatcgc cgtcagtcgc gataccggcc
    13921 tcaccacttc gaaagacatc ctggaagcca gcgcgcacgg cgacgggcca aagaagatgc
    13981 tggttacact gggctatgct ggctgggcgg cgggacagtt ggaagaagag ctggggcaga
    14041 atgcctggct gacagtcccg gcgcaggaat cagtgatttt cgatgtggcc agtgaagaca
    14101 agttcagcgc tgccatgggc ctgcttgggc tggattttgc cagcctctcg gaagaggcgg
    14161 gacatgcctg aggccggtac ggtgctcgcc ttcgacttcg gcgaacggcg catcggcgtg
    14221 gcactcggcg attttgggct gggcattgct cacccgctcg ccaccataga caccgaaagc
    14281 aacgatgcac gcttcaaggc tattgcggcc atgatcggcg agtggaagcc ggcgcaactg
    14341 gtagtggggt tgccagccta ccttgatggg gcggaacatg agttgagccg cctgtgccgc
    14401 aaatttgcca accgcctgca cggccgcttc ggtctaccgg tggcgctggt ggatgaacgc
    14461 ttgacctccg ccgaggccag ccagtcgtta cgcgaggctg gcgtcggtag ccgccggcag
    14521 aagccgatgc tggatcaggt ggcggcgcaa cacattttgc aatcctactt tgacgcgcac
    14581 acccatgccc atgctgcctg acgccgagtt tctggtcgaa acactggcgg aacaaatccg
    14641 cccgctactg gggcagaacg ccgctctggt tggtatccac agcggtggcg cctggctggc
    14701 gcagcgcctg caacagcaac tgggcggcga catcccgctc ggcacgctcg atatctcttt
    14761 ctaccgagac gatttcagtc gcatcggcct gcattctcaa gtcaagccgt ccagcatcaa
    14821 cttcgaggta gagggctgcc acatcatcct ggtagatgac gtgctctata ccggccgtac
    14881 catccgcggt gccatgaacg agctgttcga ctatggccgc cctgccagga tcacgctggc
    14941 ggtactggtg gatcgcggcg ggcgcgaaat accggtggaa gcgcagatcg tcggcgcccg
    15001 gctggaactg gatcccggcg agaacttgga actggcacgc gacgagaccg gcaaattcca
    15061 cttcatcctg catgaggccg catgaataac ccccaattga caaaagacgg caaactcaaa
    15121 cacctgctct ccatcgaggg cctaccgcgc agtattctca accagattct cgacaccgcc
    15181 gaatccttcg ccggcgtttc cgaacgcgaa gtgaagaagg ttccgctgct gcgtggcaag
    15241 acggtgtgca acatcttctt cgagaacagc acccgtaccc gcactacttt cgagatcgcc
    15301 gccaaacgtc tgtcggccga cgtggtcagc ctcaacgtcg gcacctcgtc gcaggccaag
    15361 ggcgagacca tcctcgatac ggtggataac ctgatcgcca tgcacgcgga tatgttcgtg
    15421 gtgcgccacg cgcaatccgg cgccgcccat tttatcgcac gccacgtgcc gccgcacatc
    15481 cacgtcatta acgcgggcga tggccgccat tcgcacccca cgcagggctt gctggacgtg
    15541 ttcaccatcc gccgccacaa gcgcgaattc cataacctgc gcatcgccat cgtcggcgat
    15601 gtgctgcact cgcgcgtagc gcgctcggaa atccatgcgc tgaccacgct gggcgtgccg
    15661 gaagtgcgcg tcattgcgcc aaagacgctg ttgccgacag acgtcgagcg cctcggggtg
    15721 caggtctacc acgacatgcg cgaaggcctc aaggacgtgg acgtggtgat gatgctgcgc
    15781 ctgcagaacg agcgcatgtc cggcgcgcta ctaccttccg cgcaggaata cttcaaatgt
    15841 tacggcctga cgcaggaaaa actggcgttg gccaagcccg acgccatcgt catgcacccc
    15901 ggaccgatga accgcggcgt ggagattgac tcgtcggtgg ccgatggcag ccagtcggtg
    15961 atcctgcctc aagtcactta tggtatcgcg gtgcgcatgg cagtgatgag catactggcg
    16021 gggaactcgg aacaatgaaa attcatatca agaatggccg cctgatcgac cccaggaacg
    16081 gtgtcgatgc tgtcacagac ctttacattg cggcgggcaa ggtggttgcc gtgggccgta
    16141 cgccggtgga cttcagcgtc aatcagacga tcgatgcttc tggcctggta gtttgtccgg
    16201 ggctggtgga cttgtcggcg cggctgcgcg aacccggttt cgagtacaag gccacgctgg
    16261 aaagcgaact gcaagccgcc gttgccggcg gcgtcaccag cctggcctgc ccgccggaca
    16321 cggacccggt gctggacgag ccgggtctgg tgcagatgct caagcatcgc gccaagctgc
    16381 tcaatctggc gcatgtttac ccgctggggg cgctgacccg ccaactggaa ggcaagcagc
    16441 ttaccgaaat gtgcgagttg cacgaggccg gctgcgtcgg ttttgcgcag gccgatgcgc
    16501 ctcttaccga tacgcgggtg ctgctgcgcg ccatgcagta cgccgcgact ttcggcttca
    16561 ccgtctggct gcgcccggaa gacgcctttc tggcacaggg gggcgtggcg catgatggcg
    16621 agattgctgc acgtcttggc ttggccggta ttcctaccgt cgcagaaacc gtcgccctgg
    16681 caacgatact gcgtctggcg gccgaaaccg gcaccaagct gcacctctgc cgcctgtcca
    16741 cgcgtgccgg catcgagatg gtgcgcaaag ccaagcaaga cggtcttgcg gtgacctgcg
    16801 acgttgccgc caaccatctg cagttaaccg agcacgacat cggcttcttc gacgcccact
    16861 gccacctcaa gccgcctctg cgaacccagc gcgacctgga ggcactgaag cgggggctga
    16921 gcgatggcac catagatgcc gtgtgctccg accatacgcc ggtggacgac gacgccaagt
    16981 tgctgccgtt tgcagaagcg gaggcgggcg ccacgggcct ggaactgctg ctgccactga
    17041 ccctgaaatg ggcggcggac acaggcgttg ccttgccgga tgcgcttgcc cgggttacct
    17101 gcatccctgc ggacatactc gggttggatg ccggccacct tggcgcaggc agtgatgccg
    17161 acatctgcat cttcgacccg cggcaatact ggaaagtgga agcgaaatcg ctggtcagtc
    17221 agggcaagaa tacgccgttc cttggcctgg agctggccgg cagggtcagg catacactgg
    17281 tgcatggtca agtggtttac cagccagcct gatggccaaa tactcacggg agcgctttgc
    17341 catgtgcatg cagaaaatcc tgatggcctg cttgctgccc ttggctctgg ccgatgcgtg
    17401 ggccggcgat gtccagcccg gcctgtggcg tatctcgctc agcatgagtg ccgcgggtac
    17461 ggatcaggtc tttggcccct tcgagaaaac ccagtgtttc accgaggccg atgcgcgcga
    17521 tccggggaaa ctgttcgccg atattggcgg aacgtgcagt tacggcaaca aaaactacca
    17581 aggcaaccat ttcagtttca ccatccagtg cggcggtgcc ctgccaatga tgggttccgg
    17641 atcggtggac tacagcgcca cctcattcca gggggaattg aacctgaagg cgaacattcc
    17701 agataccggg aacgtggaaa caaaaagccg ggtgtcgggc agccgaatcg gcggctgttg
    17761 agtcacagaa aatttcccat ggccagaatc gccactcccg gcgccagaat agcgctggcg
    17821 gttcattggg gcgaggtgcc cctcctactc gcttaagact tcatccgcag caattcttgc
    17881 ggcctgcagc ttgtgcgcct gttgttggcg ctgctgttgc gaaccggacc gcaaaatttc
    17941 cctgattgcc agattgagtt gtcccaactt ggcgctggcg atggcgtggc gggcaattcg
    18001 gcgcacgtcg cgggcgtcgt agtgtttcct gcagaaaaga tagaaccccc ttcttttccg
    18061 gagaaacttg tccagcggga tggcgccggc ttcgctggca ccgccgacat gggtgatctt
    18121 cgctgcactg caatagccga tcgcatagcc ggcttttctg acacgcaggc agatgtctat
    18181 gtcttcgccg tacaaaaaga aatcggggtc gaacccgtgt atctgctgga atgtgctttt
    18241 tctgagtatc aggcaggcgc caagtatcca ggcaatcttt ccgggcaaaa cggccaggcc
    18301 tttcgtaaat ttcagccgat gctgggaagg ataggttttc ctgggcaaca cgtatttatt
    18361 tttggacggc tcatgaattt ccggtccggc gatgcctacg gcttggtttt gttgtaggta
    18421 ggcgaccagc gtagtgacgg cgccatcgcc ttgcagttgg gcatccggat tgatgagcag
    18481 cagaaattcg ccctgggcct taacggctgc gagattgttg gctcgtccga agccgagatt
    18541 ttcccggctc tcgatcaggg tgatgcagcc gccgcaggcg tcgcgcagga gtgcgatgct
    18601 gtcgtcctgg ctagcgttgt cgaccacgat aatttcgagg ccatcgacct gctgtgcctg
    18661 cagcgaacgt atgcagtcaa ccgcatggct gccggaattg tagttgacga tgatggccga
    18721 tacctggaat ttgctcatgc gttcgtgccc gatccgtaaa ctactttttc tctgccttgc
    18781 gggtgaaccg ggaggtgtcc ttgatcgcgt cgaggatgag ggtatggttc ctgaccagcg
    18841 cttcggaagc ggcctggcca ctgtagggtg tgtcgtcggg cttggtcgga gaccaatggt
    18901 ccgccccgcg ggtgtcataa gggatgcggt acttgatgtc gtcggtgatg acgctgatgc
    18961 tgtgccagtc gctgctgacg acgtaagggc ggtgtccggt gtcgaacagg ttgcgtccca
    19021 gagaatagcc tgacgcatct ttgggcgctc ccagaatttg catcagggtg gtaccgacat
    19081 ccaggtggct ggtgactctg tcgatctcgc tgtgctgcat tcccggcatc cacaccacca
    19141 tcggtgtcag tatctgctgc tcgacgaagc tggaattgtg tccccagaag cccttctcca
    19201 ggaactcctc tcggtggtcg ccgtagaaga aaaagaaggt attgtccagc agccctttct
    19261 gttccagttc atcgataatg cgtccgattt gaacatcgag ccagtgcgca gagttggtat
    19321 agcggttgag caattcatct atcctgggcg ccagagaagc ccgcgtcatg gtgctgtaat
    19381 tgacgttctc caggtaaggc cgggcaatga tggcggaatc cggaaaagag tagcgggcgt
    19441 gcgtggattc gaaaaagaaa aagctcatga acggccggga aggatcgcgc ttctgcaaaa
    19501 agttgatcag cgccgacgca ttgtcctggt cgcgctgcca cggttccttg tttgccggtt
    19561 cttcatgcag ttgttctagc gggatgttgg cgaacagggt cttgttgaat tccgggtagg
    19621 aaaatgcggc actggtgcgc aggtcgaact ggtagccgag ttcctgcaag cggtccatca
    19681 gcagagggct tttctgcgcg tacaggaagc tcgaccagta cgatccatac aggccgtaga
    19741 acatgccgaa caagccttcg cgcgtgccgt tgccgctgga gtagtggtgc ttgaaatgct
    19801 gtccgcgttg tgccaggcgc caggtgttcg gcatgatttc cggcgtcagg cggtcccagc
    19861 gcagcgattc ggccaccagc atcacgatgt tgggcggctt gacgaccatc tcgtacgcca
    19921 ccggctgcaa gggataattg aggcgcgtgg catccagcgt tacgctcaac ttgttctggc
    19981 gttgcgggtt gacgccgaat ttggacgcca cgccgcggaa agtcgtccgc ccgtagaatg
    20041 gatagacccg cgaagtgtcc agaatcgcgc catcgtttct gatgtcgctc acgccataga
    20101 tcacgcgctc gcccaccatg gccagcacca gcaatgccag caccagatgc cagcgtagga
    20161 aagaccggcg tttcgaggcg aaccaggtgc cggccttcca tagcgcggcc tgaaccagag
    20221 cgtggccaag cgcgatgagg gcaatggaca cataggtgtc gttgccgccg cccagcgaag
    20281 cgatcccgcc gggagtggtg agcaggttca ccacgaagcc gttgaaatgg aagctgtaca
    20341 ggtcgtagat gacactgtcg gagcggatga acaagatgca caggctggtg ccgaggatag
    20401 cggccgcggc cgtcagcatg cgtcgcgccg gccagaacct tgccagcagc acggccggca
    20461 acagacccgg cagcagatag agcagactgt agttggccca ggtgagcaca tggaaaagca
    20521 gcggcaggct gaacgcgatt tccggattgg ccatgcggaa ataggcccag gtcacggtca
    20581 gcatgtgcag ccaactgagc aaggcgaaat ttttcaaagc ggcggttagg tgagtgcgca
    20641 tcaaatcatc ctggcggggt atccctgcgt tgcggccggt agtttatccc aaaactgatg
    20701 gacgcgatga atccatccgt gccggcgcag gtgtgatggg tcttggcatc agctataatt
    20761 ctgcccgact tccactgtat tcaaccgtgg cacggacgtc ggcgtccgtg cgcatctttc
    20821 ctaaaaaggc aggcacatgg ctttcgcgaa ccaggcaaat accacccgaa acggcatcga
    20881 ctgggccggg atcggctggc tctacctttt cttctggtat ttttccggac tcaaccacgt
    20941 gctgctgcag ttcaccggta ctactatttt ctttggcttc cgccagtccg tgttcatgag
    21001 cctgctctgg ctggtgccgg tgctgctgtt cccgcacagg accagggcga tttcggcggt
    21061 gatcggggtg gtgctatggt tcttttccct gttcagcctg ggctatttct gcatctacgg
    21121 ccaggagttc tcgcaaagcg tcattttcat catcttcgag tccaaccagg cggaatccag
    21181 cgagtatgtg gcgcagtatt tcagctggtg gatgatcccg gcattgctgg tttacagcgc
    21241 cgtcgccgtg tatctgtgga agcgcatacg cccggtattc ctcccgcgcg gactggcttg
    21301 ggcgatcagc ctgctgatcc tgttttttct gtttggctat ccgctataca ggcaggcaat
    21361 cgtcaagcag gctccggccg aaagagtgcg ggagcaaatg gaagaccgca tggaaccggc
    21421 ggtgccctgg cagatggtga tcggctatct gcactaccgc gagcagttgg gcaacatgca
    21481 ggatttgctg ctgaccaacg ccaaggtccc ccctattgcc aacctggttg atgcgaacac
    21541 cgggcaaccg gcgacgctgg tgctggtgat cggtgaatcc accaaccggc agcacatgag
    21601 tctgtacggc cacccgcgtc cgaccacgcc aaagctggat gccatgcgcg accagttgct
    21661 ggtgttcaac aacgtattct cgccgcgccc ttacaccatc gaaaccctgc agcaggtgct
    21721 gaccttcgcc gaccagaaaa atcccgactg gtacctcacg cggccgtcga tcatgaacat
    21781 gatgaagcag gcaggctacc agacctactg gattaccaac cagcagacca tgaccaagcg
    21841 caacaccatg ctgaccacct tttccaagca gaccgacaag cagttctatc tgaacaacag
    21901 ccgcgcgcag aattcccgtc agtacgatga cgacgtgctg gaacccttcc agcaaatcct
    21961 ccgcgaaccc gcgccgcgca agttcattgt cgtgcatctg ctcggcaccc acatgaaata
    22021 cgagtatcgc tacccgcccg agtatgagcg ttttaccaat cgcgatggcg tgccacaggc
    22081 gctgacggac gagcaggcgg cgatctacaa tacctacgac aacgccgtgc tatttaacga
    22141 tcacgtggtc tccagcctga ttgaggatct ggccgcgaca aagtcgaagg cgctgctggt
    22201 gtatttctcc gaccatggcg aggacgtgtt cgattctgcc ggacacaaga tgctgggccg
    22261 caacgagggc gctccgaccg tgcctatgta cgcgataccc ttcatcgtct ggggttccga
    22321 cgcctggcga gccacgcatc cgatgaactt cggccgggaa gtgctcgaac gccgttacag
    22381 cacctccgac tttatctaca cctgggctga ctttgcgggg atgcgtttcg acgacttcga
    22441 ttccagcaag agcctgatcg gcaagggttt caaggaacag ccgctgtggg tgggcgaccc
    22501 ttatggaaag gtgccgctgg tagacctgag atcgttgatg ccgaaaaccc aggtcgccaa
    22561 gccgggtaaa aaaatgacgc atgaagctgg cggaactgtc cgccagtctg attaaacgcg
    22621 gctatcccgg cgcgccagat tgtcggtcag cacctggttg caatcgcgta cccctactgg
    22681 acttcctgcc agggtgccgt cacgtgcctt gaggtcaccc atggcggctt cgatggccga
    22741 ttgggcagcg aacaggcagg gggtgctgta gatggccagc gatacaccgg cctcgtgtag
    22801 ttcgccttgg gtgtagaccg gagacttgcc gccggcgatc tggttgaaac agaaaggacg
    22861 gccgacttct ttcgacagtt ggcgcacgac atccaggttg ggcaggccgt cgatcagtac
    22921 ggcatccgcc ccggcctcgg cgaaggcctt cacgcgcctg accatatcgt cagggtcgct
    22981 ggtgtcggtg cgcgcgatca ccaccaaatc gcggcgggtg gcgagcacgg cgcgcagctt
    23041 ggccaggtac tcgtcaagct ccatgatctg cttgccttcg aagtgcccgc agcggcgcgg
    23101 gcgcttctgg tcctcgagca ccaccccgga cgcgccgatg gcttccagta ccgacaccac
    23161 gtggcaggcc acctccgggt cgcagtagcc gtcgtcgatg tcgaccagca ggttatggcc
    23221 cggcagcacc gtgcgcagcc gctgcacgaa ggcgacgatg tcggtccagg tgatgaagcc
    23281 gatgtccggc aagccataat ggctggcggc gaagccgaag ccgctgacga acaggctgtc
    23341 gaaatggcgt cccgcgatgg aagcggaaaa cacgtcatag accccgatga acggcaggat
    23401 gcgcgggttg ttggcgatgg cctggcgcag gcgggcgccg gcggaaatgt tggcgtggga
    23461 ttgttccagg ctcatgcgct ggcctcctcg atcaggtcaa gtatgctcgc catggaggcg
    23521 aggcggtcgg ggtcgaaacc gcgggcggcg aagtctatac cgtaggtatt ctccagataa
    23581 agcacgatct gcacgacggc cagcgagtcg atgaggccgg tagcgaccag actgtcactc
    23641 tcgccaacgc tctccaccgg cttgcccggc ttctggatag tgcgcaggaa ctggatcagg
    23701 tgttgcagtg tttgctggcg gtcgccgctc atcacttcgc cccctctgcc aggatacgcg
    23761 tcagatccag cgccggcaga tcggtgcaga cggccttcac cgcgtcgcgg ttgatcttgc
    23821 cgcgcgaggt gcgcgggatt tcctcgacca cccaccagcg caccggcatc ttcggttcgg
    23881 cgagatgttc cttcatccac tggtgcagtg cgcggaaagt gccatcgtcc tgtctggcca
    23941 tgaccaccgc catgcccacc acttggccat agatagcgtc gtccagcgcg aaggtgcaga
    24001 cgtcgctggc gtgctcgaag cgttccacca ccgcatcgac gtcggccggg tagatcttca
    24061 tgccgccctt gttgatttca tcgcgctcgc ggccgcgcag caccagccgg ccccgcgggt
    24121 cgatcaggcc aatgtctccg gtcaggaacc agccgtccac taccgcctgt tcggtgaggt
    24181 cgtcgcgctg gaaataacct ttcatcagtg ccggcgtgtt gagccagacg aagccggatt
    24241 ctccgggggt acattcgtca tcggcgctca aggggcggga ggtatcggag gtgcgcaaca
    24301 ccttgatgac cgcgccccag ccttggccga tcagcccgtc ttccgccgga cagtccgcct
    24361 cgctcagtcc agccacccaa ctgccggttt cggtaatgcc gtaggcgtta cagacctggc
    24421 gtgtaccgct ccaacggcga atgtcttccc agaccccggc cgacagcggt gccgatccgc
    24481 agtgtatgcg ttgcagggtg cctgcctggg gcggtttgga aatcttgagc gccagcttcc
    24541 acaccgccgg cacggacgac atgaaggtga tgcgatgctc gtccagcaga gcccccaggc
    24601 gcatgatgat gtccgggcgg aacggcgggg tgataaatag gtcctgtccc gacagccagg
    24661 ggaacagaca gttgcagatc agcccatggc cgaagtgcgt tggcagcagg cacaacgttc
    24721 tggcaaaggc gtctgtgccg agggcctcgc gcagggtgat ccagcgtgcg cgcagcgagc
    24781 gatgggtatg cactacaccc ttgggggcgc cggtggagcc ggaggtgaac aggatgaggg
    24841 cgtcgtcatc cagccggctt aagcccaccg ctgcctgctg atctccggtg tcggtggtat
    24901 tgaccacgcc gacaccggct tcgtcgaggg cttgaatgac gtgggcatcc gtcgcgtcgt
    24961 ccactacggc caggcgcggc ttggccgctg cgaccagggt ttgtacctcg aacggcgtga
    25021 ggcgtgcatc gatcggcacg gcacaggcgc cgagacgcca gatggcgagc agctcggcaa
    25081 agaactccag acggttgccg aagggcagga atacccggtc gcccctggcc agcccttgtg
    25141 cctggaaacg tgcgatgcga agcgccacct gacggccgat ttcctggcgt tcccagtgcc
    25201 ggccgctgac cggctcggca agatttccaa cactcaaatc aacgaacata tctcaatata
    25261 cccttactca tgccagacca aaaaggccca aaaagaaatt cactctggac aggaaaggcg
    25321 catcgctcct gacattccag atgaagatga agctgaaaaa agtccatacc ccggccatgc
    25381 gcagcaggcg gcgggcagtg ccgggggctt gtgcttgcat gctgcggcgc tgctcccgca
    25441 ccatggatat gtagatgcct aaagccagca gcaggcagta gaacatcctc gagcggagat
    25501 tatacaaggc ttggaatacg tctccttgta gtagcaaatc gtactcttgc aggacgtggt
    25561 agtacatgtt gccaaaaagc gccgccgcca tgacggccag gaacaggcgc agctgcggcc
    25621 gattgcgcag tacgccgccg agttgcgcga aagttggcat aaagaagaag ttgaccagca
    25681 gttccttgaa ataatagtag tagcggttcc agaactccac gatcgattct gccagcagcg
    25741 gcttgtaggt gttgcggaac acgttgaaac cgaacaggcg caagtaaccg atgatctcgt
    25801 ggccgcggat ggcatgtttc agcacttgcc atacgaattc gcagtagatg ctggcccagg
    25861 aggccgcaat gggggcctgt ccacctgctt tcaccagttc acccagctgg gggatggcca
    25921 gggtgaaacc acccagcgcg acggtcagtc cgttgcctgg cccatacaac accccttcca
    25981 gcaggtcccg cacccagcgc agcaactggc acagcagcag gagcttgatg ccggcgagct
    26041 gggagcgcgc cagctcctcg ctggtcttgg cctcgaggcg cgaaaggtag accagcccct
    26101 tgccgtaggg cgtctcggaa ccgccgtagg ccggccacag ggtcagcaag tgatccgaaa
    26161 agcgcgtgcc cgccatgcgt ccgtattgac ccgccagcag cacgtagccg cagcgccaga
    26221 gcaggtaagg gaagatggcc gccacgccga gcagcaccga ccgccacagg cccgcggccg
    26281 gcgtggtcac ccacagggcg gccagcattc cccaatagaa caggtgcagg gtcagttgcg
    26341 gatgtcggcg caccatgccc ggcagcgcgg agaacctggc tgctgcgcgg tagcacagcc
    26401 agagcagggc gaagatggcg aggatgacca gcgcgatacc caggccgtct gttggcaccg
    26461 gcagctcgtc ggccttggaa aggaatttcc agggatactt cgcgccttcc cagactgcgc
    26521 cgatggccag gatcatgcga cgtcgcgaag gggtgaacca gtcgagtgct agcccaagcc
    26581 gtgcctgcca ccacgcgatg acggcggcat tttcatggaa ctgcgaaagt tggcttaatt
    26641 gcatgcggtt aaggatttat ggccggcgag cttggaggtt atagtcaaat tagggcaata
    26701 ttaccatttt ccgggcacca atttcccggc acaacactcc accggcgttc cgattacgat
    26761 gcaatcctct ctggcaatac cgacaaacaa gcgtcttgtt ccatttctgg cgatactcgg
    26821 cattctgctc gggctgtggc tggccatgct ggagaacggt cgcatcaacg gcgacgccga
    26881 tctttacctg gagatggcga gccggtttgc gcaagacgat gcgcagggtg cgctgtcgct
    26941 ctatcaatgg ccgctgcttc cctggctgat ggctcaggta cattacctca ccgggctcga
    27001 tgtcgaaagt gccggccgcc tgctgggggc gatttttttc ggcattgcga catgggggtt
    27061 cctggagttg atccgcgaag ccggcggcag ccgcaacgct attctggccg gggcgctgct
    27121 gctgttttcg tcgccctaca tcgtcggcga tgtgctgccg atgatcatgc gcgaccaggg
    27181 cttctgggct ttctacctgc ttgccctgct tttttttctg cgcttttatc gttctctcag
    27241 ccttgccgac gccatgctct ggcagctttg cgtacttgtg gccaccctgt tccgggtgga
    27301 ggcggtcagc tttatattgc tgatgccgct gctattgccg ctgcgccgag atatttcatg
    27361 gcgcagccgt ttgcaaagcc tgggcatggc ttatcttgtt ccattaatgg gcataattct
    27421 ggtcatcctg ggcggcctgc tgttgccggc agccgagttg cagcaaaaac tcgggcggtt
    27481 ggcggaagta cagagttcgt tgcaaagcgt ctaccaccag atttccgggg ggctcgcggc
    27541 caaggcccgc atctatggcg accaggtgct gggtaagtac atggacaatt atgcgatgct
    27601 gggcttgtgt cttaccctgt tcagcgtaat cgcaggcaaa atcatcggcg ccagcggctg
    27661 gctcgggatt gtgctggcga cgaccggcaa gcgatgccag gcccctgcca tggacccgga
    27721 tgcccgcact gtgctgctct gggctgccgg tatcaacctg ctcaacctgg cagtcatttt
    27781 actgaccgcc tatttgcttt cggcgcgcta tgccgttggc atgggattca ttgtgctggt
    27841 gttcgccgcg ttcaatctgg gcggcctgtg cgacaggtgg ctggaaagcg gcgcccgccg
    27901 ttcccgctgg ctggccttgg gcttgctgct cgccgccttg ctgatggcat accatctggt
    27961 caggagcctt gccccgcatg acgccgacta caactacgaa caagccgcgg tggcatgggt
    28021 caagcagcac gccgccaaag atgcccgcat ctattacgac ggcgcccggc tacgctatta
    28081 cgcccatgcg ccctggggcg gcagggcgga cgaatgggaa aacaacctac ccgcgttgca
    28141 ggccaccccg gcccgattcg attacctgct actgacggtc aagggcaaga accccaatca
    28201 gttggcgctg ctggaacaat tggcgcagta ccgcaaggtg caggaattcc gtcgcgacaa
    28261 cagcaatgcc atcgtggtca tggtgtcccg ggaattgccg gactgaatca agccgtctag
    28321 gccttggtga ccagcacgtc ttccatgatc aggtattcca gatcggaacc gaagaacatg
    28381 ttgagtgcgt caagcgggct gcagatcatg ggttcgccgc ggcggttgag cgaggtgttg
    28441 agcaccaccg ggatgccgcg tagcctctcc agttcctcga tcaggcggta gtagcgcggg
    28501 ttggtgtcca tgctgactac ctgcgcgcgc gcggtgccgt cctcgtgtac cacttccggg
    28561 atgcgagatt tccagtgctg cgccacatcg aaggtaaagg tcatataggg cgcaggatga
    28621 tcggtgccaa ggatgtccgc cgccgcgcgg tccagcatgg aaggacagaa cggccgccag
    28681 cgttcgcggt acttgatctg cgcgttgatg cggtcggcta cgccggggtg gctggggtcg
    28741 ccgaggatgc tgcggcagcc cagcgcgcgc gggccgaact ccatgcggcc ctggaaccag
    28801 gccagcgggt tgccggcggc gaggatttcg gcgcccttca cggaagcatc cccgatgcgt
    28861 tgccacttcg gcttttccgg gtgcgcctcg caggcggcga tgcattcctc agtggtgtag
    28921 gacggtccca gatacgcgtg cttcatcggc tccacggtat cgcccttttc agcggcgacg
    28981 taggtggccg cgcccaatgc ggtgccggcg tcgccggagg ccggctgcac gaacagctcc
    29041 ttcacgcccg gcagagcaat gatgcgctgg ttgagcttga cgttgagcgc caccccgccg
    29101 gccatggcga tgcggccggt ctcgcggatg atgtcgccga ggtagtggtc gatcagcttg
    29161 agcgccacgt cttccaataa ctgctgcacg gcggcggcgt aatggatgta gggatggtcg
    29221 atctcgtcgc cgtcgcgctt ggggcccagc cagtcaacca gtgtcgggct gaagtagaag
    29281 cccttgccgt tttccttgta gcggcgcagg ccgaccacat tcaccagcct ggtgttgacg
    29341 cggaattcct tgccgtcgaa ttccagcagg cgcgacaggt cgtatttgtg cggatcgcca
    29401 tagggcgcca tgcccatcac cttgaattca ccgtccagca tctcgaagcc gaggtattcg
    29461 gtcatcgcgc catacaggcc gcccagcgaa tcggggtcgt agaactcctt gatcttgtgg
    29521 atcttgccgt tttcgccgta gccgaagaag gtggtggcgt actcgccctt gccatcgatg
    29581 ccgacaatgg cggttttttc cttgaagccg ctgaggtggt aggcactgct ggcgtgggcc
    29641 agatggtgtt ccaccggcac gaatctggca ttggagaggc ccagtccctg catcagttcc
    29701 agtgccttgt cgcgattgcg gcggaaacgg cggttgccgt tgaaaatggc atccagtgcg
    29761 cggtccggcg cataccagtg gcgcatggcg tagtgccagc gcgccggcgt ggagagcggg
    29821 atttcggcgt aggggaaggc caccacatcc acctgctccg ggctgatgcc ggcttccttc
    29881 aggcagtact tggttgcctc gtagggaaac ttgcccttgg cgtgcttgtc gcgcaggaaa
    29941 cgctcttcct ccgctgccgc gatcagttta ccgtccacca gaatggcggc cgaggggtcg
    30001 tgccccacgg caccggataa tcccagaata atcatgtgtt ctcgctcttt ttcataccga
    30061 cagcaactct cgcagccatt tatcgttttg gtaaacctgt tccagggctt gtcgcatcat
    30121 ttcggcggtc agtggatcgt tccgccagtt ttccatccag cgactcaagt cgcgcccatg
    30181 cccacgacgc agtatccatt tgcagcgaac cgcgcgcatg gcatccagat ccagcagcag
    30241 cggctgctct tctgccccga tcagaacgtt ggtcgctttc atgtcgccgt gcaccaaccc
    30301 caggcaccac aacttgtgca gcaggcggga gatgttctga actgcgtact gcttgcgctc
    30361 cgccgaaact gcaggatcag cgaagaatgc cgtcgcatcc gtcgcatcca catattcggc
    30421 aacataatac gcctgccgcc gcaacgaccc ccagcgacgc tccagaagtg ccagtggctg
    30481 cgccgtcggg atgccgagga tgttcatccg gtatgcattc gcccaagacg ctgcggcacg
    30541 cgaaggccgc caggcacgcc ccaagccatg ccagaaattt ttgatattgt agcgcttgac
    30601 cacgatgttg cggtctttca gcggcaatgc cagtacggta caggtgttgc cgtttttcaa
    30661 tattcggacg ttcgcctctc gcaacctggc ttccgggtta tccagcaggg cccgcaactc
    30721 ctcgctatct tgtggccgcg cgatggcttt gtagctgttc cagcggtgct cgatgataac
    30781 gtcagtgcaa gtgcggaaca ccttgtggtc ggcgtagctg cgtgcgacac gcagacgatg
    30841 tgccatcacg cgtgccggga atgtccgtat ttcggctggc cgcaatgcca tgccgagtgc
    30901 ggcggcataa tgctgctgta cttgcggcag ccaggggtcg tccacagcat cgaacttgga
    30961 cagcagcagt gcaaggttgt ccagcgcggt acggcggcct agcggggctg cgtggcagcg
    31021 aatgccgtcg ccatccaggg tgaatacagc ggcctcggtc accaggaaat tcttcaggta
    31081 caggtcgtgc tgtatcaatc cggacaggtg gtgcttcgct acggtttgta ccagcctttt
    31141 caccagatcc agccgcgccg ccgggtcgtg tgccagcgtg cgccacacca cctcggcgct
    31201 gctgcccgtg acctcggcaa acaccagcac ccggccgccg ccgttggcga tgttgccatc
    31261 gaacagcagc tcgggcgtcg cgatgcccgc ttcagtcagc gcatgcaccc cggatgcatc
    31321 gcgtccggca tcgcgtccgg ccttgctgcc gaagaacagt ttggcgtata ccggcctgcc
    31381 atcccagctg ccgcgacata ccagccggcg ccccggaacc aggcgcacga tggcctcgca
    31441 ctgcaactgg ggggcgccag agaggtcgac gcgatagctg ccggaagcag acgcgatatt
    31501 ttcgatcagg ttgtccgggc ggggcacggg agatgggcct attggttagg cgagcgcgaa
    31561 ttatactgtt tcggccgctg cgagcgtttg agtatcaggc ggcataacag cttatgccag
    31621 gggcttagcc gttcgatgcc caggtagtgc ttgaagaaat acattcgggc tgtcttgctc
    31681 caggcgcggg catgacggtt gagcgtcgcc aggtcataga tcgtgcgcgg agtcgcaagc
    31741 agtgtcgccc tggacttttc cagatcgatc acggctacca ggggatcacc tgctgctttc
    31801 cggttgacga acagatgctt gggatacagc gagcggtggt gaatgccggc atcgtgcatt
    31861 ttccggacca tggcggctac gccgcgcaac agcgcgcgtt gttcggacag gggcgggcgt
    31921 ttgtcatcgg caaataaatc ctcggtgacg tcttccagcg aacggaagcc cgccagttcc
    31981 tcgctgacca ggatggcccg cgcggcacgc tggccgaaaa ataccggcac tggcgcgggc
    32041 acgccgcgtg actgcaggaa gcgcagcatg cggaattcgc gggcgaacgt cggttccccg
    32101 cgcagggggt ggcgcagggt ggggcgctga tgattttcct ggcgcttgag aaaaacgcca
    32161 gcctcgccac cgtccggcaa gcgcaggact acacgaccgg cgccactcca gccgccgcgg
    32221 cgattgttgg gttcctcgaa ccactcggcc tgataagacc aaagctggtc gaaactggcc
    32281 aaatggttgg attccagcaa ctgccggtaa gcggggtaga taaaatcgga caatggaaat
    32341 cagtaatggc ttgaaaagat agcaggaatt ttgcc
//
LOCUS       DAOMFA010000002        27585 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_73_length_27585_cov_4.936397, whole genome shotgun sequence.
ACCESSION   DAOMFA010000002 DAOMFA010000000
VERSION     DAOMFA010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 27585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27585
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73_length_27585_cov_4.936397"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /locus_tag="PKW44_00165"
     CDS             <1..291
                     /locus_tag="PKW44_00165"
                     /inference="COORDINATES: protein motif:HMM:NF039992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SxtJ family membrane protein"
                     /protein_id="HQR50033.1"
                     /translation="LWALELSGGFLLLALVIPRVLAPANRAWTKFGLLLHDIVSPIAL
                     GILFFVVVTPTGLLMRLLGKDPLRLRFERSAKSYWIVRTPPGPDAESLKNQF"
     gene            303..452
                     /locus_tag="PKW44_00170"
     CDS             303..452
                     /locus_tag="PKW44_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008061208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5989 family protein"
                     /protein_id="HQR50034.1"
                     /translation="MSFLKELWAFMRSRKKYWLLPILVMMVVLGVLIVMAHGTAVAPF
                     IYTLF"
     gene            460..2292
                     /locus_tag="PKW44_00175"
     CDS             460..2292
                     /locus_tag="PKW44_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018230615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase"
                     /protein_id="HQR50035.1"
                     /translation="MYVLGISAYYHDSAAALLCDGEIIAAAQEERFTRKKHDAGFPKN
                     AVEYCLKEAGIGLGEVDYVAFYDKPFLKFERLLETYVAFAPRGFQSFRMAIPVWLREK
                     LFLKDLLRKELKAYAPDYDWDSRLRFSEHHLSHAASAFFPSPFEEAAVLTMDGVGEWA
                     TTSLAIGRANKLEVVKEIHFPHSLGLLYSAFTYYTGFKVNSGEYKVMGLAPYGEPKYV
                     QQIFDHLVDLKDDGSFRLNQSYFNYCTGLTMTSPKFHDLFGGPPRKADELLTQRHMDL
                     AASVQAATEQIVLRLARAIRQETGLSNLCLAGGVALNCVANGKVLRDGQFDGLWIQPA
                     AGDAGGALGAALAIHHIHLGQPRTADGRDKMRGSYLGPAFEQADIERRLTAAGAKFSV
                     LNDSELPQTVAKALADGQAIGWFQGRMEFGPRALGGRSVLGDARSPAMQSVLNLKVKY
                     RESFRPFAPSVLRDDVADWFELDGDSPYMLLVADVVKNRRRVMTEAEQALFGIDKLNV
                     PRSEIPAVTHVDYSARIQTVHAETNPRYHALIAAFKQQTGCPVIVNTSFNVRGEPIVC
                     TPEDAFRCFMGTEIEVLAVGNCFLRKEDQDPALKLNYETAFELD"
     gene            2279..3502
                     /locus_tag="PKW44_00180"
     CDS             2279..3502
                     /locus_tag="PKW44_00180"
                     /inference="COORDINATES: protein motif:HMM:NF028132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR50036.1"
                     /translation="MNWISTISKNFSTWRGGAMYFAALLISLHALLFYGLPLAGILFA
                     WLLLAVWFMFAGGAQALLTAVPVAIFTLLLNISIQLSGLERSMYYRPHELLKSSSPDF
                     GDVYQPNTRFSMHMPFGDIEAMEKVGIQEPREIEYQTDALGFRNPTNYHGQKFVLVGD
                     SFVAGLADTQSCITTEWLRREHHIDTYNLGYPGDMDDYVNRVRAFRSRYGKNFHVLLF
                     FFEGNDFIPYSAKPPRSSSLLQRYYSWFKNSAIWRYTRALYLRGTQRHAGKNENASLV
                     RDVGHHPIAFYSQDLVAVANRQALLDSQLRFTQALQALQPNLAQIFFVPSKYRVYARW
                     LDPKPLPNEQWLYLERTAKQTGIPVHNLTPALEKEAERLLPQGQYVYWRDDTHWNCNG
                     MRTAATEVAHVLSQD"
     gene            3504..4544
                     /gene="waaF"
                     /locus_tag="PKW44_00185"
     CDS             3504..4544
                     /gene="waaF"
                     /locus_tag="PKW44_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HQR50037.1"
                     /translation="MPSPSHIDFSRILILGPSWIGDMVLSQSLFRAIKARNPEARIDV
                     AAPAWTLPLLERMPEVDATISLPFRHGQLALGERFRFGKQLRGRGYIQAILLTNSLKS
                     AILPFAAAIPRRTGFLGEFRYGLLNDIRPLDKQALPRTVDRFAALGLEPGEQQPLSLP
                     LPRLAANPEQALLTLRRLGHAAPEVPVLGLCPGAEYGPAKRWPATYYAEVANTALVRG
                     WQVWLFGSEKDVQVTGEINTLTGRRCLDLGGRTTLGEAIDLMALTTEVVSNDSGLMHV
                     AAALGKPLVAVYGSSDPHHTPPMSEKASIAYLGLPCSPCFKRECPLGHLNCLKQLAPH
                     EIIEKLKLDVRV"
     gene            complement(4623..5759)
                     /locus_tag="PKW44_00190"
     CDS             complement(4623..5759)
                     /locus_tag="PKW44_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HQR50038.1"
                     /translation="MEQGQTEKFIFDLLRLMLVKNASDLFITVGFPPALKVDGKITPL
                     SNQTLTMQQCKEVCRSIMNDKQALEFDSTNECNFAISLPGMSRFRVNAFVQRGSPGLV
                     FRTITNKIPKFEELGLPEVLKDITMTKRGLVIFVGGTGSGKTTSLAAMVGHRNENSYG
                     HIITIEDPIEFVHEHKNCIITQREVGVDTDNWYVALKNTLRQAPDVILIGEIRDRETM
                     DYAIAFAETGHLCLATLHANSTNQALDRIINFFPEERRPQLLMDLSLNAKAFISQRLI
                     PKVEGKGRIPAVEIMLNSPLISDLIFKGDVHEIKEVMARSRELGMQTFDQSLFDLHEN
                     DHISYEDALRNADSVNDIRLRIKLEGKAGKDKDLSAGLKDMGIV"
     gene            complement(5793..6839)
                     /locus_tag="PKW44_00195"
     CDS             complement(5793..6839)
                     /locus_tag="PKW44_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019916355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HQR50039.1"
                     /translation="MDISDLLAFSVKNKASDLHLSAGMPPMIRVHGDVRKINVPSMDH
                     TEVHDMVYDIMSDGQRKIYEENLECDFSFEIPNLARFRVNAFNHNRGSGAVFRTIPSK
                     ILTLEELNCPEIFKEIAATPRGIVLVTGPTGSGKSTTLAAMVDYINDNDYGHILTVED
                     PIEFVHQSKRCLINQREVGPHTLSFANALRSALREDPDVILVGEMRDLETIRLALTAA
                     ETGHLVFGTLHTSSAAKTVDRIVDVFPAAEKEMVRSMLSESLRAVISQTLLKTKDGSG
                     RVAAHEIMIGTPAIRNLIRENKIAQMYSSIQTGQNVGMQTLDQNLQELVRRNMISLAE
                     ARGKAQNKDTFVGS"
     gene            6886..7593
                     /locus_tag="PKW44_00200"
     CDS             6886..7593
                     /locus_tag="PKW44_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HQR50040.1"
                     /translation="MTSISERLQAIQTYIRRAEQQAGREEGSVQLLAVSKAQPVSAIW
                     EAFQSGQRLFGENYLQEALEKMAELADLPLEWHFIGPVQSNKTRPIAENFAWVHGVDR
                     LKIAERLSEARPPDLPPLHICLQINVSGEASKGGIAPQEAGALAQAVSSLPKLKLRGL
                     MAIPAPTTDVAMQHEQFRMLRETLESLNGQGLSLDTLSMGMSHDFPVAIAEGASIVRV
                     GTAIFGDRPAKELRETS"
     gene            7590..8399
                     /gene="proC"
                     /locus_tag="PKW44_00205"
     CDS             7590..8399
                     /gene="proC"
                     /locus_tag="PKW44_00205"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HQR50041.1"
                     /translation="MKISFIGGGNMARAIIIGLLKRGWDAASINIVEPDAGKRSALTT
                     EFGVTVSDQLPSVAHSEVIVLATKPQQLRDAAIFLGSLLDRQLVISIAAGIRCADLIR
                     WLGNYGAVVRVMPNTPAQVQAGISALYAAEGVNATQQQQAESIMGAVGATLWLEDEMQ
                     MDAVTALSGSGPAYVFYFLEAMQQAGVDLGLTPGQAGALALQTFLGAARLAEGSGQDF
                     ATLRSQVISKGGTTERALNSLENAGVKAAIIRAIDAAAKRSRELGDLLGKD"
     gene            8399..8980
                     /locus_tag="PKW44_00210"
     CDS             8399..8980
                     /locus_tag="PKW44_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HQR50042.1"
                     /translation="MSALAQAAAFLLNTLFGLFTLALLLRLYLQLVNAPFHNPFSQAV
                     VTLTNFAVRPARRIIPSWGWLDTASLLLAYASQLLLQLGILWLNDFPLLVAGAQVYIA
                     LFALAALGILKLSIYIFLYAVLLLAVLSWVNPYTPLSPVLDSLTRPLLRPLRKFITKP
                     GAMDLTPLIIFIIAELLLMLLVAPLEQQLLKLF"
     gene            9183..11162
                     /locus_tag="PKW44_00215"
     CDS             9183..11162
                     /locus_tag="PKW44_00215"
                     /inference="COORDINATES: protein motif:HMM:NF012570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HQR50043.1"
                     /translation="MSNSLEQQITDFHHWRDQIAGAIDNYREWRNDTGNTDAIEELRL
                     YDLAEALRRDRLVLAFVAEFSRGKTETINALFFSDYKQRLLPCEAGRTTMCPTEIYWD
                     ANEEPYIKLLPIETRKSDEGLADLKKSPKEWSKLRLDPSSATATQEAFQVLSQQKQVS
                     LEEARELGLWDDNDPVLVHTLQTLGKVEVPVWRHALINFPHPLLQSGLVILDTPGLNA
                     LGTEPDLTLSIIPNAHAVIFLLATDTGVTKSDMQIWNDYLRDKTSRKLAVLNKIDILW
                     DDLKTEAEIQAMIQSQVESSARHLGLAISDVFAISAQKALLARINGDSTLLKRSGIEQ
                     VEQVLAQELVAAKHVILRQTVVGEAGNMMKVSRRAIQLELTGVRSRLQELDALRGKNQ
                     DVVMGLMTEAAADRKVYEATVRTFVQGNQKVMQMGEALMHQLSLRKLDEMLEQSRRQI
                     GDSWTTHGLNQGMKMLIRQTIAMAADITKQGQEIKAIGDDLYQLFHVKHGFEMRNPPI
                     LDMSHFHQSMMALQQTTDEFCNDPVNVMTEKHFLVRKFFFSLANLVRTHFEQAHNESK
                     SWLKELLAPLAQQVNQHKSQLDKRTESLMTIHANLKSLQSNVNALMNQESRLQNQSTA
                     LDHILLKLMKAAQNSSNPAPQPAGAAERQAPGVPA"
     gene            complement(11197..12645)
                     /gene="rng"
                     /locus_tag="PKW44_00220"
     CDS             complement(11197..12645)
                     /gene="rng"
                     /locus_tag="PKW44_00220"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HQR50044.1"
                     /translation="MSEEILINVTPQETRVAAMEQGAVQELLLERAGSLGLVGNVYKG
                     VVCRVLPGMQSAFVEIGLARAAFLHVADILEYDTNTEKPIQELLHEGQTLMVQVIKEP
                     IGSKGARLTTQISIAGRFLVYLPQQEHIGVSQRIEDEAEREALRERLLKLLPPDNRGG
                     YIIRTMAETAEDDELRADIDYLHKVWADIQTSGCDAPARSLVFQDLTLPLRVLRDMVG
                     GHTDRILVDSRETWQKMLTFSEHYAGCVLSRIEHYSGERPLFDLYGIEQEIEKALSRK
                     VELKSGGYLIFDQTEALTTVDVNTGGFVGGRTLADTIFKTNLEAAQSIARQLRLRNLG
                     GIIIVDFIDMEEEGHREAVLEELRRALEKDRVRVGIHGFSGLGLVELTRKRTRESLAH
                     ILCEPCPCCQARGEIKTAQTVCYEILRELVREARQFNAREFRVLASQQVIEMFLDEES
                     QSLAQLSDFIGKPISLQVETQYGQETFDIVLM"
     gene            complement(12642..13235)
                     /locus_tag="PKW44_00225"
     CDS             complement(12642..13235)
                     /locus_tag="PKW44_00225"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HQR50045.1"
                     /translation="MLKPPHIYLASRSPRRGELLEQLGVSFEVLPSDVDESVLPDESP
                     EHYVLRLAREKALVCVQRLAVQGLPPRPVLAADTTVCIDGMVLGKPEHDVDAAAMLRR
                     MSDRWHAVHTAIALAHDGRVEVALSSTQVEVALLGEDEIAAYVASGEPRDKAGAYGIQ
                     GLAGAFIRRIEGSYSGVMGLPVYETAQLLKKFGVKLL"
     gene            complement(13229..13777)
                     /locus_tag="PKW44_00230"
     CDS             complement(13229..13777)
                     /locus_tag="PKW44_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HQR50046.1"
                     /translation="MATVLIPLAQGCEELEAVTVIDILRRAGIEVITAGLLPGPVVAS
                     RGTVLLPDAELGQVLHQAFDMIVLPGGMPGSEHLKNDARVIALMKQLAASGKYVAAIC
                     AAPMALHAAGLLQGKNATSFPGVLDQLPGSHHYCSDAVVVDGNIVTSRGPGTAMDFAL
                     TLVELLVGKASRERVESGLERC"
     gene            13925..14368
                     /locus_tag="PKW44_00235"
     CDS             13925..14368
                     /locus_tag="PKW44_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HQR50047.1"
                     /translation="MIWKPNVTVAAIIECDGKFLLVEEETADGVMFNQPAGHLENGES
                     LLDGVAREAWEETSHRFQPTGLLGVYHWKHPKKDITYLRFAFIGNVTDHDPSHPLDAG
                     ILRAVWMTPDEIRATQDRHRSPQVLTCIEHYMAGRCFPLSVLTHL"
     gene            14365..15441
                     /gene="mnmA"
                     /locus_tag="PKW44_00240"
     CDS             14365..15441
                     /gene="mnmA"
                     /locus_tag="PKW44_00240"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HQR50048.1"
                     /translation="MKKQRVVVGMSGGVDSSVTALLLKQQGYEVVGLFMKNWEDDDSE
                     DFCPAKQDLIDAVSVADRIGIDIEAVNFSKEYKDKVFANFLQEYRAGRTPNPDILCNS
                     EIKFRAFLDHAMSMGADKIATGHYAQVREAASLFQLLKAEDGSKDQSYFLYRLNQEQL
                     SKTLFPLGKYLKREVREMARKAGLATSEKKDSTGICFIGERPFREFLQRYMPTEPGDM
                     HTPEGKLVGRHHGLMYYTLGQRQGLGIGGSREGDGEPWYVAAKDMARNELVVVQGHDH
                     PLLLSDRLNAGQMHWISGSAPRTHWVYAAKTRYRQADAPCEIDAIDGDRCEIRFGDSQ
                     WAVTPGQSVVVYESNVCLGGGIIE"
     gene            complement(15431..16321)
                     /gene="nhaR"
                     /locus_tag="PKW44_00245"
     CDS             complement(15431..16321)
                     /gene="nhaR"
                     /locus_tag="PKW44_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HQR50049.1"
                     /translation="MNFKHLHYFWTVARSGSVARASERQHLTPQTISAQIKLLEQGLD
                     ATLFRPAGRGLELTEAGRLALSYADQIFALGDELEAALASRRGVLALPFRVGITDVVP
                     KALAYRLLAPARHLAEPLRLICREGKLDRLLTELSLHKLDLVLADRPIPESMGVRGLS
                     RKLGESALAFFAAPALAESCGEFPRCLDDAPLFMPGDDATVRGRIERWLERLRIAPRV
                     VGEFDDGALMKAFGQEGGGFFPSPAVLADEIEVQYGVRLVGRVEEVREEFYALMPDRS
                     FAHPGVRAITEAAHAGMFIR"
     gene            16467..17357
                     /gene="htpX"
                     /locus_tag="PKW44_00250"
     CDS             16467..17357
                     /gene="htpX"
                     /locus_tag="PKW44_00250"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HQR50050.1"
                     /translation="MKRIMLFLATNLAIVLVLSVTMRILGVEPYLNANGLNLGALLTF
                     AAVMGFGGSLISLAISKWTAKMSVGAQVITSPQNAQERWLVETVERQAQAAGIRTPEV
                     AIYESPDVNAFATGMSKNSSLVAVSTGLLQKMTRQEAEAVLGHEVSHAANGDMVTLAL
                     IQGVVNTFVMFLSRVVGYLVDKVVFKTERGHGPAFFVTMIIAELILGILASIIVMWFS
                     RQREFRADRGGANLAGRQGMINALKRLQSLQEPQPLPDKMAAFGINGGRSGLMRLFTT
                     HPPLEERIAALEAADDLAGH"
     gene            17357..18328
                     /locus_tag="PKW44_00255"
     CDS             17357..18328
                     /locus_tag="PKW44_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019896755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HQR50051.1"
                     /translation="MELSSVVTPAFWAGFILFVLAMLALDLYVLGGKNAHKVSAREAL
                     GWTLVWVTLALTFNGLLWWWLDGTLGGEIANEKALEFLTGYLIELSLSVDNIFVFIMI
                     FTYFAVPAEYQRRVLIYGVVGAIVLRASMILAGTLLVAKFHWILYVFGAFLVITGFKM
                     LIFADKEADLESNPVLRWMRGHLRVTRDYHGEKFAVIKDGVRWFTPLFLVLILIETSD
                     VIFAVDSIPAIFAVTTDPFIVFTSNIFAIMGLRALYFLLVDMADRFHLLKYGLALVLV
                     FVGTKMLIADFYKVPIGMALGAVAILLLSTVAASLIATRQSRVKSPH"
     gene            18349..18888
                     /locus_tag="PKW44_00260"
     CDS             18349..18888
                     /locus_tag="PKW44_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013149321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HQR50052.1"
                     /translation="MKVIESKLENFIFNSRWLLAPFYIGLVLALGLLLVMFGREFIHF
                     VPLALHGAEKDVIIGILSLIDVVMVANLLIIIVFAGYENFVSQIETGDHVDRPSWMGH
                     VGFSELKMKLIGSIVAISGIELLRTFMHLTGTATTELSFGEWNALLAWKVGIHLTFVV
                     TGVLFALSDRIGSDKNGGH"
     gene            complement(18928..19542)
                     /locus_tag="PKW44_00265"
     CDS             complement(18928..19542)
                     /locus_tag="PKW44_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HQR50053.1"
                     /translation="MKLFMTPNSPYARKVRVMLAEKHIECEMVEVPSLAAPDSPVPAY
                     NPLGKVPTLVLDDGTSLYDSVVIVDYLDHKTPVARLIPQDNNHRAVVRRWEALADGIC
                     DAAVAVVMEGRRAPERQDETVIERQALKVQRGLATLAEGLGDGKWVMGERFTLADIAV
                     GVMLGYVALRMPEINWREQYPNLDRLYQQLLSRPSFKDTVPQAA"
     gene            19590..20429
                     /gene="nadC"
                     /locus_tag="PKW44_00270"
     CDS             19590..20429
                     /gene="nadC"
                     /locus_tag="PKW44_00270"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HQR50054.1"
                     /translation="MQISPADIERSVRFALEEDIGGGDLTALLIPPGQEATACVISRD
                     SAVLCGRAWFDACFRLLDNGTRIHWLATEGSTISPNQTLCEIRGTARSLLTAERSALN
                     FLQTLSAVATLTRRYVDAVAGTGAAIMDTRKTLPGLRLAQKYAVTVDGGKNQRIGLYD
                     GVLIKENHIAAAGGIQQALAAAHQASGDTPVQIEVETLEQLQTALDAGATLVLLDNFT
                     HDQMRTAVVCNAGRAQLEASGNITLDNVRAVAETGVHRISIGALTKNIQAIDLSMRFE
                     VGA"
     gene            20442..22220
                     /locus_tag="PKW44_00275"
     CDS             20442..22220
                     /locus_tag="PKW44_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HQR50055.1"
                     /translation="MKPDQPPAAKSHKLTLGEVLRLMVHDGMVPKDIAEQLFKDRQLD
                     SSNLHPLAVIAYQQWKNPRSPGKPFTLEFLTEWLANRIGVPYFHIDPLKLNFSAIAGI
                     VSKNYAERLRIMPVKVSEHEVVIATAEPFLNDWVADLERILSLKIQLVMANPLEINRY
                     LPEIYNLASSVQMADDAKAGQVVGVQNLEQLIELGKNKSLDANEHHVVTIVDWLFEYA
                     FSQRASDIHLEPRRDVGNVRFRIDGVLHQVYQLPPNITNAITSRIKLLGRMDMVEKRR
                     PQDGRIKTVRADGTEIELRLSTMPTAFGEKLVMRIFDPQILVRDFTSLGFSQEQLTTW
                     NNWTSVSNGIILVTGPTGSGKTTTLYSTLKQLASPEVNVSTVEDPIEMIEPAFNQMQV
                     QANIGLSFADGIRTLLRQDPDIIMVGEIRDIETAEMAIQAALTGHLVLSTLHTNDAPS
                     AITRLLELGVPAYLINSTLVGVMAQRLTRTLCPQCKEAGEIREEAWQDLVHPWKVSKP
                     AQVSRAVGCLDCRMTGYLGRTGLYELLPISMEMRRLITKDADLGKIKTQAHREGVKSL
                     RFNGAEKIAAGMTTFEEVIKVAPPGD"
     gene            22327..24039
                     /locus_tag="PKW44_00280"
     CDS             22327..24039
                     /locus_tag="PKW44_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 catalytic subunit"
                     /protein_id="HQR50056.1"
                     /translation="MQLTGAEIVIRCLQEENVKFVFGYPGGAVLNIYDEIFKQDKFKH
                     VLVRHEQAAVHAADAFARSTGEVGVALVTSGPGATNAVTGIATAYMDSIPMVVISGQV
                     PTPAIGQDAFQECDMVGITRPCVKHNFLVKDVKDLATTIKKAFYIAASGRPGPVVVDV
                     PKDITQHTTKFEYPKSVHMRSYNPVLKGHSGQIKKAIKLLLEAKRPMIYTGGGVVLGD
                     ASELLTRLVRKLGYPMTNTLMGLGGYPATDPQCLGMLGMHGTLEANMAMQECDVILAV
                     GARFDDRVIGNPEHFFQNSRTIIHIDVDPSSISKRVKVDVPIVGDVKNVLAEMLELLD
                     LDDRAPNENTLKAWWQKIGEWRGRNCLNYDRNSTIIKPQYVVEKLYEVTKGDAFVTSD
                     VGQHQMWAAQYYKFDKPRRWINSGGLGTMGVGLPYAMGVQFAHPNAQVACITGEGSIQ
                     MNIQELSTCKQFHLPVKIILLNNRYLGMVRQWQQFFYGNRYSESYMDSLPDFVKLAES
                     YGHVGMRVEQPGDVEGALQEAFGAKLKEKLVFLDFVTDQKENVFPMVPAGKGLSELIM
                     AEEL"
     gene            24040..24531
                     /gene="ilvN"
                     /locus_tag="PKW44_00285"
     CDS             24040..24531
                     /gene="ilvN"
                     /locus_tag="PKW44_00285"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HQR50057.1"
                     /translation="MRHIISLLMENESGALSRVAGLFSARAYNIESLTVAPTEDATLS
                     RMTIVTSGSDEVIEQIIKQLNKLVDVVKVLDLNDGRYLERELMLVKVKATGAHREEMK
                     RMCDIFRGRIIDVADGSYTIELTGSGSKLDAFLEAIDKTAILETVRTGASGIGRGDRV
                     LKV"
     gene            24557..25573
                     /gene="ilvC"
                     /locus_tag="PKW44_00290"
     CDS             24557..25573
                     /gene="ilvC"
                     /locus_tag="PKW44_00290"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HQR50058.1"
                     /translation="MKVYYDKDADLSLIKKLKVTIVGYGSQGHAHAQNLKDSGAKVTV
                     ALRKGGASWSKAEKAGHKVEEIGKAVKGADVIMILLPDETMPAVYYADVEPNLKKGAV
                     LAFAHGFNVHYNQIVPRADLDVIMVAPKGPGHTVRSEYLKGGGVPSLIAVYQDKSGKA
                     KDIALSYAAANGGTKGGVIETNFREETETDLFGEQAVLCGGAVELVKAGFETLVEAGY
                     APEMAYFECLHELKLIVDLMYEGGIANMNYSISNNAEYGEYVTGPKVINDESRWAMKE
                     ALENIQNGEYAKRFILEGKTNYPEMTARRRLNAAHPIEQVGGQLRAMMPWIAKNKLVD
                     QSKN"
     gene            25645..26283
                     /locus_tag="PKW44_00295"
     CDS             25645..26283
                     /locus_tag="PKW44_00295"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HQR50059.1"
                     /translation="MNNYPHPIIAREGWPFLAGSLALSLAATFWCAMWSIPLWIISLF
                     VLQFFRDPARSAPSQTNAVISAADGKVIVVEKAWDEYLQRDALKISVFMNVFNVHSNR
                     SPIDGRVEKRWYNPGKFFNAALAKASAENERNALWLKTNDGQDITCVQIAGLIARRIL
                     CYVEPSASLYRGQRYGFIRFGSRVDIYLPLTAKPKVSLGEKVRAGETILAEI"
     gene            complement(26341..26862)
                     /locus_tag="PKW44_00300"
     CDS             complement(26341..26862)
                     /locus_tag="PKW44_00300"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HQR50060.1"
                     /translation="MIDRDGYRPNVGIILCNAHNKVFWGKRIREHSWQFPQGGIKHGE
                     TPEQAMYRELTEEVGLLPEHVRILGRTRDWLHYNVPTHWVKREWRGSYKGQKQIWFLL
                     RLVGRDSDVSLRASEHPEFDAWRWSDYWVPLDSVIEFKREVYRLALNELVTHLGADSH
                     TGRKAGGKALRAS"
     gene            27066..>27585
                     /locus_tag="PKW44_00305"
     CDS             27066..>27585
                     /locus_tag="PKW44_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HQR50061.1"
                     /translation="MIPRLYLAVLASLLVWLPLSSWGMPYAYITNQGSDNVSVIDLAT
                     DKVIATIPVGKGPVGVAVSGIGQRAYISNVDSKSVSVIDTMQNQVLEEIPLDSAPVGL
                     ALSPDGATLYIADWFEDRILAIDTANPGLRRVIASGKAPAGMAVSNDGKRLFVANRDS
                     NQVGVIDTASLQT"
BASE COUNT         5857 a         8501 c         7744 g         5483 t
ORIGIN      
        1 ctgtgggctc tggaactgtc aggcggtttt ttgctgctgg cgttggttat tccccgcgtg
       61 ctggcgccag cgaaccgcgc atggaccaag ttcggcctgc tgctgcatga catcgtcagc
      121 ccgattgcac ttggcatcct gtttttcgtt gtcgttactc cgaccggcct actcatgcgg
      181 ctgctgggca aagacccctt gcgcctgcgc tttgaacgtt ctgccaaaag ttactggatc
      241 gtccgcacgc cacccgggcc ggacgccgaa tcccttaaaa atcagttcta ggaatccttc
      301 atatgtcgtt tctcaaggaa ctttgggcct tcatgcgcag ccgcaagaaa tactggctgc
      361 tgcccatcct cgtcatgatg gtagtgctgg gtgtgctgat cgtcatggcg cacggcactg
      421 cggtggcgcc gttcatctac acgctgttct aggcctggta tgtacgtact cggcatttcc
      481 gcctattacc acgacagtgc cgccgccctg ttgtgcgacg gggaaatcat cgccgcggcg
      541 caggaagaac gcttcacccg caaaaaacat gacgccgggt tccccaagaa tgccgtcgag
      601 tactgcctga aggaagctgg catcggcctt ggcgaggtgg attatgtcgc tttctacgac
      661 aagccattcc tcaagttcga gcgcctgctg gaaacctatg tcgccttcgc gccgcgcggc
      721 ttccagtcgt tccgcatggc gatcccggtg tggctgcgcg agaagctgtt cctcaaagac
      781 ctgctgcgca aggaactcaa ggcctacgcc cccgattacg actgggacag ccgcctgcgc
      841 ttctccgaac accacctgag ccacgccgcc agcgccttct tcccgtcgcc attcgaggaa
      901 gccgcggtgc tgaccatgga cggcgtcggc gaatgggcca ccacttcact cgccatcggg
      961 cgcgccaaca agctggaggt tgtcaaggaa atccactttc cccactccct cggcctgctc
     1021 tactcggcct tcacctatta caccggcttc aaggttaatt caggtgaata taaagtcatg
     1081 gggctcgcgc cctatggcga accaaaatat gtgcaacaaa tcttcgacca tctggtggat
     1141 ttgaaagacg atggctcatt ccggctgaac cagtcgtatt tcaactactg caccggcctg
     1201 accatgacca gcccgaagtt ccatgaccta ttcggtggcc cgcccaggaa agccgatgag
     1261 ctgctgaccc agcgccacat ggacctggcc gcatcagtgc aggccgccac ggagcagatc
     1321 gttctgcggc tcgcccgtgc catccggcaa gagaccggcc tcagcaacct gtgcctggcc
     1381 ggcggcgtgg cgctcaactg cgtagcaaat gggaaggttt tacgcgatgg ccagttcgat
     1441 gggctctgga tacagccggc ggctggcgat gccggtggcg ccttgggcgc ggcgctggcg
     1501 atacaccaca tccaccttgg ccagccccgc actgccgacg ggcgggacaa aatgcgcggc
     1561 tcctatctcg gccccgcctt cgagcaggcc gacatcgaac gccgtttgac ggctgccggc
     1621 gccaaattct cggtactcaa cgatagcgag cttccccaga ccgtagcaaa agcgctggcg
     1681 gatggccagg caataggctg gttccagggg cgcatggagt tcggccctcg cgccttgggc
     1741 ggtcgctctg tcctgggtga cgcccgctca cctgcaatgc aatcggtgct gaatctgaaa
     1801 gtaaagtacc gcgaatcgtt ccgcccgttc gcgccttccg tgttgcgcga cgacgtggcc
     1861 gactggttcg aactggatgg tgacagcccc tacatgctgc tggtggcgga tgtggtgaag
     1921 aaccgccgcc gcgtcatgac cgaggcggaa caagccctgt ttggcatcga caaactcaac
     1981 gtgccgcgtt cggaaatccc cgccgtcacc catgtggatt actcggcgcg catccagacc
     2041 gtgcatgcgg aaaccaatcc gcgctaccac gcgctgattg cggcgttcaa gcagcaaacc
     2101 ggttgcccag tgatcgtcaa caccagcttc aacgtgcgcg gcgaacccat cgtctgcacg
     2161 ccggaagacg ccttccgctg cttcatgggc accgagatcg aggtgctcgc agtaggcaac
     2221 tgtttcctga gaaaggaaga ccaggatccg gcgctcaagc ttaattatga aaccgcgttt
     2281 gaactggatt agcaccattt ccaaaaattt cagcacctgg cgaggcgggg ccatgtactt
     2341 cgccgccctg ctgatctccc tgcatgcgct cctgttctat ggcctgcctt tggcagggat
     2401 cctgtttgca tggttgcttc tggccgtctg gtttatgttc gccggggggg ctcaagcact
     2461 cttgaccgca gttccagtgg ctatattcac gctactgctc aatattagca ttcagctctc
     2521 tggcctggag cgcagcatgt actaccgtcc tcacgaattg ctcaaatcca gcagtccgga
     2581 ctttggtgac gtataccagc cgaacacccg gttcagtatg cacatgcctt tcggtgacat
     2641 tgaagccatg gaaaaggtcg gcattcagga accgcgagag atcgaatatc aaacagacgc
     2701 tctgggattc cgcaacccga ccaattacca tggccagaaa tttgtactgg ttggcgactc
     2761 ctttgtcgcc gggcttgctg acacacaatc ctgtattact acggaatggc tacgccggga
     2821 acaccacatt gacacttaca acctgggcta tcccggggac atggacgatt atgtcaaccg
     2881 tgtgcgcgcc ttccgctcgc gttatggaaa aaactttcac gtactgctct ttttttttga
     2941 aggaaacgac tttatcccct attcagcgaa gccacctcgc tcgtccagct tattgcagcg
     3001 ctattattcc tggttcaaga attctgcaat atggcgctac acgcgtgcct tatatttacg
     3061 tgggacgcaa cggcacgcag gaaagaatga gaatgcttcg ctcgtgaggg atgtaggtca
     3121 ccacccaata gccttctact ctcaagattt ggtggccgtc gccaatcggc aagcgctact
     3181 ggatagccag cttcgcttta cccaagcatt acaggcactg caacccaacc ttgcccaaat
     3241 cttttttgtg ccatcgaaat atcgggttta cgctcgttgg ctggatccca agcctttgcc
     3301 caacgagcaa tggctttacc ttgaacgcac tgcaaaacag actggcatcc cggtccataa
     3361 cctgacacct gccctcgaaa aagaagccga gcgtctgtta cctcaaggcc aatacgtcta
     3421 ctggcgcgac gacacccact ggaactgcaa cggcatgcgc acggccgcaa ccgaagtagc
     3481 gcacgtcctg agccaagact gagatgccca gccccagtca catcgatttt tcccgcatcc
     3541 tgatattggg accgtcctgg attggcgata tggtgctgtc gcaaagcctg ttcagggcca
     3601 tcaaggcgcg caacccggaa gcccgcatcg acgttgccgc cccagcctgg acgctgccgt
     3661 tgctggaacg catgccggaa gtggatgcta cgatctccct gcccttcagg cacggccaac
     3721 tggcgctggg agaacgcttc cgtttcggca agcaattgcg tgggcgcggc tatatccagg
     3781 ccatcctgct caccaattcc ctgaaatcag ccatattgcc gtttgccgcc gcgatcccgc
     3841 gccgcaccgg ttttctcggc gaattccgtt atggcctgct caatgacatc cgcccgctgg
     3901 acaaacaggc gctgccacgc acggtggatc gctttgctgc tctggggctg gagccaggtg
     3961 aacagcaacc actctcgtta cctttgcccc gcctggcggc caacccggag caggcattgc
     4021 tgacattgcg gcgactgggc catgccgcac ccgaagttcc ggtgctggga ctgtgtcccg
     4081 gcgcggaata cggtccggcc aagcgctggc cggccaccta ctacgccgag gttgccaata
     4141 cggcactggt gcggggatgg caagtttggc tgtttggttc ggaaaaagac gtgcaggtga
     4201 ccggtgaaat caacacgctg accggtagac gctgcctgga tttgggcggc agaaccacgc
     4261 tgggcgaagc cattgaccta atggcgctga ctacggaagt agtcagcaac gactctggtc
     4321 tgatgcatgt tgccgccgcg ctcggaaaac cgctggtcgc ggtttatggc tcctccgacc
     4381 cacaccacac cccaccgatg agcgaaaagg cctccattgc ctacttggga ctgccctgca
     4441 gcccatgctt caagcgtgaa tgtccgcttg ggcatttgaa ttgcctgaaa caactagcgc
     4501 cgcacgagat aatagaaaag ctgaagctgg acgtccgagt ataggagcgg gccctcgccg
     4561 cgatcaaacg agtaatcgcg ccgatggcgg cgctcctacg cataggggag tgataattag
     4621 gcttagacga tccccatgtc cttcaggcct gcagacagat ccttgtcttt tccggccttg
     4681 ccttccagct tgatccggag gcgaatgtcg ttgacagaat cggcgttgcg tagcgcatct
     4741 tcgtagctga tatggtcgtt ttcatgcagg tcgaacaacg actggtcgaa agtttgcatg
     4801 ccgagttcac gcgacctggc catgacttcc ttgatctcat gcacatcgcc cttgaaaatc
     4861 agatccgaga tcagcggcga attcagcatg atttccaccg cgggaatccg gcccttgccc
     4921 tcgacctttg gaatgagccg ttgtgaaatg aacgccttgg cattcagcga caaatccatc
     4981 agcagttgcg ggcgccgctc ttccgggaaa aagttgatga tacgatccag cgcctgattg
     5041 gtactgttgg cgtgcagggt agcaaggcac aaatggccgg tttcggcgaa agcgatggcg
     5101 taatccatgg tttcccggtc gcggatttca ccgatcagga tgacgtccgg agcctgacgc
     5161 agggtgttct tcagcgccac ataccaattg tcggtatcca ccccgacctc gcgctgggtg
     5221 atgatgcagt tcttgtgctc atgcacgaac tcgatcgggt cctcgatggt aatgatgtga
     5281 ccataactgt tctcgttgcg atggccgacc atggccgcaa gcgaagtggt cttgcccgac
     5341 ccggtgccgc cgacgaagat caccagaccg cgcttggtca tggtgatgtc cttgagcacc
     5401 tccggcagcc ctagctcctc gaacttcggg atcttgttgg taatggtccg gaacaccaga
     5461 cctggcgagc ctcgctgtac gaaagcattg acacgaaagc gcgacatgcc gggcaggctg
     5521 atggcaaagt tgcattcgtt ggtggaatcg aattccaacg cctgcttgtc gttcatgatg
     5581 ctgcggcaaa cctccttgca ttgctgcatg gtcagcgtct ggttgcttaa tggcgtgatt
     5641 tttccatcaa ccttcagtgc gggcggaaag cccacggtga tgaacaggtc ggacgcattt
     5701 ttcaccagca tcaggcgcaa caagtcgaat atgaattttt ctgtctggcc ttgctccatt
     5761 acctcatcct cctgaatgtt tccggcactt gcctagctgc cgacaaaggt gtccttgttt
     5821 tgtgccttgc cacgcgcctc ggccagagaa atcatattgc gccgcaccaa ttcctgcagg
     5881 ttctgatcca gcgtctgcat gccgacattc tggccggtct ggatggaaga atacatctgc
     5941 gcgatcttgt tttcacggat caggttgcgg atcgccggcg tgccgatcat gatttcatgc
     6001 gccgccaccc ggccggagcc atccttggtc ttgagcagcg tctgcgaaat caccgcacgc
     6061 agcgattcgg agagcatgga acgcaccatt tccttctcgg ccgccggaaa cacgtcgaca
     6121 atacggtcca ccgttttggc cgccgatgag gtatgcaagg tgccgaacac caggtggccg
     6181 gtttcggcag cggtcagtgc cagccgtatc gtttcaaggt cgcgcatttc acccaccaga
     6241 atgacgtccg ggtcctcgcg caatgccgag cgcagggcat tggcaaaaga aagcgtatgc
     6301 ggccccactt cacgctggtt gatcagacag cgtttggact gatgcacaaa ttcgatcggg
     6361 tcttccaccg tcagaatatg gccgtaatcg ttgtcgttga tatagtcgac catcgccgcg
     6421 agggtggtgg atttgcccga tccggtcggc ccggtgacca agacgatgcc gcgcggagtg
     6481 gcggcaattt ccttgaaaat ttccgggcaa ttgagttctt ccagggtcag tattttggac
     6541 ggaatagtac ggaatactgc ccctgagccg cggttatggt tgaaggcatt gacgcggaaa
     6601 cgtgccaagt tggggatttc gaaggaaaag tcgcattcca gattttcttc gtaaatcttg
     6661 cgctggccat cgctcatgat gtcgtacacc atgtcatgca cttcggtgtg atccatggat
     6721 ggcacattga ttttgcgcac atcgccatgc acgcgaatca tgggcggcat ccctgccgac
     6781 aagtgcaagt ctgaagcctt gttcttcaca gaaaatgcca gcaaatcgga aatatccatc
     6841 gacgccccct gttataattt tttcaagaca accaatgcat ggattatgac ctcaatttcc
     6901 gaacgcttgc aagcaatcca gacgtatatc cggcgggcgg agcagcaggc cggacgggag
     6961 gaggggagcg tgcagttgct cgccgtcagc aaagcgcagc cagtttctgc catatgggag
     7021 gcattccagt ccgggcaacg tctgttcggc gaaaactatc tgcaggaggc gctggagaaa
     7081 atggccgagc tcgccgacct gccgctggag tggcatttta tcggccccgt ccagagcaac
     7141 aagacacgcc cgatcgccga gaacttcgcc tgggtacacg gcgtcgatcg cctgaaaata
     7201 gccgagcgcc tgagcgaagc gcgccccccc gacctgccgc cgctgcatat ctgcctgcag
     7261 atcaatgtca gcggagaagc cagcaaaggc ggcatcgccc cgcaggaggc cggcgcgctg
     7321 gcacaggctg tttcctcgct gccaaaactg aaactgcgcg gcctcatggc catcccggca
     7381 ccgacaacgg acgttgcaat gcagcacgag cagtttagaa tgctgcggga aaccttggaa
     7441 tcattgaacg gacagggtct ctctctggac accctgtcca tgggcatgtc gcatgacttc
     7501 ccggtggcca tcgcggaagg cgcctccatc gtgcgggtag gcacggcgat tttcggcgac
     7561 aggccggcaa aggaactacg ggaaacctca tgaaaatatc tttcatcggt ggcggcaaca
     7621 tggcacgcgc catcattatc ggcctgctaa aaaggggctg ggatgccgcc tccatcaata
     7681 tcgtcgagcc ggatgccggg aaacgcagcg cattgaccac cgagttcggc gtcaccgttt
     7741 ccgaccagct gcccagcgta gcccattcgg aggtaatagt gctcgccacc aaaccacagc
     7801 agttgcgtga cgcggccatc tttcttggca gcctgctcga ccgtcagctg gtcatctcca
     7861 tcgccgccgg catccgctgc gccgatctga tccgctggct gggcaactac ggcgccgtag
     7921 tgcgtgtcat gcccaacacc ccggcacaag tacaggccgg aatttccgcc ctctacgccg
     7981 cagaaggcgt aaatgccacg cagcagcagc aagcggaaag catcatgggc gcggtcggtg
     8041 ccaccctgtg gctggaagac gagatgcaga tggatgcggt gaccgcactt tccggcagcg
     8101 gccccgccta cgtattctat tttctcgaag ccatgcaaca ggccggcgtg gatcttggtc
     8161 tgacacctgg ccaggcgggc gccctggcgt tgcagacctt tctcggtgcg gcccggcttg
     8221 ccgagggcag cgggcaggat tttgccacgc tgcggtcaca ggtcatctcc aagggcggca
     8281 ccacggagcg cgcactgaac agcctggaga acgccggggt caaagccgcc atcatccggg
     8341 cgattgatgc cgcagccaag cgttcgcgcg aactcggcga cctgctgggg aaagactgat
     8401 gagcgccctg gcccaagccg ccgccttcct gctcaacacg ctgttcggcc tgttcacgct
     8461 ggcattgctg ctgcgccttt acttgcagct ggtcaacgcg ccgttccaca acccattttc
     8521 ccaggccgtg gtgacgctaa ccaatttcgc cgtccgcccg gcgcgccgca tcattccctc
     8581 ctggggttgg ctggacaccg catccctgct gctggcgtat gcctcgcaac tgctgttgca
     8641 actgggcata ctgtggctga acgacttccc gctgctggtc gccggggcac aagtctacat
     8701 cgccttgttt gccttggccg cgcttggcat cctcaagctc agcatctaca tctttctcta
     8761 tgccgtgctg ctgttggccg tccttagttg ggtcaacccc tacacaccct tgtcaccagt
     8821 tttggacagt ctgacgcgcc cgctgctacg ccccttgcgc aaatttatta ccaagccggg
     8881 cgccatggat ctgacgccac tgatcatatt catcatcgcc gagctgctgt tgatgctgct
     8941 ggtcgcgcca ctggagcagc agttgctgaa gctgttctga ccccactccc ccgcccgcgc
     9001 ctgcagcgag cagttggagt ttccggaaaa ttcatttaaa gtcatgcgga aacgatgaat
     9061 tccgattcct ggccgggcga taacggcagc aatgccgtgt ctatttgcga aggcgcgcga
     9121 ggacgcaaag gaacggatga cgggttttca tgtttcggca ttgcggggca aaccatcagg
     9181 ccatgagcaa ctctctcgag cagcagatca ccgatttcca ccattggcgc gaccagattg
     9241 cgggggccat cgacaattat cgcgaatggc gcaatgatac cggcaacaca gacgccatcg
     9301 aggagctgcg tctttatgat ctggctgaag cgctccggcg cgaccgcctg gtactggcct
     9361 tcgtcgccga attctcgcgc ggcaaaaccg aaaccatcaa cgccctgttt ttttccgact
     9421 acaaacagcg cctgctgccc tgcgaggccg gccgcaccac aatgtgccct acggaaatat
     9481 actgggacgc gaacgaagag ccctacatca agctattgcc gattgagacg cgcaaatctg
     9541 atgagggtct tgccgacctg aaaaaaagcc ccaaggaatg gtcaaaactg cgcctggatc
     9601 ctagttccgc cacggccacg caggaagctt tccaggtgtt gagccagcaa aagcaggttt
     9661 ctctggagga ggcccgtgaa ctgggactgt gggatgacaa cgaccccgtt ctggtgcata
     9721 cgctgcaaac cctgggcaaa gtcgaagtgc cggtctggcg ccacgcgctg atcaacttcc
     9781 cgcacccgct cctgcaaagc gggttggtca tcctcgacac tcccggcctc aatgcacttg
     9841 gcaccgagcc cgacctcacc ctcagcatca ttcccaacgc acatgcggtg atattcctgc
     9901 tggccacgga taccggcgtc accaaatccg acatgcaaat ctggaacgac taccttcgcg
     9961 acaagacgag cagaaaactg gccgtactca acaaaatcga catcctatgg gatgatctca
    10021 agaccgaggc agaaatccag gccatgattc agtcccaggt agagtcctcg gccagacatc
    10081 tgggactggc aatttccgac gtcttcgcca tctcggcgca aaaggccttg ctggcacgca
    10141 tcaacggcga cagcacgctg ctgaaacgca gcggcatcga acaagtggag caggtgctgg
    10201 cacaggaatt ggtcgctgcc aagcacgtca ttctgcgcca gaccgttgta ggcgaggccg
    10261 gcaacatgat gaaagtttcg cgcagggcaa tccagctgga actgaccggc gtacgctccc
    10321 ggctgcagga actcgatgca ttgcgcggca agaaccagga cgtggtcatg ggcctgatga
    10381 ccgaggcggc ggccgatcgc aaggtgtatg aggcgaccgt gcgcaccttc gtacagggca
    10441 accagaaagt catgcaaatg ggtgaagcgt tgatgcatca gctcagcctg aggaaactgg
    10501 acgaaatgct ggagcagagc cgccggcaaa tcggcgacag ctggaccaca catggcctca
    10561 accagggcat gaagatgctg atccggcaga ccatagcaat ggcggcggac atcacgaagc
    10621 aggggcagga aatcaaggcc atcggcgatg atctctatca gctgtttcac gtcaagcacg
    10681 gcttcgagat gcggaatccg ccgatactgg acatgtccca tttccaccag agcatgatgg
    10741 cactgcaaca aaccaccgac gaattctgca acgacccggt caatgtcatg accgaaaaac
    10801 atttcctggt gcgcaaattc ttctttagcc tggcaaatct ggtacgcacc catttcgagc
    10861 aggcgcacaa cgagtccaaa tcctggctga aagaactgct ggcgccactc gcacagcagg
    10921 tcaaccagca caagtcgcag ttggacaaac gcaccgagtc actgatgacc atccacgcca
    10981 acctgaaatc cctgcagtcg aacgtcaatg cgctgatgaa tcaggaatcc agactacaaa
    11041 atcagagcac ggcgctggat catatcctgc tcaaactgat gaaggcggca cagaacagca
    11101 gcaaccccgc cccgcagcca gcgggcgcgg ccgaaagaca ggcccccggc gtgcccgcat
    11161 gacctccgcc agcctcgcct ccccggcagg ctggcgctac atcaacacga tatcgaaggt
    11221 ttcctgcccg tactgggtct ccacctgcaa tgaaatgggc ttgccgatga agtcggagag
    11281 ctgcgccaga ctttgtgact cttcgtccag gaacatctcg atcacctgct gcgacgccag
    11341 cacgcggaat tcgcgcgcgt tgaactggcg cgcctcgcgc accagttcac gcaggatttc
    11401 atagcacacc gtctgcgcgg tcttgatttc gccgcgcgcc tggcagcagg ggcatggttc
    11461 gcacaggatg tgcgccaggc tttccctggt tctttttcgc gtcagctcca ccagccccag
    11521 gccggaaaag ccatggatgc cgacacgcac ccggtctttt tccagggcgc ggcgcaactc
    11581 ttccagcacc gcctcacgat ggccctcttc ttccatgtcg atgaaatcga cgatgatgat
    11641 gccccccaga ttgcgcagcc gcagctggcg cgcgatgctc tgcgccgctt ccaggttggt
    11701 cttgaagatg gtgtcggcca gcgtcctgcc accgacgaag ccgccggtat tgacgtccac
    11761 cgtggtcagc gcctcggtct ggtcgaagat caggtagcca ccagacttca gttcgacttt
    11821 gcgcgacagt gccttctcga tttcctgctc gatgccatag agatcgaaca gcggcctttc
    11881 gccggaataa tgctcgatgc ggctgagcac gcagccggca tagtgctcgg aaaaagtcag
    11941 cattttctgc caggtttcgc gcgagtcgac cagaatgcgg tcggtatgcc cgcctaccat
    12001 gtcgcgcagc acgcgcagcg gcagcgtcag gtcctggaat accagtgatc gcgccggcgc
    12061 gtcacagcct gatgtctgga tgtcggccca taccttgtgc aggtaatcaa tgtcggcacg
    12121 cagttcatca tcctccgcgg tttccgccat ggtgcggatg atgtagccgc cccggttatc
    12181 cggcggcagc agtttgagca gacgctcacg cagcgcttca cgctcggctt cgtcctcgat
    12241 gcgctgcgac acgccgatgt gctcctgctg cggcaaatac accaggaaac ggccggcgat
    12301 gctgatctgc gtggtcaggc gcgcgccctt gctgccgatg ggctccttga tcacctgcac
    12361 catcagcgtc tgcccttcgt gcagcagttc ctggatcggc ttctcggtat tggtgtcgta
    12421 ttccaggata tcggccacat gcaggaaagc cgcgcgtgcc aggccaattt cgacgaaagc
    12481 cgactgcatc cccggcagaa cccggcatac aacccccttg tacacgttgc ctaccagccc
    12541 cagagaaccg gcacgctcca gtagcagttc ctgcaccgcg ccctgctcca tggccgccac
    12601 ccgcgtttcc tgcggggtga cgttgatcag tatttcttcg ctcaaagcag cttgactcca
    12661 aatttcttta ataactgcgc cgtctcatac accggcagcc ccatcacccc ggaatagctg
    12721 ccttcgatac ggcggatgaa ggcccctgcc agcccctgga tgccataagc gccagccttg
    12781 tcgcgcggct cgccggaggc cacataagcg gcgatctcgt cctcacccag cagcgccact
    12841 tcgacctggg tactggacag cgccacttcg acccggccat catgcgccag tgcaatggcg
    12901 gtatgcaccg catgccagcg gtcggacatg cgccgcaaca tggctgcggc atcgacgtcg
    12961 tgttccggct tgcccagaac catgccgtcg atacacaccg tggtatccgc cgccagtacc
    13021 ggacggggcg gcaacccctg caccgccagc cgctgtacgc acaccagcgc tttctctcgc
    13081 gccagccgca gcacgtaatg ctccggcgac tcgtccggca gcaccgactc gtcgacatct
    13141 gaaggcagta cctcaaaact cacaccaagc tgttccagta actctccccg gcgcggcgag
    13201 cgcgaagcca ggtatatatg cgggggcttc agcatcgctc cagcccggat tccacccgct
    13261 cccgactagc cttccccacc agcaactcca ccagcgtcag ggcaaaatcc atggctgtgc
    13321 ccgggccgcg cgaagtgaca atgttgccgt ctaccaccac cgcatcgctg cagtaatgat
    13381 gcgaacccgg caattggtcg agcacgccgg gaaaactggt ggcatttttg ccttgcagca
    13441 ggccggccgc gtgcagcgcc atcggcgcgg cgcagatggc ggcaacgtat tttccggaag
    13501 ccgccagctg cttcatcaat gcgatcactc gggcatcgtt cttcaggtgc tcgctgccgg
    13561 gcatgccgcc gggcagcacg atcatgtcga aagcctggtg cagcacctgc cccagttcgg
    13621 catccggcag cagtaccgtg ccgcgactgg caactaccgg ccccggcaac agccccgccg
    13681 taatgacttc gatgccggcg cggcgtaaga tgtcgattac cgtaacagcc tccagctctt
    13741 cgcatccctg cgcgagcgga atcaatactg tggccacttt gatgtcctgt gtgtcagttc
    13801 gcgggttgat actaacgcat ccgacaaggg ccagcaattg agcaacccgt ctggattctc
    13861 ttggattgct ggcttgagct taccctcatc aggtaaaatc gagtttcttg cactgaaaca
    13921 acccatgatc tggaaaccca acgtcaccgt cgccgccatc atcgagtgtg atggaaaatt
    13981 cctgctggtg gaggaagaga ccgccgacgg cgtgatgttc aaccagccgg ctggccatct
    14041 ggaaaacggc gaatcgctgc tggatggggt ggcacgcgag gcttgggagg aaaccagcca
    14101 tcgcttccag ccgaccgggc tgttgggcgt gtaccactgg aaacacccga agaaagacat
    14161 cacctatctg cgcttcgcct tcatcggcaa cgtcaccgac cacgatccgt cgcacccgtt
    14221 ggatgcagga atcctgcgtg cggtgtggat gactccggac gagatccgcg ccacgcagga
    14281 ccgccatcgc agtccgcaag ttctgacctg catcgaacac tatatggccg gccgctgctt
    14341 cccgttatcg gtattgaccc atttatgaaa aaacagcgcg tggtcgtcgg catgtccggc
    14401 ggcgtggatt catcggtgac cgccctgctg ctgaaacagc aaggctacga ggtggtcggc
    14461 ctgttcatga agaactggga ggacgacgac agcgaggatt tctgcccggc caaacaggat
    14521 ttgatcgacg ccgtttcggt ggcagaccgt atcggcatcg acatcgaggc ggtgaatttc
    14581 agcaaggaat acaaggacaa ggtattcgcc aattttctgc aggaatatcg cgccggccgc
    14641 acgcccaacc ccgatatcct atgcaactcg gagatcaagt tccgcgcttt tctagaccac
    14701 gccatgagca tgggcgcgga caagatcgcc accggccact atgcccaggt gcgcgaagcg
    14761 gccagcctgt tccagttatt gaaggccgaa gacggctcca aggaccagag ctacttcctc
    14821 tatcgcctca accaggagca actgtcgaaa acgctgttcc cgctgggcaa atacctcaag
    14881 cgtgaagtgc gcgagatggc cagaaaagcc ggactcgcca ccagcgagaa gaaggactcc
    14941 acgggcatct gcttcatcgg cgaacggccg ttccgcgagt tcctgcagcg ctacatgccg
    15001 accgagcctg gcgacatgca caccccggag ggaaaactgg tgggacgcca ccacggcctg
    15061 atgtactaca ccctgggtca gcgtcaggga ctgggcatcg gcggctccag ggagggtgac
    15121 ggcgagccgt ggtatgtggc ggccaaggac atggccagga acgaactggt ggtggtgcaa
    15181 gggcatgatc acccgctact gttgagcgac cgcctgaacg ccggccagat gcactggatt
    15241 tccgggagcg ccccgcgcac gcactgggtc tacgccgcaa agacgcgcta ccgccaggcc
    15301 gatgcaccgt gcgaaatcga cgccatcgat ggcgatcgct gcgaaatccg ttttggtgat
    15361 tcgcaatggg cggtgacgcc ggggcaatcg gtagtggtct acgagagcaa cgtctgcctg
    15421 ggcggcggca tcatcgaatg aacatcccgg cgtgtgccgc ctcggtaatg gcacgcacgc
    15481 cgggatgggc aaaactgcgg tcgggcatga gcgcgtagaa ttcctcgcga acttcttcca
    15541 cccgtccgac cagccttacc ccgtactgca cctcgatctc atcagccaac accgccggag
    15601 aagggaagaa gcctccccct tcctgtccga aagctttcat caacgccccg tcgtcaaatt
    15661 ctcccaccac gcgtggggca atccgcagcc gttccagcca gcgctcgata cggccacgca
    15721 ccgtggcatc gtcgcccggc atgaacaatg gcgcatcatc caggcagcgc ggaaactcgc
    15781 cacagctctc tgccaatgct ggcgcggcga agaaagccag cgcactctcc cccagcttgc
    15841 ggcttaaccc acgcactccc atgctttccg ggatgggccg gtcggccagc accaggtcca
    15901 gcttgtgcag cgataattcc gtcagcagac ggtccagttt gccctcgcgg catattagcc
    15961 gtagcggctc cgccaagtgc ctcgccggtg ccagcaggcg gtaggccagc gcttttggca
    16021 ccacgtcggt aatgccgaca cggaatggca aggccagcac cccgcgccgt gaggccaggg
    16081 cggcttccag ttcatccccc aaggcgaata tctggtcggc atagctcaac gccagacggc
    16141 ctgcctcggt tagttccagg ccgcgcccgg ctggccggaa cagcgtcgca tccagtccct
    16201 gctccagcaa tttgatttgc gcgctgatgg tttgcggagt gaggtgctga cgctcgctgg
    16261 cgcgtgccac gcttccgctg cgcgcaactg tccagaagta atgtagatgc ttgaaattca
    16321 cacgtcacct cccagacatc agaatattcg ataaatacga attatatcca atatatattc
    16381 gactttatct gatatttaat tgcgcgtatg gtgtcggtct atgtgcgtgg caagtcaatc
    16441 gccacgcaga ccatcaggag atttttatga aacgcatcat gctttttctt gccaccaacc
    16501 tggctatcgt gttggtgctg tccgtcacca tgcgcattct gggggtggag ccctatctca
    16561 atgccaacgg cctcaatctc ggagcgctgc tgacgttcgc cgcagtcatg ggtttcggtg
    16621 gctccttgat ttcactggcc atttcaaaat ggaccgccaa gatgtctgtc ggcgctcaag
    16681 tcattacaag cccgcagaat gcacaagaac gctggctagt ggaaaccgtg gaacgtcagg
    16741 cgcaggcggc aggcatcagg acgcccgagg tggcgatcta cgaatctccc gacgtcaatg
    16801 ctttcgccac cggcatgagc aagaacagtt cgctggtcgc cgtttccacc ggactgctgc
    16861 aaaagatgac gcgccaggaa gcggaagccg tcctcgggca cgaagtctcg catgcggcca
    16921 acggcgacat ggtgacgctg gcgctgatcc aaggcgtggt caacaccttc gtcatgtttt
    16981 tgtcacgcgt ggtcggatac ctggtggaca aggtggtatt caagaccgag cgcggccatg
    17041 ggccagcttt cttcgtcacc atgatcatcg ccgaactgat actgggcatc ctggcatcga
    17101 tcatcgtcat gtggttctcg cgccagcgcg aattccgcgc agaccgtggt ggggcaaacc
    17161 ttgccggacg gcaaggcatg atcaacgccc tgaaacgcct gcaatcgctg caggagccgc
    17221 agccgctacc agacaagatg gcagcatttg gcatcaacgg cgggcgtagt ggcctgatgc
    17281 gcctgttcac gacccacccg ccgctggaag agcgcatcgc cgcgctggaa gccgccgacg
    17341 acctggctgg ccactgatgg aactgtccag cgtggtcacc ccggccttct gggccgggtt
    17401 catcctgttc gtgctggcca tgctggcgct tgatctttat gtgttgggcg gcaaaaatgc
    17461 gcacaaggtt tccgcccgcg aggcgctggg ctggacactg gtctgggtaa cgctggcact
    17521 gaccttcaac ggcctgctgt ggtggtggct ggatggcacg ctgggagggg aaatcgctaa
    17581 cgagaaggcg ctggaatttc ttaccggcta cctgattgag ctgtcgctgt cggtcgacaa
    17641 cattttcgtt tttatcatga tttttactta cttcgcagtg ccggcagaat atcagcggcg
    17701 ggtgttgatc tacggggttg tcggtgccat cgtcctgcgc gccagtatga ttctggccgg
    17761 cacgctgcta gtcgcaaaat tccattggat cctttacgtc ttcggcgcat ttctggtgat
    17821 cacaggcttc aaaatgctga tcttcgccga caaggaagcc gatctcgaga gcaacccggt
    17881 actgcgctgg atgcgcggcc acctgcgcgt cacccgtgac taccacggcg aaaagttcgc
    17941 tgtcatcaag gacggtgtgc gctggttcac cccgctgttc ctggtgctca ttctgatcga
    18001 aacctcggac gtgattttcg cggttgacag cattccggcc atcttcgccg tgaccaccga
    18061 cccgttcatc gtgtttacct ccaacatctt cgccatcatg ggactgcgcg ccctttattt
    18121 tctgcttgtg gacatggcag accgcttcca cttgctgaaa tacggcctcg ccctggtgct
    18181 ggtgtttgta gggaccaaga tgctcatcgc cgatttttat aaagtgccga tcggcatggc
    18241 acttggggcg gtcgccatcc tgttgctcag cacggtcgcc gctagcctca ttgcaacccg
    18301 gcaatccaga gtaaaatcgc ctcattaaac cctttgaagg atcgctgcat gaaagtcatc
    18361 gaaagcaagc tggaaaactt tatattcaac agccgctggc tgctggcgcc gttctatatt
    18421 gggctggtgc tggcattggg gttattgctg gtgatgttcg gtagggagtt catccatttc
    18481 gtgccactgg cactgcacgg agcggaaaag gatgtgatta tcggcattct gtcgctgatt
    18541 gacgtggtga tggtggccaa ccttctcatc atcatcgtgt ttgccggcta cgaaaacttc
    18601 gtctcgcaga ttgaaaccgg tgaccatgtt gaccggccat cctggatggg gcatgtcggc
    18661 ttctccgaac tgaagatgaa gctgatcggc tctattgtcg ccatttccgg gatcgaactg
    18721 ctcagaacct tcatgcacct gacaggaacc gccacgaccg agctatcatt tggggaatgg
    18781 aacgcgctac ttgcgtggaa ggttggcatc cacctgacat tcgttgttac gggcgtgctg
    18841 ttcgcactga gcgatcgcat cggcagcgac aagaacggtg gccactgaca aaaacgtaag
    18901 gtgtcgcagc agcgacgcct tattatttca ggcggcctgg ggaacggtat ccttgaagga
    18961 aggacggctc agcagttgct ggtaaagccg atccaggttg gggtattgct cacgccagtt
    19021 gatttccggc atgcgcaggg caacataacc cagcatcact cccacagcga tatccgccag
    19081 tgtgaagcgc tcgcccatga cccacttgcc gtcgccgagg ccctctgcca gcgtggccaa
    19141 gccgcgctgc accttgagcg cctgtcgctc gatgactgtt tcatcctgcc tctccggcgc
    19201 acgccgtcct tccatcacca ccgccaccgc cgcatcgcag atgccgtccg ccagcgcctc
    19261 ccagcgacgc actacggcgc gatggttgtt gtcctgcgga atcaggcgcg cgaccggcgt
    19321 cttgtggtcc agatagtcca cgatcacgac cgaatcgtac aggctggtgc cgtcatccag
    19381 caccagagtc ggcaccttgc ccaacgggtt gtacgcgggc accggcgagt cgggcgcagc
    19441 caaggacggt acttccacca tctcgcactc aatatgcttc tcggccagca tgacgcgcac
    19501 cttgcgcgca taggggctat tgggagtcat gaacagtttc atagtcactc acggagtggt
    19561 attaagtaat tgggcattat aattcacaaa tgcagatttc ccccgcagac attgaacgaa
    19621 gcgtacgttt tgcccttgag gaagacatcg gcggcggcga cttaacggca ctcctgatcc
    19681 cgccggggca ggaagcgacg gcgtgcgtca tcagccgcga cagcgcagta ttatgtggcc
    19741 gcgcctggtt tgacgcctgt tttcgcctgc tggacaatgg cacccggatc cattggctgg
    19801 cgacggaagg ctcgacaata agccccaacc agacattatg cgaaattcgc ggcacggcca
    19861 ggagcctgct gacggcagaa cggagcgcac tcaactttct gcaaaccctg tcagccgtag
    19921 cgaccctcac ccggcgctat gtcgacgccg ttgccggtac tggcgcggcc atcatggata
    19981 cccgaaaaac cttgcccggg ctgcggctgg cgcaaaaata tgccgttact gtcgacggcg
    20041 ggaaaaacca gcgcatcggg ctctacgatg gggtgttgat caaggaaaac cacatcgcgg
    20101 cggcgggcgg tatccaacag gcgcttgccg cagcacatca ggcgtccggc gacaccccgg
    20161 tccagatcga ggtggaaacc ctggaacaac tgcaaactgc gctggatgcc ggcgcgacac
    20221 tggtgctgct ggataacttc acacacgacc agatgcgcac cgcggttgta tgcaatgccg
    20281 gccgcgcaca actggaggcc tccggtaata tcacgctgga caatgtgcgc gccgtagccg
    20341 aaaccggggt gcatcggatt tccatcggcg cattgaccaa gaacatccag gccatcgatc
    20401 tttccatgcg cttcgaagtt ggagcgtgat aggattaatc catgaagcca gatcaaccac
    20461 ccgctgccaa gagccataag ctgacgctgg gcgaggtcct gcgcctgatg gtacacgacg
    20521 gcatggtccc caaggacatt gcggaacagc tgttcaagga tcggcaactg gacagctcca
    20581 acctgcaccc gctggctgtc attgcatacc agcaatggaa gaacccgcgg tcgccgggca
    20641 agcccttcac gcttgaattc ctcaccgaat ggctggccaa ccgtattggc gtgccctatt
    20701 tccacattga tccactcaag ctcaacttca gcgcgattgc tggcatcgtt tccaaaaact
    20761 atgccgaacg gctgcgcatc atgccggtaa aagtctccga acatgaagtc gtcatcgcca
    20821 cggccgaacc tttcctcaat gattgggtag cggatctgga acgcatcctc agtcttaaaa
    20881 tccagctggt catggccaac ccactggaaa tcaaccgtta tctgccggag atttacaacc
    20941 tggccagttc agtacagatg gccgacgatg ccaaggcggg acaggtggtc ggcgtgcaga
    21001 atctggagca gttgatcgaa ctgggcaaga acaagagcct ggatgccaac gagcatcatg
    21061 tggtcaccat cgtcgactgg ttgttcgaat atgccttttc acagcgcgcc agcgacattc
    21121 atctggaacc gcgccgcgat gtcggcaacg tgcgcttccg tatcgacggc gtgctacacc
    21181 aggtctacca gttgccgccc aacatcacca acgccattac cagccgtatc aaactgctgg
    21241 gacgcatgga catggtggaa aagcgccggc cgcaggacgg gcgcatcaaa accgtgcgcg
    21301 ccgacggcac cgagatcgaa ctgcggctgt ccaccatgcc caccgccttc ggcgaaaaac
    21361 tggtgatgcg tattttcgac ccccagatcc tggtacgcga tttcaccagc ctgggctttt
    21421 cgcaggaaca gctgacgacc tggaacaatt ggaccagcgt atccaacggc atcatcctgg
    21481 tcaccgggcc aaccggctcc ggcaaaacca ctacgctata ttccaccctc aagcagctgg
    21541 cctcgccgga agtcaacgtc agcacggtgg aagacccgat cgaaatgatc gaacccgcct
    21601 tcaaccagat gcaggtccag gccaatatcg gcctcagctt cgccgatggc atacgcaccc
    21661 tgctgcgcca ggatccagac atcatcatgg tgggcgaaat ccgcgacatc gaaactgcgg
    21721 aaatggcgat ccaggccgca ctgaccgggc atctggtgct ttctacgctg cataccaacg
    21781 acgccccctc tgccatcacc cgcctgctgg aactgggtgt gccagcctac ctgatcaact
    21841 ctaccctggt gggtgtcatg gcacagcgcc tgacacgcac cctgtgcccg cagtgcaagg
    21901 aagccgggga aatccgcgag gaagcatggc aggacctggt ccatccctgg aaagtttcca
    21961 agccggcgca agtcagccgc gccgtaggct gccttgactg ccgcatgacc ggctacctcg
    22021 gccgcaccgg cctttatgaa ctgctgccaa tcagcatgga gatgcgcagg ctcatcacta
    22081 aggatgccga tctcggcaaa atcaagaccc aggcccatcg cgagggcgtg aaatcattgc
    22141 gcttcaatgg cgcggagaaa atcgcggccg gaatgacgac gttcgaggaa gtcatcaagg
    22201 ttgctcctcc cggagactag cttcgtcgcc tcccacaaaa accctacaga aaatggcctt
    22261 gaccggaaag gcccgaaacg ttactgttca gggttcgcaa cgtttacctc ttaatcaact
    22321 gaatttatgc aattaaccgg cgcagaaatc gttatccgct gcctgcagga agaaaacgtc
    22381 aagttcgttt tcggctatcc tggcggcgcg gtgctcaaca tttacgacga gattttcaaa
    22441 caggacaaat tcaagcacgt cctggtgcgc catgagcagg ccgcagtgca tgccgccgat
    22501 gcctttgccc gctccaccgg cgaagtgggt gtggcgctgg taacctccgg ccccggagcc
    22561 accaatgccg ttaccggcat cgccaccgct tacatggatt ccatcccgat ggtggtgatt
    22621 tccggccagg taccgacacc ggccatcggt caggatgcat tccaggaatg cgacatggtg
    22681 ggcatcaccc gcccctgcgt gaagcacaac ttcctggtca aggacgtcaa ggacctggcc
    22741 acgaccatca agaaggcctt ctacattgcg gccagcggcc gtcccggtcc ggtggtggtg
    22801 gatgtaccca aggacatcac gcagcacacc accaaattcg aatatccgaa gtcggtgcac
    22861 atgcgctcct acaacccggt attgaaagga cactccggcc agatcaagaa ggcgatcaag
    22921 ttgctgctgg aagccaaacg ccccatgatc tacaccggcg gcggcgtagt gctgggcgat
    22981 gcgtcagagt tgctgaccag gctggtgcgc aagctgggct accccatgac caatacgctg
    23041 atgggcctgg gcggctatcc ggctaccgac ccacaatgcc tgggcatgct cggcatgcac
    23101 ggcacgctgg aagccaacat ggcgatgcag gaatgcgacg tgatcctggc agtcggcgcg
    23161 cgcttcgatg accgggtgat cggcaaccct gagcactttt tccagaattc gcgcaccatc
    23221 atccacatcg atgtcgatcc gtcctccatt tccaagcgcg tcaaggtgga cgtgcccatc
    23281 gtcggcgacg tgaagaacgt gctggcagaa atgctggagc tgctggatct ggacgacagg
    23341 gcgcccaatg aaaacaccct caaggcctgg tggcaaaaaa tcggggaatg gcgcggcagg
    23401 aactgcctga actacgaccg caacagcacc atcatcaagc cgcagtacgt ggtggaaaaa
    23461 ctgtacgaag tgaccaaggg ggatgccttc gtcacctccg acgtcggcca gcaccagatg
    23521 tgggcggcgc agtactacaa attcgacaag ccgcgtcgct ggatcaattc cggcggcctc
    23581 gggacgatgg gggttggtct gccctacgcc atgggcgtgc agttcgcgca ccccaacgca
    23641 caggtagcct gcatcaccgg cgagggcagt atccagatga acatccagga actgtcgacc
    23701 tgcaagcagt tccacctgcc ggtgaagatc atcctgctca acaaccgcta ccttggcatg
    23761 gtgcggcagt ggcagcagtt cttctatggc aaccgctact ccgaatccta catggacagc
    23821 ctgccggatt tcgtcaagct ggcggaatcc tacggtcatg tcggtatgcg cgtagagcag
    23881 ccgggcgacg tggaaggcgc gctgcaggaa gcattcgggg caaaactgaa ggaaaaactg
    23941 gtgttcctcg atttcgtcac cgaccaaaag gaaaacgtgt tccccatggt accggctggc
    24001 aagggcttgt ctgaactgat catggcggag gaactgtaaa tgcgccatat catttcgctg
    24061 ctgatggaaa acgaatccgg cgcgctgtcg cgcgtggccg ggctgttctc ggcgcgtgcc
    24121 tacaacatcg agtcgctcac cgtggcgccg accgaggatg ccaccctgtc acgcatgacc
    24181 atcgtcactt ccggctcgga tgaagtcatc gagcagatca tcaagcagct caacaagctg
    24241 gtggacgtgg tcaaggtgct cgacctcaac gacgggcgct acctggagcg cgaactgatg
    24301 ctggtcaagg tcaaggccac cggcgcgcat cgcgaggaaa tgaagcgcat gtgcgacatt
    24361 ttccgcggcc gcatcatcga cgttgccgac ggcagctaca ccatcgagct gaccggctcc
    24421 ggctccaagc tggacgcctt cctcgaagcc atcgacaaga ccgccattct ggaaacggta
    24481 cgcactggcg cctcaggcat cgggcgcggc gaccgcgtac tgaaggttta actgatttat
    24541 ttgaaaagga aatgaaatga aggtttatta cgacaaggac gccgatctct cccttatcaa
    24601 gaagctgaaa gtcaccatcg tcggctacgg ctcgcaaggg cacgcccacg cccagaacct
    24661 gaaggactcc ggcgccaagg ttacggttgc gctgcgcaag ggcggggcct cctggagcaa
    24721 ggcggaaaag gccggccaca aggtcgagga aatcggcaag gcggtcaagg gcgctgatgt
    24781 catcatgatc ctgctgccgg acgagaccat gccggccgtg tattacgccg acgttgaacc
    24841 caacctgaag aagggcgccg tactggcttt cgcccatggc ttcaacgtgc actacaacca
    24901 gatcgtgccg cgcgccgacc tcgacgtcat catggtcgcc cccaagggac ccggccacac
    24961 cgtgcgctcc gaatacctca agggcggcgg cgtaccgtcg ctgatcgccg tgtatcagga
    25021 caagtccggc aaagccaagg atattgccct ctcctacgct gcagctaatg gcggcaccaa
    25081 gggcggcgtg attgaaacca atttccgtga agaaaccgaa accgacctgt tcggtgaaca
    25141 ggccgtgctg tgcggcggcg ccgtggaact ggtcaaggcc ggattcgaaa cgctggtgga
    25201 ggccggctac gcgccggaaa tggcctactt cgagtgcctg catgaactga agctgatcgt
    25261 cgacctgatg tacgaaggcg gcatcgccaa catgaactat tccatctcca acaacgcgga
    25321 atatggcgaa tatgtcaccg gtcccaaggt catcaacgac gaatcgcgct gggcaatgaa
    25381 ggaagccctg gaaaacatcc agaacggcga gtacgccaag cgcttcatcc tggaaggtaa
    25441 gaccaactat ccggaaatga cggctcgtcg tcgcctcaac gccgctcacc cgatcgaaca
    25501 ggtcggcggc cagctgcgcg cgatgatgcc ctggatcgcc aagaacaagc tggtggatca
    25561 gtcgaagaac taacgcaagc tataatcgca aggggcacgg cgcacaacgc cctgcccctt
    25621 tagtttttta atgggagcaa gcccttgaac aattatcctc accccatcat cgcccgcgaa
    25681 ggctggccgt ttctggccgg ttcgctggca ctctcgctcg ctgccacctt ctggtgtgcc
    25741 atgtggtcta ttccattgtg gatcatcagc ctgttcgtgc tgcagttctt ccgcgacccg
    25801 gcacgcagcg cgccatccca gaccaacgct gtcatttcgg ccgcggatgg caaggtaatc
    25861 gtggtggaaa aagcctggga tgaatacctg caacgtgacg cactcaagat cagcgtattc
    25921 atgaacgtat tcaacgtaca ctccaaccgc agcccgatcg acggccgagt ggaaaaacgc
    25981 tggtataacc ctggcaaatt cttcaatgcc gccttggcca aagcttcagc ggaaaacgaa
    26041 cgcaacgcgc tatggctgaa aacgaacgat ggccaggaca tcacctgcgt acagatcgcc
    26101 ggactgatcg cccgccgcat tctgtgctat gtcgaaccaa gcgcatcact gtacagagga
    26161 cagcgctacg gcttcatccg ctttggttcg cgcgtggata tttatctgcc gctcacggca
    26221 aaaccaaaag tcagcctggg ggaaaaggtc agagccggcg aaaccatcct ggcggaaatt
    26281 tagccagcgt cagcggagga aacccgttca ggccgacaga agcgcttgaa cgggggtggg
    26341 tcaggacgcg cgcaatgctt ttccgccagc cttccggccg gtgtgactat cagcgccgag
    26401 gtgcgtcacc aactcgttca gcgccaagcg gtagacttca cgcttgaatt cgatcacgct
    26461 gtccaacggc acccagtagt cgctccagcg ccaggcgtcg aattccgggt gctcgctggc
    26521 gcgcaaggac acatccgaat cacgtccgac caagcgcagc agaaaccaga tttgcttctg
    26581 tcccttgtag ctaccgcgcc actcgcgctt tacccagtgg gtcggtacgt tgtagtgcaa
    26641 ccaatcccgc gtgcgcccca gaatgcggac atgctccggc agcaaaccaa cctcttcggt
    26701 caattcgcga tacatggcct gctccggcgt ttccccgtgc ttgatgcccc cctgcggaaa
    26761 ctgccaggag tgctcgcgga tgcgcttgcc ccagaacacc ttgttatggg cattacacag
    26821 aatgatgccc acattcgggc gataaccgtc ccgatcgatc atgatcaata cttccaaaaa
    26881 gttagcctag attttttcac atttcggccg taattgaaag acgaactacc cacatcgatg
    26941 ctcgctatcg gcaggattgc agcattcatt gacgctgctt cgcgccgccc cgtatactcg
    27001 ccgtcaacca gccatgcgcc cagacgttga ttccccagaa tgcatccttc atcttttacc
    27061 ctaaattgat tcctcgccta tatcttgcgg tactggcctc gctgttggtg tggcttccct
    27121 tgtcgtcatg gggcatgccg tacgcctata tcaccaacca gggcagcgac aacgtgtcgg
    27181 tcatcgacct agccacagac aaggtgattg ccaccatccc cgtgggcaag gggccagtcg
    27241 gcgtcgcggt ttccggcata gggcagcgcg cctacatctc caacgtagac agcaaatcgg
    27301 tttcggtcat cgacactatg caaaaccagg tgctggagga aatacccctg gacagcgcgc
    27361 cggtcggcct ggcgctcagc ccggatggcg ccacgctcta catcgccgac tggttcgagg
    27421 acaggattct ggccatcgac accgccaacc ccggcctgcg ccgcgtgatc gcatcaggaa
    27481 aagcgcctgc gggcatggcc gtgagcaacg acggcaagcg cctgtttgtc gccaaccgcg
    27541 acagcaacca ggtcggcgtg atcgacaccg ccagcctgca aacca
//
LOCUS       DAOMFA010000003        26385 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_86_length_26385_cov_5.130611, whole genome shotgun sequence.
ACCESSION   DAOMFA010000003 DAOMFA010000000
VERSION     DAOMFA010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 26385)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26385)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26385
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86_length_26385_cov_5.130611"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(22..441)
                     /locus_tag="PKW44_00310"
     CDS             complement(22..441)
                     /locus_tag="PKW44_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HQR50062.1"
                     /translation="MKSYRKELWFNAPSRVAFINITGEVEACLRESGVSEGLVLVNAM
                     HITASVFINDDESGLHHDYGVWLEKLAPHEPVSGYHHNRTGEDNADAHMKRQIMGREV
                     VVAISGGRLDFGPWEQIFYGEFDGRRRKRVLVKVIGE"
     gene            complement(498..573)
                     /locus_tag="PKW44_00315"
     tRNA            complement(498..573)
                     /locus_tag="PKW44_00315"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(538..540),aa:Thr,seq:cgt)
     gene            complement(618..1775)
                     /gene="clsB"
                     /locus_tag="PKW44_00320"
     CDS             complement(618..1775)
                     /gene="clsB"
                     /locus_tag="PKW44_00320"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HQR50063.1"
                     /translation="MSGLVPGNRVTLLRNGTAFFPALRQAIASAQHEIHLQTYIFEID
                     NIGRVIADALMQAAARGVSVYLLLDGFGCKDMPPEFAEELQQAGIQVLFYRPKISPWS
                     LKRTRLRRLHRKVTVIDGRVAFVGGINIIDDMNTPGQKPPRVDYAVRVEGPLLAAIRA
                     SARRLWRRVAWAHLRRVDARNIKALPPPRTADGMWAAYVVRDNIWHRHDIEDAYLAAI
                     DGAAREIVIANAYFLPGIRFRRALLAAARRGVRVVVVVQARVEYLLLDYATRALYNQL
                     LTGGIEIYEYHASFMHSKVAVIDRLWATVGSSNIDPFSLMLSREANVVVEDAGFASEL
                     RHDLETAIQSGARRVRHADWQHAPRMRRLLAWVIYGLVRFTLGVIGYPDRR"
     gene            complement(1772..2524)
                     /locus_tag="PKW44_00325"
     CDS             complement(1772..2524)
                     /locus_tag="PKW44_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HQR50064.1"
                     /translation="MQRTLRIATFNIHKGVTSFNARLVLHEQRELIRRLHADIVFLQE
                     VRGAYAKHQRRFSRWPEESQYEFLADSIWQDFAYGKNAVYPAGHHGNALLSKYPIVKW
                     ENEDISAHNAEQRGMLHCEIAIPGWDQPLHGICVHLGLFARWRRQQLFALRERIETLV
                     PPHAPLIIAGDFNDWRAKAGQALAEGLHLREVFEQMGGKPARSFPAVLPLFRLDRIYV
                     RGFHVQNAQVHGGTAFSQVSDHAALSASLTRL"
     gene            complement(2534..3634)
                     /gene="nadA"
                     /locus_tag="PKW44_00330"
     CDS             complement(2534..3634)
                     /gene="nadA"
                     /locus_tag="PKW44_00330"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013149291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HQR50065.1"
                     /translation="MTQTVTIKFEKYQAAQEDDLQERILAAKAKLGKRLVILGHHYQQ
                     EGVYRHADFTGDSLKLSRYAAESDAEYIVFLGVHFMAEVADILSRPDQIAILPDMAAG
                     CSMADMANLSKVERCRRELSEHYDFDTTFTPVTYINSAADLKAFCGEHGGIVCTSSNA
                     GKIAQWAFNQREKVLFFPDQHLGRWTGHKMGIPLEQMVVWDPDLPLGGLTRQEIDDAK
                     ILLWKGHCSVHQMFRGSDITNFRAEYPDGKVIAHPECNFEVCSQSDDVGSTEYILKTV
                     SAAPAGTHWLVGTELNLVNRLAERMKPEGKTVRFMSSIVCECSTMARIDPQHLAWTLE
                     NLVEGNMVNRVHVSPQETDLAKLALDRMLEHS"
     gene            complement(3672..4121)
                     /gene="nudB"
                     /locus_tag="PKW44_00335"
     CDS             complement(3672..4121)
                     /gene="nudB"
                     /locus_tag="PKW44_00335"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HQR50066.1"
                     /translation="MPYKTPVSVLVLIHTADLQVLLLDRADKPGFWQSVTGSIEGDET
                     LRETAIREVQEETGLDAARYNLADWQLSNVYEIYPHWRYRYAPSVTQNTEHVFGLELP
                     GALPVTLNPREHLRYEWVDWREAAEKVFSWTNVEALKALGSRYGQTL"
     gene            complement(4129..4656)
                     /locus_tag="PKW44_00340"
     CDS             complement(4129..4656)
                     /locus_tag="PKW44_00340"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HQR50067.1"
                     /translation="MPVKSRIRTIPHYPRHGIQFRDITTLLKDPVGFRIAIDELHARY
                     AGQKVDKVVGVESRGFIVGAPLAYKLGVGFVPVRKQGKLPAETIGHDYELEYGSDRVE
                     IHADAIAPGEHVLLVDDLIATGGTAEAAVALIAKLGGKIVECAFLVDLPEVGGRARLE
                     QQGLKVFALCAFGGH"
     gene            complement(4665..5222)
                     /locus_tag="PKW44_00345"
     CDS             complement(4665..5222)
                     /locus_tag="PKW44_00345"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019917211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HQR50068.1"
                     /translation="MSKLTSLPDHLPVPLDDGAADHLAGMAVPTIALLATSGEMVDLA
                     GLKSWTVLYVYPMTGHPDRPLPEGWDTIPGARGCTPQSCAFRDHFAELQALGARVFGI
                     STQSTNYQREAKERLHLPFELLSDSDLLLKSALSLPTFSVTGSELYRRLTMIVVDGRI
                     KHVFYPVFPPDRNADEVINWMHNQH"
     gene            complement(5256..7040)
                     /gene="aspS"
                     /locus_tag="PKW44_00350"
     CDS             complement(5256..7040)
                     /gene="aspS"
                     /locus_tag="PKW44_00350"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HQR50069.1"
                     /translation="MRTHYCGELNRQHIGQTVTLCGWAHRRRDHGGVIFIDLRDRTGM
                     SQIVIDPDTPQAFALAETVRNEFVLKITCKVRPRPEGTVNPNIPTGEVEMLASEIEIL
                     NPSLTPPFMLDDDNLSETVRLEHRYLDLRRPAMQKNMMLRYRVAKNLRDYLDTAGFIE
                     VETPMLTRSTPEGARDYLVPSRVHHGMFFALPQSPQLFKQLLMVAGFDKYFQITKCFR
                     DEDLRADRQPEFTQVDIETSFLSENQIMDIVEDMIRKMFKQVQDIDLPNPFPRLSFAE
                     AMSRYGSDKPDMRVTLEITELTDVMKDVDFKVFSGAANSAGGRVAALRIPGGAALTRG
                     EIDAYTEFVKIYGAKGLAYIKINDTTQLNETGLQSPIVKNIHVNALQAVIDRTGAQNG
                     DLVFFGADKAKVVNEALGALRAKVGHEKGHVDGRAWAPLWVVDFPMFEHDEEADRWVA
                     LHHPFTSPKDGHEDLLTSNPGTCLSKAYDMVLNGWEVGGGSVRIHSQAVQSKVFDALK
                     ISPEEAQEKFGFLLEALQYGAPPHGGLAFGLDRLVTLMAGAESIRDVIAFPKTQRAQC
                     LMTRAPNEVDEKQLRELHIRVRQQNTAA"
     gene            complement(7065..7691)
                     /locus_tag="PKW44_00355"
     CDS             complement(7065..7691)
                     /locus_tag="PKW44_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HQR50070.1"
                     /translation="MFKRYLITGLLVLVPLAITIWVLKTLIGMMDQSLLLLPASWRPL
                     ALFGFSIPGVGAVLTLLIVLVTGMIATNFFGKRIILFWEALLARVPVVKSIYYSVKQV
                     SDTLFSDSGQAFRKALLVEYPRKGSWTIAFQTGHPGGDVARHLPGNHISVYVPTTPNP
                     TSGFFLMMAESEVIELDMSVDDALKYIISMGVVVPSDKSPPASTYAGD"
     gene            complement(7708..7962)
                     /locus_tag="PKW44_00360"
     CDS             complement(7708..7962)
                     /locus_tag="PKW44_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011310952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HQR50071.1"
                     /translation="MPIYGYRCSSCGHAEDVMQKMSDAPLTACPQCGKETYAKQLSAA
                     GFQLKGSGWYASDFKGGAKPKEEAASAPAATPCGGSCACH"
     gene            complement(8118..8591)
                     /gene="ssb"
                     /locus_tag="PKW44_00365"
     CDS             complement(8118..8591)
                     /gene="ssb"
                     /locus_tag="PKW44_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HQR50072.1"
                     /translation="MASVNKCIFIGNLGRDPEIRYMPSGDAIANFSIACTDSFKDKSG
                     QKQERTEWVRIAMFGKLAEIAGQYLKKGSSVYIEGRLQTRKWQNKEGQDQYTTEIVAD
                     RMQMLGSRSGGNAFEVMDDGGDNNAAPAARQPAPAAAKPAGGSTGFDDFEDDIPF"
     gene            complement(8593..9945)
                     /locus_tag="PKW44_00370"
     CDS             complement(8593..9945)
                     /locus_tag="PKW44_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018507865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQR50073.1"
                     /translation="MTPLELRATVALALVFALRMFGMFSILPVLAIYAQHLPGGASPF
                     LVGIALGIDGLAQAFLQIPFGLLSDRYGRKRVIYAGLALFALGSFIAAGADNIHMIIL
                     GRLVQGTGAIAAAVIALTADLTREEHRSKAMAVIGMSIGVTFGASMVAGPALAPVIGV
                     SGIFAMTGVLALLAMAVVRFVVPDPRVSHVHADAETVPGQIAAVLKNPELQRLDFGIF
                     SLHAAMRGLFVVVPLALLDIGGLAVNEHWKVYLPVMAISFLAVIPVIIIGETRGKMKP
                     VFCGAVALLLLSMLLMAVSIHNFHGVLASLFLFFLAFNLLEATLPSLVSKIAPAGSKG
                     TAIGVYNSFQFFGLFLGGALGGFLAQHVGGASVFVFAAGLCSAWLLLALSMQTPPAVR
                     TRMYHVNTMDAAGLSAQLGRLDGVVEAVVDADEGVAYLKVRLGEWDETGARNLIEREE
                     "
     gene            10038..12851
                     /gene="uvrA"
                     /locus_tag="PKW44_00375"
     CDS             10038..12851
                     /gene="uvrA"
                     /locus_tag="PKW44_00375"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HQR50074.1"
                     /translation="MDSIRIRGARTHNLKNVSLDLPRNKMIVITGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLARMEKPDVDLIEGLSPAISIEQKSTSHNPRSTVGTVT
                     EIHDYLRLLYARAGDPECPEHGIKLEAQTVSQMVDSVLALPEDTKLMILAPVVANRKG
                     EQVDLFDDLRAQGFVRVRVDGTIYELDNPPKLAKTTKHNVDVVVDRLKIRADIKQRLA
                     ESFETALRLSEGRALAVEMDSDKEQLFSAKFACPLCSYALQELEPRLFSFNNPMGACP
                     KCDGLGQISFFDPRRVVAFPHLSLASGAIKGWDRRNQFYFQMLQSLATHYGFDLDVTF
                     ESLPEAIQQVILNGSGKESIPFKYLNERGTVFQRSHAFEGILHNLERRYHETDSVMVR
                     EELAKYLNSKPCPECNGTRLRREARHVKVGESTLHQVCELPLKQALTFFHGLHLSGQK
                     LAIADKIVKEITNRLSFLTNVGLDYLSLSRSAETLSGGEAQRIRLASQIGSGLTGVMY
                     VLDEPSIGLHQRDNDRLLETLRRLRDIGNTVIVVEHDQDAILSADHVVDIGPGAGEHG
                     GQIVAQGTPQEVKDNPESLTGAYLSGRQSIAVPGKRHLPDPRRWLRVEQATGNNLKNV
                     TLKLPVGLLTCVTGVSGSGKSTLVNDTLFRSVARHLYGSSTEAAPYEEIHGLEFFDKV
                     VDVDQSPIGRTPRSNPATYTGLFTPIRELFAGVQESRVRGYGPGRYSFNVKGGRCEAC
                     QGDGVIRVEMHFLPDIYVPCDVCKGHRYNRETLEIQYKGKNIHQVLEMTVEQAHEFFH
                     AVPVIQRKLSTLLDVGLGYITLGQSATTLSGGEAQRVKLALELSKRDTGRTLYILDEP
                     TTGLHFADIQLLLNVLHRLRDHGNTIVVIEHNLDVIKTADWIVDLGPEGGDGGGLIIA
                     EGTPEQVAQNPASHTGKYLQPML"
     gene            12916..13713
                     /locus_tag="PKW44_00380"
     CDS             12916..13713
                     /locus_tag="PKW44_00380"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HQR50075.1"
                     /translation="MDFFLLIKALILGLVEGATEFLPVSSTGHLIIVGDLLHFNDGRG
                     KVFEIVIQLGAILAVCWEYRARIGHVLCNAAEPSSRRLIGNLALAFLPAALLGLAFHD
                     QIKEHLFSPVTVAWALIVGGFAILLIEKFAPQADTPTVDDMDWRHALKVGCAQAVSLI
                     PGVSRAGATILGGVVFGLSRRAATEFSFFLAIPIMFAATGYDLLKSWQDLHAGDFGVF
                     AVGFATAFFSALLVVRGLLRYVSHHDFRVFAWYRIVFGVLVLCYFQA"
     gene            13718..14344
                     /locus_tag="PKW44_00385"
     CDS             13718..14344
                     /locus_tag="PKW44_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis protein"
                     /protein_id="HQR50076.1"
                     /translation="MCDTHSLIETLHGFAEQAKERPLTLGEAVDALDEAAYAFVAIIL
                     VLPFLQPIPLGPLTVAGGLTFAVLGWQLLRGHESPVLPKRVRAVALREHTWRLMVKVS
                     LRVIGFCRTFTRPRLVHLVNGRLGRRMSGGILLAGGLLMAIPFPIPLPGNNALPGLAI
                     LFYCIGELEDDGLMVFVAFFWLIVTVLYFTAYFLALWFLGEEALAFFR"
     gene            14395..17334
                     /locus_tag="PKW44_00390"
     CDS             14395..17334
                     /locus_tag="PKW44_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HQR50077.1"
                     /translation="MNKFLKYSLIGAGGLVGLLVVGIVVLALTFNPNDYKPLIVKLVK
                     EKKQRTLNIEGDIKLAFWPKLGADLGKVSLSEHNGDKEFASMQGAKVFLAVMPLLKKE
                     LVIDTIYIDGVKANIVRYKDGSTNFDDLLSKEEESAEIKFDIDGIRVSNAALSLTDEM
                     GGRHLAIDQANIKTGHVARNQPIDLDAQFQVKADNPALNAKVQFEGILLADAEHKQYG
                     AKGMTLAVQGDVATIKALDLKLSGNLDAKPEAMEFQLDDLKLTAKANLDGKTTVLELD
                     APSLTAQKDQVNGKEAHLNLTQSQGEDTLTAKLVIADLKGSPKAFQSSGISGEISGKQ
                     GARSIAGKFSSPFSGNLEGLVFDLPKLAGNVDVKDPALPNGAMQASFNVKAHADVKQE
                     QARAALDADIDGSKLKGNVAVAGFAKPDIKFDLTADQLDLNKILGGKKESRPEAAPAK
                     PADLSALKNVLADGKLNVGLISYDKYRIANLAMTIKADGQALSVSPLSAKFDDSQIRG
                     SVGIRHFERPLYVFDLDIDRIDADRYVPPSDPKAASTAPAKPLDLSALKALNAEGSLR
                     IGSLKYGKVQGSSIRIDLKADGQKLSADPFSAKVDDSQIRASLGITRFEKPIFSFNVD
                     VDKLDADRYVTKSDQPTAKSSGDTPIDLSALKTLNASGDARLGWLKVANIRTSNVRLS
                     LKASDGLVALAPFSADLYQGAMAGSLNVDARAIPAIAFKQDMKGIQIGPLLVDAINND
                     MLEGKGTLNVDVKTQGVSVNALKKALNGTAAVVLADGAVKGIDIAGTVRDIKNKFNFK
                     GNSLGADQKKRTDFSEMTASFKITNGVAHNDDLSMKSPLLRVTGSGDIDIGNETINYT
                     ARPTVVSSLKGQGGSDLQSLDGLTFPVKVTGSFAAPKFNLDFAAVGAEITQKNLLGKV
                     GGEKGAAVQKLIGGDTAGALQGLTGGAKKDSQTAPAGTTQPAPQEPPKATPEEKAKKK
                     LNKLLGL"
     gene            17340..17762
                     /locus_tag="PKW44_00395"
     CDS             17340..17762
                     /locus_tag="PKW44_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HQR50078.1"
                     /translation="MKPWLVHAFTAMVFAGLTTVIAKQGLAGVSAELGIAVRTVFVFF
                     FVMVFVAFAVPLAEIKAIEQPSLMWLGISAFTTAISWIYFYKALKEGDVATVALIDKG
                     SVVVAVILAWIFLKEVLTLRIVLGSALIVAGLIVISRR"
     gene            17762..18802
                     /gene="mutY"
                     /locus_tag="PKW44_00400"
     CDS             17762..18802
                     /gene="mutY"
                     /locus_tag="PKW44_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013149407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HQR50079.1"
                     /translation="MSSFSNRIIAWQRRHGRHDLPWQKTRDPYAIWVSEIMLQQTQVA
                     AVIPYYQRFMARFPDIASLAAADEDIVLQHWSGLGYYSRARNLHAAAQKLMNEFGGRF
                     PQSPEAIQRLPGIGRSTAAAIAAFSFGHCGAILDGNVKRVLTRCFGVAGWPGQPAIEK
                     RLWELAESLLPQTGIAAYTQGLMDLGASLCSRSKPACSHCPLLNECVAQRECLTTSLP
                     SPKPRKVLPERATTMLLLLDGNDILLEKRPSTGVWGGLWSLPEVAGDGDAQHVARQRF
                     GLETVARPAMPTLTHAFTHFRLHITPQPLLVTKWLQQGLDSRVVWLDIEDARGAALPT
                     PVRRLLNQLRRT"
     gene            complement(18807..19853)
                     /locus_tag="PKW44_00405"
     CDS             complement(18807..19853)
                     /locus_tag="PKW44_00405"
                     /inference="COORDINATES: protein motif:HMM:NF021508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2235 domain-containing protein"
                     /protein_id="HQR50080.1"
                     /translation="MKRIVICCDGTWNSPDETDHGIPIQTNVVKVAKAVKPAAADGVE
                     QRMYYDLGVGTRGPFLKRAFDGATGSGLSENVQQAYRYLVSAYEPGDQLFFFGFSRGA
                     FTVRSLAGLIRNCGILRRDAVEMLKLGYELYRARAPGSHPRSEEATLFRRTYAISDIV
                     PIHFIGVWDTVGALGNPLMLNGYITRQQAFHDTNLSSTVGNAYHALAIDEKRKNFQAA
                     LWNQQVHAENQTLEQTWFVGVHSNVGGGYAATGLSDIALQWMVEKARDCKLELEEIPA
                     RPDPLQPREESRTAFYKLAPEFYRPIDLLDPKAGPTNERLHPSVLARYRADSSYRPKN
                     LEDYFRRNPHMRPA"
     gene            complement(19964..20518)
                     /gene="fae"
                     /locus_tag="PKW44_00410"
     CDS             complement(19964..20518)
                     /gene="fae"
                     /locus_tag="PKW44_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012777392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016840 - carbon-nitrogen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-activating enzyme"
                     /protein_id="HQR50081.1"
                     /translation="MSNKIIFKAGEATIFSEGKDVTAAMPEILIGAVDGPVGQAFANM
                     MAQSKGHTAMFAVRDINQLVRPATMMVPKVTLKDSANIELFGGVVQAATADAVLDCVI
                     EGIIPRDQANDLCIISLVWIDPGCAALAKEGTLDKADMYKNNYDATKLAIKRALNDEP
                     SIEELIKNRHTIKHCMWEDSWDQK"
     gene            complement(20582..20962)
                     /locus_tag="PKW44_00415"
     CDS             complement(20582..20962)
                     /locus_tag="PKW44_00415"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018077150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQR50082.1"
                     /translation="MSTLRRTVFIVEQKVPEALEWDGLDAIAMHVLAVSEEGIPVGCG
                     RLLPDGKIGRMAVLQGWRGRGIGRSLLQELLTMARLQGMPIASLSSQVHAIPFYEQAG
                     FRAHDPVYLDADIPHQDMSVKLTD"
     gene            complement(21018..21203)
                     /locus_tag="PKW44_00420"
     CDS             complement(21018..21203)
                     /locus_tag="PKW44_00420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR50083.1"
                     /translation="MKTFCIAVLCALLLTACAPDPDNVPKIAGSQRDALEKAKSVDAT
                     VKQNAEEQRQQVEQQTE"
     gene            21274..21663
                     /locus_tag="PKW44_00425"
     CDS             21274..21663
                     /locus_tag="PKW44_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HQR50084.1"
                     /translation="MQKFVPVIARLLLAQLFLLATLFQIIAITSHPDGYTAYQVYLGQ
                     FGLPGIFAPLTLLIQFVGGSLLLLGYRTRAAAYTMAGYALFVAFFLKLGEPIIFMQYL
                     AIAGGMLVLAGVAPTACSLDNLKKSGK"
     gene            complement(21701..22534)
                     /gene="metF"
                     /locus_tag="PKW44_00430"
     CDS             complement(21701..22534)
                     /gene="metF"
                     /locus_tag="PKW44_00430"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018078467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HQR50085.1"
                     /translation="MTAERTYSFEFFPPKTPEGMAKLRETRRQLAQLGPKFFSVTFGA
                     GGSTRDRTLETVLEIRAEGSEAAPHLSCISSTREEIRTILAQYREHGIRHLVALRGDL
                     PSGEVAASDFRYANELVEFIRQETGDWFNIEVAAYPEFHPEARSAQADLTAFKHKVDA
                     GANAAITQYFYNADAYFRFMEQCDAMGISIPVVPGIMPIYNFAQLSRFSAICGAEIPR
                     WLRLRMQDYGDDLAAVRAFGLDVVTDLCDRLLAGGAPGLHFYTLNQAGTISTIWQRLG
                     L"
     gene            complement(22541..22888)
                     /locus_tag="PKW44_00435"
     CDS             complement(22541..22888)
                     /locus_tag="PKW44_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HQR50086.1"
                     /translation="MKWLLAWIANAAALLVLAYLYPAVQVADFWTALLVAAVLGLINL
                     LVRPILILLTLPVTLLTFGLFLLVINGAMFLLADKLVDGFHISGFWPAVLAALIYSVI
                     SWLLQSLLGLRKD"
     gene            complement(22899..24308)
                     /gene="ahcY"
                     /locus_tag="PKW44_00440"
     CDS             complement(22899..24308)
                     /gene="ahcY"
                     /locus_tag="PKW44_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020162970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HQR50087.1"
                     /translation="MNAPTNFTDFKVADLSLASWGRKEIAIAETEMPGLMAIREEYAK
                     SQPLKGARITGSLHMTIQTAVLIETLTALGAQVRWASCNIFSTQDHAAAAIAADGIPV
                     FAVKGETLEEYWDYTHRIFEWADGGYSNMILDDGGDATLLLHLGSRAEQDLSVLANPS
                     SEEETILFAAIKAKLATDKTWYSIRLAQIKGVTEETTTGVHRLYQMHERGELKFPAIN
                     VNDSVTKSKFDNLYGCRESLVDGIKRATDVMVAGKIAVVAGYGDVGKGSAQALRALSA
                     QVWVTEIDPICALQAAMEGYRVVTMEYAADKADIFVTATGNYHVITHGHMKAMKDQAI
                     VCNIGHFDNEIDVASIEGYPWDEIKPQVDHVIFPDGKRIILLAKGRLVNLGCGTGHPS
                     YVMSSSFANQTIAQIELFTRTADYPVGVYTLPKHLDEKVARLQLKKLNAQLSTLTEQQ
                     AAYIGVPMQGPYKSEHYRY"
     regulatory      complement(24325..24399)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01057"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="S-adenosyl-L-homocysteine riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosyl-L-homocysteine"
                     /db_xref="RFAM:RF01057"
     gene            complement(24414..25577)
                     /gene="metK"
                     /locus_tag="PKW44_00445"
     CDS             complement(24414..25577)
                     /gene="metK"
                     /locus_tag="PKW44_00445"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HQR50088.1"
                     /translation="MSDYLFTSESVSEGHPDKVADQISDAIVDAIIAEDRHARIAAET
                     LCNTGLVVLAGEITTHANVDYIQVARDTIKRIGYDNTEYGIDYKGCAVLVAYDKQSPD
                     IAQGVNKAYDDGLDQGAGDQGLMFGYACDETPQLMPLPIWLAHRLVERQSHLRKDGRL
                     SWLRPDAKSQVTIRYVDGSPYAIDTVVLSTQHAPDMTLEAIREAVIEDIIKPVLPKEL
                     IKGEIKYLVNPTGRFVIGGPQGDCGLTGRKIIVDTYGGSSPHGGGAFSGKDPSKVDRS
                     AAYAGRYVAKNIVAAGLATRCTVQVSYAIGVAQPTSVMVETYGTGKVPNEKLTELVLQ
                     HFDLRPKGIVNMLDLLRPIYQKTAAYGHFGRDEPEFTWEATDKAAALKAAAGI"
     gene            25800..>26385
                     /gene="dapF"
                     /locus_tag="PKW44_00450"
     CDS             25800..>26385
                     /gene="dapF"
                     /locus_tag="PKW44_00450"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020163019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HQR50089.1"
                     /translation="MNLKFTKMQGLGNDFVVIDAIRQQVALTPKLVRHLADRHFGIGC
                     DQLLVVEQSTRPDADFRYRIFNADGGEVEQCGNGARCFVRFVHEQGLIDKSEIRVETA
                     RGIILPRLEDSGQVTVDMGAPRLLPEEIPFTAGALAQTYALQVGDAAVEISALSMGNP
                     HAVQIVDDIETAPVAEQGPLIERHQRFPQRVNAGF"
BASE COUNT         5137 a         7920 c         8039 g         5289 t
ORIGIN      
        1 gcccagcacc agcatgccgc tttactcgcc aatcaccttg accagcacgc gcttgcggcg
       61 gcggccatcg aactcgccgt agaaaatctg ttcccacggg ccgaaatcga ggcggccgcc
      121 actgatggcg accaccactt cgcgccccat gatctggcgc ttcatgtggg catcggcgtt
      181 gtcctcgccg gtgcggttgt ggtgatagcc gctcaccggc tcgtgcggcg ccagcttctc
      241 cagccaaacc ccgtagtcgt ggtgcaggcc ggattcgtca tcgttgatga acacgctggc
      301 ggtgatgtgc atggcattga ccagcaccag gccttcgctc acgccgcttt cgcgcaggca
      361 ggcttccact tcgccggtga tgttgatgaa ggcaacgcgg ctgggcgcgt tgaaccagag
      421 ttccttgcgg tagcttttca tgagctggat tccaaagcaa aacgggctag tcaagctaac
      481 ccgtcgtaat cattgaatgg tgccgatgac cggaatcgaa ctggtgacct tcgcattacg
      541 aatgcgctgc tctaccaact gagctacacc ggcaacagtg tgaattataa agatttagca
      601 agggcagggc aaaccgctta ccggcggtca gggtaaccaa tgacccccag cgtaaagcgt
      661 accaggccat agatcaccca ggcgagcaag cgccgcatcc ggggcgcgtg ttgccaatcc
      721 gcgtgccgca cgcgacgggc accagactgg atggcggttt caaggtcatg acgcagctcg
      781 ctggcaaaac cggcgtcttc caccaccacg ttggcttcgc gcgacagcat caggctgaac
      841 gggtcgatgt tggaggaacc gacggttgcc cacagtcggt cgatcactgc caccttggag
      901 tgcatgaagc tggcgtggta ttcgtaaatt tcgatgccgc cggtcagcaa ctggttgtac
      961 agggcacgcg tggcgtaatc cagcagcaga tattccaccc gcgcctgcac caccaccacc
     1021 acccgcaccc cacgccttgc ggcggccagc agtgcacggc ggaagcggat gccgggcagg
     1081 aaataggcat tggcgatgac gatttccctg gccgcgccgt cgatggcggc caggtaggca
     1141 tcctcgatgt cgtggcgatg ccagatgttg tcgcgcacca cataggccgc ccacatgccg
     1201 tccgctgtgc gcggcggggg aagtgccttg atgttgcgcg cgtccacccg ccgcaggtgt
     1261 gcccatgcca cgcgccgcca caagcgccgg gcgctggccc ggatcgccgc cagcagcggg
     1321 ccttccacgc gtacggcata gtccacgcgc ggcggctttt ggccgggcgt gttcatgtcg
     1381 tcaataatgt taatgccgcc gacgaaggcg acacgtccgt cgatcaccgt aaccttgcgg
     1441 tgtagccggc gcaggcgggt acgcttcagc gaccaaggtg aaattttcgg gcggtaaaat
     1501 aacacctgga tgccggcctg ttgcagttcc tctgcaaact cgggcggcat atccttgcag
     1561 ccgaagccat ccagcagcag gtaaacactt accccgcgtg ccgccgcttg catgagtgcg
     1621 tccgcaatga cacggccaat attgtcaatt tcaaagatgt aggtttgcaa gtggatttca
     1681 tgctgcgctg acgctatcgc ctgccgcagc gcagggaaaa acgccgtgcc gttgcgcagc
     1741 agggtgacgc ggttgccagg aaccaacccg ctcacagcct ggtcagggat gccgacagcg
     1801 cggcgtggtc ggaaacctgg ctgaatgcgg tgccgccgtg aacctgcgcg ttttgtacgt
     1861 gaaagccacg cacatagatg cgatccaggc gaaacagcgg cagcacggcg ggaaagctgc
     1921 gcgctggttt gccgcccatc tgctcgaata cctcgcgcag atgcagcccc tcggccagcg
     1981 cctgtcccgc cttggcgcgc cagtcgttga agtcgccggc gatgatgagg ggcgcatggg
     2041 gcggcaccag cgtttcgatg cgttcccgca gcgcgaacaa ctgttgccgg cgccagcggg
     2101 cgaacagccc gagatgcacg caaattccat gcagcggctg atcccagccg ggaatggcga
     2161 tttcgcagtg cagcatgccg cgctgctccg cgttgtgggc ggaaatgtcc tcgttttccc
     2221 acttgacgat ggggtacttg gagagcaggg cattgccgtg gtggccggcc gggtacacgg
     2281 catttttgcc ataggcgaaa tcctgccaga tagagtcggc caggaattcg tactggcttt
     2341 cttctggcca gcgagaaaag cggcgctggt gcttggcgta cgcgccgcgc acttcctgca
     2401 ggaacacgat gtcggcgtgc aggcggcgga tcagctcgcg ctgctcgtgc agtaccaggc
     2461 gcgcattaaa gctggtgacg cccttgtgga tgttgaaggt ggcgatgcga agagtacgtt
     2521 gcacgccgga gaatcaggaa tgttcgagca tgcggtccag cgcaagcttg gccagatcgg
     2581 tttcctgcgg cgacacgtgg acgcggttga ccatgttacc ctcgaccaga ttctccagcg
     2641 tccaggccag atgctgcggg tcgatgcgcg ccatggtcga gcattcgcag acgatggacg
     2701 acatgaaccg cacggtcttg ccttccggct tcatgcgctc ggccaggcgg ttgaccaggt
     2761 tgagctcggt gccgaccagc cagtgcgtac cggctggcgc cgcgctgacg gtcttgagga
     2821 tgtattcggt ggagccgacg tcgtcggact ggctgcacac ttcgaaattg cattccgggt
     2881 gcgcgattac cttgccgtcc gggtactcgg cacggaaatt ggtaatatcg gagcctcgga
     2941 acatctgatg taccgagcag tggcctttcc acaacaggat tttggcgtcg tcgatctctt
     3001 gtctggtcag cccgcccagc ggcaggtcgg gatcccaaac caccatctgc tccagcggga
     3061 tgcccatctt gtggccggtc cagcggccca ggtgctgatc cgggaagaac agcaccttct
     3121 cgcgctggtt gaacgcccac tgtgcgatct tgccggcgtt gctggaggtg cagacaatgc
     3181 cgccgtgctc gccgcagaag gccttgaggt ctgcggcaga gttgatatag gtcaccgggg
     3241 tgaaggtggt gtcgaaatcg tagtgttcgg aaagctcgcg gcggcagcgt tcgaccttgg
     3301 acaggttggc catgtccgcc atggagcagc cggccgccat gtccggcagg atggcgatct
     3361 ggtcggggcg ggagagaatg tccgccacct cggccatgaa atgcacgccg agaaagacga
     3421 tgtattcagc atccgattcg gccgcgtagc gcgacagctt gagcgagtcg ccggtgaagt
     3481 cggcgtggcg gtacacgccc tcctgctggt aatggtggcc gaggatcacc agacgcttgc
     3541 ccagcttggc cttggcggca agaatgcgct cctgcaggtc gtcttcctgc gcggcctggt
     3601 acttttcgaa cttgatggtg acggtttgtg tcatggcttt aatatcgggg attgaggtaa
     3661 aaatatcaac cttaaagtgt ttgtccgtat cggctgccga gcgctttcag cgcctcgaca
     3721 ttggtccagg aaaacacctt ttccgccgcc tcgcgccagt ccacccactc gtagcgcaaa
     3781 tgctcgcgcg ggttgagcgt gaccggcaac gctcccggca gttccagccc gaacacgtgc
     3841 tcggtattct gcgtcacgct cggcgcgtag cggtagcgcc agtgcgggta gatttcgtag
     3901 acgttggaca actgccagtc ggcgagatta taccgcgctg cgtccagccc agtctcttcc
     3961 tgcacctcgc gaatcgccgt ttcacgcagc gtttcatcac cttcgatgct gcccgtcacc
     4021 gactgccaga agccgggctt gtcggcgcga tccagcagca gcacctgcaa atccgcggta
     4081 tggatcagca ccagcacgga aaccggggtt ttataaggca ttgcggaatc aatgcccgcc
     4141 gaatgcacac aaggcgaaca ccttgaggcc ctgctgctcc agccgcgcgc ggccgccgac
     4201 ttccggcagg tcgaccagaa atgcgcattc cacaatcttt ccgcccagtt tggcaatcaa
     4261 tgccacagcg gcttctgcag tgccgccggt ggcgatcagg tcgtccacca gcagcacatg
     4321 ctcgccgggc gcgatggcgt cggcatggat ttccacgcgg tcggaaccgt attccagctc
     4381 gtagtcgtgg ccgatggtct cggccggcag cttgccctgc tttcttaccg gtacgaagcc
     4441 tacgcccagc ttgtaggcca gcggcgcgcc gacgatgaag ccgcgcgact caacccccac
     4501 taccttgtcg accttctgtc cggcataacg tgcgtgcagt tcgtcaatgg caatgcgaaa
     4561 gccgactgga tctttcagca gcgtggtgat gtcgcggaac tggatgccgt gccgcggata
     4621 gtgcgggatg gtgcggatgc gggatttgac gggcatgggg atacttaatg ctgattatgc
     4681 atccagttga ttacttcatc cgcatttctg tccggcggaa acactggata gaaaacatgt
     4741 ttgatacgcc catctacaac gatcatcgtt agtcgcctgt acagttcgct tcccgtgacg
     4801 gaaaaggttg gcaaggaaag cgctgatttc agcaacaagt cggaatcgct caataactcg
     4861 aatggcaaat gcagtctttc cttggcttcg cgctggtaat ttgtcgattg ggtgctgatg
     4921 ccgaataccc gtgcacccaa tgcttgtaac tcggcaaagt ggtcgcggaa tgcacaggat
     4981 tgcggtgtgc atccgcgcgc gccgggaatt gtatcccacc cctccggtag cggccgatcg
     5041 ggatggcctg tcatcggata tacgtatagc accgtccaac tcttcagccc agcgagatcg
     5101 accatttctc cgctggtcgc gagcagtgca attgtcggaa cagccatgcc tgctaggtga
     5161 tctgccgcac catcgtccaa aggcacggga agatggtccg gcaggctggt caacttggac
     5221 aatgcctgga atcccgattt tctgccttag cggctttagg ctgcggtgtt ctgctggcgc
     5281 acgcggatat gcagttcgcg caactgcttc tcgtccacct cgttcggcgc cctggtcatc
     5341 aggcattgcg cgcgctgggt cttggggaag gcgatgacgt cgcggatgga ttcggcaccg
     5401 gccatcagcg tcaccaggcg atccaggcca aaagccaggc cgccgtgcgg tggcgcaccg
     5461 tattgcaggg cttcgagcag gaagccgaat ttttcctggg cttcttccgg ggaaatcttg
     5521 agcgcgtcga acaccttgga ctggacggcc tggctgtgga tacgcaccga gccgccgccc
     5581 acttcccagc cgttgagcac catgtcgtag gccttggaca ggcaggtgcc ggggttgctg
     5641 gtgagcaggt cttcatggcc gtccttgggg ctggtgaacg ggtggtgcag cgcgacccag
     5701 cggtcggctt cctcgtcgtg ttcgaacatg gggaaatcca ccacccacag cggtgcccaa
     5761 gcgcggccgt cgacgtgccc cttctcgtgg ccaaccttgg cgcgcagggc gcccagggct
     5821 tcgttgacca ccttggcctt gtcggcaccg aagaacacca ggtcgccgtt ctgcgcgccg
     5881 gtacgatcga tcacggcttg cagcgcgttg acgtggatgt tcttgacgat cgggctttgc
     5941 aggccggttt cgttgagctg agtggtgtcg ttgatcttga tgtaagccag acccttggcg
     6001 ccgtagatct tgacgaactc ggtgtaggcg tcgatttcac cgcgggtcag tgcagcgccc
     6061 ccggggatgc gcagggcagc cacacggccg ccggcggaat tcgccgcgcc ggagaacacc
     6121 ttgaaatcca cgtccttcat cacgtcggtg agttcggtga tttcgagcgt gacgcgcata
     6181 tccggcttgt cggagccgta gcggctcatt gcctcggcga acgacaggcg cgggaacggg
     6241 ttgggcaggt cgatgtcctg gacctgcttg aacatcttgc ggatcatgtc ctcgacgatg
     6301 tccatgatct ggttttcgct gaggaacgaa gtctcgatat ccacctgagt gaattccggc
     6361 tggcggtcgg cgcgcaggtc ttcatcgcgg aagcacttgg tgatctggaa gtacttgtca
     6421 aagccggcca ccatcagcag ttgtttgaac agctgcggcg actgcggcaa ggcgaagaac
     6481 atgccgtgat gtacgcgcga cggcaccagg tagtcgcgcg caccttccgg cgtggaacgc
     6541 gtcagcatcg gcgtttccac ttcgatgaag ccggcagtgt ccaggtaatc acgcaggttc
     6601 ttggccacgc ggtagcgcag catcatgttc ttctgcatcg ccgggcggcg caggtcgaga
     6661 tagcggtgtt ccagacgcac ggtttcggag aggttgtcgt catccagcat gaatggcggg
     6721 gtgagcgacg gattcaaaat ctcgatctcg cttgccagca tttcgacctc gccggtcgga
     6781 atgttggggt tgaccgtgcc ttcggggcgc gggcgtacct tgcaggtgat cttgagtacg
     6841 aattcgttgc gtacggtttc ggccagggcg aacgcttgcg gcgtatccgg gtcgatcacg
     6901 atctgtgaca tgccggtgcg gtcacgcagg tcgatgaaga tcacgccgcc gtggtcgcga
     6961 cggcggtggg cccagccgca gagggtgacg gtctggccga tatgttggcg attcagctcg
     7021 ccgcagtagt gagtacgcat aattttctgg tttatcaggt tgggttaatc gccggcgtaa
     7081 gtgctggccg gcggggattt gtcggagggc acgacgaccc ccatggagat gatgtacttg
     7141 agcgcgtcat ccacgctcat gtcgagctcg atcacttcgc tctccgccat catcaggaaa
     7201 aagcccgagg tcggattggg tgtggtcggc acgtatacgc tgatatggtt gcctggcaga
     7261 tggcgcgcca cgtctccgcc cggatgcccg gtctggaatg cgatggtcca gctgcccttg
     7321 cgcggatact ctaccagcag ggccttgcgg aacgcctggc cggaatcgga aaacagcgtg
     7381 tcgctgacct gtttgacact gtaatagatg gatttcacca ccggcacccg tgccagcagc
     7441 gcctcccaga acaggatgat gcgcttgccg aagaaattgg tggcgatcat gccggtgacc
     7501 agtacgatca gcagcgtcag cacagcgcca acgcccggga tgctgaagcc gaacaaggcc
     7561 agcggccgcc aggaagccgg cagcagcagc aggctctgat ccatcatgcc gatcagcgtc
     7621 ttcaacaccc agatggtgat cgccagcggt accagtacca gcaggccggt gatcagataa
     7681 cgtttgaaca cgatggagta gggattgtta atggcaggca caactaccgc cgcatggcgt
     7741 agccgcagga gcggatgccg cttcttcctt gggcttggcg ccacctttga agtcgctggc
     7801 gtaccagccg ctgcccttga gctggaagcc ggcggcagaa agctgcttgg cataggtttc
     7861 cttgccgcac tgcgggcagg cggtcagcgg cgcgtcactc attttctgca tcacgtcttc
     7921 cgcgtggccg caggaagagc aacggtatcc ataaatcggc atggtcaata cctcctttga
     7981 aatcaatcaa aaacgcgctg aattataccc gattcagtcc tgtttcctga aattggggca
     8041 agcggactgc tttcaagaaa acccggaaaa caaaaccggc ccgcaggccg gctgttgttg
     8101 cggctggcag gtacccgcta gaacgggata tcgtcctcga aatcatcgaa acctgtgctg
     8161 ccaccagccg gtttggcggc cgctggcgcg ggctgtcttg ccgccggtgc ggcgttgttg
     8221 tcgccgccat cgtccatcac ttcaaaagcg ttgccgccgc ttcggctgcc cagcatctgc
     8281 atgcggtctg caacgatttc cgtggtgtac tgatcctggc cctccttgtt ctgccacttg
     8341 cgggtttgca agcggccttc gatgtagacc gaagaacctt tcttcaggta ttggccggca
     8401 atctcagcca gcttgccgaa catggcgatg cgcacccatt cggtacgttc ctgtttttgc
     8461 ccggatttgt ccttgaagct gtcggtgcag gcaatagaga aattcgctat cgcatcgccg
     8521 ctcggcatgt agcggatttc aggatcgcgc ccgagattgc cgatgaaaat gcatttgttt
     8581 actgatgcca tgtcattctt ccctttcgat caagttgcgt gcgccggttt catcccactc
     8641 gcccaggcgc actttcaaat aggccacacc ttcatccgca tcgaccacgg cctctaccac
     8701 accgtccagc cggcccagtt gcgccgacaa tcctgccgca tccattgtgt tcacatgata
     8761 catccgagta cgcacagcgg ggggcgtctg cattgacaaa gccagcagca gccaggcgga
     8821 acagagcccg gcagcgaaca cgaatactga tgccccgccc acgtgctgcg ccaggaaacc
     8881 gcccagtgcg ccgccgagga acagtccgaa aaactggaag ctgttgtaga cgccgatggc
     8941 ggtgcctttg ctgccggccg gggcgatctt ggataccagc gacggcagcg tggcctccag
     9001 caggttgaag gcgaggaaaa acaggaacag cgatgccagc acgccgtgga agttgtggat
     9061 ggacaccgcc atcagcagca tggacagcag cagcaaggcc actgcaccgc agaacaccgg
     9121 tttcatctta ccgcgtgttt cgccgatgat gatgaccgga atgacagcca ggaaggaaat
     9181 cgccatcacc ggcaggtaga ctttccagtg ttcgttgacc gccaggccgc cgatatcgag
     9241 cagcgccagc ggcactacca cgaacagccc gcgcatagcc gcgtgcaggc tgaagatacc
     9301 gaaatccagg cgttgcagtt ccgggttttt gagtacggcg gcaatctgtc ccggcacggt
     9361 ttcggcgtcg gcgtggacgt ggctgacgcg cggatctggt acgacgaagc gtaccaccgc
     9421 catcgccagc agcgccagca cgccggtcat ggcgaagatg ccggagacgc cgatcaccgg
     9481 agccagtgcc ggcccggcca ccatggaggc gccgaaggtg acgccgatgc tcatgccgat
     9541 caccgccatc gccttggagc ggtgttcctc gcgcgtgagg tcggcggtga gcgcgatcac
     9601 cgctgccgcg atcgcgccgg tgccctgcac cagccggccc aggatgatca tgtggatgtt
     9661 gtctgccccg gcggcgataa agctgcccag cgcgaacaac gccagaccgg cgtagattac
     9721 ccgcttgcgg ccatagcggt cggacagcag gccgaacggg atttgcagga aggcctgcgc
     9781 taggccatcg atacccaatg cgatgccgac caggaacgga ctagccccgc ccggcaggtg
     9841 ctgggcgtag atcgccagca ccggcaggat ggaaaacatg ccgaacatgc gcaaggcaaa
     9901 caccagtgcc agggcaacgg tggcgcgtag ctcgagagga gtcattttgt cggcagcgga
     9961 gtgcatgaaa attctgattg agggtaaagc tggaaattgc gtatattaac aggttggctt
    10021 tttatacgag tatcggcatg gattccatcc gcatccgcgg tgcacgcacg cataacctga
    10081 aaaacgtttc gctcgatctg ccgcgcaaca agatgatcgt gattaccgga ctatccggct
    10141 caggcaagtc ctcgctcgcg ttcgacaccc tgtatgccga aggccagcgc cgttacgtag
    10201 aatcgttgtc ggcctatgcc cgccagttcc tggcgcgcat ggaaaaaccg gacgtggacc
    10261 tgatcgaggg actttctccg gccatttcca tcgagcagaa atccacctcg cacaacccgc
    10321 gctccacggt cggcaccgtc accgaaatcc atgattattt gcgcctgttg tacgcccgcg
    10381 ccggcgaccc ggagtgcccg gagcacggaa ttaaattaga ggcccagacg gtgtcgcaga
    10441 tggtggacag tgtgctggcg ctgccagagg acaccaagtt gatgatcctc gccccggtgg
    10501 tggccaaccg caagggcgag caggtagacc tgttcgacga cctgcgcgcg cagggcttcg
    10561 tgcgggtacg ggtggacggc accatttacg agctggacaa cccgcccaag ctggccaaga
    10621 ccaccaagca caatgtcgat gttgtcgtgg accgcctcaa aatccgcgcc gacatcaagc
    10681 agcgcctggc agaatcgttc gagactgcgc tacgcctgtc ggagggccgt gcgctggcgg
    10741 tggaaatgga cagcgacaag gaacaacttt tctccgccaa gtttgcctgc ccgttatgca
    10801 gctatgcgct gcaggagctt gagccgcgcc tgttctcctt caacaacccg atgggcgcct
    10861 gtcccaagtg cgacgggctg gggcagatca gctttttcga tcccaggcgg gtggtggcgt
    10921 ttccccacct gtcattggct tccggggcca tcaagggatg ggaccggcgc aaccagttct
    10981 atttccagat gctgcaaagc ctggccacgc attacggctt cgacctcgat gtcaccttcg
    11041 agagcctgcc ggaagcaata caacaggtga tcctcaacgg tagcggcaag gaaagcatcc
    11101 cgttcaaata tctcaacgag cgcggcacgg tgttccagcg cagccatgcc ttcgagggca
    11161 tcctgcacaa cctggaacgg cgctaccacg agaccgattc cgtcatggtg cgcgaggagc
    11221 tggccaagta cctcaactcc aagccctgcc cagagtgcaa cggcacgcga ttgagaagag
    11281 aagcacgcca cgtcaaggta ggggagagca ccctgcatca ggtgtgcgaa ctgccgctga
    11341 agcaggcgct cacattcttc catggcctcc atttaagtgg gcagaagctt gccatcgccg
    11401 acaagatcgt caaggaaatc accaatcgtc tgtccttcct gaccaatgtc gggctggatt
    11461 atctgtcgct gtcgcgttcg gccgaaacac tgtccggcgg cgaggcgcag cgcatccgac
    11521 tggcctcgca gatcggctcc ggtctcactg gcgtgatgta cgtgctggac gagccttcga
    11581 ttggcctgca ccaaagggac aacgaccgcc tgctggaaac cctgcgccgt ctgcgcgata
    11641 tcggcaatac cgtgatcgtg gtggaacacg accaagacgc cattctctct gccgaccacg
    11701 tggtggacat cggccccggt gcgggcgagc acggcggcca gatcgtcgcc caaggcacgc
    11761 cgcaggaggt caaggacaac cctgagtcgc tgactggcgc ctaccttagc ggccgccaat
    11821 ctattgccgt acccggcaag cgccacttgc ctgaccccag gcgctggctg cgggtggagc
    11881 aggcgactgg taacaacctg aagaatgtca cgctcaagct gccggtcggt ttgttgacct
    11941 gtgtcaccgg cgtttccggc tccggaaaat ccacgctggt caacgacacg ctgttccgca
    12001 gcgtcgcgcg ccacctctat ggcagtagca cagaggcggc gccatacgag gaaattcacg
    12061 gactggaatt cttcgacaag gtggtggacg tggaccagtc gcccattggc cgcaccccac
    12121 gctccaaccc ggccacttac accggtttat ttacgccgat ccgagagctg ttcgccggcg
    12181 ttcaggaatc gcgggtgcgc ggctacgggc cgggacgcta ctccttcaac gtcaagggtg
    12241 gccgctgcga agcctgccag ggcgacggcg tgatccgcgt cgagatgcac tttctgccgg
    12301 acatctacgt gccatgcgac gtgtgcaagg gccaccgcta caaccgtgaa accctggaaa
    12361 tccagtacaa gggcaagaat atccaccagg tgctggaaat gacggtggaa caggcgcacg
    12421 aattcttcca cgccgtgccg gtgattcagc gcaagctaag cactttgctg gacgtcggcc
    12481 tgggctacat cacactgggg cagtccgcca ccacgctatc aggtggcgag gcgcagcgcg
    12541 tcaagctggc gctggagttg tccaaacgcg acaccgggcg caccctgtat atccttgacg
    12601 agcccaccac cggcctgcac ttcgccgaca ttcagttgct gctcaacgtg ctgcaccgcc
    12661 tgcgcgacca tggcaacacc atcgtggtga tcgagcacaa cctggatgtg atcaagacgg
    12721 ccgactggat tgtcgacctc ggccccgagg gcggggacgg tggcggcctg atcattgccg
    12781 agggcacccc cgagcaggtg gcgcagaacc cagccagcca tactgggaaa tatttgcagc
    12841 ccatgcttta agacaggccc gacggctccg tttgagccga ctgtgtagaa taacgacatt
    12901 tattaagaca ccagcatgga tttttttctc ctcatcaaag cgctgatcct cggtctggtc
    12961 gagggcgcta ccgaatttct gccggtttcc agcaccggcc acctcatcat cgtcggcgat
    13021 ctgctgcatt tcaacgatgg gcgcggcaag gtgttcgaga tcgtcatcca gctcggcgcc
    13081 atcctggcgg tgtgctggga ataccgggca cgcatcggcc atgtgctgtg taatgccgcg
    13141 gaaccttcat cgcgccggct gatcggcaat ctggcgctgg ctttcctgcc ggcggcgctg
    13201 ctggggctgg ctttccatga ccaaatcaag gagcacctgt tttcgccggt gacggtggcg
    13261 tgggcgctga tcgtgggcgg cttcgccatc ctgctgatcg agaaatttgc tccgcaagcc
    13321 gatacgccga cggttgacga tatggactgg aggcacgcac tgaaagtcgg ctgcgcccag
    13381 gcggtttcgt tgattcccgg ggtctcgcgc gccggggcga ccatcctcgg cggcgtggtg
    13441 ttcggcctgt cgcgccgggc ggcgacggaa ttctcgttct tcctcgccat tcccattatg
    13501 ttcgccgcca ccggctatga cttgctgaaa agctggcaag acctgcacgc cggcgatttc
    13561 ggcgtgttcg cggtcggctt cgccaccgcc tttttcagtg cgttattggt ggtgcgcggc
    13621 ctgctgcgct atgtgtcgca ccacgatttc cgcgtgttcg cctggtaccg tatcgtgttc
    13681 ggcgtcctgg tgctctgcta tttccaggca taaggtcgtg tgcgacacgc attccctgat
    13741 cgaaaccctg cacggttttg ccgaacaggc caaggagcgt ccgctgacct tgggggaggc
    13801 ggtcgacgcc cttgacgagg ccgcttatgc cttcgtcgcg atcatcctcg tcctgccatt
    13861 cctccagccc atcccgctgg gaccgctgac ggtagcaggt gggctcacgt tcgccgtgct
    13921 gggttggcag ctgctgcgcg gccacgagtc gcccgtcctg ccaaagcggg tgcgtgcagt
    13981 ggccttgcgt gagcatacct ggcgcctcat ggtcaaggtc agcctcaggg tcatcggatt
    14041 ctgccgtacg tttacccggc cacggctcgt gcatctcgtc aacgggagac tggggcggcg
    14101 gatgagcggg gggattctgc tggccggcgg cttgctgatg gcgattccgt tccccatccc
    14161 gctacccgga aacaatgcct tgccagggct ggccatcctg ttctattgca ttggcgaact
    14221 ggaagacgat gggctgatgg tattcgtcgc ctttttctgg ctgattgtca cagtgcttta
    14281 tttcaccgca tatttcctgg cgctgtggtt tttgggagag gaagcgcttg cctttttcag
    14341 gtaggcggcc gatgctacac tttcactcaa gacttagcca tgtagggcgc gtccatgaac
    14401 aaattcctga aatattcctt gatcggtgct ggcgggctgg tcggcctgtt ggtcgtcggc
    14461 atcgtcgtgc tggcgctcac tttcaacccc aacgactaca agccgctgat cgtcaagctg
    14521 gtcaaggaga agaagcagcg cacgcttaac atcgagggcg acatcaagct ggcgttctgg
    14581 cccaagctgg gcgccgattt gggcaaggtg tcgctgtccg agcacaacgg cgacaaggaa
    14641 ttcgcctcca tgcagggcgc caaggtgttc cttgccgtca tgccgttgct gaaaaaagag
    14701 ctggtgatcg acaccatcta tatcgatggc gtcaaggcca acattgtgcg ctacaaggat
    14761 ggcagcacca acttcgacga cctgctgagc aaggaagaag aatccgcgga gatcaagttc
    14821 gacatcgacg gcattcgtgt tagcaacgcc gcactgtcgc ttaccgacga gatgggtggc
    14881 cgccacttgg ccatcgacca ggcaaacatc aagaccggcc acgttgccag gaaccagccc
    14941 atagacctcg acgcgcaatt ccaggtcaag gcagacaatc cggccctgaa tgccaaggtg
    15001 caattcgaag gcatactgct ggctgacgcc gagcacaagc agtacggcgc caagggcatg
    15061 acgctggcgg tgcagggcga tgtggccacc atcaaagcgc tcgatctcaa gctctccggt
    15121 aacctcgatg ccaagccgga agccatggaa ttccagctgg atgacctcaa gctgacggcc
    15181 aaggccaatc tggatggcaa aaccacagta ctggaactgg acgcgcccag cctgaccgcg
    15241 caaaaagacc aggtgaacgg caaggaagcg cacctcaatc tgacgcaaag ccagggagag
    15301 gacacgctta cggccaagct cgtcattgcc gacctcaagg gttctcccaa ggcgttccag
    15361 agcagcggca tcagcggtga aatttccggc aagcagggtg ctcgcagcat tgccggaaaa
    15421 ttctcctcgc cgttctccgg caacctggaa gggctggtgt tcgatctgcc caagctggct
    15481 ggcaatgtcg acgtgaaaga cccggcgctg cccaacggcg cgatgcaggc cagcttcaac
    15541 gtcaaagccc atgccgatgt caagcaggag caggcgaggg cagcgctgga tgccgacatc
    15601 gacggcagca agctcaaggg caacgtcgcc gtggccggct tcgccaagcc cgatatcaaa
    15661 ttcgacctga ccgccgatca actcgacctg aacaagatac tggggggcaa gaaggagtcc
    15721 aggccggagg ctgctcctgc caagcctgcc gatctgtcgg cgctgaaaaa cgtactggcc
    15781 gatggcaagc tgaatgtcgg gctcatcagc tacgacaaat accgcatcgc caatctggcg
    15841 atgaccatca aggccgacgg acaggcgctg agcgtcagcc cgctgtcggc caagttcgac
    15901 gacagccaga tcaggggcag cgtcggtatc cgccacttcg agagacccct gtatgtgttc
    15961 gacctcgata tcgaccgcat cgacgccgac cgctacgtgc cgcccagcga tcccaaggcc
    16021 gcatcgaccg cgccagccaa gccgctcgac ctgtctgcgc tgaaggcgct caatgcggag
    16081 ggcagcctgc gcatcggcag cttgaagtac ggtaaggtac agggctccag catccgcatc
    16141 gatctcaagg ccgatggtca gaagctcagt gctgaccctt tttccgccaa ggtggatgac
    16201 agccaaatcc gggccagtct tggcattacc cggttcgaga agccaatttt cagtttcaac
    16261 gtggatgtgg acaagctgga cgcggatcgc tacgtgacca agagcgacca gccgacggcc
    16321 aaaagcagcg gcgatacccc gatcgacctg tcggcgctga aaaccctcaa tgccagcggt
    16381 gacgcgcgcc tgggctggct caaggtcgcc aatatcagga ccagcaacgt caggctcagc
    16441 ctcaaggcta gcgacggcct ggtcgcactg gcaccgtttt ccgccgacct ctaccagggc
    16501 gccatggccg gttcgctcaa cgtggatgcg cgcgctatcc cggctatcgc cttcaagcag
    16561 gacatgaaag gcatccagat tggcccgctg ctggtggacg cgatcaacaa cgacatgctg
    16621 gagggcaagg gcacgctcaa cgtggatgtc aaaacccagg gcgtcagcgt caatgcgctg
    16681 aaaaaggcgc tcaacggcac tgccgccgtg gtgctggctg acggtgcggt caagggcatc
    16741 gacatcgccg gcacggtgcg cgatatcaag aacaaattca atttcaaggg caattcgctg
    16801 ggtgccgacc agaagaaaag aaccgacttc agcgaaatga ccgccagctt caagatcacc
    16861 aacggcgtcg cccacaacga cgatctcagc atgaaatcgc cactgttgcg cgtcaccggc
    16921 agtggcgaca tcgatatcgg caacgagacc atcaattaca ctgccaggcc caccgtggtt
    16981 tcctcgctca aagggcaggg cggctccgat ttgcagtcac tggacggcct cactttcccg
    17041 gtaaaagtca ccggcagttt tgccgccccc aagtttaatc tcgacttcgc tgccgtcggt
    17101 gcggaaatta cgcagaaaaa cctgctcggc aaggtggggg gtgaaaaggg cgcggcagtg
    17161 caaaagctga tcggcggcga cactgccggc gcgctacaag gcctgaccgg cggcgccaag
    17221 aaagattcac agacggcgcc agccggcact acccagcccg ccccgcagga gccacccaag
    17281 gcgacgccgg aagaaaaggc gaaaaagaag ctgaacaagc tgttagggtt gtagcaaccg
    17341 tgaaaccctg gctcgttcac gcatttaccg ccatggtctt cgccgggctg accacggtga
    17401 ttgccaaaca aggactggcc ggcgtttctg ccgaactggg catcgccgtg cgcaccgtgt
    17461 tcgtgttttt cttcgttatg gtgttcgtcg ccttcgcggt gccgctggcc gagatcaagg
    17521 ccatcgagca acccagcctg atgtggctgg ggatttccgc cttcactaca gccatatcct
    17581 ggatctattt ctacaaggca ctgaaggaag gcgacgtcgc caccgttgcc ttgatcgaca
    17641 agggaagcgt ggtggtggct gtgatcctgg cctggatttt tctgaaagag gtgctgacgc
    17701 tgcgtatcgt ccttggctca gcgctcatcg tggccgggct gatcgttatc tcacggcgct
    17761 gatgtcgagc ttttccaacc gcatcatcgc ctggcagcgc cgccacggtc gccatgacct
    17821 gccttggcag aagacgcgag atccctacgc catctgggtg tcggaaatca tgttgcagca
    17881 aacccaggtc gcggccgtca ttccctatta ccagcgcttc atggcgcgct ttcccgacat
    17941 cgccagcctc gccgcagcgg atgaggacat cgtgctgcaa cactggagtg gcctcggtta
    18001 ctactcccgc gcgcgcaacc tgcatgccgc cgcacaaaaa ctgatgaacg aattcggcgg
    18061 tcgttttccg caatcgcccg aagccatcca gcgcctgccc ggcataggcc gctcgaccgc
    18121 agccgccatt gcagcgttct cattcggaca ttgcggcgcc attctggacg gtaacgtcaa
    18181 gcgcgtactc acgcgctgtt tcggagtggc tggctggccc ggccagccag ctatagaaaa
    18241 gcgactgtgg gaactggcgg aaagcctgct gccgcaaacg ggtatcgcag cctacaccca
    18301 aggactgatg gacctgggtg cctctctgtg ttcgcgcagc aaacccgcct gcagccattg
    18361 cccgctgctg aacgagtgcg tcgcccagcg cgagtgcctt accacctcgc ttccttcccc
    18421 caagccgcgc aaggtgttgc cggaaagggc aacgaccatg ctgctgctgc tcgacggcaa
    18481 cgatatcctg ctggaaaaac ggccttccac cggcgtctgg ggtggactct ggagcttgcc
    18541 ggaagtggcc ggtgatgggg atgcgcagca tgtcgcccgc caacgcttcg gactggagac
    18601 cgtggccagg cctgcgatgc cgacactcac gcacgctttc acgcatttcc gcctgcatat
    18661 caccccacag ccgctgctgg tcacgaaatg gttacagcaa ggactggatt cgcgggtggt
    18721 ctggctggat atcgaggatg cgcgcggcgc agccctgccg acgccagtgc ggagactgct
    18781 caaccagttg aggagaacct agctgttcaa gcaggccgca tgtgcggatt gcggcggaag
    18841 tagtcttcca gattcttcgg ccggtagctg gagtccgcgc gatagcgcgc cagcaccgac
    18901 ggatgcagcc gttcgttggt cgggcccgcc ttggggtcaa gcagatcgat gggccgatag
    18961 aattccggcg ccagcttgta aaaggccgtg cgtgattctt cccgaggctg caacggatcg
    19021 ggtctggcgg gaatttcttc cagttccagt ttgcaatcgc gggccttctc caccatccat
    19081 tgcagggcaa tatccgacag cccggtagcc gcatatcccc cgcccacgtt ggagtgcacg
    19141 ccgacaaacc aggtctgttc cagggtctgg ttttccgcat ggacctgctg gttccacaat
    19201 gcagcctgga aattcttgcg cttttcgtcg atcgccagcg catgataggc atttcccact
    19261 gttgaactga ggttggtgtc gtgaaacgcc tgttgccggg taatgtagcc attcagcatc
    19321 aacggattgc ccagcgcacc cacggtgtcc cagactccga tgaaatggat gggaacgatg
    19381 tcggatatgg cataggtgcg gcggaatagt gtggcttctt cgcttctcgg gtgcgagcca
    19441 ggcgcgcgtg cgcggtaaag ttcatagcct aatttcagca tttccaccgc atcacgacgc
    19501 aggatgccac agttgcggat tagcccagcc agactgcgta ccgtgaacgc gccacggcta
    19561 aagccgaaaa aaaacagttg atcaccgggt tcgtaagcag acaccagata tcggtaggcc
    19621 tgctgcacgt tttcagacag gccgctaccg gtggctccgt cgaaggcacg cttcagaaac
    19681 ggccccctag tgccgactcc cagatcgtaa tacatgcgct gctcaacgcc atcagccgcc
    19741 gctggcttga cggcctttgc cactttaacc acattggtct ggatggggat gccgtggtct
    19801 gtttcgtccg gcgagttcca ggtgccatca cagcagatga cgatacgctt catgtcggcc
    19861 tccctccaca ttggtcaacg atgttttatt tttacatgga aaccagtgcg ataccaaaac
    19921 aaaaaggcga ccgaagccgc cttttgcaaa ccggaaaaat ggattacttc tgatcccagc
    19981 tgtcttccca catgcagtgc ttgatagtgt ggcggttctt gatcagttcc tcgatggagg
    20041 gttcgtcgtt gagtgcgcgc ttgatggcca gcttagtggc atcgtagttg ttcttgtaca
    20101 tgtcggcctt gtccagcgta ccttccttgg ccagcgcggc gcaacccgga tcgatccaca
    20161 ccaggctgat gatgcacagg tcattggcct ggtcgcgcgg gatgatgccc tcgatcacgc
    20221 agtccagcac ggcgtcagcg gtggcggcct gtaccacgcc gccaaacagt tcgatattgg
    20281 cggaatcctt cagcgtcact ttcggcacca tcatggtggc cgggcgcacc agctggttga
    20341 tgtcgcgcac ggcgaacatg gcggtgtggc ccttgctctg cgccatcatg ttggcgaagg
    20401 cttggccgac cgggccgtcg acggcgccga tgaggatttc cggcatggcg gcggtgacgt
    20461 ccttgccttc gctgaaaatg gtggcttcgc cagccttgaa aatgatcttg ttggacatgg
    20521 tgtgttcctc ggaaggttgc ggaaagcgcg cattttatag aaatgcgtct tttccggcaa
    20581 atcaatcagt tagcttgacg gacatatctt ggtgtggaat gtccgcatcc aaataaacag
    20641 ggtcgtgtgc tctgaatcct gcttgttcgt aaaatgggat ggcatgaacc tgggacgaga
    20701 gtgaggctat cggcattcct tgcagccgcg ccattgtcag cagttcctgc aagagcgagc
    20761 ggccaatgcc cctgccacgc cagccctgca acaccgccat gcggccgatt ttgccatcgg
    20821 gtagcaggcg cccacagccg accggaatgc cttcttcgct aactgccagc acgtgcatgg
    20881 cgattgcatc cagtccatcc cattccagcg cttccggcac cttctgctcg acgatgaaca
    20941 cggtgcggcg tagcgtggac aaggctgtct gatgctcctg ccaggaggtt cggtaaatct
    21001 ggaacgcgtc agcaggatta ctcggtttgc tgctcaactt gttgtcgctg ttcttccgcg
    21061 ttctgcttga cggtggcatc cacactcttg gccttttcca gcgcgtcacg ctggcttccg
    21121 gcaattttgg ggacattgtc cgggtctggc gcgcaggcgg tgaggagcaa ggcgcagagt
    21181 acagcgatac agaaagtctt catgagtgcc cccttgcgac gggatatgga tgcgtgcatt
    21241 atgtggcgct ttgtcacaac aggaaacatc ccgatgcaaa aattcgttcc cgttatcgca
    21301 cgtttgctgc tggcgcagct ctttctgctt gctacgctat tccagatcat tgccatcacc
    21361 agccacccgg acggctatac cgcctatcag gtttaccttg gccagtttgg actgcccggc
    21421 atcttcgcgc cgctgaccct tctgatccag ttcgtcggcg ggagtttgct gcttctcggc
    21481 tacaggacgc gcgccgctgc ctacacgatg gccggctacg ccttgttcgt ggcattcttc
    21541 ctgaaactgg gcgagcccat cattttcatg cagtacctcg cgattgcagg cggcatgctg
    21601 gtactggctg gcgttgcccc gaccgcatgc agcctggaca atctgaaaaa atccggcaaa
    21661 taagtcacaa atgatccccg ggagcccggg gatcgcctgt ttacagcccc agacgctgcc
    21721 agatggtcga gattgtcccc gcctggttga gcgtgtagaa atgcagtccc ggcgcgccgc
    21781 cggccagcag gcggtcacac agatccgtca ccacatccag tccgaaagct cgtaccgcag
    21841 ccaggtcgtc gccgtagtcc tgcattctta gccgtagcca gcgcgggatt tccgcgccac
    21901 agatggctga gaagcgcgac agctgagcga agttgtagat cggcatgatg ccaggcacca
    21961 ccgggatgct gatgcccatg gcgtcacact gttccataaa gcggaagtag gcgtcagcat
    22021 tgtagaaata ctgcgtaatc gcggcgttcg cgcccgcatc caccttgtgc ttgaaggcgg
    22081 tcagatcggc ttgtgccgag cgtgcttccg gatggaattc cgggtaggcg gccacttcga
    22141 tattgaacca gtcgccggtt tcctggcgga tgaactccac cagttcgttg gcatagcgaa
    22201 agtcgctggc cgctacctcg cccgatggca ggtcgccgcg caaggccacc aggtgtctga
    22261 tgccatgctc gcggtattgc gccaggatgg tgcgtatctc ctcgcgggta gaagaaatgc
    22321 aggacaggtg cggcgccgcc tcggagccct cggcccggat ttccagcacg gtttccaggg
    22381 tcctgtcgcg cgtcgaaccg ccggcgccga aggtcaccga gaagaacttg ggcccgagct
    22441 gcgccagctg cctgcgcgtt tcgcgcagtt tggccatgcc ctcaggggtc ttgggcggga
    22501 agaattcaaa actgtaggta cgttcagctg tcatgttcga tcaatctttg cgcagtccaa
    22561 gcaagctttg cagcagccag gagatcacgc tgtaaatgag ggcggcgagt accgccggcc
    22621 agaagccgct gatatggaag ccgtccacca gcttatccgc cagcaggaac atggcgccat
    22681 tgatgaccag caggaatagg ccgaacgtca gcagcgttac cggcaaggtc aacagtatca
    22741 ggatgggccg caccagcagg ttgatcaacc cgagcacggc agccaccagc aaggccgtcc
    22801 agaaatcggc cacctgtacc gcagggtaga gataggccag caccaataat gctgcggcgt
    22861 tggcgatcca tgcgagcagc cacttcatgc tgtttctcct agtaccggta gtgttccgat
    22921 ttgtacgggc cttgcatcgg tacgccgata taggcggcct gctgctcggt cagcgtggag
    22981 agctgtgcgt tgagcttctt cagctgcagg cgtgcgactt tctcgtcgag atgcttgggc
    23041 agcgtgtaga cgcccaccgg gtaatccgcc gtgcgggtga acaactcgat ctgggcgatg
    23101 gtctggttgg cgaaagagct ggacatcacg tagctcggat ggccggtacc gcagcccagg
    23161 ttcaccagac ggcccttggc cagcaggatg atgcgcttgc cgtcggggaa gatgacgtgg
    23221 tcgacctgcg gcttgatctc gtcccatggg tagccttcga tgctggcaac gtcgatttcg
    23281 ttgtcaaaat ggccgatgtt gcagacgatg gcctgatcct tcatcgcctt catgtggcca
    23341 tgggtgatga cgtggtagtt gccggtggcg gtgacgaaga tgtcggcctt gtcggcagcg
    23401 tattccatgg tgactacgcg gtagccttcc atcgcggcct gcagggcgca gatcgggtcg
    23461 atctcggtca cccacacctg cgcggacagt gcacgtaatg cctgtgccga gcccttgccc
    23521 acgtcgccat agccggcgac gacggcgatc ttgccggcga ccatgacgtc ggtggcgcgc
    23581 ttgatgccgt ccaccagcga ctcgcggcag ccatacaggt tgtcgaactt ggacttggtg
    23641 acggaatcat tgacgttgat cgccgggaac ttgagctcgc cgcgctcgtg catctggtac
    23701 aggcgatgca caccggtggt agtctcctcg gtcacaccct tgatctgcgc caggcggatg
    23761 gaataccagg tcttgtcagt cgccaatttg gccttgatgg cggcgaacag gatcgtttct
    23821 tcctcggaag acggattggc caggactgac aggtcttgct cggccctgga gcccagatgc
    23881 agcagcagcg tggcatcgcc gccgtcatcc aggatcatgt tggagtagcc gccatccgcc
    23941 cattcgaaaa tgcggtgggt gtaatcccag tattcttcca gggtttcgcc cttgaccgcg
    24001 aacaccggga tgccatcggc cgcgatcgcg gcggcggcat ggtcctgggt ggagaagatg
    24061 ttgcacgaag cccagcgaac ttgggccccc agtgctgtca gcgtctcgat gagaacagcg
    24121 gtctggatgg tcatgtgcag cgagccggtg atgcgcgcgc ccttgagtgg ctggctcttg
    24181 gcatattcct cgcggatcgc catcagacca ggcatttcgg tctcggcgat ggcgatttcc
    24241 ttgcggcccc aggacgccag ggacaggtcg gcaaccttga aatcggtaaa gttggtcggc
    24301 gcgttcatag tgtttactcc tcaagatgaa cgggcgccgt tgttactgta tcgcttcgcc
    24361 aagcctggcc ggtaaccggt cgcagcgccc ctcggagaag ctggttttaa gaatcagatg
    24421 ccggcagccg ccttcagtgc agcagccttg tcggtcgctt cccaagtgaa ttccggctcg
    24481 tcgcggccga agtggccgta ggcggcggtc ttctggtaaa tcggacgcag caggtcgagc
    24541 atgttgacga tccccttggg gcgcaggtcg aaatgctgca gtaccagttc cgtcagtttc
    24601 tcattgggta ccttgccggt gccgtaggtt tccaccatca cgctggtcgg ctgcgccacg
    24661 ccaatggcgt agctgacctg cacggtgcag cgcgtggcca gtccggccgc cacgatgttc
    24721 ttggcgacat aacggccggc ataggccgcc gagcggtcca ccttggaagg gtcttttccg
    24781 gagaatgccc cgccgccgtg cggggaagag ccgccatagg tgtcgacgat gatcttgcgc
    24841 ccggtcaggc cgcagtcgcc ctgcgggccg ccgatgacga agcggccggt ggggttgacc
    24901 aggtacttga tctcgccttt gatgagctcc ttcggcagta ccggcttgat gatgtcctcg
    24961 atcaccgcct cgcggatggc ttccagtgtc atgtcgggcg cgtgctgggt ggacagcacc
    25021 acggtgtcga tggcgtaagg actgccatcc acgtaccgta tcgtgacctg agacttggcg
    25081 tcgggacgca gccaggacag gcggccatcc ttgcgcagat gggactggcg ctctaccagg
    25141 cgatgtgcca gccagatcgg tagcggcatc agttgcgggg tttcgtcgca ggcatagccg
    25201 aacatcagcc cctggtcgcc ggcgccttgg tccaggccgt cgtcataggc cttgttaacg
    25261 ccctgcgcaa tatccgggct ttgcttgtcg taggctacca gtacggcgca acccttgtag
    25321 tcgatgccgt attcagtgtt gtcatagccg atgcgcttga tggtatcgcg cgccacctgg
    25381 atgtagtcca cgttggcatg cgtggtgatc tcgcccgcca gcactaccag tccggtgttg
    25441 cacaaggtct cggccgcgat gcgggcatgg cggtcttccg caatgatggc gtcgacgatg
    25501 gcgtcggaaa tctggtcggc aaccttgtct ggatggcctt cggaaacgga ttccgaagta
    25561 aaaagataat cgctcatgag atgctccttg gcccttgagt ccggcgatac cggctccggg
    25621 ccacgagctg gcgacgcttt agcggtattt gaatcgcccc gcaagttgtc tgattaactc
    25681 ggcgagcggc tattatccat tgatccgatg tcgatttcaa ccctaagatt cccggcggta
    25741 gcatgccaaa tcctgttgcc atgtcttaca atgcaggcaa atcattccaa acaatgccaa
    25801 tgaatctgaa attcaccaaa atgcaagggc tgggcaacga cttcgttgtg atcgacgcca
    25861 ttcgccagca ggttgccttg actccgaaac tggtacgaca tcttgccgac cgccattttg
    25921 gtatcggttg tgaccagttg ctggtggtgg aacaatccac ccgcccggat gccgatttcc
    25981 gctaccgcat cttcaacgcc gatggcggcg aggtggagca atgtggcaac ggcgcgcgct
    26041 gtttcgtgcg cttcgtacat gagcagggct tgatcgacaa gagcgaaatt cgcgtcgaga
    26101 ctgcgcgcgg cattatcctg ccgcgcctgg aagacagcgg ccaggtgacg gtagatatgg
    26161 gtgcgccacg cttgctgccg gaggaaattc ctttcacggc gggtgccctg gcacagacct
    26221 atgccttgca ggttggggac gcagcagttg aaatcagcgc gctgtctatg ggcaacccgc
    26281 acgcggtgca gatcgtcgac gatatcgaaa ctgcgccagt cgccgaacag ggtccgctga
    26341 tcgagcgaca ccagcgtttt ccgcagcgcg tcaatgcggg gttca
//
LOCUS       DAOMFA010000004        23923 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_106_length_23923_cov_5.351726, whole genome shotgun sequence.
ACCESSION   DAOMFA010000004 DAOMFA010000000
VERSION     DAOMFA010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 23923)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23923)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23923
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106_length_23923_cov_5.351726"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(20..562)
                     /locus_tag="PKW44_00455"
     CDS             complement(20..562)
                     /locus_tag="PKW44_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HQR50090.1"
                     /translation="MKLEELTKIDPKNLGYLPLPVKAVLLLIALGLIVTAGFWVFWKP
                     AYEELDISRQKEAELRKVFLVKKEEAINLPAYRQQMVDIERTFGALLRQLPNKSQMDA
                     LLTDINQAGLEQGLEFELFKPGKEIPKDFYAEMPINIKLTGDFYQLGAFAYSISKLSR
                     IVTINNIKITPAGKDKDKGN"
     gene            complement(566..1141)
                     /locus_tag="PKW44_00460"
     CDS             complement(566..1141)
                     /locus_tag="PKW44_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HQR50091.1"
                     /translation="MVRVNLLPHRQIRRAELQRQFSLMFAATVAAGAAIVFLGLTYIN
                     DMKESQDERNKRLDNAIVVLDKEIEEIKRLRTEIDNVLARKKAVEALQTDRSQAVMLL
                     NEIAKQQPEGLYLKAVKQKDEKVVIEGVADSNARVAALMRNLSNSEWLESPQLVEIKS
                     VPSKGAKQNSFSVEVTQKVQKPVEPVKPKGK"
     gene            complement(1145..2215)
                     /locus_tag="PKW44_00465"
     CDS             complement(1145..2215)
                     /locus_tag="PKW44_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HQR50092.1"
                     /translation="MEFDFFKKKELPLIGVDISSAAIKMVELSEGSKGGYRVEGYAIA
                     PLPKDAVTDGNITNLEQTGEAVKRAWKLLGSRERRVALALPVAAVISKKVIMQADLRE
                     EDMELRVEAEANQYIPFSLDEVNIDFQVVGPASSSEDEVEVLIAASRKEKIEDRVASV
                     EAAGLKAVVMDVETYATEAAYQLAAKQLPQGGREQTVMVVDIGAFMMHVNVLHNNESV
                     YIREQNFGGNQLTLEIQRRFGLSAEEAEIAKRKGGLPESYEPELLQPFVQSLAMEVAR
                     AVHFFTSSTQFNQIDHVMLAGGCAAIPGVDQAVADRVRVNTIIANPFINMSFSNRVNQ
                     QSLLAEAPSLMIACGLALRRFD"
     gene            2378..4720
                     /locus_tag="PKW44_00470"
     CDS             2378..4720
                     /locus_tag="PKW44_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HQR50093.1"
                     /translation="MFPKKWWQLLLLSLLSAGVAVSALIALAVVLIYPDLPSLETLTD
                     YRPKIPLRVYSEDGYLISEFGEERRALVKINDVPKEMKDAILAIEDRRFYQHGGIDTV
                     GVLRAILSNLTSGDAREGASTITMQVARNFFLSSEKTLKRKAGEALLAMKIEQNLSKD
                     QILELYINQIYLGQRAYGFSAAAQVYFGKPLNKLNLAEMAMLAGLPKAPSRYNPFTNM
                     KRAQSRQQDVLRHMWRFNFIDERTYQAALKQPIHLKASRQSRDLAADYAAEIVRQQLY
                     DRYQESIYSSGLKVYTTILKANQEAANKAVLQGVLDYDKRHGYRGPEGKINLREAPQG
                     AMETFLREAMADVEEYMELEPAVVLEVNASSIKAFRKKGEVININGDGLLLVKTMLSD
                     KNPSKLKPQPGSLIRVKRQGDNWQIVQLPQVEAALIAVDPKNGAVRALVGGFDFSRNK
                     FNHTTQAWRQPGSSFKPFIYSAALEKGFTPASVIEDEPLVISAEETGSGKAWEPHNFD
                     AKFEGPMRMRTALTKSKNMVSIRILQAIGVNYAQDYITRFGFAAKDHPAYLAMALGAG
                     SVTPWQMAGAYATFANGGYRVTPYLINKIVDNNGKIMEQAKPVIAGVNAQRVIDPRNA
                     FIMTSMMQDVARIGTAAKARELGRYDLAGKTGTTNNHMDAWFAGYNPTQVAVAWIGFD
                     QPRSLGNRETGGAAALPMWISYMSRALQGVPDVAYQVPDGIASIKINLATGERATEYQ
                     GGLYEYFYLENPPPAADANSEQAAETGAKADEEPGPEQLF"
     gene            4735..5340
                     /locus_tag="PKW44_00475"
     CDS             4735..5340
                     /locus_tag="PKW44_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013149234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR50094.1"
                     /translation="MPSNIRPQHLRQMIAQLAARMIAEDGAQDYAHAKRKAARQLAVS
                     DTHCLPTNSEIEQELRLHHEIFRSAEQPRHLFRLRNEALAVMRLLERFNPHLVGAVLE
                     GTAGRYAETEIHLFADSLKDVELFLLNQGIQYRMEEKTYRFGNERRSLPRFSLEGAHG
                     VIQVTVFATDDLRVPPKSRLRNGSQERADTPTVAAMVALNL"
     gene            complement(5329..6333)
                     /gene="hemB"
                     /locus_tag="PKW44_00480"
     CDS             complement(5329..6333)
                     /gene="hemB"
                     /locus_tag="PKW44_00480"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HQR50095.1"
                     /translation="MSHAYGKYPSTRPRRMRRDDFSRRLMRENVLTANDLIYPVFVLD
                     GESRSEAVASMPGVQRQSVDVLLKTAEECVRLGIPALALFPVVEQSRKSLDAAEAYNP
                     DGLVPRTIRALKQAFPELGVITDIALDPYTSHGQDGLTDESGYVLNDETVAVLVQQAL
                     CHAQAGADVVAPSDMMDGRIGAIRQALEFNGHIHTRILAYSAKYASAFYGPFRDAVGS
                     AANLGKGDKTTYQMDPANSDEALKEVALDLEEGADMVMVKPGMPYLDIVRRVKEEFGV
                     PTFAYQVSGEYAMLKAAAQNGWLDEKACVLEALLSFKRAGADGILTYFARDVARWLKV
                     "
     gene            complement(6343..7131)
                     /gene="trpC"
                     /locus_tag="PKW44_00485"
     CDS             complement(6343..7131)
                     /gene="trpC"
                     /locus_tag="PKW44_00485"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HQR50096.1"
                     /translation="MSDILKKILATKQEEVNAANVLQPLGLVRSKAESATAPRDFIGA
                     IRAKIAAGQAAVIAEIKKASPSKGVIREDFRPAEIAVTYAAHGAACLSVLTDTQYFRG
                     SPEYLKQARGACNIPVLRKDFMIAPYQVYEARAMGADCILLIAAALDLPRMKELENIA
                     HDLGMAVLVEVHDGEELDQALQLETPLIGINNRNLRTFDVTLDTTLGLLSRIPQDRIV
                     VTESGIFTPQDVALMRSHNVHTFLVGEAFMRQPDPGVELARVFG"
     gene            7289..7477
                     /locus_tag="PKW44_00490"
     CDS             7289..7477
                     /locus_tag="PKW44_00490"
                     /inference="COORDINATES: protein motif:HMM:NF025012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HQR50097.1"
                     /translation="MSVLIKPLQQQIPWTKIKSETVGEETFMRAFLLSILLCLALTAC
                     GAKGPLYLPEKQYPQGNQ"
     gene            7474..8721
                     /gene="lysA"
                     /locus_tag="PKW44_00495"
     CDS             7474..8721
                     /gene="lysA"
                     /locus_tag="PKW44_00495"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HQR50098.1"
                     /translation="MNPFESINGELHAENTPLSQIAAQFGTPCYVYSRTALTEAFQRF
                     DAGFSGHDHLVCYAVKANPNLAILNLFARLGAGFDIVSGGELARVLAAGGDPGKVVFS
                     GVGKTAAEMRAALQAGIFCFNVESSAELERLNTVAGEMNKRAPVSLRVNPNVDAKTHP
                     YISTGLKNNKFGVAYEEAFALYQRAADLPNIEIHGVDCHIGSQLTELSPFLDALDRVL
                     ALVDQMESSGIHLRHLDIGGGIGIRYADETPPDFANYSRAILDKVGGRNFKLVMEPGR
                     ALVGNAGLLLTTVEYLKPGESKNFAIVDAAMNDLMRPALYDAWHEILPVRARQDTEQI
                     YEIVGPVCESGDFLGHDRKLSLQQGDLLAILSAGAYGMSMSSNYNTRPRAAEVMVDGR
                     QLHLVRERETLLQLFSLEHILTT"
     gene            complement(8732..9586)
                     /gene="purU"
                     /locus_tag="PKW44_00500"
     CDS             complement(8732..9586)
                     /gene="purU"
                     /locus_tag="PKW44_00500"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HQR50099.1"
                     /translation="MKNTATLLINCPDRKGLVAAIADFLYRHDANILHADQHQDAENN
                     LFLMRVEWDLNGFGIAPETFAEHFSPLAQRFQMQWQLKLSQQRPRMAIMVSHYDHCLV
                     DLLYRHQSGELACDIPLIISNHKATQRLAEFYGIPFHHIEVTKDSKTEAEARQFALFD
                     QHQIDLIVLARYMQILSPEFVRRYPQRIINIHHSFLPAFIGARPYHRAFERGVKLIGA
                     TSHYVTEVLDEGPIIEQDLMRVSHRDQIDDLIQKGRDLEKVVLSRAVRWHVESRILLY
                     ANKTVIFD"
     gene            9701..10219
                     /locus_tag="PKW44_00505"
     CDS             9701..10219
                     /locus_tag="PKW44_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HQR50100.1"
                     /translation="MKKQWPYFVLAFAIPILLVLWWWGLFTTATIEVTTLGPYRYAYL
                     EAQGAYSKLAGKQREVMFELQKQGITPEGSITLILDDPRTTPYDKLVARTGYQIATSA
                     TPASPLALETIPAREVVVAQVRAHPLFAYGKAYSALLDYTRQHGMALRMPTLERYSNS
                     VLTVEMPVERSP"
     gene            10216..11127
                     /locus_tag="PKW44_00510"
     CDS             10216..11127
                     /locus_tag="PKW44_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CobD/CbiB family protein"
                     /protein_id="HQR50101.1"
                     /translation="MSFFVLLAALVLNHYRPLSRTDLLQAVFGPYANLLERNFNDGQA
                     RHGLVAWLLAILPPVLLIGAVYFVLYRLNILLGMLFAIAVLYVTPRFGRFSTPPEQIA
                     ALLHEGRLDDARERLRQWQSLDTHDYDAGQVARVSIESTLCQAHHDFIAPVFWFVLLG
                     PAGAVLYRLAHLLRNVWGNKSGAFGDFSKRAFDWLDWLPARFTAGCFAVMGDFEDAVY
                     CWRIQAFAWPDRAVGIMLASGAGALGVRLGAAIPRDGNMEYRPELGLGDEADADYLHA
                     ASGLVWRVLALILGLLLLLTFAHWLGK"
     gene            11137..12060
                     /gene="ispH"
                     /locus_tag="PKW44_00515"
                     /gene_synonym="lytB"
     CDS             11137..12060
                     /gene="ispH"
                     /locus_tag="PKW44_00515"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HQR50102.1"
                     /translation="MKVLLANPRGFCAGVDRAIIIVEQALERFGAPIYVRHEVVHNKF
                     VVDELKRKGAVFVEELDEVPTGNTVIFSAHGVSKDVRAEAEVRGLRVFDATCPLVTKV
                     HVEVARMRAAGREIIMIGHKGHPEVEGTMGQSEGGMYLVETPEDVGRLQVADPDKLAY
                     VTQTTLSIDDAAAIVDALKARFPGIAAPKSDSICYATQNRQDAVKTLAHGCDLVIVVG
                     SPNSSNSNRLREVARNQGVESYMVDNASQLQLEWLAGKQRVGVTAGASAPEVLVQEVI
                     ARLSALGASDVHELEGIIEHVVFPLPKNLAA"
     gene            12057..12677
                     /locus_tag="PKW44_00520"
     CDS             12057..12677
                     /locus_tag="PKW44_00520"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0106141 - flavin prenyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HQR50103.1"
                     /translation="MSKTFTLALTGASGMPYAMRLLECLLGAGHTVYLVYSQAAQFVA
                     AQEMAISLPAKPSEAHRLLVERLHCRPQQLLVFAREDWNAPIASGSGVADAMVVCPCT
                     MGCLAAIANGLSDNLIERAADVMLKEGRKLILVPRETPFSAIHLENMLKLARLGTVIL
                     PPNPGFYHQPVHLDDLVDFVVARILDQLGVEHRLVKRWGSDNAQCS"
     gene            complement(12692..13150)
                     /locus_tag="PKW44_00525"
     CDS             complement(12692..13150)
                     /locus_tag="PKW44_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HQR50104.1"
                     /translation="MFEVLVYLFENYFEANIRPDQDTLARELFAAGFDENDISSAFDW
                     FTTLEEMTSHMAASCHEGGNGLRIYADVESRKINLECQGFLVFLEQAGVIDAAQRELA
                     IDRAMALPDGKVTLEQLKWIMLMTMWNQGKARDYLFVEDAVFSDMRPTMH"
     gene            complement(13175..14266)
                     /gene="dprA"
                     /locus_tag="PKW44_00530"
     CDS             complement(13175..14266)
                     /gene="dprA"
                     /locus_tag="PKW44_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HQR50105.1"
                     /translation="MAEALPGTTPEDAAQWVGLSLIAGLGRQGLRALLSAFGTPERIY
                     AIPYAELHKVVGGPVAQQIAAGLDHASIRPVLEWLEQPGNHLITLADTAYPKALLEIS
                     DPPPLMYAKGRIELLSQPSLAVVGSRNATPQGEKTAEAFSQVLSDNGYCIVSGLALGI
                     DGAAHRGALQGRASTISVVGTGLDIVYPARHRQLAHQISERGLLISEFPLGTPSMAQN
                     FPRRNRIISGLSQGCLVVEAHLQSGSLITARLAAEQGREVFAIPGSIHSPVAKGCHLL
                     IKQGAKLVESMQDILEELGVAVPRAEDVALAQADEPLLGHMGYDPIGMDELVARSGLT
                     SENLSAMLLILELEGKVACLPGGRYQRIS"
     gene            complement(14266..15306)
                     /locus_tag="PKW44_00535"
     CDS             complement(14266..15306)
                     /locus_tag="PKW44_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HQR50106.1"
                     /translation="MNKYIIALLLLCCLAPVARADNIQLQDGHPDRYVVVKGDTLWGI
                     AGRFLKDPWRWPQIWKMNREQIKNPHLIYPGDVVVLDVDSNGQPQLRLLRETITLEPG
                     TMVEPLEKKPIPTISPGAIAPFLTQPLIVSEEELQQAPSIVGAEGGHVALTSGYNIYV
                     DHIEEGGGLNWQIFRKGNALVDPDTKEQLGFETVYLGDARAVRYGDPATIQIVRPKEE
                     VYKGDKLFKMPDTDMNSFVPRAPESDIAGRIISAYNAIAELGPNSVVIINRGSDDGLA
                     EGEVLAIYRPGTVVPAPASKKGDKPKPLLLPDERIGLLMVFRAFKRVSYAIIMKAERP
                     VNVLDVVRTPTP"
     gene            15394..15897
                     /gene="def"
                     /locus_tag="PKW44_00540"
     CDS             15394..15897
                     /gene="def"
                     /locus_tag="PKW44_00540"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HQR50107.1"
                     /translation="MAILSILQYPDERLHTVAQPVTEVEARVRRLVADMAETMYAAPG
                     IGLAATQVNVHQQVIVIDISEEKNQLMVFINPRIVTSGSMRDYEEGCLSVPGIYEKVR
                     RPEQVTVEALDADGKPFTRQADGLLAVCIQHEMDHLKGKVFVEYLSQLKQTRIKHKLK
                     KRLRETM"
     gene            15967..16881
                     /gene="fmt"
                     /locus_tag="PKW44_00545"
     CDS             15967..16881
                     /gene="fmt"
                     /locus_tag="PKW44_00545"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013146915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HQR50108.1"
                     /translation="MKLIFAGTPEFAVPALAALIEAGHEVVLVLTQPDRPAGRGMQLK
                     ASPVKALAQQHGIPVYQPESLKTTDSRAPLAIAGADAMVVAAYGLILPQAVLDLPHHG
                     CLNIHASLLPRWRGAAPIQRAILAGDKETGVTIMQVVLALDAGAMLEKATLPITEHDT
                     AQTLHDALAKLGGRLMVETLAELETLTPQPQDEALVTYAEKLRKDEAPLDWRKPADEL
                     VRQVRAFNPFPVAQTVFKDETWRIWMAYAVPASAAPGVVVDTSQGIVVGCGEGALRIT
                     ELQKAGGKRLPARNFLAGNPVKPGERFA"
     gene            16941..17783
                     /gene="htpX"
                     /locus_tag="PKW44_00550"
     CDS             16941..17783
                     /gene="htpX"
                     /locus_tag="PKW44_00550"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HQR50109.1"
                     /translation="MFGNWLKTSILMAGIVVLFGMVGAALGGAGGMLIALLLAGAMNI
                     YAYWFSDTAVLKMYNAQEVDETSAPRFYGMIRELSQNGGLPMPKIYIIDEAQPNAFAT
                     GRNPEHAAVAATTGIMNLLSERELRGVMAHELAHVRHRDTLISTISATIAGAISSIAQ
                     FGMLFSGGDRERGAHPAVAILIMILAPVAAMLIQMAISRSREFEADRGGAEISGDPRA
                     LAAALEKIHNYAHQIPMQTAELHPETAQMMIINPLSAHGIQSLFSTHPRTEERVARLL
                     ELVV"
     gene            17796..19058
                     /gene="rsmB"
                     /locus_tag="PKW44_00555"
     CDS             17796..19058
                     /gene="rsmB"
                     /locus_tag="PKW44_00555"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HQR50110.1"
                     /translation="MDKSQQLAAHAVGQVLAGRNLTQTLESVWRNHPRLTPQQRAATQ
                     DLSYGALRHYGRLQALLDQLLQRTPDDPRVICLLLVALYQLEHGKASHYAVVDHAVKA
                     AAQLKPWAKGLVNAVLRNFLRQREPLLEKIAADEVARYSYPQWWIDKLRQQYPALWQS
                     ILEAGNQRPPMTLRVNRRHGSAEDYLAQLAESGISGRPLGDAAIMLEQPLAVEKLPGF
                     ATGAVSVQDYGAQFAASLLDVQNDMHVLDACCAPGGKTGHILEAAEVAMTAMDSDAGR
                     LARTRDNLERLRLSAALITGDAASPAGWWDGQPFDRILADVPCSASGIVRRHVDIKWL
                     RREIDLQTFSLQQAAILQGLWPMLAIGGKLLYATCSVFLEENQCQIDTFLRQHADARQ
                     LPMPGMANGQLLPCDQHDGFFYALLQKI"
     gene            19093..19605
                     /locus_tag="PKW44_00560"
     CDS             19093..19605
                     /locus_tag="PKW44_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HQR50111.1"
                     /translation="MTVSAHAAALQIKSAELVEAGEGYQLNADFDLNFSTEVEEALNK
                     GVPLNFLVEFKLVSPRRFWFDEEIVTATQQLRLSFHALSRQYLINLPQHQKSFASLPE
                     ALEALSRLRDWIVLEKSQVGKDEEYRAELRFWLDTERLPKALQVEALGSEKWNLSAKP
                     YRWTPGFKFE"
     gene            19602..21725
                     /locus_tag="PKW44_00565"
     CDS             19602..21725
                     /locus_tag="PKW44_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQR50112.1"
                     /translation="MKYVVFITATLGVVLLYLLSHASANTAVSGQRYTMLLVLNIALA
                     VFLTALIGTQLWKLYRQIRAQVIGSRLTLRLLGAFALMALIPGMIVYAVSVNFLTRSI
                     ESWFNVKVEAALDGGLRLGQSTLDIMLEDLREKGENIAISLAFQTPAAHLSLLHELRE
                     KSNVQDAVLLTPQGRILGVSSADSSGFLPELPTVSQLNQARSRTYGTIEPILGKGLYL
                     RVLSPVAASDMHGETRILQLLQPVPKALSSTAESVQQVYQDYQELSFTRSALKDVFAL
                     TLTLVMMLAMLSAVAFAFVLSRRLSAPLGLLAEGTRAIASGDYNMVLPAHGKDELGIL
                     VRSFNSMTQQLGEATRAAERNRARVEAARGHLQTILSHLSSGVLALDEKLQLRTWNPA
                     AAEILRLDLEQAVSQPPELLEQQSPVLTILFGTVRSHFETRLEGEWSQQVEIAGAHGM
                     QVLLLRGTRLPKGSESSYVVVFDDVTTLLQAQRDAAWGEVARRLAHEIKNPLTPIMLS
                     AERLEHKLSSKLDAADAAMLQRATHNIVSQVGALKSMVDEFSEYARAPALSLSKLDLN
                     QLVREVLALYEPHGIPLEAELQKAMPRIMGDSTMLRQVLHNLLKNAQDALEGREKPLI
                     RVKSACENGMVRLEVSDNGPGFPPDMMARVFEPYVTTKRHGTGLGLAIVKKIMEEHKG
                     TIKIENLPQGGAMVGLRLPLAGVSA"
     gene            21722..22981
                     /locus_tag="PKW44_00570"
     CDS             21722..22981
                     /locus_tag="PKW44_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HQR50113.1"
                     /translation="MSARQVLVVDDEIGIRELLYDILQDEGYHVKLAENAAQARDWRQ
                     QERPDLVLLDIWMPDCDGISLLKEWGNGGLLTMPVVMMSGHGTIDTAVEATRIGAFDF
                     LEKPIALQKLLKTVDAALRSSEQKPRTSAGLLNLGKSAVVTELRERLEQLSKRNGPLL
                     LIGAKGAGCELCARFLLQPDTPWLELTQTAKLATAPLDFLEQVRDGLIYIPEVADLGR
                     TEQKGLLVLLGKLDKYGTRVVCATARTLPQLAQSGQFDEALLQQLSGASLHIPTLAEH
                     REDIPDLAKSMTTLLAEGGEAPYRDFDVAALNALRNTEWPGNLEQLRGVVKNLMLTAL
                     GERITLEEVKRVLGQFAELAPQPIAAAPQLDLDLPLREARDAFERLYLEHHIAKASGN
                     MSKVAEAVELERTHLYRKLKQLGVKPK"
     gene            complement(23005..>23923)
                     /locus_tag="PKW44_00575"
     CDS             complement(23005..>23923)
                     /locus_tag="PKW44_00575"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HQR50114.1"
                     /translation="LMAREIMAAMVLPQLLMVCLAGIMVWYGVKRGLRPLDRLRREIG
                     QRSHLDLSPVPAESAPQEVQPLLLSMNDLMGRVRQTMELQQRFVADASHQLRTPLAGL
                     QTQAEMALRERDPGTVRHALERILASTVRLSHLVGQLLALARVEPGPGREASSMQPLD
                     LVRLAREVTAEWVSAALARDIDLGFECTEGAVMVSGDAVLLREMLANLLDNAIRYMAN
                     PGEITVHIVRESGRALLTVEDHGQGIPEAERARVFERFYRLSDSTGDGCGLGLAIVRE
                     IVLAHQATISITDTVSGRGTRVIVAFPTL"
BASE COUNT         5136 a         7065 c         6705 g         5017 t
ORIGIN      
        1 tacctctcgg catcacgcgt caattgcctt tgtctttatc cttgccagcc ggtgtgatct
       61 tgatattgtt gatggtcaca attctggaca gcttggaaat gctatatgca aaggcaccga
      121 gttggtagaa atcacccgtc aatttgatat tgatcggcat ctccgcatag aaatctttgg
      181 ggatctcttt tccaggcttg aacaattcga attccagccc ctgctccagt ccagcctggt
      241 tgatgtccgt caacaaggca tccatctgcg acttgttagg caactgtcgc aacagggcgc
      301 cgaaggtgcg ttcgatatcc accatctgtt gcctgtatgc cggcaggtta atcgcctctt
      361 ctttcttgac gaggaatacc ttgcgcaatt cagcttcttt ctgtctgctt atatcgagct
      421 cttcgtaagc cggcttccag aagacccaga aacctgctgt tacaatcagg ccaagggcta
      481 ttagcaacaa cactgccttg accggtaacg gcaggtaccc gagatttttc gggtcgattt
      541 tggtgagttc ttccagcttc atgctttatt tgcccttggg tttaaccggc tcaaccggtt
      601 tctgtacctt ttgcgtcacc tcgacactga aagaattttg cttggccccc ttggacggga
      661 ccgacttgat ttcaaccagc tgaggcgact caagccactc ggagttgctt aggttgcgca
      721 tcagcgccgc cacccgcgca ttgctgtccg cgacaccctc aataaccacc ttttcgtcct
      781 tctgctttac agctttaagg tacaagccct ccggttgctg tttggcaatt tcattaagca
      841 acatgacagc ttggctacga tcagtttgaa gggcttccac cgctttcttc cgcgcaagca
      901 cattgtcgat ttctgttctc agtcgcttga tttcttcgat ttctttgtca agcacgacaa
      961 tggcattgtc caagcgctta ttacgctcgt cttgcgattc tttcatgtca ttgatgtagg
     1021 tgagcccgag aaacacgatg gctgcaccgg cggcgacagt agccgcgaac ataaggctga
     1081 actggcgctg gagctcggcc cgtcggatct gacggtgtgg cagaagattg acgcgaacca
     1141 tcttttagtc gaacctcctc aaggccaatc cgcatgcaat catcaaagag ggcgcttccg
     1201 ccaacaggct ctgctgattc accctgttag aaaatgacat attgatgaag ggattggcga
     1261 ttatggtatt gacgcgaacc cggtctgcca ctgcctggtc tacgcccggg attgccgcac
     1321 atcctccggc cagcatcacg tggtcgatct ggttgaactg cgtggaactg gtaaaaaaat
     1381 gcactgctcg cgccacttcc atcgccaaag actgcacaaa cggctgcaac aactccggct
     1441 cgtaactttc tggcaagccg cccttgcgct tggcaatctc agcctcttct gcagacaaac
     1501 cgaagcgcct ctggatttcc agtgtcagct ggttgccgcc gaaattttgc tcgcggatgt
     1561 agacggattc gttattgtgc aagacattga cgtgcatcat gaaagcaccg atatccacca
     1621 ccataacagt ttgttcgcgg cccccttgcg gaagctgctt ggctgccaac tgataagcag
     1681 cttcagtagc ataagtttct acatccatga cgaccgcttt gagtccagcc gcctctaccg
     1741 aagccacgcg atcttcgatt ttttccttgc gcgacgctgc aatcagcacc tctacttcgt
     1801 cttcgctcga tgaagccggt cccaccacct gaaaatcgat attgacctcg tcaagcgaga
     1861 atggaatgta ctggttggct tctgcttcga cccgaagctc catgtcctct tcgcgcaaat
     1921 ccgcctgcat gatcaccttt ttgctgatca ctgccgccac gggcaacgcc agtgctacac
     1981 gtctctcgcg gctgccaagt agcttccatg cccgctttac ggcttcgccc gtttgctcca
     2041 gattggtaat attgccatct gtcaccgcgt ctttcggcag tggcgcaatg gcataaccct
     2101 caacccgata gccccccttg ctgccttcgg acaattccac catcttgatt gcggccgaac
     2161 tgatatcaac gccaatcagt ggcagttctt ttttcttgaa aaagtcgaat tccaaactga
     2221 aattcccttt ctgtactgat tagttacgcg caatacggat aatttacgtt aaatcctagc
     2281 aacaaccata ggccatgtaa agcattcttt acgatattaa tttaccatca ctcaagccta
     2341 taatacacgt tcattaattt ttctcattat ccacataatg tttccaaaaa agtggtggca
     2401 gttgctcttg ctgtccctgc tatccgccgg ggtagcggtt tccgcgctga tcgcattggc
     2461 agttgtgttg atttaccccg atttgccgtc gctagagacg ctgaccgact atcgtccaaa
     2521 gatcccgcta cgggtctatt ccgaagacgg ctacctgatc agcgaatttg gcgaagagcg
     2581 ccgcgctctg gtcaaaatca atgatgtgcc caaggaaatg aaggacgcca ttctggcaat
     2641 cgaggatcgg cgtttctatc aacacggcgg catagacacc gtcggcgtct tgcgtgccat
     2701 tctttccaac ctgacctctg gagatgcccg ggaaggcgcc agcaccatta ccatgcaggt
     2761 ggcccgcaat ttctttctgt ccagtgaaaa aaccctcaaa cgcaaggcgg gcgaggcatt
     2821 gctggcaatg aaaatcgagc agaatctgag caaggaccag attctcgagc tgtacatcaa
     2881 ccagatttat ctggggcagc gcgcttacgg cttttcggcc gccgctcaag tttatttcgg
     2941 caaaccgcta aacaagttga acctcgccga aatggcgatg ctggcggggc tgcccaaagc
     3001 gccatcacgc tacaaccctt ttaccaacat gaaacgtgcc cagtcccgtc agcaggatgt
     3061 attgcggcat atgtggcgct tcaacttcat tgacgaaaga acctaccagg cagcgctcaa
     3121 acagcctatc catttgaaag cgtcccgcca gtcgcgcgat cttgccgccg actacgctgc
     3181 cgagattgtg cgtcaacaac tatatgaccg ctatcaggaa agcatttaca gcagcggcct
     3241 caaggtatat accaccattc tcaaagctaa ccaggaggcg gctaacaaag ccgttctgca
     3301 aggtgttctg gactacgaca aacgtcacgg ttaccgcggc cctgaaggaa aaatcaatct
     3361 gcgagaggcc ccgcagggag cgatggaaac cttcctgcga gaggccatgg cggacgtcga
     3421 agaatacatg gagctggaac ctgcagtggt actggaagtc aatgccagca gcattaaagc
     3481 cttccgcaag aaaggcgaag tcatcaacat caatggcgat ggcttgctac tggtcaaaac
     3541 catgctttct gacaaaaatc cctccaagct gaagccacaa cctggcagtt tgatccgggt
     3601 aaaaaggcag ggtgataact ggcaaattgt ccagctgcca caggtcgaag ctgcattaat
     3661 tgctgtcgac ccaaagaacg gcgctgttcg cgccctggtt ggcggttttg atttcagccg
     3721 caacaagttc aaccatacca ctcaagcctg gcgccagcct ggatcgagtt tcaagccgtt
     3781 catctattcc gcggcactgg aaaaaggctt tacgccagcc agcgtcattg aggacgagcc
     3841 actggtcatt tccgcagagg aaacgggtag cggaaaggca tgggaaccgc ataatttcga
     3901 tgccaaattc gaagggccaa tgcgcatgcg cacggcgctt accaagtcca agaacatggt
     3961 ttccatccgt attctgcagg cgatcggcgt caattacgca caggactaca tcacccgttt
     4021 cggtttcgcg gccaaagacc atccggccta cctggcgatg gcgctgggcg ccggctcagt
     4081 cacgccctgg cagatggcgg gcgcgtacgc cacgtttgcc aacggcggct atcgcgtgac
     4141 gccttatctg atcaacaaaa tcgtcgacaa caacggaaag atcatggaac aagccaagcc
     4201 ggtcatcgcc ggagtaaatg cgcagcgggt gatagacccg cgcaatgcct tcatcatgac
     4261 ttcgatgatg caggacgttg cccgcatcgg cactgccgcc aaggctaggg agcttggtcg
     4321 ctacgatctg gcgggaaaga ctggcactac caacaaccac atggacgcct ggttcgccgg
     4381 ctacaatccg acccaggtcg ccgtcgcctg gatcggtttc gaccagcctc gctccctcgg
     4441 taaccgcgag actggcggcg cagccgcact accgatgtgg atcagttata tgtctcgcgc
     4501 actgcaaggc gtgcccgatg tggcctatca agtaccggac ggcatcgcat ccatcaagat
     4561 caacctggcc acaggagaac gggcgacgga ataccagggt ggcctgtatg aatattttta
     4621 cctggaaaat ccacccccgg ctgcagatgc caacagcgaa caggcagcag aaaccggtgc
     4681 caaggcagac gaggaacctg gtcccgaaca actgttctga agaagaactc atcaatgccc
     4741 tccaacatac gcccgcagca tttgcgccag atgatcgccc aactggccgc acgcatgatc
     4801 gcggaagatg gcgcacagga ctacgcccac gccaaacgca aggcagcacg acagctcgca
     4861 gtgagcgaca cccattgcct gcccaccaat agcgaaatcg agcaggaact caggctgcat
     4921 catgaaattt ttcgcagcgc cgagcaaccg cggcatctgt tccggctacg caacgaggca
     4981 ctggcagtga tgcgcttgct ggaacgcttc aaccctcatc tggtgggggc cgtgttggaa
     5041 ggcacggcgg ggcgttatgc ggaaacggaa atccacctgt ttgccgatag cctcaaggat
     5101 gtggagttgt tcctgctaaa ccaaggcatt cagtaccgga tggaagaaaa aacctaccgc
     5161 ttcggcaacg agcggcggtc attgccgcgg ttttcactgg aaggggcgca cggcgtcata
     5221 caagtcactg tttttgctac cgacgacctg cgcgtccctc ccaaaagccg actgcgcaat
     5281 ggatcacagg aacgtgccga cacaccaact gtggcggcaa tggttgccct aaacctttag
     5341 ccagcgggca acgtcccgcg caaagtaggt caggataccg tccgcgccgg cgcgcttgaa
     5401 tgagagcagg gcctccagca cgcaggcttt ttcgtccagc cagccattct gcgcggccgc
     5461 cttcaacatc gcgtactcgc cgctgacttg ataggcgaag gtcggtacac cgaattcctc
     5521 cttcacgcgg cggacgatgt cgagataggg cataccgggc ttgaccatca ccatatctgc
     5581 cccttcttcc agatccagcg cgacttcctt aagcgcctcg tcagaattgg ccgggtccat
     5641 ctggtaggtg gttttgtcgc ccttgcccag gttggcggca gagccgaccg catcgcggaa
     5701 cggcccatag aatgccgaag catacttggc ggaataagcc aggatacgcg tatggatgtg
     5761 accgttgaat tccagcgcct ggcgaatcgc cccgatgcgg ccatccatca tgtctgacgg
     5821 cgccaccaca tcagcaccgg cttgcgcatg gcacagcgcc tgctgcacca gcacggctac
     5881 ggtttcgtca ttcagcacgt agccgctctc atcggtcagg ccgtcctggc cgtggctggt
     5941 ataaggatcg agcgcgatat cggtgatgac cccaagttcc gggaatgcct gcttcaatgc
     6001 cctgatggtg cgcggtacca gaccgtcagg gttataggcc tcggcagcat ccagcgattt
     6061 ccgtgattgc tccaccaccg ggaacagcgc caatgccggg atacccagcc tgacgcactc
     6121 ttcggcggtt ttcagcagca catcaacgct ttgccgctgc acgcccggca tggaggcgac
     6181 ggcctcgctg cggctctcgc catccagtac gaacaccggg taaatcaggt cattggcagt
     6241 cagcacattc tcgcgcatca ggcggcgtga gaaatcatcc cgccgcatgc ggcgcggacg
     6301 ggtggaaggg tatttgccgt aagcgtggga catggggttt ccttatccaa aaacacgcgc
     6361 cagttcgacg cccgggtcgg gctggcgcat gaaagcctcg cccaccagga acgtgtggac
     6421 gttgtggcta cgcatcagcg ccacgtcttg cggcgtgaag atgccacttt ccgttacgac
     6481 gatgcgatcc tgtgggatgc ggctcaacag ccccagcgtg gtgtcgaggg taacatcgaa
     6541 ggtgcgcagg ttgcggttat tgataccgat cagtggcgtt tccagttgca acgcctgatc
     6601 aagttcctca ccgtcatgca cctccaccag caccgccatg ccaagatcgt gggcgatatt
     6661 ttccagttct ttcatgcggg gaagatccag cgcggcagcg atgagcagga tacagtcggc
     6721 acccatggcg cgcgcttcgt agacctggta tggggcgatc atgaaatcct tgcgcaatac
     6781 cggaatattg caggcacccc gcgcctgctt caaatattcc gggctgcccc ggaaatactg
     6841 cgtgtccgtc aacaccgata aacaggctgc gccatgggca gcgtatgtga cggcaatttc
     6901 ggccgggcgg aaatcctcgc ggatcacgcc cttggatggg ctggctttct tgatctcggc
     6961 gatcaccgca gcttggccgg cagcgatttt tgcacggatg gcgccaatga aatcgcgtgg
     7021 agcggtagcg ctttccgcct tgcttcgcac cagccccaaa ggctgtagca cgttggctgc
     7081 gttcacttcc tcctgcttgg tggccaggat ttttttcagg atgtcagaca tggttgttat
     7141 tggaaagagg gggcccagaa acaaatttga ggcttaattt taccttcgtc cagaatatta
     7201 aatcgacaat tttgcgtttg cagcaggttt gattgccggt tgtggctctc ccgccaccgc
     7261 gcggtgcgat acacctgcat gtgataaaat gagcgtcttg ataaaacccc tacagcagca
     7321 aattccctgg acaaaaatca agagtgaaac tgtgggagag gaaaccttca tgcgcgcatt
     7381 ccttttgagc atcctcctgt gtctggcact gaccgcatgc ggcgccaaag gaccgctcta
     7441 tctgcccgaa aaacaatacc cgcaaggcaa ccaatgaatc ctttcgagtc aataaacggc
     7501 gaattgcacg ccgaaaatac gcctttgtcg caaatcgcag cgcaattcgg cacgccttgt
     7561 tatgtctatt cccgcaccgc gctgacagag gccttccaac gcttcgatgc cggtttcagt
     7621 ggtcatgacc acttggtctg ctacgcggtc aaggccaacc ccaacctcgc tatcctcaac
     7681 ctgttcgcca ggctgggcgc gggtttcgac atcgtttccg gcggcgagtt ggcacgtgtg
     7741 ctggcagccg gcggcgatcc tggcaaggta gtgttctccg gcgtcggcaa gacggccgcg
     7801 gaaatgcgcg cggcgctaca ggccgggatt ttttgcttca acgtagagtc ttcggcggaa
     7861 ctggagcgac ttaacactgt ggcaggtgag atgaacaagc gcgcgcctgt gtccttgcgt
     7921 gtcaatccga acgtggacgc caagacacac ccttacattt ccactggtct caagaacaac
     7981 aaattcggcg ttgcctacga ggaagcattc gcgctctacc agcgcgccgc agacttgcca
     8041 aatatcgaaa tccacggcgt ggattgccat atcggttcgc agctcaccga attatcgccg
     8101 tttctggatg cgctcgaccg cgtgctggcg ctggtagacc agatggaaag ttccggcatt
     8161 caccttcgtc acctggatat tgggggcggc atcggcatcc gctatgccga cgagacgcca
     8221 cccgattttg ccaactatag ccgcgccatt ctggacaagg ttggcgggcg taacttcaag
     8281 ctggtgatgg agcctggacg tgcactggta ggcaatgccg gcttgctgct gaccacggtg
     8341 gaatatctca agcccggcga gagcaaaaac ttcgctattg tagatgctgc catgaacgac
     8401 ctgatgcgcc ccgcgctgta cgacgcctgg catgaaattc tgccggttcg cgcacggcag
     8461 gacactgagc aaatctacga aatcgtcggg ccagtatgcg aaagcggcga ttttctcggc
     8521 cacgaccgca agctttccct acagcagggc gacctgcttg ctatcctgtc tgccggcgcc
     8581 tatggcatga gcatgagttc caactacaac acccgcccgc gcgccgcaga agtcatggtg
     8641 gacggccgcc agctgcatct ggtgcgcgag cgcgaaactc tgctacagct tttttcgctc
     8701 gaacacattc ttacaacctg acgtttattt cctaatcaaa aatcaccgtc ttgttggcat
     8761 acagcaggat gcggctttcc acgtgccagc gcactgcgcg tgacagtacc accttctcca
     8821 gatcgcgacc tttctggatc aggtcgtcga tctggtcgcg atgcgagacg cgcatcaggt
     8881 cttgctcgat gatcgggcct tcgtccagca cctctgtgac gtaatggctg gtggcgccga
     8941 tcagcttgac gccgcgttca aaagcacggt gataggggcg ggcaccgatg aacgccggca
     9001 ggaaggagtg gtggatattg atgatgcgtt gcgggtagcg ccgcacgaac tcgggcgaca
     9061 ggatctgcat atagcgcgcc agcacgatca ggtcaatctg gtgctggtca aacagcgcga
     9121 attggcgggc ttccgcctcg gtcttgctgt ccttggtcac ttcgatgtga tggaacggga
     9181 tgccgtagaa ttccgccagc cgctgcgtag ccttgtggtt gctgatgatg agcgggatgt
     9241 cgcaagccag ctccccgctc tggtggcggt agagcagatc gaccaggcag tggtcataat
     9301 gcgacaccat aatggccatg cgcgggcgct gttgcgataa cttgagttgc cattgcatct
     9361 ggaaacgctg cgccaacggt gaaaaatgtt cggcaaaggt ttccggtgca ataccgaaac
     9421 cgttcagatc ccattccacg cgcatcagaa acagattgtt ctcggcgtcc tggtgctggt
     9481 cggcgtgcag gatattggca tcgtgccggt acagaaaatc ggcgatggca gcgaccagcc
     9541 ctttgcgatc cgggcaattg attagcagag tggcggtatt tttcatggct ccaaccgagg
     9601 acacgacaaa ggcggcaatt ataccgattc catgttgaaa tctggcggtt tgtggtatct
     9661 tttcccacct gatccgcaac agaaccatat cgacaatccc gtgaaaaaac agtggcctta
     9721 tttcgttctg gcgtttgcca tccccatctt gctggtgttg tggtggtggg gattgttcac
     9781 cacggcaacc atcgaagtca cgacactcgg accctaccgc tacgcctatc tggaagccca
     9841 gggcgcatac tccaaacttg ccggcaagca gcgtgaggtc atgttcgaac tacagaagca
     9901 aggtattacc ccggaaggaa gcattaccct gatcctggac gatccacgca ccaccccata
     9961 tgacaagctg gtggcccgga ccggctatca aatcgccaca tcggccacgc ctgcctcacc
    10021 gctggcgctg gaaaccatcc cggcacgcga agtcgtggtc gcacaagtca gagcgcaccc
    10081 gcttttcgcc tacggcaagg cctattccgc attgctggat tacacccggc aacacggtat
    10141 ggcgttgcgc atgcctacgc tggaacgcta cagcaacagc gtgctgaccg tcgaaatgcc
    10201 tgtggaaaga tcaccatgag cttttttgtc ctgctcgctg ctctggtact aaaccattac
    10261 cggccactgt cccggacaga cctgctgcaa gcagtgttcg gcccttacgc caacctgctg
    10321 gaacgcaact tcaacgatgg tcaggcgcgg catggtctgg tcgcatggct actggccata
    10381 ctaccgccgg tattgctgat cggcgccgtc tatttcgtac tgtaccggct caacatcttg
    10441 cttggcatgc tgttcgccat cgccgtgctc tatgttacgc cgcgcttcgg gcgtttcagt
    10501 acgccacctg aacagattgc tgccctgctg catgaaggca ggctggacga cgcacgtgaa
    10561 cggctgcgcc aatggcagtc attagacacg catgactatg atgcgggaca ggtcgcgcgc
    10621 gtcagcatcg aatcaacgct gtgtcaggca caccacgact tcatcgcccc cgtattctgg
    10681 ttcgtcctgc tcggcccggc tggggcagtg ctttaccgac tggcgcatct gttgagaaat
    10741 gtctggggga acaagtccgg agccttcggc gatttcagca aacgggcttt cgactggctc
    10801 gactggcttc ccgcccgctt taccgccggt tgttttgccg tgatgggaga tttcgaagac
    10861 gccgtttact gttggcgcat acaggccttc gcctggcctg accgtgccgt cggcatcatg
    10921 cttgccagcg gcgctggtgc cttgggcgta cgtctgggcg cggcgatacc gcgtgacggc
    10981 aacatggaat accgacccga gcttggtttg ggcgacgaag ccgatgccga ctacctacat
    11041 gccgccagcg gcttggtgtg gcgtgtgctc gccctgatac tggggttgct gttgttgttg
    11101 acatttgcac attggctagg aaagtaatct gccaaaatga aagttttact ggcaaaccca
    11161 cgcggctttt gtgccggcgt cgatcgtgcc atcattatcg tggagcaggc actggaacgc
    11221 ttcggcgcgc cgatttacgt gcgccacgag gtcgtgcaca acaagttcgt ggtggacgag
    11281 ctgaagcgca agggcgcagt gttcgtcgag gaacttgatg aggtgcccac tggtaacacc
    11341 gtcattttca gcgcgcatgg agtttccaag gatgtgcgcg ctgaagcgga ggtacggggc
    11401 ctgcgcgtat tcgatgccac ctgcccgctg gtgaccaaag tacatgtcga ggtcgccagg
    11461 atgcgtgctg ctggcaggga aattatcatg atcggtcaca aggggcaccc ggaagtcgaa
    11521 ggcaccatgg gacagtccga gggcggcatg tacctcgtgg aaacgccgga agatgtcggc
    11581 aggctgcagg tggctgatcc agacaagctc gcctatgtca cccagaccac tctgtcgatc
    11641 gacgatgccg ccgcaatcgt ggatgcgctg aaggcgcgct tccccggcat cgccgctccc
    11701 aagagcgatt ctatctgcta cgccacccag aaccggcagg atgccgtcaa gacgctggcc
    11761 catggctgcg atctagtgat cgtggtcggc tcgcccaata gttccaactc caaccgcctg
    11821 cgggaagtgg cacgcaatca gggcgtagag tcctacatgg tggataacgc cagccagttg
    11881 caactggaat ggctggctgg caagcagcgg gtcggcgtca ctgccggcgc ctccgcaccc
    11941 gaggtgctgg tgcaggaagt gattgcgcgg ttgagcgccc tcggtgccag cgacgtgcac
    12001 gaactcgaag gcatcatcga acatgtggta ttccccttac caaaaaatct ggctgcatga
    12061 gcaaaacatt cacccttgcg ctgaccggcg cctccggtat gccgtatgcc atgcgcctgc
    12121 tggaatgcct gctcggcgct ggccacaccg tgtatctggt ctactcccag gccgcccagt
    12181 ttgtcgctgc ccaggaaatg gcgatcagcc tgccggccaa gccttctgag gcacatcgac
    12241 tgctggtgga gcgcctgcat tgccgccccc agcagttact ggtttttgcg cgtgaagact
    12301 ggaatgcgcc catcgcctcc ggtagtggcg tggccgacgc catggtggtc tgtccttgta
    12361 ccatgggctg cctcgcggct attgccaacg ggctctccga caacctgatc gaacgggcag
    12421 cggacgtgat gctgaaggag gggcgcaagc tgattctggt acctcgcgaa acgccgtttt
    12481 ccgccatcca tctggaaaac atgctcaagc tggcgcgact cggcaccgtg atcctgccgc
    12541 ccaacccggg cttctaccat cagcccgtgc atttggacga tctggtggat ttcgttgtcg
    12601 ccaggatact cgatcagctt ggtgtggagc accggttggt caagcgctgg ggcagcgaca
    12661 acgcgcaatg ctcttgaaaa cgagcacctt gttagtgcat ggtcggacgc atatcgctga
    12721 acaccgcgtc ctctacaaac aggtaatcgc gcgccttgcc ctgattccac atggtcatca
    12781 gcataatcca cttgagctgt tccagcgtca ccttgccgtc cggcaacgcc atggcacggt
    12841 cgatcgccag ttcccgctga gccgcatcaa tgacacccgc ctgttccagg aaaaccaaga
    12901 agccttggca ttcgagattg atctttcggc tttccacatc cgcgtagata cgcagaccat
    12961 tgcccccttc atggcagctt gcagccatat ggctggtcat ctcttcaagt gtggtgaacc
    13021 aatcgaatgc gctgctgatg tcgttttcgt caaacccagc ggcgaacagc tcacgtgcca
    13081 gcgtatcctg atcggggcga atattggctt cgaaataatt ttcgaacaag tagacgagga
    13141 cttcaaacat acagtctccc cattaattca atagttaact gatgcgctgg tagcgtcctc
    13201 cgggcagaca agccactttg ccctccagtt ccagtattag cagcatggcg gaaagatttt
    13261 cgctcgtcaa gccgctgcgc gcgaccagtt catccatgcc aatcggatcg taacccatat
    13321 gaccgagcag gggctcatca gcttgggcaa gtgccacatc ttctgcccgc ggcacggcaa
    13381 cgccaagttc ttccaggata tcctgcatgc tttccaccag ctttgcccct tgcttgatca
    13441 acaaatggca acccttggcc accggcgaat ggatggagcc ggggatggcg aatacctcgc
    13501 gcccctgttc agctgcgagc cgcgcggtga tgagtgagcc actttgtaaa tgcgcttcca
    13561 ccaccaggca gccttggctc aaaccgctaa tgatgcggtt gcggcgcgga aaattttgcg
    13621 ccatggatgg cgtccccagc gggaactcgg aaatgagcag gccgcgttcc gatatctggt
    13681 gtgccagctg ccgatgacgt gccggataaa caatatcgag gccggtaccg acgacggaaa
    13741 tggtactagc gcgaccctgc agcgcgccac gatgggccgc cccgtctatc ccaagcgcca
    13801 ggccgctgac gatacagtag ccgttgtcgc ttagcacctg cgagaacgcc tcggcggtct
    13861 tttccccctg tggcgtggcg ttgcggctac cgacaacggc cagcgacggc tgactaagca
    13921 actcgattct gcccttggca tacatcaacg gcgggggatc tgaaatttcc agcaacgcct
    13981 tcgggtaggc ggtgtctgcc aacgtgatca gatgattgcc gggttgttcc aaccactcca
    14041 ggacgggacg gatggatgcg tggtcaaggc cggcggcaat ctgttgcgcc accggccccc
    14101 ccaccacttt atgcagctcc gcataaggaa tggcataaat ccgttcgggc gtgccgaaag
    14161 cggaaagcaa ggcgcgcagt ccttgccgcc ccaagcccgc gatcaggctc aaccccaccc
    14221 actgcgccgc atcttctggc gttgtgccgg gcaaagcctc tgccattacg gggtcggggt
    14281 tcgcacgacg tccagcacat tcaccggcct ctccgccttc atgatgatgg cgtaagacac
    14341 gcgtttgaag gcgcggaaca ccatgagcaa gccgatacgc tcatccggaa gcaacagggg
    14401 tttaggcttg tctccctttt ttgaagctgg cgcgggtacc accgtgcccg gccggtatat
    14461 ggccagcacc tcgccttcgg ccagtccgtc atcactacca cgattgatga tcacgacgga
    14521 gttcggccca agctcggcaa tcgcattgta ggccgatata atgcggccgg cgatatcact
    14581 ttccggtgcg cgcggcacaa aactgttcat gtcggtatca ggcattttga acagtttgtc
    14641 acccttgtaa acttcctctt tgggacgaac gatttgaatg gtggccggat ccccatatct
    14701 cacggcgcgc gcatcgccga ggtataccgt ctcgaacccc agttgttcct tggtgtcggg
    14761 atcaaccagt gcattcccct tgcggaagat ctgccagttg agtccaccac cttcctcgat
    14821 atggtcaaca tagatgttgt atccagaagt aagcgccacg tgtccccctt ccgctccgac
    14881 gatgctgggg gcctgttgta attcttcctc actgactatc aagggctgag tgagaaaagg
    14941 ggcaatagcg ccaggcgaaa tcgttgggat gggcttcttt tccaacggtt ctaccatcgt
    15001 gcctggttcc agcgtgatgg tttcgcgcaa caggcgcaac tggggctgac cattgctgtc
    15061 gacatcgaga actacaacgt ctccagggta aatcaggtga ggattcttga tctgttcacg
    15121 gttcattttc cagatctgtg gccagcgcca cggatctttc aggaaccttc ctgctatccc
    15181 ccaaagcgtg tcgcccttga ccacgacata tcggtcggga tggccatcct gcaactggat
    15241 attgtcggct cgcgccactg gcgcaaggca gcaaagtaac agcagcgcta taatatattt
    15301 gttcatattg gcctcaacct gtagcagcgg aaccctgatt atcgcgaagt tggtttaaat
    15361 tctgcatgta attcattcaa caatcaagct tttatggcca tattaagtat tctgcaatat
    15421 cccgacgagc ggctgcatac ggtggcacag cctgtgacgg aagtcgaggc ccgggttcgc
    15481 cgcctggttg cggacatggc ggaaaccatg tacgccgcgc ccggcatcgg tttggcggct
    15541 acccaggtca atgtgcacca gcaggtaatc gtgatcgaca tttccgagga aaaaaaccag
    15601 ctgatggtgt tcatcaatcc tcgtattgtg accagcggca gcatgcgaga ctatgaggag
    15661 ggctgcctgt cggtccccgg catttacgaa aaggtacgcc gtccggaaca ggtgacggtc
    15721 gaggcgctag atgcggacgg caaaccgttc acccggcaag ccgatggctt gctggcagtc
    15781 tgcattcagc atgaaatgga ccacctcaag ggcaaggtat tcgttgaata cctctcgcaa
    15841 ctcaaacaga cgcgcatcaa gcacaaactg aagaagcgcc tgcgggaaac catgtagtac
    15901 ttgaccgagg ccgagtgaaa gcgaagttgt tgtcgcttgc atcctgcttg tactgaagga
    15961 tattgaatga agctgatttt tgccggtacg ccggagtttg ccgttcctgc tcttgctgca
    16021 ctcatcgagg ccggccatga agtcgtacta gtacttaccc agccggatcg gccggccggt
    16081 cgcggcatgc agctcaaggc cagcccggtg aaggcgctgg cgcagcaaca cggcatcccg
    16141 gtctaccagc cagaatccct caagacgact gacagccggg caccgctggc aatcgcaggg
    16201 gctgacgcca tggtcgtcgc cgcctatggg ctaatcctgc cgcaagccgt gcttgacctc
    16261 ccgcaccatg gctgcctcaa tatccacgcc tcgctgttgc cgcgctggcg cggcgcagcg
    16321 ccgatccaac gcgctattct ggcgggagat aaggaaaccg gcgtcaccat tatgcaggtg
    16381 gtactagcgc tggatgccgg cgccatgctg gagaaagcaa cccttcccat taccgagcac
    16441 gatactgcac aaaccctgca cgacgccttg gccaagctgg gcggcagact aatggtggaa
    16501 acgctggccg agttggagac gcttactcct cagccgcagg acgaggcgct ggtgacctac
    16561 gccgaaaagc tgcgcaagga tgaagcgccg ctggactggc gtaaaccagc ggacgaactg
    16621 gtgcgtcagg tgcgcgcgtt caaccccttt ccagtggcgc aaacggtgtt caaggatgaa
    16681 acctggcgca tctggatggc gtacgctgtt cctgcttcag ccgcgccagg cgtggtggtg
    16741 gatacttcac aagggatcgt ggtcggatgc ggcgagggcg ccctgcgcat tacggaattg
    16801 cagaaagccg ggggcaagcg actaccggca cggaatttcc tggccggcaa cccggtaaag
    16861 ccgggcgagc gcttcgcttg aatttttagc agtggacgcc atctaacgtg gcgttcccta
    16921 tcgtctgata aggttagaaa atgtttggaa actggctgaa aacttccatt ctgatggccg
    16981 gcatcgtcgt gctatttggt atggtgggcg cggcgctggg tggcgcaggc ggcatgttga
    17041 tcgcactgct attggccggg gcgatgaaca tctacgcata ctggttctcg gacactgccg
    17101 tgctaaaaat gtataacgcg caggaggtcg atgaaaccag cgcccccagg ttctacggaa
    17161 tgatccgcga actgtcgcag aatggcggcc tgcccatgcc caagatctat atcatcgacg
    17221 aggcacagcc caacgccttc gccaccggac gcaacccgga acatgctgcg gtggccgcca
    17281 ccaccggcat catgaacctg ctgagtgagc gtgaactgcg tggcgtgatg gcgcatgaac
    17341 tggcacatgt caggcatcgc gacacgctga tatcgactat ttccgccacc atcgctggcg
    17401 ctatttcctc tatcgcccag ttcggcatgc tgttctccgg cggcgatcgc gagcgcggcg
    17461 cgcatccggc agtcgcaata ctcatcatga tcctggcacc ggttgccgcc atgctgatcc
    17521 agatggcgat ttcgcgttcg cgcgagttcg aggccgaccg tggcggtgct gaaatcagcg
    17581 gcgaccctcg ggcgctggcg gcggcactgg aaaaaatcca caactacgcc caccagattc
    17641 ccatgcaaac tgccgaactg cacccggaaa cagcacaaat gatgatcatc aatccgttat
    17701 cggcgcacgg gatccagagt ctgttcagca ctcatccgcg caccgaggaa cgcgtcgccc
    17761 gcctgctgga gctggtagtt tgacaggctt acgtcttgga taaaagccag cagcttgcag
    17821 cccatgccgt cggccaggtg ctggctggtc gcaatctcac ccagacactg gaaagtgtct
    17881 ggcgcaatca tccccgtctc accccgcaac aacgagccgc cacacaggat ttgagctacg
    17941 gtgcgctgcg ccactacggg cgcctgcagg cgctactcga tcaactgctg caacgcacgc
    18001 cggatgaccc gcgcgtgatt tgtctgctgc tggtggctct gtaccaactg gagcacggca
    18061 aggcctcgca ctatgcagtg gtggatcacg cggtcaaagc cgccgcccag ttaaaaccgt
    18121 gggcgaaggg cctggttaac gccgtgctgc gcaacttcct gcggcaacgt gaacccttgc
    18181 tggaaaaaat cgccgccgat gaagttgccc ggtattccta tccacaatgg tggatagaca
    18241 agctcagaca gcagtacccg gcgctctggc agtccattct ggaagctggc aaccagcgcc
    18301 cgcctatgac tctgcgcgtc aaccggcggc acggaagcgc tgaggactat ctggcacaac
    18361 tggccgagtc cggcatctcc ggccggccgc taggcgatgc tgccatcatg ctggaacagc
    18421 cgctggcggt ggaaaaactg ccgggattcg ctactggcgc ggtttcggtg caggactatg
    18481 gcgcacagtt tgccgcgtcg ctgctggatg tgcagaatga catgcacgtg ctggatgcct
    18541 gctgcgctcc gggcggcaag accgggcaca tcctggaagc ggccgaagta gcaatgacgg
    18601 cgatggatag cgatgccggg cgcctggccc ggacccgcga caatctggag cgcctgaggc
    18661 tgtcagccgc actcatcact ggcgatgctg ccagccccgc cggctggtgg gacggccagc
    18721 cgtttgatcg catcctggca gacgtgccat gcagcgcctc cggcatcgtg cgtcgccacg
    18781 tagacatcaa gtggctgcgg cgcgaaatag atttgcagac cttttcctta cagcaggctg
    18841 ctattctgca aggattgtgg ccgatgctgg caatcggtgg taaattgctg tacgcgacct
    18901 gttccgtttt tcttgaagaa aatcaatgtc aaatagatac cttcctacgg caacatgcag
    18961 atgcgcgcca actgcccatg ccgggaatgg caaacggaca gttgcttccc tgtgatcaac
    19021 atgacgggtt tttctatgcg ctactgcaaa aaatttagcc gattcatcct gttgatgctg
    19081 gtgttgtgcg ctatgactgt cagcgctcat gcagctgccc ttcagatcaa gagcgccgaa
    19141 cttgtcgagg ccggggaggg ctatcagctc aatgccgatt tcgacctcaa cttcagcacc
    19201 gaggtagagg aagcactcaa caagggcgta ccgctcaatt ttctggtcga attcaagctg
    19261 gtatcgccac ggcgcttctg gttcgatgag gaaatcgtca ccgccaccca gcagctccgc
    19321 ctgagtttcc atgccctgtc gcgccagtac ctgatcaatc tgccgcagca ccagaaatcg
    19381 tttgccagcc tgccagaggc actggaggcg ctgtcacgcc tgcgcgactg gatcgtgttg
    19441 gaaaaatcgc aggtcggaaa agacgaggaa taccgcgctg aattgcgctt ctggctcgat
    19501 accgagcgcc tgcccaaggc gctgcaggtc gaagccctgg gttcggaaaa atggaacctg
    19561 tccgcaaagc catatcgctg gacacctggc ttcaagtttg aatgaaatac gtcgttttca
    19621 tcaccgccac cctaggggtg gtactgcttt acctgctatc ccacgccagt gccaacaccg
    19681 ctgtatccgg ccagcgctat accatgctgc tggtcctcaa cattgcgttg gcggtattct
    19741 tgaccgcact gatcggcacc cagttgtgga agctttaccg ccagatccgc gcgcaggtca
    19801 tcggcagccg tctgaccctg cggctgctcg gcgccttcgc cttgatggcg ctgatccccg
    19861 gcatgatcgt atatgcggta tcggtcaatt tcctcacccg ctccatcgaa tcctggttta
    19921 acgtcaaggt cgaggccgcc ctggatggtg ggctgcgcct aggccagagc acgcttgaca
    19981 tcatgctgga agacctgcgt gaaaaagggg aaaacatcgc catctcactt gcctttcaga
    20041 cgccagccgc ccatctttcc ctgctgcacg agctgcgcga aaaaagcaac gtgcaggatg
    20101 ccgttctgct gacgccacaa ggacgtattc tgggagtttc cagcgccgat tcgtccgggt
    20161 ttttgccaga actccccacc gtgtcgcaat tgaatcaggc acgcagccgc acttacggca
    20221 ccattgagcc catcctggga aaaggtttgt atctgcgggt gctgtcgccg gtagccgcca
    20281 gtgacatgca cggcgaaacg cgcatcctgc aattgctgca gccagtaccg aaagccctgt
    20341 cgagcaccgc cgaatcggta cagcaggtct atcaggacta tcaggagctt tcatttacgc
    20401 ggagcgcgct gaaagacgtg tttgcgctga ccctgacgct ggtgatgatg ctggccatgt
    20461 tgtccgccgt ggcgtttgcc ttcgtgttga gccgccgtct ctccgccccg ctcggcctgc
    20521 tggcagaagg tacgcgcgcc attgccagcg gcgattacaa catggtgctg ccggcgcacg
    20581 gcaaggacga gctgggcatc ttggtgcgct ccttcaacag catgacacaa cagctgggcg
    20641 aagccacccg ggccgcggag cgcaaccgcg cgcgcgtaga ggcggcgcgc ggccacctgc
    20701 aaaccatcct ttcacacctt tcctccggcg tgttggcgct ggacgagaag ttgcagttac
    20761 gcacctggaa ccccgccgct gcggaaattc tccggctcga cctggagcaa gcggttagcc
    20821 agccgccgga attgctggaa cagcaatctc cagtcctgac gattttattt ggcacggttc
    20881 gctcacattt tgagacgcgc cttgaaggag aatggagtca gcaggtcgaa atcgccgggg
    20941 cgcatggcat gcaggtgctg ctgctgcgcg gcacgcgcct gcccaagggc tcagaaagca
    21001 gctacgtggt ggtattcgac gatgtgacca ccttgctgca ggcgcaacgt gacgcagcct
    21061 ggggagaagt ggcgcggcga ttggcgcatg aaatcaagaa cccgctgacc cccatcatgc
    21121 tttccgccga gcgactggag cataaattga gcagcaagct ggatgccgcc gatgctgcca
    21181 tgctgcagcg cgccacccac aatatcgtga gccaggtagg tgcactgaaa agcatggtgg
    21241 acgaattcag cgaatacgcc cgggcacctg cgctcagctt gtccaaactt gacctcaatc
    21301 aactggtacg ggaagtgctt gcgctgtacg aaccacatgg cattccactg gaggcagagt
    21361 tacagaaagc tatgcctcgg atcatgggcg attccaccat gctgcgtcag gtgctgcata
    21421 atttgctgaa aaatgcacag gacgccctgg agggtcggga aaagccgctg atccgggtga
    21481 aaagtgcatg tgaaaatggt atggttaggc tggaggtttc cgataacggt cctggctttc
    21541 cgccggacat gatggcccgt gtattcgagc cttacgtcac taccaaacgc catggtaccg
    21601 ggctcggact tgccatcgtg aagaagatca tggaagaaca caaaggtacg atcaagatag
    21661 aaaacctgcc gcagggcggc gcgatggttg gtctgcggct gccgctggcc ggggtgtcag
    21721 catgagcgca cgccaagttc tggtggttga cgacgaaatc ggcatccgcg agttgctgta
    21781 cgacatcctg caggacgagg gctaccacgt caaactagcg gagaatgcag cgcaggcgcg
    21841 cgactggcgg cagcaggaac gcccagatct ggtgctgctt gacatctgga tgcccgattg
    21901 cgatggcatc agccttctca aggaatgggg taatggcggt ctgctgacga tgccggtagt
    21961 gatgatgtca gggcatggca ccattgacac ggcagtggaa gcgacccgca tcggcgcctt
    22021 cgacttcctg gaaaaaccga tcgcgctgca aaaactgctt aaaacggtag atgccgcgct
    22081 caggtcttca gagcagaaac cccggaccag tgccggcctg ctcaacctgg gcaagagcgc
    22141 agtagtgacc gaactacggg aacgcctgga acaactcagc aaacgcaatg gcccattgct
    22201 gctgatcggc gccaagggtg ccggctgcga actatgcgct cgcttcctgc ttcagcctga
    22261 tacgccatgg ctggaactga cccagacggc caaactggca actgccccac tagatttcct
    22321 ggagcaggtg cgcgacggcc tgatctatat tcccgaggtg gccgatctcg gcagaaccga
    22381 acagaaaggc ctgttagtgc tgctgggcaa gctcgacaag tacggcacgc gcgtggtgtg
    22441 cgccaccgcg cgcacgcttc cgcaactggc gcaaagcggg caattcgacg aagcattgtt
    22501 gcaacaattg tcgggtgcca gcctgcatat cccgacgctg gcggaacacc gcgaggatat
    22561 tccagacttg gcaaaatcca tgaccacgct actagcggaa ggcggcgagg ctccctaccg
    22621 cgacttcgat gtcgcagccc tcaatgcctt gcgcaacacg gagtggccgg gcaacctcga
    22681 acaattgcgt ggggtggtga aaaacctgat gctgacagcc ttgggcgaaa gaatcacact
    22741 ggaggaagtc aaacgggtac tgggtcagtt tgccgagctg gcgccgcaac ccattgcggc
    22801 cgctccgcaa ctggatctgg atttgccttt gcgcgaggcg cgagacgcat tcgagcgtct
    22861 gtatctggag caccacatcg ccaaggcgag cggaaacatg agcaaggtgg cggaagcggt
    22921 ggaactggag cgtacccacc tgtaccgcaa gctgaagcaa ttgggtgtga aacccaaata
    22981 aacctagcaa cagcggttgt tcggtcaaag ggttggaaat gcaacaatca cccgggtgcc
    23041 gcgcccgctg accgtatcgg tgatactgat cgtggcctga tgtgccagaa caatctcgcg
    23101 cacaatagcg agccccagcc cacagccatc gccggtacta tccgacaggc gataaaagcg
    23161 ctcgaaaaca cgtgcgcgtt ccgcctcggg gatgccttgg ccatgatctt ctaccgttag
    23221 caaagcccgc ccactttcac gcacgatatg cacggtaatc tcgccaggat ttgccatata
    23281 gcgaatcgcg ttgtccagca aattagccag catttcgcgt aatagcaccg cgtcgccgga
    23341 caccatcacc gccccctcgg tacattcaaa acccagatca atgtcgcgtg ccagcgcagc
    23401 tgatacccat tcggcggtaa cttcgcgcgc caatctcacc aaatcgaggg gttgcatgct
    23461 ggaagcttca cgtcccggac ccggttcgac ccgtgccagc gcaagtagct gaccaaccag
    23521 gtgtgacagg cgtacggtac tggcaagaat gcgttccagc gcatggcgta cggtgccggg
    23581 gtcacgctcc cgcagcgcca tctcggcttg cgtctgcaag ccggccagcg gggtgcgcag
    23641 ttggtgcgaa gcatcggcga cgaagcgctg ctgcaattcc atggtctgcc ttacccgtcc
    23701 catcagatcg ttcatggaga gcagcaaggg ctgcacttcc tgcggcgcgc tttccgctgg
    23761 gaccgggctc aaatctagat gggaacgctg gccgatttcg cgtcgcaacc tgtccagcgg
    23821 acgcaggccg cgcttgaccc cgtaccagac catgatgccg gccaggcaaa ccatgaggag
    23881 ctgcggcaac accatggcag ccatgatttc cctcgccatc agt
//
LOCUS       DAOMFA010000005        22220 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_131_length_22220_cov_5.808031, whole genome shotgun sequence.
ACCESSION   DAOMFA010000005 DAOMFA010000000
VERSION     DAOMFA010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 22220)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22220)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22220
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_131_length_22220_cov_5.808031"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(109..1053)
                     /locus_tag="PKW44_00580"
     CDS             complement(109..1053)
                     /locus_tag="PKW44_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HQR50115.1"
                     /translation="MKAALRCMTAILLVLPLLALAEETIPYLLTAASKGDLQMVNAML
                     KSGVNPNTKDADGITALMYAARKNSAPVAKALLDKGADVNAKDEGGWTALMFAARKNH
                     VETVKVLLEGKADISVRDPSGWSALGLAATSGYSEVVGLLVKHGANASAKSDDGRSIL
                     MYAAKNGDVPTIKILLENGADLSARDRQGVTAAMYAAQNGNVDALQTLLDGGAKVDEK
                     DSSRWTALTWAVKKNQLAVAQLLVAKGADVNVRDIEDTPLLKKAVDSGSVEMVKLLID
                     NKANVKAKDKYGLTALVYAMKDGQPEMVKLIKAAGGSY"
     gene            complement(1050..1937)
                     /gene="sucD"
                     /locus_tag="PKW44_00585"
     CDS             complement(1050..1937)
                     /gene="sucD"
                     /locus_tag="PKW44_00585"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HQR50116.1"
                     /translation="MSILVNKNTKVLCQGFTGKQGTFHSEQALAYGTKMVGGVTPGKG
                     GQTHLGLPVFNTMRDAVRATGANASVIYVPPAFAADSILEAADAGVELVVCITEGIPV
                     LDMLNVKAALKANGTRMVGPNCPGVITPDECKIGIMPGSIHRRGKVGIVSRSGTLTYE
                     AVHQTTQLGLGQTTCVGIGGDPIKGLNFIECLQLFQADPETEAIIMVGEIGGSDEEAA
                     AEYIQSHVSKPVAAYIAGQTAPAGKRMGHAGAIISGGSGKASDKIRALEKAGVAVASS
                     PAGLGEAVQAAIKSGGRKI"
     gene            complement(1946..3106)
                     /gene="sucC"
                     /locus_tag="PKW44_00590"
     CDS             complement(1946..3106)
                     /gene="sucC"
                     /locus_tag="PKW44_00590"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019899350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HQR50117.1"
                     /translation="MNLHEYQAKHLLQQYGLPTPRGIVVDAAEAAVAAARELGGEAWV
                     GKAQIHAGGRGKAGGVKVTRSHDALQNLATQLLGSQLVTLQNAPDGQPVHRLLIEETL
                     PIARELYLSVLVDRSVERVVVVASAAGGMEIEEIAHESPDRILREVCDPLLGLVDYQA
                     RSLAFALGLAGEQIAAFTKLLKGLYRLFRENDLSMLEINPLVVTVTGQLVALDCKMSV
                     DDNALYRQKVLAEQRDWSQDDAKEAEAHAAGLNYIALNGNIGCMVNGAGLAMATMDLI
                     KLHGGMPANFLDVGGGATAETVAKAFKIILADSNVKAILVNIFGGIMRCDIIAEGIIT
                     AVRDVGVKVPVVVRLEGTNVELGKQMLRDSGLSIISADGLTDAAQRAVAAVV"
     gene            complement(3192..4571)
                     /locus_tag="PKW44_00595"
     CDS             complement(3192..4571)
                     /locus_tag="PKW44_00595"
                     /inference="COORDINATES: protein motif:HMM:NF015669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HQR50118.1"
                     /translation="MKSSAISLFFLLVTQEVLGNGLVTGTAAYRERIAMPPDAVFEAT
                     LEDVSLADAPAKTIGQVRIEALGSPPIHFEIPYDEARIEARHSYAVRARVLRSDGLMF
                     FTDRSYPVLTRGHGKQVSMMMHLNPGPGSGKNATDEQVIGPLPASFAGDLPCADCQGI
                     RYQLDLFADHAFFLRMSYMGKNRAFDDIGTWSLADGRTLILDGGREARVRFSVKEAAT
                     LRKLDQEGREIESALNYDLKREATLQPIEPQIFMGGMYHYMADAGIFTECLTRRRMPV
                     AQVQDNAALERDYVNLRREPGEEIMATLEGRLVMRPKMEGEGLQQVLIVERFIKLWPG
                     ESCGAQFSTASLENTYWKLTRLGDVPVTVNEGQREPHLLLRREGRHVGGYGGCNRLMG
                     RYELNGVEITFGNMAMTMMACPSGMDTEQAFHKALAHVKSWKITGEHLELLDAGGVSL
                     ARFEAIYMK"
     gene            4850..7537
                     /gene="aceE"
                     /locus_tag="PKW44_00600"
     CDS             4850..7537
                     /gene="aceE"
                     /locus_tag="PKW44_00600"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019899379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HQR50119.1"
                     /translation="MANDLGEHLIMASPIPDIDPQETQEWLDAIDAVLEQEGTQRAHF
                     LLETLIDKARRSGAYLPYNATTAYVNTIPVHQQAKLPGDPSMERRIRALIRWNAIATV
                     MRANEKSPGVGGHIASFQSAATLYDVGFNHFFRSPEHPSGGDLVLFQGHSSPGIYARA
                     FIEGRISEEQMANFRQETGGNGLSSYPHPWLMPDFWQFPTVSMGLGPLQGIYTARFLK
                     YLHDRGIADTSQRKVWVFCGDGEMDEPESLGAISLAAREKLDNLIFVINCNLQRLDGP
                     VRGNGKIVQELEAAFRGSGWNVLKVLWGSYWDPLLAMDSNGLLKKRMEEAVDGEYQNF
                     KAKGGAYTREHFFGKYPELKEMVAAMSDQDIWRLNRGGHDPHKVFAAYDAATKHVGQP
                     SVILAKTVKGYGMGAAGEAQNPTHQAKALDMDSLRAFRDRFEIPVSDAELEQLPFYRP
                     AEDSPEMQYLRERRNALGGYVPSRRRKGNTLETPPLSAFDNMLGATGDREISTTMAFV
                     RILSTLVKDKNIGKYVVPIVPDEARTFGMEGMFRQLGIYSSVGQLYEPVDSDQVMFYK
                     ESKSGQILEEGINEAGAFSSWLAAATSYSVTGVQMIPFYIYYSMFGFQRIGDLAWAAG
                     DSRARGFLLGATAGRTTLNGEGLQHEDGHSQLMAAMIPNCVSYDPTFAYELAVIMQDG
                     LRRMVQEQEDVFYYLTVMNENYSHPEMPKGAEEGIIKGLYQFSKSKAKGPKVQLMGSG
                     VILREVIAAAELLEKDWGVSADIWSAPSFNELRRDGLDAHRWNMLHPDKPQKESHVEK
                     CLKKVEGPVIASTDYMKEFADQIRAFVPQRYVALGTDGFGRSDSREALRKHFEVDRHY
                     IVLAALKALADDGKLPAAKAAEAIKKYGIDINKPNPLMA"
     gene            7551..8885
                     /gene="aceF"
                     /locus_tag="PKW44_00605"
     CDS             7551..8885
                     /gene="aceF"
                     /locus_tag="PKW44_00605"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HQR50120.1"
                     /translation="MAIKEVLVPDIGNFDSVDVIEVHIKTGDSVNQDDSLITLESDKA
                     SMDIPSPFSGTVKEVKIKVGDKAAQGTLVALIEAAEGAAAVEAPKPAAESAPAAAPVA
                     AAVAEIPPPSRPVPEVPKPIAPQGTPNPVAAHAEFAPGKNPHASPSVRKFARELGADL
                     TRVTGSGTKGRISKDDVQAWVKGELTKPRAEAAAGGGLAVLALPSIDFSQFGPVETQT
                     LPRIKKISGANLHRNWVTAPHVTQFDNADITDLEDFRKSMQDEANKRGVKLTTLAFLM
                     KAVVNALRTYPNFNASLSPAGDELILKQYYNVGFACDTPDGLVVPVIRNVNEKDVLDI
                     ARDLGELSAKARERKLKVEEMQGGCFTISSLGGIGGTAFTPIINCPEVAILGVSRSSI
                     EPVWDAKSKTFEPRLMLPLSLSYDHRVIDGADGARFTSHLRMMLSDVRRLLL"
     gene            8898..10676
                     /gene="lpdA"
                     /locus_tag="PKW44_00610"
     CDS             8898..10676
                     /gene="lpdA"
                     /locus_tag="PKW44_00610"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HQR50121.1"
                     /translation="MSQTIEIAVPDIGNFDSVDVIEVHVKVGDSINVDDSLITLESDK
                     ASMDIPSTHAGTVKAVTVKVGDKVAKGTAILTLEALAGETTPTAQPAAPLAAALASAT
                     AQATAPTPAAATAGPSDLHAEVLVLGAGPGGYTAAFRAADLGKKVVLIERYPSLGGVC
                     LNVGCIPSKALLHAAKVITEAEEMVEHGISFGKPNIDIAKLKAWKEKDVVGKLTGGLG
                     ALAKQRNVTVVNGRGEFSSTNQIKVTASDGKVTTISFDYAIIAAGSQATKMPGLAPEH
                     QNDPRILDSTSALALEDIPKRLLVVGGGIIGLEMGTVYDAFGSKVSVVELSDGLIPGC
                     DRDLVKPLHKRMERRFEKILLKTKVAKLEPKKDGIHVTFEGDNAVEPQVYDRVLVAIG
                     RRPNGRNIGAEKAGVFVNEYGFIPVDKQQRTNVPHIFAIGDIVGQPMLAHKAVHEGKV
                     AAEVIDGHKVEFQALAIPSVAYTDPEVAWAGISETDAKAKGIEVEKASFPWVASGRAL
                     SIGRTEGSTKLIMEKGSRRIIGAGIVGPNAGELLAEAVLAIEMGADAEDIGLTIHAHP
                     TLSETVCFAAEMAEGTITDLLPPRKK"
     gene            complement(10728..11384)
                     /locus_tag="PKW44_00615"
     CDS             complement(10728..11384)
                     /locus_tag="PKW44_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020181692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HQR50122.1"
                     /translation="MLNSRTVLFDLDGTLVDTAPDLALALNMQRERHGLPPLPLETIR
                     PYASHGSRGLLGVGFGLAPGHAHFSTMREEYLDLYDQVFTRSPVMFNGIAEILETMEN
                     EGMAWGVVTNKPRRFTQPLMLALGLTERAACIVSGDDATRPKPHPDTLLLACDLAKIH
                     PEACCYVGDAERDIIAGNTAGMRTIVACYGYLDANDKPQEWGASAMIDSPAELLALLR
                     "
     gene            complement(11378..12100)
                     /gene="ubiG"
                     /locus_tag="PKW44_00620"
     CDS             complement(11378..12100)
                     /gene="ubiG"
                     /locus_tag="PKW44_00620"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008689 - 3-demethylubiquinone-9
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HQR50123.1"
                     /translation="MASQAETVSPLNADPAELQKFAALAHRWWDPTSEFKPLHDINPL
                     RLDYIDHVAGLAGKRVLDVGCGGGILSESMAQRGAEVTGIDLGEKALKVAQLHKLESG
                     AMVNYRLVAVETLADEKPASYDVVTCMEMLEHVPDPATVVRACARLVKPGGRVFFSTL
                     NRNLKSYLLAVIGAEYVLNLLPKGTHEYQKFIKPSELSAWCRTAGLEVSGITGMNYNL
                     FSKRYTLGEDVSVNYIIHTIKS"
     gene            complement(12067..13401)
                     /locus_tag="PKW44_00625"
     CDS             complement(12067..13401)
                     /locus_tag="PKW44_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRZ/ATZ family hydrolase"
                     /protein_id="HQR50124.1"
                     /translation="MPEAHINTVDLLIEARWIIPVMPRGVVLENSAIAIDQGRVMAIL
                     PIAEARQCFHALETVSLPTHVLIPGLINLHTHAAMSLMRGLADDVPLMTWLDAHIWPA
                     EVELVSARFVHDGTLLACAEMLRGGVTCFNDMYFFPQAAAEAVERSGIRACLGLVLLE
                     FPSGYAADADDYLSKGLAARDALRGRERITTCLAPHAPYTVSDRSFGKLLTYADQLGL
                     NLHTHLHETREEISQSEAQYGLRPLQRLTGLGLLGPNLLVTHCVHLTLGEIETLSAQG
                     CHVAHCPSSNLKLASGIAPISTLLAAGINIGIGTDSAASNNRLDMFSEMRLAALLAKA
                     AGDASTLKAGQALEMATLNGARALGLDDHIGSIEPGKLADLAAVDFSSPEMQPCFDPL
                     SHLIYVAGREQVSHTWVAGELRYQRGVHDSVDIAELQEITTRWQARLKQFHH"
     gene            complement(13463..14623)
                     /locus_tag="PKW44_00630"
     CDS             complement(13463..14623)
                     /locus_tag="PKW44_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HQR50125.1"
                     /translation="MKNKLIGLAVAGAFAMSAGAAFAADEFEGSWYVMPNIGMMHADS
                     DLDAESWAPAYGISLGKQLSEHWDVQIGGTYAKADSDKSGVGGDYKQTLFGVDALYLF
                     SREKFRPYLLAGLGYAHNDVDYNANGTNYGDSNNSVYGKLGFGFQYSFTDRIGLQFGL
                     REVWSRADVPQSYTGANNTDSKTVGNLYTNLGLIINFPPPPKAVVVAAPVAAGLPEVA
                     PVEDEVYEFTVEETVVEEGPAKPAVKVTLAAEVLFGFDNATLKDEGKSLLNTEVVEKM
                     KAHPEAELVLVTGYTDRIGDAKYNQKLSERRANAVKKYLVSQGIEENRLHAVGKGEAD
                     PIAECKGVRGKKLIECLQPNRRVVVEVERQRAVEEVTVEQTEVQGVVVEPQQ"
     gene            complement(14699..15466)
                     /locus_tag="PKW44_00635"
     CDS             complement(14699..15466)
                     /locus_tag="PKW44_00635"
                     /inference="COORDINATES: protein motif:HMM:NF016367.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(5-formylfuran-3-yl)methyl phosphate synthase"
                     /protein_id="HQR50126.1"
                     /translation="MELADGKNRQLQGRHARGEPGVTRLLASVTSAEEAMIALEAGAD
                     IIDLKNPAQGTLGALPLDVIRAIVKVVDGRRPVSATIGDLPVQPSLLAGRVAETASTG
                     VDIIKVGFFGNTGHQEAVRALTPLVAMGACIVAVLFADHRPDMKLLPLLENAGFYGVM
                     LDTENKNGGRLTDYLGLDALHDFVIASRSKRMQVGLAGSLMESDIASLSLLKPDYLGF
                     RGALCANHERKAMLDRTHLESVQSMLRECNKLAVDAH"
     gene            complement(15400..15975)
                     /locus_tag="PKW44_00640"
     CDS             complement(15400..15975)
                     /locus_tag="PKW44_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF447 family protein"
                     /protein_id="HQR50127.1"
                     /translation="MINETIITSSDADGKVHIAPFGVRERDGLVLIAPFRPSSSLDNL
                     LHGRCAVVNATVDVRIFAGALTGRRDWPVRRANKIDGWVLDAALTHRELELVDVQEDA
                     VRPELLFRVVHEAIHAPFRGFNRAQAAVLEAAVLVSRLHLLPLEKIEHEVNYLTIAVE
                     KTAGERELEAWSWLMEKIDNYKAGMRGENLA"
     gene            complement(15972..17372)
                     /locus_tag="PKW44_00645"
     CDS             complement(15972..17372)
                     /locus_tag="PKW44_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6513 domain-containing protein"
                     /protein_id="HQR50128.1"
                     /translation="MSENILFLTGKLAESSLHKVLAGMQPAQFDYQVAQLGISVAALM
                     TPEFIARRLPDARGAGRIVLPGLCRGNLVPLQEKYGVPVQLGPEDLKDIPQYFGHGGK
                     EPDLSNYDVKIFAEIVEAPQLPVADILARAERYRAQGANVIDLGCLPNTPFPHLEDAV
                     QALKAAGMQVSIDSMQQEELLRGGRAGADYLLSLTEKTLWIADEVTAMPVLVPARPGS
                     LPSLYRAIEAMQKKGQAFIADPILDPIPFGLAESIVRYHRLRKKHPDIQIMMGVGNLT
                     ELTDADTTGINAVLFGVIAELNLNAVLTTSVSPHACNAVREADVARRVMHAAHEDRRL
                     PRDYSNDLLGLHDRRPFPYSEAEIAELAAQVKDPNFRIQVSEQGVHIYNRDGLLCDID
                     PFRLYPYLKVDDDASHAFYLGVELARAQVAWQLKKRYSQDAELQWGCNSAEPDARGRV
                     AHREASLKKKRDMSGK"
     gene            complement(17348..17899)
                     /locus_tag="PKW44_00650"
     CDS             complement(17348..17899)
                     /locus_tag="PKW44_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HQR50129.1"
                     /translation="MALKQEKQRLAWAITGSGHYLRECLDIIGKLQDVDLFLSKAAAE
                     ILQQYGYAHSIGRVFQDKTASAVPVELFYHGVYHTVVIAPTSSNTVAKMVCGISDNLV
                     TNIFAQAGKGRVPTIVFACDTEPELESEAPREHMVKVYPRRIDLENMEKLKTFEETTV
                     AADMVELDAAIQARLACLKTSSF"
     gene            complement(17901..19214)
                     /locus_tag="PKW44_00655"
     CDS             complement(17901..19214)
                     /locus_tag="PKW44_00655"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HQR50130.1"
                     /translation="MNKNVVVIGTQWGDEGKGKIVDWLTDHAHGVVRFQGGHNAGHTL
                     VIGNKKTVLHLIPSGILRESVQCFIGNGVVLSPQALLTELRMLEEAGVNVRSRLRISE
                     ACPLILPYHVALDQAREAAKGAAKIGTTGRGIGPAYEDKVARRAIRLQDLFYRERFAA
                     KLGEVLDYHNFVLKNYFHAETVDFQRTLDDTLALAEQIKPMVADVSNLLYLANQAGQP
                     LLFEGAQGALLDVDHGTYPFVTSSNTIAGGATTGSGVAPQMLSYVLGITKAYTTRVGS
                     GPFPTELYDAVDKLDPIGQQLAKRGNEFGSTTGRARRCGWFDAAALKRSIQVNGVSGL
                     CITKLDVMDGMETVQIGVGYKIGGVTHDIFPVGAEALEHCEPIYEELPGWSESTFGIK
                     RYEDLPENAKRYLRRMEEVCGVPVDIISTGPDRDETIVLRHPFGA"
     gene            complement(19222..20394)
                     /locus_tag="PKW44_00660"
     CDS             complement(19222..20394)
                     /locus_tag="PKW44_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020181683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HQR50131.1"
                     /translation="MRNWLLPEYIEDVLPAEAARMEQVRRRLLDLFRVHGYQLVIPPL
                     LEYLESLTTGAGHDLDLATFKVVDLLSGRLMGIRADITPQAARIDAHLLNWQGVTRLC
                     YAGSVLRTRPDGLAQTRQPLQIGAELFGHAGIESDIEIQQLLVEALQAVGVQALQLDF
                     SHVGIFRSLVKRSGIATALEQDLLMALQSKGKAELNALTAGLDAEIRQALLALTELNG
                     GAEVLLAAEKSLPQYAEIKQALQDSRRISQQLSRLDVEISFDLAELRGYHYHSGLVFA
                     AYAQGHSGPLAQGGRYDEVGAIFGRARPATGFSLDLRGLTTALPPPPSQAAILAPFRE
                     DAGLQEAIAALRAAGEIVVVELPGHEAYRNELGCDRALTNKQGTWKVVPVAEFESR"
     gene            complement(20399..20584)
                     /locus_tag="PKW44_00665"
     CDS             complement(20399..20584)
                     /locus_tag="PKW44_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HQR50132.1"
                     /translation="MDNSLIAAFALMLVLEGILPLLAPQAWRQTFRRMTEFSDGQLRF
                     VGVLSMAGGLLLLLVTR"
     gene            complement(20591..21466)
                     /gene="hflC"
                     /locus_tag="PKW44_00670"
     CDS             complement(20591..21466)
                     /gene="hflC"
                     /locus_tag="PKW44_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HQR50133.1"
                     /translation="MKNLGSLLAGLLIALVLLSMSVYTVDQREFALVFRLGEIVAVKK
                     EPGLYFKIPLMENVRYFEKRIVTLDWVEPDRFITSEKKNVLVDSFVKWRITDPAKYYV
                     SVRGDERQAEARLSQTVNDGLRAEFGKRTIHDVVSGERDQIMEILRQKADREARQMGI
                     EVLDVRIKRVDLPEEVSGSVYQRMEAERKRVANELRSQGAGAAEKIRADADKQREVII
                     AEAYREAQKTKGEGDAKAAEIYASAYGRNPEFYAFYRSLEAYRNSFTNKSDVLVLQPD
                     SDFLKYMRDADGRRK"
     gene            complement(21463..>22220)
                     /gene="hflK"
                     /locus_tag="PKW44_00675"
     CDS             complement(21463..>22220)
                     /gene="hflK"
                     /locus_tag="PKW44_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HQR50134.1"
                     /translation="AIQYNLKSANDYLFNNRFSDQSYGNPEASVVRGAAETAIREVVG
                     KSKMDFVLYEGRAEVAARVKKLMQDILGRYQSGINIVNVTMQNAQPPEQVQAAFDDAV
                     KAGQDLERQKNEGQAYANDVIPKARGTAARLLEEAAGYKLRVVNESQGNASRFSQILS
                     QYEQAPQVTRQRLYLDAQEQILSNVSKVVVDQKGNNSLLYLPLDKLLANTGTAAPSAP
                     PATGPAATGPEVLDGGAQRSREAFRSRERGGRP"
BASE COUNT         4564 a         7022 c         6210 g         4424 t
ORIGIN      
        1 ttcacacggg caccccattc catgccaaaa acttggtaga agcgccgcct tcggcgcgac
       61 aagaatcggg aaatcgcgtc gaagcggcgc tcctacgaga gatcatggtc agtatgaacc
      121 gccggctgcc ttgatcagct tgaccatttc cggttgccca tccttcatgg cgtagaccag
      181 cgcggtcaga ccatatttgt ccttggcctt gacgttggcc ttgttgtcga tcagcagctt
      241 gaccatttcc acggagccac tgtccaccgc ctttttcagc agaggggtgt cctcgatatc
      301 ccggacattg acgtccgcgc ccttcgcgac cagcaactgc gccacggcca actggttttt
      361 cttgactgcc caggtcaggg ccgtccagcg cgaactgtcc ttctcatcga ccttggcgcc
      421 accgtccagc agcgtctgca aggcatcgac attgccattc tgggcggcgt acatggccgc
      481 cgtcacaccc tgacggtcac gcgccgacag atcggcaccg ttttccagta ggatcttgat
      541 cgtcggcaca tcgccgttct tggcggcgta catcaggatg ctcctgccgt cgtcgctctt
      601 ggcactggca ttagcgccat gcttgaccag cagccccacc acttcgctgt agcccgaggt
      661 cgccgccagg ccgagcgcgc tccagccgga ggggtcccgc acgctgatgt ccgccttgcc
      721 ttccagcagt accttgacgg tttccacatg gttttttctg gccgcgaaca tcaacgcagt
      781 ccaaccaccc tcgtccttgg cgttgacgtc ggcacccttg tccagtaggg ctttggccac
      841 cggggcgcta ttcttgcgcg cggcgtacat caaggcagta atgccatcgg catccttggt
      901 gttggggttc accccgcttt tcaacatggc gtttaccatc tgcaagtcgc ccttggaggc
      961 tgccgtcagc aggtaaggga tggtttcctc ggccaacgcc agcaaaggca atacaagcag
     1021 aatggccgtc atgcaacgca atgccgcttt catatttttc gccctccaga cttgatcgcc
     1081 gcttgcaccg cctctcccaa tccggccgga gagctggcca ctgcgacgcc ggccttttcc
     1141 agcgcgcgta tcttgtcgga ggccttgccg ctgcctccgg aaatgatggc gccggcgtgc
     1201 cccatgcgct tgcccgcagg cgcggtctgg ccagcaatat aggctgccac cggcttgctg
     1261 acatgcgact ggatgtactc tgccgccgcc tcctcgtcac tgccaccaat ctcgcctacc
     1321 atgatgatgg cctcggtttc cgggtcggcc tggaacagtt gcaggcactc gatgaaattc
     1381 agccccttga tcgggtctcc gcctatgccg acgcaagtgg tctgccccag ccccagttgc
     1441 gtggtctggt gcaccgcttc ataggtcagc gtgccggaac gcgagacgat gccgaccttg
     1501 ccccgccgat ggatgctgcc gggcatgatg ccgatcttgc actcgtccgg ggtgatgacg
     1561 ccgggacaat tgggcccgac cattctggtg ccgttggctt tgagcgccgc cttgacgttg
     1621 agcatatcca gcaccgggat gccttcggtg atgcagacta ccagttcgac acccgcatcc
     1681 gccgcttcca ggatggagtc cgccgcgaag gccggcggca cgtagatcac gctggcgttg
     1741 gcgccggtag cgcgtaccgc gtcgcgcatg gtgttgaata ccggcaggcc gagatgcgtt
     1801 tgcccgccct tgcctggcgt cacgccgccg accattttgg tgccataggc caatgcctgt
     1861 tcggagtgga aggtaccctg cttgccggta aagccctggc acaggacttt ggtgttttta
     1921 ttgaccagta tgctcatgtg aattcctaaa ccactgccgc cactgcgcgc tgggcggcgt
     1981 cagtcaaccc gtctgccgaa atgatggaaa gcccgctgtc acgcagcatc tgtttgccca
     2041 gctccacgtt agtgccttcc agtctaacca ccaccggcac cttgacgcca acgtccctga
     2101 ccgcggtgat gatgccctcg gcgatgatgt cgcaacgcat gatgccaccg aagatgttga
     2161 ccagaatcgc cttcacgttg ctgtcggcca gaatgatctt gaacgccttg gcaaccgtct
     2221 ccgcggtggc accgccacca acgtcgagga agttggccgg catgccgcca tgcagcttga
     2281 tcaggtccat ggtcgccatc gccagcccgg cgccgttgac catgcagccg atgttgccgt
     2341 tgagtgcgat gtagttcagg ccggcggcat gcgcctcggc ttccttggcg tcgtcctgac
     2401 tccagtcgcg ctgttcggcc aaaaccttct ggcggtagag tgcgttgtcg tccacactca
     2461 tcttgcagtc cagcgccacc agctgcccgg tgacagtcac caccagggga ttgatttcca
     2521 gcatgctcaa gtcgttttcc ctgaacagcc ggtacaaccc cttgagcagt ttggtgaagg
     2581 cagcaatctg ctcgccagcc aagcccagcg caaaggccag gctgcgtgcc tgatagtcca
     2641 ccaggcccag cagcgggtcg catacttcgc gcagaatcct gtcgggggat tcgtgggcga
     2701 tttcctcgat ctccatgccg ccggcggcgg aagccaccac caccacgcgc tcgacgcttc
     2761 gatccaccag cacgctcagg tacaactcac gcgcgatcgg cagtgtttcc tcgatcagca
     2821 gccgatgcac cggttggcca tccggtgcat tctgcaaggt caccaattgg ctccccaaca
     2881 actgcgtcgc cagattctgc aacgcgtcgt gcgaacgcgt caccttgacg ccacccgctt
     2941 tgccgcgtcc accggcgtga atctgcgcct tgcccaccca ggcttcgccg cccaactcgc
     3001 gcgccgcagc tacggccgct tctgcggcat ccaccacgat gccgcgcggc gtcggcaaac
     3061 catactgttg cagcagatgc ttggcctgat attcatgcaa attcatggtc gcccacccag
     3121 aaatgaattg gaggtcatta tagccgcaaa ggtggtgttc tcatgcccgg cacaatcaaa
     3181 aaccaacatc ctcacttcat gtagatcgcc tcgaagcgtg ccagcgatac accgccagca
     3241 tcgagcagtt ccagatgctc gccggtgatt ttccatgact tcacatgtgc cagcgccttg
     3301 tggaaggcct gttcggtgtc catgccggaa gggcaagcca tcatggtcat ggccatgtta
     3361 ccaaaagtaa tttccacgcc attcagctca tagcgcccca tcagccggtt gcagccacca
     3421 tatccgccca cgtgccgacc ctcccgcctt agcagaagat gcggctcgcg ctggccctcg
     3481 ttcacagtca ccggcacatc gccgagccgc gtaagtttcc agtaggtatt ctccaggctg
     3541 gccgtagaaa actgcgcacc acagctttca cccggccaca atttgataaa gcgctcgacg
     3601 atcagcacct gttgcaatcc ttcgccttcc attttcggcc gcatcacaag ccggccttcc
     3661 aacgtcgcca tgatttcctc gcccggctca cggcgcaagt tgacataatc gcgctccagc
     3721 gccgcgttgt cctgtacctg cgccaccggc atgcggcggc gggtcaggca ctcggtaaag
     3781 ataccggcgt cggccatgta gtgatacatg ccgcccatga aaatctgggg ctcgattggc
     3841 tgcagcgtgg cttcacgctt caggtcgtaa ttgagcgcgg actcgatctc ccgcccctcc
     3901 tggtccagtt ttctcagcgt cgctgcctcc ttgacggaaa atctgaccct ggcttcgcga
     3961 ccgccgtcga ggatcagcgt cctgccatcg gcaagtgacc aggtgccgat atcgtcgaaa
     4021 gcgcgattct tccccatgta ggacatgcgc aggaagaaag cgtgatccgc gaacaggtcg
     4081 agttgatagc gtataccctg gcaatcggca cagggcaggt caccggcaaa gcttgccggc
     4141 aacgggccga taacttgctc gtctgtggca tttttgccgg aacctggccc gggatttaag
     4201 tgcatcatca tcgacacctg cttgccgtgc ccgcgagtaa gcaccggata gctgcgatcc
     4261 gtgaaaaaca ttaggccatc actccgcaac acgcgggcac gtaccgcgta actatgcctg
     4321 gcttcgatgc gcgcctcgtc ataaggaatc tcgaaatgga tgggtgggct gcctagcgcc
     4381 tcaatacgca cctggccgat cgtcttggcg ggagcatccg ccagcgaaac atcctccagt
     4441 gttgcctcga acaccgcatc cggcggcatt gcgatgcgtt cacggtaggc ggcggttccc
     4501 gtaacaagtc cattgcccaa tacttcttgc gtaacaagca gaaaaaataa cgaaattgcc
     4561 gatgatttca tttgcagaaa accccttgtc cagccggttg tgagtccatg cagagtttgc
     4621 agggcatagt ttacccggta ctgtttccat acgcttgagt accgggctta cctccgaaaa
     4681 cgatacggcc cagtatcgat gggctttcct ggccaataag cagcttgcaa caggcgcttc
     4741 caagcttgac caaaacggtc aaactagcga agatagcgcc ttcgcatgca gcccggggct
     4801 gcaacaattc agccgaattc gccatcaacg gccctgtgat gccgaaacta tggcgaacga
     4861 cctaggagag cacctgatta tggcttcgcc catccccgat attgatccgc aggaaacgca
     4921 ggaatggctg gacgccatcg atgccgtact ggaacaggag ggcacccaac gtgcccactt
     4981 cctgctggaa accctgatcg acaaggcacg ccgctccggc gcctacctgc cgtacaacgc
     5041 caccacggcc tacgtcaaca ccatcccggt gcaccagcag gccaagctgc caggcgaccc
     5101 gagcatggag cgccgcatcc gcgcgctgat ccgctggaac gccatcgcca ccgtgatgcg
     5161 cgccaacgaa aaatccccgg gcgtgggcgg ccatatcgcc agcttccagt ccgccgccac
     5221 gctgtacgac gtcggcttca accatttctt ccgctcgccg gaacaccctt ccggcggcga
     5281 cctggtgctg ttccaggggc actcctcgcc cggcatctac gcccgcgcct tcatcgaagg
     5341 ccgcatcagc gaagagcaga tggcaaactt ccgccaggaa accggtggca atggcctgtc
     5401 ctcctacccg cacccgtggc tgatgccaga cttctggcag ttccccaccg tgtccatggg
     5461 cctcggcccg ctgcagggca tctacactgc ccgcttcctc aaatatctgc atgaccgcgg
     5521 catcgcagat acctcgcaac gcaaggtttg ggtattctgc ggcgacggcg aaatggacga
     5581 gccggagtcg ctgggcgcca tttcactggc tgcgcgcgaa aagctggaca acctgatttt
     5641 cgtcatcaac tgtaatctgc aacgtctcga cgggccggta cgcggcaacg gcaagatcgt
     5701 gcaggagctg gaagccgcct tccgtggctc cggttggaac gtgcttaagg tactgtgggg
     5761 ttcctactgg gatccattgc tggcaatgga cagcaatggc ctgctgaaaa agcgcatgga
     5821 agaagccgtg gacggcgaat accagaactt caaggccaag ggcggcgctt atacccgcga
     5881 acatttcttc ggcaagtatc cggaactgaa ggaaatggtg gccgccatgt ccgaccagga
     5941 catctggcgc ctcaaccgcg gtggccacga tccgcacaag gtgtttgcgg cctatgatgc
     6001 tgcaaccaag catgtcggcc agccgagcgt gatcctggcc aagaccgtca agggctacgg
     6061 catgggtgcc gccggtgagg cgcagaatcc gacccaccag gccaaggcgc tggatatgga
     6121 ttcactgcgt gccttcagag atagattcga gattccagtc tccgacgccg aactggaaca
     6181 gctgccgttc taccgcccgg cggaagacag tccggaaatg caatacctgc gcgaacgccg
     6241 caacgcgctt ggcggctacg tgccgtcgcg ccgccgcaag ggcaacacac tggaaacccc
     6301 gccgctgtcg gcgttcgaca acatgctggg cgccaccggc gaccgcgaga tttccaccac
     6361 catggccttc gtgcgcatcc tgtccacgct ggtcaaggac aagaacatcg gcaagtacgt
     6421 cgtgcctatc gtcccggacg aagcccgtac cttcggcatg gaaggcatgt tccgccagct
     6481 gggcatctat tcttccgtcg gtcagttgta tgagcccgta gactccgacc aagtgatgtt
     6541 ctacaaagaa tcgaagagcg gccagatcct ggaagaaggc atcaacgagg ccggcgcgtt
     6601 ctcgtcctgg ctggcagccg cgacctccta tagcgtcacc ggcgtgcaga tgatcccgtt
     6661 ctatatctac tactcgatgt tcggcttcca gcgcatcggc gacttggctt gggcggcggg
     6721 cgactcgcgc gcgcgcggct tcctgctggg tgctactgcc ggccgtacca cgctgaacgg
     6781 cgaaggcctg cagcacgagg acggccacag ccaactgatg gcggccatga tcccgaactg
     6841 cgtgtcctac gatccgacct ttgcctatga actggcggtg atcatgcagg acgggctgcg
     6901 ccgcatggtg caggaacagg aagacgtgtt ctattacctc acggtgatga acgaaaacta
     6961 cagccacccg gaaatgccca aaggcgctga ggaaggcatc atcaagggcc tgtaccagtt
     7021 cagcaaatcc aaggccaagg gcccgaaggt gcagttgatg ggcagcggcg tcatcctgcg
     7081 cgaagtcatt gccgccgccg aactgctgga gaaggactgg ggtgtttccg ccgatatctg
     7141 gagtgcgccc agcttcaacg aattgcgtcg tgacggcctg gatgcgcacc gctggaacat
     7201 gctgcacccc gacaaaccgc agaaggaaag ccacgtcgag aagtgcctga agaaggtcga
     7261 aggtccggtc atcgcatcga ccgactacat gaaagaattt gccgaccaga ttcgcgcgtt
     7321 cgtgccgcaa cgttacgtcg cactgggcac cgacggcttc ggccgttcgg attcgcgcga
     7381 ggcgctgcgc aagcacttcg aagtcgaccg ccactacatc gtgctggcgg cgctaaaggc
     7441 actggccgac gacggcaagc tgccggccgc caaggccgcc gaagccatca agaagtatgg
     7501 catcgacatc aacaaaccca accccctgat ggcttaagga gaacgagaac atggcaatca
     7561 aggaagtgct cgttcccgat atcggcaatt tcgacagcgt cgacgtcatc gaggtgcata
     7621 tcaagactgg cgacagcgtc aaccaggatg actcgctgat taccctggag tcggacaagg
     7681 cctccatgga catcccgtcg ccgttctccg gcacggttaa ggaagtcaag atcaaggtcg
     7741 gcgacaaggc ggctcaaggt acactggtcg cactgatcga agccgccgaa ggcgctgcag
     7801 cagtcgaagc accgaagcct gctgcagaat cggcgccggc cgcggcgcct gttgctgctg
     7861 ccgtcgcgga aattccgcca ccctcgcgcc cggtgcctga agtaccgaaa ccgatcgcgc
     7921 cacaaggcac gcccaacccg gtcgccgcgc acgccgaatt cgcccccggc aagaacccgc
     7981 atgccagtcc ctctgtgcgc aagttcgccc gcgaactggg cgccgacctg acccgcgtca
     8041 ccggcagcgg gaccaagggc cgtatcagca aggacgacgt gcaggcctgg gtcaagggcg
     8101 aactgaccaa gccgcgcgca gaagctgcgg caggcggcgg tctggcggta ctcgcattgc
     8161 cgtccatcga tttcagccag ttcggtccgg tcgaaacgca gacgttgccg cgcatcaaga
     8221 agatttccgg ggccaacctg catcgcaact gggttactgc gccgcatgtt acccagttcg
     8281 acaacgccga catcaccgat ctggaagact tccgcaagtc gatgcaggac gaagccaaca
     8341 agcgcggcgt caagctcacc acgctggcct tcctgatgaa ggccgtggtc aacgcgctgc
     8401 gcacttaccc caatttcaac gcatcgctgt ccccggccgg agatgagctg attctgaagc
     8461 agtactacaa cgtcggcttt gcctgcgata cgccggatgg cttggtcgtc cccgtgatcc
     8521 gcaacgtcaa cgagaaggac gtgctggaca tcgcgcgcga ccttggcgaa ctctccgcca
     8581 aggcgcgtga gcgcaagctg aaggtcgagg aaatgcaggg cggctgcttc accatttcca
     8641 gcctgggcgg cattggcggt acggcgttca cgcccatcat caactgcccg gaagtggcca
     8701 tcctcggcgt ctcccgttcc agcatcgagc cggtgtggga tgccaagagc aagacctttg
     8761 aaccgcgcct gatgctgccg ctgtcgctgt cctacgatca ccgcgtcatt gacggcgccg
     8821 acggtgcgcg ctttaccagc cacctgcgca tgatgctgtc tgacgtgcgc cgtctgctgc
     8881 tgtaattggg aattgtcatg agccaaacca ttgaaatcgc agtgccggat atcggcaatt
     8941 tcgacagcgt cgatgtcatc gaggtccacg tgaaggtcgg cgacagcatc aacgtcgacg
     9001 attcgctgat cacgctggaa tccgacaagg cgtcgatgga cattccgtcg acccatgcag
     9061 gcacggtcaa ggccgtgacg gtcaaggtcg gcgacaaagt cgccaagggt accgccatcc
     9121 tgacgctgga agcattggct ggcgaaacta ctccgactgc tcagccggca gcccctctcg
     9181 cagctgcact agcctcagct acagcacagg cgaccgcacc caccccggcc gccgccactg
     9241 caggcccatc cgacctgcac gccgaagtgc tggtgctcgg cgccggcccc ggtggctata
     9301 ccgctgcttt ccgcgctgcc gacctcggca agaaggtcgt attgatcgaa cgctatccat
     9361 cgctgggcgg cgtctgcctg aatgtgggct gcatcccttc aaaggcgctg ttgcacgcag
     9421 ccaaggtcat taccgaagct gaagagatgg tcgagcacgg catcagcttc ggcaagccaa
     9481 acatcgacat cgccaagctg aaggcctgga aggaaaaaga cgtggtgggc aagcttaccg
     9541 gcggcctggg agcgctcgcc aaacagcgca acgtgactgt ggtcaatggc cgtggcgagt
     9601 tctcctccac caaccagatc aaggtgacag ctagcgatgg caaggtgacc accatctcct
     9661 tcgactacgc catcatcgct gccggttcgc aggccacaaa aatgccgggc ctggcgccag
     9721 agcatcagaa tgacccgcgt attctggatt ccaccagtgc cctggcgctg gaagacattc
     9781 cgaagcgcct gctggtcgtc ggcggcggca tcatcggcct ggaaatgggc acggtgtacg
     9841 atgccttcgg cagcaaggtc agcgtagtcg aactctccga tggcctgatc cccggttgcg
     9901 accgcgactt ggtcaagccg ctgcacaagc gcatggaaag gcgcttcgag aaaatcctgc
     9961 tgaagaccaa ggtcgccaag ctggaaccga agaaggacgg cattcacgtc acgtttgaag
    10021 gcgacaatgc cgtagaacca caagtctacg accgcgtgct ggtcgccatc ggccgccgcc
    10081 ccaacggcag gaacatcggc gccgaaaagg ccggcgtgtt cgtcaacgaa tacggcttca
    10141 tcccggtcga caaacagcaa cgcaccaacg tgccgcacat cttcgccatc ggcgacatcg
    10201 tcggccagcc catgctggcg cacaaagcgg tgcacgaagg caaggttgcc gcggaagtga
    10261 tcgacggcca caaggtcgag ttccaggcac tggccatccc gtccgtcgcc tacaccgacc
    10321 cggaagtggc ctgggccggc atttccgaga ccgatgccaa ggccaagggc atcgaggtcg
    10381 aaaaagccag cttcccgtgg gtggccagcg gccgcgcact gtccatcggc cgtaccgaag
    10441 gcagcaccaa gctgatcatg gagaagggta gccgccgcat catcggcgcc ggcatcgtcg
    10501 gtcccaacgc cggtgaactg ctggctgaag ccgtgctggc gatcgagatg ggggcggatg
    10561 ccgaggatat cggcctcacc atccatgcgc acccgaccct gtcggaaacc gtgtgcttcg
    10621 ccgcagaaat ggcggaaggt accatcaccg acctactccc accacgcaag aaatagccgg
    10681 ccgggatgga cgtcaagctg cgtccatccc acctccccgc cgctacgcta tcgaagcaag
    10741 gctaatagct cagcggggga atctatcatg gcagatgcac cccattcctg cggcttatcg
    10801 tttgcgtcca gataaccata acaggctacg atagttctca tccctgcggt attgcccgca
    10861 atgatatcgc gctcggcatc ccccacgtag caacaggctt ctggatggat tttcgccaga
    10921 tcacaggcga gcaacagggt atctgggtgg ggcttgggac gtgtggcatc gtctccgctg
    10981 acgatgcagg cagcccgctc tgtcagcccc aatgccagca tcaagggctg agtgaaacgg
    11041 cgcggtttgt tagtgaccac cccccaagcc atcccctcgt tttccatcgt ctccagtatc
    11101 tccgcaatgc cgttgaacat gactggagag cgggtgaaca cctgatcgta cagatccagg
    11161 tattcctcgc gcatggtgga aaaatgagca tgcccggggg ccagtccgaa accaacgccg
    11221 agcagtccac gcgaaccgtg ggaggcatag ggacgtatgg tttccagcgg caacggaggt
    11281 aagccgtgac gctcacgctg catattgagt gcaagagcca aatctggggc agtgtcgacc
    11341 agcgtaccgt caaggtcgaa tagaacagtc ctggagttca agatttgatg gtgtgaatga
    11401 tgtagttgac ggaaacatcc tcgcccagtg tgtaacgctt gctgaacagg ttgtagttca
    11461 tgccggtgat accggacacc tcaaggccgg ctgtacgaca ccaggcggac agttcggaag
    11521 gcttgatgaa tttctggtat tcatgtgtgc cttttggcag caggttgagc acgtattcgg
    11581 ccccgatcac ggcgagcagg taggacttga ggttacgatt gagggtggaa aagaacaccc
    11641 ttccacccgg ttttaccagc cgggcgcagg cgcgcaccac tgtagcagga tcgggcacgt
    11701 gctccagcat ttccatgcaa gtcaccacat cgtagcttgc tggcttttcg tcagccaaag
    11761 tctcaactgc caccaggcgg taattcacca ttgcgccact ttccagcttg tgcaactgcg
    11821 ctaccttgag cgccttttcg cccaggtcta tgcctgtcac ctcggcaccg cgttgcgcca
    11881 tcgattcaga caggatgccg ccgccacagc caacatccag aacgcgcttg ccagccaggc
    11941 cggcaacgtg gtcaatgtaa tccagtcgca gtgggttgat gtcgtgcaac ggcttgaatt
    12001 cgctggtggg gtcccaccag cgatgcgcca gcgcagcgaa tttctgcaac tcggccggat
    12061 cggcgttcaa tggtgaaact gtttcagcct ggcttgccat ctggtagtga tctcctgtaa
    12121 ttctgcaatg tcgacactgt catggacgcc gcgctggtag cgtagttccc cagccaccca
    12181 agtatggcta acctgctcgc gcccagcgac gtaaatcagg tgagacaggg gatcgaagca
    12241 cggctgcatt tccgggctgg agaagtctac cgccgccaga tccgccagtt ttccgggctc
    12301 gatggagccg atatggtcat ccagtcccaa cgcgcgtgcg ccgttgagcg tagccatctc
    12361 caatgcctgc cctgccttca gagtggaggc atcccctgcg gccttggcca gtagtgcggc
    12421 cagccgcatt tcagaaaaca tgtccagtcg attgttgctg gcagcactat ccgtacctat
    12481 tccgatattg attccggcag ccagaagtgt cgatattggc gcgatgccac tggccagctt
    12541 gaggttggag ctgggacaat gcgcaacatg gcagccctgg gctgacagag tttctatttc
    12601 gcccagcgtc aggtgtacac aatgcgtcac cagcagattc gggccaagaa gacccagccc
    12661 ggtaagacgt tgcaacggcc gcaatccata ttgcgcctcg ctctgtgaaa tttcttctcg
    12721 cgtctcatgc aggtgggtat gcaggttgag cccaagctgg tctgcatagg tcagcagctt
    12781 gccaaaactg cggtcgctga cggtataagg cgcatgcggg gccaggcacg tggtgatacg
    12841 ctcgcgacca cgcaaagcat cgcgcgccgc caatccctta ctcagataat catcggcatc
    12901 ggctgcataa cctgagggaa attccagcaa caccagcccg agacaggcac ggataccgga
    12961 acgctccact gcctcggcag cagcctgagg gaagaaatac atatcattga agcaggtcac
    13021 gccaccgcgc agcatttcgg cgcaagccag caaagtacca tcgtgcacga agcgcgcgga
    13081 taccagttcc acctctgccg gccagatatg tgcatcgagc caggtcatca atggcacgtc
    13141 atcagccaat ccacgcatca atgacatggc ggcatgcgta tgcaggttaa tcaagccggg
    13201 aatcaggaca tgtgtcggta aactgaccgt ctccaacgca tgaaagcact ggcgcgcctc
    13261 ggcaatcggc aatatggcca tgacccgccc ctggtcaatc gcaatcgcgg aattttccag
    13321 taccacccct cgcggcatca cagggataat ccagcgtgct tcgataagca ggtcaactgt
    13381 gttgatatgg gcttccggca tagacaacct cttaaaaaaa aagccccgac atgcggggct
    13441 tttttaattt cagatgaagt tattactgtt gtggttcaac aaccacaccc tggacttcag
    13501 tttgctcaac tgtaacttct tcgactgcac gctggcgttc gacttcaacc accacgcgac
    13561 ggttaggttg caggcactcg atcagcttct tgccgcgaac acccttgcac tcggcgatgg
    13621 gatcggcctc acccttacca actgcatgca gacggttttc ttcgatgcct tggctgacca
    13681 gatacttctt gacggcgttg gcacggcgct cggacagctt ttggttgtac ttggcatcgc
    13741 cgatacgatc ggtatatccg gtcaccagca ccagctcggc ttcagggtgc gccttcattt
    13801 tctcgaccac ttcggtgttg agcagtgact taccttcgtc cttcagggta gcattgtcga
    13861 aaccgaacag cacttcggct gccagcgtta ccttcacagc aggcttggcc ggaccctcct
    13921 cgacgacagt ttcttcaacc gtgaattcat acacttcatc ctcaaccgga gcaacttctg
    13981 gcaggccggc agcgacaggg gcagcaacga ctacagcctt cgggggcggc gggaagttga
    14041 tgataagacc aaggttggta taaaggttgc ctacagtctt gctatcggta ttgtttgccc
    14101 cagtatagga ctgcggcaca tcggcccggc tccatacctc acgcaagccg aactgcaaac
    14161 cgatgcggtc ggtgaaggaa tactggaaac cgaaccccag cttgccgtac acggagttgt
    14221 tggagtcacc gtaattcgta ccattggcat tatagtcgac gtcgttgtgt gcgtaaccca
    14281 agccagccag caagtaaggg cggaactttt cgcggctgaa gagatacaac gcatctacgc
    14341 caaacagggt ctgcttgtag tcaccaccca cgccgctctt gtcggaatct gccttggcat
    14401 aagtgccccc gatttgcacg tcccagtgct cgctaagctg cttgcccaag gaaatgccat
    14461 aagctggcgc ccagctttcc gcatccaggt cgctatcggc atgcatcatg ccgatgttcg
    14521 gcatgacgta ccaggaaccc tcgaattcat cagcagcaaa ggcagcgcca gcagacattg
    14581 caaacgcgcc ggccacagcc agaccaatca acttgttttt cattttgtga aacccctttg
    14641 tttagaactc tcgcgaacta tactaaatgc ggtatttacc ggcaagccat ttattgaatt
    14701 aatgcgcatc aaccgccaat ttgttgcatt cacgcaacat cgattgcaca gactcgaggt
    14761 gtgtgcgatc gagcatcgct ttgcgctcat ggtttgcaca cagggcgcct ctaaaaccca
    14821 gataatctgg tttcaacaaa gacaaagacg caatgtcgga ttccataaga gaaccggcca
    14881 gccctacttg catacgtttc gatctggagg cgatgacgaa atcgtgcaag gcatcaaggc
    14941 caagatagtc cgtcaagcgt cctccgttct tgttttcggt atccagcatc acaccataga
    15001 agccggcatt ttccagcaac ggcagcaact tcatgtccgg tcgatgatcc gcaaacaaca
    15061 cggcgacaat gcacgccccc atagccacga gcggcgtcaa cgcccgcacc gcctcctgat
    15121 gcccggtatt tccaaagaaa ccgaccttga tgatatccac gccggtgctg gccgtttcag
    15181 ccacccgccc agcgagaaga cttggctgca cgggcaaatc gcctatagtg gcactgaccg
    15241 gtcgccggcc gtccacgacc ttgacgattg ccctgatgac gtccagcggc aaggctccta
    15301 gcgtgccctg cgccgggttt ttcaggtcaa tgatgtccgc gcctgcctcc agcgcgatca
    15361 tcgcctcctc tgcactggta acactggcaa gcagccgtgt cacgccaggt tctccccgcg
    15421 catgccggcc ttgtagttgt cgattttttc catcagccag ctccatgcct ccaactcacg
    15481 ctcgccagca gtcttttcga cggcgatcgt cagatagttc acctcgtgct caatcttctc
    15541 aagcggcagc agatgcagcc gacttaccag caccgctgct tccagcacgg ccgcctgggc
    15601 acgattgaag ccgcggaaag gggcatgaat cgcctcatgc accacacgaa acagcagttc
    15661 cggacgcacc gcatcctcct gtacatcgac cagctccagt tcgcggtgcg tcaaggcagc
    15721 gtccagcacc cagccatcaa ttttgttcgc ccgtcgcaca ggccaatcgc gccgtccagt
    15781 cagcgcacca gcaaaaatac gcacatcaac ggtggcgttg accaccgcgc agcgcccatg
    15841 cagcaagtta tccaggctgg atgaaggtcg aaatggggct atcagcacca gaccatcgcg
    15901 ctccctcacg ccgaacggcg cgatgtgcac tttgccatct gcatcactcg aggtaatgat
    15961 ggtttcgtta atcatttgcc actcatgtcg cgctttttct tgagcgaagc ttcccggtgg
    16021 gccacacgac cacgcgcgtc gggctcggcg gaattgcagc cccactgcaa ttccgcatcc
    16081 tggctgtagc gcttcttcag ttgccaggcg acttgtgctc gcgccagttc aacaccaaga
    16141 taaaaagcat gcgaggcatc atcgtccact ttcaagtatg gataaagtcg gaacgggtcg
    16201 atatcgcaca acaggccgtc gcggttgtag atatggacgc cctgctcgct gacctggata
    16261 cgaaagttgg gatccttcac ctgcgctgcc agttcggcaa tctcagcctc gctgtaggga
    16321 aacggccggc ggtcgtgcaa gccgagcaag tcattggaat aatcacgtgg caggcgacga
    16381 tcctcgtgtg cggcatgcat cacgcgccgt gccacatcgg cttcgcgtac cgcgttgcac
    16441 gcgtgcgggc tgaccgaggt cgtcagcacc gcgttcaagt tcagttccgc gataacgccg
    16501 aacagcacag cattgatgcc ggtagtgtca gcgtcggtca gttcagtcag gttgcccacc
    16561 cccatcatga tctggatgtc cgggtgtttt ttgcgcagcc tgtgatagcg aactatggac
    16621 tccgccagac caaacgggat ggggtcgaga atggggtcgg cgatgaacgc ctggcccttc
    16681 ttttgcatcg cctcgatggc gcggtagagg gaaggcaggc tgcccggcct ggcgggaacc
    16741 agcaccggca tagccgtcac ctcgtcggcg atccacagcg ttttctcagt caggctcagc
    16801 aggtagtcgg caccagcccg gccgccacgc agaagctctt cctgctgcat cgaatcgata
    16861 ctgacctgca taccagccgc cttgagcgcc tgcacggcgt cttccagatg tggaaacggt
    16921 gtatttggca gacagccaag gtcgatcaca ttggcaccct gcgcgcggta gcgctcggcc
    16981 cgggcaagaa tatctgccac cggcaattgt ggtgcctcga cgatttcggc aaaaattttc
    17041 acatcgtagt tggacaaatc gggttctttg ccgccatgtc cgaaatactg cggaatgtcc
    17101 ttgaggtctt ccggccccag ctgtaccggc acaccgtatt tctcttgcaa cggcaccaga
    17161 ttgccgcggc ataggcccgg cagcacgatg cggcctgctc cgcgtgcgtc aggcagacga
    17221 cgagcaataa attccggggt catgagcgct gcgacgctga taccgagctg cgccacctga
    17281 taatcgaatt gtgccggttg cattccggcc agcaccttgt gcaggctgct ttcggccagc
    17341 ttgccggtca gaaagaggat gttttcagac acgccagacg agcctgtatt gccgcgtcca
    17401 gttccaccat gtcggcggcg acggtagttt cctcaaaagt tttgagcttt tccatgtttt
    17461 cgaggtcgat gcggcgcgga tagaccttga ccatgtgctc ccgcggcgcc tccgattcca
    17521 attccggctc ggtatcgcag gcaaacacga tggtcggcac ccgacccttg ccggcttgag
    17581 cgaaaatgtt ggtcactagg ttatcggaaa tgccgcacac catcttggcc acggtgttgg
    17641 aactggtcgg cgcgatgacg accgtgtgat aaacaccgtg atagaacagc tccaccggca
    17701 ccgccgaagc ggtcttgtcc tgaaacacgc gtccaatgct gtgggcgtac ccatactgct
    17761 gcagaatttc cgccgctgcc ttgctcagga acaggtcaac atcctgcaac ttgccgatga
    17821 tgtccaggca ttcgcgcaaa taatggccag agccagtgat cgcccaagcc aggcgctgct
    17881 tttcctgttt cagcgccaac tcaggctccg aagggatggc gcagcacgat ggtttcgtcg
    17941 cggtccggcc cggtggagat gatgtccacc ggtactccgc agacttcctc catgcgcctc
    18001 aggtagcgct tggcattttc cggcaggtct tcatagcgct tgatgccgaa ggtactttcg
    18061 ctccagcctg gcagttcctc gtagatcggc tcgcaatgct ccagcgcttc ggcgccgacc
    18121 ggaaaaatgt cgtgcgtgac gccgccaatc ttgtagccca cgccgatctg caccgtttcc
    18181 atgccatcca ttacgtccag tttggtgatg cacagtccgg acacgccgtt gacttggatc
    18241 gaacgcttga gcgcggcggc gtcgaaccag ccgcaacggc gcgcgcggcc agtggtcgag
    18301 ccgaactcgt tgccacgctt ggccagctgc tggccaatcg gatcaagctt gtccaccgca
    18361 tcgtacagct cggtgggaaa cgggccggag ccgacgcgcg tggtgtaggc cttggtgatg
    18421 ccgagcacgt agctcagcat ctgcggcgcc acaccgctgc cggtggtggc gccaccggcg
    18481 atggtgttgg atgaggtcac gaacggatag gtgccgtgat ccacatccag cagtgcgccc
    18541 tgcgcgccct cgaacagcag cggctggccg gcctgattgg ccaggtacag caggttggaa
    18601 acatccgcca ccatgggctt gatctgctct gccagcgcca aggtgtcatc cagcgtgcgc
    18661 tggaagtcca cggtttcggc atgaaaatag ttcttcagca cgaagttgtg gtaatccagc
    18721 acttcgccca gcttggcggc aaaacgctcg cgatagaaca ggtcctgcaa cctgatcgca
    18781 cgtcttgcca ccttgtcctc atatgccggg ccgatgccgc ggccagtggt gccaatcttg
    18841 gccgccccct tggccgcttc gcgcgcctgg tccagcgcca cgtgataggg caggatcagc
    18901 gggcaggcct cgctgatcct gagccggctg cgcacattga cgccagcttc ttcgagcatg
    18961 cgcagttcgg tcagcagcgc ctgcggcgac agcaccacgc cgttgccgat gaagcactgt
    19021 acggattcgc gcaaaatacc ggacggaatc aagtgcagca cggttttctt attgccgatc
    19081 accagcgtgt ggccggcatt gtgcccgccc tggaagcgaa ccaccccgtg ggcgtggtcg
    19141 gtgagccagt ccacgatctt gcctttgcct tcatcccccc actgggtgcc gattacgacg
    19201 acattcttat tcatgcgatt tctacctgga ttcaaattct gcaacgggaa ccactttcca
    19261 ggttccctgt ttattggtaa gtgcgcggtc gcacccgagt tcattgcggt acgcctcgtg
    19321 accgggcagt tcaaccacca cgatttcacc agccgcacgc agcgcagcga ttgcttcctg
    19381 caatccggca tcttcgcgga aaggcgccag tatcgccgct tgcgacgggg gcggaggtag
    19441 agcggtggtc aggccacgca aatcaagact gaagccggtc gcagggcggg ctcggccaaa
    19501 tattgcgcca acctcgtcat aacggccgcc ctgcgccaac ggtccggaat ggccttgggc
    19561 gtaggcggca aacaccagcc cactgtgata gtgatagccg cgcaactccg ccagatcgaa
    19621 ggaaatctct acatcgagtc ttgacagctg ctgcgagatg cgccgcgaat cctgcagcgc
    19681 ctgcttgatt tcggcatatt gcggcagact tttctccgcg gccagcagca cttcggcgcc
    19741 accattcagc tcggtcagcg ccagcaacgc ctgcctgatt tcggcatcga ggccggcggt
    19801 cagggcattc aactcggcct tgcccttgct ctgcaacgcc atcaacaaat cctgttccag
    19861 ggctgtggca ataccgctac gttttacaag gctgcggaaa attccgacgt ggctgaagtc
    19921 aagctgcagc gcctgcaccc cgaccgcctg caatgcttcc actagcaact gctgtatctc
    19981 aatatcgctc tcgatgccgg catggccgaa caactccgcg ccgatttgta gcggctgtct
    20041 ggtctgtgcc aacccgtccg gccgggtgcg cagcacgctg ccggcatagc acaggcgcgt
    20101 caccccctgc cagttgagca ggtgggcatc gatgcgcgcg gcctgcggcg tgatgtcggc
    20161 gcgtatgccc atcaggcggc cactcaataa atcgaccacc ttgaacgtcg ccaggtcgag
    20221 atcatgcccg gcacccgtgg tcaaagactc caggtattcc agcaaaggcg ggatgaccag
    20281 ttggtaacca tgaacgcgga acaggtccag caggcgccgt cgcacctgtt ccatgcgcgc
    20341 cgcctcggcg ggcagaacat cttcgatata ttcaggaaga agccagttgc gcatatgctt
    20401 aacgggtaac cagcagcagc aacaagccgc ccgccatgga gagtaccccg acaaagcgca
    20461 actgaccgtc gctgaactcg gtcatgcgcc tgaacgtctg gcgccaggcc tgcggtgcca
    20521 gcaatggcaa tattccctcc agcaccagca tcagcgcaaa agccgcgatc aaggagttat
    20581 ccagcacgtc ttacttcctt ctgccgtcag catcacgcat gtatttgagg aagtcggagt
    20641 ccggctgcaa caccagcacg tcgctcttgt tcgtaaagct gttgcgatac gcttccaggc
    20701 tgcggtagaa cgcgtagaat tccggattcc tgccataagc ggaagcatag atttctgctg
    20761 ccttggcatc gccctcgccc ttggttttct gcgcctcgcg ataagcttcg gcgatgatga
    20821 cctctcgctg cttgtcggcg tcggcacgaa ttttctcggc agcgcctgca ccctgggagc
    20881 gcaactcgtt agcgacgcgc ttgcgctccg cttccatgcg ctgatacacc gagccactca
    20941 cctcctcagg caggtccacc cgcttaattc gcacgtcgag cacttcgata cccatctgac
    21001 gcgcttcacg atctgccttc tggcgcagga tttccatgat ctggtcacgc tcgcctgaca
    21061 ccacgtcatg aatggtgcgc ttgccgaact cggcacgcag accatcattg acggtctgcg
    21121 acaaccgcgc ctctgcctgg cgctcgtcgc cacgcaccga aacgtagtac ttggcggggt
    21181 cggtaatgcg ccatttgacg aaggaatcca ccagcacatt tttcttttcg ctggtgataa
    21241 accgatccgg ctccacccag tccagagtga cgatgcgttt ttcaaaataa cgcacgtttt
    21301 ccattagcgg aatcttgaaa tagaggccgg gctccttttt aactgcaacg atctcgccca
    21361 aacggaatac cagtgcgaac tcacgctggt cgacggtgta gaccgacatg ctcagcaaca
    21421 ccaatgcgat gagcaggccg gccaacaggc tacctagatt tttcatggcc gcccccccct
    21481 ttcgcgactt cggaatgctt cgcgtgaacg ttgtgcaccg ccatccagga cctctggtcc
    21541 cgttgcagca ggccccgtgg ccggaggtgc ggaaggtgcc gccgtgccag tgttggccag
    21601 tagcttgtcc agcggcagat acaacaggct gttattgcct ttctgatcca ctaccacctt
    21661 gctgacgttg gaaagaattt gctcctgcgc atccagatac agacgctgac gcgtcacctg
    21721 cggcgcctgt tcgtactgcg aaagaatctg gctgaaacgg ctggcgttac cctgcgactc
    21781 gttgaccacg cgcagcttgt agccggcggc ttcttcgagc aagcgtgccg cagtaccgcg
    21841 cgccttaggg atgacatcgt tggcgtaggc ctgaccttca ttcttctgcc gctccagatc
    21901 ctggccggct ttgacggcat cgtcgaacgc cgcctgcacc tgctccggtg gttgcgcgtt
    21961 ctgcatggtg acattgacga tgttgatgcc ggattggtag cggccgagga tatcctgcat
    22021 cagcttctta acacgcgcag caacctctgc acgcccctca taaagtacga aatccatctt
    22081 gcttttgccg accacttcgc gaatggcggt ttccgccgcg ccgcgcacga cactagcctc
    22141 aggattaccg taggactggt ctgaaaaacg gttgttgaac aaatagtcgt tggcactctt
    22201 gaggttgtac tgaatggcaa
//
LOCUS       DAOMFA010000006        21505 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_138_length_21505_cov_4.773427, whole genome shotgun sequence.
ACCESSION   DAOMFA010000006 DAOMFA010000000
VERSION     DAOMFA010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 21505)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21505)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21505
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_138_length_21505_cov_4.773427"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(47..373)
                     /locus_tag="PKW44_00680"
     CDS             complement(47..373)
                     /locus_tag="PKW44_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2934 domain-containing protein"
                     /protein_id="HQR50135.1"
                     /translation="MAEAKTKPAAQKAAPAAKPAAAKKSSVAAPKTAAAKKPAAPKKA
                     AAPKAAAAKKPAAAKKVVAARGAEERYRMVEVAAYYIAERNGFAGDPTVFWTEAEAQI
                     TKLLSK"
     gene            complement(461..1936)
                     /gene="tldD"
                     /locus_tag="PKW44_00685"
     CDS             complement(461..1936)
                     /gene="tldD"
                     /locus_tag="PKW44_00685"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HQR50136.1"
                     /translation="MKPETLPSASSPEPGTLFTTAADTLLVPYQLDHHALQGVLGSIM
                     SHKVDYADLYFQYSRAESWGLEEGQVKSGSFSIDQGVGVRAVSGEKTAFAYSDDINLP
                     ALQQAALATRAIAAQGSERSAQAMQRSDTPRLYLPHDPIASMSDDSKVRLLERLEKYA
                     RELDPRVTQVMASLAGEYEVVLIARHDGMMAADIRPLVRVSIQVIAEADGKREQGSSG
                     GGGRFDYGYFSDDVLHGYAKQAVHQAITNLDARPAPAGAMTVVLGSGWPGILLHEAIG
                     HGLEGDFNRKGSSAFSGRIGQKVASDGVTVVDDGTITDRRGSLNIDDEGTPTQRTVLI
                     ENGVLKGYLQDSLNARLMGAALTGNARRESYAHLPMPRMTNTVMLNGDKSPEEIIRSV
                     KKGLYAVNFGGGQVDITSGKFVFSAAEAWMIEDGKLTYPVKGATLIGNGPDVLTRVSM
                     IGNDMALDSGVGTCGKEGQSVPVGVGQPTLRIDGLTVGGTV"
     gene            2035..2979
                     /gene="miaA"
                     /locus_tag="PKW44_00690"
     CDS             2035..2979
                     /gene="miaA"
                     /locus_tag="PKW44_00690"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HQR50137.1"
                     /translation="MTDSRLPPAIFLMGPTASGKTGLAVDLAQRLPVELISVDSALVY
                     RGMDIGTAKPDAATLARAPHHLIDIIAPTASYSAAQFRDDALRLMGEITQRGNIPLLV
                     GGTMLYFNTLQHGLNNLPQADPVLRAEIDARAEREGWPALHAELAQVDPATAARLMPN
                     DAQRIERALEIYHLTGRPMSELLREEERASLPYRLLKIALLPSDRAVLHQRIAERFDA
                     MLQAGLLDEVARLRREVPALSLNLPSMRCVGYRQAWQHQDGEFDLATLRDKGIAATRQ
                     LAKRQMTWLRGMDDVEELDCLESGLAEELEQRVREFIG"
     gene            complement(3128..3589)
                     /locus_tag="PKW44_00695"
     CDS             complement(3128..3589)
                     /locus_tag="PKW44_00695"
                     /inference="COORDINATES: protein motif:HMM:NF012534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HQR50138.1"
                     /translation="MNGKLTKWNDERGFGFITPTQGGQEIFVHISVFPKDGQRPHIGE
                     SLSFEIEIDNDGKKRAKSVSRPLRANASYRDQPVPTRHHEKHGLRGLIVPLFILGAVG
                     VYGYGSHMHKFSEPAADYSTESAEPPDATDLHPPQNSQLIIAVMVVPIARK"
     gene            complement(3609..4796)
                     /gene="dapC"
                     /locus_tag="PKW44_00700"
     CDS             complement(3609..4796)
                     /gene="dapC"
                     /locus_tag="PKW44_00700"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009016 - succinyldiaminopimelate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019877 - diaminopimelate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyldiaminopimelate transaminase"
                     /protein_id="HQR50139.1"
                     /translation="MNPNLDKLHPYPFQKLRDLFAGITPNAALKPVNLSIGEPKHATP
                     QFIKDVLTANLGGLASYPTTLGSVELRQSIADWLVRRYGIPTPDIEKQIVPVNGSREA
                     LFAFAQAVIDPGKNKPVVLSPNPFYQIYEGAALLAGAEPWYLNTLPEHGYRMDFDAVP
                     EAVWQRTQLMYVCSPGNPTGKVYNLDDWREIFTLADKYGFVIASDECYSEIYFEEGHA
                     PLGALQAAHQLGRNWERIVMFSSLSKRSNVPGMRSGFVAGDAAILEKFLLYRTYHGCA
                     MSPAVQAASAAAWNDEAHVVENRRLYTEKFRTVTPMLKNVLDVELPDASFYLWARVKG
                     SDAEFARRLYRDCNVTVLPGSFLAREAHGVNPGAGFVRIALVASLQECVDAAKRMQKT
                     SFS"
     gene            complement(4932..5540)
                     /gene="thrH"
                     /locus_tag="PKW44_00705"
     CDS             complement(4932..5540)
                     /gene="thrH"
                     /locus_tag="PKW44_00705"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoserine
                     phosphatase/homoserine phosphotransferase ThrH"
                     /protein_id="HQR50140.1"
                     /translation="MQLVCLDLEGVLVPEIWIEFSKRTGIPELSRTTRDEPDYDKLMT
                     YRLDLLKQHKLGLADIQKVIAEMGPMQGAREFLDALRRDFQVVILSDTFYEFAMPLMA
                     QLGMPALFCHKLETDGAGFLANYHLRMPNQKKEAVQRFKELNFKVIAAGDSYNDTAML
                     GEAHAGILFHPPQNVIDEFPQFPVTNSYAELRAAIDAASARI"
     gene            5667..6545
                     /locus_tag="PKW44_00710"
     CDS             5667..6545
                     /locus_tag="PKW44_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HQR50141.1"
                     /translation="MSKPDRSIPTFGLLFGATVWGIIWYPYRLLEQAGISGVASSLYT
                     YSIALMLGALLFALRRRGLTVVPASIFWLALAAGWTNLSYVLAVIDGEVMRVMLLFYL
                     SPFWTLLLARLMLDERVGLVGTAVVVLSLCGAALMLWQPESGLPLPQNTAEWLGLSSG
                     MGFALTNVLTRKSTHLTLQAKSLAVWVGVVAMALLFLPFESRPLPLPGEVGLSGLLLM
                     GGIALLLMLATLLVQYGVTHMPVTRASVIFLFELVVAAVTSYFLAHEVLSVREWVGGA
                     MIVAAALFAARMEEHA"
     gene            complement(6542..7630)
                     /locus_tag="PKW44_00715"
     CDS             complement(6542..7630)
                     /locus_tag="PKW44_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HQR50142.1"
                     /translation="MAALDISPVLKFMVEKNASDLFFSTGAPINIEIEGIAMPINSQI
                     MPPGMVKEIAYSLMNEALIREFEETLEVNFAHSVSDLGRFRINAFLQRGEVSMVVRHI
                     KSAIPSLEELGLPMLLKEVSVRPRGLVLVVGSTSSGKSTTLAAMINYRNENKTGHILT
                     LEDPIEFIHNHKKSVVDQREVGIDTKSFENGLRNAMRQSPDAILIGEIRDMDTMRHAI
                     AYAETGHLCLATLHANNATQAIERILTFCPPDMKQSILLSLALNLAGIMSQRLIPGTQ
                     AKRVLAMEVMLNNHYVSDMLMKGKLEELKQVLSSSNDIGMCTFDQSLFQLFQEGKIDE
                     ENAVYYADSRNDVSLKIRLAREGARTAD"
     gene            complement(7676..9523)
                     /locus_tag="PKW44_00720"
     CDS             complement(7676..9523)
                     /locus_tag="PKW44_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020181971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQR50143.1"
                     /translation="MQDRQIEQLESAIRRAPQDPELHYDLGNLLLGQRRPAEAESSFL
                     AALRLAPGHPQILLQLGNARSAQGRYADAVSQFQAALRADPAQPAAHYNLGNALREMG
                     QPEQAAASYRAALKLDPRDADSHNNLGNVLREMGKLDEAIARYREALRLNPGLHHARM
                     HLLHQRQHVCDWAGMDDEIAEIRRLVREEPRAQISPFAFLALPGTTAAEQRLCAEHWA
                     ENRLGQLVEDGKRLAYPHTRENKFKLKIGYLSADFRQHPLAWLATELMELHDRESFEV
                     HGYSYGPDDHSPARRRWEQAFDHFRDIRDLSMHEAARKIHADGIDILVDLTGFTHSSR
                     SGILALRPAPLQVNWLGFPGSMGAPFVDYLISDSFITPPEQAQHYAEQLALLPDTYQP
                     NDRQRPLAATPSRAECGLPEHGFVFCCFNQTFKITPQLSGLWLGLLKSVPGSVLWLLE
                     CNPWAKANLRREIAARGVEPGRLIFAPRVPIEQHLARHRCADLFLDTLPYNAHTTTSD
                     ALWMGLPLVTCAGETFAARVAGSLLRAAGFPQLVTGNLHDYQALALRLATSPDELHAL
                     RTVLAQQCDTAPLFDTPRFARHLQQAYRAMWQRYASGQPPQAINLAEPQ"
     gene            complement(9534..11474)
                     /gene="mnmC"
                     /locus_tag="PKW44_00725"
     CDS             complement(9534..11474)
                     /gene="mnmC"
                     /locus_tag="PKW44_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020181970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)-methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016645 - oxidoreductase activity, acting
                     on the CH-NH group of donors [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HQR50144.1"
                     /translation="MTSTQTKLEWRDGQPISAIYGDVYFSRESGIAETRHVFLQHNRL
                     AGRWHGLRQPLFTIAETGFGTGLNFLCAWQLWRECAPSDARLHFVSTEKFPLSSDDLA
                     KALTLWPELSGLSAQLLEQYRWLAPGWRRMEFDDGRVTLTLLIGDARATLPQLRASVD
                     AWFLDGFAPAKNPEMWQPELFAQIARLSVPGCTFATFTSAGAVRRGLEASGFNVEKVA
                     GFGTKREMLCGEYAGGTTSQPALPDKHAMVIGGGIAGAASAWSLASRGWQVTLIERHG
                     ELAQEASGNPQGVLYPRLSGHDIPLSRVAQTGFMTTLRLLQRLLPKGQDWDDCGLLQQ
                     AFNAREAKRCEEVLARGLPPDLVRAVDVGEAAALTGIAMPHGGLWFPGGGWVHPPALC
                     RALAAHPNITLKTSAQALQLLHGDHGWQVLDADDLVAEAPVVVVCSANDSTAFPQTAH
                     LPLEPVRGQITQLPATPASAKLSAVVCTEGYISPARQGTHCAGATFAPNEHSLELRAD
                     DHAENLAMLKHLSPALHQALGGSALDAARLHGRAALRCVAPDYLPLAGPLLDAGPVAE
                     RYSLGSRLPVEHLPWLDSLYVNTGHGSKGLLTAPLCAELIAAMLENAPLPVDATLARA
                     LDPNRFLLRQRGLKRLIGAAIG"
     gene            11499..12017
                     /locus_tag="PKW44_00730"
     CDS             11499..12017
                     /locus_tag="PKW44_00730"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQR50145.1"
                     /translation="MTHSSDACPSSWVTRFAPLIRQGGSVLDLACGGGRNARWLAQQG
                     WRVEAVDRDPAAIAGLRGVKNISALQADLEVAPWPYPGRKFDGVVVCRYLYRPLLPVL
                     AASLAEGGVLIYETFMLGQERYGRPSNPDFLLRPDELLEACSGALQVIAFEQGIFNET
                     NPAMLQRICARR"
     gene            12042..13841
                     /locus_tag="PKW44_00735"
     CDS             12042..13841
                     /locus_tag="PKW44_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HQR50146.1"
                     /translation="MFTKDIAMYRRLFITLSAAALLASCAHQPASHDAAEWPSYGRDY
                     SNQRFSRLDQVNRDNVTRLAPAWTYQSGVKATFQTTPIVVEGVMYVSLPYNHVVALDA
                     RTGQQLWKYEHQRRKDWKMCCGPANRGVAVSGGKVYIGTVDARLVALDARTGKVVWDI
                     DAVSENVVTEGIDALHADDPLSKERVSGGTGIGIAMAPVVYQGRVIVGITGVGYGLHI
                     EENRPDAPLGAVIGVAGRYGRPGFLAAFDAQTGKRVWQFDTIPPQGWEGKFSPTTPDG
                     VPLNRNIEQERATFTQYQEAWRYGGGSAWTTPAIDPELGLLYFGTGNPSPQMDDVSRP
                     GDNLYTVSLVALEAATGKLRWTYQQVPHDVWGYDLASPPILFDYQHDGNSIPAVGQAS
                     KTGWFYVHDRATGELLLKSEAFVPQSNLFARATPEGTRIHPGVFGGVNWSPAAVDEAR
                     QQVFVAAMHMPVRYSLHETPATDGKPAIRYTASEPADEPKWGLLSSIDLKTGRIRWQH
                     KTPEPLVGGVLATAGGVLFTGEGNGSFDAFNSDSGDLLWQYKAEAGVNAPPITYELDG
                     VQYVAVAAGGNQIWGYKQGDTLLVFKLDNSSGR"
     gene            complement(13984..14068)
                     /locus_tag="PKW44_00740"
     tRNA            complement(13984..14068)
                     /locus_tag="PKW44_00740"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14032..14034),aa:Leu,seq:cag)
     gene            14105..16327
                     /gene="rnr"
                     /locus_tag="PKW44_00745"
     CDS             14105..16327
                     /gene="rnr"
                     /locus_tag="PKW44_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020181796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HQR50147.1"
                     /translation="MSTRKKQRGKDPQAEREAANYAHPLPSREFILQVMADEGAPVSV
                     ERLYELLDIRKGERDIFNRRLGAMERDGQIFRNRKNALCIAQKINLIAGKVQGHPDGF
                     GFLIPDAGGEDLFLSPKEMAQVLHGDRVMARQSGLDRRGRPEGKIVEVLERANKRLVG
                     RLIREAGVVLVAAEDRRINQDILVPPGKDMKAQAGQIVMVELIEQPSAYAQPIGRVVE
                     ILGNYADPGMEIEIALRKHQLPYEFSAAAVQQAEQYPSKVQKKDWAGRIDLRELPLVT
                     IDGETARDFDDAVYCEPEGKGWRLVVAIADVSFYVKPGDALDKDAFDRGNSVYFPRRV
                     IPMLPEALSNGLCSLNPDVERMCMVCDMQVDGKGVIKRYKFYPSVMRSKARMTYTKVY
                     DMIVDPAGKTAREYAWLLPHVHNLYALYQALAKSREQRGAIEFETIETLMMFNDQGKI
                     DRIVPSTRNDAHRLIEECMLAANVCASDFLKEHHHPALYRIHEGPTLEKLEKLRGFMA
                     EFGFVLGGGDAPHAKDYGKLLARIKGRPDEQLLQTVLLRSMQQAVYSPDNVGHFGLAY
                     EHYTHFTSPIRRYPDLLIHRAIKAVLEGKKYKAGDWSNLGMHCSLTERRADDATRDVE
                     TWLKCFYMQDKVGETFNGTVAGVTGFGLFVALDEVYVEGLLHVTELGNDYFHFDNMRH
                     EMIGERTGTRFRLGDRLRVKVARVDMETSKIDFVLAQEDEAEQKTAPKNGGKKGGKKK
                     "
     gene            16324..17070
                     /gene="rlmB"
                     /locus_tag="PKW44_00750"
     CDS             16324..17070
                     /gene="rlmB"
                     /locus_tag="PKW44_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HQR50148.1"
                     /translation="MSVSRIVFGFHAVLSRIRQNPASVQEICVDKTRKDARMQDLLQL
                     AEQKGVRVMQMEKERLDGLAANGRHQGVLARVADTPLPYQDIHDILESDLAEPPFLLV
                     LDGVEDPHNLGACLRVADAMGVHAVIAPKDRAAGLNATVRKVACGAAETVPFIAVTNL
                     ARTLRELKEAGVWLVGAVAEAEQDLFSATFKGPLAMVLGAEGTGLRRLTAETCDQLVS
                     IPMFGMVESLNVSVASGICLYEARRQRHTG"
     gene            17130..17411
                     /locus_tag="PKW44_00755"
     CDS             17130..17411
                     /locus_tag="PKW44_00755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR50149.1"
                     /translation="MALKIDAKKVALALLLSCAGLLQAAMAAEAQQVLASDPEAGEGP
                     VATPAPKHASARPHRKARSHLTSRSRLDARECLDQESDKAIISCAERFR"
     gene            complement(17430..18092)
                     /locus_tag="PKW44_00760"
     CDS             complement(17430..18092)
                     /locus_tag="PKW44_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020182573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HQR50150.1"
                     /translation="MRQVNQYRTLVFDCDGVILDSNKVRAQAFYNCALPYGDRHAAAL
                     REYHILHGGVSRYVKFEVLLRDMVGVPVTDEAMQALLHAFTTEATIGLLQCEIAPGLQ
                     DLREATPHANWILVSGADQQELRHVFAQRGIAAWFDGGIFGSPSNKDDILARELAGGN
                     LRKPGLFFGDSRYDHQAAARAGLDFVFMSEWTDMADWQGYCREHGIAAAASFDQFMAA
                     QR"
     gene            complement(18080..18655)
                     /locus_tag="PKW44_00765"
     CDS             complement(18080..18655)
                     /locus_tag="PKW44_00765"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019916599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HQR50151.1"
                     /translation="MNNVPLAQAYRLLNHGPTVLVTSAHGDRRNVMAAAWIMPLDFDP
                     PRVAIVIDKKTYTRELIDASGSFAINVPCRAQADMVVQVGSGSGRELAGKQSGDKFAA
                     FGVPTFAATNIDAPLVQGCVAWLECRVIPEPHIQQAYDLFLAEVVAAQADERVFSNGR
                     WHFDGHDELRTIHHVAGGAFLSSGEAFACAR"
     gene            complement(18648..18938)
                     /locus_tag="PKW44_00770"
     CDS             complement(18648..18938)
                     /locus_tag="PKW44_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HQR50152.1"
                     /translation="MPRLVYTARALADLERVVDFLQQNDPAAAMQTYDLIESALNILT
                     RHPLVGRPAEQGLRELVISRGATGYIALYRFNEALDEAMVLAVRHQREAGYE"
     gene            complement(18938..19219)
                     /locus_tag="PKW44_00775"
     CDS             complement(18938..19219)
                     /locus_tag="PKW44_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR50153.1"
                     /translation="MPATTTLKLPDTLKSRIAPLAQAEGKTAHAWMVAALETQAALAE
                     KRTAFVAEALAAEQEALQSGEVYAAEDVHRYLRERAAGGKPARPQTIRR"
     gene            complement(19338..19868)
                     /locus_tag="PKW44_00780"
     CDS             complement(19338..19868)
                     /locus_tag="PKW44_00780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR50154.1"
                     /translation="MKIRTIRNQNEVDELVATIARSTEYARTQLARIAAENDGLQALW
                     SIKFSPMGCNPLDFGSPLNLIEQVNQTFTYLASAKAVQLLLELHPQHAPFTVNLGTSP
                     GSDIESENGTLAAEVFAAVNTSNNKKLAKDRAKVSKTNAQLKYVFFMCPDYEAGRQQN
                     LEREAGVMVWSLGTAP"
     gene            complement(19891..>21505)
                     /locus_tag="PKW44_00785"
     CDS             complement(19891..>21505)
                     /locus_tag="PKW44_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HQR50155.1"
                     /translation="RIRTRFPPEPNGYLPTGHAKSICLNFGLARDYDGVCHMRFDDTN
                     PEKEEQEYVDSITEMVHWLGFGWEKYGHSHLYYASNYFDFMYRAAEALIEAGLAYVDQ
                     QTADEMRANRGTLTEPGKNSPYRDRSKEENLNLFREMRDGKHADGSMVLRARIDMASP
                     NINLRDPAIYRIKRATHHNTGDKWCIYPMYTYAHPLEDAVEGVTHSICTLEFEDQRPF
                     YDWVLEKLADVGLLAHPLPRQYEFARLNLTYVVLSKRRLIELVQDKYVNGWDDPRLPT
                     LAGARRRGYTPEGFRLFAERIGVSKADSWIEYTILEDCMRETLNASAERRIAVLDPVK
                     LVIDNYPESHSEDCHAPNHPQKPELSKRVVPLSRELWIEREDFMEEPAKGYFRLYPGN
                     KVRLRYGYVVECTGCDKDAAGNVTTVHCTYLPDTKSGTPGADSIKVKGNIHWVSAQHA
                     YAAEVRLYDRLFKEANPGSNDRNYLDDLNPDSVRTITAQLEPALKEAQPEDRFQFERH
                     GYFVADRRDSQPGKPVFNRTVTLRDAWQK"
BASE COUNT         3787 a         6433 c         7077 g         4208 t
ORIGIN      
        1 gctcgctttc acgcgggccg gggttttcgg gggtgtggcg tgtggcttac ttggacagca
       61 atttggtgat ctgggcttca gcttctgtcc agaagaccgt ggggtcgccg gcaaaaccgt
      121 tgcgctcggc gatgtagtag gctgcgactt cgaccatgcg gtagcgctct tcggcacctc
      181 gcgcagccac tacctttttg gcagcagcgg gcttcttggc ggcagcggcc ttgggagcag
      241 cggccttctt cggagcagcg ggtttcttgg ctgcagcagt cttgggagcg gcaacgctac
      301 tctttttggc agcagcgggc ttggcggcgg gggctgcctt ttgggcggcc ggtttggttt
      361 tggcttcagc catcttggtt cctttcggtt gcgaaaaact cacagaaaac gacaccgtaa
      421 tgtgtcaatg ttgcaatggt attaacaaac gcatgaaaaa tcaaacggtt ccgccaacgg
      481 taaggccgtc gatgcgcaaa gtgggctgtc cgacccccac cggaacgctc tgcccctcct
      541 tgccgcaggt gccgacgccg ctgtccagcg ccatgtcgtt gccgatcatg gagacgcgcg
      601 tcagcacgtc cgggccgttg ccgatcaggg tggcgccctt gaccggatag gtcagtttgc
      661 cgtcctcgat catccacgcc tcggctgccg aaaacacgaa cttgccgctg gtgatgtcca
      721 cctggccgcc gccgaagttg accgcataca ggcccttctt caccgagcgg atgatctctt
      781 ccggcgattt gtcgccgttc agcatcacgg tgttggtcat gcgcggcatc ggcaggtggg
      841 cgtaggattc gcgccgcgcg ttgccggtaa gtgctgcgcc catcaggcgg gcgttgagcg
      901 aatcctgcaa atagcccttg agcacgccgt tctcgatcag cacggtgcgc tgggtgggcg
      961 tgccctcgtc gtcgatgttg agcgatcctc tcctgtccgt gatggtgccg tcgtccacca
     1021 cggtcacgcc atcggaggcg accttctgcc cgatgcggcc ggagaaggcg gagctgccct
     1081 tgcggttgaa gtcgccttcc agtccgtgcc caatcgcctc gtgcagcaaa attccgggcc
     1141 agccggaacc gagcactacc gtcatggccc cagccggagc ggggcgcgcg tcgaggttgg
     1201 tgatggcctg gtgcacggcc tgttttgcgt agccgtgcag cacgtcgtcg ctgaaatagc
     1261 cgtagtcgaa gcgtccgccg ccgccggaag agccctgttc gcgcttgccg tcggcttccg
     1321 cgatgacttg tatcgaaacg cgcaccagcg gccggatgtc ggccgccatc atgccgtcgt
     1381 ggcgtgcgat cagtaccacc tcgtattcgc cggccagcga ggccatgacc tgggtgacgc
     1441 gcgggtcgag ttcgcgcgcg tatttttcca gacgctccag cagtctgacc ttggagtcgt
     1501 cgctcatgga ggcgatgggg tcgtgcggca ggtagaggcg cggcgtatcg ctgcgctgca
     1561 tggcctgggc actgcgttcg ctgccctgcg cggcgatggc gcgggtggcc agcgccgcct
     1621 gctgcagcgc cggcaggttg atgtcatcgg agtaggcaaa agcggtcttt tcgccgctga
     1681 cggcgcgcac gcccacgccc tggtcgatgc tgaagctgcc cgacttgacc tggccttcct
     1741 ccagccccca gctttccgcg cggctgtact ggaaatacag gtcggcgtaa tccaccttgt
     1801 gcgacatgat gctgcccagc accccctgca gcgcgtggtg gtcgagctgg taagggacga
     1861 gcaaggtgtc ggcggcggtg gtgaacaggg tgccaggttc cgggctggaa gcggaaggga
     1921 gggtttctgg tttcatggtg cgattctcgg ttagcagcgc ctattgtaag ggaaaggggt
     1981 ttcagtaggt tatgattggc aatccgcgta accgttccct gttgactcgt attcatgacc
     2041 gattcccgcc tgccgccagc gattttcctg atggggccga ccgcttccgg caagaccggc
     2101 ctcgccgtgg atctggcgca gcggctgccg gtggaactca tcagcgtcga ttccgcgctg
     2161 gtctaccgcg gcatggacat cggcacggcc aagccggatg ccgccacgct ggcgcgggcg
     2221 ccgcaccacc tgatcgacat catagccccg accgcatcct actccgccgc gcagttccgc
     2281 gacgacgccc tgcggctgat gggcgagatt acccagcgcg gcaacatccc gctgctagtg
     2341 ggcggcacca tgctctattt caacaccctg cagcacggcc tcaacaacct gccgcaggcc
     2401 gatcccgtcc tgcgcgccga gatcgacgcc cgcgccgagc gcgaaggctg gcccgccctg
     2461 cacgcagagc ttgctcaggt tgaccccgcc accgccgcac gcctgatgcc caacgacgcc
     2521 cagcgcatcg aacgcgcgct ggaaatctac cacctcaccg gccgccccat gtcggaattg
     2581 ttgcgggaag aggaacgcgc cagcttgccc taccgattgc tgaaaatcgc tctgctgcct
     2641 tccgaccgcg ccgtgctgca tcaacgcatt gccgaacgct tcgacgccat gctgcaggct
     2701 ggtttgctgg acgaagtggc gcgcttgcgg cgcgaggttc cggctctgag cctcaacctg
     2761 ccttccatgc gctgcgtcgg ctaccgccag gcctggcaac accaggacgg cgaattcgac
     2821 ctcgccaccc tgcgcgacaa gggcatcgcc gccacgcggc aactggccaa gcgccagatg
     2881 acctggctgc gcggcatgga tgacgtggag gaactggatt gcctcgaatc agggctggcg
     2941 gaggagctgg aacagcgggt gcgggagttt atagggtagg ttggtcaaaa catggtggcc
     3001 cgacttgctc agtttcatgc taactaatga ccgcaccatt gctgttcgca cggtacgcca
     3061 tcattgttac catccatctg tgtaccggga caattctgca agaaaaacac tgcctcagta
     3121 caggaagtca tttgcgagca atgggtacga ccatcacagc tataattaat tgcgagttct
     3181 ggggggggtg taaatctgtg gcatcaggag gttctgcact ttcggtactg tagtcagctg
     3241 caggttcgct gaatttgtgc atatggctgc cgtaaccata tacacctacg gcgcccagaa
     3301 tgaacagtgg cacgataaga cctcttagcc catgtttttc atgatggcgt gttggtacgg
     3361 gttgatctct gtaacttgca ttggctcgaa gaggacgtga aactgatttt gctcgttttt
     3421 tcccatcgtt atctatttcg atttcaaaag ataatgattc cccaatatgg ggtcgctgac
     3481 cgtccttcgg gaaaactgag atgtggacaa agatctcctg cccaccttgt gttggagtga
     3541 taaacccaaa accgcgctca tcattccact tggtaagttt tccattaatt tgcataattt
     3601 aatctgaatt aggagaaact tgttttctgc attcgcttcg ctgcatctac acattcttgc
     3661 agtgaagcca ccagagcgat acgcacgaac cccgctcccg ggttgacccc atgcgcctcg
     3721 cgcgcaagga agctgccggg caggacggtg acgttgcagt cgcggtagag gcgccgggcg
     3781 aattcggcgt ccgagccctt gaccctggcc cacaggtaga acgaggcgtc cggcagttcc
     3841 acgtccagca cgtttttcag catgggggtg acggtgcgga atttctcggt gtacagccgg
     3901 cggttctcga ccacgtgcgc ctcgtcgttc caggctgcgg cgctggcggc ctgcacggcg
     3961 gggctcatgg cgcagccgtg gtaggtgcgg tagagcagga atttttccag gatggcggca
     4021 tcaccggcga cgaagcccga gcgcatgccc ggcacgttgg agcgtttgga gaggctgctg
     4081 aacatgacga tgcgttccca gttgcggccc agctggtggg cggcctgcag ggcgccgagc
     4141 ggggcgtggc cttcttcgaa atagatttcc gaatagcatt cgtcgctggc aatgacgaag
     4201 ccatatttgt cggccagggt gaagatttcg cgccagtcgt ccaggttgta gaccttgccg
     4261 gtggggttgc ccggcgagca tacgtacatg agttgggtgc gctgccatac ggcttccggc
     4321 accgcgtcga agtccatgcg gtaaccatgc tccggcaggg tgttgaggta ccagggttcg
     4381 gccccggcca gaagcgcggc gccttcgtag atctggtaga acgggttggg actgagcacc
     4441 accggcttgt ttttgcccgg gtcgatcacc gcctgtgcga aggcgaacag cgcttcgcgg
     4501 ctgccgttga ccgggacgat ctgcttttcg atgtccggcg tggggatgcc gtagcggcgt
     4561 accagccagt cggcgatgga ttgccgcagc tccacgctgc ccagcgtggt ggggtagctc
     4621 gccagcccgc cgagattggc cgtcagtacg tccttgatga actgcggcgt ggcgtgcttg
     4681 ggctcgccga tggacaggtt gaccggcttg agcgccgcat tgggcgtgat gccggcgaac
     4741 aggtcgcgca gcttctggaa cggatagggg tggagcttat cgagattggg gttcatggcg
     4801 gcttcgatgg tcagcttgca ggaggggcgc cctcgccccg attgcgttac ggctcattgc
     4861 cgggttggtc gcgccgaggg cggcgctcct acacaaatca tgcctcgcat attggattcc
     4921 ggctcaggcc attatatccg ggcgctggcg gcgtcgatgg cggcgcgcag ttccgcgtag
     4981 ctgttggtga ccgggaactg cgggaattcg tcgatcacgt tctgcggcgg atggaacagg
     5041 atgccggcgt gggcctcgcc cagcatggcg gtgtcgttgt aggagtcgcc ggcggcgatg
     5101 accttgaagt tgagctcctt gaagcgctgc actgcctcct ttttctggtt gggcatgcgc
     5161 agatggtagt tggccagaaa gccggcgccg tcggtttcca gcttgtggca gaacagcgcg
     5221 ggcatgccca gctgcgccat cagcggcatg gcgaactcgt agaaggtatc ggacaggatg
     5281 acgacctgga agtcgcggcg cagagcgtcg aggaattccc tggcgccttg catcggcccc
     5341 atttcggcga tgactttctg gatgtccgcc aggcccagct tgtgttgctt gagcaggtcg
     5401 aggcggtagg tcatcagctt gtcgtagtcc ggctcgtcgc gcgtggtgcg ggaaagctcg
     5461 gggatgccgg tgcgcttgga aaactcgatc cagatttccg ggacgagcac gccttcgagg
     5521 tcgagacaga cgagttgcac aacattctcc tgataaaggc ggggctttcg ccgcgctgaa
     5581 aaaccgcaag tttaccgcgt tgcgacatgg ccgctcaagc gcgggaggcg gcgcacctgc
     5641 cgaaactttc tgctacagtt gccttcatgt ccaagcctga tcgctccatt cccaccttcg
     5701 gcctgctatt cggcgccacc gtctggggca tcatctggta cccgtaccgt ctgctggaac
     5761 aggccgggat ttccggcgtg gcctccagcc tttacaccta ttcgatcgcg ctgatgctgg
     5821 gggcgctgct gttcgcgctg cgccggcgcg gcctgactgt tgtgccggcc agtatcttct
     5881 ggctggcgct ggcggcgggc tggaccaacc tcagctacgt gctggcggtg atcgacggcg
     5941 aggtgatgcg cgtgatgctg ctgttctacc tctcgccgtt ctggacgctg ctgctggcgc
     6001 gcctgatgct cgacgagcgg gtgggattgg tcgggaccgc agtggtcgtg ctgtcgctgt
     6061 gcggcgctgc gttgatgctg tggcagccgg agtccggcct gccgctgccg cagaatactg
     6121 ccgagtggct ggggctgtcc tccggcatgg gcttcgccct gaccaacgtg ctcacgcgca
     6181 agtccacgca cctcacgctg caagccaaat cgctggcggt gtgggtgggg gtggtggcga
     6241 tggcgctgct gttcctgccg ttcgaatcgc gtcccttgcc gctgcccggc gaagtggggc
     6301 tgtcaggcct gctgctgatg ggcggcatcg cgctgctact gatgctggcg acgctgctgg
     6361 tgcagtacgg cgtgacccac atgccggtga cgcgtgcctc ggtgattttc ctgttcgaac
     6421 tggtggtggc cgccgtgacg tcgtatttcc tggcgcacga ggtgctcagc gtacgggagt
     6481 gggtgggagg cgcgatgatc gtggcggcgg cgctgttcgc cgcgcgcatg gaagaacatg
     6541 cctaatccgc tgttcttgca ccttcccttg ccaggcggat cttgagcgag acgtcgttgc
     6601 gcgaatcggc atagtaaacg gcgttctcct cgtcgatctt gccttcctgg aacagctgga
     6661 acagcgactg gtcgaaggtg cacatgccga tgtcgttgct ggaggacaag acttgcttga
     6721 gctcttccag cttgcccttc atgagcatgt cggagacgta gtggttgttg agcatcactt
     6781 ccatcgccag cacgcgcttg gcctgggtgc cgggaatgag gcgctgggac atgatgccgg
     6841 ccagattgag cgccagcgac agcaggatgg actgcttcat gtccggcggg cagaacgtca
     6901 ggatgcgctc gatcgcctgc gtggcattgt tggcgtgcag cgtggccagg cacaggtggc
     6961 cggtttcggc ataggcgatg gcgtgccgca tggtgtccat gtcgcggatt tcaccgatca
     7021 ggatcgcgtc cggagactgg cgcatggcgt tgcgcagtcc gttttcgaag gacttggtgt
     7081 cgatgccgac ttcgcgctgg tcgacgacgg atttcttgtg gttgtggatg aattcgatcg
     7141 ggtcttccag cgtcaggata tgcccggtct tgttttcatt gcggtagttg atcatcgcgg
     7201 ccagcgtggt gctcttgccg gaactggtcg agccgacgac cagcaccagc ccgcgtgggc
     7261 gcacgctgac ttctttcagc agcatgggca ggccgagttc ctccagcgaa gggatcgcgc
     7321 tcttgatgtg gcggaccacc atggaaactt cgccgcgctg caggaaggcg ttgatgcgga
     7381 aacgccccag gtcgctcacg ctgtgtgcaa aatttacctc cagcgtctcc tcgaattcgc
     7441 ggatcagggc ttcgttcatc agtgaatagg cgatttcctt gaccatgccc ggcggcatga
     7501 tctgactgtt tatcggcatc gcgatcccct cgatctcgat gttgatcgga gcgccggtgc
     7561 tgaagaacag gtcggatgcg ttcttttcca ccataaattt gaggactggg gagatgtcga
     7621 gagcggccat gaatgtgtcg gtcagaattg aaagtgaaaa aactttaaca caaaatcatt
     7681 gcggttcagc caagttgatg gcttgcggcg gctggcctga cgcatatcgc tgccacatcg
     7741 cgcggtaagc ttgttgcaga tggcgggcga aacgcggcgt atcgaacagc ggcgcagtgt
     7801 cacactgctg cgccagcacg gttcgcaatg catgcagttc gtcgggcgat gttgccaggc
     7861 gcagggcaag cgcctgatag tcgtgcaggt ttccggtgac cagttgggga aatccggctg
     7921 ccctgagcag gctgccggcg acgcgcgcgg caaaagtttc gccggcgcag gtgaccagcg
     7981 gcagccccat ccacagcgcg tcgctggtgg tggtatgggc gttgtagggc agggtgtcga
     8041 ggaacagatc ggcgcagcgg tggcgcgcca ggtgctgctc gatcggcacg cgcggggcga
     8101 agatcaggcg gcccggttcc acgccgcgcg ccgctatttc gcgccgcagg ttggccttgg
     8161 cccaggggtt gcactccagc agccacagca cgctgcccgg cacggactta agcaagccta
     8221 gccagagacc ggacagttgc ggcgtgatct tgaaggtctg gttgaagcag cagaacacga
     8281 aaccatgctc cggcaggccg cattcggcac ggctgggggt agccgccaac gggcgctggc
     8341 ggtcgttggg ctggtaggtg tcgggcagca gcgccagttg ttcggcgtag tgctgcgcct
     8401 gctccggcgg cgtgatgaag ctgtccgaaa tcaggtaatc cacgaacggc gcgcccatgc
     8461 tgccggggaa gcccagccag ttgacctgga gcggcgccgg gcgcagcgcc aggatgccgc
     8521 tgcggctcga atgggtgaag ccggtgaggt cgaccaggat gtcgatgccg tcggcgtgta
     8581 tttttcgcgc cgcctcgtgc atggaaagat cgcggatgtc gcggaaatgg tcgaacgcct
     8641 gttcccagcg ccgccgcgcc gggctgtggt cgtcggggcc gtaggaatag ccgtggacct
     8701 cgaaggattc ccggtcatgc agttccatca gttccgtcgc cagccaggcc agcgggtgct
     8761 ggcggaagtc ggcggaaagg tagccgatct tcagtttgaa cttattttcc ctcgtgtgcg
     8821 ggtaagccag gcgcttgcca tcctcgacca gttgcccgag gcggttttcc gcccagtgct
     8881 ccgcacacag acgctgctcg gccgctgtcg tcccgggcaa ggcgaggaag gcgaacggcg
     8941 aaatttgcgc gcgcggctct tcgcgcacca ggcgccggat ttcggcgatt tcgtcgtcca
     9001 tgccggccca gtcgcagacg tgctggcgct ggtgcagcaa atgcatgcgc gcatggtgca
     9061 gtccggggtt aaggcgcaac gcctcgcggt agcgggcgat ggcttcatcg agcttgccca
     9121 tctcgcgcag cacgttgccc aggttgttgt ggctatcggc gtcgcgcggg tcaagcttga
     9181 gcgcggcgcg gtagctggcc gccgcctgct ccggctggcc catctcgcgc agggcgttgc
     9241 ccaggttgta atgcgctgcc ggctgcgccg ggtcggcccg cagcgcggcc tgaaactgcg
     9301 acacggcatc ggcgtagcgg ccctgcgcgg agcgcgcgtt gcccagctgc agcaggatct
     9361 gcgggtggcc gggggccagt cgcagcgcgg cgaggaaact gctctcagcc tctgctggcc
     9421 gtcgttgacc cagcagcagg ttgccgagat cgtagtgcaa ctcggggtct tgcggtgcgc
     9481 gcctgatagc tgattccaat tgttcgattt gccggtcttg catgatgccg attctaaccg
     9541 atcgccgcgc cgatcaaccg cttcaggccg cgctgccgca gcaggaagcg gttggggtcg
     9601 agcgcgcgcg ccagcgtggc atcgaccggc agaggtgcat tttccagcat ggcggcgatg
     9661 agctcggcgc acagcggtgc agtgagcagc cccttggagc cgtggccggt gttgacgtac
     9721 aggctatcca gccagggcag gtgttccacc ggcaggcggc tgcccaggct gtagcgctcg
     9781 gctaccggtc cggcatccag cagcgggccg gccagcggca ggtaatccgg agcgacgcag
     9841 cgcagcgccg cgcgtccatg cagccgggcg gcatccagcg cagacccacc cagcgcctga
     9901 tgcagtgcgg gggagaggtg tttgagcatg gccaggtttt ccgcgtggtc gtcggcccgc
     9961 aattccagcg agtgttcgtt cggggcgaag gtagcgccgg cgcagtgcgt tccctggcgc
    10021 gccgggctga tgtagccctc ggtgcagact accgcgctga gttttgcgct ggccggcgtg
    10081 gccggcagct gggtgatctg cccacgtacc ggctccagcg gcaggtgtgc ggtctgcggg
    10141 aatgccgtgc tgtcgttggc gctgcaaacc acgaccacgg gcgcttcggc caccaggtca
    10201 tctgcatcca gcacctgcca gccgtggtcg ccgtgcagca gctgtagcgc ctgagccgag
    10261 gtcttgagcg taatgttggg atgcgccgcc agcgcgcggc acagtgcggg cggatgtacc
    10321 cagccgccgc caggaaacca cagtccgccg tgcggcatgg cgataccggt cagcgctgcg
    10381 gcttccccga catcgacggc gcgtaccaga tcaggcggca ggccgcgtgc cagcacctct
    10441 tcgcagcgct tggcctcgcg cgcgttgaag gcctgttgca gcaggccgca gtcgtcccag
    10501 tcctgcccct tgggcaacaa ccgttgtaac aggcgcagtg tagtcatgaa gccggtctgc
    10561 gccacccggc tcaaggggat gtcgtgtccc gacaggcgcg gatagagcac gccctgcggg
    10621 ttgccggagg cttcctgcgc cagctcgcca tgacgttcga tcagcgtcac ctgccagcca
    10681 cgactggcca ggctccacgc gctggcggcg ccggcgatgc cgccgccgat gaccatggcg
    10741 tgtttgtccg ggagcgccgg ctggctggtt gtgccgccgg catactctcc gcacagcatt
    10801 tcgcgtttcg tgccgaatcc tgctaccttt tctacgttga atccgctcgc ttccagcccg
    10861 cgccgcaccg cgccggcgct ggtaaaggtg gcgaaggtac agcccggaac cgatagccgg
    10921 gcgatttgcg cgaacagttc cggctgccac atctcggggt ttttggcggg ggcgaagccg
    10981 tcgagaaacc aggcgtcaac gcttgcccgc agttgcggca atgttgccct tgcatcgccg
    11041 atcagcaggg taagggtgac gcggccgtcg tcgaattcca tccgtctcca gcccggggcc
    11101 agccagcggt attgctccag caactgcgcg ctcaacccgg acagctccgg ccacagcgtc
    11161 agcgccttgg ccaggtcgtc ggaagacagc gggaattttt cggtactgac gaaatgcagg
    11221 cgggcatcgc tcggggcgca ttctctccac aactgccacg cgcagaggaa attgaggccg
    11281 gtgccgaagc cggtttcggc aatggtaaac aaaggctggc gcaaaccatg ccagcgcccg
    11341 gccaggcgat tatgttgcag gaacacgtgt cgtgtctcgg cgatgccgga ttcgcgcgag
    11401 aagtagacgt cgccgtagat tgccgaaatc ggctggccgt cccgccactc cagttttgtt
    11461 tgtgttgatg tcatggatgg tgcatatttt cgcatgagat gacacattca tccgacgcct
    11521 gtccttcttc gtgggtgacc cgcttcgcgc cgctgatccg acaaggcggc agcgtgctcg
    11581 acctggcctg cggtggtggt cgcaacgcgc gctggctggc gcaacagggc tggcgggtgg
    11641 aagctgtgga tcgtgacccg gctgcgatcg ccggcttgcg tggggtaaaa aatatcagcg
    11701 cgctgcaggc cgatctggaa gtcgcgccgt ggccgtatcc gggccgcaaa ttcgacggcg
    11761 tcgtggtctg ccgctacctg taccggccgt tgctgccggt actggcggca agcctggccg
    11821 agggcggcgt gctgatttac gaaaccttca tgctggggca ggaacgatat ggccgaccca
    11881 gcaaccctga tttcctgttg cgtcctgatg aattgctgga agcctgctca ggggctttgc
    11941 aggtcatcgc gttcgagcag ggaattttca atgaaaccaa tcctgccatg ctgcagagaa
    12001 tttgcgcgcg gcgctgattt ttacgatgta tactgactag tatgtttacc aaggatatcg
    12061 ccatgtaccg acgcctgttt ataactcttt cggctgctgc cctgcttgct tcctgtgccc
    12121 accagccggc ttcgcacgat gccgcggaat ggccgagcta cggcagggat tacagcaacc
    12181 agcgcttttc ccggcttgac caggtcaacc gcgacaatgt cacccggctg gcgccggcct
    12241 ggacctatca gagcggcgtc aaggccactt tccagaccac gcccatcgtg gtggagggcg
    12301 tgatgtatgt ctcgctgccg tataaccacg tggtggcgct ggatgcgagg accggtcagc
    12361 agctgtggaa atacgagcac cagcgccgca aggactggaa gatgtgctgc ggaccggcca
    12421 accgcggtgt ggcggtgagt ggcggaaaag tttacatcgg caccgtggac gcgcgtctgg
    12481 tggcgctgga tgccaggacc ggcaaggtag tctgggatat cgacgcggtc agcgaaaacg
    12541 tcgtcacgga aggcatcgac gccctgcacg ccgacgaccc cttgagcaag gaacgggtgt
    12601 cggggggtac cggtatcggt atcgccatgg cgccggtggt gtaccagggc agggtcatcg
    12661 tcggcatcac cggcgtcggt tacggcctgc acatcgagga gaatcgcccg gatgccccct
    12721 tgggcgcggt gatcggtgtg gccgggcgct atggccgacc gggcttcctg gcggcgttcg
    12781 acgcgcagac cggcaagcgc gtgtggcagt tcgacaccat cccgccgcaa ggctgggaag
    12841 gaaagttcag cccgaccacg ccggacggcg tgccgctcaa ccgcaatatc gaacaggagc
    12901 gcgccacctt cacgcaatac caggaggcct ggcgctatgg cggcggttcg gcctggacca
    12961 cgccggcgat tgatccagaa ctgggcctgc tgtatttcgg caccggcaac ccctcgccgc
    13021 agatggatga cgtctcgcgc cctggcgaca acctctacac tgtctcgttg gtagctctgg
    13081 aagccgccac cggaaagctg cgctggactt atcagcaggt gccgcacgac gtgtggggct
    13141 acgacctcgc cagcccgccg atactgttcg actaccagca cgacggcaac agtatcccgg
    13201 cggtggggca ggctagcaag accggctggt tctacgtaca tgaccgtgcg accggggaac
    13261 tgctgctgaa gtccgaggcc ttcgtgccgc agtccaacct gttcgcccgc gccacgccgg
    13321 aaggcacgcg catccatccc ggcgtattcg gcggcgtcaa ctggtcgcct gccgcggtgg
    13381 atgaagcccg ccagcaggtg tttgtcgctg caatgcacat gccggtgcgc tatagcctgc
    13441 acgaaacgcc tgcaacggat ggcaagccgg ccatccgcta caccgcgtcg gaaccggcgg
    13501 atgagccgaa gtggggcttg ctgtcttcga tagacctgaa aaccggcagg atccgctggc
    13561 agcacaagac gccggaaccc ttggtaggcg gcgtgctggc gaccgccggc ggggtgctgt
    13621 tcaccggcga aggcaacggc agttttgacg ccttcaacag cgacagcggc gatctgttat
    13681 ggcagtacaa ggcggaagcg ggcgtaaacg cgccgccgat cacctatgaa ctcgacggcg
    13741 tgcaatacgt ggcggtggct gccggtggta accagatctg gggctacaag cagggggata
    13801 cgctgctggt gttcaagctg gacaacagca gcggccggtg attggaaagg aaaatacccg
    13861 cagtcgatgg gcaggctgga gtaaaatgct tcagtttgga tcggcttgaa gtgccgcacc
    13921 ggttaatggc aacaaaaaac cgcacctgct gttaagcgtt ggtgcggttc ctgttgaaat
    13981 cgatggtgcc cagaagagga ctcgaacctc cacatctctc gatactagta cctgaaacta
    14041 gcgcgtctac caattccgcc atctgggctc gaaagagacg cgaattttat aggaaagact
    14101 gattttgtca acgagaaaaa aacagcgcgg aaaggatccg caagccgagc gcgaagcggc
    14161 caattacgcg catccattgc ccagccgcga attcatcctg caggtgatgg cggacgaggg
    14221 cgcgccggtc agcgtggaaa ggctttacga gttgctggat atacgcaagg gcgaacgcga
    14281 catattcaac cgccgcctgg gcgccatgga gcgcgacggg cagattttcc gcaaccgcaa
    14341 gaatgccctg tgcatcgcgc agaagatcaa cctgatcgcg ggcaaggtgc aggggcaccc
    14401 ggacggcttc ggcttcctca ttcccgatgc gggcggcgag gatctgttcc tgtcgcccaa
    14461 ggaaatggcg caggtactgc acggcgaccg cgtcatggcg cggcagtccg gcctggaccg
    14521 gcgcgggcgt cccgagggca agatcgtcga ggtgctggag cgcgccaaca agcgcctggt
    14581 cgggcgcctg atccgcgagg ccggcgtggt gctggtggcg gcggaagacc gccgcatcaa
    14641 tcaggatatc ctggttccgc cgggcaagga catgaaggcg caggccggac agatcgtgat
    14701 ggtggaactg atcgaacagc cgtcggccta tgcccagccc atcggcaggg tggtcgaaat
    14761 tctcggcaac tacgccgacc ctggtatgga aatcgaaatc gcgctgcgca agcaccagct
    14821 gccttacgaa ttcagcgctg ctgctgtgca gcaggcggag cagtacccgt ccaaggtgca
    14881 gaagaaagac tgggccgggc gcatcgatct gcgtgaattg cccttggtca ccatcgacgg
    14941 tgaaacggca cgcgattttg acgatgccgt ctattgcgag ccggaaggca agggctggcg
    15001 gctggtggtg gccatcgccg acgtcagctt ttacgtgaag ccgggcgacg cgctggacaa
    15061 ggatgccttt gaccgtggca actcggtgta tttcccgcgc cgcgtgatcc ccatgctgcc
    15121 ggaggcgctg tccaacgggc tgtgctcgct caacccagat gtggagcgca tgtgcatggt
    15181 gtgcgacatg caggtggacg gcaaaggggt gatcaagcgt tacaagttct acccctcggt
    15241 gatgcgctcc aaggcgcgca tgacctacac caaggtgtac gacatgatcg tcgacccggc
    15301 cgggaagacg gccagggaat atgcctggct gctgccgcac gtgcacaacc tgtatgccct
    15361 gtaccaggcg ctggccaagt cgcgcgagca gcgcggggcg atcgagttcg aaactatcga
    15421 aacgctgatg atgttcaatg accagggcaa gatcgaccgc atcgtgccat ccacgcgcaa
    15481 tgatgcccac cgcctgatcg aggagtgcat gctggcagcc aacgtctgcg cttcggattt
    15541 cctcaaggaa catcatcacc cggcgctgta ccgcatccac gaaggcccga ccctggaaaa
    15601 gctggagaag ctgcgcggtt tcatggcaga gttcggcttc gtgctgggcg gcggcgatgc
    15661 gccgcacgcc aaggattacg gcaagttgct ggcgcgcatc aagggccgtc ccgacgagca
    15721 gctgctgcag accgtgctgc tgcgctccat gcagcaggcg gtgtacagcc cggacaacgt
    15781 cggccacttt ggcctggcct acgagcatta cacccacttc acctcgccga tccggcgcta
    15841 ccccgacttg ctgatccacc gcgccatcaa ggcggtgctg gaaggcaaga aatacaaggc
    15901 cggcgactgg agcaacctgg gcatgcactg ttcgttgacc gaacgccgtg ccgacgacgc
    15961 cacgcgcgac gtggaaacct ggctcaagtg cttctacatg caggacaagg tgggcgagac
    16021 cttcaacggc accgtcgccg gcgtcaccgg cttcggcctg ttcgtcgcgc tggacgaggt
    16081 ctatgtggag ggcctgctgc acgtcaccga actgggcaac gactacttcc atttcgacaa
    16141 catgcgccac gaaatgatag gcgagcgcac cggcacgcgt ttccgcctgg gtgatcgatt
    16201 aagggtcaag gtggcgcgcg tggacatgga aaccagcaag atcgacttcg tgctggcgca
    16261 ggaagacgaa gcagagcaga aaaccgcgcc gaagaacggc ggcaagaagg gtggcaagaa
    16321 gaaatgagtg tttcgagaat cgtgttcggt ttccacgcgg tattgagccg catccggcag
    16381 aacccggcca gcgtgcagga aatctgcgtg gacaagacgc gcaaggacgc ccgcatgcag
    16441 gacctgctgc aactggcgga gcagaagggc gtgcgcgtga tgcagatgga gaaggaacgc
    16501 ctcgacggcc tcgccgccaa cggtcgtcac cagggcgtgc tggcgcgggt ggcggatacg
    16561 ccgctgccct atcaggacat ccacgacatt ctggaatccg acctcgccga gccgccgttt
    16621 ctgctggtgc tggacggggt ggaagacccg cacaacctgg gcgcctgcct gcgcgtggct
    16681 gacgccatgg gtgtgcacgc ggtgatcgcc cccaaggatc gcgcagccgg cctcaacgcc
    16741 acggtgcgca aggtggcctg cggcgcggcg gaaaccgtgc cgttcatcgc cgtcaccaac
    16801 ctggcgcgca ccctgcgcga actgaaggag gccggcgtgt ggctggtcgg cgcggtggcc
    16861 gaggccgagc aggatttgtt ctccgccacc ttcaaaggcc cgctggccat ggtgctgggc
    16921 gccgaaggca ccggcctgcg ccgcctcact gccgaaacct gcgaccagct ggtcagcatc
    16981 ccgatgttcg gcatggtgga aagcctgaat gtttccgttg ccagcggcat ctgcctgtac
    17041 gaagcgcggc gccagcggca taccggttga ataaccgcgc ccgctatggc acgatgtttc
    17101 tactgcgatc aacttcgagg gacggcaaca tggcgctgaa aattgatgcg aaaaaggtgg
    17161 cgttggcact cttgctgagt tgcgccggac tgctgcaagc tgcgatggcc gccgaagcgc
    17221 agcaggtcct ggcttccgat ccagaagccg gcgagggtcc ggtcgccacg cccgcaccca
    17281 aacacgcttc agccaggccc caccgcaagg cgcgcagtca cctcacgtcg cggtctcgcc
    17341 tcgatgcgcg cgagtgtctg gaccaggaat cggataaggc catcatcagc tgcgccgagc
    17401 gcttccgcta ggcgcacgcc tctggggcgc tacctctgcg ccgccatgaa ttgatcgaag
    17461 ctggcagcgg ccgcaatgcc gtgttcgcga cagtagcctt gccagtccgc catgtccgtc
    17521 cattcgctca tgaacacgaa gtccagcccg gcacgcgccg ccgcttggtg gtcgtagcgg
    17581 ctgtcgccga agaacaggcc gggcttgcgc agattgccgc cagccagttc gcgcgcaagg
    17641 atatcgtcct tgttgctggg gctgccgaaa atgccgccgt cgaaccaggc ggcgatgccg
    17701 cgctgggcga agacatgccg cagttcctgc tggtccgcgc ccgacaccag aatccagttg
    17761 gcatgcggcg tggcctcgcg caaatcctgc agccccggtg cgatctcgca ttgcagcaga
    17821 ccgatggtgg cctcggtggt gaaggcgtgc agcagtgcct gcatcgcttc atccgtcacc
    17881 ggtacgccca ccatgtcgcg cagcagcacc tcgaacttca cgtagcgcga caccccgccg
    17941 tgcaggatat ggtattcgcg cagcgccgcc gcgtgcctgt caccgtaagg tagggcgcag
    18001 ttgtagaaag cctgcgccct gaccttgttg gagtcgagaa tcacgccgtc gcagtcgaac
    18061 accagcgtgc ggtattgatt tacctggcgc atgcgaacgc ctccccgctg gacagaaagg
    18121 cgccgccggc aacatggtgg atggtacgca gctcgtcgtg cccgtcgaaa tgccagcgcc
    18181 cgttcgagaa cacgcgctcg tcggcctgcg cggccaccac ttcggccaga aacaggtcat
    18241 aggcctgctg gatgtgcggc tcggggatta cccggcattc cagccaggcc acgcagccct
    18301 gcaccagcgg cgcgtcgata ttcgtcgcgg cgaaagtcgg cacgccgaac gccgcgaact
    18361 tgtcgccgga ttgttttccg gccagctccc gcccggagcc tgagcccacc tgcaccacca
    18421 tatcggcctg cgcgcggcac ggcacgttga tggcgaagct gccggaagca tcgatcaatt
    18481 ctctggtgta ggtcttcttg tcgatgacga tggcaaccct gggcgggtcg aaatccagcg
    18541 gcatgatcca cgccgccgcc atcacgttgc ggcgatctcc gtgagccgag gtcacgagaa
    18601 cggtagggcc atggttgagc aggcggtagg cctgcgccaa aggcacgtta ttcatagccg
    18661 gcttcccgct gatgccgtac ggccaacacc atcgcttcat ccagtgcctc gttaaaacgg
    18721 tatagcgcaa tatagcctgt tgcgccacgt gagattacca gttcccgcaa accctgttca
    18781 gccgggcgac ctacgagggg gtgccgggtc aggatgttca gagcggattc gatcaggtcg
    18841 taagtctgca tggctgcagc gggatcgttc tgctgcagaa aatccaccac acgctccagg
    18901 tcggccagcg cgcgcgctgt atagaccaga cgcggcatca gcggcgaatg gtctggggtc
    18961 gggcaggttt gccgccggcc gcacgctcgc gcaggtagcg atgcacgtct tcggccgcat
    19021 acacctcacc ggattgcagc gcttcctgct cagcagccaa ggcttcggcg acaaaggcgg
    19081 tgcgtttctc cgccagtgcc gcttgcgttt ccagcgcggc aaccatccac gcatgcgccg
    19141 tcttgccttc ggcctgagcc aatggcgcga tgcgggattt cagggtgtca gggagtttga
    19201 gtgtggtggt ggcaggcatg gtgggtatcc agtgtcgtta agtgacacca aggtaacacc
    19261 gcgtttaaat agcgtcaacg ggggtgctgt cagggtttcc gaaatggcga tttgcgccgc
    19321 agggtatccg ccgtgaatca cggcgctgtg cctaatgacc aaaccatgac cccggcttct
    19381 cgctccagat tttgctgccg tccggcctcg tagtcggggc acatgaagaa aacgtacttc
    19441 agctgcgcgt tggttttgct gaccttcgcg cggtcttttg caagtttctt attgttcgac
    19501 gtgttgacgg ctgcaaaaac ttctgccgcc aaggttccat tttcagactc gatatcagaa
    19561 cccggtgaag tgcccaggtt tactgtgaag ggtgcgtgtt gtggatggag ttctagcagc
    19621 aactgaacgg ctttcgctga agcgaggtaa gtgaaagtct gattgacctg ctcgataaga
    19681 ttgagaggtg agccaaaatc caacggattg catcccatcg gcgagaactt tattgaccaa
    19741 agtgcttgaa gcccgtcatt ttcagcggcg attcttgcga gttgagtgcg cgcgtattcc
    19801 gttgaccggg caatagtagc taccaactca tcaacttcat tctgattacg aatggtgcga
    19861 attttcataa tacccaacga gtgacagatg tcacttctgc cacgcatccc ttaatgttac
    19921 cgttcggttg aacaccggct tgccgggttg gctgtccctg cggtcggcca caaaatagcc
    19981 gtggcgctcg aactggaagc ggtcttccgg ttgggcttcc ttcagtgccg gctccagctg
    20041 ggcggtgatg gtcctcaccg aatccgggtt gaggtcgtcg aggtagttgc gatcatttga
    20101 accaggattc gcctccttga acaggcggtc gtacaggcgc acttcggcgg cgtaagcgtg
    20161 ttgtgcggaa acccagtgga tgttgccctt gaccttgatg ctgtccgcgc ccggggtgcc
    20221 ggatttggtg tcaggcaggt aggtgcaatg cacggtggtg acgttgccgg cggcgtcctt
    20281 gtcgcagccg gtgcattcga ccacgtagcc gtagcgcagc ctgactttat tacccggata
    20341 caggcggaag tagcccttgg cgggctcttc catgaagtct tcgcgctcga tccatagttc
    20401 tctggacagc ggtaccacgc gcttgctgag ttccggcttt tgcgggtggt tgggggcgtg
    20461 gcagtcctcg gaatggcttt ccggatagtt gtcgatgacc agcttgaccg gatcgagcac
    20521 ggcgatgcgg cgctcggcgg atgcgttgag cgtctcgcgc atgcagtctt cgaggatggt
    20581 gtattcgatc caggagtcgg ccttggacac gccaatgcgc tcggcgaaca ggcggaaacc
    20641 ttccggcgta tagccgcgac ggcgggcacc ggccagcgtc ggcaggcgcg ggtcatccca
    20701 gccgttcaca tacttgtcct gcaccagttc gatcagtcgg cgcttggaca gcacgacgta
    20761 ggtcagattg aggcgggcga attcatactg cctgggcaac ggatgcgcga gcaggccgac
    20821 gtcggccagc ttctccagca cccagtcgta gaacggacgc tggtcttcga actccagcgt
    20881 acagatggaa tgggtaacac cttctaccgc gtcttccagc gggtgggcgt aggtgtacat
    20941 cgggtagatg caccacttgt cgccggtgtt gtggtgggtg gcgcgcttga tgcggtaaat
    21001 ggccgggtcg cgcaggttga tgttaggcga agccatgtcg atcctggcgc gcaacaccat
    21061 gctgccgtcg gcgtgcttgc cgtcgcgcat ctcgcgaaac agatttaggt tctcttcctt
    21121 actgcggtcg cggtaggggg agttcttgcc cggctcggtc aaagtgccgc gatttgcgcg
    21181 catctcgtcg gcggtctgct ggtcgacata ggcgaggccg gcttcgatca gtgcctcggc
    21241 ggcgcggtac atgaagtcga agtagttgct ggcgtaatac aggtgcgagt ggccgtattt
    21301 ctcccagccg aagcccagcc aatggaccat ctcggtgatg ctgtcgacgt attcctgttc
    21361 ttccttctcc gggttggtgt cgtcgaagcg catgtggcaa acgccgtcgt agtcgcgtgc
    21421 caggccgaag ttgaggcaga tgctcttggc gtggccggtg ggcaggtagc cattgggctc
    21481 gggcgggaat cgcgtgcgga tcctg
//
LOCUS       DAOMFA010000007        20800 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_150_length_20800_cov_5.137816, whole genome shotgun sequence.
ACCESSION   DAOMFA010000007 DAOMFA010000000
VERSION     DAOMFA010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 20800)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20800)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20800
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_150_length_20800_cov_5.137816"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            138..1508
                     /gene="hemN"
                     /locus_tag="PKW44_00790"
     CDS             138..1508
                     /gene="hemN"
                     /locus_tag="PKW44_00790"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HQR50156.1"
                     /translation="MEFSSELLHKYCKAGPRYTSYPTAPYFHEGFGPAQWEEELRESQ
                     NKGRDISLYAHIPFCDTLCYYCGCNMIATKDYSKAESYLEVLFKEIDRVAALTNPQRT
                     VRQLHWGGGTPTYLKPDDIRRLYAHIAGKFRIAPDAETGCEMDPRELTQEHVRALRQS
                     GFNRVSLGVQDLDAQVQHAVNRVQPEALIRQVYDWIREQGFHSVNFDLMIGLPHQTPA
                     SFEQTLDKVIAMGPDRFAVFNYAHVPWMKKHQKLIAEETLPQIDARLALQKLTLEKLT
                     AAGYVYIGMDHFARPDDELVKAQQAKTLYRNFQGYTTHKDCDILAFGASAISQTDEVY
                     AQNVKVLSEYRAMVQAGKLPVERGLRITHEDKLRREAITQIMCDLELDKASFGRQWDI
                     DFDTYFAAALDDLKGMQADGLVALEADKVRVTEEGRVFLRNIAMAFDAYLHQQASDKP
                     RYSKTV"
     gene            1528..3282
                     /locus_tag="PKW44_00795"
     CDS             1528..3282
                     /locus_tag="PKW44_00795"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HQR50157.1"
                     /translation="MWSEKLESALSGFLVMMTRRVRSMAAACGSLVLCTLLLMLAGNV
                     YAVEVPKAAPDPTALETDEVVPPADIVVYNRVIITLRAPAFSELPEVRARYAETRIKE
                     VLKGGGGDRVEVKRIPQGQMVQLNGRLAFIITPGDVDPNTQETPIQAAAEAVKKLEIV
                     IAETRESRSLDAILKDVALVLLGTAVFYALLRSVRWLRRYLLGWLARLASTEAERAKI
                     GGVQIFRLRGVVTVFRGMLATLFWMFTAVLIYVWLAYTLSRFPYTRYWGETLNTFILK
                     VLNGIGGAILGALPNLFVALVIVVLARLVVRMLHGFFDMVESRQIELRWIDADLATPT
                     RRIANVIVWLFALAMAYPYLPVANTEAFKGVTLLFGLMLSLGASGLVGQAVSGLILTY
                     ARVLRPGEFIHIAGNEGTVTETGVFATRVRTGMGEELTLPNTFILSNVTRNYSRAVKG
                     KGYIVDATVTIGYDTPWRQVHAMLIEAARRTPGVVENPLPRVYQIALSDFYTEYKLAC
                     HAGEEEPVPRAEVMSLLHANIQDVFNEYGVQIMSPHYFADPAAAKVVPKEHWHLAPAD
                     PDAPMGNIEVVGEKPKSR"
     gene            complement(3289..3630)
                     /gene="fdx"
                     /locus_tag="PKW44_00800"
     CDS             complement(3289..3630)
                     /gene="fdx"
                     /locus_tag="PKW44_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HQR50158.1"
                     /translation="MPKIIVLPHQELCPEGAVIEAKAGVSICDNLLDHDIDIDHACEK
                     ACACTTCHVIVREGYTSLPAPEEKEEDLLDKAWGLEPFSRLSCQAIVAQTDLVVEIPK
                     YSINMAKEGHR"
     gene            complement(3641..5506)
                     /gene="hscA"
                     /locus_tag="PKW44_00805"
     CDS             complement(3641..5506)
                     /gene="hscA"
                     /locus_tag="PKW44_00805"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HQR50159.1"
                     /translation="MALLQISEPGQSAAPHQHRLAIGIDLGTTNSLVATIRSGMSVVL
                     HDEQGRALLPSVVHYLADGGAEVGYTAQAQQSRDPHNTIVSAKRFMGRGVHDIADLGH
                     YPYRFVDAPGMLQLSTVAGSKSPVEISAEILRVLKARAEKSLGGELTGAVITVPAYFD
                     DAQRQATKDAARLSGLNVLRLLNEPTAAAVAYGLDNAAEGVFVVYDLGGGTFDVSVLR
                     LSRGVFEVLATNGDSALGGDDFDRRVFCWVLEQAKLPPIGPEDTRLLLTKAREAKEQL
                     TDHADTRITCMLSSGDLIDLTLTSQIFNDLTQYLVSKTLTPTRKALRDAKIGIEEVQG
                     VVMVGGATRMPQIQRAVAEFFRQEPLTNLDPDKVVALGAAIQADVLAGNKNDNELLLL
                     DVIPLSLGLETMGGLTEKIIPRNSTIPVARAQEFTTFKDGQTAMSIQVVQGERELVSE
                     CRSLARFELKGIPPMVAGAARIRVTFQVDADGLLSVSAREQTSGVEAHVVVKPSYGLS
                     DDEITNMLQSGFSHAEDDKQARALREAQVEAERLLEAIAAALGKDGALLTDTERAALE
                     QQMAALRGLAANNDARAISAGIEALNRASETFAARRMDASVKQALAGHKLDELKL"
     gene            complement(5506..6039)
                     /gene="hscB"
                     /locus_tag="PKW44_00810"
     CDS             complement(5506..6039)
                     /gene="hscB"
                     /locus_tag="PKW44_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0051259 - protein complex oligomerization
                     [Evidence IEA];
                     GO_process: GO:0097428 - protein maturation by iron-sulfur
                     cluster transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly co-chaperone HscB"
                     /protein_id="HQR50160.1"
                     /translation="MAPDFTQNHFELFGLPVSYAIELGHLDHSYRKLQGEVHPDRFAA
                     ATPAERLRSMQLATHVNEAYQTLKSPIARARYLLHMNGIDTQENTNTAMPAEFLMQQM
                     EWREAIEEAREADNIPALDRLLRELHNAAAAHQSHLHAMLDEHKDFASAAETVRKLRF
                     LDKVRTEIEQAIEALED"
     gene            complement(6096..6419)
                     /gene="iscA"
                     /locus_tag="PKW44_00815"
     CDS             complement(6096..6419)
                     /gene="iscA"
                     /locus_tag="PKW44_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017904917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HQR50161.1"
                     /translation="MAITLTENAAARVEKFLSNRGKGVGLRLGVKTTGCSGMAYTLEF
                     SDEILPEDLTFESHGVKILIDPKSLAYLDGTELDYTKEGLNEGFKFNNPNVKAECGCG
                     ESFTL"
     gene            complement(6422..6832)
                     /gene="iscU"
                     /locus_tag="PKW44_00820"
     CDS             complement(6422..6832)
                     /gene="iscU"
                     /locus_tag="PKW44_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HQR50162.1"
                     /translation="MAYSEKVLDHYENPRNVGSFNKDEKNVGTGMVGAPACGDVMKLQ
                     IKMGENGIIEDAKFKTYGCGSAIASSSLVTEWLKGKSLDEAMSIKNSDIAEELALPPV
                     KIHCSVLAEDAIKAAIADLRQKQGSSTETAACKG"
     gene            complement(6848..8065)
                     /locus_tag="PKW44_00825"
     CDS             complement(6848..8065)
                     /locus_tag="PKW44_00825"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0044571 - [2Fe-2S] cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HQR50163.1"
                     /translation="MTVRTPIYLDYSATTPVDQRVAEKMIPYLTEHFGNPASRSHVFG
                     WVAEKAVENAREQVAVLVNADPKEIVWTSGATESNNLAIKGAANFYAGSKGKHIVTIA
                     TEHKAVIDTVRELERQGFEATYLEPEPDGLVDLEKFKAALRADTVLASVMLVNNEIGV
                     IQDIETLGNLCRANGTIFHVDAAQATGKVEIDLEKLPVDLMSFSAHKTYGPKGIGALY
                     VRRKPRVRLEAQMHGGGHERGFRSGTLPTHQIVGMGEAFHIAREEMALENARIKRLRD
                     KLWAGLSEIEEVYLNGDLKQRVAHNLNVSFNFVEGESLIMAVKDIAVSSGSACTSASL
                     EPSYVLRALGRSDELAHSSIRFSIGRYTTEEEIDYTIDLLKAKIGKLRELSPLWEMYQ
                     DGIDINSVQWAAH"
     gene            complement(8076..9227)
                     /locus_tag="PKW44_00830"
     CDS             complement(8076..9227)
                     /locus_tag="PKW44_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HQR50164.1"
                     /translation="MSAEAVYLDHNATTLLDAKVLEAMLPFLRNQQGNPTSRHSYGRA
                     ARQAIDHAREQVAAAVGAHPSQVTFTSGGTEANNLAIKGIAATHTPSQVAVSAIEHPA
                     VSRPAHSLLGRGWKMAKIGVDAVGQLNPDQLVQALQQKTALVSVMAANNETGVLQDVP
                     AIAEVVRRHGSLLHTDAVQALGKVALDFNALGVNAMSISAHKIHGPQGAGALILDKRV
                     DIQPLLDGGGQEKGLRSGTENISAIVGFGLACELATRDLTSQAGRIGRLRDQLEQGLN
                     ELGGIIFGREAKRLPNTSFFAFPNIDGETLVMALDRQGYAVASGSACSSDSIEPSHVL
                     MAMGIAPGIARGAVRVSLGVDNSAAQIVAFLQTLKNELLRIKRMAAVAV"
     gene            complement(9248..9718)
                     /gene="iscR"
                     /locus_tag="PKW44_00835"
     CDS             complement(9248..9718)
                     /gene="iscR"
                     /locus_tag="PKW44_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HQR50165.1"
                     /translation="MRLTTKGRFAVTAMLDLALNEVDRPVTLSGISERQEISLSYLEQ
                     LFSRLRRNGLVKSVRGPGGGYRIAKKHEDISVSEIIRAVDEQIDATQCGGSEDCHGER
                     RCMTHDLWSSLNVKILEYLSGVSLAELVASQREGKKILIQEPKRREAQLQPIAA"
     gene            complement(9781..10524)
                     /gene="cysE"
                     /locus_tag="PKW44_00840"
     CDS             complement(9781..10524)
                     /gene="cysE"
                     /locus_tag="PKW44_00840"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HQR50166.1"
                     /translation="MFNHLREDIAVVFERDPAARTHWEVLTTYPGVHALIIHRFSHWL
                     WSLRLRWLARFSSHLGRFLTGIEIHPGATIGRRVFIDHGMGVVIGETAVIGNDCTLYH
                     GVTLGGTSWNKGKRHPTLENGVVIGAGAKVLGPITVGTEARIGSNAVVVKDVPAGTTA
                     IGIPARILDQDKVQQRNDMAQKMGFSAYAVSDDMNDPMVKAIHALLDHAAEQDKRLEE
                     VTQLLAKLGADTETDAEVGKAFDVNHPNK"
     gene            complement(10580..11305)
                     /locus_tag="PKW44_00845"
     CDS             complement(10580..11305)
                     /locus_tag="PKW44_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HQR50167.1"
                     /translation="MNKPESLANIRIVLCQTSHPGNIGAAARAMKTMGLHQLVLVRPK
                     KFPHPEAEAMASGATDILAQARVCENLQEALAGCVLVVGLSARKRELSHELITAHEAA
                     TLIAQAASSQHAALVFGTEMSGLSNAELIQCQRLAMIPANPEYSSLNLAAAVQVLTYE
                     LRMATDFAPAATDPSFPPASHDEIEGFHRHLEETLLRIGFLNPAMPKRLMPRLRRLFA
                     RAQLEKEEVNILRGILKFMQSPK"
     gene            11384..12199
                     /locus_tag="PKW44_00850"
     CDS             11384..12199
                     /locus_tag="PKW44_00850"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HQR50168.1"
                     /translation="MHPMLNIAVKAARNASAIITRASQDLGALKVRSKTYNDYVSEVD
                     HAAERAIIETLLDAYPNHAILAEESGASGSNAENLWIIDPLDGTTNFLHGFPQYCISI
                     ALKQRGQITQAVIYDPNRNDLFTATRGSGAFLNDRRIRVSTRTRLQESLIGTGLPFRD
                     FTHLDAYLAMFRDMIKKTSGLRRPGSAALDLAYVAAGWYDGFWEIGLSQWDIAAGSLL
                     VHEAGGLVGDFEGNETWLETGNIVAGNPKLFAQILQVLHPHLTEALKSANAGV"
     gene            12186..12548
                     /locus_tag="PKW44_00855"
     CDS             12186..12548
                     /locus_tag="PKW44_00855"
                     /inference="COORDINATES: protein motif:HMM:NF020908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HQR50169.1"
                     /translation="MLAFDAIAEQRICEAAARGELDGLPGTGRPLALDDDLLIPQELR
                     MAYRILKNAGYVPPEVQTLKEIGELERLIQSLDDGKQRSLAQRKLRLLTLQMNESRCG
                     SLQLEAAYYDKIVSQLAA"
     gene            12749..13147
                     /locus_tag="PKW44_00860"
                     /pseudo
     CDS             12749..13147
                     /locus_tag="PKW44_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611583.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            13194..13943
                     /locus_tag="PKW44_00865"
     CDS             13194..13943
                     /locus_tag="PKW44_00865"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HQR50170.1"
                     /translation="MAAWNSGKVVEHRQWSQTLYSLYVESDIEAFEAGQFVKIALEIN
                     GEVIGRPYSLVNPPDCRPLEFYYIEVPNGPLTSHLIKLIPGDNILVAPRAHGFMVLDE
                     VPQAKQLWLMATGTGIGPFLSILMTDKPWQRYEQVVLVYAVRTLSELSYQERITQILT
                     KHSGQFGFIPFLSREALDFALPGRIPQAIIDGRLEARAGIKISAENSQIMLCGNPQMV
                     KDITNILIERGLRKHRRFEPGQITSENYWQG"
     gene            complement(13972..14709)
                     /locus_tag="PKW44_00870"
     CDS             complement(13972..14709)
                     /locus_tag="PKW44_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR50171.1"
                     /translation="MKRSRLLSYEWDAIAGILAAMVAIVLHFLHVIDEQIILPVVLTL
                     MALLFINFMRHTRSNDHTAEQVEHIHKMVAKINSSLAVPDIVLIGPRQLRTANEQFAR
                     NMRGEATLFNVCLSMYRTQPLFDALLRPVIENPQVTSVQFVLDISQQGTWQTVVLPKI
                     ANSIAASKIKEPRWCSLDKNLSFILADTQPQGATEALLSFWGEPFMSQSTDRNVPRYI
                     LHVQAHSELLTQLVELDRNCHIHNSDS"
     gene            complement(14937..16154)
                     /locus_tag="PKW44_00875"
     CDS             complement(14937..16154)
                     /locus_tag="PKW44_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HQR50172.1"
                     /translation="MNLTDAWKTYTSAPHRMFFFGGAVQALVTLSWWLIDLGGRYGGW
                     YAPIAWALPPIDSHAFLMIYGFFPFFIFGFLMTTYPRWMNGEEVERRVYVPAFLLLAI
                     GTLLFYVGLILSFTLLKIALVLFLAGWGTGLTALLRVYLRAKHPDKRHAAITSVVLAL
                     GWLLVAGFVSDETHFVALAKVGGIWLLLLPVFFAVSHRMIPFFSANVIPAYQIVRPDW
                     ALALMPTCAMLHGIFELSGLQSWTWLVDLPMAASALYLTYAWRLRASLAVPILGMLHI
                     AFAWLGIALLLYAAQSLSLLMTGHFILAKAPLHALTIGYFTSMMLAMVTRVTLGHSGR
                     VLKVDRLTWDLFLAFQFVAILRVGSELPGIGLTSRSHFYLCAAVVWLACFSLWAWKFA
                     PIYWKPRADGAPG"
     gene            complement(16163..16921)
                     /locus_tag="PKW44_00880"
     CDS             complement(16163..16921)
                     /locus_tag="PKW44_00880"
                     /inference="COORDINATES: protein motif:HMM:NF016843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPP family protein"
                     /protein_id="HQR50173.1"
                     /translation="MFTAVIKKITRPLNIMKLTTPLGTCFAGYQSSHTSIREKIFASV
                     GSFAAILILVVVVHYFSVGTSFSLPVLASMGASAFLLFVVPHSPMAQPWALVGGHLVS
                     AAIGVACAQWIHNPVFATATAVAISIFAMHWMHCLHPPGAATAMIAVLGGPEVHAIGW
                     RFCYEVVAINAGMILLLAFIINNLIPGRRYPLSHSHHPHHAQFGKVDHKPYAELKEED
                     FKWALGQMDGVIDVSEEDLVDLYEFATVHAQHRK"
     gene            complement(16932..18062)
                     /locus_tag="PKW44_00885"
     CDS             complement(16932..18062)
                     /locus_tag="PKW44_00885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR50174.1"
                     /translation="MLPFQAAVTLLTFIAASFLALSRGSAAIVSYHIIFAIGIVPLIL
                     GAMVHFVPVLARSKSPGQCIRLLPATAMLGGIVVTSYFAFPQAVPAGRYLGAAIVIIA
                     VASTGYWAYRSRSKAIGTPHPCLDWYLAAMACLFVAMGAVLLGYFVPTQRAALRLLHL
                     HLNAMGFIGITALGTLQVLLPTVAQRPDPKATIRMHQHLKWVVAGTVMTVCGAAWHSG
                     LAWIGVTLLAIPLFGIFKSWLPLYAGEIFNMHGTTPALAAALCGYVVAVVLGTTHAYH
                     YTGASPVATFIIAFLMPLVTGAISYLLPLWLCPGQQTAWHRAARKYLGFGGGLRALIL
                     LAGGVMAGLGHGIGWYLAAFAASTFLAQTLLLFTLVRASPQA"
     gene            complement(18079..18336)
                     /locus_tag="PKW44_00890"
     CDS             complement(18079..18336)
                     /locus_tag="PKW44_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019898611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2249 domain-containing protein"
                     /protein_id="HQR50175.1"
                     /translation="MSQPHLHLVPEQIYPFDARGIAKRFRHAAIFGALDALNSGETMR
                     FVNDHDPLPLLNQLEQRYGNAVSIQYVQREPGHIVIDFVRT"
     gene            complement(18405..18800)
                     /locus_tag="PKW44_00895"
     CDS             complement(18405..18800)
                     /locus_tag="PKW44_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR50176.1"
                     /translation="MEPQILTAISQKRAAQLFNVLSMIAVILMPAFPILLIWGAGSIM
                     VYAASAHHPNPVVREYIRYGGYRFYGLAGSLLVMLTFSNELKKIFGGALHMWLAVWAI
                     SFLVIVPLGLRDVWRARKEEWRDMPLESM"
     gene            complement(18838..19926)
                     /locus_tag="PKW44_00900"
     CDS             complement(18838..19926)
                     /locus_tag="PKW44_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR50177.1"
                     /translation="MHHNLSVQYRIPMLMAGFVSLVFGIGTGLLRLGWNFPLPSAGLM
                     AFHGPLMVCGFLGTVISLERAVAIGRRWAYLGPLSAALGGVAFITGLPWLIGAALVTS
                     ASVILGIASAHVFFRQRALFTLTLLLGSVSWLLGNLLWLSGLGIAQVAPWWIGFLVLT
                     IAGERLELSRFLPPSPNSKITFMVIITLFLIGTVSATLSDAWKVQTLAAALLALALWL
                     LRQDVARHTIKQAGLTRFIAACLVSGYVWLLMGALVGLLSPHLMSGSSYDAFLHTILV
                     GFVFSMIFGHAPIIFPAVARVRIPYHPTFYLPLIALHVSLVARIAGDLLQNPQSRSLG
                     GALNAVALLLFVLSTISAVIRGKRQLVQ"
     gene            complement(19942..20196)
                     /locus_tag="PKW44_00905"
     CDS             complement(19942..20196)
                     /locus_tag="PKW44_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2249 domain-containing protein"
                     /protein_id="HQR50178.1"
                     /translation="MSNATIKTTVDVRTVPPPQRHPMIFSTFNQLAPGEAMMLVNDHD
                     PKPLFYQFQAELGDIFTWNYIEQGPEVWQVRIGRTAACCA"
     gene            complement(20232..20564)
                     /locus_tag="PKW44_00910"
     CDS             complement(20232..20564)
                     /locus_tag="PKW44_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019898667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HQR50179.1"
                     /translation="MSMKTCNEPLSPLEQSVRDALLRVIDPEIGENIVDLGLVYGIEV
                     EGNVVKVKLTMTSPACPMGEMLLDDIHASLTRMLPGDMEFRIHLVWVPPWDPSMISAE
                     AKQRMGWA"
BASE COUNT         4408 a         5919 c         6112 g         4361 t
ORIGIN      
        1 cgatgacttc gacgatttcc ggagtgagcg ttttgccgac gatgccgcgg atgtcgtagg
       61 ccttgaagat ttcctgaggt aaattcatga cacgcagttt tgcgaatgga ttcaaagccc
      121 agagtataat gccagctatg gaattctcat cagaactgct gcacaaatac tgcaaggccg
      181 gcccgcgcta cacctcgtat cccaccgcgc cttatttcca cgaaggcttc ggcccggcgc
      241 aatgggaaga agaactgcgt gagagccaga acaaggggcg cgacatttcg ctctacgccc
      301 acattccgtt ctgcgacacg ctgtgctact actgcggctg caacatgatc gccaccaagg
      361 actacagcaa ggccgagtcc tatctggaag tactgttcaa ggagatcgac cgcgtcgccg
      421 cgctgaccaa tccgcaacgc accgtgcgcc agctgcactg gggcggcggc accccgacct
      481 atctcaagcc ggacgacatc cgccgcctct atgcgcacat tgccggcaag ttccgcatcg
      541 cgccggacgc cgaaaccggc tgcgagatgg atccgcgcga actgacccag gagcacgtgc
      601 gcgccttgcg ccagtccggc ttcaaccgcg tcagcctggg cgtgcaggac ctcgacgccc
      661 aagtacagca cgccgtcaac cgcgtgcagc cggaagcgct gatccgccag gtctatgact
      721 ggatacgcga gcaaggcttc cacagcgtca acttcgacct gatgatcggc ttgccgcacc
      781 agaccccggc cagcttcgag cagacgctgg acaaggtgat cgcgatgggg ccggatcgct
      841 tcgccgtgtt caactatgcc cacgtgccgt ggatgaaaaa gcaccagaag ctgatcgccg
      901 aggaaaccct gccgcagatc gacgcgcgcc ttgcactgca aaagctcacg ctggagaaac
      961 tcaccgccgc cggctacgtc tacatcggca tggaccactt cgcccgtccc gacgacgagc
     1021 tggtcaaggc gcagcaggcc aagaccctgt accgcaactt ccagggctat accacgcaca
     1081 aggactgcga catcctggct ttcggcgcct cggccatttc ccagaccgat gaggtctacg
     1141 cgcagaacgt caaggtgctg tccgaatacc gcgcgatggt tcaggccggc aagctgccgg
     1201 tcgagcgcgg cttgaggatc acccatgagg acaagctgcg ccgcgaagcc atcacccaga
     1261 tcatgtgcga ccttgaactg gacaaggcca gcttcggccg gcagtgggac atcgatttcg
     1321 atacctactt cgccgcagcg ctggacgatc tcaagggcat gcaggccgac ggcctggtgg
     1381 cgctggaagc cgacaaggtg cgcgttaccg aggaggggcg cgtattcctg cgcaatatcg
     1441 ccatggcatt cgatgcttac ctgcaccagc aggcgtcgga caagccgaga tattcgaaga
     1501 cggtttgatt gggggaaagg aatgaccatg tggtctgaaa aacttgaatc cgcactgtcc
     1561 gggttcctcg tcatgatgac gcggcgagtg cgatcgatgg ccgcggcatg cggcagcctg
     1621 gtgttgtgca cgctgctgct gatgttggcc ggcaacgtct atgcggtaga agtgccaaag
     1681 gctgcaccgg atccgaccgc gctggagacg gatgaagtcg tgccgccggc cgacatcgtg
     1741 gtttacaacc gtgtgatcat aaccctgcgc gcgcccgctt tcagcgaatt gccggaagtg
     1801 cgcgcgcgct atgccgagac caggatcaag gaggtgctca agggcggggg gggagaccgg
     1861 gtcgaagtga aacgtattcc acaagggcag atggttcagc tcaatggccg gctggcgttc
     1921 atcatcactc ccggcgatgt cgacccgaat acgcaggaaa cgccaattca ggccgcagct
     1981 gaagctgtca agaaactcga aatcgtcatt gccgaaacca gggaaagccg cagccttgat
     2041 gcgatactga aagatgtggc gctggtgctg ctgggaaccg ccgttttcta tgcattgttg
     2101 cgtagtgtgc gctggctgcg ccgttatctt ctggggtggc tggcgcgtct ggcaagcacc
     2161 gaggccgagc gcgccaagat tggcggtgtc cagatattcc ggctgagggg ggtggttacc
     2221 gtgtttcggg gcatgctggc caccctgttc tggatgttta ccgcggttct gatctacgtc
     2281 tggctggcct atacgctgtc acgttttccc tatacgcggt attggggcga aaccctgaat
     2341 accttcatcc tcaaggtgct gaatggtatc ggtggcgcca tcctgggggc actgcccaat
     2401 ctgtttgtcg ctttagtgat tgtggtgctg gcgcgactgg tggtgcgaat gctgcacggg
     2461 tttttcgata tggtagagag ccggcagatc gagctgcgat ggatcgatgc cgatctcgcc
     2521 acgcctacgc gacggatcgc caacgtcatc gtctggttgt ttgccttggc catggcctat
     2581 ccctacctgc cggttgccaa tacggaagcg ttcaaggggg ttaccctgct gttcgggctg
     2641 atgctttcgc tgggggcttc cggtctggtc gggcaggcgg tgagtggcct gatcctgacc
     2701 tatgcgcggg tgttgcggcc gggtgaattt atacacatcg caggcaacga gggtaccgtc
     2761 acggagactg gcgtgttcgc cacacgcgtt cgcaccggca tgggcgaaga actcacgctg
     2821 ccgaacactt tcattctgtc caatgtcacc cgtaattatt cgcgtgcggt caaggggaaa
     2881 gggtatatcg tggatgccac ggtcactatc ggttacgaca ccccctggcg ccaggttcac
     2941 gccatgctga tcgaggctgc caggcgcacc ccgggggtgg tggaaaatcc actgccgcgc
     3001 gtgtaccaga ttgcactgtc ggatttttat accgaataca agctggcctg ccatgcgggt
     3061 gaggaggagc cggtgccgcg cgcggaagtg atgagtttgc tgcacgcgaa tatccaggac
     3121 gtgttcaacg agtatggcgt gcagatcatg tcgccgcatt actttgccga tccggcggcg
     3181 gccaaggtgg tgcccaagga gcattggcat ctggcgcctg cggaccccga cgcgccgatg
     3241 ggtaacattg aagttgtggg ggaaaagccg aaaagccggt aaccggaact agcggtggcc
     3301 ttccttggcc atgttgatag agtatttggg aatctccacc accaggtcgg tctgcgcgac
     3361 gatggcctgg cacgacaggc gtgaaaacgg ctccagcccc caggccttgt ccagcaggtc
     3421 ttcttccttt tcctcgggcg ctggcagcga cgtgtagccc tcgcgcacga tgacgtggca
     3481 ggtggtgcag gcgcaggctt tctcgcaggc gtggtcaatg tcgatgtcgt ggtcgagcag
     3541 gttgtcgcag atggaaacac cggccttcgc ctcgatcacg gcgccttccg ggcagagttc
     3601 ctgatggggc aggacgatga ttttcggcat aggtcggatt ctacagctta agttcgtcga
     3661 gtttgtggcc ggccagcgcc tgcttgaccg aagcgtccat gcgccgagcg gcaaaagttt
     3721 cgctggcgcg gttgagggcc tcgatgccgg cgctgatggc gcgggcgtca ttgttggccg
     3781 ccagtccgcg cagtgcagcc atctgctgtt ccagcgcagc gcgttccgta tcggttagca
     3841 gcgctccatc cttgcccagg gcggcggcaa tggcttccag caggcgctcg gcctccacct
     3901 gcgcctcacg cagggcgcgc gcctgcttgt cgtcctcggc gtgcgaaaag ccggattgca
     3961 gcatattggt gatctcgtcg tcactcaggc cataggacgg cttgacgact acgtgcgctt
     4021 ccacgccgct ggtttgctca cgcgcagaga ccgacagcag gccgtcggcg tccacctgga
     4081 aggtcacgcg gatgcgcgcc gcaccagcca ccatcggcgg aatgcctttt aattcgaagc
     4141 gggccagcga gcggcattcc gacaccagtt cgcgctcgcc ctgcaccacc tggatgctca
     4201 tggcggtttg gccgtccttg aaggtggtga attcctgcgc gcgcgccacg gggatggtgg
     4261 aattgcgcgg gatgattttc tcggtcaggc cgcccatggt ttccaggccg agcgacagcg
     4321 ggatcacgtc cagcagcagc aactcgttat cgttcttgtt gccggccagc acgtcggcct
     4381 gaatggcggc gcccagcgcc actactttgt ccgggtcgag gttggtcagt ggttcctgcc
     4441 ggaaaaattc cgccaccgcg cgctggatct gcggcatgcg cgtggcgccg cccaccatca
     4501 ccacgccttg cacctcctcg atgccgattt tcgcgtctcg cagtgccttg cgtgtaggcg
     4561 tcagcgtttt actcaccagg tattgggtca ggtcgttgaa gatctggctg gtcagggtga
     4621 ggtcgatcag gtcgccgctc gacagcatgc aggtgatgcg cgtgtcggcg tggtcggtca
     4681 gttgttcctt ggcctcgcgc gccttggtca gtagcaggcg ggtgtcctcc gggccgatgg
     4741 ggggaagctt ggcctgttcc agcacccagc agaacacgcg ccggtcaaaa tcatcgccac
     4801 ccagtgcgga atcgccattg gtagccagta cctcgaatac gccgcgcgac agccgcagca
     4861 cggacacgtc gaaggtgccg ccgccgagat catagaccac gaatacgcct tctgcagcat
     4921 tgtccaggcc gtaggccact gcggcggcgg tcggttcgtt gagcaggcgc agcacattga
     4981 gcccggacag ccgggcggca tccttggtgg cttggcgctg ggcgtcgtcg aaataggccg
     5041 gtacggtgat gacggcgcca gtgagttctc cgcccagcga cttctcggcg cgggctttca
     5101 gcaccctcag gatttcggcc gagatttcca ccgggctctt gctgccggcc acggtactaa
     5161 gctgcagcat gcccggtgca tcgacaaagc ggtaagggta atggccgaga tcggcgatgt
     5221 cgtgcacgcc ccgccccatg aagcgtttgg cggagacgat ggtgttgtgc gggtcgcggc
     5281 tttgctgggc ttgcgcggta tagcccactt cggcgccacc atcggccaga tagtgtacta
     5341 ccgagggcag cagggcgcgg ccctgctcgt cgtgcagcac cacgctcatg ccactgcgta
     5401 tggtggcgac cagcgagttg gtggtgccga ggtcgatgcc aatcgccagc cggtgctggt
     5461 ggggtgcggc gctttggccg ggttcggaaa tttgcagcag tgccatcagt cttccagcgc
     5521 ttcaatcgcc tgttcgattt cggtgcgcac tttatcaaga aagcgcagtt tgcgcacggt
     5581 ttcggcggcg ctggcgaaat ccttgtgctc gtccaacatg gcgtgcaggt gagactggtg
     5641 ggccgcagct gcattgtgga gttcgcgtag cagtcggtcg agcgcgggga tgttgtccgc
     5701 ctcgcgcgct tcctcgatgg cctctcgcca ctccatctgc tgcatcagga actcggctgg
     5761 catcgcggtg ttggtatttt cctgcgtgtc gatgccgttc atgtgcagca ggtagcgtgc
     5821 ccgggcaatg ggggatttca gcgtctgata ggcttcgttg acatgtgtgg ccagttgcat
     5881 cgagcgcaga cgctctgcgg gggtggcggc ggcaaagcgg tcggggtgaa cctcgccttg
     5941 cagcttgcgg tagctgtggt cgagatgacc cagttcaata gcgtaggaga ccgggaggcc
     6001 aaacagttcg aagtggttct gggtgaagtc gggggccacg tgggtatcca gaaaaacgcg
     6061 ggggagtcag gcggccagac cgggccgccg cgaggttaca aggtgaagct ctctccgcag
     6121 ccgcattcgg ctttgacgtt gggattgttg aacttgaagc cttcgttcag gccttccttg
     6181 gtgtaatcca gctccgtacc gtctagatag gccaggctct tggggtctat cagtatcttg
     6241 acgccgtggc tttcaaaagt caggtcttcc ggcaggattt catcggagaa ctccagcgta
     6301 taggccatgc cggagcagcc agtggtcttg acgcccaggc gcaggccaac ccctttgccg
     6361 cggttggaca ggaatttttc tacgcgcgct gccgcatttt cagtcagtgt gatcgccatg
     6421 cttacccctt gcaggctgca gtttcagtgc tggagccctg tttctgcctg agatcggcga
     6481 tggccgcctt gatggcgtct tccgccagca ccgagcagtg gatcttgact ggtggcaacg
     6541 ccagctcttc ggcgatgtcc gagttcttga tggacattgc ctcgtctagc gacttgccct
     6601 tgagccattc ggtcaccagc gatgaggatg cgatcgccga accgcagcca taggttttga
     6661 acttggcatc ctcgatgatg ccgttttctc ccatcttgat ctgcagtttc atcacgtcgc
     6721 cgcaggccgg ggcgccgacc atgccggtcc cgacgttctt ctcgtccttg ttgaaggaac
     6781 ccacattacg ggggttctcg tagtggtcca gtactttttc gctatatgcc atgatattgc
     6841 tccttgctca gtgggcagcc cactgtacag aattgatgtc gatgccgtcc tggtacattt
     6901 cccacaacgg cgaaagctcg cgcaacttgc cgatcttggc cttgagaagg tcgatggtgt
     6961 agtcgatttc ttcttcggtg gtatatcggc cgatggagaa gcggatcgaa ctgtgcgcaa
     7021 gttcgtcgga acgccccagt gcacgcagta cgtaggacgg ctccagactg gccgaggtgc
     7081 aggccgaacc cgaggacact gcgatatctt tcaccgccat gatcagcgat tccccctcga
     7141 cgaagttgaa gctgacattg aggttgtgcg ctacgcgctg cttgaggtcg ccgttcagat
     7201 agacttcttc gatctcggac agccccgccc acagtttgtc gcgcaggcgc ttgatgcggg
     7261 cgttttccag cgccatttct tccctggcga tgtggaaggc ctcgcccatg ccgacgatct
     7321 ggtgcgtcgg cagggtgccg gagcggaagc cgcgctcgtg gccgccgccg tgcatctggg
     7381 cctccaggcg gacgcgtggt ttgcggcgca catacagcgc gccgatgccc ttgggcccgt
     7441 aggtcttgtg cgccgagaag ctcatcaggt cgaccggcag cttttccagg tcgatctcga
     7501 ccttgccggt ggcctgcgcc gcgtcaacat ggaaaatggt gccgttggcg cggcagaggt
     7561 tgcccagggt ttcgatatcc tggattacac cgatctcgtt gttgaccagc atcaccgagg
     7621 ccagaacggt atctgctctg agcgcggcct tgaacttttc aaggtcaacc agcccgtcgg
     7681 gctccggttc gaggtaggtg gcttcgaagc cctggcgttc cagttcgcgc acagtatcga
     7741 tcactgcctt gtgttcggtg gcaatggtaa caatgtgctt gcctttggag ccggcataga
     7801 agttcgccgc acccttgatg gcgaggttgt tcgattcggt agcgcccgag gtccagacga
     7861 tttccttggg atcggcattg accagtaccg caacctgctc gcgggcattc tcgacagctt
     7921 tttccgccac ccagccgaac acgtggctac ggctggccgg attgccgaaa tgctcggtca
     7981 ggtaagggat catcttctcc gccacgcgct ggtctaccgg cgtggtagcg gaataatcca
     8041 ggtaaatcgg tgttctcaca gtcatgcttg cttcctcaaa ccgcaaccgc ggccattcgt
     8101 ttgattcgta acaattcgtt tttcaacgtt tgtaaaaacg ctactatctg ggctgcgctg
     8161 ttgtccaccc ccaggcttac ccgtacggcg ccgcgggcga tccccggtgc gatacccatg
     8221 gccatcaata catggctcgg ttcaatactg tcgctggagc aggccgagcc gcttgcgacg
     8281 gcgtagcctt gtctgtccag cgccatgacc agcgtttcgc catcgatgtt cgggaaagcg
     8341 aaaaaactgg tgttcggtag ccgtttggct tcccgcccga aaatgatgcc tcccagttcg
     8401 ttcagcccct gttccagttg atcgcgcagc ctgccgattc ttcctgcttg agaggtcagg
     8461 tcgcgggtcg ccagttcgca ggcgaggccg aagccgacga tagcagagat gttttccgtc
     8521 ccgctacgca agcctttttc ctgaccgcca ccatccagca gcggctggat gtccacgcgc
     8581 ttgtccagga tcaatgcgcc ggcgccttgc gggccatgaa tcttgtgggc cgatatgctc
     8641 atggcgttga cccccagcgc attgaagtcc agtgcaacct tacctagcgc ctgcaccgca
     8701 tcggtatgta gcagactgcc gtgccgtctg actacttcgg cgattgccgg gacgtcctgt
     8761 aatacgccgg tttcgttgtt ggccgccatc accgatacca gcgcggtttt ttgctgcaac
     8821 gcctgcacca gttggtccgg gttcaactgg ccgactgcat ctacgccgat tttcgccatt
     8881 ttccatccgc ggccgagtag cgagtgcgcc ggtctggaca ctgccgggtg ttcgatggcg
     8941 ctgacggcga cttgcgaggg tgtatgcgtt gccgcgatgc ccttgattgc caggttgttt
     9001 gcttcggttc cgccgctggt gaaggtgacc tgtgaaggat gtgcgccgac ggctgcggcg
     9061 acctgttctc gcgcatgatc gatcgcctgc cttgctgcac ggccatagct gtggcggctg
     9121 gtcggattgc cctgctggtt gcgcaggaat ggcagcatcg cctccagcac cttcgcatcc
     9181 agtaaggtgg tggcgttatg gtccaaatag acggcttcgg cgctcatctc gattctggcg
     9241 gtcgcaatca ggccgctatc ggttgcaact gtgcctcgcg gcgcttgggt tcctggatga
     9301 ggattttctt gccttcgcgt tgcgaggcga ccagttccgc cagactgaca ccggagagat
     9361 attccagaat cttgacgttc aggctggacc acaggtcatg cgtcatgcaa cgccgctcgc
     9421 catggcaatc ttcgctgccg ccgcattggg tcgcatcgat ctgttcgtca acggcccgga
     9481 tgatttcgga gactgaaata tcctcatgct ttttggcaat acggtagccg cctccgggcc
     9541 cacgcacact tttgaccagg ccgttgcggc gtagccggct gaacagctgt tcaaggtagg
     9601 acagcgaaat ttcctgacgt tcgctgatgc ctgagagtgt gacggggcgg tcaacttcat
     9661 tcagtgccag gtccagcata gccgttacgg cgaaacgtcc tttggtggtc aagcgcatgg
     9721 cgggtgctcc gttcgaattc aatcaaggtg aaatgtataa taccctaaca ttttagtcaa
     9781 ctatttgttg ggatggttca cgtcgaaagc cttgccgacc tcggcatcgg tttccgtgtc
     9841 cgcgcccagt ttggcgagca actgtgtcac ttcctccagt cgcttgtcct gctcggcggc
     9901 gtggtcgagc agggcgtgga tggccttgac catggggtcg ttcatgtcgt cggatacggc
     9961 gtaggccgag aagcccatct tttgcgccat gtcgttgcgc tgctggacct tgtcctgatc
    10021 caggatgcgc gccggaatgc cgatggccgt ggtccctgcc ggtacatcct tgaccactac
    10081 cgcgttggag ccgatcctgg cttccgtacc tacggtgatg ggccccagca ccttggcgcc
    10141 ggcaccgatc accacaccat tttccagagt ggggtggcgc ttgcccttgt tccacgaggt
    10201 accgcccaag gtgacgccgt gatacagggt gcagtcattt ccgatcaccg ccgtctctcc
    10261 gatgactacg cccatgccgt ggtcgatgaa cacgcgccga ccgatcgtgg cgccggggtg
    10321 gatttcgatg ccggtcagga agcgtccgag atgcgagctg aagcgcgcca gccagcgcag
    10381 gcgcaggctc catagccagt gcgaaaatcg atggatgatg agggcgtgta caccagggta
    10441 ggtcgtgagg acttcccaat gggtgcgagc ggccgggtcg cgttcgaaca cgacagcgat
    10501 gtcttcgcgc agatgattga acatggcaaa aatcttgact gaaacaagtg gttattttgc
    10561 cataaaaaca gcctgaccgt cacttgggcg attgcatgaa cttcaatatg ccacgcaaga
    10621 tattgacttc ctccttctcc agctgcgccc gtgcaaatag tctgcgcagg cgtggcatca
    10681 gccgtttcgg catggcgggg ttaaggaacc cgatgcgtaa cagggtttct tccaggtggc
    10741 gatggaagcc ctcgatttcg tcgtggctgg cagggggaaa gctggggtca gttgccgcag
    10801 gcgcgaaatc cgtcgccatg cgcagttcat aagtcagcac ttggaccgct gccgctagat
    10861 tgagcgagga gtactcgggg ttggcgggga tcatggccag tcgctggcac tggatcagct
    10921 cggcattcga caacccgctc atctcggtgc cgaacaccag tgcggcatgc tggctggagg
    10981 cggcttgagc tattaacgtg gcggcctcgt gtgctgtaat cagttcatgc gacagttcgc
    11041 gcttgcgcgc gctcaagccc accaccagca cgcagccggc cagcgcttcc tgcagatttt
    11101 cacacacccg cgcctgcgcc aagatgtcgg tggcgccgga ggccatggct tcggcttccg
    11161 ggtgggggaa tttcttcggg cgcaccagca ccagttggtg caatcccatg gtcttcatcg
    11221 cccgtgccgc cgcaccgata ttgcccggat ggctggtctg gcagagcacg atgcggatgt
    11281 tggcgagcga ttcaggttta ttcatagccg gcaaaacgct aaaatgccaa ttttaacagc
    11341 tctttaaagc ttccctcggc gggaagcaca ctgaaagccc atcatgcacc ccatgctcaa
    11401 tattgcggtg aaagccgccc gcaacgccag tgccattatc acccgtgcct cgcaggacct
    11461 gggtgcgctc aaggtgcgca gcaagaccta caacgactat gtcagcgaag tagaccacgc
    11521 cgccgagcgg gccatcatcg aaaccctgct cgacgcctat cccaaccacg ccatcctggc
    11581 cgaggaaagc ggcgccagtg gtagcaatgc cgagaatctg tggatcatcg atccgctcga
    11641 cggcaccacc aacttcctgc atggttttcc gcagtactgc atttccattg cgctcaagca
    11701 gcgcggccag atcacccagg cggtgatcta cgaccccaac cgcaacgacc ttttcacggc
    11761 cacgcgtggc agcggtgctt ttctcaatga tcgccgcatc cgcgtcagta cccgcaccag
    11821 gttgcaggag tcgttgatcg gtaccggcct cccgttccgc gacttcactc atctcgacgc
    11881 ctatcttgcc atgttcaggg acatgatcaa gaagacctcc ggtctacgcc gccccggctc
    11941 tgctgcgctt gacttggcct acgtcgccgc tggctggtat gacggcttct gggaaatcgg
    12001 cctgtcgcag tgggacatcg ccgccggcag tctcttggtg cacgaggccg gcggactggt
    12061 gggcgacttc gagggcaacg aaacctggct ggaaaccggc aacatcgtcg ccggcaaccc
    12121 caagctgttt gcccagatac tgcaagtgct ccatccccac ctgacggaag cgctcaagtc
    12181 tgccaatgct ggcgtttgac gccatcgccg agcagcgcat ctgtgaagcc gccgcgcgcg
    12241 gcgaactgga tggcctgccc ggcaccggcc ggccgctggc cctggatgac gacctgctga
    12301 tcccgcagga gctgcgcatg gcctaccgca tcctcaagaa tgccggctac gttccgcccg
    12361 aagtgcaaac gctcaaggag atcggcgaac tggagcgttt gatccagtca ctggacgatg
    12421 gtaagcagcg cagcctggca cagcgcaaac tgcgcctgct gaccctgcaa atgaacgaat
    12481 cccgctgcgg cagcctgcaa ctggaagccg cttactacga caagatcgtc agccaactgg
    12541 ccgcatgaac gaccccgccg gtttgcagca gcacacccca tgatgcgtca gtatctcggc
    12601 aaatataaaa taatgtatat aaaacaataa catcagatac ttttaattta tttcaggata
    12661 cttataggct acatttttgt ggcgctcgca aatcggggct gtggctcagt tgagaaagac
    12721 gttctgaact agcccagctc cgctcctttt agctatcgat atcgatctgt cgttgccgtc
    12781 ggagcgggtc atacgggccc tggatcagat catcgagtgg cgcggcaagc cgctggcgat
    12841 tcgcagcgat aacggtccgg agtacatcag cggcaccttc atcgcctagg ccgaacgtag
    12901 aggcatccgg cttgatcaca tccagcctgg caacccgcaa cagaatgcct acattgaacg
    12961 ctacaaccgg acggtccgct acgactggct aagccactac ctgttcgagt cgattgagga
    13021 ggttcagaac tatgcgaccg actggatgtg gacttacaat cacgaacgcc ccaacatggc
    13081 gcttggcggt atcaccccga aacagaagtc agctctttta accaactcta cttctgaccg
    13141 ctgctaacaa tggggggatt accggtaaac ctgtaaggca taaggagtat gcgatggctg
    13201 cctggaatag tgggaaagtg gttgaacacc gccagtggag ccaaacgcta tattcacttt
    13261 atgttgagtc tgatatcgag gcatttgaag ctgggcagtt cgtgaagata gctttggaaa
    13321 ttaatgggga ggtgataggg cgaccgtatt ccttggtaaa tccacccgat tgcaggccat
    13381 tggagttcta ctatattgaa gtgccaaatg ggccgctgac ttcccacctg atcaagctca
    13441 taccaggtga taatattctg gtcgctccac gtgcccacgg ctttatggtg cttgatgaag
    13501 tacctcaggc aaagcaactg tggctgatgg ccaccggcac gggcattggg ccttttcttt
    13561 cgattctgat gacagataag ccctggcagc gctacgagca ggtggtattg gtatatgcgg
    13621 tgcggacttt atccgagctc agctatcagg agcgcataac gcaaattttg acaaaacatt
    13681 ccggacaatt tggcttcatc ccgtttctca gccgcgaagc tttggatttc gcactacctg
    13741 gccgcatccc gcaggcaatt attgatggcc gacttgaagc gcgtgccggt ataaaaataa
    13801 gcgcagaaaa cagccagatt atgctttgcg gcaatcctca aatggtaaaa gacataacca
    13861 atatactgat tgaacgtggc ttgagaaaac accgccgctt tgagcctggt caaatcacat
    13921 ctgaaaatta ctggcaaggg taggagaaac aaccccctaa ccataatgtc atcatgaatc
    13981 agagttatgt atgtggcaat tccgatccag ttcgactagc tgtgttaaca actcagagtg
    14041 cgcttgtacg tgaagaatat agcgcggtac attgcgatca gtggattgtg acatgaatgg
    14101 ctcaccccaa aaacttagca gagcctctgt agctccttgc ggctgagtgt cagccaaaat
    14161 gaaagacaaa ttcttatcaa gagagcacca acgtggctct tttatcttgc ttgcggcaat
    14221 gctattggcg attttcggta gaaccactgt ctgccacgtt ccctgctggc tgatgtccag
    14281 gacgaattga acgctggtta cctgggggtt ctcgatcacc ggacgcaata gtgcatcgaa
    14341 cagtggttga gttcggtaca ttgacaggca aacgttgaac agggttgcct cgcctcgcat
    14401 gttacgtgcg aattgctcgt tcgcggtgcg aagctgacgt ggaccaatga gtacgatatc
    14461 tgggactgcc aacgacgaat tgattttcgc caccattttg tggatatgtt ccacctgctc
    14521 tgctgtatgg tcattgcttc gtgtgtgacg catgaaattt atgaacaaga gcgccatgag
    14581 ggtgagcacg acaggcagga tgatttgttc gtcgatgaca tgcaaaaaat gcagcacgat
    14641 cgccaccatg gcagccagaa tccctgcgat ggcatcccat tcgtagctaa gcaaacggct
    14701 tcgcttcatg caatcctccg agttctgcag gcatgactgc gctgttctaa tgttgaaata
    14761 ctacaccccg aaatattttg ctgcaggatg atgtaccaca atcgggctgc tagattcttc
    14821 aggccaaagt tggtcttcaa ctgcagtgct tgaccggggt gtgctcgatg gctttgactc
    14881 cccagaaaag tggtgccagg atagaattat catggtaaac gagatacggg ttcatatcag
    14941 cccggcgctc catctgctct tggtttccag tagatggggg caaatttcca cgcccagaga
    15001 ctaaaacatg caagccaaac cactgcggcg cagagataaa aatggctgcg tgacgtgaga
    15061 ccaattccgg gcagctcaga ccccacacgc aatatggcca caaactggaa ggcaagaaag
    15121 aggtcccagg tcaggcggtc gaccttcagc accctgccgg aatggccaag cgtcacacgt
    15181 gtcaccatag ccagcatcat ggaggtgaaa tagccgatcg tcagggcatg cagcggggct
    15241 ttggcaagga tgaagtggcc cgtcatcagt aaagacaggc tttgcgcggc ataaagcagc
    15301 aaggcaatcc caagccaggc aaaggctatg tgcagcatcc ccagaatggg cacggcaagg
    15361 cttgctcgca gccgccatgc ataggtcaaa tagagtgcgc ttgccgccat gggcaaatcg
    15421 acaagccatg tccacgactg caaaccgctc aattcaaata ttccatgcaa catggcgcag
    15481 gtgggcatga gcgcaagcgc ccagtccggc cgtacgattt gataagcggg gatgacgttg
    15541 gccgagaaaa atggaatcat gcggtggctg actgcgaaga ataccggcag cagcagtagc
    15601 cagataccgc ccaccttggc gagcgctaca aaatgcgttt catcactcac aaatccggcc
    15661 accagcagcc acccgagcgc tagcacaacc gaggtgatcg cggcatgccg cttgtccggg
    15721 tgcttggcac gcagatagac gcgtagcagg gcagttagcc cggtgcccca ccccgccagg
    15781 aacagcacca gcgctatttt caacaaggtg aaactgagaa tcagccccac ataaaacagc
    15841 agcgtcccta tggcaagcag cagaaaagcc ggtacatata cgcggcgctc gacttcctcg
    15901 ccattcatcc agcgcgggta ggtagtcatc agaaagccga agatgaagaa cgggaagaag
    15961 ccgtagatca tcaaaaaggc gtgggaatcg atgggcggga gggcccatgc aatcggcgca
    16021 taccacccgc cgtagcgacc tcccaggtcg atcagccacc aactcaacgt aacgagcgcc
    16081 tgcaccgcgc cgccgaagaa aaacatgcgg tgtggcgcag atgtgtaggt cttccaagca
    16141 tcagtcaaat tcatgatgat tactactttc tatgttgagc atgtacggtc gcaaactcat
    16201 acaggtctac aaggtcttct tcgctgacat cgatgacgcc atccatctgg ccaagagccc
    16261 atttgaaatc ttcctctttc agttcggcat agggtttgtg atccactttt ccgaactggg
    16321 cgtgatgagg atgatgcgag tgactcagcg ggtaacgcct acctgggatc aggttgttga
    16381 tgataaacgc taacagcaga atcatacccg cgttgatggc gacaacctca tagcaaaagc
    16441 gccacccgat tgcatggact tccggaccac ctaatactgc gatcattgcc gttgccgcac
    16501 cgggcggatg caggcaatgc atccagtgca tggcgaatat cgatatggct actgcagttg
    16561 cggttgcaaa cacagggttg tgtatccact gagcgcaggc caccccgatc gccgcagata
    16621 caagatgtcc ccctaccaaa gcccaaggct gtgccatcgg gctatgcggc acgacaaaca
    16681 gcaaaaatgc tgaagcgccc attgatgcca agactggaag cgagaaggag gtgccaacgg
    16741 aaaagtagtg cactaccact accagaatca agatggcagc aaacgatccc actgatgcaa
    16801 aaatcttttc tctgatagat gtgtgggacg actggtagcc ggcaaagcaa gtacctaaag
    16861 gcgtcgttag cttcatgatg tttaaaggtc tggttatttt cttgatgact gcggtgaaca
    16921 atggcgggtt gctatgcttg aggcgatgct cgaaccagag tgaacaatag aagtgtttgt
    16981 gcaaggaagg tgcttgcagc aaaggccgcc agataccaac caataccatg ccccagaccg
    17041 gccatcaccc cacccgccag aagaatcaag gcgcgcaagc caccgccaaa tcccaaatac
    17101 tttctggcgg cccgatgcca ggctgtttgc tgacctggac acagccacag cggcagcaaa
    17161 tagctgatcg cacccgtcac cagcggcatg agaaaagcaa tgatgaaggt cgccacaggg
    17221 ctggcaccag tgtaatgata agcatgggtt gtgcccagca cgacggcaac cacgtatccg
    17281 catagcgctg ccgccagcgc cggcgtggtc ccatgcatgt tgaaaatttc tccggcataa
    17341 agtggtagcc acgacttaaa tatgccgaat aatggaatcg ccagcagcgt taccccgatc
    17401 caggcaaggc cggagtgcca tgcagcaccg cacacagtca tgacggttcc ggcaaccacc
    17461 catttcaggt gctggtgcat acgtatagtc gccttggggt caggtcgttg ggcgactgtg
    17521 ggcagcaaca cttgcagcgt gcccagcgca gtaatgccga tgaatcccat ggcgttgagg
    17581 tgtaggtgca acagacgcaa tgccgcgcgc tgagttggaa caaaataacc cagcagaacg
    17641 gcacccattg ccacgaacag acatgccatg gccgccagat accagtcgag acagggatgc
    17701 ggcgtaccaa tcgccttact tcttgagcgg tatgcccaat aacctgtgct ggcgactgcg
    17761 atgatgacaa tggcggcacc gagatagcgg ccggcaggta cggcttgcgg aaatgcaaaa
    17821 tagctggtga ccacgattcc accgagcatt gcggtggcag gtagcagacg aatgcattga
    17881 ccggggcttt tgctgcgcgc cagcaccggc acgaaatgca ccatcgcccc gaggatcagg
    17941 ggcacgatcc caatggcgaa aatgatgtgg tagctgacga tcgctgcact tccgcgcgat
    18001 aaggcgagga aggatgcagc gatgaatgtc agcagggtga ctgcggcttg aaagggcagc
    18061 acggctaatg cctgcaggtt aggtgcgaac gaagtcgatc acgatgtgcc cgggctcgcg
    18121 ctgcacatac tgaatgctga cagcgttgcc ataacgttgt tcaagctgat tcagtagcgg
    18181 cagcgggtca tgatcgttga cgaagcgcat ggtctcgcca ctgttcagcg catcgagcgc
    18241 accgaagatg gccgcatggc ggaaacgctt ggcaatgcca cgtgcatcga acggatagat
    18301 ctgttctgga actagatgga gatggggttg tgacatgggg tttcctttca cggaatgggc
    18361 gcgatatttg cgcgggtgtt gataaaaatg atgctgagtg tgaattacat ggattccagt
    18421 ggcatgtccc tccattcttc ttttctggca cgccacacat cgcgcaatcc caatggcacg
    18481 atcaccagga aactgatggc ccagacggcc agccacatat gcaaggcacc accgaatatt
    18541 ttcttcagtt cattgctaaa ggtgagcatc accaggagtg agcctgccaa cccatagaag
    18601 cgatagccgc cgtaacggat gtattccctg acgacagggt tgggatgatg ggcgctggcg
    18661 gcgtatacca tgatcgaacc agcaccccag atcagcagta tcgggaaggc tggcatcaag
    18721 atgacggcga tcatcgatag cacgttgaaa agttgcgcag cgcgcttctg ggaaatagcg
    18781 gtcagtatct gaggttccat gaattgggat tcctaaagca aatgtgaagt gatgttgcta
    18841 ttgcaccaat tgccgtttgc cacgaatcac ggcgctgatc gtgctcaaca cgaacaacag
    18901 cagcgcgact gcattcagcg ctccgcccag gctgcggctt tgcgggttct gcagcaggtc
    18961 gccggcaatc cgggctacca gcgagacatg cagagcgata agcggcaggt agaacgtcgg
    19021 gtgataggga attctgaccc tggctaccgc cggaaagatg atgggcgcat gaccgaagat
    19081 catggagaac acgaaaccga ccaggatggt atgcagaaaa gcgtcgtaac tggatcccga
    19141 catcaggtgt ggtgatagca atccgaccag ggcacccatc agaagccaga cataccccga
    19201 caccaggcat gccgcaatga aacgggttaa tcccgcttgt ttgatggtat ggcgtgccac
    19261 gtcctggcgc agcagccaga gggcaagcgc aagcaaagca gcagcaagcg tttgtacctt
    19321 ccaggcgtcc gacagcgtgg ctgagactgt gccgatcaaa aacagggtaa tgatcaccat
    19381 aaaggtgatc ttgctgttgg gagatggcgg gagaaatcgt gacaactcaa gccgttcacc
    19441 ggcgatcgtc aacaccagaa agcctatcca ccacggggcc acttgggcga tgccgagacc
    19501 gctcagccac aatagattgc cgagcaacca tgacacgctg ccgagcaaca gcgtcagcgt
    19561 gaacagcgcg cgctggcgga aaaaaacatg tgccgaggca atgccaagaa tgacactcgc
    19621 gctagtcacc aaagcagccc cgatgagcca gggcaggccg gtgatgaacg ccaccccgcc
    19681 cagcgctgcc gagagtggcc ccaaataagc ccagcgtcgg ccgatggcga cggcacgttc
    19741 caaagatatg acggtgccca ggaagccgca taccatgagc gggccatgga aagccatcag
    19801 cccggcagat ggcaatggaa aattccagcc taaacgcaac aatccagtgc cgatgccaaa
    19861 caccaggctg acgaaacctg ccatcagcat cgggatgcgg tattgcactg aaagattgtg
    19921 gtgcatgggg cgtgttttta atcaggcaca gcaggcggcg gttcggccga tgcgcacctg
    19981 ccacacttcc ggaccctgct cgatgtagtt ccaggtgaag atgtccccca gttccgcctg
    20041 gaactggtag aacagaggct tggggtcatg gtcattgacc agcatcatgg cttcaccagg
    20101 agccaactgg ttaaaagtgc tgaaaatcat ggggtgacgt tgaggtgggg gcaccgtgcg
    20161 tacatctacg gtcgttttaa tggttgcatt actcatagca acttctcctc ataacagtca
    20221 gaaaataagt gttaagccca gcccatacgc tgctttgctt cggcagagat catgctcggg
    20281 tcccatggcg gcacccagac tagatggatg cggaactcca tgtcgcctgg gagcatccgg
    20341 gttaagctgg catggatgtc atccagcagc atctcgccca tcgggcatgc cggcgacgtc
    20401 atggtgagct tcactttcac gacatttccc tcgacctcga tgccgtacac caatccgagg
    20461 tccacgatgt tttcgccgat ttccggatca atcactcgca acaaggcatc gcgaactgac
    20521 tgttccagcg gcgaaagtgg ttcgttgcaa gttttcatgg acattgatta agatgcatgt
    20581 aaaatgtatc ttaaatgtag cacagcgtct ttattaaagc aatattataa atacatctta
    20641 tatgggctga tgttatgcac ctcaccacct tctccgatta ctccatgcgg gtcatgatgt
    20701 acctgggtct ccaacatggt cagttggtga ccatttcaga tatcgcaatg gcctataaga
    20761 attccgaaaa ctactgaacg aaggtgatgc accacctggc
//
LOCUS       DAOMFA010000008        18775 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_200_length_18775_cov_5.463141, whole genome shotgun sequence.
ACCESSION   DAOMFA010000008 DAOMFA010000000
VERSION     DAOMFA010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 18775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18775
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_200_length_18775_cov_5.463141"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            179..1573
                     /gene="gltX"
                     /locus_tag="PKW44_00915"
     CDS             179..1573
                     /gene="gltX"
                     /locus_tag="PKW44_00915"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020181677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HQR50180.1"
                     /translation="MTVRTRFAPSPTGFLHIGGARTALFSWAYARKHNGKFILRVEDT
                     DVARSTPEAVQAILDGMSWLGLNWDEGPFYQMQRIYRYKEVIQQMLDAGTAYHCYTSP
                     DELTAMREAQMAAGVKPRYDGTWRPEPGKSLPTPPAGVPPVVRFRNPQSGVVAWDDQV
                     KGRIAISNEELDDFIIARADGTPTYNFCVVVDDWDMGITHVIRGDDHVNNTPRQINLL
                     HALGATVPRYAHLSMILGDDGQKLSKRHGAVSVTQYDDDGYLPEAVLNYLARLGWSHG
                     DDEVFDMAQFCQWFDLDHITPSAAQFNTEKLNWLNAHYLKQADLPRIAGEVSGRLAAR
                     GIAIEGGTDMQKAVELYRERASTLNELADAVEYFYQRIHPTQELLDKHLTPEILPVLQ
                     TLATQLASLEWAVESLHSAIEHAVVSNGLKFPKVAMPLRVLLTGGAQSPSIDAVMALL
                     GKEEGLRRLNEYLA"
     gene            complement(1584..2855)
                     /gene="ispG"
                     /locus_tag="PKW44_00920"
                     /gene_synonym="gcpE"
     CDS             complement(1584..2855)
                     /gene="ispG"
                     /locus_tag="PKW44_00920"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HQR50181.1"
                     /translation="MVQSRLDRRGTRRVLVGDIALGGDASVVVQSMTNTDTADAEATV
                     KQVYELWQAGSELVRITVNSPEAAARVGAIRKQLDALGCDVPLIGDFHFNGHKLLSAY
                     PECAEALAKYRINPGNVGKGSKRDEQFAAMIGMAIRYDKPVRIGVNWGSLDQAKMARM
                     MDENAQRAEPLSSEALMREALIQSALESAQQAEQWGLAGDRIIISCKVSDVQDLILVY
                     RELAHRCDYPLHLGLTEAGMGSKGIVASTAALSVLLQEGIGDTIRVSLTPQPGESRTT
                     EVVVAQEILQTMGIRSFTPLVTACPGCGRTTSTFFQELAQKIQRYLREQMPVWRSQYP
                     GVEGMSVAVMGCVVNGPGESKLANIGISLPGTGEVPVAPVFVDGEKTVTLKGNAIAEE
                     FQAIVEDYVRANYAQGGKLRREHKVIPLTPV"
     gene            complement(2868..3752)
                     /locus_tag="PKW44_00925"
     CDS             complement(2868..3752)
                     /locus_tag="PKW44_00925"
                     /inference="COORDINATES: protein motif:HMM:NF024855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4115 domain-containing protein"
                     /protein_id="HQR50182.1"
                     /translation="MSESTQEGGVMTDDGTGPVGAGALLTAAREQCGMSIEDAAGKLR
                     LSRNQLLALEAGDTASLPDVTFVRGFIRNYARLLQIDPEPLLLAYRSAMPSAHHSIAL
                     QSENIVIIGRGRRDWLPYVLGALLLLLALAWWMVYMDYRENAPAQQASAPASRQQEET
                     SPAAVPLADIPEPTPVPELEATSPPSPTPVPALVEEPPATQTAVVAPSVPAVPAKTLA
                     RLDVVFSDQVWMSVVDGAGKVIYENTGFSGDTKLIEGVPPLKLVVGNVEGVQMTFNGK
                     VVDLAPHARANVARFTLE"
     gene            complement(3749..4513)
                     /gene="pilW"
                     /locus_tag="PKW44_00930"
     CDS             complement(3749..4513)
                     /gene="pilW"
                     /locus_tag="PKW44_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis/stability protein PilW"
                     /protein_id="HQR50183.1"
                     /translation="MKKPLLLLWMVVALAGCVTERVNPYNVSKQETSARNRAKVHTEL
                     ASGYYARGMQAVALEEFSEAISIDPEYAPAYGGLGLVYTALNEEAKAESNYRKSLQLD
                     PTNSDMHNNYGAFLCARNRIDESISEFKLALKNPLYDTPEMAYLNAARCVLKKGDDSA
                     AETYLLRALELRPGMCQASYQLAQIAYGRAEYARAREEFNQSTRCAEPTAEMLWLGVR
                     IERALGDAGAEASYGMLLKNKYPKSEQAKAYLSGQK"
     gene            complement(4510..5595)
                     /gene="rlmN"
                     /locus_tag="PKW44_00935"
     CDS             complement(4510..5595)
                     /gene="rlmN"
                     /locus_tag="PKW44_00935"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HQR50184.1"
                     /translation="MTVNLLDFDQPRLAEFFAELGEKPFRAKQMMRWMHHFGVNDFAQ
                     MTDVAKSLREKLAQVAEIRPPAVKLEQISEDGTRKWLIDVGAGNGVESVFIPEADRGT
                     LCVSSQVGCALECTFCSTGRQGFNRNLDVSEIIGQLWLANKALGRDPKGARVISNVVM
                     MGMGEPLANFDNVVTALNIMLDDSAYGLSRRRVTVSTSGLVPAMDRLRDACPVALAVS
                     LHAPNDALRDQIVPINRKYPLAELMAACRRYLEKAPRDFVTFEYVMLDGVNDTVEHAR
                     QLLNLVKDVPCKFNLIPFNPFPDSGYETSRPENIRRFRDVLMQAGYVVTTRKTRGEDI
                     DAACGQLAGKVKDKTRRTHKLVLEAAA"
     gene            complement(5602..6009)
                     /gene="ndk"
                     /locus_tag="PKW44_00940"
     CDS             complement(5602..6009)
                     /gene="ndk"
                     /locus_tag="PKW44_00940"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HQR50185.1"
                     /translation="MAVERTLSIIKPDAVAKSVIGKIYSRFETNGLKIVAARMAQLSQ
                     AEAEGFYAVHRERPFFKDLVKFMISGPVMIQVLEGENAVAKNRELMGATNPKEAAAGT
                     IRADFAESIDANAVHGSDSAENAAIEIAYFFGK"
     gene            complement(6098..6592)
                     /locus_tag="PKW44_00945"
     CDS             complement(6098..6592)
                     /locus_tag="PKW44_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR50186.1"
                     /translation="MDTFSHALWGYGLFGQRSHPWLAMLFGALPDLVSFGALMVVRIW
                     DGSYHFGPPLLHTLPHWLFVSYDLWHSFVISLPVVGLVALFRRDIAFAMLAWPFHIVL
                     DFPFHSLRYFPTPIFWPLSDFTIDGVPWATPEVWLPNVAGLAILLAYRYRQKCRKQDV
                     PRQA"
     gene            6713..7861
                     /locus_tag="PKW44_00950"
     CDS             6713..7861
                     /locus_tag="PKW44_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HQR50187.1"
                     /translation="MRRILIFLILLAAAASAFWWFTRPKPIPVVLVEVGRGTVEASVS
                     NTRAGTVEACQHTRLSPITGGRIAYMGVKKGDHVKKGQVLIRLWNDDQQAQSGLAQTQ
                     VESARKRVGEICAMADSAEREAARMTKLRVKGFISEGGEEKARYEAQSRRAACEAMSA
                     DVTQAQARVTVTRVEQGRTVLVAPFDGTVADIVGELGEYTTPSPPGVATPPAIDLIDD
                     SCLYIEAPMDEVDAPKIRPGQPARVTLDALPGKVLPAHVKRVAPYVVAVEKQARTVDV
                     EANLDQPPPPGELLVGYSADLEVILDSRDNVLRVPTSALLEGDKVLLYQAASRKLEER
                     SLKTGIANWEYTEVLEGLNAGDRIVTSLERDGVKAGALARLEENPPAK"
     gene            7880..8557
                     /locus_tag="PKW44_00955"
     CDS             7880..8557
                     /locus_tag="PKW44_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQR50188.1"
                     /translation="MIELSGIARTFQLGDSKVHALRGINLGIAPGEYAAVMGPSGSGK
                     STLLNLLGLLDRPDAGTYFLEGRDVTTLSPDEQARVRSERIGFVFQSFHLVPRLTAAE
                     NIALPMMLAGIATAERGRRVAQALKDYGLADRANHRPDQLSGGQRQRVAIARATVMRP
                     AMLLADEPTGNLDRATGEEVMRLLEALNSQGVTLIVVTHDQTLGARAHRQIAMLDGAI
                     ASDTATS"
     gene            8554..9762
                     /locus_tag="PKW44_00960"
     CDS             8554..9762
                     /locus_tag="PKW44_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQR50189.1"
                     /translation="MNVADTLRYARAAASGTPLRTGLLILAMSIGVAAVVMLTALGDG
                     ARRYVVEEFSAIGSNLVIVLPGRSETRGFNPGNLVTSTVRDLTVEDAESLLRVPGVVR
                     VAPVIIGTTEINARGKLRETMLVGSSSDFIKVRHLKLAQGRFLSHDALGHGSAEVVLG
                     ALISSELFGSENPLGKTVRIGDRRLRVVGVLTEGGRGMGMTTNELLIVPVATAQAMFN
                     SNTLFRILVETRSRATVESTKAAVLKTIIARHDDEEDVTVISQDAVLQTFDKILGVLT
                     LGVAGIAAISLAVAGILVMNVMLVSVTQRTAEIGLLKALGATARAIRAAFLTEAAILS
                     LVGAVIGVVLGEAAAAVLRHLYPTFPAYPPPWAIVAGVGTALVTGLVFGIMPARKAAQ
                     MDPVQALSKR"
     gene            9899..10360
                     /locus_tag="PKW44_00965"
     CDS             9899..10360
                     /locus_tag="PKW44_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dps family protein"
                     /protein_id="HQR50190.1"
                     /translation="MDIGINAKDRKKIAEGLSRVLADTYTVYLKTHNFHWNVTGPMFQ
                     TLHLMFMTQYNELWLATDLIAERIRALGFPAPGTYAEFGKLTSIKDTTGVPKAEDMIR
                     LLVEGNEAVARTAREVFPVVEKASDQPTADLLTQRMQVHEKSAWMLRSLLE"
     gene            10496..11449
                     /gene="trxB"
                     /locus_tag="PKW44_00970"
     CDS             10496..11449
                     /gene="trxB"
                     /locus_tag="PKW44_00970"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018411282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HQR50191.1"
                     /translation="MSPKHARLLILGSGPAGYSAAVYAARANLKPVLITGMAQGGQLM
                     TTTEVDNWPADVNGVQGPELMARFQQHAERFNTEMIFDHIHTAKLTEKPIRLIGDAGE
                     YTCDALIIATGASAKYLGIPSEEKFAGKGVSACATCDGFFYRNQEVAVIGGGNTAVEE
                     ALYLANIASKVTLVHRRDKFKAEAILVDKLMERVKEGKIVLELFSTLDEVLGDDSGVT
                     GMRIKSVDTGATKDIALQGVFIAIGHKPNTDIFEGQLEMEGGYIVTQSGRIGNATATS
                     IPGVFAAGDVQDHIYRQAVTSAGTGCMAALDAERYLDHLGH"
     gene            11453..12013
                     /locus_tag="PKW44_00975"
     CDS             11453..12013
                     /locus_tag="PKW44_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HQR50192.1"
                     /translation="MKRPATPPSTIDEDEAGLFQEAVKGVRPLKQPRAHLPTPKPRPT
                     PQQLFRDECQALADSLSDGFIPAHEMESGEELLYLRDGHSPDILRKLRRGHWVVQGQL
                     DLHGMVVEEARAHVAEFLTHCKKRGTRCVRIIHGKGLGSKNREPVLKNKLRSWLMQRD
                     EVIAYCQARAVDGGSGAVVVLLKAGS"
     gene            12184..12672
                     /locus_tag="PKW44_00980"
     CDS             12184..12672
                     /locus_tag="PKW44_00980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR50193.1"
                     /translation="MRNKMILLGLVYAAFCGLLPIASHAEDAAMGEPVTVDIPPGTLP
                     TATLEVEATQIRLLIGGAKGKGVLHFNGTNYPFTLSAGSIGGVGYAEVKATGEVYFLE
                     KPEDLNGDYSAATAGVTAVKGAGMSTFKNAGNKVKLSIKSESEGLGLFLGIGAMKLEL
                     DK"
     gene            complement(12720..13199)
                     /gene="ispF"
                     /locus_tag="PKW44_00985"
     CDS             complement(12720..13199)
                     /gene="ispF"
                     /locus_tag="PKW44_00985"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HQR50194.1"
                     /translation="MIRIGHGFDVHPLVAGRKCIIGGVDIPHDKGLDGHSDADVLLHA
                     ICDALLGAAALGDIGKHFPNTDPRFKGIDSRQLLRHVSALLKEKGYGVINVDATVIAE
                     APKLAPHIIAMCANIAADLGLAADCVNVKATTSERLGFTGRGEGIAAEAVCLIESGR"
     gene            complement(13196..13885)
                     /gene="ispD"
                     /locus_tag="PKW44_00990"
     CDS             complement(13196..13885)
                     /gene="ispD"
                     /locus_tag="PKW44_00990"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HQR50195.1"
                     /translation="MPKNYVLIPAAGSGSRMGSELPKQYLPLHGKPLIHHTLSVFCAH
                     PAITGIFVVLSPEDTAWPGYGNDRVTVLRCGGETRAQTVLNGLDVIAGEVSGEDWVLV
                     HDAARPCLSPELLDRLLQMLQGDPVGGLLALPVADTIKREDAGGRVQCTEPREGLWRA
                     QTPQMFRYALLRDALRAMGTGIPTDEAQAVEFLGHAPKLVLGDGHNLKVTYPEDLVMA
                     GLILDNAGKNT"
     gene            13963..14763
                     /gene="amrB"
                     /locus_tag="PKW44_00995"
     CDS             13963..14763
                     /gene="amrB"
                     /locus_tag="PKW44_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HQR50196.1"
                     /translation="MQSIRPAAVAGMFYPGNPVTLAHDIDAMLVEAEARAPRLAPKVL
                     IAPHAGYIYSGPVAASAYALLKPVAERIRRVVLLGPTHRVAVHGLALPGAEAFATPLG
                     TVEIDLEAASLIAALPQVVTSPQAHAQEHSLEVHLPFLQSLLPDFKLLPLAVGMASAE
                     EVAEVLEILWGGDETLIVISSDLSHHLPYATAKRVDGATAQAIMQNQPVSHEQACGGT
                     PVNGLLLAARHHHLTPYLLDLRNSGDTAGSKDRVVGYGAFAFAGEARQ"
     gene            14760..15311
                     /gene="amrA"
                     /locus_tag="PKW44_01000"
     CDS             14760..15311
                     /gene="amrA"
                     /locus_tag="PKW44_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HQR50197.1"
                     /translation="MTEQEHGATLIRLARGAIAECFGLTVPPVPTDEDWLAVPGATFV
                     TLTEDGNLRGCIGSLAAQRPLVDDVRANAAAAAFHDPRFEPLRPEELDRTAIEISLLS
                     PTQTMQFADERDALAQLRPGIDGIVLEYQHHRGTFLPQVWEQLPIPEAFMTQLKRKAG
                     LPGNFWAPEVKLARYTVQKWKEN"
     gene            15314..16414
                     /gene="amrS"
                     /locus_tag="PKW44_01005"
     CDS             15314..16414
                     /gene="amrS"
                     /locus_tag="PKW44_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HQR50198.1"
                     /translation="MDEAIHIDERYPAQFWHRLDDGRIQCDLCPRDCKLHEGQRGACF
                     VRGAADGQMVLTTYGRSSGFCIDPIEKKPLNHFYPGSSVFSFGTAGCNLACKFCQNWD
                     ISKSRDMDRLMDQASPEAIAQAAKAHGSKSVAFTYNDPVIFYEYALDTADACHALGIE
                     TVAVTAGYIHAAPRKEFFAKMDAANIDLKGFTEDFYLKLTGSHLQPVLDTLAYVHHDT
                     GCWLEITTLLIPGHNDSDAEITALCKWVAQELGPEVPLHFSAFHPDWKMSDVPATPPA
                     TLSRARRIALDAGLHYVYTGNVHDTEGGTTFCPSCRHAVIVRDWYDIRHYDLTPQGAC
                     PRCGTAVAGRFGHYSKPFGSKRIPVRIATDLN"
     gene            16528..16740
                     /locus_tag="PKW44_01010"
     CDS             16528..16740
                     /locus_tag="PKW44_01010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR50199.1"
                     /translation="MTNLAEALNKELAEYEATLALSNGESLRIKGDTTSISDQFISVS
                     LSNNPDKKGRKLINIDHIVSIDWYTR"
     gene            16784..17257
                     /locus_tag="PKW44_01015"
     CDS             16784..17257
                     /locus_tag="PKW44_01015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR50200.1"
                     /translation="MRKKLINDPFQESEVADHDWLQVHDLAGVELTSEDAAHPIESVF
                     QVATASGWRAAEPGSQTIRLLFDQPQHLRLIHLDFVEPAVERTQEYVLRWSPDNGQTF
                     YEIVRQQWNFSPSGTRSETENHAVDLANVTMLELTINPDIGNRNATASLQRWQIA"
     gene            complement(17273..17530)
                     /gene="infA"
                     /locus_tag="PKW44_01020"
     CDS             complement(17273..17530)
                     /gene="infA"
                     /locus_tag="PKW44_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018412436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HQR50201.1"
                     /translation="MAKEELIEMNGVVMEVLPDSRYRVTLDNGHQLVAYTAGKMRKHH
                     IRILAGDKITLEMSPYDVSKGRITFRHIEGRGPAAAPRRRY"
     gene            complement(17630..17836)
                     /locus_tag="PKW44_01025"
     CDS             complement(17630..17836)
                     /locus_tag="PKW44_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HQR50202.1"
                     /translation="MATGTVKWFNDAKGFGFITPDGGGADLFVHFSAIQAQGFKTLQE
                     NQRVSFDSTTGPKGPQASNIRAAE"
     gene            18090..>18775
                     /locus_tag="PKW44_01030"
     CDS             18090..>18775
                     /locus_tag="PKW44_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HQR50203.1"
                     /translation="MAGQPEISNMALFCDFENIALGVRDARYAQFDIKKVLERLLLKG
                     SIVVKKAYCDWDRYKEFKSTMHEAAFELIEIPHVRQSGKNSADIRMVVDALDLCYTKA
                     HVDAFVIISGDSDFSPLVSKLRENNKYVIGIGVRNSTSDLLSANCDEFIFYDDLVHEQ
                     EAKQKRNAKKAPPKATTSGKKSEAGKGEDDKRQEALDFIVETVEALIAERGSDEKIWG
                     SLVKTTMQRR"
BASE COUNT         3821 a         5924 c         5660 g         3370 t
ORIGIN      
        1 gaaccagtga cccctgccgt gtgaaggcag tgctctaccg ctgagctaac cgcccctgtt
       61 aagggaggcg cgtattaaat cacagtcctt ccccacgggt caaatcgaaa cgacccggaa
      121 cggacgggat gcaagcctca tgataaaatt cgcgcctttc aacagttacc ggcatcccat
      181 gaccgtccgt acccgttttg cccccagccc gaccggcttc ctgcatatcg gcggcgcgcg
      241 caccgcgctg ttttcctggg cctatgcccg caagcacaat ggaaaattca tcctgcgcgt
      301 cgaggatacg gacgtggcgc gttccacgcc ggaggcggtg caggcgattc tggatggcat
      361 gagctggttg ggcctgaact gggacgaagg cccgttctac cagatgcagc gcatttaccg
      421 ctataaggaa gtgatccagc agatgctgga cgcgggcacg gcctaccact gctatacctc
      481 gccggacgaa ctgacggcga tgcgcgaggc gcagatggcg gcgggagtga agccacgcta
      541 cgacggcacc tggcgcccgg aaccgggcaa gtcgctgccg acgccgccgg ccggcgtgcc
      601 gccggtggtg cgcttccgca acccgcagag cggcgtggtg gcgtgggacg accaggtgaa
      661 ggggcgcatc gccatcagca acgaggagct ggacgacttc atcatcgccc gcgccgacgg
      721 cacgccgacc tacaacttct gcgtggtggt ggacgactgg gacatgggca tcacccacgt
      781 catccgcggc gacgaccatg tcaacaatac gcctcgccag atcaacctgc tgcacgcgct
      841 gggcgcgacg gtgccacgct atgcccacct gtccatgatc ctgggcgacg acgggcagaa
      901 gctgtccaag cgccacggcg cggtgagcgt gacgcagtac gacgatgacg gctacctgcc
      961 ggaggcggtg ctgaactacc tggcgcgact gggatggagc cacggcgacg acgaggtatt
     1021 cgacatggcg cagttctgcc agtggttcga cctcgaccat atcacgccct cggcggcgca
     1081 gttcaacacg gaaaagctga actggctgaa tgcgcactac ctgaagcagg ccgacctgcc
     1141 acgcatcgcc ggcgaggtat ccgggcggct ggcggcgcgc ggcatcgcca tcgaaggcgg
     1201 aacggacatg cagaaggcag tggaactgta ccgcgagcgc gcttccactc tgaacgagct
     1261 ggccgacgcg gtggaatact tttaccagcg catccatccg acgcaggaac tgctggacaa
     1321 gcacctgacg ccggaaatcc ttccggtgct gcagacgctg gcaacgcagc tcgctagtct
     1381 ggagtgggcg gtggagagcc tgcatagcgc catcgagcac gcggtggtat ccaacggcct
     1441 gaaattcccc aaggtggcga tgccgctgcg cgtgctgctc accggcggcg cacagtcgcc
     1501 gagtatcgac gcagtgatgg cgttgctggg gaaggaagaa gggctgaggc gcctgaacga
     1561 atacctggcc tgaggccgca ccttcagacc ggcgtgagtg ggatgacctt gtgctcgcgg
     1621 cgcagcttgc cgccctgggc gtagttggcg cgtacataat cctcgacaat ggcctggaat
     1681 tcctcggcga tagcgttgcc cttgagggtg acggtctttt cgccgtccac gaacaccggc
     1741 gccaccggca cttcgccggt accgggcagc gatatcccga tattggccag cttggactcg
     1801 ccggggccgt tgaccacaca gcccatcact gccacgctca tgccttccac accggggtac
     1861 tggctgcgcc acaccggcat ctgctcgcgc aggtagcgct ggattttctg cgccagctcc
     1921 tggaaaaagg tcgaggtggt gcggccgcag ccggggcaag cggtgactag cggcgtgaac
     1981 gagcggatgc ccatggtctg caggatttcc tgcgccacca ccacttcggt ggtgcgcgac
     2041 tcgcccggct gcggcgtcag ggaaacacgg atggtgtcgc cgatgccttc ctgcagcagc
     2101 acggagagcg cggcggtgga ggcgacgatg cccttggagc ccatgccggc ttccgtcagg
     2161 ccgaggtgca gtgggtagtc gcaacggtgc gccagttcgc ggtagaccag gatcaggtcc
     2221 tgcacgtcgc tgaccttgca ggagatgatg atgcgatcgc ccgccagccc ccactgctca
     2281 gcctgctgcg cgctttcgag cgcggactgg atgagggctt cgcgcatcag ggcttcggag
     2341 gagagcggtt cggcgcgttg cgcattttcg tccatcatgc gcgccatctt ggcttggtcg
     2401 aggctacccc agttgacacc gatgcgcacc ggcttgtcat agcggatcgc catcccgatc
     2461 atggcggcga actgctcgtc gcgcttcgag cccttgccga cattgccggg attgatacgg
     2521 tattttgcca gcgcctcggc gcattcaggg taagcactaa gcagcttgtg cccgttgaaa
     2581 tggaaatcgc cgatcagcgg cacgtcgcag cccaaggcat ccagttgttt ccggatcgcc
     2641 ccgacccggg cggcggcttc gggcgaattg acggtgatgc gtaccagctc ggaaccggcc
     2701 tgccacaact cgtaaacctg tttgaccgtg gcctcggcat cggcggtgtc ggtgttggtc
     2761 atcgactgca ccaccaccga ggcatcgccg ccgagtgcta tatcgccgac caggacacgg
     2821 cgggtgccgc ggcggtcaag gcgagactgg accatggaca tcagcgatta ttccagcgtg
     2881 aagcgcgcaa cattggctct ggcgtggggg gcaaggtcga ccaccttgcc attgaatgtc
     2941 atctggacgc cttctacgtt gccgaccacc agtttcagcg ggggcacgcc ttcaatcagt
     3001 ttcgtatcgc cggaaaaacc ggtgttctca tatatgacct tgcccgcgcc gtcgacgaca
     3061 ctcatccaga cctggtcgct gaaaactaca tccagccgcg caagagtttt tgcgggtacg
     3121 gcgggtacgg aaggcgcgac aacagccgtc tgcgtcgcag gaggctcttc caccaacgcg
     3181 gggactgggg tcgggcttgg gggcgaagtc gcttccagtt ccggcaccgg cgtcggttcc
     3241 gggatatcgg caagaggcac cgctgccggg gaagtttcct cctgctgccg gctggcaggg
     3301 gcggatgcct gctgcgccgg cgcgttctcc cgatagtcca tgtaaaccat ccaccaggcc
     3361 agcgccagca agaggagaag agcgccgagc acatacggca accaatccct cctgccgcgg
     3421 ccgataatca cgatgttttc cgactgcagg gcgatcgaat gatgcgccga aggcatggcg
     3481 gaacggtagg ccagcaacag gggctccggg tcgatctgta gcagacgggc atagttacgg
     3541 atgaacccac gaacgaaagt gacatcgggc agggatgcgg tgtcgccagc ttccagcgcc
     3601 agcaactgat tcctgctaag ccgcagcttg cctgccgcat cctcgatgct catgccgcat
     3661 tgttcacgcg ccgcggtcag caaagcgccc gccccgaccg ggcctgtgcc atcatccgtc
     3721 atcacgccgc cttcctgtgt tgattcgctc atttttgtcc ggacagatac gccttggcct
     3781 gctctgattt aggatacttg tttttcagca gcatgccata gctggcctcg gcgccggcat
     3841 cccccaacgc ccgttcgatg cgcacaccca gccacagcat ctccgcggtt ggctcagcac
     3901 aacgggtcga ctgattgaac tcctcccgcg ccctggcata ctctgcgcgc ccatacgcga
     3961 tctgggcaag ctgataggac gcctggcaca tgccgggccg cagctccagc gcgcgcagca
     4021 ggtaggtttc ggccgcactg tcgtcaccct tcttaagcac acacctggcc gcgttgaggt
     4081 aagccatttc cggggtatcg tacaaggggt tcttgagtgc cagcttgaac tcgctgatgg
     4141 actcgtcgat gcggttgcgg gcgcagagaa acgcgccgta gttgttatgc atgtccgagt
     4201 ttgtcggatc cagttgcagt gatttcctgt aattgctctc ggccttggcc tcctcgttca
     4261 gtgccgtata caccagcccc agtccaccgt aggccggcgc gtactccggg tcgatgctga
     4321 tcgcttcgct gaactcttcc agcgcaaccg cctgcatgcc gcgggcgtaa taccccgagg
     4381 cgagttcggt atgcaccttg gcgcgattgc gcgccgaggt ttcctgtttg ctgacattgt
     4441 aagggttgac acgttccgtt acgcatccgg ccagggctac gaccatccac aacagcaaca
     4501 gcggtttttt catgcggcgg cctccagaac cagtttatgt gtgcgtctgg tcttgtcttt
     4561 gacttttcct gccaactgcc cacacgccgc gtcgatgtcc tcgccgcgcg tcttgcgggt
     4621 agtcaccacg tagccggcct gcatcagcac gtcgcggaag cggcggatat tctccggcct
     4681 ggaggtctcg tagccgctgt cggggaaggg attgaacggg atcaggttga acttgcacgg
     4741 cacgtctttc accaggttca acaactgcct ggcgtgctcg actgtgtcat tgaccccatc
     4801 cagcatcacg tattcgaagg tgacaaaatc gcgcggcgcc ttttccagat agcgccggca
     4861 agctgccatc aactcggcca gtggatattt cctgttgatc ggcacgatct ggtcacgcag
     4921 cgcatcgttg ggcgcgtgca gcgataccgc cagcgccacc ggacaggcgt cgcgcaaccg
     4981 gtccatggcc ggcaccagac cggaggtaga taccgtcacg cgacggcggc tcaggccgta
     5041 ggccgagtca tccagcatga tattgagcgc ggtgacaacg ttgtcgaaat tggccagcgg
     5101 ctcgcccatg cccatcatga ccacgttgga gatgacgcgc gcccctttcg ggtcgcgccc
     5161 cagggcttta ttggccagcc acagctggcc aatgatttcc gacacgtcga gattgcggtt
     5221 gaaaccctgg cggccagtgg agcagaacgt gcactccagc gcgcagccaa cctgggagga
     5281 cacgcacagc gtaccgcggt cggcctcggg gatgaatacg ctttcgacgc cgttgccggc
     5341 accgacatcg atcagccact tacgcgtgcc gtcttcggaa atttgctcca gcttgactgc
     5401 aggcggtcgg atttcagcca cctgcgcgag tttttcgcgc aggctcttgg cgacatcggt
     5461 catctgcgca aaatcgttca cgccgaagtg gtgcatccag cgcatcatct gcttggcacg
     5521 aaacggcttc tcacctaact cggcaaaaaa ttcggcgagc ctgggctgat cgaaatcgag
     5581 cagattgacg gtcattcaga cttatttgcc gaagaaatag gcaatttcga tggcagcgtt
     5641 ctctgcggaa tcgctgccat gcacggcgtt ggcatcgatg ctttcggcga aatcggcacg
     5701 gatggtgcct gccgccgctt ccttgggatt ggtggcaccc atcagttcgc ggttcttggc
     5761 aacggcattc tcgccttcca gcacctggat catcaccggg ccggaaatca tgaacttgac
     5821 cagatccttg aagaaagggc gctccctgtg taccgcatag aagccttcgg cctcggcttg
     5881 cgacagttgc gccatcctgg cggcaacgat cttgagaccg ttggtctcga agcgggaata
     5941 gatcttgccg atcacgctct tggcaacagc gtcgggtttg atgatggaca gggtacgttc
     6001 aacagccatg gtgactccag aaagttaagt gaatgaaaga tcgataaaat accattttgg
     6061 ccgcccgccg tccatttcaa gctgacgttc gcgctagtca ggcttgtcgt ggaacgtcct
     6121 gtttccggca tttctgccga taacgatagg ccagcagaat cgccaaccca gcaacgttgg
     6181 gcagccagac ttccggcgtc gcccacggaa cgccatcaat ggtgaaatcg gacagtggcc
     6241 agaaaatggg cgtgggaaaa taacgcaaac tgtggaaggg aaaatccagc acgatatgga
     6301 acggccacgc cagcatggcg aaagcgatgt cgcgccggaa cagcgccacc agccccacca
     6361 ccggcaggct gatgacgaag ctgtgccaga gatcatagga gacgaacagc cagtgcggca
     6421 gggtatgcag cagcggcggg ccaaaatggt agctgccgtc ccatatcctc accaccatca
     6481 gagcgccgaa ggaaaccagg tccggcagcg ccccgaacag catggccagc caagggtggc
     6541 tgcgctggcc gaacaatccg tacccccaca gagcatggct gaacgtatcc aagaaagccc
     6601 ttcctttccg tcttcgagac atgcattgta tgccaaacac gatgccgctt gagcgaagcg
     6661 gcatgctgta ttaggctggc agtcattgcc agccttgcta agattgaaat ccatgcgccg
     6721 catcctcatt tttctcatcc tcctggccgc tgccgccagt gccttctggt ggttcacccg
     6781 ccccaagccg ataccggtgg tgctggtgga ggtcgggcgc ggcacggtgg aagccagcgt
     6841 ctccaatacc cgcgccggta ctgtggaagc ctgccagcac accaggctgt cacctattac
     6901 cggcgggcgc atcgcctata tgggcgtcaa gaaaggcgac cacgtcaaaa aggggcaggt
     6961 gctgatccgt ctgtggaatg acgaccagca ggcacaaagc ggcttggcgc agacccaggt
     7021 cgaatcggcg cgcaagcggg tgggtgaaat ctgcgccatg gcagatagcg ccgaacgcga
     7081 agcggcgcgc atgaccaagc tgcgggtcaa gggcttcatt tccgaaggcg gcgaagaaaa
     7141 agcccgctac gaagcgcaat cccgtcgcgc cgcctgcgag gcgatgagcg cagacgtcac
     7201 acaggcccag gcgcgggtga ccgtaacgcg cgtagaacag ggtcgtaccg ttctggtggc
     7261 gccgttcgac ggcaccgtgg ctgatatcgt cggcgaactg ggcgaataca ccacaccctc
     7321 gccgcccggc gtggcgacgc cgccggctat cgatctgatc gacgactcct gtctctacat
     7381 tgaggcgccg atggacgagg tggatgcacc caagatccgc cccggccagc cggcccgggt
     7441 cacgctggat gcgctgccgg gcaaggtgtt gccggcgcac gtcaagcgcg tcgcgcctta
     7501 cgtggtggcg gtggaaaaac aggcgcgcac cgtagacgta gaggccaatc tcgatcagcc
     7561 gccgccgccc ggagaactgc tggtgggcta cagcgccgat ctggaggtca tcctcgacag
     7621 ccgcgacaac gtgctgcgcg tacctacctc ggccctgctg gagggcgaca aggtgctgct
     7681 ctaccaagcc gccagccgca agttggagga gcgctccctc aagaccggca tcgccaactg
     7741 ggaatatacc gaggtgctgg aggggctgaa cgccggcgac cgcatcgtca cttcgctgga
     7801 acgcgatggc gtgaaagccg gcgcgctggc caggctggaa gaaaatcccc cggccaaata
     7861 ggctgaacga ccgatgcgcc tgatcgaact cagcggcata gcacgcactt tccagctagg
     7921 cgacagcaaa gtgcacgcgc tgcgcggcat caacctcggc atcgcgcccg gcgagtatgc
     7981 tgccgtcatg gggccgtccg gctcaggcaa atccaccctg ctcaacctgc tcggcctgct
     8041 cgaccgcccg gatgccggca cttattttct cgaaggccgc gatgtcacca cgctgagccc
     8101 ggacgagcag gcgcgggtac gcagcgagcg catcggcttc gtgttccaga gcttccacct
     8161 ggtgccgcgc ctgaccgccg ccgaaaacat cgccctgccg atgatgctgg ccggtatcgc
     8221 cacggcggaa cgcggccgcc gcgtggcaca ggcgctcaag gactacgggc tggcagaccg
     8281 tgccaaccac cgcccggacc agctttccgg cgggcagcgc cagcgcgtcg ccatcgcccg
     8341 cgccaccgtg atgcgcccgg ccatgctgct ggccgatgaa cccaccggca acctcgaccg
     8401 cgccaccggc gaggaagtca tgcgcctgct ggaggcgctg aacagccagg gcgtcacgct
     8461 gatcgtggtc acccacgacc aaacactggg cgcccgcgcc catcgccaga tcgccatgct
     8521 ggacggcgcc atcgccagcg atacggcaac ctcatgaacg tcgccgatac cctgcgctac
     8581 gcccgtgccg ctgccagcgg taccccgctg cgcaccggcc tgctgatcct cgccatgtcc
     8641 atcggcgtgg cggcggtcgt catgctcacc gcgctgggcg acggcgcgcg ccgctacgtg
     8701 gtagaagagt tttccgccat cggcagcaac ctggtcatcg tgttgccggg gcgttcggaa
     8761 acgcgcggct tcaaccccgg caatctagtc accagcacag tgcgcgacct gacggtggag
     8821 gatgccgaat ctctgctgcg cgtgcccggc gtggtgcgcg tcgcgcctgt gatcatcggc
     8881 accacggaaa tcaacgcgcg cggaaaactg cgggaaacca tgctggtcgg cagttccagc
     8941 gacttcatca aggtgcgcca cttgaagctg gcccaggggc gtttcctgtc gcatgatgcc
     9001 ctgggccacg gctcggcaga agtggtactg ggcgcgctca tcagcagcga actgttcggc
     9061 agcgaaaacc cgctgggaaa gacggtacgc atcggcgacc gccgcctgcg cgtggtcggc
     9121 gtgctgaccg agggtgggcg cggcatgggc atgaccacca acgagctctt gatcgtgccg
     9181 gtggcgacag cacaggccat gttcaacagc aacaccctgt tccgcatttt ggtcgagacc
     9241 agaagccgtg ccacagtcga aagcaccaag gcggcagtgc tcaagaccat cattgcccga
     9301 cacgacgacg aggaggacgt gaccgtgatc tcgcaggatg cggtattgca gactttcgac
     9361 aagatcctcg gcgtgctgac cctgggcgtg gccggcatcg ccgccatcag cctggctgta
     9421 gccggcatcc tggtgatgaa cgtgatgctg gtgtcggtga cgcaaaggac ggcggaaatc
     9481 ggcctgctca aagcgctggg tgccactgcc cgcgccatac gcgccgcttt tctcaccgag
     9541 gccgccatac tgtcgctggt cggcgccgtc atcggtgtgg tgctgggcga agccgccgcc
     9601 gccgtactac gccacctcta ccccaccttc cctgcctacc cgccgccgtg ggcgatcgtc
     9661 gccggcgtcg gaaccgcact ggtgaccggg ctggtgttcg gcatcatgcc tgcacggaag
     9721 gcggcgcaga tggatccggt gcaagcgtta tcgaagcggt aaattccgga gaacaccgaa
     9781 acatagccgt ccgctattaa gaacataaca acaatcaatt ggagctatcg ttatggctgt
     9841 actatcattt ccctgcattc aaccaaacta ctgacaggag gccgttatga acgtgcaaat
     9901 cgacatcggt atcaacgcca aagaccgcaa gaaaatcgcc gagggacttt cacgggtgct
     9961 ggccgacacc tacacagtct acctcaagac ccataatttc cactggaacg tcaccggccc
    10021 gatgttccag accctgcacc tgatgttcat gacccagtac aacgaactgt ggctggcgac
    10081 cgacctgatc gccgaacgca tccgcgccct gggcttcccg gcgcccggca cctacgccga
    10141 attcggaaaa ctgacctcga tcaaggacac cacgggcgta cccaaagccg aagacatgat
    10201 ccgcctgctg gtggaaggca acgaagcagt ggcacgtacc gcccgcgaag tgttcccggt
    10261 cgtggagaaa gccagcgacc agcccaccgc cgacctgctc acccagcgca tgcaggtcca
    10321 tgaaaaaagc gcctggatgc tgcgcagcct gctggagtag gagcgccgcc ctcggcgcga
    10381 cttgcgctga gatgaatcgg ggcgagggcg cccctcctac acgatcatct tggcggctcc
    10441 atcccgtttt tgagataaaa tcgcaacaat caacccatca ggagccacac ccaccatgtc
    10501 ccccaagcac gcccgtctgc tcatcctcgg ttccggcccg gctggttatt ccgccgccgt
    10561 ctacgccgcg cgcgccaacc tcaagccggt actgatcacc ggcatggcgc aaggcggtca
    10621 gctgatgacc accaccgaag tcgacaactg gccggccgac gtcaacggtg tgcaggggcc
    10681 ggagctgatg gcgcgcttcc agcagcacgc ggaacgcttc aacaccgaga tgatcttcga
    10741 ccacatccac accgccaaac tgaccgaaaa gccgatccgg ctgatcggcg acgccggcga
    10801 atacacctgc gacgcactca tcatcgccac cggcgcctcg gccaaatacc ttggcatccc
    10861 atcggaagaa aaattcgcag gcaagggcgt ttcggcttgt gccacgtgtg acggcttttt
    10921 ctaccgcaac caggaagtgg cagtgatcgg cggcggcaat accgccgtgg aggaagcgct
    10981 gtatctggcc aatatcgcca gcaaggtgac gctggtacac cgccgtgaca agttcaaggc
    11041 cgaagccatt ctggtcgaca agctgatgga gcgcgtcaag gagggcaaga tcgtactcga
    11101 actattttcc actttggacg aagtgctggg cgacgacagc ggcgtgaccg gcatgcgcat
    11161 caagagcgtc gacactggtg caaccaagga catcgctctg cagggtgtgt tcatcgccat
    11221 cggccacaaa ccgaacaccg acatcttcga aggccagctg gaaatggagg gcggttacat
    11281 cgtcacccag agcgggcgca tcggaaacgc caccgccacc agcattcctg gcgtgttcgc
    11341 cgccggcgac gtgcaggacc acatctaccg ccaggccgtg accagcgccg gcaccggctg
    11401 catggcggcg ctggatgccg agcgctacct ggaccacctg ggccattaaa ccgtgaaacg
    11461 gccggcaacg cctccgtcca ccatcgacga agacgaagct ggcctgttcc aggaggccgt
    11521 caagggcgtg cgtcccctca agcagccgcg cgcccacctg cccacgccta aaccccgccc
    11581 caccccgcag cagttgttcc gcgacgaatg ccaggcgctg gccgactcct tgtcggacgg
    11641 cttcatccct gcgcacgaga tggaaagcgg cgaggagctg ctctacctgc gcgacggcca
    11701 ttcccccgac attttgcgta aactgcgtcg cggccactgg gtagtgcagg ggcagcttga
    11761 cctgcacggc atggtagtcg aagaagcgcg cgcccatgta gcggaattcc tcacacactg
    11821 taaaaagcgt ggcacgcgtt gcgtgcgcat catccatggc aagggactgg gttcgaagaa
    11881 ccgcgagccg gtactcaaga acaagctgag aagctggctg atgcagcgcg acgaagtgat
    11941 tgcctattgc caggcgcgcg cagtggatgg tggcagcggc gcagtagtgg tattgctcaa
    12001 agccggctca tgacaaaagt cagccggaaa tagccggtcg caaattcatg gctcaccctc
    12061 agcgggatta aattgacgct gaggaacacc cgtaatagta tgagccgctt cactggcctg
    12121 gcgggaaacc agaagccaca cccctacttt atcgtagctg aatttaatta atggagattc
    12181 atcatgcgta acaaaatgat cttgcttggc ctggtgtatg ccgcattctg cggacttctg
    12241 ccgattgctt cccatgccga agatgccgca atgggcgagc ccgtgacggt agatatcccc
    12301 ccaggcactc tgccaacagc cacgctggaa gtggaagcca cgcagatccg cctgctcatc
    12361 ggcggtgcca agggcaaggg cgtgctgcac ttcaacggca cgaactaccc attcacgctt
    12421 tcggccggca gcataggcgg ggtcggttac gccgaggtca aggcgactgg ggaagtctat
    12481 ttcctggaaa agcccgagga cctgaacggt gactacagcg ctgccaccgc aggcgtcacg
    12541 gcagtcaagg gtgcagggat gtctactttc aagaatgccg ggaacaaagt gaaactcagc
    12601 atcaagtccg agagcgaagg ccttgggctg ttcctgggta tcggcgccat gaaactcgaa
    12661 ctggacaagt aatccatttc aggtgctgcc cggggcatga ccaggagcag cacctgaaat
    12721 tatctccctg actcaatcag gcataccgcc tcggcagcga tgccttcgcc gcggccggtg
    12781 aatcccaggc gttccgatgt ggtggccttg acgtttacgc aatcggcagc aagcccgagg
    12841 tcggcggcga tgttggcaca catggcaata atgtgcggcg ccagcttggg cgcttcggca
    12901 atcacggtag catcgacgtt gatcacgccg tagccttttt ccttcagcag ggccgagaca
    12961 tgtcgcagca gttgccggct gtcgatgccc ttgaagcgcg ggtcggtgtt ggggaaatgc
    13021 ttgccgatat cgcccagcgc cgccgcgccc agcagggcat cgcaaatggc gtgcagcagc
    13081 acatcggcgt ccgagtggcc gtccaagccc ttatcgtggg ggatatccac cccgccgatg
    13141 atgcacttgc gccccgccac cagcgggtgc acgtcgaaac cgtggccgat acgtatcatg
    13201 tgttctttcc cgcgttatcc agaatcaacc cggccatcac caggtcttcc gggtaggtta
    13261 ccttgaggtt atgtccgtcg cccagcacca gtttgggggc gtggccgagg aactccaccg
    13321 cttgcgcctc gtccgtgggg atgcctgtcc ccattgcccg cagggcgtcg cgcaacagcg
    13381 cgtagcggaa catctgcggc gtttgcgcgc gccacaggcc ttcgcgcggc tcggtacatt
    13441 gtacgcggcc accggcgtct tcacgcttga tggtgtcggc caccggcagc gccagcagtc
    13501 cgcccacggg atcgccctgc aacatctgca gcaaccggtc cagcagctcc ggcgagaggc
    13561 aaggccgcgc cgcatcgtgc accagcaccc agtcctcccc gctcacttcc ccggcaatca
    13621 cgtcgaggcc gttgagcacc gtttgcgccc gcgtttcgcc cccgcagcgc agcacggtta
    13681 ccctgtcgtt gccatacccc ggccaggcag tatcttccgg gctcagcacc acaaaaatac
    13741 cggtaatggc aggatgcgca cagaacacgg aaagcgtatg atgaatcaac ggcttgccgt
    13801 ggagggggag atactgtttg gggagttcgg aacccatgcg gctaccgctg ccagcggcag
    13861 gaatcaggac ataatttttt ggcatgcaaa atcataacat gccccttgat tccggccatt
    13921 ctggccatac tttattagta gatgctaatt caggagatgt ccatgcaatc catccgcccc
    13981 gccgctgtcg ccggtatgtt ctaccccggc aatccggtca cgctggcgca cgatatcgac
    14041 gccatgctgg tggaggcgga agcgcgtgcg cctcggctgg cgcccaaggt gctgatcgca
    14101 cctcatgccg gctacatcta ctccggcccg gtcgccgcca gcgcttacgc cctgctcaag
    14161 ccggtggccg agcgcattcg ccgcgtcgta ttgctaggtc cgacgcaccg tgttgccgtg
    14221 cacgggctag cgctgccggg ggcagaagct ttcgccacgc cgctgggtac ggtcgagatt
    14281 gacttggagg cagccagcct gatcgccgcg ctgccgcaag tcgtgactag cccacaggca
    14341 catgcgcagg aacactcgct ggaggtgcat ctgccgttcc tgcaatcgct gctgccggat
    14401 ttcaaactgc tgccgctggc ggtgggcatg gccagcgccg aggaagttgc ggaggtgctg
    14461 gaaatcctgt ggggtggcga tgaaaccctg atcgtcatca gctcggatct gtcccaccac
    14521 ctgccctacg ccaccgcgaa gcgggtggac ggtgccaccg cgcaggccat catgcaaaac
    14581 cagccggttt cgcacgagca ggcctgcggc ggaacgccag tcaacggact gcttctggcc
    14641 gcacggcatc atcacctcac cccatacctg ctcgacctgc gtaattctgg cgataccgcc
    14701 ggcagcaagg accgcgtggt gggctatggc gctttcgcct ttgccgggga ggcgcggcaa
    14761 tgaccgagca ggaacacgga gcaaccctga tccggctggc acgtggcgcc attgccgaat
    14821 gcttcggcct gacagtaccg cccgtaccta ccgatgagga ctggctggcg gtgcccggcg
    14881 ccacgttcgt caccctgacg gaggatggca acctgcgcgg ctgcatcggc agcctggcgg
    14941 cccagcggcc gctggtcgac gacgtgcgcg ccaacgccgc cgctgccgct ttccatgatc
    15001 cgcgcttcga gccgctgcgg ccggaggaac tggatcgcac cgcgatagag atctccctgc
    15061 tttcgcccac gcagaccatg cagtttgccg acgaacgcga cgctttggcg caactgcgcc
    15121 cgggtatcga tggcattgtg ctggaatacc agcatcaccg cggcactttt ttgccgcagg
    15181 tatgggaaca gctgccgatc ccggaggcat tcatgaccca actcaagcgc aaggccggtc
    15241 tgccgggaaa tttctgggcg ccggaagtca agctggcgcg ctataccgtg cagaaatgga
    15301 aggaaaacta accatggacg aagccatcca catcgacgag cgctatccgg cgcaattctg
    15361 gcaccgcctg gatgacggcc gcatccagtg cgacctctgc ccacgcgact gcaagctgca
    15421 cgaaggccag cgtggtgcct gcttcgtgcg cggcgcagcg gatgggcaga tggtgctcac
    15481 gacctatggg cgaagttccg gcttctgcat cgacccgatt gagaagaaac cgctcaatca
    15541 cttctatccc ggttccagcg tattttcctt cggcaccgcc ggctgcaatc tggcctgcaa
    15601 gttctgccag aactgggaca tttccaaatc gcgcgacatg gaccggctga tggaccaggc
    15661 ctcaccggaa gccatcgccc aggcggccaa ggcgcacggc agcaagagcg tggcctttac
    15721 ctacaacgac ccggtgattt tctacgaata cgcgctggat acggcggacg cctgccacgc
    15781 gctggggatc gagaccgtag cggtaacggc gggttacatc cacgctgcgc cacgcaagga
    15841 atttttcgcc aagatggacg ccgccaatat cgacctcaag ggttttaccg aggatttcta
    15901 cctcaagctc accggatcgc acttgcagcc ggtgctggac accttggcct acgtgcacca
    15961 cgacaccggc tgctggctgg agatcaccac cctgctgatc cccggccaca acgactctga
    16021 cgcagaaatc accgccctct gcaaatgggt cgcgcaggaa ctggggccgg aggtgccgct
    16081 gcacttcagc gccttccatc cggactggaa aatgtccgat gtgccggcta cgccgcccgc
    16141 cactctcagc cgcgcccgac gcatcgcgct ggatgccggc ttgcactacg tctataccgg
    16201 caacgtgcat gatacggagg gcggcacgac cttttgccct tcctgccgcc atgccgtcat
    16261 cgtgcgcgac tggtacgaca tccgccacta cgacctcact ccgcaaggag cttgtccacg
    16321 ttgtggaact gccgttgccg ggcgcttcgg ccattacagc aagccgttcg gttcaaagcg
    16381 cattccggtg agaatcgcca cagacctgaa ctgaccagcg ctccgccggg gggcggtaat
    16441 aaggattgca aaacagcggc agcgataggc gtatgatgca gaattacctg atgaaattca
    16501 gggatcattc accagaagga aatccgcatg accaatctgg ccgaagcact caacaaggaa
    16561 ctcgcggagt atgaagcgac cctcgcactc agcaatggtg aatcgctacg gatcaaaggg
    16621 gacaccacct ccatctccga tcagttcatc agcgtcagct tgtccaacaa ccccgacaaa
    16681 aaaggcagga agctgatcaa catcgaccat atcgtgagca tcgactggta caccagatag
    16741 tcagccccgg aagcgggtca acatttcaaa tggaggcatc gcaatgcgca agaaactgat
    16801 caatgatccc ttccaggaat ccgaagtggc ggaccatgac tggttgcagg ttcatgactt
    16861 ggcaggggtt gaactgacgt ccgaagatgc cgcgcatccg atcgaatcgg tttttcaggt
    16921 ggctacggct tccggctggc gtgcagccga gcctggcagt caaaccatac ggttgttgtt
    16981 cgaccagccg cagcacctgc ggctgattca tctggatttt gtggagcctg cggtggagcg
    17041 cacacaggag tatgtcttgc gctggtcgcc cgacaacgga cagacgtttt acgagatcgt
    17101 gcggcagcag tggaacttca gcccgtcagg tacccgttcg gagacggaaa accacgcggt
    17161 cgatctggcc aatgtcacca tgctggaact gaccatcaat cccgacattg gcaaccggaa
    17221 tgcgactgcc tcgctacaac gctggcagat tgcctgaccg accgagcaag acctagtagc
    17281 ggcgccttgg agcggccgcc gggccacggc cttcgatatg gcggaacgtg atgcgcccct
    17341 tgctgacgtc gtagggagac atttccagcg tgatcttgtc gcccgcgagg atgcgaatgt
    17401 ggtgtttgcg catcttgccg gcagtgtatg cgaccaactg gtggccattg tcgagcgtga
    17461 cgcgatagcg cgagtcgggc agcacttcca tgaccacgcc attcatttca atgagttctt
    17521 ctttggccac gatagcctct ccgcttggta gcaataaaaa agcccggtgt tagccgggct
    17581 tttccggtga gactgccgca cgtagcggca atcccgttgg tacagtgact tactcagcag
    17641 cgcgaatgtt cgacgcttgc ggacccttgg ggccagtggt ggagtcgaaa ctcacgcgct
    17701 ggttttcctg cagggtcttg aaaccttgtg cctgaatagc cgaaaaatgc acgaagaggt
    17761 cagcgccacc gccatcgggg gtgatgaagc cgaagccctt ggcgtcgttg aaccacttta
    17821 cggtacctgt tgccatgtct tgatatcctt aaaatcaaat gaaaagggac ttgcccttgt
    17881 gggcgaaggt caagacggcg agatgatgaa ggaagaaaac ccggaaaaac ggaagggtac
    17941 tgaacctgac agcaacaaca ctacttgaag atcgctggcg cgcactatgc gcttaattct
    18001 ctgcttatgc aaatttaatt gcaattgcac cgaaaccgat tgtcatcaac aatgtaccag
    18061 aacgcagact tcatacaaag gaacgtaata tggccggcca acccgaaatc agcaacatgg
    18121 cgctgttctg cgatttcgag aacatcgcgc tgggcgtgcg cgacgcccgc tatgcgcagt
    18181 tcgacatcaa gaaagtgctg gagcgcctgc tgctcaaggg cagcatcgtc gtcaagaaag
    18241 cctattgcga ctgggaccgc tacaaggaat tcaagtccac catgcacgaa gcggcgttcg
    18301 agctgatcga gattcctcat gtcaggcagt ccggcaagaa ctcggcggac atccgcatgg
    18361 tggtggatgc gctcgacctc tgctacacca aggcgcacgt ggacgccttc gtcatcatca
    18421 gcggcgattc cgacttctcg ccgctggttt ccaagctgcg cgagaacaac aaatacgtca
    18481 tcggcatcgg cgtcaggaac tccacctccg acctactcag cgccaactgc gacgagttca
    18541 ttttctatga cgacctggtg cacgagcagg aggccaagca gaaacgcaac gccaaaaaag
    18601 cgccgccgaa ggccaccacc agcggcaaga agtccgaggc tggcaaaggc gaggacgaca
    18661 agcgccagga agccctggat tttattgtag aaacggtgga agcgctgatc gccgaacgcg
    18721 gttccgacga gaaaatctgg ggctcgctgg tcaagaccac catgcagcgc cgcaa
//
LOCUS       DAOMFA010000009        18432 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_208_length_18432_cov_5.598574, whole genome shotgun sequence.
ACCESSION   DAOMFA010000009 DAOMFA010000000
VERSION     DAOMFA010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 18432)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18432)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18432
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_208_length_18432_cov_5.598574"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            28..1920
                     /locus_tag="PKW44_01035"
     CDS             28..1920
                     /locus_tag="PKW44_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HQR50204.1"
                     /translation="MESFREHSKGWLAKLILAFITVPFALWGIDSYLQGAGSNVAVAK
                     VNGQNITVQEYDNALQTLRSQLQQEGKTDPALLDDPAVKQSVLNRLIVSRLLTGEVRS
                     ANLVISDEQLGKTILEMPEFSQNGKFSQEVYDSVLKQNNLTPTQFESRMRGELLTKKL
                     RDGVTATAFAPHAVSDNLIQIDRQQREVSIAAIKAADFLGQAKVDAAQVQAFYDKNKD
                     KFRVPEQVKIEFVIFSSNNLITSTPVAEDEVKKFYQENASKFQGDEQRRASHILVAFD
                     KKDDTAKKAAHEKAESVLAEVNKNPAKFDELARKYSQDPGSAANGGDLGLFGRGMMVK
                     PFEDAVFSMKPGAVSGLVESDFGYHIIKLTEIKGQAPTYDDVKAQIRAELLNQKALAK
                     FAEQAEGFNNMVYEQSDSLQPVVKAFNVPLQTSQWMSREDAMKFFKSDKLVKAIFSDE
                     VLKDKRNTEAVEAAPNTLVAARVVDYKPAAPRSFAEVAPAIEEHLKREQAFAMAVKKG
                     EADLAQLRAGREVAGLEWIPPVVIARDNTQGLSEGVVQQAFKIDADKLPAYGSLQNQG
                     ADYTLIRVSRVESSAPQDEAEKTTLRSGIQTALAAEYLAAYIGSLRYKANITTNQQLL
                     GAGAQQ"
     gene            complement(1994..2779)
                     /gene="fabI"
                     /locus_tag="PKW44_01040"
     CDS             complement(1994..2779)
                     /gene="fabI"
                     /locus_tag="PKW44_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020182017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HQR50205.1"
                     /translation="MGFLTGKRALIVGLLSNRSIAYGIAEAMRREGAELAFTYQMEKF
                     QDRVGKLAAEFGSDLVFPCDVSDDAQIDALFSNLGKRWDGLDIIVHSVAFAPREALAG
                     DYLDSVTRDNFRIAHDVSSYSFAALAKAGRPMMEGRNGALLTISYLGAERTMPNYNVM
                     GVAKASLEANVRYMAQSLGPRGIRVNAVSAGPIKTLAASGIAGFDKMIAFNDKHAALR
                     RSVSIEEVGNVSAFLCSDLASGVTGEITYVDGGFNITAAATVE"
     gene            complement(2790..3608)
                     /locus_tag="PKW44_01045"
     CDS             complement(2790..3608)
                     /locus_tag="PKW44_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQR50206.1"
                     /translation="MKQALSNPLARWGFAIIATVLLLALLAPLIAPYDPDAIDVKAIL
                     LAPSSSHWMGTDGLGRDVFSRMLFGARISLLVGFVAVGIATAIGIVLGALAGFYRGWV
                     DTVIMRLVDVMLTIPTFFLILAVIAFLTPSIWNIMIVIGLTSWMGVTRLVRAEFLSLR
                     EREFVMASQILGARDGRLIFRHLLPNSLTPVIVSTVLGVASAVLVESGLSFLGLGVQP
                     PNASWGNILTDGKEYIEFAWWLSLFPGMAILLTVLGYNLLGEGLRDALDPRSNK"
     gene            complement(3605..4624)
                     /locus_tag="PKW44_01050"
     CDS             complement(3605..4624)
                     /locus_tag="PKW44_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQR50207.1"
                     /translation="MTRYLLRRLFWMVPLLVGISLISFLIMHLAPGDITTTEAGFNPK
                     ASQESRQKLRQLYHLDEPLIVQYGLWLKRMATLDFGTSFASHQRPVFLERKDDKGNVS
                     KGMIQEALPITLLINVLSLVLIIAVALPLGVISALNPNKPPDRTITVFVFIGFAVPGF
                     WLALMMMYWLGVAHDWLPISGLHSLGYEHLTWPARWWDTARHLAIPVFISALGGLAGI
                     SLFVRNGMLDVLHQDYITTARAKGLPENTVVYRHALRNALLPLITILGLSIPGLIGGS
                     VIAESLFAIPGMGKLFYDAVLMRDFPVIMGILTIGAALTLIGNLLADLAYAWADPRVR
                     RGVGG"
     gene            complement(4621..6261)
                     /locus_tag="PKW44_01055"
     CDS             complement(4621..6261)
                     /locus_tag="PKW44_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HQR50208.1"
                     /translation="MPRKLLPFLAAFFLLAACGRPADSSNADLNRPYPPQSGGTLVDA
                     MAGEPSGLIPMLAGESAASAIAANIFNTLLKYDKNLELEGELAKSWDISDDQKTITFH
                     LKPDLKWADGAPLTSADALFTWKLVTDDNTRTPYGADYKLVIRAEAPDAQTFRVSYAQ
                     PYAPALDSWAGLQILPKHLPQGQDINTTAFARHPVGSHYYQLAEWTNGERISLVRNPN
                     ATQGQARIDRLLSRFIPDPAAQFLELMADNIDSMGLSPIQYARIFPARPELNQKIALY
                     KELGNAYTYLGFNLKHKPFDDQRVRQAINYAIDKQEIIDGVLLGLGEPVASPYKPGTR
                     WSNPRLHPYPYDPEKAKQLLKDAGYADHDGDGILDKDGKPLAFEILTNQNKQREMSAT
                     LIQRRLKDIGIDVKIRVLEWASFIGRFIKTGDFDVVLLGWGLGQEPDQFNIWHSSQQA
                     PGQFNFIGYSNPEVDKLLEQGRLELNPDKRQEIYHQFAKILLEDSPIVYLYAGYGLPA
                     IHKRVKGIDNPAPPAGIGHNAYEWYIPEPLRRTEISAQ"
     gene            complement(6251..7888)
                     /locus_tag="PKW44_01060"
     CDS             complement(6251..7888)
                     /locus_tag="PKW44_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HQR50209.1"
                     /translation="MAVKHLLQLVILSLPYLAPAHADTNAAGDEITYYSDAQVYGRRD
                     SELFNYESLPQAPDSVQQLPPLVQDSGENGAAAPTQANSNDLWQRIKGGYAMPELRSP
                     FTATHESWYAARPDYVRRMVGRSQKYLYHIVEEVQKRGMPTEIALLPMVESAFNPQAN
                     STSRASGIWQFIPSTGKHFGLKQDWWVDNRRDVMAATDAALDYLQKLHVMFGSWDLAL
                     AAYNAGEGTVMRAIERNRKQGLPTDYQSLSLPAETKNYVPKLQAVKNIVTNPEQYGLD
                     IRPIANQPYFTTVQAPKQIDASLAAKLAGISQEEFLALNPEYNRPVLTSSGPTHEFLL
                     PVGKAEEFSANLAAYNQPLVSWQTYNAKRGERQDAIARKFGISLAQLRDVNDIVGKGG
                     KLSQPSILLVPAQTGGESALSVAETATQAPEQAGATPDTKHHVVKKGDSVNSVAKRYD
                     MSANSLVALNHLKSRKLRSGQVLLVRADIANESTVRTRASSAPEKIHYVVRRGDTLTE
                     IARKFDVATNDLQRWNRLSGDRLVPGHKLTIFKPDAA"
     gene            complement(7990..8754)
                     /gene="gloB"
                     /locus_tag="PKW44_01065"
     CDS             complement(7990..8754)
                     /gene="gloB"
                     /locus_tag="PKW44_01065"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HQR50210.1"
                     /translation="MLEVIPIPAFRDNYIWLLRRKGHAVVVDPGDAAPVLALLAEQQL
                     QLDAILVTHHHDDHIGGVTELVRTFPISVYAPRKGHYAFPHQPVGEGDCIDLTTLDIC
                     LQVLETPGHTLDHVAYYGANSLFCGDTLFSCGCGRLFEGSCEQLYASLQRLAALPLQT
                     RVYCAHEYTLDNIRFALAVDPGNADLALRKRDVERCRADGRPSLPSSLALECATNPFL
                     RCDSASVQSAAMATAPFEGNDPAAVFCAIRKLKNSF"
     gene            8756..9490
                     /locus_tag="PKW44_01070"
     CDS             8756..9490
                     /locus_tag="PKW44_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HQR50211.1"
                     /translation="MTTTYTDQDWLQTPLAQYLLEREQVLYDAVVADVFGFNAMQLGM
                     PQVDLLRDSRIPFRFRASPWGNVPVRCQFTQLPFATNSADLLLLPHVLEYSANPHDTL
                     REAERTLVPEGHLILSGFNPVSLWGMRRLFARQQSYPWSGRFLSLPRVKDWLALLGLE
                     VVAGRMGCYAPPFGKEKWIARCRFMDKAGDRWWPMLGGVYFLVAKKRVAGMRLIRPNW
                     NSSKVVQMLMPKPTQKTECQRTEHGK"
     gene            9480..9917
                     /gene="rnhA"
                     /locus_tag="PKW44_01075"
     CDS             9480..9917
                     /gene="rnhA"
                     /locus_tag="PKW44_01075"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HQR50212.1"
                     /translation="MENRVEIYADGACKGNPGPGGWGAWLSYAGKEKELCGGEPLTTN
                     NRMELLAVIRALEALKRGCKVRVHTDSQYVHKGISEWITGWKARGWRTADKKPVKNDD
                     LWRCLDELAQQHDIEWVWVKGHAGNVGNERADELANRGVEALK"
     gene            9914..10627
                     /gene="dnaQ"
                     /locus_tag="PKW44_01080"
     CDS             9914..10627
                     /gene="dnaQ"
                     /locus_tag="PKW44_01080"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HQR50213.1"
                     /translation="MRQIFLDTETTGLDPAQGHRIIEIAAVEVVNRRLTNNHFHVYLN
                     PDREIDPGAQEVHGITLEFLQDKPRFADTVDELLDFITGSELVIHNAPFDVGFLNAEL
                     GLLNRGRIQAACAGIVDTLKMAKELRPGQRNNLDALCRHYGIDNSARTLHGALLDAEL
                     LADVYLAMTRGQESLVMEMEVEKPGAEGLPAIRRSQPLVVLHADADELEAHRQYLSGL
                     DKAAGGIGLWNQSRTELKT"
     gene            complement(10646..12325)
                     /locus_tag="PKW44_01085"
     CDS             complement(10646..12325)
                     /locus_tag="PKW44_01085"
                     /inference="COORDINATES: protein motif:HMM:NF013109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HQR50214.1"
                     /translation="MLSRLWNSIKPFQGLLPIQRATALRDALAGFTLAAMNVPQALGY
                     TKIAGTPVISGFYTLLLPLVAFAALGSSRYLVVAADSATAAILATGLAGMAVTDTAHY
                     LSLASLVALLTAGLLLLARLLKLGFLSDFLSRTVLVGFLTGVGFQVGIAVLDELLRLP
                     MHTNQPLEQLLTIFRELPQTHWPTLAVSVCVITAILLLRRYAPKLPGALVAVVAAIAA
                     SASFDFARYGIEIIGPVTGGLPHLSLPPLDWPLVRSLLPIAGACFIMIMAQSAVTARA
                     YATRHHQALDSNRDLLGLSAANAAAACSGTFVVNGSPTQTAMVESSGGSSQLAHLSTA
                     LVVMLVLLLLTGPLQYLPVSALGAIVFTIAVHLVDIRGLDDLRQKSQDEWILALITAA
                     AVVVLGVEHGILLAIALSLLDHVRHGYRPNTAVLLRDPDDHWRMEPVAAGKHAEPGLI
                     VYWFGADLYYASANHFSEQVRHLVSDAPEPVKWLVIDAGAITGVDYSAGGVLRELHED
                     LEKQGVSLAFAHVNERLRNDLDQLGLTEAIGANHLFDTLRECVGAYREKSP"
     gene            complement(12354..12683)
                     /locus_tag="PKW44_01090"
     CDS             complement(12354..12683)
                     /locus_tag="PKW44_01090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR50215.1"
                     /translation="MSKTRMLALLSLGIVAAGCASDQQLLAGDQANAINVATRRGQFE
                     LSCPAATGTVLSSNLLQPALWGGIERAEYTVGVEGCGQKSTYVVVCQIGSVSCFAVSG
                     RNNAFTQ"
     gene            complement(12739..12972)
                     /locus_tag="PKW44_01095"
                     /pseudo
     CDS             complement(12739..12972)
                     /locus_tag="PKW44_01095"
                     /inference="COORDINATES: protein motif:HMM:NF012861.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(13004..13495)
                     /locus_tag="PKW44_01100"
     CDS             complement(13004..13495)
                     /locus_tag="PKW44_01100"
                     /inference="COORDINATES: protein motif:HMM:NF012861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HQR50216.1"
                     /translation="MLSHPDPDASKNVAKNRSGLLLLACAGLAVGALAGLAGSAFHWV
                     LDHADVLRDGVIAMAHATPWIGWLAPVGLASAAAFIARWLVRRYAPEASGSGVQYIET
                     VLQGENRPMRAAVLPVKFIGGVLAIGSGLALGREGPTVQMTRPSATGWHTGSGCGKAM
                     PRF"
     gene            13574..15061
                     /locus_tag="PKW44_01105"
     CDS             13574..15061
                     /locus_tag="PKW44_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HQR50217.1"
                     /translation="MTEPLLIAKSGKAELCLLPRMANRHGLVAGATGTGKSVTLQALA
                     EQFSHAGVPVFMADVKGDLAGMAMAGSANPKVEARVKELDLQGFGYAANPVVLWDVFG
                     EKGHPMRATISEMGPLLLARLLNLNETQAGVLTAVFKLADDNGWLLLDLKDLRALLQH
                     AAENAKELTGEYGNISAASVGAIQRGLLQLEQEGGEKLFGEPVLNFDDLMQTDNGRGV
                     INILAAETLYNAPRVYATLLLWLLSELFENLPEAGDLDKPKLVFFFDEAHLLFNDAPE
                     ALLQKIEQVVRLIRSKGVGVYFVSQNPLDIPDVVLGQLGNRVQHALRAFTPRDQKAVK
                     AAAETFRANPGVDVETVITELGVGEALVSFLDDKGRPVPVERALIRPPASRLGPLSDD
                     ERRTLIQSSILQGVYEKSVDRESAYEVLKARATQSAEQPPAEEGRGFDWGGLLGGQTG
                     PRGGRGREGVVEAAAKSVARAVGSELGRQIIRGVLGSILGGGKRR"
     gene            complement(15149..17929)
                     /gene="fdhF"
                     /locus_tag="PKW44_01110"
     CDS             complement(15149..17929)
                     /gene="fdhF"
                     /locus_tag="PKW44_01110"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018229404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HQR50218.1"
                     /translation="MNAPANTIPFNLNGARVEALEGETILQAARRNGTEIPHLCYTDG
                     LRPDGNCRACMVEIKGERVLAPSCCRAPAPDMEVSSDNARATAAQKMVLELLLSDMPP
                     KEYKLDNELTAWAEKMGVAHPRFDPRHSPDADLSNAAIAVNLDACIQCTRCVRACREI
                     EVNDVIGYAFRGSHAEIVFDQGDPMGDSTCVTCGECVQACPTGALSPGNGAAMVEPDK
                     SVDSGCPFCGVGCQLTYHVKDNKIISVTGRDGPANHNRLCVKGRYGFDYPAHKQRLTK
                     PLIRKPGVPKQAILDMDPANPYSAFREATWEEALDFAAAGLKKIIAGDGKYALAGFGS
                     AKGSNEEAYLFQKLVRTGFGTNNVDHCTRLCHASSVVALLEGVGSGAVSNPVSDVQFA
                     EVVIVIGSNPIVNHPVAATFIKNAVKKGTTKLILMDPRRTELARHAAYYLPFKPDTDV
                     AMLNAMLHVIVTEGLVDEEFIKNRTENYEALKENVKGFSPEAMAPICGIDARTLREVA
                     RLYATSKGSMILWGMGVSQHVHGTDNSRCLIALSMVTGQIGRRGTGLHPLRGQNNVQG
                     ASDVGLIPMVFPDYQRVDNPEIHAKFEKLWNTQLDNKPGLTVVEIMDAAYEGHIKGMY
                     VQGENPAMSDPNSNHAREGLARLEHLIVQDIFMTETAYLADVILPASSYAEKTGTFTN
                     TDRIVQMGRQAIDPPGDARQDLWIIQQIATRLGVNWNYDGPKEVFNEMRLAMQSIAGI
                     TWERLEQENAVTYPCEHEGDAGEEVIFVEDFPRPNGRARLVPADIIPADERPDSEYPM
                     VLITGRQLEHWHTGSMTRRASVLDAIEPAPTVSIHPLDMENIGVQAGETVTVESRRGE
                     VVLIARADDGIPPGSVFIPFCYYEAAANKLTNQALDPFAKIAEVKYCAVRVSKGGNLS
                     KELGYHTEPAVA"
     gene            complement(17926..>18432)
                     /locus_tag="PKW44_01115"
     CDS             complement(17926..>18432)
                     /locus_tag="PKW44_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018229405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HQR50219.1"
                     /translation="GLRSFSVSGRGKKPGGHLAPAGITMRELIDEYCGGMLDGHAFYG
                     YVPGGASGGILPAAMGDIPLDFDTLNQYGCFIGSAAVVVFSDRDKARELALNAMKFFE
                     DESCGQCTPCRVGTAKAAVLMGKPQWNKPLLEELSTAMMDASICGLGQAAPNPLRCVV
                     KYFPDELK"
BASE COUNT         3606 a         5201 c         5997 g         3628 t
ORIGIN      
        1 ttgcagaaat agcaggtaat cttcaggctg gaatcatttc gcgagcattc caagggctgg
       61 ctggccaagc tgatactggc attcatcacg gtcccgtttg ccttgtgggg gattgattcc
      121 tacctgcagg gggcgggctc caatgtcgcc gtggccaagg tgaacggaca aaacatcacg
      181 gtgcaggagt acgacaacgc gttgcagact ttgcgcagcc aattgcaaca ggaaggcaag
      241 actgacccgg cattgctgga tgatcctgca gtcaagcaat cggtgctgaa tcgtctgatt
      301 gtttcgcgtc tgctgacagg cgaggtgcgt agcgccaatc ttgtcatcag cgatgagcaa
      361 cttggtaaga ccatcttgga aatgccagaa ttttcacaga atggcaagtt ctcgcaggaa
      421 gtgtatgaca gtgttctgaa gcagaacaat ctgaccccga cacaatttga atcacgcatg
      481 cgtggtgagt tactgacaaa aaagctgcgt gacggcgtga cggctacggc atttgcaccg
      541 catgctgtgt cggacaacct gatccagata gatcgtcaac agcgcgaagt cagcattgcc
      601 gctatcaagg cagccgattt tcttgggcag gcgaaggtcg atgctgcgca agtccaggct
      661 ttttacgaca aaaacaagga taaattccgt gtccccgagc aggtcaagat cgagtttgtg
      721 atcttttcct caaacaatct gattacctct actccggttg ccgaagacga ggtgaagaag
      781 ttctaccaag aaaatgcttc caagttccag ggtgacgagc aacgtcgcgc cagtcatatc
      841 ctggttgctt ttgacaagaa ggatgatact gcaaaaaaag ctgcgcacga aaaggccgaa
      901 agtgtgctgg ctgaagtcaa caagaatccg gccaagtttg atgagttggc caggaagtat
      961 tcgcaggatc ccggctccgc tgccaatggc ggtgaccttg gcctgtttgg tcgcggcatg
     1021 atggtcaagc ccttcgagga tgccgtgttc agtatgaagc ctggtgcggt gagtggcctg
     1081 gtcgaatccg attttggcta tcacatcatc aagctgaccg agatcaaagg gcaggcgcca
     1141 acctacgatg acgtcaaagc acaaattcgt gccgaattgc tcaaccagaa ggcgctggcc
     1201 aagtttgccg agcaggcaga gggcttcaac aatatggtct atgagcagtc ggatagcttg
     1261 cagccggtgg tcaaggcatt caacgttccg ctacaaacca gccagtggat gagccgcgaa
     1321 gacgcgatga agtttttcaa gagcgacaag ctggtcaagg ccattttctc cgacgaagtg
     1381 ctgaaggata agcgcaatac ggaggcggtc gaggctgcgc cgaatacctt ggtggcggcc
     1441 cgcgtggtcg actacaagcc agctgcgccg cgcagctttg ccgaggttgc gcccgcgatt
     1501 gaagagcatc tcaagcgtga gcaggcgttt gccatggcag tcaagaaagg tgaggctgat
     1561 ctggcgcaat tgcgtgcagg cagggaagtg gctggcttgg agtggatccc gccagtggtg
     1621 atcgcccgcg ataacactca gggcttgagt gaaggtgtcg tgcagcaggc gttcaagatt
     1681 gatgctgaca agttgccggc atatggcagt ctgcaaaatc agggggctga ctacaccttg
     1741 attcgcgtca gccgggtgga atcttcggcg ccacaggacg aggctgaaaa gacgacattg
     1801 cggagtggga tacagacggc gttggctgct gaatatctgg ctgcctacat cggctccctc
     1861 aggtataagg ccaacatcac gaccaaccag cagttacttg gggcaggcgc gcagcagtaa
     1921 gcgtgatgca gcatatataa agacaaaagg ggcagaattc tgcccctttt gctttgatgc
     1981 gatgaagtag tttttattct accgtggccg cggccgtaat gttgaagcca ccgtccacat
     2041 aagtgatttc cccggtgacg ccgctggcca gatcgctgca caggaaagcc gagacgttgc
     2101 caacttcctc gatggacacg cttctgcgta gtgcggcatg tttgtcgttg aacgcgatca
     2161 ttttgtcgaa tcctgcgatg cctgaggctg ccagcgtctt gatcggacca gcggaaacgg
     2221 cgttgacgcg gatgcccctg gggccaaggc tttgcgccat atagcgtacg ttggcttcca
     2281 ggctggcctt ggctacgccc atcacgttgt agttgggcat ggtgcgctcg gcgccgagat
     2341 agctgatggt cagcagtgcg ccattgcgtc cctccatcat tggccggccg gctttggcca
     2401 gcgcggcgaa gctgtaggaa ctgacgtcgt gggcaatgcg gaaattgtcg cgcgtgacgc
     2461 tgtccaggta gtcgccggcc aaggcttccc taggcgcgaa ggcgacggaa tgcacgatga
     2521 tgtccagtcc atcccagcgc ttgccaagat tggaaaatag ggcatcgatc tgcgcgtcgt
     2581 ccgagacatc acaagggaac accaggtcgg agccgaattc tgccgccagt ttgccgacgc
     2641 ggtcctgaaa cttttccatc tggtaggtaa aggccagttc ggcaccttcg cgcctcatag
     2701 cctcagcgat gccgtaagcg atggagcggt tgctgagcag gccgacgatc agggcacgct
     2761 tgccagtgag aaatcccata gttattcctt tatttgtttg atctggggtc gagcgcgtcg
     2821 cgcaaccctt cgccgagaag attataaccc aacaccgtaa gcagaatagc cataccgggg
     2881 aacagtgaca gccaccaggc gaattcgatg tactccttgc cgtcggtcag gatgttgccc
     2941 caggaggcgt tcggtggctg tacgccgagg ccgaggaaac tcaggccgga ttccaccagc
     3001 accgcgctgg ccacgcccaa taccgtactg acaatgacgg gcgtcaggct gttgggtaac
     3061 aggtggcgga agatcaggcg gccatcccgc gcccccagga tttgcgaggc catgacaaat
     3121 tcgcgctcac gcaggctcag gaattctgcc cgtaccagcc gcgtcacccc catccaggag
     3181 gtgaggccga tgacgatcat gatgttccag atcgaggggg tcaggaaagc gatgacggcg
     3241 aggatgagga aaaaggtcgg gatggtcagc ataacgtcga ccaagcgcat gatgacggta
     3301 tccacccagc cccggtaaaa accagccagc gcgcccagga caatgccgat ggcagtggcg
     3361 atacccactg cgacaaagcc taccagcaga gaaatgcgcg cgccgaacag catgcgcgag
     3421 aacacgtcgc gccccaagcc gtcggtgccc atccagtggc tggaggaggg ggcgagcagg
     3481 atggccttga cgtcaatggc atcgggatca taaggcgcga ttagcggcgc cagcagcgcc
     3541 aacagcagta ccgtggcgat gatggcgaaa ccccagcggg cgagaggatt ggacagcgcc
     3601 tgtttcatcc gcccactccc cgccgcacgc gcgggtcggc ccaggcgtaa gctaggtcgg
     3661 ccagcaggtt gccgatcagg gtgagcgccg cgcctatggt gaggatgccc atgatgaccg
     3721 ggaagtcgcg catcagcacc gcgtcgtaga acagcttgcc catgccggga atggcgaaca
     3781 gcgattcggc gatgacggag ccgccgatca ggccggggat ggacaggccg aggatggtga
     3841 tcagtggcaa cagcgcgtta cgcagagcgt ggcgatagac cacggtgttt tccggcaggc
     3901 ctttggcgcg cgccgtggtg atgtagtcct ggtgcagcac gtccagcatg ccgttgcgca
     3961 cgaacagtga aatgccggcc agaccaccca gtgccgagat gaataccgga atggcgagat
     4021 gcctggctgt gtcccaccac cgcgctggcc aagtcaggtg ttcatagccc aagctgtgca
     4081 gcccggaaat cggcagccag tcatgcgcca cgcctagcca gtacatcatc atcagcgcca
     4141 gccagaagcc tggcacggca aagccgatga agacgaatac cgtgatcgtc cgatccggtg
     4201 gtttgtttgg attgagcgcg gaaattacgc ccagcggcag cgcgacggcg atgatcagta
     4261 ccaggctgag cacgttgatc agcagcgtga tgggcagcgc ttcctgaatc atgcccttgc
     4321 tgacattgcc tttgtcgtcc ttgcgttcga ggaataccgg gcgctggtgg ctggcaaacg
     4381 aggtaccgaa atccagcgtc gccatgcgct tcagccacag gccatattga acaatcaatg
     4441 gctcgtccag atggtacagc tggcgcagct tttggcgaga ttcctggctg gctttggggt
     4501 tgaagccagc ttcggtggtg gtgatgtcgc ccggcgcaag gtgcatgatg aggaacgaga
     4561 tcaggctgat gccgaccagt agcggcacca tccagaacag gcgtctgagc aggtagcgtg
     4621 tcattgggcg gaaatctcgg tacggcgcaa cggctcggga atgtaccact cgtaggcgtt
     4681 atggccgatg ccggccggcg gcgccgggtt gtcgatgccc ttgacgcgct tgtggatggc
     4741 gggcaggcca tagccggcgt acaagtagac gatggggctg tcttccagca ggattttggc
     4801 gaactggtgg taaatttcct gacgcttgtc cgggttcagt tccagccgtc cctgttccag
     4861 cagcttgtcc acctcggggt tgctgtagcc gatgaagttg aactggcccg gcgcttgttg
     4921 cgaggagtgc cagatgttga actggtccgg ttcctgccct agcccccagc ccagcaggac
     4981 cacatcgaaa tcgccggtct tgatgaagcg gccgatgaag ctggcccatt ccagcacgcg
     5041 aattttgacg tcgatgccga tgtccttgag acggcgctgg atcagggtcg cgctcatctc
     5101 ccgctgcttg ttctggttgg tgaggatctc gaacgccagc ggcttgccgt ctttgtccag
     5161 aatgccgtca ccgtcatggt cggcgtatcc ggcgtccttc agtagttgtt tggctttttc
     5221 cgggtcgtag ggataagggt gcaacctggg gttgctccag cgtgtgcccg gcttataggg
     5281 ggaggctacc ggctctccca gccccagcaa tacgccgtct atgatttcct gcttgtcgat
     5341 ggcgtagttg atggcctggc gtacacgctg gtcgtcaaat ggcttgtgct tgagattgaa
     5401 gccgaggtag gtgtaggcgt tgcccagttc cttgtacagg gcgatcttct ggttcagttc
     5461 ggggcgggcg gggaagatgc gtgcgtactg gattgggctg aggcccatgc tgtcgatatt
     5521 gtcagccatc agctccagaa actgggccgc cgggtcgggg atgaagcgcg acaaaagccg
     5581 gtcgatgcgg gcttggccct gggtggcgtt gggattgcgt accagtgaga tgcgctcgcc
     5641 gttggtccat tctgccagct ggtagtagtg gctgccaacg ggatggcggg caaaggcggt
     5701 ggtgttaatg tcctgacctt gcggcagatg cttgggcagg atttgcaggc cggcccagga
     5761 atccagcgcg ggcgcataag gctgggcata agaaacacgg aatgtctgtg cgtccggcgc
     5821 ctctgccctg atgaccagct tgtagtcggc gccatagggg gtacgcgtat tgtcgtcggt
     5881 gactagtttc caggtgaaca gcgcgtctgc actggtcaag ggtgcgccgt ccgcccactt
     5941 gagatcgggt ttgaggtgga aagtaatggt tttctggtcg tcggagatgt cccaggactt
     6001 ggccagttcg ccttccagtt ccaggttctt gtcgtacttg agcagggtgt tgaagatatt
     6061 ggcggcgatg gcggaggcgg ccgattcccc ggctagcatc gggatcaggc cgctgggctc
     6121 gccggccatg gcatccacga gtgtgccgcc cgattgcggc ggatagggac gattgagatc
     6181 ggcgtttgaa ctgtcagcgg gccgcccgca tgcggcgagc aggaaaaatg ccgccagaaa
     6241 cggcaacagc ttacgcggca tccggcttga agattgtcag tttatgtcct ggaaccagtc
     6301 ggtcgccact cagcctgttc cagcgctgca gatcgttggt ggcgacatcg aatttgcgtg
     6361 caatttctgt cagggtgtcg ccacggcgaa cgacgtagtg aattttttcc ggcgcgctgc
     6421 tggccctggt tcgtactgta gattcgttgg caatgtctgc ccgtaccagc aatacctggc
     6481 cgctacgcag cttgcgagat ttaagatggt tgagcgcgac caggctgttg gcggacatgt
     6541 catagcgctt ggcaacgctg ttgacgctat ctcctttttt cacgacatgg tgcttcgtat
     6601 ccggggttgc gccagcttgt tcgggggcct gcgtggcagt ttctgctacc gataatgcgg
     6661 attcgccgcc cgtttgggcg ggtaccagca gaatgctggg ctggctgagc ttgccgcctt
     6721 tgccgacaat atcgttgaca tcgcgtagtt gcgccaggct gatgccaaac ttgcgggcaa
     6781 tggcgtcctg acgctcgccg cgcttggcgt tataggtttg ccagctcacc agaggctggt
     6841 tgtaagcggc gaggttggcg ctgaattcct cggccttacc taccggcagc aaaaattcat
     6901 gcgtgggtcc gctggaggtc agcaccggac ggttgtattc ggggttgagc gccaggaatt
     6961 cttcctgaga tatcccggca agcttggcgg ccaggctggc atcgatttgc ttgggcgcct
     7021 gtactgtcgt gaagtaaggc tggttggcga tgggccggat atccaggcca tactgctcgg
     7081 ggttggtgac aatgttcttg accgcttgca acttgggtac gtaattcttg gtttccgctg
     7141 gcaggctgag gctttggtag tcggtcggca agccctgctt gcggttgcgc tcgatggcgc
     7201 gcattaccgt gccctcgccg gcgttgtagg ctgccagcgc caagtcccaa ctgccgaaca
     7261 tcacgtgaag cttctgcaag taatcaagcg ccgcatcggt ggccgccatc acgtcgcggc
     7321 ggttgtccac ccaccagtcc tgtttgaggc cgaagtgctt gccggtagag ggaatgaatt
     7381 gccagatgcc agaagcgcgg ctggtggaat tggcctgcgg gttgaatgcg ctttccacca
     7441 tgggcagcag ggcgatctcg gtcggcatgc cgcgcttttg cacttcctcg acgatgtggt
     7501 agagatactt ctggctacgg ccgaccatgc gcctgacata gtcgggccgc gcggcgtacc
     7561 aggattcatg cgtcgcagtg aaaggggagc gcagttccgg catggcgtaa ccgcccttga
     7621 tgcgttgcca caggtcgttg ctgttcgcct gggtcggggc ggctgcgcca ttttcaccgc
     7681 tgtcttgtac cagcggtggc agttgctgca cgctatccgg ggcttgtggc agggattcgt
     7741 agttgaacag ttcgctgtcg cgacgtccgt acacctgggc atcgctgtaa taggtgattt
     7801 catcgccggc ggcgttggtg tcggcgtgtg cgggcgcgag gtagggtagg gacagaataa
     7861 ccagttgcag caggtgtttt acagccatgt cgcggttccc tggcaggcaa aaattattgc
     7921 aatgctatcc cctaacaagg gctgccgtca aggtgtgaat tggctgaaat tctgatagca
     7981 actaaatgat tagaatgaat ttttaagctt cctgatggca cagaacaccg ctgccggatc
     8041 attgccttcg aatggggctg tcgccatggc cgcagactga acgctggcgg agtcgcagcg
     8101 cagaaaggga ttggtggcgc attccagcgc taaagaagaa ggcaggctgg ggcgtccatc
     8161 agccctgcaa cgctcgacat cacgcttgcg cagcgcgaga tccgcattgc cgggatccac
     8221 ggccagcgca aaccggatgt tgtccagtgt gtactcatga gcgcagtaga cccgggtttg
     8281 cagtggcaac gcggccaacc gctgcagtga ggcgtagagt tgctcgcaac tgccttcgaa
     8341 caggcgtccg cagccgcagg agaacagggt gtcgccacag aacagcgaat ttgcgccata
     8401 ataagccacg tggtccagcg tgtggccggg cgtttcaagg acttgcaggc agatgtccag
     8461 ggtggtcagg tcaatgcagt cgccttcgcc aaccggctga tgcgggaaag cgtagtggcc
     8521 tttgcgcgga gcataaacag atatcgggaa tgtgcgaacc agctcggtga caccgccgat
     8581 gtggtcgtca tggtggtgcg tgaccagaat ggcgtcgagc tggagttgtt gctcggcgag
     8641 cagcgccagg actggcgccg catcccccgg gtcgaccacc acggcatggc ccttccgccg
     8701 caacagccag atatagttat cccggaaagc cgggatgggg atcacttcca gcatcatgac
     8761 aacaacatac accgatcagg actggctgca aaccccgcta gcgcagtatc tgctggagcg
     8821 ggagcaggtg ctctatgatg ccgtggtagc cgatgttttt ggcttcaacg ccatgcagct
     8881 gggcatgccg caagttgatc tgcttcgcga cagccgcatc ccgttccgct ttcgcgcctc
     8941 gccttggggg aatgtgccgg tgcggtgcca gttcacgcaa ctgccttttg ccaccaatag
     9001 cgcggatttg ctgctgctgc cgcatgtact ggaatattcc gccaatccgc atgacacgct
     9061 gcgcgaagcc gagcgcaccc tcgtgccgga aggacatttg atactcagcg gattcaatcc
     9121 ggtcagcttg tggggcatgc gccgcctgtt tgccaggcag caatcctatc cgtggtccgg
     9181 ccgttttctg tctttgccga gagtcaagga ctggttggcg ctgcttggcc tggaagtggt
     9241 ggccgggcgc atgggatgct atgcgccccc tttcggcaag gaaaagtgga tcgcccgctg
     9301 ccgcttcatg gataaggccg gagaccgctg gtggccaatg ctgggcgggg tttattttct
     9361 ggtggcaaag aagcgggtgg ccggcatgcg cctgatccgg ccaaactgga atagctcgaa
     9421 agtggtgcag atgctcatgc ccaagcccac acaaaaaacg gaatgtcaga gaaccgagca
     9481 tggaaaatag ggtagaaatc tacgcggatg gcgcctgcaa gggcaacccc ggccccggcg
     9541 gctggggtgc ctggctgagc tatgctggca aggagaagga gttgtgcggc ggtgaaccgc
     9601 tgaccaccaa caaccgcatg gaattgctcg cggtcatccg cgcgctggag gcgcttaagc
     9661 gcggctgcaa ggtgcgtgtg cacaccgatt cgcaatacgt ccacaaaggc atcagcgagt
     9721 ggatcactgg ctggaaggcg cgcggctggc gaaccgccga caagaaaccg gtcaagaacg
     9781 acgacctctg gcgctgcctg gacgaactgg cgcaacagca tgacatcgaa tgggtgtggg
     9841 tcaagggaca tgccggcaac gtgggcaatg aacgggcgga tgaattggcg aatcgcggag
     9901 tagaagcact gaaatgagac agatttttct cgataccgaa accaccggcc tcgatccggc
     9961 gcaggggcac cgcatcatcg aaatcgcggc ggtggaagtg gtgaaccgcc gcctgaccaa
    10021 caaccatttc catgtctacc tcaacccgga tcgggagatc gaccccggtg cgcaggaggt
    10081 gcatggcatc acgctggagt ttctgcagga caagccgcgt tttgccgaca ccgtggacga
    10141 gcttctggat ttcatcaccg ggtcggagct ggtgatccac aatgccccgt tcgatgtcgg
    10201 tttcctcaat gccgagctgg gtttgctgaa cagggggcgt attcaggcgg cctgtgccgg
    10261 catcgtcgat acgctcaaga tggcgaagga gctgcgcccg gggcagcgca acaacctcga
    10321 cgccctgtgc cgtcactacg gcatcgacaa ttccgcgcgc accctgcacg gcgccttgct
    10381 ggatgccgag ctgctggctg acgtctacct cgccatgacg cgcggccagg aaagtctggt
    10441 gatggaaatg gaggtcgaga agccaggagc cgagggcctg ccggctatcc ggcgtagcca
    10501 gccgctggtg gtacttcatg ccgacgccga tgaacttgag gcgcatcggc aatacctgag
    10561 tggtctggac aaggcggcgg gtggcatcgg cctgtggaac caatcccgga cagagctcaa
    10621 gacctgaaca acttgtctag gcaggctagg gcgatttttc ccgataggcg ccaacgcact
    10681 cgcgtagcgt gtcgaacaga tggtttgcgc ctattgcttc ggtcaggccc agttggtcga
    10741 gatcgtttct caggcgctcg ttgacgtggg caaatgccag actgacaccc tgtttttcca
    10801 ggtcttcgtg cagttctctc agcaccccgc ctgctgagta gtccacgccg gtaatcgcgc
    10861 cggcgtcgat caccagccac ttgaccggct ccggcgcatc gctgaccaga tggcgtacct
    10921 gttcggagaa gtggttggcg ctggcgtagt agaggtcggc gccgaaccag taaacgatca
    10981 ggccgggttc ggcatgcttg ccggcagcca ccggctccat gcgccagtgg tcgtccgggt
    11041 cgcgcagcag cactgcggtg ttcggccggt agccgtggcg cacgtggtcg agcagcgaga
    11101 gcgcgatggc cagcaggatg ccatgctcga cccccagcac gacgacggca gcggcagtga
    11161 tcagggcaag aatccattcg tcctgcgact tctgccgcag gtcgtccagc cccctgatgt
    11221 cgacgaggtg cactgcaatg gtgaatacga ttgcgccgag cgcgctgacc ggcaggtact
    11281 gtagcggacc ggtgagcagg agcaggacca gcatcaccac cagtgcggta gacagatgcg
    11341 ccagctgact cgagccgccg gagctttcca ccattgctgt ctgtgtcgga ctgccattga
    11401 ctacgaaggt gccgctgcag gcggcggcgg cattggccgc cgacaggccg agcaggtcac
    11461 ggttggaatc gagggcctga tggtgccgcg tggcataggc gcgggcagtg acggcacttt
    11521 gcgccatgat catgatgaaa cacgcgccag cgatcggcag cagggatctg accaatggcc
    11581 agtcaagcgg tggcagggaa agatgcggca ggccgccggt cacgggcccg ataatctcga
    11641 tgccgtagcg cgcgaaatcg aagctggcgc tggcggcaat cgcagcgacg acagcgacca
    11701 gtgcgccggg cagtttgggg gcatagcgcc gcaacagcag gatagcggtg atgacgcaga
    11761 ccgagaccgc caaggtcggc cagtgggttt gcggcagctc cctgaagatg gtcagcagtt
    11821 gttcgagcgg ctgattggta tgcataggca aacgcagcaa ctcgtcgagc acagcgattc
    11881 caacctggaa tcccacgccg gtcaggaagc cgacgaggac ggtgcgggac aggaaatcgg
    11941 acaggaatcc caacttcagc agccgtgcca acagcagcaa accggctgtg agcaatgcca
    12001 ccaggctggc caaagacaga tagtgtgcgg tatccgtgac cgccattcct gccaggccag
    12061 ttgcgaggat cgccgccgta gccgagtcgg ccgccactac cagatagcgt gacgagccta
    12121 gcgcggcaaa tgcgaccagt ggcagcagca gagtatagaa gccgctgatg actggcgtgc
    12181 cagcgatctt ggtataaccc agggcctgcg ggacattcat tgccgccaga gtgaatcccg
    12241 ccagcgcatc acgtaatgcc gtggcgcgct ggatcggcag caagccctgg aatggcttga
    12301 tgctgttcca gaggcgggaa agcatcggtg gcagcatcaa aggaagggaa tgcttattgc
    12361 gtaaacgcgt tgttgcggcc ggagacggcg aaacagctga ccgagccgat ctggcagacc
    12421 acgacatagg tggacttctg tccgcaaccc tcgacgccga cggtgtattc ggcacgctcg
    12481 atgcctcccc acagtgccgg ttgcagcagg ttgctggaga gtaccgtgcc ggttgcggcg
    12541 gggcaggata gttcgaactg gccgcggcgc gtggcgacgt tgatggcatt ggcctggtcg
    12601 ccggccagca gttgctgatc gctggcgcag ccggccgcga cgatacccag gctcagcaag
    12661 gccagcattc tggttttcga catgacaatc tccttattca gttaaaaggt attgcggatt
    12721 attagacgct tcgtggcttt atgcaaggca ggcattttca gctgtatcgc gctcccgcag
    12781 cgtgtcatag atcggccgga cgccaagcag gttcggcacg gccaaggcgg tgagcaaagg
    12841 aatgaacagc gctgtggcac cggtcatctc gaccaccagc gccatgccgg tcaacggggc
    12901 gccgaccacg cgcacgttga agcgtcgcag caattcctca aatacgaaaa tcgcgccggc
    12961 gagcggggca ttgaaggcgg cgccagcccg actccggctg cgatcagaac cttggcatcg
    13021 ccttcccgca gcctgagccg gtgtgccagc ccgtggccga aggtcgcgtc atctgtaccg
    13081 tcggcccctc gcggccaagg gcgaggccgg aaccgatggc cagcacgccg ccgataaact
    13141 tgactggcag aacggccgcg cgcatcgggc gattctcccc ttgcagcact gtttcgatgt
    13201 actgtacgcc gctgcccgaa gcttcggggg catagcgcct taccagccag cgcgcaataa
    13261 aggcggcagc ggaggcgagt ccgaccggcg ccagccagcc gatccagggt gtggcatggg
    13321 ccatggcgat gacgccgtcg cgcagtacgt ctgcgtggtc gagaacccaa tgaaatgcgc
    13381 tgccggccag cccggcaagt gcgccgacag ccaggccggc acaggcgagc agcaataacc
    13441 cagagcggtt ctttgccaca ttcttggagg cgtctgggtc ggggtggctc agcataagca
    13501 agctccggga actcaaattt tttcgatcac gggttatgat accggcatcc gcttacttgc
    13561 caaggaacaa atcatgacgg aaccgctttt gatcgccaaa tccggcaagg ccgagctttg
    13621 cctgctacct cgcatggcca accgccacgg cctggtggcc ggtgccaccg gtaccggcaa
    13681 gagcgtgacc ctgcaggcac tggccgaaca gttctcccac gctggcgtgc cggtgttcat
    13741 ggctgacgtc aagggcgatc tcgccggcat ggccatggcc ggaagcgcca accccaaggt
    13801 ggaagcgcgg gtcaaggaac tcgacttgca agggtttggc tatgccgcca acccagtggt
    13861 actgtgggac gtgttcggcg agaaaggcca tcccatgcgt gctaccatct ccgagatggg
    13921 gccgctgctg ctggcgcgcc tgctgaatct caacgagacc caggccgggg tgctgaccgc
    13981 cgtgttcaaa ttggcggacg acaacggctg gctgctgctg gacctcaagg atttgcgcgc
    14041 gctgttgcag catgcagcgg agaatgccaa ggaactgacc ggggagtacg gcaatatttc
    14101 cgccgccagc gtgggcgcca tccagcgcgg gctgttgcag ctggagcagg agggcggtga
    14161 gaagctgttc ggcgagccgg tgctcaattt cgacgacctg atgcagaccg acaacgggcg
    14221 tggcgtcatc aacatcctgg cggcggaaac cctgtacaac gcgccgcgcg tctatgccac
    14281 gctactgctg tggctgcttt cagagctttt cgagaacctg ccggaagcgg gcgacctcga
    14341 caagccgaaa ctggtgttct tcttcgacga ggcgcacctg ctgttcaacg atgcaccgga
    14401 agccctgctg cagaagatcg agcaggtggt gcggctgatc cgctccaagg gcgtaggggt
    14461 gtatttcgtc agccagaatc cgctggacat cccggacgtg gtgctgggac agctcggcaa
    14521 ccgcgtgcag cacgccctgc gcgctttcac cccgcgtgac cagaaggcgg tcaaggctgc
    14581 cgccgagact ttccgcgcca atcccggcgt ggatgtggaa accgtgatta ccgaactggg
    14641 tgttggcgaa gcgctggtct ccttcctcga cgacaagggc aggccggtgc cagtggagcg
    14701 tgccctgatc cgcccgccgg ccagccgtct cggcccgctg agcgatgatg agcgtcgaac
    14761 gctgatccag tcctcgatcc tgcaaggggt ttacgaaaaa tcagtggacc gcgaatcggc
    14821 ctacgaggtg ctcaaggcgc gcgccacgca atccgcagaa cagccgccgg cggaagaggg
    14881 cagggggttc gactggggcg gtctgctggg tggccagacc ggaccgcgag gcggcagggg
    14941 gcgggaaggg gtggtggaag cggcggccaa gagcgtggcg cgcgcagtcg gctcggaact
    15001 cggccgccag atcatccgcg gcgtgctggg ctccatcctg ggcggaggga agcgccgcta
    15061 aggccggccg gacccggact gaatcaatac gatggctttg aatgtaaccg ggcggcgact
    15121 tgcccggtac gacaatagcc cggggcggtt acgcgacagc cggttccgtg tggtagccga
    15181 gttctttcga cagattgccg cccttgctta cccgcaccgc gcagtacttg acctccgcga
    15241 tcttggcgaa cggatccagc gcctggttgg tgagtttgtt ggcggcggct tcgtaatagc
    15301 agaacggaat gaacaccgag ccgggcggaa tgccatcgtc ggcacgagca atcaggacca
    15361 cctcgccacg gcgtgattcc accgtgacgg tctcgccagc ctgcacaccg atgttttcca
    15421 tgtccagcgg atggatgctg accgtgggcg ccggttcgat ggcatccagc accgaggcgc
    15481 ggcgcgtcat ggagccggtg tgccagtgtt ccagctggcg cccggtgatg agtaccatgg
    15541 ggtattcgct gtccgggcgc tcgtcggccg ggatgatgtc ggcgggcacc agcctggcgc
    15601 ggccgttggg gcgcgggaaa tcttcgacga aaatgacttc ctcgcccgca tcgccttcgt
    15661 gctcgcacgg gtaggtgacg gcattttcct gctccagccg ctcccaagtg atgccggcga
    15721 tgctctgcat ggccaggcgc atctcgttga atacttcctt cgggccatcg tagttccagt
    15781 tgacgccaag acgggtggcg atctgctgaa tgatccacaa gtcctggcgc gcatcgcccg
    15841 gcgggtcgat cgcctggcgt cccatctgca cgatgcggtc ggtattggtg aaggtgccgg
    15901 tcttttcggc gtaggaggat gccggcagga tgacgtcggc caggtaggca gtctcggtca
    15961 tgaagatatc ctgcacgatc aggtgctcca gccgagccaa tccctcccga gcatggttgc
    16021 tgttaggatc cgacatggcc ggattttcac cctggacgta catgcccttg atgtgtcctt
    16081 cataggcggc gtccatgatt tctaccacgg tgaggccggg cttgttgtcc agttgggtat
    16141 tccacagttt ttcgaatttg gcgtgaattt ccgggttgtc cacgcgctgg tagtccggga
    16201 acaccatggg gatgaggccg acgtcggacg ctccttgtac gttgttctgc ccgcgtagcg
    16261 ggtgcaggcc ggtgccgcgc ctgccgatct gcccggtgac catggacagg gcgatcaggc
    16321 agcgggaatt gtcggtgccg tgcacgtgct gcgacacgcc catgccccac agaatcatgg
    16381 aacccttgga ggtggcgtac agacgggcga cttcgcgcag cgtcctggca tcgatgccgc
    16441 agatcggcgc catggcttcc ggcgagaagc ccttgacgtt ttctttcaaa gcctcgtaat
    16501 tttcggtgcg gttcttgatg aattcctcgt ctaccaggcc ttcggtaacg atgacatgca
    16561 gcatggcgtt gagcatggcg acatcggtat cgggtttgaa tggcagatag taggctgcat
    16621 ggcgcgccag ctcggtgcgg cgcggatcca tcaggatcag cttggtggta cctttcttga
    16681 ccgcattttt gatgaaagtg gcggcgaccg ggtggttgac gatggggttg gagccgatga
    16741 cgatgacgac ttctgcgaac tgcacgtccg ataccggatt ggacactgcg ccggaaccca
    16801 cgccctccag cagcgccacc accgatgagg cgtggcacag gcgggtgcag tggtcgacgt
    16861 tgttggtgcc gaagccagtg cgcaccagct tctggaacag ataggcctcc tcgttgctgc
    16921 ctttggctga accgaaaccg gccagcgcat acttgccatc cccggcgatg attttcttca
    16981 ggccggcggc ggcgaagtcc agtgcctctt cccatgtggc ttcgcggaat gccgagtaag
    17041 ggttggctgg atccatgtcc agaatggcct gcttgggtac gcccggcttg cggatcagcg
    17101 gtttggtcag gcgctgcttg tgtgccgggt agtcgaagcc gtagcggccc ttgacgcaca
    17161 ggcggttgtg gttagccggg ccatcacgtc cggtgacgct gatgatttta ttgtccttga
    17221 cgtgataggt gagctgacag ccgacgccgc agaacgggca accggaatcc acgctcttgt
    17281 ccggctcgac catggcggca ccgttgcccg gtgacagcgc cccggtaggg caggcctgta
    17341 cgcattcgcc gcaggtgacg caggtcgagt cgcccatagg atcgccctgg tcgaacacga
    17401 tttccgcatg gctgccgcgg aaggcgtagc cgatcacgtc gttgacctcg atttcgcggc
    17461 aggcacgcac gcagcgcgta cactggatgc aggcatcgag attgacggcg atggcagcgt
    17521 tggagaggtc ggcgtccggc gaatgacgcg ggtcgaagcg cggatgggcg acccccatct
    17581 tttcggccca ggcggtgagt tcgttgtcga gcttgtattc cttggggggc atgtcggaaa
    17641 gcagcagttc cagcaccatt ttctgcgcgg cggtggcgcg ggcattgtcc gagctgactt
    17701 ccatgtctgg tgccggggcg cggcagcagg atggcgccag cacccgctcg cccttgattt
    17761 cgaccatgca ggcgcggcag ttgccgtccg gccgcaggcc gtcggtgtag cacaggtggg
    17821 ggatttcggt gccgttgcgg cgcgccgcct gcaggatggt ttcgccctcc agcgcttcga
    17881 ctcttgcgcc gttcaggttg aagggtatgg tatttgcagg tgcgttcatt tgagctcgtc
    17941 cggaaaatac ttgaccacgc agcgcagcgg gttaggtgcc gcctgcccca gaccgcagat
    18001 ggatgcatcc atcatagccg tggagagttc ttccaacaat ggcttgttcc attgtggttt
    18061 tcccatcagg acagcggcct tggcggtgcc gacgcggcag ggcgtgcact gcccgcagga
    18121 ctcgtcctcg aagaacttca tggcgttgag tgcgagttcg cgtgccttgt cgcggtcgga
    18181 gaacacgact acggcggcgg aaccgatgaa gcagccatac tggttgagcg tgtcgaaatc
    18241 gagtggaata tcacccatag ccgctggcag aatgccgccc gaggcgccgc cggggacgta
    18301 gccgtagaac gcgtggccgt ccagcatgcc gccgcaatat tcgtctatca gttcgcgcat
    18361 ggtgatgccg gccggcgcca gatgtccccc cggcttcttc ccccggccgc tgaccgagaa
    18421 cgagcgcagg cc
//
LOCUS       DAOMFA010000010        18127 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_217_length_18127_cov_5.381308, whole genome shotgun sequence.
ACCESSION   DAOMFA010000010 DAOMFA010000000
VERSION     DAOMFA010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 18127)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18127)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18127
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_217_length_18127_cov_5.381308"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            176..1585
                     /gene="glnA"
                     /locus_tag="PKW44_01120"
     CDS             176..1585
                     /gene="glnA"
                     /locus_tag="PKW44_01120"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HQR50220.1"
                     /translation="MAVADVLKMVKDNEIRFVDFRFTDTRGKEQHVSVPVSAFSEDKF
                     TEGHAFDGSSIAGWKGIQASDMLLMPDPDTAFVDPFFDEPTLVLSCDVVDPTDGRGYD
                     RDPRSIAKRAEAYLKSSGLGDTAYFGPEPEFFIFDSVQWEVGMSGCHVKITSEEAAWT
                     SGESLEGGNIGHRPGIKGGYFPVPPVDSLHDIRSAMVMALEEIGIPVEVHHHEVATAG
                     QCEIGTVFNTLVKRADWTQQLKYIVHNVAHQYGKTATFMPKPIVGDNGSGMHVHQSVW
                     KDGKNLFAGNGYAGLSEFALYYIGGIIKHVKALNAITNPGTNSYKRLVPHYEAPVKVA
                     YSAKNRSASIRVPHVQSDKARRIETRFPDPIANPYLAFSALLMAGLDGVQNKIHPGDP
                     ADKNLYDLPPEEDAKIPTVCASLDEALDALDKDREFLTRGGVFSNEWIDAYIGLKMEE
                     VNKIRMTTHPIEFQMYYSC"
     gene            1703..2179
                     /locus_tag="PKW44_01125"
     CDS             1703..2179
                     /locus_tag="PKW44_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HQR50221.1"
                     /translation="MKKLLFILIGLGIQLGAHAEIYKQVDADGHVTYSNVPIKGATKL
                     NLDPPPSGSSSTKAPARTATPANFPRVDQETQRQRDDKRRQILEDELANEKKALEEAR
                     KNYVEGEANPEMFTAAGGKRFRNVPKYEEKMKGLQEDVSQHEKNVELLEKELGSLK"
     gene            2229..3326
                     /gene="glnL"
                     /locus_tag="PKW44_01130"
     CDS             2229..3326
                     /gene="glnL"
                     /locus_tag="PKW44_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HQR50222.1"
                     /translation="MTHSVPSTFSGLEHLSTAVLLLDDQGFVLYTNPAAELLFAVSAN
                     QINGHTLAQVFPGCDILLAAVTSSLASNSPFREHEFLITTLRHHSFAATCTATPVELA
                     PARLVLEFQQMDQQLRIAREERMLIQQQANAELLRNLAHEIRNPLGGLRGAAQLLEHE
                     LPQPGLREYTQVIIKEADRLQQLMDRLLVPHRVPKYEPTNIHEVLERVRSLLLAESPQ
                     GVSIWRDYDTSLPDLIGDREKLIQAVLNIARNAVQAMQGQGEIVFRTRAQRQVSLAKK
                     RYRVAIKLQIIDNGPGIPKEIRDRIFYPLVSGRAEGSGLGLTLAQTFITQHHGMIDCE
                     SEPGRTCFTILLPLEATAYKPTHLSEQAKAP"
     gene            3323..4711
                     /gene="ntrC"
                     /locus_tag="PKW44_01135"
     CDS             3323..4711
                     /gene="ntrC"
                     /locus_tag="PKW44_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HQR50223.1"
                     /translation="MKPIWIIDDDRSIRWVFEKALARTDMEFRTFASVPEAMDALADD
                     EPQVVVSDIRMPNGSGLDFLQEIKRRLPDIPVIIMTAYSDLESAVAAFQGGAFEYLAK
                     PFDVDQAVDIIRRAVDESMRQATESEEPQETPEIIGQAPAMQEVFRAIGRLSRSHATV
                     LINGESGTGKELVALALHRHSPRSEKPFIAINTAAIPKDLLESELFGHERGAFTGAQA
                     ARRGRFEQADGGTLFLDEIGDMPPDLQTRLLRVLSDGQFYRVGGHQPVKVNVRIIAAT
                     HQDLEDRVRQGLFREDLFHRLNVFPLRLPTLRERREDIPLLARHFLQQSALELGVEPK
                     QLAPAALRYLSGLGWSGNVRQLENVCHWLTVMAPGQNIDIADLPPEAREEHGRPANTA
                     SWQEALAQEVADALSRGEQNILEARAQEFERIMIRKALEHTGGRRIEAATQLGMGRNT
                     LTRKIQELEIED"
     gene            complement(4724..5659)
                     /gene="lipA"
                     /locus_tag="PKW44_01140"
     CDS             complement(4724..5659)
                     /gene="lipA"
                     /locus_tag="PKW44_01140"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HQR50224.1"
                     /translation="MDNAQTKQKGAAKTARIPIKIVPQPALRKPEWIRMKVPDGPRFQ
                     EIKQILRDNSLHTVCEEASCPNIGECFSGGTATFMILGDLCTRRCPFCDVAHGKPLPP
                     DAEEPENLARTIAAMRLKYVVITSVDRDDLRDGGARHFADCIRIARAYSPHLRIEVLV
                     PDFRGRLDVALELLAEAPPDVMNHNLETVPRLYKQARPGADYAHSLKLLANFKARFPG
                     IPTKSGLMLGLGETDEEIFEVMRDLRGHGVEMLTLGQYLQPSAHHLPVLRFVEPQRFQ
                     WFERQALAMGFTNAACGPMVRSSYHADMQAHEAGV"
     gene            complement(5665..6117)
                     /locus_tag="PKW44_01145"
     CDS             complement(5665..6117)
                     /locus_tag="PKW44_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018506572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3465 domain-containing protein"
                     /protein_id="HQR50225.1"
                     /translation="MKKLLLIAILVVAYFQFAQTHETSSSPASLFSNQSEVAAASDQP
                     IAVAFEQRQSNVQVEGGGTVSRILGDDVNGSRHQRFIVRLANGQTLLLAHNIDLAPRV
                     DGLSEGDRVSFYGEYEWNAKGGVIHWTHHDPAGQHAAGWVRHNGKTYQ"
     gene            complement(6128..6730)
                     /gene="lipB"
                     /locus_tag="PKW44_01150"
     CDS             complement(6128..6730)
                     /gene="lipB"
                     /locus_tag="PKW44_01150"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HQR50226.1"
                     /translation="MLARHLGLTDYEATWRAMQDFTAQRQADTPDELWLTEHPPVYTL
                     GLNRKDVRLPWRDDIPLVLTDRGGKITYHGPGQIVLYLLLDLKRSGLKIRQLVDCMEG
                     AIINLLAEYGITSQGRPDAPGVYVEGKKIASLGLRLKNGCCYHGLSLNVDMDLSPFEA
                     IDPCGYRGLQVTQMRDLGIIHGSGEYASRLTAAMMKSLES"
     gene            complement(6733..6999)
                     /locus_tag="PKW44_01155"
     CDS             complement(6733..6999)
                     /locus_tag="PKW44_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 domain-containing protein"
                     /protein_id="HQR50227.1"
                     /translation="MADQETLLEFPCEFPLKVMGETRDGFVEAVVAVVRGHDPAFDAT
                     CVEMRASSGGKYMSLTCTINATSKSQLDDLYRALTAHPMVKVVL"
     gene            complement(6992..7840)
                     /locus_tag="PKW44_01160"
     CDS             complement(6992..7840)
                     /locus_tag="PKW44_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid aminotransferase"
                     /protein_id="HQR50228.1"
                     /translation="MVYLNGSFLPIEQATVPVLDRGFIFGDGVYEVIPVYSRHPFRLD
                     EHLRRLQNSLDGIRLANPHSNEEWSRLIRELIRMDANEDQSVYLHVTRGVARRDHPFP
                     VGVAPTVFMMSNPLITPDATLVEHGVAAISAVDNRWGRCNIKAISLLPNVLLRQLAVD
                     AGANETVLFRDGILTEGTASNIFAVEQGVILAPPKDHYMLPGITYDLLLELAAAHGIP
                     VQIGKFEESRIRNADELWLSSSTREVLAITTLDGKPIGNGQPGPLFRKMVALYQDYKN
                     NVMRNG"
     gene            complement(7854..9002)
                     /locus_tag="PKW44_01165"
     CDS             complement(7854..9002)
                     /locus_tag="PKW44_01165"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HQR50229.1"
                     /translation="MRLLALLFTLLFSLIASAGDVPPPPTVSGKSYILSDFNSGAVLA
                     EQNSHERISPASLTKLMTAYLSFKAIKQGHLKLDQTVPVSENAWKGRIGCGGAQSSCM
                     FIAPDIPVTVDELLHGMIIQSGNDACVALAEAIAGSEQAFADLMNKEAARLGMKDTQF
                     RNASGLDEDGHYTTAYDLSLLAAALIRDFPDQYDRLYKVKEYTYNNIKQMNRNRLLWI
                     DPAVDGMKTGHTSSAGYCLIASAKHGNMRLISVFAGAPTEKARIIDSQKLLNYGFLFY
                     ETRPLYRKGQSISKLRVWKGQEQTLDATVAEDLYVTLPKGNHDKLSAQVTSKQPLIAP
                     VSAGQDVGSLQVALDGKPLLQRKLVAAKEVPIAGIFGRMWDSLKLMFN"
     gene            complement(9038..9949)
                     /locus_tag="PKW44_01170"
     CDS             complement(9038..9949)
                     /locus_tag="PKW44_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HQR50230.1"
                     /translation="MKKYGLGTLWPAALLLAFFLGGCGTTGQVKSEKPKTAADSQSRD
                     GASGGKKPGGYYLDDGPGDNPPPDIDAIPDAVPRPEQPLARANRPYMALGENYTPMTS
                     YQPYKASGVASWYGRRYHGQKTSSGEIYDMYSMSAAHPVLPIPSYVRVTNPENGKSVV
                     VRVNDRGPFKHDRLIDLSYAAAYKLRLTGKGSGQVEVEAISPLADNGGATTRPAESQD
                     KSAGITSSPAGNYVQAGAFKLKENADQLRQKLQQQDLAGNVAVENWYNDGVYRVRLGP
                     YASRDEAERAAGQIKRALSISTLVVTQ"
     gene            complement(9960..11054)
                     /gene="rodA"
                     /locus_tag="PKW44_01175"
     CDS             complement(9960..11054)
                     /gene="rodA"
                     /locus_tag="PKW44_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HQR50231.1"
                     /translation="MINLSLKRLTAHFDQFLLGALMLTLVAALFILYSASGQSMERMT
                     AQAVNILVALAVLWMFANIPPHHLQRAALPLYVLGILLLVGVLLFGDVSKGARRWLNL
                     GVMRIQPSELMKIATPMMLAWYFAKREATLRFSDHVVGAVMLTLPVGLIVKQPDLGTS
                     LLIGASGFYVLFLAGLSWRLLIGLGVVAGAAIPLVWPLLHDYQRRRVEILFDPSQDPL
                     GAGYHTIQATIALGSGGLAGKGWLNGTQSQLDFLPERHTDFIFAVFGEEFGLVGNLLL
                     LLLFSVIIARGLVIAANAQTMFTRLLAGSITLTFFTYAFVNMGMVSGILPVVGVPLPL
                     ISYGGTSLVTLMIGFGILMSIQTHKKLVTS"
     gene            complement(11047..12975)
                     /gene="mrdA"
                     /locus_tag="PKW44_01180"
     CDS             complement(11047..12975)
                     /gene="mrdA"
                     /locus_tag="PKW44_01180"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HQR50232.1"
                     /translation="MPSASELRNPLREQHYFKLRLIAAALVVLICFGILATRFSYLQL
                     VRYEHYQTLAENNRISLVPMVPNRGLILDRNGVVLAHNFFVYTLEITPSRVENLEETI
                     NALKELVEISPSDHKRFKRLRDESHNFESVLLRSHLSDEEAARFAVNRYRFPGVDIRS
                     RLFRHYPLGTSSSHLIGYIGRINDADLKGLEKNGDLSNYKGTDHIGKAGLEQYYERHL
                     HGVTGFQQVEVDADGHAVRVLSSAAPVPGDNLVLSIDSKLQAIAEQAFGSRRGAMVAL
                     DPASGEVLAYVSRPGFDPNLFVDGIDPESWKALNESLDKPLINRPLRGAYPPGSTFKP
                     FVAMAGLEAGEREPPFSISDPGFYMLPGSSHQYRDWKPGGHGSVDIQRAITVSCDTFF
                     YGLARDLGIERLAGFVSHFGFGRKSGIDMDGELSGLLPTPQWKAKRWKQPWYPGETLI
                     TGIGQGYTLVTPMQLAQATAILANNGVAMRPRLVSSIQQGIGGTDEPTALQTMDRVQL
                     KPEHVELVKQGMTDVTRPGGTAASVGAGAPYNIAGKTGTAQVVGIKQGQKYNEHAIDE
                     RHRDHALFIAYAPAEAPRIALAVIVENGGHGGSAAGPIARQVMDYYLLGKEPSPPALP
                     QGEAPEATNAQTEPEPHD"
     gene            complement(12985..13458)
                     /gene="mreD"
                     /locus_tag="PKW44_01185"
     CDS             complement(12985..13458)
                     /gene="mreD"
                     /locus_tag="PKW44_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HQR50233.1"
                     /translation="MHPSHKHVYLSLFAAFILYLLPWQGFGLMLRPDFVLLVLIYWML
                     RAPHLCNVGTAWTIGLLVDLASGGLFGQYALAYAISAFVALTYQRRLVLFNAWQQAAY
                     VLALLLLTQTTILVLKLFSGGGLPGWSYFLPSVSGVLLWQLLFSRIRAANVKDSG"
     gene            complement(13442..14347)
                     /gene="mreC"
                     /locus_tag="PKW44_01190"
     CDS             complement(13442..14347)
                     /gene="mreC"
                     /locus_tag="PKW44_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HQR50234.1"
                     /translation="MDPYQPHAFFARGPSTLVRLIFFGTLSIVLMAVDSRLHTLTEVR
                     QGIVALLHPLEVIVHSPVAAYHQVSEYLSTQNTLTRENRHLREQALRQGAELQRFHTL
                     LEENEHLRNLLGVVPALAQPARLAEIVHAGRDPFTHKVIVNLGSQQGISSGQAAVDEY
                     GVIGQVTRVYPFSSEVTLVTDKDLTIPVQIERNGLRAIAFGNGRERAINLPYLPANVD
                     VREGDTLVTSGIDGIYPSGMAVAKVTRIERAADSPFAHIVCTPTAGTETHRQILLLAV
                     AEKPPQLPPPLEQPNPSPARHASKP"
     gene            complement(14393..15436)
                     /locus_tag="PKW44_01195"
     CDS             complement(14393..15436)
                     /locus_tag="PKW44_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HQR50235.1"
                     /translation="MFGFLNSYFSNDLAIDLGTANTLIYVRGKGIVLDEPSVVAVRHE
                     GGPTNKKTILAVGAEAKRMLGRAPTNIQAIRPMKDGVIADFTITEQMLKYFIRRVHES
                     RLFSPSPRIIICVPVGSTQVERRAIKESAEMAGARQVFLIEEPMAAAIGADMPVAEAT
                     GSLVVDIGGGTTEVGVISLGGIVYAKSERVGGDKFDQSIIDYIRRNYGMQISEPTAEL
                     IKKKIGSAFPGSEVLEMEVTGRNLAEGLPRKFTISSNEILEALTEPLNAIVGAVKSAL
                     EQTPPELGADIAEKGMVLTGGGALLRDLDRLLMEETGLPVIVADDPLTCVARGCGRAL
                     EEMDRLGNVFANE"
     gene            15552..15839
                     /gene="gatC"
                     /locus_tag="PKW44_01200"
     CDS             15552..15839
                     /gene="gatC"
                     /locus_tag="PKW44_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HQR50236.1"
                     /translation="MSLTTDDVKRIAHLARIEVTEDDAQATLSKLSGILGLIEQMQAV
                     DTTGIAPMSHSQDVSQRLRDDVVIETNQRELFQSIAPAVENGLYLVPKVIE"
     gene            15839..17290
                     /gene="gatA"
                     /locus_tag="PKW44_01205"
     CDS             15839..17290
                     /gene="gatA"
                     /locus_tag="PKW44_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HQR50237.1"
                     /translation="MINASLKELAAALASKQISSAELTQLFLDRIRRLNPEINAYVTL
                     DAERSLAQASAADALIAQGKFGPLTGIPFAQKDIFCAKGWRTTCGSKMLENFIAPYDA
                     HIISQFDAAGAVNLGKTNMDEFAMGSSNETSYFGKVQNPWDRARVPGGSSGGSAAAVA
                     ARLAPAATGTDTGGSIRQPASLCGLSGLKPTYGICSRYGMIAFASSLDQAGPMAKSAE
                     DLALMLNVMAGFDSRDSTSLQREKEDYTRDLNKPLAGLRIGLPREYFAEGLDAGVAKV
                     VLDAIDQYRKLGAETVEISLPNTQLSIPVYYVLAPAEASSNLSRYDGVRYGHRAAEYT
                     DLLDMYSKSRAEGFGAEVKRRILIGTYVLSASYYDAYYLKAQQLRRLIAQDFEQAFKQ
                     CDVIMGPAAPGTAFKTGEKTDDPVAMYLEDLYTIAVNLAGLPGMSVPAGFTNGLPVGL
                     QIIGNYFDEARMLSVGHQYQQVTDWHTRMPEGI"
     gene            17291..>18127
                     /gene="gatB"
                     /locus_tag="PKW44_01210"
     CDS             17291..>18127
                     /gene="gatB"
                     /locus_tag="PKW44_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HQR50238.1"
                     /translation="MQWEVVIGLETHTQLQTKTKIFSGASTAFGAEPNTQADAVSIAL
                     PGVLPVLNREAVRCAVKFGLAIDAEIGRRSVFARKNYFYPDLPKGYQISQYELPVVGR
                     GHLTIQVGDEEKTVRITRAHLEEDAGKSLHEDFHGMTGIDLNRAGTPLLEIVTEPDMR
                     SAAEAVAYAKKLHELVMWIGICDGNMQEGSFRCDANVSVRPKGSDKLGTRREIKNLNS
                     FRFMQQAIEYETRWQIETLEDGGTIEQATVLFNPDTGETRAMRSKEEANDYRYFPDPD
                     LLP"
BASE COUNT         3796 a         5512 c         5323 g         3496 t
ORIGIN      
        1 cacgagcgca ccttaatggt gcatttaatg atgtagcgcc ttattttggt gcatctcatt
       61 ttcgttttct tttctcagcg cctgtgtgaa aatgtcaaat tacttacttt ttcatggcac
      121 gattcctgct tttttctcgt gtctttattt tgtttaaccg ttctaggaga ttgaaatggc
      181 agtcgccgac gtactcaaga tggtcaagga caatgaaatc cgtttcgttg acttccgctt
      241 taccgacacc cgcggcaagg agcagcacgt ttctgtgcca gtttccgctt tcagcgaaga
      301 caagttcacc gaaggccacg cttttgatgg ttcctccatt gccggctgga agggcattca
      361 ggcttccgac atgctgctga tgccggatcc ggacaccgct ttcgtcgacc ccttcttcga
      421 cgagccgacg ctggttctgt cctgcgacgt agtcgatccg accgatggcc gtggctacga
      481 ccgcgacccg cgttcgatcg caaaacgtgc cgaagcttat ctcaagtctt ctggcttggg
      541 cgacaccgcc tatttcggcc cggagccaga attcttcatt ttcgactccg ttcagtggga
      601 agtcggcatg tcgggttgtc acgtcaagat cacttccgaa gaagcggcat ggacttccgg
      661 cgaaagcctg gaaggtggca acatcggcca tcgccccggt atcaaaggag gttacttccc
      721 ggttcctccg gtcgacagcc tgcatgacat ccgctccgcc atggttatgg cgctggaaga
      781 aatcggcatc ccggtggaag tacaccacca cgaagtggcg actgccggcc agtgcgaaat
      841 cggtaccgtg ttcaatacgc tggtaaagcg tgccgactgg actcagcagc tgaagtacat
      901 cgtacataac gtggcccacc agtatggcaa gaccgcgacc ttcatgccca agccgatcgt
      961 tggcgacaac ggttccggca tgcacgtaca ccagtccgtg tggaaggatg gcaagaacct
     1021 gtttgcaggc aatggttacg ctggcctgtc cgaatttgcg ctgtactaca tcggcggcat
     1081 tatcaagcac gtcaaggcgc tgaatgcgat caccaacccg ggcaccaact cttacaagcg
     1141 tctggttcca cactacgaag caccggtgaa ggtggcctat tccgccaaga accgctccgc
     1201 gtccatccgc gttccgcacg tgcagtccga caaggcgcgc cgcatcgaga cccgtttccc
     1261 ggatccgatc gccaatccgt acctcgcgtt ctccgcgctg ctgatggcag gtctggacgg
     1321 cgtacagaac aagattcacc ccggcgatcc ggctgacaaa aatctgtacg acctgccgcc
     1381 ggaagaagac gccaagatcc cgaccgtgtg cgccagcctc gacgaggcac tggacgcgct
     1441 ggacaaggat cgcgagttcc tgacccgcgg cggagtattc tccaacgaat ggatcgatgc
     1501 ttacatcggc ctgaagatgg aagaagtgaa caagatccgc atgaccacgc atccgatcga
     1561 gttccagatg tattacagct gctaatcggc tgctattgaa taaaaaaggc ggggaaaccc
     1621 gcctttttta ttgcccgctc cgtcaacatc atatccggca accgcgttgc tcaggcaggc
     1681 gttctgctct acacttagcc gcatgaaaaa gctattgttt atcctgatcg ggcttggcat
     1741 tcagctgggt gctcatgccg aaatatacaa gcaagtcgat gccgatggac acgtgactta
     1801 ttccaacgtg cccatcaagg gcgcgaccaa gctcaacctc gatccgccac cctcaggcag
     1861 cagttccacc aaggcgcccg cacgtactgc cacccccgcc aattttccgc gcgtggatca
     1921 ggaaacccag cgccagcgcg acgacaagcg ccggcagata ctggaagatg aactcgccaa
     1981 cgaaaagaaa gcactggaag aggccagaaa gaactacgtc gagggcgaag ccaatcccga
     2041 aatgttcaca gcagcaggcg gcaagagatt ccgcaatgtt cccaagtatg aggaaaagat
     2101 gaagggcttg caggaggacg tatcgcagca cgaaaaaaac gtcgagttgc tggaaaaaga
     2161 attaggctcc ctcaagtaaa tccagccgca gcctcttctg gcctgactct tgctttccag
     2221 gaaaggtgat gacacacagc gtacccagca ctttctccgg ccttgagcac ctttccaccg
     2281 cagttctgtt gctggatgac cagggcttcg tcctttacac caaccccgca gcggagctgc
     2341 tgttcgccgt cagcgccaac cagatcaatg gccataccct ggcgcaagtg ttcccgggct
     2401 gcgatatcct gctcgccgca gtgacgagct cgctcgccag caatagtccg ttccgcgaac
     2461 acgaattcct catcaccacg ctgcgccatc attcgtttgc cgcaacctgc accgcaacgc
     2521 cggtagaatt ggccccggcg cggctggtgc tggagttcca gcaaatggac cagcagctgc
     2581 gcatcgcgcg cgaggaacgc atgctgatcc agcagcaggc caatgccgag ctgctgcgca
     2641 atctggcgca tgaaatccgt aaccccctgg gcgggctgcg tggcgctgcg cagctgctgg
     2701 agcacgaact gccgcagccc ggcctgcgtg aatacaccca ggtcattatc aaggaggcgg
     2761 accgcctgca acagctgatg gatcgcctgc tggtaccaca ccgcgtaccg aaatacgaac
     2821 cgaccaacat ccacgaagtg ctggagcgcg tgcgcagcct gctgctggca gaatctccgc
     2881 aaggtgtatc gatctggcgc gactacgaca ccagcctgcc ggacctgatc ggcgaccgcg
     2941 aaaaactgat ccaggcggta ctcaacatcg cccgcaacgc ggtacaggcc atgcaggggc
     3001 agggcgaaat cgtgttccgc acccgcgccc agcgccaggt gtcgctggca aaaaagcgct
     3061 accgcgtcgc catcaagcta cagatcatcg acaacggccc gggcatcccg aaagaaattc
     3121 gcgaccgcat tttttatccg ctggtgtccg gccgcgcaga aggctccggc ctcgggctaa
     3181 cgctggcgca aaccttcatc acccagcacc acggcatgat cgactgcgaa agtgagccag
     3241 gccgaacctg cttcaccatc ttgttgccgc tggaagcaac cgcttacaaa ccaacacatt
     3301 tatcagaaca ggcaaaagct ccatgaaacc aatctggatc attgacgacg atcgctctat
     3361 ccgctgggta ttcgaaaagg ccttggcacg caccgacatg gaattcagaa ctttcgcctc
     3421 tgtgccggaa gccatggatg cactggccga tgacgaaccg caggtggtcg tcagcgacat
     3481 ccgcatgccc aacggttccg gactggattt tctgcaggag atcaagcgcc gcctgccaga
     3541 tatcccggtc atcatcatga ccgcctattc cgacctggaa agcgcggtgg ccgcgttcca
     3601 gggcggggcg ttcgaatacc tggccaagcc gttcgacgtg gaccaggcag tggacatcat
     3661 ccgccgcgca gtggacgaaa gcatgcgcca ggccacggaa agcgaggagc cgcaggaaac
     3721 gccagaaatc atcgggcagg cgccggcgat gcaggaagta ttccgcgcca tcggccgctt
     3781 gagccgctcg catgccaccg tgctgatcaa cggcgagtcc ggcaccggca aggaactggt
     3841 ggcgctggcg ctgcaccgcc acagtccgcg tagcgaaaaa ccattcattg ccatcaatac
     3901 cgcggccatt cccaaggact tgctggaatc cgaactgttt ggccacgaac gcggcgcctt
     3961 caccggcgcg caggccgcgc ggcgcggccg cttcgagcag gctgatggcg gcacgctgtt
     4021 tctggatgaa atcggcgata tgccaccgga cctgcaaacc cgcttgctgc gggtgctgtc
     4081 cgatggccag ttctatcgcg ttggcggtca ccagccggtc aaggtcaacg tgcgcatcat
     4141 cgctgccact caccaggatc tggaggaccg cgtcaggcag ggcctgttcc gcgaagacct
     4201 gttccaccgc ctgaatgtct tcccgcttcg cctcccgaca ttgcgggaac gccgcgaaga
     4261 tatcccgttg ctggcacgcc actttctgca gcagagcgca ctggaactcg gggtggaacc
     4321 caagcagctt gcacctgccg ccttacgcta cctgtcaggc ctgggctgga gcggcaacgt
     4381 gcgacaactg gaaaacgtct gtcactggct taccgtaatg gcgccggggc aaaatatcga
     4441 tattgccgac ctgccgccgg aagcgcgcga agaacatggc cgccctgcaa acacggcttc
     4501 ctggcaggaa gcactggcgc aggaagtggc ggatgctctc agccgcggag agcagaatat
     4561 tctggaagca cgcgcccagg aattcgagcg catcatgatc agaaaggcgc tggagcacac
     4621 cggcggccgc cgcattgaag ccgccaccca actcggcatg gggcgcaaca cgctgacgcg
     4681 caaaattcag gaattggaaa tcgaggactg acgctagcgc aggctacaca cccgcctcat
     4741 gcgcctgcat atctgcatga tagcttgagc gcaccatcgg cccgcaggcg gcattggtga
     4801 aacccattgc cagcgcctgc cgttcaaacc actggaaacg ctgcggctcg acgaagcgca
     4861 gcaccggaag gtggtgcgcg ctgggctgca gatactggcc gagggtgagc atttccacgc
     4921 cgtggccgcg caggtcgcgc atcacctcga aaatttcctc gtcggtttcg cccagtccca
     4981 gcatgaggcc agacttggta ggaatccccg gaaagcgcgc cttgaagttg gccagtagtt
     5041 tcagcgagtg cgcatagtcc gcgccgggcc gggcctgctt gtagaggcgt ggtacggttt
     5101 ccaggttgtg gttcatcacg tccggcggcg cctcagcgag cagttcgagc gcgacatcca
     5161 gccgaccacg gaaatccggc accagcacct cgattctgag atgtggcgaa tacgcgcggg
     5221 caatgcgaat gcaatcggca aaatgccgcg ccccgccgtc gcgtaggtca tcgcggtcga
     5281 cgctggtgat caccacgtat ttcagcctca tggcggcgat ggtgcgcgcc agattttccg
     5341 gctcctcggc atccggcggc aacggtttgc cgtgcgccac gtcacagaac ggacagcgcc
     5401 gggtgcaaag atcgccgagg atcatgaagg tggcggtgcc gccactgaag cactcgccga
     5461 tgttggggca ggatgcctcc tcacataccg tgtgcaggct gttgtcgcgc aggatctgct
     5521 tgatttcctg gaagcgcggg ccgtccggca ccttcatgcg gatccattcc ggcttgcgca
     5581 gcgctggctg cggcacgatc ttgatcggga tgcgcgcagt cttggcggcg cctttctgct
     5641 tggtctgggc gttatccatg atggttattg gtacgttttg ccgttatgcc tgacccatcc
     5701 tgccgcatgc tggccggccg gatcatggtg tgtccaatgg atgaccccac cttttgcatt
     5761 ccactcgtat tccccataaa acgaaaccct atctccttcg ctcaagccat ccaccctggg
     5821 tgcgagatca atgttatgcg caagcaacaa agtttgacca ttggcaagcc ggacgataaa
     5881 ccgttgatgc ctgctgccat tgacgtcgtc gcccagtatt ctcgataccg tacccccgcc
     5941 ttcaacctgg acattgctct ggcgttgctc gaatgcgact gcaattggct ggtcgctggc
     6001 ggcagcaacc tcgctctgat tgctgaacaa gcttgcagga ctggaagatg tctcatgcgt
     6061 ttgcgcaaac tggaagtagg ctacaaccag aatggcgatg agcagaagct tcttcatttc
     6121 agcttattca actttcgaga gatttcatca tggcagcggt cagcctgctc gcatactcac
     6181 ctgatccatg aatgataccc aaatcccgca tttgcgtgac ctgcagtccc cggtaaccgc
     6241 atggatcgat ggcctcgaac ggggacaaat ccatatccac attcaggctg agaccgtggt
     6301 agcaacagcc gttcttcagg cgcagaccaa gcgaggcgat tttttttccc tccacataaa
     6361 caccaggcgc atccggccgg ccttgcgagg taatgccgta ttctgccagc aagttgatga
     6421 tggcgccctc catgcagtcg accagttggc gtatcttgag gccgctacgc ttcagatcga
     6481 gcagcagata gagcacgatc tggccggggc cgtgataggt gatcttaccg ccgcggtcgg
     6541 tgagcaccag cgggatgtcg tcgcgccaag gcaggcgcac gtccttgcgg ttgagtccta
     6601 aggtatagac cggcgggtgc tcagtcagcc acaattcgtc tggggtatcg gcctgacgct
     6661 gggcggtaaa atcctgcatg gcgcgccagg tggcctcgta gtcagtcagg ccgagatgtc
     6721 ttgccagcat tgctagagca cgaccttgac catcgggtgc gcggtcaggg cgcgatagag
     6781 gtcgtccagc tgtgatttcg aggtggcgtt gatggtacaa gtcaggctca tgtatttgcc
     6841 gccgctactc gcgcgcattt ccacgcacgt ggcatcgaaa gccgggtcgt gcccgcgcac
     6901 taccgcgacc accgcttcga caaagccgtc gcgtgtttcg cccatcacct tgagtggaaa
     6961 ctcgcaaggg aattccagca gggtttcctg atcagccatt tcgcatcacg ttgtttttgt
     7021 aatcctggta aagcgcaacc atcttgcgga acagcgggcc gggctggcca ttgccgatgg
     7081 gtttgccgtc cagcgtcgtg atggccagca cctcgcgggt ggaggacgac aaccacagct
     7141 catctgcgtt acggatgcga gattcttcga attttccgat ctgtaccgga atgccatgcg
     7201 ccgcggcaag ctcgagcaac aggtcgtagg tgatgcctgg cagcatgtag tgatccttgg
     7261 ggggggcgag aatcacgccc tgctccacgg caaagatgtt gctggcggtg ccttcggtca
     7321 ggatgccgtc gcggaacagg acagtttcgt tggcgccggc atcgacggcc aactggcgca
     7381 gcagcacgtt aggcagcagg gagatggcct tgatattgca acggccccag cgattatcga
     7441 ccgccgagat cgccgcgacg ccatgctcga ccagcgtggc atccggcgta atcagcggat
     7501 tgctcatcat gaacacggtc ggtgccacgc cgaccggaaa cgggtgatct cgtctggcga
     7561 caccgcgggt gacatgcaga tagaccgact ggtcctcgtt ggcatccatc cggatcaact
     7621 cccggatcag gcgcgaccac tcctcgttgg aatgagggtt ggcgaggcga atgccgtcga
     7681 ggctgttctg caatcgccgc aggtgctcgt ccagccggaa cggatggcgc gaatacaccg
     7741 ggatgacctc gtacacgcca tcgccgaaga tgaagccgcg atcaagtacc ggcacggtag
     7801 cctgctcgat gggcaggaag cttccgttga ggtagacgat ctgcgccacg ggcctagttg
     7861 aacatcaact tcaggctatc ccacatacga ccaaaaatac cggcgatcgg gacctccttc
     7921 gccgccacca acttgcgttg cagcaggggc ttgccatcaa gagcgacttg cagggaacca
     7981 acatcctgcc cggcgctgac cggagcgatc agcggttgct tgctggtaac ctgcgcactc
     8041 agcttgtcgt ggttgccttt gggcagggtg acataaagat cttccgccac cgtggcgtcc
     8101 agtgtttgtt cctggccttt ccacaccctg agcttggaga tcgactgccc ttttctgtac
     8161 aacggacggg tctcgtagaa caggaaaccg taattgagca gcttctggct gtcgatgatg
     8221 cgggcctttt ccgtcggcgc gccggcgaat accgagatca ggcgcatgtt gccgtgcttg
     8281 gcggaagcga tcaggcaata accggcactg ctggtgtggc cggttttcat gccatccacc
     8341 gccggatcaa tccacagcag gcggttgcgg ttcatctgct tgatgttgtt gtaggtgtat
     8401 tccttgacct tgtacaaacg gtcgtactgg tcggggaaat cacggatcag cgccgcggct
     8461 aacagcgaca aatcgtaggc ggtagtgtag tggccgtcct cgtcgaggcc gctggcgttg
     8521 cggaactggg tgtccttcat gcccaggcgc gcggcctcct tgttcatcag gtcggcaaac
     8581 gcctgctccg agccggcaat agcttcggcc aaggcaacgc aggcatcgtt gccggactgg
     8641 atgatcatgc cgtgcagcag ttcgtccacc gttaccggaa tatccggcgc gatgaacatg
     8701 caggagcttt gcgcaccgcc acagccgata cggcctttcc aggcgttctc gctgaccggc
     8761 acagtctggt cgagtttcag gtggccttgc ttgatcgcct tgaacgacag gtaggccgtc
     8821 atcagcttgg tgagcgaggc cggtgaaatg cgttcgtgag aattctgctc ggccagcacc
     8881 gcgccactat tgaagtcgct caggatgtag gactttccgg aaaccgtggg cggcggcggc
     8941 acatcgccgg cactggcgat aaggctaaaa agcaaggtaa aaagcagggc aagtaggcgc
     9001 atagggaact ctgtagttag tgcaacttag gaattaatca ttgggtgaca accagggtgg
     9061 aaatgctaag cgcccgcttg atctgaccgg cggcacgctc ggcttcgtcg cggctggcat
     9121 aggggccaag ccgcacgcga tagacgccgt cattatacca gttctccacg gcaacatttc
     9181 cggcaagatc ctgctgttgc agcttctgac gcaactggtc ggcattttcc ttcagcttga
     9241 aagcgccggc ctgtacgtag ttgccggctg gtgacgatgt gatgccggcg gatttgtcct
     9301 gactttctgc cggccgcgtc gtcgcccccc cgttgtcggc caagggggat atggcctcga
     9361 cctccacctg tccgctaccc ttgcctgtca ggcgcagctt gtaggcggca gcgtaggaca
     9421 ggtcgattag ccggtcatgc ttgaacgggc cgcggtcgtt aacgcgcact accaccgatt
     9481 tgccgttctc gggattggtg acgcgcacat agctcgggat cggtagcacc ggatgtgcag
     9541 cagacatact atacatgtcg tatatttcac cactggaagt cttctggccg tggtagcgcc
     9601 ggccatacca ggacgcgaca ccacttgcct tgtaaggctg gtaactggtc atcggcgtgt
     9661 agttttcacc cagcgccata taagggcggt tggcgcgtgc cagcggctgt tccggccgcg
     9721 gtacagcatc ggggatggcg tcgatatcag gcgggggatt gtcgcccggc ccatcgtcca
     9781 ggtaataacc acccggcttc tttccgccgg atgcgccgtc ccttgactgc gaatccgccg
     9841 cggtttttgg cttttcgctt tttacctgac cggtcgtgcc acaaccaccc aaaaagaacg
     9901 ccagtagcag cgctgccggc cacaacgtcc cgagcccgta ttttttcata cccgcctcct
     9961 cacgacgtca ccagcttctt gtgcgtctga atactcatca agataccaaa gccgatcatc
    10021 agcgtcacca gtgaagtgcc accgtaactg attagcggca acggcacccc caccaccggc
    10081 agaataccgc tgaccatgcc catgttgacg aaagcatagg taaagaaggt cagcgtaatg
    10141 ctgcctgcaa gcaaacgggt gaacatggtc tgggcattgg ctgcgatcac caggccgcgt
    10201 gcaatgatga cgctgaacag cagcagcagc aagagattgc cgaccaggcc gaactcctcg
    10261 ccgaacacgg cgaaaataaa gtcggtatgc cgctccggca ggaaatcaag ctgcgactgc
    10321 gtgccgttga gccaaccctt gccggccaat ccgcccgacc ccagcgcgat ggtggcctgg
    10381 atagtgtgat agccagcacc gagcggatcc tgcgaagggt caaacaggat ttccacccgc
    10441 cgtcgctgat aatcgtgaag cagtggccag accagaggaa tcgcggcgcc tgccaccaca
    10501 ccaaggccaa tcagcaggcg ccaggagaga cctgccagga acagcacata aaagcccgaa
    10561 gcaccaatga gcagagatgt gcccaggtcg ggctgcttga cgatcaagcc caccggcaac
    10621 gtcagcatca ccgcaccgac gacatggtcg gaaaagcgca acgtggcttc gcgcttggcg
    10681 aagtaccagg ccagcatcat cggtgtcgcg atcttcatca gctccgaagg ctggatgcgc
    10741 atgacgccca gattgagcca gcggcgcgcg cccttggaaa cgtccccgaa cagcagcacc
    10801 ccgaccagca gcaggatgcc cagcacgtaa agcggcaagg ccgcacgctg caggtggtga
    10861 ggcgggatat tggcaaacat ccacagcacg gccagcgcca ccagaatatt gactgcctgc
    10921 gcggtcatcc gctccatgct ctggccggag gcgctataaa gaatgaacag tgccgcaacc
    10981 agggtcagca tcagagcccc gagcaggaac tggtcgaaat gtgcggtcag gcgtttcaga
    11041 gacaggttaa tcatgaggtt ccggctccgt ctgtgcgtta gtggcttccg gcgcttcgcc
    11101 ctggggcaga gcgggtgggg acggttcctt gcccagcagg tagtagtcca ttacctggcg
    11161 cgcaatcggg ccggcggccg agccgccgtg tccgccattc tcgacgatga ccgccagcgc
    11221 gatcctcggc gcttccgccg gggcgtaggc gatgaacagg gcatggtcgc gatggcgttc
    11281 gtcgatggcg tgctcgttgt atttctgtcc ctgcttgatg ccgaccacct gcgcggtgcc
    11341 ggtcttgccg gcaatgttat agggcgcgcc ggcacccacg ctggcagcgg tacccccggg
    11401 gcgggtgaca tccgtcatcc cttgcttcac cagttccaca tgctcgggct tgagctgaac
    11461 acgatccatg gtctgcaatg cggtgggttc gtcagttcca cctatacctt gctgaatgct
    11521 gctgaccagg cgcgggcgca tcgcgacgcc attgttggcc aggattgcgg tcgcctgcgc
    11581 cagttgcatc ggcgtgacca gcgtatagcc ctggccgatg ccggtaatca gggtttcacc
    11641 cggataccat ggctgtttcc agcgcttggc cttccattgt ggcgtcggca gcaatcccga
    11701 gagctcgccg tccatgtcga tgccggactt cctgccaaag ccaaaatggc tgacgaaccc
    11761 ggccagccgc tctatgccga ggtcgcgcgc aaggccatag aaaaacgtgt cgcaagaaac
    11821 cgtgatggcg cgctggatat ccacgctgcc atggccgccc ggcttccagt cgcgatattg
    11881 gtgagaactg cccggcagca tgtagaaacc aggatcactg atgctgaacg gcggttcgcg
    11941 ctcgccggcc tcgagccccg ccatggcgac gaaaggcttg aaggtagacc cgggggggta
    12001 ggcaccgcgc aaggggcggt tgatcagcgg tttgtccagc gattcgttga gcgctttcca
    12061 gctttccggg tcgatgccat cgacaaacaa gttggggtcg aaccccggcc ggctgacgta
    12121 agcaagcacc tccccgctgg cagggtccag cgccaccatc gccccgcggc ggctaccgaa
    12181 ggcctgctcg gcgatggcct gcagcttgct gtcgatcgac agcaccagat tgtcgccggg
    12241 caccggcgcg gcgctggaaa gcacgcgcac ggcatgaccg tccgcgtcga cctcgacctg
    12301 ctggaatccg gtcacgccat gcagatgacg ctcatagtac tgctccagcc ccgccttgcc
    12361 gatatggtcg gtgcccttgt aattggacag gtcgccattt ttctcgagcc ccttcaggtc
    12421 agcatcgttg atgcggccga tataacctat caggtgagaa ctggaagtgc ccaacggata
    12481 gtgccggaac aggcgcgaac ggatatctac cccgggaaaa cggtaacggt tgacggcaaa
    12541 gcgtgccgcc tcctcatcgc tgaggtggct gcgcaatagc acgctttcga aattatggct
    12601 ttcgtcgcgc aaccgtttga aacgtttgtg gtcggacggg ctgatttcca ccagctcctt
    12661 cagtgcattg atagtttctt ctaggttttc cactctggaa ggggtgattt ccagggtata
    12721 gacgaagaag ttgtgcgcca gcaccacgcc gttgcggtcc agaatcaaac cgcgattggg
    12781 caccatcggt accagcgaga tgcggttgtt ctctgccagc gtctggtagt gctcgtagcg
    12841 caccaattgc agataagaaa aacgcgtggc gagaatgccg aagcagatca gcactaccag
    12901 cgccgccgcg atgaggcgaa gtttgaagta gtgctgctcg cgcagggggt ttctgagctc
    12961 ggaagcggat ggcatgtgat gccgttagcc ggaatctttg acattggccg cacggatgcg
    13021 cgagaacagc agctgccata acagcactcc gctgacactg ggcaggaagt agctccagcc
    13081 aggcaaacct ccgccggaaa acagtttgag caccaggatc gtagtctggg tgagcagcag
    13141 cagcgccagc acgtaagcag cctgctgcca tgcattgaat agcaccagcc ggcgctggta
    13201 ggtgagggcg acaaaagcgc tgatggcata agccagcgca tactggccaa acagcccgcc
    13261 gctggcgaga tcgaccagca gtcctatggt ccaggcggtg ccgacgttgc acagatgcgg
    13321 cgcgcgcagc atccagtaaa tcagcacgag cagcacgaag tcggggcgca gcatcagtcc
    13381 gaacccctgc cagggcagca ggtagagtat gaatgccgcg aacaacgaca ggtatacgtg
    13441 tttatggctt ggatgcatgg cgggcagggg atgggttcgg ctgctcaagg ggtggaggca
    13501 actgcggcgg cttttccgcc acggcaagca gcaggatttg ccgatgggtt tcggtgccag
    13561 cggtcggcgt acagacaata tgggcaaatg gggagtcggc ggcgcgctcg atgcgggtga
    13621 ccttggctac cgccatgccg ctcgggtaaa tgccgtcaat gccggaggtt accagtgtgt
    13681 caccctcgcg gacatccacg ttggccggca ggtatggcag gttgatggca cgttcgcgtc
    13741 cattgccgaa ggcgatggcg cgcaggccgt tgcgctcaat ctgaaccggg atggtcaaat
    13801 ccttgtcggt caccagcgtc acctcgctgc tgaaaggata aaccctggtc acctggccga
    13861 tgacgccgta ttcgtccacc gccgcctgcc cggaactgat gccttgttgc gagccgagat
    13921 tgacaatcac cttgtgcgta aagggatcgc ggccggcatg tacgatttcc gccagcctgg
    13981 ctggctgcgc cagggcaggt actacgccga gaagattgcg cagatgttcg ttttcctcaa
    14041 gcagcgtatg aaaacgctgc aattcggcac cctgtcgcag tgcctgttcg cgcagatgac
    14101 ggttttcccg ggtgagcgtg ttctgcgtgg aaaggtattc actcacctga tggtaagcgg
    14161 caacagggct gtggacgatc acttccagcg gatgcagcaa tgcaacaatc ccctgccgga
    14221 cctccgtcag ggtgtgcagc cgtgaatcaa ccgccatcag gacgatggaa agcgtgccga
    14281 aaaatatcag acgcaccaag gtgctggggc cgcgggcaaa aaaggcgtgc ggctgataag
    14341 ggtccatacg ggctctcagc gaaaaaccat ctgctaacgg tcagccgccg ctttattcat
    14401 tggcaaagac gttgcccagc ctgtccattt cttccagcgc acgtccacag ccgcgagcga
    14461 cacatgtcaa cggatcgtcg gccacgatca ccggcaaacc ggtttcttcc atcagcaggc
    14521 gatccaggtc gcgcagcagg gcaccgccgc cggtcagcac catccccttt tcggcgatgt
    14581 cggcgcccaa ttccggcgga gtttgttcca gcgccgactt gaccgccccg acgatggcat
    14641 tgagtggctc ggtcagcgct tccaggattt cgttgctgga aatggtgaac ttgcgcggca
    14701 gaccttccgc cagattgcgg ccggtgactt ccatctccaa cacttccgag ccggggaaag
    14761 cagaaccaat cttcttcttg atcagctcgg ctgtcggctc ggagatttgc atgccgtaat
    14821 tgcggcgaat gtagtcgatg atggactggt cgaacttgtc accgccgacg cgctcggact
    14881 tggcgtaaac gatgccgccc agcgaaatca cgccaacctc agtggtaccg ccaccgatgt
    14941 cgaccaccag cgaaccggtt gcctccgcca ccggcatgtc agcaccaata gcggcggcca
    15001 tcggttcctc gatcaggaac acctggcgcg cacccgccat ttcagcggat tccttgatgg
    15061 cacggcgctc aacctgggtc gaaccgaccg gcacgcagat gatgatgcgc ggactgggcg
    15121 agaacaggcg ggattcatgc acgcgacgga tgaaatactt gagcatctgc tcggtgatgg
    15181 tgaagtcggc aatgacacca tccttcattg gccggatcgc ttggatgttg gtaggcgcac
    15241 gccccaacat acgcttggct tcggcaccca cggcgaggat ggtcttcttg ttggtggggc
    15301 cgccttcgtg gcgcaccgcg acgacggaag gctcatccag cacaatgcct ttcccgcgca
    15361 cataaatcag agtgttggca gtaccaaggt caatggcgag atcgttggag aaataactgt
    15421 ttaagaaacc gaacatgtgt ttatatcccg agcatacttg ccaagccgga tggccttggc
    15481 tgaaggtgtg aattaaatcg cgctataata cccgatatcg ccttgaagtt taagacttac
    15541 agcttaatac catgtccctg accaccgacg acgtaaaacg cattgctcac ctcgcccgca
    15601 ttgaagtcac cgaagacgac gcgcaagcca cgctcagcaa actcagtggc attctgggcc
    15661 tgatcgaaca gatgcaggcg gtggacacca ccggcatcgc ccccatgtca cactcgcagg
    15721 acgtgtcgca acggttgcgc gacgatgtgg tcatcgaaac caaccagcgc gaactgttcc
    15781 agtccattgc gccggccgtc gaaaacggcc tctacctcgt tcccaaggtt attgagtaat
    15841 gattaacgca agtttgaagg aactggcggc tgcgctcgcc agcaagcaga tttcgagcgc
    15901 cgagctgacg caactgtttc tcgaccgcat ccgccggctc aacccggaaa tcaatgccta
    15961 tgtcacgctg gatgccgagc gcagcttggc ccaagcctcg gctgccgacg cactgattgc
    16021 gcagggcaag tttggcccgc tgaccggcat tccgttcgcg cagaaagaca ttttctgcgc
    16081 caagggctgg cgcaccacct gtggctcgaa gatgctggaa aacttcatcg cgccttacga
    16141 tgcccacatc atcagccagt tcgatgccgc cggcgccgtg aatctcggca aaaccaacat
    16201 ggacgagttc gccatgggct cctccaacga aacctcgtat ttcggcaagg tgcagaaccc
    16261 gtgggatcgc gcccgcgtac cgggtggcag ctcgggtggt tcggctgcgg ccgtggccgc
    16321 gcgcctggcg ccggcagcca ccggtaccga taccggcggc tcgattcgcc agccggcgtc
    16381 cctgtgcggc ctgtccggcc tcaagccgac ttacggtatc tgttcgcgct acggcatgat
    16441 cgccttcgcc tcgtccctgg atcaggccgg cccgatggcc aagtcggctg aagacctcgc
    16501 gctgatgctg aatgtgatgg ccggcttcga cagccgcgat tccaccagcc tgcagcgtga
    16561 aaaagaagat tacacccgcg acctgaacaa gccgctggcg ggccttcgca tcggcctgcc
    16621 cagggaatat tttgccgaag ggctggatgc gggtgtcgcc aaggtggtgc tggacgccat
    16681 cgaccagtac cgcaagctgg gcgctgaaac cgtcgaaatc agcctgccca acacccagct
    16741 ttccatcccc gtgtattacg tgctcgcgcc ggccgaagcg agttccaacc tgtcgcgcta
    16801 tgacggtgtg cgttacggcc atcgcgccgc ggaatacacc gacctgctcg acatgtacag
    16861 caagagccgc gccgaaggtt ttggcgcgga agtgaagcgc cgtatcctga tcggcaccta
    16921 tgtgctgagc gccagctact acgatgccta ctacctcaag gcccagcaac tgcgccgttt
    16981 gatcgcgcag gatttcgagc aggcgttcaa gcaatgtgac gtgatcatgg gcccggctgc
    17041 gcccggcacg gcattcaaga ccggcgaaaa aaccgatgac ccggtggcca tgtacctgga
    17101 agacctctat accattgccg tcaacctcgc cggtctgccg ggcatgagcg tgcctgctgg
    17161 tttcacgaat ggtctgccgg tcggtttgca gatcatcggc aactatttcg acgaggcgcg
    17221 catgctcagc gtcggccatc aatatcagca agtgacggac tggcacacca gaatgccgga
    17281 gggtatctaa atgcagtggg aagttgttat cgggctggaa acgcataccc agctccaaac
    17341 caaaaccaag attttttccg gggcctccac ggcgttcggg gcggaaccca acacccaggc
    17401 tgacgcggta agcatcgctc tgcccggcgt gctgccggtg ctgaaccgcg aggcggtgcg
    17461 ctgcgcggtt aagtttggcc tcgcgatcga cgccgaaatc ggccgacgct cggtgttcgc
    17521 acgcaagaac tacttctacc ccgatttgcc caagggctac cagatcagcc agtacgagct
    17581 gccggtggta ggcagggggc atctgaccat ccaggttggc gatgaagaaa aaaccgtgcg
    17641 catcacgcgc gcccacctgg aagaagacgc cggcaaatcg ctgcacgagg atttccacgg
    17701 catgaccggc atcgacctca accgtgccgg caccccgctg ctggaaatcg tcaccgagcc
    17761 cgacatgcgc tccgctgccg aagctgtggc ctacgccaaa aaactgcacg aactggtgat
    17821 gtggatcggg atctgcgacg gcaacatgca ggaaggctcc ttccgctgcg acgccaatgt
    17881 ttcggtgcgc cccaaaggct ccgacaagct gggcacgcgc cgcgagatca agaatctcaa
    17941 ttcctttcgc ttcatgcagc aggccatcga atacgaaacc cgctggcaga tcgaaacctt
    18001 ggaggacggt ggcaccatcg agcaggcgac cgtgctgttc aacccggata ccggcgaaac
    18061 ccgcgccatg cgcagcaagg aggaagccaa cgactaccgc tacttccccg acccggacct
    18121 gctgccg
//
LOCUS       DAOMFA010000011        17817 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_225_length_17817_cov_5.889652, whole genome shotgun sequence.
ACCESSION   DAOMFA010000011 DAOMFA010000000
VERSION     DAOMFA010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 17817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17817
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_225_length_17817_cov_5.889652"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..465
                     /locus_tag="PKW44_01215"
     CDS             <1..465
                     /locus_tag="PKW44_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019922635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HQR50239.1"
                     /translation="ASGAYSLTHGGNDAQKTIGIIWLLLISAGAATTDVNTLPDWVIY
                     ACYGAIAWGTYLGGWRIVKTMGTKLTKLNSVSGSCASMGGAISLGLATLGGIPVSTTH
                     TITGAIVGVGAAHRTRNVRWGVAMNLVVAWVLTIPASGLIAAWFWWLGKQFL"
     gene            529..2631
                     /gene="glgX"
                     /locus_tag="PKW44_01220"
     CDS             529..2631
                     /gene="glgX"
                     /locus_tag="PKW44_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018508005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HQR50240.1"
                     /translation="MKCECNVMPGLSHPLGATVSAHGVNFCVYTKSATQVELLLFDDM
                     RDAVPARAIPLDSHIHRTDHYWHVFVSDIRPGQLYAYRVHGPFDPARGLHFDPEKLLL
                     DPYGQAVAVPDNYSRDAASREGDNAARAMKSVVVDRRGYDWEGDRPLQLPFSQTLIYE
                     VHVGGFTRHPSSGVAPEKRGTYAGLAEKIPYLQQLGITAVELLPVFHFDPQDAPSGLV
                     NYWGYAPISFFAPHPAYSCSRDPLAQLNEFRDMVKALHKAGIEVILDVVFNHTAEGDQ
                     RGPTLCFRGFENSTYYMLERDRSRYANYSGTGNTLNADHPVVRRLIQDSLRYWVQEFH
                     VDGFRFDLASILARDSTGAPLANPPLLWDIETDPVLAGTKLIAEAWDAGGLYQVGSFI
                     GDSWQEWNGRFRDDVRDFFRGEPGSVGRFASRLLGSPDIYGHEQREAEQSINFVTCHD
                     GFTLNDLVSYDRKHNEANGEQNRDGANDNRSWNCGAEGPTDDPEMERLRNRQARNLLT
                     ATILSLGVPMILMGDEVRRTQGGNNNAYCQDNEISWFDWTLLEKNAGLLRFVQLLAAR
                     RVRRDTEAEQQRMSLNQLLRNTKLAWHGVKLHQPDWSDNSHSVALTTGIAKDAPLVHI
                     IFNAYWKPLEFELPPLDGKTRWRRWIDTALDSPDDIVEFGQAPPVDSFVYLAAARTTV
                     VLYAPMASTSGGSVPEVT"
     gene            complement(2668..2883)
                     /locus_tag="PKW44_01225"
     CDS             complement(2668..2883)
                     /locus_tag="PKW44_01225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR50241.1"
                     /translation="MPELLGKAVEARNVVLRMMASFNLFPPPPTQDPEQTLSFEFFYQ
                     RRAAFGRRDHGASSFKTELDSREIKNH"
     gene            complement(2951..3484)
                     /locus_tag="PKW44_01230"
     CDS             complement(2951..3484)
                     /locus_tag="PKW44_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HQR50242.1"
                     /translation="MPDRSLYLSRMHALDTLLCLSFNRTSRYRWVRLLFRLVSRLGDG
                     MFWCILMLGILMTEHAAGVLPVLHMATTGVCGTLVYKIVKGRTLRPRPFEVNQTVKVG
                     MAPLDRFSFPSGHTLHAVLFSVIALAYYPALAWLLVPFTLLVAASRVVLGLHYPSDVL
                     AGASLGALLATGSLALA"
     gene            complement(3737..4897)
                     /locus_tag="PKW44_01235"
     CDS             complement(3737..4897)
                     /locus_tag="PKW44_01235"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQR50243.1"
                     /translation="MNILFLSDVYFPRINGVSTSIHTLRGQLVEMGHRVHLVGPDYFS
                     PSDDESWITRVPSRYLPFDPEDRLMRYAETLNICAGRLRNDFDLIHVHTPFAAHYLGL
                     RLAKRYGIPCVETYHTFFEDYLHHYLPVLPRPLLRIAARSLSRRQCNTMDAVVAPSQP
                     MLDALRGYGVNVRVEVIPTGLEDHSFVAGDGAGFRQRYGIPLDRPVLLFVGRVAHEKN
                     IGFLLRMMAQLRQARPDALLVIAGEGPALEAVRGEALALGLQDNIRFLGYLDRKTELN
                     DCYRAADVFVFSSCTETQGLVLLEAMAQGVPVVAIAEMGTKSILREGEGALIAPLDEE
                     AFAARVKQLLGDPALRKRLGLVASGYARQWSAASMAERMVEFYRDVVQPKAG"
     gene            5033..5782
                     /locus_tag="PKW44_01240"
     CDS             5033..5782
                     /locus_tag="PKW44_01240"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020164088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HQR50244.1"
                     /translation="MRVRSIFVSDIHLGTRGCQAGRLLDFLREYESEHLFLIGDIVDF
                     WAMRRSIQWTATQNTVVQKVLRRARHGGKVVLIPGNHDEALREYDNTSFGDILIAGEW
                     VHDTADGRRFLLVHGDLYDQVTRYHRWVAVLGDAAYDFLVRANDWLSLIRRTLRIPGY
                     WSLAGYAKRKVKTAVNFICDFEDSVIHAVRERGLDGVICGHIHSAIIKQVERLTYVNC
                     GDWVDSCTAIIEHVDGRLELIGWGMTATSSP"
     gene            5908..8286
                     /locus_tag="PKW44_01245"
     CDS             5908..8286
                     /locus_tag="PKW44_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018078662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HQR50245.1"
                     /translation="MNTSQSAPVLSDAELHAINAYWRACNYLAAGMIYLRDNPLLREP
                     LRPEHLKNRLLGHWGASPGLAFTYIHMNRLINKYDLDAIFLAGPGHGAPGVLGPVYLE
                     GTYSEVYPNKGEDEEGLLQFFKEFSFPGGIGSHCTPETPGSIHEGGELGYSVSHAFGA
                     AYDNPDLIVTVMVGDGESETGPLATSWHANKFLNPLRDGAVLPVLHLNGYKINNPTLL
                     SRISHEELEHLFQGYGWKPYFVEGSDPLEMHAKMAATMEQCVLEIRRIQGEARNNGKP
                     VRARWPMIVLRSPKGWTGPKEVDGHKVEGFWRAHQVPLAGVKENPAHLAQLEAWLRSY
                     KAEELFDATGKLRPELKALAPKGSRRMSANPHANGGLLRRALRMPDFRDYAEQLPGPG
                     KNTAENTRPLGKLLRDIMAANMHNFRVFGPDENSSNKLDNVYEVSKKTWMADLLPEDA
                     DGGELSPDCRVMEMRSEHTLKGWLEGYLLTGRHGFFSTYEAFVHVIDSMFNQHAKWLA
                     MSREIPWRAPVSSLNLLITSTVWRQDHNGFTHQDPGFLDLVVNKSPEVTRIYLPPDVN
                     CLLSVANHCLNSTDYINVIVSDKQKHLQYLDMDAAIKHCTKGIGIWEWASNDAGVEPD
                     VVMASAGDIPTKEALAATVLLREHFPALKVRFVNVVDLYKLTPASEHPHGLSDRDFDS
                     LFTADKPVIFNFHGYPWLIHRLAYRRTNHKNFHVRGYKEKGSINTPLELAIQNEIDRF
                     SLAIDVIDRVPALRIAGAHVKEQLRDKQIECRNYAYEHGTDLPEADGWTWPY"
     gene            8349..9434
                     /locus_tag="PKW44_01250"
     CDS             8349..9434
                     /locus_tag="PKW44_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019899288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HQR50246.1"
                     /translation="MNILTINSGSSSLKASLFLAEGRRRDFRYGHIGHGFPHDHAEAF
                     DALLGELGNDRPAAVGHRFVHGGEITDAALLVDDAERARLEDISYLAPLHMPGNLLGV
                     DLCRARFDVPQVACFDTAFHATLPELARRLPIPSEFGLRRYGFHGINYAHVARRLPQL
                     IGDTAFGRVVIAHLGSGASLCLLQNLQSVDTSMGYTPAGGIPMGTRSGDLDPGVMLEL
                     AKHRDEAELSHLVYHRMGLLALSDGESNEMSALLQSGSDAARFAVEYFARQVRAAIGG
                     YAAKCGGIDVLVFTGGIGEHAAKVRALICGPLGFLGFALDAEANSRHATTLSTPASKP
                     VLVVPADEEGEIAALTGALLQGEKVSR"
     gene            9516..10961
                     /gene="ccoG"
                     /locus_tag="PKW44_01255"
     CDS             9516..10961
                     /gene="ccoG"
                     /locus_tag="PKW44_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HQR50247.1"
                     /translation="MSTSTEDTDNEAPMYQKRIPIHTRTVKGKFRNLKTGILLLAYAV
                     YFILPWLPWARPSGAQQAILFDMVSRRFFIFDLVIYPQDIFLLSLLLFIAAALLFFTT
                     GLIGRAWCGYFCFQTLWTDAFILIEHIIQGERPARLRLQKQPWNMEKILKIGGARALM
                     LLISFWTAFTFVAYFNHTPEFLFRFFSGDSVSTAYYAVTVLTISTFLAGALAREQVCI
                     YMCPYARFQSVMYEADTLVPAYNATRGEGSTGRIVAKSGVHTLEERHAKGHGDCIDCG
                     YCVQVCPTGVDIRKGLQYQCISCGLCIDACNTIMDSLGWERGLIRYDSERNLSSPAPA
                     KPHLDFMRLKVLGYAVALTLMTGVLVYKLASRTEFEVRIAQVRQPLYVMLSDEKIRNR
                     YEIHIVNKSQNDELYLIQAEGIPKNALNLGAIPNPAPVAAGKDIKIPVKVDLDEQTAE
                     KTKGFDYLVIPQSSPDKKETRHASFDSPKRH"
     gene            complement(11025..11393)
                     /locus_tag="PKW44_01260"
     CDS             complement(11025..11393)
                     /locus_tag="PKW44_01260"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - diacylglycerol kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HQR50248.1"
                     /translation="MKSPHKGQAGLRRLINAFGYSMHGFRDAYEHEHAFRQEVMLAAV
                     LLPLAFLLEGSGLGRALMVGSVLLVVLVELLNSAIEATVDRISLETHVLAKRAKDIGS
                     AAVLLSLINLMVVWGLVLFG"
     gene            complement(11438..12796)
                     /gene="glmM"
                     /locus_tag="PKW44_01265"
     CDS             complement(11438..12796)
                     /gene="glmM"
                     /locus_tag="PKW44_01265"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HQR50249.1"
                     /translation="MSRKYFGTDGVRGRVGETPITPDFVMRLGYAAGRVLTGMDSHLA
                     PGAHPAVLIGKDTRISGYMLESALQAGLSAAGVDILLTGPMPTPAVAYLTRALRAQAG
                     IVISASHNPFEDNGIKFFSAEGTKLPDAIEHAIEAELDQPMRVMPSAQLGKARRIDDA
                     AGRYVEFCKSTFPNALDLRGLKIVLDCAHGATYHVAPDVFHELGADVIAIGHQPNGLN
                     INHDCGSTHPGVLQQEVVRYGADLGIAFDGDGDRVVMVDGAGNLLDGDQLLYIIAMQR
                     QRAGKLHGGVVGTLMTNLALEQALAKHGIAFARAKVGDRYVLELMNEKGWLLGGENSG
                     HILCLDKHSSGDGIIAALQVLHAIRESGVSLQTLASDLTLFPQVLVNVVVKQRIDLDG
                     NAAIREAVRSAESELNGSGRVLLRPSGTEPKVRVMVEGRKRPQVEALAQRIADTVRLA
                     AA"
     gene            complement(12793..13620)
                     /gene="folP"
                     /locus_tag="PKW44_01270"
     CDS             complement(12793..13620)
                     /gene="folP"
                     /locus_tag="PKW44_01270"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019897731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HQR50250.1"
                     /translation="MIFRCGSRTLDLSCPRVMGIVNATPDSFSDGGRFAATEAAIAHG
                     LQLVAQGADILDIGGESTRPGASPVALDEELRRVLPVVEGLAEKAGVPISIDTYKPEV
                     MRAAVAAGADIVNDINALQAPGTLEAVAQTNAGVCLMHMQGTPQTMQADPQYHDVVNE
                     VADFLRGRMAAAEAYGIAAERIVLDPGFGFGKRSVHNIALLRELETLCALRRPLLVGL
                     SRKSVLGQIVGADVDARLHASLSAAVIAAMKGARILRVHDVKATVDALRVVSVVEGL"
     gene            complement(13672..15564)
                     /gene="ftsH"
                     /locus_tag="PKW44_01275"
     CDS             complement(13672..15564)
                     /gene="ftsH"
                     /locus_tag="PKW44_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HQR50251.1"
                     /translation="MNNIVKNIAIWLIVALVLMTVFNHFGVKQAAESQVGYSQFLNEV
                     KQGHIAKVVIDGHVLRGETKDGKKFNTYAPSDLWLVSDLLERNVIVEAKPDEEQSLLM
                     NIFVSWFPMLLLIGVWVFFMRQMQGGGKGGAFSFGKSKARLLDENNNQITFADVAGCD
                     EAKEEVGELVEFLRDPSKFQKLGGRIPRGVLMVGNPGTGKTLLAKAIAGEAKVPFFSI
                     SGSDFVEMFVGVGAARVRDMFEQAKKNAPCIIFIDEIDAVGRQRGAGLGGGNDEREQT
                     LNQLLVEMDGFEGSVGVIVIAATNRPDVLDPALLRPGRFDRQVVVPLPDIRGREQILM
                     VHMRKVPIAPDVKADIIARGTPGFSGADLANLVNEAALFAARGNKRLVDMEDFERAKD
                     KIMMGAERRSMVMSEEEKRNTAYHESGHAVVAKLLPKADPVHKVTIIPRGRALGVTMQ
                     LPEEDRYAYDREFLMNRIAIMFGGRIAEELFMNQMTTGASNDFERATQLARDMVTRYG
                     MSDALGPMVYGENEGEVFLGRSVTTHKNLSEDTMRKVDAEIRRIIDQQYGLARKLLED
                     NRDKVEAMTAALLEWETIDAEQIGDIMAGNPPRPPKPSQSTPTPPRDSGPSAAAPTTA
                     KPAQET"
     gene            complement(15618..16238)
                     /locus_tag="PKW44_01280"
     CDS             complement(15618..16238)
                     /locus_tag="PKW44_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HQR50252.1"
                     /translation="MKRSRTSKAWMQEHVSDEFVKRAQRDGYRARAAYKLMEVDDRDK
                     LFKPGMVVVDLGSAPGSWSQVAVQRMHGKGRVIALDLLEMQPMPGVEFLQGDFREEAV
                     LKELEKMLAGREVDLVISDMAPNISGISSTDQARMAHLAELALEFSLQWLKPGGHLLV
                     KVFAGSGFDDIVHAMRQGFEQVVTRKPKASRDRSSEVYVLALRRRP"
     gene            16274..16570
                     /gene="yhbY"
                     /locus_tag="PKW44_01285"
     CDS             16274..16570
                     /gene="yhbY"
                     /locus_tag="PKW44_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020182553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HQR50253.1"
                     /translation="MPQLNSKQTSHLRALSHNLNPVVMVGNNGLTEQVLREIEVSLNA
                     HELIKIKVFGDDRELRKALLETICEKTGAAPIHHIGKQLVIYRPADKPKIILPK"
     gene            complement(16577..16903)
                     /gene="ftsB"
                     /locus_tag="PKW44_01290"
     CDS             complement(16577..16903)
                     /gene="ftsB"
                     /locus_tag="PKW44_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HQR50254.1"
                     /translation="MRVLTLILVILIAALQYPLWLGKGSWLRVWDLSRQIEKQKDGNA
                     TLKARNDALDAEVRDLKTGYAAIEERARSELGMIKQDEVFYQVMDGSMSPPPKPMEST
                     PNPVVP"
     gene            complement(16921..>17817)
                     /gene="eno"
                     /locus_tag="PKW44_01295"
     CDS             complement(16921..>17817)
                     /gene="eno"
                     /locus_tag="PKW44_01295"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HQR50255.1"
                     /translation="LYRYLVGAGRMQLTVPMMNIINGGAHASNSIDMQEFMVIPAGRP
                     SFREAVRCGAEIFHALKKILNKKGLTTTVGDEGGFAPDLPTNETALQYIMEAISAAGY
                     EPGGDVLIGVDCASSEFYKDGLYHIEAEGLQLNSGQFADYLSTWVDKYPIISIEDGMS
                     EFDWDGWSLLTQRMGDSIQLVGDDLFVTNAKILREGIQRGVANSILIKVNQIGTLTET
                     FDAIETAKRAGYTSVISHRSGETEDTTIADIAVATNALQIKTGSLSRSERIAKYNQLL
                     RIEEELGEGSYPGRDAYYQLIG"
BASE COUNT         3645 a         5690 c         5053 g         3429 t
ORIGIN      
        1 gcttccggcg cctacagcct gacccacggc ggcaacgatg cacaaaaaac tatcggcatc
       61 atctggctgc tgctcatcag cgccggcgct gccaccaccg acgtcaacac cctgccggac
      121 tgggtaatct acgcctgcta tggcgccatt gcatggggga cttacctcgg cggctggcgc
      181 atcgtcaaga ctatgggcac caagctgacc aagctcaatt ccgttagtgg ctcctgcgcc
      241 tcaatgggcg gtgccatcag cttggggctg gccacgctgg gcggtatccc ggtgtccacc
      301 acgcacacca tcaccggcgc cattgtcggc gtcggtgccg cccaccgcac ccgcaacgtg
      361 cgctggggcg tcgcgatgaa ccttgtcgtg gcttgggtgc tgaccatccc cgccagcggc
      421 ctcatcgccg cctggttctg gtggctgggg aaacagtttc tgtaggcgac atccccttta
      481 gcatgggggc tcgcaaggcg gatgatcaga gcctgatcgc catagcatat gaaatgtgaa
      541 tgcaacgtca tgccgggcct gagtcatccg ctcggcgcca cggttagcgc gcacggagtc
      601 aatttctgcg tgtacaccaa aagcgccacg caggtcgagt tattgctgtt cgacgatatg
      661 cgcgatgctg taccggcacg cgccatcccg cttgactcac atatccaccg caccgatcac
      721 tactggcatg tgttcgtgtc cgacatccgg ccgggacagc tttacgctta ccgcgtccat
      781 ggccctttcg atcccgctcg aggcctacat ttcgatccgg aaaaactgtt gctcgaccct
      841 tatggccaag cggttgccgt gcctgataat tacagccgcg acgccgcatc gagggaaggc
      901 gacaacgcgg ctcgcgcgat gaaaagcgtc gtcgtggacc gacgcggcta tgactgggag
      961 ggtgaccgcc ccttgcagtt gcctttttcc cagaccctga tttacgaagt gcatgtaggc
     1021 ggcttcacgc gccaccccag ttccggagtc gctccggaga aacgcggcac ctatgccggc
     1081 ctcgccgaaa agatccccta cctgcagcaa ctcggcatca cggcggtaga attattgccg
     1141 gtgtttcatt tcgacccgca ggatgccccg tcaggcctgg tcaattactg gggctacgcc
     1201 ccgatctcct tcttcgcgcc ccatccggcc tatagctgta gccgcgaccc cctcgcccag
     1261 ctgaacgagt tccgtgacat ggtcaaggct ttgcacaagg ccgggatcga agtcattctg
     1321 gatgtcgtct tcaaccacac tgcagaaggc gaccaacgcg gtccgacgct gtgttttcgc
     1381 gggtttgaga acagcaccta ctacatgctg gagcgagacc ggtcacgcta tgccaattac
     1441 agcggcaccg gcaacacgct caacgcggat catcccgtcg tccgcaggct gattcaggac
     1501 agcctgcgct actgggtcca ggagttccat gtcgacggct ttcgtttcga cctagcgtcg
     1561 atcctcgccc gggattccac gggagcgcca cttgcgaatc cgccgctgct gtgggatatc
     1621 gaaaccgatc ctgttctcgc cggcaccaag ctgatcgccg aagcatggga tgctggcggc
     1681 ctctaccagg tcggcagctt cattggcgac agctggcagg aatggaacgg ccgcttccgc
     1741 gacgatgtgc gcgacttttt ccgaggcgaa cccggcagcg tcggccgctt cgccagccgc
     1801 ctgctcggca gcccggacat ctacggccat gaacagcggg aagcggaaca gagcatcaac
     1861 ttcgtcacct gccatgacgg ctttacgctg aatgacctcg tctcctacga ccgcaagcat
     1921 aacgaagcca atggcgagca gaatcgcgac ggcgcgaacg acaaccgcag ctggaattgc
     1981 ggcgcggaag gaccgaccga tgatcccgaa atggagcgtc tgcgcaaccg gcaggccaga
     2041 aatctgctga ccgcaaccat actctcactc ggtgtgccga tgatcctgat gggcgacgaa
     2101 gtcaggcgca cgcaaggggg caacaacaac gcctactgcc aggacaacga aatcagctgg
     2161 ttcgactgga cgctactgga aaaaaacgcc ggcctgctcc gcttcgttca gctgctcgcg
     2221 gcccgccgcg ttcggcgtga taccgaagcc gagcagcaaa gaatgagtct gaatcaactg
     2281 ctccgcaata ccaagctcgc gtggcatggc gtaaaactcc accagcccga ctggagcgac
     2341 aattcacata gtgttgcctt gaccactggc atagccaagg acgccccgct ggtccacatt
     2401 attttcaacg cgtactggaa gccgctggag ttcgagctcc cgccgctcga tggcaagacc
     2461 cgctggcggc gctggatcga cactgcgctg gattcccccg acgatatcgt agaattcggc
     2521 caggcgccgc cagtcgatag ctttgtgtat cttgcggcgg cgcgaacgac tgtcgtgctg
     2581 tacgcgccga tggcatcgac ttctggaggc tccgtcccgg aggtaacgta gggtggcagt
     2641 ggcgtggcac gattctgtag ccaaccttca atggttcttg atctcccggc tatccagttc
     2701 ggttttaaag ctgcttgcac catggtcacg ccggccaaat gccgccctgc gctgataaaa
     2761 gaattcaaag gacagggttt gttcaggatc ctgcgtgggc ggcgggggaa agagattaaa
     2821 tgaagccatc atgcgcagca ccacgttcct tgcctcgaca gccttgccca acagttccgg
     2881 cacggaattg ctcatcgcac tactcctctc agatcgtctt tgaagtactt tgccaatgca
     2941 atggctgcaa ctaggccagc gccagggagc ccgtggcaag cagggcaccc aggctggcgc
     3001 cagccaatac gtcactgggg taatgcagcc ccagcaccac cctggaagcg gcgaccagca
     3061 gcgtaaacgg caccagcagc caagccagtg ccgggtagta agccagcgca atcacgctga
     3121 acagcacggc atgcagcgta tggccggaag ggaaactgaa acgatccagc ggcgccatgc
     3181 cgacttttac ggtctggttg acctcgaacg ggcgaggacg cagtgtccgt cccttgacga
     3241 ttttgtagac cagcgtcccg caaacgcccg tcgtcgccat atgcagcacc ggcagtacgc
     3301 cagcggcgtg ctcggtcatg aggatgccaa gcatgagaat gcaccagaac atgccatcgc
     3361 ccaatcggct taccaggcga aacagcagac gaacccagcg ataacggctg gtgcgattga
     3421 agctgaggca gagcagcgta tccagcgcgt gcatgcggct gaggtaaaga gagcgatcag
     3481 gcatctggcg gctcctgtag tattgtgtgt gaatacccct tcatctggcc gtaaaccgtt
     3541 tcccgggaat actggcccgt tgcagcgccg aactcccggc caagcgggaa agtgtcattg
     3601 ccaatgtctg gaaaccgctg cggagtgctt gccatggcgt aagattccca tgcaaggatg
     3661 acggcgcagt gacgtttttg cgaacaagcg atgacagcgt tgccaacact acaggagcca
     3721 gggtcatcta ggccaatcat ccagctttgg gctggaccac gtcacggtag aattccacca
     3781 tcctctccgc catgctggct gccgaccatt gcctggcgta gccagaggca accagcccca
     3841 ggcgcttacg caaagccgga tcgccaagca gttgtttcac tctggcggca aatgcctcct
     3901 cgtccagcgg cgcaatcagg gcgccctcgc cttcgcgcag gatggatttg gtacccattt
     3961 cggcaattgc caccaccggc acaccctgcg ccatcgcttc cagcaacacc agcccttggg
     4021 tttcagtgca cgatgaaaac acgaaaacat ccgccgcacg atagcagtcg ttgagttcgg
     4081 tcttgcgatc cagatagcca aggaagcgga tattgtcctg caagcccagg gccagtgcct
     4141 cgccgcgcac cgcttccagt gccgggcctt cgcccgcgat caccagcaag gcatcggggc
     4201 gcgcctgcct gagctgcgcc atcatgcgca gcaggaagcc gatgtttttc tcgtgggcga
     4261 cacgcccgac gaacaaaagt accggccggt cgagcgggat gccatagcgc tgacggaatc
     4321 cagcgccatc tccggcgacg aaactgtgat cttccagccc ggtgggaatg acctcgaccc
     4381 gcacattcac cccgtagccg cgcagcgcat ccagcatggg ctgcgagggc gccaccacag
     4441 catccatggt attgcactgc ctgcgcgaca ggctgcgtgc ggctatcctg agcagcggcc
     4501 tgggcagaac gggtaggtag tgatgcagat agtcctcgaa gaaggtgtgg taggtttcca
     4561 cgcagggaat gccatacctt ttggccagtc gtaaccccag gtaatgcgcg gcgaaaggtg
     4621 tatgcacgtg gatcaggtcg aaatcgttgc gcagccgacc ggcacatatg tttagcgtct
     4681 ctgcatagcg catcaggcgg tcttccgggt cgaacggcag gtagcgcgac ggcacgcgcg
     4741 tgatccagct ttcgtcgtca ctcgggctga aataatctgg tcccaccagg tgcacgcggt
     4801 gccccatctc caccagttga ccacgcaggg tatggatgga agtagagacg ccgttgatac
     4861 gggggaagta gacatcggac agaaacagga tgttcacgca tggcctttcg tgatgaagtc
     4921 cggcacatgc tagggagtgc ttgtgacatt ccgatgaaac gtcttgctgc tttcatgcaa
     4981 acgtcacaca aagtctctag gcttggcaag ccgttaattc gatacctgag taatgagagt
     5041 tcgctccatt tttgtttccg acatccatct cggcacacgc ggctgccagg ccgggcgcct
     5101 gctcgatttc ctgcgcgagt atgaaagcga acacctgttc ctgatcggtg acatcgtcga
     5161 tttctgggcc atgcggcgca gcatccagtg gacggcaacg cagaacaccg tggtgcagaa
     5221 agtcctgcgc cgcgcccgcc acggcgggaa agtggtattg attccgggca accatgacga
     5281 ggcgctgcgc gaatacgaca acacctcgtt cggcgatatc ctgattgcgg gcgagtgggt
     5341 ccacgacacc gcggacggac ggcgcttcct gctggtgcat ggcgacctct atgaccaggt
     5401 tacccgctac caccgctggg tggcggtact gggcgacgcc gcctatgatt ttctggtcag
     5461 agccaacgac tggctgtcgc tgatacgccg caccctgcgc atcccgggtt actggtcgct
     5521 ggcgggctac gccaagcgca aggtcaagac cgccgtcaac ttcatctgcg acttcgagga
     5581 ttccgttatc cacgccgtac gcgaacgcgg gctggacggc gtcatttgcg gccacatcca
     5641 cagcgccatc atcaagcagg tggagcgctt gacctatgtc aactgcggcg actgggtaga
     5701 tagctgtacg gccatcatcg agcacgtgga cggacggctg gaactgatcg gatggggcat
     5761 gacggcgacg tcaagccctt agttagactt atcgcgccga gagggcgcgc ctgcggaaag
     5821 gctgccagta ccgtcggcaa atccagacat ggctctccac caataatgtt aaaattctct
     5881 atcccctaac gcgaaggaac ccctcaaatg aacacctcgc agtccgcacc ggtactttcc
     5941 gacgccgaac tccacgccat caacgcctac tggcgcgcct gcaactacct ggcggccggc
     6001 atgatctacc tgcgcgacaa cccgctgctg cgcgagccgc tcaggccgga acacctcaag
     6061 aaccgcctgc tgggacactg gggcgccagt cccggcctcg cctttaccta catccacatg
     6121 aaccgcctga tcaacaagta cgaccttgac gccatctttc tggccggccc cggccacggc
     6181 gcacccggag tgctgggccc ggtctacctg gaagggacgt atagcgaggt ctaccccaac
     6241 aagggcgaag acgaggaagg cctgctgcag ttcttcaagg aattctcctt ccccggcggc
     6301 atcggcagcc actgcacgcc ggaaacgcca ggctccatcc acgagggcgg agaactcggc
     6361 tacagcgtca gccatgcctt cggcgcggcc tacgacaacc ctgatctgat cgtcaccgtg
     6421 atggtgggcg acggcgaatc cgaaaccggg ccgctggcca cttcctggca tgccaacaaa
     6481 ttcctcaacc cgctgcgcga tggcgctgtg ctgccagtgc tgcacctcaa cggctacaag
     6541 atcaataacc ccaccctgct ctcccgcatt tcgcacgagg aactggagca cctgttccag
     6601 ggctacggct ggaaacctta tttcgtcgag ggcagcgacc cgctggagat gcacgccaaa
     6661 atggctgcta ccatggagca atgcgtgctg gaaatccgtc gcatccaagg tgaggctcgc
     6721 aacaacggca agcccgtccg cgcgcgctgg ccgatgatcg tgctgcgctc gcccaagggc
     6781 tggaccggtc caaaagaggt cgatggtcac aaggtggaag gcttctggcg cgcccaccag
     6841 gtgccgcttg ccggtgtcaa ggaaaacccc gcccatctgg cgcagctgga agcctggctg
     6901 cgcagctaca aagcggaaga attgttcgat gcgaccggca aactgcgtcc ggaactgaag
     6961 gcgctggccc cgaaaggcag ccgccgcatg agcgccaacc cgcacgccaa cggcggcttg
     7021 ctgcggcgcg cgctgcgcat gccggatttc cgcgactacg cggagcaact tcccggaccc
     7081 ggcaagaata cggcggaaaa cacgcgtccg ctcggcaagc tgctgcgcga catcatggcc
     7141 gccaacatgc acaatttccg cgtgttcggc ccggatgaaa acagctccaa caagctggac
     7201 aatgtctacg aagtcagcaa gaaaacctgg atggctgatc tgctaccgga ggatgccgat
     7261 ggcggcgagt tgtctcccga ttgccgcgtt atggaaatgc gctccgagca tacgctgaag
     7321 ggctggctgg agggctacct gctgaccggc cgccatggct tcttctctac ctatgaagcc
     7381 ttcgtgcacg tgatcgactc catgttcaac cagcacgcaa aatggctggc gatgtcgcgt
     7441 gaaatcccgt ggcgcgcacc ggtttcctcg ctcaacctgc tcatcacctc cactgtctgg
     7501 cgccaggacc acaacggctt tacccaccag gatcccggct tcctcgacct tgtggtcaac
     7561 aagagtcccg aagttacccg tatctacctc ccgcccgacg tcaactgcct gctgtcggtg
     7621 gccaaccact gcctgaacag caccgactac atcaacgtca ttgtcagcga caagcagaag
     7681 catttgcagt atctggacat ggacgccgcc atcaagcact gcaccaaggg catcggcatc
     7741 tgggaatggg ccagcaacga tgccggtgta gagccggacg tggtgatggc aagcgcgggc
     7801 gacatcccga ccaaggaggc actggctgcc accgtgctac tacgcgaaca tttccccgca
     7861 ctgaaggtcc gtttcgtcaa cgtggtcgac ctctacaagc tgacgccagc atccgagcac
     7921 ccgcacggcc tgagcgaccg tgatttcgac agcctgttca ccgcggataa gccggtgatc
     7981 ttcaacttcc acggctaccc ttggctgatt caccgtctcg cctaccgccg caccaatcac
     8041 aagaatttcc acgtgcgcgg ctacaaggaa aagggcagta tcaatacccc gctggaactg
     8101 gcgatccaga acgagatcga ccgcttcagc ctggccatcg acgtcatcga ccgcgttccc
     8161 gcgctgcgca tcgccggcgc ccacgtcaag gaacagttgc gcgacaagca gattgaatgc
     8221 cgcaactatg cctatgagca cgggactgat ttgccggaag cagatggctg gacctggccg
     8281 tattgatact agggagtggg gagtggggga aaccaccccc acccccgact tctcactccc
     8341 cacgttccat gaacattctc accatcaaca gcggttcttc cagtctcaag gccagcctgt
     8401 tcctggccga gggccgccgc cgcgatttcc gctacggcca tattggccac ggcttcccac
     8461 acgaccacgc cgaggctttc gacgccttgc tgggcgaact gggtaatgac cggccggctg
     8521 cggtcggcca tcgttttgtg catggcgggg aaatcaccga tgccgcactc ctggtagacg
     8581 acgcggaacg ggcgcgcctg gaagatatct cctacctcgc cccgttgcac atgccaggca
     8641 atttgctggg cgtggacctg tgccgggcac gtttcgacgt gcctcaggtc gcctgtttcg
     8701 acactgcgtt ccacgccact ctgccggaac tcgcccgacg cctgccgatt ccaagcgaat
     8761 tcggcctgcg ccgctacggc ttccacggca tcaactacgc ccacgtcgcc cgccggctgc
     8821 cgcaactgat cggcgatacc gctttcggcc gggtggtcat cgcccacctc ggcagcggcg
     8881 ccagcctgtg tctgctgcag aatctgcaat ccgtggacac cagcatgggc tacactcccg
     8941 ccggcggtat cccgatggga acgcgcagcg gcgacctcga ccccggcgtg atgctggaac
     9001 tggccaagca ccgtgatgaa gcggaactct cgcatctggt ctatcatcgc atgggactgc
     9061 tggcgttatc ggacggcgag agcaacgaaa tgtcggcctt gctgcaaagc ggcagcgacg
     9121 ccgcccgctt cgccgtcgag tacttcgccc gccaggtacg cgccgccatc ggcggctatg
     9181 cggcaaaatg cggcggcatc gatgtgctgg tattcaccgg cggcatcggc gaacacgccg
     9241 ccaaagtgcg cgccctcatc tgcggcccac tgggttttct cggctttgcg ctggatgcgg
     9301 aggctaatag ccgtcacgcg acaactttga gcacgcctgc ctccaagccg gtgctggtcg
     9361 tgcccgctga cgaggaaggg gaaatcgccg cactcactgg cgcactcttg caaggagaaa
     9421 aagtctcacg ataatggcca acaggccggc ctgtatatta gttatttctg atatactgtt
     9481 ttcatttcat cgcctttgcc agaagggata acgcagtgag tacctcgacc gaagacaccg
     9541 acaacgaagc tccgatgtac cagaagcgga tcccgatcca tacccgcacc gtcaagggca
     9601 agttccgcaa cctgaaaacc ggcatcctgc tactggccta cgcggtttac tttatcctgc
     9661 cctggctgcc ttgggcacgc cccagcggcg cacagcaggc gatcctgttc gacatggtgt
     9721 cacgccgctt cttcattttc gatctggtga tctacccgca ggatatcttc ctgctatccc
     9781 ttctgctgtt catcgcagct gccttgctgt tcttcaccac cgggctgatc ggccgggcgt
     9841 ggtgcggcta cttctgcttc cagactttgt ggacggatgc cttcatcctg atcgagcaca
     9901 tcattcaggg tgagcgcccg gcacgacttc ggctgcaaaa gcaaccctgg aatatggaaa
     9961 aaatcctgaa aatcggcggt gcgagagctc tgatgctgct gatttcgttc tggaccgcgt
    10021 ttaccttcgt cgcctatttc aaccatacgc ccgaattcct gttccgcttc ttctcgggag
    10081 attcggtttc caccgcctat tacgcagtca cggtgctgac catctcgacc ttcctggctg
    10141 gcgccttagc acgcgagcag gtatgcatct acatgtgccc ctacgcccgc ttccagagcg
    10201 tgatgtacga ggcagatacg ctagtgccgg cctacaacgc cacgcgtggc gaaggcagca
    10261 ccgggcgcat cgtcgccaaa agcggggtgc atactctgga ggagcgccac gccaagggac
    10321 atggcgactg catcgattgc ggctattgcg tacaggtttg cccgaccggg gtggacatcc
    10381 gcaagggcct gcaataccag tgcatctcct gcggattgtg catcgacgcc tgcaacacca
    10441 tcatggattc gctgggctgg gaacgcggcc tgatccgcta cgattcggaa cgcaacctga
    10501 gcagtccggc acccgccaag ccacacctcg atttcatgcg cctcaaggta ttgggctatg
    10561 ccgtggcgct gaccttgatg accggggtac tggtctacaa gctggcctcg cgtaccgagt
    10621 tcgaagttcg catcgcgcag gtgcggcagc cgctatacgt catgctgtcg gacgaaaaaa
    10681 ttcgcaaccg ctatgaaatt catatcgtca acaagagcca gaacgacgaa ctctacctga
    10741 ttcaggcgga aggcatcccg aaaaacgcac tcaacctggg cgccatcccc aacccggcac
    10801 ccgttgctgc cggcaaggac atcaagatac cggtcaaggt cgatctggac gagcagacag
    10861 cagaaaagac caagggattc gactatctgg tcattccgca gtcaagcccg gacaagaagg
    10921 aaacgcggca cgccagtttc gacagcccga agagacattg aagtagcgtt gtagaccttg
    10981 gcactgcagg aactgcgcgc ggaaggggag gtggtgctac ccgtctagcc gaacaacacc
    11041 agcccccata ccaccatcag attgatcagg ctaagcagca cggcagcact gccgatatcc
    11101 ttggcacgct tggccagcac atgggtttcc agcgaaatgc gatcgacggt ggcttcgatg
    11161 gcggaattca gcagttccac cagtaccacc agcagcacac tacccaccat cagcgcacgc
    11221 ccgaggccgc tgccttccag gagaaacgcc aacggcagca gcaccgccgc cagcatgacc
    11281 tcctggcgga aagcgtgttc atgctcgtag gcatccctga agccgtgcat cgaataaccg
    11341 aatgcgttaa tcaggcgcct gaggccagcc tggcctttat gggggctttt catggcaatc
    11401 atcctttgtg tttgttgtag tccgtgccac cacgggttca agccgcggca agtctgaccg
    11461 tatcggcaat tcgctgcgcc aatgcttcaa cctgggggcg cttacggcct tccaccatta
    11521 cccgtacctt gggttcggtg ccggaagggc gcagcagcac gcggccactc ccgttcaatt
    11581 cgctttcagc ggaccttacc gcctcccgga tcgcggcgtt gccatccaga tcgatgcgtt
    11641 gttttaccac cacattgacc agcacttgcg ggaagagcgt caggtcggaa gccagggtct
    11701 gcaacgacac gccgctttcg cggatcgcat gtaacacctg caatgcggcg atgatgccat
    11761 caccgctgct gtgcttgtcc aggcacagga tgtggccgga attctcaccg cccagtagcc
    11821 agcccttttc gttcatcaat tccagcacgt agcggtcgcc cactttggcg cgggcgaacg
    11881 cgatgccatg cttcgccagt gcctgctcca gtgccaggtt ggtcatcagc gtaccgacca
    11941 ccccgccgtg cagtttaccg gcacgctggc gctgcatggc gatgatatac agcaactggt
    12001 caccgtccag cagattgccg gcgccgtcca ccatcaccac gcggtcgccg tcgccatcga
    12061 aggcaatgcc gaggtcggcg ccgtaacgca ccacttcctg ctgcaggaca ccggggtgcg
    12121 tagagccgca atcatggttg atgttgagcc cattcggctg gtggccgatt gcgatgacat
    12181 cggcgccaag ttcgtggaat acatcaggcg caacgtgata agtcgcgccg tgcgcgcagt
    12241 ccagcacgat cttgaggccg cgcagatcaa gcgcattggg aaaggtgctc ttgcagaatt
    12301 cgacgtagcg cccggcggca tcgtcgatgc ggcgcgcctt gcccagttgc gcggaaggca
    12361 tgacccgcat gggctggtcc agttcggcct cgatggcatg ctcgatagcg tccggcaatt
    12421 ttgtgccctc ggcagagaag aacttgatgc cattgtcctc gaacgggtta tgcgaggcgg
    12481 aaatgacaat gccggcctgg gcgcgcagcg cacgtgtcag gtaagccact gccggggtcg
    12541 gcatcgggcc ggtaagcaga atatccacgc cggcagcgga aagcccggcc tgcaaggccg
    12601 attccagcat gtagccggag atgcgcgtat ccttgccgat cagcaccgcc gggtgcgcac
    12661 ccggcgccag gtggctgtcc atgccggtca gcacccgccc tgcggcataa cccagccgca
    12721 tcacgaaatc cggcgtaatc ggtgtttcgc cgacccgccc acgtacacca tccgttccaa
    12781 aatattttct gctcataatc cttccaccac cgaaacaacc ctgagcgcat cgaccgtcgc
    12841 cttgacgtca tgcacacgca ggattctagc gcctttcata gccgcaatca cggctgccga
    12901 caggctggca tgcaaacgcg cgtccacgtc agcgccgaca atctggccca gcacggattt
    12961 gcgcgacaac ccgaccagca gcggccgcct caacgcacac aaggtttcca gttcacgcag
    13021 caacgcaatg ttatgcacgc tgcgcttgcc gaagccaaag ccgggatcga gcacaatgcg
    13081 ctcggccgcg atgccatagg cctctgccgc cgccatgcgg ccacgcagaa aatccgccac
    13141 ctcgttcacc acgtcatggt actggggatc ggcctgcatg gtttgcggcg tgccctgcat
    13201 gtgcatcaaa cacacgcccg cattggtctg cgctacagcc tccagagtgc cgggagcctg
    13261 caacgcattg atgtcgttga cgatgtcagc gcccgctgcc acagcggcgc gcattacttc
    13321 cggcttgtag gtgtcgatag aaattggcac gccagccttt tcggccagcc cctcgaccac
    13381 cggaagtacg cgccgcaact cctcgtccag tgccactgga ctggcaccgg gacgggtcga
    13441 ctcgccaccg atgtcgagga tgtcggcgcc ctgcgccacc agttgcaatc catgcgcaat
    13501 ggccgcctcc gtcgccgcaa agcgccctcc gtcagaaaag gagtccggag tggcgttgac
    13561 gatgcccatg acgcgggggc aggaaaggtc gagggtgcgg ctgccgcagc ggaagatcat
    13621 tgggggaaag ggaaggggga agggagtttg cgccttttac ccttcgctgt ttcaagtttc
    13681 ctgtgccggc ttggcggtgg tcggtgccgc cgccgaaggt ccgctatcgc gcggcggcgt
    13741 gggcgtgctc tggctcggct tgggtggacg cggcgggttg ccggccatga tatctccgat
    13801 ctgctcggca tcgatggttt cccattccag cagggctgcg gtcatggctt ctaccttgtc
    13861 gcggttgtct tccagcagct tgcgggcgag accgtactgt tggtcaatga tgcggcggat
    13921 ttccgcatcc accttccgca tggtgtcttc ggacaagttc ttgtgcgtgg tcacggaacg
    13981 gccgaggaac acctcgccct cgttttcgcc atacaccatc ggccccagcg cgtcggacat
    14041 gccgtagcgc gtcaccatat ccctggctaa ctgcgtggcg cgctcgaaat cgttggaggc
    14101 gccagtcgtc atctggttca tgaacagttc ttccgcgata cggccgccga acatgatggc
    14161 gatgcggttc atcaggaact cacggtcgta ggcgtagcgg tcttcctccg gcaactgcat
    14221 ggtcacgccc agcgcgcggc cgcgtggaat gatggtgacc ttgtgcaccg gatcggcctt
    14281 gggcagcagc ttggcgacaa ccgcgtggcc ggattcgtga taggcggtat tgcgcttttc
    14341 ttcctcgctc atcaccatgg aacggcgctc ggcgcccatc atgatcttgt ccttggcgcg
    14401 ttcgaagtct tccatgtcca ccaggcgctt gttgccgcgt gcggcaaaca gcgcagcctc
    14461 attgaccagg ttggcaagat cggcgccgga gaaccctggc gtaccacgcg cgatgatgtc
    14521 ggccttgacg tccggggcaa tcggcacctt gcgcatatgc accatgagaa tttgctcgcg
    14581 gccacggatg tccggtagcg gaacgaccac ctggcgatcg aaacggccag ggcgtaacag
    14641 tgctggatcg agcacgtccg ggcggttggt ggcagcgatc actatcacgc cgacgctgcc
    14701 ttcgaagcca tccatttcca ccagcaattg gttcagcgtc tgctcgcgtt cgtcgttgcc
    14761 accaccgagg ccggcgccgc gctggcggcc gactgcgtcg atttcgtcga tgaaaataat
    14821 gcacggtgcg tttttcttgg cctgttcgaa catgtcgcgc acacgtgcgg caccaacacc
    14881 aacgaacatt tcgacgaaat cggagccgga aatcgagaag aacggcacct tggcctcgcc
    14941 tgcgatggct ttggccagca aggtcttgcc ggtaccggga ttgcccacca tcagcacgcc
    15001 tcgcggaatc cgcccaccca gcttctggaa cttggaagga tcccgcagga actcgaccaa
    15061 ttcaccgacc tcctccttgg cctcgtcgca accagcgaca tcggcgaaag tgatctgatt
    15121 gttgttctcg tccagcaaac gcgccttgct cttgccgaag gagaaggcgc cgcccttgcc
    15181 accgccctgc atctggcgca tgaagaacac ccagacgccg atcagcaaca gcatggggaa
    15241 ccaggagacg aaaatattca tcagcagcga ctgctcttca tcgggcttgg cctcgacgat
    15301 gacatttctt tccagcaggt cggaaaccag ccacaagtcg gatggggcat aagtgttgaa
    15361 tttcttgccg tccttggttt cgccgcgcaa cacatggccg tcgatgacca ctttggcaat
    15421 atgcccctgt ttcacctcat tcagaaactg ggaatagcca acctggctct cagccgcctg
    15481 cttgacgccg aaatgattga aaaccgtcat aagcaccagc gcaacgatca gccagatggc
    15541 gatgttcttt acaatattgt tcaaaatccc gttccttgtg ttaaaggaaa aatgtgcggt
    15601 tccattctaa accgatttca tggcctgcgt cgcaatgcca gaacatagac ttcactgctc
    15661 ctgtcgcgag aagccttggg cttgcgcgtc accacctgct cgaagccctg cctcatggca
    15721 tgcacaatat catcgaaacc actaccggca aaaactttga ccagcaagtg cccgcccggt
    15781 ttcagccatt gcagtgaaaa ttccagcgcc agctcagcca gatgcgccat gcgtgcctga
    15841 tcggtgctgc tgatgccact gatattgggg gccatatcgg aaatcacaag atcgacttcc
    15901 cgccccgcca gcattttttc cagctccttg agcaccgcct cctcgcggaa atcgccttgc
    15961 agaaattcca cccccggcat cggctgcatt tccagcaaat ccagtgcaat cacgcggccc
    16021 ttgccatgca tccgctgcac cgccacctgc gaccagctgc ctggcgcgga gcccagatcg
    16081 accaccacca tgccaggctt gaacagcttg tcgcgatcat cgacctccat cagcttgtag
    16141 gcggcgcgcg cgcggtagcc gtcacgctgg gcacgcttga cgaactcgtc actcacgtgc
    16201 tcctgcatcc aggccttgct ggttctgctt ctcttcatgt ggtagactgc gcgccttttc
    16261 gaatcaacga cctatgccac aactcaactc aaaacaaacc agccacctgc gcgccctcag
    16321 ccacaacctg aatccggtgg tcatggttgg caataacggc ctgaccgagc aggtgctgcg
    16381 cgaaatcgaa gtcagcctga atgcgcacga actcatcaag atcaaggtat tcggcgacga
    16441 ccgcgaactg cgcaaggctc tgctggaaac catctgtgag aaaaccggcg ccgcccccat
    16501 ccatcacatc ggcaagcaac tggtcatcta ccgccccgca gacaagccga aaatcatcct
    16561 gccgaaatag tcagagtcag ggtactaccg ggttgggcgt ggactccatc ggcttgggcg
    16621 gcggactcat gctgccgtcc atcacctgat agaacacttc gtcctgtttg atcatgccca
    16681 gttcactacg ggcacgttcc tcgatcgcag catatcccgt tttgagatcg cgcacttcag
    16741 catccagcgc gtcgttgcgc gccttgagcg tcgcgttacc gtctttctgc ttttcgatct
    16801 gccggctcag gtcccacacc cgcaaccaac tgcccttgcc aagccacaaa ggatattgca
    16861 gtgccgcaat gagaatgacc aggatcagcg tgagcaccct catggcagcg atggcggcaa
    16921 tcaaccaatc agctggtagt aggcgtcgcg ccccgggtag ctgccttcac ccagttcttc
    16981 ctcgatacgc agcaactggt tgtacttggc gatgcgctcg gagcgcgata gcgaacccgt
    17041 cttgatctgc agcgcgttgg tcgccactgc gatgtcggcg atggtggtgt cttccgtctc
    17101 gcctgagcgg tgtgaaatca ccgaggtgta gccagcgcgc ttggcggttt cgatggcatc
    17161 gaaggtctcg gtcagcgtac cgatctgatt gaccttgatc aggatggaat tggccacgcc
    17221 gcgctggatg ccctcgcgca ggatcttcgc gttggtcacg aacaggtcgt cgccgaccag
    17281 ctggatcgaa tcgcccatgc gctgtgtcaa cagactccaa ccgtcccagt cgaactcgct
    17341 catgccgtcc tcgatactga tgatgggata cttgtccacc caggtagaga gataatcggc
    17401 gaactgcccg gaattgagct gcaaaccctc tgcttcgata tggtacaggc catccttgta
    17461 gaactcggaa ctggcacaat cgaccccgat cagcacgtcg ccgcccggtt cgtacccggc
    17521 agcggaaatc gcttccatga tgtattgcag cgccgtctcg ttggtcggca ggtcgggggc
    17581 gaagccacct tcatcgccca cggtagtggt caggcccttc ttgttcagaa ttttcttcaa
    17641 cgcgtggaag atttccgcac cacagcgtac cgcctcacga aaactcggcc gccccgccgg
    17701 gatcaccatg aattcctgca tatcgatgct gttggaagca tgtgcaccac cgttgatgat
    17761 attcatcatc ggcaccgtca gttgcatgcg accggcaccg acgagataac gatatag
//
LOCUS       DAOMFA010000012        17499 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_232_length_17499_cov_5.499427, whole genome shotgun sequence.
ACCESSION   DAOMFA010000012 DAOMFA010000000
VERSION     DAOMFA010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 17499)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17499)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17499
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_232_length_17499_cov_5.499427"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1760
                     /locus_tag="PKW44_01300"
     CDS             <1..1760
                     /locus_tag="PKW44_01300"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HQR50256.1"
                     /translation="LDTVREKNSSLLHQAMPEMENLQSQMIGTQSMVILTDAHGTILH
                     ALGDDDFITKAQRVALQPGVSWSEENTGTNAIGTALVEQAPVFVMGAEHYFSQNAFLN
                     CSASPILDPHGQTIGVLDVSGDYRQPQAHTMALVRMSAQMIENRLFSAEFARDITLHF
                     HMRPEFIGTLWEGIAVFSLDGRLLAINRCGNAQLGLSHIEAGCLAFDDLFDSTLVSLL
                     SAARHRLPHGTPLRTQSGRQICVKVEPGISLSARTGNDRPREIQAPNRGTALNHDLLE
                     RLDTGDQQMRSVIGRIKKVLLRDIPILIEGETGTGKELLAKAVHQASGRKGMFVAINC
                     ASIPEGLIEAELFGYEEGAFTGAKRSGVPGKIMQANGGTLFLDEIGEMPLPLQARLLR
                     VLQEREIVPLGSAKGTPVDIAVISATNRKLRERVEAGDFREDLYYRLNGLRVGLPPLR
                     ERNDLDGLVSRILREECDSPVSLHRETATLFSRHPWRGNVRQLRNILRTALAFVDEDH
                     VIRIPHLPEDFIEELHTSRPASFPPQPKNMARIADAETDLIRQALKDHAGNMSQAAKH
                     LGISRATLYRKVQKLKQSR"
     gene            1943..2419
                     /locus_tag="PKW44_01305"
     CDS             1943..2419
                     /locus_tag="PKW44_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit gamma"
                     /protein_id="HQR50257.1"
                     /translation="MTLPDRTEKAIQAAISSNKDKPGALLPILHNIQDDLGHIPPEAV
                     PVIAGELNLSRAEVHGVISFYHYFRQTPPGRHTLQICRAESCQAMGSEALTERAKQCL
                     GIDFHGTTSDQEFSLEPVYCLGNCACSPAVMLDDEVYGRVTPERLEQLLAQARRGQ"
     gene            2419..3972
                     /locus_tag="PKW44_01310"
     CDS             2419..3972
                     /locus_tag="PKW44_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018411751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HQR50258.1"
                     /translation="MSIKVYVPRDSSALSLGANKVAMTIVVEAAKRGASIEIVRNGSR
                     GLFWLEPLVEVATPQGRIAYGPVNPADVPSLFECNFLYGGEHHLRLGPTEDIPYLKNQ
                     ERLTFARVGITDPVSLQDYLAHDGYRGLKHALGMDGAAIVKEVTDSGLRGRGGAAFPT
                     GIKWNTVLNTAADQKYIVCNADEGDSGTYSDRMIMEGDPFVLIEGMTIAGIAVGAMQG
                     YIYLRSEYPHALQVLNEAIAITTANNYLGEDILGSGKTFHLEVRRGAGAYVCGEETSL
                     LESLEGKRGIVRFKPPLPAIEGLFGKPTIINNVISLASVPIILGKGGAFYRDYGMGRS
                     RGTLPIQLAGNLKHCGLVEKAFGATLRELLYDYGGGSATGRPIRAVQVGGPLGAYLPE
                     SQFDTPLDYEAFSNIWAVLGHGGIVAFDDTADMAKMARYAMEFCAIESCGKCTPCRIG
                     STRGVEVMDKIIADKDRAANIALLRDLNDTMLNGSLCAMGGMTPYPVLSALNHFPRDF
                     GLQQKQQAA"
     gene            3984..6851
                     /gene="fdhF"
                     /locus_tag="PKW44_01315"
     CDS             3984..6851
                     /gene="fdhF"
                     /locus_tag="PKW44_01315"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020163649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HQR50259.1"
                     /translation="MACQCGQGGCDTDYGTPARVSEQLVTLEIDGIEVSVPQGTSLMR
                     AAAEAGIQVPKLCATDSLEPFGSCRLCLVEVEGRRGYPASCTTPADNGMKVRTQTDKL
                     ADIRRGVMELYISDHPLDCLTCAANGNCELQDMAGAVGLREVRYGYDGENHLKDIKDE
                     SNPYFTYDPSKCIVCNRCVRACEETQGTFALTIDGRGFESRVSAGQTEPFLESECVSC
                     GACVQACPTATLTEKSVIQFGQAEHSITTTCAYCGVGCSFKAEMQGNRVVRMVPDKNG
                     QANHGHSCVKGRFAWGYATHPDRITKPMIRDNISDPWKEVSWQEAVDFAARRFREIQA
                     KHGRASVGSITSSRCTNEETYLVQKLVRAAFGNNNVDTCARVCHSPTGYGLKQTLGES
                     AGTQTFDSVMQADVVMVIGANPTDGHPVFGSQLKRRLREGAKLIVVDPRAIDLVQSPH
                     IKADYHLKLRPGTNVAIANALAYVIVTEGLADDAFAAARCDTREYAAWKEFISRPHHS
                     PEALQAETGVPAELVRGAARLYASANNAAIYYGLGVTEHSQGSTMVMAIANLAMATGN
                     IGRLGVGVNPLRGQNNVQGSCDMGSFPHELPGYRHVSDSAARGLFEAAWDVKLDHEPG
                     LRIPNMFEAALDGSYKGIYVEGEDIAQSDPNTQHVTAALEAMECVVVQDLFLNETAKF
                     AHVFFPGASFLEKDGTFTNAERRISPVRTVMPPLAGKADWEVTQMLSNALGYPMNYNH
                     PSEIMDEIARLTPTFHGVSYARIDELGSIQWPCNEQAPDGTPIMHVEQFVRGKGKFMF
                     TEYVPTDEKVTHKFPLILTTGRILAHYNVGAQTRRTANTAWHPEDILEIHPHDAEERG
                     IADGDWVGIQSRAGETVLHARISIRMQPGVVYTTFHHPFSGANVITTDNSDWATNCPE
                     YKVTAVQVVKVTQPSEWQKRYRQFSEHQQELLQQRAVVKTQ"
     gene            6852..7691
                     /gene="fdhD"
                     /locus_tag="PKW44_01320"
     CDS             6852..7691
                     /gene="fdhD"
                     /locus_tag="PKW44_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HQR50260.1"
                     /translation="MSTAPLSCEPPMEAEFASHTRRTVKRWRGGAWVHAIDQVAEEVP
                     VAMVYNGISHAVMLATPVGLEAFGLGFSLSEGILHGRGELYDLEVVRRREGIELQMTI
                     ATERFAELKQRRRNLAGRTGCGLCGTESLQQVFRHPQPVPGGDLHMTPQAIHAVFREL
                     VPTQTLQSLTGAAHAAAWVSRQGELLHVEEDIGRHNALDKLIGALAAKSLPFDQGCAI
                     VTSRASYEMVQKAATVGIPLLAAISAPTGLAIQLAEHCGVTLLGFVRRDSHVVYAHPQ
                     RLK"
     gene            7706..7927
                     /locus_tag="PKW44_01325"
     CDS             7706..7927
                     /locus_tag="PKW44_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018230136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit delta"
                     /protein_id="HQR50261.1"
                     /translation="MDIHHLVKMANQIGQFFESEPDQQQAVQDIATHIKRFWDPRMRK
                     TLIGHIENGGAGLLPIVMKSLQENQESLV"
     gene            8019..8651
                     /locus_tag="PKW44_01330"
     CDS             8019..8651
                     /locus_tag="PKW44_01330"
                     /inference="COORDINATES: protein motif:HMM:NF013460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HQR50262.1"
                     /translation="MKIVLVWRWPVRLMHWGMVAAFSVAMWYRDSIMDQLFHVYAGYA
                     MAAILFARIIYGFAARDLAAFRRFPPAPIKGAKYAFDLIRGKARNYLGHNPAGALAIY
                     GMLLLGLATVGTGYWAFEYDNELAKTWHHHFAYTWFWLICLHLFGVLMGSLAHKEFLV
                     WAMITGCKTRRSISQPFSLQAVGITLLIIFLHLLNLIVILFGGKGFINRK"
     gene            complement(8694..9338)
                     /locus_tag="PKW44_01335"
     CDS             complement(8694..9338)
                     /locus_tag="PKW44_01335"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQR50263.1"
                     /translation="MTPLLSIEGLRKAYGDRTLLDIKCLELQRGTSYLLMGDNGVGKT
                     TLLRILAGLERAQAGNFTFDGVRYDEAPARARLAPRMIYVHQHSYVFNTSVADNIGYG
                     LSGLPKSEREARVRVEMAWANLEHLAEVPPYRLSGGEKQRVALARARVLKPELLLLDE
                     PTANLDDAAREQVAELIRHMRDANNCVLIATHDHDLMKLEDVVKMRLEQGRVTA"
     gene            complement(9335..10030)
                     /locus_tag="PKW44_01340"
     CDS             complement(9335..10030)
                     /locus_tag="PKW44_01340"
                     /inference="COORDINATES: protein motif:HMM:NF038017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQR50264.1"
                     /translation="MGLTEATLQAFRWLLEGDLTIWGIVWVSLRVALLALLAVTPLAI
                     VAGFGLATLQFPGRRALIVLMQTLLALPTVVVGLIVYLLLTRNGPMGSFELLFTQQGM
                     VIGQALLAFPILTAMTLSAVQGIDARVSETARTLGASPLRASFTVLLEARFAVMAAIA
                     TGFGRVLSEIGCAMMVGGNIAGVTRNITTAIALETSKGEFAQGIALGVVLVALALGVN
                     FGIAFLQGKGEIK"
     gene            complement(10036..10854)
                     /locus_tag="PKW44_01345"
     CDS             complement(10036..10854)
                     /locus_tag="PKW44_01345"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HQR50265.1"
                     /translation="MIKRTMQLWAVVVFSALISFPAGAGSSKAIRLATTTSTANTGLL
                     DYLLPKFEVKCGCKVNVIAVGTGKALKLGEDGNVDVVLVHARPAEDAFVAAGYGVDRR
                     DVMYNDFVLVGPNNDPARIRGEKDVLAAMKKIADAKWKFVSRGDDSGTDQMEKSYWKA
                     LDINPAGLPWYFSAGQGMGEVLMMSGEMRAYTLTDRGTWIAYREKIGLPMLVQGDPRM
                     FNPYGIIAVNPKKYPSANYQGAKALTDWITSAEGQKLIADFKVGGEQLFIPDAK"
     gene            complement(10870..12468)
                     /locus_tag="PKW44_01350"
     CDS             complement(10870..12468)
                     /locus_tag="PKW44_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HQR50266.1"
                     /translation="MHQAIGGNKRKLAALVLAAVSSGAYAEELLDPAVRIQQLEKIMQ
                     QMQVQREEQNKQLQVMAEELKAMQQQMANGKEEKVKEKGKSTGSPVYAAFKDGLVFDD
                     GSGNWKLQLNGRVQADYRDYNPSEWQSDTFSIRRARFGGTFTFLKDYSIRVEGEYANV
                     PSASATSYNNNSSATTGLTYAYLNYGHWKEANIRLGQFKPFFGLERAESTNFTDFTEL
                     SLATNNGTIFNSTYDRGVMVFGDPVPGVNYNIYAVNGTGMNANDFNNQKDTGARINAN
                     FAQLADIKNAVLHVGTSASTGNLGFDTSCSSGPNPCSNTGLISQQTEGMGVKFFSTTN
                     GMHGNSDRTRLGFETALTYGPVKLQSEYIHANFDGKWNSGSSTYHYDNDINNWYVDLN
                     WMLTGESYADNYKSGVFGRMSPKNNLTFGKDGWGAIEFGLRYSKFDASDFANKPSGSS
                     VSMLANTTGTKGCGSSNPAAVSTNTCYTSQADAWTTGVKWILNPNARIMLNYIHTSFD
                     DPVTFNGRTQDFENDLVLRTQFDF"
     gene            complement(12616..12969)
                     /locus_tag="PKW44_01355"
     CDS             complement(12616..12969)
                     /locus_tag="PKW44_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HQR50267.1"
                     /translation="MSSSNIKIRIPYGTLSAMGPGKADLLDAIESSGSISAAARNMGM
                     SYKRAWDLVDTMNRCFRQPLVSTATGGSHGGGAQVTEFGHEVRHRYRALVAKAEQSVA
                     AELQELVGMLAPNKR"
     gene            13092..14102
                     /gene="moaA"
                     /locus_tag="PKW44_01360"
     CDS             13092..14102
                     /gene="moaA"
                     /locus_tag="PKW44_01360"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019898573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HQR50268.1"
                     /translation="MGFIDSNSAVLIDRFGRRVDYIRLSITDRCDFRCVYCMGEDMTF
                     LPREQVLTLEECERLVRVFVELGVRKVRITGGEPLVRRDALWLFQHIGRLSGLDELTL
                     TTNGSQLEQYAGQLKDAGVDRINISLDSLDAERFRKVTRIGNIHKVLRGIEAARAAGF
                     AHTKLNTVLMHGFNDDEAPQLVQFAIDKQLDIAFIEEMPLGEVDHLRASSYVSNEETL
                     KTLQGHFSLVSSAETTGGPARYWRVPGTSTRIGFISPHSHNFCESCNRVRITCKGELY
                     LCLGQEDKVDLMPYLRDHPDDDAPLRNAILNSMEIKPKGHDFDLRRAEPAVVRFMSMT
                     GG"
     gene            14105..14938
                     /locus_tag="PKW44_01365"
     CDS             14105..14938
                     /locus_tag="PKW44_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019917098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HQR50269.1"
                     /translation="MSRLRPQITHASRPLTFTVPSIDEHGEAGTTEIAGEGPLTLYLD
                     KREIVTLMTLGAAPEALAIGYLRNQRLLPDIEEIAAVQVDWETDAVAVTTRNGIANIE
                     QRLGKRTVTTGCGQGTMFGDLMEELDQLRLPHDATLDEATLQALLDNVRHYDSLYKKA
                     GALHGCALGQGAEILYYVEDVGRHNAVDAIAGMMWLDDVSGHDKTFYTTGRLTSEMVI
                     KAAQMGIPFLVSRSGLTRMGWEIAQKVGITMIGRAQGRHFLLFTGAERFRHTEKAQAA
                     A"
     gene            14935..15504
                     /gene="mobA"
                     /locus_tag="PKW44_01370"
     CDS             14935..15504
                     /gene="mobA"
                     /locus_tag="PKW44_01370"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HQR50270.1"
                     /translation="MNVTGIVLAGGMGRRMGGVDKGLLPFHGKPLVAHVLERLQPQVT
                     EILLNANREIEAYQAFGYPVISDAIGGFAGPLAGLHSGMSVAKTPLVVTVPCDSPFLP
                     ADLAQRLLQAMEKHQADLAVAKTGDQPHPVFCLCRTSLLPGLTRFLESGGRKIDAWYA
                     GLNAVEVPFDDEADAFANFNTPAELEAAA"
     gene            15501..16061
                     /gene="mobB"
                     /locus_tag="PKW44_01375"
     CDS             15501..16061
                     /gene="mobB"
                     /locus_tag="PKW44_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008062768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HQR50271.1"
                     /translation="MIRCEVPLLGFCAWGSGVGKTTLLTSLIPVLTLHGLRISVVKHA
                     HHTFDIDHPGKDSYRLRESGAIQTLLGSRQRWALMTELSHIEPQRQEPSLVELLTHMD
                     TTLADLILVEGFKQEAIPKIEVYRPSLGTPLLADQDPHVIAVAADGSAPTKLPLLDLN
                     DPVAIGEFVVAWLKQQRTTLKIVSTH"
     gene            16074..17342
                     /locus_tag="PKW44_01380"
     CDS             16074..17342
                     /locus_tag="PKW44_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HQR50272.1"
                     /translation="MTTDFIKAASCADDYDPNSMPVDKARKYIRDYLTPVQSKECLPL
                     RSALGRVLAEDILSPCNVPNHNNSAMDGYALNADDLAATGATTLRIAGTAFAGESFAG
                     KMGKGECVRIMTGAVMPEGCDSVVIQEHVQTEGDRISFGEGVKRGQNLRYAGEDLKLG
                     QTVFSAGHLVRPADLGLIASLGIGEVRVYRKLRVAFFSTGDELASIGQPLQEGQVYDS
                     NRYTLYGMLARLGVEIIDMGVVRDDPGLLEQALLDASAAADVILTSGGVSVGEADFMK
                     QLLNDLGQVVFWKIAMKPGRPLAYGKIANAHYFGLPGNPVSVMVTFYQFVRDALLVLM
                     GQPNPAPIPLLNATCTEALKKAPGRTEFQRGSLFLDADNSWKVRPIGNQGSGVLRSMS
                     EANCFIVLADDCGNVAAGATVQAQLLEGLV"
BASE COUNT         3588 a         5532 c         5092 g         3287 t
ORIGIN      
        1 agctggacac ggtcagggag aaaaactcta gcttgctgca tcaagccatg ccggaaatgg
       61 agaatctgca atcacagatg atcggcaccc agagcatggt gatcctgact gacgcacacg
      121 gcacgattct gcacgcactg ggcgatgacg atttcatcac caaggcgcag cgcgtagcgt
      181 tgcaaccggg cgtttcctgg agcgaagaaa acaccggcac caatgcgatt ggaaccgcgc
      241 tggtcgagca ggcgccagtg tttgtcatgg gtgccgagca ctatttcagc cagaacgctt
      301 ttctgaactg ctccgcctca cccattcttg atccacacgg ccagaccatc ggggtattgg
      361 acgtttccgg cgattaccgc cagccgcagg cgcacaccat ggcgctggtg cgcatgtcag
      421 cacagatgat cgagaatcgc ctgttcagcg ccgagttcgc acgcgacatt accttgcatt
      481 tccatatgcg ccccgagttc atcggcaccc tgtgggaagg cattgccgtg ttttcactgg
      541 acggccgcct gctggccatc aatcggtgtg gtaacgccca gcttggcctg agccacatcg
      601 aggcgggatg cctggcattt gacgacctgt tcgatagcac tttggtatcc ttgctcagtg
      661 ctgccaggca ccggctaccc catggcacgc cgcttaggac tcagtccgga cggcagatct
      721 gcgtcaaggt agagccaggc atttccctca gcgcccggac tggcaacgac aggcccaggg
      781 aaatacaggc cccaaaccgc ggaacagcac taaaccacga cttgctggaa cgtctggata
      841 ccggcgacca gcaaatgcgc agtgtgattg gccgtatcaa aaaagtcctg ctgcgtgaca
      901 ttcccatcct gatcgaggga gagaccggca ccggcaagga actactggcg aaggccgtcc
      961 accaggcgtc gggccgcaag ggcatgttcg tcgccatcaa ttgcgcgtcc atcccggaag
     1021 gcttgatcga agccgagctt ttcggttacg aagaaggcgc tttcaccggc gctaagcgca
     1081 gcggggtgcc cggtaaaatc atgcaggcca atggcggtac gctgtttctc gatgaaatcg
     1141 gcgaaatgcc tttgcccttg caggccagat tgctgcgcgt gctgcaagag cgcgagatcg
     1201 tgccgctggg tagcgcaaag ggtacacccg tggacatcgc cgtcatttct gcgaccaacc
     1261 gaaaattacg cgagcgcgtc gaagccggcg acttccggga agacctttac tatcgcttga
     1321 acggcctgcg cgtgggcctg ccgccgctac gcgaacgcaa tgacctggat ggcctggttt
     1381 cccggatact gcgagaagaa tgcgacagcc cagtctcgct gcatcgcgag acggccaccc
     1441 tgttcagccg gcacccatgg cgcggaaatg tgcggcagct caggaatatc ttgcgtaccg
     1501 ccctggcatt tgtcgatgaa gaccatgtca tcaggatacc gcacctcccg gaagacttca
     1561 tcgaggaact ccacaccagc agaccggctt ctttcccgcc ccagccaaaa aacatggctc
     1621 gcatcgcaga tgcggaaact gacctgatca ggcaggcgct gaaagatcac gcaggcaata
     1681 tgtcgcaagc ggcaaagcac cttggcatca gccgtgccac cttgtatcgc aaggtccaaa
     1741 agctgaaaca atcccggtaa aatcgcccgc cacgttcaac cccttttctc gcacccaata
     1801 aaaccacact gccaagatcg ttgacgatat tggctttaag gctatattct tgtgcgtata
     1861 acgttttgcc cctgatgggc catttttttg cggatcgata tatacgacag gatatagaga
     1921 tagcgcagca agcgaggaaa tcatgaccct gccagacagg acagaaaagg cgatccaggc
     1981 agcgatctcc agcaacaagg acaagccagg cgcactactg ccgatactgc acaacattca
     2041 ggatgatctc ggccacatcc cgcccgaagc cgtgccggtg atcgccggtg aactcaatct
     2101 gtcgcgcgcc gaggtgcatg gcgtcattag cttctatcat tactttcgcc agactccgcc
     2161 gggacggcac accctgcaaa tctgccgggc cgagtcctgc caggccatgg gcagcgaggc
     2221 gctgaccgag cgcgcaaaac aatgtctggg catagatttt cacggcacca ccagcgacca
     2281 ggaattctcc ttggagccgg tttactgcct aggcaattgc gcctgctccc cggctgtcat
     2341 gctggacgac gaggtttacg gccgcgtcac cccggaacga cttgaacaac tgctggccca
     2401 ggcgaggaga gggcaataat gagcatcaag gtctatgtcc cgcgcgactc cagcgcgctt
     2461 tcactgggcg ccaacaaagt ggcgatgacc atcgtggtcg aggccgccaa gcgtggcgcc
     2521 agcatcgaga tcgtgcgcaa cggctcgcgc ggcctgttct ggctggaacc tctggtcgag
     2581 gtcgccacgc cgcagggacg catcgcctac ggcccggtca atccagcaga cgtgcctagc
     2641 ctgttcgaat gcaattttct ctatggcggc gagcaccatt tgcggctggg gccgacggaa
     2701 gacatccctt acctgaaaaa tcaggagcgg ctgaccttcg cccgcgttgg catcaccgat
     2761 ccggtttcgt tgcaggacta cctggcccat gacggctacc ggggcctcaa gcatgcattg
     2821 ggcatggatg gcgccgccat cgtcaaagag gtgaccgatt ccggcctacg cgggcgcggc
     2881 ggcgcggcct tccccaccgg catcaagtgg aatacggttc tgaacacggc ggccgaccag
     2941 aaatacatcg tatgcaatgc cgacgagggt gattccggca cctactccga ccgcatgatt
     3001 atggaaggcg acccgttcgt gctgatcgaa ggcatgacca tcgccggcat cgcggtgggc
     3061 gccatgcagg gctacatcta cctgcgctcg gaatacccgc atgccctgca agtgctgaac
     3121 gaggccatcg ccataaccac tgccaataac tatctgggcg aagatattct gggcagcggc
     3181 aaaactttcc acctcgaagt gcggcgcggc gccggggctt acgtctgcgg cgaggaaacc
     3241 tcgctactgg aaagcctgga aggcaagcgc ggcatcgtgc gcttcaagcc gccgctgccg
     3301 gccatcgaag gcctgttcgg caagcccacc attatcaaca acgtcatctc gctggcctcg
     3361 gtgcccatca tcctgggcaa gggcggcgcg ttctaccgcg actatggcat gggccgctcg
     3421 cgcggcacgc tgccgatcca gctagccggc aacctcaagc attgcgggct ggtagaaaag
     3481 gccttcggcg ccacgctaag agagttgctg tatgactacg gcggcggttc cgccactggc
     3541 aggcccatcc gcgcggtgca ggtcggcggc ccgctcggcg cctatctgcc ggaatcgcag
     3601 ttcgacaccc cgctggatta tgaagccttc agcaatatct gggcggtgct cggccacggc
     3661 ggcatcgtgg cgttcgacga tacggcggat atggcgaaaa tggcgcgcta cgccatggaa
     3721 ttctgcgcca tcgaatcctg cggcaagtgc acgccctgcc gcatcggttc gacgcgcggg
     3781 gtggaggtca tggacaagat catcgccgac aaggatcgtg ccgccaacat cgccttgttg
     3841 cgcgacctca acgacaccat gctcaacggc tcgctgtgcg caatgggcgg catgacaccc
     3901 tacccggtgc tgtcggcgct caaccatttc ccgcgggatt tcggcctgca acaaaagcag
     3961 caggctgcgt aaggagaaac atcatggcat gtcaatgcgg tcaaggcggg tgcgacaccg
     4021 actacggcac cccggcaaga gtttccgaac aactggtgac actggaaatc gacggcatcg
     4081 aggtgagcgt gccgcaaggt acctcgctga tgcgcgcggc ggcggaagcg ggcatccagg
     4141 ttcccaagct gtgtgccacc gacagcctgg agccgttcgg ctcctgccgc ctgtgtctcg
     4201 tcgaggtgga aggacggcgc ggctatccgg catcctgcac cacccccgcc gacaacggga
     4261 tgaaagtacg cacgcagacc gacaagctgg ccgacatccg gcgcggggtg atggaactgt
     4321 atatctccga ccacccactg gactgcctga cctgcgccgc caacggcaac tgcgagttgc
     4381 aggacatggc cggcgcagtc ggcctgcgcg aggtgcgcta cggctacgat ggcgaaaacc
     4441 acctcaagga catcaaggac gaatccaatc cctattttac ctacgacccc agcaagtgca
     4501 tcgtctgcaa ccgctgcgtg cgcgcctgcg aggaaaccca gggcacgttc gcgctgacca
     4561 tcgacgggcg cggcttcgaa tcgcgcgtgt cggcggggca aacggagccc ttcttggaat
     4621 cggaatgcgt ctcctgcggc gcctgcgtgc aggcctgccc gaccgccacc ctgacggaaa
     4681 aatccgtcat ccagttcggc caagccgaac acagcatcac caccacctgc gcctattgcg
     4741 gcgtgggctg ctcgttcaag gccgagatgc agggcaaccg cgtggtacgc atggtgccgg
     4801 acaagaatgg ccaggccaac cacggacact cttgcgtcaa gggccgcttc gcctggggct
     4861 atgccaccca cccggatcgc atcaccaagc cgatgatccg agacaacatc agcgacccat
     4921 ggaaagaggt gagctggcag gaggcagtag atttcgccgc gcgccgcttc cgcgaaatcc
     4981 aggccaaaca cggccgcgca tcggtaggca gcatcacctc ctcgcgctgc accaacgagg
     5041 agacctatct ggtgcagaaa ctggtgcgcg ccgccttcgg caacaacaat gtcgacactt
     5101 gcgcccgcgt ctgccattcg ccgactggct atgggctgaa acagaccttg ggcgaatccg
     5161 ccggcaccca gaccttcgac tcggtgatgc aggccgatgt cgtcatggtg atcggcgcca
     5221 atcccaccga cggccacccg gtattcggct cgcagctcaa gcgccgcctg cgtgaaggcg
     5281 ccaagttgat cgtggtcgat ccgcgtgcca tcgacctcgt gcaatcgccg catatcaagg
     5341 cggattacca tctcaagctg cgtcctggca ccaacgtggc catcgccaac gccttggcct
     5401 acgtcatcgt caccgaaggg ctggccgacg atgcctttgc cgccgcgcgc tgcgacacgc
     5461 gcgaatatgc ggcatggaaa gagttcatct cgcgcccgca ccactctccg gaagcgctgc
     5521 aagccgaaac cggcgtgccg gcggagctgg tgcgtggcgc cgctcgcctg tacgcgagcg
     5581 ccaataacgc cgccatctac tacggcctgg gcgtgaccga gcacagccaa ggctcgacta
     5641 tggtcatggc tatcgccaac ctcgccatgg ccaccggcaa catcggccgg ctgggtgtgg
     5701 gtgtcaaccc gctacgtggg caaaacaacg tgcagggctc ctgcgacatg ggctccttcc
     5761 cgcacgaact gccaggttac cgccatgttt ccgacagtgc cgcgcgcggc ctgttcgaag
     5821 cggcgtggga cgtcaagctg gaccacgaac ccggtctgcg catccccaat atgttcgaag
     5881 cggcgctgga cggcagctac aagggcatct acgtcgaggg cgaagacatc gcccagtccg
     5941 accccaacac gcagcacgtc accgcggcac tggaagccat ggagtgcgtg gtggtgcaag
     6001 acctgttcct caacgagact gccaagttcg cccatgtatt cttccccggc gcttcgttcc
     6061 tggaaaagga cggcaccttt accaacgccg agcggcgcat ttcgccggta cgcacggtga
     6121 tgccgccgct ggccggaaag gctgactggg aagtgacgca gatgctgtcc aatgcgctgg
     6181 gctaccccat gaattacaac cacccgtccg agatcatgga cgaaatcgcg cgcctgacac
     6241 cgactttcca cggcgtcagt tatgccagga ttgatgaact gggcagcatc cagtggccat
     6301 gcaacgaaca ggcgccggac ggtacgccca tcatgcacgt cgagcagttc gtacgcggca
     6361 agggcaagtt catgttcacc gaatacgtgc cgacggatga aaaggtgacg cataaattcc
     6421 cgctcatcct gacaacaggc cgcattctgg cgcattacaa cgtcggtgcg cagacccgcc
     6481 gcaccgccaa caccgcctgg cacccggaag acattctgga aatccacccc cacgacgccg
     6541 aggagcgcgg catcgccgat ggcgactggg tgggcatcca gagccgcgcc ggggagaccg
     6601 tgctgcacgc cagaatttcc atccgcatgc agccgggcgt ggtctacacc accttccacc
     6661 atccgttctc cggcgccaac gtcatcacca ccgacaattc cgactgggcc accaactgcc
     6721 cggaatataa ggtgaccgct gtgcaggtgg tgaaggtgac acaaccttcc gaatggcaga
     6781 agcgctaccg tcagttcagc gaacaccagc aggaactgtt gcaacagcgc gcagtggtaa
     6841 agacgcagta aatgagtacg gcaccgctgt cctgcgagcc gccgatggaa gccgagttcg
     6901 ccagccatac ccgccgcacc gtgaagcgct ggcgaggcgg ggcttgggta cacgccatcg
     6961 accaggtggc cgaggaagta ccggtggcca tggtctacaa cggcatttcg catgcggtca
     7021 tgctggccac ccctgtcgga ctggaagcct tcggcttggg tttcagcctg tccgaaggca
     7081 tcctgcacgg ccgcggtgag ctctacgatc tggaagtggt gcggcgcagg gaaggtatcg
     7141 agctgcaaat gaccatcgct accgaacgtt ttgccgaact caagcaacgg cggcgcaacc
     7201 tggctggccg aaccggctgc ggcctgtgcg gcactgaaag cctgcaacag gtgttccgcc
     7261 acccccagcc agtgccgggc ggcgatctgc acatgacgcc acaagccatc catgccgtct
     7321 tccgcgaact ggtgccgaca cagacactac aatcactcac cggcgccgct cacgcggccg
     7381 cctgggtatc acggcagggt gaattgctgc acgtggagga agacatcggg aggcacaacg
     7441 cactagacaa actgatcggc gccctggcgg caaagagcct gcctttcgat cagggctgcg
     7501 ccatcgtcac cagtcgcgcc agctacgaaa tggtacagaa ggcggccacc gtcggcatac
     7561 ccttgctggc cgccatctcc gccccgaccg ggttggccat ccaactcgcc gaacactgtg
     7621 gcgtcaccct gctgggcttc gtccgtcgtg acagccacgt ggtctacgcc cacccgcaac
     7681 gactcaaata acctgggaaa tctgcatgga catccatcac ctcgtcaaaa tggccaatca
     7741 gatcggccag ttcttcgaat ccgaacccga ccagcagcaa gcggtgcagg atatcgccac
     7801 ccacatcaag cgcttctggg atccgcgcat gcgcaagacc ctcatcggcc acatcgaaaa
     7861 cggcggcgca gggttgctgc ccattgtgat gaagtccctt caggaaaacc aggaatcgct
     7921 ggtctagttc gtatttcctg catggcgctt tacgcaaccc gtgtatgtga tgctccactt
     7981 atggcatcat gccgtaactt taaaaacagg ctcacccttt gaaaatcgtt ttagtctggc
     8041 gctggccagt gcggctgatg cactggggca tggtggcggc attcagcgta gccatgtggt
     8101 accgcgattc catcatggac cagttgtttc acgtctacgc cggctacgcc atggcggcca
     8161 tcctgttcgc ccgtattatt tatggttttg cggcgcgcga cttggcggca tttcgccgct
     8221 tccctcctgc gccaatcaaa ggggcaaaat acgctttcga cctgattcgc ggcaaagcca
     8281 ggaactatct gggccacaat ccggccgggg cgctggcgat ctacggcatg ctgctgctgg
     8341 gtctggcaac ggtcggcacc ggctactggg cattcgagta tgacaacgaa ctggccaaga
     8401 cctggcacca ccattttgcc tacacctggt tctggctgat ctgcctgcac ctgttcggcg
     8461 tgctgatggg cagcctggcg cacaaggagt tcctggtatg ggccatgatc accggttgca
     8521 agacccggcg cagcatcagc cagccgttct cactgcaagc agtgggtatc accttgctga
     8581 tcattttcct gcacctgctc aacctgattg tcatcctttt tggcgggaag ggcttcatca
     8641 accggaagta aggcctgacc cgttggtgcg ctgcgatcag ggacaccagc cctctaagcc
     8701 gtcacccgcc cctgctccaa tcgcatcttc accacatctt ccagcttcat caggtcgtga
     8761 tcgtgggtgg cgatcaggac gcagttgttg gcgtcgcgca tgtgacgaat cagttcagcg
     8821 acctgctcgc gcgcggcgtc gtccaggttg gcggtaggct cgtccaacag caacagctcc
     8881 ggcttcagca cccgcgcgcg cgccagtgcc acgcgctgct tttcgccgcc ggaaagacgg
     8941 tacggtggca cttcggccaa gtgttcgagg ttggcccagg ccatttcaac ccgcacccgc
     9001 gcttcgcgct cgctttttgg caggccgctg aggccgtagc cgatgttgtc cgccacgctg
     9061 gtgttgaaca cgtaggagtg ctggtgcacg tagatcatgc gcggcgccag ccgagcgcgc
     9121 gccggagcct cgtcataacg cacgccatca aacgtgaaat ttccggcctg cgcccgttcc
     9181 aggcctgcca ggatgcgcag cagagtggtc ttgccgacgc cgttgtcgcc catcagcaaa
     9241 tagcttgtgc cgcgttgcaa ctccaagcat ttaatgtcga gcagggtacg gtcaccgtag
     9301 gccttgcgca gtccctctat ggaaagtaac ggggtcactt gatctcccct ttgccctgca
     9361 ggaaggcaat accaaaattc acacccagcg ccagcgccac cagcaccacg cccagcgcga
     9421 tgccttgggc gaactcgccc ttgctggttt ccagggcaat ggcggtggtg atgttgcgcg
     9481 tcacgccggc gatgttgccg ccgaccatca tggcgcagcc gatttccgac agcacgcggc
     9541 cgaaaccggt agcaatcgcc gccatcaccg cgaaccgcgc ttccagcaat acggtgaacg
     9601 aggcgcgtag cggcgaggct cccaaagtac gcgccgtttc cgacacgcgg gcatcgatgc
     9661 cctgcaccgc gctcagggtc atggcggtga ggatgggaaa ggccagcagc gcctggccga
     9721 tgaccatacc ctgctgggtg aacagcagtt cgaagctgcc cattggcccg ttgcgcgtca
     9781 gcagcaggta gacgatcagc cccacgacca ccgtaggcag ggcgagcagg gtctgcatca
     9841 gcacgatcaa ggcgcgtcgg ccgggaaatt gcagtgtggc cagcccgaac ccggccacga
     9901 tggccagcgg ggtaacggcc agcagcgcca gcaaggcaac gcgcagcgac acccagacga
     9961 tgccccagat agtgagatcg ccttccagca gccagcggaa cgcttgcagc gtcgcttcgg
    10021 ttagccccat gtacattatt tcgcgtcagg gatgaacaac tgctcccccc ccaccttgaa
    10081 gtcggcgatc agcttctgcc cttcggccga ggtgatccag tcggtcagcg ccttcgcgcc
    10141 ctgatagttg gcactgggat atttcttagg attgactgcg atgatgccat aggggttgaa
    10201 catacgggga tcgccctgta ccagcatggg cagaccgatc ttttcacggt aggcgatcca
    10261 ggtgccacga tcggtcaggg tgtaggcgcg catttcgccc gacatcatca gcacctcgcc
    10321 catgccctgt ccggcagaaa aataccaggg cagcccggcc gggttgatat cgagcgcctt
    10381 ccagtaactt ttttccatct ggtcggtgcc ggagtcatcg ccgcgcgaga caaacttcca
    10441 tttggcatcg gcaatctttt tcatcgccgc gagcacatct ttctctcccc ggattcttgc
    10501 cggatcgttg tttggcccga cgagaacaaa atcgttgtac atcacgtccc ggcgatcaac
    10561 accgtagcca gcggcgacaa aggcatcttc cgccgggcgg gcatgcacca gcaccacgtc
    10621 cacattgccg tcctcaccca gcttgagcgc cttaccggtg ccgacggcga tgacgttgac
    10681 cttgcaaccg catttgacct cgaatttagg cagcaggtaa tcgagcaagc cggtgttggc
    10741 agtgctggtc gtggtagcca ggcggatcgc cttagaggag ccggcacctg caggaaagga
    10801 aatgagggcg gagaacacca ctaccgccca tagttgcatc gtacgcttga tcatgtgggt
    10861 tggtctcgct tagaagtcaa attgtgtacg caggacaaga tcattttcaa aatcctgggt
    10921 cctgccgttg aatgtaacgg ggtcatcaaa gctggtgtgg atatagttga gcatgatgcg
    10981 ggcattcgga ttcaatatcc acttcacgcc cgtagtccac gcatccgcct ggctggtata
    11041 gcaggtgttg gtgctgaccg cagccgggtt actggaaccg catccctttg tacccgtagt
    11101 gttcgctaac atgctgaccg aactgcccga gggcttattc gcaaaatcgg aagcgtcgaa
    11161 cttgctgtag cgcaggccga attcgatcgc gccccagcca tccttgccaa aagtcaggtt
    11221 gtttttcggg ctcatacggc cgaatacacc gctcttgtag ttatccgcat agctctcacc
    11281 agtcaacatc cagttcaggt ccacgtacca gttgttaatg tcattgtcgt agtgataggt
    11341 gctgctgccg gaattccatt tgccatcgaa attggcgtgg atgtattcgg attgcagctt
    11401 gaccgggcca taggtcagcg cggtttcaaa ccccaggcgt gtccggtcgc tattgccgtg
    11461 catgccgttg gtcgtactga agaacttcac ccccatgcct tcggtttgct gggagatcag
    11521 ccccgtgttg ctacagggat tgggcccgct gctgcaactg gtatcgaagc cgaggttgcc
    11581 ggtactcgcg gaagtcccga catgcagcac cgcattcttg atgtcggcca actgggcaaa
    11641 gttggcattg atacgcgcac cggtgtcctt ctggttattg aagtcgttgg cgttcatgcc
    11701 agtcccgttg accgcgtaga tattgtaatt caccccagga accgggtcgc cgaacaccat
    11761 tacgccacgg tcataagtgg aattgaagat ggtaccgttg ttggtggcca aagagagttc
    11821 cgtgaagtcg gtgaaattgg tactctcagc gcgctccagg ccaaagaatg gcttgaactg
    11881 gcccaaccgg atgttggcct ctttccagtg gccgtaattc aggtaggcgt aagtcaggcc
    11941 ggtggtggct gaactattgt tgttgtacga agtagcggag gcgctcggca cattggcgta
    12001 ttcgccttcg acgcgaattg agtaatcctt gaggaaggtg aaagtacccc caaaacgtgc
    12061 gcggcgtatg ctgaaggtat cgctttgcca ttcactggga ttgtagtcgc ggtaatccgc
    12121 ctgcacgcgg ccgttcagct gcagtttcca gttgccactg ccgtcgtcga ataccagacc
    12181 atccttgaat gcggcataca ccgggctgcc agtgctcttg cccttttcct tgaccttctc
    12241 ctccttgccg ttcgccatct gctgctgcat ggccttgagc tcctcggcca tcacctgcaa
    12301 ttgcttgttc tgctcttcac gctgcacctg catttgctgc attatttttt ccaactgctg
    12361 gatgcggacc gccggatcca gcagctcctc agcgtaagcg ccgcttgaaa cggcagcaag
    12421 caccagtgcg gccagcttgc gtttatttcc cccaattgct tgatgcatga ttcctccctt
    12481 cgagtgttgc tgaaagacgc cagcatattg aaatattttt tggaatatat cgcacaatgt
    12541 attttattgg atacaacgga aaggcgacaa cattgctgga aagaatgtaa atcgataggt
    12601 gaagggcgaa acaggttacc gcttattcgg tgccagcatt cccaccagtt cctgcagttc
    12661 ggccgccacg ctttgctcgg ccttagcaac caaggcacgg tagcggtgcc gtacttcgtg
    12721 gccaaactcg gtcacctgtg caccgccgcc atgactgccg ccggtagccg tggataccag
    12781 cggctggcga aagcagcggt tcatggtgtc caccaagtcc caggcgcgct tgtaggacat
    12841 gcccatgttg cgcgccgccg ccgaaatcga accgctgctt tcgatggcat caagcaggtc
    12901 ggccttgccg ggacccattg cggacaaagt gccgtagggg atacgaatct taatgttgct
    12961 ggaactcata gtcttctatg gatatctgat taaaatagtc ggataatttt gttttgtggc
    13021 atcagtatga aacgcttaga attctgcacc ctcgggataa tttacgccat agtctgacac
    13081 catccattgc catgggcttc atcgacagca attctgccgt attgatcgac cgcttcggac
    13141 ggcgggtgga ctatatccgc ctgtcgatca ccgaccgttg cgatttccgt tgcgtttact
    13201 gcatgggcga ggacatgacc ttcctgccac gtgagcaagt gctcaccctg gaggagtgcg
    13261 agcggctggt gcgggtgttc gtcgagctgg gcgtgaggaa ggtgcgtatc accggtggcg
    13321 agcctctggt ccggcgcgat gcgctgtggc tgttccagca tatcggcaga ctgtccggcc
    13381 tggacgaact aactctcacc accaacggtt cgcaactgga gcaatacgcc gggcagctca
    13441 aggatgccgg cgtcgaccgc atcaacatca gcctcgatag cctggacgcc gagcgcttcc
    13501 gcaaggtcac ccgcatcggc aacatccaca aggtgctgcg cggcatcgaa gcggcccgag
    13561 ctgccggctt tgcccacacc aagctcaaca cggtactgat gcacggcttc aacgatgacg
    13621 aggcgccaca gctggtgcaa ttcgccatcg acaagcagct ggacatcgcc ttcatcgagg
    13681 aaatgccgct gggcgaggtg gatcacctgc gcgcgtcgag ctacgtcagc aatgaagaaa
    13741 ccctgaaaac cctgcaaggc catttcagcc tggtcagcag cgccgaaacc accggcggcc
    13801 cggcgcgcta ctggcgcgtt ccgggcacca gcacccgcat cggcttcatc tcgccgcaca
    13861 gccacaactt ctgtgaatcc tgcaaccgtg tgcgcatcac ctgcaagggc gagctctatc
    13921 tgtgcttggg gcaggaggat aaggtcgatc tcatgccgta tctgcgcgac caccccgacg
    13981 acgatgcgcc gctacggaac gccatcctga actccatgga gatcaagccc aaggggcatg
    14041 acttcgacct gcgccgcgcc gaaccggcag tggtacgctt catgtccatg accggaggtt
    14101 gatggtgtcc agactccgcc cacaaatcac ccatgccagc cgcccgctca ctttcaccgt
    14161 tccatccatc gacgagcatg gtgaagccgg caccacggaa atcgccggcg aaggcccgct
    14221 gacgctgtat ctggacaagc gcgagatcgt caccctgatg accctgggcg ccgcgccaga
    14281 agcgttggcg atcggctatt tgcgcaacca gaggctgctg cccgacatcg aggaaattgc
    14341 cgcagtgcaa gtggactggg aaaccgacgc cgtggccgtc accacccgca acggtatcgc
    14401 caacatcgag cagcgccttg gcaaacgcac tgtcaccacc ggctgcggcc agggcaccat
    14461 gttcggcgac ctgatggaag agctcgacca actccggctg ccacacgacg ccaccttgga
    14521 cgaagccacc ctgcaagcct tgcttgacaa cgtgcgccat tacgacagcc tgtacaagaa
    14581 agccggcgcc ctgcacggct gcgcgctggg ccagggggca gaaattctct actacgtcga
    14641 ggacgtcggc cgccacaacg cagtagatgc catcgccggc atgatgtggc tggacgacgt
    14701 cagcggccat gacaagactt tctacaccac cgggcggctg accagcgaaa tggtcatcaa
    14761 ggcggcacag atgggcatcc cgtttctggt gtcgcgctcc ggcctcaccc gcatgggctg
    14821 ggaaatcgca caaaaggtcg gcatcaccat gatcggccgc gcccagggtc ggcatttcct
    14881 gctgttcacc ggcgccgagc gcttccgcca caccgagaaa gcacaggcgg cggcatgaac
    14941 gtcaccggca tcgttctcgc tggcggcatg gggcggcgca tgggcggcgt ggacaagggg
    15001 ctgctgccgt tccacggcaa gccgctggtg gcgcacgtac tggaacgcct gcaaccgcag
    15061 gtgacggaaa tcctgctcaa tgccaaccgt gaaatcgagg cctaccaggc ttttggctac
    15121 ccggtcatca gcgacgccat cggcggtttc gccgggccgc tggccgggct gcacagcggc
    15181 atgtcagtgg caaaaacgcc attggtcgtc accgtgccct gcgattcgcc tttcctgcct
    15241 gccgacctgg cgcagcgcct gttgcaggcc atggaaaaac atcaggccga cctcgccgta
    15301 gccaaaaccg gcgaccagcc acacccggtg ttctgcctgt gccgcacctc gctgctgccc
    15361 ggcctgaccc gtttcctgga gagcggcggg cgcaagatcg atgcctggta tgccggcctc
    15421 aacgccgtag aagtgccgtt cgacgatgag gcggatgcct tcgccaactt caacacaccg
    15481 gcggaactgg aggccgcggc atgatccgct gcgaagtgcc attgctcggc ttttgcgcct
    15541 ggggcagcgg cgtcggcaag accaccctgc ttaccagtct gatcccggta ctcacgctgc
    15601 atggcctgcg tatttccgtc gtcaagcacg cccaccacac tttcgacatc gaccatccgg
    15661 gcaaggatag ctaccggctg cgcgaatccg gcgccatcca gacgctgctc ggctcgcgcc
    15721 agcgctgggc attgatgacc gagctttcgc acatcgaacc ccagcgacag gagcccagtc
    15781 tggtcgaact gctgacccac atggacacca cactggcaga cctgatcctg gtggaaggtt
    15841 tcaagcagga ggctatcccc aagatcgagg tgtaccggcc ctcgctcggt acgccgctgc
    15901 tggcagacca ggatccgcac gttatcgccg ttgctgcgga cggctcggcg ccgaccaaac
    15961 tgccactgct cgacctcaac gacccggttg ctatcggcga attcgtggtg gcatggctaa
    16021 agcaacagcg caccacgctg aaaattgttt ccacccatta agccatcacg atcatgacca
    16081 ccgatttcat caaggccgcc agttgcgccg acgattacga cccgaattcc atgccggtgg
    16141 acaaggcgcg caaatacatc cgcgactacc tcactccagt gcaaagcaag gagtgtctgc
    16201 cgctgcgcag cgcacttggc cgcgtgctgg cggaggacat cctgtcgcca tgcaatgtgc
    16261 ccaaccacaa caattcggcc atggacggct acgcgctcaa cgccgacgac ctggcagcca
    16321 cgggcgccac cacgctgagg atcgccggta ccgctttcgc cggcgagtcc ttcgccggca
    16381 agatgggtaa gggcgaatgc gtgcgcatca tgaccggcgc cgtcatgccg gagggctgcg
    16441 acagcgtggt gattcaggag catgtgcaga cggagggcga ccgcatcagc ttcggcgaag
    16501 gtgtcaaacg cggtcagaac ctgcgctacg ccggcgaaga cctcaagctt ggccagaccg
    16561 tgttctccgc cggacatctg gttcgcccgg ccgacctggg gctgattgct tcgctcggta
    16621 tcggcgaagt acgggtgtac cggaaactgc gcgtcgcctt cttttccacc ggcgacgagc
    16681 tcgccagtat cggccagccc ctgcaggagg gccaggtcta cgacagcaac cgctacaccc
    16741 tgtacggcat gctggcgcgt ctgggagtgg agatcatcga catgggcgtg gtgcgcgacg
    16801 accccgggtt gctggagcag gcattgctcg acgcatccgc cgcggccgac gtcatcctca
    16861 ccagcggcgg cgtttcggtg ggcgaggccg acttcatgaa gcaactgctc aacgacctcg
    16921 gccaggtcgt gttctggaaa atcgcgatga agccggggcg gccgctggcc tacggcaaga
    16981 tcgccaacgc ccactacttc ggcctgcccg gcaacccggt gtcggtgatg gtcacgttct
    17041 accagttcgt gcgcgacgcc ctgctggtgc tgatgggcca acccaacccg gcgcccatac
    17101 cgttgctgaa cgccacctgc accgaagccc tgaaaaaagc cccgggccgc accgagttcc
    17161 agcgcggcag cctgtttctc gatgctgaca acagctggaa agtccgcccc atcggcaacc
    17221 agggttcggg cgtgctgcgc tcgatgtccg aggccaactg ctttatcgtg ctggcggatg
    17281 attgcggcaa tgttgcggcg ggtgcaacgg tgcaggcgca actgttggaa gggctggtct
    17341 agtaaaccac gctgctgatg gctggccgca gtctacaaat tcatgaaaat catgatgggg
    17401 taatgcgtca acccgcgcta cccacacgcc cccacaacct cgacaatatc cttatatcta
    17461 taatggcttc cctggagtgc tcaatgtcat cgcctagct
//
LOCUS       DAOMFA010000013        16601 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_259_length_16601_cov_5.459507, whole genome shotgun sequence.
ACCESSION   DAOMFA010000013 DAOMFA010000000
VERSION     DAOMFA010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 16601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16601
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_259_length_16601_cov_5.459507"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            42..118
                     /locus_tag="PKW44_01385"
     tRNA            42..118
                     /locus_tag="PKW44_01385"
                     /product="tRNA-OTHER"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
     gene            complement(275..700)
                     /locus_tag="PKW44_01390"
     CDS             complement(275..700)
                     /locus_tag="PKW44_01390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR50273.1"
                     /translation="MNKLLASLIAGLFGFSVNAAADGKSAFGVAGQSDRHTSGESQET
                     THGPFAVDRNEEQWRAEDRRSAEGQDRHKSNEAHERYGRYGREGREGREGREGHDRQE
                     RREGYDGHDQQDRHEGHDRHEERNGRDGYNYQGNRVKNH"
     gene            877..1206
                     /locus_tag="PKW44_01395"
     CDS             877..1206
                     /locus_tag="PKW44_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019898976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR50274.1"
                     /translation="MEIRAGEIYGKRNASRLVMYSVLKVTRKSIILQNIDNPLSLFET
                     TAEKLERSGYERISQTPYVDTHCRGKKRKAAARRPSRCPYTLDFLEGRADCEKPVQAD
                     SVHAGPA"
     gene            complement(1188..2504)
                     /gene="rimO"
                     /locus_tag="PKW44_01400"
     CDS             complement(1188..2504)
                     /gene="rimO"
                     /locus_tag="PKW44_01400"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HQR50275.1"
                     /translation="MAKQLPQVGFVSLGCPKASSDAERILTQLRAEGYEISGSYQDAD
                     LVVVNTCGFIDSAVEESLDAIGEALAENGKVIVTGCLGAKGSVVRDAHPSVLAVTGPH
                     ALDEVMEAVHAHLPRPHDPYTDLVPPQGIRLTPRHYAYLKISEGCNHRCTFCIIPSLR
                     GDLVSRPVGEVMHEAETLVKSGVKELLVISQDTSAYGVDVKYRTGFWDGRPIRTRMTE
                     LARALGELDVWVRLHYVYPYPHVDEVLPLMADGLVLPYLDVPFQHASPHILKAMKRPA
                     SSENNLNRIRAWREICPELTLRSTFITGFPGETESDFQQLLDFLQEAQLDRVGAFTYS
                     PVEGASANALPDQVPEEVKQERLARFMEVQARISADKLQQKIGQTMLVLVDEADDENA
                     VGRSMADAPEIDGIVYLEGGAGLLPGDLVEVEITGADEHDLYAGPA"
     gene            2519..2740
                     /locus_tag="PKW44_01405"
     CDS             2519..2740
                     /locus_tag="PKW44_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR50276.1"
                     /translation="MYIVAIAWLYVTLMMALTEPSIVAGVLTFVCYGLLPCALLLWLA
                     GTPQRRRNQLRKVTEQQPGQPDGADAEGD"
     gene            complement(2678..3469)
                     /locus_tag="PKW44_01410"
     CDS             complement(2678..3469)
                     /locus_tag="PKW44_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HQR50277.1"
                     /translation="MTTLTSILVASFLGGILSVCAAGLVAFKARVHWIPVLVSYAIGA
                     LLGAAFLEIIPEAFETAKSAQSMAATLLFGILLFFVLEKLVLWRHCHVEECEAHAPDH
                     DHGHHHAHDHGRSGLMITVGDTFHNFVDGILIAAAFLADFNLGVVTALAIIAHEIPQE
                     VGDFLILLHSGYSKRQALLLNLLSSVATLVGGVLAYYALGTMQNVVPYVLGLAASSMI
                     YVAVADLIPGLHKRPQLRDTAQQVTLIALGVGSIWLTRLLLGDLA"
     gene            complement(3493..3978)
                     /locus_tag="PKW44_01415"
     CDS             complement(3493..3978)
                     /locus_tag="PKW44_01415"
                     /inference="COORDINATES: protein motif:HMM:TIGR02241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HQR50278.1"
                     /translation="MAIARGNPYGAFNFQVSLDARVIGGFSDVSGIGAATSYSEYREG
                     TIPRDTSRKTAATQKYADVVLKRGLVDSASLSQWLEQGLKSGDSRRNLTITLLDEAGQ
                     PVAKFHLCNAQPQKWVGPTLAAKGGDVAMEELHLTHEGLSCEETDSHPRQTPGRPPRK
                     P"
     gene            complement(3998..5872)
                     /locus_tag="PKW44_01420"
     CDS             complement(3998..5872)
                     /locus_tag="PKW44_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HQR50279.1"
                     /translation="MPEYLAPGVYVEEVSFRAKSVEGVPSSTAGFAGLTRYGPVHYRD
                     GPSTSQPRLITSFAEFERVYGGLDPLQINGHARVNYLAHAARAFFINGGKRLYVSRVF
                     LDSNADPASTHVASLNVGAATWRARWPGKSGNVQVETRVRRSDNLAFRHNLDPGDKLA
                     RHLWGVQVQGATNGSVVELTHPLPKPPETLPGNNDPLQAENLYVVKTDSNGRQTFVDR
                     HGAEGQLPDDLTDMQVLLVELEVVVTGPGSRADAYQRLCGHKNQKRYIGKVLDRDKPE
                     EENAIVYLDYTYDESDPFTAARLMVNLHGATQKQLAGGDDGKLPGPGELEGKTASPGR
                     SGAGASGLEALGPVDEVAIVALPDAATLPDLDLVVDATRRLIAHAEKYRYRTAVVDSP
                     PQCSISEVRTFRSKFDSSYAAMYYPWIEILDPLAPAVSGTSPDSLLLPPSGFVCGIYS
                     RSDIERGVHKAPANEIVRGLTRFEVDINKAHQEVLNSEGVNCLRFFEGRGNRVWGART
                     MSSDPEWKYVNVRRLFIHLEHSLDKATQWVVFEPNNERLWANVRRMMEDFLLARWQDG
                     ALLGQKPEQAFFVRCDRSTMTQNDLNNGRMVCLIGVAPTRPAEFVIFRIGQWTADTKT
                     "
     gene            complement(6004..6738)
                     /gene="pgeF"
                     /locus_tag="PKW44_01425"
     CDS             complement(6004..6738)
                     /gene="pgeF"
                     /locus_tag="PKW44_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HQR50280.1"
                     /translation="MRLRQEHLVVPDWPVPPGVQALQTTRLGGVSLPPYDSLNLGMHV
                     GDDPLAVSANRHALSPMLPSEPVWLEQVHGTTVVLAESAGRHPLADACVTRKKNTVCV
                     VMTADCLPVLLCDRAGTVAAAVHAGWRGLAGGVIEATVAAMAMPEEQLMAWLGPAIGP
                     QAFEVGGEVREVFLAHDPAAATAFTPHAGKYLVDIYRLARQRLHAMGIMQIHGGGFCT
                     HGDRERFFSYRRDGRTGRMGSFIWLS"
     gene            complement(6735..7772)
                     /gene="rluD"
                     /locus_tag="PKW44_01430"
                     /pseudo
     CDS             complement(6735..7772)
                     /gene="rluD"
                     /locus_tag="PKW44_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882140.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
     gene            7816..8616
                     /locus_tag="PKW44_01435"
     CDS             7816..8616
                     /locus_tag="PKW44_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HQR50281.1"
                     /translation="MKRSLALFLALLLSGCFIFGNPTEVDETKGWTVQKLYNEATISL
                     QDHDYEKALKYFGILESRYPHGRYAMQAQMETIYIYYKKGDPTAAIAAADRFIKLHPH
                     HPNVDYAYYLKGLAVFNERGIVEKLTGQEISDRDPKALRESFAAFKELVTLYPNSKYV
                     KDATARMTYLVNTLAEHELHVARFYMKRKAYIAAVNRCQYVLTNYPQAPAVEEALVIM
                     ISAYDLMGMDDLKQDAQRVLKANYPDSRFNTTTVPSDEGTWWKFWEAL"
     gene            complement(8678..10105)
                     /gene="der"
                     /locus_tag="PKW44_01440"
     CDS             complement(8678..10105)
                     /gene="der"
                     /locus_tag="PKW44_01440"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HQR50282.1"
                     /translation="MLPTIVLVGRPNVGKSTLFNRLTKTRDALVADLPGLTRDRHYGR
                     GAVGDFPYLVVDTGGFEPLADSGILVEMARQTLLAIDEADVIVFLVDGRQGLAPQDKV
                     IADRLRRCERPVFLAVNKTEGMNRAVASAEFHELGLGDPLSISSAHGEGVRDLVNLVL
                     EDFLPEQTETAESTSSKAPKVAIVGRPNVGKSTLVNALIGEQRVIAFDQPGTTRDSIY
                     IDLERNGRHYTLIDTAGVRKRGKVFEAIEKFSVIKTMQAIEDANVVILVVDAMDGITE
                     QEAHIAAFILESGRALVVAVNKWDGLDRDKREWIKHEIDRKLQFLDFAEFHYISALKG
                     SGLTPLLQSVDVAYKSAMAKLSTPRLTRVLEEAIQQHQPPISKGIRPKLRYAHQGGSN
                     PPIIVVHGNHVDGVKQSYVRFLEGVFRKAFELSGTPLRVQFKQGANPFAEAEDKRKPG
                     EGIVSMRRRKTAQRAELKARKEKKK"
     gene            complement(10168..11448)
                     /gene="bamB"
                     /locus_tag="PKW44_01445"
     CDS             complement(10168..11448)
                     /gene="bamB"
                     /locus_tag="PKW44_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HQR50283.1"
                     /translation="MLQRPVLFLAAALLTACTSLTDLKTSVSESIFGPEPKDPPMELG
                     EFKPTATTKVLWHSNVGKSKFYEFSPALDGAAVFAAGGEGEIVRLEATSGKQVWRINA
                     GEQLSGGVGAGDNLVLVGTPKGMVLAFDQNGKPLWKSKVSSEVLSAPKISSGVVVVRT
                     GDSRIFGLSAADGKRKWVYERSTPALSLRSAAGVALDGGAAYAGFAGGKLIALKVDDG
                     KVLWEASVAQAKGTTEIERIADITSQPVVDGRMVYAVAFQGRVAGVDRTNGRVLWTRD
                     ISSYTGLGAEDARVYVSHAGGALYALDYNSGKSYWRQGSLLNRLLSAPMPIGDYVAVG
                     DVEGYVHFLASEDGAFAARIRTEDSPVMAHLVDMGNSSLLAQTRDGGVYAIAIQPMAR
                     KDSPTPDSSGVAPREPASPEKKKADGLNSLDLHD"
     gene            complement(11448..12074)
                     /locus_tag="PKW44_01450"
     CDS             complement(11448..12074)
                     /locus_tag="PKW44_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQR50284.1"
                     /translation="MAYDLEEQEQLDELKAWWKRHGNTVMLGLGLALAAFAAYQGWKA
                     YQHRQSLQASAQYDALTQLEEKDIKGIRSISGQIIEKYAGTPYAVRAALIAAKANYAA
                     NDAKSAKAQLEWAMGHAKEDALRAVAQLQLAAIQFDEKQYDAALKTLGEKHDAGFDGL
                     FADLKGDILAAQGKKAEAKAAYAEALTKLDAGGDYRHHTEHKLDALGS"
     gene            complement(12084..12896)
                     /locus_tag="PKW44_01455"
     CDS             complement(12084..12896)
                     /locus_tag="PKW44_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQR50285.1"
                     /translation="MSGSILSAKGLTKIYKQRSGRFGFGTSLVRALDNVSISVRQGAT
                     LAVVGESGSGKSTLARCLLQLQPLDAGEVQFEGRDLAALTGTQLRAARRHMQMVFQDP
                     FASLNPRMKVGEIVGEGLLIHKLGNAAERRDKVECMLQRVGLSVADMEKYPHQFSGGQ
                     RQRIGIARALVLEPKLVVCDEPVSALDVSIQAQILLLLKELQQEMGLSYLFISHDLRV
                     VRHVADDIVVMHRGRIVEQGKVERIYSDPQQPYTRNLLNSIPGRRHLAGLAT"
     gene            complement(12893..13726)
                     /locus_tag="PKW44_01460"
     CDS             complement(12893..13726)
                     /locus_tag="PKW44_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQR50286.1"
                     /translation="MNALLQIEGLTVAPATQPQRLLVSNVSLTLQAGNTTCIVGESGS
                     GKSLTALSVMGLLPPALQRRSGRVLFQGNDLAGYSNTRMQDVRGKRIGMIFQEPMTSL
                     NPVLTVGYQIGETLAFHLGLGGKALQARIAALLEQVGIPPERAGSYPDELSGGQRQRV
                     MIAMAIACEPAMLIADEPTTALDVTVQAQILSLLNELKQRMNMGMLFITHDFGVVADI
                     ADEVAVMFRGEIVEMGAVDKVLGNPEHPYTRALLGCVPDAEGKKPLKPIDYAWLNEAA
                     A"
     gene            complement(13726..14982)
                     /gene="hisS"
                     /locus_tag="PKW44_01465"
     CDS             complement(13726..14982)
                     /gene="hisS"
                     /locus_tag="PKW44_01465"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HQR50287.1"
                     /translation="MSKTIQAVRGMNDILPADAALWEFFEDTIRDWLRAYGYTQIRTP
                     IVESTDLFVRSIGEVTDIVEKEMYSFEDQLNGEHLTLRPEGTASCVRAVIQHNLLYNA
                     PQRLWYSGPMFRHERPQKGRYRQFHQVGVESLGYDGPDMDAEHIVMTARLWKLLGLQD
                     VALEINTLGSAEARARYRERLIAYFEQHTELLDEDAKRRLYSNPLRILDTKNPAMQPV
                     VEAAPSLADDLDDASLRHFEALQAMLKVAGVEYRINTRLVRGLDYYNATVFEWVTTRL
                     GAQGTVCAGGRYDSLVEQVGGKPAPACGYAMGVERLLALMQEQGFVPPANAPDAYVVH
                     AGEAASRWAPQVAEQLRNEGLAAVLHCGGGSFKSQMKKADASGARFAVIIGDDEAAAQ
                     MVSLKPLRQAGEQARVDLTQLVDLLD"
     gene            complement(15104..16504)
                     /locus_tag="PKW44_01470"
     CDS             complement(15104..16504)
                     /locus_tag="PKW44_01470"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018076776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HQR50288.1"
                     /translation="MQLTFLGAAGEVTGSCTLVETGATRFLVDCGMFQGGHEADLKNQ
                     RFPLFDPASIDFILLTHAHIDHSGMLPRLAARGFSGPIYATLATIELLDVMLRDSAHI
                     QAKEAEWEERHPHRNGHDEPSPPLYSLLDVEKALALLQPVEYASQFGPRAGVVCCFLD
                     AGHILGSAIIEVWLGEDGDRRKIVFSGDIGQPGRPILRSPTPVDSADILLVESTYGNR
                     LHKNLDDTLDEFVRAIERTLLEKHGNVIMPAFTVGRTQEILYLLIDLTRQRRLHNLKI
                     YVDSPLALAATEITMHHIELFNQEARKLLTWGEERRKELPDIRFTQTVEESMALNDIK
                     SGAIIISASGMCDAGRIKHHLRYNLNRPQSSIIFTGFQAEGTLGRRLVDGAKLARIFG
                     EEIVVRADIYTIGGLSAHADQAGLLEWLGYFKHTPRQTFVVHGESATAEQFAGLIRSR
                     LGWNARTPEMGEKTTI"
BASE COUNT         3026 a         4999 c         5022 g         3554 t
ORIGIN      
        1 gaaacttata agatgatgta tttaaacata aaataagcat tggcgcgggt tggaaaaggg
       61 gtgtaaattt gccttcacac ggcaggggtc actggttcga tcccagtacc gcccaccata
      121 aatcaatggc ttacggttaa tttccgtagg ccgttttggt ttgtgtgtaa tttttatctt
      181 caatcctagg ccaaattaaa aggggtttcc cctttccgtg cctacccata tcattcattt
      241 aatgaagcag ggccatagtc gtcatgatcc ctaattaatg gttttttaca cggttgcctt
      301 gatagttata cccgtcacgt ccgttacgtt cttcatgccg gtcatgtccc tcatgtctat
      361 cctgctggtc atgtccgtcg tatccttcac gtctctcttg cctatcatgc ccttcacggc
      421 cctcacgccc ctcacggccc tcacgcccgt aacgcccgta acgttcatgc gcctcgttag
      481 atttgtgacg atcctggcct tccgcgcttc gccgatcctc tgcgcgccat tgttcttcat
      541 tgcggtcaac agcaaaagga ccatgcgtgg tttcttggct ttcgccgctg gtatgtctat
      601 cagattggcc ggctactcca aaagcagatt ttccatcagc agcagcgttg acggaaaaac
      661 caaacagacc agcaatcaga ctggctaaca gcttgttcat gacatggcct ttcaggttgt
      721 ggatcaacag cccccgcagt taaacattac ggattacctt gaatagcata atacgcaaac
      781 cggtttcttt gaagcctaag atttgcgtgt gcttgctgac aggccgggaa accccatgta
      841 aactcgggcg caggaattct gactcggggg aagaggatgg aaatcagggc aggagagatt
      901 tacggcaagc gcaatgccag caggctggtg atgtacagcg tgctcaaggt aacccgcaaa
      961 tccatcattc tgcagaacat cgacaacccg ttgagcctgt tcgaaaccac cgcagaaaaa
     1021 ctcgagcgct ccggctacga gcgcatcagc cagacgccct atgtcgatac gcattgccgt
     1081 ggcaagaagc gcaaggcggc ggcgcgccgc cccagccgct gcccgtatac gctggacttc
     1141 ctggaggggc gcgccgattg tgaaaagccg gtacaggctg attcggttca cgccggcccg
     1201 gcgtaaagat cgtgctcgtc ggcgccggtg atttccactt ccactaagtc gccgggcaac
     1261 aaccccgccc cgccttccag gtagactata ccgtcgattt ccggcgcatc cgccatgctg
     1321 cggccgaccg cgttttcgtc gtccgcttcg tccaccaaca ccagcatggt ctggccgatt
     1381 ttctgctgta gcttgtcggc gctgatgcgc gcctgtactt ccatgaaacg cgccagccgc
     1441 tcctgcttca cttcttccgg cacctggtca ggcagcgcgt tggcggaggc gccttccacc
     1501 ggcgaatagg tgaaggcgcc gacacggtcg agttgcgctt cctgcaggaa atcaagcaac
     1561 tgttggaagt cggactcagt ctcgccgggg aagccggtga tgaaagtgct acgcagggtg
     1621 agttccgggc agatttcgcg ccaggcgcgg attctgttca ggttgttctc gctgctggca
     1681 ggccgcttca tggccttgag gatgtgcggg ctggcgtgct ggaacggcac gtcgaggtag
     1741 ggcagtacca agccgtcggc catcagcggg agcacttcgt ccacgtgcgg gtaagggtag
     1801 acgtaatgca gccgcaccca gacgtcgagt tctcccaagg cccgggccag ttcggtcatc
     1861 cgcgtcctga tcggtcgccc gtcccagaac ccggtgcggt atttgacgtc gacgccgtag
     1921 gcgctggtgt cctgcgaaat taccagcagt tccttcacgc cggacttcac cagcgtttcc
     1981 gcctcgtgca tgacctcgcc cactggccgg ctcaccaagt cgccgcgcag gctgggaatg
     2041 atacagaagg tgcagcggtg gttgcagcct tcggaaattt tcaggtaggc gtagtggcgc
     2101 ggcgtcaggc ggatgccctg tggcggcacc aggtcggtat aagggtcgtg cggcctgggg
     2161 aggtgggcat gcaccgcctc catgacttca tccagcgcat gcgggccggt gaccgccagc
     2221 acgctggggt gggcatccct gaccacgctg cccttggcgc ccagacagcc ggtgacgatg
     2281 accttgccgt tctccgccag cgcctcgccg atggcgtcca gcgattcttc cactgccgaa
     2341 tcgatgaagc cgcaggtgtt gacgacgacg aggtcggcat cctgatagct gccggaaatc
     2401 tcgtagcctt ccgcgcgcag ctgggtcagg atgcgctcgg catcggagga ggccttgggg
     2461 cagccgaggg aaacgaaacc gacttggggg agttgttttg ccatgttagt ctgaactcat
     2521 gtatatcgtt gcgattgctt ggctctatgt cacgctgatg atggcgctca ccgagccgtc
     2581 cattgttgcc ggtgttctca cttttgtatg ctacggcttg ctgccgtgtg ccttgctgct
     2641 gtggctggcg gggacgccgc agcgacgtcg caaccagcta cgcaaggtca ccgagcagca
     2701 gcctggtcag ccagatggag ccgacgccga gggcgattaa tgtcacctgc tgcgccgtgt
     2761 cacgcagttg cggccgcttg tgcaggccgg ggattaggtc ggccaccgct acgtagatca
     2821 tgctggaagc cgccagcccc agcacataag gcaccacgtt ctgcatggtt cctagcgcgt
     2881 aataggccag aaccccgccc accagggtcg ccacgctgga aagcaggttg agcagtaggg
     2941 cctggcgctt gctgtagccc gaatgcagca ggatgaggaa atcgcccacc tcctgtggaa
     3001 tctcgtgggc gatgatggcc agcgcggtca ccacgccaag gttgaaatcg gccagaaacg
     3061 cggcagcgat caggatgccg tcgacgaagt tgtggaaggt gtcgcccacc gtgatcatca
     3121 acccgctgcg tccgtggtca tgcgcatggt ggtgaccatg gtcatggtcg ggcgcatggg
     3181 cctcgcactc ctcgacgtgg caatggcgcc acagcaccag cttttccagt acgaagaaca
     3241 gcaggatgcc aaataaaagg gtggctgcca tgctttgcgc acttttcgcg gtttcgaaag
     3301 cttccgggat gatttccagg aacgccgcgc ccagcagggc gccgatggcg tagcttacca
     3361 gtacgggtat ccagtggacg cgtgccttga atgccacaag gccggctgcg caaacgctaa
     3421 gaatgccgcc cagaaagctg gcgacgagaa tggaagtcaa ggtggtcatg ggcattgatt
     3481 ataccggggt tgtcaaggct tccggggagg ccggcctggt gtctggcgag ggtggctgtc
     3541 ggtttcctca caagagaggc cttcatgtgt caggtgtagt tcttccatgg ccacgtcacc
     3601 gcccttggct gccagcgtcg ggccgaccca tttttgcggc tgcgcgttgc acaggtggaa
     3661 tttggctacc ggctgcccgg cctcatccag cagggtgatg gtgaggttgc ggcggctatc
     3721 gccgcttttc agcccttgtt ccagccattg actcagggag gccgaatcta ccaggccgcg
     3781 tttcaggacg acatctgcgt acttctgcgt ggcggcggtt ttgcgcgacg tgtcgcgagg
     3841 gatggtgcct tcgcgatatt cgctgtagct ggttgccgcc ccgatcccgc tgacgtctga
     3901 aaagccgccg atgacgcggg cgtccagaga tacctgaaaa ttgaaggcgc cgtatgggtt
     3961 gccgcgagct atcgccatga tcattcctcc atggttttta tgtcttggtg tcggcggtcc
     4021 actggccgat acggaaaatg acgaattcgg ccggacgcgt tggcgctacg cctatcaggc
     4081 agaccatgcg gccgttgttc aggtcgttct gcgtcatcgt gctgcggtcg cagcgcacga
     4141 agaaagcctg ttccggcttt tgtcccagta gcgcgccatc ctgccatcgg gccagcagaa
     4201 agtcttccat catgcggcgg acattggccc acagccgctc gttgttgggc tcgaacacca
     4261 cccactgcgt cgccttgtcc agggagtgct ccagatggat gaacaggcgc cgtacgttga
     4321 cgtatttcca ttccggatcc gaactcatgg tgcgtgcacc ccacacccga ttgccccgcc
     4381 cttcgaaaaa gcgcaggcag ttcactcctt cggaattcaa tacctcctgg tgcgccttgt
     4441 tgatgtcgac ctcgaatctg gtcaggccgc gcacgatctc gttggccggg gccttgtgca
     4501 cgccacgttc gatatcgctg cgggaataaa tgccgcagac aaagccggac ggcggcagca
     4561 gcagactgtc aggcgaggtg ccagacaccg ctggcgcgag cggatcgaga atttcgatcc
     4621 acgggtaata catggcagca tacgaactgt cgaatttgct gcggaacgtg cggacttcgc
     4681 tgattgagca ttgcggtggg ctatctacca ccgcggtgcg gtagcggtac ttttccgcgt
     4741 gggcgattag gcgccgggtg gcgtcaacga cgagatccag gtcgggcagg gtggcggcat
     4801 cgggaagcgc cacaatggcg acttcatcga ctggacccaa ggcttccagg ccgcttgcgc
     4861 cggctccgga ccgacccggg ctggcggttt ttccttcaag ctcgcctggc ccgggcagct
     4921 tgccatcgtc gccgccagcc aactgttttt gggttgctcc gtgcagatta accatgaggc
     4981 gggctgcggt gaatggatcc gactcgtcat acgtgtaatc cagatacacg atagcgttct
     5041 cctcctccgg cttgtcacga tccagcactt ttccgatata acgtttctga ttcttgtggc
     5101 cgcataagcg ctggtaggca tcagcccggc ttccggggcc agtcaccacc acctccagtt
     5161 ccaccagcag cacctgcatg tcggttaggt cgtctggcag ttgtccttcc gccccatggc
     5221 gatcgacaaa ggtctgccgg ccattactgt ccgtttttac cacatacagg ttctccgcct
     5281 gcagtggatc gttattcccg ggaagcgttt ccggcggctt cggcaatgga tgggtcagtt
     5341 ccactacgga accattggtg gcgccctgaa cctgtacccc ccagagatgc ctggccagct
     5401 tgtcgccggg atcgaggttg tgtctgaatg ccaggttatc gctgcgccgc actcgcgtct
     5461 cgacctgtac gttgccggac ttcccgggcc agcgtgcgcg ccaggtggct gcgccgacat
     5521 tcagcgaggc gacatgcgtg cttgccgggt cggcattgga gtccagaaac acacgcgata
     5581 catatagccg tttgccgccg ttgataaaaa atgctcgtgc ggcatgggcc agataattga
     5641 cccgtgcatg gccattgatc tgtaggggat caaggccgcc atagacgcgc tcaaattcgg
     5701 caaatgaagt gatcagacga ggttggcttg tgcttggccc atcccggtag tgcactggcc
     5761 cgtagcgggt caatccggca aacccggcgg tacttgacgg aacaccttcg acggacttgg
     5821 cgcgaaaact gacttcctca acgtacactc ccggggctag atactctggc atagcgtcct
     5881 ccctttttgg ccgggcgttt tccctactgc aacgagaaca gccgtattaa cgttgcagtc
     5941 ctgtccacaa cacaggctga gttatcaaag tagcatgttc atgcggctat tatatcggcg
     6001 ctttcaggac agccatatga agctgcccat gcggccggtc cggccatcgc ggcggtagga
     6061 aaaaaaacgc tcgcggtcac cgtgggtgca gaacccgccg ccgtggattt gcatgatgcc
     6121 catcgcgtgc aggcgctggc gcgccaagcg gtaaatgtcg accagatatt tacccgcatg
     6181 gggcgtaaag gcggtggccg ctgcaggatc atgggcaagg aacacctctc gcacttcgcc
     6241 gcccacctcg aatgcctgtg ggccgatggc gggacccagc caagccatga gttgttcctc
     6301 cggcattgcc atggcggcga cggtggcctc gatcacgccg ccggccagtc cgcgccagcc
     6361 ggcatgtacg gcagcggcga ccgttccggc ccggtcgcac agcagcaccg gcagacagtc
     6421 tgcggtcatc acgacgcata ccgtattctt tttgcgtgta acacaagcgt ctgctagtgg
     6481 atggcggccg gcactttcgg ccagcacaac ggtggtgccg tgcacctgtt ccagccagac
     6541 tggctcgctc ggtaacatgg ggctgagcgc atggcggttg gctgaaactg ccagcgggtc
     6601 gtcgccgaca tgcatgccga gatttaggct gtcgtaaggg ggcaggctga caccgcccaa
     6661 ccgcgtggtc tgcaaggctt ggacgccggg tggcaccggc cagtcgggga cgaccaggtg
     6721 ttcctgccgc agcctcattc ctcgtcctcg agatcttctt cgaaatcgtc ctcgaaatct
     6781 tcggcgtatt ccatagcgaa gtcttcgttg ccgacctctt cctcgaaacg catggcttcc
     6841 agcagcgctt tcatgtcgtc cggcagttcg gcctgccatt ccatggcttc gccggtggcg
     6901 ggatggatca gcccgaggcg caccgcatgt aaggcctgac gtttgaaatt cttcaaggct
     6961 gcgcgcaggg tgggggtgat ggccttgtgg ggcacgatgc cgcgcaggcc gtagaccggg
     7021 tcgcccacca gcggggcacc caggtgctgc atgtggacac ggatctggtg ggtgcggccg
     7081 gtttccagtc ggcagcgcag gtaggtgtgg acggcaaagc gctccagcac ctcatagtgg
     7141 gtgacggcag gcttgccgcc aacgcgcgcc accgccatcc tggtgcgggt gtgcgggtgg
     7201 cgcccgatct ggttttccac cgtaccgttc tgctacacct ctccccagac gatggcgcga
     7261 tactcccttt tgacggttct tgcctgcaac tgccgcacca ggctggtttg cgcggcgata
     7321 gttttggcta ccaccagcaa tccactggtg tccttgtcca accgatgcac gatgccggca
     7381 cgcggcaggt ccttcaactg cggtgcgtag tggagcaggg cgttgagcag ggtgccttgc
     7441 cagttgccgg cggcggggtg caccaccatg ccggccggct tgttgaccac caggatgtgc
     7501 tcgtcctcgt aaacgatggg caggtcgata tcttccggct tgaaggcatt ttcctccggc
     7561 ctgggctggg gcatcacttg cacgtgctcg ccaccccata tcttttgctt ggcgctggcc
     7621 ggctggccgt ccacgctgac caggccttcc ttgatccagc cctgcaggcg tgagcgggaa
     7681 tagtccggca gcagtttggg cagggcctgg tccaggcgca gcccgcccag cgattgcggg
     7741 atcaggacag agatgggttc ggattggggc atggttatag gttataattt tcggaaattt
     7801 cggtgggcca ttttcatgaa acgtagttta gcgttatttc ttgctctgct cctctccggc
     7861 tgcttcattt tcggcaatcc aacggaggtc gatgaaacca agggctggac ggtgcaaaag
     7921 ctttacaacg aagcaacgat cagcttgcaa gatcacgact acgagaaagc cctcaaatac
     7981 ttcggcattc tggagtcgcg ttatccgcat ggccgctatg ccatgcaggc gcagatggaa
     8041 accatctata tctattacaa gaaaggcgat ccgaccgccg ccatcgccgc cgccgaccgc
     8101 ttcatcaagt tgcatccgca ccatcccaac gtcgattatg cctattatct caaggggctg
     8161 gccgtcttca acgagcgcgg cattgtcgag aagctgactg gccaggagat cagcgaccgc
     8221 gatcccaagg cgttgcggga atccttcgcc gcgttcaagg agttagtcac gctctatccc
     8281 aacagcaaat atgtcaagga cgccacggcg cgcatgactt atctggtcaa caccctggcc
     8341 gaacacgaac tgcatgtggc gcgtttttac atgaagcgca aggcctacat cgccgccgtc
     8401 aatcgctgcc aatatgtgct caccaactac ccgcaggctc ccgcggtgga ggaggcgctg
     8461 gtcatcatga tcagcgctta tgacctgatg ggcatggacg atctcaagca ggatgcacag
     8521 cgcgtgctca aggccaacta cccggacagc cgcttcaata ccactacggt accgagtgat
     8581 gagggcacgt ggtggaaatt ctgggaggcc ctgtagaaac gggaggaaag tagggaggag
     8641 cagtaggaag taggttaatt tcccccgctc ctgcgcatta tttcttcttt tccttgcgcg
     8701 ccttcagttc ggcgcgctgt gcggttttgc ggcggcgcat gctgacgatc ccctcgcccg
     8761 gcttgcgctt gtcttccgcc tcggcaaacg gatttgcacc ctgtttgaac tgcacccgca
     8821 gtggcgtccc ggaaagctcg aacgccttgc gaaacacgcc ttccagaaaa cgcacgtagc
     8881 tttgtttgac gccgtccacg tggttgccat gcaccacgat aatcggcgga ttgctgccgc
     8941 cctgatgggc gtagcgcagt ttggggcgga tgcccttgga aatcggcggc tggtgctgct
     9001 ggatggcttc ttccagcacc cgcgtcagcc tgggggtgga gagcttggcc atagccgact
     9061 tgtaggcgac gtccaccgat tgcagcaagg gcgtcagccc gctgcctttg agtgccgaga
     9121 tgtagtggaa ttcggcgaag tccaggaact gcagcttgcg gtcgatctcg tgcttgatcc
     9181 actcgcgctt gtcccgatcc agtccgtccc acttattcac cgctactacc agtgcgcgtc
     9241 cgctttccag gatgaaggca gcaatatgcg cctcctgttc ggtgatgccg tccatggcgt
     9301 ccaccaccag aatcaccacg ttggcatcct cgatcgcctg catggtcttg atgacggaga
     9361 acttttcgat ggcctcgaat accttgccgc gcttgcgcac gccggcggta tcgatcaggg
     9421 tgtagtgcct gccgttgcgc tccaggtcaa tatagatgga gtcgcgcgtg gtgccgggct
     9481 ggtcgaaggc gatgacgcgc tgctcgccga ttagggcgtt gaccagcgtg gatttgccga
     9541 cgttggggcg gccgacgatg gcgaccttgg gcgctttgga tgaggtactc tcggcagttt
     9601 ccgtctgttc cggcagaaag tcttccagca ccagattgac caggtcgcgc acgccttcgc
     9661 catgtgcgga ggaaatcgac agggggtcac ccaagcccag ttcgtggaac tctgccgaag
     9721 ccacggcgcg gttcatgcct tcggtcttgt tgactgcaag gaacaccggg cgctcgcagc
     9781 ggcgcaggcg atccgcgatc accttgtcct gtggcgccag cccctggcgg ccatccacca
     9841 ggaatacgat gacatcggct tcgtcgatcg cgagcagggt ctggcgcgcc atctcgacca
     9901 gaatgccgct gtccgccagt ggctcgaagc cgccggtatc caccaccaga tagggaaaat
     9961 cgcctaccgc accgcggccg taatgacggt cgcgcgtcaa ccccggcagg tctgccacca
    10021 aggcgtcgcg cgtcttggtc agacggttga acagggtgga cttgccgaca ttggggcgtc
    10081 cgacgaggac gatggtgggg agcaaattat ctgccttcca tagcgcaggc ggcctcacct
    10141 gcagtttcgg attcccggtg agcgggctta atcgtgcaag tccagcgaat tcaggccgtc
    10201 cgcctttttc ttttcgggcg aagctggttc gcgtggggcc actccggacg aatccggtgt
    10261 gggtgaatcc ttacgtgcca ttggttgaat ggcaatagcg tagacaccac cgtcgcgggt
    10321 ttgcgccagc aggctggaat tgcccatgtc caccaaatgt gccatgaccg ggctgtcttc
    10381 ggtgcggatg cgggcagcaa atgccccatc ctcgcttgcc aggaaatgca cgtagccctc
    10441 cacatcgcca acggctacat agtcgccaat aggcatcggc gcactgagca agcggttgag
    10501 caggctgccc tggcgccagt agctcttgcc gctgttgtaa tccagcgcat aaagcgcacc
    10561 gccggcatgc gacacgtaaa cgcgcgcatc ctcggccccc aggccggtat agctggaaat
    10621 gtcgcgcgtc cacagcacgc ggccgttggt tctgtctacc cctgccacac gcccctggaa
    10681 agcaactgca tagaccatgc gcccgtccac caccggctgg ctggtaatgt cggcgatacg
    10741 ttcgatctcg gttgtgccct tggcttgcgc gaccgaggct tcccacagca ccttgccgtc
    10801 gtcgaccttg agcgcgatca gtttgccgcc ggcaaagccg gcataggccg cgccgccatc
    10861 cagcgccacc ccggctgcgc tgcgcaggct tagtgccggt gtgctccgtt cgtaaaccca
    10921 cttgcgcttg ccatcggcgg cactgagccc gaaaatgcgg ctgtcaccgg tccgcaccac
    10981 cactacgccg ctgctgatct tgggcgcgct caatacttcg ctgctgacct tggacttcca
    11041 tagcggcttg ccattctggt cgaaggccag caccatgccc ttgggtgttc ccaccagcac
    11101 caggttatcc ccggcaccga caccccctga gagctgctca ccggcattga tgcgccatac
    11161 ctgtttgccg ctggtggctt ccaggcgaac aatttcgcct tcgccgccag cggcaaacac
    11221 tgccgcccca tccagcgcag ggctaaattc atagaacttg gatttgccta cgttgctgtg
    11281 ccacaacact tttgtcgttg cggtgggctt gaactcgccg agctccattg gaggatcctt
    11341 gggctccggt ccgaagatgc tctcggaaac gctggtcttg agatccgtca gcgaggtgca
    11401 ggcggtcagc agcgcagccg ccagaaacag cacaggtctt tgcaacatca gcttcccagc
    11461 gcgtccagct tgtgttcggt atggtggcgg tagtctcccc ccgcatccag tttggtcagg
    11521 gcttcggcat aggccgcctt ggcttccgct ttcttgcctt gggctgcaag gatgtcgccc
    11581 ttgaggtcgg cgaacaggcc gtcgaacccg gcatcgtgtt tttcgcccaa ggtcttgagc
    11641 gctgcgtcgt actgcttctc gtcaaactgg atggccgcca gttgcaattg ggcaacggcg
    11701 cgaagggcgt cttccttggc atgacccatg gcccactcga gttgcgcttt ggcactcttg
    11761 gcgtcgttgg cggcataatt ggccttggcg gcaatgagtg ccgcacggac agcgtacggg
    11821 gtgcccgcgt atttctctat gatctggccg gagatgctgc gtatgccctt aatgtccttt
    11881 tcttccagtt gggtcagcgc atcgtattgc gctgatgcct gcagtgactg gcggtgctgg
    11941 taggccttcc agccctggta ggcggcaaag gccgccagcg ccaggccgag ccccagcatc
    12001 acggtattgc cgtggcgttt ccaccaggcc ttgagttcat ccaattgttc ctgttcttcc
    12061 agatcgtatg ccatgcttgt ttcctaggtt gccagcccgg ccagatgacg ccggccgggt
    12121 attgaattca acaaattacg tgtatagggc tgctgcgggt cggaataaat cctctccacc
    12181 ttgccttgct ccactatccg cccgcggtgc atcaccacaa tgtcatccgc aacgtggcgc
    12241 actacgcgca ggtcgtggct gatgaagagg tagcttagcc ccatttcctg ctgcaactct
    12301 ttcaacagca acaggatctg cgcctggatc gacacatcca gcgccgatac cggttcgtcg
    12361 cacactacca gcttcggttc cagtaccagc gcgcgtgcga tgccgatgcg ctgacgctgg
    12421 ccgcccgaaa actggtgcgg atacttttcc atgtccgcta cgctcaggcc tacccgctgc
    12481 aacatgcatt cgaccttgtc gcgacgttcg gcagcattac ccagtttgtg aatcagcagg
    12541 ccttcgccaa cgatctcgcc cactttcatg cgcgggttga gggaggcaaa cggatcctga
    12601 aacaccatct gcatgtgccg ccgcgcggca cgcagttggg tgccggtcaa agcggccagg
    12661 tcgcggcctt cgaattgcac ttccccagca tcgaggggct gcaattgtaa cagacaacgc
    12721 gccagggtgg atttgccgct gcccgactcg cccaccaccg caagtgtggc gccctgcctg
    12781 acgctgatac tgacgttgtc cagcgccctg actaggctgg taccaaagcc gaacctgccg
    12841 ctccgctgct tgtatatttt ggtcagtccc ttggcggaga ggatgctgcc gctcatgctg
    12901 ccgcctcgtt cagccaggca tagtcgatcg gcttcagcgg cttcttgcct tccgcatccg
    12961 gtacacagcc cagcaaggcg cgggtgtagg gatgttcggg gttgcccagc accttgtcca
    13021 ctgcgcccat ttccacgatc tcgccacgga acatcaccgc cacctcgtcg gcgatatcgg
    13081 ctaccacgcc gaagtcatgg gtgatgaata gcatgcccat gttcatgcgc tgcttgagtt
    13141 cgttcagcag cgacagaatt tgtgcctgca ccgtcacgtc cagtgcggtg gtcggttcat
    13201 ccgcaattaa catggcgggc tcgcaggcga ttgccatggc aatcatcaca cgctggcgct
    13261 ggccgccgga tagctcatcc gggtagctgc cggcacgctc gggcggaatg ccgacctgtt
    13321 ccagcagtgc ggcgatacgc gcctgcagcg ctttgccgcc gaggccgagg tggaacgcca
    13381 gcgtttcacc gatctggtag ccgacggtca gcaccggatt gagcgaggtc atcggttcct
    13441 ggaaaatcat gccgatacgc ttgccgcgca cgtcctgcat cctggtattg gaatagccgg
    13501 ccaggtcgtt gccctggaac agtacgcgcc cggatcggcg ctgcaacgcg ggcggcagca
    13561 gccccatgac cgaaagtgcg gtcaggctct tgccgctacc ggactcgccg acgatgcagg
    13621 tggtgttgcc ggcttgcagg gtgagcgaga cgttgctgac cagcaggcgt tgtggttggg
    13681 ttgccggcgc aacggtcagg ccttcgatct gcagcaatgc gttcatcagt cgagaagatc
    13741 cacgagttgc gtcaggtcga cgcgggcttg ctcgccggcc tgtcgcaacg gcttcaagct
    13801 caccatctgt gctgctgctt cgtcatcgcc gatgatgacg gcaaagcgcg ctccgctggc
    13861 gtcagccttc ttcatctgtg atttgaagct gccgccgccg cagtgcagta ctgcggccaa
    13921 gccttcattg cgcaattgtt cagctacctg tggcgcccac ctggatgcgg cttcgccggc
    13981 atggacgaca taggcatccg gcgcattggc cggcggcacg aaaccttgct cctgcatcag
    14041 tgccagcagg cgctccaccc ccatggcgta gccgcaggcc ggtgccggtt tgccgccgac
    14101 ctgttcgacc aggctgtcgt agcggccgcc ggcgcatacc gtgccctgcg cgccgaggcg
    14161 tgtggttacc cactcgaaca cggtggcgtt gtaatagtcc aggccgcgga ctagccgcgt
    14221 attgatgcgg tattcgactc cggcgacttt cagcatggcc tgcaaggcct cgaaatgccg
    14281 gagcgaggca tcgtccagat cgtccgccag gctgggcgcg gcttccacca cgggctgcat
    14341 ggccgggttc ttggtgtcga gaatgcgcag cgggttgctg tacaagcggc gcttggcgtc
    14401 ctcgtccagc agttcggtgt gttgctcgaa ataggcaatt aaccgctcgc ggtagcgggc
    14461 gcgggcttcg gcagatccca gcgtgttgat ctccagcgcc acgtcctgca agcccagcag
    14521 cttccacagg cgcgccgtca tgacgatgtg ctcggcatcc atgtccggcc cgtcgtagcc
    14581 cagcgattcc acgcctacct ggtggaactg gcggtagcga cccttctgcg gccgctcgtg
    14641 gcggaacatc gggccgctgt accacaggcg ctgcggggcg ttgtagagca ggttatgctg
    14701 gattacggcg cgtacgcaag acgccgtgcc ttccggccgc agcgtcaggt gttcgccatt
    14761 gagctggtcc tcgaagctgt acatttcctt ctcgacgata tcggtcactt cgccgatgga
    14821 gcggacgaac agatcggtgg attcgacgat aggggtgcgg atttgcgtat agccgtatgc
    14881 acgcagccag tcgcgaatgg tgtcctcgaa aaactcccac agtgcagcgt cggccggcag
    14941 gatgtcgttc atgccgcgga cggcttgtat cgtcttgctc attgttttgt atcgagaatc
    15001 atgaatgtgc gcattttagc atgctgtggc ggggttatcg cgtcgcgtct gtgacgcgcg
    15061 tggcgggttg accatgatcc gctttccggg aggctgccta aattcaaatg gtcgttttct
    15121 cacccatctc gggtgtgcgg gcgttccagc ctagcctgga tctgatcagc ccggcgaact
    15181 gttcggcggt ggcggattcg ccgtggacga cgaaggtttg tcttggggtg tgtttgaagt
    15241 accccagcca ttccagcagc ccagcctggt cggcatgggc ggacaggccg ccgatggtgt
    15301 aaatatcggc acgcacgacg atttcctcgc cgaaaatcct ggccagtttt gccccgtcta
    15361 ccagacgcct ccccagcgtg ccttcagcct gaaagccggt gaagatgatg ctgctttggg
    15421 ggcgattgag gttgtagcgc aaatggtgct tgatgcgtcc cgcatcgcac atgccgctgg
    15481 cggagatgat gatggcgcca gacttgatgt cgttgagcgc catggattcc tccacggtct
    15541 gggtgaagcg gatatccgga agttccttgc gtcgttcttc cccccaggtc aacagtttcc
    15601 gggcttcctg gttgaacagt tcgatgtgat gcatggtaat ttcggtggcc gccagtgcca
    15661 gcggcgaatc gacatagatt ttcaggttgt gcaggcggcg ctgacgggtg aggtcaatca
    15721 acaaatacag gatttcctgg gtgcggccga cggtgaaggc cggcatgatg acgttgccgt
    15781 gcttttccag caaggtgcgc tcgatggcgc ggacgaattc gtccagcgta tcgtccaggt
    15841 tcttgtgcag gcgattgccg taggtcgatt ccaccagcag gatgtcggcg ctgtccactg
    15901 gtgtcgggct gcgcaggatg gggcggccgg gctggccgat gtcgccggag aagacgattt
    15961 tcctgcggtc gccatcttcc cctaaccaga cctcgatgat ggccgagccc aggatatggc
    16021 cggcatccag aaagcagcaa acgacgccgg cccgtggtcc aaattgggag gcgtactcca
    16081 ccggttgcag cagcgccagc gctttttcca cgtcgagcag ggaatagagc ggtgggctgg
    16141 gctcgtcatg accgttgcga tgcggatgac gttcttccca ttctgcttcc ttggcctgaa
    16201 tatgcgcgct gtcgcgcagc atgacgtcca gcagttcaat ggtggccagc gtggcgtaga
    16261 tcggcccgga aaatcctcgc gccgcgaggc gtggcagcat gccggaatgg tcgatgtggg
    16321 cgtgcgtcag cagaatgaaa tcgatggacg cagggtcgaa taatggaaag cgctggtttt
    16381 tcaggtcggc ttcatgtccg ccctggaaca tgccgcagtc gaccagaaag cgtgtcgccc
    16441 cggtttccac cagggtgcag gagccggtga cttcgccggc ggcgccgagg aaagttaatt
    16501 gcataaagtt aattgcatga gggcatcttg tgtttaagaa aactatccgc attttacgat
    16561 aacacgaata gcactggctg atgtagccag caatcgaaac a
//
LOCUS       DAOMFA010000014        16544 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_262_length_16544_cov_4.694948, whole genome shotgun sequence.
ACCESSION   DAOMFA010000014 DAOMFA010000000
VERSION     DAOMFA010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 16544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16544
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_262_length_16544_cov_4.694948"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..434)
                     /locus_tag="PKW44_01475"
     CDS             complement(<1..434)
                     /locus_tag="PKW44_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018410219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RND transporter"
                     /protein_id="HQR50289.1"
                     /translation="MRIDLILLTALLAGCASVPGDAGFTDVRHGVAERLDQSVEWRRG
                     GAEDAEADARVQLLLAQPLSADSAVQVALLNSRALQAEYEELGVMQADVVQAGLLKNP
                     TFGWSHLDGGGPGKTTWGVELDFLGLLLAAPRQGMENVRFKHT"
     gene            complement(514..813)
                     /locus_tag="PKW44_01480"
     CDS             complement(514..813)
                     /locus_tag="PKW44_01480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR50290.1"
                     /translation="MSLVLFCIGTVQAASMGIGVAPVQDAPATHCHDMGGGDSHDHVK
                     PHAVADCHCCPACLPGLAAAITPVIPLTASIVAVAPQAHYLSPIGILPLRPPIST"
     gene            919..3165
                     /locus_tag="PKW44_01485"
     CDS             919..3165
                     /locus_tag="PKW44_01485"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HQR50291.1"
                     /translation="MSSSPTSIRLAIEGMRCAGCVSAVERALRAVPGVVEASVNFADH
                     TAEVSGQAEVIALTTAVAAAGYQASEMVDEEAAEREKEVAEAARYRTLLHKSWFALGV
                     AVPALLFGFPAMLGPMTSENTMPHWLMAWGSLALALLTLAVMVVSGGQFFRGFWNSLR
                     HRHANMDTLIALGTGAAWGYSLAVTLAPEWFPGGTAEPFWDVIPVVIGLVVLGQALEM
                     RARGRTSAAVQRLIGLKPKTARVVRDGQEIDIPLAEVRVGDTVRARPGEKIAVDGVVV
                     DGHSSVDESMLTGEPLPLEKTAGDEITGGTLNKSGSLLFRATRVGKETALARIVEAVR
                     QAQGAKPAIGRLADRVAGVFVPAVLVVAVLAFFLWFNVGPEPHLNYAFVVAVAVLVIA
                     CPCALGLATPMAVMVGVGKAAEYGILIRNGDALQQAGRLTAVVLDKTGTVTQGKPAVT
                     QVIAAPGWEEIRVLHLAASLEAASEHPLAEAVVAAARSRGLELEAAAKFQAAAGQGVW
                     GEVGGHALLLGNLRFIQNFGIDCEGLQDEAAKLTEQAATPIYAAVDGQLAGLIAMADP
                     VKTDSAAAVQALHRMGLKVVLLTGDGLTTAQAVAQQVGIDTVFAEVLPHDKAARIADL
                     QARGEVVAMVGDGINDAIALSRAEVGFAIGTGTDVAIEAADIALMSGSLAGVPNAIAI
                     SRATLRNIRQNLFGAFIYNIIGIPIAAGVLYPLFGLLLNPMIAGAAMAMSSVTVVSNA
                     GRLRNFKP"
     gene            3162..3887
                     /gene="aat"
                     /locus_tag="PKW44_01490"
     CDS             3162..3887
                     /gene="aat"
                     /locus_tag="PKW44_01490"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018076335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HQR50292.1"
                     /translation="MSGYYHLPTGFVAALENSEPFPPLEQALREPNGLLAIGGDLTPP
                     RLLGAYRQGIFPWFNPDNPILWWSPDPRMVLLPENLKVSRSLARRLKKHDYEIRFDTA
                     FREVMQACAASPRPGQDGTWIVPEMVYAYCQLHELGHAHSVETWMDGELAGGLYGVAI
                     GRMFYGESMFHQKTDASKIAFVHLVQFLRREGYGMIDCQMKTEHLERLGAREIPRQRF
                     SAVLAKLVDYPQTPGKWRHDPAE"
     gene            3871..4611
                     /locus_tag="PKW44_01495"
     CDS             3871..4611
                     /locus_tag="PKW44_01495"
                     /EC_number="2.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004057 - arginyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0016598 - protein arginylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginyltransferase"
                     /protein_id="HQR50293.1"
                     /translation="MTLPNDPHLQHLQFYATTAYPCGYLEGRQARSLIAAPHHLIDER
                     VYSELIRQGFRRSGKFAYRPYCEQCQACVPVRLPVAEFRPGRSQRRIWQRHGNLTTRV
                     LELEFIPAHFALYRVYQQARHAGGGMDADGAEQYRNFLAQSNVKTLLVEFREDEQLRM
                     VSVVDCVADGLSAVYTFYDCSDAAASYGTYNVLWLADWCRQLELPYLYLGYWIAESRK
                     MAYKSNFRPLQGLVQGEWKTLEKICRAH"
     gene            complement(4724..7405)
                     /locus_tag="PKW44_01500"
     CDS             complement(4724..7405)
                     /locus_tag="PKW44_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetate--CoA ligase family
                     protein/GNAT family N-acetyltransferase"
                     /protein_id="HQR50294.1"
                     /translation="MANHYLNMLFSPQSIAVFGASDRLDSVGGVVFENLRTAGFAGPV
                     IPINPKRDAVQGVPAFPSLEKAGQQIDLAVICTPAETVPDIIESCGKLGVKGAIILTA
                     GFSEGGEQGKALEGRVLESAARYGLRFIGPNCLGAMRPAIGFNATFNRGMAKPGKLAL
                     VSQSGALCTAILDWASVNDVGFSSVISMGASADVDFGEILDFLLNDPQTDSILLYIEG
                     IRHARSFMSALRAAARVKPVIVVKVGRHASAVKAAQSHTGALVGGDDVFDTALKRAGA
                     VRVQSVMQWFALAKALTSRYRKCGNRLAIVTNAGGPGVIATDRAADLGVAFAELSEVT
                     MERLNAALPASWSHGNPVDIIGDATAERYEQAIKACLEDDGVDGVLAILTPQAMTAPT
                     EVAQRVVAIANNYGKPLLTCWMGETQVNEARQLLSQAKIPHFRSPELAVETFSFIADY
                     YHNQQLLLQTPGPLSDHAEPDVEGARLLIEGVLAERRTVLSEMESKALLSAFRIPVAQ
                     TMVARSPNEALLIAQQLGLPVAMKIHSPDITHKSDVGGVRLDLDSAHAVRGAYHEMIE
                     HVSKLRPDATIDGVAIESMVVKPNGRELMVGIVSDPVFGPVITFGAGGTAVEVLGDRA
                     VALPPLNEHLAGNMIASTRIYKMLKAFRNLPQVDMEALESLLLRVSEMVCELPWIREM
                     DINPLIVDENGLVVADARVIVGPCPEGTEPYAHMAICPYPTPLVSKWQLDDGTDVTIR
                     PIRAEDADMLQDFVVRSMSEESRYYRFMDTLRELSSGMLSRFTQIDYDREMALVATTE
                     EEEGEIMLGVARYTINPDGQSCEFATAVADAWHGRGIGHKLMDSLMAVAHDKGLKVME
                     GEVLASNSGMLDLAAGLGFSVRPHEEDAGLRRMVKQL"
     gene            complement(7435..8484)
                     /gene="hypE"
                     /locus_tag="PKW44_01505"
     CDS             complement(7435..8484)
                     /gene="hypE"
                     /locus_tag="PKW44_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HQR50295.1"
                     /translation="MTKRKSAYTRPLDIERGRIDMNHGSGGRAMAQLVEELFAEALAN
                     EYLAQGNDGAVLPPANGRLVVSSDGHVVSPLFFPGGDIGSLAVHGTINDVAMMGAQPL
                     YLTASFILEEGFPLADLRRIVVSMAEASAAAGVLVVAGDTKVVEQGKGDGVFIATTGI
                     GVVREGVNLSGSLARPGDRILLSGTIGDHGMAILSRREGLEFDAPIVSDSVALHGLVA
                     AMLESGAGLHVLRDPTRGGLATTLNEITRQSGVGMLLQESAIPVAPVVAAASEFLGLD
                     PLYLANEGKLVAICAPADAGRLLEIMQSHPLGGSAAIIGEVTQDPHGFVQMTTRLGGR
                     RIVDWLTGEPLPRIC"
     gene            complement(8487..9611)
                     /gene="hypD"
                     /locus_tag="PKW44_01510"
     CDS             complement(8487..9611)
                     /gene="hypD"
                     /locus_tag="PKW44_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018412527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HQR50296.1"
                     /translation="MKYIDEFRDGALAETIAANIASEVHTGRRYSLMEFCGGHTHAIS
                     RYGLADLLPPSVNMIHGPGCPVCVLPIGRIDQAIRLALQHRVMLCSYGDCLRVPASQG
                     MSLLKARSRGADVRMVYSPADALKLAQAHPERQVVFFAIGFETTTPPTAVVVKQAQSM
                     GLKNFSLICNHVLTPAAMRAILANHGGDRLDGFVGPAHVSTVIGSRPYEVFAHEYGKP
                     VVIAGFEPLDVMQAILMLVRQINRGRAEVENEFTRAVSREGNHKAQALVGEVFRLRDE
                     FEWRGLGVIAHSALALRDAFAEFDAEQRFGLDYHAVPDHKACECGAILRGEKRPEQCK
                     IFGTACTPDTPVGSCMVSSEGACAAHYTYGRFKNVAVAAG"
     gene            complement(9608..9838)
                     /locus_tag="PKW44_01515"
     CDS             complement(9608..9838)
                     /locus_tag="PKW44_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HQR50297.1"
                     /translation="MCLAIPVRVETLNGTESAIVNLVGVRKEISLALVDDVAVGDYVI
                     VHVGFALQKIDPDEAAQTLKLFAELGVTESGV"
     gene            complement(9840..11021)
                     /locus_tag="PKW44_01520"
     CDS             complement(9840..11021)
                     /locus_tag="PKW44_01520"
                     /inference="COORDINATES: protein motif:HMM:NF036810.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HQR50298.1"
                     /translation="MAATGAGGDGRVAGAAGYRMNGLVAANIRLSHEAQPVLATGAWL
                     SNTLCVTHGRQAWVSAVVGDLATPEACLAHEATARALLQALETRPAAIAHDLHPDFHS
                     SRHAAYLAAQLDVPLIAVQHHHAHIAALAAEHGVEQPLLGLALDGVGLGTDDIPWGGE
                     LLLVDGAKFSRLGCFRPLPLPGGDRAAQEPWRLAAAVLHEAGRGMEITTRFHAQPGAA
                     TVAAMLARDLNCPRTSSAGRVFDAAAGLLGICTVMAFESQAARLLEQAAASFMRNNGR
                     PLALEDGWHIDRNGRLDLLPLLTTLAGTEDTGYGAALFHTTLAAALAEWVVDAARHSG
                     VTVVACGGGCFFNEILSSELQRLLSAENVRLLTAKSMQPGDSAISLGQAWVAIHRLES
                     L"
     gene            complement(10961..11842)
                     /gene="hypB"
                     /locus_tag="PKW44_01525"
     CDS             complement(10961..11842)
                     /gene="hypB"
                     /locus_tag="PKW44_01525"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018227504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HQR50299.1"
                     /translation="MCTTCGCGGTETHINGQAMHEHVGQDGRVYRHVHAHPHDHDHDY
                     LHDHGHPHSGIGAVHSHVGRVTQSRMVRIEQDILAKNNAHAGKNREFFKERGILALNL
                     VSSPGSGKTALLVRTIAALDGQPVAVIEGDQQTSNDAERIRASGAPAIQINTGKGCHL
                     DAQMVEQALPQLAPVHNSLLFIENVGNLVCPAAFDLGEAAHVAILSVTGGEDEPLKYP
                     DMFRAARLMLLNKCDLLPYLQFNVEQTIENAHRVNPAIEVIQISVASGAGMQDWLAWL
                     QREQEAMAASQGRPVTA"
     gene            complement(11849..12196)
                     /gene="hypA"
                     /locus_tag="PKW44_01530"
     CDS             complement(11849..12196)
                     /gene="hypA"
                     /locus_tag="PKW44_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HQR50300.1"
                     /translation="MHEMSLAEEVLRIIEDAASDQGFRRVRTVILEIGKLAAVEPEAM
                     RFCFDAVVSGTLAEGAALEIEETAGEGWCAQCMATVAMHEELGACPRCGGYRVQVTGG
                     TGMRVKELEVEQE"
     gene            complement(12200..12778)
                     /locus_tag="PKW44_01535"
     CDS             complement(12200..12778)
                     /locus_tag="PKW44_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018077110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HQR50301.1"
                     /translation="MRNILKVLLGAALCLPVVALAHTGAGSVTGFFSGFAHPLMGVDH
                     LLAMVAVGLWAAQIGGAARWQVPTAFVTLMLLGGALGMSGFSLPYIESGVVGSVMLLG
                     LLIAVSCRPSNAVSMAIVSFFALFHGHAHGAEMPVLLGAIAYSCGFALTTASLHAVGL
                     ALGGAVRGGGHDRLTRLAGAGIALAGAWLAVA"
     gene            complement(12804..13301)
                     /locus_tag="PKW44_01540"
     CDS             complement(12804..13301)
                     /locus_tag="PKW44_01540"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HQR50302.1"
                     /translation="MAAADVLVLAVGNESRGDDALGPLLLRRLQTWLEVHGCADRVEL
                     LEEFQLQVEHTMDIAGRRLVLFVDAGVQTRAPYVFCRAVPKRMEGHTSHAVAPEMLLG
                     IYDMVHGEAPPPAFVLCVAGREFELGEPLTAEAQENLTQAFDLVCGLLEQPDPAAWQG
                     VAEVG"
     gene            complement(13304..14785)
                     /locus_tag="PKW44_01545"
     CDS             complement(13304..14785)
                     /locus_tag="PKW44_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe hydrogenase subunit alpha"
                     /protein_id="HQR50303.1"
                     /translation="MNNLETAEHPEKLRRVAIDPVSRVEGHGKVTLLLDENNKVHQVR
                     LHIVEFRGFEKFIQGRPYWEVPVMVQRLCGICPVSHHIAASKAMDMMLGVEHLTPTAE
                     KNRRLMHYGQIMQSHALHFFHLCSPDLLFGFDSEVARRNIVGVAAEYPDIAKQGVLLR
                     KFGQEVIRMTAGKRIHGTGAIPGGVNKSLSREERDALLAEVYRMIAWSREAVHLVRRL
                     FEQNLQEYNAFGRFRAHQLSIVRADGAMDLYHGGLRARGMDGDIIFDHYDYRHYWDVI
                     SEDVKSWSYMKFPFLRSLGPETGWYRVGPLTRVVNCDFIPTSFAEKERREFLAFDDGR
                     ATGSTLGFHWARMIEMLHAVESIKDLLHDDDIMGEQLMATGERGGRGVGVIEAPRGTL
                     IHHYRVDENDLVIRANLIVSTTHNNQAMNEAVRRVAMQYLDGNEITEGLLNHIEVAIR
                     AFDPCLSCATHALGKMPLEVELLDMEGNLVDRLMRSSDGVITA"
     gene            complement(14794..15345)
                     /locus_tag="PKW44_01550"
     CDS             complement(14794..15345)
                     /locus_tag="PKW44_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP oxidoreductase"
                     /protein_id="HQR50304.1"
                     /translation="MDAPVKKKLKLATATLAGCFGCHMSFLDIDERLFDLVELVEFDR
                     SPLTDIKQVGQCDIGLIEGGLCNAENVHVLREFRQHCKILVAVGACAINGGLPAQRNP
                     IPLSVILNEIYLAEAGLAHGGIPNDPELPLPLDKVHPIHEVVKVDYFLPGCPPSGDAI
                     WRFLTDLLAGREPELGHALMHYD"
     gene            complement(15347..16063)
                     /locus_tag="PKW44_01555"
     CDS             complement(15347..16063)
                     /locus_tag="PKW44_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004174413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HQR50305.1"
                     /translation="MSKTFTLDGKDVPFEDGQTIIQAALDAGHYIPHLCHHPEFKPHG
                     SCKLCTVTVNGRQTASCTMQATEGMVVESETEELNAERVALTQMLFVEGNHFCPSCEK
                     SGDCQLQATAYHLGMTTAHYDHFFPNRPVDASHPDFLLDFNRCILCSLCVRASRDVDG
                     KNVFALAGRGIQSHLIVNAKSGKLADTDFSALDMAAQVCPVGVILPKRRGFAVPIGER
                     TFDRQPIDVYVDTRLTHDED"
     gene            complement(16060..>16544)
                     /locus_tag="PKW44_01560"
     CDS             complement(16060..>16544)
                     /locus_tag="PKW44_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HQR50306.1"
                     /translation="PSGVTVSRQEFDRILGFADIGTPGASTVFDTSRDMFEVARNFTH
                     FFAHESCGFCTPCRVGTSLLRNLMDKLHDGHGSPYDLEEIEKLNKLLLSMSHCGLGHT
                     ACNPVLDTIAKFRPAYETRMLHRDFTPAFDLDYALQAARQMTGRDDSGAHLHNVEENT
                     "
BASE COUNT         3156 a         5367 c         5038 g         2983 t
ORIGIN      
        1 gtgtgcttga agcgcacatt ctccatgccc tgccggggcg cggccagcag caggccaaga
       61 aaatccagct ccactcccca agtggtcttg cccggcccgc cgccgtccag atgactccag
      121 ccgaaggtcg ggttcttcag caggccagcc tggacgacat cggcctgcat tacgcccagt
      181 tcctcgtatt ccgcctggag cgcacggctg ttgagcagcg ccacctgcac cgcactatcg
      241 gcggagagcg gctgtgccag cagcagctgc acgcgcgcgt cagcctcggc atcctccgcg
      301 ccgccgcggc gccattcgac cgactggtcg aggcgctcgg caacgccgtg ccggacgtcc
      361 gtaaagccgg catccccggg cacactggca catccggcca gtagtgccgt caataaaatc
      421 aggtcaattc gcatgaaaac tcccaatgca catggccgcc atcccgcaag ggtggcgcta
      481 aacccggcta atgccgggcg ttccggaaag ggatcaggtg gaaatgggag gccgcaacgg
      541 caggatgccg atgggcgaga ggtagtgcgc ttgtggcgcc acagcaacaa tggacgccgt
      601 caggggaata accggtgtga ttgccgctgc caaccccggc aggcaggccg ggcagcaatg
      661 gcaatccgct acagcgtggg gtttgacgtg atcatggctg tctccgccac ccatatcatg
      721 gcaatgcgtc gccggcgcat cctgcaccgg ggcgacgccg atgcccatgg aagccgcctg
      781 caccgtgccg atgcagaaca ggaccagcga caaggctatg aaaaagcgat ggaaacgcat
      841 aagctcattg aaacacaaca taaattccgg agcaagcctg ccttgcactc taccaatggg
      901 cgcagtacgc tagattccat gtcgtcatct cccacctcca ttcgccttgc gatcgaaggc
      961 atgcgctgcg ccggctgcgt ttctgccgtg gaaagagcct tgcgtgctgt gccaggggtg
     1021 gtggaagcca gcgtcaattt cgctgaccac acggctgaag tgagcggtca ggcggaggtc
     1081 atagcgctca ccacagcggt tgcggcggcg ggctaccagg ccagcgagat ggtggacgag
     1141 gaagccgccg aacgcgaaaa agaggtagca gaagcggcgc gctaccgcac cctgctgcac
     1201 aaatcgtggt tcgcactggg cgttgccgtg cccgccttgc tgttcggctt cccggccatg
     1261 ctgggcccaa tgacatcaga aaacaccatg ccgcactggc tgatggcctg gggttcgctg
     1321 gcgctcgcgc tactgacgct ggcggtgatg gtggtctccg gtgggcagtt cttccgcggc
     1381 ttctggaatt cgctgcgcca ccgccacgcc aacatggata cgctgatcgc gctcggcacc
     1441 ggcgcggcgt ggggctattc gctggcggtg acgctggcgc cagagtggtt ccccggcggt
     1501 acggcggaac cgttctggga cgtgattccg gtcgtaatcg ggctggtggt tctgggccaa
     1561 gcgctggaga tgcgagcgcg tggacgcact tcggcagcag tacagcggct gatcggcctc
     1621 aagccgaaaa cggcgcgcgt ggtgcgcgac ggccaggaaa tcgacatccc tctggccgag
     1681 gtgcgcgtgg gcgacaccgt gcgtgccagg ccgggtgaaa agatcgcggt ggacggcgtg
     1741 gtggtggacg gccattccag cgtggacgaa tccatgctga ccggcgagcc gctgccgctg
     1801 gaaaagaccg cgggcgacga gattactggc ggcacgctca acaaatccgg cagcctgctg
     1861 ttccgggcaa caagagtagg caaggaaacc gcgctggccc gcatcgtcga ggcggtacgc
     1921 caggcgcaag gcgccaagcc cgccatcggc cgcctggccg accgcgtcgc cggggtattc
     1981 gtcccggcag tgctggtggt cgctgtattg gcattttttc tctggttcaa tgtcggcccg
     2041 gaaccgcacc tcaactacgc ttttgtggtt gcggtggcgg tgctggtcat cgcctgcccc
     2101 tgtgcactgg gactggcgac gccgatggcg gtgatggtag gcgtgggcaa ggcggcggaa
     2161 tacggcattc tgatccgcaa cggcgacgcc ctgcagcagg ccgggcggct gaccgcggtg
     2221 gtgctggaca agaccggcac cgtgacccaa ggcaagcccg cggtcacgca agtcatcgcc
     2281 gcgccgggct gggaagaaat ccgggtgctg catctggccg ccagcctgga agcggcttcc
     2341 gagcatccgc tggcagaggc ggtggtagcc gcggcacgca gccgcgggct ggagctagag
     2401 gcggcggcga agtttcaggc cgccgccgga cagggcgtgt ggggggaagt cggcggacat
     2461 gcactgctgc tcggcaacct gcgcttcatt cagaacttcg gcatcgactg cgaggggctg
     2521 caggatgagg ctgcgaagtt gacggaacag gccgcgacgc ccatctatgc ggccgtggat
     2581 ggtcagctgg ccggcttgat cgcgatggcc gacccagtca aaacggattc cgccgctgcg
     2641 gtgcaggcgc tgcaccggat ggggctgaag gtcgtgctgc tgaccggcga cggcctgacc
     2701 acggcgcaag cggtggcgca acaggtcggg atagacaccg tgttcgccga ggtgctgccg
     2761 catgacaagg ccgccaggat tgccgatttg caggcgcgag gcgaagtggt ggccatggtg
     2821 ggtgacggca tcaacgacgc catcgcgctc tcccgcgccg aggtggggtt cgcgataggc
     2881 acgggaaccg atgttgccat cgaagccgcg gacatcgccc tgatgtccgg ctcgctggcg
     2941 ggggtgccaa acgccatcgc catctcgcgc gccacgctgc gcaatatccg gcagaacctg
     3001 ttcggcgcct tcatctacaa catcatcggc attccgatcg ccgccggcgt gctgtatccc
     3061 ctgttcggcc tgctgctcaa ccccatgatc gccggcgcgg cgatggcgat gtcgtccgtc
     3121 accgtggtca gcaacgccgg gcgactcaga aacttcaagc catgagcggc tattaccacc
     3181 tgcctaccgg attcgtcgcg gcgctggaaa acagcgagcc gtttccgccg ctggaacagg
     3241 ccctgcggga acccaacggc ctgctcgcca tcggcggcga cctgacgcca ccaaggctgc
     3301 tgggcgccta ccggcaaggc atcttcccct ggttcaaccc cgacaacccc atcctgtggt
     3361 ggagtccaga cccgcgcatg gtgctgttgc cggaaaatct gaaggtttcc cgctcgctcg
     3421 cccgacggct gaaaaagcac gattacgaga ttcgcttcga caccgctttc cgcgaagtca
     3481 tgcaggcttg tgccgcctca ccgcgaccgg gccaggacgg tacctggatc gtgccggaaa
     3541 tggtatatgc ctattgccag ctgcacgagt tgggccatgc gcactcggtc gaaacctgga
     3601 tggatggcga actcgccggt ggactgtacg gcgtggccat cggccgcatg ttctatggcg
     3661 aatccatgtt ccaccagaaa accgatgcct ccaaaatcgc tttcgtacat ctggtgcagt
     3721 tcctgcgacg agagggctac ggcatgatcg actgccagat gaagactgag catctggaac
     3781 gcttaggcgc gcgggaaatt ccacgccaga gattctctgc cgtactcgcc aaattggtag
     3841 actacccgca aactcccgga aaatggcgcc atgaccctgc cgaatgatcc ccacctgcag
     3901 cacctccagt tctatgccac cacggcttac ccgtgcggct atctggaggg caggcaggcg
     3961 cgctcgctga tcgcggcgcc gcaccacctg atcgacgagc gcgtttacag cgagctgata
     4021 cgccagggtt tccgccgcag cggcaagttc gcctaccggc catactgcga acagtgccaa
     4081 gcctgcgtgc cggtacggct gccagtcgcg gaattccggc ccggccgcag ccagcgccgc
     4141 atctggcaga ggcatggcaa tctcaccacc cgcgtgctgg aacttgaatt cataccggcg
     4201 cattttgcgc tctaccgcgt ctaccagcag gcacgccatg cgggcggcgg catggatgcc
     4261 gacggcgccg agcagtaccg gaattttctg gcgcaaagca acgtgaaaac gctgctggtc
     4321 gaatttcgcg aagacgaaca gttgcgcatg gtgagcgtgg tggactgcgt agctgacggt
     4381 ctgtcggcag tctatacctt ctacgattgc agtgacgcgg cagcctccta tggcacctac
     4441 aacgtgctgt ggctggctga ctggtgccgc cagctggaac tgccttatct ctatctgggt
     4501 tactggatcg cggaatcgcg caagatggcc tacaagagca acttccgtcc gctgcaagga
     4561 ctggtgcagg gcgaatggaa aacgttggaa aaaatatgcc gagcccactg aactttccaa
     4621 aaatacccta gtcctatggg tgtcatctcc atgagcaacc ggcgttttgg ctgatatttt
     4681 tatttcatgc caaaacgccg ttcttgcttt tattccactg catttacaac tgtttcacca
     4741 tccgcctcag gcccgcatcc tcctcgtgcg gccgcacgct gaatccaagc ccggccgcca
     4801 gatccagcat gccggaattg ctcgccagca cctcgccttc catcactttc aggcccttgt
     4861 cgtgcgccac tgccatcagc gagtccatca gcttgtggcc gataccgcgg ccgtgccagg
     4921 catccgccac cgcggtggcg aattcgcagc tctgtccgtc cgggttgatg gtgtagcgcg
     4981 ccacgccgag catgatttca ccctcctctt cctcggtcgt ggccactagc gccatttcac
     5041 ggtcgtagtc gatctgcgtg aaacgtgaca gcatgcccga gctgagttcg cgcagggtgt
     5101 ccatgaagcg gtagtagcga ctttcctccg acatcgagcg caccacgaaa tcctgcagca
     5161 tgtccgcatc ctcggcgcgg atcgggcgga tagtgacatc ggtaccatcg tccagctgcc
     5221 acttcgaaac aaggggcgtc ggataagggc agatcgccat gtgcgcatag ggctcggtgc
     5281 cctccgggca tggcccgaca atgacgcgtg catctgccac caccagacca ttctcgtcca
     5341 cgatcagagg gttgatgtcc atctccctga tccagggcag ttcgcacacc atttccgaga
     5401 cacggagcaa caggctttcc agcgcctcca tatccacctg cggcaggtta cggaaggcct
     5461 tgagcatttt gtagatgcgc gtgctggcaa tcatgttgcc ggccaggtgc tcattgagcg
     5521 gcggtagcgc cacggcacgg tcgcccagca cttccaccgc ggtacccccg gcgccgaaag
     5581 tgatcacggg accgaatacc gggtcggaca cgatgccgac catcagctcg cggccgttgg
     5641 gcttgaccac catggactcg atagcaacgc catcgatggt agcgtctggg cgcagtttgc
     5701 tgacatgctc gatcatctca tgataggcgc cgcgtactgc atgggcgctg tcgaggtcga
     5761 ggcgcacgcc gccgacatcg ctcttgtgcg tgatgtccgg cgagtggatt ttcatcgcca
     5821 ccggcagccc caactgctgg gcgatcagca gcgcctcatt cggcgaacgc gccaccatgg
     5881 tctgcgccac cgggatgcgg aaggccgaca gcagcgcctt ggattccatt tccgacagca
     5941 ccgtgcggcg ctcggccagc acgccctcga tcagcaggcg cgcgccctcg acatccggct
     6001 ccgcatggtc tgacagcggc cccggcgttt gcagcagcaa ttgctggttg tggtagtagt
     6061 cggcaatgaa ggaaaaggtt tccaccgcca gttccggcga gcggaaatgc gggattttgg
     6121 cctgggacag caactggcgc gcctcgttga cctgcgtctc gcccatccag caggtcagca
     6181 gcggcttgcc gtagttgttg gcgatggcga cgacgcgctg cgccacctcg gtcggcgcgg
     6241 tcatggcctg cggcgtcagg atcgccagca ccccgtccac accgtcatcc tccagacagg
     6301 ccttgattgc ctgctcataa cgctcggcgg tagcgtcgcc gatgatgtct accggattcc
     6361 cgtgcgacca gcttgccggc agagcggcat tgagcctctc catggtgacc tcggacaact
     6421 cggcgaaggc tacgcccaaa tcggccgcgc ggtcggtcgc gatcaccccc ggcccgccag
     6481 cgttggtcac gatggcgaga cggttgccgc acttgcggta gcgcgaagtc agcgccttgg
     6541 ccaaagcaaa ccattgcatc acgctttgca cccgcaccgc gccggcacgc ttcaaggcgg
     6601 tatcaaacac atcgtcgccg cccaccagcg ccccggtgtg cgactgtgcc gccttgaccg
     6661 cggaggcatg gcgcccgacc ttgaccacga tcaccggctt cacccgcgct gccgcgcgca
     6721 acgcgctcat gaagctgcgg gcatggcgga tgccctcgat gtacaaaaga atgctgtcgg
     6781 tctgcgggtc gttgagcagg aaatccagga tttcgccaaa atccacgtcg gccgaggcgc
     6841 ccatggaaat cacgctggaa aagccgacgt cgttcacgct ggcccagtcc aggatggcgg
     6901 tacacagcgc accggactgc gagaccagcg ccagtttccc cggcttggcc atgccacggt
     6961 tgaaggtggc gttgaaaccg atggccggcc gcatggctcc caggcagttt ggcccgataa
     7021 agcgcagccc atagcgcgcc gcagattcga gcacccgccc ctccagcgcc ttgccctgct
     7081 cgccgccttc gctgaagccg gcggtgagga tgatcgcgcc cttgacaccc agcttgccgc
     7141 aggattcgat gatgtccggt accgtttctg ccggcgtgca aatcacggcc agatcgatct
     7201 gttgtcccgc cttttccagc gaagggaaag cggggacgcc ctgcaccgca tcgcgcttgg
     7261 gattgatggg gatcaccggc ccggcgaaac ctgccgtgcg caggttctcg aacaccacgc
     7321 cgccgacgga atccaggcgg tcgctggcac cgaatacggc gatggattgg ggggaaaaca
     7381 gcatattcaa ataatggttg gccatgtttg gtccttgaat tttgaggttg tgtttcagca
     7441 tattcttggc agtggctcgc cggtaagcca gtcgacaatg cgacgcccgc ccagacgggt
     7501 cgtcatttga acgaaaccgt ggggatcctg cgtgacttcg ccaatgatcg cagcactgcc
     7561 gcccaaagga tgtgactgca tgatttcaag caggcgcccg gcgtcggcag gcgcgcagat
     7621 ggcgaccagc ttgccttcat tggccagata gagcggatcc aggccaagaa actcagaggc
     7681 cgccgccacg acgggggcga cgggaattgc cgattcctgc agcaacatgc cgacgccgga
     7741 ctggcgggta atttcgttga gcgtagtggc gagtcctccc cgcgtcggat cgcgcagcac
     7801 atgcaggccc gcaccgcttt ccagcatcgc cgcgaccagc ccatgcaggg caacactgtc
     7861 ggaaacgatc ggcgcatcga attccagacc ttccctcctt gaaagaatcg ccatgccgtg
     7921 gtcgccgatc gttccggaaa gcagaatgcg atcaccagga cgcgccaggc taccggaaag
     7981 gttcacccct tcacgaacga caccgatgcc ggtcgtggca atgaatacgc catcgccctt
     8041 gccttgctcg accaccttgg tatcgcctgc gaccaccagc acgccggctg ccgcagaggc
     8101 ctcagccatt gacacgacga tgcgccggag atcggccagc gggaacccct cctcgaggat
     8161 gaaggaagcg gtcagataga ggggctgcgc gcccatcatc gcgacatcgt tgatcgtgcc
     8221 gtgcaccgct agcgagccga tgtcgccccc cgggaaaaac aatggcgaca cgacatggcc
     8281 gtcgctggat accaccagcc ggccattggc gggcggcaag acagcgccat cgttgccctg
     8341 cgccaaatat tcgttggcga gcgcttcggc aaacagttcc tcgaccagct gcgccatcgc
     8401 cctgcccccg ctgccgtggt tcatgtcgat acggccgcgt tcaatgtcga gcgggcgggt
     8461 ataggctgat ttgcgttttg tcatctctac ccggccgcaa cggcaacgtt cttgaatcgc
     8521 ccataggtat agtgagcggc gcaggcacct tccgacgata ccatgcatga gccgaccggg
     8581 gtgtccggcg tacatgccgt gccgaagatc ttacattgct cggggcgctt ttccccgcgc
     8641 agaatggccc cgcattcgca ggctttgtga tctggcaccg catgatagtc gagcccgaag
     8701 cgctgctcgg catcgaattc ggcaaaggcg tcgcgcaatg ccagggcgct gtgtgcaatc
     8761 acgcccagtc cgcgccattc gaactcatcg cgtagtcgga acacctcgcc gaccagtgcc
     8821 tgcgccttgt ggttgccttc ccggcttacc gcgcgcgtga actcgttttc cacttctgct
     8881 cgcccccggt tgatctggcg caccagcatc aggatggcct gcatgacatc cagcggctcg
     8941 aaaccggcga tcacgaccgg tttgccatac tcgtgcgcga acacctcgta gggccggcta
     9001 ccgatcaccg tagacacgtg cgccgggccg acaaaaccgt cgagccggtc acccccatga
     9061 ttggcgagga ttgcccgcat cgcggccggg gtcagcacgt gattgcagat gagagaaaag
     9121 ttcttcaggc ccatggactg cgcctgcttg acgacgaccg cggtaggtgg cgtcgtggtc
     9181 tcgaaaccga tggcgaagaa caccacctgt cgttccggat gcgcctgtgc cagcttgaga
     9241 gcatcggcag gagaatacac catgcgcaca tcggcacccc gcgacctggc cttgagcagc
     9301 gacatgccct gtgaggcggg cacgcgcaag caatctccat agctgcagag catgacccgg
     9361 tgttgcagcg cgagccggat ggcctgatca atacggccga tcggcagcac gcacaccggg
     9421 cagcccggcc cgtggatcat gttgactgat ggcggcagca agtcagccag accatagcgg
     9481 gaaatcgcgt gagtgtgccc gccgcagaac tccatcaggc tgtagcggcg tccagtatgc
     9541 acctcgctgg cgatattggc agcgatggtt tcagccagcg cgccgtcgcg aaactcgtcg
     9601 atgtatttca cacgccgctt tccgtcacac ccagctcggc gaacagcttg agcgtctgcg
     9661 cggcttcatc cgggtcaatt ttctgcaagg cgaaacccac gtgcacgatc acgtaatcac
     9721 cgacggcgac atcgtccacc agcgccagcg agatctcctt gcgcacaccg accagattga
     9781 cgatggcact ctcggtacca ttgagcgttt ccacgcgaac aggaatagcg agacacatgt
     9841 cagagggact ccagtcgatg tatggcgacc caggcctgac ccagggaaat ggccgagtcg
     9901 ccgggctgca tggatttcgc cgtcagcagg cggacatttt cagccgaaag caagcgctgc
     9961 aactctgacg acagaatctc gttgaaaaaa caaccgccac cacaggccac gacggtgaca
    10021 ccagaatggc gcgcggcgtc cactacccac tcggccagtg ccgcggccag ggtcgtgtgg
    10081 aatagcgcgg cgccgtaccc agtgtcctcc gtccccgcca gggtggtcag taacggcagc
    10141 aagtcgagcc gtccattccg gtcaatgtgc cagccatcct ccagcgctag aggccgcccg
    10201 ttgttccgca taaagcttgc agccgcctgt tccaacagcc tggcggcctg tgactcaaac
    10261 gccatcaccg tacatatgcc tagcagaccg gctgccgcgt cgaatacgcg gccggcgctt
    10321 gacgtgcgtg gacaattgag gtcgcgtgcc agcatcgccg cgaccgtggc ggcgccgggc
    10381 tgcgcatgaa atcttgtcgt gatctccatg ccgcgcccgg cttcgtgcaa cactgcggcc
    10441 gccaggcgcc atggctcctg tgcggcccgg tcgccgccag gcaatggcaa cggcctgaaa
    10501 caaccgagac ggctgaattt cgctccgtcc accagcaaca actcgccgcc ccagggaatg
    10561 tcatctgtac ccagccccac gccatccagc gccaaaccga gcagcggctg ttctacccca
    10621 tgctccgcgg ccagggcagc gatatgggcg tggtgatgct gcacggcaat caagggcaca
    10681 tcgagttggg cggccaagta cgcagcatgg cggctggaat ggaaatccgg gtgcagatcg
    10741 tgagcgatgg cggctggtct ggtctccagt gcttgcagca gagcgcgggc tgtcgcctca
    10801 tgcgccaggc aggcttccgg cgttgccaga tccccgacca ccgcgctgac ccaggcttgc
    10861 cggccatgcg tgacgcacag cgtgttgctg agccaagctc ccgtcgccaa aaccggctgc
    10921 gcttcgtgcg acaaccggat gttggccgca accaatccgt tcatgcggta accggccgcc
    10981 cctgcgacgc ggccatcgcc tcctgctccc gttgcagcca agccagccaa tcctgcatgc
    11041 cggcaccgct ggctaccgaa atctggataa cttcgatagc gggattcacg cgatgggcat
    11101 tttcgatagt ctgctccacg ttgaactgga gatacggcag caagtcacac ttgttgagca
    11161 gcatcaggcg cgccgcgcgg aacatgtcag ggtacttgag cggctcgtcc tcgcctcccg
    11221 tgacagaaag gatggctaca tgggccgcct cgcccagatc gaacgccgcc gggcagacca
    11281 gattgccgac gttttcgata aacagcagag aattgtgcac cggagcgagt tgaggcagcg
    11341 cctgctccac catctgcgca tcgagatggc agcccttgcc ggtattgatc tgaatggctg
    11401 gggcgccgct ggcgcggatg cgttcggcat cgttgctggt ctgctggtca ccttcgatga
    11461 cggctaccgg ttgcccgtcc agggcggcga tcgttctcac cagtagcgcc gtcttgcccg
    11521 accccgggct ggaaaccagg ttcagtgcga gaatgcctct ttccttaaaa aactcccgat
    11581 ttttgccggc atgggcattg ttcttggcga ggatgtcttg ctcgatccgt accatgcgcg
    11641 actgcgtcac cctcccgaca tggctatgca cggcaccgat gccggaatgc ggatggccgt
    11701 ggtcatgcag ataatcgtgg tcatgatcgt gcgggtgggc gtggacatgg cggtacaccc
    11761 ggccatcctg cccgacatgc tcgtgcatgg cctgcccatt gatgtgcgtt tccgtgccgc
    11821 cgcagccaca agtggtacac atgattgcct actcctgttc gacttccagt tccttgaccc
    11881 gcatcccggt gccgccggtc acctgtacgc ggtaaccacc gcatctgggg caggcgccca
    11941 attcctcgtg catggccaca gtcgccatgc actgcgcgca ccagccttct cccgcagttt
    12001 cctctatctc cagcgcagcg ccttccgcca gcgtgccgct caccaccgca tcgaagcaga
    12061 aacgcatcgc ctccggttcg accgcggcaa gcttgccgat ctccagaatc actgttctga
    12121 cgcgccggaa accctgatcg cttgccgcat cctcaatgat ccgcagcacc tcttcggcca
    12181 gagacatttc atgcatcgat caagccaccg ccagccaagc tcccgccagc gcgatgccgg
    12241 cgccagcaag gcgcgtcagc ctatcatgcc caccccctcg caccgcacca cctagggcaa
    12301 gcccgacggc gtgcagcgat gcggtcgtca gcgcaaaccc gcaactgtag gcaatcgcac
    12361 ccagcaaaac cggcatttca gcaccgtgcg cgtgcccgtg aaacaaagca aaaaagctga
    12421 cgatcgccat gctcacggcg ttcgaagggc ggcaggaaac agctatcagc agcccgagca
    12481 gcatgaccga accgacgact ccagattcga tataaggcag actgaacccg gacattccga
    12541 gcgcgccgcc aagcagcatg agtgtgacga aagccgtggg gacctgccat ctggccgccc
    12601 cccctatttg agcggcccac agccccaccg ccaccatcgc cagcaagtgg tcaaccccca
    12661 tcaagggatg agcgaagccg gagaaaaacc cagtgactga cccggcaccg gtatgggcca
    12721 gcgccaccac cggcaggcat aacgccgcac ccagtaagac cttcaggata ttacgcatca
    12781 caacactcct cttccaattc gattcaacca acttccgcaa caccctgcca cgctgccggg
    12841 tctggctgtt ccagcaggcc gcagaccaag tcaaacgctt gcgtcaggtt ttcctgtgcc
    12901 tcagccgtca atggttctcc aagctcaaat tcccggccgg ccacgcaaag gacaaatgcc
    12961 ggggggggtg cttctccatg caccatgtca taaatgccca gcagcatttc aggcgcgaca
    13021 gcatggctgg tatggccctc catgcgcttc ggcaccgcac ggcagaacac ataaggtgcc
    13081 cgcgtttgaa cgccggcatc gacgaacaac accagtcgcc ggccggcaat atccatcgta
    13141 tgttcgacct gcaactggaa ctcttccagc aattcgactc gatccgcaca cccatgcact
    13201 tccagccagg tctgcagcct gcgcagcagc aacggaccca gcgcatcgtc gccacggctt
    13261 tcgttgccga ccgccagaac cagtacgtcc gccgccgcca atttcaggcc gtaatcacgc
    13321 cgtcagacga tctcatcagg cgatccacca ggtttccttc catatccagc agttcgactt
    13381 ccagcggcat tttccccaac gcatgcgtag cgcaggacag acatgggtcg aaggcgcgga
    13441 tggcgacctc gatgtggttg agcagcccct cggtgatttc gttaccatcc agatactgca
    13501 tcgccaccct gcgtaccgcc tcgttcatcg cctggttgtt atgggtagtg gagacgatca
    13561 ggttggcgcg gatcaccagg tcgttctcgt ccacgcggta atggtggatc aaggtaccgc
    13621 gcggcgcctc gatcacgccg acgccgcggc cgccacgctc gccggtggcc atcagctgct
    13681 ctcccatgat gtcgtcgtcg tgcagcagat ccttgatgga ttccaccgca tgcagcatct
    13741 cgatcatgcg cgcccagtgg aagcccagcg tcgaaccggt ggcacggccg tcgtcgaagg
    13801 ccagaaactc gcgccgctcc ttctcggcga acgacgtcgg gatgaaatca cagttgacca
    13861 cacgggtgag cgggccgacg cggtaccagc cggtttccgg acctaaactg cgcaggaacg
    13921 ggaacttcat gtagctccag gatttcacgt cctcggaaat cacgtcccag taatggcggt
    13981 agtcgtagtg gtcgaagatg atgtcgccat ccatgccgcg cgcgcgcaag ccgccgtggt
    14041 aaagatccat ggcgccgtcg gcgcggacaa tgctcaattg gtgcgcgcgg aaacggccga
    14101 aggcgttgta ttcctgcaaa ttctgctcga acagccggcg caccaggtgc accgcctcgc
    14161 ggctccacgc gatcatccgg tagacctcgg cgagcagcgc gtcgcgctcc tctcggctca
    14221 aggatttgtt caccccgccg gggatagcac cggtgccatg gatgcgcttg cccgccgtca
    14281 tgcggatcac ttcctggcca aacttgcgca gcagcacgcc ctgtttggcg atgtccgggt
    14341 attcggccgc cacgccgaca atgttgcgcc ttgccacttc ggagtcgaag ccgaacagca
    14401 gatcgggcga gcacaggtgg aaaaaatgca gggcgtgcga ctgcatgatc tggccgtagt
    14461 gcatcagcct acggttcttc tccgccgtcg gcgtcaggtg ctcgacgccg agcatcatat
    14521 ccatggcctt ggaggcggca atgtggtgcg acaccgggca gatgccgcac aggcgctgca
    14581 ccatcaccgg gacttcccaa tacggccgac cctggatgaa tttttcgaag ccgcggaatt
    14641 cgacgatgtg caggcgtacc tggtgcacct tgttgttttc gtccagcagc aaggtcacct
    14701 tgccgtggcc ttccacgcgc gacaccgggt cgatggcgac gcggcgcagt ttttcggggt
    14761 gctcggcagt ctcaaggttg ttcatggcgg ttctcaatcg taatgcatca gtgcgtgacc
    14821 cagctccggc tcgcgcccgg ccagcaggtc ggtcaggaac ctccagatgg cgtcgcccga
    14881 cggtgggcag ccgggcagga aataatccac ctttaccacc tcgtggatcg ggtgcacctt
    14941 gtcgagcggc agcggcaact ccgggtcgtt ggggatgccg ccgtgcgcca gtccggcttc
    15001 cgccaggtaa atctcgttga ggatcaccga tagcgggatg gggttgcgct gcgccggcag
    15061 gcccccatta atggcacagg cgccgaccgc caccaggatt ttgcaatgct ggcggaactc
    15121 acgcagcacg tgtacgtttt cggcattgca cagcccgccc tcgatcaggc cgatgtcgca
    15181 ctgcccgact tgcttgatat cggtaagcgg cgatcggtcg aactcgacca gttccaccag
    15241 atcgaacagg cgctcgtcga tgtccaggaa cgacatgtgg cagccgaagc agcccgccag
    15301 cgtggcggtg gccagcttaa gcttcttttt tacaggagca tccatgtcag tcctcatcgt
    15361 gcgtcagacg cgtatcgacg tacacatcga tcggctgcct gtcgaaagtg cgctcgccga
    15421 tgggcacggc gaagccccgc cgcttgggca ggatcacccc gaccgggcaa acctgcgccg
    15481 ccatgtcgag cgcgctgaaa tcggtgtcgg ccagttttcc ggatttggcg ttgacgatca
    15541 ggtgcgattg gatgccgcgc cccgccagcg cgaacacgtt cttgccatcc acgtcgcgac
    15601 tggcgcgaac gcacagcgaa cacaggatgc agcggttgaa gtcgagcagg aaatccgggt
    15661 gcgaagcatc gaccggacgg ttggggaaga agtggtcata atgcgcggtc gtcataccca
    15721 ggtggtaggc agtggcctgc aactggcagt cgccgctttt ctcgcacgac gggcagaaat
    15781 ggttgccctc gacgaatagc atctgggtca gtgccacccg ctccgcgttg agctcctcgg
    15841 tctcgctttc caccaccatg ccctccgtcg cctgcatggt gcaggaagcg gtctggcggc
    15901 cgttgaccgt gaccgtgcac agcttgcagg aaccgtgcgg cttgaattcc gggtggtggc
    15961 acaggtgcgg aatatagtgg ccagcgtcca gcgccgcttg gatgatggtc tggccgtctt
    16021 cgaacggcac atccttgccg tccagggtga aggtcttgct catgtgtttt cttccacatt
    16081 atgcagatgg gcaccactat cgtcgcgtcc ggtcatctgg cgcgccgcct gcaacgcata
    16141 gtcgaggtcg aaggcgggag tgaagtcgcg gtgcagcatg cgcgtttcat aggccgggcg
    16201 aaacttggcg atagtgtcca gcaccgggtt gcaggcggtg tggccgaggc cgcagtggct
    16261 catggacagc agcagcttat tcagcttctc gatttcctcc aggtcgtaag gcgagccgtg
    16321 gccatcatgc agcttgtcca tcaggttgcg caacagcgac gttcccacgc ggcagggcgt
    16381 gcaaaagccg cagctctcgt gggcgaagaa gtgagtgaag ttgcgcgcca cctcgaacat
    16441 gtcgcggctg gtgtcgaaca ccgtggaggc gcctggggtg ccgatgtccg cgaaaccgag
    16501 gatgcggtcg aactcctggc gcgacaccgt caccccggac ggcc
//
LOCUS       DAOMFA010000015        16211 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_278_length_16211_cov_5.092845, whole genome shotgun sequence.
ACCESSION   DAOMFA010000015 DAOMFA010000000
VERSION     DAOMFA010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 16211)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16211)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16211
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_278_length_16211_cov_5.092845"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..702
                     /locus_tag="PKW44_01565"
     CDS             <1..702
                     /locus_tag="PKW44_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HQR50307.1"
                     /translation="GEQVTTHWNMRAKLMAEMNEAQAKQGEYFTKVAAMSYEDLSKDA
                     ELMQFVNSAGKILFADNCAACHQAGGQGKLGLAPNLVDDSWIYGGTHEKVQETITGGR
                     NGVMPAFKGVLPDDKIVQVANYVLSLSGEPHDATAAKSGDAIFHNEGGCMVCHGAEGQ
                     GNPDLGSANLTDKIWLWADVPGQTTAEGKLQVVDALITGGMSRGKMPPWSGRLKPEQI
                     KLLTVYVHDSLGGGK"
     gene            806..2239
                     /gene="ccoG"
                     /locus_tag="PKW44_01570"
     CDS             806..2239
                     /gene="ccoG"
                     /locus_tag="PKW44_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HQR50308.1"
                     /translation="MTAADKASPLFQKHIPIFVRSVEGKFRNFKTGILVLAYSVFFAL
                     PWLPWERVSAANQAVLFDLGSRRFFIFNLVVYPQDIFWLSMLLFIAAALLFFVTGLVG
                     RAWCGYFCFQTLWADMFMLIEHMIQGERPARMRLYKQPWNMEKVLKVGSSHFFMILIS
                     FWTGFSFVAYFAYAPELLQNFFTGHAADAAYITVITLTITTYVAGGLSREQICLHICP
                     YARFQSVMYEPDTLAVSYDTRRGEGSTGRVLPMPGLRTREERQAMGHGDCIDCGFCVQ
                     VCPTGIDIRNGLQYQCISCGLCIDACNNIMESVGFPRGLIRYDSERNLHSAHPEKPQL
                     KWKRLKVLGYATALLIMIGALIYSVATRTNTELRVQQVRQPLFVELADESVRNRYQLH
                     IVNKTEHEETYKIEVRGIPATALDLGAVSEIRARAGKDLRLNAKVDLEEHLAEQTHEF
                     EFVVTPASDPEGQVVRKVSFSSKREKH"
     gene            2256..3026
                     /locus_tag="PKW44_01575"
     CDS             2256..3026
                     /locus_tag="PKW44_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HQR50309.1"
                     /translation="MTETLFGGLLAVILIFFVARRLGLSNYWSGVLGGSLPFLIYLGY
                     SANHGQGGDVIAIHLVVFMATAGVLGVFNNMQRGKEKMHWAPRLLIAFFALLAVAMAI
                     FVSIAANGLPPVIAKYVLPNANGRVMHTGFPGALPHDRNKLYEEHQQRIEAQRKLGWQ
                     VSVAGLEAMHSGVPFAISLTLQDAGGQPIIAEHVVLQMLRLAKHDDDFQLELKQTSPG
                     TYGTQVTLPASGNWVAGFLIERGQDTYEMQQPVQVADR"
     gene            3042..5456
                     /locus_tag="PKW44_01580"
     CDS             3042..5456
                     /locus_tag="PKW44_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HQR50310.1"
                     /translation="MSTDQPCYHCGLPVAEASPFQAEILGKRHSLCCHGCQAVAEAIV
                     ANGLEDYYRYRTELPRTAEELVPEELRQLDLYDHPEVQKSFVHQSAGNLKEASLILEG
                     ITCAACIWLNERHLRQLPGVHEVAVNYASHRARISWDDNKIKLSTILAEIRKLGYHAH
                     PFNAQQQEERRQVQRKLDIRRMAVAGISGGQVMMLAVALYAGTSQGMEFSTAQLLRWF
                     SLLLTAPAMLYSALPFYQSAWRAFKVRQVNMDTPISLGILAGFAGSVWATVQGQGVVY
                     YDTITMLIFFLLATRFLERNAREKSVEAAENLLRLVPAMATRVEEGQNVLVPVHDLND
                     GNLILAKPGETIAADGVVEQGESSADESLLTGESRPLPKKVGDRVFAGSINYESPLLI
                     RVTGVGEETVLAGIARLLDRAQSQKPRMAQAADRIAVWFTHALLVLVAVVGLVWWTIE
                     PPRAFEIMLAVLVVSCPCALSLAAPAAFAAAGSHLVKRGVLLTRGYALETLARATHFV
                     FDKTGTLTQGKPVLLRTIPVAQFPAEEYLRIAASLEQASEHPLAQSFLQAAGRQVLHV
                     VSDSGNVPGKGVAGIITGQRYTLGNVALRPELAGQCNLAGQELPTGASVVWLCDTQSV
                     LAAFVLADTPRPQAKQMVSLLQARGIRVSIFSGDDAEAVAHHARELGVDDWQAALSPE
                     RKLSEVSRLQQTGAVVAMVGDGINDAPVLAAAQVSIAMGSGTQMARTTGDIVLLTENL
                     LEIDHAVATSRFSMNVIHQNFTWALGYNLVVLPFAATGFISPWLAALGMSVSSLVVVL
                     NALRLR"
     gene            complement(5515..5850)
                     /locus_tag="PKW44_01585"
     CDS             complement(5515..5850)
                     /locus_tag="PKW44_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HQR50311.1"
                     /translation="MNFGDAIKTCFSKYADFSGRAKRPEFWWFVLFLFLVSLVLGLVS
                     NTVSLLFSLGTLIPSLAVGARRLHDTNRSGWWQLIGLIPVIGWIVLIVFCAQEGESGD
                     NQYGAPPGN"
     gene            complement(5924..6631)
                     /locus_tag="PKW44_01590"
     CDS             complement(5924..6631)
                     /locus_tag="PKW44_01590"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HQR50312.1"
                     /translation="MLSITIAGKPLPYTLKISARRRTLGLRVDQHGLTVHIPTRVPQH
                     TVESLLQDKADWIARKLAEWQERPGPASMEWQDGAALQHLGQEIQLVLRQDARNRAVE
                     FDGARLYVALPNPDDSKTVQRKVAQWYTRQARADFTRRIELLAARLGVPTPKLFLSNA
                     QTRWGSCNSKGEIRLHWRLIQAPPHIIHYVVAHELAHLKEMNHSPKFWAWVEKLCPDY
                     TSARNELKALSASLHLL"
     gene            complement(6654..7367)
                     /locus_tag="PKW44_01595"
     CDS             complement(6654..7367)
                     /locus_tag="PKW44_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HQR50313.1"
                     /translation="MIRLRSLIFNLGMWVMVIPYTLLALLLLPMSAPRRSRIIAGWAR
                     FVVRWLALTCNVHYRVIGQDNIPQHTCVILSKHQSAWETIAFQAIFPPQIWVLKRSLL
                     LLPFFGWALWALKSIAIDRSAGREALKQLVSQGKDRLARGLWVVVFPEGTRTAPGQKG
                     KYHIGGAWLATHAKATVVPVAHDAGEFWRKNSLLKHPGTITVSIGQPIDATGMKPDDL
                     NKQVEAWIEREMPRLKEYV"
     gene            complement(7364..7906)
                     /gene="gmhB"
                     /locus_tag="PKW44_01600"
     CDS             complement(7364..7906)
                     /gene="gmhB"
                     /locus_tag="PKW44_01600"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HQR50314.1"
                     /translation="MKLVILDRDGVINYDSAQFIKNPEEWIPIPGSLEAIALLNQAGF
                     RVALATNQSGISRGLFDMATLNAIHDKMHRTLAQFGGRIDALFYCPHTADAGCDCRKP
                     RAGMMEEIGRRFATDLEGVPSVGDSLRDLQASVAVGAQPILVLTGKGERTFAAGNFPE
                     NTWVCADLMEAAQRIIDEMA"
     gene            complement(7909..10038)
                     /gene="glyS"
                     /locus_tag="PKW44_01605"
     CDS             complement(7909..10038)
                     /gene="glyS"
                     /locus_tag="PKW44_01605"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HQR50315.1"
                     /translation="MSTKNLLVELFVEELPPKALKKLGEAFAVTLFESLKAQGLANDD
                     AEVTPYASPRRLAAHITQVAVQAADKAVSQKLMPAAVGLDANGQATPALLKKLAALGA
                     DESAVSQLKRENDGKADVLFLDAVQTGARLADGLQKALDEALARLPIPKVMTYQLDDG
                     WSSVNFVRPAHGLVALHGADVVPIHTLGLASSNLTQGHRFEAAKHPVTLKDADSYAAQ
                     LEHDGAVIARFAARRAEIARQLSEAAAREGLKPIDDDALLDEVTALVERPNVLLGQFE
                     AAFLEVPQECLILTMKANQKYFPLLDANGRLTNKFLLVSNIRPADPSAVIGGNERVVR
                     PRLADAKFFFDQDRKKTLESRVEQLDKVVYHNKLGSQGLRNERVKWIAATLGDRLGGE
                     ALARAAVQAASLAKADLLTDMVGEFPELQGVMGRYYAQHDGLGNDIAFAIEDHYRPRF
                     AGDELPRNPIGIVVALADKLETLTGLFGIGEKPSGDKDPFALRRHALGIIRMLVERNL
                     PLGLNELLDIGENCWLMGANNQPVHSLFADAPAQLAEFIYDRLAGNLRDQGYSVQEVD
                     AVLALRPQRLGDIAKRLAAVRAFAALSEAESLAAANKRVGNILKKAEGTVETKVDAAL
                     LKETAETELYQALATIKPQADAGFERGDYTVSLQALAALKAPVDAFFDNVMVNADDPA
                     LRANRLGLLATLHQAMNRVADLSRLAA"
     gene            complement(10035..10955)
                     /gene="glyQ"
                     /locus_tag="PKW44_01610"
     CDS             complement(10035..10955)
                     /gene="glyQ"
                     /locus_tag="PKW44_01610"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HQR50316.1"
                     /translation="MLTFQQIILTLQQYWDKQGCALLQPYDMEVGAGTSHTATFLRAL
                     GPEPWKAAYVQPSRRPKDGRYGENPNRLQHYYQYQVVLKPAPANILELYLGSLEALGF
                     DLNKNDIRFVEDDWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGINCKPITGEITY
                     GLERLAMYLQGVDNVFDLTWTDGLKYRDVYHQNEVEQSAYNFEYSDVEFLLGAFGSHE
                     KEAKLLMENQLALPAYEQVLKAAHTFNLLDARGAISVTERAAYIGRIRNLARSVAQSY
                     LDSRARLGFPMAPKAWSDEVLAQIEKKAAA"
     gene            complement(10992..12467)
                     /gene="lnt"
                     /locus_tag="PKW44_01615"
     CDS             complement(10992..12467)
                     /gene="lnt"
                     /locus_tag="PKW44_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HQR50317.1"
                     /translation="MPRLPSLSPSPRLAALLLGTLAVAGYAPFYLYPLSVLALAGLFR
                     LWRGDASPRQSFLLGYCYGLGLYGAGVSWIYVSLHDFGGMSLPLAALATAALCAFLAL
                     FPAAAGWLAAQFGSRRAWAMPAAWVLTEWVLNWIFTGFPWLTLGYSQVPHSPLAGLAP
                     LLGVFGVSLAAACCAALLAAWLDGQPRRPIIIALLAFWVTGSALKHIEWTQPQGAPIS
                     VSLLQGNIPQDLKWQAEAIESTLRTYLALARQHPAQLTVLPETAVPLLLHHVPANYLQ
                     ALAEPARQSGGDLLLGVAEQVGDNYYNSMVSLGTAASQNYRKVHLVPFGEFIPFKSAI
                     GWIYNDLLHIPLADLSPGKTDQRPLQVAGQRVAINICYEDVFGEEIIRQLPQATLLVN
                     ASNDAWYGRSSAAHQHLQISQTRALESGRMMLRATNTGATAIINPQGEVVAEAPHFTT
                     AALTGQAQGYSGVTPYVHWGNWPAISLVFLTLGVLWLRKKK"
     gene            complement(12516..13364)
                     /locus_tag="PKW44_01620"
     CDS             complement(12516..13364)
                     /locus_tag="PKW44_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HQR50318.1"
                     /translation="MAAASRLDSADHSRDSAGMTYVYPVVSRRAGGVSIGINLNPNNA
                     CNWRCIYCQVPGLTRGAAPAIDLPLLEQELRTMLDEVVNGDFLRQHVAEGARHLEDIA
                     FSGNGEPTSASEFPQAVALVENVLRDFGLLGRIKLRLITNGSLLDRPGVPEGVAHLAR
                     CNGEVWFKVDAATATGITRINDVHLKPEGVLQRLRGCAQRCPTWVQTCVFALDGRPPD
                     EVETRAYLDLLQQAAGDIAGVHLYSLARPSMQAEAPRLGRLPEAWLEALASRIRQLGL
                     TVRVSP"
     gene            complement(13373..13798)
                     /gene="fur"
                     /locus_tag="PKW44_01625"
     CDS             complement(13373..13798)
                     /gene="fur"
                     /locus_tag="PKW44_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HQR50319.1"
                     /translation="MKDPQDLKNAGLKATLPRLKVLGLFESSQQRHLSAEDVYRILLT
                     GGEDVGLATVYRVLTQFEQAGLLVRHHFESGKAVFELNQGGHHDHIVCLQCGRVEEFY
                     DEEIEARQKKAASERGFSIHDHSLHIYADCTKHPCPYKS"
     gene            13870..14682
                     /locus_tag="PKW44_01630"
     CDS             13870..14682
                     /locus_tag="PKW44_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HQR50320.1"
                     /translation="MRNATLLLALFCAACSSSLPSFTPYVMEIQQGNVVTPKMMMQLR
                     PGMSKAQVRFVMGTPLLADSFHANRWDYFYELRKQGKIVEQRNVVLEFENEKLKSVRG
                     DVIPSAAEIGKTPPAGAAPQGAPAIAPLPLPSFEKQTAPAPQAAPAAEPAPQSAPAEA
                     PKAEPKAAPVATPAPEPVKPVPVPVPAPKAEPAPQAAPTPKAAPAAAPVVAPAAAPAA
                     VPAPKAAPKPAAQPAPKPVAKPEPKPVPTPEDDLPPEEDPSYFERMLEKIGF"
     gene            14686..15486
                     /gene="dapB"
                     /locus_tag="PKW44_01635"
     CDS             14686..15486
                     /gene="dapB"
                     /locus_tag="PKW44_01635"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HQR50321.1"
                     /translation="MVNVVIAGSSGRMGHALLEAAVNDPELKLVAALDRADCPQLGKD
                     AGELFGAPCGVRISADTNAALQPGSVLVDFTRPEPSLVYLEACRKAGAGMVIGTTGFN
                     TAQKEAIAAAAKDIPIVFAPNMSVGVNLLINLVQAAAKVLAEGYDIEIIEAHHRHKVD
                     APSGTALRLGEAAAAALGRDLAQCAVYGREGVTGERDASTIGFATVRGGDVVGDHTVL
                     FAGIGERVELTHKASSRATFALGALRAAKFLASKKNGLFDMQDVLGLR"
     gene            15587..15811
                     /locus_tag="PKW44_01640"
     CDS             15587..15811
                     /locus_tag="PKW44_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018076952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HQR50322.1"
                     /translation="MQALKLTQIGNSVGLILPKEVLARLKLEKGDTVYLTDAPGAVTI
                     TPHDPTFEEQVDAGREFMREYRDTFNALAK"
     gene            15912..>16211
                     /locus_tag="PKW44_01645"
     CDS             15912..>16211
                     /locus_tag="PKW44_01645"
                     /inference="COORDINATES: protein motif:HMM:TIGR01550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HQR50323.1"
                     /translation="MLDSALARPVNLAAYGEPDFADLAACYGAGFAQNHPFVDGNKRA
                     AFLAVDLFLFLNGKRLTATQVDATVTMLALAAGELEEADLAAWLRQNSADKPNNSE"
BASE COUNT         2937 a         4871 c         5269 g         3134 t
ORIGIN      
        1 ggtgagcagg ttactacaca ctggaatatg cgtgccaagt taatggccga gatgaacgag
       61 gcacaggcca agcagggcga atacttcacg aaggtggccg ccatgtcgta tgaggatctt
      121 tccaaggacg ccgagttgat gcagttcgtc aattccgccg gaaagatcct gttcgccgac
      181 aactgtgccg cctgtcacca ggcggggggg cagggcaagc ttggattggc gcccaacctg
      241 gtggacgata gctggatcta cggtggtacg cacgagaaag tccaggaaac catcaccggc
      301 ggacgcaacg gtgtgatgcc tgcgttcaag ggcgtgttgc cggatgacaa gatcgttcag
      361 gtcgccaact atgtgctgag cctgtccggc gagccgcatg atgccacggc cgccaagtct
      421 ggcgatgcaa tcttccacaa cgaaggtggt tgcatggtct gccacggtgc cgaggggcag
      481 ggcaatcctg acctcggttc agccaacctg accgacaaga tctggctgtg ggccgacgtg
      541 cccggacaga ccactgccga aggcaagctg caggtggttg acgcccttat caccggcggc
      601 atgagccgtg gcaagatgcc gccatggagc ggacgcctca agcccgagca gatcaaattg
      661 ctgaccgtgt atgtgcacga tagcctcggt ggcggcaagt aagctatagt tataccgtca
      721 gtttgaacca gtacaaaggc tcgcgcttaa acgtgagcct ttgttctttt gaactttgtt
      781 ttttgtacat cgtcgtcagg taaaaatgac cgcagcagat aaagcatcgc cgcttttcca
      841 gaagcacatc ccgattttcg tgcgttccgt cgaagggaaa ttccgtaatt tcaagactgg
      901 cattctggtg ctggcctaca gcgtgttttt cgccttgccc tggctgccgt gggagcgtgt
      961 cagtgctgca aatcaggcgg tgttgtttga tctgggttca cggcggtttt tcattttcaa
     1021 cctggtagtg tatccgcagg atattttctg gctgtccatg ctgctgttca tcgctgccgc
     1081 cctgctgttt ttcgtgactg gcctggtagg gcgtgcctgg tgcggctatt tctgtttcca
     1141 gacgctgtgg gcggatatgt tcatgttgat cgagcatatg atccagggcg agcggccggc
     1201 cagaatgcgg ctctacaagc agccatggaa catggagaaa gtcctcaagg tcggatcatc
     1261 acatttcttc atgatcctga tttccttctg gaccgggttc agcttcgttg cctatttcgc
     1321 ctacgcaccg gagttgctgc aaaacttttt caccgggcat gcggcagacg cagcctatat
     1381 tacggtcatc accttgacca ttaccacgta tgtggcaggc gggctttcgc gcgagcagat
     1441 atgcctccat atctgtcctt acgcgcgttt ccagagcgtg atgtacgagc ccgacaccct
     1501 cgcggtcagc tacgatacgc ggcgcggcga aggctccacc ggccgcgttc tgcctatgcc
     1561 aggcctgcgc acccgcgaag aacgacaggc catggggcac ggcgactgta tcgactgcgg
     1621 tttctgcgtg caggtgtgcc cgaccggaat cgacattcgc aatggtttgc agtaccagtg
     1681 tatttcctgc ggcctgtgca tcgacgcctg caacaacatc atggagtcag tcggcttccc
     1741 gcgcggactg atccgctacg attcggaacg taatctgcac agcgcgcacc cggaaaagcc
     1801 tcagctcaaa tggaagcgcc tcaaagtgct tggctatgct accgcgctgc tcatcatgat
     1861 aggcgcgctg atatacagcg tcgccacccg caccaacacc gaactgcgcg tccagcaggt
     1921 acgccagccg ctattcgtgg agcttgccga cgaaagcgtg cgcaaccgct atcaactaca
     1981 tatcgtcaac aagaccgagc acgaggagac ctacaagata gaagtgcgcg gcatccccgc
     2041 caccgcgctc gatctgggtg cggtgagcga gattcgcgcg cgcgcgggca aggatctccg
     2101 actcaatgcc aaggttgacc tcgaagaaca tctggcagag cagacacatg agttcgaatt
     2161 cgtggtcacg ccagcatccg accccgaggg tcaggtggtg cgtaaagtca gcttcagcag
     2221 caaacgcgaa aaacactgaa cggaaactat aaccaatgac tgaaacccta ttcggcggct
     2281 tgctggccgt cattttaatt ttcttcgtgg cgcgtcgcct ggggctctcc aattactgga
     2341 gtggcgtact cggcggcagc ctgccttttc tgatctacct tggttacagt gcgaaccacg
     2401 ggcagggggg ggatgtcatc gccatccatc tggtggtatt catggcgacg gcgggagtgc
     2461 tgggtgtttt caacaacatg cagcgcggca aggagaaaat gcactgggcg ccacggttgc
     2521 tgattgcatt ttttgccctg ctggcggtcg ccatggcgat ttttgtgtcg attgccgcca
     2581 acggcttgcc tcccgtcatt gccaaatatg tgttgccgaa cgccaacggc cgtgtaatgc
     2641 ataccggctt ccccggcgca ctgccgcatg acagaaacaa gctgtacgaa gagcatcagc
     2701 aacggatcga agcgcagcgc aagctcggat ggcaggtgag cgtcgccggc ctggaggcca
     2761 tgcattctgg cgtacccttc gcgatcagcc tgacgctgca agatgccggc ggacaaccga
     2821 tcattgccga gcatgtggtg ttgcaaatgc tgcggcttgc caagcatgat gatgactttc
     2881 aactggagct gaaacagacc agccccggca cctatggcac gcaagtcact ctgccggcat
     2941 ccggtaactg ggtcgcaggc ttcctcatcg agcgtggtca ggatacttac gagatgcagc
     3001 agccggtgca ggtcgccgat cgttagcgac cgttttgctg tatgagcacg gatcagccct
     3061 gctaccactg cggcttgccg gtcgccgagg ccagcccttt ccaagccgag attctgggca
     3121 agcgtcacag cctgtgctgc catggctgcc aggcggtggc cgaagccatc gtcgccaacg
     3181 gcctggaaga ctactatcgt taccggactg aactgcccag gactgccgag gaattggtgc
     3241 cggaggaact gcgccagctc gacttgtatg atcacccgga ggtgcagaag agcttcgtgc
     3301 accagtccgc cggaaacctc aaggaagcct cgctgattct cgaaggcatc acctgcgccg
     3361 cctgcatctg gcttaacgaa cgccatttga ggcagctgcc cggggtgcat gaagtcgctg
     3421 tcaattacgc ctcccatcgt gcccgcatca gttgggacga taacaagatc aagctttcca
     3481 ccatccttgc cgaaatccgc aagcttggct accacgccca cccgttcaac gcccagcagc
     3541 aggaagaacg ccgccaggtc cagcgcaagc tcgacattcg ccgcatggcg gtggccggga
     3601 tttccggcgg ccaggtgatg atgctggcgg tggcgctata tgccggcacc tcgcaaggca
     3661 tggaattctc caccgcgcag ctgctgcgct ggttcagcct gctgctgacc gcgcccgcca
     3721 tgctttattc cgcgctgccg ttctaccagt cggcctggcg cgcgttcaaa gtacgccaag
     3781 tcaacatgga cacacccatc tcgctcggca tcctggccgg attcgccggc agcgtatggg
     3841 ccaccgtcca ggggcaaggc gtggtgtatt acgacactat caccatgttg attttcttcc
     3901 tgctggcgac gcgcttcctg gaacgcaacg cccgcgagaa gtcggtggaa gcggcggaaa
     3961 acctgctgcg cctggtgccg gccatggcaa cccgtgtcga agaaggtcaa aacgtgctgg
     4021 tgccggtaca tgacctcaat gacggcaacc tgatccttgc caagccgggc gaaaccatcg
     4081 ctgccgatgg cgtggtggaa cagggtgaaa gcagcgccga cgagtccctg ctcaccggcg
     4141 agagccggcc gttgccgaag aaggtgggcg accgcgtgtt cgccggcagc atcaattacg
     4201 aaagtccgct gctgatccgc gttaccggcg tgggcgaaga aaccgtgctg gccggcatcg
     4261 cgcgactgct ggatcgcgcg cagtcgcaaa agccccgcat ggcccaggcg gcagaccgta
     4321 tcgcggtctg gttcactcat gcgctgctgg tgctggtggc ggtggtcggc ctagtctggt
     4381 ggactatcga gccgccacgc gcgttcgaga tcatgctggc ggtgctggtg gtgagctgcc
     4441 cttgtgcgct gtctttagcc gctccagccg ccttcgccgc cgccggctcg catctggtca
     4501 agcgcggcgt gctgctcacg cgcggctacg cactggaaac gctggcgcgg gcgacccact
     4561 ttgtcttcga caagaccggc acgctcaccc agggcaagcc ggtgctgttg cgcaccatcc
     4621 ccgttgcaca atttccggcg gaagaatacc tgcgcatcgc cgccagcctg gaacaggctt
     4681 cggagcaccc gctggcgcaa agttttctgc aggccgcagg gcggcaagtg ctgcatgtgg
     4741 tcagcgatag cggcaacgtg ccgggcaagg gtgtggccgg aatcatcacc gggcagcggt
     4801 ataccctggg caatgtcgcc ctgcggccgg aactggccgg ccagtgcaat ctggccgggc
     4861 aggagctacc caccggcgcc agcgtagtct ggctgtgcga tacgcaatcc gtactggccg
     4921 ctttcgtact ggccgacacc ccgcgtccgc aggccaaaca gatggtttcg ctcctgcagg
     4981 cgcgcggcat ccgtgtctcc atcttcagtg gtgacgatgc cgaggcggtc gcccaccatg
     5041 cgcgcgaact cggggtggac gactggcagg ccgccttgtc gccggaacgg aagctctccg
     5101 aggtcagccg tttgcagcaa accggtgccg tggtcgccat ggtcggcgat ggcatcaacg
     5161 atgccccggt actggctgcc gcgcaggtgt ccattgccat gggcagcggt acgcagatgg
     5221 cgcgcaccac cggagacatc gtgctgctga cggagaacct gctggaaatc gaccacgccg
     5281 ttgcaaccag ccgtttcagc atgaacgtga ttcaccagaa tttcacttgg gcgctgggct
     5341 ataacctggt ggtgttgcca ttcgccgcga cagggtttat ttctccctgg ctggccgcgt
     5401 tggggatgtc ggtaagttcg ctggtggtgg tgctcaacgc gttgcggttg cgctgacggt
     5461 caagtgcaaa aagccggcac atgccggctt ttttgtgcga gcgatctgtc gcgatcagtt
     5521 gccgggggga gcgccgtact ggttgtcccc ggattcccct tcctgtgcgc agaacacgat
     5581 cagcacgatc cagccgatca cggggatcag cccgatcagc tgccaccagc cgctgcgatt
     5641 agtgtcatgc aggcggcggg caccgaccgc gagcgaggga atcagcgtcc ccaacgagaa
     5701 cagcaacgaa acggtattgc ttaccagccc cagtaccagg ctgaccaaga acaggaacag
     5761 cacgaaccac cagaattcag gccgtttggc acggccgctg aagtccgcgt atttggagaa
     5821 gcaggtcttg atggcgtcac cgaaattcat tattttttcc tttgttgttg agggaggtga
     5881 gtgagcagca gggaaaatct tagcatgctc gcagggttga gacctagagc agatgtagtg
     5941 acgccgacaa agccttcaat tcatttctgg cggaagtgta atccgggcac agtttctcca
     6001 cccacgccca gaatttcggg ctgtggttca tctccttcag atgcgcgagt tcgtgggcga
     6061 cgacgtagtg gatgatgtgg ggcggggcct ggatgaggcg ccagtgcagg cggatttcgc
     6121 cttttgagtt gcagcttccc cagcgggttt gcgcgttgga gaggaacagt ttgggggtgg
     6181 ggacgccgag tctggcggcg aggagttcga tgcggcgggt aaagtcggcg cgtgcctgtc
     6241 tggtgtacca ctgggcgacc ttgcgctgca cggttttgct gtcgtcgggg ttgggcaggg
     6301 cgacatagag ccgggcgccg tcgaattcca cggcgcggtt tctggcgtcc tgccggagca
     6361 cgagttggat ctcctgcccg aggtgttgca gcgcagcgcc gtcctgccat tccatggaag
     6421 ccgggccggg ccgttcctgc cattcggcga gtttgcgggc gatccagtcg gccttgtctt
     6481 gcagcaggct ttcgacggtg tgctgcggga cgcgtgtcgg gatatgcacc gtcaggccat
     6541 gctggtcgac gcgcaggccc agtgtgcggc gacgggcgct gatcttgagg gtgtaaggga
     6601 ggggcttgcc ggcgattgtg atgctgagca agcgcgggga atgcggcgat gccctagaca
     6661 tattccttca gacgcggcat ttccctttcg atccatgcct cgacctgctt gttcaggtcg
     6721 tccggtttca tgccggtggc atcgatgggt tggccgatgc tgacggtgat ggtgccgggg
     6781 tgcttgagca ggctgttctt gcgccagaat tcgccggcat cgtgggccac cggcaccact
     6841 gtcgccttgg cgtgggtggc gagccaggcg ccgccgatgt ggtactttcc cttctgcccc
     6901 ggcgcggtgc gcgtgccttc cgggaatact accacccaca ggccgcgtgc gagccgatcc
     6961 ttgccttgcg acaccagttg cttcaaggcc tcgcgcccgg cggagcggtc gatggcgatg
     7021 gatttgagcg cccacaacgc ccagccgaaa aacggcagca gcagcagtga gcgtttcagc
     7081 acccagatct gcggcgggaa gatggcctga aaggcgatgg tttcccaggc cgactggtgc
     7141 ttggagagga tgacgcaggt gtgttgaggg atgttgtcct ggccgatgac gcggtagtgc
     7201 acgttgcatg tcagcgccag ccagcgcacc acgaagcgcg cccagccggc gatgatgcgg
     7261 gagcgccgcg gcgcggacat cgggagcagc agcagggcga gcagggtgta ggggatcacc
     7321 atgacccaca tgccgaggtt gaagatgagc gagcgcagcc ggatcatgcc atctcgtcga
     7381 taatgcgctg cgccgcttcc atcagatcgg cgcaaaccca ggtgttttcc ggaaaattgc
     7441 cggccgcgaa ggttctctcg cccttgccgg tgagcaccag gatgggctgg gcgccgacgg
     7501 cgacgctggc ctgcaggtcg cgcagggaat cgcccacgct gggaacgcct tccaggtcgg
     7561 tggcgaagcg gcggccgatt tcctccatca tgccggccct gggcttgcgg cagtcacagc
     7621 cggcatcggc ggtatgcggg cagtagaaca gcgcgtcgat gcgcccaccg aactgcgcca
     7681 gcgtacggtg catcttgtcg tggatggcat tgagggtggc catgtcgaat aggccgcggc
     7741 tgatgccgga ctggttggtg gccagcgcca cgcgaaagcc ggcctggttc agcagcgcga
     7801 tggcttccag gctgccgggg atgggaatcc actcctccgg gttcttgatg aactgggcgc
     7861 tgtcgtagtt gatgacgccg tcgcggtcga ggatgacgag tttcacggtt atgctgccag
     7921 cctggaaaga tcggccacgc ggttcatggc ctggtgcaac gtcgccagca ggccgagacg
     7981 gttggcgcgc agggcggggt cgtcggcgtt gaccatgacg ttgtcgaaga aggcgtccac
     8041 cggggctttc agggcagcca gcgcctgcag cgaaacggtg tagtcgccgc gctcgaagcc
     8101 ggcatcggct tgcggcttga tggtggctag agcctggtat agctccgttt ccgctgtctc
     8161 cttgagcagg gcggcgtcta ccttggtttc caccgtgcct tcggccttct tcaggatatt
     8221 gccgacccgc ttgttggcag ctgccaggct ttcggcttcg gacagcgcgg cgaaggcgcg
     8281 cacggcggct aaacgcttgg cgatatcgcc caagcgttgc gggcggagcg ccagcaccgc
     8341 atcgacttcc tgtacgctgt aaccctgatc gcgtaggttg ccggcaaggc ggtcatagat
     8401 aaactcggca agttgggcgg gcgcatctgc gaaaagcgag tggacaggct ggttgttggc
     8461 ccccatcagc cagcagttct cgccaatatc caatagctcg ttaagtccaa gtggcaggtt
     8521 tcgctcaact agcatacgaa tgatgccaag tgcatgacga cgcaaggcaa acggatcttt
     8581 atcaccacta ggtttctcgc caatgccgaa caggcctgta agtgtttcca gcttgtctgc
     8641 cagcgccacg acgatgccga tcgggttgcg cggcaattcg tcgcctgcaa acctaggccg
     8701 gtaatgatct tcgatggcga aggcgatatc gttaccaagg ccgtcgtgtt gcgcgtagta
     8761 gcgccccata acaccttgca gttccgggaa ttcgcccacc atgtcagtca gtaggtcagc
     8821 ttttgcaaga ctcgcagcct gtaccgctgc tctggcaagg gcctcgcctc ctagcctatc
     8881 gccgagcgtc gcagcaatcc atttgacgcg ttcgttgcgc agcccttgcg agccgagctt
     8941 gttgtgataa accaccttat ctagttgttc tacgcgcgat tccagcgtct tcttgcggtc
     9001 ctggtcgaag aagaacttgg cgtcggccag gcgcgggcga acgactcttt cattgccacc
     9061 gatgacggcg ctggggtcgg ccgggcggat gttggacacc agcaggaact tgttggtgag
     9121 gcggccgttg gcatccagca gcgggaagta tttctggttg gccttcatgg tgaggatcag
     9181 gcactcctgc ggtacctcca ggaaggccgc ctcaaactgg ccgagcagca cgttggggcg
     9241 ctccaccagc gcggtgactt cgtccagcag ggcatcgtcg tcgatgggtt tcaggccttc
     9301 cctggcggct gcctccgaca gttggcgggc gatctcggcg cgccgcgccg cgaatctggc
     9361 gatgacggcg ccgtcatgtt ccagttgcgc ggcatagctg tcggcatcct tgagcgtgac
     9421 cgggtgcttg gccgcctcga agcggtggcc ctgggtcagg ttgctggagg ccagtcccaa
     9481 cgtatggata ggcaccacgt ccgcgccgtg caatgcgacc agcccatgcg ccgggcgtac
     9541 gaagttgacc gaactccagc cgtcgtccag ttggtaagtc atgaccttgg gaatgggcag
     9601 tctggccagc gcttcatcca gcgccttttg cagaccgtcg gccaatctgg cgccggtttg
     9661 caccgcatcc aggaacagca cgtcggcctt gccgtcgttt tcgcgcttga gctgggatac
     9721 ggcgctttcg tccgcgccca gcgcggccag tttcttcagc agggcagggg tggcctggcc
     9781 gttggcatcc aggccgacgg cggccggcat cagcttctgc gataccgcct tgtcggcggc
     9841 ttgcacggcg acctgggtga tgtgtgcggc aaggcggcgt ggcgaggcgt aaggggtaac
     9901 ctcggcatcg tcgttagcca ggccttgcgc cttgagcgat tcgaacaggg tgacggcaaa
     9961 cgcctcgccc agctttttca gcgccttggg tggtagttcc tcgacgaaca attcgaccaa
    10021 caggtttttg gtgctcatgc tgctgctttc ttttcgatct gtgccagcac ttcgtccgac
    10081 cacgccttgg gcgccatcgg aaagccaagc cgtgcgcggc tgtccaggta gctctgtgcc
    10141 acgctgcgcg ccagattgcg gatgcggcca atgtaggcgg cacgctcggt gacggagatg
    10201 gcgccccgcg catccagcag gttgaaggtg tgcgcggcct tgagcacctg ctcgtaggcg
    10261 ggcagcgcca gctggttttc catgagaagt tttgcttcct tctcatggct gccgaaggcg
    10321 ccgagcagga attcgacgtc gctgtattcg aagttgtagg cggactgctc gacttcattc
    10381 tggtgataca catcgcggta tttgaggccg tccgtccaag tgaggtcgaa cacgttgtcc
    10441 acgccctgca ggtacatggc caggcgctcc aggccatagg tgatctcgcc ggtgatgggc
    10501 ttgcaattga tgccgccgac ctgctgaaag taggtgaact gcgtcacttc catgccgttc
    10561 agccacactt cccagcccag tccccaggcg cccagcgtgg ggttttccca gtcgtcctcg
    10621 acgaagcgga tgtcgttctt gttgaggtcg aagcccagcg cttccagtga gccgagataa
    10681 agctccagga tgttggccgg tgccggcttc agcacgacct ggtactggta gtagtgttgc
    10741 aggcggttgg gattctcgcc gtagcgtccg tccttggggc ggcggctggg ttgtacgtag
    10801 gccgccttcc acggctccgg gccgagggcg cgcaggaagg tggcagtgtg gctggtgccc
    10861 gcgccgactt ccatgtcgta cggctgcagc agcgcacagc cctgtttgtc ccagtattgc
    10921 tggagcgtca gaatgatttg ctggaaagtg agcattgaat aagattcagc aggttgcaaa
    10981 agggcctgat tttacttctt tttccggagc cacaaaaccc cgagtgtgag gaaaaccagg
    11041 gagatcgcgg gccagtttcc ccagtgcaca taaggcgtca cgccgctata gccttgcgcc
    11101 tgcccggtga gggcggcggt ggtgaaatgc ggcgcctcgg cgacgacttc gccttgcgga
    11161 ttgatgatcg cagtggcgcc ggtgttggtg gcgcgcagca tcatgcggcc gctttccagg
    11221 gcgcgcgtct gcgagatttg cagatgctgg tgcgccgccg aggagcggcc ataccaggca
    11281 tcgttgctgg cgttgaccag cagggtcgcc tgcggcagct ggcggatgat ctcctcgccg
    11341 aacacgtcct cgtagcagat gttgatcgcc acgcgctgtc cggcgacctg cagcggtcgc
    11401 tggtcggttt ttccggggga gaggtcggcc agcgggatgt gcagcaggtc gttatagatc
    11461 cagccgatgg ctgatttgaa cgggatgaat tcgccgaacg gcaccaggtg gaccttgcgg
    11521 taattctggc tggcggcagt gcccaggctg accatgctgt tgtagtagtt gtcgcccacc
    11581 tgttctgcca cgcccagcag caggtcgccg ccggattgcc tggctggctc ggccagcgct
    11641 tgcaggtagt tggccgggac atggtgcagc agcagcggca cggcggtttc cggcagcacg
    11701 gtgagttgcg ccggatgctg gcgggcgagg gcgaggtagg tgcgcagggt gctttcgatg
    11761 gcctcggcct gccacttgag gtcctgcggg atgttgccct gcagcaggct gacgctgatc
    11821 ggcgcgccct gcggctgggt ccattcgatg tgcttgagcg cgctacccgt cacccagaac
    11881 gccagcaagg cgatgatgat ggggcggcgc ggttggccgt ccagccacgc cgccagcaac
    11941 gcggcgcagc aagctgcggc cagcgaaacg ccgaacacgc cgagcagcgg ggcgagcccg
    12001 gccagcgggc tgtgcggcac ctgcgaatag cccagcgtca gccacgggaa gccggtgaaa
    12061 atccagttga gcacccattc cgtcagcacc caggcggcgg gcatggccca ggcgcgacgg
    12121 ctgccgaact gcgcggccag ccagccggcg gcggccggga acaacgccag gaaggcgcat
    12181 aaggcggcag tggcaagcgc cgccagcggc agcgacatgc cgccgaaatc gtgcaggctg
    12241 acatagatcc acgacacgcc ggcgccgtac agccccagcc cgtagcaata gcccagcagg
    12301 aaactttgcc tgggcgaggc gtcgccgcgc cacaggcgga acagcccggc cagcgccagc
    12361 acggagagcg ggtacaagta gaacggcgcg tagccggcga cggcgagagt gcccagcagc
    12421 aaggccgcga ggcgtgggct gggggaaagg ctagggagtc tgggcaaaat ggatcggttg
    12481 aaagaaatga attccgcgca ttatatccgg gctggtcaag ggctgacgcg tacggtcagg
    12541 ccaagttgcc ggatgcggct cgccaatgct tccagccatg cctcgggaag ccgccccagc
    12601 cgtggggctt cggcctgcat cgaggggcgc gccaggctgt agagatggac gccggcgatg
    12661 tcgcccgcgg cctgttgcag caagtccaga taggcgcggg tttccacctc gtcgggcggg
    12721 cggccgtcga gcgcgaacac gcaggtctgc acccaggtcg ggcagcgttg cgcgcaaccg
    12781 cgcaggcgtt gcagcacgcc ttccggcttg aggtgaacat cgttgatgcg tgtgatgccg
    12841 gtggcggttg ccgcgtcgac cttgaaccat acttccccgt tgcagcgcgc caggtgggcg
    12901 acgccttccg gcacgccggg ccggtcgagc aggctgccgt tggtgatcag ccgcagcttg
    12961 atcctgccca gcaaaccgaa gtcgcgcagt acgttttcga ccagcgccac ggcctggggg
    13021 aactcgcttg cgctggtcgg ctcgccgttg ccggaaaagg cgatgtcttc cagatggcgc
    13081 gcgccttccg ccacatgctg ccgcaggaaa tcgccgttca ccacttcgtc gagcatggtg
    13141 cgcagctcct gctccagcaa ggggaggtca atcgccggcg ccgcgccgcg cgtcaacccg
    13201 ggcacctggc aatagatgca gcgccagttg caggcgttat tggggttgag gttgatgccg
    13261 atggaaacgc cgccggctcg gcgggagact accgggtaga cgtaggtcat gccggcgctg
    13321 tcgcggctgt ggtcggcgct atcgagacgg gaagcggcgg ccacgccggt agtcagcttt
    13381 tgtaagggca agggtgcttg gtgcaatccg cgtagatatg cagggaatgg tcgtggatgc
    13441 tgaagccgcg ctcgctggcg gcttttttct ggcgcgcctc gatttcctcg tcgtagaact
    13501 cttccacgcg cccgcactgc aggcagacga tatggtcgtg gtggccgccc tggttgagtt
    13561 cgaacacggc cttgccgctt tcgaagtgat ggcgcaccag caacccggcc tgctcgaact
    13621 gggtgagcac gcgatagacg gtggccaggc cgacatcctc gccgccggtc aacagtatgc
    13681 ggtagacatc ctccgcgctg agatggcgct gctggctgct ttcgaacagg cccagcacct
    13741 tgaggcgagg cagggtggcc ttgaggccgg cgttcttgag gtcttgaggg tctttcatga
    13801 tgtgtcgggc caacgttatg tcgtggtata ttagcgccat tttccgccaa agtcataaag
    13861 tcataagcga tgcgtaacgc gaccctgttg ttggcacttt tctgcgctgc ctgttcctcc
    13921 agcctgcctt ccttcacccc ctatgtcatg gaaatccagc agggcaacgt cgtcaccccc
    13981 aagatgatga tgcagttgcg tccgggcatg agcaaggcgc aggtgcgctt cgtcatgggc
    14041 acgccgttgc tggccgacag cttccacgcc aaccgctggg attacttcta cgagttgcgc
    14101 aagcagggca agattgtcga gcagcgtaac gtggtgctgg aattcgagaa cgaaaagctc
    14161 aagagcgtgc gtggtgacgt gatcccgtca gccgccgaga tcggcaagac gccgccggcg
    14221 ggggctgcgc cgcagggcgc accggcgatc gccccattgc cgctgccgag cttcgagaag
    14281 caaaccgcgc cggcgccgca ggcagcacct gccgccgagc cggcaccgca atcggcgcca
    14341 gcagaggcgc caaaggccga acccaaggcg gcgcctgtcg ccacgcccgc gccggagcca
    14401 gtcaaaccgg tgccggtgcc agtccccgcg cccaaggccg aaccggctcc acaggcggct
    14461 cccacgccca aggccgcgcc tgccgctgcc cctgttgttg cgcccgccgc cgcaccggct
    14521 gccgtgcctg cgcccaaggc ggcgccgaag ccagcggccc agcctgcgcc caagcccgta
    14581 gccaagccgg aaccgaagcc cgtgcccacg ccggaggacg acctgccacc ggaagaagac
    14641 ccgagctatt ttgaacgcat gttagagaag ataggtttct aagagatggt aaatgtcgtg
    14701 attgccggga gttccggtcg catgggccac gcactgctgg aagcggccgt caacgacccc
    14761 gagttgaagc ttgtggccgc gctcgaccgt gccgactgcc cgcaactggg caaggatgcc
    14821 ggcgaactgt tcggcgcgcc ctgcggcgtc aggatcagcg ccgacacaaa cgcagccttg
    14881 cagccgggca gcgtgctggt ggatttcacc cggcccgagc ccagcctggt ttatctggaa
    14941 gcctgccgca aggccggcgc cggcatggtg atcgggacga ccgggttcaa tacagcgcag
    15001 aaggaagcca tcgccgctgc tgcgaaggac atccccatcg tcttcgcccc caacatgagt
    15061 gtcggcgtca acctgctcat caacctggtg caggccgccg ccaaggtgct ggccgaaggc
    15121 tacgacatcg agatcatcga ggcgcatcat cgccataaag tggatgcccc ctccggcacc
    15181 gccctgcgcc tgggcgaggc cgccgctgcg gcgctggggc gcgatctggc gcaatgcgcg
    15241 gtgtacggcc gtgaaggcgt caccggcgag cgcgatgcca gcaccatcgg cttcgccacc
    15301 gtgcgcggcg gcgatgtggt gggcgaccac accgtgctgt tcgccggcat cggcgagcgc
    15361 gtggaactga cccacaaggc cagcagccgc gccacctttg cgctgggcgc gctgcgcgcc
    15421 gccaagtttc tcgccagcaa gaaaaacggt ttgtttgata tgcaggacgt gctcggtctg
    15481 cggtagtgag cttgcccctg taacccagac cccaattatt ccgccttccc ataaaaattg
    15541 cgccagttga atttgcttga tgttataaca tcatgcggag ccagccatgc aagccctgaa
    15601 actcacccag atcggtaatt ccgtcggttt gatcctgccc aaggaggtgc tggcgcgcct
    15661 gaagctggag aagggtgaca ccgtctatct gaccgatgcg ccgggcgccg tgaccatcac
    15721 gccgcatgac cccacgttcg aagagcaggt cgacgccggg cgcgaattca tgcgcgaata
    15781 ccgcgatacc ttcaacgccc tggccaaata ggctggctgg atgtggacgt ggataaataa
    15841 tccgcgctgc tggtgctgca cgatgaaagc cttgcctgca tggtggcgct tcgggtgtcc
    15901 gcgaccaagg tttgctggat tcggcgctgg cgcggccggt caacctggcg gcctatggcg
    15961 agccggattt cgccgacctg gcggcctgct atggggccgg ttttgcccag aaccacccgt
    16021 ttgtggatgg caacaagcgt gcggcgtttc tggcggtgga cctgtttctg tttttgaacg
    16081 gcaagcgcct gaccgcaacg caggtggacg cgactgtcac catgctcgcg ctggccgccg
    16141 gcgagctgga agaagccgat ttggccgctt ggttacggca aaacagtgcg gacaaaccga
    16201 ataatagcga a
//
LOCUS       DAOMFA010000016        15934 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_288_length_15934_cov_5.636816, whole genome shotgun sequence.
ACCESSION   DAOMFA010000016 DAOMFA010000000
VERSION     DAOMFA010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 15934)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15934)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15934
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_288_length_15934_cov_5.636816"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(107..1831)
                     /gene="ptsP"
                     /locus_tag="PKW44_01650"
     CDS             complement(107..1831)
                     /gene="ptsP"
                     /locus_tag="PKW44_01650"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HQR50324.1"
                     /translation="MSFTMHGVAVSSGIAIGQAHLVSHALLEVAHYQVPRHLIEAEVV
                     RFGEAVDTVRYDLEGIRASLPANAPAELGSFVGTHIMILADKALSEAPKQIIRDEMCN
                     AEWALKQQMDDLVGQFEEIEDDYLRERKFDVVQVVERVIKVLLGHPSQAPLERQESGM
                     ILVAHDLSPADAIQFKQHQFAAFITDVGGATSHTAILARSLNIPSILALHHARDLIHD
                     GELIIVDGNQGVVIVNPDKNTLTEYRLKQEHWEIEQQKLKRLKLTRAVTLDGTPIELH
                     ANIEIPEDVAQTRAAGATGIGLYRTEFLFMNRREWPDEEEQFEAYRKVAEAMKGLPVT
                     IRTLDLGADKPANPDEVRTCANPALGLRAIRLCLAEPMIFHTQLRAVLRASHYGNVKI
                     LIPMLSSLSELRQSLHLLERAKRSLREESIPFDEAVQVGGMIEIPAAAIKAEAFAREL
                     DFLSIGTNDLIQYTLAIDRTDDSVAHLYNPLHPAVLQLIEMTIRAADKVGIPVSVCGE
                     TAGDPRLTRLLLGFGLRQFSMHPAHVLAVKHQVLQSDIPKLAATVRKILSSAEIDRIE
                     PLLLKLNQ"
     gene            complement(1865..2134)
                     /locus_tag="PKW44_01655"
     CDS             complement(1865..2134)
                     /locus_tag="PKW44_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HQR50325.1"
                     /translation="MVNLEIEIINKLGLHARASTKLTQVASRFGSEIWIARNGRRVNA
                     KSIMGVMMLAASKGSKVTLEASGPDEAAALQALAALINDKFGEGE"
     gene            complement(2127..2534)
                     /locus_tag="PKW44_01660"
     CDS             complement(2127..2534)
                     /locus_tag="PKW44_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HQR50326.1"
                     /translation="MIGILIIAHGSLGESLVHCASHVMGGEPPQLKQIGVGMHGDPAA
                     LLPMAREMVKSLDQGSGVLVLSDIYGATPCNLASQLLTPGRVEGISGVNLPMLVRALS
                     YRNEPLEVLVEKAIGGGVGGVIHFTQEKCLPHG"
     gene            complement(2560..3396)
                     /gene="rapZ"
                     /locus_tag="PKW44_01665"
     CDS             complement(2560..3396)
                     /gene="rapZ"
                     /locus_tag="PKW44_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HQR50327.1"
                     /translation="MQLVVVTGLSGSGKSVVLKALEDGGYYCVDNLPAKLLPETMRHL
                     EQAEQARVAVSIDTRSSTLETLPDIIGSLRAQGLEVQVLFLESSAETLVKRFSETRRR
                     HPLSGDNTTLAESIHQEREMLARLGDLGHRIDTSDLGPNTLRAWVKDFMALDHSGLTL
                     LFASFGFKHGIPLDADCVFDVRCLPNPHYDPVLRPQTGRDQAVIAFLQAQESVQAMAD
                     DIRQFVGNWLPCFVRDNRSYLTVALGCTGGQHRSVYLAEQLAAYFRTQGQRVLVRHRE
                     LE"
     gene            complement(3396..4361)
                     /gene="hprK"
                     /locus_tag="PKW44_01670"
     CDS             complement(3396..4361)
                     /gene="hprK"
                     /locus_tag="PKW44_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HQR50328.1"
                     /translation="MAKIDVAELYRDTRRNLKLKWVAGLEGGDNHLTSDTVTKPSLAL
                     IGHLNFVHPNRVQVLGCAEMDYLRSLDVPDMKRAIANLFSTDLAAVVVANGEVPPKAL
                     LDAAEKCKTPLFTSPVQSPRLMDVLSHYLAQAVAESASCHGVFMEVQGFGVLIKGDSA
                     IGKSELALELITRGHRLVADDVVDFYRISPERLEGRCPPLLQDFLEVRGLGILNVREL
                     FGDNAVKPTKPLDLIVQLELAETLAPQMLDRLKIKAQSENILEVKVPKVLIPVAAGRN
                     IAVLVEVAVRNHILLMRGINSTRQFTQRQNREIRRNARNIEKGGD"
     gene            complement(4424..4750)
                     /gene="raiA"
                     /locus_tag="PKW44_01675"
     CDS             complement(4424..4750)
                     /gene="raiA"
                     /locus_tag="PKW44_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019896968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HQR50329.1"
                     /translation="MNLHLTGHHLEITPALRDYVQAKLERVTNHFDQVIDVKVTLSVE
                     KLKQKVEATLHVPGNDMHAECSDDNMYSAIDLLSDKLNRQVLKHKEKQGDHHRSNGGI
                     KHQTAE"
     gene            complement(4780..6219)
                     /locus_tag="PKW44_01680"
     CDS             complement(4780..6219)
                     /locus_tag="PKW44_01680"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HQR50330.1"
                     /translation="MKQGLQLRLSQHLALTPQLQQSIRLLQMSTVELNQELESILQAN
                     PLLERVESSEGEGFVPAPAAAQEIATPQEESAKPEEYAADEFGTDYSDYSGGNRWDDS
                     GSGSDDEGDFVFQEADTPTLRQHLLGQIKLMPLSQRDLTLAALLVDAINEDGYLEQPL
                     EELASILPPEYEVDPLELATALKHIQHLDPPGIGARDLAECLSLQLQVLPPDTPQLAL
                     ASELVKSHLPALAVRDYAKLRKVLHCDEAALREAQSLIMQLNPRPAAGFTSLSSDHYI
                     QHEIVVKKVKGVWVASLNQEVMPTLRINQLYADILKRNRESSGQYLMSQMQEARWMIK
                     NIQQRFSTILRVSQAIVDRQRNFFEHGEIAMRPLVLREIADELELHESTISRVTTRKY
                     MLTPRGVYELKYFFGSHVSTDSGGACSATAIRALIKQLVAGETPKKPLSDSQIADILG
                     KQGIVVARRTIAKYRESLQIPAANQRKSL"
     gene            complement(6232..6954)
                     /gene="lptB"
                     /locus_tag="PKW44_01685"
     CDS             complement(6232..6954)
                     /gene="lptB"
                     /locus_tag="PKW44_01685"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HQR50331.1"
                     /translation="MSELIVENLRKRYKARTVVQDISLSVKSGEVVGLLGPNGAGKTT
                     SFYMMVGLVPLDGGRILLDGRDLGKLPIHRRARLGLSYLPQEPSVFRKLSVEQNILAI
                     LELRKLPLDQMEQELEELLQELHITHLRRNSAVSLSGGERRRVEIARCLATNPQFILL
                     DEPFAGIDPIAVLDIQKVIRFLTGRGLGVLITDHNVRETLGICDHAYIVNDGKVLAAG
                     HPDEIVHNESVREVYLGKDFRL"
     gene            complement(6965..7537)
                     /gene="lptA"
                     /locus_tag="PKW44_01690"
     CDS             complement(6965..7537)
                     /gene="lptA"
                     /locus_tag="PKW44_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020183540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0001530 - lipopolysaccharide binding
                     [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HQR50332.1"
                     /translation="MKIPRLNLSSHLLLLTALLFPGSALAEKADREQPIDLEADTVQV
                     NDAKKTSTYTGNVILTQGTLVIHADKLVVREDSLGFQHSTSTGNPTTFKQKLEGKNEY
                     MEGSAQRIEYDGRMDKVQLYEKAWVKRGSDIVHGDYIMYDANAEYAEAVGGGKQAGAP
                     DAPKGRVHAVIQSKKKPAGGAPSTTPSPNQ"
     gene            complement(7512..8252)
                     /gene="lptC"
                     /locus_tag="PKW44_01695"
     CDS             complement(7512..8252)
                     /gene="lptC"
                     /locus_tag="PKW44_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HQR50333.1"
                     /translation="MYGRHTIWFPLAILLLLALLTLWIDHAVQPPSPKGDGSSRHDPD
                     YKMENFSAVKTDLNGNPHHLLAAVEMRHFPDDDSTELVRPRFTQYTVDKPYLQIEGQR
                     GLVSSNGENVYFMDNVKAVRGATKDKGELTLVTDYLHIIPDQDLAVTDHPVTITQVPG
                     TVIKANGMELNKRERTLKLSGRVHVHYQKPRAAAGAVATKKNIQKKQASKKSGAQKTP
                     EPSPQRQPNTGNNTARVRRHYENTQTQP"
     gene            complement(8280..8819)
                     /locus_tag="PKW44_01700"
     CDS             complement(8280..8819)
                     /locus_tag="PKW44_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019896963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HQR50334.1"
                     /translation="MNTSENLDAIERAKRVRLVVFDVDGVMTDGSLYLCDDGQEYKAF
                     NSQDGLGMKLLKASGVDMAIITGRTSNVVVKRAENTGVAHFFQGVEDKLEAFEQLARE
                     LGLDHAECAFMGDDVVDLPPMRRCGFAITVPAAPGLVKAHAHYVTSREGGRGAVRDVV
                     EFIMRAQGTFDAQMAQYLK"
     gene            complement(8816..9817)
                     /locus_tag="PKW44_01705"
     CDS             complement(8816..9817)
                     /locus_tag="PKW44_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HQR50335.1"
                     /translation="MVASKLQTESGVAARSLDLAREVLMIEAREVEALAKRLNTNFCR
                     AIDLILQCRGRVVVSGIGKSSHIGAKIAATLSSTGTPAFFMHPAEASHGDLGMITAQD
                     VVIILSNSGESAELLAIVPLLKRMGAKLISLTGSPGSTLAKEADVHLDARVAQEACPL
                     GLAPTASTTAALALGDALAVVLLDARGFSAEDFARSHPGGALGRRLLVHVSDIMRSGD
                     AIPRVSAGTPLTLALLEMTRKGLGMTAVVDADNKPCGIFTDGDLRRAFEAGINVGSTL
                     IDQVMHAGPQSIAAQRLAVEAVELMEQRKINGLLVTDDNGVLVGALNMHDLLLAKVV"
     gene            9934..11907
                     /locus_tag="PKW44_01710"
     CDS             9934..11907
                     /locus_tag="PKW44_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HQR50336.1"
                     /translation="MPSALQSVLILLTSAVCTVALFRVLRLPPMLAYFLIGLALGPHA
                     FGLLPDSESNRELAEFGVVFLMFSIGLEFSLSQLYAMRRTVFGLGGSQVALTMLLVFA
                     AGIVFGIEWRAAFVLAGALAMSSTAIVSKMLVEKVELSSRHGRLAIGVLLFQDLAVVP
                     MLILVPALAEPGNHLAAVIGWSLLKVAATLAVLLVLGKPLVNAWFGLVARQQSRELFV
                     MNVLMVTLALAFTTKLAGLSYALGAFIAGMLISETRYRYQVESDIAPFRDILLGLFFV
                     TVGMLLNVHVLVTQIGWVLLLLVLLVLAKALLVTQLTRMFRHDTGVAVRTGVMLAQAG
                     EFSFVILAQGAEHGLLAGEVLQVVLAATLLSMALAPFIIHHNGRLAAVFSRSYERKRE
                     RWLQGIEHVGGQLSNHVIICGYGRSGQYLSRFLVQENISFIALDIDSERVREAAMAQD
                     NVVYGDAGRRVVLQAAGVARAKALVISYNDTRSSMKILHAMQENYPHVPVIVRTVDDS
                     NMEKFLDAGAAEVVPEVLEGSLMLASHALVLLGVPLSRVVKRIRQFREERYQLFRGFF
                     HGLSDYDLEARDEDQLRLHSVVLPAAAYACGLRLLDVWLESFQVTVKSVRRPNVKGLD
                     PTPDMVLLEGDVVVMQGKPDNLARAEQFLLTGK"
     gene            11904..12950
                     /locus_tag="PKW44_01715"
     CDS             11904..12950
                     /locus_tag="PKW44_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQR50337.1"
                     /translation="MTDRAVVIGAGAVGCLSALALARRGWQVTLVDAGVTGGESSWAG
                     GGILFPLLPWKYGESVNRLALAGAAAYPALSEQLLATTGIDPQYQVSGIRVLADFGGD
                     VALAWCRAHGVTAELSGGDLWLPAVAQARNPRLMQALRASVEVSGIQLMENTGLLPLR
                     ESGGKINRWISTDGRHLEADIFVLTAGAWSTGLLGVKAKAIQVKPMRGQMLLYQLPPG
                     TLQHLLYHDDFYLIPRRDGHILAGSTVEDVGFDKSTTAAAAADLHRKAGRLLPALADA
                     PIIKHWSGLRPGSPDNIPVIARHPDFDNLYLNTGHFRYGITMAPASAEILAAQVGGET
                     LPAWADDYAFPREC"
     gene            complement(12961..13404)
                     /locus_tag="PKW44_01720"
     CDS             complement(12961..13404)
                     /locus_tag="PKW44_01720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR50338.1"
                     /translation="MSESPEDTRHFEQVLSRLNALMKHSRGSLDSPDGMAPATPSAEL
                     FAPQAESGSGTEQAAVLQDALPGTIPLLTDIYEGEIDEASPIALPLQDNSAIVNAFVE
                     ALMPQLLEVLERMLGEEMEKTRATLLSRIEVETSAVLRQHLSNLD"
     gene            complement(13404..13832)
                     /locus_tag="PKW44_01725"
     CDS             complement(13404..13832)
                     /locus_tag="PKW44_01725"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HQR50339.1"
                     /translation="MTRIDFYFNVDHKLRQVAELAMLALPRQRHLFVFTPDAAVADQL
                     EAVLWTRTPTSFLPHCRAGHRLAAETPIVIDWSEAAPPYDDVLVNLRPEQPPFFSRFR
                     SLIEVVGQEQEDREAARARFRFYRDRGYEIRSHDVAGGRA"
     gene            complement(13829..15322)
                     /locus_tag="PKW44_01730"
     CDS             complement(13829..15322)
                     /locus_tag="PKW44_01730"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HQR50340.1"
                     /translation="MEFSIKNGSPEKQRNDCVIVGVFESRKLSAAAEAIDRASGGYLS
                     GILRRGDLEGKLASTLLLHNVPGTLSDRVLLVGLGKEREFREREYHRAVEASVKALDG
                     HGANDAASFLAELPVKKRDIGWKVAQLAETVMDTIYRFSHPSCKKPEERRGIYKLIIN
                     VPQRNDLKKGDAALKKALALASGVRLAKELGNLPGNICTPTYLAQQAGELAQHYGLKL
                     EVLERADMEKLGMGSLLSVTRGSRQPPKLIVLQHLKGKSGQKPIVLIGKGITFDAGGI
                     SLKPAGDMDEMKFDMCGAASVLGTLKAVAEMDLPLNLVVIVPTCENLPDGDASKPGDI
                     VTSMSGQTIEVLNTDAEGRLILCDAMTYAERFDPEAVVDIATLTGACVIALGHHASGL
                     FGNDSALIKELADAGEETLDRAWPMPLWDDYQEQLKSNFADIANIGGRPAGSITAACF
                     LSRFAKKFDWAHLDIAGTAYRSGKQKGSTGRPVPLLAQFLVDRCKRG"
     gene            15387..>15934
                     /locus_tag="PKW44_01735"
     CDS             15387..>15934
                     /locus_tag="PKW44_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HQR50341.1"
                     /translation="MIFKRSLLKELVYTAFATLLVLVGVVIAQRIVYYLGYAARGSLP
                     SDAINALLGFTLLRFLPTLLSLTIFLSVLLTLTRWYRDSEMVVWLSSGLSISAWVRPV
                     LVFSLPVVALIAFLSFVVTPWASAKGMQFLKQMESRDELAAISPGVFKESSHADWVFF
                     VESFNELGNNVKSIFVQSFKHE"
BASE COUNT         2750 a         4691 c         4982 g         3511 t
ORIGIN      
        1 ctttatctca ggaaattcta cttctgatta cgtcagtttt acggggggat taccgggtgg
       61 cgtaagtctg aaataggtca ggggagcagc tttcctccca ttttcctcac tgattgagct
      121 tgagcagcag cggctcgatg cggtcgattt cggcgctgga aaggattttt cgcaccgtgg
      181 ccgccagctt ggggatgtcg ctttgcagca cttggtgctt gaccgccagc acgtgcgccg
      241 ggtgcatgga gaactggcgc aggccgaagc cgagcagcag gcgggtgagt ctggggtcgc
      301 ccgccgtttc gccgcacacc gacaccggga tgccgacctt gtcggcggcg cggatggtca
      361 tttcgatcag ttgcagtacg gctgggtgca aggggttgta gaggtgcgcc acgctgtcgt
      421 cggtacgatc tattgccagg gtgtactgga tcaggtcgtt ggtgccgatg gagaggaaat
      481 ccagctcgcg cgcaaaggct tctgccttga ttgcggcggc ggggatttcg atcatgccgc
      541 caacctggac tgcctcgtcg aacgggatgc tttcctcgcg caggctgcgc ttggcgcgtt
      601 ccagcaggtg cagtgactgg cgcagctccg acaggctgga cagcatgggg atgaggatct
      661 tgacgttgcc gtaatgtgag gcacgcagca cggcgcgcag ttgggtgtgg aagatcatcg
      721 gctcggccag gcacaggcga atagcccgca gtcccagcgc agggttggcg caggtgcgaa
      781 cctcgtcggg attggcaggt ttgtcggcgc ccaggtccag ggtgcggatg gtgaccggca
      841 gacctttcat cgcttcggct accttgcggt aggcctcgaa ctgctcctct tcgtccggcc
      901 attcgcgccg gttcatgaac aggaattcgg tgcggtagag gccgatgccg gtggcgccgg
      961 cggccctggt ctgtgccacg tcttccggga tttcgatgtt ggcgtgcagt tcgatcggcg
     1021 tgccgtccag cgtcaccgcg cgggtcagct tgagccgctt gagcttctgc tgctcgattt
     1081 cccagtgttc ctgcttgagc cggtactcgg tgagggtgtt cttgtccggg ttgacgatga
     1141 ccacgccctg gttgccgtcg acgatgatga gctcgccgtc gtggatcagg tcgcgcgcat
     1201 ggtgcagagc gaggatggaa gggatgttga ggctgcgcgc caggatcgcg gtgtgcgagg
     1261 tggcgccgcc gacgtcggtg atgaaggcgg caaactggtg ctgcttgaac tggatggcgt
     1321 cggctggcga aaggtcgtgc gccaccagga tcatgccgct ttcctgacgc tccagcggtg
     1381 cctggctggg gtggccaagc agcaccttga tgacgcgctc caccacctgt accacgtcga
     1441 acttgcgctc gcgcaggtag tcatcctcga tttcctcaaa ctgccccacc aggtcgtcca
     1501 tctgctgttt aagcgcccat tcggcgttgc acatttcgtc acggatgatc tgcttgggcg
     1561 cttccgaaag ggccttgtcg gctaggatca tgatgtgggt gccgacgaag gaacccaact
     1621 ccgccggcgc attggccggc agggaggcgc gtatgccctc gaggtcgtag cgcacggtat
     1681 ctaccgcttc gccaaagcgg acgacctctg cttcgatcag atgcctgggc acctgataat
     1741 gcgccacctc cagcagggcg tgcgaaacca ggtgcgcctg cccgatggcg atgccgctgg
     1801 ataccgcaac gccgtgcatg gtgaagctca ttgagcgtaa tcctttgaat gtccgaagtt
     1861 catgctattc gccttcgccg aacttgtcgt tgatcagcgc ggcgagtgcc tgcagcgcgg
     1921 cggcctcgtc cggcccgctt gcttccagag tgactttgct gcccttgctg gcggccagca
     1981 tcattacgcc catgatgctc tttgcgttga cacggcggcc gttgcgcgcg atccagattt
     2041 ccgagccgaa tcggctggcg acttgcgtga gcttggtgga ggcgcgtgca tggagcccta
     2101 gcttgttgat gatttcgatt tccagattaa ccatggggca gacatttttc ctgggtgaaa
     2161 tggatgacgc cgccgacgcc gccgccaatg gctttttcta ccagcacttc cagcggctcg
     2221 ttgcggtagg acagtgctcg caccagcatc ggcaggttga cgccagagat gccttccacc
     2281 cggcccggcg tcagcagctg gctagccagg ttgcagggcg tggcgccgta gatgtcggac
     2341 aataccagca ctccgctgcc ctgatcgagc gacttgacca tttcgcgtgc catgggcagc
     2401 agcgcggccg ggtcgccgtg catgccgacg ccgatctgct tcagttgcgg cggttccccg
     2461 cccatgacat gactggcgca gtgcaccagg ctttcaccca gcgagccgtg ggcgataatc
     2521 agtattccaa tcacagtcgt tccccgatca taaaattgat cattccagtt cccggtgcct
     2581 gaccagcacc cgctgcccct gtgttctgaa gtaagcggcc agttgttctg ccaggtaaac
     2641 cgaacggtgc tgcccgccgg tgcagccgag ggccactgtc aggtagctgc ggttgtcgcg
     2701 aacgaagcag ggcagccagt tgccaacgaa ctggcggatg tcgtcggcca tggcctgcac
     2761 gctttcctgg gcttgcagga aagcgatcac cgcctggtcg cgtccggtct gtggtcgcag
     2821 caccgggtcg taatgcgggt tgggcaggca gcgcacgtcg aacacgcaat cggcgtccag
     2881 cgggatgccg tgcttgaagc cgaacgaggc gaacagcaaa gtcagcccgc tgtggtcgag
     2941 tgccatgaaa tccttgaccc aggcgcgcag ggtgttcggc cccaagtcgc tggtgtcgat
     3001 gcgatggcca aggtcgccta gccgggcgag catttcgcgc tcctgatgga tgctttccgc
     3061 cagcgtggta ttgtcgccgg aaagcggatg ccgccggcgc gtttcgctga aacgtttgac
     3121 cagcgtctcc gcactggatt ccaggaacag cacctgcact tccagcccct gtgcccgcaa
     3181 cgaaccgatg atgtccggca gggtttccag cgtactgctg cgggtgtcga tactgaccgc
     3241 gaccctggcc tgctccgcct gctccaggtg ccgcatggtt tccggcaaca gtttggcggg
     3301 caagttgtcg acgcagtaat agccgccgtc ctccagcgcc ttgagcacga cgctcttgcc
     3361 ggagccggac aggccggtga cgacgaccag ttgcatcagt cgccgccctt ttcgatattc
     3421 ctggcgttgc gccggatttc gcggttctgg cgctgggtga actggcgcgt gctgttgatg
     3481 ccgcgcatca gcaggatatg gttgcgcacg gccacttcga ccagtaccgc gatattgcga
     3541 ccggcagcga ccgggatcag gaccttggga accttgacct caaggatgtt ctcgctctgc
     3601 gccttgatct tgaggcgatc gagcatctgc ggcgccaggg tctccgccag ttccagctgt
     3661 acgatcaggt ccagcggctt ggttggtttg acggcgttgt cgccgaacag ctcgcgcaca
     3721 ttgaggatgc ccagaccgcg cacttccagg aaatcctgca gcagcggcgg acatcggcct
     3781 tccaggcgct ccggcgaaat gcggtagaag tccaccacgt cgtcggccac caggcggtgg
     3841 ccgcgtgtaa tgagttccag tgccagttcg ctcttgccga tggccgagtc gcccttgatc
     3901 aggacgccga agccctgcac ctccatgaac acgccatggc agctggccga ttccgccacc
     3961 gcctgtgcca ggtagtggct gagtacgtcc atcaggcgtg gactttgcac cggcgaggtg
     4021 aacagcgggg tcttgcactt ttcggccgca tccagcaatg ccttgggcgg gacttcccca
     4081 ttggcgacga ccactgcggc gagatcggtg gaaaacaggt tggcgatggc ccgcttcata
     4141 tctggaacgt ccaggctgcg caaataatcc atttccgcgc agcccagcac ctgtacccgg
     4201 ttggggtgga cgaagttgag atgcccgatc agcgccagtg aaggcttggt gacggtgtcg
     4261 ctggtgaggt ggttgtcacc cccttccagc cccgccaccc acttcagttt cagattgcgg
     4321 cgggtgtcgc gatacagttc ggcaacgtcg atcttggcca tttttagaat ccaggaatgc
     4381 ggggagggcg cggatttgcg gccttccccc gcatcatcct gccttactcg gcagtctggt
     4441 gcttgatgcc accattggaa cgatggtgat cgccctgctt ttccttgtgc ttgagtacct
     4501 gccggttaag cttgtctgac agcaggtcga tggcgctgta catgttgtcg tcggagcatt
     4561 ccgcgtgcat gtcgttgccg ggcacgtgca gcgtggcctc cactttctgt ttcagcttct
     4621 ccaccgatag ggtgaccttg acgtcaatga cctggtcgaa atggttggtg acacgctcca
     4681 gcttggcttg cacgtagtcg cgcagggcgg gagtaatttc caggtgatgg ccggtgagat
     4741 gcagattcat gatttacctc cttagttgta atacgctcgt cataacgact tgcgctggtt
     4801 ggccgcgggt atctgcaaag actcgcggta ttttgcaatg gtgcgccgcg ccacgacgat
     4861 gccctgttta cctaaaatat cggcaatctg gctgtcggac agcggtttct tcggggtttc
     4921 cccggccacc agctgtttga taagcgcgcg gatggcggtg gcggagcagg cgccaccgga
     4981 gtcggtggaa acgtgactgc caaagaaata cttgagctcg taaacccccc tcggggtgag
     5041 catgtacttg cgcgtagtga cgcgggaaat ggtggattca tgcagttcga gctcgtcagc
     5101 gatttcgcgc agcaccagcg ggcgcatggc gatttcgccg tgttcgaaga aattacgctg
     5161 gcggtcgacg atggcctgtg acacgcgcag gatggtggag aagcgctgct ggatgttctt
     5221 gatcatccat ctggcctcct gcatctggct catcaggtac tggccggaac tttcacgatt
     5281 gcgcttgagg atgtccgcgt acaactggtt gatacgcagc gtgggcatca cctcctgatt
     5341 caggctggca acccacacgc ccttgacctt cttgaccacg atttcatgct ggatgtagtg
     5401 gtcggagctg aggctggtga agcctgccgc cgggcgcgga ttgagctgca tgatcaggct
     5461 ttgcgcctcg cgtagtgcgg cttcgtcaca atgcagcact ttgcgcagct tggcgtaatc
     5521 gcgcaccgcc agcgccggca gatggctttt caccaactcg ctcgccaggg caagctgggg
     5581 ggtatcgggc ggcaagacct gcagttgcag cgacaggcat tcagccaggt cgcgtgcgcc
     5641 gatgccgggc ggatccaggt gctggatgtg tttcagcgcc gttgccagct ccagcgggtc
     5701 aacctcgtat tcgggcggca gaatggacgc cagctcttcc agcggctgct ccagatagcc
     5761 atcctcgttg atggcgtcca ccagcagcgc ggccagggtc aggtcacgct gcgacagcgg
     5821 catcagcttg atttgcccca gcaggtgctg gcgcaaggtg ggcgtgtcgg cttcctggaa
     5881 gacgaaatcg ccttcatcgt cgctgccgga gccggaatcg tcccagcggt tgccgccaga
     5941 gtaatcgctg taatcggtgc cgaattcatc tgcagcatat tcttccggtt tggcgctttc
     6001 ctcctgtggt gtcgcaattt cctgcgccgc ggcaggggcc ggtacaaagc cctcgccttc
     6061 gctcgactcc acccgttcca gcaagggatt ggcctgcagg atggattcca gttcctggtt
     6121 gagttcgacg gtggacatct gcagcaggcg tatggattgc tgcagctgcg gggtcaacgc
     6181 gagatgctgg gaaagtcgaa gctggaggcc ttgtttcatg atggactttg cctacaggcg
     6241 gaaatccttg cccaagtaga cttctcgcac actttcgtta tggacgattt catccgggtg
     6301 tccggcagcc agtaccttgc catcgttgac gatataggcg tggtcgcaga tgcccagggt
     6361 ttcgcgcaca ttgtggtcgg tgatcagcac gccgagtccg cgtccagtca gaaagcggat
     6421 gaccttctgg atgtccagca cagcaatggg gtcgatgccg gcaaagggct cgtccagcag
     6481 gatgaattgc ggattggtgg cgaggcagcg ggcgatctct acccgtcgcc gctcgccgcc
     6541 ggacagactg actgccgaat tgcggcgcaa gtgggtaatg tgcagttcct gcagcaactc
     6601 ctccagttcc tgctccatct ggtcaagggg cagtttgcgc agctccagga tggccaggat
     6661 gttctgctcg acgctgagct tgcgaaacac cgagggctcc tgcggcagat aggacaatcc
     6721 cagccgtgcc cggcggtgaa tcggcagctt gcccagatct ctgccatcga gcaggatgcg
     6781 cccgccgtcg agcggcacca gccccaccat catgtagaaa ctggtggttt tgccggctcc
     6841 gttggggccg agcaacccga cgacctcgcc gctcttgacc gagagcgaaa tgtcctgcac
     6901 cacggtgcgc gccttgtagc gcttgcgcag gttttcgaca atgagttcac tcatggggat
     6961 gcgcttattg gttcggagaa ggggttgtgg atggtgcgcc gcccgctggt ttcttcttgg
     7021 actggatcac cgcgtgtaca cggcccttgg gggcgtccgg cgcccctgcc tgcttgccgc
     7081 cgccgacagc ttcggcgtat tccgcgttgg catcgtacat gatgtagtcg ccgtgcacga
     7141 tgtcgctgcc acgtttcacc caggctttct cgtagagctg taccttgtcc atgcggccat
     7201 cgtactcgat gcgctgggcg ctaccttcca tgtactcgtt cttgccttcc agtttttgct
     7261 tgaaggtggt ggggttgcca gtggaggtgc tgtgctggaa acccaggcta tcctcgcgca
     7321 ccaccagttt atcggcatgg atgaccagcg tgccctgggt caggatcacg ttgccggtat
     7381 aggtgctggt tttcttggca tcgttgacct gcaccgtatc tgcttccagg tcgatcggct
     7441 gttcgcggtc ggctttttct gccagcgcac tgccggggaa cagcagagct gtcagcaaaa
     7501 gcaggtggct gctaaggttg agtctgggta ttttcataat gtctccgtac ccgggccgta
     7561 ttgtttccgg tattgggttg gcgttgagga gatggctccg gggttttttg ggcacctgat
     7621 ttctttgagg cctgtttttt ctggatgttc tttttcgtag caaccgcccc ggcggcagcc
     7681 cggggtttct gataatgcac gtgcacgcgt ccggacagtt tcagcgtgcg ctctcttttg
     7741 ttcagctcca tgccattggc cttgattacc gtccccggca cttgtgtgat ggtcaccgga
     7801 tgatcggtca ccgccaagtc ctggtccggg atgatgtgca gataatcggt caccaaggtc
     7861 agttcgccct tgtctttcgt cgcgccgcgt actgccttta cattatccat gaaatacaca
     7921 ttctcgccat tgcttgatac cagcccgcgc tgcccttcta tctgtagata aggcttgtcg
     7981 acggtatatt gcgtaaagcg cggccgaacc agctccgtgc tgtcgtcatc cgggaagtgg
     8041 cgcatctcga cggcggccag caggtgatgc gggttgccgt tcaggtccgt tttgactgcg
     8101 ctgaaatttt ccatcttgta atccgggtca tgacggctgc taccgtcccc cttgggtgac
     8161 ggcggttgca cggcatggtc tatccatagg gtcagtaacg ccagcagcag cagaatggcc
     8221 agcgggaacc agattgtgtg gcggccatac atgtttggtc ccgctgggct cccgtcctgc
     8281 tacttgaggt actgcgccat ttgggcgtcg aacgtgcctt gcgcccgcat gatgaattcc
     8341 accacatcgc gtactgcgcc gcgcccgcct tccctggagg tgacatagtg ggcatgcgct
     8401 ttgaccaggc cgggcgctgc cggcaccgtg atggcgaaac cgcagcgccg catgggcggc
     8461 aggtctacca cgtcatcacc catgaaggcg cattcggcgt gatccagccc gagttcccgt
     8521 gccagttgtt cgaacgcttc cagcttgtcc tccacgccct ggaagaaatg ggccacgcca
     8581 gtattctccg cgcgcttgac caccacgttg gaagtgcgtc cggtaatgat cgccatgtct
     8641 actccgctgg ccttgagcag cttcatgccc aatccgtcct gcgaattgaa cgccttgtat
     8701 tcctggccgt cgtcacacag atagaggctg ccgtcggtca tgacgccgtc gacgtcgaaa
     8761 acgaccagcc tgacgcgctt ggcgcgttcg attgcgtcca gattttcaga ggtgttcata
     8821 ccactttcgc cagcaataaa tcatgcatgt taagcgcgcc taccagcacg ccgttgtcgt
     8881 ccgttaccag caagccattg atcttgcgct gctccatcag ttccaccgcc tccactgcca
     8941 gacgttgggc ggcgatggac tgcggccctg catgcattac ctggtcgatg agcgtcgagc
     9001 cgacgttgat gccggcttcg aatgcccgac gcaggtcgcc gtcggtaaag atgccgcacg
     9061 gtttgttgtc ggcatccacg acggcggtca tgcccaagcc cttgcgcgtc atttccagca
     9121 gcgccagcgt cagcggagta cccgcgctga cccgggggat ggcatcgccg ctgcgcatga
     9181 tatcgctgac atgcaccagc aggcgacggc ccaaggcgcc gccggggtgg gagcgggcaa
     9241 aatcttctgc ggaaaagcca cgcgcatcca gcagcactac cgccagtgca tcgccgagtg
     9301 ccagtgccgc tgtggtgctg gcggttggcg ccagccccag tgggcaagcc tcctgcgcta
     9361 cccgcgcatc gagatgcacg tcggcttcct tcgccagcgt tgaacccgga ctgccggtca
     9421 agctgatgag cttggcgccc atgcgtttga gcaatggcac gatggccaga agctcggcac
     9481 tttcgccgga gttggagaga atgatgacca cgtcctgcgc ggtaatcatg cccaagtcgc
     9541 cgtggctggc ctccgccggg tgcatgaaga aggctggggt gccggtgctg gacagggtgg
     9601 cggcgatctt ggcgccgata tggctggact tgccgatgcc gcttaccaca acccttccgc
     9661 ggcattgtag aatgaggtcg atggcgcggc agaaattggt attgaggcgt tttgccaggg
     9721 cttccacctc gcgcgcctcg atcatcagca cctcacgcgc cagatcgagc gaacgggcag
     9781 caacgcccga ttccgtttgt aatttcgatg caaccataca acgccagtat aaaagggaat
     9841 gccctgcgaa taaaggacag gtacgcaaat aaccggcttt ccggcacaga ttttgccctt
     9901 attacaattt tgagattatg ttgacgagcc tgcatgccta gcgcactaca atctgttttg
     9961 atcctgctga ccagcgcggt atgcacggtg gcgctatttc gcgtgctcag gttaccgccg
    10021 atgctggcgt attttctgat cggccttgcg ctggggccgc atgcattcgg cctgctgccg
    10081 gacagcgagt ccaaccgcga actggctgaa ttcggtgtcg tgttcctgat gttcagtatc
    10141 ggcttggaat tcagcctgtc gcaactttac gccatgcggc gtacggtgtt cgggctgggc
    10201 gggtcgcagg tggcgctgac catgctactg gtgtttgcgg ccggcatcgt tttcggtatc
    10261 gaatggcgtg ccgcttttgt gctcgctggc gcgctggcga tgtcctcgac ggccatcgtt
    10321 tccaagatgc tggtggaaaa ggtggaactc agttcacgcc acggccgcct tgctatcggc
    10381 gtgctgttgt tccaggatct ggcggtggtg ccgatgctga tcctggtgcc ggccctggcc
    10441 gaacccggca accatctggc ggcggtgatc ggctggtctt tgctcaaggt ggccgccaca
    10501 cttgctgttc tgctggtatt aggcaagccg ctggtcaacg cctggttcgg attggtggcg
    10561 cgtcagcagt cgcgcgaact gttcgtgatg aacgtgctga tggtgacgct ggcgctggcc
    10621 tttaccacca agttggccgg tctgtcctat gccttggggg cattcatcgc cggcatgctg
    10681 atttcggaaa cccgctaccg ctaccaagtg gagtcggaca tcgcgccttt ccgcgacatc
    10741 ctgctcggcc tgttttttgt cacggtgggc atgctgctca atgtgcatgt gctggtgacg
    10801 cagatcggct gggtattgct gctgctggtg ctgctggtgc tggccaaggc cttgctggtc
    10861 acgcaattga cgcgcatgtt caggcacgac accggcgtgg ctgtgcgtac cggcgtcatg
    10921 ctggctcaag ccggcgaatt cagcttcgtg atcctggcgc agggtgcgga gcatgggttg
    10981 cttgccggag aggtcttgca ggtcgtgctg gccgcaacac tgttgtccat ggcactggcg
    11041 ccgttcatca tccatcataa cggtcgcctg gccgccgtgt tttcacgtag ctacgagcgc
    11101 aaacgcgagc gctggctgca ggggatcgag cacgtcggcg gccagttgag caatcacgtc
    11161 attatctgtg gctatggccg cagcgggcag tatctgtcac gcttcctggt gcaggaaaac
    11221 atctcgttca tcgcgctcga catcgattcg gagcgcgtac gcgaagccgc catggcacag
    11281 gataatgtgg tgtatggcga cgcggggcgc agggtggtgt tacaggcggc cggtgtcgcg
    11341 cgcgccaagg cgctggtgat cagctataac gacacccgtt cctccatgaa gattttgcac
    11401 gcaatgcagg aaaattaccc gcatgtgccg gtcatcgtac gtaccgtgga cgacagcaac
    11461 atggaaaaat tcctcgatgc cggtgcggca gaagtggtgc cggaagtcct ggagggcagt
    11521 ctgatgctgg cctcgcatgc gctggtgctg ctcggggtgc cgctgtcgcg tgtggtgaag
    11581 cgtatccgtc agttccgcga ggagcgctac cagctgttcc gtggcttttt ccacgggctt
    11641 tccgactacg acctggaagc gcgtgacgag gaccagttgc gcctacactc cgtggttttg
    11701 ccggcagcag cctatgcttg cggcctgcgc ctgctggacg tgtggctgga gagcttccag
    11761 gtgacggtga agtcggtgcg ccgccccaac gtcaaggggc tggatccgac gcctgacatg
    11821 gtgttgctgg aaggggatgt ggtggtgatg caaggcaagc cggacaatct ggcacgggca
    11881 gaacaatttc tgctgaccgg caaatgacgg accgtgcagt cgtcattggc gccggcgcgg
    11941 tcggctgcct aagcgcgctg gcgctggcca ggcgcggctg gcaggtcacc ctggtagacg
    12001 cgggcgtaac tggcggcgaa tcctcctggg ctggcggcgg catcctgttt ccactactgc
    12061 catggaaata tggcgagtcc gtcaatcgcc tggcgctggc gggcgctgct gcttatcccg
    12121 cgctgagcga acagctgctc gccacgactg gcatcgaccc gcaataccag gtgagtggca
    12181 tacgtgtact ggcggatttc ggcggcgatg tcgcactcgc ctggtgccgt gcgcatggcg
    12241 ttaccgccga gttaagcggt ggtgatttgt ggctgcctgc ggtagcgcaa gcccgcaacc
    12301 cgcgcctgat gcaagccttg cgtgccagcg tggaagtttc tggaatccag cttatggaaa
    12361 ataccgggct gttgccgcta cgggagtcgg gcggaaaaat caaccgctgg atcagtaccg
    12421 acggacggca tctggaggca gacatttttg tactgaccgc cggcgcctgg agcactggcc
    12481 ttctgggcgt gaaggccaag gctatccagg tcaagccgat gcgtggtcag atgctgctct
    12541 accagctgcc gccgggaacg ttgcaacacc tgctgtacca cgatgatttt tacctgatcc
    12601 cgcgacgtga cggccatatc ctggccggaa gcacggtgga ggatgtcggc ttcgacaagt
    12661 ccactacggc ggcggcggcg gccgacctgc accgcaaggc gggtcgtctg ttgccggcgc
    12721 tggcggatgc gcctattatc aagcactgga gcggattgcg gccgggttcg ccggacaaca
    12781 tcccggtcat cgcacggcac ccggatttcg acaacctcta cctcaacacc ggccatttcc
    12841 gctatggcat caccatggcg ccggctagtg cggaaatcct cgcggcacaa gtgggtggcg
    12901 aaaccctgcc ggcgtgggcg gacgactatg cgtttccgcg ggaatgctga gccaggcgga
    12961 ttagtcgagg ttggataagt gctggcgcag gacagcagat gtttccacct cgatgcggga
    13021 tagcagcgtc gctctggtct tttccatttc ctcgccaagc atgcgttcca gcacttccag
    13081 gagttgcggc atcaatgcct cgacgaacgc attcacgatc gcagagttat cctggagagg
    13141 gagcgcgatt ggcgacgctt catcgatttc tccctcgtag atgtccgtca gcagcgggat
    13201 ggtgccgggc agagcgtcct gaagcaccgc tgcctgttca gtgccagaac cggattctgc
    13261 ctgcggcgcg aacagttccg ccgatggcgt tgcaggcgcc atgccatcag gggaatccag
    13321 acttccccgg ctatgcttca tcagggcatt caggcgactg agtacctgct cgaaatgacg
    13381 tgtatcttcc ggcgattcac tcatcaggca cggcctccgg caacatcgtg cgagcgaatt
    13441 tcgtaaccgc ggtcacggta aaaacggaag cgtgcgcgcg ccgcctcgcg atcctcctgt
    13501 tcctgcccga ctacttcgat cagactgcga aagcggctga aaaacggcgg ctgctccggc
    13561 cgtagattga ccaggacgtc atcgtacggc ggggctgcct cgctccagtc gatgacgatg
    13621 ggagtttctg ccgccagccg gtgaccggcg cgacaatgtg gcaggaaact ggtgggcgtt
    13681 ctggtccaca gcaccgcttc cagttggtca gccactgcgg cgtccggcgt aaaaacgaac
    13741 aggtgccgct gacgtggcaa tgccagcatg gcaagctccg ctacctggcg gagcttgtgg
    13801 tcgacattga agtaaaagtc gatgcgggtc atccgcgttt gcaccgatct accagaaatt
    13861 gtgccaacag cggtaccgga cggccagtgg aacccttctg cttgccggag cgataggcag
    13921 tgccggcgat gtccagatgc gcccagtcga acttcttggc gaagcgcgac aggaaacacg
    13981 cggccgtaat actgccggcc ggccggccac cgatattggc gatgtcggcg aaattgctct
    14041 tgagctgctc ctggtagtcg tcccacagcg gcatcggcca tgcgcgatcc agcgtttcct
    14101 caccggcatc cgccagttcc ttgatgagag cgctatcatt gccgaacaat ccgctggcat
    14161 ggtgacccag agcaatgaca caggcaccgg tcagggtagc aatatcgacg acggcttccg
    14221 gatcgaagcg ctcggcataa gtcatggcgt cgcacaggat gaggcggcct tcggcatcag
    14281 tattgagtac ttcgatggtc tggccggaca tgctggtgac gatgtcgcct ggtttgctgg
    14341 cgtcgccatc gggcaggttc tcgcaggtcg ggacgatgac gaccagattc agcggcaagt
    14401 ccatctcggc cacggccttc agcgtgccta acacgctggc tgcaccgcac atgtcgaact
    14461 tcatctcgtc catgtcgccc gccggtttga gcgagatgcc gccggcatcg aaggtgatgc
    14521 ccttgccgat gagcactatc ggtttctgcc cagacttacc cttgagatgt tgcagcacga
    14581 tcagcttggg tggctggcgg ctaccgcgcg ttacgctcag tagcgaaccc atgcccagct
    14641 tttccatgtc cgcgcgctcc agcacctcca gcttgaggcc atagtgctgc gccagttcgc
    14701 cggcctgctg cgccaggtaa gtcggcgtgc agatattgcc tggcaggttg ccgagttcct
    14761 tggccagacg aacgccggag gccagcgcca gggctttctt tagcgcggcg tcgccctttt
    14821 tcaggtcgtt gcgctgtggc acgttgatga tcagcttata aataccgcgg cgttcctccg
    14881 gctttttgca gctggggtga gaaaaccggt agatggtatc catgacagtt tcggccaatt
    14941 gcgccacttt ccagccgata tcccgcttct tgaccggcag ttcggccagg aagctggccg
    15001 catcattggc gccatgcccg tccagcgcct tgaccgaggc ttccaccgcg cggtgatatt
    15061 cgcgttcacg gaactcgcgc tccttgccca gacccaccag tagcacccgg tcgctcagcg
    15121 tgcccggtac attgtgcagc aacagcgtgc tggccagctt tccttccagg tcgccgcgcc
    15181 gcaaaatgcc tgaaaggtag ccgccggagg cgcggtcgat cgcttctgcc gcagcggaaa
    15241 gcttgcgtga ctcgaacacc ccgacaatga cgcagtcgtt acgctgtttc tccgggctac
    15301 cgttttttat gctaaattcc atcgacagag tcctttctac aaacttgtcc gattatccaa
    15361 cgttttggcc tgaattcata cccggcatga ttttcaaacg atccctgtta aaggagctgg
    15421 tttacaccgc cttcgccacg ttgctggtgc tggtcggcgt cgtcatcgcc cagcgtatcg
    15481 tctattacct cgggtatgcc gcccgtggca gtctgcccag tgatgccatc aacgccctgc
    15541 tgggcttcac cttgctgaga ttcctgccca cgctgctctc gctgaccatt tttctgtccg
    15601 tattgctgac gttgacgcgc tggtatcgcg acagcgaaat ggtggtatgg ctcagctcgg
    15661 ggttgagcat ctcggcctgg gtacgcccgg tactggtgtt ttcgctgccg gttgtcgccc
    15721 tgattgcttt cctgagcttt gttgtgactc cctgggcttc ggcaaagggg atgcaatttc
    15781 tcaagcaaat ggaaagccgc gatgaactgg ctgccatctc tcccggggtg ttcaaggaat
    15841 ccagtcatgc tgactgggta tttttcgtcg aaagttttaa tgagttgggc aacaacgtca
    15901 agagtatctt cgtgcaatcc ttcaagcacg agaa
//
LOCUS       DAOMFA010000017        15883 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_290_length_15883_cov_4.704321, whole genome shotgun sequence.
ACCESSION   DAOMFA010000017 DAOMFA010000000
VERSION     DAOMFA010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 15883)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15883)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15883
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_290_length_15883_cov_4.704321"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..157
                     /locus_tag="PKW44_01740"
     CDS             <1..157
                     /locus_tag="PKW44_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HQR50342.1"
                     /translation="HQVKRMVAAAGNRVEALERIAIGGLALPDDLPVGEWRWLEQAAL
                     DKIAGQN"
     gene            253..2619
                     /gene="ppsA"
                     /locus_tag="PKW44_01745"
     CDS             253..2619
                     /gene="ppsA"
                     /locus_tag="PKW44_01745"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HQR50343.1"
                     /translation="MQPYVIPFQHLGIHDVPSVGGKNASLGEMIQHLTKAGVRVPGGF
                     ATTADAYRDFLAQDGLAQRINAELDRLNIEDLTALADCGKKIRQWIMDAPFQEKLQNE
                     IAVAYHQLVGDSNDGTTFAVRSSATAEDLPDASFAGQQETFLNIHGLPHILHAIKEVF
                     ASLYNDRAISYRVHKGFVHADVALSAGIQRMVRSDIGASGVMFTIDTESGFEDVVFIT
                     AAYGLGETVVQGAVNPDEFYVHKPLLGQGKPAVIRRSLGSKQLKMVFTDAQSAGRSTV
                     TLDTSEAERRHFSLDDAEVLELARYAVIIEQHYGRPMDIEWGRDGVDGKLYILQARPE
                     TVKSQATGISQSYVLKQHGKVLASGRAIGQKIGAGPVRVIESAREMHRVQAGDILVTD
                     MTDPDWEPVMKKAAAIVTNRGGRTCHAAIIARELGIPAVVGCGSATETLTENEAVTVS
                     CAEGDTGYVYKGVLEFEVTTTGATQLPKIPTKIMMNVGNPDLAFSFAHIPNEGVGLAR
                     LEFVINNMIGLHPKAVLECHKVRDSVREEIERRARGYASPREFYIEKLTEGIATIGAA
                     FWPKPVIVRMSDFKSNEYKKLIGGDIYEPDEENPMLGFRGASRYAAPEFRDCFELECL
                     AMRKVREEIGLTNVEVMIPFVRTVEEAKAVVEVLAKYGIKRGESGLRLIMMCEIPSNA
                     LLAEQFLELFDGFSIGSNDLTQLTLGLDRDSALVAQAFDERNPAVKALLSMAIGACNR
                     LGKYVGICGQGPSDHPDLAEWLMQQGIQSISLNPDTVVATWLRLAKAS"
     gene            2693..4672
                     /gene="acs"
                     /locus_tag="PKW44_01750"
     CDS             2693..4672
                     /gene="acs"
                     /locus_tag="PKW44_01750"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HQR50344.1"
                     /translation="MSGIESVMTETRLFEPGVDIMRHAAVSGMDAYNALCAEAEKDYE
                     GFWAKLARELLSWHKPFSQVLDESSAPFYRWFGDGELNVSYNCLDRHLATRGDKTALI
                     FEADDGSVTNVTYRQLYHRVCQFANALKTLKLNVGDRVVIYLPMSIEAIVAMQACARL
                     GLTHSVVFGGFSAKSLQERIEDAGARAVITADGQFRGGRAMSLKPAVDEALAMPGCET
                     VEKVIVLKRTGGEVAWNTCDVWWHDLVQGQADTCEPAWISAEHPLFLLYTSGSTGKPK
                     GVQHASAGYLLHAMNTMRWTFDLKDDDVFWCTADVGWITGHTYVTYGPLGMGATQVVF
                     EGIPTYPDASRFWKMIANHKVSIFYTAPTAIRSLIKASETNPATHPRNYDLSSLRILG
                     SVGEPINPEAWMWYYGNVGGGRCPVMDTFWQTETGGHVITPLPGVTPLVPGSCTLPFP
                     GVQAAVVDEAGHDVPWGTGGFLVIKTPWPSMIRTIWGDPERYQKSYFPADLGGKLYLA
                     GDGAVRDAGTGYFTIMGRIDDVLNVSGHRLGTMEIESALVANPLVAEAAVVGKPHDIK
                     GESIVAYVVLKGPRPQGEEAKKVVAQLRDWVGKEIGPIAKPDEIRFGDNLPKTRSGKI
                     MRRLLRSLAKGEEITADVSTLDNPAILEQLKQSVN"
     gene            4750..4825
                     /locus_tag="PKW44_01755"
     tRNA            4750..4825
                     /locus_tag="PKW44_01755"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4783..4785,aa:Asn,seq:gtt)
     gene            4999..5727
                     /gene="rquA"
                     /locus_tag="PKW44_01760"
     CDS             4999..5727
                     /gene="rquA"
                     /locus_tag="PKW44_01760"
                     /inference="COORDINATES: protein motif:HMM:NF038261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodoquinone biosynthesis methyltransferase
                     RquA"
                     /protein_id="HQR50345.1"
                     /translation="MTNLVDHALPGSPFPEKTAQTIPHYLQATYWWAYIHPRAVHVFE
                     RQWLVSLILWGNYKRLCSAVLHGNGQNFHGRTLQIACAYGDLTPRLAERVEGKGTLEV
                     IDVLPIQLNNLARKLPAGGSIRLHCMDSTALQFPDQHFDQALLFFLLHEQPLASRKKT
                     LAEAVRITRPGGTLTIVDYARPSCFNPLRYIMAPILALLEPFARDLWNEEVASWLPSE
                     ANITIVQNQRFFGGLYQMLTLKID"
     gene            complement(5955..8075)
                     /locus_tag="PKW44_01765"
     CDS             complement(5955..8075)
                     /locus_tag="PKW44_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009851982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin oxidoreductase family protein"
                     /protein_id="HQR50346.1"
                     /translation="MSAQLQKTAHGGCPHDCPDTCSMVYEVKDGKLTGVKGNPDHPMT
                     RGGLCVKLKDYEKRHYHPDRLLYPLKRTGPKGSKQFKRISWDEALDEITTRWKAIIAE
                     HGPRAIMPNSYLGNQGLVHGLNGGDAFFNRLGATVCERTFCGEGSCTAWLLTVGPTAG
                     VDPESFIHSKYIIIWACNSVSTNLHHWHIVKEAQKNGAKVVVIDSYASRTAKEANWHI
                     APKPGTDGALAMAMMHVIIGEGLVDQDYVDNYTVGYAELAKRAKDRTPEWAEKITGIP
                     AADIRKLAREYATTPPAAIRLGVALERTYGGGQAIRAVTCLPALIGAWRHVGGGALQF
                     PVWEHPYRFDVICRPDLIPEGTPVVNNLQLGRVLTGEIKLEVPIKSMMCWNTNPVTQA
                     PETDKIIEGLKREDLFLVSAEHFISDTAAYADIVLPAAMGAEMEDMILSWGHLYLTYN
                     AKCIEPPGEALPNNEIFRRLAKRLGFEEDNFKWSDSECLEHYVDWNSPACEGIDLEYM
                     RKHGFARLKVGTKDDRAPHKNGKFPTPTGKCHFSVEGARNFVAGPFRQMYEGFQPGEP
                     LDALPDYVASRETAEANPALAARYPLSIISPKSHGFLNSCYANVKEKIKGQGEQFVLI
                     NFADASARGIQEGDKVRVFNDRGAFEADAKVTGDVNPGIVVATLGYWRQLNNGTVNSI
                     SSADFVDMGHAPTFSDNLVQVALI"
     gene            8231..8794
                     /locus_tag="PKW44_01770"
     CDS             8231..8794
                     /locus_tag="PKW44_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020183833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HQR50347.1"
                     /translation="MAKDMRERILNTASWLFQSRGINATGVDAIVAEAGIAKMTLYKY
                     FPAKEDLILESVRRHSREFTEWLTKRLERTAANPSEKLQQLFDGVEEWLANPQCNGLS
                     FIKASAEFPQPENAVNRLSVELSHEFRDCITNIAREAGIRSAEALGLQLAMLIEGAVL
                     SEQLTKSSDAIEHARQAARILIESSRR"
     gene            complement(8857..9858)
                     /locus_tag="PKW44_01775"
     CDS             complement(8857..9858)
                     /locus_tag="PKW44_01775"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020162928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HQR50348.1"
                     /translation="MTAVEAPFAKLTPDFILDALESAGLRGDGRLLALNSYENRVYQV
                     GMEEGPPLVAKFYRPQRWSDAAILEEHAFVRTLADQEIPVVAALAPDVRGTLLTHDGL
                     RFAVFPRQGGRAPELDDPDTLEWLGRFLGRIHAVGSQTAFRERPALDIASFGEEPATY
                     LLDQRFIPQDLEAAYLAVVSQALDGVRRCFKRAGEVATIRLHGDCHPGNVLWTDDGPH
                     FVDFDDSRMGPAVQDLWMLLSGEREEMAQQLVHLLAGYECFYDFDPRELHLLEALRTL
                     RLVHYAAWLARRWDDPAFPIAFPWFNTQRYWQDRILELREQIALMDEPPLQVASGNR"
     gene            9944..11218
                     /gene="nhaD"
                     /locus_tag="PKW44_01780"
     CDS             9944..11218
                     /gene="nhaD"
                     /locus_tag="PKW44_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter NhaD"
                     /protein_id="HQR50349.1"
                     /translation="MSELLILLFAVTYAAIAFEHPLKVNKSAPALIGAGLMWTIYASF
                     GGNAGAELDASLATVAQIVFFLMGAMTIVEVVDAHDGFDVITDRIRTRKMVPLLWLIG
                     LVTFFLSAVLDNLTTTIVMLSVSRKLLEQKDDRLFAAGIIVIAANAGGAWSPIGDVTT
                     TMLWIGGQISALVIIKKLFLPSLLNLLVPLAVVSYLYRGETIVHPQHAADRHGTRGTA
                     PFVKNFMFFAGIGLLCAVPVFKTLTHLPPFMGVMFALGILWLLGELIHGNKPEQEKQH
                     LTLASALQRIDMASLVFFIGILLSVAVLEHSHILASLASWLDSTIGNQGAIVFIFGLV
                     SAIVDNVPLVAATMGMYSLAQFPTDSFLWEFLAYAAGTGGSILIIGSAAGVAAMGIEK
                     IEFFWYVRKISLLAFAGYASGAVSYILLSQIW"
     gene            complement(11427..13091)
                     /locus_tag="PKW44_01785"
     CDS             complement(11427..13091)
                     /locus_tag="PKW44_01785"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing phosphodiesterase"
                     /protein_id="HQR50350.1"
                     /translation="MIKPSIISVADQELLEEFRLAFVDSKQGIGTEFQQAMQAHGFDE
                     QHPLTGFFSDMLLSDGEALMKLCFGTADGSDSRSIIAIFDSYLACLARQAPLVIKDAV
                     KIRRTMEALRRLAFYYMSMALGLRETAENSRPDAELFDHNTGMLNQEGFTVSLDFLLK
                     DESNWNVAVVLLEFGYSMSMPDAIASLQQEIAQRLMQVARDGDLVARISPKRWILGLR
                     QIKSVAFANLAALKLQRLFEKPMVVAGYPIFLKLRIGVAMAPEYGKQADQLLRVAIEA
                     ARWAATTEDGVQFYSEAVGLRGTREYGLGERLRKALFENALELHYQPQFSMSRNRVVG
                     LEALVRWTLPEGQVPPLLIIGTIEQENIVPQFTQWLIHTSFRNFADFRRQGIETTLSI
                     NLMNQMFALEELPEILTQAANVWKIPLDQVVLEVNEGSLLSDIDTTMWQIERLRETGI
                     SISMDDFGTGYASMTYLSRLEIDELKIDHSFIRAIPLSYRDKNVVRAIINLAVNFNLN
                     IVAVGVETEDIARIIEELGCDIIQGSWVSQPLPPEKLVAWFKQNPL"
     gene            complement(13088..14536)
                     /locus_tag="PKW44_01790"
     CDS             complement(13088..14536)
                     /locus_tag="PKW44_01790"
                     /inference="COORDINATES: protein motif:HMM:NF023299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HQR50351.1"
                     /translation="MDIKVSVNDLKLGMHVAELDRPWADTTFLFQGFILTSESQIEEL
                     ARQCSYVIVSVERSDPALQLDLAVPGGGGAARRGGDVSKARIKGAQAPGESRGGFLSS
                     LKDVVRNALMGLSSSGDTTQGNDGIRGMQEEPKASEKEKQQHINRMTGLLKSFSTDME
                     VHYYENAATLADELTTSRSIHNDVCELASDLIRNMSSKDISERLDLAEGLIKDVVDSM
                     IRNSDAMELISRLKNLDEYSYSKAVNVSVRLIAFGRQLGFPKEQLQQLGMGGLLHDIG
                     RTRLPQNHLKNPFKYTAQEYEVEKLHVSEGLVLLSEGGHISQEVIELVERHHERYDGS
                     GYPNGLKGERIGLFGSMAGIVDSYCAMAADHPYVRAMTSASALRAIIDLSGKRYHPGL
                     VDQFVQTIGIYPPGSLVELSSGEVGVVVRQNRLRRLRPTIMVILDPDHQPYPYPHTID
                     LLYAPMIGDDEVTIRNELGPGAYGINLANYFL"
     gene            complement(14675..>15883)
                     /locus_tag="PKW44_01795"
     CDS             complement(14675..>15883)
                     /locus_tag="PKW44_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQR50352.1"
                     /translation="EVYDFYNYGMAAALVIASVFFPDSDPAAQALNAFLTFGIAFLAR
                     PLGAVLFGHFGDRVGRKTTLAASLLVMGVSTTLIGLLPGYAMIGWIAPALLCLLRFGQ
                     GIGLGGEWGGAALLAMENAPAGKRGWFGMFPQLGPPVGFLFATGSFLLLAWGLDDVQF
                     RDWGWRIPFLVSAILVLVGLYVRLKLAETPVFAKALQQQEKLRLPLAELLSRHAVPLL
                     LGALAMVVCYALFYISTVFSLSYGTTTLGIPRPQFLGMLCVAVVFMAAATPLAAWAGD
                     RYGRRPVLLAASSAALLSGFLLAPTLGSGSAWQITIFLSLELFLMGATFAPMGALLPE
                     LFPTRVRYTGAGAAYNLGGILGASFAPYIAQKLVAHGGLVWVGGYVSVAAAISLLAVF
                     SMRETNDDPL"
BASE COUNT         3434 a         4739 c         4439 g         3271 t
ORIGIN      
        1 ccaccaggtg aagcgcatgg tggccgccgc cggcaatcgc gtggaagctc tcgaacgcat
       61 cgccatcggc ggcctcgccc tgccagacga cttgccggtc ggcgagtggc gctggctgga
      121 gcaggcagcg ctggacaaaa ttgccggcca gaattgaccg cgtgcagcgc taaaaccatc
      181 aatcaggctt ccaccaaacg ctccatatcc ggcacaatag cccccatcgc ctgaatccca
      241 aggaacctcg atatgcagcc ctacgtcatt cccttccagc acctcggcat ccacgacgtt
      301 cccagtgtgg ggggcaagaa cgcctcgctc ggagagatga tccagcacct gaccaaggct
      361 ggtgtgcgtg taccgggtgg tttcgccacc accgccgatg cctaccgcga ttttctggcg
      421 caggatgggc tggcgcaacg catcaacgca gaactggacc ggctcaacat cgaagacctc
      481 accgcgctgg cagattgcgg caagaagatt cgccagtgga tcatggatgc accgttccag
      541 gaaaaactgc aaaacgaaat cgccgtggcc tatcaccagc tggtcggcga ttccaacgac
      601 ggcaccacct ttgccgtgcg ttcctccgcc actgcagagg atctgccgga cgcctccttc
      661 gccgggcagc aggagacctt cctcaacatc cacggcctgc cccacatcct gcacgcgatc
      721 aaggaggtat tcgcctcact ctataacgac cgcgccattt cctaccgcgt gcacaagggc
      781 ttcgtgcatg ccgacgtagc gctgtcggcc ggcatccaga gaatggtgcg cagcgacatc
      841 ggcgcatccg gcgtcatgtt cactatcgat accgaatccg gctttgagga cgtagtgttc
      901 atcaccgccg cctatggctt gggcgaaacc gtggtacagg gcgcagtcaa cccggacgag
      961 ttctacgtgc acaaacctct tttgggccag ggaaagcctg ctgtaatcag gcgcagcctg
     1021 ggttccaagc aactcaagat ggtgttcacc gatgcccaat ccgccggacg ctccaccgtg
     1081 acgctggaca ccagcgaagc cgagcgccgc cacttttccc tagacgatgc cgaagtgttg
     1141 gaactggcac gctacgccgt catcatcgaa cagcactacg gtcgccccat ggacatcgaa
     1201 tgggggcgcg acggcgtgga cggcaagttg tacatcctgc aagcgcggcc ggaaacggtg
     1261 aaatcgcagg ctacaggcat ctcgcaaagc tacgtcctga agcagcacgg caaggtgctg
     1321 gccagcggcc gcgccatcgg ccagaagatc ggcgccggcc cggtgcgggt gatcgaaagc
     1381 gcgcgtgaaa tgcaccgtgt gcaggccggc gacatcctgg tcaccgacat gaccgacccg
     1441 gactgggagc cggtgatgaa gaaggccgcc gccatcgtca ccaaccgcgg cggccgcacc
     1501 tgccacgccg ccatcattgc gcgcgaactg ggcatcccgg ccgtcgtcgg ttgcggtagc
     1561 gccaccgaaa cactaaccga gaacgaggct gtcaccgtat cctgcgccga gggcgacacc
     1621 ggctacgttt acaagggcgt actggaattc gaggtcacca ccaccggcgc gacgcagctg
     1681 cccaagattc cgaccaagat catgatgaac gtgggcaacc cggacctcgc gttcagcttc
     1741 gcccatatcc cgaatgaagg cgtggggctg gcgcggctgg aattcgtcat caacaacatg
     1801 atcggcctgc accccaaggc agtgctggaa tgccacaagg tacgcgacag cgtgcgcgag
     1861 gaaatcgaac gccgcgccag aggctatgct agcccgcgcg agttttacat cgagaagctg
     1921 actgagggca tcgccaccat cggcgcggcg ttctggccca aaccggtgat cgtgcgcatg
     1981 tcggacttca agtccaatga gtacaagaaa ctgatcggtg gcgacatcta cgagccagac
     2041 gaggaaaatc ccatgctcgg cttccgcggt gcctcgcgct acgccgcgcc ggagttccgt
     2101 gactgcttcg agctggaatg cctggccatg cgcaaggtgc gtgaggaaat cggtctcacc
     2161 aacgttgagg tcatgatccc tttcgtgcgc accgtggaag aagccaaagc ggtggtggag
     2221 gtgctggcga agtacggcat caagcgcggc gaaagcgggc tgcgcctcat catgatgtgc
     2281 gaaattccct ccaacgccct gctggcagaa cagttcctgg aactgtttga cggcttctcc
     2341 atcggttcca acgatctgac ccagctcacg ctgggcctgg atcgcgattc cgccctggta
     2401 gcgcaggcct tcgacgagcg caacccggcg gtgaaggcgt tattatccat ggctattggc
     2461 gcctgcaacc gcctcggcaa gtatgtcggc atctgcggtc aggggccatc cgatcacccc
     2521 gacctggcgg aatggctgat gcagcaaggc atccagtcca tctcgctcaa tccggacacg
     2581 gtggtcgcca cttggctgag gttggccaaa gccagctaat ttagtagttt gtacttgagc
     2641 cgtagcttgt tgccatggcg ataaaatcag aatcttggtg gaggagcaga gtatgagtgg
     2701 tattgagtca gtaatgacgg aaacccggtt gtttgaaccg ggcgtcgata taatgaggca
     2761 cgccgcagtt tccggcatgg atgcctacaa tgcgctatgt gccgaggcgg agaaagacta
     2821 cgaaggcttc tgggcgaaac tagcgcgaga actgctcagc tggcacaaac cgttcagcca
     2881 ggtgctggac gagtcgagcg cgccgttcta tcgctggttc ggtgacggcg agctgaacgt
     2941 ttcatacaat tgtctcgacc gccatctcgc cacgcgtggc gacaaaaccg cactgatctt
     3001 cgaagccgac gatggcagcg ttaccaacgt cacttatcgg cagctttatc accgcgtctg
     3061 ccagttcgcc aatgcgctga aaacgctcaa gctcaacgtc ggcgaccgcg tagtgatcta
     3121 cctgccgatg agtatcgaag ccatcgtcgc catgcaggcc tgcgcccgcc tcgggctcac
     3181 ccattccgtg gtattcggcg gcttctccgc taagagcttg caggagcgta tcgaagacgc
     3241 cggtgcgcgc gctgtcatca ccgccgacgg ccagtttcgc ggcggccgcg cgatgtcgct
     3301 caaacccgca gtggatgaag cacttgccat gcccggctgc gaaactgtcg aaaaagtgat
     3361 cgtgctcaaa cgaaccggcg gcgaagtggc gtggaacacc tgcgacgtgt ggtggcacga
     3421 tctggttcaa ggccaggccg acacctgcga gccggcgtgg atcagcgccg agcatccgct
     3481 gttcctgctc tatacctcgg gctctacagg caagcccaag ggcgtgcagc acgcctccgc
     3541 cggctacttg ctgcatgcca tgaatactat gcgctggacc ttcgacctca aggacgacga
     3601 cgtattctgg tgcaccgccg acgtcggctg gatcaccggt catacttatg tgacctacgg
     3661 cccgctgggc atgggcgcta cccaggtagt attcgagggc atacccactt acccggatgc
     3721 cagtcgcttc tggaagatga ttgccaatca taaagtcagc atcttctata ctgcgccgac
     3781 ggcgatccgc tcactcatca aggcctcgga aaccaacccg gccacgcacc cgcggaacta
     3841 cgacctgtcc agcctgcgca tcctgggctc ggtcggcgaa ccgatcaatc cggaagcctg
     3901 gatgtggtac tacggcaacg tcggcggcgg ccgctgcccg gtgatggaca cgttctggca
     3961 gaccgagacc ggcggccacg tcatcacccc gctgcccggc gtcacgccgc tggtgccggg
     4021 ctcctgcacc ctgcccttcc ccggcgtgca ggccgcagtg gtggatgaag ccggccatga
     4081 cgtgccgtgg ggcaccggcg gcttcctagt catcaagacg ccgtggccct ccatgatccg
     4141 caccatctgg ggcgacccgg aacgctacca gaaaagctac ttcccggccg acctgggcgg
     4201 caagctctac cttgccggcg acggcgcggt acgcgatgcc ggcactggct acttcaccat
     4261 catgggccgc atcgacgacg tgctcaacgt gtcggggcat cgcctcggca cgatggaaat
     4321 cgaatccgcg ctggtcgcca atcccttggt cgcagaggct gccgtggtcg gcaagccgca
     4381 cgacatcaag ggcgaatcca ttgtggctta tgtggtgctg aaaggcccgc gcccgcaagg
     4441 cgaggaagcc aaaaaggtgg tcgcccaact gcgcgactgg gtcggcaagg aaatcggtcc
     4501 catcgcaaag cccgacgaaa tccgcttcgg agacaacctg cccaagaccc gctccggcaa
     4561 gatcatgcgg cggctactga ggtcgctggc caagggcgag gaaatcaccg cggatgtatc
     4621 gacgctggac aacccggcca ttctggaaca gttgaaacag tcagtgaatt gactcggcaa
     4681 cgggcttgaa ggcggtgtct cggtgttgac caaaaatccc gaagccatta tgattgcgcc
     4741 tttctcaatt ccccgatagc tcagtcggta gagcaacgga ctgttaatcc gtgtgtccct
     4801 ggttcgagcc caggtcgggg agccagatat gacgaagggt tggcgctcag gcgccgaccc
     4861 ttttgtttta ggtgtgcctg aaatacgccg agggctggat gccggaggga acagtatgtg
     4921 gagaattcca catgacttgc tcatgcaccc aagcgaaccc cgctcgcttt acccctgcgc
     4981 cacaggcaca cgatcaacat gacaaatctc gtcgaccacg ccttacccgg aagccctttc
     5041 cccgaaaaaa cggctcagac cattccccac tacttacagg ccacttattg gtgggcatat
     5101 atccacccga gggctgtgca tgtattcgag cgtcaatggc tggttagtct gattctatgg
     5161 ggcaattaca agcgtctgtg cagtgcggtg ctacatggca acgggcagaa ttttcatggc
     5221 cgcacactgc aaatagcctg cgcttatggt gacctgaccc cacgtctggc agaacgtgtt
     5281 gagggaaagg ggactttgga agtcattgac gtgttgccca tccagttgaa caacctagcg
     5341 cgcaagctac cggcaggtgg atccatcagg ctgcattgca tggattcaac cgcacttcaa
     5401 tttccggacc agcacttcga tcaggccttg ctgtttttcc ttctgcatga acaaccgctg
     5461 gcttcccgca aaaaaacttt ggccgaagcc gttcgcataa cccgtcccgg tggcacgctg
     5521 acaattgttg actatgcacg gccgagctgt ttcaatccac tgcgctatat catggcgccg
     5581 atactggctt tgctcgaacc ctttgcgcgg gacctgtgga atgaagaagt tgcttcctgg
     5641 cttccgtccg aagcaaacat cacgatcgtt caaaaccagc gtttttttgg cggactttat
     5701 caaatgctga ccctgaagat agattaaaac agtcagttaa atttgacaca agttcaagcc
     5761 gcacagccag gtttttgagt tcaagcctta aactttttca tcttgacatt gatcggtgca
     5821 caatccaatc caccaagcca aagtggattg aaaccttgta ttccagatag ataaagattt
     5881 ttctcatgat ttacctctgg agtaaaaagg gtgagctatc tcgctcaccc cgtgaactaa
     5941 ctattccaat agagtcagat tagcgcaact tgcaccagat tgtctgagaa tgtcggcgca
     6001 tgccccatgt ccacgaagtc tgcagaactg atactgttca ccgttccgtt gttgagttgc
     6061 ctccaatagc ccagcgtggc gacgacgatg ccgggattga cgtcgccagt cacttttgca
     6121 tcggcctcga aagcgcccct gtcgttgaat acgcggactt tgtcgccctc ctgaatgccg
     6181 cgtgcgctcg catctgcaaa gttgatcagg acgaattgtt caccctggcc tttgattttt
     6241 tccttcacgt tcgcatagca ggagttcagg aacccgtggc tctttggtga aatgatgctc
     6301 agcgggtatc tggcggcaag cgccgggttg gcttcagccg tttccctaga agctacatag
     6361 tcaggcaggg catctaacgg ctctcccggc tggaagcctt catacatctg gcggaatggc
     6421 cccgccacaa agtttcttgc cccctcgacg gaaaagtggc acttgcccgt aggcgtcggg
     6481 aatttgccat ttttgtgtgg tgcgcggtcg tccttggtgc caactttcaa tcgggcaaag
     6541 ccatgcttgc gcatgtattc caggtcgatg ccttcgcagg caggcgaatt ccagtccacg
     6601 tagtgttcca ggcattcgct gtccgaccat ttgaagttgt cttcttcaaa gcccaggcgc
     6661 ttggccagcc gtctgaaaat ctcgttgttg ggaagggctt cgccaggcgg ttcgatacac
     6721 tttgcattgt aggtgagata gagatgcccc catgacaaaa tcatgtcttc catttccgcg
     6781 cccatggccg ctggcaggac gatatcggca tacgccgcgg tatcggaaat gaagtgttct
     6841 gcggagacca gaaacagatc ctcgcgcttg agtccctcga tgatcttgtc tgtttcaggc
     6901 gcttgtgtga ccgggttggt attccagcac atcattgatt ttatggggac ttcgagcttg
     6961 atttcgccgg tgagcactct gccgagttgc agattgttca ctaccggcgt gccttccggg
     7021 atcaaatcgg ggcgacagat cacgtcaaac ctataggggt gctcccacac cgggaactgc
     7081 aatgctccgc cgccgacatg ccgccaggcg ccgatgagcg cgggcaggca ggtcacggca
     7141 cgtatcgcct ggccaccacc gtaggttctt tccagcgcga cgcccaaacg gattgctgca
     7201 ggcggcgtgg tggcgtattc cctcgccagt ttgcggatgt cggcggcggg gatgccggtg
     7261 attttctcgg cccattcggg cgttctgtct tttgcacgtt ttgccagttc ggcatagcct
     7321 accgtgtagt tgtctacata atcctgatcc actaggcctt caccgatgat gacgtgcatc
     7381 atggccatgg ctagcgcacc atctgtgcca ggcttggggg caatatgcca gttcgcctcc
     7441 ttggctgttc tggaggcata cgaatcgatc acgacgacct tggcgccatt cttctgcgct
     7501 tctttcacaa tgtgccaatg gtgcagattg gtgctgacgg aattgcacgc ccagatgata
     7561 atgtacttgg aatgaatgaa gctttccggg tccactccgg ccgtggggcc gacagtaagc
     7621 agccacgcgg tgcacgaacc ctctccgcag aaagtgcgct cgcacactgt ggcacccaag
     7681 cggttgaaga aggcgtcgcc gccattcaag ccatggacca gcccctggtt gccgaggtag
     7741 ctgttgggca tgatggcgcg tggcccatgc tccgcgatga ttgctttcca tcgcgtcgtg
     7801 atttcatcca gcgcctcgtc ccaactgatt cttttgaatt gcttactgcc cttggggcca
     7861 gttcttttga gagggtacag aagacggtcc gggtgatagt gacgcttttc ataatccttc
     7921 aatttgacgc acaaaccgcc acgtgtcatc ggatgatccg gattgccttt aacgccggtc
     7981 aactttccat ctttaacctc atataccatc gagcaggtat ctgggcagtc atgcggacag
     8041 ccgccatggg cggttttctg taattgagcg gacattgtac gttccccctt gatcttaacc
     8101 agcttttggc aatctgtaat atattccatg taaatacttg gtaatggtca cgcaaacaat
     8161 aaacccgtga aaacaaatat acagatctgt ataattatgt gcaagcccag tttctgttaa
     8221 attaatcacc atggcaaaag acatgcgaga acgtattctg aataccgctt cctggctatt
     8281 tcagtccagg ggcattaatg cgactggtgt tgatgcgatc gtcgccgaag ctggtatcgc
     8341 caagatgacg ctctacaaat atttccccgc caaagaagat ctgattctgg aaagcgtcag
     8401 acggcacagc cgggaattta ccgagtggct tacaaaacgg ttggaaagaa cggctgccaa
     8461 cccatccgag aaacttcagc agttgtttga tggcgtggaa gagtggctag cgaatccgca
     8521 atgcaatggc ctgtcgttca tcaaggccag cgcggagttc ccacaaccgg aaaatgcggt
     8581 gaaccggctt tccgttgaac tttcacacga gttccgcgat tgcattacta atattgccag
     8641 agaggcagga atacgctcgg cagaagcact agggctgcaa ctagccatgc taatcgaagg
     8701 cgcggtgctc tccgaacagc tcactaaaag ttccgatgct atcgagcatg ctcgacaagc
     8761 tgcacgcata ctcatcgaat catcacgtcg ttagctttca aatccggatg ccacatttct
     8821 agccgactac ggcttttgtc tcggccgcta aactgcttac cgattaccgc tcgccacttg
     8881 caggggcggc tcgtccatca gcgcgatctg ctcgcgcagt tccaaaatac gatcctgcca
     8941 gtagcgctgg gtattgaacc agggaaaagc gatagggaat gctggatcgt cccagcggcg
     9001 ggcaagccag gcggcgtagt ggaccaggcg caaggtacgc agtgcctcga gcaaatgcag
     9061 ctcgcgagga tcgaaatcgt aaaaacattc atagccggct aacaaatgta ccagttgctg
     9121 tgccatttcc tcccgttctc ccgacagcag catccataga tcctgcactg ccggccccat
     9181 gcggctgtcg tcaaaatcga cgaaatgcgg gccgtcatca gtccacagca cattgccagg
     9241 gtggcaatcg ccatgcaggc ggatggtggc cacctccccc gctctcttga agcatcggcg
     9301 tacgccatcc agcgcctgcg acaccacggc cagataggcc gcctcgaggt cctgcggaat
     9361 aaacctctga tcaagcagat aggtggcagg ctcttcgccg aaactggcga tatccagcgc
     9421 gggacgttcg cggaacgccg tctgcgaacc gactgcatgg atgcgtccga ggaatcggcc
     9481 cagccattcc agggtatcgg gatcatccag ttccggggcg cgtcctccct gccgggggaa
     9541 cacggcaaaa cgcaagccat catgcgtcag cagcgtgccg cgcacatctg gcgccagcgc
     9601 cgctacgacc ggaatttcct ggtcggccag cgttcggaca aaagcgtgct cttccaggat
     9661 ggcggcatca ctccagcgtt gcggacggta gaacttagcc accagcggcg gcccctcctc
     9721 catgccgacc tgatagacgc ggttttcgta gctgttgagc gcaagcaggc gaccgtcgcc
     9781 gcgcaggccg gcgctttcca gcgcgtccag gatgaaatcg ggggtcagtt tggcgaatgg
     9841 ggcttcaaca gcagtcatgg cgccattcta atgccgggcg ggtcgttgtc ggtgctaaaa
     9901 tccggccatc tacgaaaaag ccagcccagc gaaggccaac accatgtcgg aactcctgat
     9961 ccttctcttt gcagtcactt acgcagcgat tgccttcgaa cacccgctta aggtcaacaa
    10021 aagcgcgccg gcactgatag gcgccgggct catgtggacc atttacgcga gctttggcgg
    10081 gaacgcaggc gcagaactgg acgcgtccct agcgacagtg gcccagattg tcttcttcct
    10141 gatgggggcg atgaccatcg tcgaggtcgt cgatgcgcac gatggcttcg atgtgattac
    10201 ggatcggatc cgcaccagga agatggtgcc gctgctctgg ctgatcgggc tggtcacctt
    10261 ctttttgagc gcggtactcg acaatctcac gacgaccatc gtcatgcttt ctgttagccg
    10321 aaaactgctg gagcaaaagg atgacagatt gttcgcggcg ggaatcatcg tcatcgcggc
    10381 gaacgccggc ggcgcctggt ctcccattgg cgacgtcacc accaccatgc tctggatcgg
    10441 tggccagatt tccgccttgg tcatcatcaa gaagctgttt cttccctcgc tgctcaatct
    10501 tctggtaccg ctggcggttg tcagttacct gtatcgcggg gaaacgatcg tccacccgca
    10561 gcacgcggca gacaggcatg gtacccgcgg cacggctccc tttgtaaaga acttcatgtt
    10621 ttttgccgga atcggcctgc tgtgcgccgt tccagtgttc aagacattga cccatttgcc
    10681 gccttttatg ggggtgatgt tcgcgctcgg catcctctgg ctgctcggcg agctgattca
    10741 cggcaacaag ccagagcagg aaaagcagca cctgaccctc gcgtccgcct tgcagcgcat
    10801 cgacatggca tcgctggtgt tctttatcgg gattctgctg tccgtggcgg tgctggaaca
    10861 cagccatatc ctggcgtctc tggcatcgtg gctggacagc acgataggca accagggcgc
    10921 gatcgttttc atttttggtc ttgtcagtgc gatcgtggac aatgtgccac tggtcgccgc
    10981 gaccatggga atgtactctc tggcccagtt tccgactgat tccttcctgt gggagtttct
    11041 cgcgtacgcc gcgggaaccg gcggatcaat tctgatcatc gggtccgctg cgggcgttgc
    11101 cgccatgggc atcgagaaga tcgagttttt ctggtatgtc cgaaaaatca gcctgctcgc
    11161 attcgccggc tacgcaagtg gagcggtttc atatatcctg ctgagccaga tctggtagct
    11221 tgcaatcgag ggcagcagcc ccctctctgg cgtgatgatt tcgctcgaac gcaacaagac
    11281 cctttgcacc catttcagct tctggccggc gattctcgtc aatttactgg tttcgctcgg
    11341 ggcatggtac atacagttcg aaggtttatc ggcataatca acagcgtctg acagaatcaa
    11401 acgtcgaacc ttagcgccaa gaaggttcaa agcggattct gtttgaacca cgctaccagt
    11461 ttttccgggg gcagcggctg gctgacccag gaaccctgaa tgatgtcgca gccaagctct
    11521 tcgatgatgc gcgcgatgtc ttcggtttca acgccgactg ccacgatgtt gaggttgaag
    11581 ttgacggcca gattgatgat cgcacgcacg acattcttgt cccggtagga taaggggatg
    11641 gcccggataa agctgtggtc aatcttgagt tcgtcgattt ccaggcggct gagataagtc
    11701 atggaggcat agccggtgcc aaaatcatcc atcgaaatgc tgatgccagt ttctctcagt
    11761 cgctcgatct gccacatggt ggtgtcgata tcgctgagca acgaaccttc gttgacctcg
    11821 aggaccacct gatcaagcgg tatcttccac acatttgccg cctgagtcag aatttccggc
    11881 aactcctcca gagcaaacat ctggttcatc aagttgatgc tgagcgtggt ttcgatgccc
    11941 tgccggcgaa aatccgcgaa gtttctgaat gatgtatgaa tcagccattg agtgaattgg
    12001 gggacgatat tttcctgctc gatggttccg atgatcagaa gcggcggcac ctgcccctcg
    12061 ggcaaggtcc agcgtaccag cgcctccaac ccaaccaccc ggttccgcga catggaaaac
    12121 tgcggctggt aatgcagttc cagcgcgttt tcaaacagcg ccttgcgcag cctttctccc
    12181 aagccatatt cacgggtacc tctcaagccc acggcctcgc tatagaactg aacgccgtct
    12241 tcggtcgtgg cagcccagcg ggctgcttcg attgccacac gcaacaattg atccgcctgt
    12301 ttgccatact ccggggccat tgccacaccg atgcgtaact tgagaaaaat cgggtacccg
    12361 gcaacgacca taggtttttc gaacaagcgt tgcagtttga gggctgcgag attggcaaaa
    12421 gcaacggatt tgatctgccg caatcccaat atccaccgct tgggtgaaat gcgagctacc
    12481 aggtcgccat ccctggctac ctgcatcaac cgctgcgcga tctcttgctg cagggaggcg
    12541 atcgcgtccg gcatgctcat gctgtagcca aattcgagga gaacgaccgc cacattccag
    12601 ttgctttcgt ccttcagcag aaaatcgagg gaaacagtaa aaccctcctg attgagcatg
    12661 ccggtgttat ggtcaaaaag ctctgcatca ggccgcgaat tttcagcagt ttcgcgcagc
    12721 cctaaagcca tcgacatgta ataaaaagcc aaccgccgca acgcttccat ggttcggcga
    12781 atttttacgg catccttgat aacaagaggc gcctggcgcg ccaggcaggc aagatagctg
    12841 tcgaagatgg cgatgatgct gcgactgtca gacccgtcgg cggtgccaaa gcaaagcttc
    12901 atcaacgcct cgccatcact cagcagcata tccgagaaaa atcccgtcag cggatgctgc
    12961 tcgtcaaagc catgggcctg catggcctgc tggaactctg ttccaatacc ctgcttgctg
    13021 tcgacgaacg cgagacggaa ctcctccagc aattcctgat ccgcaacgga gataatggaa
    13081 ggcttgatca caggaaatag ttcgccaggt taatgccgta ggcaccagga ccgagttcgt
    13141 tacgaatagt gacttcgtcg tccccaatca tgggggcata cagcaggtcg atggtatggg
    13201 ggtatggata gggttggtga tccgggtcca gaatcaccat gatcgtcggg cggagtcgcc
    13261 gcaggcggtt ttgtctgacg accacaccca cttcgccgga actcagctcg accaaggagc
    13321 ctggcggata aatcccgatg gtttgcacga actggtcgac caggccagga tggtatctct
    13381 tgccgctcaa gtcaataata gcacgtagcg cactggctga agtcatcgcg cggacatatg
    13441 gatgatcggc cgccatggcg caataggaat ccacgatccc tgccatgctg ccaaacaggc
    13501 caattctctc acccttaagg ccattggggt agccgctgcc gtcgtatcgc tcatggtggc
    13561 gctccaccag ttcgatcact tcttgagaga tgtggccccc ttcggacagc aaaacaaggc
    13621 cttcggaaac atgcagtttc tcgacctcat actcctgtgc cgtgtatttg aaggggtttt
    13681 tgaggtgatt ctggggaagc cgtgtgcgtc cgatgtcatg cagcaacccc cccatcccca
    13741 actgctggag ctgttctttc ggaaagccaa gctgtcggcc aaaagcgatt agccgcaccg
    13801 acacgttgac tgccttgcta taggagtact cgtccagatt cttcagccgg gaaataagct
    13861 ccatggcatc ggaattacgg atcatgctgt ccacaacgtc cttgataagg ccttccgcca
    13921 gatccaggcg ctcgctgatg tccttgctcg acatgttccg gatcaaatcg gaagccagtt
    13981 cgcagacgtc attgtggatg ctgcgtgaag tcgtcagctc gtctgccagg gtcgcggcgt
    14041 tttcgtaata atgaacttcc atgtcagtgg aaaaggactt gagcaaaccc gtcattctgt
    14101 tgatgtgctg ctgtttctct ttttccgacg ctttgggctc ctcctgcatg ccgcgtatgc
    14161 catcgttacc ttgcgtggtg tcaccgctgc tggaaagccc catgagggca ttacgtacga
    14221 cgtccttcag gctggaaagg aatcccccgc gggactcccc tggagcctgt gctcccttga
    14281 tacgagcctt gctcacatcg ccgccacgac gtgccgcacc gccgccaccc ggtaccgcca
    14341 aatccagttg caatgcaggg tcggagcgtt cgaccgagac aatgacataa gagcactgcc
    14401 gggccagctc ttcgatttgg ctttccgatg tcagtatgaa cccctggaac agaaaggtag
    14461 tatccgccca gggacggtcg agctccgcga catgcatgcc gagcttcaga tcgtttacgc
    14521 taaccttgat gtccacaaaa accgcgcccc ttattactgc actatccgga tacaaagctt
    14581 acacgaaaac ccttttgcag tagctgccca tgacctgaaa attgcaggaa gaaacgcaaa
    14641 attgctgctg cttttctccc ccagccccgc cctatcacaa ggggtcatcg ttcgtctccc
    14701 gcatggaaaa aaccgccagc aaactgatgg cggcggccac cgacacgtag ccgcccaccc
    14761 agaccagccc gccgtgggcg accagcttct gcgcgatata gggcgcgaag gaagcgccga
    14821 ggatgccgcc cagattgtag gccgccccgg cgccggtata gcgcacacgg gtagggaaca
    14881 gttccggcag caaggcgccc ataggggcaa aggtggcgcc catcaggaac agttcgaggg
    14941 aaaggaatat ggtgatctgc caggccgagc cgctgcccag cgtgggcgcc agcaggaagc
    15001 cggacagcag ggccgcacta ctggccgcca gcaataccgg gcggcgcccg tagcggtcac
    15061 ccgcccaggc cgccagcggc gtggccgccg ccatgaacac taccgccacg cacagcatgc
    15121 cgaggaactg cgggcgcgga atgcccagtg tggtggtacc gtagctcaac gagaacaccg
    15181 tggagatgta gaacagcgcg tagcacacca ccatcgccag cgcgcccaat agcaagggaa
    15241 cagcatgacg actcaacaat tcggccaaag gcagtcggag tttttcctgc tgttgcagcg
    15301 ccttggcgaa caccggcgtt tctgccagct tcagccgcac atagaggccg accagtacca
    15361 gaatagcgct gacaaggaag ggaatacgcc agccccagtc acggaactgc acgtcatcta
    15421 gcccccaggc cagtagcaga aaactgccgg tggcgaacag aaaccccacc ggcggcccca
    15481 gttgcgggaa catgccgaac cagccgcgct tgccggccgg cgcattctcc atcgccagca
    15541 gcgctgctcc gccccactcc ccgccaagcc caatgccctg gccaaaacgc agcagacaca
    15601 gcaacgctgg tgctatccag ccaatcatcg cgtacccggg cagcaggccg atcagagtgg
    15661 tggaaacgcc catcaccagc agcgaggccg ccagggtggt tttgcgcccg acgcgatcac
    15721 cgaaatgccc gaacaacacc gcgcccaggg gacgggcgag aaaggcaatg ccaaaagtca
    15781 ggaaagcatt tagcgcctgc gccgccgggt cactgtccgg aaagaacaca gacgcgatca
    15841 ccagcgccgc cgccatgccg tagttgtaga aatcatagac ctc
//
LOCUS       DAOMFA010000018        15510 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_308_length_15510_cov_5.185765, whole genome shotgun sequence.
ACCESSION   DAOMFA010000018 DAOMFA010000000
VERSION     DAOMFA010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 15510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15510
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_308_length_15510_cov_5.185765"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..872
                     /locus_tag="PKW44_01800"
     CDS             <1..872
                     /locus_tag="PKW44_01800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR50353.1"
                     /translation="GEAVCRRIGVDPGIECKALDSVELAQRLGLGPQQLRAFPELLYM
                     QVLAKGGASVNLASQEQTRSYWIQRLSGILWLSAATLLLGGMLVAALNVFNTMDYKAQ
                     TETARTQTRLQEDMYNEVARNFPSTPLNGENLRIAVEMSDTIAANAKTPLRLMQAVSA
                     SLDNAPEILLQRLRWKLTTDINSKDDAVQSQAQAAQGVSESGTLYETGFLDGEIRNFQ
                     GDYRAALNSVNRLADLMRKDPRVGQVQVVQQPVNVSSQTSLQGSTLDAAAQQMPAAQF
                     KLKVILKPEVTQP"
     gene            869..1486
                     /locus_tag="PKW44_01805"
     CDS             869..1486
                     /locus_tag="PKW44_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR50354.1"
                     /translation="MKLNAKDWQTLQPPLLALAAVLLVVFLLNWYTIRQQDLAFQAMQ
                     AQQSQLEQARRHYQESGAEKEAIIKYLPHYQDLIDRGFVGEERRIEWIDHLRNVNQQY
                     KLFGITYSIGSQEKYKPSFSLNSGPFELHRSAMKIESALLHEGDLLTIINAMSAANQA
                     PFTLRDCTITRIATAARNKFLPNLNASCEIDWLTITEPQKTGVTP"
     gene            1483..2070
                     /locus_tag="PKW44_01810"
     CDS             1483..2070
                     /locus_tag="PKW44_01810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR50355.1"
                     /translation="MTPLRHVMFLACLIPALASADDQFGRLFMTPAERNNLNYLRQTS
                     RPPEKIVAAEQPPDEGTEKAVATPVAPSAVTMQGYVKRSDGKATVWVNRQPVQETSRH
                     GEIEVGKLGASGDRVRVKVPGTGQTVELKAGQTYDPASGKVVENARELSNAEAMSSGA
                     PPAKKSAAEDSLAGKEDQHPQVTEVPSMPSASKKP"
     gene            2112..3257
                     /locus_tag="PKW44_01815"
     CDS             2112..3257
                     /locus_tag="PKW44_01815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR50356.1"
                     /translation="MVLIMLLFIVGLVATVYLVHALDGNAVKIERDQKTAESLALAKA
                     AIIGWAATRTTLTQLGQLPCPEDTTLIGLPTEGNAKSACTLPAVGRLPWRSLGLGELR
                     DGNGDNLWYAISPGFRSTTINSDTPAQLTVDGVAGSAIAIVFSPGTILAGQTRPIPTI
                     ANPPDVTQYLDLSNNDGDSIFVTSGAAGSFNDRLLTISHNELFAVVEKRVASEVLKCL
                     DVYASAPVAVPPPVPPTGAYPWPAMLDTVMPPTYVGTVNNFFGRVPDSPMGGNWSGAC
                     AIPVGGTGWWLNWKEMVFFALADGYKPTIATPGCGACLTVLPPTATADKRVVVLVAGQ
                     KLAGQMRSTPADKGTLSNYLETPNSTGGVSFAKQNMTSGFNDVVAYH"
     gene            3279..3995
                     /locus_tag="PKW44_01820"
     CDS             3279..3995
                     /locus_tag="PKW44_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020183440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HQR50357.1"
                     /translation="MKDSQGFSLVELAVVLVIVGLLLGGLLMPLSAQMDVRGYQDTSK
                     RLEEAKEAIIGFAMANGRLPCPASSTSLGIEDPGGGGDCNHDYDGFLPAATLGLSNVD
                     GGGYAMDGWSGNSVNRIRYAVTTANASAFTTPGGMRGIGMATLAPALRVCTSGSGVTA
                     TTCGTATSLTTTAVAVIFSAGKNASTGGTGTDESKNYDDDQVFVSHEPVPGANEFDDS
                     VTWISPNLLYSRMVAAGQLP"
     gene            4006..4758
                     /gene="moeB"
                     /locus_tag="PKW44_01825"
     CDS             4006..4758
                     /gene="moeB"
                     /locus_tag="PKW44_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HQR50358.1"
                     /translation="MNDHQLLRYSRHILLPQIAYEGQEKLAQSHALIVGAGGLGSPVV
                     LYLGAGGVGKLTICDYDDVDFSNLQRQVIHTTQSVGVNKALSAQQALAVINPEITVVP
                     IPRKSTEDEFTELARDADVVIDCSDNFQTRYALNRICVQLKKPLVSGAAIGFEGQVSV
                     FDMRHADSPCYHCLFPDVGAEDGVGEMRCAEFGVFAPLVGMIGTTQAAEAMKLLLGVG
                     ESLQGRLLLLDVLAMEWRSIQLKRDPACGVCA"
     gene            complement(4778..5716)
                     /locus_tag="PKW44_01830"
     CDS             complement(4778..5716)
                     /locus_tag="PKW44_01830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2157 domain-containing protein"
                     /protein_id="HQR50359.1"
                     /translation="MSTRQDAIIEVVDIIKRHNLSLVEIAEALKGGLEIKAERSSGIL
                     SRLFGYIGGIFVFAGLAIYIGMQWSSLSPASRILLTLGTGFSVFVLALVCTTDDRFEK
                     AATPLFLVAALLEPTGILVTLHEFSRGGDPAYGLLFLNLVMAIQQGCAFYARRRSVLA
                     VTAVYFSVGFFVITFDLLHVERHLIGLTIGLSLLSIGWSIDKSRHKPVAALVYFVGAI
                     LFLATAYDWLDHRPYQILFLALACGTIFLSTVARSRTLLLIGTLALIGYIGKYMAEHF
                     AHNLAGPVGLMLAGFLLIAVGALAVRINNRYIREKR"
     gene            complement(5713..6318)
                     /locus_tag="PKW44_01835"
     CDS             complement(5713..6318)
                     /locus_tag="PKW44_01835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR50360.1"
                     /translation="MAFKDFVKNNFVLIVGLALPVLLMFGFMIAGSLPNIVTDPPKYD
                     LVFAVADVGSNNLPIALKLTVDKDGTLKAQYTPNRKEGHLYNAWKKLYIYQAKTQKVR
                     ELPFPYPADMEKIVDVKEEIVEATKELKLDTTSESPDGYNLSYDGYSHSGLVGELFMG
                     NRYSSEPQLRKGGSAIRLVSGNARTNFYYGNIEFIGWVMDK"
     gene            complement(6380..7222)
                     /locus_tag="PKW44_01840"
     CDS             complement(6380..7222)
                     /locus_tag="PKW44_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012777619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HQR50361.1"
                     /translation="MFEFTLVLLFPVVVGIWMGVRIVPQGEEWVVERLGRFHVVLNPG
                     LSIIVPFIDQVRYKVTTKDIILDIPEQEVITRDNAVILANAIAFLKVSNIERAVYGIE
                     NFREAMRNMVQTSLRSIIGGMDLNSALSSRDRIKAELKTAIADEAIDWGLTVKSVEIQ
                     DIKPSPNMQKAMEMQASAERERVALVTRSEGEKQAIILNAEARLEAARRDAEAQEVAA
                     KASAEAMRLIGEAVKDSNASATFLLGDRYIQAMQKLSTSENSKLVLLPGDLVGAVKSL
                     LGSK"
     gene            complement(7233..7679)
                     /locus_tag="PKW44_01845"
     CDS             complement(7233..7679)
                     /locus_tag="PKW44_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HQR50362.1"
                     /translation="MLQPLWAWAILGLVLLGAEMLSGTFYLLWFGIAALCVALLLALW
                     PDAPFSLQLLAFSVLALTSLTVWKLKYHGKVADLRIGQSHGDAIGQAGRISATVAPEQ
                     LGRITFTMPVMGSREWTAVSDERIEAGETAVVSGIEGNYLRVNRAN"
     gene            7780..8346
                     /locus_tag="PKW44_01850"
     CDS             7780..8346
                     /locus_tag="PKW44_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019899882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HQR50363.1"
                     /translation="MVSVIVVAVLVVALVYGVMLYNNLVLLKNNVAKAWANIDVLLKQ
                     RHDELPKLIDTCKQYMKYEQETLEKVIQARSRVADARESRNIAALGAAEGALRAGLGS
                     LFALVESYPELKANEQFLHLQGRITGLENAIADRREFYNESANINNVRIEQFPDLIVA
                     RLFNFSDAEMLRFEAAELEDVDVARRFG"
     gene            8381..9286
                     /locus_tag="PKW44_01855"
     CDS             8381..9286
                     /locus_tag="PKW44_01855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR50364.1"
                     /translation="MCEALKSGYSNLITSCGHLVLILVALRINQPPVWVACLGLISVI
                     SFVAWASNLRRNRAIADTPTSRVASAAQGYVELYGPAVRGAEFLARSRLSGMPCVWYR
                     HVTYQKNSDGEWAEIARGTSDTLFALEDGSGRCLIDPDHAEVITTHSRTWIEGDYKHI
                     EEQLFASDNIYALGEFTTVGGANAHLDLKQDVVAMLAEWKKDQLSLLKRFDLNRDGQI
                     DLQEWELARRAAIREVEKQHRDLRMQPGVHVMRAPSGGQMYLLSNLSPQQLKNRYVRW
                     GWIHLLTFFAAAAGTASIGLGAALW"
     gene            9298..12108
                     /locus_tag="PKW44_01860"
     CDS             9298..12108
                     /locus_tag="PKW44_01860"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HQR50365.1"
                     /translation="MSELDKSFEPRNIENRWYSFWEDRGYFAAGLDTEKSDNFCILLP
                     PPNVTGTLHMGHGFNQTLMDALTRYHRMKGDNTLWQPGTDHAGIATQIVVERQLDAQG
                     LSRHDLGREKFLEKVWEWKEHSGSTITRQMRRLGTSPDWSRERFTMDAGLNKVVTETF
                     VRLYNEGLIYRGKRLVNWDPKLHTAVSDLEVVQEEEDGSLWHIRYPLADGSESLVVAT
                     TRPETMLGDTAVMVHPEDERYAHLIGKTVKLPLTEREIPIIADDYVDREFGTGVVKVT
                     PAHDFNDYAVGQRHQLPMISILTLDARVNEHAPEKYRGMDRFDARKVIVADLEALGLL
                     VKIDKHKLKVPRGDRTGVVIEPMLTDQWFVAMSKPGANGKSITEQALEVVANGEIKFH
                     PENWVNTYNQWLNNIQDWCISRQLWWGHQIPAWYGVNGEVFVAHDEAEARHLADEEGY
                     AGPLTRDEDVLDTWYSSALWPFSTLDWTPEWPAKSNPALDLYLPSSVLVTGFDIIFFW
                     VARMVMMTKHITGKIPFRDVYVHGLIRDAEGQKMSKSKGNVLDPIDLIDGINLDDLVK
                     KRTTGLMNPKQAEQIEKRTRKEFPEGIPAFGTDALRFTFASLASPGRDIKFDMHRCEG
                     YRNFCNKLWNATRFVLMNCEGQDCGMEPCSHDCGPEGYLDFSQADRWIVSLLQHTETE
                     VEKGFSDYRFDYVAQAIYKFVWDEYCDWYLEIAKVQLQNGSDAQQRATRRTLLRVLET
                     VLRLAHPVIPFITEELWQTVSEYTGQQADTIMVRPYPKSQPEKIDEAAEAWVAMLKQA
                     VDACRSLRGEMGVSPATRVPLIAAGSDVLTAYAPYLKALAKLAEVEVVNELPEADAPV
                     AVVGDYRLMLKIEVDVAAEKERLGKEVARLEGEIAKANAKLSNESFVARAPAAVVAQE
                     KERLAGFMAMLEKLQAQLVRLK"
     gene            complement(12170..12634)
                     /locus_tag="PKW44_01865"
     CDS             complement(12170..12634)
                     /locus_tag="PKW44_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HQR50366.1"
                     /translation="MSKKLALIASKGTLDWAYPPFILASTAAALDYEVQIFFTFYGLT
                     LLKKELDARISPLGNPAMPMPVPMPNLVQMLPGMEGMATMMMKQKMKDKGVASLEELR
                     SLCLEAGVKLIACQMTVDLFDFKKEDFIEDIEFGGAATFFEFAGEADVSLFI"
     gene            complement(12639..12869)
                     /locus_tag="PKW44_01870"
     CDS             complement(12639..12869)
                     /locus_tag="PKW44_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HQR50367.1"
                     /translation="MEFDKEVDARGLNCPLPILRVKKALADMTGGQVLKTVATDSGSV
                     KDFQAFCRQTGHELLESSEANGEYTYFMKKKA"
     gene            complement(12880..13203)
                     /locus_tag="PKW44_01875"
     CDS             complement(12880..13203)
                     /locus_tag="PKW44_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HQR50368.1"
                     /translation="MSGYQDIDATYLQSLLGLHNVLLVDVRNNEEVARGVIPGAVHIP
                     LSLIPVKADELGGEQPLVFYCHAGIRSAQACAFMASKGRDNIFNLQGGVIAWGKAGHP
                     FEPKQ"
     gene            13363..13941
                     /locus_tag="PKW44_01880"
     CDS             13363..13941
                     /locus_tag="PKW44_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HQR50369.1"
                     /translation="MGDDSSYLSTLQDYYAEQRVMPSFAKICQLLKFSSRASAAALVA
                     RLKLQGFLEATETGRLKPGKRFFERILADDVVRAGMPSPAYSGSSDTLTIDEYLVDQP
                     SKTLLIPVKGDSMVDAGILPDDIVVVEKRQLASVGDIVVAIVDGEFTLKTLGREKGEF
                     VLYPANKAYPVIRPEGQLEIFGVVVGQFRKYG"
     gene            complement(13981..>15510)
                     /locus_tag="PKW44_01885"
     CDS             complement(13981..>15510)
                     /locus_tag="PKW44_01885"
                     /inference="COORDINATES: protein motif:HMM:NF017197.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HQR50370.1"
                     /translation="EFHLVDKSNSSDLLKWKTLDLDNVQVGANTGETPYDIAIAGLAL
                     NDFYARIVVNEDGRLALRKLVKGRSRTGELLTDEESAPPAETNASQTQQDAGKSAVPV
                     ATAPAQPSPFKLRLDKVTLSGGDINFSDFFIKPNYSADLSEIIGSVSGLSSEPGSTAD
                     VDLRGKVNGTAPLEIAGKINPLAKDIFVDLKASAKGIELSGINPYATRYAGYAIEKGK
                     LSVDIHYFIKDRKLQAENHVFLDQLTFGQKVESPDATKLPVTLAIALLKNSKGEIDIN
                     LPIQGSLDDPQFSIGSIVWKMIGNLIVKIVTSPFALLGSLFGGGDQQLEYLEFDPGLA
                     TISKTGEEKLQTLAKALNDRPALKLDIAGRADPETDREGLKQAGLQHKVKAQKLKNTV
                     KKGEDSASVDSVTISAEEWPVYLKQAYKEEKFPKPRNLIGFAKDLPPAEMEKLILTNI
                     TVGEEDLRRLANQRADAVSTWLTKAGSVAAERVFITPPKLNSEGIKDKGKPNRVDFSL
                     K"
BASE COUNT         3177 a         4387 c         4736 g         3210 t
ORIGIN      
        1 aaggcgaggc agtctgccgt cgtatagggg tggatcctgg catcgagtgc aaggcgctgg
       61 actcagtgga gctcgcgcag cggctcgggt tggggccgca acagctgagg gcatttccgg
      121 aattgctcta catgcaggtg ttggcgaaag ggggggcgtc agtcaatctg gcatcgcagg
      181 agcaaacccg gagctattgg atacaacggc tgagcggtat cctatggctc tccgccgcca
      241 cgttactgct tggcggcatg ctggtggccg ctcttaacgt gttcaacacc atggattaca
      301 aggcgcagac tgaaacagca aggacgcaaa cgcgtttaca ggaggacatg tacaacgagg
      361 tggccaggaa tttccccagc accccgctga atggagaaaa cctgagaatt gcagtggaaa
      421 tgtcggatac gatcgctgcc aatgcaaaaa ctccgctgcg cttaatgcag gccgtctcag
      481 cttcactgga caatgccccc gagattctat tgcagcgttt gcgctggaag ctgaccacag
      541 acatcaacag caaggatgat gctgtccagt cacaagccca ggctgcccag ggtgtctcgg
      601 aatcggggac actttacgaa acaggttttc tggatggtga aatcagaaac ttccaggggg
      661 actaccgcgc agcgctgaat agcgtcaacc ggcttgccga cctgatgcgc aaggatcccc
      721 gggtcgggca ggtgcaggtg gtgcagcagc cagtcaacgt cagctctcaa accagtttgc
      781 agggcagcac gctggatgcc gccgcgcagc aaatgccggc agcccagttc aagcttaaag
      841 tcattcttaa gccggaagtc acgcaaccat gaaactgaac gctaaggact ggcaaacact
      901 gcaaccaccc ctgctggcgc tggctgcagt gctgctcgtc gtgttcctgc tcaactggta
      961 taccattcgc cagcaagatc tggcattcca ggccatgcag gcacagcagt cgcaactcga
     1021 gcaggcgcgc cggcattatc aggaatccgg cgccgaaaaa gaagccatca tcaagtatct
     1081 gccgcattac caggatctga tcgatcgtgg tttcgttggc gaggaacggc gcatcgaatg
     1141 gatcgaccat ctgcgcaacg tcaaccagca atacaagctg ttcggcatta cctacagcat
     1201 tggcagccag gagaagtaca agcccagctt cagtctcaat agcggacctt ttgaattgca
     1261 ccgctccgcc atgaagattg aatccgccct gctgcacgaa ggcgatttgc tgaccattat
     1321 caacgcgatg tcggcggcca accaagcccc tttcacgcta cgcgactgta ctatcaccag
     1381 gattgccact gccgccagga acaagttcct gcccaatttg aacgccagct gcgaaattga
     1441 ctggctgacg attaccgagc cgcagaaaac gggagtgacg ccatgactcc gttacgtcat
     1501 gtcatgtttc tggcatgcct gatcccggcg ctggcgtccg ccgatgacca gtttggccgg
     1561 ctgttcatga cccctgccga acgtaacaat ctgaattatc tgcgccagac cagcaggccg
     1621 ccggaaaaaa ttgtcgcggc agaacaacca ccagatgaag gtaccgaaaa agctgttgcc
     1681 acgcccgtcg ccccctctgc cgtcaccatg caaggctacg tcaaacgcag cgatggcaag
     1741 gccacggtct gggtcaaccg ccagccggta caggaaacct ccaggcatgg cgaaatcgaa
     1801 gtcggcaagc ttggcgcatc gggtgaccgt gtgcgtgtca aggtgcccgg tactggccag
     1861 acggtcgagt tgaaagccgg gcaaacctac gatcctgcaa gcggcaaggt ggtggagaat
     1921 gctcgcgagt tgtccaacgc cgaagccatg tcatctgggg cgcctccagc aaaaaagagt
     1981 gcggccgaag atagtctggc cgggaaagaa gaccagcatc ctcaggtaac agaggtgcca
     2041 tccatgcctt cggccagtaa aaaaccgtaa tggccgcctg gcgccgagat cagcacccag
     2101 ggcagcaagg catggtgctg atcatgctgt tgttcattgt cgggctggtg gctacggtct
     2161 atctagttca tgcgctggat ggcaatgcgg tcaaaatcga gcgggatcaa aagacggcag
     2221 agtctctggc cttggcgaaa gctgcaatta tcggatgggc agcaacacga accacgctta
     2281 cgcagttagg ccaattgcca tgcccggaag atacaacatt gatcggcctg cctactgaag
     2341 gaaatgccaa gagcgcatgt acgctgccgg cagtaggccg attgccctgg cggtctttag
     2401 ggctcggcga attaagagat ggcaatggcg ataacttgtg gtacgcgatc tctcctggtt
     2461 tccgcagcac cacgatcaat agcgacacgc cggcacaatt gactgttgat ggggtggctg
     2521 gcagtgcgat agccatagtt ttttctcccg gcaccatttt ggccgggcag acgcgcccga
     2581 tccccacaat tgccaatccg cctgatgtaa ctcagtatct tgatctatct aataacgacg
     2641 gcgatagtat ttttgtaacg tcgggtgcag cgggtagttt caacgataga ttactgacga
     2701 tatcccacaa cgagttgttt gctgtggtag aaaagcgggt agcaagcgag gtgttgaagt
     2761 gtctggatgt ttatgcttcc gcaccagtgg cggtgccgcc accggtaccg cctacaggcg
     2821 cttacccttg gcctgccatg cttgatacag tgatgccccc aacctatgtt ggtactgtaa
     2881 ataatttctt tggcagggtt ccagatagcc caatgggagg taattggtca ggcgcctgcg
     2941 caattccggt aggcggtact ggttggtggt tgaattggaa ggaaatggta ttttttgcat
     3001 tggcagatgg gtacaagcct actattgcta ctccagggtg cggtgcatgc ctcacggtat
     3061 tgccgccaac agccaccgca gataagcggg ttgttgtgct ggtcgcaggc cagaagctgg
     3121 ccggacaaat gcgatctact cccgctgaca agggaacatt aagcaattat ctggaaaccc
     3181 cgaatagtac aggaggggtt tcatttgcca aacaaaatat gacttctgga ttcaacgatg
     3241 tcgtggctta tcactgacat tacaggtgca aataaaacat gaaggatagc caaggtttta
     3301 gtttggtcga attggccgta gtgctggtca ttgttggctt gttgctgggc ggtttgctga
     3361 tgccactttc ggcgcagatg gatgtgcgtg ggtaccagga caccagcaag cgactggaag
     3421 aagccaagga agccattatt ggttttgcca tggccaatgg tcgattgccg tgcccagcca
     3481 gcagcacttc cttggggatt gaagacccgg ggggcggtgg tgactgcaac cacgattacg
     3541 atgggttttt accagctgcc acgcttggcc tgagcaacgt cgatgggggc ggttacgcta
     3601 tggacggatg gagtggcaac tctgtcaatc gcattcgtta tgcggtgacc acggccaacg
     3661 cgagtgcctt taccacgcct gggggaatgc gcggcatcgg tatggcgaca ctggcacccg
     3721 ccctgcgtgt gtgtacatcg ggcagcggag tgacagccac tacctgcggt acagccacat
     3781 cactgacgac tacggcggtg gccgtcattt tttctgccgg gaagaatgcg tctactgggg
     3841 gtactggtac ggatgaaagc aagaactacg atgatgatca ggtgtttgtc agccacgaac
     3901 cggtgcccgg cgccaatgaa ttcgacgata gtgtgacctg gatatctcct aatttgttat
     3961 acagtcgcat ggttgctgcc ggccagctgc cttaaatagg gccccatgaa cgaccaccag
     4021 ctgctccgtt acagccgcca catcctgctg ccgcagatcg cctacgaggg acaggaaaag
     4081 ctggcgcaat cccatgcctt gatcgtcggc gccggcgggc tggggtcgcc ggtggtgctg
     4141 tatctggggg cgggcggggt aggaaagttg accatctgtg attacgacga tgtcgatttt
     4201 tccaacctgc agcggcaggt gatccatacc acccagtcgg tcggcgtcaa caaggcgctg
     4261 tcagcgcagc aggccctggc ggtgatcaat ccggaaatca cggtggtgcc cattccgcgc
     4321 aaatccaccg aggatgaatt caccgagctg gcgcgcgatg ccgacgtggt gatcgactgc
     4381 agcgataact tccagacccg ctacgcgctc aacagaatct gcgtgcagtt gaaaaagccg
     4441 ctggtgtccg gcgctgcaat cggcttcgag gggcaggtga gcgtgttcga catgcgccat
     4501 gccgatagcc cgtgctacca ctgcctgttt ccggacgtgg gcgccgagga tggggtgggc
     4561 gagatgcgct gtgcggaatt cggcgtgttc gcaccgctgg tcggcatgat cggcaccacc
     4621 caggcggctg aggcgatgaa gctgctgctg ggcgtgggcg aaagcctgca ggggcggctc
     4681 ttgttgctgg atgtgctggc gatggagtgg cgcagcatcc agctgaaaag agatccggct
     4741 tgcggcgttt gcgcctgaga agcggtcttt tgagaggcta gcgtttttcc cggatatacc
     4801 ggttgttgat gcgaaccgcg agcgcgccga cggcgatcag caggaatccg gccagcatca
     4861 ggccgactgg tccggcgagg ttgtgggcaa aatgctcggc catgtatttg ccgatatagc
     4921 cgatgagggc cagcgtgccg atgagcagca gcgtgcggga gcgtgccacg gtgctcagga
     4981 agatcgtgcc gcaggccagc gctaggaaca ggatttggta tgggcgatgg tccagccagt
     5041 cgtaggctgt ggccagaaac aggatggcgc cgacgaaata taccaatgcc gccaccggct
     5101 tgtgccgcga cttgtcgatg ctccagccga tgctcagcag ggaaaggccg atggtcaggc
     5161 cgatgaggtg ccgctcgaca tgcagcaggt cgaaggtgat gacaaagaag ccgactgaaa
     5221 agtaaaccgc cgtgaccgct agtacgctgc gccgcctggc gtaaaaggcg cagccttgct
     5281 ggatagccat taccaggttc aggaacagca agccgtaggc cggatcgcca ccgcgcgaga
     5341 actcatgcag ggtgaccagg atgccggtgg gttccagcaa tgccgcgacg agaaatagcg
     5401 gcgttgccgc tttctcgaag cggtcgtccg tagtgcagac cagcgccagg acaaagacgc
     5461 tgaagccggt gcctagcgtc agcagaatgc ggctggccgg gcttaggcta ctccactgca
     5521 tcccgatgta aatcgccagc ccggcaaaca cgaatatgcc gccgatatag ccgaacagcc
     5581 gggacagaat tccgctcgat cgctctgcct tgatctccag gccgcctttc agcgcctcgg
     5641 cgatctccac caggctgagg ttgtggcgct tgatgatatc gaccacctcg atgatggcgt
     5701 cctgccttgt gctcatttat ccatgaccca gccgatgaat tcgatgttgc cgtaataaaa
     5761 gttggtgcgc gcgttgccgg atacgagcct gatcgcactg ccgcccttcc gcagttgcgg
     5821 ttcgctgcta taccgattgc ccatgaacag ttcaccgacc agcccgctgt ggctatatcc
     5881 gtcgtaggag aggttgtagc cgtcggggga ttcggaagtg gtgtcgagtt tcagctcttt
     5941 ggtcgcctcc acgatttctt ctttcacatc gacaattttt tccatgtcgg cgggataggg
     6001 gaaggggagt tcgcgtacct tttgcgtctt ggcctggtaa atataaagct tcttccacgc
     6061 gttgtagagg tggccttcct tgcggttggg cgtgtactgc gccttcaggg tgccgtcctt
     6121 gtcgaccgtg agcttgaggg cgatcggcag gttgttgctg ccaacgtcag ccacggcaaa
     6181 aacaaggtcg tatttgggtg ggtcggtcac gatgttgggc aggctgcccg cgatcatgaa
     6241 gccgaacatc agcagcacag gcagggcaag tccgacgatc agcacgaaat tgtttttgac
     6301 gaaatccttg aaagccatgc gtcatccctt cccttggtaa aagcgaatcc ccgtcgagcg
     6361 gggttgattt gcctgtgcgc tatttcgacc caagcaggct tttcaccgcc ccaacgaggt
     6421 cgccgggcag cagcaccagt ttgctgtttt ccgaggtaga gagtttctgc atggcctgga
     6481 tgtagcggtc gccgagcagg aaggtggcgg aggcgttgct gtccttgacc gcttcgccga
     6541 tgaggcgcat ggcctccgcg ctggctttgg ccgccacctc ctgcgcctcc gcgtcgcgcc
     6601 gcgcggcttc caggcgcgct tcggcgttga gaataatggc ctgcttctcg ccttccgagc
     6661 gggtcaccag cgccacccgt tcgcgttcgg ccgaggcctg catttccatt gccttctgca
     6721 tgttgggcga aggcttgatg tcctggattt cgactgactt gaccgtcagg ccccagtcga
     6781 tggcttcgtc ggcgatggca gtcttcagtt ccgccttgat acggtcgcgc gaggacaggg
     6841 cggagttcag gtccatgccg ccgatgatgg agcgcaggct ggtctgtacc atattgcgca
     6901 tggcctcgcg gaagttctcg atgccgtaga ccgcacgctc gatattgctg accttgagga
     6961 aggcgatggc gttggccagg atgacggcgt tgtcgcgggt aatgacttcc tgctccggga
     7021 tgtccaggat gatgtccttg gtggtgacct tgtagcgcac ctggtcgatg aacgggacga
     7081 tgatgctgag gccgggattc aataccacgt ggaagcgccc gagacgttcc accacccatt
     7141 cctcgccttg cggcacgatg cgcaccccca tccagatgcc gacaacgacg gggaacagca
     7201 gaaccagcgt gaattcgaac attgatgact ccctaattgg cgcggttgac gcgcagatag
     7261 ttaccctcga tgccagacac cacggcggtc tcgccggctt cgatgcgttc atccgagacg
     7321 gctgtccatt cgcggctacc catcaccggc atggtgaagg tgatgcgccc caattgttct
     7381 ggtgccaccg tcgcgctgat gcgccctgcc tggccgatgg cgtcaccgtg ggactggcct
     7441 atccgcaggt cggccacctt tccgtggtac ttgagtttcc agactgtcag cgaagtcagg
     7501 gcgagtacag aaaacgccag cagttgcagc gaaaacggtg cgtctggcca gagcgccaga
     7561 agcagcgcca cacacagcgc cgcaataccg aaccacagga ggtagaacgt tccgctcagc
     7621 atttcggcgc cgagcagcac gaggccgaga atggcccaag cccatagcgg ttgcagcacg
     7681 atggaattcc ttgaagatga cgcttgaaat cctagcagaa attgctagag ttgcggggac
     7741 tgccgggttc cgcccggcgc ttcgtccaaa ggggatgcga tggtgagcgt gatcgttgta
     7801 gcggtgctgg tggtggcgct ggtgtatggg gtgatgcttt acaacaatct ggtgctgctc
     7861 aagaacaacg ttgccaaggc gtgggcgaat atcgacgtct tgctcaagca gcgccacgat
     7921 gaactgccca agctgatcga tacctgcaag cagtacatga aatacgagca ggaaaccctg
     7981 gaaaaggtca tccaggcgcg ctcccgcgtc gccgatgcgc gcgagtcgcg caacatcgcc
     8041 gccctcggcg cggcggaagg tgcgttgcgc gccgggctgg gcagcctgtt cgcgctggtg
     8101 gagtcttatc ccgagctcaa ggccaacgag caattcctgc atctgcaggg gcgcatcacc
     8161 ggcctggaga acgccattgc cgaccgtcgc gagttttaca acgagagcgc caatatcaac
     8221 aacgtgcgca ttgagcagtt tcccgacctg atcgtggcgc ggttgttcaa tttcagcgat
     8281 gccgagatgc tgcgtttcga agcggcagaa ctggaagacg tggacgttgc caggcgcttt
     8341 ggctaacaag tcatcctgac aatgcgctgg gcggaaaggg atgtgcgaag cattgaagtc
     8401 cggctattcc aacctgatca cttcctgcgg ccatctggtg ctcatactgg tggcgttgcg
     8461 catcaaccag ccgccggtct gggtggcctg cctgggcctg atttccgtca tcagcttcgt
     8521 tgcctgggct tccaatctca ggcgtaaccg cgccattgcc gacacgccca cttccagggt
     8581 ggcttctgct gcccaaggct atgtcgagct ttacgggccg gcagtcaggg gggcggagtt
     8641 cctggcccgg agccggctga gcggcatgcc ctgcgtatgg taccgccacg tcacctacca
     8701 gaagaattcc gatggcgaat gggcggagat cgcgcgcggc accagcgata cgctgttcgc
     8761 gctggaggat ggcagcggcc gctgcctgat cgacccggat catgccgaag tcatcacgac
     8821 ccattcccgt acctggatcg agggcgacta caagcacatc gaagagcagc tgttcgccag
     8881 cgacaatatt tatgccttgg gcgaattcac cacggtaggc ggcgccaatg cacatctcga
     8941 cctcaagcag gacgttgtgg ccatgctggc cgagtggaaa aaagaccagc tctcgctgct
     9001 gaagcgtttc gatctcaacc gcgacggaca gatcgacctg caggaatggg aacttgcccg
     9061 acgtgccgcc atccgcgaag tggaaaagca gcatcgcgat ctgcgcatgc agcccggggt
     9121 gcacgtcatg cgggcgccct ccggcggcca gatgtatctg ctttccaacc tgtcgccgca
     9181 gcagctgaag aatcgttatg tgcggtgggg ctggatacac ctgctgacgt tttttgcggc
     9241 ggctgccggg acggcctcca tcgggctggg ggccgcgctg tggtagaatc gcgccccatg
     9301 agcgaactcg acaaatcctt tgaacccagg aacatagaaa accgctggta ctccttctgg
     9361 gaagaccggg gctactttgc cgctggcctg gacacggaaa aatccgacaa cttctgcatc
     9421 ctgttgccgc cgcccaacgt caccggcacg ctgcacatgg ggcacggctt caaccagacc
     9481 ctgatggatg cgctgacgcg ctatcaccgc atgaagggcg acaacaccct gtggcagccg
     9541 ggtaccgacc acgctggcat cgccacgcag atcgtggtgg agcgccagct cgacgcccag
     9601 ggcctgtcgc gccatgacct cggccgcgag aaattcctgg aaaaagtgtg ggagtggaag
     9661 gagcattccg gctcgactat tacccgccag atgcgccgtc tgggcacctc gcccgactgg
     9721 agccgcgagc gcttcaccat ggacgccggg cttaacaagg tggttaccga aactttcgtg
     9781 cgtttgtaca acgaaggcct gatttaccgc ggcaagcggc tggtgaactg ggaccccaag
     9841 ctgcatacgg cggtttccga cctggaagtg gtgcaggagg aggaggacgg ttccctgtgg
     9901 cacatccgct acccgctggc ggatggctcg gaaagcctgg tggtggccac cacccgcccg
     9961 gaaaccatgc tgggcgacac cgccgtgatg gtgcatccgg aggacgagcg ttacgcgcac
    10021 ctgatcggta agacggtgaa gttgccactg accgagcgcg aaatccccat catcgccgac
    10081 gattacgtgg accgcgagtt cggcaccggt gtggtcaagg tgaccccggc gcacgacttc
    10141 aacgactacg ccgtgggcca gcgccaccag ttgccgatga tttccattct gaccctggat
    10201 gccagggtca acgagcacgc cccggagaaa taccgcggca tggatcgttt cgacgcgcgc
    10261 aaggtcatcg tcgccgatct cgaagcgctg ggcctgctgg tcaagatcga caagcacaag
    10321 ctcaaggtgc cgcgcggcga ccgtaccggc gtggtgatcg agccgatgtt gaccgaccag
    10381 tggttcgtcg ccatgagcaa gcccggcgcc aacggcaagt ccatcaccga gcaggcgctg
    10441 gaagtggtcg ccaacggcga aatcaagttc catccggaaa actgggtcaa cacctacaac
    10501 cagtggctga acaacatcca ggactggtgc atttcgcgcc agttgtggtg gggtcaccag
    10561 attccggctt ggtacggcgt caatggcgag gtgttcgtgg cccatgacga agccgaggcg
    10621 cgccatctag cggacgagga aggctatgct ggcccgctga cgcgtgacga ggacgtgctg
    10681 gacacttggt actcctccgc gctgtggccg ttctccaccc tggactggac gccggagtgg
    10741 ccggccaagt ccaacccggc gcttgatctc tacctgccgt cttcggtgct ggtcactggc
    10801 ttcgacatca tcttcttctg ggtggcgcgc atggtgatga tgacgaagca catcaccggc
    10861 aagattccgt tcagggatgt ttacgtgcac ggcctgatcc gcgacgcgga aggccagaaa
    10921 atgtccaagt ccaagggcaa tgtgcttgac ccgattgacc tgattgatgg tattaatctg
    10981 gacgatttgg tgaagaagcg caccaccggc ctgatgaacc ccaagcaggc ggagcagatc
    11041 gagaaacgca cgcgcaagga attcccggaa ggcatcccgg ccttcggcac cgatgctttg
    11101 cggttcacct tcgcatcgct cgccagccca ggccgtgaca tcaagttcga catgcaccgc
    11161 tgcgaaggct atcgcaattt ctgcaacaag ctgtggaacg ccacccgttt cgtgctgatg
    11221 aattgcgaag ggcaggactg cggcatggag ccttgttccc acgactgcgg gccggaaggt
    11281 tatctggact tctcgcaggc cgaccgctgg atcgtcagcc ttctgcagca caccgagacc
    11341 gaggtggaaa agggtttttc cgactaccgc ttcgactatg tggcgcaggc gatttacaag
    11401 ttcgtgtggg acgagtattg cgactggtat ctggaaattg ccaaggtaca actgcagaac
    11461 ggcagcgatg cgcaacagcg tgccacgcgc cgtaccttgc tgcgcgtgct ggaaaccgtg
    11521 ctacgcctgg cgcacccggt gattccattc atcactgaag aactgtggca gaccgtttcc
    11581 gaatataccg gccagcaggc cgacaccatc atggtgcgtc cgtatcccaa gagccagccg
    11641 gagaagatcg acgaggctgc cgaggcttgg gtggctatgt tgaagcaggc ggtggatgcc
    11701 tgccgcagcc tgcgtggcga gatgggcgtg tcgccggcca cccgtgtacc gctgattgcc
    11761 gccgggtcgg atgtgctgac agcctatgcc ccttacctga aggcattggc caagctggcc
    11821 gaggtcgagg tggtgaacga gttgccggag gccgatgcgc cggtggctgt ggtgggcgac
    11881 taccgcctga tgctcaagat cgaagtcgat gtggctgccg aaaaggagcg tctgggcaag
    11941 gaagtggcgc gtctggaagg cgaaatcgcc aaggcgaacg caaaactgtc caacgagagt
    12001 tttgtcgcgc gcgcaccggc agcggtggtg gcgcaggaga aggaacgtct ggccggcttc
    12061 atggcgatgc tggaaaaact gcaggcacaa ctggtacgtt tgaaataagc cgtatctgaa
    12121 atggaaacag cctcccgcgg gaggctgttt ttttatccgg ttacgctgat caaataaaca
    12181 ggctgacgtc tgcttctccg gcaaactcga agaaggtggc ggcaccgccg aattcgatat
    12241 cctcgatgaa gtcttccttc ttgaagtcga acaagtccac cgtcatctgg caggcgatca
    12301 gtttgacgcc cgcctccagg cacaggctgc gcaattcttc cagcgaggcc acgcccttgt
    12361 ccttcatttt ctgtttcatc atcatggtgg ccatgccttc catgccgggc agcatctgta
    12421 ccaggttggg catgggcacc ggcatcggca tcgccgggtt gccgagcggg ctgatccttg
    12481 catccagttc cttcttcaac agcgtgaggc cgtagaaggt gaaaaaaatt tgcacttcgt
    12541 aatccagcgc ggccgccgtc gaggcgagga tgaagggcgg gtaggcccag tccagcgtgc
    12601 ccttggaggc gatgagggca agtttcttgg acacggcttt aagccttttt cttcatgaag
    12661 taggtgtatt caccgttggc ctcgctggat tccagcagtt catggccggt ctgccggcag
    12721 aacgcctgga aatccttcac cgagccggag tcggtggcca cggtcttgag tacctggcca
    12781 ccggtcatgt ccgccagtgc tttcttgacg cgcagaatcg gcagcgggca gttcagcccg
    12841 cgcgcatcta cttccttgtc gaattccatc gatttgctcc tattgcttcg gttcaaaagg
    12901 gtggccagcc ttgccccaag cgatcacgcc accctgtaag ttgaaaatat tgtcgcgccc
    12961 cttgctggcc atgaaagcgc aggcttgtgc cgaacgtatg ccggcgtggc aatagaacac
    13021 cagcggttgc tcgcctccca gttcatcggc cttgaccggg atcagcgaca gcgggatgtg
    13081 gacggcgccc gggatgacgc ctctggcgac ttcttcattg ttgcgtacat cgaccagcag
    13141 tacgttatgc agaccgagca atgattgcag ataagttgcg tcgatatcct gatagcctga
    13201 cacgttttta gcccctgttg ccgcatgaaa aacggcgtga agtcatgtga tagataatta
    13261 tagcaggtag aaaagcgtgt ttaaggccgg caaaaagctt gcgctccgat catttgttcg
    13321 tttatgatga aagcgaacgt ttgttctgtt ttggaggccg tgatgggtga tgattccagc
    13381 tatctttcga cattgcagga ttactatgcc gagcagcgcg tcatgccttc gttcgcgaag
    13441 atttgccagc tgctcaagtt cagctcacgc gcttccgctg cggcgctggt tgccaggctc
    13501 aagctgcaag gcttcctgga agccaccgaa accggccggc tcaaaccggg taaacgcttc
    13561 ttcgagcgta tcctggctga cgacgtggtg cgcgccggca tgcccagccc agcgtatagc
    13621 ggcagcagcg acaccctcac catcgatgaa tacctggtgg accagccctc caaaaccctc
    13681 ctcatcccgg tcaagggtga ttctatggtc gacgccggca tcctgccgga cgatatcgtg
    13741 gtagtggaaa agcgtcagct cgccagcgtt ggtgacatcg tcgtggccat cgtcgacggc
    13801 gaattcaccc tgaagacgct gggccgcgaa aaaggcgagt tcgtgcttta cccggccaac
    13861 aaggcctatc cggtgattcg cccggaagga cagctggaga ttttcggcgt ggtggtaggg
    13921 cagttcagaa agtacggctg atcccaaacc aatgggaaac gatactaacg cctctcggcc
    13981 ttatttcagc gagaaatcga cccggttcgg cttgcccttg tccttgatgc cctcgctgtt
    14041 gagcttgggt ggtgtgatga acacccgttc cgcggcgacg ctgccggcct tggtgagcca
    14101 tgtgctgacg gcgtcggcgc gctgattggc cagccgacgg aggtcttcct caccgaccgt
    14161 gatgttggtc aggatcagct tttccatttc cgccggcggc aggtccttgg caaaaccaat
    14221 caggttgcgc ggcttgggaa acttttcttc cttgtaagcc tgcttgaggt aaaccggcca
    14281 ctcctcggcg ctgatggtga ccgagtcgac ggaagcggaa tcctcgccct tcttgactgt
    14341 attcttgagc ttctgcgcct tgaccttgtg ctgcagcccc gcttgcttga ggccttccct
    14401 gtcggtttct ggatcggcgc ggccggcgat atccagtttc agggcggggc gatcgttgag
    14461 cgccttggcc agcgtttgca atttttcctc ccccgtcttg gaaatggtcg ccaggcccgg
    14521 atcgaactcc agatactcca gttgctggtc gcccccacca aacagggagc ccagcaacgc
    14581 gaagggcgaa gtcacgatct tgacgatcag gttgccgatc attttccaga cgatgctgcc
    14641 gatgctgaat tgcgggtcgt cgagggatcc ctggatcggt agattgatgt cgatttcacc
    14701 cttgctgttc ttgagcagcg ctattgccag cgttaccggc agcttggtgg cgtccgggct
    14761 ttccactttt tgcccaaaag tgagttgatc gagaaacacg tggttttccg cttgcagctt
    14821 gcggtccttg atgaagtaat ggatgtccac cgaaagcttg cccttctcga tggcgtagcc
    14881 ggcatagcgc gtggcgtagg gattgatgcc gctgagttcg atgcccttgg cactggcctt
    14941 gagatcgaca aatatatcct tggccaaggg gttgatcttg ccggcaattt cgagtggggc
    15001 agtgccgttg accttgccgc gcaagtcgac gtcggccgtg cttcccggct ccgacgacaa
    15061 tccgctgacg ctgccgatga tttcggaaag gtcggccgaa tagttgggct tgatgaagaa
    15121 atcgctgaaa ttgatgtcgc cgccgctcaa ggtgaccttg tccagccgca gcttgaacgg
    15181 actgggctgt gcgggcgccg tggccaccgg tacggcactt tttccggcat cttgctgcgt
    15241 ctggcttgcg ttcgtctcgg ccgggggggc gctttcttcg tccgtcagca gctcgccggt
    15301 ccggctgcgg cccttgacca gcttgcgcag cgccagccgg ccatcctcgt tgaccacgat
    15361 gcgcgcatag aagtcattga gcgccagacc ggcaatggcg atgtcgtaag gggtttcgcc
    15421 ggtgttggcg ccgacttgca cgttgtccag atccagcgtt ttccatttca gcaagtcgga
    15481 ggaattgctc ttgtcgacca ggtggaattc
//
LOCUS       DAOMFA010000019        15181 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_323_length_15181_cov_5.182401, whole genome shotgun sequence.
ACCESSION   DAOMFA010000019 DAOMFA010000000
VERSION     DAOMFA010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 15181)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15181)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15181
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_323_length_15181_cov_5.182401"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1180)
                     /locus_tag="PKW44_01890"
     CDS             complement(<1..1180)
                     /locus_tag="PKW44_01890"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HQR50371.1"
                     /translation="MSFENLELHPAILHALQEAGYTEPTPIQSQAIPEIIAGHDLMAS
                     AQTGTGKTAGFMLPALNLLATPHPSRSRGPRILVLVPTRELAAQVNDAARKYGKFLRA
                     RTVSIVGGMPYPLQNKLLSQPLDILVATPGRLIDHMERGRIDFSRLQMLVLDEADRML
                     DMGFMPDVERICSAIPAERQTLLFSATLEGNIARIAAQILRNPKTIQVASQQAKHENI
                     EQRLHMVDDMGHKNKLLEHLLIAPDVKQTLVFTSTKRHADLLSEDLIAAGHKAAALHG
                     DMTQGARNRTLTKLRHGDISVLVATDVAARGIDVAGISHVINYDLPKFAEDYVHRIGR
                     TGRAGKSGIAISFASHAERFQLRKIEQFTGQRLEVAVIAGLEPKRIQQPKPHKFGDSG
                     K"
     gene            1658..2257
                     /locus_tag="PKW44_01895"
     CDS             1658..2257
                     /locus_tag="PKW44_01895"
                     /inference="COORDINATES: protein motif:HMM:NF028235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HQR50372.1"
                     /translation="MPSPSRDKLIAVARHLILERGFSAMSVNDVCLAAGVTKGSFFHH
                     FDSKETLGEVVLEEFWQGVQDRQENAAYRSAGTPVARLLGYIDHAIDTYQNPDLRSGC
                     LLAVYVSELRETHPGLYHKCIPHFLSWKSDVVSLLQDAVAQLSIKDQLEAEGWAEFYI
                     STLEGALMLAKALNDPQVITRVLTLYRNQLASLLSSGNP"
     gene            complement(2312..2929)
                     /locus_tag="PKW44_01900"
     CDS             complement(2312..2929)
                     /locus_tag="PKW44_01900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR50373.1"
                     /translation="MDMVVVFHKILHEHNFNIPFGMDTVRYGILAAEIVKEYFMKKIS
                     FLIGMAALMAGLAQAVAADDKPTDAMALKGATSGKVVWDINTSSPAKLALYLGVMQET
                     YEDLLRQGVTPDMVFTFRGESLRIISSEHDEITLQSMPEIRQSARIIHALMQRPGVRM
                     EACAIAARIFEVDIEKILHGIRPVGNTFVSLIGYQSKGYALIPIY"
     gene            2906..3709
                     /locus_tag="PKW44_01905"
     CDS             2906..3709
                     /locus_tag="PKW44_01905"
                     /inference="COORDINATES: protein motif:HMM:TIGR04557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein dehydrogenase-associated SoxYZ-like
                     carrier"
                     /protein_id="HQR50374.1"
                     /translation="MKNHDHIHLTLLALIAMMWMPSHAWAEAEDRWEILKDAFFAGKT
                     VENAPELIRLEAPKRAESGAQVPLSFSIEQPLTAEHYYKNVYILVGVNPVPLSGIFHF
                     TPASGKAEISTRIRLERDSYIHVVAEDNHGKLYMNAVPVRAAGGCGGTVEGDEKAIRA
                     EAGKMKLSVNAPVSLGGINRGKLLIRHPMYTGLQRDLVSQGFRPAFFANKATIKYHGK
                     TVLDADLFIGMSEDPNIQFNFLADTPGEMEVEVHDNEGGVFKNSIQVNG"
     gene            3714..4631
                     /locus_tag="PKW44_01910"
     CDS             3714..4631
                     /locus_tag="PKW44_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein relay system zinc metallohydrolase
                     2"
                     /protein_id="HQR50375.1"
                     /translation="MSALKIAWLVGLWGLAQGSALASPLPVQEVAQGIYVHQGAHEDF
                     DDNYHGDIANIGFIVGKDAVAVIDTGGYIKTGMALREAIRAVTDLPIRYVINTHVHPD
                     HIFGNAAFSADKPTYVGHHKLPAAMQQRAESYLRSLQNQFGKEAESTIMPPTLLVEDI
                     ATLDLGGRKLELKAWPTAHTNNDLSIRDTATNTLWLGDLLFIERTPSIDGDIKGWLKT
                     IKEIRTIDARLVIPGHGQVTSDKNAALDNQWRYLQTLLDDVRAAIKKGVSMSDAMNSA
                     AQSEARLWVLFDVVNRRNVNLLYPQLEWE"
     gene            4740..6551
                     /gene="typA"
                     /locus_tag="PKW44_01915"
     CDS             4740..6551
                     /gene="typA"
                     /locus_tag="PKW44_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HQR50376.1"
                     /translation="MSSRALRNIAIIAHVDHGKTTMVDKLLQQAGTFAAHQQVTERVM
                     DSNDLEKERGITILAKNTALNFEGTHINVVDTPGHADFGGEVERVLGMVDGVLLLVDA
                     VEGPMPQTRFVTKKALALGLKPIVVINKVDRPGARCNWVINQTFDLFANLGATDEQLD
                     FPVVYASALNGFATLDLEKSSDTMRPLLETVLKHVEPPRGDANAPLQLQISALDYSSY
                     TGRLGVGRIQNGRIKPGQVVVVMNGNDQIAQGRINQVLGFRGLERVAVEEAEAGDIVI
                     ISGLDDIGIGVTIADRDNPVGLPLMAVDEPTLTMDFMVNSSPLAGTEGKFVTSRQIRD
                     RLTRELLVNVALRVEDTKEADVFRVSGRGELHLTILLENMRREGFEIAVGKPRVVYKE
                     VDGAKCEPYEILTVDLEDDNQGAVMEELGRRRGELTNMASDGNGRTRLEYRIPARGLI
                     GFQSEFLTLTRGTGLMAHIFDDYAPVKADMPGRRNGVLISAEHGEAVAYALWKLEDRG
                     KMFVSPGDKLYEGMIIGIHSRDNDLVVNPIKGKQLTNVRASGTDEAVRLTTPIKLTLE
                     SAVEFIDDDELVEITPQSIRIRKRHLQEHERKKAARE"
     gene            6609..7697
                     /locus_tag="PKW44_01920"
     CDS             6609..7697
                     /locus_tag="PKW44_01920"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HQR50377.1"
                     /translation="MKPDFSRVPMVDVIKVVASQLIIWHHLAFYGPMSDVVYPHAPQM
                     IDWLYDNARIAVQAFLVIGGFLAARSLVPSLEAVWTDIRRLNVFSLIWRRYVRLARPY
                     LAALLIAMVAAALARQLIEHPAIPQMPSHQQIVAHIFLLQDLVDVEALSAGVWYVAID
                     FQLYALLVLVLWFSSRMADTTGMPASHLVLGMCLGLTGASLLWLNRNPGLDEWAPYFF
                     GAYGLGILAQWISQHPRKGRWVALLAVLVLAALALEWRSRTLVAGLIALLLILSVDGR
                     LPPRWLDNKPVAALGKISYSVFLIHYPVCLAVGAVVYRLWPSSVAINFLGMFAAWLLS
                     LGAGFILYQTVESSGFGRPSNLKGSVSE"
     gene            complement(7727..8026)
                     /locus_tag="PKW44_01925"
     CDS             complement(7727..8026)
                     /locus_tag="PKW44_01925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR50378.1"
                     /translation="MDKSKVKSLAIIFLLSILTVFPAFVSQVAARVVAVEGPRGDEAV
                     AVEGPRGNVAVGTRVDVLPDTASPVVVRGTQYYVDGGVYYKPDDSGAYVVVTAPE"
     gene            complement(8091..8966)
                     /locus_tag="PKW44_01930"
     CDS             complement(8091..8966)
                     /locus_tag="PKW44_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HQR50379.1"
                     /translation="MKTPALLSTGLLLAIALSAQAFDLDGALKSVQEATQPAPSTPTA
                     SDSTGTPLPPINLKKPSREDEIRLGREIAGNLLGAAPLVRDEKLQKYVNQVGRWVALQ
                     SERPDLPWHFGVIESDDLNAFAAPGGWVLVTKGLYRQLNNEAELAGVVGHEIGHVVKK
                     HQLKVIQKQQLLSYGAGLLGGTLGKDNQTVQQLIGSGAEICARSLDKDAEFEADRIGI
                     VLAGRAGYDPYGLVEVLQTIGHAGKDDSRVALLFKTHPHPDERLTKLGDAAGYRLDAI
                     KDGKVLTERFYLLKN"
     gene            complement(8963..9469)
                     /locus_tag="PKW44_01935"
     CDS             complement(8963..9469)
                     /locus_tag="PKW44_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HQR50380.1"
                     /translation="MKPRYLVIALLFAPCLALAEAGTALKDDTLRMEPYADAKSSGVL
                     KRSDKVDILEKKGAWLKVKAPKGSGWVRLLSVKRGEAGKSNEAAGVLSVASGRAGTGQ
                     VVSTTGVRGLNEEELKSAKFNEEEVKKLEANSISAEQAKQFAEAGSLKPQKLAYLPTP
                     KSGKGSGK"
     gene            complement(9466..11154)
                     /locus_tag="PKW44_01940"
     CDS             complement(9466..11154)
                     /locus_tag="PKW44_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HQR50381.1"
                     /translation="MLTRLALFISSLMSTVARRLQRNFYLYLAALLTLGVLLDASVFH
                     VGMNMRQKAFDLMVRSRVIAPKPDPDIVIVDVNEASLSALASEYGRWPWPRQVFGEFL
                     EQIEQQKPKAVVFDILFSDADVYNPDSDAYFNDTIAATGNTFFPFLRLPEAHDKLSQV
                     QPRMIPGIAETLPGKGKADATIAVVLPHFEAALVPGRLGTHNIYPDPDGIVREYPLWR
                     DDYGWKLPSLPLTIGGALHYPAPESQSVLLNWRGKPFSYRYVSFSDVYRDLTAKAPKR
                     LPNEFTGKIVIIGSTAPSLFDLKATAMAKAHPGVEILATAIDNVKHSDYLRFWRGALP
                     YVLMSLLLIWATASAFYRNVDRDRFNKLFSWSQIGLLVVSYIGINLTNTYLDFTGPVT
                     WAVLYFSVAKIYALATDRALQRRLALTTHTGAAGLHTALMPVQIESDEPLGDALLKKL
                     AREIEAVGSMPKSVEAIKGSQSGIWGLFGDMLVVGWTCAPNDAKQTGEIRREAAEIAG
                     RLNPLLQKIGLPDGITTRHVLHEGLIAGDKGLAPQWRILFAEAVIELEHHKDQA"
     gene            11293..12630
                     /locus_tag="PKW44_01945"
                     /pseudo
     CDS             11293..12630
                     /locus_tag="PKW44_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019898732.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
     gene            12634..12810
                     /locus_tag="PKW44_01950"
     CDS             12634..12810
                     /locus_tag="PKW44_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR50382.1"
                     /translation="MEDEGMMIVLIQQYAAKFGITFNSKAMDDEETKQKAYSLMMQAL
                     SGQRGPVTDEDLGL"
     gene            12807..13064
                     /gene="moaD"
                     /locus_tag="PKW44_01955"
     CDS             12807..13064
                     /gene="moaD"
                     /locus_tag="PKW44_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin converting factor subunit 1"
                     /protein_id="HQR50383.1"
                     /translation="MNLRILYFARLREALGVAKEEISVADGVADIQALMAWLAARGGV
                     WQQEFAGCRPLRAAVNQELVANSANFKTGDEIAFFPPVTGG"
     gene            13067..13525
                     /gene="moaE"
                     /locus_tag="PKW44_01960"
     CDS             13067..13525
                     /gene="moaE"
                     /locus_tag="PKW44_01960"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HQR50384.1"
                     /translation="MAVRIQHEDFDAGAEIARLRLARPDTGAVAAFVGQVRDINDGAS
                     VSGMTLEYYPGMTEKALEAIVEQARARWDIYDAVVIHRIGELKPLEQIVLVVVSGTHR
                     GEAFAACEFIMDYLKTEAPFWKKETTTEGARWVDARSSDDQAKLRWNTEN"
     gene            13530..>15181
                     /locus_tag="PKW44_01965"
     CDS             13530..>15181
                     /locus_tag="PKW44_01965"
                     /inference="COORDINATES: protein motif:HMM:NF025038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQR50385.1"
                     /translation="MPEALATHQLSLSLKAVDLGFADTSELLGGDCGWVGQARAEKAA
                     RFGLALDQPGYNLFVLGEVGSGRSSLLFRAMQEAAARRPPAPDLVYLYNFEQPERPLA
                     LRLSAGKGTLLRGGMDNFARNIARDIPRKLDEEGVHRDGERVRKACQERIDQAYVELN
                     AVAEARHFVLRREEGRLLFTLMGEDGQVMHEDTVLAMPAAQRAALEESEQELRVEIAR
                     YLEKVRPIEREMEQQRIAFRRATVEPVVQREVEAVRLALAGQVVDEARFATWLQRLSD
                     DVLDKLEIFSPSHADDDDAGIDALLSRYRVNVLVDNGGGAGAPALRDDDPVFRSLFGG
                     IEYQAENGVLVTDFMRIRAGDLLRAHGGFLMLHLRDLVRDPQGWEKLQRLLRSGRLQI
                     EEPAASFGQLATTTLEPEPVDVQVKLVLIGTREDYYDLQEADPEFASHFRVKVDFAES
                     FPANAETRRAAAVFIAQRCQALGLQHFTADAVARLLLFMQREIDDQHRMSAVFGRLES
                     LVVESAVFCRMRGADLISLQDVETALADRLLRHNYPEQVLLES"
BASE COUNT         3084 a         4245 c         4659 g         3193 t
ORIGIN      
        1 gttttccgct atcgccgaac ttgtgtggct tgggctgctg gatgcgcttg ggttccaggc
       61 cggcgatcac cgcgacttcc aggcgttggc cggtgaactg ttcgatcttg cgtagctgga
      121 aacgctcggc gtgcgaagca aatgagatcg cgatgccgct cttgccagcg cgaccggtgc
      181 ggccgatacg gtgtacgtaa tcttcggcaa acttgggcag gtcgtagttg atgacgtggc
      241 tgatgccggc gacatcgatg ccgcgcgcgg cgacgtcggt ggcaaccagc acgctgatgt
      301 cgccgtggcg cagcttggtc agggtgcggt tgcgcgcgcc ctgggtcatg tcgccatgca
      361 gcgcggcagc cttgtggccg gcggcgatca ggtcttccga cagcagatcg gcgtggcgct
      421 tggtggaggt aaacaccaaa gtttgcttga cgtccggagc gatcagcaaa tgctccagca
      481 gcttgttctt gtggcccatg tcgtcgacca tgtgcaggcg ctgctcgatg ttctcgtgtt
      541 tggcctgttg cgatgcgacc tggatagtct tcgggttacg caggatttgc gcggcgatgc
      601 gggcgatatt gccttccagc gtggccgaga acagcagggt ctggcgttct gccgggattg
      661 ccgagcagat gcgttccaca tccggcatga aacccatgtc cagcatgcgg tcggcttcgt
      721 ccagcaccag catttgcagg cgggagaagt cgatgcggcc gcgttccata tggtcgatca
      781 ggcgtccggg agtggcgacc agaatgtcca gcggctgcga cagcagtttg ttttgcagcg
      841 gatagggcat gccgcctacg atgctgacgg tgcgggcgcg caggaacttg ccgtacttgc
      901 gcgcagcgtc attgacctgt gccgccagtt cgcgcgtcgg gaccagcacc agaatgcgcg
      961 ggccgcggct gcgtgaagga tgcggcgttg ccagcaggtt gagcgcgggc agcatgaagc
     1021 cggcggtctt gccggtgccg gtttgcgccg aagccatcag gtcgtggccg gcaatgattt
     1081 ccggaatcgc ctgcgactgg atcggcgttg gttcggtgta accagcctct tgcagggcgt
     1141 ggaggatggc gggatgcagt tccagatttt cgaaagacaa aataattcct ttgttaaagc
     1201 gactgcgctc gccagacgtt ggcgtcgctt gtaacgcttt ggtaatcaaa atacgcaagc
     1261 agtcaccgtc ccgtcgtcac aacggaacag gcgcagaatt cggtgaggaa gtctttaccg
     1321 gcgctcgggc atagccacta accggtaaat ctttggaaga agctatgaag cgcgcttcag
     1381 aggggtcagg gcgatgaatc gtcggcacat ggccgaaacg aagcgcgcat tataaggaga
     1441 ttcgactgga aatcaagaat tttattcaaa caatggctat gcatttgcaa agctcatgag
     1501 ccaagaatgg gaaaatagaa caaacaggca ttgtggatac cccatgccgg atattgtgaa
     1561 atgttaatgt tcaaaattat cccggcagaa caaaacttgc gctgccgaac ggatttcatc
     1621 taggatgaaa cgaggttttt aattgagggc tgagaaattg ccatctccat cacgcgataa
     1681 gctgattgcg gtagcgcgcc atttaattct ggaacgcggg ttttctgcga tgtcggtgaa
     1741 cgatgtgtgt ttggccgccg gggtgaccaa gggaagtttc tttcatcact tcgatagcaa
     1801 ggaaactctg ggggaggttg tccttgagga gttctggcaa ggcgtccagg atcggcagga
     1861 aaatgcagcg taccgaagcg ccggtacgcc ggttgccaga ttgcttggtt atattgacca
     1921 cgcaattgat acctatcaga accctgatct cagaagcggt tgtttgttgg cggtgtatgt
     1981 ttccgagcta agggaaacgc atcctggctt gtaccataag tgcatccctc attttctctc
     2041 atggaaatcc gatgttgttt cattgttgca ggatgctgtc gcccaactat caatcaagga
     2101 tcaacttgaa gcggagggat gggccgagtt ctacatttcc acccttgaag gagcgctgat
     2161 gttggccaag gccctgaatg atcctcaggt cattacgcgt gtcctgacgc tgtatcgcaa
     2221 tcaactggcg tctcttcttt cttctggaaa cccctgaacc tttgtcagat ctgatggggg
     2281 actgccatca gatgactcgc cgcttctccg ttcaatatat cgggatgagc gcgtagccct
     2341 tgctttgata accgatcaac gatacgaaag tatttccgac cgggcgaatg ccatgcagta
     2401 ttttctcgat atccacctcg aatatcctcg ccgcaatcgc gcaggcttcc atgcgcacgc
     2461 cggggcgctg catcagcgca tgaattatgc gcgcggattg ccggatttcc ggcatggatt
     2521 gcaaggtaat ctcgtcgtgc tcggaggaaa taatcctgag gctctcgcca cggaatgtaa
     2581 ataccatgtc aggggtaact ccctgtcgca acaagtcctc ataggtttct tgcattacac
     2641 ccagatataa cgccagtttg gccgggctgg aggtgttgat atcccaaacc accttaccgg
     2701 aggttgcgcc cttcagtgcc atggcatccg ttggcttgtc gtcggcagcc acggcttgtg
     2761 ccaatccggc cattagtgca gccatcccaa tcaaaaacga gattttcttc atgaaatact
     2821 ccttgacaat ttcagcggca aggataccat accgaacggt atccatacca aaaggtatgt
     2881 taaaattgtg ttcatggaga atcttatgaa aaaccacgac catatccatt tgaccttgct
     2941 ggcactaata gcaatgatgt ggatgccctc gcatgcatgg gcggaagcgg aagacaggtg
     3001 ggaaatattg aaagatgcat tcttcgccgg gaaaactgtt gaaaatgcac cggaattaat
     3061 caggcttgag gcccccaaac gcgcagagag cggggcgcaa gtgccacttt cgttcagtat
     3121 cgagcaaccg ctgaccgcag aacattacta caaaaatgtt tatattctag taggtgtgaa
     3181 tccggtgccg ctgtccggca ttttccactt cacaccggca agcggcaaag ccgaaatttc
     3241 cacacgcatc cgcctggagc gtgactccta cattcatgtt gtcgcagaag acaatcacgg
     3301 aaagctatac atgaatgcag tgccggtgcg agctgccggt ggctgcgggg gaacggtgga
     3361 gggcgacgaa aaagctatcc gtgcagaagc cgggaagatg aaactatccg tcaatgctcc
     3421 tgtaagtctt ggcgggatca atcgggggaa actgctgatc aggcacccga tgtacaccgg
     3481 tttgcagcgt gatctggtgt cgcagggatt ccgcccagcg ttttttgcga ataaggccac
     3541 catcaaatac catgggaaaa ccgtgctgga tgccgaccta tttattggca tgagcgaaga
     3601 ccccaatatc cagttcaatt tcttagccga cacacccggc gaaatggaag tggaagtcca
     3661 tgacaatgag ggcggggttt tcaagaattc aatccaggtg aacggataac ttcatgagcg
     3721 ccctgaaaat tgcatggctg gtaggtctgt gggggttggc gcaaggcagc gcgctggcgt
     3781 ccccccttcc agtccaggaa gttgctcagg ggatatacgt gcatcaagga gcgcatgaag
     3841 acttcgatga caattatcat ggcgatattg ccaacatcgg ttttattgtt ggcaaggatg
     3901 ccgtggccgt cattgatact gggggataca tcaagacggg tatggcgctg cgcgaagcga
     3961 tccgcgccgt caccgatctg cctatccgct acgtcatcaa cacccacgtc catccggacc
     4021 acatcttcgg caatgcggct ttcagtgcgg acaagcccac ctacgtggga caccacaaat
     4081 tacccgcagc catgcagcag cgcgcggaga gctatcttcg aagcctgcaa aaccagttcg
     4141 gcaaggaggc ggaaagcaca atcatgccgc ccacgctgct ggtagaggat attgccacgc
     4201 tggacctcgg cggaaggaag ctcgaactga aagcatggcc aaccgcccat accaacaatg
     4261 acctgagcat ccgggacacc gcaaccaaca cattgtggct gggcgatttg ctgttcattg
     4321 agcgtacgcc ttcgattgac ggagacatca agggctggct caagaccatc aaggaaatcc
     4381 ggacgattga cgcaaggttg gtaatccccg gacacgggca agtcacttcg gataaaaatg
     4441 cagcgctgga caatcagtgg cgttatctgc aaaccctgct ggatgacgtc cgggccgcta
     4501 tcaaaaaagg cgtcagcatg agcgatgcca tgaactccgc cgcccagtcg gaagccaggc
     4561 tctgggtgtt attcgacgtg gtaaacaggc gaaacgtcaa tctcctttat ccgcaactgg
     4621 aatgggaata gtctctggaa tggatgggac agagcaggaa aattgaagta tcggggccac
     4681 tgaggctgat gtgttttatg ctagaatgcg cgactttttc tcagtaggct caattaatta
     4741 tgtcgtcgcg cgctctccgt aatatcgcca tcatcgccca cgttgaccac ggcaagacca
     4801 ccatggtgga caagctgttg caacaggctg gcacctttgc cgcccaccag caggtcaccg
     4861 agcgtgtgat ggattccaac gatctggaaa aagagcgcgg catcaccatt ctggccaaga
     4921 ataccgcact caatttcgaa ggcacccaca tcaacgtcgt cgacaccccg gggcacgccg
     4981 acttcggcgg tgaagtcgag cgcgtgctgg gcatggtgga tggtgtgctg ctgctggtgg
     5041 atgcggtgga aggcccgatg ccgcaaaccc gtttcgtcac caagaaggcg ctggcgcttg
     5101 ggctcaagcc catcgtcgtt atcaacaagg tggatcggcc cggcgcgcgc tgcaattggg
     5161 tgatcaacca gactttcgat ctctttgcca acttgggcgc gaccgacgaa caactggact
     5221 tcccggtggt gtacgcttcg gcgctcaacg ggtttgccac gctggatctg gaaaagtctt
     5281 ccgacaccat gcgccctttg ctggaaaccg tgctcaagca tgtggaaccg cccaggggcg
     5341 atgccaatgc gccgttgcag ctgcagatct cagctctgga ttattccagc tacaccggcc
     5401 gtctcggtgt cggccgcatc cagaatggcc gtatcaagcc gggccaggtg gtggtggtga
     5461 tgaacggcaa cgaccagatc gctcagggtc gtatcaacca ggtgctgggt ttccgtggac
     5521 tggaacgcgt ggcggtagaa gaagccgaag ccggcgatat tgtcatcatc tccggcctgg
     5581 acgatatcgg catcggtgtg accatagccg accgcgacaa tccggtgggc ctgccgctga
     5641 tggcggtgga cgagccgacc ttgaccatgg acttcatggt taactcctcg ccgctggctg
     5701 gcaccgaagg caagtttgtc accagccgcc agattcgcga ccgcctgacg cgcgaacttc
     5761 tggtcaacgt cgcgctgcgt gtcgaagaca ccaaggaagc cgatgtgttc cgcgtctccg
     5821 gtcgcgggga actgcatttg accatcctgc tggaaaacat gcgccgcgaa ggctttgaaa
     5881 tcgctgttgg caagccgcgc gtggtataca aggaagtcga tggtgccaag tgcgaacctt
     5941 atgaaatcct caccgtcgac ctggaagatg acaatcaggg cgcggtgatg gaagagctcg
     6001 gacgtcggcg tggcgagctg accaacatgg cctccgacgg caacggccgc acccgcctgg
     6061 aataccgtat tccggcgcgc ggcctgatcg gtttccagag tgaattcctc actctgacgc
     6121 gcggtaccgg gctgatggcg catatttttg acgattatgc accggtcaag gctgacatgc
     6181 cgggccgccg caacggcgtg ctgatttccg ccgaacacgg cgaggccgtg gcctacgcgc
     6241 tgtggaagct ggaagaccgc ggcaagatgt tcgtcagccc cggcgacaag ctgtacgagg
     6301 gcatgattat aggcatccac agccgcgaca atgatctggt ggtcaacccg atcaagggca
     6361 aacagctcac caacgtgcgc gcttccggta cggatgaagc cgtgcgcctg actacaccga
     6421 tcaagctcac gctggagtcc gccgtggagt tcattgatga cgacgaactg gtggaaatca
     6481 cgccgcagtc catccgcatc cgcaagcgcc atctgcagga acatgagcgc aagaaagcgg
     6541 cgcgcgagta agtgatccat agccgctaac gtgctttccg catgccggcc atgcaaacag
     6601 atatattaat gaagcctgat ttctcacgtg ttccgatggt cgatgtgatc aaggtggtcg
     6661 cgtcgcagct cattatctgg caccacctgg ctttttacgg gccgatgtcg gatgtcgtct
     6721 acccgcacgc cccgcagatg atcgactggc tttacgacaa cgcgcgcatc gcggtgcagg
     6781 cgtttctcgt catcggcggc tttctggcgg ccaggtcgct ggtccccagc ctggaagcgg
     6841 tctggacgga tatccggcgc ctcaacgtat tttcattgat ctggcgccgc tatgtgcggc
     6901 tggcgcgtcc ttatctggct gcactgctga tcgctatggt cgccgcggca ctcgcccgcc
     6961 agttgatcga gcaccccgcc attccacaaa tgccgtcgca ccagcagatc gtggcgcata
     7021 tcttcctgtt gcaggatctc gtggatgttg aggcgctttc tgccggcgtg tggtatgtgg
     7081 cgatcgattt ccagttgtat gcgctactgg tgctggtgct gtggttttcc agcaggatgg
     7141 ccgataccac cggcatgccc gcttcgcacc tggtactggg aatgtgcttg ggtctgaccg
     7201 gcgcttccct gctgtggctc aaccgcaatc ctggcctgga cgaatgggcg ccttacttct
     7261 ttggcgccta cggtctcggt attctggcgc aatggatatc ccagcatccg cgcaaggggc
     7321 gctgggtggc gctgctggcc gtgctggtgt tggccgccct ggcgctggaa tggcgcagcc
     7381 gtacgctggt ggccggcctt atcgcgttgc tgctcatctt aagcgtggat ggcaggctgc
     7441 ccccacgttg gctggacaac aagccggtgg ctgccttggg aaaaatctcc tactcggtgt
     7501 tcctgatcca ttacccggtc tgcctggcgg tcggagcagt ggtatacagg ctgtggccaa
     7561 gcagcgtcgc catcaacttc ctcggcatgt tcgccgcttg gctgctcagc ctcggcgcag
     7621 gattcatcct ctaccaaacg gttgaaagtt ccgggtttgg ccggccaagc aatctaaagg
     7681 gttccgtgtc ggagtagttt gagccggccg atcagataaa agctgtttat tctggcgctg
     7741 tcacgaccac ataagccccg ctgtcatctg gcttgtaata gacgccgccg tcgacataat
     7801 actgcgtgcc cctcactacg acgggcgagg cggtgtccgg caggacatcc accctcgtac
     7861 ctaccgccac gttgccgcgc ggcccttcaa ccgcaacagc ctcatccccg cgtggccctt
     7921 cgacggctac aactcttgcc gcaacctgtg acacgaatgc cggaaaaacc gtgagtatcg
     7981 agagcaggaa aatgattgct aaggatttga cttttgactt gtccatcatt gatctccata
     8041 ttcaatgtta gcgggtgtga ctttcatgaa tttccaatta tccatgaata tcaattcttg
     8101 agaaggtaaa accgttcagt cagcactttt ccgtccttga ttgcgtctaa ccggtagcca
     8161 gcggcgtcac ccagcttggt gagacgctca tccgggtgag ggtgggtctt gaacagcagc
     8221 gccacgcggc tgtcatcctt gccggcgtgg ccgatggtct gtagcacctc caccaggccg
     8281 tagggatcgt agccggcgcg gccggccagg actataccta ttctgtcagc ttcgaactcg
     8341 gcgtccttgt ccagactgcg ggcgcagatt tcggcaccgc tgccgatcag ctgctgcacg
     8401 gtctggttgt ccttgcccag ggtgccgcct agcaggccgg cgccgtagct gagcagctgc
     8461 tgcttctgga tgaccttcag ttggtgcttc ttgaccacgt gtccgatctc gtggccgact
     8521 acgccggcca gttcggcctc gttgttgagc tggcggtaca gtcctttggt cacgagcacc
     8581 cagccgccgg gcgcggcgaa ggcgttgagg tcatcggatt cgatcacgcc gaagtgccaa
     8641 ggcaggtcgg ggcgttcgct ctgcaaggct acccagcgcc cgacctggtt gacgtatttc
     8701 tgcagcttct cgtccctgac tagcggcgcg gcacccagca ggttgcctgc gatttcccgc
     8761 ccaaggcgga tttcatcctc acggctaggc tttttcaggt tgatgggcgg cagcggcgta
     8821 cccgtggaat cggatgccgt tggggtggac ggggcgggtt gcgtggcttc ctgcacgctt
     8881 ttcaatgcgc cgtcgaggtc gaatgcctgg gcagaaaggg cgatcgccag taacaggccg
     8941 gtggagagca gggcgggggt tttcatttgc cgctcccttt gccggatttc ggggtcggca
     9001 ggtaagccag cttttgcggc ttgaggctgc cggcttcggc aaactgtttg gcctgctccg
     9061 cgctgatgct gttggcttcg agttttttca cttcttcttc gttgaatttg gcgcttttca
     9121 gctcttcctc gttcaggccg cgcacgccag tggtggagac cacctggccg gtgccggcgc
     9181 gaccactggc cacactcaac acgccggccg cctcgttgga tttgcccgct tcgccgcgct
     9241 tgaccgacag caggcgcacc cagccactgc ccttgggtgc cttgaccttg agccaggcgc
     9301 ctttcttttc caggatgtcc accttgtcgc tgcgcttgag cacgccggat gatttggcat
     9361 cggcgtaagg ttccatgcgc agcgtgtcat ctttcagggc ggtgccggct tcggcgaggg
     9421 cgaggcaggg cgcgaatagt agcgctatga ctaaatatcg aggtttcatg cttgatcctt
     9481 gtgatgttcc agttcaatga cggcctcggc aaacagtatc cgccattgcg gggccagccc
     9541 cttgtcgccg gcgatgagtc cctcatgcaa cacatggcgt gtcgtgatgc catcaggcag
     9601 tccgattttc tgcagaagag ggttcagcct gccggcgatt tcggcagctt cccggcgaat
     9661 ctcgccggtc tgcttggcat cgttcggtgc acaagtccag ccgaccacca gcatgtcgcc
     9721 aaacaggccc cagatgccgc tctggctgcc cttgatcgcc tctacacttt tgggcatgct
     9781 gccgacagct tctatttcac gcgccagttt tttgagtagc gcatcgccca atggttcatc
     9841 gctttcgatc tgcaccggca tcagcgcggt gtgcaggccg gccgcgccgg tgtgggtggt
     9901 cagcgccagc cggcgttgca gggcgcggtc ggtggccagc gcgtagatct tggcgacgct
     9961 gaaatagagc actgcccaag tgaccggacc ggtgaagtcg agataggtat tggtcaggtt
    10021 gatgccgata taggagacca ccagcaagcc gatctggctc cagctgaaca gcttgttgaa
    10081 acggtcgcga tcgacgttgc ggtagaacgc gctggcggtc gcccagatca gcagtaggct
    10141 catcaataca tagggtaggg cgccgcgcca gaagcgcaag tagtcgctgt gcttgacgtt
    10201 gtcgatggcg gtggcgagga tttctacacc ggggtgcgcc ttggccatgg cggtggcctt
    10261 gagatcaaac aggctgggcg cggtagagcc gatgatgacg atcttgccgg tgaattcgtt
    10321 cggtaggcgc ttgggtgcct tggctgtcag gtcgcggtag acgtcactga agctgacgta
    10381 gcggtagctg aaaggtttgc cgcgccagtt aagcagtacg ctctgcgact ccggggcggg
    10441 gtaatgcagc gcaccgccga tggtgagcgg tagcgaaggt aatttccagc cgtaatcatc
    10501 gcgccacagg ggatattcgc gcacgatgcc gtccggatct gggtagatgt tgtgcgtgcc
    10561 gagacggccg ggcaccagcg cggcctcgaa atgcggcagc accacggcaa tagtggcgtc
    10621 agccttgcct ttgccgggca aggtttcggc aattccgggg atcatgcgcg gctggacctg
    10681 actcagtttg tcgtgggctt caggcaggcg caggaacggg aaaaaggtgt tgccggtggc
    10741 ggcgatggtg tcgttgaagt aggcatcgct gtcgggattg tagacgtcgg cgtcgctgaa
    10801 caggatgtcg aacaccacgg ccttgggttt ttgctgctcg atctgctcca ggaattcgcc
    10861 gaagacttgt ctgggccatg gccagcgtcc gtactcgcta gccagcgccg agaggctggc
    10921 ttcgttgacg tccacgatga caatgtccgg gtccggtttg ggcgcgatca cccggctgcg
    10981 caccatcagg tcgaatgcct tctgccgcat gttcatgccg acgtggaaca cgctggcgtc
    11041 gagcagcacg cccaaggtca gtagcgcggc caggtacagg tagaagttgc gctgcaggcg
    11101 ccgcgccact gtcgacatca ggctggaaat gaacagggcg aggcgggtca gcatgggcag
    11161 ggcggcttcc tattgccgtg gctggaaaag ttgatctggc atgccgccga gtttggcaca
    11221 aagacggcac aaaatgaagc atctatcgcc cggctggcac cgaatcggat gcgctataat
    11281 cccgtcaata ccatgctcga ctacctcatt ctcgccctgc tttgtttcgc cttgctggcc
    11341 atcggcattc tggcgctgca actacgccgc cagcagcgag ccctccccga cctgcaggcg
    11401 caactggagg acaagcaccg tgtcatgctt ctggacatgc acgacggcct caacaagctg
    11461 ggcgacaggc tgaatgcagc ttccggcgag tcatccgaac gcttacgcgg cgccgtcgcc
    11521 caggaactga cgcaaacccg tgacgccatg caggctttgt aactctcgca aatgcagaac
    11581 ctggagaaga tgcgcgaaac cgtattggag aagctgcacc agaccttggc cgaacagggc
    11641 aaggcggaac aggagctgat ccagtccacc atgcgcaatg ctacgctgca actcgccggc
    11701 acgatcgaag ccctgaccaa aaccgtcgat gcccgcctgc aggagatcag cggcaaggtc
    11761 accgagcgtc tcgatgaagg attcaagaaa accaatgaaa cctttgccag cgtcatggcg
    11821 cgccttgcca ccatcgacga ggcgcagaag aagatcgatg ggctgaccag caatgtcgtc
    11881 agcctgcagg aactgctggg tgacaagcgc tcgcgcggtg ctttcggtga ggtgcagctg
    11941 gaggcgttgg tgcgcaatat actgccggtc acggcctttg aaatgcagta tagtctgccc
    12001 aacggcacgc gtgtggattg cgcgctgttt ctcccggaac ccacgggcac ggtggcggtg
    12061 gattctaaat tcccgctgga aaactaccat cgcatgttcg agcacggact gcccgatatc
    12121 gagcgggtgc tggcaaaaaa acagttcaag gtcgacgtca agaaacatgt cgacgatatt
    12181 gctgccaaat acatcattcc caacgtcacc tccgatgggg ctgtgatgtt cgtgcccgcc
    12241 gaggccgtgt ttgccgagat ccacgcttac cactccgatg tggtggatta cgccatgagc
    12301 aagcgggtgt gggtggtgtc gccgaccacg ctgatggcgg tgctcaacac ggcgcgtgct
    12361 gtgctcaagg acgtggaaac gcgcaggcag gtacacatta tcaaggacga gctgggtaaa
    12421 ttagggaaag agttcggccg cttcgaccag cgcatgaaga aactggccga ccatatccgc
    12481 caagcgcatg aggatgctgt ggatgtgcac accaccagcc agaaaatcag ccgccgcttc
    12541 gctgccatcg aggcagtgga aatggaaaat gatctggcag cggtgcctgc cttgcccgtc
    12601 aacgacgaag acaaccgaac cgaggagtaa tctatggaag acgaaggcat gatgatcgtg
    12661 ctgatccagc aatatgccgc caaattcggc attaccttca attcgaaggc gatggacgac
    12721 gaggaaacca aacagaaagc ctactcgttg atgatgcagg ccctgagcgg ccagcgcggt
    12781 ccggtgacgg acgaggatct cgggctttga acctgcgcat cctgtatttc gcccgcttgc
    12841 gcgaggcgct gggtgtggcg aaggaagaga tttcggtggc ggatggcgtg gccgatatcc
    12901 aggcactgat ggcttggctg gcggcacgcg gcggggtgtg gcagcaggag ttcgccggct
    12961 gccgtccgtt gcgtgctgcg gttaaccagg agctggttgc caactcggcg aatttcaaga
    13021 ccggcgatga aatcgccttc ttcccgccgg tgacgggagg ctaagcgtgg ccgtcagaat
    13081 tcagcatgag gatttcgatg ctggcgccga gatcgcccgc ctgcgcttgg cacggccaga
    13141 caccggtgcg gtggcggcct ttgtcggcca agtgcgcgac attaatgatg gggccagtgt
    13201 ttccggtatg acgctggagt actaccctgg catgaccgag aaggcgctgg aagctatcgt
    13261 ggagcaggcc agggcgcgct gggatattta cgacgcagtg gtcattcacc gcatcggcga
    13321 actgaagccg ctcgagcaga tcgtgctggt ggtggtcagc ggtacacacc gcggcgaggc
    13381 ctttgctgcc tgcgagttca tcatggatta tcttaagacc gaagcgccgt tctggaaaaa
    13441 ggaaaccact acagaaggcg cacggtgggt ggatgcgcgc agcagcgacg atcaggcaaa
    13501 gctgcgctgg aatacggaaa actagactta tgccagaagc acttgccacg caccagctca
    13561 gcctatcgct gaaggccgtt gatctgggct ttgccgacac ctctgaattg cttggggggg
    13621 attgcggctg ggtcggccag gcgcgtgcgg aaaaggcggc acgctttggc ttggcgctgg
    13681 accagccggg ctacaacctg ttcgtgctgg gcgaagtagg cagcgggcgc tcttcactgc
    13741 tgttccgcgc catgcaggaa gccgctgcca ggcgtccacc cgcccccgat ctagtttacc
    13801 tttacaattt cgaacagccg gaacgccccc tggcattgcg gctcagtgca ggcaagggca
    13861 ccttgctgcg cggcggcatg gacaactttg cccgcaacat tgcccgcgac attccgcgca
    13921 agctggacga ggagggggtg caccgtgacg gcgagcgggt gcgcaaggcc tgccaggaaa
    13981 gaatcgacca ggcctatgtc gagctgaacg cggtcgccga agcccggcat ttcgtgctgc
    14041 gccgcgaaga aggcaggttg ctgtttaccc tgatgggcga agatggacag gtcatgcatg
    14101 aagataccgt gctggccatg cctgccgcac agcgtgcggc actggaggaa tcggagcagg
    14161 agctgcgcgt cgagatcgcc cgttatttgg aaaaagtgcg ccccatcgag cgcgagatgg
    14221 aacagcaacg gatcgctttt cgacgtgcca ccgtggagcc tgtagtgcaa cgcgaagtgg
    14281 aggctgtccg gctggccttg gccggacagg tggtggatga agccaggttt gcgacttggc
    14341 tgcaacgcct gtccgacgac gtgctggaca agctggaaat cttctctccc agccacgctg
    14401 atgacgatga cgccggcatc gatgcgcttc tgtcgcgcta tcgtgtcaac gtactggtag
    14461 acaacggggg gggcgctggt gccccggcgc tgcgcgacga cgacccggta ttccgttccc
    14521 tgttcggcgg tatcgaatat caggcagaaa acggcgtgct ggtcaccgat ttcatgcgca
    14581 tccgcgccgg cgatctgctg cgcgcccacg gcggtttcct catgttgcac ctgcgcgatc
    14641 tggtgcgcga cccccagggt tgggaaaagc tgcaacgcct gctgcgcagc gggcgcctgc
    14701 agattgagga gccggccgcc tcttttggcc agctggccac caccacgctg gaacccgaac
    14761 ccgtcgatgt gcaggtcaag ctggtgctga ttggcacacg cgaggactat tacgacctgc
    14821 aggaagccga ccccgaattt gccagccatt tccgcgtgaa agtggatttt gccgaaagct
    14881 ttcccgccaa tgcggaaacc cgtcgcgctg cggcggtgtt catcgcgcaa cgctgccagg
    14941 cattgggtct gcagcacttt acggcggacg ctgtggcgcg gctgctgctg ttcatgcagc
    15001 gagagatcga cgaccagcat cgcatgagcg ccgtattcgg gcgtctggag tctctagtgg
    15061 tcgaatctgc ggtgttttgc cggatgcgtg gcgctgacct gatcagcctc caggatgtcg
    15121 agacggcgct ggctgatagg ctgctacgcc acaattatcc ggaacaggtg ctgctggagt
    15181 c
//
LOCUS       DAOMFA010000020        14955 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_335_length_14955_cov_4.910403, whole genome shotgun sequence.
ACCESSION   DAOMFA010000020 DAOMFA010000000
VERSION     DAOMFA010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 14955)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14955)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14955
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_335_length_14955_cov_4.910403"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /locus_tag="PKW44_01970"
     CDS             <1..366
                     /locus_tag="PKW44_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR50386.1"
                     /translation="PFVYAVFPPLLLLDIFASLYHAVCFPLLGIPKVKRSDYLVYDHH
                     HLAYLNALEKLNCLYCSYGNGLISYIKEIIARTEQYWCPIKHAQRILDAHSRYGYFTE
                     YGDAESFRRDLEKIRQFKD"
     gene            423..1250
                     /gene="dapD"
                     /locus_tag="PKW44_01975"
     CDS             423..1250
                     /gene="dapD"
                     /locus_tag="PKW44_01975"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HQR50387.1"
                     /translation="MQQLQSIIEEAFDNRANITPANADKKIKNAVFGVLEELNAGRLR
                     VATRIGDSQQWETHQWIKKAVLLSFRLEDNALLDGGCTKYFDKVPSKFAGYTEADFKA
                     GGFRVVPNAVARRGSFIGKNVVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHL
                     SGGVGIGGVLEPVQAGPTIIGDNCFIGARSEVVEGVVVEDNSVISMGVYIGQSTKIYD
                     RETGEVTYGRVPAGSVVVSGNLPSKDGSYSLYCAVIVKKVDAKTLSKVGINELLRGV"
     gene            1250..1597
                     /locus_tag="PKW44_01980"
     CDS             1250..1597
                     /locus_tag="PKW44_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HQR50388.1"
                     /translation="MKLYGIPNCGTVKKARAFLEAHRIAYDFHDFKKSGVREESVDRW
                     LQQTGWQKLLKKTGPTWSQLPDAVKASINDDASALALMLDKPNVIKRPVLEQDGRVLA
                     TGFVEADYERLFK"
     gene            1594..2718
                     /gene="dapE"
                     /locus_tag="PKW44_01985"
     CDS             1594..2718
                     /gene="dapE"
                     /locus_tag="PKW44_01985"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HQR50389.1"
                     /translation="MSATLELSKQLIARRSLTPDDAGCQEILIERLEKLGFTVEKMRF
                     GNVDNFWARRGTAKPLVVFAGHTDVVPTGPVEQWLSEPFTPTIRAGMLYGRGAADMKT
                     SIAAFIIALEEFLARHPGHNGSIGLLITSDEEGVAVDGTVRVVEALKARGELLDYCIV
                     GEPTSNKQVGDMIKNGRRGSLSGKLVVKGIQGHIAYPHLVKNPIHKAAPAIAELAQTV
                     WDEGNEYFPPTSFQVSNIRGGTGATNVVPGHVEILFNFRYCTASTEQNLRERVLAILD
                     RHGLEYDLEWESGKPYLTPKGDLVAVISAAIEQAYGITPELSTSGGTSDGRFIADICP
                     QVIEFGPLNATIHKLNECVGVADIDPLKNTYRITLEKLLK"
     gene            2715..3614
                     /gene="prmB"
                     /locus_tag="PKW44_01990"
     CDS             2715..3614
                     /gene="prmB"
                     /locus_tag="PKW44_01990"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HQR50390.1"
                     /translation="MSFHPRAAEQLFTLRDWLRFAVSRFEEAGIFFGHGTHNSYDEAV
                     WLILSALHLPHDTLENFLDAVVTEDERRVLAHLIERRITERIPTAYLVREAWLGDFKF
                     YVDERVIVPRSFIAELLREQLAPWVENPEEVVSVADICTGSGCLAILAAHAFPNANVD
                     AIDISDDALAVARRNISDYGLDEHVHPIKSDMLSSLMGKHYDIIISNPPYVDAPSMET
                     LPQEYRQEPGLALASGDDGLDHTHTLLHNAVNHLNPGGYLIVEIGHNRDALEAAYPDL
                     PFTWLEVSGGDQFVFMLTREQLI"
     gene            3706..4209
                     /locus_tag="PKW44_01995"
     CDS             3706..4209
                     /locus_tag="PKW44_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HQR50391.1"
                     /translation="MLQLKNLPDAKILEKFVARYPDAEVESVLPFLNLLRAGADLSDA
                     LDALLAQHGLLQGRWWVLILLMREDDLISTPSDLAEKAGVSRATMTGLIDGLERDGLV
                     QRLSDDSDRRKLTIRLTALGQAKLDDVMPSYYQKVKQLMRVLTQSQREDLVAHLKLLT
                     ANIEALR"
     gene            4263..4649
                     /locus_tag="PKW44_02000"
     CDS             4263..4649
                     /locus_tag="PKW44_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgcJ/EcaC family oxidoreductase"
                     /protein_id="HQR50392.1"
                     /translation="MTTSPHTTIASLNSTWNQTFNRGDAAALARLYAENATLSPGNGQ
                     TLLGRAEIEKLFQSFVDGGVHQHTIDIVSTGGDEQVMFQVAKWNAHGAAQDGNTPSFG
                     GILMSAFEKGADGQWLVRSHVWNMAG"
     gene            4675..5040
                     /locus_tag="PKW44_02005"
     CDS             4675..5040
                     /locus_tag="PKW44_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase family protein"
                     /protein_id="HQR50393.1"
                     /translation="MPFVTISILKGKSAEYIQAVADGINAAVIETMGFPDDDRYQVIH
                     QLAPECLQLQTREGDRVMMHLVMRAGRSNKSKQAFYKKVVENLANNPGIPPANVLITI
                     TENHDIDWSFRDGVAQFVV"
     gene            complement(5084..6103)
                     /locus_tag="PKW44_02010"
     CDS             complement(5084..6103)
                     /locus_tag="PKW44_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HQR50394.1"
                     /translation="MTRPFKQTRTPRPQPAANPDQPSRRLHKLLALAGLGSRRDMEEL
                     IASGRITINGKTAQVGAGVVPSDVVRLDSRPLHLPFEAELPRVLLYHKPEGEIVSQDD
                     PEKRATVFDKLPHVRSGRWVAIGRLDINTSGLLIFTTSGELANHFMHPRYEVEREYAV
                     RLMGELTDAQMLQLTQGVELEDGMAQFDKIQDRGGEGANHWYEVMLKEGRNREVRRLF
                     EALGLMVSRLMRVRFGPVNLPARLKRGQMLELPSKDVAGLLEWAGLAVPRSPQRTPKE
                     RESLGKTFTPKERKPSKRSELPQSKNSRAPSRSAPASKRRVRGDAMNPPAQQKRSDRN
                     RGRGG"
     gene            complement(6100..6645)
                     /gene="scpB"
                     /locus_tag="PKW44_02015"
     CDS             complement(6100..6645)
                     /gene="scpB"
                     /locus_tag="PKW44_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HQR50395.1"
                     /translation="MSSDIQQMKNVLEAALLTSRAPLSLDQLASLFEERMERATLRML
                     LDELKTDWNSRTMELAQVASGWRFQARAEYWPHLARLNPDRPQRYSRAVLETLAIIAY
                     RQPVTRGDIEEIRGVSVNPNIIKTLQERDWIDVVGHREVPGRPSLFATTRTFLDDLNL
                     RSLAELPPLEHFSQHEIPLDL"
     gene            complement(6635..7426)
                     /locus_tag="PKW44_02020"
     CDS             complement(6635..7426)
                     /locus_tag="PKW44_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020184056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HQR50396.1"
                     /translation="MLISSPHARIHGELLAELPQDLYIPPDALEVYLEAFEGPLDLLL
                     YLIRKHNLDILDIPMADLTRQYMAYVETMRERNLELAADYLLMSAMLIEIKSRMLLPK
                     PAEIEQEDDPRAELVRRLLEYESMKLAAHKLDELPQVGRELLVAQAYFEHIPQQQLPG
                     VSMEDLLMAWQEVLRRASHYVHHKVSREELSVREHMSLILRRLNAKPLLEFTALFEVG
                     EGVPKLVVCFLAILELAREGLVRLTQMHEDAAAFSPIYVQANDVL"
     gene            complement(7433..7621)
                     /locus_tag="PKW44_02025"
     CDS             complement(7433..7621)
                     /locus_tag="PKW44_02025"
                     /inference="COORDINATES: protein motif:HMM:NF019540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HQR50397.1"
                     /translation="MKRLDLIRHLQANACDLLREGGSHSIFVNRAKGKVSAVPRHREI
                     NEMLARKICKDLEIPFGN"
     gene            complement(7618..7842)
                     /locus_tag="PKW44_02030"
     CDS             complement(7618..7842)
                     /locus_tag="PKW44_02030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HQR50398.1"
                     /translation="MNYQLTAVFEKVPEGYIAFVEELPGANTQGDTLEEARANLAEAV
                     ELVLEANRVLAEESLAGHEVIREPLNLAVA"
     gene            complement(7902..9677)
                     /locus_tag="PKW44_02035"
     CDS             complement(7902..9677)
                     /locus_tag="PKW44_02035"
                     /inference="COORDINATES: protein motif:HMM:NF015210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HQR50399.1"
                     /translation="MEAHNRFLPEWFNRIRTRQITLPRFQRYVAWGHDNVSDLLTTVL
                     RGLPSGAALILEVGDKEKFKSRTMTDAPELGDRVTEQLLDGQQRLTALWRSLHDKYPD
                     RTYLIYFEDDPSDAGAEKQPVVEGQARWVKNGLRFPIWVDSPRECWNRGRIPLRLLVP
                     GANDTDVDSWVTAALPDDQKDSLQSFNKVKNTINGLRTKVREFNLPYLALPVTTNKEV
                     ALDVFVKMNTSSVKLTTYDVIVALVEGETGKSLHEHVDELADACPRAAEYADLPSLVL
                     DLEALRQDRVPSQAGYKGIDYPGMVGDWPMIIQSVKGLVEFLEEESIFDAQRLPSYTP
                     LPVIAALWEMLPTQPDQLGNARALLRKYLWRAFLTARYEQASSSGALQDFRALKKVLL
                     GQGTEKDVPVLNQESYPLPTADLILQAGWPKSKTILGRGVLALQLKCGAIDFADAKPA
                     TVASITSKTHPREYHHLFPDSLLSDAGVPAEQIFRAVNCALVTWRTNRTISNKEPLIY
                     LKERTDNSALGEDEIKRRLRSHLIPFDDFAVGYSDLNTEEQKAKLRSDFEVFTKKRAV
                     IVARAASLVCNGGMINADEAFEFDD"
     gene            complement(9716..10918)
                     /locus_tag="PKW44_02040"
     CDS             complement(9716..10918)
                     /locus_tag="PKW44_02040"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020184055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HQR50400.1"
                     /translation="MAVERVLSGMRPTGSLHLGHYHGVLKNWLKLQHEHECFFFVADW
                     HALTTHYDTPQIIEESVWEMVIDWLAAGVDPASATVFIQSRVPEHAELHTLLSMITPL
                     GWLERVPTYKDQQEKLSSKDLSTYGFLGYPLLQSADILIYRATQVPVGEDQVPHIKFT
                     REIARRYNHIYGREPGFEEKAEAAIKKLGNKKGALYEELRTRYQESGDDEALEAARAL
                     IHEQQGLSLGDRERLLGFLEGGGKMILVEPEAVLTPASKMPGLDGQKMSKSYNNTISL
                     REDPDVISKKIRTMPTDPARVRRTDAGDPAKCPVWQLHEVYSDEQRKDWVQQGCKGAS
                     IGCIECKQPVIDAVLAEIKPIQERAAQYAEDPTLVKNIVADGCEKARKLARETMRDVR
                     EAMGLSYS"
     gene            complement(10932..11573)
                     /locus_tag="PKW44_02045"
     CDS             complement(10932..11573)
                     /locus_tag="PKW44_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HQR50401.1"
                     /translation="MELSLIQKITVYALPVIFAITVHEAAHGYVARYFGDMTAAAAGR
                     ISFNPLRHIDPIGTVLVPLLTMMAGGILFGWAKPVPVDFGRLRNPKRDMLWVAAAGPA
                     SNLVMAILWALALKLAPAFSEAIATPLVLMAQAGIMINVVLMVLNLFPLPPLDGGRIA
                     VSLLPQPYAWKFSQIERYGFIILIVLLFTGILGKILGPFIYAVIGLLSVLFNF"
     gene            complement(11575..12198)
                     /locus_tag="PKW44_02050"
     CDS             complement(11575..12198)
                     /locus_tag="PKW44_02050"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HQR50402.1"
                     /translation="MAQYFSIHPDNPQPRLVHQAAEIIRNGGVVAYPTDSCYALGCHL
                     GDKEAQERIRAIRGVDEHHHFTLVCRNLAEIATYAKVDNSRFRLLKANTPGSYTFILQ
                     ASREVPRRLQHPRRSTIGLRVPDHAVTLALLEDLNEPLLSMTLTLPGWDAPREAWEIR
                     EELESRVDLVIDAGACSHEMTTVIDLTGAEPVLVRRGRGDPAPFGLE"
     gene            complement(12203..13054)
                     /locus_tag="PKW44_02055"
     CDS             complement(12203..13054)
                     /locus_tag="PKW44_02055"
                     /EC_number="3.1.3.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011635059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097657 - 3',5'-nucleotide bisphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-nucleoside bisphosphate phosphatase"
                     /protein_id="HQR50403.1"
                     /translation="MASPIDLHSHSVVSDGTLTPSELVEHAAARGVRVLALTDHDDVA
                     GLEEAAEAAVRHGIALVNGVEISVTWNGRTVHVVGLKISPTHIPLLQGLATIRQGRYQ
                     RAEGMAASLEQAGIHGSLDGAHAYATCGIISRTHFARFLVQQGYAKDVSAVFKKYLVK
                     GKPGYQEHRWAALEDAVGWITGSGGIAVLAHPGRYDLGRVNMLKLLAEFRELGGTGVE
                     VVTGSHTPEQYHHFARLSHQFGLLASLGSDYHGPGQTYTDMGRLPDLPANCTPVWHDW
                     PEMRLAA"
     gene            13141..13674
                     /locus_tag="PKW44_02060"
     CDS             13141..13674
                     /locus_tag="PKW44_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HQR50404.1"
                     /translation="MKFLFDLFPIILFFVVFKSVGIFAATATAIAATLAQIGWVKYRH
                     GKVDGMLIASGVIIVVFGGATLLLHDETFIKWKPTVLYWLFAVVLFSAELFWNKNLIR
                     SVMEQQISLPQAVWRTLNHAWAAFFAVLGLLNLYIAFNFATDTWVNFKLFGTTALMLV
                     FVVMQSLLLGKYVKDQE"
     gene            13674..13976
                     /locus_tag="PKW44_02065"
     CDS             13674..13976
                     /locus_tag="PKW44_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HQR50405.1"
                     /translation="MLYAIIAEDAPNSLEKRLANRPPHLERLQQLQQEGRLLLAGPFP
                     AIDSVDPGPAGFSGSLIVAEFSSLEDAQAWAEADPFVAGGVYAKVTVKPFRKTLPA"
     gene            13976..14236
                     /locus_tag="PKW44_02070"
     CDS             13976..14236
                     /locus_tag="PKW44_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018509405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HQR50406.1"
                     /translation="MALPDEIRERLAILEPLQLEIEDDSARHAGHAGNQGGGHFNITI
                     VSSHFLQKSQIMRHRIVYQALNDLIPSRIHALSIRALTPDEI"
     gene            14271..>14955
                     /locus_tag="PKW44_02075"
     CDS             14271..>14955
                     /locus_tag="PKW44_02075"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HQR50407.1"
                     /translation="MTKRILVALALTGLLAPAVHAADPATVNGKPIKQSLVDFVLKDA
                     AAQGQKIDDQSKSLVLNRLVTQEVVIQEAQKAGLDKQADYLARQELANRELLINTYLQ
                     DFIKKNPISEADIKAEYDKFKQQLGDKEYNARHILVKTEQEAKDIIAQLAKGADFAKL
                     AREKSQDPGSKDKGGELDWFSPARMVKPFSDAVVGMKKGEDTKTPVQNQYGWHVIKLV
                     DVRKTQDPAY"
BASE COUNT         3213 a         4513 c         4160 g         3069 t
ORIGIN      
        1 cctttcgttt atgctgtgtt tcccccgctg ctgctgctgg atattttcgc cagcctgtat
       61 catgcagtct gcttcccgct gcttggcatc ccaaaagtca agcgcagcga ttatctggta
      121 tatgaccatc atcacctggc ctatctcaat gcgctggaga agctgaattg tctttattgt
      181 tcctatggca acggcctgat ttcctatatc aaggaaatca ttgcccgcac cgaacaatac
      241 tggtgcccga tcaaacacgc acaacgcatc ctcgacgccc actcgcgcta cggctacttc
      301 accgaatatg gtgacgcgga aagcttccgc cgcgaccttg agaaaatccg ccagttcaag
      361 gactgacgaa gttcccggaa ccaagtaaaa ttacgttttt ttcaaagcct taaggacccg
      421 ccatgcagca actgcaatcc attatcgaag aagccttcga caaccgtgcc aacatcaccc
      481 ccgccaatgc cgacaagaaa atcaagaatg ccgtgttcgg cgtactggag gaactcaatg
      541 ccggtcgttt gcgcgttgcc acccgcatcg gcgacagcca gcaatgggaa acccaccagt
      601 ggatcaagaa agccgtgctg ctgtctttcc gtctggaaga caatgcgttg ctggacggtg
      661 gctgcaccaa gtatttcgac aaagtgccga gcaagtttgc cggttacacc gaagccgact
      721 tcaaggccgg cggcttccgc gtggtcccca acgccgtggc gcgccgcggc tcattcatcg
      781 gcaagaacgt ggtactgatg ccctcctacg tcaacatcgg cgcctacgtg gatgaaggta
      841 ccatggtcga cacctgggcc accgtcggct cctgcgccca gatcggcaag aacgtccacc
      901 tctccggcgg cgtcggcatc ggcggggtgc tggaaccggt gcaggccggc cccaccatca
      961 tcggcgacaa ctgcttcatc ggcgcccgtt ccgaagtagt ggaaggcgtg gtggtggaag
     1021 acaactcggt gatttcgatg ggcgtgtaca tcggccagtc caccaagatt tacgaccgcg
     1081 aaaccggcga agtcacctat ggccgtgtcc cggccggctc cgtggtagtc agcggcaacc
     1141 tgccgagcaa ggatggaagc tatagcctgt actgcgcagt gatcgtgaaa aaggttgatg
     1201 ccaagaccct gtccaaggtc ggcatcaacg aactgttgcg cggggtgtaa tgaagctgta
     1261 cggcatcccc aactgtggca cggtgaagaa ggcgcgcgcc tttcttgaag cgcacaggat
     1321 tgcctatgat ttccatgact tcaagaagag cggcgtgagg gaagagagtg tagatcgctg
     1381 gctgcaacag accggctggc agaagctgct gaaaaagacc ggtcctacct ggagccagtt
     1441 gccggacgcg gtcaaggcga gcatcaacga cgatgcttct gccttggcac tgatgctgga
     1501 caaacccaat gtcatcaagc ggccggtgtt ggagcaggac ggcagagtcc tggctacggg
     1561 ctttgtcgag gccgattacg aaaggttgtt caaatgagcg caactctcga actttccaag
     1621 caactcatcg cccgccgctc cctcacgccg gacgatgccg gctgccagga aatcctgatc
     1681 gagcgcctgg aaaagctcgg cttcacggtc gagaagatgc gcttcggcaa cgtcgacaac
     1741 ttctgggcac gacgcggcac agccaagcct ctggtggtct ttgccggcca taccgatgta
     1801 gtgcctaccg gcccggtgga gcagtggctg agcgagcctt tcacacccac catccgcgcc
     1861 ggtatgctgt acggtcgcgg cgcggcggac atgaaaacct ccatcgccgc cttcatcatt
     1921 gcgctggagg agtttctggc gcggcatccc ggccacaacg gctccatcgg tctgctgatt
     1981 acctcggacg aggaaggcgt ggcggtggac ggcaccgtca gggtggtgga agcgctcaag
     2041 gcacgcggag agttgctgga ctactgcatc gtcggcgaac cgacctccaa caagcaggtt
     2101 ggcgacatga tcaagaacgg ccgccgcggc tcgctgtccg gcaaactggt cgtcaagggt
     2161 atccagggcc atatcgccta tccacatctg gtcaagaacc ccatccacaa ggccgcgccc
     2221 gcaatcgccg aactggcaca aacggtatgg gacgaaggca acgaatactt cccgccgact
     2281 tccttccagg tctccaacat ccgcggcggc acaggcgcta ccaatgtagt gcccggccac
     2341 gtggaaatcc tgttcaactt ccgctactgc accgccagca ccgagcaaaa cctgcgtgag
     2401 cgcgtgctgg ccatcctcga ccgtcacggg ctggaatatg acctggaatg ggaatccggc
     2461 aagccctacc tgacgccgaa aggagatttg gtcgccgtga tcagcgccgc catcgaacag
     2521 gcttacggca tcaccccgga actctccacc agcggcggca cctcggacgg ccgcttcatc
     2581 gccgacatct gcccgcaggt gatcgaattc ggcccactca acgccaccat ccacaagctc
     2641 aacgagtgcg tcggcgtggc cgacatcgac ccgctcaaga acacttaccg catcacgctg
     2701 gaaaaactgc tgaaatgagc tttcaccctc gcgctgcgga acagctgttc accctccgcg
     2761 actggctgcg ctttgccgtc agccgcttcg aggaggccgg catttttttc ggccatggca
     2821 cgcacaacag ctacgatgaa gccgtttggc tgattttgtc agcgttgcac ctgccgcatg
     2881 acacgttgga aaatttcctc gatgcggtag tgaccgagga cgagcgcaga gtacttgccc
     2941 acctgatcga acggcgcatc accgaacgca tcccgaccgc ctatctggta cgcgaagcct
     3001 ggctgggcga cttcaagttc tacgtagacg aacgcgtcat cgtcccgcgc tccttcatcg
     3061 cagagttgtt acgcgagcaa ctggcgccat gggtagaaaa cccggaagaa gtggtgagcg
     3121 tggccgacat ctgcaccggt agcggctgcc tcgccattct ggctgcccat gccttcccca
     3181 acgccaatgt cgacgccatc gacatttccg acgacgcgct ggccgtcgcg cgcaggaata
     3241 tcagcgacta cgggctagac gagcatgtac atccgatcaa atccgacatg ctgtcgtcat
     3301 tgatgggaaa gcactacgac atcatcatca gcaaccctcc ctacgtggac gccccctcga
     3361 tggagacact cccgcaggag taccgtcagg aacccggtct ggccctggcc agcggggatg
     3421 atggcctcga ccatacccac accctgctgc acaacgcggt caatcatctc aacccgggcg
     3481 gttatctgat cgtcgaaatc ggccacaatc gcgacgcgct ggaagccgcc taccctgatt
     3541 tgcccttcac ctggctggaa gtcagcggcg gcgatcagtt cgtgttcatg ctgacacgag
     3601 agcaactgat ctagcctgct gcatccagtc tgattcccca cgattgatct cgagcccatt
     3661 cgggctttga cattgattag ctggcttact ataatccgcc ttaccatgtt gcagctgaaa
     3721 aatcttcctg atgcgaaaat cctggaaaaa ttcgtcgcca gatacccgga cgcggaggtg
     3781 gaatccgtcc tgcctttcct gaacctgctc cgggctggtg cggatctttc ggacgcgctg
     3841 gacgcgctgc tcgcccagca tggcctgttg cagggccgct ggtgggtgct gattctgctg
     3901 atgcgggaag atgatctgat ttcgaccccc tccgatctcg cggaaaaggc cggggtctcc
     3961 cgtgccacca tgacgggcct gatcgatggc ctggaacggg atgggctggt acagcgattg
     4021 tccgatgaca gcgacaggag aaagctgacc atcaggctca ccgccctggg acaggccaaa
     4081 ctggatgacg tcatgcccag ctattaccag aaggtcaaac agctgatgcg cgtattaacc
     4141 cagtcgcagc gtgaagatct ggttgcacat ctcaaactgc tgaccgccaa tatcgaagcc
     4201 ctcaggtagc atttttttaa ccgattaatt agccagctaa ttacatgaac aaaggaaccc
     4261 tcatgaccac atccccacat accaccattg cgtccctgaa ctcgacctgg aaccagacat
     4321 tcaatcgcgg cgacgccgcg gcattagcca gactgtacgc agaaaatgcg accctctctc
     4381 cgggcaacgg ccagacgctg cttggacgcg ccgagatcga aaaactgttt cagagctttg
     4441 tcgatggcgg cgtacatcag cacacgatcg acatcgtgtc gaccggcggg gatgagcagg
     4501 tcatgttcca ggtagccaaa tggaacgcac atggcgcggc tcaggacggc aatacgccgt
     4561 cattcggcgg catcctgatg agtgcattcg agaaaggcgc cgacggtcaa tggctggtgc
     4621 gttcgcatgt ctggaacatg gcgggttgat gcctgaattg aaaaggagaa cagcatgcca
     4681 ttcgtgacca tctcgatcct gaaaggcaaa tcagcggagt acatccaggc ggttgcagac
     4741 ggcatcaacg ccgcagtgat cgaaaccatg gggtttcctg acgacgaccg ttaccaggtg
     4801 attcaccaac tggcgccgga atgcctgcaa ctgcaaacgc gcgaaggcga ccgtgtcatg
     4861 atgcatctgg tcatgcgcgc cggacgatcg aacaaatcca agcaggcgtt ttacaagaaa
     4921 gtcgtggaaa atctcgccaa caacccgggc ataccgccgg ccaacgtgtt gatcaccatc
     4981 accgaaaacc acgatatcga ctggtcgttc agggacggcg tcgcgcagtt tgtcgtctag
     5041 caccccagcc tgcaaacgca ggctggggaa tggccaactt cgattacccg cctcgaccgc
     5101 ggttgcgatc gctgcgcttt tgctgggctg gtggattcat ggcatcgcca cgcacgcgcc
     5161 gttttgaagc tggcgcgctg cgcgatggcg ccctggagtt cttggactga ggtaactcgc
     5221 tccgcttcga gggcttgcgt tccttcggcg taaacgtctt gcccaggctt tcccgttcct
     5281 tcggggtgcg ctgcggtgaa cgcggcacgg ccagcccggc ccattccagc aagcccgcca
     5341 catccttgga cggcaattcg agcatctggc cgcgcttgag gcgtgcaggc aaattgaccg
     5401 ggccaaagcg tacgcgcatc aatcggctca ccatcaggcc cagcgcctcg aataggcggc
     5461 gcacttcccg gttgcgacct tccttcagca tgacctcgta ccagtggttg gcgccctcgc
     5521 cgccccggtc ctggatcttg tcgaactggg ccatgccgtc ttccagttct acgccctgcg
     5581 tcaattgcag catctgggca tcggtcagct cgcccatcag gcgcaccgcg tattcgcgtt
     5641 cgacctcata gcggggatgc atgaagtggt tggccagttc acccgaggta gtgaaaatca
     5701 gcaaacctga ggtattgatg tccagacgtc cgatggccac ccagcgaccg ctgcgcacat
     5761 gcggcagctt gtcgaatacg gtggcgcgtt tttccggatc gtcctggctg acgatttcgc
     5821 cttccggctt gtggtagagc agcacgcgcg gcaattcagc ctcgaacggc aaatgcagcg
     5881 ggcggctatc cagcctgacc acatcggacg gcaccacgcc cgcgccgacc tgtgccgtct
     5941 ttccgttgat ggtgatgcgg ccgctggcga tcagttcttc catgtcgcgg cgcgaaccca
     6001 ggccggccag cgccagcagc ttgtgcaggc gccgagatgg ctggtccggg ttggctgcag
     6061 gctgcggacg tggcgtgcgg gtttgtttga atgggcgtgt cataagtcca gcggtatctc
     6121 gtgttgggaa aaatgttcca gcggcggcag ttctgccagg gaacgcaggt taagatcgtc
     6181 gaggaaagtc ctggtggtag caaacaagga aggccgtccc ggcacctcgc gatggccgac
     6241 cacgtcgatc cagtcgcgct cctgcagcgt cttgatgata ttggggttga ccgacacgcc
     6301 gcggatttcc tcgatatcgc cgcgtgtcac cggttggcgg taggcaatga tcgccagtgt
     6361 ctccagtacg gcacgcgagt agcgctgcgg cctgtccgga ttcagtcgcg ccaggtgggg
     6421 ccagtattcg gcgcgcgcct ggaagcgcca gccgctggcc acctgcgcca gctccatggt
     6481 acggctgttc cagtcagttt tcagctcgtc cagcagcatg cgcagggtgg cgcgctccat
     6541 ccgctcctca aacaggctgg ccagttgatc cagcgacaaa ggcgcgcggc tggtcagcag
     6601 cgcggcttcc agcacgtttt tcatctgctg gatatcagag gacatcgttg gcctgcacat
     6661 aaatcgggga aaacgccgcc gcatcctcat gcatctgggt cagccgcacc aggccttccc
     6721 gcgccagttc cagaatggcg aggaaacaca ccaccagctt gggcacgcct tcgccgacct
     6781 cgaacaacgc ggtaaattcc agcagcggct tagcgttcag cctgcgcaga atcaggctca
     6841 tgtgctcgcg caccgacagc tcctcacggc tcaccttgtg gtgcacgtag tggctggcac
     6901 gcctcagcac ttcctgccag gccatcagca ggtcttccat agacacaccg ggcaactgct
     6961 gttgcgggat gtgctcgaaa taggcttgcg ccaccagcag ttcgcgccct acctgcggca
     7021 gttcgtccag cttgtgcgcc gcaagcttca tggattcgta ttccagcagg cgccgcacca
     7081 actcggcgcg cgggtcatct tcctgctcga tttcggccgg tttcggcagc aacatgcgcg
     7141 acttgatctc gatcagcatg gccgacatca gcaggtagtc ggcagccagt tccagattcc
     7201 tctctcgcat ggtttccaca tacgccatgt actggcgcgt cagatcagcc atgggaatgt
     7261 cgaggatatc gaggttatgc ttgcggatca gatacagcag caggtcaagc ggcccctcga
     7321 aggcttccag ataaacctcc agcgcatccg gcggaatgta gagatcctgc ggcaactctg
     7381 caagaagttc tccatggatg cgggcgtggg gtgaggatat aagcatttgc gtctaattcc
     7441 cgaatggaat ttccaaatcc ttgcatatct tgcgcgccag catttcgtta atctcacgat
     7501 gccgcggcac cgcagaaacc ttgcccttag cgcgattaac gaaaatactg tggctcccgc
     7561 cctcacgcag caaatcgcaa gcattcgcct gcagatggcg gattaaatcc aggcgcttca
     7621 tgcaaccgca agattcaggg gttcgcgtat cacctcatgg ccggccaacg actcttcggc
     7681 taacacgcga ttcgcttcca gcaccaactc caccgcctcg gcaagattcg ccctggcttc
     7741 ttccagtgtg tcaccttgtg tattcgctcc tggcagttcc tcgacaaacg cgatatagcc
     7801 ttctggtact ttttcaaata ctgcggtcaa ctggtagttc atttagagca ctcctaattg
     7861 attgctttca taatctatga tttttgaaag aattagccat tttaatcgtc aaactcaaat
     7921 gcctcgtcag cattaatcat tccgccatta cacaccagag atgcggcacg agccacgata
     7981 actgcacgct tttttgtgaa aacttcaaaa tcagaacgca gttttgcttt ttgctcttcc
     8041 gtgttcagat ccgagtatcc aactgcaaaa tcatcgaagg gaattaggtg ggaacgtaag
     8101 cggcgtttaa tctcatcctc accaagagcg ctattgtctg tacgttcctt gaggtagatt
     8161 aaaggctctt tgttcgagat cgtgcgattc gttctccaag tgaccaaagc acaattaact
     8221 gctcggaata tctgctcggc gggtactcct gcatcagaca gaagcgaatc agggaacaaa
     8281 tgatgatact cgcgtggatg agtctttgac gtaattgagg caacagttgc gggtttcgca
     8341 tctgcaaaat cgatagcacc gcatttgagt tgaagcgcca acactccgcg ccccaagatt
     8401 gtttttgact ttggccagcc agcctgaaga atcagatccg ccgttggaag cggataagac
     8461 tcctgattca gcacaggtac atctttctct gtaccttggc caagcaaaac tttcttcaaa
     8521 gcccgaaaat cctgtaatgc accgctactg gaagcttgct cataacgcgc agttaaaaaa
     8581 gcacgccaca agtattttct taacaacgcc ctagcgttgc cgagctgatc aggttgcgtc
     8641 ggtagcattt cccaaagtgc ggcaataacc ggtagtgggg tataactagg aagccgctgt
     8701 gcatcgaata tgctttcttc ctcaagaaac tcaacaagcc cctttacgct ctgaataatc
     8761 attggccagt ctccaaccat cccgggatag tcgatacctt tataaccagc ctgactaggg
     8821 acacggtctt gccgcaatgc ttcaagatca agaacgaggc ttggcaaatc ggcatattca
     8881 gccgctctag ggcaagcgtc ggcaagctca tccacgtgct catgcaatga cttgcctgtc
     8941 tcaccttcga ccagagcaac aataacgtca taagtcgtca gcttgacgga gctcgtgttc
     9001 atcttgacga atacgtctag tgcgacctct ttattggtcg ttactggcag tgcaaggtaa
     9061 gggagattaa attcacgcac tttggtacga aggccattga ttgtgttctt gaccttgttg
     9121 aaagactgga gtgagtcctt ctggtcatcg ggtagcgccg cagtcaccca gctatcgaca
     9181 tcggtatcat tcgcgcctgg taccaacagc ctgaggggga ttcttcctcg gttccaacat
     9241 tcacgcggag aatcaaccca aattggaaaa cgaaggccgt tcttaaccca tcttgcttgc
     9301 ccctcgacga ccggttgttt ttctgctcca gcatctgaag ggtcgtcttc aaagtaaatg
     9361 agatacgtcc ggtcagggta cttgtcatgc aggctacgcc acagcgcagt aagtcgctgt
     9421 tgaccatcga gcaactgttc ggtaacgcga tcacccaact cgggagcatc ggtcatggtg
     9481 cgacttttga atttctcttt atcgccaact tccaggatga gagcagcccc tgacggtaaa
     9541 ccccgtagca ctgtggttag caagtcagaa acattgtcat gcccccaagc cacatatctc
     9601 tggaatctag gaagggtaat ttgtcgagtg cggatacgat tgaaccattc agggagaaat
     9661 cggttgtgtg cttccatttt ctatttatcc gtttttgttg ttggtagatc aatttttacg
     9721 agtagctcaa ccccatcgcc tcgcgcacgt cgcgcatggt ttcgcgcgcc agcttgcgcg
     9781 ctttttcaca accgtcagcg acgatgttct tcaccagggt cggatcttcg gcgtattgcg
     9841 cggcgcgttc ctggatcggc ttgatctcgg ccagcacggc gtcgatcact ggctgcttgc
     9901 actcgatgca gccgatggaa gcgcctttgc agccttgctg cacccaatcc ttacgctgct
     9961 cgtcggaata cacttcgtgc agctgccaca ccgggcactt ggccggatct ccggcatcgg
    10021 tacggcggac gcgcgccgga tcggtaggca tggtgcggat tttcttggaa atcacgtccg
    10081 ggtcttcgcg cagcgaaatg gtgttgttgt aggacttgga cattttctgc ccatccagcc
    10141 ccggcatttt tgaagccggc gtcagcaccg cttccggctc gaccagaatc atcttgccgc
    10201 cgccttccag gaaacccagc aggcgttctc ggtcacccag gctcaggcct tgctgctcgt
    10261 ggatcaaggc acgggcagct tccagcgcct cgtcgtcgcc gctttcctgg tagcgtgtac
    10321 gcagttcctc atatagcgcc cccttcttgt tgcccagctt cttgatggcc gcctcggcct
    10381 tttcctcgaa gcccggctcg cggccgtaaa tgtgattgta gcggcgcgcg atctcgcggg
    10441 tgaatttgat gtgcggcacc tgatcctcgc ccaccggcac ctgggtggcg cggtaaatca
    10501 ggatatccgc gctttgcagc agcggatagc cgagaaaacc gtaggtggac agatccttgc
    10561 tcgacagctt ttcctgctga tccttgtagg tcggcacacg ctccagccag cccagcggcg
    10621 taatcatgga cagcaaggta tgcagctcgg cgtgttccgg cacgcgcgac tggatgaata
    10681 ccgtggcact ggccggatca acgccggcag cgagccagtc aatcaccatt tcccacacgc
    10741 tttcctcgat gatctgcggc gtgtcgtaat gagtggtgag cgcatgccag tcggcgacga
    10801 agaagaagca ctcgtgctca tgctgcaact tgagccagtt tttcagcacg ccgtggtaat
    10861 gccccaagtg caggctgccg gtgggcctca tgcccgaaag aacgcgttca accgccatga
    10921 aacggaatcc cttagaaatt gaataacacg gataacagac cgatcaccgc ataaatgaac
    10981 ggccccagaa ttttaccgag aataccggta aacagcagca caatcaggat aatgaaaccg
    11041 tagcgctcga tctgtgaaaa cttccacgcg tagggctgcg gcagcagact gaccgcgatg
    11101 cgcccgccgt ccaggggcgg tagcggaaac aggttgagca ccatcagcac cacgttgatc
    11161 atgatgccgg cctgcgccat cagaaccaat ggcgttgcaa tggcctcgga aaacgccggg
    11221 gccagcttga gcgccagcgc ccacaatatc gccatgacca ggttggaggc aggtccggcc
    11281 gcggccaccc acagcatgtc acgcttggga ttgcgcaggc ggccgaaatc gactggcacc
    11341 ggcttggccc agccaaaaag aatgccgccc gccatcatgg tcagcagcgg caccagcacg
    11401 gtaccgatgg gatcgatgtg gcgcagagga ttgaagctga tgcgccccgc cgccgccgcc
    11461 gtcatatcgc cgaaatagcg tgccacgtaa ccatgcgccg cttcgtgcac agtgatagca
    11521 aagatcaccg gcaaagcata caccgtaatt ttttgtatca gggagagttc catatcattc
    11581 cagtccaaac ggggcgggat cgcccctacc gcgccgcacc aatactggct cggcaccggt
    11641 caggtcaatt actgtggtca tttcgtgcga gcaggcgccg gcgtcaatca ccaggtctac
    11701 ccggctttcc aactcctcgc ggatttccca cgcctcgcgc ggagcatccc agccgggcag
    11761 cgtcagggtc atgctgagca gcggctcgtt cagatcttcc agcaacgcca gcgtgacggc
    11821 atgatccggc acgcgcaggc cgatggtgct gcgcctggga tgctgcaggc ggcgcggcac
    11881 ttcgcggctg gcttgcagga tgaaagtgta gctgccgggc gtattggcct tgagcaggcg
    11941 gaaccggctg ttgtccacct tggcataagt ggcaatctcg gccaggttgc ggcacaccag
    12001 ggtgaaatgg tggtgctcgt ctacgccgcg gatggcgcgg atgcgctcct gcgcctcctt
    12061 gtcaccgaga tgacaaccca gtgcgtagca ggaatcggtg ggataggcca ccacgccacc
    12121 gttgcgtatg atctcggccg cctgatgaac caggcgcggc tgcggattgt ccgggtggat
    12181 ggaaaaatac tgtgccatga tgtcaggctg ccagtcgcat ttccggccag tcgtgccaca
    12241 ccggcgtaca gttggcgggc aagtcaggca gccggcccat atcggtgtag gtctggccgg
    12301 ggccatggta atccgagccc agcgacgcca gcaggccgaa ctggtggctc aagcgagcga
    12361 agtgatggta ctgctctggc gtatgactgc cggtcaccac ttccacgccg gttccgccca
    12421 gttcacggaa ttcggccagc agcttgagca tgttgacgcg gcccaggtca tagcgcccgg
    12481 gatgcgccag caccgcgatg ccgccgctgc cggtgatcca gcccaccgca tcttccagtg
    12541 ccgcccagcg atgctcctgg tagccgggct tgcccttgac caggtatttc ttgaacacgg
    12601 cagagacatc cttggcatag ccctgctgta ccaggaaacg cgcgaagtgg gtacggctga
    12661 tgatgccaca ggtggcgtaa gcgtgggcgc catccaggct gccgtggata ccggcctgct
    12721 ccaggctggc ggccatgccc tcggcgcgct ggtagcgccc ctgccggatc gtcgccaggc
    12781 cttgtagcag cggaatatgg gtgggagaaa ttttcaggcc gaccacgtgc acagtacgcc
    12841 cgttccaggt cacggaaatt tccacaccgt taaccagcgc gataccatgc cgtacagcgg
    12901 cttcggcagc ctcttccagt ccggccacat cgtcatggtc ggtcagcgcc agcacgcgca
    12961 cgccgcgcgc ggcagcgtgt tcgacgagtt cagaaggcgt gagtgtcccg tcggagacga
    13021 cggagtggct gtgcaggtcg attggagatg ccatggaact tatcttgtga ggtaggccaa
    13081 atcatagcaa aatagccgcc ttaccgaaac ttttgcggca ttttagcgat tccaccccac
    13141 atgaaattcc tcttcgatct attcccgatc atcctgttct tcgtcgtgtt caagagcgta
    13201 ggcatcttcg ccgccaccgc caccgccatt gccgccacgc tggcgcagat cggctgggtc
    13261 aagtaccgcc atggcaaggt cgatggcatg ctgatcgcca gcggggtgat tatcgtcgta
    13321 ttcggcggcg cgaccctgct gctgcacgac gagaccttca tcaagtggaa gcctactgtg
    13381 ctgtactggc tgtttgccgt agtacttttt tccgctgaac tgttctggaa caagaacctg
    13441 atccgctcgg tgatggaaca gcagatcagc ctgccgcaag cggtgtggcg caccctcaac
    13501 cacgcctggg cagctttctt cgccgtgctc ggcctgctga acctgtacat cgcgttcaac
    13561 ttcgccaccg atacctgggt gaacttcaag ctgttcggca ccacggcgct gatgctggtg
    13621 ttcgtagtga tgcaaagcct gctgctggga aaatacgtaa aggatcagga ataatgcttt
    13681 atgccatcat tgccgaagat gcgcccaaca gtctggaaaa acgcttggcc aatcgcccac
    13741 cacatctgga gcgcctccag cagttgcagc aggaagggcg cctactgctg gccggcccct
    13801 tccctgccat cgacagcgtc gaccccggcc cggctggctt cagcggcagc ctgatcgtcg
    13861 ccgaattttc ctcgctggaa gacgcgcaag cctgggccga agccgacccc ttcgtcgccg
    13921 gcggcgtgta tgccaaggtc acggtgaagc cgttccgcaa gacactgccg gcctgatggc
    13981 gctgccggac gaaatacggg agcgactggc catcctcgag ccactgcaac tcgagatcga
    14041 ggatgacagc gcaaggcacg caggccatgc aggcaaccag ggcggcgggc atttcaatat
    14101 caccatcgtc agctcgcatt ttcttcagaa atcgcagata atgcggcatc gcattgttta
    14161 tcaagcactt aacgacctca tcccgtcccg catccatgcc ttgagcatac gcgccctgac
    14221 tccggacgaa atttgacttt cactgcagcc cttacaagga aaaccgtacc atgaccaaac
    14281 gcatcctcgt cgcccttgcc ttaaccggcc tgcttgcccc agccgtgcat gccgccgatc
    14341 cagcaaccgt caatggcaag ccgatcaagc aatccctggt ggacttcgtc ctgaaggacg
    14401 ccgcggcgca agggcagaaa atcgatgacc aatccaagtc gctggtactc aaccgcttgg
    14461 tcacccagga ggtggtgatc caggaggcgc agaaagccgg gctggacaag caggcggatt
    14521 atctggccag acaggaactg gccaaccgcg aactgctgat caatacctat ctgcaggact
    14581 tcatcaaaaa gaaccccatc agcgaagccg acatcaaggc cgaatacgac aagttcaaac
    14641 agcaattggg cgacaaggaa tacaacgccc gccacatcct ggtcaagacc gagcaggagg
    14701 ccaaggacat tatcgcccag ttagccaagg gtgccgattt tgccaaactg gccagggaaa
    14761 aatcacagga tcctggctcc aaggacaagg gcggcgaatt ggactggttt tctccggcac
    14821 gcatggtcaa gccttttagt gacgcagtgg ttggcatgaa aaaaggcgag gacaccaaga
    14881 ctccagtaca aaaccagtat ggatggcatg tcatcaagct ggtcgatgtt cgcaaaacgc
    14941 aggatcccgc ttacg
//
LOCUS       DAOMFA010000021        14430 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_359_length_14430_cov_5.944696, whole genome shotgun sequence.
ACCESSION   DAOMFA010000021 DAOMFA010000000
VERSION     DAOMFA010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 14430)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14430)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14430
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_359_length_14430_cov_5.944696"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..521
                     /locus_tag="PKW44_02080"
     CDS             <1..521
                     /locus_tag="PKW44_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018076356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HQR50408.1"
                     /translation="CIFNNVAVGVAHAMAKHGIERAAIIDFDVHHGDGTEDIFQANPQ
                     VMLCSTFQHPFYPYKGADSRSANIVNLPLPAGTDGKKYREAVEREFAPALEQFKPQMV
                     FVSAGFDAHTQDPLADFRLVRDDYVWITEFILQIAGKHAEGRVVSTLEGGYDLTSLAD
                     SATAHVRSLAGL"
     gene            538..1992
                     /locus_tag="PKW44_02085"
     CDS             538..1992
                     /locus_tag="PKW44_02085"
                     /inference="COORDINATES: protein motif:HMM:NF025055.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pili methyl-accepting chemotaxis
                     transducer N-terminal domain-containing protein"
                     /protein_id="HQR50409.1"
                     /translation="MAALKLREKITGLLVFYFFVALIAIGSTLFVSWQLEGGAAAIND
                     AGRERMRSYRIAFLLSQQVRHPSFELRRDIEQEIMLFEQTLTELERGNPQRPLSLPKD
                     AKVRQQMTQLRKDWQTEIKPRLLHILETTQASEQGRLLDEYRQPLEHFVAGINELVAM
                     IEHSNAHATTVLRIFQIALVSLALIGTVLLVYLFALMVVRPVARLQEGIQRMGKAEFE
                     VRLPVTSSDELGELAEGFNRMAGELQDIYATLEQRVEEKTRSVEAKNRELAALDKEMA
                     VSEERNLLAQELHDSIAQSLAFLNIQVQLLHEDLQKNRTMEALQGLEQIREGVQESYD
                     DVRELLVHFRTRIGSTDLETALRSALEKFEAQTGIHTTLILDGPSPQLLPEQVLQVMH
                     IVQETLSNVRKHARANRVDVELSGGKRCSVIIRDNGAGFDAARDAGDTHVGLRIMRER
                     AHRIGAELTLESTPGQGTRICLGLQENPQESTSE"
     gene            1989..2648
                     /locus_tag="PKW44_02090"
     CDS             1989..2648
                     /locus_tag="PKW44_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019898586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HQR50410.1"
                     /translation="MKPIRVLIVDDHTLFRSGIKLLLQRQEDFEVVGEAGDALEGVKL
                     AKRLKPDVVLQDLHMPGTGGLQAITLLREEAPQSQIIMLTVSEDAEDLLEALRAGARG
                     YLLKNIETEFLMDSIRRAAAGESVMSPQMAGKLADAMRAPPKGAPAIAPDANRLTPRE
                     REIIVMLARGASNKEIARELDLAESTVKIHVQGILRKLNLASRVQAAVFAVEHGLVAE
                     K"
     gene            2860..4047
                     /locus_tag="PKW44_02095"
     CDS             2860..4047
                     /locus_tag="PKW44_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HQR50411.1"
                     /translation="MATQQYKAWSVVGMSTLSFTVCFMVWMMFAVIGIPIKATLGLNE
                     TQFGILVATPVLTGSLIRLPLGMWTDKFGGRVVFFVLMLSTIIPIWFISRCTEFWQFL
                     VTGLFVGVAGGSFTVGIAYCARWFPKNRQGLAMGIFGAGNTGAAVTKLVAPSIVVAYG
                     WAMVPQVYAAIMTVTALLFWFFTYSDPAHKVDKAVTMREQLATLKDPRVWKYSQYYSI
                     VFGGYVALALWMTKYYVSEYGFDLKTAALLAAAFSIPGGVLRAIGGHFSDKFGAHTVT
                     WWVLWVALVCLFFLSYPQTDVSIMTVKGPQTFHIGLSAAWFTIIMFTMGIAFAIGKAS
                     VFKYISDDYPHNIGVVSGVVGLAGGMGGFLMPVMFGALVDVAVVPSSSFMLMFGIVWV
                     SLI"
     gene            4132..5742
                     /locus_tag="PKW44_02100"
     CDS             4132..5742
                     /locus_tag="PKW44_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019898582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiporter"
                     /protein_id="HQR50412.1"
                     /translation="MSTEIKEWNIEDEKFWESTGKKVAYRNLWISVPALLCGFAVWLM
                     WGIITVQMSNLGFPFSKDELFTLTAISGLAGATMRIPSSFFIRLAGGRNTIFLTTAML
                     LSPAIGTGIALQHPEWPLWVFQLMALWSGVGGGNFASSMSNISTFFPKRLQGTALGIN
                     AGLGNFGVTTMQILIPLLMTVSVFGAMGGDSMLLVKDSGWIFGKIPAGTPTFIQNAGF
                     AWMLSLVPLAILCWFGMNNLKTISPDIGGTIVAFIKITWLYTLAFVPAIAGLYLYLPA
                     PSGLGLLNMWVAMPLIIMATLLVMKLAAFGTMKENIAKQFAIFGNKHTWSMTALYLVT
                     FGSFIGFSMALPLSITVIFGDTHTLDAATGMWTHAKNPNAPSALTYAWIGPFVGALIR
                     PVGGWISDKLGGSIVTQVISAVMVVASIYAGHIMMQAYHSATPEIYFSQFLITFVVVF
                     AAAGVGNGSTFRTVGVIFDRVQAGPVLGWTSAVAAYGSFIAPEVIKGQIKAGTPEMAM
                     YGFAVFYALCLILNWWFYLRSGAEIKNP"
     gene            5779..9534
                     /locus_tag="PKW44_02105"
     CDS             5779..9534
                     /locus_tag="PKW44_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019898581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HQR50413.1"
                     /translation="MSHFLDRLKFFDKVKATFSNNHGIVTNEDRQWEDAYRHRWQHDK
                     VVRSTHGVNCTGGCSWKIFVKNGLVSFEMQQTDYPRTRDDLPDHEPRGCQRGASFSWY
                     LYSPHRVKHPLIRGRLLDLYRAERKTGKDPVEAWAAIQADEKKRKSYTAVRGLGGFVR
                     SSWDEVNEIIAAANVHTIKKWGPDRIFGFSPIPAMSMISYAAGSRYLSLIGGACGSFY
                     DWYCDLPAASPQTWGEQTDVPESADWYNSTYIIITGANLPMTRTPDAHFAAEVRYKGA
                     KIVAMAPDYAEFCKFSDLWMPIRQGTDTAAFLAMGHVALKEFYIENQDPYFQEYARKY
                     TDLPMQVMLRKQGDAYVSDRFLRASDFNGALGESNNPDWKTIVYDEKSQSFIAPNGSI
                     GFRWGEDGKWNLLEKGGASQQDTTAQLSCIDTRDDVVSVGFPHFNQGEDALLFRNVPV
                     RKVKLANGEEALVATVFDLQIAQYGIDRGLGGGNVASNYADANVAYTPAWAEKVTGVK
                     AADIERTGREFAYNASRTKGRSMVIMGAAINHWYHNDLAYRSIMNLLHICGCVGQTGG
                     GWAHYVGQEKLRPQAGWAPIAFATDWHRPPRHTNSTSYWYFHTDQWRYETLKADSLLS
                     PAGKGKNKGNSLADYNVKATRMGWLPSMPNFNRNPIELAEEALASGAADEAAVAKYVA
                     GQMKSGALDVAYADPDNPVNWPRNLFVWRANLIGTSAKGHEYFLKHLLGAQNGVLQES
                     GAGRDNKEVKWHEEAPIAKLDLMVDINFRLNSTGAYSDIVLPTATWYEKNDLNTTDMH
                     PFIHPLSEAVSPGWESKSDWQIFKTIAKTFSTLAEKHLGTKKEVVTLPMLHDSAAEMA
                     QPFGEVKNWKEGDCDPIPGKTMAIIKLVERTYGDTYRKYIALGPLMQKLGNNIKGIDW
                     NTDQEYEELKKHNATVKEAGVSFGMPSLEEDIYVCDSVMRMAPETNGEVAHKSWNSLS
                     IKTGIDHHHLYAGRHEDKITFKDIQAQPRKIITAPTWSGIESETVSYTAGYTNIHEHI
                     PFRTLTGRAHFYQDHEWMLDFGEGFCTYKGMVDLGELNSLPANVINKPHLEVNWITPH
                     SKWGIHSSYQDTLRMLTLFRGGPYVWISETDAKSIGLEDNDWVEAVNHNGATVARAVV
                     SQRVPQGMAMMYHAQEKNVNVPGSPSTGKRGGILNAVTRVIIKPTNMIGGYAQLAYGF
                     NYYGTVGCQRDTMVAIHKIADKDVDWLERPLTPEREAQLNPVGIGPR"
     gene            9554..11101
                     /gene="narH"
                     /locus_tag="PKW44_02110"
     CDS             9554..11101
                     /gene="narH"
                     /locus_tag="PKW44_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HQR50414.1"
                     /translation="MKVRAQFAFVFNLDKCIGCHTCSVTCKNVWTNRKGVEYAWFNNV
                     ESKPGIGYPKQWENQEVWNGGWELKNGKLELKSGGRVNKLLNIFANPDLPEIDDYYEP
                     FTYNYAHLQNAPLSEATPTARPISQITGESMEKITWGPNWEDDLAGEFHKRSKDKNLD
                     NIQKEMYSQFENTFHMYLPRICNHCLNAACVAACPSGSIYKREEDGIVLVDQDKCRGW
                     RMCVSSCPYKKVFYNWESGKAEKCIGCYPRVESGMPTVCSESCVGRIRYNGIMLYDAD
                     RIEELASIENEQDLYEAQCRIFLDPNNPEVIAAARAEGINDDWLEAARKSPIWKMAMD
                     WKIAFPMHPEFRTLPMVWYVPPLSPVQSQIDQGNLPVGPDGAIPVAGTMRLPVQYLAN
                     LLTAGKTAPIESALNRLIAMRSYQRSVHVEGKADTRAIDAAGITEDQAKEMYRYLAIA
                     NYEDRFVIPTGHTEETLNDAYGFQGQNGFTFGNDSSSGVSKITLFPRRRKETVEPKEL
                     APSGDNR"
     gene            11111..11629
                     /gene="narJ"
                     /locus_tag="PKW44_02115"
     CDS             11111..11629
                     /gene="narJ"
                     /locus_tag="PKW44_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019898579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HQR50415.1"
                     /translation="MRLYKMLSVLLEYPDQELLDNLPELRSEIDACIDMDDAERSAMR
                     HFLIHLEQTSLTDLQADYVKTFDLTAENSLHLTHHLFGDDKNRGPALIDLGEMYKDYG
                     VEVVTNELPDYLPLILEFAAYLDDSEASVFLADANKVLGVLADNLKQAGSPYAGLLFI
                     VEGRASLTRLAA"
     gene            11643..12332
                     /gene="narI"
                     /locus_tag="PKW44_02120"
     CDS             11643..12332
                     /gene="narI"
                     /locus_tag="PKW44_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008061875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HQR50416.1"
                     /translation="MNLHNFLYGVYPYIALSAFLLGSWLRFDREQYTWKSDSSQLLSK
                     AGMRLGSNLFHIGILAIFGGHLVGLLTPHSVFRSLGVSDMNHQMIAIWAGSIFGLLCL
                     AGGAILWLRRMTNARVSAASRGSDKFILTWLLLTLMLGLSTIPVSIGHANEGNPGVMI
                     ALAEWVQSIIYLHPNPALLDGVDTIFKVHLFFGMTVFLVFPFTRMVHVWSAPFGYLGR
                     PYQVVRSKRYH"
     gene            12341..13033
                     /locus_tag="PKW44_02125"
     CDS             12341..13033
                     /locus_tag="PKW44_02125"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019898576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HQR50417.1"
                     /translation="MRDVSKFLSGFRRFQHHYLGARRAYFDRLIKEGQHPTALVVACC
                     DSRCDPALLTDCDPGDMFVVRNVANLVPPYTQAGSFAATSSAIAFAVHQLEVEHVIVM
                     GHAQCGGIRTLIEHKPVACDEDQLIAKWLGIAEDARQKAMQALPGRTSETQARACEQA
                     SILVSLENLMSYPWIKSRVEAGKLLLLGWYFDMVNGELLQYEADPGEFRALVSHEGIG
                     KSTEHLWWRAPG"
     gene            13030..14286
                     /locus_tag="PKW44_02130"
     CDS             13030..14286
                     /locus_tag="PKW44_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HQR50418.1"
                     /translation="MKPTTSISLAQIREPARPRPSDSGGRWRHFTAMPHHVLFAIGAV
                     QAVLVMLWWLVDLGGRYGGWYAPIDWTLPSPWAHLYLMVYCLFPPYMFGFLMTTYPKW
                     MAGVPVARKHYLATAALLGTSALLGYVGLATEKSWLLLAMGLHLAGWATGIYALLRVY
                     AGAQRPDTLHALITSGVLLLGWGLLLTYISGVQTGNAGLVAIARSGGIWWFLLPVFFS
                     VSHRLIPFFSSVVLPDYQIIRPDWTLWLMSAGSLVHGTLELLGQEQWTCLVDLPMAVV
                     AAWLSYVWCFRKSFQTRILAMLHMAFAWLSVALALYGLQSLSLPLGRAPLHALVVGYF
                     ASMLVAMATRVTLGHSGRPLTADNLAWGIFLMVQAAALLRVLADLPVINAYAAHLYLC
                     SALLWLAAFISWLIKFAPIYWRPQST"
BASE COUNT         2990 a         4418 c         4165 g         2857 t
ORIGIN      
        1 tctgtatctt caacaatgtc gcggtaggcg tggcgcatgc catggcgaag cacggcatcg
       61 aacgtgcagc catcatcgat ttcgacgtac accacggcga cggcaccgag gatatttttc
      121 aggccaatcc tcaggtcatg ctgtgctcga ccttccagca cccgttctac ccctacaagg
      181 gggcggacag ccgtagcgcc aacatcgtca atctcccctt gcctgccggc accgacggca
      241 agaaataccg tgaagctgtc gaacgtgaat tcgcgccggc gctggagcaa ttcaagccgc
      301 agatggtatt cgtctccgcc ggattcgacg cccacacaca agatccgctg gccgacttca
      361 ggttggtacg tgacgactat gtgtggatca cggagttcat cctgcagatt gccggcaagc
      421 acgccgaggg gcgggtggtt tcgacgctgg aaggcggcta cgacctcacc tcgctagccg
      481 acagcgccac ggcacacgtc agatcgctgg cagggctata ggtcatctca cctcaccatg
      541 gccgcgctta agttacgcga aaaaatcacc ggcctgctgg tgttctattt ttttgtagcc
      601 ctgatcgcca tcggttcaac gctgttcgtc tcctggcagc tggaaggtgg cgcagcagcg
      661 atcaatgacg ccggacgtga acgcatgcgc tcctaccgca tcgcctttct gctttctcag
      721 caggtgcgcc atccctcgtt cgagttgcgg cgcgacatcg agcaggaaat catgttgttc
      781 gaacagactc tgaccgagct ggaacggggc aacccgcagc gcccgctttc cctgcccaag
      841 gatgccaagg tcaggcagca gatgacgcaa ttgcgcaagg actggcagac tgagatcaag
      901 ccgcgcctgc tgcacatcct ggaaacgacc caggcctcgg agcaaggacg cctgctggat
      961 gaatatcgcc agccgctgga acatttcgtc gccggcatca acgaactggt agcgatgata
     1021 gaacacagca acgcccacgc caccacggtg ctgcgcattt tccagattgc cctggtcagc
     1081 ctggccctga ttggcaccgt gttgctggtt tacctgtttg cgctgatggt ggtgcgtccg
     1141 gttgcccgtc tgcaggaagg gatccagcgc atgggcaagg ccgagttcga ggtacgtctg
     1201 ccggttacca gcagcgacga gttaggcgaa ttggccgaag gcttcaaccg catggcagga
     1261 gaattgcagg atatttacgc cacgctggaa cagcgcgtgg aagaaaaaac ccgcagcgtg
     1321 gaggccaaga accgcgaact ggcggcgctg gacaaggaaa tggccgtgtc tgaggaacgc
     1381 aacctgctgg cgcaggaact gcacgacagc atcgcccagt cgctcgcttt tctcaacatc
     1441 caggtacagt tgctgcacga agacctgcaa aagaaccgta ccatggaggc gctacaaggg
     1501 ctggagcaga tccgcgaagg cgtgcaggaa agctacgacg acgtgcgcga actgctggtg
     1561 catttccgca cccgcatcgg cagcaccgac ctggaaaccg ccctcaggag cgcgctggag
     1621 aagttcgagg cgcagaccgg catccatacc acgctcatcc tcgatggccc ttcaccacag
     1681 ctactaccag agcaggtgtt gcaggtcatg catatcgtgc aggaaacgct gtccaacgtg
     1741 cgcaagcacg cccgcgccaa ccgcgtcgac gtggaactat ccggcggcaa aaggtgcagc
     1801 gtgatcatcc gcgacaacgg cgctggcttc gatgccgcgc gtgacgccgg cgacacccat
     1861 gtcggcctgc gcatcatgcg cgaacgcgca caccgcatcg gcgcggaact gacgctggaa
     1921 tccactcccg gccaaggcac ccgcatttgc ttggggctgc aagaaaaccc acaggaaagc
     1981 acttctgaat gaaaccgatc cgcgtcctga ttgttgacga ccataccctg ttccgcagcg
     2041 gcatcaaact gctgctgcaa cgacaggaag acttcgaagt ggtgggcgag gcaggagacg
     2101 cgctggaagg cgtcaagctg gccaagcgac tcaagccgga cgtggtgctg caagacctgc
     2161 acatgcccgg caccggcggc ctgcaagcca ttaccctgtt acgcgaggaa gcgccacaaa
     2221 gccagatcat catgctcacc gtgtccgagg atgccgaaga cctgctggaa gcgctgcgcg
     2281 ccggcgcgcg cggctatctg ctgaagaaca tcgagaccga attcctgatg gactccatcc
     2341 gccgcgccgc tgcgggggaa tcagtcatgt cgccgcagat ggcaggcaag ctggcggatg
     2401 ccatgcgcgc cccgcccaag ggcgctcctg ccatcgcccc agacgccaac cgtctcaccc
     2461 cgcgcgaacg cgaaatcatc gtcatgctgg cacgcggcgc cagcaacaag gaaatcgccc
     2521 gcgaacttga cctggcggaa tccaccgtca aaattcacgt gcagggcatc ctgcgcaaac
     2581 tcaatctcgc cagccgcgtg caggcggcag tgttcgccgt ggagcatggc ctagtagctg
     2641 aaaaatagcg gggcctatgg cagatcggac agtagctata tctgaatcag tactaattat
     2701 gactaaagca tctcttgatc gatccactcg gaccaatcgt cctggtagtc ctagtcaaaa
     2761 taactagttc ctttccctcc ttcaccccct cctttagaag aatagtcccg acaccagcaa
     2821 gggattaatc tggccgtggc tcaatctggg agaaggatca tggcgacaca acaatacaag
     2881 gcttggtcgg tggtaggcat gagcacgctg tccttcacag tatgcttcat ggtgtggatg
     2941 atgtttgcgg tgatcggcat cccgatcaag gccacgctgg ggctgaacga aacccagttc
     3001 ggcatcctgg tggcaacgcc agtgctgacc ggctctctga tccgcctgcc gctgggcatg
     3061 tggaccgaca agttcggcgg ccgcgtcgtg ttcttcgtgc taatgctttc caccatcatc
     3121 ccgatctggt tcatttcccg ttgcactgaa ttctggcagt tcctggtcac tggcctgttc
     3181 gtcggcgtcg ccggcggatc gttcacggtg ggcatcgcct actgcgcacg ctggtttccc
     3241 aagaaccgcc agggactggc gatgggcatc ttcggcgccg gcaacactgg tgccgcagtc
     3301 accaagctgg tggcgccatc catcgtggtg gcctacggct gggccatggt gccgcaggta
     3361 tacgcagcca tcatgacggt caccgcacta ctgttctggt tcttcactta ctccgacccg
     3421 gcacacaagg tggacaaggc ggtgaccatg cgcgaacagc tggccacact caaggacccc
     3481 cgagtgtgga aatacagcca gtactactcc atcgtattcg gcggctacgt ggcgctggca
     3541 ctgtggatga ccaagtatta cgtcagcgaa tatggtttcg atcttaaaac tgcagcgcta
     3601 ctggcggctg ccttcagcat tcccggcggc gtattgcgcg ccatcggcgg tcatttttcc
     3661 gacaagttcg gcgcccacac cgtgacgtgg tgggtgctat gggtggcgct ggtttgcctg
     3721 ttctttctgt cctatccgca gaccgatgtt tccatcatga ccgttaaagg tccgcaaacc
     3781 tttcatatcg gtctctccgc tgcctggttc accatcatca tgttcaccat gggcatcgcc
     3841 ttcgccatcg gcaaggcctc ggtgttcaaa tacatctcgg atgactatcc gcacaacata
     3901 ggcgtcgtct ccggcgtggt cgggctggct ggcggcatgg gaggtttcct catgcccgtc
     3961 atgtttggcg cgctggtgga cgtcgccgtc gtgccctcct cgtctttcat gctgatgttt
     4021 ggcatcgtct gggtatcact gatctagatg tactggaccg aagtcaggcc ggtaaaaatc
     4081 gcccgccatc acgcacgcaa atttgctgac caactggaat aaggagccat catgtcgact
     4141 gaaatcaagg agtggaacat cgaggacgag aagttctggg aatccaccgg caagaaggtc
     4201 gcctaccgca acctgtggat ctcggtgccg gcgttgcttt gcggcttcgc cgtatggctg
     4261 atgtggggca tcatcacggt acagatgagc aatctgggct ttccgttcag caaggacgag
     4321 ctttttactt taacagccat ctccggcctg gcgggggcga ccatgcgcat cccgtcttct
     4381 ttcttcatac gcctggcggg cgggcgcaac accatcttcc tcaccaccgc catgctattg
     4441 agtccggcca tcggcaccgg catcgcgctg cagcatccgg aatggccgct gtgggtgttc
     4501 cagttgatgg cgctgtggtc cggcgtaggc ggcggtaact tcgccagttc catgtccaac
     4561 atcagcacat tcttccccaa gcgcctgcag ggcacggcgc tgggcatcaa cgccggactg
     4621 gggaatttcg gcgtcaccac catgcagatt ctgatcccgc tgctgatgac ggtcagcgtg
     4681 tttggcgcca tgggtggcga ctccatgttg ctggttaagg atagcggctg gattttcggc
     4741 aaaattcccg ccggtacgcc gaccttcatt cagaacgccg gctttgcctg gatgctgtcg
     4801 ctggtgccgc tggcgatcct gtgctggttc ggcatgaata atctgaagac catttcgcct
     4861 gacatcggcg gcaccatcgt ggccttcatc aagatcacct ggctctatac gctggctttc
     4921 gtaccggcca tcgccggcct ttacctttac ctgccggcgc cgtccggact cggactgctc
     4981 aatatgtggg tagcgatgcc gttgatcatc atggccaccc tgcttgtgat gaaactcgcc
     5041 gctttcggca ccatgaagga aaacattgcc aaacagttcg ctatcttcgg caacaagcac
     5101 acctggtcga tgacggcgct ctacctggtg accttcggtt cattcatcgg attttcgatg
     5161 gcgctgccgc tgtccattac cgtgatattc ggcgacaccc atacgctgga tgcggccacc
     5221 gggatgtgga cgcacgccaa gaacccgaac gctccctccg cgcttaccta tgcctggatc
     5281 ggccccttcg tgggcgccct gatccggccg gtgggcggct ggatttccga caaactgggc
     5341 ggttccatcg ttactcaggt gatttcggcg gtgatggtcg tggcatcgat ctacgccggc
     5401 cacatcatga tgcaggccta ccattccgcc acaccggaaa tctatttctc ccagtttttg
     5461 atcacattcg tcgtggtatt tgccgcggcc ggcgtcggca acggctccac tttccgcacc
     5521 gtgggcgtga tcttcgaccg cgtgcaggcc ggcccggtgt tgggctggac ttccgccgtc
     5581 gctgcctatg gctcgttcat cgccccggaa gtcatcaagg gccagatcaa ggcagggacg
     5641 ccggaaatgg cgatgtacgg tttcgcggtg ttctatgcgc tgtgcctgat attgaactgg
     5701 tggttctatc tgcgctccgg cgccgagatc aagaatccat aagccgataa caacgattta
     5761 cttaaggagt ctgctgtcat gagtcacttt ctggataggc tgaaattctt cgacaaggtc
     5821 aaggcgacct tctccaacaa ccacggtatc gttaccaacg aggacaggca gtgggaagac
     5881 gcttaccgtc atcgctggca gcacgacaag gtggtgcgct cgacccacgg cgtgaactgc
     5941 accggtggct gcagctggaa aattttcgtc aagaacggcc tggtgtcgtt cgagatgcag
     6001 cagaccgact acccgcgcac ccgtgacgac ctgccagacc atgagccgcg cggttgccag
     6061 cgtggcgcca gcttctcctg gtatctatac agcccgcacc gcgtcaagca tccgctgatt
     6121 cgcgggcgcc tgctcgacct gtaccgtgcc gagcgcaaga ccggcaagga tccggtggaa
     6181 gcctgggctg ccatccaggc tgacgagaag aagcgcaaat cctacacggc ggtgcgaggt
     6241 ctgggcggct tcgtgcgcag ttcgtgggac gaggtgaatg aaatcattgc tgccgccaat
     6301 gtccatacca tcaagaaatg ggggccagac cgtatattcg gcttctcccc gattcccgcg
     6361 atgtccatga tttcctacgc cgcgggctcc cggtatctct cccttatcgg cggtgcctgc
     6421 ggctcttttt acgactggta ctgcgacctg ccggctgcca gcccgcagac atggggcgag
     6481 cagaccgacg tgccggaatc ggcagactgg tacaactcga cctacatcat catcaccggc
     6541 gccaacctgc cgatgacgcg caccccggac gcgcatttcg ccgcagaagt gcgctacaaa
     6601 ggtgccaaga tcgtggcgat ggcccccgat tacgccgagt tctgcaaatt ctccgacctg
     6661 tggatgccga tccgccaagg caccgatacc gccgctttcc tggcaatggg acacgtggcg
     6721 ctgaaagagt tttatatcga aaaccaggat ccgtatttcc aggaatatgc ccgcaagtac
     6781 accgacctgc cgatgcaggt catgctgcgc aaacaaggtg atgcgtatgt atcagaccgt
     6841 ttcctgcgcg cctctgattt taacggggca ctgggcgaat ctaacaaccc ggactggaag
     6901 accattgtct atgacgaaaa atcccagtct ttcatcgcgc ccaacggttc gatcggtttc
     6961 cgctggggcg aggacggcaa atggaacctg ctggaaaaag gcggtgccag ccagcaggac
     7021 actacagcgc aactgagctg cattgacacg cgcgacgatg tggtgtcagt aggtttcccg
     7081 catttcaatc agggtgagga cgccctgctg ttccgcaacg tgccggtgcg caaggtcaag
     7141 ctggccaacg gcgaagaggc actggtcgcc accgtattcg acctgcagat cgcccagtac
     7201 ggtatcgatc gtgggctggg cggcgggaat gtagccagca actacgccga cgccaatgtg
     7261 gcctacacgc cggcttgggc ggagaaagtc actggtgtga aagctgccga catcgagcgt
     7321 accggccgcg agttcgccta taacgcctcc aggaccaagg gaaggtcgat ggtcatcatg
     7381 ggcgcggcca tcaaccactg gtaccacaac gatctggctt accgttccat catgaacttg
     7441 ctgcatatat gtggttgcgt cgggcagacc gggggagggt gggcgcacta cgtggggcag
     7501 gaaaagctgc ggccgcaagc cggctgggca ccgattgcgt tcgccacgga ttggcaccgt
     7561 ccgccgcgcc ataccaactc cacctcctac tggtacttcc acaccgacca gtggcgctac
     7621 gaaacgctca aggccgactc gctgctgtcg cctgccggaa aaggcaagaa caagggcaat
     7681 tcgctggccg actacaacgt caaggccacc cgcatgggct ggctgccttc catgcccaac
     7741 ttcaatcgca acccgatcga actggcggaa gaagcattgg caagcggcgc agccgacgaa
     7801 gctgcggtgg cgaaatacgt cgccgggcag atgaaatccg gcgcactgga tgtggcctat
     7861 gccgacccgg acaatccggt caactggcca cgcaatctgt tcgtctggcg tgccaacctg
     7921 atcggcactt ccgccaaggg ccacgaatac ttcctgaagc atttgctggg cgcccagaac
     7981 ggcgtattgc aggaatccgg cgccggtcgc gacaacaagg aagtcaagtg gcatgaagaa
     8041 gctccgatcg ccaaactgga tctgatggtg gacatcaact tccgcctcaa ttccaccggc
     8101 gcctattcgg atatcgtgct gccgaccgcc acctggtacg agaagaacga cctcaacacc
     8161 accgacatgc accccttcat ccacccgttg tcggaagccg tttctccggg ttgggagtcg
     8221 aagtccgact ggcagatttt caagaccatc gccaagacct tctctacctt ggccgaaaaa
     8281 cacctgggga cgaagaagga agtcgtcacc ctgccgatgt tgcacgactc ggccgcagaa
     8341 atggcccagc cgttcggcga ggtgaagaac tggaaagaag gcgactgcga cccgattccc
     8401 ggcaagacca tggcgatcat caaactggtc gagcgcactt acggcgacac ctaccgcaag
     8461 tacatcgcgc tggggccttt gatgcaaaag cttggcaata acatcaaggg tatcgactgg
     8521 aacaccgacc aggaatacga ggaactcaag aagcacaacg ccacggtgaa agaagccggg
     8581 gtgtctttcg gcatgccgtc gctggaagaa gatatctacg tatgcgactc ggtgatgcgc
     8641 atggcgccgg aaaccaacgg cgaagtggcg cacaagtcat ggaactcgct gtcgatcaag
     8701 accggcattg atcaccacca cctgtatgcc gggcgccacg aggacaagat cacattcaag
     8761 gacatccagg cccagccgcg caaaatcatc acggcgccga cctggtccgg gatcgagtcc
     8821 gagaccgtat cctacaccgc cggctacacc aacatccacg agcacatccc gttccgtacg
     8881 ctcaccggcc gtgcgcactt ctatcaggac cacgaatgga tgctggattt cggtgagggc
     8941 ttctgcactt acaaaggaat ggtcgatctg ggcgaactca actcgttgcc ggccaatgtg
     9001 atcaacaagc cgcatctgga ggtgaactgg atcacgccgc actccaagtg gggcatccat
     9061 tccagctatc aggacaccct gcgcatgctg acgctgttcc gcggtggccc ctatgtctgg
     9121 atctccgaaa ccgacgccaa gtccatcgga ctggaagaca acgactgggt ggaagcggtc
     9181 aatcacaacg gcgcaaccgt ggcacgagcc gtagtttcgc agcgtgtccc gcagggcatg
     9241 gcgatgatgt accacgccca ggaaaagaac gtgaacgtgc ccggatcgcc atccacaggc
     9301 aagcgtggcg gcatcctcaa tgcggtgacc cgcgtcatca tcaagccgac caacatgatc
     9361 ggcggctacg cgcaactggc ctacggcttc aactactacg gcacggtagg ctgccagcgc
     9421 gacaccatgg tggccatcca caagatcgcg gacaaggatg tggactggct ggagcgtccg
     9481 ctgacgccgg aacgcgaagc gcaactcaac ccggtcggca tcggcccgcg ctaacaggac
     9541 acaggagaac atcatgaaag tacgcgcaca attcgccttt gtcttcaacc tggacaaatg
     9601 catcggctgc cacacctgtt cggtcacctg caagaatgtg tggaccaacc gcaagggcgt
     9661 ggaatacgcc tggttcaaca acgtcgaatc caagcccggc atcggctatc ccaagcagtg
     9721 ggaaaaccag gaggtgtgga acggcggctg ggaactcaag aacggcaagc tggagctgaa
     9781 atccggtggc cgcgttaaca aactgcttaa catcttcgcc aatccggacc tgccggaaat
     9841 cgacgactat tacgagccgt tcacctataa ctacgcgcac ctgcaaaatg cgccgctgtc
     9901 ggaagctaca cccaccgctc gccctatctc gcagattacc ggcgagagca tggagaaaat
     9961 cacctggggt ccgaactggg aagatgactt ggccggagag ttccacaagc gatccaagga
    10021 caagaacctg gacaacatcc agaaggagat gtacagccag ttcgagaaca ccttccacat
    10081 gtacctgccg cgcatctgca accactgtct caacgccgcc tgcgtggcgg cctgtccgtc
    10141 tggctctatc tacaaacggg aagaagacgg catcgtgctg gtggatcagg acaagtgccg
    10201 cggctggcgc atgtgcgtca gctcctgccc ctacaagaag gtgttttaca actgggagtc
    10261 gggcaaggcc gagaagtgta tcggttgcta cccgcgtgtg gaatcgggca tgccgacggt
    10321 atgttcagaa tcctgtgtgg gccgcatccg ctacaacggc atcatgctgt atgacgccga
    10381 tcgcatcgag gaactggcca gcatcgagaa cgagcaagac ctgtatgaag cccagtgcag
    10441 gattttcctt gatcccaaca atccggaagt aattgccgcc gcccgcgcgg aaggcatcaa
    10501 cgacgactgg ctcgaggctg cgcgtaaatc gccgatctgg aagatggcca tggactggaa
    10561 gatcgccttc cccatgcacc cggaattccg cacactgcct atggtgtggt acgtgccgcc
    10621 gctgtcgccg gtacagtcgc agatcgacca aggcaacctg ccggtcggac cggatggcgc
    10681 gattccggtc gccggcacga tgcgcctgcc ggtgcagtac ctggccaatc tgctcaccgc
    10741 gggaaaaacg gcacccatcg aaagcgcact caaccggctg atcgcgatgc gcagttacca
    10801 gcgctccgtg catgtggaag gcaaggcaga tacccgtgcc atcgatgcgg ctggcattac
    10861 cgaagatcag gcgaaggaga tgtaccgtta cctcgccatc gccaattacg aagaccgttt
    10921 cgtcatcccc accggccata cggaagagac attgaacgac gcctatggct tccagggaca
    10981 gaacggcttc accttcggca acgacagttc atcgggcgtt tccaagatta cgttgttccc
    11041 gcgccgccgc aaggaaacgg tggaaccaaa agagcttgcc ccgtctggtg acaatcgtta
    11101 ggagatcatg atgcgactgt acaaaatgtt atccgtgctg ctggagtacc cagatcagga
    11161 actgctcgac aacctgcctg aactgcgcag cgaaattgac gcctgtatcg atatggatga
    11221 tgccgaacgc agtgctatgc ggcactttct aatacacctc gaacagacct cgcttaccga
    11281 tctgcaggcc gattacgtca agactttcga cctcacggca gagaacagcc tgcacctgac
    11341 ccaccacttg ttcggtgatg acaagaaccg cggcccggcg ctgatcgacc tgggtgaaat
    11401 gtacaaggat tacggcgtgg aggtggtgac caacgagttg ccagactatc tgccgctgat
    11461 cctggaattc gctgcctatc tggatgacag cgaggccagt gtattcctgg ccgacgctaa
    11521 caaggtgctg ggggtactgg ctgacaacct gaaacaagcg ggcagtccct acgcgggact
    11581 tttatttatt gtcgaagggc gggccagcct gacccgtctt gcagcctgag gagggactaa
    11641 ccatgaacct gcacaacttt ctctacgggg tctatcccta catcgcgctc agcgcatttc
    11701 ttctgggcag ctggctgcgc ttcgaccgtg agcagtacac ctggaaaagc gattcgagcc
    11761 agttgctgtc caaggccgga atgcggctgg gcagcaatct gttccatatc ggcatccttg
    11821 ccatcttcgg cggccatctg gtcggtctgc tgacgccgca cagtgtattc cgctcactgg
    11881 gggtgtcgga catgaaccac cagatgatcg ccatctgggc cggctcgatt ttcgggctgc
    11941 tctgcctggc cggcggcgcc atcctgtggc tgcggcgcat gaccaatgcc agggtgtcgg
    12001 ccgctagccg gggctcggac aagttcatcc tgacctggct gctgctgacg ctgatgctgg
    12061 ggctgtccac catccccgtt tccatcggcc atgccaacga aggcaacccc ggcgtcatga
    12121 tcgcactggc ggaatgggta cagagcatca tttacctgca ccccaacccg gcgctgctgg
    12181 acggcgtgga taccatcttc aaagtgcacc tgtttttcgg catgacggta ttcctggtgt
    12241 tcccgttcac ccgcatggtg catgtgtgga gcgcgccttt cggctatctg gggcggcctt
    12301 accaggtcgt gcgcagcaaa cgttatcact aagctcaccc atgcgcgacg tttcaaaatt
    12361 cctgtcgggc ttcaggcgct tccagcatca ctacctgggc gcacgccgcg cgtattttga
    12421 ccgcctgatc aaggaaggtc agcatcccac ggcgcttgtc gtggcctgct gtgattcgcg
    12481 ctgcgacccg gcgctgctga ccgattgcga tccgggcgac atgttcgtgg tacgtaacgt
    12541 cgccaatctg gtgccaccct atacccaagc cggttcgttt gccgccacca gttcagccat
    12601 tgcatttgcc gtccaccaac tggaagtcga acatgtcatt gtcatggggc acgcccagtg
    12661 cggcggtatc cgcaccctga tcgaacacaa gccggtggca tgcgatgaag accagcttat
    12721 cgccaaatgg ctgggcatcg ccgaggatgc acgccagaaa gccatgcagg cgctacccgg
    12781 caggacttcg gaaacacagg cacgggcctg tgaacaggcc tccattctgg tctcgctgga
    12841 aaacctgatg tcctacccct ggatcaaaag ccgcgtagaa gcgggaaaac tgctgctgct
    12901 cggctggtat ttcgacatgg tcaacggcga actgctgcaa tacgaagcgg atcccgggga
    12961 gttccgcgcg ctggtctcgc atgagggcat tggcaaaagc accgaacatc tgtggtggag
    13021 ggcaccgggt tgaagccaac aacctctatc agccttgccc agattcggga gcctgcgcgg
    13081 ccgcgcccct ccgattccgg tggccgctgg cggcatttca ctgccatgcc gcaccacgtg
    13141 ctgttcgcca tcggcgcagt acaggcggtg ctggtcatgc tgtggtggct ggttgacctg
    13201 ggcgggcgct atggcggctg gtatgccccg atcgactgga cgctgccatc tccttgggcg
    13261 cacctgtatt tgatggtcta ctgcctgttc ccgccttata tgttcggttt cctgatgacg
    13321 acctacccga aatggatggc gggggtgccg gtggcgcgca aacactatct ggcaacggca
    13381 gcgttgctgg ggacgtccgc cctgctgggc tatgtcggcc tggcaacaga aaaatcctgg
    13441 ctgctgctgg ccatggggct gcatctggcc ggctgggcca cgggcatcta tgccctgctg
    13501 cgcgtttatg ccggcgccca gcgccccgat accttgcatg cgctgattac cagcggcgtc
    13561 ctgctgctcg gctggggcct gctactgacc tacatctcag gggtgcaaac gggtaatgct
    13621 ggtctggtcg ccatcgcccg tagcggcggc atctggtggt tcctgctccc ggtatttttc
    13681 tccgtcagcc atcgcttgat ccccttcttt tccagcgtgg tgctgccgga ttaccagatc
    13741 atccgccccg actggacgct gtggctgatg agcgccggca gcctggtgca cggcacactg
    13801 gaattgctcg ggcaggaaca gtggacatgt ctggtcgatc tgccgatggc cgtcgtggca
    13861 gcctggctgt cctacgtctg gtgcttccgg aaaagttttc agacgcgcat cttggccatg
    13921 ctgcacatgg ctttcgcctg gctgagtgtc gcactggccc tgtacggcct gcaaagtctg
    13981 agcttgccac tgggccgcgc gccgctgcac gccctggtgg tgggctactt cgcctccatg
    14041 ctggtggcaa tggccacgcg cgtcacactg gggcattccg gccgcccgct gacggccgac
    14101 aacctggcct ggggtatctt tctgatggtg caggctgctg ctctgctgcg ggtgctggcg
    14161 gatttgcccg tcatcaacgc ctacgccgcc cacctctacc tctgttccgc attactgtgg
    14221 ctggctgcct ttatcagctg gctaatcaaa ttcgcaccga tttactggcg accccagtct
    14281 acatgattca tcacttcagg agaccattca tgaaaaacgc cacaaaactg cttgcactag
    14341 ccgtgttatc cgcccttggt cccactgcgg cccgagccga ggacgcaccg gcggcaccgc
    14401 ccgcctcgga atacaccttt ctggactcga
//
LOCUS       DAOMFA010000022        14393 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_361_length_14393_cov_5.130353, whole genome shotgun sequence.
ACCESSION   DAOMFA010000022 DAOMFA010000000
VERSION     DAOMFA010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 14393)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14393)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14393
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_361_length_14393_cov_5.130353"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..243)
                     /locus_tag="PKW44_02135"
     CDS             complement(<1..243)
                     /locus_tag="PKW44_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018292295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HQR50419.1"
                     /translation="MHPTLKNIVNATSQVILGKDRQIRLALACVLARGHLLIEDLPGM
                     GKTTLAHILAQVLGLEFRRVQFTSDLLQADILGVSVY"
     gene            complement(243..1712)
                     /locus_tag="PKW44_02140"
     CDS             complement(243..1712)
                     /locus_tag="PKW44_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HQR50420.1"
                     /translation="MTPLYLTSQIRTMEQAHGSAGLMEKAGLAAAELSRALLSEEADA
                     LLVLAGPGNNGGDALVAARHLRHDWHRVDVVFTGSADKLPADASDAYDAWLACGGEVL
                     AAIPAGKGYGLIIDGLFGIGLTRELDVRHRDIVRQVNAMDVTVLALDVPSGLDADTGR
                     ILGCAIKADHTLAFLGLKPGLFTLDGPDHAGLVHVTDLGIEADPSGAQPGWLIDTPPT
                     LPAPRRRNSHKGSYGSAGILGGDTAMVGAALLAGRAALLAGAGRVYAGLLAEHAPVVD
                     FGQPELMLRSAGTLFDIAPFGVLAAGPGMGRSQRAEAALLRAIHTPAPLLLDADALHL
                     IAANDQLRDQFHQRTHGNILTPHPGEAAALLGCSVAEIQADRVASALDIAQACNAITV
                     LKGCGSVIATPDGRWFVNASGNAGLASAGMGDVLSGLIAALVAQDMTPEAATLLGVYL
                     HGAAADSLVGAGVGPVGLTASEVAQEARDLLNQWIYGTR"
     gene            complement(1741..2883)
                     /locus_tag="PKW44_02145"
     CDS             complement(1741..2883)
                     /locus_tag="PKW44_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQR50421.1"
                     /translation="MMVSEHATLRWVENGEPRSARWRSESAAPPPRRVIIADDTIMAD
                     AAYRLACEGTALLWRGDFHNARQLLQALARRIERRPRKAKAEKPAASLTEAFHLYRQS
                     QAQRARTLGMLLLPFEPDHSIALRRAPDARQACLEAYGPTEEPYVASLRELQGLVGAH
                     EWRKKGVPIAALDAHIHPHYGVFSPVRGEYVALVAEAPLPSTELAFDIGTGTGVLAAV
                     LARRGVKRVVATDQDARALACARDNIERLGLAEQVEVVQADLFPEGRAPLVVCNPPWL
                     PGRPSSALEYAVYDPESRMLRGFLGGLAAHLTPQGEGWLILSDIAEHLGLRSREELLA
                     MFEQAGLKVLDRTEVHPHHPRAADTSDPLHKARAAELTSLWRLAAR"
     gene            complement(2870..3130)
                     /locus_tag="PKW44_02150"
     CDS             complement(2870..3130)
                     /locus_tag="PKW44_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR50422.1"
                     /translation="MRSTRASSAVTRLNRRSNGREYTMAVTGAGLFSLSEPTAAGEQR
                     VFDPVPLDEFVRLVDAMGPQQIRRMTKNDVAFAKQLKRHDGE"
     gene            complement(3190..4095)
                     /locus_tag="PKW44_02155"
     CDS             complement(3190..4095)
                     /locus_tag="PKW44_02155"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HQR50423.1"
                     /translation="MDRLLAMRVFATVVESGSFTRAAEQLHLSATATSRHVAELEKHL
                     GAQLLQRSTRRLHLTEIGTNYYDRCRTILADVEEAEAQAATSESQPRGILRISLPHSF
                     GLRYIAPLMPEFCKRYPELQLEFNFSDRRVDLVEEGIDMAVRITGDLKTSLVARKLAP
                     VRIACCAAPAYLQRFGTPQTPEDLAQHDCISYSYAPTANAWTFLRDGKPYDVPIKGRL
                     RSNSGDMGRLAALDGLGITTLPVFIVCDDLRAGTLTELLTEHPIPPINVYAVYLPGAR
                     RAARIKAMVEYLWDALGQGNPPWQQ"
     gene            4173..5006
                     /locus_tag="PKW44_02160"
     CDS             4173..5006
                     /locus_tag="PKW44_02160"
                     /EC_number="1.13.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018413250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class III extradiol ring-cleavage dioxygenase"
                     /protein_id="HQR50424.1"
                     /translation="MKTQAETRRSLPALFVPHGAPTFALQPGEAGAALAHLSHQLSEV
                     KAVLLISAHWDTEQPTLGVASRPDTIHDFYGFPQALYSIRYPAPGAVPWAMEARTLLE
                     ESGYEVKLDPLRGLDHGAWIPLRLMFPDAGVPVFTLSLQSHLGPEHHYRIGQALAPLR
                     EAGVLIVGSGNLTHNLMHFGLLRGASHAPAYVTDFQGWVRDKLAAGDAASLLDYRALA
                     PGAEVAHPTDEHLLPLYVALGAAGADFAAEQVYSGIEHNMLAMDAYTFRSGADFQTHS
                     H"
     gene            5018..5257
                     /locus_tag="PKW44_02165"
     CDS             5018..5257
                     /locus_tag="PKW44_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0106034 - protein maturation by [2Fe-2S]
                     cluster transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HQR50425.1"
                     /translation="MSEPVIAQKSPYAVDVEAGKSYWWCSCGKSKSQPFCDGSHKGSE
                     FGPVEYKAEKSGTVYFCGCKHSANGVLCDGSHSRL"
     gene            5267..5965
                     /locus_tag="PKW44_02170"
     CDS             5267..5965
                     /locus_tag="PKW44_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018077213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HQR50426.1"
                     /translation="MIQLRKSNARGHADHGWLNARHTFSFADYYDPAFMGYSVLRVIN
                     DDEIAPGRGFGTHPHRDMEIITYILDGALQHRDSMGNGSVIRAGDVQYMSAGSGVRHS
                     EVNPSPDAAVHLLQIWILPAQPGLVPQYDQKTIAAEQKRGRWRMLASPDGREDSITIR
                     QDAMLLATMLDSDADLTYRSQPGRRQYLHLARGTAALNNVAMRGGDGAFIENENLLTL
                     SDAKGAEALLFDLP"
     gene            5981..6565
                     /locus_tag="PKW44_02175"
     CDS             5981..6565
                     /locus_tag="PKW44_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HQR50427.1"
                     /translation="MKKTLIALALAAWIPAALADAETYNIDNSHSFANFSIRHVVSRL
                     SGTFPDVTGKIVIDRADLSKSSVDARINVLSINTSLAKRDEHLRDKPEYLEAGKFGQM
                     TFVSTKVEARGKDEGVLTGKFTLHGVTKEISFPFKVLGFGADPWGGERSGFEAHTKLN
                     AADYGFGWAANPNGPVGTEIDVTLLIEGVKEKAK"
     gene            6562..7638
                     /locus_tag="PKW44_02180"
     CDS             6562..7638
                     /locus_tag="PKW44_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkene reductase"
                     /protein_id="HQR50428.1"
                     /translation="MSLDLFSPVKLGRTELKNRIVMAPLTRNRAAMPGNLPQDINVTY
                     YAQRASAGLIVTEATPISAMAHGYPALPGIYSSEQVAGWKKVVDAVHARGGKIFMQLW
                     HVGRISHPSLLPDNALPVAPSAIKPAGQAFTYAGLQDYVTPRALATDEIPTLLDKYRT
                     AAQNALQAGFDGVEVHAANGYLIDQFLRDGTNQRTDQYGGSIANRTRLLQEVVATVSK
                     VWGSDRVGVRLSPINPFNDICDSYPQALFNHVAEALNPFNLAYLHVVEIGMGGDTPQP
                     FDFAELRRAYHGNYMANGAYDKARGNAAIASGHADCVAYGVPFIANPDLVERFAKDAP
                     LNIADQNTFYGGGEQGYTDYPALA"
     gene            7644..7919
                     /locus_tag="PKW44_02185"
     CDS             7644..7919
                     /locus_tag="PKW44_02185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR50429.1"
                     /translation="MSGPVSYQLAIMLDQPARLTVGRLGVFDFPAGRYVYTGSARRSL
                     EARIARHLLERDAAQVVRVESDHDGILADVDIPKDLERFTINFQEQP"
     gene            7916..8515
                     /locus_tag="PKW44_02190"
     CDS             7916..8515
                     /locus_tag="PKW44_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HQR50430.1"
                     /translation="MKKPAITQVPIQDVIANRWSGRAYDPAKPVSCDQVIALLEAARW
                     APSCYGDQPWRFVVWNKGEDAAAWQAAFETLVPGNQAWVKDAPLLLLVCADSLFNHNG
                     QPNRWAQYDTGAAAENLCLQAETLGLMAHQMGGFDPDRARALVGVPEQYSLMAMVSVG
                     YPADPATLGAEVLARETSPRKRRELGELFFEAVWNKPVA"
     gene            complement(8530..8979)
                     /locus_tag="PKW44_02195"
     CDS             complement(8530..8979)
                     /locus_tag="PKW44_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020182250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR50431.1"
                     /translation="MEPTKQRRWARWLIPLAIAPLVLFTLYTWLVLNWSYSSGERAGY
                     VQQFSKKGWLCKTWEGELAMVTMPGTLTEKFRFTIRDDAVAAKINQTLGKKVSLTYEQ
                     HLGVPTSCFGDTEYFVADVRLIQEELHINNKAGAANAEPAPVVPPTP"
     gene            9076..9444
                     /locus_tag="PKW44_02200"
     CDS             9076..9444
                     /locus_tag="PKW44_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HQR50432.1"
                     /translation="MAVIELTKANFKQTIENNALVIVDFWAPWCDPCLTFTDTFRQVA
                     DHHPDIVFGMVNTETDNEIAEYFNVEQIPGILVVREQAGLLAQVGELGAPALEEIIKW
                     ARDYDMSTVHEYYAGQQKQA"
     gene            complement(9616..9951)
                     /locus_tag="PKW44_02205"
     CDS             complement(9616..9951)
                     /locus_tag="PKW44_02205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR50433.1"
                     /translation="MNNRSPDSLTSLLVALGQLILLLLGIVGISVHLFRPDGWLSQAV
                     GHVFETWSFTAIAAIPLLAITYLIVDKWYANNFKDTQEKVANLMLFAMMGVGAFFLFK
                     LVSTGSFTG"
     gene            complement(9988..11010)
                     /locus_tag="PKW44_02210"
     CDS             complement(9988..11010)
                     /locus_tag="PKW44_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HQR50434.1"
                     /translation="MKHLLPFIVAVLLAGCASSQKAPVIQRTPEARKPAPAKPAAAEA
                     PASKSAAPEKDWRPETYTVKKGDTLYSVGLEFGFDYKEIAQWNDIQPPYVIRIGQKLK
                     LKESTKATDDQASQDTVATPLKTEPLPAVKPLTDEVPAAKPATDSTATAKPAGEPPLL
                     TEPKALKEPYSEKAMAAAPPKPAATVAASEKPAEPVKPEATKPETPAETVSPVIEEGE
                     VANWAWPAKGKVLAGFNDAASAKGVDIAGTQGQPVYAAAGGKVVYSGSGLRGYGKLVI
                     IKHNKTYLSAYAHNSQILVKEGQEVAKGQKIAEMGSTDTDRVKLHFEIRKQGKPVDPT
                     KYLPEG"
     gene            complement(11007..11672)
                     /locus_tag="PKW44_02215"
     CDS             complement(11007..11672)
                     /locus_tag="PKW44_02215"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HQR50435.1"
                     /translation="MALMNQNRSGIGMTSQRTRERMVARLREQGIRDEVVLAAMNTVP
                     RHIFVDEALSIRAYEDVSLPIGYGQTISQPYIVARMTEILRGGNQLGKVLEIGTGCGY
                     QTAVLATVSQETYSVERISPLLMKARGHLRELRARNVKLKHADGAMGLAEVAPFDAIM
                     VTAGATHIPEELLHQLAVGGRMVIPVGATEQTLVLIERTPEEYRKTKLEAVKFVPLLG
                     GTA"
     gene            complement(11660..12400)
                     /gene="surE"
                     /locus_tag="PKW44_02220"
     CDS             complement(11660..12400)
                     /gene="surE"
                     /locus_tag="PKW44_02220"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019899254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HQR50436.1"
                     /translation="MRILLSNDDGYFALGLSILAEALKPLAEIVVVATERNRSGASNS
                     LTLDRPLTVRQAANGFYFVNGTPTDCVHLAVTGLLDQLPDMVISGINDGANMGDDTIY
                     SGTVAAAMEGYLLDIPSIAVSMSQHGAQHFDTAARVVVDLVRRFEKNAFPPPVLLNVN
                     VPDIPYEQVSGMRITRLGKRHKAEAVVKSSTPRGETVYWVGAAGAAQDAGEGTDFHAV
                     EQGLVAITPLQVDLTRYGKLDALKSWLS"
     gene            complement(12417..12752)
                     /locus_tag="PKW44_02225"
     CDS             complement(12417..12752)
                     /locus_tag="PKW44_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2069 domain-containing protein"
                     /protein_id="HQR50437.1"
                     /translation="MIRTLQLATSVSLVALILLCLAWEGWLAPLRPGGSLLILKAVPL
                     LLPLFGILRGRRYTYQWACMFILLYFTEGIMRAWSDNGLSSRLALIEIVLSLTFFVSA
                     IFYARMTRR"
     gene            complement(12796..13521)
                     /locus_tag="PKW44_02230"
     CDS             complement(12796..13521)
                     /locus_tag="PKW44_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2470 domain-containing protein"
                     /protein_id="HQR50438.1"
                     /translation="MSAGAAVEARKFLRATRSAVLSTHSARFSGYPFGSIAPFVLDHD
                     GNPLILISTIAEHTKNIQKNPHVSLIAFDPAATDMQAGARLTLLGEATIADKQDGTLR
                     ARYLAYFPQAAQYFDMHDFLFHRIEIRQARYIGGFGNIHWISGEELRPPANMLAEQEA
                     GILAHMNTDHADSLRAYCRHVHRIDAENAEMVGIDTDGFDLRAGEKLLRFTFDAPVTD
                     AQSARAALVAMSKAVVGAAPSPR"
     gene            complement(13518..14108)
                     /gene="wrbA"
                     /locus_tag="PKW44_02235"
     CDS             complement(13518..14108)
                     /gene="wrbA"
                     /locus_tag="PKW44_02235"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020182507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HQR50439.1"
                     /translation="MADVLVLYYSHSGSVREMAQLVARGVEMVDGAKARIRTVPRVST
                     VCEATEPDIPANGAPYVELQDLEQCAGLALGSPTRFGNMAAPLKYFLDGTSSLWLKGA
                     LAGKPAAVFTSTASMHGGNEATLLSMMIPLLHHGMVIVGLPYSEPELTSTTGGGTPYG
                     ASHVAGATDDQPISEAEKTLCIALGKRLAKMALKLT"
     gene            complement(14108..>14393)
                     /locus_tag="PKW44_02240"
     CDS             complement(14108..>14393)
                     /locus_tag="PKW44_02240"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008452901.1"
                     /note="Catalyzes two discrete reactions in the de novo
                     synthesis of purines: the cleavage of adenylosuccinate and
                     succinylaminoimidazole carboxamide ribotide; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HQR50440.1"
                     /translation="GISKLEANDAAMAADLDANWEVLAEPIQTVMRRYGVENPYEQLK
                     ALTRGKGGITKEALHEFIATLAIPQEARNQLLAMTPASYVGKAAELAKRI"
BASE COUNT         2588 a         4403 c         4633 g         2769 t
ORIGIN      
        1 gtataccgag acgccgagta tgtcggcctg cagcaggtcg ctggtgaact gcacgcggcg
       61 gaattccagc cccagtacct gcgccaggat gtgcgccagc gtggtcttgc ccatgcccgg
      121 cagatcctca atcagcaggt gcccgcgggc caggacacag gccagcgcca ggcggatttg
      181 ccgatccttg ccgaggatga cctggctggt ggcgttaacg atgtttttca gggttgggtg
      241 cattagcggg ttccatagat ccattggttg agcagatcgc gcgcttcctg cgcaacttcc
      301 gaggcggtga ggccgacggg gcccacgccc gcgccaacca ggctatccgc cgccgcaccg
      361 tgcaggtaga cgccgagcag agtggcggct tccggcgtca tgtcctgcgc aacgagagcg
      421 gcaatgaggc cgctcaacac gtcgcccatg ccggcgctgg cgaggccggc gttgccgctg
      481 gcgttgacga accagcggcc gtccggcgtg gcgatgacgc tgccgcagcc tttcagcacg
      541 gtgatggcgt tgcaggcttg ggcgatgtcg agcgcgctgg cgacgcggtc ggcctggatt
      601 tcggcgacgg aacagccaag cagcgccgct gcttcgccgg ggtggggggt aaggatgttg
      661 ccgtgcgtac gctggtggaa ctggtcgcgc agttggtcat tggcggcgat caggtgcagt
      721 gcatcggcgt ccagcagtag cggcgcgggg gtgtggatgg cgcgcagcaa ggccgcttca
      781 gcgcgctggc tgcgccccat gccggggccg gcggccagca cgccgaaagg ggcaatgtcg
      841 aatagcgtcc cggcgctgcg cagcatcagt tccggctggc cgaaatccac gaccggcgcg
      901 tgctcggcca gcagtccggc atagacccgc ccggcacccg ccaaaagagc ggcgcgcccc
      961 gccagcagcg cggcgccgac catggcggtg tcgccgccga ggatgccggc cgagccgtag
     1021 ctgcctttgt gggaattcct tcggcgcggg gcgggcaggg tgggtggagt gtcgatcagc
     1081 cagccgggct gtgctccgga tgggtcggct tcgatgccga ggtcggtgac atgcaccagc
     1141 cccgcatgat ccggcccgtc cagcgtgaac aggccgggct tcaacccaag gaaggccagc
     1201 gtgtggtcgg ccttgatggc gcaacccagt atccggccag tgtcggcatc caggccgctg
     1261 gggacgtcca gcgccagcac ggtgacatcc atggcattga cctgccgtac gatgtcccga
     1321 tgcctgacat ccagttcgcg cgtcaggccg atgccgaaca ggccgtcgat gatcagtccg
     1381 taacccttgc ctgccgggat ggcagccagc acctcaccgc cacaggccag ccaggcatcg
     1441 taggcgtcgc tcgcatccgc cggcagtttg tcggcgctac cggtgaatac cacgtccacg
     1501 cggtgccagt catgccgcag gtggcgtgcg gcgaccaggg catcgccgcc gttgttgccg
     1561 gggccggcca gcaccaggag ggcatcggcc tcttcggaca gcagcgcgcg cgacagttct
     1621 gccgcggcaa gaccagcctt ttccatcagg ccggcgctgc catgtgcctg ttccatggtg
     1681 cggatttggc tggtgaggta gagcggggtc atggtgcaag tttatcgcaa accatccaga
     1741 tcagcgtgcc gcaagtcgcc acagcgaggt cagttccgcc gcgcgcgcct tgtggagggg
     1801 gtcgctggtg tcagcggcgc gcgggtggtg tggatgcacc tccgtccggt ccagcacttt
     1861 cagcccggcc tgctcgaaca tggccagcag ttcttccctg gagcgcagtc ccaggtgctc
     1921 ggcaatatcc gacaggatca gccagccttc accctgcggc gtcaggtgtg cggccagccc
     1981 gccgagaaag ccgcgcagca tgcggctttc cgggtcgtaa accgcatatt ccagcgccga
     2041 gcttggccgt cccggcagcc agggcgggtt gcataccacc agcggcgccc ggccttccgg
     2101 gaacaggtcg gcctgcacta cttccacctg ctcggccagt ccaaggcgtt caatgttgtc
     2161 gcgggcgcag gccagcgcgc gagcgtcctg atcggtggcc accacacgct tgacgccgcg
     2221 gcgcgccagc accgcggcca gcacgccggt gccggtgccg atgtcgaagg ccagttctgt
     2281 cgaaggcagg ggggcttcgg ccacaagcgc cacgtattcg ccgcggactg gcgagaacac
     2341 gccgtagtgt gggtggatgt gcgcgtccag cgcggcgatc ggcacgcctt tcttgcgcca
     2401 ttcgtgcgcg ccgaccagcc cttgcagttc acgcagcgag gcgacatagg gttcttccgt
     2461 cgggccgtag gcttccaggc aggcctgtct ggcatcggga gcgcgtcgca gggcaatgct
     2521 gtggtcaggt tcgaacggca atagcagcat ccccagtgtg cgagcgcgtt gtgcctgcga
     2581 ttgtcgataa aggtgaaacg cctcggtgag cgaggcggct ggtttctcag cctttgcttt
     2641 gcgcggcctg cgctcgatgc gtcttgccag tgcctgcagc agttggcgcg cgttgtggaa
     2701 gtcgccgcgc catagcagcg ctgtgccttc gcaggccaat cgataggcgg catccgccat
     2761 gatggtgtcg tcggcgatga tcacgcgcct gggtgggggt gcggcgcttt ccgagcgcca
     2821 gcgtgccgag cggggctcgc cgttctcgac ccagcggagg gtggcgtgct cactcaccat
     2881 catggcgctt cagttgcttg gcgaaggcga catcattctt ggtcatacgg cgtatttgct
     2941 gcggccccat ggcatccacc aggcgcacaa attcatcaag cggcacgggg tcgaaaaccc
     3001 gctgctcgcc cgctgccgtg ggttcgctca ggctgaacag cccggcgccg gtgacggcca
     3061 tggtgtattc gcggccattg ctgcgccggt tgagccgggt gacggcagaa gaggcgcggg
     3121 tcgagcgcat gtgaaattcc ttaaagggag gaagcgtagc ctatacaact cgccaagtgc
     3181 agacaagctt tactgttgcc atggcggatt gccttgcccc agtgcgtccc acaggtattc
     3241 caccatcgcc ttgatgcgcg ccgcgcgacg ggcgccgggc aggtagacgg catagacgtt
     3301 gatcggcggg atgggatgtt cggtcagcag ttccgtcagg gtgccggcac gcaggtcgtc
     3361 gcagacgatg aacaccggca gcgtggtgat gccgaggccg tcgagcgctg ccaggcgccc
     3421 catgtcgccg ctgttggagc gcaggcgacc cttgatcggc acatcgtagg gcttgccgtc
     3481 gcgcaggaat gtccaggcgt tggcggtcgg cgcgtagctg taggagatgc agtcgtgctg
     3541 cgccaggtct tccggcgttt gcggcgtccc gaagcgttgc agataagccg gtgcggcgca
     3601 gcaggcgatg cggaccggcg ccagcttgcg cgcgaccagg ctggttttga ggtcgccggt
     3661 gatgcgcact gccatgtcga tgccttcttc caccagatcg accctgcgat cggaaaagtt
     3721 gaattccagt tgcagttccg ggtagcgctt gcagaattca ggcatcagcg gcgcgatgta
     3781 gcgcaggccg aagctgtgcg gcagactgat gcgcaatatt ccgcgcggct ggctttccga
     3841 cgtggcggcc tgggcctctg cttcttccac gtcggccagg atggtgcgac aacggtcgta
     3901 atagttggtg ccgatttcgg tgaggtgcag acgccgcgtg gagcgctgca gcagctgcgc
     3961 tcccaggtgc ttttccaact ctgccacgtg gcgcgaggtg gcggtggcgg aaaggtgcag
     4021 ttgctcggct gcgcgggtaa acgagccgga ttccaccacg gtggcaaaaa cgcgcatggc
     4081 caggagtcta tccattattc cgatatacgg gataaataat gtagaaatca catatttatc
     4141 ccgaacaaag aaattgtcaa actggcgcca tcatgaaaac ccaagctgaa acccgtcgat
     4201 cactgcctgc gctgttcgtg ccgcacggcg cgccgacctt cgccctgcaa ccgggcgagg
     4261 ccggggcagc gctggcgcac ctctcgcatc aactcagcga ggtgaaggcg gtgctgctga
     4321 tctcggcaca ctgggatacc gagcagccga cgctcggtgt cgcctcgcgc cccgacacca
     4381 tccacgattt ctacggtttt ccgcaggcgc tgtattcgat ccgctacccg gcgcccggtg
     4441 cggtgccatg ggcgatggag gcacggacgc tgctggaaga atcaggatat gaagtcaagc
     4501 tcgacccgct gcgcgggctc gaccacggcg cctggattcc gctgcggctg atgtttcccg
     4561 atgccggggt gccggtgttc acgctttcgc tgcaaagcca tttaggcccc gagcaccatt
     4621 accgcatcgg ccaggcgctg gcgccgttgc gcgaggccgg cgtgctgatt gtcggctccg
     4681 gcaacctgac gcataacctg atgcacttcg ggctgctgcg cggcgccagc catgcgccgg
     4741 cgtatgtgac cgattttcag ggctgggtgc gcgacaaact ggccgccggc gatgcggcgt
     4801 cgctgctgga ttaccgcgca ctcgcccccg gcgcggaagt ggcccatccc accgacgaac
     4861 acctgctgcc gctgtatgtg gcactgggcg cggcgggtgc ggattttgcc gcggaacagg
     4921 tatacagtgg catcgaacac aacatgctgg cgatggatgc ctacacattc cgctccggcg
     4981 cggattttca aacccactca cattgaagga gcgcgtcatg tcagaacccg tcatcgccca
     5041 gaaatcccct tacgccgtcg atgtggaagc cggtaaaagc tactggtggt gctcctgcgg
     5101 caaaagcaag agccagccgt tctgcgacgg ttcgcacaag gggtcggaat tcggcccggt
     5161 ggaatacaag gccgaaaaat ccggtaccgt ttatttttgc ggctgcaagc acagcgccaa
     5221 cggcgtgttg tgcgacggca gtcattccag gctgtaacgg agcaacatga tccagctacg
     5281 caaatccaac gcgcgcggcc atgccgacca tggctggctg aacgcccggc acacattctc
     5341 gttcgccgat tactacgacc ctgcctttat gggctactcg gtgctgcgcg tgattaacga
     5401 cgacgagatc gcacccgggc gcggattcgg cacccacccg caccgcgaca tggaaatcat
     5461 cacctatatc ctggacggcg ccctgcagca tcgggacagt atgggcaacg gttcggtgat
     5521 ccgcgccggc gacgtacagt acatgagcgc aggcagcggc gtgcggcaca gcgaggtcaa
     5581 cccgtccccg gatgcagcgg tgcatctgct gcagatctgg atactacctg cgcagcctgg
     5641 tctcgtaccg caatacgacc agaaaaccat cgcggcggaa cagaagcgcg gccgttggcg
     5701 catgctggct tctcccgatg gccgcgaaga ttcgatcacg atccggcagg acgcgatgct
     5761 gctggccacc atgctggaca gcgatgccga cctgacgtac agatcccagc cggggcggcg
     5821 tcaatatctg cacctggcgc gcggaacggc cgccctgaac aacgtcgcca tgcgcggcgg
     5881 cgatggcgca tttattgaaa acgaaaacct gctgacgctg tcggacgcga agggcgccga
     5941 agcgctgctt tttgatttac cttgaaaact ggagattttt atgaagaaaa ccctgatcgc
     6001 actggcactt gcagcatgga taccggcagc cctggccgat gccgaaacct ataacatcga
     6061 caacagccac agctttgcca atttcagtat acgccacgtg gtgtcgcgct tatccggcac
     6121 ttttcccgac gtgaccggga aaatcgtcat cgaccgtgcc gatctttcca aatccagcgt
     6181 ggatgccagg atcaatgtgc tgtccatcaa cacttcgctg gccaagcgcg atgagcacct
     6241 gcgcgacaag ccggaatatc tggaagccgg caagttcggc cagatgactt tcgtcagcac
     6301 caaggtcgag gccaggggca aggacgaggg cgtgctcacc ggaaaattca ctttgcacgg
     6361 cgtgaccaag gagatttcct tcccgttcaa ggtgcttggt ttcggcgccg acccctgggg
     6421 cggcgagcgc agcggcttcg aagcccatac caaactcaat gcggccgatt acggtttcgg
     6481 ctgggccgcc aatccaaacg gtccggtcgg caccgagatc gatgtcaccc tgttgatcga
     6541 aggcgtcaag gagaaagcta aatgagtctg gatttattca gcccggtcaa actgggccgc
     6601 accgagttga agaaccgcat cgtcatggcg ccgctgaccc gtaacagggc cgcgatgccc
     6661 ggcaacctgc cgcaggacat caacgtgacc tactacgcac agcgcgcctc cgccggtctg
     6721 atcgtcaccg aggcgacgcc gatctccgcc atggcccacg gctatcctgc tctgccgggt
     6781 atctacagct ccgagcaggt ggccggctgg aagaaagtgg tcgatgcggt gcatgccaga
     6841 ggcggcaaga tcttcatgca gctgtggcat gtcggccgca tttcgcaccc ctcgctgctg
     6901 ccggataacg cgctgccggt agcgccttcc gccatcaagc cggcagggca ggcctttacc
     6961 tacgccggtc tgcaggacta cgtcacgccg cgcgcgctag caacggatga aatcccgacg
     7021 ctgctcgaca agtaccgcac ggcggcgcaa aacgcgctgc aagccggctt cgacggcgtc
     7081 gaggtgcatg ccgccaacgg ctacctgatc gaccagtttc tgcgcgacgg caccaaccag
     7141 cgcaccgacc agtatggcgg gtcgatcgcg aaccggacgc gcctgctgca ggaagtggtg
     7201 gcgacggtga gtaaggtgtg gggctctgat cgcgtcggcg tgaggctgtc gccgatcaac
     7261 ccgttcaacg atatttgcga ctcctatccg caggcgctgt tcaaccacgt cgcggaagcg
     7321 ctcaacccgt tcaacctcgc ctacctgcac gtggtcgaga tcggcatggg cggcgacacg
     7381 ccgcagccgt tcgatttcgc cgaactgcgc cgggcatacc acggcaacta tatggccaat
     7441 ggcgcttacg acaaggcgcg cggcaacgct gccatcgcct ccgggcatgc cgactgcgtg
     7501 gcctatggcg tgccgttcat cgccaaccct gatctggtgg aacgctttgc caaggatgcc
     7561 ccgctcaata ttgccgacca gaacaccttt tacggtggcg gcgagcaggg ctatacggac
     7621 tacccggcgc tggcctgagt gccatgagcg gccccgtctc ctaccaactt gccatcatgc
     7681 tcgatcaacc agccagattg acggtcggcc ggctgggcgt gttcgatttt ccggcaggcc
     7741 gctatgtcta taccggcagt gccaggcgca gcctggaggc gcgcattgcg cgccacttgc
     7801 tggagcggga tgccgcccag gtcgtgcgtg tcgagtccga tcacgatgga attctcgccg
     7861 acgtcgacat cccaaaagac ttggagagat tcaccataaa ctttcaggag caaccatgaa
     7921 aaaacccgct atcacccaag tgccgatcca ggatgtcatt gccaaccgct ggagcggccg
     7981 tgcctacgac ccagccaaac cggtcagctg cgatcaggtg attgccctgc tggaagcggc
     8041 gcgctgggcg ccctcttgct atggagacca gccttggcgt ttcgtggtgt ggaacaaggg
     8101 cgaggatgcc gctgcctggc aggcggcgtt cgagacgctt gtcccgggca atcaggcatg
     8161 ggtgaaggat gcgcccttgc tgctgctggt gtgcgccgac agtctgttca accacaatgg
     8221 ccaacccaat cgctgggcgc agtacgatac cggcgcggcg gcggagaacc tgtgtctgca
     8281 agccgagacg ctgggcctga tggcgcacca gatggggggc ttcgaccccg acagggcgcg
     8341 cgccctggtt ggtgtgccag aacagtacag cctgatggct atggtatcgg taggctaccc
     8401 ggctgatccg gcaacactcg gcgcggaagt tctggcgcgt gaaacctcgc cgcgcaaacg
     8461 ccgcgaactg ggggaactgt tcttcgaggc tgtctggaac aagccggtag cctaatcccc
     8521 tggccactgc taaggggtgg gagggacgac cggggcaggc tctgcgttag cggcgcctgc
     8581 cttgttgttg atgtgcaatt cctcctgtat caggcgcacg tcggcgacga aatattcggt
     8641 atcgccgaaa caggaggtgg gcacgcccag atgctgctcg taagtgagcg acactttctt
     8701 gcccagcgtc tggttgattt tagctgccac cgcatcgtca cgtatggtga accggaattt
     8761 ttcggtcaag gtgccgggca tggtgaccat ggccagttcg ccttcccaag tcttgcacag
     8821 ccagcctttc ttggagaatt gctgcacgta gccggcgcgt tcgccggaag aatacgacca
     8881 gttcaagacc agccaggtat agagcgtaaa caggacgaga ggcgcaattg ccagcgggat
     8941 cagccagcgc gcccagcgcc gctgtttggt tggttccatg atgatgttct ccgagttggg
     9001 gcggctattt tacccgaatg gtttaagcgc cgcttctgct aaaatgccgg ctcaaccaac
     9061 gcaaaggact ataccatggc cgtcatcgaa ctcaccaaag ccaatttcaa gcagaccatc
     9121 gagaacaatg cgcttgtcat cgttgatttc tgggcgccgt ggtgcgatcc ctgcctgacc
     9181 tttaccgata ccttcaggca ggttgcggac catcacccgg atatcgtgtt cggcatggtg
     9241 aacaccgaaa ccgacaacga aatcgccgaa tattttaacg tggaacaaat tcccggcatc
     9301 ctggtggtgc gcgaacaggc cggtctgctg gcacaggtcg gtgaacttgg cgcgccggcg
     9361 ctggaagaaa tcatcaaatg ggcgcgtgac tacgacatga gcaccgtgca cgagtactac
     9421 gctggccagc agaaacaggc gtaagtgcta tcagcccgga tgtttcataa attgccgcgt
     9481 gtcctcgctg gtcttttgtt tcgtatggaa attctgttct gtcgggcgtg cgaataacta
     9541 cgccgctctt tcacaaaatc cccttgcaaa acaaattcct gcgcgatcat cacgcgaatt
     9601 tacgaaacat ccgggctagc cggtaaagct gccggtgctg accagcttga acagaaagaa
     9661 ggcgccgact cccatcattg cgaacagcat caggttggcg accttttcct gcgtatcctt
     9721 gaagttgttc gcataccatt tatccacgat caggtaggtg atcgcaagca gtggaatggc
     9781 ggctatcgca gtgaaagacc aggtttcgaa gacatggccg acggcttggc tgagccagcc
     9841 atcgggtctg aaaagatgga cggaaatgcc gactatgccg agcaacagca ggatcaactg
     9901 gccaagtgca accagcaggg atgtcaggga atccggactg cgattgttca tgatgaggcc
     9961 tccatagaag tgggaacatg gtcaatctta accctcaggc aggtattttg tcgggtccac
    10021 cggtttgccc tgcttgcgga tctcgaaatg cagtttgacg cggtcggtgt cggtgctgcc
    10081 catttcggcg attttctggc ccttggcgac ctcctggcct tccttcacca gaatctggct
    10141 gttatgtgcg taggccgaca ggtaagtctt gttatgcttg atgatgacca gtttgccata
    10201 gccgcgcaag cctgaaccgc tgtataccac ttttccgcca gcagcagcgt acactggctg
    10261 gccttgcgtg ccggcgatgt ccacgccctt tgcgctggcc gcgtcgttga acccggccag
    10321 gacttttccc ttggccggcc acgcccagtt ggccacttca ccctcttcaa tcacagggct
    10381 gactgtttca gctggcgtct cgggcttggt cgcttccggt tttaccggtt cggccggttt
    10441 ctcgctggca gcgaccgtgg cggccggctt gggaggggcg gccgccatcg ctttttcgct
    10501 gtagggttct ttgagcgcct tgggctcggt gaggagtggc ggctcgcccg ctggcttggc
    10561 ggtagccgtc gagtcggtcg cgggcttggc agcgggcact tcatccgtca gcggtttgac
    10621 ggcaggtagc ggttcggtct tcagcggcgt tgccacggtg tcctgtgacg cctggtcgtc
    10681 cgtcgctttc gtcgattcct tcagtttgag tttctggccg atacggataa cgtaaggagg
    10741 ctggatgtcg ttccactgcg cgatctcctt gtagtcgaag ccgaactcca ggccgacgct
    10801 gtacagggta tcgccttttt tgacggtgta ggtttccggg cgccagtcct tttccggcgc
    10861 tgccgatttc gatgctggcg cttcagcggc cgctggtttc gctggtgcag gtttcctggc
    10921 ttcgggagtg cgttgaatca ctggcgcctt ctggctggag gcgcagccgg ccagcagtac
    10981 cgccacgatg aacggcagca ggtgcttcat gcggtgcccc ccagcagtgg cacgaacttg
    11041 accgcctcca gcttggtctt cctgtattcc tctggggtgc gttcgatcag caccagggtt
    11101 tgctccgtgg ccccgaccgg gatcaccatg cggccgccca ccgccagctg gtgcagcaat
    11161 tcttccggga tgtgcgtggc gccggcggtg accatgatgg catcgaacgg ggcgacttcc
    11221 gccaacccca tggcgccgtc ggcgtgcttg agcttgacgt tgcgtgccct taactctcgc
    11281 aaatgcccac gcgccttcat cagcagcggg ctgatgcgtt ccaccgagta tgtctcctgc
    11341 gataccgtcg ccaacactgc cgtctggtag ccgcagccgg tgccgatttc cagtaccttg
    11401 ccgagttggt tgccgccgcg caggatttcc gtcatgcgcg cgacgatata gggctgggaa
    11461 atggtctggc cgtagccgat cggcagcgaa acgtcttcat aggcgcgaat ggacagcgcc
    11521 tcgtcgacga aaatatggcg cggcacggtg ttcatcgccg ccagtactac ctcgtcgcgg
    11581 atgccctgtt cgcgcagccg tgccaccatg cgttcgcgcg tgcgctgcga ggtcatgccg
    11641 atgccggagc ggttctggtt catgagagcc atgacttgag cgcgtccagt ttcccgtagc
    11701 gggtgagatc gacctgtagc ggcgtgatcg ccaccagccc ctgttctacc gcatgaaaat
    11761 cggtaccttc gccggcatcc tgtgccgccc cagccgcacc tacccagtac acggtttcgc
    11821 cgcgcggggt ggaggacttg accacggctt ccgccttgtg gcgcttgccc aggcgggtga
    11881 tgcgcatgcc actgacctgc tcgtagggga tgtccggcac gttgacgttg agcagcaccg
    11941 gcggcgggaa agcgtttttc tcgaagcgcc tgaccagatc cacgaccacg cgcgcagcgg
    12001 tgtcgaaatg ctgcgcacca tgctgcgaca tggataccgc aatcgagggg atgtccagca
    12061 aatagccttc catggcggcg gccacggtac cggagtagat ggtgtcgtcg cccatgttgg
    12121 caccatcgtt gatgcctgaa atcaccatgt ccggcagctg atcgagcagg ccggtgacgg
    12181 ccagatgcac gcaatcggtg ggtgtgccgt tgacgaagta gaagccgttg gcggcctggc
    12241 gcacggtaag cgggcgatcc agggtgagcg agttgctggc gccgctgcgg ttgcgttccg
    12301 tcgccacgac gacgatctct gccagcggct tgagcgcttc ggccaggatg ctgaggccga
    12361 gggcaaagta gccgtcgtcg ttggagagga gaatgcgcat ggattcgaat tataaattat
    12421 cgccgcgtca tgcgggcgta gaagatggcg ctgacgaaga atgtcaggga tagcactatc
    12481 tcgatcagcg cgagccggct ggacagcccg ttatccgacc atgcccgcat gatgccttcg
    12541 gtgaaataca gcaggatgaa catgcaggcc cactggtagg tatagcgccg cccgcgcagg
    12601 atgccgaaca atggcagcag cagtggcacg gctttcagga tcagcagcga gccgccaggc
    12661 cgcagcggcg ccagccagcc ttcccacgcc aggcacagca ggatcaatgc gaccaggctg
    12721 acgctggtgg cgagttgcaa tgttctgatc atgattgatg ggtgggagcg ccgccccggc
    12781 gcgattctgc cttcatcatc gcggcgaggg cgccgctcct acaaccgctt tgctcatcgc
    12841 gaccaaggcg gcccgcgccg attgggcgtc ggtgaccggc gcgtcgaacg tgaagcgcag
    12901 cagtttctca cccgcacgca ggtcgaagcc atcggtgtcg atgccgacca tctcggcgtt
    12961 ttccgcgtcg atccggtgta cgtgccggca ataggcgcgc aggctgtcgg cgtgatcggt
    13021 gttcatatgg gcgaggatgc cggcctcctg ttccgccagc atgttcgctg gcgggcgcag
    13081 ttcctcgccg gaaatccagt ggatgttgcc gaagccgccg atatagcgcg cctggcgaat
    13141 ttcgatgcgg tggaacagga aatcgtgcat gtcgaaatac tgtgccgcct gcggaaagta
    13201 agccagatag cgtgcccgca gcgtgccatc ctgcttgtcg gcgatggtag cctcgcccag
    13261 cagggtcagc cgggccccgg cctgcatgtc tgttgcggcc ggatcgaagg caatcagcga
    13321 cacgtgcgga ttcttctgga tgtttttggt atgttcggcg atggtggaga tcaggatcag
    13381 cgggttgccg tcgtgatcca acacgaacgg cgcgatggag ccgaacgggt agccagagaa
    13441 gcgtgcggaa tgggtggaga gcaccgcgct gcgcgtggcg cgcaggaatt tgcgggcttc
    13501 gacggccgca cctgcgctca tgtcagcttc aacgccattt tggccagccg cttgccgagt
    13561 gcgatgcaca gggttttctc ggcttccgat atcggttggt catctgtcgc cccggccacg
    13621 tggctggcgc catagggcgt gccaccaccg gtggtggagg tcagctctgg ttcggagtag
    13681 ggcaggccga cgatgaccat gccgtggtgc agcagcggga tcatcatcga tagcagtgtc
    13741 gcttcgttac cgccatgcat ggaggcagtg gaggtaaaca ctgccgctgg cttgccagcc
    13801 agcgcgcctt tcagccacag gctggaagta ccgtcgagga aatacttgag cggcgccgcc
    13861 atgttgccga agcgcgtcgg gctgcccagc gccagaccgg cgcattgctc cagatcttgc
    13921 agctcgacat acggcgcacc gttggcggga atgtctggct cggttgcctc gcacaccgtg
    13981 gaaaccctgg gtacggtacg gatgcgcgcc ttggcgccat cgaccatttc caccccgcgt
    14041 gccaccagtt gtgccatctc gcgcacagat ccgctatgac tgtagtagag gacgaggacg
    14101 tcggccatca gatgcgcttg gccagttccg ccgccttgcc gacatagcta gccggggtca
    14161 ttgccagcag ctggttcctg gcttcctgcg ggatggccag tgtggcgatg aattcgtgca
    14221 gggcttcttt ggtgatgcca cccttgccgc gtgtgagtgc cttgagttgc tcgtaggggt
    14281 tctccacgcc atagcggcgc atcacggtct ggatcggctc ggccagcact tcccagttgg
    14341 catccagatc ggcagccatg gcggcgtcat tggcttccag cttggagata ccc
//
LOCUS       DAOMFA010000023        13914 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_384_length_13914_cov_5.578469, whole genome shotgun sequence.
ACCESSION   DAOMFA010000023 DAOMFA010000000
VERSION     DAOMFA010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 13914)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13914)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13914
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_384_length_13914_cov_5.578469"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..763)
                     /locus_tag="PKW44_02245"
     CDS             complement(<1..763)
                     /locus_tag="PKW44_02245"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HQR50441.1"
                     /translation="MTSVTYSVIRPKQGVAILLLAVLAACSKSPQEGAGGMANMPPPE
                     VTVREVQPASVPVSYEYVGQTVGSREVEIRARVTGHVEKRLYREGDLVKAGQPLFQLD
                     ARPFALAVTTAKTAVAAAEADIARAEANAAQSDREQKRMTTLADASAVSRKEADDAGS
                     SAQITGAEVQVAQARLAQARAQLKEAQLNLEYASIAAPITGIVGRALKQEGALVNTGG
                     DSLLTTLVQLDPLYVTFNVADNERGRMDQELADGSL"
     gene            906..1535
                     /locus_tag="PKW44_02250"
     CDS             906..1535
                     /locus_tag="PKW44_02250"
                     /inference="COORDINATES: protein motif:HMM:NF019960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HQR50442.1"
                     /translation="MARKTKEEALETRNRILDAAEIVFQRNGVSRTTLADIASEAHAT
                     RGAIYWHFENKVDLYDAMIQRIVEPLEQKLEELQRHQDHDPLNFIRSLALFYLDSLAN
                     DPHYYRVLEIAWHKCEYVGDMARIRDQHLECGNRFLGIKESAIRSAIVRGHLPGHIDA
                     RQAAVGLMAIIDGLVAGWTLDRQRFPLSSMAAGVIDTYLAGLAAQAKNN"
     gene            complement(1545..2441)
                     /gene="xerD"
                     /locus_tag="PKW44_02255"
     CDS             complement(1545..2441)
                     /gene="xerD"
                     /locus_tag="PKW44_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HQR50443.1"
                     /translation="MSEANQELLDEFTDRLWLEEGLSRNTLESYRRDLAQFAAWLEKS
                     AGKTLLETERANVERYLAVRFPQVKPRSVSRLVSSLRRLYRFLLRESRITADPTLQIS
                     SPKLPRSLPKSLNEEEVENLLNAPDVEQTLGLRDRAMLETLYATGLRVSELVNLKVSE
                     VSLDMGVVRVMGKGSKERLVPLGEEALDWIRRYLGQGRSLILEGKLSDSLFVTQRGGA
                     MTRQAFWYLIKRYARLAGIAKPLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDISTT
                     QIYTHVARERLKQLHAQHHPRG"
     gene            complement(2413..2895)
                     /locus_tag="PKW44_02260"
     CDS             complement(2413..2895)
                     /locus_tag="PKW44_02260"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020182984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HQR50444.1"
                     /translation="MRKDYQAVIAAPFGTLGILSEAGLLFGIDFLPASLPLQAPVDPA
                     TLDTCGKLQAYLRDASAPLDLSLHLAGTEFQRRVWDAIRAIPPGCTLTYSELAQRVGS
                     GARAVANACGANPIPLVIPCHRVVAKHGLGGFMQGREAGSLSIKQWLLQHERSESGTA
                     "
     gene            3068..3481
                     /gene="rnk"
                     /locus_tag="PKW44_02265"
     CDS             3068..3481
                     /gene="rnk"
                     /locus_tag="PKW44_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HQR50445.1"
                     /translation="MASLPNITVSTLDLARLIHLLDTLPGNLQSSLDSLRMELERAKV
                     VAPEAMPPTVVTMNSTIRFAIEPTGKEFELTLSYPEDVQGQPGRISILAPVGSALLGL
                     AVGDQMEWPVPGSRTVRVRVLEVSYQPERAGEYTR"
     gene            complement(3505..4620)
                     /locus_tag="PKW44_02270"
     CDS             complement(3505..4620)
                     /locus_tag="PKW44_02270"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HQR50446.1"
                     /translation="MISLPGDPMYSNPASVCDAHGFDEACLQETMHALGLLPGNRSLV
                     KRMRAEFIVDREEDLTDAGLTALAGLMEITLLAQQPVMDLRQGWIERLRRFGEGFGTV
                     QYVADRLQWAAASAHAGIPLAFLHLQFHVTQSILVEHLLARYGHDPADLSALLPCLLK
                     LTALDLHLTITGYRPQQIEDLRKSLEELQEKAASLYQKAATDQLTGVMNFSSLMEALD
                     RQVSKAVQAGKPLCVIMADLDFFKCVNDKYGHLIGDIVLTHAAQRIKSAVRDFDLVGR
                     FGGEEFTVTLTNADLELASIIAERIRQEVAGAPFHAREHVIETTISLGVAMLQPGEMP
                     EALLERADAAMYEAKRTGRNRVVAAEDIEDAAGSAAQ"
     gene            complement(4673..5533)
                     /locus_tag="PKW44_02275"
     CDS             complement(4673..5533)
                     /locus_tag="PKW44_02275"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HQR50447.1"
                     /translation="MKRILVATDFSQQASRALRRAIALAAQHQARLDVMHVITPSSVD
                     ALCEMLPDSRILLEENLSNSATAQLYQVLDEVAPKQHLSIQPHVRISSIVDGIIEFAD
                     ANDVDLIVLGSHGEHFAHELYLGTTAENVLTKCPRSVLVVRAAPDAPYGKVGVPVDFT
                     PLSIAAVHQACELAADARITLLHAFQVPHHDRLRAMGVSEGEIKNARARFYDHASSAM
                     DRLVAGISNERISSVLEMGYPPEMIRRWVEKFDFDLIVMGSHGNRLSSVMRYALHHST
                     GDVMIFRQLA"
     gene            complement(5701..6084)
                     /gene="rplS"
                     /locus_tag="PKW44_02280"
     CDS             complement(5701..6084)
                     /gene="rplS"
                     /locus_tag="PKW44_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HQR50448.1"
                     /translation="MADIIKQLEQEEIARLGKNIPDFAPGDTVVVGVNVVEGTRKRVQ
                     AYEGVVIAKRNRGLNSSFIVRKISSGEGVERTFQLYSPLIASIEVKRRGDVRRAKLYY
                     LRERSGKSARIKEKLQMREKEVAAE"
     gene            complement(6099..6872)
                     /gene="trmD"
                     /locus_tag="PKW44_02285"
     CDS             complement(6099..6872)
                     /gene="trmD"
                     /locus_tag="PKW44_02285"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020182986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HQR50449.1"
                     /translation="MSQTLQFDVVTLFPPMFEAISKYGITSRALEKKIYELTLWNPRD
                     FTTDNYKTVDDRPYGGGPGMVMLAEPLEKAIAAAKKRQKEAAGVEHPKVIHLSPQGKP
                     LTHDAVLKLAAEPGLILLASRYEGVDERLLQRVVDEEYSIGDYVLSGGELPAMVLIDA
                     VVRHLPGTLGDADSAAEDSFVDGLLDCPHYTRPEVYEGMQVPEVLLSGNHKEIKRWRL
                     KQSLGRTRDKRPDLLAARLLTKEESRLLAEYEQEQDPHN"
     gene            complement(6869..7357)
                     /gene="rimM"
                     /locus_tag="PKW44_02290"
     CDS             complement(6869..7357)
                     /gene="rimM"
                     /locus_tag="PKW44_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020182987.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HQR50450.1"
                     /translation="MVVMGRIAAPYGVLGWVNVLPDTEMLDGLFDYPVWWVQADGGWR
                     EFPVEEAKVHGDHLVAKLRGIADRDLALKLKGKAVAVPREQLPEPEEGEYYWSDLVGL
                     AVRNVQGVELGLVKELFETGANDVLVVEGERERLIPYIDQVVLEVDVAGKRMVVDWDA
                     EF"
     gene            complement(7387..7635)
                     /gene="rpsP"
                     /locus_tag="PKW44_02295"
     CDS             complement(7387..7635)
                     /gene="rpsP"
                     /locus_tag="PKW44_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HQR50451.1"
                     /translation="MVIIRLARGGAKKSPFYSVVVADSRNRRDGRFIERVGFYNPLSQ
                     GQDSLRLNSERIAYWRSNGAQLSDRVALLVKQAAKAAA"
     gene            complement(7690..8472)
                     /locus_tag="PKW44_02300"
     CDS             complement(7690..8472)
                     /locus_tag="PKW44_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HQR50452.1"
                     /translation="MNSEALNLSILGPAFLAGLLVLATHVPLGRQVLKRGIVFIDLAV
                     AQIAALGVIAADFMGWEPQGWAVQVAAMSAALFGALLLNWMERRWPETQEALIGAVFV
                     LAASAGILLLAGNPHGGEHLKDLLAGQILWVGMSQLLPAAALTALILGLWFGFGERLG
                     HVGFYLLFALAVTASVQLVGIYLVFSSLILPALAVRNWPSRSQLAGGYAIGIAAYIAG
                     LALSAAFDLPSGAVIVWSLALVGIFANLARPVFLAIFSLPRP"
     gene            complement(8469..9371)
                     /locus_tag="PKW44_02305"
     CDS             complement(8469..9371)
                     /locus_tag="PKW44_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HQR50453.1"
                     /translation="MKKRIFTLLAMLSLTALPAHAALNVFACEPEWGALAQELGGDKV
                     SVYNATQALQDVHHVQAKPSLIAKARSANLVACTGMELEIGWLPMVLRQAGNPAVQPG
                     QPGYFEAGNYVRHLEVPQRLDRADGDIHPLGNPHIQTDPRNIGKVADVLAQRLAELDP
                     ANASFYQARQQDFSQRWQEAIKRWETQAAPLKGVALVAQHNGFPYMNEWLGLRQVATL
                     EPKPGVEPSSSHLAEVLAQLQRQPAKMVVRAAYNDDRASVWLSERAKIPAVVLPFTVG
                     GDDRAKDLFGLFDDTVQRLLEGNK"
     gene            complement(9387..10751)
                     /locus_tag="PKW44_02310"
     CDS             complement(9387..10751)
                     /locus_tag="PKW44_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR50454.1"
                     /translation="MYRPKLATCAVAAACTFPALASANDNAEMERMRAEIQQMKQSYE
                     SRIQSLENKLEQVQASAGKAEASAAKAETAAVQAGSQSTASASAFNPGISLILSGTYA
                     NLSQNPDNYTITGFQSGGEIGPGERGFSLGESELDVYGNIDHYFYGSMNLALASDDTA
                     SVEEAYIQTTALPAGFTLKAGRYFSGIGYLNAQHAHTWDFVDSPLVYQAFLGGQYGDD
                     GVQLKWVAPTDLFVEVGAEAGRGRIADTDGENKNGTAAGSVFAHLGGDVGLSNNWRAG
                     LSYLHISPNGVESNSLDNAGDYVTNTFDGTYRIWIADAVWKWAPNGNSNYTNFKLQGE
                     YMRRDASGTLFYNGSDPGHYSADQSGWYLQGVYQFMPQWRTALRYDRLDSGSVNYGAN
                     SAFLSDSSYNPDRVSLMFDYNPSEFSRIRLQLERDKSQEHETDNQIYLQYIMSLGSHG
                     AHQF"
     gene            complement(10822..11133)
                     /locus_tag="PKW44_02315"
     CDS             complement(10822..11133)
                     /locus_tag="PKW44_02315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR50455.1"
                     /translation="MMRRIFLVFTLALLFGLGQQGAAVHAVSHLADWQQSQQQPDKGQ
                     HKSTCDKCVAYAQLGGAVGSQHVLLLAPEHSYHRHTPRAVHAGFSSSHPYSARAPPSL
                     A"
     gene            11216..13327
                     /gene="ovoA"
                     /locus_tag="PKW44_02320"
     CDS             11216..13327
                     /gene="ovoA"
                     /locus_tag="PKW44_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-histidylcysteine sulfoxide synthase"
                     /protein_id="HQR50456.1"
                     /translation="MSSPLFNRTPNLADGDPEWKRQEIRAYFHATFDRYESLFETLAS
                     DKAWYRKPISLRHPLIFYFGHTATFFINKLVLAGLVQQRIDPHLESMFAIGVDEMRWD
                     DLNTAHYDWPNVEEVRVYRDQVRAVIDTVIASTPLTLPIAWEHPWWPILMGIEHERIH
                     LETSSVLIRQHELHYVQPHPAWQANRDSGAAPDNQLVAIAVGRVQLGKQRDDPYYGWD
                     NEYGSHEAEVPAFQAGKYLVSNREFLAFVEAGGYGDDALWEEEGRAWKDYTEARHPTF
                     WLRHDSKWKLRLMTEEIAMPWDWPVETNFHEAKAFCNWKMRTTGQPYRLPSEDEWFRL
                     YDFAGLSELSPDCPANANLHLDHGASSCPVTRFQQGELYDVVGNVWQWTETPIYPFEG
                     FEVHPLYDDFTTPTYDGRHNLMKGGSWISCGNEALKSARYAFRRHFFQHAGFRYVVAD
                     APAVAVSSHYETDKLLSEYAEFHYGDQYYGVPNFPQALAQLAIQAMGDKAARSALDLG
                     CATGRATFELARHFDHVTGIDFSARFIQLGVQLAEQGLLRYTLADEGELVFYKERKLA
                     DLGLEDTRHKVEFFQGDACNLKPHFSGYDLILAANLIDRLYSPRKFLATVHKRLNPGG
                     LLLIASPYTWLEEHTKREEWLGGYKRDGENVTTLDGLKETLGAHFRLLRGPLEVPFVI
                     RETRRKFQHTQSEVTIWERIV"
     gene            13324..>13914
                     /locus_tag="PKW44_02325"
     CDS             13324..>13914
                     /locus_tag="PKW44_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HQR50457.1"
                     /translation="MSFDFDQGIPRAGSGALKYDARQAIFGRAEVQPMWVADMDFATP
                     EAVTRALQARAAHPVYGYTQCPESLYESVTEWLAKRHGWQVERDCIMLCPGVVPSLNA
                     TVLALTQPGDAVIVQPPVYFPFFSAATATGRRLMENPLKLENGRYRMDFDHLEQCARD
                     GAKLLLLCSPHNPVGRVWRAEELDTLAEIAQRYGMVI"
BASE COUNT         2661 a         4406 c         4152 g         2695 t
ORIGIN      
        1 ccaggctgcc atcggccagt tcctgatcca tgcggccgcg ctcgttgtcc gccacgttaa
       61 atgtcacata caaggggtcg agttgcacca aagtagtgag caagctgtca ccacccgtgt
      121 tcaccagcgc cccctcctgt ttcaaggcgc gccccacgat accggtgatg ggcgccgcaa
      181 tgctggcgta ttcgaggttg agctgggcct ctttcagctg cgcacgtgcc tgagccaggc
      241 gcgcttgtgc cacctgcact tcggcgcctg tgatttgtgc gctagaacca gcgtcgtcgg
      301 cctccttgcg actgactgca ctggcgtcgg ccagcgtcgt catgcgtttc tgctcgcggt
      361 cggactgcgc ggcattggct tcagcacgcg caatatctgc ctccgccgcg gccacggctg
      421 tctttgccgt agtcactgcg agtgcgaacg ggcgcgcatc caactggaac aacggctgcc
      481 ccgccttcac caaatcgccc tcccggtaca agcgcttttc cacatgaccg gtgacgcgcg
      541 cgcggatttc gacctcgcgc gagccgacgg tctggccgac atactcatag ctgaccggca
      601 cgctggcagg ctgtacctcg cgtaccgtga cttcaggcgg cggcatgttg gccatgccgc
      661 ccgcgccttc ctgcggcgac ttggaacatg ccgccaacac tgccaacagc agaatggcaa
      721 caccttgttt gggccggatt acggagtagg ttacggaagt catgcagtac cccctgatgg
      781 tctgcggtct gtatgttgac agtttcgacg aatcgacgaa gcctgcataa tacatacatt
      841 caagattgta tgtaaatgaa ttcatgttaa catgattcgt aaatccattc ggttatcaca
      901 aggcaatggc tcgcaaaacc aaggaagaag cactcgaaac ccgtaaccgc attctcgatg
      961 cggcagagat tgtatttcag cgcaacggcg tttcacgcac cacgctggcc gacatcgcca
     1021 gcgaggccca cgccacacga ggcgccatct attggcattt cgagaacaag gtcgacctgt
     1081 acgacgccat gatccagcgc atcgtcgagc cgctagagca gaagctggag gaactgcagc
     1141 gccaccagga ccacgacccg ctgaatttca tccgctcgct cgccctgttt tatctcgaca
     1201 gcctggccaa cgatccgcac tactaccgcg tgctggagat cgcctggcac aagtgcgagt
     1261 acgtcggcga catggcgcgc atccgcgacc agcacctgga atgcggcaac cgcttccttg
     1321 gcatcaagga aagcgccatc cgcagcgcca tcgtgcgcgg ccatttgccg gggcacatcg
     1381 atgcgcggca ggcggcggtc ggcctgatgg ccatcatcga cggactggtt gccggctgga
     1441 cgctggatcg ccagcgcttt ccgctgtcca gcatggcggc cggggtaatc gacacctacc
     1501 tcgccggctt ggccgcgcaa gccaaaaaca actgattccc gccctcatcc gcgcggatga
     1561 tgctgcgcat gcagctgctt cagccgttct cgcgcgacat gggtataaat ctgcgtggtg
     1621 gagatgtcgg aatgccccag cagcatctgc accacccgca agtccgcgcc gtggttgagc
     1681 aaatgggtgg cgaaggcgtg gcgcagcacg tgcggcgaca gcggcttggc tatgccggcc
     1741 agcctggcat agcgcttgat gagataccag aatgcctggc gtgtcatggc cccgccgcgc
     1801 tgggtgacga acaggctgtc gctcagtttc ccttccagta tcagcgaccg cccctgcccc
     1861 agatagcgcc tgatccagtc cagcgcttct tcgcctaaag gcaccagacg ctccttgctg
     1921 cccttgccca tcacccgcac cacacccata tccagactga cctcgctgac cttgaggttg
     1981 accagctcgg acacgcgcag cccggtcgcg tacaaggttt ccagcatggc acggtcgcgc
     2041 aggcccagcg tctgctccac gtcaggcgcg ttgagcagat tttccacctc ttcctcgttg
     2101 aggctctttg gcaagctgcg cggcagcttg ggcgaagaga tttgcaatgt ggggtcagcc
     2161 gtgatgcggc tttcgcgcag caggaagcgg taaagccgcc gcaggctgga gaccagcctg
     2221 ctgacgctgc gcggcttgac ctgcgggaag cgcactgcca gatagcgctc gacgttggcg
     2281 cgctcggtct ccagcagggt cttgccggcg cttttctcca gccaggcggc gaactgcgcc
     2341 aaatcgcggc ggtaactttc cagcgtgttc cgggacagcc cttcttccag ccacagccgg
     2401 tcggtgaatt cgtcaagcag ttcctgattc gcttcgctca tgctgcagca gccactgctt
     2461 gattgacagg gaacccgctt ctcgcccttg catgaagccg cccaggccgt gtttcgcgac
     2521 gacgcggtgg catggaatca ccagcggaat ggggttggcc ccgcaggcat tggccacggc
     2581 gcgcgcgccg gagccgacgc gctgcgccag ttctgaatag gtcagcgtgc atccgggcgg
     2641 aattgcccgg atggcgtccc agacgcgtcg ctggaattcg gtgccggcga gatgcaggga
     2701 aaggtcgagc ggggcagaag catcccgcaa gtaggcctgc agcttgccgc acgtgtccag
     2761 cgttgccgga tcgaccgggg cctgcagcgg caggctggcg ggaaggaagt caatgccgaa
     2821 cagcaatccg gcctcactga gaatgcccag cgtaccgaat ggagcggcga tcacggcctg
     2881 gtagtctttg cgcatgcatc tatgttagcg caaattgccc gcgcttcaat gactgatgac
     2941 ggggtgacat ggccacagcc agaaggagca ctaaagtggc aggcatcgct gcggatgaaa
     3001 tatttgctgg atatgggcct ataatgacag atcattttgt gaaagtattt caaagagagt
     3061 tttagccgtg gcaagccttc ccaatatcac cgtttcgaca ctggatctcg cacgactcat
     3121 ccatctgcta gacaccctgc cggggaactt gcagtcaagc ttggacagtt tgcggatgga
     3181 actggagcgt gcgaaagtag tcgccccgga ggccatgccg ccgactgtcg ttaccatgaa
     3241 ctccacgatc cgcttcgcga tcgagcccac gggcaaagaa ttcgagctga ccctttccta
     3301 cccggaggat gtccaggggc aacccggaag gatttcgatt ctggcaccgg tcggtagcgc
     3361 cctgctcggg ctagcggtcg gagatcagat ggagtggcca gtacccggca gcaggacggt
     3421 acgtgtacgc gtgcttgagg tcagctacca gccggaacgg gcgggcgaat acacgaggta
     3481 gatgaaatcc ggtgcgaaag gcgttcactg cgctgcgctg cctgccgcat cctcgatatc
     3541 ctcggctgcc accacgcggt tgcgcccggt gcgcttggcc tcgtacatgg ccgcatcggc
     3601 acgctccagc agggcttccg gcatttcccc cggctgcagc atcgcaacgc cgagactgat
     3661 agtcgtctct atgacatgct cccgcgcatg gaatggagcc ccggcgactt cctgacggat
     3721 tctttcggcg atgatgctgg ccagttccag atctgcattc gtcagggtga cggtaaactc
     3781 ttcgccgcca aagcgcccaa ccagatcaaa gtctcgtaca gctgacttga tgcgctgcgc
     3841 ggcatgggtc agcacgatgt cgccaatcag gtggccgtat ttatcgttga cgcacttgaa
     3901 gaaatccagg tctgccatga tgacgcagag aggttttccc gcttgcacag cctttgatac
     3961 ctggcgatcg agagcttcca tcaggctgct gaagttcatg actccggtga gctggtcggt
     4021 ggcggctttc tggtagaggc tggccgcttt ctcctgtagc tcctctagag attttcgcag
     4081 gtcctcaatc tgctgcggcc gataacccgt gatcgtcagg tgcagatcca gtgcggtcag
     4141 cttgagcagg caaggcagca gcgccgacag atcggcgggg tcgtgcccgt agcgggccag
     4201 caggtgctcc acaaggatgc tctgggtcac atggaactgc aggtgcagaa aagcgagcgg
     4261 tatcccggca tgggcgctgg ctgctgccca ctgcaggcga tccgcaacat actgcaccgt
     4321 gccgaatccc tcgccgaatc gccgtagccg ttcgatccag ccctgcctga ggtccatcac
     4381 gggctgctgc gcgagcaggg taatttccat caggccggcc aaagcggtga gcccggcgtc
     4441 cgtcagatcc tcttcccggt cgacgatgaa ttcggcgcgc atcctcttca cgagggaacg
     4501 gttgcctggc agcagaccca aggcatgcat ggtttcctgc aagcacgcct cgtcgaatcc
     4561 atgtgcatcg cagacggatg ccgggttgga atacatggga tcgccaggta ggctgatcat
     4621 tacgccattc tcacgataag agtctggtaa caggcctgtg caggctggcg cgctatgcaa
     4681 gctggcggaa gatcatgacg tccccggtgc tgtggtgcag ggcgtacctc atcacgctgc
     4741 tgagccggtt gccatggctg cccatgacga tcaggtcgaa atcgaatttc tccacccaac
     4801 gcctgatcat ttctggggga tagcccatct ccagaacgct agaaatacgc tcgttgctga
     4861 tcccggctac cagcctgtcc attgcactgg aggcgtggtc gtagaatcgc gcccttgcgt
     4921 tcttgatctc cccctccgac acacccatcg cacgcaagcg gtcgtgatgg ggaacctgga
     4981 acgcatgcag cagggtgatt ctggcatccg ccgccagttc acaggcctgg tgcaccgcag
     5041 cgatggaaag gggcgtgaaa tccactggca ccccaacctt gccatagggc gcgtccggtg
     5101 cggcacgcac gaccagcacc gaccgcgggc acttcgtcag cacgttctcg gcggtggtgc
     5161 cgagatacag ctcgtgggcg aaatgctccc cgtggctccc cagcacgatc aggtcgacgt
     5221 cattggcatc ggcgaactcg atgattccgt cgacgatgga ggaaattctc acgtgaggct
     5281 ggatggagag gtgctgcttc ggagctactt catcgagcac ctggtagagc tgggccgtcg
     5341 ccgagttgga aagattttcc tcaagcaaga tgcgggaatc cggcagcatc tcgcacaggg
     5401 cgtccactga tgatggcgtt atgacgtgca tgacatcgag cctcgcctgg tgctgcgcgg
     5461 ccagcgcgat tgctcgccgc agcgcccgac tcgcctgctg agagaaatcc gttgcgacca
     5521 gtatacgctt catgtgcagc accccctcct catatcaggc catgtctcgt gacgtcggca
     5581 gcttagagcg gcatgtccag tatagagcgc cgtcgctata ccggcaacgc acgaggtgga
     5641 tagggaaaga aaaaacccca cgtctttcga acgtggggtt ctttgcatgg aagacagaaa
     5701 ttactctgct gccacttcct tttcgcgcat ctgcagcttt tccttgatgc gtgcggactt
     5761 gccggaacgc tcgcgcaggt agtacagctt ggcgcggcgc acgtcgccgc ggcgcttcac
     5821 ttcgatgctg gcgatcagcg gggagtaaag ctggaaagta cgctccacgc cttcaccgga
     5881 agaaatcttg cgcacgatga aggaggagtt gaggccgcgg ttgcgcttgg cgatgaccac
     5941 gccttcgtag gcctgcacgc gcttgcgggt gccttccacc acgttcacgc cgacgacgac
     6001 ggtgtcaccc ggggcgaagt cgggaatgtt cttgcccagg cgggcaattt cttcctgttc
     6061 cagttgcttg atgatatctg ccattttcac ggttcctttt agttatgagg gtcttgttcc
     6121 tgctcgtact ctgcgagcag ccgagactcc tctttcgtca acagccttgc ggccagtaaa
     6181 tccgggcgct tgtcccgggt tcgccccagc gattgtttca accgccagcg cttgatctct
     6241 ttatggttgc cggatagcaa cacttccggc acctgcattc cttcgtacac ttccggccgc
     6301 gtgtagtgcg ggcaatccag caggccgtcc acgaaggagt cctcggctgc cgaatcggcg
     6361 tcacccaatg ttccgggcag atgccgcacc accgcgtcga tcagcaccat cgccggcaat
     6421 tcgccgccgg acagcacgta gtcgccgata gaatattcct cgtccaccac acgctgcagc
     6481 aggcgctcgt ctacaccttc gtagcggctc gccagcagaa tcaggccagg ttccgccgcc
     6541 agcttcagca cggcgtcgtg cgtcagcggc ttaccctgcg gcgacaggtg gattaccttg
     6601 gggtgctcca cgccggcggc ttccttctgg cgcttttttg ccgctgcaat cgccttttcc
     6661 agcggttccg ccagcatcac catgccgggg ccgccgccgt atggacgatc atccacggtc
     6721 ttgtagttgt cggtcgtgaa atcgcgcggg ttccacaacg tcaattcata aatctttttt
     6781 tccagcgccc gcgaagtgat cccgtatttc gaaatcgcct cgaacatggg cgggaacagc
     6841 gtcaccacat caaactggag cgtctggctc aaaactccgc gtcccaatcg acaaccatgc
     6901 gtttgccggc gacatccact tccagcacca cctggtcgat gtaggggatc agccgttcgc
     6961 gctccccttc caccaccagc acgtcgttgg cgccggtttc gaacagttcc ttcaccaagc
     7021 ccagctcgac accctgcaca ttccgcactg ccagcccgac caggtcggac cagtaatact
     7081 cgccctcctc cggctccggc aactgctcgc gcggcacggc taccgccttg cccttgagct
     7141 tgagcgccag atcacggtct gcaatgcctc gcagcttggc gaccagatgg tcgccgtgca
     7201 ccttggcctc ttccaccggg aactcgcgcc atccgccatc cgcctgcacc caccacaccg
     7261 ggtagtcgaa cagcccatcc agcatctcgg tatctggcag gacattgacc caccccagca
     7321 caccgtatgg ggcggcgatc cgccccatca ccaccatgtt ttcaggcggc gattgcaaca
     7381 atggacttag gctgctgcct tggcggcttg cttgaccagc agggcgacgc ggtcggaaag
     7441 ctgcgcgccg ttgctgcgcc agtaggcaat gcgttcgctg ttcagacgca ggctgtcctg
     7501 accttggctg agcggattgt aaaaacccac gcgctcgatg aagcggccat cgcggcgatt
     7561 gcgcgaatca gctaccacca cgctgtagaa gggggacttc ttcgcgccac cgcgggcgag
     7621 acgaatgatc accataatgt tttctcttta taggtcaatt aacgaaaggg cgcgatttta
     7681 cgttattttt tatggccttg gcaaggaaaa tatggctaaa aataccggcc tggccaggtt
     7741 ggcgaatatg ccgaccagcg ccagactcca gacgatgacc gcgccggaag gcaggtcaaa
     7801 cgccgctgac agcgccagtc cggcaatata ggctgcaatg ccaatggcgt aacctcctgc
     7861 cagttggctg cgcgaaggcc agttgcgcac cgccagcgca ggcagaatga ggctggagaa
     7921 taccagatag atgcccacca gttgcaccga ggccgtcacc gccagcgcaa acagcaggta
     7981 gaaaccgacg tggccgaggc gttccccaaa cccgaaccac aggccgagga tcagcgcggt
     8041 cagcgctgcc gccggcaaca actggctcat gcccacccac aggatttgcc cggccagcag
     8101 atctttcagg tgttcgccgc catgcgggtt gccggccagc aacaggatgc cggcgctggc
     8161 cgccaggacg aataccgcgc cgatcagtgc ttcctgcgtt tccggccagc gccgctccat
     8221 ccagttcaac agcagtgcac cgaacagtgc ggcactcatg gcggcgacct gcaccgccca
     8281 gccttgcggc tcccagccca tgaaatcggc ggcgatcacg cccagcgcgg caatctgcgc
     8341 cacggcgagg tcgatgaaca cgatgccgcg cttcagtacc tgccggccga gcggaacatg
     8401 ggtggccagc accagcagcc cagccaggaa cgccgggcca aggatggaaa gattcagcgc
     8461 ctcgctattc atttgttgcc ctccagcagg cgctgcacgg tatcgtcgaa caggccgaac
     8521 aaatccttgg cgcgatcgtc accgcccaca gtgaacggca gcacaacggc ggggattttc
     8581 gcccgttccg ataaccaaac agacgcccgg tcgtcattgt aagcggcgcg gacgaccatt
     8641 ttcgccggct gtcgctgcaa ttgcgccagc acttcggcca ggtggctgct gctcggctcc
     8701 acgccgggct tgggttccag cgtcgccacc tggcgcagcc ccagccattc gttcatgtag
     8761 gggaagccgt tgtgctgcgc cacgagggca acgcccttga gcggcgcggc ctgagtttcc
     8821 cagcgcttga tcgcttcctg ccagcgttgg ctgaaatcct gctggcgcgc ctggtaaaaa
     8881 gacgcgttgg cggggtcaag ttccgccagg cgctgcgcca ggacatcagc cactttcccg
     8941 atatttctcg gatcggtctg gatatgcggg ttgcccaacg gatggatatc gccgtcggcg
     9001 cggtcgagac gctgcgggac ttccaggtgc cgcacgtaat ttcccgcctc gaagtatccc
     9061 ggctgccccg gttgcaccgc cgggttcccg gcctggcgca gcaccatcgg cagccagcca
     9121 atctccagct ccatgccggt gcaggccacc aggttggccg aacgtgcctt ggcgatcagg
     9181 ctgggcttgg cctgcacgtg gtgcacgtcc tgcagtgcct gggtggcgtt gtagaccgag
     9241 accttgtcgc cgcccagttc ctgcgccagc gcgccccatt ccggttcgca ggcgaacacg
     9301 ttgagggctg cgtgcgccgg cagcgcagtg agggagagca ttgccagcaa ggtgaagatt
     9361 cgttttttca tgattcactc cttgaattag aactgatggg cgccgtggct gcccaggctc
     9421 atgatgtact gcagataaat ctggttgtcc gtctcgtgct cctgcgactt gtcgcgctcc
     9481 agctgcaacc ggatgcggct gaactcgctg gggttgtagt cgaacatcag gctcacgcga
     9541 tctgggttgt agctgctgtc tgacaggaag gcgctgttgg caccatagtt cacactaccg
     9601 ctgtccagtc ggtcgtagcg cagtgcggtg cgccattgcg gcatgaactg gtaaacccct
     9661 tgcaggtacc agccggactg gtcggcgctg taatgtccgg ggtcgctgcc gttgtagaac
     9721 agcgtgccgg aagcgtcacg gcgcatgtat tcgccttgca gcttgaagtt ggtgtaattg
     9781 gaattgccgt tgggtgccca tttccacacg gcgtcggcga tccagatgcg gtaggtgccg
     9841 tcgaaagtgt tggtcacata atcgcccgcg ttgtccaggc tattgctttc cacgccgttt
     9901 ggcgagatgt gcagatagga cagcccggcg cgccagttgt tgctcaaacc cacatcgcca
     9961 cccaagtggg cgaacacgct gccggcggcg gtaccattct tgttctcccc atcggtatcg
    10021 gcaatgcgtc cgcgcccggc ctcggcgccg acttcgacga acaggtcggt aggcgccacc
    10081 catttgagct gcacgccgtc gtcgccgtac tggccgccga ggaaggcttg gtacaccagc
    10141 ggactgtcaa cgaaatccca ggtatgcgcg tgctgcgcgt tgaggtagcc gatgcccgag
    10201 aagtagcggc cggccttgag cgtgaaaccg gccggcagcg cagtggtctg gatgtaggcc
    10261 tcttccaccg aggccgtatc gtcggacgcc agtgccaggt tcatcgagcc gtaaaaatag
    10321 tggtcgatat tgccgtagac atccagttcc gattcaccca ggctgaagcc gcgctcgccc
    10381 ggcccgatct cgccgccgga ctggaagccg gtgatggtgt agttgtcggg attttgcgaa
    10441 aggttggcat aggtgccgga caggatcaac gaaataccgg ggttgaaggc gctggccgac
    10501 gcggtactct gactgccggc ttgaacggct gcagtttccg ccttggccgc gctggcctcg
    10561 gccttgccgg cactggcctg gacttgctcc agtttatttt ccagggactg gatgcgcgac
    10621 tcgtaacttt gcttcatttg ctggatttcg gcgcgcatgc gctccatttc cgcgttgtcg
    10681 ttggcgctgg ccaacgccgg aaaggtgcaa gccgcggcga cggcacaagt cgccagtttt
    10741 ggtctgtaca tgatatagcc tcgcgtctga acgaacacgg ccgcccgcag tggcgaacgg
    10801 caatcttaag gtgttaccgg ttcaggcgag agaaggcggg gcgcgggcag aataagggtg
    10861 gctggaggag aagccggcat gtacagcgcg cggcgtatgg cgatggtaac tgtgttccgg
    10921 ggcaagcaac agcacatgct gcgaccccac ggcgccaccc agttgggcat aggcaacgca
    10981 cttgtcgcaa gttgacttgt gctggccctt atccggctgc tgctggcttt gctgccagtc
    11041 cgcaaggtgc gaaacggcgt gcaccgccgc accctgctgg ccaagaccga acagcagggc
    11101 aagggtgaaa acgagaaaaa tgcggcgcat catgggattc attctagcat ggcaataggt
    11161 aacgatttgg gcttatagtt tccccatcca ctgacttggt aagcgctttc acacgatgag
    11221 ttccccgcta ttcaaccgca cgccaaatct ggccgatggc gaccccgaat ggaagcgcca
    11281 ggaaatccgc gcctatttcc acgccacctt cgaccgctac gagtcgctgt tcgagaccct
    11341 ggccagcgat aaagcctggt accgcaagcc gatcagcctg cgccacccgc tgatcttcta
    11401 cttcggccat accgccacct tcttcatcaa caaactggtg ctggccgggc tggtgcagca
    11461 gcgcatcgat ccgcacctcg agtccatgtt cgccatcggc gtggacgaga tgcgctggga
    11521 cgacctcaac acggcacact acgactggcc gaatgtcgaa gaggtgcgcg tctaccgtga
    11581 ccaggtacga gcggtgatag ataccgtcat cgccagcaca cccttgaccc tgccgatcgc
    11641 ctgggagcac ccctggtggc ccatcctgat gggcatcgaa cacgagcgca tccacctgga
    11701 aacgtcgtcg gtccttatcc gccagcacga gttgcattac gtccagcccc acccggcttg
    11761 gcaagccaac cgtgattcag gcgcggcgcc ggataaccag ctcgtcgcca tcgccgtcgg
    11821 tcgcgtgcag cttggcaagc agcgcgacga cccgtattac ggctgggaca acgagtacgg
    11881 ctcccacgag gccgaagtcc ctgccttcca ggctgggaag tacctggtca gcaatcgcga
    11941 gttcctcgct ttcgtcgagg ccggcggcta tggcgacgac gcgctgtggg aagaggaagg
    12001 gcgcgcctgg aaggattaca ccgaggctcg ccaccctact ttctggctac ggcacgacag
    12061 caagtggaag ctacgcctga tgacggagga aattgccatg ccttgggact ggccggtgga
    12121 aaccaatttc cacgaagcca aggccttctg caactggaaa atgcgcacca ccggacagcc
    12181 ttatcgtttg ccgtccgagg acgagtggtt ccgcttgtac gatttcgccg gattgtcgga
    12241 gctttcgcca gattgtccgg ccaacgccaa cctgcacctg gatcacggcg cttcctcctg
    12301 cccggtcacc cgctttcagc aaggcgagct gtacgacgta gtgggcaacg tctggcaatg
    12361 gacggagacc ccgatctacc cgttcgaggg cttcgaggtg catccgttgt acgacgattt
    12421 caccacgccc acctacgacg gccgccataa tctgatgaag ggcggctcct ggatttcctg
    12481 cggcaacgag gcactgaaaa gcgcgcgcta tgccttccgc cgccacttct tccagcatgc
    12541 cggcttccgc tatgtagtag ccgatgcgcc cgccgtggca gtgtcgtcgc actacgaaac
    12601 cgacaagctg ctgtccgaat acgccgagtt ccactacggc gaccagtatt acggcgtgcc
    12661 caacttcccg caggcattgg cgcaattggc gattcaggcc atgggcgaca aagccgcccg
    12721 cagcgcgctc gacctcggct gcgccaccgg acgcgccacc ttcgagctgg cccgccattt
    12781 cgaccacgtc accggcatcg acttctccgc gcgcttcatt cagcttggcg tgcaactggc
    12841 cgagcaagga ctgctacgct acactctggc cgacgagggc gaactggtgt tctacaagga
    12901 gcgcaagctg gccgacctcg ggctggaaga tacgcggcac aaggtagagt tcttccaggg
    12961 cgacgcctgc aacctcaagc cgcacttcag cggctacgac ctcattctgg ccgccaacct
    13021 gatcgaccgc ctgtacagcc cgcgcaagtt cctcgccacc gtgcacaagc gtctcaaccc
    13081 cggcggcttg ctgctcatcg cctcgcccta cacctggctg gaagaacaca ccaaacgcga
    13141 ggaatggctg ggcggctaca aacgcgatgg cgaaaacgtc accaccctgg acggcctgaa
    13201 ggaaacgctg ggcgcacatt tccgcctgct gcgcggcccg ctggaagtgc ccttcgtgat
    13261 ccgcgaaaca cgacgcaagt tccaacacac gcaatcggaa gtgaccatct gggagcgcat
    13321 cgtttgagtt ttgacttcga ccaaggcatc ccccgcgccg gcagcggcgc gctcaaatac
    13381 gatgcgcggc aggccatttt cggccgcgcc gaagtacagc cgatgtgggt ggcagacatg
    13441 gacttcgcca cgccggaggc ggtaacgcgg gcgttgcagg cgcgtgcggc gcatccggtg
    13501 tacggctaca cccagtgccc ggaaagcctg tatgaatccg tgacggaatg gctggcgaag
    13561 cgccacggct ggcaagtcga gcgcgactgc atcatgcttt gccccggcgt ggtaccgtcg
    13621 ctcaatgcca cggtgctggc gctgacgcag ccgggcgacg cagtcatcgt gcaaccgccg
    13681 gtctatttcc cgttcttttc cgccgctacc gccaccgggc ggcggctgat ggaaaaccca
    13741 ctcaagctgg aaaacggccg ctaccgcatg gatttcgatc acctggagca atgcgccaga
    13801 gacggcgcaa agttgctgct gctgtgctcg ccgcacaacc cggtggggcg ggtgtggcgg
    13861 gcggaggaac tggatacctt ggcggaaatc gcgcagcgtt acggcatggt gata
//
LOCUS       DAOMFA010000024        13723 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_392_length_13723_cov_5.314311, whole genome shotgun sequence.
ACCESSION   DAOMFA010000024 DAOMFA010000000
VERSION     DAOMFA010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 13723)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13723)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13723
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_392_length_13723_cov_5.314311"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            96..1331
                     /locus_tag="PKW44_02330"
     CDS             96..1331
                     /locus_tag="PKW44_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HQR50458.1"
                     /translation="MKLPSLNTQILLGALAGVSLGLWFAHMEPAAVMAQHGLYAAGLL
                     GNLFIDLLKMILVPLVFTSIAVGVANLRAHRQMHRVWVATLGFFVFSMAVAMVLGMGA
                     ANLFEPGKGLDLALFHDAMQGFQAKQMTLAEFGQSFLHGLFVNPFAALAQGNVLAIVI
                     FALFTGIALVAGGERYRNILALLQEGMEITLRIVGWIMRLAPLGIMALLVKLVATQDV
                     ALLATLGKFVTVVLGTTLLHGVAVLPLIAYLVAGVSPLRFWHGAREALVTAFATSSSS
                     ATLPVTLRCVEQHLHVKPEIAGFVVPLGATVNMDGTALYEAAAALFIANLAGIDLNLM
                     QQGIVFVTAMLAAIGAPGIPSAGMVTMVMVLQSVGLPAEAIAILLPIDRLLDAFRTAV
                     NVEGDMVGSLVVQKLVNRP"
     gene            complement(1318..4386)
                     /locus_tag="PKW44_02335"
     CDS             complement(1318..4386)
                     /locus_tag="PKW44_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HQR50459.1"
                     /translation="MTLPELSIKRHVLAWMLSGVLVLFGIISYQRIGVDRFPYIEFPV
                     ISVSTTEKGANPDIIDASITNVIETSVNSVPGIEHIQSSSSPGVSNVTITFSLDKRVD
                     VAFNEVQTKVNQVLRRLPKDADPPVVAKVETGASPIMWMALRGDRTQQQLNQYALNVL
                     KKRLETVNGVGEVRLGGRRDRTIRVNLLPERMAALGVTAQDLQKAFDNEHVQLPGGFM
                     VGQKSEYLFKLDMEFHKLSDLGTMIVAYRDGAAIRLKDVAEIEDGLGDFRQLAHFNGE
                     RTVGLGIVKIANANTVAIIDEVKRRLDSEIRPQLPPGMTIEIASNDALYIQEIINALL
                     EHLVAGTLLAAAIVWLFLRSIRSTLIIATAIPVSLLGAVAVMYFGGYTFNVMTLLALL
                     LLIGVVVDDAIVVLENIFRHREHLDADPVSAAINGSKEVAFAVLAATLSLVSIFAPVI
                     FMSGIIGQFFKSFAVVVTFGVLVSLFVSLTLTPMLCSRYLKVSANHGRFYYALDAFFR
                     GMDNVYRTLLRLALSHRWKVLLITLFAVLSSAWFFQQVKKEFTPEEDEGRFIVYLKTP
                     LGSSVDYTNTRLVEVEKILGKHPEIATYFTGIGIGQTGQVNQGFAYVRMQDRDKRSIR
                     QQDTLKKLREEFSQVPGVRAFPAPVPIVQGQRGDPLQFNLSGPNLGQVASLAQQLQQK
                     LSNVPGMGKVDLDMQLDLPELVMDIDRTRTADLGLSAQDVAFSLNMLTGGIDIAKYSD
                     DPGDGERYDIRIKGKDGSFGQPADLTKIYLRNRDGNMVRLDTVARFKPVLGAAVIGRF
                     DLQYAANFFSTPILPLGEAVSKVQETAKPLLPPGYAIKFTGQAEEFGKTAKNMVFAFV
                     MAMILLYMVLASQFNSFLQPFIIMLAQPLAIIGGVMALWLTGHSLNIFSMIGLVLLIG
                     LVAKNSILLVDLTNQRREQGMAIDSALQDACPIRLRPVLMTSATIVLALLPAALGFGA
                     GSETNGPLSVAVIGGMITSTLLTLVVVPAVYSLAMNGAEQLERRLAARAG"
     gene            complement(4383..5462)
                     /locus_tag="PKW44_02340"
     CDS             complement(4383..5462)
                     /locus_tag="PKW44_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HQR50460.1"
                     /translation="MNKLCLLLAAALLLPACTKKAEDKSQAAAPPATLVTVTQAQTRQ
                     LEIFEQTIGSMESLIDPGVGAEVAAKVLKIFAHAGQEVKKGQLLAILDATDYTLQKRE
                     AEAEIARIEALLANQGRIVERNQHLVQKNFISQNALEDASTQQSALREQLEAARARVA
                     SIEHNGAKTQVFSPIDGRVEKQIVSPGDFVKQGDSLFQLIGTQKLRAHLPFPENVAAK
                     LHPGQTVRLSTPTVPDQVYTTTIKEIKPLIGSNRAVDIIADVVDQHGWQGGASVNGKV
                     ILGEHTQAVVVPEASVVLRPAGEVVYVIKDKAAAQHVVKTGLRQEGLVEITEGLNAGE
                     IVAVDGAAYLTDKAPVNVQQQSGQK"
     gene            complement(5459..6106)
                     /locus_tag="PKW44_02345"
     CDS             complement(5459..6106)
                     /locus_tag="PKW44_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HQR50461.1"
                     /translation="MQAIKVQAEPADASSTERILAAAEALFSEQGFDAVSMNAIAARA
                     GVSKANIFHHFSNKNALYLAVVKDACKESQVRLQHMESSTSSLAERLTHFARSQLQSM
                     LDHGQTTRLILRELLQNGPEHGRELAEQVFGQSFARLVEMIRDGQTRSELRREIDPAA
                     VAVMLIAINVYFFEARDVLRHYPDVDFADDPQRYSEKLLQLMLHGILPAPKEPQQ"
     gene            6272..7498
                     /locus_tag="PKW44_02350"
     CDS             6272..7498
                     /locus_tag="PKW44_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018508489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HQR50462.1"
                     /translation="MELHQFFLLFAIILISARVFSEVVSRFGIPGVIGELLAGLIVGP
                     SMLGWVTPDATMKLLAEIGIILLLFEVGMDTDLNRLARSGAKPVIVATVGFVLPFLLG
                     YGVSAWGFGLPELTALFIGGTLTATSIGITVRVLDDLGRRHSDEAQIVIGAAVLDDIL
                     GVLALAFLYQFAVEKQVSVQALGEVSLYILLFMLLTPLAAKLAAGIIDHLDRRAATPG
                     LLLTMALSLILLFSWLAHLVGAPVIMGGFAAGLAFGQHFGLNLKIRLPFGRELSFTPS
                     PKLAHRLEEQMRPLIHTFAPLFFVMVGVSLNLSAVDWDSAFIWELATLLLIAAFIGKL
                     AAGYCIREPRRAQTAIGLAMIPRGEVGLIFAQLGFTQNILNGELYAAMLIVIALTTMA
                     PPFLLKWFYGSPSVTG"
     gene            complement(7461..8024)
                     /gene="ampD"
                     /locus_tag="PKW44_02355"
     CDS             complement(7461..8024)
                     /gene="ampD"
                     /locus_tag="PKW44_02355"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013146969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HQR50463.1"
                     /translation="MRMFNLDDTGTARQALQITSPNSDERPIGTVIDLVVIHNISLPP
                     GEFGGDGVVELFTNRLDPAEHPYYAEIHQFKVSSHFFVRRSGEVIQFVPCGKRAWHAG
                     VSQWQGRERCNDFSIGIELEGDDTTRFEDVQYATLEALLTAIEQRYPIAGIVGHSDIA
                     PGRKTDPGPFFDWTRINRSQKDCRRTI"
     gene            8075..9076
                     /locus_tag="PKW44_02360"
     CDS             8075..9076
                     /locus_tag="PKW44_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019896912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:calcium antiporter"
                     /protein_id="HQR50464.1"
                     /translation="MVFLELFVMLVVILVAAEVFTNALEHLGEKLKISEGVTGSLFAA
                     VGTALPETLVPLLALLAGTKNVQVNEEIGVGAILGAPLMLSTLSLSLLAFAVLRKRGP
                     HGEFRPERTGLLRDLDFFLAAFAFATLALFIPHTMTEVRAALGFTMVLIYFIYIMLTL
                     RASKALVHDGHATEAEEPMFLCRAGLPNNMLVILLQLAGGLALLVYGAHGFIHGVEAA
                     APLLGLSALLLSLLIIPIATELPEKVNSILWVRKHKDTLAFSNITGAMVFQGTLLPAI
                     GIMLTPWEPRREVLAGVVITLLAAAWLRWMASRGHLKVWMVWINGALYLTYLAVALA"
     gene            complement(9084..10454)
                     /locus_tag="PKW44_02365"
     CDS             complement(9084..10454)
                     /locus_tag="PKW44_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HQR50465.1"
                     /translation="MSNKSRNMHACLVVDDETDIRELLLLTLERMDVPAMGAGTLEEA
                     RHLLQRHDFGLCLTDMRLPDGNGLDLVRHIGQTCPGLPVAVITAYGSAENAVAALKAG
                     AFDYLSKPISLDQLRPLVQSALRLAEPHAADEGELALIGDSAPVRQVRLMIDKLARSQ
                     APVYISGESGSGKELAARLIHAKSSRGDGPFVPVNCGAIPETLMESEFFGYKKGAFTG
                     AQQDKPGVFHVANGGTLFLDEVADLPLHMQVKLLRAIQEKKVRMVGGAQEEAVDVRII
                     SATHRNLGKMMEAGQFRQDLYYRLNVIELKMPSLRDMQEDIPQLAGKILEKHSRELGA
                     AQPELSATAMTLLTQYAFPGNVRELENVLERALALCDGKRIEADDLLLELVSQDSEAA
                     ASAAMNSLPEYLESIEKKAILDALQKSGNNRTAAAKLLGVSFRALRYRLAKLGLSRED
                     DKEDEI"
     gene            complement(10444..12048)
                     /locus_tag="PKW44_02370"
     CDS             complement(10444..12048)
                     /locus_tag="PKW44_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQR50466.1"
                     /translation="MTLPEPSHPAGHPDAYWRTMRYFTLYRLVVSGILLGTYFLMSER
                     VLRENYDNTLYLQAASAYVVFSLAAIFFTRLRLPRFNRQLTGQVIGDIGFIVLLMSAA
                     GGVKSGLGLLLVIAIAAAALISQGRLALFYAALATIALLLEQSWRILALDESFESYSH
                     VVMLGLSCFATAWLAHSLAQRTRQSEELASQRGIDLENQALVNQIVIRDMLDGVLIVD
                     KDLRLRHHNAQAEAMLGGLVSDVTAPTLEGFSSEIAALFRQWLKARNDARANEVRVTA
                     GSKELRLRFQPVGADRSQGAVIFIEDWDRVQSQARQLKLASLGRLTANVAHEIRNPLS
                     AISHASQLLQEDRGQDVTHQRLLQIINDNVVRLDQIVKDVLQLNQRDRLQQEIIHLPD
                     FLSEFREQFCQVENIPPEDLALDIAEDASALFDRRHLHQVLWNLCRNGWRHGRKRAGS
                     LCLRLEQSRRKEEIVIAVKDDGDGVPDEVRPHLFEPFFTTESGGTGLGLYIARELCEA
                     NGASLGYAGSDAGGQFNIYLKKHHVQ"
     gene            complement(12045..12266)
                     /locus_tag="PKW44_02375"
     CDS             complement(12045..12266)
                     /locus_tag="PKW44_02375"
                     /inference="COORDINATES: protein motif:HMM:NF041023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP0621 family protein"
                     /protein_id="HQR50467.1"
                     /translation="MLGKLILLALAVWLLVFILGQYRRNMDRPAPKSPGAQDMVQCRV
                     CGVHLPKSESVASRGGYYCCEEHSRQSKQ"
     gene            12388..12948
                     /locus_tag="PKW44_02380"
     CDS             12388..12948
                     /locus_tag="PKW44_02380"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HQR50468.1"
                     /translation="MATPLELSDFLAQTERRAFRQAAYAVRDDHVALDIVQDAMLKLA
                     EKYTARPVTEYPMLFQRILQNTIRDHFRRQKVRNLWTTLLSSFSTGEDGDEHDPLETL
                     QTGDDGQDDTREQIERKQTLAIIEAALQRLPTRQREAFVLRYWEDLDVAETAAVMGCS
                     EGSVKTHCSRAVHALAASLKTKGISL"
     gene            12945..13319
                     /locus_tag="PKW44_02385"
     CDS             12945..13319
                     /locus_tag="PKW44_02385"
                     /inference="COORDINATES: protein motif:HMM:NF023699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3619 family protein"
                     /protein_id="HQR50469.1"
                     /translation="MTSEQKIAKEIVSLLDYGTTQVDQAASDRLYAARRQAVAVAQAR
                     ISPVASTGAGNFLLEHLHGQQAWKLPLLLLGVVLAALLLTQQITESGPVEEDALLLGS
                     DLPPEAYLDKGFDAWLEPSSQH"
     gene            13292..>13723
                     /locus_tag="PKW44_02390"
     CDS             13292..>13723
                     /locus_tag="PKW44_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3106 domain-containing protein"
                     /protein_id="HQR50470.1"
                     /translation="MARTLLAALILSSAIGVANARAEGAPVAEPHWSQLSNEQKQVLA
                     PLASQWDTLRPWQRERMLDIARDYPRMPPQQQGRVQQRLRDWSRMTPFERENARKKYQ
                     QFQLLPPEKQEEMRRKWSDYQRLPEAERQKLREKQPESTPVD"
BASE COUNT         2676 a         4131 c         4077 g         2839 t
ORIGIN      
        1 ggacagccaa accaaagaca tcagcgacta aatccaaggt tgcgccaaag ccgaaaaaat
       61 ctgccaaggt atccgataaa tccagctaac ccaccatgaa gctgccatca ctgaataccc
      121 agatcctgct gggtgcgctt gcaggggtta gcctcggcct gtggtttgcc cacatggagc
      181 cggcggctgt gatggcgcaa catggtttgt atgccgccgg actgcttggc aacctgttca
      241 tcgacctgct gaaaatgatc ctggtgccgc tggtattcac ctccatcgcc gtaggggtgg
      301 ccaacctgcg cgcgcaccgg cagatgcatc gggtgtgggt cgcgacgctg ggtttcttcg
      361 tgttctccat ggcggtcgcc atggtgctgg gcatgggcgc ggccaacctg ttcgagcctg
      421 gcaaagggct ggatttggcg ctgttccacg acgccatgca gggcttccag gccaagcaga
      481 tgacactggc cgagtttgga cagagctttt tgcacggcct gttcgtcaac cctttcgcgg
      541 cactggcaca gggcaacgtg ctggccatcg tcatcttcgc cctgttcacc ggcatcgccc
      601 tggtggcagg cggcgaacgc taccgcaaca tcctggcgct gttgcaggaa gggatggaaa
      661 tcacgctgcg cattgtcggc tggatcatgc gcctggcgcc gctgggcatc atggcgctgc
      721 tggtgaagct ggtggcgacg caggatgtag cgctgctggc cacactggga aaattcgtca
      781 cagtagtgct tggcactacc ctgctgcacg gggttgcggt actgccgttg attgcctatc
      841 tggtcgccgg cgtttcgccg ctgcgtttct ggcacggcgc gcgcgaggca ctggtgacag
      901 catttgccac cagctccagt tccgccaccc tccccgtgac gctgcgctgc gtggagcagc
      961 atctgcacgt caagccagag atcgccggct ttgtcgtgcc gctgggcgcc accgtcaaca
     1021 tggacggcac cgcactgtat gaggccgccg ccgcgctgtt catcgccaac ctggccggca
     1081 tcgacctcaa cctgatgcag cagggcatcg tcttcgtcac cgccatgctg gccgccatcg
     1141 gcgcccccgg catccccagc gccggtatgg tgaccatggt catggtgctg caatcggtgg
     1201 ggctgccggc cgaagccatc gccatcctgc tacccataga ccgcctgttg gacgccttcc
     1261 gtacggcggt caacgtcgaa ggtgacatgg taggtagcct ggtggtgcag aagctggtca
     1321 accggcccta gctgccagtc ggcgctccag ctgctctgcc ccgttcatcg ccagcgaata
     1381 cactgccggc accaccacca gcgtgagcag ggtggaggta atcatgccgc cgatcaccgc
     1441 caccgacagt ggtccgttgg tttcggaacc ggcgccgaaa cccagcgcag cggggagcaa
     1501 agcgagaacg atggtagcag aggtcatcag caccggtcgc aaccgtatcg gacatgcatc
     1561 ctgcaaggcg gagtcgatgg ccatgccttg ctcgcggcgc tggttggtga ggtcgaccag
     1621 caggatggag ttcttggcca ccagaccgat cagcagtacc agcccgatca tggagaagat
     1681 gttaagcgaa tgaccggtca gccacagcgc catcacccca ccgatgatgg cgagcggttg
     1741 cgccagcatg atgatgaagg gctgcaggaa ggagttgaac tggctggcca gcaccatata
     1801 cagcaggatc atcgccatga cgaaagcgaa caccatgttc ttggcagttt tgccaaactc
     1861 ttccgcctgg ccggtgaact tgatggcata gccgggcggt agcaagggtt tggcggtttc
     1921 ctgtactttg ctgacggcct cgcccaacgg aaggatgggg gtgctgaaga agttggcggc
     1981 atactgcaag tcgaagcgac caatgaccgc ggcacccaac accggcttga acctggccac
     2041 ggtgtccagc cgcaccatgt taccgtcacg gttacgcagg tagatcttgg tcagatcagc
     2101 cggctgacca aaactgccgt ccttgccctt gatccggatg tcgtagcgct cgccgtcgcc
     2161 cggatcgtcg ctgtacttgg caatgtcgat gcctccggtc agcatattca gcgagaatgc
     2221 cacgtcctgt gccgacaggc cgaggtcagc cgtgcgcgtg cggtcgatgt ccatcaccag
     2281 ttccggaagg tcgagctgca tgtcgaggtc gaccttgccc atgccgggaa cattggagag
     2341 tttttgctgc aactgctggg ccaggctggc gacttggccc agattgggac cagacaggtt
     2401 gaattgcaat ggatcaccgc gctgaccctg cacaatgggc accggcgccg ggaaggcacg
     2461 cacgcctgga acctgagaga actcctcgcg cagcttcttc agggtgtcct gctggcggat
     2521 gctgcgcttg tccctgtcct gcatacgtac gtaagcaaag ccctgattga cctgcccggt
     2581 ctggccgatg ccgatgccag tgaagtaggt agcaatttcc ggatgcttgc cgagaatttt
     2641 ctcgacctcc accagacggg tattggtgta atccacgctg gagccgagcg gcgtcttgag
     2701 atagacgatg aagcggcctt catcctcttc cggggtaaat tccttcttta cctgctggaa
     2761 aaaccaagcg ctggaaagaa ctgcaaacag cgtaatcagc agtactttcc agcggtggct
     2821 aagtgccagt cgcagcaacg tgcggtagac gttgtccatg ccgcgaaaga acgcatccag
     2881 cgcatagtag aagcgcccgt ggttggcgct tactttcagg tagcgcgagc acagcattgg
     2941 cgtaagggta agagaaacga atagcgacac cagcacgccg aaggtgacca ccacggcaaa
     3001 cgacttgaag aactggccga tgatgccgct catgaaaatc accggcgcga agatggagac
     3061 cagggaaagg gttgccgcca gcacggcgaa ggcaacctcc ttgctgccgt taatggcggc
     3121 gctgaccgga tcggcgtcca ggtgttcgcg gtggcggaaa atgttttcca aaaccacaat
     3181 cgcgtcgtcc accaccacgc cgatcagcaa cagcagagca agcagggtca tgacattgaa
     3241 ggtgtaacca ccgaaataca tcactgccac tgcccccaac agggacaccg gaatcgccgt
     3301 agcgataatg agggtggagc ggatggagcg caggaatagc cagacgatgg cggcagccag
     3361 tagcgtaccg gccaccagat gctccagcaa tgcgttgatg atctcctgga tatagagcgc
     3421 atcgttggaa gcgatttcga tggtcatgcc gggcggcagt tgcgggcgga tttcgctatc
     3481 caggcggcgc ttcacttcat cgatgatggc aaccgtattc gcgttggcaa tcttgacaat
     3541 gcccagaccc acggtacgct cgccattgaa gtgcgcaagc tggcggaagt cgcccagacc
     3601 atcctcgatc tccgccacgt ccttgagccg gatcgccgcg ccgtcgcgat aggccacgat
     3661 catggtgcca agatcggaaa gcttgtggaa ttccatgtcc agcttgaaca ggtattcact
     3721 cttctggcca accatgaagc cgccgggaag ttgcacgtgc tcgttatcga aggctttttg
     3781 cagatcctgc gccgtaaccc ccagcgccgc catgcgctcc ggcagcaggt tgacgcggat
     3841 ggtacggtcg cggcgaccac ccaggcgtac ttcgcccacg ccgttgacgg tttccagacg
     3901 cttcttcagc acgttgagcg cgtactggtt gagctgctgc tgggtacggt caccgcgtag
     3961 cgccatccac atgatgggcg aggcgccggt ttccaccttg gccaccaccg gcgggtcggc
     4021 atccttgggc aggcgacgca gcacctggtt gaccttggtc tgtacctcgt tgaaggcaac
     4081 gtccacccgc ttgtccaggc tgaaggtgat ggtgacgttg gatacgccgg ggctggaact
     4141 ggactggatg tgctcaatgc cgggtacgct gttgaccgaa gtctcgatca cgttggtgat
     4201 gctggcgtcg atgatgtccg ggttggcgcc tttttccgtg gtgctgacgg agatcaccgg
     4261 aaattcgata tagggaaagc ggtccacgcc gatacgctga tagctgatga tgccgaacag
     4321 caccagcacg ccggacagca tccatgccag gacgtggcgt ttaatggata gctctggcag
     4381 cgtcatttct ggccactctg ttgctggacg ttgaccggtg ccttgtcagt caggtaggca
     4441 gcaccatcca ccgcaacaat ttcaccggca ttcaggcctt cggtgatttc caccagtccc
     4501 tcctgccgca gtccggtttt gacgacgtgc tgagcggccg ctttatcctt gatgacgtag
     4561 accacctcgc ccgccgggcg tagcaccacg ctggcctcgg gcaccaccac tgcctgtgtg
     4621 tgctcgccca ggataacctt gccattgacg ctggcgccgc cctgccagcc atgttgatcc
     4681 accacgtcgg cgatgatgtc gaccgcgcga ttgctgccga tcagcggttt gatttccttg
     4741 atggtggtgg tatagacctg atctgggact gttggtgtcg agagcctcac ggtctggccc
     4801 ggatgcagtt ttgccgcgac attctccgga aacggcaggt gcgcgcgcag cttttgcgta
     4861 ccgatcagct ggaacaacga atcgccctgc ttgacgaaat cgccgggaga aacgatctgc
     4921 ttttccacgc ggccatcgat gggcgaaaat acctgagtct tggcaccatt gtgctcgatg
     4981 gaggcgaccc gcgcacgtgc cgcttccagt tgttcgcgca gggcgctttg ctgggtggag
     5041 gcatcttcca acgcattctg cgaaatgaaa tttttctgca ccaggtgctg attacgctct
     5101 acgatgcgcc cctgattggc cagcagggcc tcaatgcggg cgatttccgc ctcagcctcg
     5161 cgtttctgca gggtgtagtc ggtggcatcc aggatggcca gcaactggcc ttttttcact
     5221 tcctgcccgg catgggcgaa gatcttcagc accttggccg ccacttccgc gccgacgccg
     5281 gggtcgatca ggctttccat gctgccgatg gtctgctcga aaatttccag ctggcgggtt
     5341 tgcgcctggg tgacggtcac cagcgtggcg ggcggcgcgg cggcctggct tttgtcttcg
     5401 gcttttttgg tgcaggccgg caacagcaag gcggccgcca gtaacaggca gagcttgttc
     5461 attgctgtgg ttccttgggg gcaggcagga tgccgtggag catcaactgc aacagtttct
     5521 cgctgtagcg ttgcgggtca tcggcaaaat cgacatcggg gtaatgtcgc agcacgtcac
     5581 gcgcctcgaa gaaatagacg ttgatggcga tcagcatcac cgccaccgca gccgggtcaa
     5641 tttccctgcg cagctcacta cgcgtctgcc cgtcgcgtat catctccacc aaccgggcaa
     5701 agctctgccc gaacacctgc tccgccagct ctcgcccgtg ttcaggcccg ttttgcagca
     5761 gttcgcgcaa aatcagtcgc gtggtctggc catgatcgag catggattgc aattgtgaac
     5821 gggcaaaatg cgtaaggcgt tcggccagac tgctggtact gctctccatg tgctgtagac
     5881 gaacctggga ttccttgcag gcgtccttga ccacagccag atacaatgcg ttcttgttgc
     5941 tgaagtgatg gaagatgtta gccttgctga ccccggctct ggcagcgatg gcgttcatgg
     6001 aaacggcgtc aaaaccctgc tcggagaaca gcgcttcggc ggcggccaga atacgctccg
     6061 tgctgctggc atcggcgggt tccgcctgga ctttgatggc ttgcatggat gatatccgac
     6121 ccgtttaaag tactaagtct gccactgact gaccggttgg tcaattcctt gcatcgtggg
     6181 aaagcgctat gatggccaag ccaaagtcaa acccggtatc gccatcgact gatttctgcc
     6241 aggccgccat tcctgaatcg agaaagctta catggaactg catcaattct ttctgctttt
     6301 tgccattatt ctgatctccg cgcgcgtgtt ttccgaagtg gtttcacggt ttgggattcc
     6361 cggagtgatc ggcgaattac tggccggcct aatcgttggg ccgtcaatgc tgggctgggt
     6421 aacgccggat gccaccatga aacttctggc ggaaatcggc atcatcctgc tgttgttcga
     6481 agtcggcatg gataccgatc tcaaccggct ggcgcgctcc ggcgccaagc ccgtaatcgt
     6541 agccacggtc ggtttcgtgc tgcccttcct gctgggctac ggcgtcagcg cctgggggtt
     6601 cggcctgccc gagctgacag cgttattcat cggtggcaca ctgactgcca ccagcatcgg
     6661 catcacggta cgggtgctgg atgatcttgg gcggcggcat tcggacgagg cgcagattgt
     6721 catcggcgca gccgtgctgg atgacatcct gggcgtgctg gcgctggctt ttctttacca
     6781 gttcgcggtg gaaaaacagg tttcggttca ggcgctgggc gaagttagcc tgtatatcct
     6841 gctgttcatg ctgctgacgc cattggccgc caagctggcg gcgggaatca tcgaccatct
     6901 ggatcgccgc gccgcgactc ccggcttgct gctgaccatg gcgctgtcat tgatcctgct
     6961 gttctcctgg ctggcgcatc tggtgggagc gccggtcatc atgggcggct tcgctgctgg
     7021 cctggcgttt ggccagcatt tcggccttaa cctgaaaatc cgtttgccgt tcgggcgcga
     7081 gttgtcgttt acacccagcc ccaagctggc acaccgattg gaagagcaaa tgcgcccgct
     7141 gatccacacc tttgcgccgc tgtttttcgt catggtcggc gtatcgctca acctcagcgc
     7201 agtggactgg gactcggcgt tcatctggga actggccacg ctgctgctaa tcgccgcctt
     7261 catcggcaag ctcgccgccg gctattgcat ccgtgaacca cgccgcgcgc aaacggcgat
     7321 tggcctggcg atgatcccgc gcggcgaagt gggactgatt ttcgcccaat tgggttttac
     7381 ccagaacatc cttaacggcg aactctacgc cgccatgttg atcgtcatcg ccctgaccac
     7441 catggcacca ccgtttttgc tcaaatggtt ctacggcagt ccttctgtga ccggttgatt
     7501 ctggtccaat cgaagaatgg cccgggatcg gtcttgcggc cgggtgcgat gtcggaatga
     7561 ccaacgatgc cagcaatcgg gtagcgttgt tcgatcgctg tcagcaaggc ttccagggtt
     7621 gcatactgca catcctcgaa ccgcgtggtg tcgtcgcctt ccagttcgat gccgatagag
     7681 aagtcgttgc agcgctcccg cccttgccat tgtgatacgc cggcatgcca ggcgcgcttt
     7741 ccacacggca caaactggat cacttcgccg ctgcggcgga cgaagaaatg cgaggatacc
     7801 ttgaactggt gaatttcggc gtagtaggga tgttcggcgg gatccagccg gttggtgaac
     7861 agctcaacca cgccgtcgcc gccgaactcg cccggtggca ggctgatatt gtggatgaca
     7921 accaagtcga tcaccgtacc gatggggcgt tcgtcggagt tgggcgaagt gatctgcaga
     7981 gcctggcgtg cggtaccggt gtcgtcaagg ttaaacatgc gcatgccgtc tgcttccgat
     8041 aaaatcgtgg cttggtactg aatccggagc tctcatggta ttcctcgaac tgttcgtcat
     8101 gctggtggtg attctagtcg ctgccgaagt cttcaccaat gccttggagc acctgggcga
     8161 aaaactgaaa atctccgaag gcgtcaccgg ttcgctgttc gctgccgtcg gtacggcact
     8221 accagaaacc ctggtgccac tgctggcgct gctggccggc accaaaaacg tacaggtcaa
     8281 cgaggaaatc ggcgtcgggg ccatccttgg cgcgccattg atgctctcca cgctatccct
     8341 ctcgctgctg gcgttcgccg tattgcgcaa gcgtggccct cacggcgagt tccgcccgga
     8401 aaggaccggt ctattgcgcg atctcgattt ctttcttgcc gcctttgcct tcgccaccct
     8461 ggcgctgttc atcccgcata ccatgaccga agtgcgtgcg gcgcttggat tcaccatggt
     8521 actgatctat ttcatctaca ttatgctgac cttgcgtgcc tccaaggcgc tggtgcacga
     8581 tggccacgcc accgaggccg aagagccgat gtttctctgc cgcgccggcc tgcccaacaa
     8641 catgctggtg atcctcctgc agttggccgg ggggctggcg ttgctggtat atggcgcaca
     8701 tggcttcatc catggcgtcg aggctgccgc cccgctgctc ggtctttcgg cgctgctgct
     8761 gtccctgctg atcattccga ttgccaccga actgccggaa aaggtcaaca gcatcctgtg
     8821 ggtgcgcaaa cacaaggaca cgctggcgtt tagcaacatc accggcgcca tggtgttcca
     8881 gggcacgctg ctgccggcga tcggcatcat gctgacgccg tgggaaccgc gccgcgaggt
     8941 gctggccggc gtggtcatca ccctgctggc cgccgcctgg ttgcgctgga tggcgagccg
     9001 tggccacctc aaggtgtgga tggtatggat caatggcgcg ctgtacctga cctacctggc
     9061 ggtggcgctg gcctgaaccc gcactagatc tcgtcctcct tgtcgtcttc cctgctcagc
     9121 cccagcttgg ccagccggta gcgcaaggct ctgaaactga cgcccagaag tttggcggcg
     9181 gcggttctat tgttcccgga tttttgcaag gcgtcgagaa ttgccttttt ctcgatgctt
     9241 tccagatatt ccggcagcga attcattgcg gcagacgccg cggcctcact gtcctgcgat
     9301 actagctcga gcagcaggtc atcggcttcg atgcgtttgc catcgcatag cgccagtgcg
     9361 cgttccagca cgttttccag ctcgcgcacg ttgcctggaa aagcgtactg cgtcagcagc
     9421 gtcatggcgg tagcggaaag ctccggctga gcagcgccta attcccgact gtgcttctcc
     9481 agaattttcc ccgccaattg cggaatgtcc tcctgcatgt cgcgcaagga gggcatcttg
     9541 agctcgatga cgttcagccg ataataaaga tcctggcgga attggccggc ttccatcatc
     9601 ttgcccagat tacggtgcgt ggcgctgatg atgcgcacgt ccaccgcctc ttcctgggca
     9661 ccaccaacca tgcgcacttt cttctcctgg atagcgcgca acagcttgac ctgcatgtgt
     9721 agcggcaggt cggccacttc atccaggaac agcgtgccgc cgttggccac atgaaaaacg
     9781 ccgggtttgt cctgctgcgc accggtgaag gccccttttt tgtagccgaa aaattcgctc
     9841 tccatcagcg tttccggaat ggcgccgcaa ttgaccggaa caaaaggccc gtcaccgcgc
     9901 gagcttttgg catgaatcag ccgggccgca agctccttgc cgctgccgga ttcgccactg
     9961 atatagaccg gcgcctgact gcgcgcaagt ttatcgatca tcaggcgcac ctgtcgtacc
    10021 ggcgcggaat caccgatcaa ggccaactcg ccctcgtctg ctgcgtgcgg ctccgccaac
    10081 ctgagcgccg actgtaccag cggccgcaat tgatcgagcg aaatcggttt ggacaaataa
    10141 tcgaaagcgc ccgccttcag cgcggcgacg gcattttccg cgctaccgta ggcggtgatc
    10201 acggcaaccg ggagtcccgg gcaagtctgg ccgatatgcc gcaccagatc caggccattg
    10261 ccgtccggca agcgcatgtc ggtaaggcac aatccgaagt cgtgccgttg cagcaaatgg
    10321 cgcgcttcct ccagggtacc ggcacccatc gcgggcacat ccatgcgttc cagggtcaac
    10381 agcagcaatt cacggatatc ggtttcgtca tccacgacca ggcaggcatg catgttgcgt
    10441 gatttattgg acatgatgct tcttcaggta aatgttgaac tgtccgccag catccgatcc
    10501 ggcatatccc agactggcgc cgttcgcttc acacagctcg cgggcaatat agaggccgag
    10561 cccggtaccg ccgctttcgg tcgtgaagaa cggctcgaac agatggggac gcacctcgtc
    10621 cggcacgccg tcaccgtcgt ctttcactgc gatcacgatc tcttcttttc tgcgtgactg
    10681 ttccaggcgc agacaaagac ttcccgcgcg cttccgtcca tgccgccagc cgttacggca
    10741 cagattccac aacacctgat gcagatggcg ccggtcgaac aaggcgctgg catcctcggc
    10801 aatatccagc gccaaatctt ccggtggaat attttccacc tggcagaact gctcacgaaa
    10861 ctcggacaga aaatccggca gatgaatgat ttcctgctgc aagcggtcgc gctggttcaa
    10921 ttgcagtacg tccttgacga tctgatccag cctgacgacg ttgtcgttga tgatctgcag
    10981 caagcgctgg tgggtcacat cctgaccacg atcctcctgc agcaactggc tggcatggct
    11041 gatggcgctc aggggattgc ggatttcgtg cgcgacgttg gccgtcaacc gccccagcga
    11101 ggccagcttg agctgtctgg cctgggattg cacgcggtcc cagtcttcga taaaaatgac
    11161 agcgccctgg ctgcgatccg ctccgaccgg ctggaagcgc aatcgcagct ccttgcttcc
    11221 cgcggttacc ctgacctcat tggctctggc atcatttcgg gctttaagcc attgacgaaa
    11281 caaggccgcg atttcgctag aaaagccttc aagagtcggc gccgtgacat cgctgacgag
    11341 cccgcccagc atagcctcgg cctgagcgtt atgatggcgc agtcgcaaat ccttgtccac
    11401 gatcagtacg ccatccagca tgtcgcgaat gacaatctgg ttgaccagcg cctgattttc
    11461 cagatcgata ccccgctgcg aagccagttc ttcgctctgg cgggtacgct gggccaggct
    11521 gtgcgccagc caggctgtgg cgaagcagct caggccgagc atgacgacat gcgagtaact
    11581 ctcgaagctt tcgtccagag cgagaatccg ccaggactgc tccagcagca aggcaatggt
    11641 ggccagcgcc gcatagaata gcgccaggcg accctggctg atcagcgcgg ccgcggcgat
    11701 cgctatcacc agcagcaaac ccaaaccgct cttgacaccc cctgccgcgg acatcagcaa
    11761 cacaatgaag ccaatatctc caatgacctg gccggtcaac tgccggttga agcgcgggag
    11821 acgaagtctg gtaaagaata tggcggccag gctgaagacc acgtaagccg aggctgcctg
    11881 caaatagagc gtgttgtcgt agttttcccg caagacacgc tctgacatca ggaaatacgt
    11941 gcccagcaat ataccggaca ccaccagccg gtacagcgtg aagtagcgca tggtgcgcca
    12001 gtaggcatcg gggtgaccgg cggggtggct tggctccggc aaagtcattg ctttgattgc
    12061 ctgctgtgct cttcgcagca atagtagccg ccgcgcgacg ccacgctctc cgactttggc
    12121 agatgcacgc cacatacccg gcactgcacc atgtcctgcg cgcccggact ttttggggcg
    12181 gggcggtcca tattgcggcg gtactgcccg agaatgaaga ccagcagcca gacggcgaga
    12241 gcgagcagaa tgagttttcc cagcatggca agtccttgag taaggttagc ggattgtagc
    12301 gaaaagcccg gcacactcaa acccgctccg acatatcgtc tcgtgtccat tggcaatgtc
    12361 cggcgcaagc acatacaata acccggaatg gcaaccccac tggaactttc ggattttctg
    12421 gcgcagaccg agcggcgggc gttcaggcaa gccgcctacg cggtgcggga cgatcacgtt
    12481 gcgctggaca ttgtccagga cgccatgctc aagctagccg agaaatatac tgcgcgacca
    12541 gtcacggaat atcccatgct gttccagcgc atcctgcaga acaccatccg cgaccacttc
    12601 cgccggcaga aagtacgcaa tctctggacg acgctgcttt cctcgttttc tacgggagag
    12661 gatggcgatg agcacgatcc gctggagacc ctgcaaaccg gggatgacgg tcaggacgat
    12721 acgcgcgagc agatcgagcg caagcaaacg ctcgctatca tcgaagccgc cttgcaacgg
    12781 ctgccgacgc gtcaacgcga agccttcgtc ctgcgttact gggaagattt ggacgtagcg
    12841 gagaccgcgg cggttatggg atgctcggaa ggtagcgtca agacgcactg ctcgcgtgcg
    12901 gtgcatgcgc tggccgcctc gctgaaaacc aaagggatca gcttatgaca tcagaacaaa
    12961 agatcgcaaa agaaatcgtc agcctgctgg attacgggac gacgcaagtg gatcaagcgg
    13021 cgtccgaccg gctatatgcc gcccgtcggc aggcggtagc ggtagcgcag gccaggattt
    13081 cccccgttgc atctaccggt gccgggaatt tcctgctgga gcacctgcac ggccagcaag
    13141 catggaagtt accgctgctg ttgctgggtg tagtactggc agccttgctg ttgacccagc
    13201 aaataaccga gagcgggccg gttgaggagg atgccctgtt gctcggttcc gatctgccgc
    13261 cggaagcata cctggataaa ggtttcgatg catggctaga accctcctcg cagcattgat
    13321 cctgtcttcg gctatcgggg tggcaaatgc ccgcgccgag ggcgcgcccg tcgccgagcc
    13381 tcattggtcg caactgagca atgagcagaa acaggtgctg gcgccgcttg cctcgcagtg
    13441 ggatactctg cgcccgtggc agagagagag aatgctggat attgcacgcg attacccacg
    13501 catgcccccg caacagcagg gtcgcgtgca acagcgtttg cgtgactgga gccgcatgac
    13561 gccgttcgag cgggaaaacg cgcgcaagaa gtatcagcaa tttcagttgc tgccgccgga
    13621 aaaacaggaa gaaatgcgcc gcaaatggag cgactatcaa cggctcccag aagccgagcg
    13681 ccagaaactg cgcgaaaagc aaccggaatc caccccggtg gat
//
LOCUS       DAOMFA010000025        13664 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_396_length_13664_cov_5.256374, whole genome shotgun sequence.
ACCESSION   DAOMFA010000025 DAOMFA010000000
VERSION     DAOMFA010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 13664)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13664)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13664
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_396_length_13664_cov_5.256374"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(108..713)
                     /gene="coaE"
                     /locus_tag="PKW44_02395"
     CDS             complement(108..713)
                     /gene="coaE"
                     /locus_tag="PKW44_02395"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831464.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HQR50471.1"
                     /translation="MFIVGLTGGIGSGKSEAARMFAALGVPVVDTDAIAHGLTSPGSP
                     LLAEIAQALGRDCIKPDGSLDRAWLRRCVFADADARRALENILHPRIRETAAQLLAQH
                     ADTPYQIVVVPLLFETGGYAGLVSHSLVVDCDEALQITRTMQRNPLSEAEVRAIMAAQ
                     LPRSQRVALADDVIENDGSLKKLEESVRAKHEKYIKTCIVS"
     gene            complement(713..1582)
                     /locus_tag="PKW44_02400"
     CDS             complement(713..1582)
                     /locus_tag="PKW44_02400"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019899549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HQR50472.1"
                     /translation="MLENPLQVANLLREQPAFFIAVSVVFGLLVGSFLNVVIHRLPRM
                     MEQEWRQQCAELNGQETAKSVRYNLVTPRSACPACGHNIGAWENIPLLSYLALGGKCS
                     ACKIHISARYPLVEGLTGLLAGLIAWRFGFSGMTVAAWLFAFSLIALTFIDFDTQLLP
                     DDITLPLIWAGLLYNLDGGFASLSSAVTGAVAGYLMLWSIYWLFKLATGKEGMGYGDF
                     KLLAAIGAWFGWQMLPAVILLSSLGGSVIGIGLIVLARHGRNVPVPFGPYLALGGIAA
                     LFWGPQLAHLYLL"
     gene            1710..2027
                     /locus_tag="PKW44_02405"
     CDS             1710..2027
                     /locus_tag="PKW44_02405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR50473.1"
                     /translation="MLESVTPAFFDHGIVCQAVVDLPAWLEDLSGRSGWRLEREEEGE
                     LCDSYAFRRGAEEIQVVLYHTGHATVENSETVLYDGHLSMAAGYARLQYFNAENGERV
                     LLN"
     gene            2244..2663
                     /locus_tag="PKW44_02410"
     CDS             2244..2663
                     /locus_tag="PKW44_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PsiF family protein"
                     /protein_id="HQR50474.1"
                     /translation="MNKLIAVAVALMLSCPLAFAGTQQDKMKTCNAEAGKKSLAGDER
                     KAFMKGCLSADAPAPAAGAVQQDKMKTCNADAGKKALAGDERKAFMKDCLSADSGKSA
                     AGTSQQDKMKTCNADAGKKALKGDERKAFMKDCLSAK"
     gene            complement(2757..3335)
                     /gene="slmA"
                     /locus_tag="PKW44_02415"
     CDS             complement(2757..3335)
                     /gene="slmA"
                     /locus_tag="PKW44_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HQR50475.1"
                     /translation="MATKPGERKQQILETLAKMLEAPKREKITTAALAARLDVSEAAL
                     YRHFAGKAQMFEGLIEFIEQSLFGLINKISAEETDGLKQVRRILTVLLLFAEKNPGMT
                     RVLIGDALVNEDDRLQARINQLFDRVESTLKQSLRIAETQTGRRLDPEAQANLLICFV
                     IGRWQQFAKSGFKRKPMEFVQPQLGLLYGLEE"
     gene            complement(3363..4037)
                     /locus_tag="PKW44_02420"
     CDS             complement(3363..4037)
                     /locus_tag="PKW44_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HQR50476.1"
                     /translation="MQRVWIFDLDDTLHNASAHVFQHINRSMTHYLMTHLDMDEQAAC
                     ELRRHYWQRYGATLHGLMRHHGTDPHHFLHHTHQFPQLESMVLKTRGLRHALRRLQGR
                     KVVFTNAPMIYAEQVLRLLKIRDLFEAVFSIESTRFQPKPARQGFQRLLRRLGVKVER
                     CVMVEDSLPALATAKRLGMKTVFVNPGPAKPRYVDVRIGSVLELPATALTPAFSHLRE
                     KGTMCR"
     gene            complement(4066..4950)
                     /gene="argB"
                     /locus_tag="PKW44_02425"
     CDS             complement(4066..4950)
                     /gene="argB"
                     /locus_tag="PKW44_02425"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019897300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HQR50477.1"
                     /translation="MLTTTTAAQKAQTLAEALPYIKRFFDKTIVIKYGGNAMTDPALQ
                     DCFARDVVLLKLVGMNPVVVHGGGPQINELLDRLGKKGTFIQGMRVTDEETMDVVEMV
                     LGGQVNKDIVNLINRHGGKAVGLTGQDGNFIRAKKLLMENKEIPGEFIDIGQVGDITG
                     IDPSIIAFLDTGDFIPVIAPIGVGPDGETYNINADVVAGKLAEILGAEKLILLTNTPG
                     VLDKKGELLTGLTAKEIDGLFEDGTRSGGMLPKISSALDAARSGVKSVHIIDGRVEHA
                     LLLEVLTDEGVGTLIRAK"
     gene            5056..6543
                     /locus_tag="PKW44_02430"
     CDS             5056..6543
                     /locus_tag="PKW44_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HQR50478.1"
                     /translation="MNEIVEQQGYAERRQALLQGLRQIVPPACVLSEPEALRPYECDG
                     LSAYRQLPGVVCLPETLEQAQAVLRLCRRLQVPVVPRGAGTGLSGGALPHAEGLLLGL
                     ARLDRIIEIDPLNRCAVVQPGVRNLALSEAARPHGFYYAPDPSSQIACTIGGNVAENS
                     GGVHCLKYGLTVHNVIGVKLLTMQGELLELGGKALDATGYDLLALVCGSEGLLGVVVE
                     VTVRLLPLPERAQVVLAAFDDVAVAGEAVGRIISAGIIPAGMEMMDNLAICAAEDFVH
                     AGYPTDAAAILLVELDGENREVSEQIFRVRTLLLESGASEVRTARDEAERLLFWKGRK
                     AAFPAVGRMTPDYYCMDGTIPRRELPRVLRRIAELSQEYGLPVANVFHAGDGNLHPLI
                     MYDANHPDELARTEELGGKILQLCVEVGGTITGEHGVGVEKIDAMCSQFGAAELAQFH
                     VLRSAFDPDRLLNPGKAVPTLHRCAELGRMHVHGGQLPHPELERF"
     gene            6543..>6680
                     /locus_tag="PKW44_02435"
     CDS             6543..>6680
                     /locus_tag="PKW44_02435"
                     /inference="COORDINATES: protein motif:HMM:NF008439.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR50479.1"
                     /translation="MEQRLAETIRAAAAERRQLAIRGSGSKTFYTGVAQGEILYTRGL
                     RG"
     assembly_gap    6681..6690
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6691..7586
                     /gene="glcE"
                     /locus_tag="PKW44_02440"
     CDS             <6691..7586
                     /gene="glcE"
                     /locus_tag="PKW44_02440"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011310559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HQR50480.1"
                     /translation="VSYQPSELVLTARTGTSLVEIEAALTEQGQMLACEPPHFGDGAT
                     LGGVVACGFSGPRRPWGGAVRDFVLGVRMVNGQGEVLHFGGQVMKNVAGFDVSRLMAG
                     SLGSLGLLLEVSLKVLPRPAYEITLRRECTQAGALAQCAAWAARPLPLSAAAWLNGAL
                     YVRLSASEAAVRAAAQATGGEAVPDGTAFWQSLREHAQPFFTLAEGETLWRLSLPPAA
                     PVSDLAGRWCDDWGGAQRWLVTAQDAEAVRQAACGAGGHAVPFRGAQAGQLQLAPGLA
                     ALHDRLKAAFDPHGVFSPVAR"
     gene            7586..8788
                     /gene="glcF"
                     /locus_tag="PKW44_02445"
     CDS             7586..8788
                     /gene="glcF"
                     /locus_tag="PKW44_02445"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HQR50481.1"
                     /translation="MQTRLPPVLQQTAEGREAEAILRACVHCGFCTAVCPTYRLLGNE
                     LDSPRGRIYLIKSLLEGEAIGEQTRTHLDRCLTCHACETACPSGVQYARLADIGRAAL
                     ERQVPRPWPQRLWRWLLRRTLASPRVFAGLLAIGRTARPLLPAPLKRRIAPATVAGSW
                     PPPRHARRILAPAGCVQPALAPGIDAAMARLLDAHGVSLVKVDTGCCGALDQHLAAPE
                     QALHRARVYVDVLWPQIEAGAEAVAVSATGCGAMVREYAHLLRDDSAYAARAARVSAL
                     CRDPVELLETLPVADVGRGRRIAFHSPCTLQHALRLEGRVEALLQRAGFILLPVEEAA
                     QCCGAAGTYSILQPGLSEQMLQRKLGYLQQHAPELIATANIGCLTHLQGGGEMSVVHW
                     VELLAEGD"
     gene            8944..9081
                     /locus_tag="PKW44_02450"
     CDS             8944..9081
                     /locus_tag="PKW44_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008059032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR50482.1"
                     /translation="MLWVLFYATAVITLILYFTGFLTRHNLEWLVLVLAASVFPAVIY
                     L"
     gene            complement(9155..9586)
                     /locus_tag="PKW44_02455"
     CDS             complement(9155..9586)
                     /locus_tag="PKW44_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HQR50483.1"
                     /translation="MANVTRFDPFQISALDPFDDVFKGFFRPVRIEGAPDIQIKMDVK
                     EDDKGYTVHAEIPGVKKEDIHVTIDGNQVSISAEAKQEKEVKEGEKVLRSERYYGKVA
                     RSFSVASDIDEAASQARYADGVLELALPKKTVASSKKLTIN"
     gene            complement(9824..11149)
                     /gene="hslU"
                     /locus_tag="PKW44_02460"
     CDS             complement(9824..11149)
                     /gene="hslU"
                     /locus_tag="PKW44_02460"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HQR50484.1"
                     /translation="MSQMTPKEIVHELDKHVVGQAAAKRAVAIALRNRWRRAQVAEPM
                     RQEIKPKNILMIGPTGVGKTEIARRLARLANAPFIKVEATKFTEVGYVGKDVDSIIRD
                     LAEIAVKQTREQEMEKVRIRAADAAEDRILDILLPPARSLNEESAHADSETRQKFRKM
                     LREGKLDAKEIDIELAAPTAHMEIFSPPGMEELTSQIQNAFQGMGGGKPRLRKLKIAD
                     AMKHITDEEAAKLVNEDDIKLHAMHNVEQNGIVFLDEIDKITSRSESSGAEVSRQGVQ
                     RDLLPLVEGTTVTTKYGMVRTDHILFIASGAFHLAKPSDLIPELQGRLPIRVELDSLS
                     VGDFEQILTGTDACLTRQYQALLETENVTLAFKPDAVRRLAEIAWQVNEHTENIGARR
                     LHTVMEKLLEEVSFDADKHADGMLAIDAGYVNAKLQGLAQDEDLARYVL"
     gene            complement(11155..11691)
                     /gene="hslV"
                     /locus_tag="PKW44_02465"
     CDS             complement(11155..11691)
                     /gene="hslV"
                     /locus_tag="PKW44_02465"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HQR50485.1"
                     /translation="MEQYHGTTILSVRRGDKVAMGGDGQVTLGNIVVKATARKVRRLY
                     HGNILAGFAGGTADAFTLFERFEAKLEKHQGNLLRSAVELAKDWRTDRMLRRLEAMLT
                     VADKESSLIITGNGDVLEPEYGLLAIGSGGAYAQSAARALLDHTDLPSAEIVKKALTI
                     AADLCIYTNQNHIIETLE"
     gene            11804..>13664
                     /locus_tag="PKW44_02470"
     CDS             11804..>13664
                     /locus_tag="PKW44_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HQR50486.1"
                     /translation="MQVSPAIRVIAMPNRLASETSPYLRQHADNPVDWYPWGEEALAL
                     ARQQDKPILLSIGYSACHWCHVMAHESFEDPATAALMNELFVNIKVDREERPDLDQIY
                     QSAHAILSRRGGGWPLTMVLTPEQVPFFGGTYFPPAPRYGMPGFSDLLRQIAMAWRER
                     RADIEAQNAEVLSVLVKMVRQDAPAALDGAVVTAAVSQLAQMFDSVNGGFGGAPKFPN
                     PADLALLLRRASAGDPQACHMVTLTLSKMADGGIYDHLGGGFCRYSVDDHWEIPHFEK
                     MLYDNGQLLSLYSEAWLMTGDNQYREVVGHTVDWLLREMRAPDGAFYSSLDADSEGEE
                     GRFYAWQRDEVRRLLDAPEYALLEKRYGLDSTPNFEHTWHLHVTSDSETAARQLGLTP
                     QQSANLWQSARAKLWRVRELRVRPGRDDKILTSWNALVITGMARAGRAFGRPDWILAA
                     QQAVDFIHTQMWREGQLLATCKDGRAHLNAYLDDHAFLLHALLELMQAEFRRTDLDFA
                     VEIAEALLARFQDDEHGGFFFTSHDHEALIQRPKSVHDNATPSGNGVAAYALQRLGHV
                     VGEARYLAAAEKTLRAFAGTMQRNPAASPSLVAAFDEYLAPPALLYLRGPQEKL"
BASE COUNT         2414 a         4065 c         4436 g         2739 t
ORIGIN      
        1 agccgcagta gcgtgcgtat gcgttcgttg aatgggtagt cgtagctggc cactaattaa
       61 ccacgaagta gtagaaggaa tgtaattacc gtggattatg aaattgatta gctaactatg
      121 caagtcttaa tgtatttttc atgttttgcc cgcactgatt cctccagttt tttcagggaa
      181 ccgtcgttct cgatgacatc atccgccagc gccacgcgct ggctgcgtgg taactgtgcc
      241 gccatgatgg cgcgtacttc cgcttcggac aaagggttgc gctgcatggt gcgggtgatt
      301 tgcagcgctt cgtcgcagtc cacgaccaga ctgtggctga cgaggccggc atagccgccg
      361 gtttcaaaca gcaagggaac cacgacgatc tggtaaggtg tgtcggcatg ctgcgcaagc
      421 agttgtgcgg cggtttcgcg gattctggga tgcagaatat tttccagcgc gcggcgcgcg
      481 tcggcgtcgg caaaaacgca ccggcgcagc caggcccgat cgagcgagcc gtcgggtttg
      541 atacagtcgc ggcccagggc ttgtgcaatt tccgccagca gcgggctgcc gggtgaagtc
      601 aggccgtggg cgatggcatc ggtatccacc accggcaccc ccagtgcagc gaacattctg
      661 gccgcttcgc ttttgccgct gccgatgccg cctgtcagtc cgacgatgaa catcaaagca
      721 aatagaggtg agccagttgc ggcccccaga acagcgcggc aatgccgccc agcgccagat
      781 aggggccgaa cgggaccggc acgttgcggc cgtggcgcgc cagcacgatc aggccaatgc
      841 cgatcaccga accgcccagc gatgacagca ggattaccgc cggcagcatc tgccagccga
      901 accaggcgcc gatggcggcc agtagcttga agtcgccgta gcccatgcct tctttgccgg
      961 tggccagctt gaacagccag tagatcgacc acagcatgag atagccggcc acagcgccgg
     1021 tcactgccga agacagactg gcgaacccgc cgtcgagatt gtaaagcagg ccggcccaga
     1081 tcagcggcaa cgtgatgtcg tccggcagca gctgggtatc gaaatcgatg aaggtcaacg
     1141 cgatcaggga aaaagcgaac agccaggccg ctaccgtcat gccgctgaaa ccgaagcgcc
     1201 acgcgatcag cccggccagc aacccggtca gcccttctac caaagggtag cgggcgctga
     1261 tgtggatttt gcaggcggag catttgccgc ccagcgccag atagctcagc agcgggatgt
     1321 tctcccaggc gccaatgttg tggccgcagg ccgggcaggc cgagcgcggt gtgaccaggt
     1381 tgtagcggac ggatttggcg gtttcctgac cgttcagctc ggcgcattgc tggcgccact
     1441 cctgctccat catcctgggc aggcggtgga tgaccacgtt gaggaagctg cccaccagca
     1501 ggccgaatac caccgaaacg gcgatgaaaa aggccggctg ctcgcgcagc agattggcca
     1561 cttgtagcgg gttttcaagc atggcgcatc atcccatcgt tgccgggcct tggcaaggca
     1621 acgggatgaa ggaagccctt gaaatagggg agtccgccgc catataacta acgaacggca
     1681 cggaaagtgc caccaaggag ctgaacatca tgctggaatc tgtgacccct gctttttttg
     1741 accacggcat cgtctgccag gccgtggtcg acctgcctgc ctggctggaa gacctcagtg
     1801 gaagatcggg ctggcggctg gagcgcgagg aggagggcga gctgtgcgat agctacgcct
     1861 tccgtcgcgg cgcggaagaa atccaggtgg tgctctacca caccggccat gcgaccgtcg
     1921 aaaacagcga gaccgtgctc tacgacggtc atctgagcat ggccgccggg tacgcccgcc
     1981 tgcagtattt caacgcggaa aacggcgaac gggtattgct gaactgatat gtaaaagtcg
     2041 tagaaggggt gccttcgccc cgatggtctt ttgtcatcgc gccgagcgcg gcgctcctgc
     2101 aggctggcgc ttgcaacgca gcagttggcg gtgagcgaca gcgtaagtac acctgggaca
     2161 tcggtccttg cgccgcgaag tatcttcttc tggagggctt ggggtaagat ggctgttccc
     2221 ccaaccagca aggagtttca gacatgaaca agttgattgc cgtcgccgtc gcattgatgc
     2281 tctcctgccc gcttgccttc gccggcacgc aacaggacaa gatgaagacc tgcaacgcag
     2341 aagcgggcaa gaaatcgctg gcaggcgatg aacgcaaagc cttcatgaag ggctgtctct
     2401 cggccgatgc gccagcgcct gcggctggcg ccgtccagca ggacaagatg aaaacctgca
     2461 atgcagatgc gggcaagaaa gcactggcgg gcgacgaacg caaggccttt atgaaagact
     2521 gtctgtccgc cgactcggga aaatccgcgg ccggcacatc ccagcaggac aagatgaaga
     2581 cctgcaacgc cgatgcgggc aagaaggcac tcaagggcga tgaacgcaag gccttcatga
     2641 aggactgcct gtcggccaag taattcgcac tgcaaaaaag agaaacggga ttgcggtgac
     2701 gcaatcccgt tttttctgga cggcagccct ttatgcttgt tcagcgcctt ctcaagctac
     2761 tcttccagcc catacaacaa ccccaattgc ggctgcacga actccatcgg cttgcgcttg
     2821 aagccgctct tggcaaactg ctgccagcgg ccgatgacga agcagatcag caggttggcc
     2881 tgggcttcgg ggtccagcct gcggccggtc tgggtttcgg cgatgcgcag gctttgcttg
     2941 agggtggatt cgacgcggtc aaagagctgg ttgatgcgcg cctgcaggcg gtcgtcctcg
     3001 ttgaccaggg catcgccgat cagcacgcgg gtcatgccgg ggtttttctc ggcgaacagc
     3061 agcagcacgg tgaggatgcg gcgcacctgt ttgaggccgt cggtttcctc tgcgctgatc
     3121 ttgttgatga ggccgaacag gctttgttcg atgaactcga tcagtccctc gaacatctgc
     3181 gccttgccgg cgaagtggcg gtaaagcgcg gcttccgaga cgtcgagcct ggccgccagc
     3241 gcggcggtgg tgatcttttc gcgcttgggg gcctccagca ttttggccag ggtttccagg
     3301 atttgctgct tgcgttcgcc gggtttggtt gccatgggag ttccttcaaa agttaagtcg
     3361 cgtcagcgac acatcgtccc cttctcccgc aagtgggaga aggcaggggt gagggcggtt
     3421 gcggggagtt ccagcacgct gccgatgcgg acatccacat agcgcggctt ggctgggccg
     3481 gggttgacga aaacggtttt catgccgagt cgcttggcgg tggccagcgc cggcaggctg
     3541 tcttccacca tcacgcagcg ttcgactttg acgccgagtc tgcgcagcag gcgctggaag
     3601 ccctgccggg cgggcttggg ctggaagcgg gtggactcga tgctgaacac ggcctcgaac
     3661 aggtcgcgga ttttcagcag gcgcagcacc tgctcggcat aaatcatggg cgcgttggtg
     3721 aacaccacct tgcgcccctg cagccgccgc agcgcgtgac gcaggccgcg cgttttcagc
     3781 accatggatt ccagttgcgg gaactggtgg gtgtggtgca aaaagtggtg cgggtcggta
     3841 ccgtggtggc gcatgagtcc atgtaaagtc gcgccgtagc gctgccagta gtggcggcgc
     3901 agttcgcagg cggcctgttc atccatatcc aggtgcgtca tcaggtagtg cgtcatgctg
     3961 cggttgatgt gctggaacac gtgggcgctg gcgttgtgca gggtgtcgtc aaggtcgaaa
     4021 atccagacac gctgcaatta cttggcccgg atcagcgtgg acatattatt tggcccggat
     4081 tagcgtgccg acgccttcgt cggtcagcac ttccagcagc agggcgtgct cgacccggcc
     4141 gtcgatgatg tgcaccgatt tcacgccgct gcgcgcagcg tccagtgccg agctgatctt
     4201 gggcagcatg ccgccggacc gcgtgccgtc ctcgaacagg ccgtcgatct ccttggcggt
     4261 gaggccggtg agcagctcac ccttcttgtc cagcacgccg ggggtgttgg tgagcaggat
     4321 gagtttttcc gcgccgagga tttccgccag cttgccggcc actacgtcgg cgttgatgtt
     4381 gtaggtctcg ccgtccggcc caacgccgat cggcgcgatg accgggatga agtcgccggt
     4441 gtcgagaaag gcgatgatgc tggggtcgat gccggtgatg tcgcccacct ggccaatgtc
     4501 gatgaattcg ccggggattt ccttgttctc catcagcagc ttcttggcgc ggatgaagtt
     4561 gccgtcctgg ccggtcaggc cgaccgcctt gccaccgtgg cggttgatca ggttgacgat
     4621 gtccttgttg acttggccgc ccagcaccat ctccaccacg tccatggttt cctcgtcggt
     4681 gacgcgcatg ccctggatga aggtgccctt cttgcccagc cggtccagca gctcgttgat
     4741 ctgcggcccg ccgccgtgga cgacaaccgg gttcatgccg accagcttga gcagtaccac
     4801 gtcgcgcgcg aaacagtcct gcagcgcagg gtcggtcatg gcgttgccgc cgtacttgat
     4861 gacaatggtc ttgtcgaaga aacgcttgat gtagggcagt gcttcggcca gggtttgcgc
     4921 cttctgtgcg gcggtggtcg tggtgagcat ttacttttcc gggaaaggtt cgaattggtg
     4981 ggtattttat acggaaattg ttgcaatggc agggacatgt ttatcctccc tcacccgtgg
     5041 taatctttcc cggcgatgaa tgaaattgtc gaacaacaag gctacgctga acgccgtcag
     5101 gcgctgctgc aaggcttgcg ccagatcgtg ccgcccgcct gcgtgctgag cgagccagag
     5161 gcgctgcgcc cctacgaatg cgacggcctt tccgcctacc gccagttgcc cggcgtggtg
     5221 tgcctgccgg aaacgctgga gcaggcgcag gcggtgctgc gtctgtgccg gcgcttgcag
     5281 gttccggtcg tgccgcgcgg cgccggcacc gggctttccg gcggcgcgct gccgcatgcc
     5341 gagggcctgc tgctgggact ggccaggctg gaccggatca tcgaaatcga cccgctcaac
     5401 cgctgcgccg tggtgcagcc cggcgtgcgc aacctggcgc tgtcggaggc ggccaggcct
     5461 cacggcttct attacgcccc cgacccttcc tcgcagatcg cctgcaccat cggcggcaat
     5521 gtggcggaaa actccggcgg cgtgcactgc ctcaaatacg gcctcaccgt ccacaacgtc
     5581 atcggcgtga agctgctcac catgcagggc gaactgctgg agctcggcgg caaggccctg
     5641 gacgcgacgg gctacgatct gctggcgctg gtgtgcggct cggaagggct gctgggcgtg
     5701 gtggtggaag taacggtgcg cctgctgccg ctgccggagc gcgcccaggt ggtgctggcg
     5761 gcgttcgacg atgtggcggt cgccggcgag gcggtggggc gcattatttc cgccggcatc
     5821 atcccggccg gcatggaaat gatggacaac ctggccattt gcgccgccga ggattttgtc
     5881 catgccggct atccgacaga tgctgccgcc attctgctgg tggaactgga cggcgagaac
     5941 cgcgaggtgt cggagcagat tttcagggta cgcactctgc tgctggaaag cggtgccagc
     6001 gaagtgcgta ccgcacgcga cgaggccgaa cgcctgctgt tttggaaggg ccgcaaggcc
     6061 gcttttcccg ccgtggggcg catgacgccg gattactact gcatggacgg caccatcccg
     6121 cgccgggaac ttcccagggt cttgcggcgc atcgccgaat tgtcacagga atacggcctg
     6181 ccggtggcca acgtgttcca tgccggcgat ggcaacctgc acccgctcat catgtacgac
     6241 gccaatcacc cggacgaact ggcgcggacc gaggaactgg gtggcaagat attacagctg
     6301 tgcgtcgagg tcggcggcac catcaccggc gagcacggcg tgggcgtgga gaaaatcgac
     6361 gccatgtgca gccagttcgg cgcggcagag ctggcacagt tccacgtcct gcgcagtgcc
     6421 ttcgaccccg accgcttgct caacccgggc aaagccgtac ccaccctgca ccgctgcgcc
     6481 gaactgggcc gcatgcacgt acacggcggg cagctgccgc atccggagtt ggagcggttc
     6541 tgatggaaca gcgcctggcc gaaaccatcc gtgctgccgc tgcggaacgg cgtcagctgg
     6601 ctatccgtgg cagcggcagc aagacgtttt acaccggcgt ggcccagggt gaaatccttt
     6661 atacacgggg gctgcggggc nnnnnnnnnn tcgtcagcta ccagccgagt gagctggtgc
     6721 tcactgcgag aaccggaacg tcgctggtcg aaattgaggc ggcactgacg gaacaaggcc
     6781 agatgctggc ctgcgagccg ccgcatttcg gcgacggcgc caccctgggt ggtgtggtgg
     6841 cgtgcggatt ttccggcccg cgccggccgt ggggcggtgc cgtgcgcgac ttcgtgctgg
     6901 gggtgcgcat ggttaatggc cagggcgaag tgctgcattt cggcgggcag gtgatgaaga
     6961 atgtcgccgg cttcgacgta tcgcgcctga tggcgggctc gctcggcagt ctcggcctgc
     7021 tgctggaggt ttcgctcaag gtgttgccgc gtccggccta cgaaatcacg ctgcggcgcg
     7081 agtgcacgca agccggggcg ctggcgcaat gcgcggcctg ggcggcacgc ccgctgccgc
     7141 tttccgctgc tgcctggctg aacggggcgc tgtatgtgcg tctttccgcc agcgaggccg
     7201 cggtacgggc ggcggcgcag gccacgggcg gcgaggcggt gccggacggc acggcattct
     7261 ggcagtcgct gcgcgagcac gctcagccgt tctttacgct ggccgagggc gaaaccctgt
     7321 ggcggctgtc gctgccgccg gccgccccgg tgtccgatct ggccggccgt tggtgcgacg
     7381 actggggtgg ggcgcagcgt tggctggtga cggcacagga cgccgaggct gtacgccagg
     7441 ccgcgtgcgg cgcgggcggc cacgccgtgc cgtttcgtgg cgcacaggcc gggcaactcc
     7501 agctcgcgcc gggtttggca gcactgcatg accggctcaa ggcggccttc gatccgcatg
     7561 gcgttttcag cccggtggcc cgctgatgca gacccggttg ccgccagtat tgcagcaaac
     7621 tgctgaaggg cgcgaggccg aggccatcct gcgcgcttgc gtgcactgcg gcttctgcac
     7681 cgcggtctgc cccacctacc gcctgctggg caacgaactg gacagcccgc gcggccgtat
     7741 ttaccttatc aagagcctgc tggaaggcga ggccataggc gaacagacgc gcacccacct
     7801 cgaccgctgc ctgacctgcc atgcctgcga aaccgcctgc ccgtccggcg tgcaatacgc
     7861 gcggctggca gacatcggcc gcgccgcgct ggaacggcag gtgccgcgcc cctggccgca
     7921 gcgcctgtgg cgctggctgc tgcgccgcac gctggcttcg ccgcgcgtgt ttgctggcct
     7981 gctggcaatt ggccggactg caaggccgtt actgcctgcg ccactcaaac gccgcattgc
     8041 gccagccacg gtcgctggga gctggccgcc gccgcgccat gcacgtcgca ttctggctcc
     8101 tgccggctgc gtgcaacccg ccctggctcc tggcatcgat gctgccatgg cgcggctgct
     8161 ggatgcgcac ggcgtctcgc tggtcaaggt cgataccggc tgctgcggtg cgctggacca
     8221 gcacttggcc gcaccggagc aggcattgca ccgtgcccgc gtctacgtcg acgtgctgtg
     8281 gccgcagatc gaagccggtg cagaagcggt ggcggtcagc gccaccggct gcggcgcgat
     8341 ggtgcgcgaa tatgcccatt tgctgcggga tgattctgcc tatgccgcca gggcggcacg
     8401 cgtttcggcg ctgtgccgcg acccggtgga acttctggaa accttgcccg tggctgatgt
     8461 tgggcgcggt cgccgcattg ccttccactc gccctgcaca ctgcagcatg ccctgcggct
     8521 ggaaggccgc gtcgaggcgc tgctgcagcg cgccggtttc atcctgctgc cggtagaaga
     8581 ggctgcgcaa tgctgtggcg cggcaggcac ttactccatc ctgcaaccgg ggttgtctga
     8641 gcagatgttg caacgcaagc tcgggtatct gcaacagcac gcgccggaat tgatcgccac
     8701 cgccaatatt ggctgcctca cccatttgca gggcggcggt gagatgagcg tggtgcattg
     8761 ggtggaattg ctggccgagg gtgattgata acaaagagct ggcagttgca atcgaccatt
     8821 gggtcaactg gcgcacggaa gccagccaga ctcgagccgc cggccgcggc tattgctata
     8881 attaaccgag ggtattaccc tgcgagtttc tgcttacatg acattccggg ggagggacac
     8941 tgcatgctgt gggtactgtt ttacgcgact gccgttatca cactgatcct gtacttcacc
     9001 ggcttcctca cacgccataa tctggaatgg ctggtgctgg tgttggccgc ctctgttttt
     9061 cctgcagtca tctacctcta gttatcgcaa gctttcaggc aaaaaaaggc ggccttgcga
     9121 ccgcccaggg aggagatttt cggttggagt tttgttagtt gatggtcagc ttcttgctcg
     9181 atgctaccgt tttcttgggc agtgccagtt ccagcacgcc gtcggcgtat ctggcttgtg
     9241 atgcagcttc gtcgatgtcg ctggcgacgg agaagctacg tgcgaccttg ccgtagtagc
     9301 gttccgagcg caggactttt tcgccttcct tcacttcctt ttcctgtttg gcttcggcgc
     9361 tgatcgacac ctggttgccg tcgatggtga cgtggatgtc ttccttcttc acgccgggga
     9421 tttcggcgtg cacggtgtag cccttgtcgt cttccttcac gtccatcttg atttgtatgt
     9481 ctggcgcgcc ttcaatgcgt accgggcgga aaaagccctt aaatacatca tcgaacggat
     9541 ccaatgcgga aatctggaac gggtcgaagc gggttacgtt agccatttga tgtctccttt
     9601 cgtcgtgaat tgaaaaatca agccagttcg gccgttaatt attagatggg gagtgtttaa
     9661 cggatttcaa gggcttgaaa agacagaata tccgtacacg ggcataaaaa aagcggcctt
     9721 gcgaccgctc agggaggaga tgttcgtgga accactcaac ttgaagcaag tataacgaat
     9781 cagtaaataa aggacaagta tatcttgatt aaaaaatacg ctgtcagagc acgtaacgcg
     9841 ccagatcctc gtcctgtgcc aggccttgca atttggcatt gacgtacccg gcatcgatag
     9901 cgagcatgcc gtcggcatgc ttgtcggcat caaacgagac ttcctccagt agtttttcca
     9961 tcaccgtatg taggcggcgc gcaccgatgt tttcggtatg ttcgttgact tgccaggcga
    10021 tctcggccag acggcgtacc gcatccggct tgaacgccag cgtcacgttc tcggtttcca
    10081 gcagtgcctg gtactgtcgc gtgaggcagg catcagtgcc ggtgaggatt tgctcgaagt
    10141 cgccgaccga gagcgaatcc agttccacgc ggatgggcag gcggccttgc agttccggga
    10201 tcagatcgga cggcttggcg agatggaacg cgccgctggc gatgaacaga atgtggtcgg
    10261 tcctgaccat gccgtacttg gtggtgaccg tggtaccttc caccagcggc agcaggtcgc
    10321 gctgcacgcc ctggcgggag acctcggcgc cactgctttc gctgcggctg gtgatcttgt
    10381 cgatctcgtc gagaaatacg atgccattct gttctacatt gtgcatggca tgcagcttga
    10441 tgtcgtcctc gttgaccagc ttggcggcct cctcgtcggt gatgtgtttc atggcatcgg
    10501 cgatcttcag cttacgcagc cggggcttgc cgccgcccat gccctggaat gcgttctgga
    10561 tctggctggt cagttcctcc atgcctggcg gagagaagat ttccatgtga gcggtgggcg
    10621 cggccagctc gatgtcgatt tccttggcat ccagcttgcc ttcgcgcagc atcttgcgga
    10681 atttctgacg cgtttctgaa tcggcgtgcg cggactcctc gttaaggctg cgggccggcg
    10741 gcagcagaat atcaaggatg cgatcctcag cggcatcggc ggcccggatg cgcacctttt
    10801 ccatctcctg ctcgcgcgtc tgctttaccg ctatttcggc caggtcgcgg atgatggaat
    10861 ccacgtcctt gccgacatag ccgacctcgg tgaacttggt ggcttccacc ttgatgaatg
    10921 gcgcattggc cagccttgcg aggcgccggg cgatctcggt cttgccgacg ccggtggggc
    10981 ctatcatcag gatgttcttg ggtttgatct cctgccgcat cggctcggcc acctgggcgc
    11041 ggcgccagcg attgcgcagt gcaatggcga ccgcgcgctt ggcggcagcc tggccgacga
    11101 catgtttatc caattcgtgg acgatttcct tgggggtcat ttgcgacatc gtatttattc
    11161 gagcgtttcg atgatgtggt tctggttggt gtaaatgcac agatcggcgg cgatggtcag
    11221 cgcttttttc acgatctcgg ctgacggcag atcggtatgg tcgagcaggg cgcgtgcggc
    11281 agattgcgca taagccccgc cgctaccgat ggcgagtagg ccatattccg gttccagcac
    11341 gtcgccgttg ccggtgatga tgagcgagga ctccttgtcc gctactgtca acatggcttc
    11401 cagacgtcgc agcatgcggt cggtgcgcca gtccttggcc agttctaccg cgctgcgtag
    11461 cagattgccc tgatgttttt ccagcttggc ttcgaagcgc tcgaacaggg taaaggcatc
    11521 tgccgtgccg ccggcgaatc cagccagaat gttgccgtga tagaggcgcc gcaccttgcg
    11581 cgccgtggcc ttgaccacga tgttgccgag cgtgacctga ccgtcgccgc ccatggcgac
    11641 cttgtcgccg cgccgcacgg agaggatggt ggtgccgtgg tattgttcca tgggaattcc
    11701 ttgcagtttc aaactagatt agggtggcgg ccgggatttc aagggctaag gcaaaatcag
    11761 ccaatgcgtt cgttgcaata gttgattata aaactcaact ataatgcaag tatctccagc
    11821 catccgtgtc attgccatgc ccaaccgtct tgccagcgaa accagtccct acctgcgcca
    11881 gcacgccgac aacccggttg actggtatcc ttggggtgag gaagcgctgg cgttggcgcg
    11941 gcagcaggac aagcccatcc tgctgtcgat cggttattca gcctgtcatt ggtgccatgt
    12001 catggcgcac gaatcgttcg aggatccggc caccgcggcg ctgatgaacg agctgttcgt
    12061 caatatcaag gtggaccgcg aagagcggcc agacctcgat cagatctacc agagcgcgca
    12121 tgccatcctg tcgcgacgcg gcggtggctg gccgctgacc atggttctta ctccagagca
    12181 ggtgccgttc ttcggtggca cctatttccc gccggcgcca cgctacggca tgccgggctt
    12241 ttccgacctg ctgcgacaaa tagcgatggc ctggcgcgag cgtcgggctg atatcgaagc
    12301 gcagaatgcc gaagtgctga gtgtgctggt gaaaatggta cggcaggacg caccggcggc
    12361 cctggatggc gcggtggtga ctgctgccgt gtcgcagctg gcgcaaatgt tcgatagtgt
    12421 gaatggtggc ttcggcggcg cacccaagtt ccccaacccg gccgacctgg ctttgctgtt
    12481 gcggcgcgcc agtgcaggcg acccgcaggc gtgccacatg gtgaccctga cattgtccaa
    12541 aatggccgac ggcggcatct acgaccacct gggtggcggc ttttgccgct acagcgtgga
    12601 cgaccactgg gaaatcccgc acttcgagaa aatgctgtac gacaacgggc agttgctcag
    12661 cctgtacagc gaagcctggc tgatgaccgg tgacaaccaa taccgggaag tcgtggggca
    12721 caccgtggac tggctgctgc gcgagatgcg cgcgccggac ggcgctttct attcctcgct
    12781 ggacgccgat agcgaggggg aggaaggcag gttctacgcc tggcagcgcg acgaggtgcg
    12841 tcgactgctt gacgcgccgg agtatgcctt gctcgagaag cgctatggct tggacagcac
    12901 gcccaacttc gagcatactt ggcacctgca tgtgactagc gactcggaaa ccgctgcgcg
    12961 gcagctggga ttgacgccgc aacagtcggc gaatctatgg caaagcgcgc gcgccaagct
    13021 gtggcgggtg cgcgagctgc gggtacgccc ggggcgagac gacaagatac tgaccagctg
    13081 gaatgcgctg gtcatcaccg ggatggcgcg tgccggtcgc gcctttggtc gccctgactg
    13141 gattctggcc gctcagcaag cggttgattt catccatacg cagatgtggc gcgagggcca
    13201 gttgctggcg acctgcaagg acggcagggc gcatctcaac gcctacctgg acgaccacgc
    13261 tttcctgctg cacgcgctgc tggaactgat gcaggccgaa ttccgcagaa ccgacctcga
    13321 ctttgccgtg gagatagccg aagctctgtt ggcgcgtttt caggacgatg aacacggcgg
    13381 tttcttcttt accagccacg accatgaagc cctgatccaa cggccaaaat ccgtacacga
    13441 taatgcgacg ccgtctggca acggcgttgc cgcctacgcc ttgcagcggc tggggcatgt
    13501 ggtgggcgag gcgcgctatc tggccgccgc cgagaagacg ctgcgcgcct tcgccggcac
    13561 tatgcagcgc aacccggccg ctagtccgag ccttgtggct gcgtttgacg aatacctggc
    13621 gccccccgcc ttgctatatc tgagggggcc gcaggaaaag ctat
//
LOCUS       DAOMFA010000026        13482 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_408_length_13482_cov_5.594027, whole genome shotgun sequence.
ACCESSION   DAOMFA010000026 DAOMFA010000000
VERSION     DAOMFA010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 13482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13482
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_408_length_13482_cov_5.594027"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(51..1223)
                     /locus_tag="PKW44_02475"
     CDS             complement(51..1223)
                     /locus_tag="PKW44_02475"
                     /EC_number="4.2.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA];
                     GO_process: GO:0071268 - homocysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-succinylhomoserine sulfhydrylase"
                     /protein_id="HQR50487.1"
                     /translation="MDNSLHLETLGVRAGSAHTEFGENSEPIFLTSSFVFDNAAQAAA
                     RFGGQEPGNIYSRFTNPTVTMFQAKLAALEGAEFCVATSSGMAAILACVMGLCSAGDH
                     VVASRSVFGTTVQLFGNILQRWGLNVTFVSLTDLKEWEAAVKPNTKLFFVETPSNPLT
                     EICDIGALAALAHKSGAFLAVDNCFCSPALQRPIDLGADIVIHSATKYIDGQGRCLGG
                     AVLGKKDTLEPVYGFLRTAGPTMSAFNAWVFLKGLETLSLRMEAHSRNALELALWLEQ
                     QPQVERVYYPGLPSHPQYELAKHQQKTGGGIVSFDVKGGKDAAWRLIDSTRLLSITAN
                     LGDAKTTITHPATTTHSRVTPEARVAAGIGDGLVRIAVGLEHVEDIKTDLVAGLKT"
     gene            complement(1398..2939)
                     /gene="purF"
                     /locus_tag="PKW44_02480"
     CDS             complement(1398..2939)
                     /gene="purF"
                     /locus_tag="PKW44_02480"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HQR50488.1"
                     /translation="MCGIIGIVGKNPVNQMLYDGLLVLQHRGQDAAGIVTSDGGTFYM
                     HKNNGLVQDVFQTRHMRSLLGNAGIAHVRYPTAGSSSAAEAQPFYVNSPFGIVLGHNG
                     NLTNSEQLKSEMFKQDLRHINTNSDSEVLLNVLAHEIENSARSTALDVDMIFDAVAGV
                     HKRVKGAYAVVAMIANYGVLAFRDPHGIRPLVIGKAETDKGVEYAVASESVALDVLGF
                     QLVRDVVPGEAIFIDMDGNFYSRQCAENPVLNPCIFEYVYLARPDSVIDNVSVYQTRL
                     HMGESLADKIHREWRSHDIDVVIPIPDTSRPSALQLANALNLTYREGFIKNRYIGRTF
                     IMPGQALRKKSVRQKLNPIGMEFKGKNVLLVDDSIVRGTTSQRIVQMAREAGANKVFF
                     ASAAPPVRFPNVYGIDMPTRDELLATGRNDEQICREIGADALVYQDLEALVHGVQLSN
                     PELKQFDCSCFDGHYVTGDITEAYLSAIESARGDGAKLRKPINSSTQLDLNLVGSEEQ
                     EEEAV"
     gene            complement(2956..3444)
                     /locus_tag="PKW44_02485"
     CDS             complement(2956..3444)
                     /locus_tag="PKW44_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HQR50489.1"
                     /translation="MTVFDYAVLAIIGVSVLLSLMRGLVREMLGLTGWVAAFFVARFY
                     AVEVAPLLPAAIPNEPLRLVAAFAMLFLTTLLAVSLVTILLGELLKQVGLGVLDRGLG
                     AVFGLARGLLIVAVLVLLGGMTSLPQDARWRNAMFSAPLEAMVLACKPWLPEEMTKYL
                     KY"
     gene            complement(3472..4065)
                     /locus_tag="PKW44_02490"
     CDS             complement(3472..4065)
                     /locus_tag="PKW44_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HQR50490.1"
                     /translation="MVVDRSSEQEIQFKKRARRRLVGAIALVLLMVTVLPVILDNHDD
                     QTQQPEIAISIPSQNDTDFTSKVVPGEVAGSPAPVEAKNAEVRPAQKNDSETAMPAKP
                     AVSQTPSKSTETNPAIVPANAAKSSYLVQIGLFSDESRVKQLQGKIKELGIETSTLRD
                     GQKIRLRAGPYGSKEEAIQVSDKLKAAGYQTMLVKSN"
     gene            complement(4055..5338)
                     /gene="folC"
                     /locus_tag="PKW44_02495"
     CDS             complement(4055..5338)
                     /gene="folC"
                     /locus_tag="PKW44_02495"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HQR50491.1"
                     /translation="MPADLSGWLIYLEQLHPKSIALGLDRVGQVRDRLALHPEFPVIT
                     VGGTNGKGSTCAMLERIYLEAGYRVACYTSPHLLRYNERVRVDGVEASDSQLCAAFAA
                     VEEARGETPLTYFEFGTLAAMWLFIQGDAEVAILEVGLGGRLDAVNIFDPACAIVTSV
                     DLDHLDYLGSTRASIGREKAGIYRKGVPALCGEPQPPATVEDCAKKTGANFRQIGVDF
                     GYVVRGGVWDFVSSGTSLQNLPMPALHGSFQLGNATCAIEAVHSLQRQLPVALEAICA
                     GLMHVTLQGRFQVFCGRPHVILDVAHNPHAAKGLAENLRIHPHAGRTLAVFAMLGDKD
                     IAGVVSALAGEINAWFVAPIRHSRGADADTLCACLREVVPEVQVHRFTDVEAAFHQAC
                     LSAGENDRIAAFGSFYTVADVMRDLPAMGEEIHGG"
     gene            complement(5352..6218)
                     /gene="accD"
                     /locus_tag="PKW44_02500"
     CDS             complement(5352..6218)
                     /gene="accD"
                     /locus_tag="PKW44_02500"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019898780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HQR50492.1"
                     /translation="MSWLQKLLPPKIKRSIGASKKTVPEGLWSKCPCCEAVLYRTDLE
                     NNQEVCPKCGYHNRISARTRLDFILDAEGRYEIGVEVTPLDPLKFKDSKRYPDRLKAS
                     QAETGETDALVVMQGAIKTVPAVVAAFEFKFQGGSMGSVVGERFVRGVSAAIEQKVPF
                     ICISASGGARMQEGLFSLMQMAKTSAALTQLSQAKLPFISVLTDPTMGGVSASFAMLG
                     DIIIAEPQALIGFAGPRVIEQTVRETLPDGFQRAEFLLEHGAIDLIVDRREMRDKFAT
                     LIGNLMRHPAVA"
     gene            complement(6234..7031)
                     /gene="trpA"
                     /locus_tag="PKW44_02505"
     CDS             complement(6234..7031)
                     /gene="trpA"
                     /locus_tag="PKW44_02505"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HQR50493.1"
                     /translation="MSRIQSTFEILKKQGRKALIPYITAGDPHPGLTVELMHRLVASG
                     ADIIELGVPFSDPMADGPVIQRASERALAHHVRLRDVLGMVAEFRQQDQSTPVVLMGY
                     ANPVEAMGYEKFASSAKESGVDGVLTVDYPPEESEAFVAKLKENGIDPIFLLSPTTEA
                     ARVDAIVRLASGFIYYVSLKGVTGAGHLNVADVAHKVAAIRQHTELPVGVGFGVRDAA
                     TAKAIAAIGDAVVVGSRMVQEIENSSTENVAGNLASLTAELRGAIDA"
     gene            complement(7031..8233)
                     /gene="trpB"
                     /locus_tag="PKW44_02510"
     CDS             complement(7031..8233)
                     /gene="trpB"
                     /locus_tag="PKW44_02510"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HQR50494.1"
                     /translation="MQVYDFPDARGHFGPYGGIFVAETLIEALEELRAMYERYRHDPE
                     FLAEFEHDLKHFVGRPSPIYHAKRLSDHFGGAQIYLKREDLNHTGAHKVNNCIGQALL
                     AKRMGKPRIIAETGAGQHGVATATVAARMGLECVVYMGSEDVKRQAPNVFRMKLLGAT
                     VVPVESGSKTLKDALNEAMRDWVTNISNTFYIIGTVAGPHPYPMMVRDFQSIIGIEAK
                     QQMPELTGRQPDAVIACVGGGSNAMGIFYPYIEEENVRLIGVEAAGLGMETGKHAAPL
                     TANSPVGVLHGNRTYLMQDEDGQIIETHSISAGLDYPGVGPEHAWLKDLHRAEYVAVT
                     DDEALQAFHTLCRMEGIIPALESSHALAYAAKLAPTMARDQILLVNLSGRGDKDINTV
                     ANLSGIKL"
     gene            complement(8220..8843)
                     /locus_tag="PKW44_02515"
     CDS             complement(8220..8843)
                     /locus_tag="PKW44_02515"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HQR50495.1"
                     /translation="MRTRTKICGITRVEDADAAVAAGADAIGLVFYANSPRHVGVDRA
                     AEIVASLPPFVAAVGLFVDAGPAKVRAVLAKVRLDLLQFHGDESPEYCQSFNLPYIKA
                     VRVRPELNLVQYAADFASAGALLLDAWQEGVAGGTGQVFDWDLIPSNLPKPVILAGGL
                     NANNVGAAIRRVRPYAVDVSGGVEREKGIKDADKIAAFMRGVLDAGV"
     gene            complement(8855..9400)
                     /locus_tag="PKW44_02520"
     CDS             complement(8855..9400)
                     /locus_tag="PKW44_02520"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019898696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine-guanine phosphoribosyltransferase"
                     /protein_id="HQR50496.1"
                     /translation="MSSSISPEQLLQNAEMIYESRQVDAAVHRLAQEITEKLGDSAPL
                     VLCVMGGAVVFSGWLLPRLQFPLEFDYVQASRYHNGTSGQHLIWKVTPGENVRGRTVL
                     LLDDILDEGHTLAAVRQKCLEAGASRVAIAVLTTKETGRPKPVQADFVGLTVPNCYVF
                     GCGMDVHGWWRNLPGIYALKD"
     gene            complement(9402..10271)
                     /gene="galU"
                     /locus_tag="PKW44_02525"
     CDS             complement(9402..10271)
                     /gene="galU"
                     /locus_tag="PKW44_02525"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HQR50497.1"
                     /translation="MNKITKAVFPVAGMGTRFLPATKASPKEMLPVVDKPLIQYAVEE
                     AIAAGVTELIFITGRNKRAIEDHFDKAYELEAELESRGRQKMLEQLRGILPSHVSCIY
                     IRQAEALGLGHAVLCARPVVGDEPFAVILADDLIDGNPPVLKQMTDLYAQYGKSLLGV
                     ETVARSETSGYGIVDAHGIAASLLEVRGIVEKPQPQEAPSTLAVVGRYILNPRIFDCL
                     EALSPGKGGEIQLTDGIAALMAQEQVLAYPFQGRRYDCGSKLGYMQANVEFAMRHPEI
                     GRKFADYLRTVPR"
     gene            complement(10282..12162)
                     /locus_tag="PKW44_02530"
     CDS             complement(10282..12162)
                     /locus_tag="PKW44_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HQR50498.1"
                     /translation="MKIDRLPRAFLVFVHDVFAAAMAWVLAYLLRFNFSLPDYADSAI
                     LHTLPWVLLLQSAIFLKFGLYRGIWRFASIPDLKRILTAVGVAALAVAALLPFLRQSY
                     VVPRSVLLLDPILLLLMMGGSRFLYRAWKEHHLYGPRQLQGEPVLVMGAGNTAVSLLK
                     EMERSRDWRVVGLLDDSSGMHGRHIYGVKVLGSLSEAPIYAARMGISHVILAMPSVSH
                     SVRRRAIDIAEQAGLAVLTVPSFDDLASGKVSVSQVRRVEVEDLLGRDPVQLDDAGLH
                     SLISNRVVLVTGAGGSIGSELCRQISRFNPETLVLFELNEFALYQLEQEFRENFPRQH
                     IACVVGDVRNAERVAGVLRRFRPQVVFHAAAYKHVPLMEWENVSEALANNVLGTHAVA
                     AACKQEGVGKMVLVSTDKAVNPTNVMGASKRLAEMACQGLQEEGGTRFVMVRFGNVLG
                     SSGSVIPKFREQIARGGPVTVTHPEVTRYFMSIPEAAQLVLQAGLMGDGGEIFVLDMG
                     EPIRIAHLAQEMIRLSGFGEDDIKIVYTGLRPGEKLYEELLADDEHTLPTPHPKLRIA
                     RARGANSDWVAALLQWVESAARMEEPVVKQELKTWVEEYTGDIHSVSPDSIMVPVSTT
                     VH"
     gene            complement(12159..13160)
                     /locus_tag="PKW44_02535"
     CDS             complement(12159..13160)
                     /locus_tag="PKW44_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013031002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HQR50499.1"
                     /translation="MSKNFILAAGGSFVVALSVLLLLVNGRIARTVLDQPNERSLHTV
                     AVPRTGGLAIMTGIVAAWGMAWHPELLILTCCMLFLVALSFLDDIYSLSAGWRFAAHS
                     LAAAVFLGLTMLSAPDPWWWPVLLVAMVWMTNLYNFMDGSDGLAGGMALFGFSFYALG
                     AWQAADAGLAFSSLAVAAASAAFLLFNFHPARIFMGDAGSVPLGFLAAALGLSGWQQG
                     DWPLWFPLLVFSPFIADATVTLVKRMLRGEKVWQAHRSHYYQRLVQMGWGHRSTALAE
                     YGLMALAGGSALCLASQAWPLQLAGLLLWGAIYCGLAIAVDRRWFKLHLPKIHVANQ"
     gene            complement(13189..>13482)
                     /locus_tag="PKW44_02540"
     CDS             complement(13189..>13482)
                     /locus_tag="PKW44_02540"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004176058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HQR50500.1"
                     /translation="GEDAHTASLFPGKAWESQAMAPAIAVHGAPKPPPERVSLSASRL
                     SRAHRVIFFVTGAGKRDAVKLWRSGADLPVAAVAPAAGVEVYMDVAAEPALTD"
BASE COUNT         2846 a         4206 c         3751 g         2679 t
ORIGIN      
        1 actcaaacgg tgcctttcgt ccccgttttc atttgtatcg cggcgtacag ctatgtttta
       61 aggccagcca ccagatcggt ctttatatcc tcaacatgct ccaaccccac tgcgatgcgc
      121 accagaccgt cgccgatacc cgcggccacc ctcgcctccg gcgtcacgcg gctgtgcgtt
      181 gtggtcgccg gatgcgtgat ggtggtcttg gcatcgccca ggttagcggt gatggacagc
      241 agacgggtcg agtcgatcag ccgccatgcc gcatccttcc cacctttgac gtcgaacgac
      301 acgatgccgc cgccggtctt ctgctggtgc tttgcaagtt catattgcgg atgtgatggc
      361 agcccagggt aatagacccg ttccacctgc ggctgttgtt ccagccacag cgcaagttcc
      421 agcgcgttgc gggaatgcgc ctccatacgc aggctgagcg tttccagccc ttttaggaac
      481 acccaggcat taaacgcgct catggtcggc ccagcggtgc gcaggaagcc gtaaaccggt
      541 tccagcgtat ccttttttcc cagcaccgcc ccgcccaggc aacgcccctg cccgtcgata
      601 tacttggtag cggagtgaat gacgatatca gcgcctaggt ctatcggtcg ttgcaatgcc
      661 ggcgagcaaa agcaattatc tacagccaga aaagccccgg atttatgtgc cagcgccgcc
      721 aatgcgccaa tgtcacagat ttcggtcagt ggattggacg gcgtttccac gaaaaacagc
      781 ttggtgttgg gcttgactgc cgcttcccat tccttgaggt cggtcagcga aacgaaggtc
      841 acgttcaatc cccaacgctg caggatgttg ccgaataatt gcaccgtagt gccgaacaca
      901 ctgcgcgaag ccaccacatg atcaccggcg gaacacaagc ccatcacgca agccaggatg
      961 gcggccatgc cgctcgatgt cgccacgcaa aattccgccc cctccagcgc cgccaacttg
     1021 gcctggaaca tggtgacggt gggattggta aaacgcgaat agatgtttcc cggctcctgc
     1081 ccgccaaaac gcgctgccgc ttgggccgca ttgtcgaaca cgaaactgga agtaaggaaa
     1141 atcggctcgg agttctcgcc aaattcggta tgtgcgctgc cggcccgcac acccagcgtt
     1201 tccagatgca atgagttgtc catgctgtta ttcacctttt ccgcctgcga gcaggcataa
     1261 aaaaacccat ttcagcattg gctaaaacgg gctttctcgg ctcgctttag ctgcatttat
     1321 taagcgcccg caagctgaaa ctctcaaatc ggcgcagtaa aacaataatt cctggcaacg
     1381 cttttgtcaa accggcgtca aactgcctct tcttcctgct cttccgatcc taccagatta
     1441 aggtcaagct gcgtgctgga gttgatgggc tttctcaact tggcgccatc gccacgagcg
     1501 gactcgatgg ctgaaagata ggcctcggta atatcgccgg tcacatagtg gccgtcgaag
     1561 caggagcagt cgaactgctt gagctcagga ttgcttagct gcacaccatg caccaacgct
     1621 tccaaatcct ggtagaccag cgcatcggcg ccgatctctc ggcatatctg ctcgtcattg
     1681 cggccggtag cgagcagttc atcacgtgtc ggcatgtcga taccatagac attgggaaag
     1741 cgcaccggcg gcgcggcaga agcaaaaaac accttgttgg cgccggcttc gcgcgccatc
     1801 tgcacgatgc gctgtgaagt ggtgccgcgc acgatggaat cgtccaccag cagcacattc
     1861 ttgcccttga actccatgcc gatcgggttg agcttctggc gtacggactt cttgcgtaac
     1921 gcctgccctg gcatgatgaa ggtccggccg atgtagcggt tcttgatgaa accttcgcga
     1981 taggtcagat tgagcgcgtt ggccagctgt agcgcgctgg ggcggctagt gtctggaatg
     2041 ggaatgacca catcgatatc gtgagaccgc cactctcggt ggattttatc tgccaggctc
     2101 tcgcccatgt gcagacgggt ctggtacacc gacacattgt cgatcaccga atcagggcgc
     2161 gccaagtaga cgtattcaaa aatacaaggg ttgagcaccg ggttctcggc acactggcgc
     2221 gagtagaaat tgccgtccat atcgataaaa atggcctcgc cgggcacgac gtcgcgcacc
     2281 agttggaaac ccagcacatc gagcgccacg ctctccgaag caacagcgta ttccacgccc
     2341 ttgtccgttt cagccttgcc gatcaccagc ggacggatac cgtgtgggtc tcggaacgcc
     2401 agcacaccat agttggcgat catcgccacc acggcgtaag cgcccttcac ccgcttatgc
     2461 acgccggcca ccgcatcgaa aatcatgtcc acgtcgagcg cggtgctacg cgcgctgttt
     2521 tcgatctcgt gcgccagcac gttgagcagc acttcggaat cggaattggt gttgatgtga
     2581 cgcaggtcct gcttgaacat ctcgctcttg agctgttctg aattggtcag attaccgtta
     2641 tgccctagta cgatgccgaa cggcgaattg acgtagaacg gctgggcctc ggcggcggaa
     2701 gacgagccgg cggtcggata gcgcacatgg gcgataccgg cattgccgag caggctacgc
     2761 atgtggcggg tctggaacac gtcctgcacc aggccgttgt tcttgtgcat gtagaaagtg
     2821 ccaccatcgc tggtgacgat gcccgcggca tcttggccac gatgctgcag caccagcaac
     2881 ccgtcgtaca acatctggtt gacgggattc ttgccgacga tacctatgat tccgcacata
     2941 acagggtagc ctcgattagt atttcaaata cttggtcatt tcttcaggca gccatggttt
     3001 gcatgccagc accatggcct ccaacggtgc actgaacatc gcattgcgcc agcgcgcatc
     3061 ttgcggcaag gaagtcattc cgcccagcag caccagcact gcgacaatca acaacccgcg
     3121 cgcaaggccg aacacggcac caagcccccg gtccagcaca cctagtccaa cctgtttaag
     3181 cagttcgccc agcaaaatcg tgaccagact gacggccagc aacgttgtca gaaaaagcat
     3241 tgcaaaagcg gccaccaggc gcaggggctc gttaggtatg gctgcaggca acagcggcgc
     3301 cacttccacc gcgtaaaacc tagcaacgaa aaacgcggcc acccagcccg tcaaccccag
     3361 catttcccgc accagaccac gcattagact caacagtacg gatacgccga tgatggcaag
     3421 taccgcgtag tcgaaaaccg tcatagtgtg ctcgatgcaa ttgcctgatt gtcagttgct
     3481 tttgaccagc atggtctgat agccggctgc cttgagttta tcgctcacct gtatggcctc
     3541 ctctttgctg ccgtatggtc cggcacgcaa acgaattttc tggccatccc tgagggtgct
     3601 ggtctcgatg cccaattcct tgatctttcc ctgcaactgc ttgactctcg attcatcgga
     3661 gaacagaccg atctgcacca ggtagctgct tttggccgcg tttgcgggaa cgatagcagg
     3721 gttggtttca gtggattttg atggcgtctg ggacacagcc ggcttggcag gcatagccgt
     3781 ttccgagtca ttcttctgtg caggtctaac ttccgcattt ttcgcctcta ccggtgccgg
     3841 actgccggcc acctcccccg ggacgacctt ggaggtgaag tctgtgtcgt tctggctggg
     3901 gatggagatc gcgatctctg gctgttgggt ttgatcgtca tgattgtcga gaatgactgg
     3961 cagcaccgtg accatcagca gtaccagagc aatagcacct actaaacgac gccgcgcgcg
     4021 ctttttgaac tggatttcct gttcactcga ccgatcaacc accatgaatt tcctccccca
     4081 ttgccggcag atcgcgcatc acatccgcca ctgtgtagaa tgaaccgaag gccgcaattc
     4141 tatcattttc gcccgcactc aaacaggctt gatggaaagc tgcctcgaca tcggtaaagc
     4201 gatgcacttg cacttccggc actacttctc gcaggcaggc acaaagcgta tcagcgtcag
     4261 ccccacgcga atgccgaatc ggtgccacga accaggcatt gatctcgccc gctagcgcgg
     4321 aaaccacccc tgcaatatcc ttgtcgccca gcatggcgaa taccgctagc gtacgcccgg
     4381 catggggatg aatgcgcagg ttttccgcca acccttttgc cgcatgcgga ttatgcgcca
     4441 catccagaat tacatgcggc ctgccacaga acacctggaa ccgtccctgc aacgttacat
     4501 gcatcaaccc ggcgcaaatc gcctccaatg cgacaggcag ttgccgttgc aggctatgta
     4561 ctgcctcaat ggcgcacgtg gcgttaccga gctggaaact gccatgcagg gctggcatcg
     4621 gcaagttctg caggcttgtg ccggaggaaa cgaagtccca gacgccacct cgcacaacat
     4681 agccaaagtc caccccgatt tgcctgaaat ttgcaccagt ttttttcgcg caatcttcta
     4741 ctgtagccgg tggctgtggc tcgccgcaca atgcaggcac ccctttgcga taaatgccag
     4801 ccttttccct cccgatgctt gcgcgcgtgc tgccaagata atccaaatga tcgagatcga
     4861 cgctggttac gatggcgcaa gccggatcaa agatattgac cgcgtccaag cgtccaccaa
     4921 gaccgacctc cagaattgcc acttctgcgt cgccctgaat gaataaccac atcgccgcca
     4981 gcgtgccgaa ctcaaaatac gtaagcggcg tttctcctct cgcctcttca actgcggcaa
     5041 acgcggcgca tagctggctg tccgatgcct ccactccgtc aacacgcacc cgctcgttgt
     5101 aacgcaacag atggggagag gtgtagcagg caacccggta gcccgcctcg aggtagatgc
     5161 gttccagcat ggcacaggta gatcccttgc cattggtgcc accgacggta atgaccggga
     5221 attccggatg aagggccagc cggtcccgta cctgcccgac gcgatcgagg ccgagcgcga
     5281 tggacttggg gtgcagctgt tcgaggtaaa tcagccagcc gctgagatcg gctggcatcg
     5341 tggatgcagg tttaggcaac ggcgggatgg cgcatcaggt tgccgatcag ggtggcgaac
     5401 ttgtcgcgca tctcgcggcg gtcgacaatc aggtcgatcg cgccatgctc cagcaaaaac
     5461 tcggcgcgct ggaagccatc cggcagggtt tcgcgcaccg tctgctcgat cacgcgcggg
     5521 ccggcaaaac cgatcagcgc ctgcggctcg gcgatgatga tatcgcccag catggcaaag
     5581 ctggccgaaa ccccacccat ggtggggtcg gtcagtaccg aaatgaacgg caattttgcc
     5641 tgactcagtt gtgtcaatgc ggcactggtc ttggccattt gcatcagcga gaacaatccc
     5701 tcctgcatgc gcgcgccacc actggcggaa atgcagatga atggtacctt ctgttcgatg
     5761 gcggctgaca ccccacgcac gaagcgttcg cctaccaccg aacccatgga accaccctgg
     5821 aacttgaact cgaacgcagc gaccaccgcc ggcacggtct tgatcgcccc ttgcatcacc
     5881 accagcgcat cggtctcgcc ggtttccgcc tggctggcct tgagtcgatc cggatagcgc
     5941 ttgctgtcct tgaacttgag ggggtccaga ggagtcacct cgactccgat ttcataacgc
     6001 ccctcggcgt ccagaatgaa gtccagccgg gtgcgggcgg aaatgcggtt gtggtagccg
     6061 cacttgggac aaacttcctg attgttttcc aggtcggtgc ggtataacac cgcctcgcaa
     6121 caaggacact tgctccacag accttccggc actgttttct tgctggcacc tatactgcgc
     6181 ttgattttcg gggggagaag tttctgtaac cagctcatga tgtaagccct ttattatgcg
     6241 tcaatcgcac cacgcaactc agctgtaaga ctggccaagt tgccggcaac attttccgtg
     6301 gacgaattct cgatttcctg caccatgcgg ctgcccacca ccacggcatc gccaatcgcg
     6361 gcaatggcct tggctgtggc tgcatcgcgc acaccgaagc ccacgccgac cggcaattcg
     6421 gtatgctgac gaatggcggc aaccttgtgc gcgacatcgg ccacgttcag atggccggca
     6481 ccggtcacgc ccttgagcga gacgtaatag atgaaaccgc tggccaacct tacgatggca
     6541 tcaacgcgtg cagcctcggt ggtcggcgac aggagaaaaa tggggtcaat gccattttct
     6601 ttcagcttgg ccacgaacgc ttcgctctct tcgggcggat agtccactgt caacacacca
     6661 tcaacgccgc tctccttcgc cgaacttgca aatttctcat agcccatggc ctcgaccgga
     6721 ttggcatagc ccatcagcac caccggggtg gattgatcct gctggcgaaa ctcggccacc
     6781 atacccagca cgtcgcgtag cctgacatga tgcgccaatg cacgttcgct ggcgcgctga
     6841 atcaccgggc cgtccgccat ggggtcggaa aacggcacgc ccagttcgat aatgtcggcg
     6901 ccgctcgcta ccagccggtg catcagttca acggtaagac cggggtgtgg gtcgccggcg
     6961 gtgatgtagg gaatcagggc tttcctgccc tgttttttca agatttcaaa cgtagattga
     7021 atgcgcgaca ttagagcttg attccggaaa ggttggccac tgtgttgatg tccttgtcgc
     7081 cacggccgga gaggttgacc agcaggatct ggtcgcgtgc catggtcggt gccagcttgg
     7141 cggcgtaagc cagcgcatgg ctggactcca gtgccgggat gataccttcc atacggcaca
     7201 gggtatggaa cgcctgcaat gcctcgtcgt cggtcaccgc tacgtattcg gcacggtgca
     7261 agtccttcag ccaggcgtgt tccgggccga cgccgggata gtccaggccg gcggaaatgg
     7321 aatgggtttc gatgatctgg ccatcctcgt cctgcatcag ataggtacgg ttgccatgca
     7381 gcacgcctac cggactgttg gcggtgagcg gtgcggcatg cttgccggtc tccatgccta
     7441 ggcccgcggc ctcgacgccg atcagtcgta cgttttcctc ttcgatatac gggtaaaaaa
     7501 tgcccatggc gttggaaccg ccgccgacgc aggcgattac ggcatccggc tggcggccgg
     7561 tgagttccgg catctgctgt ttggcctcga tgccgataat ggactggaaa tcgcgcacca
     7621 tcatggggta aggatgcggt ccagccaccg tgccgatgat gtagaaggta ttagagatgt
     7681 tggtcaccca gtcgcgcatg gcttcgttga gcgcatcctt gagggtcttg gaaccggatt
     7741 cgaccggcac cacggtggcg cccagcagtt tcatgcggaa cacgttgggc gcctggcgct
     7801 tgacgtcttc cgaacccatg tagaccacgc attccagccc catgcgcgcc gccacggtgg
     7861 ccgtcgccac gccatgctgg ccggcgccgg tttcggcgat gatgcgcggc ttgcccatgc
     7921 gcttggccag cagcgcctgg ccgatacagt tgttgacctt gtgcgcgccg gtgtgattga
     7981 ggtcttcgcg cttgaggtag atctgcgcgc cgccgaagtg atcggacaga cgcttggcgt
     8041 ggtagatcgg gctgggacgg ccgacgaagt gcttcaggtc atgctcgaat tcggccagga
     8101 actccgggtc gtggcggtag cgctcgtaca tcgcgcgcaa ttcctcaagg gcctcgatca
     8161 gcgtctccgc cacaaagatg ccgccatagg ggccaaaatg gccgcgggca tcgggaaagt
     8221 catacacctg catcaagcac tcctcgcatg aacgcggcga ttttgtccgc gtccttgatt
     8281 cctttttctc gttccacccc gccgctcaca tccaccgcat agggacgcac gcgccgaatg
     8341 gctgcgccga cgttgttggc attcaagccg ccggccagaa taaccggctt gggcaggttt
     8401 gaaggaatca aatcccagtc gaacacctgc ccggtgcctc ccgccacacc ttcctgccag
     8461 gcatccagaa gcaatgcccc ggccgaggca aaatcggctg cgtattgtac caaatttagc
     8521 tctggcctga cgcgtaccgc cttgatatag gggagattga aggactggca gtattcggga
     8581 gattcatcgc catggaattg cagcaagtcc agtctcacct tggcaagcac agcgcgtact
     8641 ttggctggcc cggcatccac gaacagacct accgcggcta cgaacggcgg caagctcgcc
     8701 acgatctcag ccgcacggtc aacgccgaca tggcgcgggc tgttggcata gaacaccaag
     8761 ccgatggcat cggcgccggc ggcaacagcc gcatcagcgt cctcgacgcg ggtaatgccg
     8821 cagattttgg tacgagtgcg catattcgac aagcttagtc cttcagagca tagatgcccg
     8881 gcagattacg ccaccagccg tgcacatcca tgccacagcc aaatacgtag caattgggta
     8941 cggtcagacc gacgaaatcg gcctgcaccg gcttgggtcg gccggtttcc ttggttgtca
     9001 gcaccgcgat cgcaacgcgg gaagcacctg cttccaggca tttttgccgc accgcggcca
     9061 aggtatggcc ttcgtccagg atgtcgtcca gcagcagcac ggtacggcca cgcacatttt
     9121 cgcccggcgt gactttccag atcagatgct gcccgctggt accgttgtgg taacggctgg
     9181 cctgcacgta atcaaactcc agcgggaatt gcagccttgg cagcagccag ccagagaaca
     9241 ccaccgcccc gcccatcaca catagcacca gcggcgcact gtcgcctaac ttttctgtaa
     9301 tctcctgcgc cagtcggtgc actgccgcgt ccacttgcct ggattcatag atcatttccg
     9361 cgttctgcag aagttgctca ggagagatgg atgaggacat gctatctcgg cacggtacgc
     9421 aaataatcgg caaatttcct gccgatttcc ggatggcgca tggcgaactc gacattcgcc
     9481 tgcatgtagc caagcttgct gccacagtcg tagcgcctgc cttggaatgg gtaggccagc
     9541 acctgctctt gcgccatcag tgcagcgatg ccatccgtca gctgaatttc gccacccttg
     9601 cccggcgaca acgcctccag acagtcgaaa atgcgcggat tgaggatgta gcgacccacc
     9661 acggccaagg tggaaggcgc ttcctgcggc tgcggttttt ccacgatacc gcgcacttcc
     9721 agcaggctgg ccgcgatccc atgggcatcg acgataccgt agccggaggt ttcgctgcgc
     9781 gccacggttt ccactccgag cagggatttg ccgtattgcg catacaagtc ggtcatctgc
     9841 ttcagtaccg gcggattgcc gtcaatcaga tcgtcggcca gaatgaccgc aaacggctcg
     9901 tcacccacca ccggacgtgc gcacagcacg gcgtgcccca ggcccagcgc ctcggcctgg
     9961 cggatataga tgcaactgac atggctgggc aggatgccac gcagttgttc cagcattttc
    10021 tgccggccgc gcgactccag ttccgcttcc agttcgtagg ccttgtcaaa gtgatcctcg
    10081 atggcccgct tgttgcgtcc ggtgataaaa atcagctcgg tcacgcccgc cgcaatcgct
    10141 tcctctactg cgtactgaat cagcggcttg tcaacgacag gcagcatttc cttggggctg
    10201 gccttggtcg ccggcaggaa gcgcgtcccc ataccggcca ccgggaatac tgccttggtg
    10261 atcttgttca atgcggctcc ttcaatgcac cgttgtcgaa acgggcacca tgatcgagtc
    10321 gggcgaaaca ctgtggatat cgcccgtgta ttcctcgacc caggttttca gttcctgctt
    10381 gacgacgggc tcctccatgc gggcagcgct ctccacccat tgcagcaggg ccgccaccca
    10441 atcggaattg gcgccgcgcg cgcgcgcgat gcgcagcttg ggatgggggg ttggcagggt
    10501 atgctcgtcg tcggccagca attcctcgta caatttttcc cccggccgca gaccggtgta
    10561 gacgatcttg atgtcatcct cgccaaaacc ggacaggcgg atcatctcct gcgccagatg
    10621 ggcgatacgg atcggctcgc ccatgtccag cacgaaaatt tcgccgccat cgcccatcag
    10681 gccggcctgc agcaccagtt gcgccgcttc cgggatggac atgaaatagc gcgtgacctc
    10741 gggatgcgtc accgtcaccg gcccgcccct ggcgatctgc tcgcggaatt tcgggatcac
    10801 gctgccggag gaccccagca cgttgccgaa acgcaccatg acgaagcggg taccgccctc
    10861 ctcctgcagg ccctgacaag ccatctcggc cagccgtttg ctggcgccca tgacgttggt
    10921 cggattgacc gctttgtcgg tcgagaccag caccatcttg cccacgccct cctgcttgca
    10981 ggcggcggct acggcatggg tgccaagtac attgttggcc aacgcctccg acacgttctc
    11041 ccactccatc agcggtacat gcttgtaggc ggcggcgtgg aacaccacct gcggccggaa
    11101 gcggcgcaac actccggcca ctcgttcggc attcctgaca tcgccgacga cacaggcaat
    11161 gtgctggcgc ggaaaatttt cccgaaattc ctgctccagc tggtacaggg caaattcgtt
    11221 caattcaaac aacaccagtg tctcggggtt gaaacgcgaa atctgccggc acagttctga
    11281 gccgatcgat ccgcctgccc cggtcaccag caccacgcgg ttgctgatga ggctgtgcag
    11341 cccggcatca tccagttgca ccggatctcg ccccagcaaa tcctctactt ccaccctgcg
    11401 cacctgcgat acgctgacct tgccgctggc gagatcgtcg aacgagggca ccgtcagcac
    11461 cgccaatccc gcctgctcgg caatatcgat ggcgcgccgc cgcacagaat gggataccga
    11521 gggcatagcc aggatgacat gggatatccc cattctggca gcatatatcg gcgcttcact
    11581 caggctaccc agcactttta caccataaat gtggcgccca tgcatgccgc tgctatcgtc
    11641 aagcaacccg accacgcgcc agtcacggct gcgctccatt tccttgagca gggatactgc
    11701 ggtgttgccg gctcccatca ccagcacggg ctcaccttgc aactgtctgg ggccgtagag
    11761 gtgatgctcc ttccaggcgc gataaagaaa acggctgccg cccatcatca gaagcagcag
    11821 gatagggtcc agcagcagta ccgagcgcgg caccacgtag gactggcgca aaaacggcaa
    11881 cagcgccgcc actgccagcg cggctacgcc aactgccgtg agaatgcgct tgagatcagg
    11941 gatgctggcg aagcgccaga tgcctcgata caggccaaat ttaagaaaga tagccgattg
    12001 cagcaacagc acccagggca aggtatgcag aattgcactg tcagcatagt ccggcaggga
    12061 gaaattgaac cgcagcaggt aagccaatac ccaagccatg gccgcagcga aaacatcgtg
    12121 cacaaaaacc aggaaggcgc gcggcaggcg gtcaattttc attgatttgc aacatgtatc
    12181 ttcgggaggt gaagtttaaa ccacctgcgg tccaccgcga tagccaatcc gcagtatatc
    12241 gctccccata gcagcaaacc cgccagttgc agcggccagg cctggcttgc cagacataag
    12301 gccgaccctc cggccagagc catcaagcca tactcggcca gagcggtgct gcgatgtccc
    12361 cagcccattt gcaccagcct ttggtaatag tggctgcgat gtgcctgcca gactttttct
    12421 ccgcgcagca tgcgtttgac cagggtcaca gttgcatcgg cgataaatgg cgagaatacc
    12481 agcagcggga accacagcgg ccagtcgccc tgctgccatc ccgacagtcc caatgcagca
    12541 gccagaaaac ccaaggggac agaaccggca tcgcccatga aaattcttgc cggatggaaa
    12601 ttgaacagga ggaatgcggc agaagcggcg gcaacggcca gcgaagaaaa tgccaatcct
    12661 gcatctgcag cctgccacgc acccagcgcg taaaaggaga aaccgaacaa cgccatgccg
    12721 ccggccagtc catccgagcc atccatgaag ttgtagagat tggtcatcca gaccattgcc
    12781 accaacaaca ccggccacca ccacggatcc ggcgcggaca gcatggtcaa cccaagaaaa
    12841 accgccgctg cgagggaatg cgcagcaaac cgccatcctg ccgagaggct gtatatatca
    12901 tcgagaaagg acaacgccac cagaaacagc atgcagcagg tcaagattag caactctgga
    12961 tgccatgcca tcccccaggc agccacgatg ccagtcatga tcgccagccc gccggtacga
    13021 ggtacggcga ccgtatgcag cgaacgttcg ttaggctggt cgagcaccgt tctggcaatc
    13081 cttccattca ccagcagcaa aagaacagac aaagccacca cgaacgaccc tccggcagcg
    13141 agaatgaagt ttttggacat ggcgatgatg atggcggtaa atcagccgtt agtctgtcaa
    13201 tgcgggctct gccgctacat ccatgtacac ctcgacaccg gcagcgggcg cgacagccgc
    13261 taccggcagg tcggcgccac tacgccagag tttgacagca tcgcgtttgc cggcgccagt
    13321 cacgaagaaa atcacgcgat gcgcgcggga aagcctggac gcggacaacg acacgcgctc
    13381 cggtggaggc ttcggggcac catgtactgc gatggccggc gccatggctt gagattccca
    13441 agccttcccc ggaaacaggc tggctgtgtg cgcatcttct cc
//
LOCUS       DAOMFA010000027        13229 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_428_length_13229_cov_5.383862, whole genome shotgun sequence.
ACCESSION   DAOMFA010000027 DAOMFA010000000
VERSION     DAOMFA010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 13229)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13229)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13229
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_428_length_13229_cov_5.383862"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            166..3618
                     /gene="dnaE"
                     /locus_tag="PKW44_02545"
     CDS             166..3618
                     /gene="dnaE"
                     /locus_tag="PKW44_02545"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HQR50501.1"
                     /translation="MSQPVFVHLRCHSEYSVVDGMVRLADYVGRAVKDNMPALALTDL
                     SNLFGAVKFYSAARGKGVKPILGCDVWLENPANRDQPYRLLLLCQSQAGYLRLCELLT
                     RAYLTNQYRGRAELKRGWLVELGTEGLILLSAAEGGDVGQALLQDNTQQAQLLAAQWQ
                     ALFPGRYYLELQRTGQPQQEAVIARTLDLAVALGLPVVATHPIQFLDTDDHKAHEARV
                     CIAEGYMLADRRRPRHFTEQQYFKTQAEMAELFADIPEALQNSVEIAKRCNLTLTLGK
                     NYLPQFPTPNGESLDDYLAVQARAGLEQRLAVLYPDAAQRETRRVEYAARLEFEVGII
                     VQMGFPGYFLIVADFINWAKHNGVPVGPGRGSGAGSLVAYSLGITDLDPLRYALLFER
                     FLNPERVSMPDFDIDFCQEGRDRVIDYVKHKYGLEAVSQIATFGTMAAKAVIRDVGRV
                     LDLPFNFVDGIAKLIPNELGITLSDALEKEPQLKERREKEEEVNELLELALRLEGLTR
                     NVGMHAGGVLISPGRITDFTPMYCQQGSDSVVSQYDKDDVEAVGLVKFDFLGLRTLTI
                     LAMALDNTNAQRAAKGEPALSFETLPLDDAPTFDLLKAANTTAVFQLESRGMKDMLKQ
                     AKPDCFEDIIALVALYRPGPMDLIPDFCRRKHGLQRVEYPHPATAPILKETYGIMVYQ
                     EQVMQIAQVVAGYSLGGADMLRRAMGKKKKEEMDAQRAVFVEGAQKNGTAAHDAEFLF
                     DLMEKFASYGFNKSHAAAYALVAYHTAYLKKHYPAAFLAATMSADMNNTDSVHIFHDD
                     CLANGVEVLPPDINRSEFRFKPLSDKQVLYGLGAIKGTGWSAIEVILASREQDGPFKD
                     LFDFCLRLDLRKVNRRVIESLIRAGAFDQLEPNRAALLAGVGMAIEAAEQSGAAAGQN
                     SLFSDQPDISNHKLPQLQPWTEQEKLQQEKAALGFYFSGHPFLAYRNELTAFVRGGLA
                     TLTPQEQPQLLAGVVVAIRTRMTSRGKMAFVTLDDAGAQIEVAIGNELFMANTALLKE
                     DQLLFVEGRVSNDDFSGGLRVNARRLLDLATARGAYASRLRLSCNGQADAIRLRELLA
                     PYCKTARQEADGRGCPIQIDYRNQNARVELSLGEGWRVELRDELLHGLRTWLSEENVK
                     ILYH"
     gene            3656..4624
                     /locus_tag="PKW44_02550"
     CDS             3656..4624
                     /locus_tag="PKW44_02550"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HQR50502.1"
                     /translation="MKTSFLDFEQPIAELEAKIEELRYVQDDSALDISEEIDRLQQKS
                     QTLTKDIYAKLTAWQVSQVSRHPQRPYTLDYIQGLFTEFEELHGDRAYSDDPAIVGGL
                     ARFEGQSVMVIGHQKGRDTKEKIYRNFGMPRPEGYRKALRLYRLAEKFNLPIITLIDT
                     PGAYPGIGAEERGQSEAIARNLYVMAELKVPIIGVIIGEGGSGGALALGVVDHLMMLQ
                     YGTYSVISPEGCASILWKSADKAPDAAETLAITANRLKTMGLVDRIVAEPLGGAHREP
                     ELMMQSLRRALKDELQILQAKSTSNLLEARLDRLMSYGKFKEAAVR"
     gene            4593..5930
                     /gene="tilS"
                     /locus_tag="PKW44_02555"
     CDS             4593..5930
                     /gene="tilS"
                     /locus_tag="PKW44_02555"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HQR50503.1"
                     /translation="MASSRKLRSAELPARLESFLKQAVRPGQTLLLGLSGGLDSRVLL
                     DLLQRLRATAGFHLQALHVNHGISPKAAEWEGFCADLCASLAIPFQTVCVDIPRDSGL
                     GLEATAREARYAVLLAQPVDAVVLAHQRDDQAETLLLQLLRGAGLKGLAAMPVIRASA
                     NGQPVLLRPLLDVPRDVLEDYARGHQLRWIEDESNLDLTFDRNFLRHHIFPELERRFP
                     ASRTTLARSASHLAEADALLREVAEADAGRLVRDGRLEVAGLAAMSEARAKNLLRYWL
                     DSHGAATSSRRLQEIYRQLLHARAEAQISIHVDGGTLHRYRGQACYEPMDGAVTLPEL
                     CWQGEPELVLPGGRLAFTRATGQGLSLARSGAGELRVRTRTGGERFRPDCRRPTRDLK
                     ALFQQAGLPPWQRQHIPLLYLDDTLVAIPGIGVACGWQTAVAEEGVVINWLAS"
     gene            6178..8520
                     /locus_tag="PKW44_02560"
     CDS             6178..8520
                     /locus_tag="PKW44_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQR50504.1"
                     /translation="MHNNHIFRMRTVAMMVAAICAAPQAVLADDDKQVVETGAVEVVG
                     TTPLAGIGVSIDQVPSNVQVVKAKALEEQQSLGVADYMLENLLGVSVNDTQNNPYQPD
                     VNYHGFTASPLLGTPQGLSVYQDGVRINEPFGDSVNWDLIPSNAISGITLMPGSNPLF
                     GLNTLGGALSIQTKSGITNPGGALQAYGGSWERRAVEAEYGGTVENGANYFFSGNVFS
                     EDGWRDYSHSNVRQYFGKLGWAGDKTDFNVSLTGANNELKGNGYTPHSFLRQNYDSVY
                     TQPDVTSPELLFFNAQFNHWISDNLVFNSVAYYRHNQIKTYNADLNDGYDDTDPGFGT
                     DPDVTDQGVINRTKSTENGYGLAGQLNWNTAANLLIGGASYDRGNIDFKQTSQLFSSW
                     DASRGVGGVVDDDVNEEVDMNGRTYTSSLFATDTYKLTSQWAVTASARYNYTNVSNHD
                     NLIPQAGTGSLTGQQAFHRVNPALGATFTPNASLNFFGGYNEGSRAPSSIEIACSDPA
                     SPCLLPNSMAGDPPTLDQVVAKTWEGGVRGRLGSGVQWSLGAYRTNSYDDIQFIASNT
                     TGAGYFDNVGKTRRVGLDMGLNGVLGKFRWNAGYSYVKATYESQFTMLAEANSQSGTD
                     CQGAAVAGAICVNKGDKMAGIPEHQFKLRGEYSLLPNWVAGGNLVAFSDQYARGNENN
                     ADPNGKISGYAFLNLDTRYNFGSSGWQLFAKVNNVFDREYYTGGILGENAFTGAGNSF
                     SANSGNWEDERFLAPGAPRAGWVGLRYEFGGAKGSGASLD"
     gene            8582..9958
                     /locus_tag="PKW44_02565"
     CDS             8582..9958
                     /locus_tag="PKW44_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HQR50505.1"
                     /translation="MDQKLRVLLIEDETLFARAVAKRLQQAGYDCERAETLADGRAVA
                     RQFQPELVLLDMRLPDGNGLDLLSEFAAKGVTVIVMTAFGEINDAVGAMKQGATDYLK
                     KPVDLEELLLAVEKAQDSTRLKHQLDYSRQRDSHAVEGVELLGESAVMQAVHAQVERI
                     ARLSSLADTIPPTVLISGETGTGKDVAARLLHQSCANHERPFVHVDCASLPAELIESE
                     LFGHEKGAYTSAQGARPGLIEAAEDGTLFLDEIGELPLTLQAKLLNVLERRVVRRLGS
                     TRERQVPARFVAATNRDLQQMVLDGRFRADLYYRLNVLGVTMPPLRQRGDDIVSLARH
                     FAAQTERRYGLPQREFSVEAIQAISNYSWPGNVRELKHQVSRAVLLSKGATVSEDDLL
                     LPFHVHASAPAGEAASAVTLDEAEKVLIENALKQAGGNVSEAARLLGITRMTIRYRMD
                     KHGIKSGD"
     gene            10004..11203
                     /gene="purT"
                     /locus_tag="PKW44_02570"
     CDS             10004..11203
                     /gene="purT"
                     /locus_tag="PKW44_02570"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HQR50506.1"
                     /translation="MNIGTPLSPSATRVMLLGSGELGKEVIIALQRLGVEVIAVDRYP
                     NAPGHQVAHRSHTISMTDGAALRALVEQEKPHLIVPEIEAIAIDALAEIEASGLARVI
                     PTARAVQLTMNREGIRRLAAEQLKLLTSPYAFASSLQDMQAAIDGGIGYPCFIKPVMS
                     SSGKGQSRVTAPDQVSAAWDYAASGGRVDQGRVIVEGQIDFDYEITQLTVRAVSATGA
                     VETYFCDPIGHVQIKGDYVESWQPQPMSQKALQRSREVAKAVTDDLGGLGLFGVELFV
                     KGDEVWFSEVSPRPHDTGMVTMASQRFSEFELHARAILGLPVDVAMRAPGASAVIYGG
                     VDSDNLVYEGVDEALAVPESDLRLFGKPQAFQRRRMGVALATGKDVEEARVRAKLAAS
                     KVRPLTA"
     gene            11200..11604
                     /locus_tag="PKW44_02575"
     CDS             11200..11604
                     /locus_tag="PKW44_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II truncated hemoglobin"
                     /protein_id="HQR50507.1"
                     /translation="MSEQPATFYEVVGGEEGLRRLVKRFYEIMDSEPRAAGIRAMHKP
                     DLSEANEKLFLFLSGWMGGPPLYVEKYGHPMLRARHLPFPIGESERDQWVYCIVRAMH
                     DVGLEEKAMLQLGNALWGVADFMRNQPETEKA"
     gene            complement(11633..12994)
                     /locus_tag="PKW44_02580"
     CDS             complement(11633..12994)
                     /locus_tag="PKW44_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HQR50508.1"
                     /translation="MSTDITTFASLNLAPEILRAVEEEGYTKPTPIQAQAIPLVLDGR
                     DLMAGAQTGTGKTAAFTLPLLQKLLPHASTSASPARHPVRALILTPTRELAIQVEESV
                     QAYSKHVPLRSLVVFGGVDIKTQTPALRNGVEILVATPGRLLDHVEQRTVQLNQVQML
                     ILDEADRMLDMGFLPDLKRILALLPKQRQNLMFSATFSNDIKKLADDFLTNPTLVEVA
                     RSNAASENVEQKIYRVAQADKHALLAEILRRDNVQALVFTKTKLTTGRLARMLQRDGI
                     AADAIHGDKSQQERLQALEDFKQGKITTLVATDVAARGLDIDQLPLVINYEIPHAPED
                     YVHRIGRTGRAGASGVAISLVAPEEEKYLKEIEKLIKREFHEEKAPQPQRSTPPQRRT
                     EREPGRSSATAPVRKSSSDPWFDRPYEPDSSNAPVRHTPRRTTKQKREVAALLGGLLQ
                     KTE"
     gene            complement(13004..>13229)
                     /locus_tag="PKW44_02585"
     CDS             complement(13004..>13229)
                     /locus_tag="PKW44_02585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR50509.1"
                     /translation="LEVAVIAGLEPKRIQQPNPHKFGDSGKPRNSARPANKAGGYKGA
                     GSRDAGQGFGGQKKRDGDKSFSKRGGRNFV"
BASE COUNT         2596 a         3895 c         4099 g         2639 t
ORIGIN      
        1 ggtaggcttg cagacgccag ggatttttac cagcgcagca aggccttggc cgacaggatc
       61 ggcgcgcagg atttgatcga tgagatcggc gatacgcttg cagcaggcgg gagtaaatag
      121 cccctgcgat gaatttttag aagcgccctg aggtaaaatt caggcatgag ccagcccgtt
      181 tttgtccacc tgcgttgcca cagcgagtat tccgtggtgg acggcatggt gcgcctggcc
      241 gactacgtcg ggcgtgcggt gaaagacaac atgccggcac tggcgctcac cgatctttcc
      301 aacctgttcg gcgcggtgaa gttctattcg gcggcgcgcg gcaaaggcgt taaacccatc
      361 ctgggctgcg atgtctggct ggaaaacccg gccaaccgcg accagccgta tcgcttgctg
      421 ctgctgtgcc agtcgcaggc gggctatctg cgcctgtgcg agctgctgac ccgcgcttac
      481 ctgaccaacc agtatcgtgg ccgcgccgag ctcaaacgcg ggtggttggt agagctcggc
      541 acggaggggc tgatcctgct ttccgcggcg gaaggcggcg atgtcgggca ggccttgctg
      601 caggacaata cccagcaggc gcagttgctg gcggcgcaat ggcaggcgct gttccccggc
      661 cgctattacc tcgaactgca gcgtaccggc cagccgcagc aggaggctgt catcgcccgt
      721 acgctggacc tggccgttgc gctgggttta ccggtagtcg cgactcatcc tatccagttt
      781 ctcgataccg acgaccacaa ggcgcacgag gcgcgggtgt gcattgccga aggctatatg
      841 ctggccgatc gccgtcgccc gcgccatttt accgagcagc agtatttcaa gacccaggcc
      901 gagatggcgg aactgttcgc cgatattccc gaggcattgc agaactcggt ggagatcgca
      961 aagcgctgca acctgacgct gaccctgggc aagaactacc tgccgcaatt ccccacaccc
     1021 aacggagaaa gtctggacga ttacctggcg gtgcaggcgc gtgccggcct ggagcagcgg
     1081 ctggcagtgc tttatcccga cgcggcgcag cgcgagacca ggcgtgtcga atacgcagcg
     1141 cgcctggaat tcgaggtcgg catcatcgtg cagatgggtt ttcctggcta tttcctgatc
     1201 gtggctgact tcatcaactg ggccaagcac aatggggttc ccgtaggccc tggtcgtggc
     1261 tccggcgctg gttcgctggt ggcttacagc cttggcatca ccgatctcga cccgctgcga
     1321 tacgccctgc tgttcgagcg cttcctcaac ccggagcggg tgtcgatgcc cgacttcgat
     1381 atcgacttct gccaggaagg gcgcgaccgc gtcatcgact acgtcaagca caaatacgga
     1441 ttggaggcgg tctcgcagat cgccaccttc ggcaccatgg cggccaaggc ggtgatccgc
     1501 gacgtcggcc gcgtgctgga tttgccgttc aatttcgtcg acggcatcgc caagctgatc
     1561 cccaacgagc ttggcatcac gctttccgac gcgctggaga aggagccgca actcaaggag
     1621 cggcgcgaga aagaagaaga agtcaacgaa ctgctggagc tggcactacg actggaaggg
     1681 ctgacacgca acgtcgggat gcacgccggg ggcgtgctga tctcgccggg acgcattacc
     1741 gacttcactc ccatgtactg ccagcagggc agcgacagcg tggtgagcca gtacgacaag
     1801 gacgacgtgg aggcggtggg cttggtcaag ttcgacttcc tcggcctgcg cacgctgacc
     1861 atactggcca tggcgctgga caataccaac gcccagcgtg ctgccaaagg cgagccggcg
     1921 ctgtcgttcg agactttgcc gctggacgac gcgccgactt tcgacctgct caaggccgcc
     1981 aacaccaccg ccgtgttcca gctggaatcg cgcggcatga aggacatgct gaagcaggcc
     2041 aagccggact gcttcgagga catcattgcc ctggtggcgc tgtaccgccc cggcccgatg
     2101 gacctgatcc ctgacttctg ccgccgcaag cacggcctgc aacgggtgga gtatccgcac
     2161 ccggcgactg cgccaatcct gaaagaaacc tacggcatca tggtgtacca ggaacaggtg
     2221 atgcagatcg cgcaggtggt ggcgggttac agcctgggcg gcgccgacat gctgcgccgt
     2281 gccatgggca agaagaaaaa agaagaaatg gatgcgcagc gtgccgtgtt tgtcgagggc
     2341 gcgcagaaga atggcactgc ggcgcacgac gccgagttcc tgttcgatct gatggaaaag
     2401 ttcgccagct acggcttcaa caagtcgcac gcggcggcct atgcgctggt ggcctaccac
     2461 acggcctacc tgaaaaagca ttacccggcg gccttccttg ccgccaccat gtcggccgac
     2521 atgaacaaca ccgacagcgt gcatatcttc catgacgact gtctggccaa tggcgttgaa
     2581 gtactgccgc ccgatatcaa ccgcagcgaa ttccgcttca agccgctcag cgacaagcag
     2641 gtgctgtatg gcttgggcgc catcaagggc accggttggt cggccatcga agtaattctg
     2701 gcctcgcgcg agcaggatgg cccgttcaag gatctgttcg atttctgttt gaggctggac
     2761 ctgcgcaagg tcaaccgccg cgtgatcgaa tcactgatcc gcgctggtgc tttcgatcag
     2821 ctggaaccta accgtgccgc cctgctggca ggcgttggta tggctatcga agcggcggaa
     2881 cagagtggtg cggcagcggg gcagaacagc ctgttcagcg atcagcctga catctccaac
     2941 cacaagttgc cacagctgca gccgtggacg gagcaggaaa aattgcagca ggaaaaagcc
     3001 gcgctgggtt tttatttcag cggccatccc tttctcgctt accgcaatga actgaccgcc
     3061 tttgtgcgtg ggggactggc gacactgaca ccgcaggagc agccgcaatt gctggctggt
     3121 gtggtggtgg cgatccgtac tcgcatgacc agccgcggca agatggcctt cgtcacgctg
     3181 gacgatgccg gcgcacagat cgaggtggcc attggtaacg agctgttcat ggccaatacc
     3241 gcactgctta aggaagacca gctgcttttc gtcgaaggcc gggtcagtaa tgacgacttc
     3301 agcggcggcc tgcgcgtcaa tgcgcgccga ttgctcgatc ttgccactgc gcgcggcgcc
     3361 tacgccagcc ggctcaggct gtcctgcaac ggacaggcag atgctatccg gctacgtgaa
     3421 ctgctggcgc catattgcaa gacggcgcgg caagaagcag acggccgcgg ctgtccgata
     3481 cagatcgact accgcaatca gaatgcgcgc gtggaactat cgctcggcga ggggtggcgg
     3541 gtagaactgc gcgacgagtt gctccatggc ctgcgcacct ggctttccga agagaatgtg
     3601 aaaatcctgt atcattgagc ctgattggcg ataagcgccg ctgaagagat tttttatgaa
     3661 aaccagtttc ctagacttcg aacaaccgat cgccgaactc gaggctaaaa tcgaagaact
     3721 tcgctatgtg caggatgatt ccgcgctgga catttccgag gaaatagacc gtctacagca
     3781 gaagagccag accctgacca aggacatcta cgccaagctg actgcctggc aagtgtcgca
     3841 ggtgtcgcgc cacccgcagc gcccttatac actggactac attcaggggc tatttaccga
     3901 gtttgaagaa ttgcacggcg accgcgccta ttcggatgac cctgctatcg ttggcggcct
     3961 cgcgcgcttc gaaggccagt cggtgatggt gatcggccac cagaaaggcc gggacaccaa
     4021 ggaaaagatc taccgcaact tcggcatgcc acgtccggaa ggctatcgca aggcgctgcg
     4081 cctgtatcgc ctggcggaaa aattcaacct gccgattatt accttgattg acacccctgg
     4141 cgcctatccc ggcatcggtg ccgaggagcg cggtcagtcc gaggccattg cacgcaacct
     4201 gtacgtgatg gccgaactca aggtgcccat tattggcgtc attatcggcg aaggtggctc
     4261 ggggggggcc ttagcgctgg gtgtggtcga ccacctgatg atgctgcagt atggcaccta
     4321 ttcggtgatc tcgccggagg gctgcgcctc gattttgtgg aaaagtgccg acaaggcgcc
     4381 ggatgccgcg gagacccttg ccattaccgc caaccgtctg aagaccatgg gactggttga
     4441 tcgcatcgtt gccgaaccct tgggcggcgc gcatcgcgag ccggaactga tgatgcaaag
     4501 cctacgccgc gcgttaaaag acgagctgca aatcctgcag gcaaaatcca cctccaacct
     4561 gctggaagca cgtcttgacc ggctcatgag ttatggcaag ttcaaggaag ctgcggtccg
     4621 ctgaactgcc agcccgacta gaaagcttcc tcaaacaggc cgtccgcccc ggccagaccc
     4681 tgttgctggg gctatcgggc ggccttgatt cccgtgtcct gctcgacctg ctgcaacgct
     4741 tgcgggccac ggcgggattc cacctgcaag ccctgcatgt caaccacggc atcagtccga
     4801 aagccgccga gtgggaaggc ttctgtgctg atctgtgtgc ttcgttggcc attccattcc
     4861 agacggtgtg cgtcgacatc ccgcgtgaca gcggcctggg gctggaggcg acggcgcgcg
     4921 aggcgcgcta tgcagtgctt ctggcgcagc cggtagatgc tgtggtgctg gcccatcagc
     4981 gtgacgacca ggcagagact ctcctgctgc aactattacg cggcgccggc ctcaagggac
     5041 tggctgccat gccggtcata cgcgcgagcg caaacgggca gccggtgttg cttcgcccat
     5101 tgctggatgt gccgcgtgat gttctggagg actatgctcg cggtcatcaa ctgcgctgga
     5161 tcgaggacga aagcaatctc gatctgactt tcgaccgcaa tttcctgcgc catcacattt
     5221 tccctgaact ggaacggcgt tttcccgcca gccgcactac actggcacgt tctgccagtc
     5281 atctggccga ggctgatgct ttgttgcggg aggttgccga ggctgacgca ggtcgcctgg
     5341 tacgggatgg ccggttggag gtggcgggtt tggcagccat gagcgaagcg cgggcgaaga
     5401 acttgctgcg ctactggctg gacagtcatg gcgctgccac ttccagccga cgcctgcagg
     5461 aaatctaccg ccagttgttg catgcgcgag ccgaggcgca gatcagcatt catgtcgacg
     5521 gtggcacgct gcatcgctat cgtgggcaag cctgctacga gccgatggac ggcgcagtta
     5581 ccttaccgga actgtgctgg cagggcgagc cggaacttgt tctgccgggg gggcgcctcg
     5641 cttttaccag ggcgaccggg caagggctgt cgctggccag gtcgggagcg ggcgaattaa
     5701 gggtgcgcac acgtacgggc ggtgagcgct tccgccccga ttgccggcgt cccacacgcg
     5761 atctcaaggc gctttttcag caggcggggc tgccgccgtg gcagcgacaa cacatcccct
     5821 tgctttattt ggacgatact ctggtcgcta tccccggcat cggcgtggcg tgcggttggc
     5881 agacggcagt ggctgaagaa ggggtcgtca tcaactggct ggcatcgtag caggcacgcc
     5941 ccttgtttct ctagccatcc catgagagac gggcaccgcc catccggagt ggttgtatac
     6001 agccgttata tggctgtata caaccaccag cttccggtaa actgcaacca tggttgccgg
     6061 tcattcaatt aagcgcatga aaacaaaaag atgattctgc tcgtccctgg tatggcatgg
     6121 aatttgatag ctctactacg tctgactttg acgcattcat cgatttggag agaattcatg
     6181 cacaacaatc acatattccg catgcgtacg gtagccatga tggtggcagc catctgtgcc
     6241 gcaccacagg cggttttggc cgacgacgac aagcaagtgg tggaaaccgg cgcggtcgag
     6301 gtggttggca ccacgccatt ggccgggatc ggggtcagca tcgaccaggt gccttccaat
     6361 gtgcaggtgg tcaaggccaa agcgctggag gagcaacaaa gcctgggcgt tgccgattac
     6421 atgctggaaa atctgctggg ggtcagcgtc aacgacaccc aaaacaatcc ctatcagcct
     6481 gacgtcaact accacggttt taccgcttct ccgttgctgg gaacaccgca aggcctgtcg
     6541 gtctaccagg atggcgtgcg catcaacgaa ccattcggcg attcggtaaa ctgggacctg
     6601 attcccagca atgcgatttc cggcattacc ctgatgccgg gctccaatcc tctgttcggc
     6661 ctgaataccc ttggtggtgc gctgtccatc cagaccaaga gtggtattac caatcccggc
     6721 ggggcactcc aggcgtatgg cggctcctgg gagcgccgtg cggtggaggc ggaatatggc
     6781 ggcacggttg aaaacggcgc caactatttc ttttccggca acgtgttcag cgaggatggc
     6841 tggcgtgact actcgcatag caacgtgcgc cagtatttcg gcaagctggg ctgggcaggc
     6901 gacaagactg atttcaacgt cagcctgacc ggcgccaaca atgagctgaa aggaaatggc
     6961 tatacaccgc attccttcct gcggcagaac tacgattctg tgtataccca gccggatgtc
     7021 accagtcccg aactgctgtt cttcaatgct cagttcaacc actggatttc tgacaatctt
     7081 gtgttcaaca gcgttgccta ttaccggcat aatcaaatca agacctataa cgccgacttg
     7141 aacgatggtt acgatgatac cgacccgggg ttcgggacag acccggacgt gaccgaccaa
     7201 ggcgtcatca accgtaccaa aagcaccgag aatggttatg gcctcgctgg ccagttgaac
     7261 tggaataccg ctgccaatct gttgatcggc ggtgccagct atgatcgagg caacatcgat
     7321 ttcaagcaga ctagtcaact gttcagcagc tgggatgcat ctcgtggcgt aggcggagtc
     7381 gtggatgacg atgtcaacga agaagtcgac atgaacggca gaacttatac gagcagcctg
     7441 tttgccacgg acacttacaa gctgacctcg cagtgggcgg tcaccgcctc tgcgcgctac
     7501 aactatacca atgtgagcaa ccacgacaac ctgatcccgc aggccggcac cggttcactg
     7561 acaggccagc aggctttcca ccgcgtcaat ccagcattgg gtgcaacctt tactcccaat
     7621 gccagcctca atttcttcgg cggctacaac gagggcagtc gcgcgccatc ttccatcgaa
     7681 attgcctgtt ccgatccggc atccccgtgt ctgctgccaa actccatggc cggcgacccg
     7741 ccaacgctcg accaggtggt ggccaagacc tgggaaggcg gggtgcgtgg ccggttaggt
     7801 agcggcgtgc agtggagcct cggcgcctat cgcaccaaca gctacgacga tatccagttc
     7861 atcgccagca ataccaccgg tgcaggttat ttcgacaacg tcggcaagac ccgtcgcgtc
     7921 ggtctggaca tgggtttgaa tggggtcctg ggtaaattcc gctggaatgc cggatacagc
     7981 tatgtcaagg ccacctacga gtcccagttc accatgctgg ccgaagcaaa ttcgcaatct
     8041 ggcaccgact gtcagggtgc cgccgttgct ggcgccatct gcgtcaacaa gggtgacaag
     8101 atggccggca tccccgagca ccagttcaag ctgcggggcg aatacagcct gcttcccaac
     8161 tgggtggcag ggggcaacct ggtcgctttc tccgaccagt acgcgcgcgg caacgaaaac
     8221 aatgccgatc ctaacggcaa gatctcgggc tatgccttcc tcaatctgga cacccgctac
     8281 aacttcggca gcagcggctg gcagttgttc gccaaggtca acaatgtctt cgaccgtgag
     8341 tactacaccg gcggtatcct gggcgagaat gcgtttactg gcgcaggaaa ttccttttcc
     8401 gccaatagcg gcaattggga agacgaacgc ttcctcgcgc ctggcgcccc gcgtgccggc
     8461 tgggttgggc tgcgttacga gttcggtggc gccaagggct ccggcgcaag tctggactga
     8521 agagggttag cattggcaca tgcaggcacg cgaaattttt gagggcagat tgccattggc
     8581 aatggatcag aagttgcgtg tactgctgat cgaggatgag acattgttcg cccgcgcggt
     8641 tgccaagcgc ttgcagcagg cgggctacga ttgcgaacgc gccgaaacac tggcggatgg
     8701 ccgtgcggta gcccggcagt tccagcccga actggtatta ctggatatgc ggttgccgga
     8761 tggaaacggt ctggatctgc tctccgaatt tgctgccaag ggcgtcactg ttatcgtcat
     8821 gactgctttc ggcgaaatca acgatgccgt tggcgccatg aagcagggtg cgacggatta
     8881 cctgaagaag ccggtcgatc tggaagagtt gttgctggct gtcgaaaaag cgcaggattc
     8941 aaccaggctc aaacatcagc ttgactattc ccgccagcgt gacagccacg ctgtcgaagg
     9001 ggtagagttg ttgggtgaaa gtgccgtgat gcaggcagta cacgctcagg ttgagcgcat
     9061 tgccaggctg agttcgctgg cagacaccat tccgccgacc gtgcttatca gcggcgaaac
     9121 tggcaccggc aaggatgtgg cggcgcgcct gttgcaccag tcctgcgcca accacgagcg
     9181 gccgttcgtg cacgtggact gcgcctcgct gccggcggaa ttgatcgaaa gtgaattgtt
     9241 cggccacgag aagggtgcct acaccagtgc gcagggcgca cgccccggac tgatcgaggc
     9301 ggccgaagac ggtacgctgt tcctggatga aataggcgag ttgccgctga ccttgcaggc
     9361 caagctgctc aatgtgctcg agcggcgcgt ggtgcgccgc cttggctcca ccagagagcg
     9421 acaggtgccg gcacgcttcg ttgccgccac caaccgcgat ttgcaacaaa tggtgctgga
     9481 tggccgcttc cgcgctgatc tttactaccg cctcaacgtg cttggcgtca ccatgccgcc
     9541 gctcagacag cgtggtgacg acattgtgtc gctggcacgt cacttcgctg cccagaccga
     9601 gcgccgctat ggcttgcctc aacgcgaatt ctccgtcgag gcgatacagg cgataagcaa
     9661 ctattcctgg ccgggcaacg tgcgcgaact caagcatcag gtgagccgcg ccgtgctgct
     9721 gagcaagggc gccacagtct ccgaggacga tcttttattg cccttccacg tgcatgcatc
     9781 tgcgccagcg ggcgaagccg cttccgcggt cactctggac gaggcggaga aagtgctgat
     9841 tgaaaacgcg ctgaaacagg ctggcggcaa tgtctcggaa gccgcacgcc tgcttggtat
     9901 cacccgcatg accatccgtt accggatgga caagcatggc atcaagagcg gcgactagcc
     9961 ccgcgtggta aaatctccgc aaccaccctg tagcggagcc ctcatgaaca tcggtacccc
    10021 cctcagcccc tcggctacgc gcgtcatgct gctcggcagc ggcgaactgg gcaaggaggt
    10081 catcatcgcc ctgcaacgcc tgggcgtcga agtcatcgcc gttgatcgct accccaacgc
    10141 ccccggccac caggtcgcac accggtcgca taccatcagc atgaccgatg gcgccgccct
    10201 gcgtgcgctg gtagagcagg aaaagccgca tctgatcgtg ccggagatcg aagccattgc
    10261 cattgacgct ctggccgaga tcgaggcttc tggtctggca cgtgtcattc ctaccgcacg
    10321 tgcggtgcaa ctcaccatga accgcgaggg tatccgccgc ttggctgctg agcagctgaa
    10381 actgctcact tctccctatg ccttcgcttc cagcctgcag gacatgcagg ctgccattga
    10441 cggcggtatc ggctatccat gcttcatcaa gccggtgatg tcgtcttccg gcaagggcca
    10501 gtcgcgcgtg accgcacccg accaagtgtc cgccgcctgg gattacgccg ccagcggtgg
    10561 acgcgtggac cagggcaggg tgatcgtgga agggcagatt gatttcgact atgagatcac
    10621 ccagttgacc gtgcgcgctg tcagcgcgac cggtgcggtg gaaacctatt tctgcgatcc
    10681 catcggccac gtacagatca agggcgatta tgtggaaagc tggcagcccc agcccatgag
    10741 tcagaaggcg ctgcagcgct cgcgcgaggt cgccaaggca gtcaccgacg acctgggtgg
    10801 ccttggcctg ttcggcgtgg agttgttcgt caagggtgat gaggtgtggt tttccgaagt
    10861 cagcccgcgc ccgcacgata ccggcatggt gaccatggcc agccagcgtt tttccgaatt
    10921 tgaattgcat gcccgcgcta ttctcggcct gccggtggat gtcgctatgc gcgcccccgg
    10981 cgccagcgct gtaatctacg ggggggtgga cagcgacaat ctggtgtacg agggcgtgga
    11041 cgaggcgctg gcggtgccgg aatccgacct gcgcctgttc ggcaagccgc aagcctttca
    11101 gcgccgtcgc atgggtgtgg cgctggccac cggcaaggat gtcgaggaag cgcgtgtgcg
    11161 tgccaagctg gcggcaagca aggtgcgccc gttaacggca tgagtgagca gccggcaaca
    11221 ttctacgagg tagtcggcgg tgaagaaggt ctacgtcgct tggtgaaacg cttctatgaa
    11281 atcatggaca gcgaacccag ggcagccggc atccgtgcca tgcacaagcc ggacttaagt
    11341 gaggcgaatg aaaagctgtt cctgtttttg tccggatgga tgggcgggcc gccgctttat
    11401 gtcgagaagt atggtcaccc catgctgcgg gcacgtcacc tgccattccc gatcggggaa
    11461 agcgaacgcg accagtgggt gtattgcata gtgcgtgcca tgcacgatgt cggcctggag
    11521 gaaaaagcga tgctgcagct gggaaacgcg ctgtggggag tggctgattt catgcgtaac
    11581 cagccggaaa cggaaaaagc gtagcgcagg cggaaccgcc cgcgctactg aatcactcgg
    11641 ttttttgtag caggcctcca aggagggcgg caacttcccg tttctgcttg gtcgttcggc
    11701 gcggggtgtg ccgaacggga gcgttgctac tatccggttc gtaagggcgg tcgaaccagg
    11761 ggtcgctgct tgatttcctg acaggagccg tagctgaact tctgcctggt tcgcgctccg
    11821 tgcggcgttg tggaggagtg ctgcgttgtg gctgtggtgc cttttcctcg tgaaattcgc
    11881 gcttgatcag cttttcgatt tccttcaggt atttctcttc ttccggtgcc accagcgaga
    11941 tcgctacgcc cgatgcccca gcgcggccgg tacggccgat gcggtggaca taatcttccg
    12001 gtgcgtgcgg gatttcgtag ttgatgacca gcggcaactg atcgatatcc agcccgcgcg
    12061 cggcgacgtc ggtggccacc agcgtcgtga tcttgccttg cttgaagtct tccagcgcct
    12121 gcaggcgctc ctgctggctc ttgtcgccat ggatagcgtc ggcagcgatg ccgtcgcgct
    12181 gcagcatgcg cgccaggcgg ccggtggtga gcttggtttt ggtaaacacc agtgcctgca
    12241 cgttatcgcg gcgcaggatt tcagccaata gcgcgtgctt gtcggcctgt gccacgcgat
    12301 atattttttg ctcgacgttc tctgacgcgg cgttgctacg cgcaacttct accaaggttg
    12361 ggttggtcag gaaatcgtcg gccagcttct tgatgtcatt ggagaaggtg gcagagaaca
    12421 tcaggttctg gcgttgcttc ggcagcaagg ccaggatgcg cttgaggtcg ggcagaaagc
    12481 ccatgtccag catgcggtcc gcttcgtcca gaatcagcat ttgcacctgg ttgagttgca
    12541 ccgtgcgctg ctcgacgtgg tcgagcaatc gtcctggtgt cgccaccagg atttccacgc
    12601 cgttcctgag tgctggcgtc tgtgtcttga tgtcgacccc gccgaacacc accagcgagc
    12661 gcagcggcac atgcttgcta taggcctgca cgctttcttc cacctggatc gccagctcgc
    12721 gcgtcggtgt caggatgagc gcgcgcaccg gatgccgtgc aggcgaagca ctggtgctgg
    12781 catgcggcag cagtttctgc agtagcggca gggtgaaggc tgcggttttt ccggtgccgg
    12841 tctgcgcccc agccatcagg tcgcgcccat ccaacaccag cggaatcgcc tgtgcctgga
    12901 tgggcgtcgg tttggtatag ccttcttctt cgactgcgcg caggatctcg ggcgcgaggt
    12961 tgagggaggc aaaggtcgta atgtcggtac tcacggcggg caattatacg aaattccggc
    13021 cgccgcgctt actgaacgat ttatcgccgt cgcgtttctt ttggccacca aagccctggc
    13081 cagcatcacg actgcctgca cctttgtaac cgcctgcctt gttggccgga cgggcgctat
    13141 tgcgcggttt gccgctatca ccgaacttgt gtgggttggg ctgctggatg cgcttgggtt
    13201 ccaggccggc gatcaccgcg acttccagg
//
LOCUS       DAOMFA010000028        12768 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_462_length_12768_cov_5.427279, whole genome shotgun sequence.
ACCESSION   DAOMFA010000028 DAOMFA010000000
VERSION     DAOMFA010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 12768)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12768)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12768
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_462_length_12768_cov_5.427279"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            5..1096
                     /locus_tag="PKW44_02590"
     CDS             5..1096
                     /locus_tag="PKW44_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018077238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HQR50510.1"
                     /translation="MAFEATQHPNGKLLHSFRIIPDRGSWYEAQFDTNDLLYVYLDRK
                     KRRRKFLTTTLFRALNFLDHEGTKSKDKDADKNRGTDEEILKLFYEIEQLSVKEAEKL
                     YDLQNKVLIKDATDEEKGLVVARAFEPLSKAVVEQIAELGVTKIKVVDTSVDDGIVIN
                     LPQRMLFRSVGGKIQSAYPVALGKPDWPTPKGKFVILTREKDKTWLVPPSIQQEMLLE
                     GKEVLERVPPGPDNPLGRYWLGLSLPGIGIHGTLAPQSIYRFASHGCIRLHPDDVEQL
                     YRVVEKDEAGSLIYQPVLLFEAVDGRIFLEVHRDIYKKGVDANRMARELADAAAVTSR
                     VDWGRAASVIAAHEGLARDVTMDANREMP"
     gene            1093..1674
                     /locus_tag="PKW44_02595"
     CDS             1093..1674
                     /locus_tag="PKW44_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018077239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HQR50511.1"
                     /translation="MKQNSENMTATEKSVTARRAFLKAGAALLAAGVSGRAFATAPSV
                     GALERKLDFYNTHTDEQLSVVYWADGAYQPEGLTDIYRVLRDYRTGDVSPISSRLLDL
                     LHTLSTSLDARQPFHVISGYRSPTTNAMLAEKSNGVAKHSLHMEGLAIDIRLPGRELA
                     DVRRAAIALGYGGVGYYPASDFVHVDVGRVRTW"
     gene            1674..2351
                     /locus_tag="PKW44_02600"
     CDS             1674..2351
                     /locus_tag="PKW44_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HQR50512.1"
                     /translation="MQHAADDLSWLSVFPDLAGLETSAKDKLRQHVRIAEAPVGAVGY
                     REGSPCNAYVMRLAGRSRVQKISDSGREILLYRVGPGETCVLTTTCLLGHTHYPAETI
                     IEEAVRDAVVPAPVFHQLMLESEVFRRFVLSNYGDLISDLIVLVDEVAFRRLEARLAR
                     LLVEATQNSPAITSTHQQLAAELGSAREVVSRHLKEFERKGWLSLGRGQIEVVERPAL
                     EKLAQQT"
     gene            complement(2370..3326)
                     /locus_tag="PKW44_02605"
     CDS             complement(2370..3326)
                     /locus_tag="PKW44_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HQR50513.1"
                     /translation="MRILNMTLALLLSLYTGLALAGEFGDHCTTSLAAGLMVKSDCSQ
                     HSLFEGKTYCFGTEASKMAFDNAGNKQEIIDKAATFYAKNAGAERVKISQADAVEQIQ
                     SKACDLSNKDAGYLELDGMDLRHCKMVNVSFFGAYLRGANLSGANLQRAYLNLARLEN
                     ADLSGADLTDAIIFQAIFDKTNFKGANLTNARVIGTLGNVDMSGATIRNGRFGLDIGN
                     QPMGQMKFDSVGGNFANANFEGADLNIAAFPFGNLTGANLRNTNMHRADLSQADLTGA
                     DLTNANLDSAEVDGANFKGVKGLGTVKGFATVKGKCRDCAAK"
     gene            3565..4179
                     /locus_tag="PKW44_02610"
     CDS             3565..4179
                     /locus_tag="PKW44_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019897856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HQR50514.1"
                     /translation="MNAYKFGTVLIVASTVAMPAAADDVYYDTARVLSVTPQTERVNS
                     PRQECRTEYIQESYRSGDRDIAGSIIGGVAGGLLGSQFGKGNGRVAAAAVGAATGAVV
                     GDRIDNSDRQSYASRPVERCSTVDNWQTVNRGYLVTYRFDDRDYTTVMAYDPGRTMRV
                     RVSIAPEEGERVSYLEPAYRRDAGLHRGWDKYHDKDHKHGRWDD"
     gene            complement(4250..5944)
                     /locus_tag="PKW44_02615"
     CDS             complement(4250..5944)
                     /locus_tag="PKW44_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019899043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HQR50515.1"
                     /translation="MNSIFFLILRRMRAPLITLIVSYTIAILGFTLAPGVDAQGNPWQ
                     MSLFEAFYVVSYTATTIGFGEIPYAFSQAQRLWMTFSIYFTVIAWFYAIGKIIALMQD
                     PGLRHAITVARFARAVRRLGEPFYIVCGYGGTGSLLVEALDRLGIRAVTVDISQERIN
                     ELELQDYQAPVPGLCMDARLPDSMIQAGILHPLCGGVAALTDDDQANLAIAVTVKLLK
                     PELKVLCRAENQETATNMASFGTDHIINPYELFGEHLAMEVHALGTYLLHEWLTGVPG
                     SALPAPACPPIGKWIVCGFGRFGKAVVSNLQREHMQTVVVEADPQLTHCDDCIIGSGA
                     QARTLIEAGIEDAIGIVAGTDNDVNNLSIVMTALELKPDLFVVIRKNKRHNAPLFQKF
                     SADITMQPSDIIAHECLAHLVTPVLAQFLALARSQSNAWANALIGRLVETVGENVPET
                     WRVTLSGELAPAVINLLGHGKTVLLETLTSDPANRETRLPLVPLLLLRGGETILLPEP
                     SQSLEKDDRILFCGKNSAKSAQSLLLTNRKVLTYVRDGSETPDGVVWRWIASRARK"
     gene            complement(5958..6338)
                     /locus_tag="PKW44_02620"
     CDS             complement(5958..6338)
                     /locus_tag="PKW44_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6394 family protein"
                     /protein_id="HQR50516.1"
                     /translation="MNLEKVVFGFFILLAMTLNFGFFIGDMADPEFHHPYELFAAIVV
                     NLVATVLKFGDRTQVGAIHLSTSLVADLQLITAAVVWVAYSGLGTDIPAGIMVSIVSL
                     SGGAFLANVTSVVLLIIETIMLRR"
     gene            complement(6413..7933)
                     /locus_tag="PKW44_02625"
     CDS             complement(6413..7933)
                     /locus_tag="PKW44_02625"
                     /inference="COORDINATES: protein motif:HMM:NF017085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HQR50517.1"
                     /translation="MTHEVELKLRIAAQDIPHLRRHPAIKAALVGESITRKLVSIYFD
                     TPQLALLDAGISLRARYMAGAWYQAVKGSGKSLAGLHQRKEWEDQIASGQPDFSKITD
                     PALTAIFDNEDLRAALSPIFRTEVKRTEWHLAFDKGDQIELALDVGDLVVGERRETIS
                     EIELELKGGNTGRLFDFALELQQTIPLELENISKAQRGYGYYRPQPPTIIKAQPTRLQ
                     PEMSAHEALKQIAWECLTHLQGNQDMVLHGEDIEGVHQMRVALRRLRSALNIFSSVTS
                     KQNCAAIVGELRWITGVLGLARDLDVFTTQTLPPVLQQLPAHPGLHQLNDKAAAARLQ
                     AYVEVRVALSSQRYHRLLLNLGAWLENERWRDAGSVECSVLEIAQTMLAKRHKQFKKH
                     GQRLMHAHPEERHVTRIAAKKLRYAAEFFTGLFPVASSHDFLRPLAKLLDVLGILNDI
                     AVTENLIRRLIGGHPNRPLDESLHLFTGWNACNAMHRLEGMERAWSAFAGQKPFWV"
     gene            complement(7930..8556)
                     /locus_tag="PKW44_02630"
     CDS             complement(7930..8556)
                     /locus_tag="PKW44_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HQR50518.1"
                     /translation="MRTILIANSKGGCGKTTVATNLAGYFASRGRRVFLSDMDRQQSS
                     LSWLKRRSKKLPRIHGIDGRSHNGSDPGAEWTVIDSPAGFHGEKLSDAIKQADWIIVP
                     MQPSAFDIGATSDFLEALREEKAVRKERAFVAMVGTRVDPRTRAAATLNEFLQDTGFP
                     VLTCLRNAQVYIQAAEQGTSLFDFRPSLVPRDLEQWSPLLQWLVATDK"
     gene            complement(8603..9055)
                     /gene="sixA"
                     /locus_tag="PKW44_02635"
     CDS             complement(8603..9055)
                     /gene="sixA"
                     /locus_tag="PKW44_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HQR50519.1"
                     /translation="MDLILWRHAEAEDGIPDMERALTAKGRKQAKKMAGWLKSRMPED
                     IRILVSPAKRTQQTAAALGMDFNTLQEIAPGASAHSLLKATHWPHGEQSVLIVGHQPS
                     LGEVASLLLAGQDTGFSVKKGAVWWFNGRTRLGTSESLLLAVVAPDLL"
     gene            9111..10085
                     /locus_tag="PKW44_02640"
     CDS             9111..10085
                     /locus_tag="PKW44_02640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR50520.1"
                     /translation="MQKFSPECHLVVNSRLPGMGDVGGRAPALENLLAVGCRDVQERN
                     VYAWLSKWFGFPAAPLAALSLLGEGLDPDAYYWLHADPVHFQLQRDSFLLADPDLARM
                     DAEESRLLIDALNRHFEADGLLLLAPHPQRWYLRVNKVPAITTHPLQQVIGRNVEPLL
                     PQGADAARWRGWLNEVQMLLHDHPVNRVREDRGQLPINSVWLHGGGAYTAGVMTDISQ
                     VWADDVLVGGLALASGLQLRPSPASATAWLEQTPVSGAHLIILDQPGLSQLDQGWCSP
                     LLDALRQRRVSSLTLHFNEVDKVISYRIKRRDLWKFWRRRQALGGWIG"
     gene            10078..11769
                     /gene="recJ"
                     /locus_tag="PKW44_02645"
     CDS             10078..11769
                     /gene="recJ"
                     /locus_tag="PKW44_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HQR50521.1"
                     /translation="MVSIVTRDYPAQAAEDLQRAGCHPVLAHVLAARGITSMQQLECA
                     PAGLIPPQRLTHAERMAGILADAIAQQKRLLIVADYDADGATACAVGVRGLGMFGAQV
                     DYLVPNRFEFGYGLTPEIVALAAQRQPDFIITVDNGIASVEGVRAANAHGIAVLVTDH
                     HLPGDELPDAACIINPNQPGCSFPSKHLAGVGVMFYLLLALRAELRKRGAYASAPEPN
                     LTELLDLVALGTVADLVRLDENNRILVEQGLRRIRAGKASSGINALLRVAGRDPRRVC
                     AQDMGFGVAPRLNAAGRLQDMALGIDCLLSSDEDEALRMAQRLDTLNRERRDIEADMH
                     ESALAALEDFDPGERYSLSLYQDDWHQGVIGILASRLKERFHRPTIVFAPAGEGMLKG
                     SGRSITGLHLRDALDLVSKRHPGLLLRFGGHAMAAGLTIAGHDLERFTEALESVVRDL
                     LTPADLECVIETDGAIDAADFGLELAEVLEHQVWGQGFAAPEFDDRFTVTEQRIVGER
                     HLKLKLEKSGRPVNAILFNCSDLLPAKIRAVYRPQVNDYNGNRSLQLVVRYWEGC"
     gene            11912..12739
                     /locus_tag="PKW44_02650"
     CDS             11912..12739
                     /locus_tag="PKW44_02650"
                     /inference="COORDINATES: protein motif:HMM:NF014066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HQR50522.1"
                     /translation="MRNARDHGFLWRVSKDGRTSYLYGTMHAARMEWMFPGPRVSQAL
                     QASAVMALELDMLDPEVQRRMTEGMAVLKGAPLPEALARRMEKLMRAECIPPEQAGGM
                     IPEMQLATLSIVAGRRDGIDPAYAIDLVLAGHGHAAGMRVVSLETPEGQLGMLQMETS
                     QDTIALVERDLEEIENGRARVMLNRVASVWSDSDGATMQRYSEWCECMDSEVDRKLMK
                     RLLDDRNPALAQRIDALHGEGKSVFAAVGSLHMFGPQGIPALLSKRGYRVERVDFSR"
BASE COUNT         2463 a         3572 c         4087 g         2646 t
ORIGIN      
        1 gggtattgcc ttcgaggcca cgcagcatcc gaacggcaag ctgctccatt ccttccgtat
       61 tattcccgac cgcggttcgt ggtacgaggc gcagtttgac acgaacgatt tgctttatgt
      121 ttatctcgac cgcaagaagc gccgccggaa atttttgacg acaaccctct tccgcgcgct
      181 caacttcctg gatcacgaag gcaccaagag caaggacaag gacgccgaca aaaatcgcgg
      241 cacggatgag gaaatcctca agctgttcta cgagatcgaa caattatcgg tcaaggaagc
      301 cgagaagctt tatgacctgc aaaacaaagt cctgattaaa gacgcgaccg acgaggaaaa
      361 ggggctggtc gtggcgcgtg cttttgaacc gttgtccaag gcggtggtcg agcagatcgc
      421 cgaactcggc gtcaccaaga tcaaagtggt ggatacttcc gttgatgatg gcatcgtcat
      481 caatcttccg cagcgcatgc tgttccgctc ggtcggcgga aagattcaat ccgcctatcc
      541 ggtggccctg ggcaagccgg attggcctac gccaaaggga aaatttgtta ttctgacccg
      601 ggaaaaggac aagacctggc tggtgccccc gtccatccag caggaaatgc tgctcgaagg
      661 caaggaggtg ctggagaggg tgccgcccgg cccggataac ccgctggggc gttactggct
      721 ggggctgagc ctgccgggaa tcggtatcca tggtacgttg gccccgcaga gcatttatcg
      781 attcgccagt cacggctgta tccgcctgca cccggacgac gtggagcagt tgtatcgcgt
      841 agtggaaaaa gacgaggcgg gtagcctgat ctaccagccg gtgttgctgt tcgaagctgt
      901 ggatggcagg atattccttg aagtccaccg ggatatctac aaaaaaggcg tggatgctaa
      961 tagaatggcg agggaacttg ccgatgcggc ggctgtcact agcagggttg attggggacg
     1021 ggcagcatcc gttatcgccg cgcacgaggg tttggccaga gatgtgacga tggatgccaa
     1081 cagggagatg ccatgaagca gaacagcgaa aacatgactg ccacggaaaa atcggtaacg
     1141 gcgcggcgcg ccttcctcaa ggcgggtgcc gcattgctgg cagccggggt gtctggcagg
     1201 gcatttgcca cggcaccatc cgtgggggcc ctcgaacgca aactggattt ttacaacacc
     1261 cataccgacg agcagctgag cgtcgtgtac tgggcggatg gcgcttacca gcctgaaggg
     1321 ctgacggata tttaccgcgt tctgcgtgac tatcgtaccg gcgatgtcag ccccatcagt
     1381 agtcgcttgc ttgacctgtt gcacacctta tcgacctcgc tcgacgccag gcaacccttc
     1441 cacgtcattt ccggctatcg ctccccaacc accaacgcca tgctggcgga aaaaagcaat
     1501 ggcgtagcca agcacagcct gcacatggaa ggacttgcca tcgacatccg gctgcctggc
     1561 cgcgaactgg ccgacgtgcg ccgcgccgct attgccttgg ggtatggcgg cgtgggctat
     1621 tatcccgcct cggatttcgt gcatgtggat gtcggtcgcg tgcgcacctg gtgatgcaac
     1681 atgccgctga tgatctgagc tggctttcgg ttttccctga ccttgctgga ttagaaactt
     1741 ctgcaaaaga caagctgcgt cagcatgttc gtatcgccga ggcgccggtg ggcgcggtcg
     1801 gctatcgtga aggctcgccc tgcaacgctt atgtgatgcg gctggcaggg cgctcgcggg
     1861 tgcagaagat ttccgacagc gggcgcgaaa tcctgctgta ccgcgtcggt cccggcgaaa
     1921 cctgtgtgct cactaccacc tgtctgcttg gccataccca ctacccggcg gaaaccatta
     1981 tcgaggaagc tgtacgcgac gccgtggtgc cggctccggt gttccaccag ttgatgctgg
     2041 aatccgaggt gttccgccgc ttcgtgctat ccaactatgg cgaccttatc agcgacctca
     2101 tcgtgctagt cgatgaagtg gcgttccgtc ggttggaagc gcgactggcc cgcctgttgg
     2161 ttgaagccac gcaaaacagc ccagcgatta cctctaccca tcagcaactg gccgccgaac
     2221 tcggtagcgc gcgcgaggtg gtcagtcgcc atctcaagga gtttgagcgc aagggctggc
     2281 tgagtctggg gcgcggacag atcgaggtgg ttgagcgtcc tgctctggaa aagctggcgc
     2341 agcagacctg atttgccggg cgtggagtgt tactttgccg cgcaatcgcg gcattttcct
     2401 ttcaccgtgg caaatccctt gaccgtaccc aatcccttca cgcccttgaa attagcaccg
     2461 tccacttccg cgctatccag gttggcgttg gtcaggtcgg caccggtcag gtcggcctgg
     2521 ctgagatcgg cgcgatgcat attggtgttg cgcaaattgg ccccggtcag attgccgaag
     2581 gggaaggcgg cgatgttgag gtcggcgcct tcaaaattgg cgttggcaaa gttgccgccg
     2641 accgagtcga acttcatctg ccccatcggc tggttgccga tatccaggcc gaagcggccg
     2701 ttcctgatgg tggctccgct catgtcgacg ttgcccaaag tgccgatgac gcgcgcgttg
     2761 gtcaggttgg cgcccttgaa attcgtcttg tcgaaaatgg cctggaagat gatggcgtcg
     2821 gtcaggtcag cgccggagag gtcggcattt tccagccgtg ccagattgag gtaggcgcgc
     2881 tgcaggttgg cgccgcttaa attcgctccg cgcagatagg cgccgaaaaa gctgacgttg
     2941 accatcttgc agtggcgcaa atccatgcca tccagttcca gataaccggc atccttgttg
     3001 gagaggtcgc aggccttgct ctggatttgc tcgactgcgt cggcctggct gattttgacg
     3061 cgctcggccc cggcattttt ggcataaaag gtggccgcct tgtcgatgat ctcctgtttg
     3121 ttcccggcat tatcgaaagc cattttcgag gcctcggtgc cgaagcaata ggttttccct
     3181 tcaaacaagc tatgttgcga acaatcgctc ttgaccatca ggcctgccgc cagactggtc
     3241 gtacagtggt cgccaaattc gccggctagc gctaaaccgg tatacaggct gagcagcaat
     3301 gccaaggtca tattcaggat acgcatcaca tttctcctca caatcgttga aaatcggtgc
     3361 gcttcaagcg ccgtgacgca actattggtt taattgcgag gggcgtctgt aaccttagtc
     3421 acatagttga gtaaaaaatt caagtttatc gtttttgtgc ggaccgaccg gtcggtcaac
     3481 caaatcaaaa tctcccgcgt tattggctga ccagtatgaa atcactggta gcaaaagatc
     3541 aaaacaacgc aaggagattg aaaagtgaac gcatacaaat ttggtacagt cctgatcgtg
     3601 gcaagcactg ttgcaatgcc ggctgcggca gatgacgtgt attacgatac cgcacgcgtg
     3661 ctttccgtta ccccgcagac cgagcgtgtc aacagcccgc gtcaggagtg ccgtaccgaa
     3721 tacatacagg aatcctaccg ttccggtgat cgcgacatcg caggttctat catcggcggc
     3781 gttgccggtg gcctgctcgg cagccagttc ggcaagggta atggccgtgt tgccgccgcc
     3841 gccgtgggtg ccgctaccgg cgctgtcgtc ggcgaccgta tcgataacag tgacaggcaa
     3901 tcctatgcca gccgcccggt ggaacgttgc agcacggtgg acaactggca gaccgtcaat
     3961 cgcggctatc tggtgaccta ccgattcgat gatcgcgact acaccaccgt gatggcttac
     4021 gatcctggcc gaaccatgcg ggtgcgtgtt tcaatcgccc ccgaggaggg agagcgcgtc
     4081 agctatctgg aaccggccta ccgccgagat gccggattgc accgtggttg ggataagtat
     4141 catgacaaag accataaaca tgggcgttgg gatgactgaa cgcccactgg aagtgcggtc
     4201 ctgagcaatg ccgccggcgg atgccggcgg ttttttatta tccttgcgcc tacttgcgtg
     4261 cccgggaggc tatccatcgc cagactaccc catccggcgt ttctgagcca tcgcgcacat
     4321 acgtcagcac cttgcggttg gtgagcagca atgactgggc ggattttgcg ctgtttttgc
     4381 cacagaacag aatgcggtca tccttttcaa gggattggga aggttccggc agcaggatgg
     4441 tttcgccgcc gcgcagcaga agcaagggca cgagcggcaa acgcgtttcg cggttggccg
     4501 ggtcgctggt caaggtttcc agcagcacgg ttttgccgtg ccctagaagg ttgatgaccg
     4561 ccggtgcgag ttcgccggag agcgtgactc tccaggtttc cggcacgttt tcccctacgg
     4621 tttcgaccag ccggccgatc agtgcattgg cccaggcgtt gctctggctg cgtgcgagag
     4681 cgagaaactg tgccagcacg ggggtgacta gatgcgccag gcattcgtgc gcgatgatgt
     4741 cgctcggttg catggtgatg tcggcgctga atttctggaa tagcggggcg ttgtggcgtt
     4801 tgttcttgcg gatgacgacg aacaggtcgg gcttgagttc cagtgcggtc atgacgatgg
     4861 agagattgtt gacgtcgttg tcggtgccgg ccacgatacc gatggcatct tcaatacccg
     4921 cttcgatcag cgtgcgtgct tgggcgccgc tgccgataat gcagtcgtcg caatgggtca
     4981 attgcgggtc agcctcgaca accacggttt gcatgtgctc gcgctgcagg tttgagacca
     5041 cggccttgcc gaagcggcca aagccgcaga cgatccattt gccgatgggg gggcaggcgg
     5101 gagcgggcag tgcgctgccc ggcacaccgg tcagccattc gtgcagcaga taggtgccga
     5161 gcgcatgcac ctccatagcc agatgctcgc cgaacagctc gtaggggttg atgatatggt
     5221 cggtgccgaa agaagccatg ttggtggcgg tctcctgatt ttccgcacgg cacagcacct
     5281 tcagttccgg tttaagcagt ttgaccgtga cggcaatagc gagattggcc tggtcgtcat
     5341 ccgtgagcgc ggcgacgcct ccgcacagcg gatgcaggat gccggcctgt atcatgctgt
     5401 cgggcagtct ggcatccata caaagaccgg gcacaggagc ctggtaatcc tgcaattcca
     5461 gttcattgat gcgttcctgg gagatatcta cggtcacggc acggatgccc agacgatcga
     5521 gcgcttccac cagcaggctg ccagtgccgc catagccgca cacgatgtaa aagggttcgc
     5581 ccagacgccg cactgcccgt gcgaagcgtg cgacggtgat ggcatggcgc agtcccggat
     5641 cctgcatcag ggcgatgatc ttgccgatag catagaacca ggcgatgacg gtgaagtaga
     5701 tggaaaacgt catccatagc cgctgcgcct ggctgaaagc gtacgggatt tcgccaaagc
     5761 cgatggtggt ggcggtgtag ctgacaacgt aaaacgcctc gaacaggctc atctgccagg
     5821 ggttgccttg cgcgtccacg ccgggtgcca gggtaaaacc taaaatcgcg atcgtgtagc
     5881 tgacaatcag cgtaatcagc ggcgcgcgca tgcgccgcag gataagaaag aaaatgctgt
     5941 tcacgggatg ccggaaatta acggcgcagc atgatagttt cgataatcag cagtaccacg
     6001 gaagtgacgt tggccaggaa ggcgccgccc gatagcgaaa cgatgctgac catgatgccg
     6061 gccggaatgt ccgttcccaa gccgctgtat gcgacccaga ccactgcggc ggtaatcagt
     6121 tgcaggtccg cgacgaggct ggtggaaagg tggatggcgc cgacctgcgt gcggtcgccg
     6181 aatttgagca cggtcgcaac cagattgacg acgatagcgg caaaaagctc ataaggatgg
     6241 tggaactccg gatcggccat gtcgccgatg aaaaaaccga aattgagcgt catcgccagc
     6301 aggatgaaga agccgaaaac tactttttcc aggttcatga tgactccatg gcaagagatt
     6361 agacagcgaa agtgtagttg aattatcggg gcgcagcacc ctggttccgt gttcagaccc
     6421 agaatggctt ctgtcctgcg aaggcgctcc aggcccgttc catgccttcc agccggtgca
     6481 tggcgttgca ggcattccag ccggtgaata ggtgcagaga ttcgtcgagt gggcggttgg
     6541 ggtggccgcc gatgaggcgg cggatgaggt tttcggtgac ggcgatgtcg ttgaggatac
     6601 cgaggacatc cagcagtttt gccagcgggc gcaggaagtc atgagaactc gctacgggga
     6661 agagcccggt gaagaactcg gcggcatagc gcagtttctt ggcggcgatg cgcgtgacgt
     6721 ggcgttcttc cgggtgtgcg tgcatcagcc gctggccgtg cttcttgaac tgcttgtggc
     6781 gcttggcgag catggtctga gcgatttcca gaacgctgca ttcaacgctt ccggcatcgc
     6841 gccagcgttc gttttccagc caggcgccga ggttgagcaa cagccggtgg tagcgctgcg
     6901 aggaaagcgc gacgcggacc tccacatacg cttgcaggcg cgcggcggcg gccttgtcat
     6961 tgagctggtg caggccaggg tgggcaggca gttgttgcag cacgggcggc agggtctgcg
     7021 tggtgaatac gtcgaggtca cgcgccaggc cgagcacgcc ggtgatccag cgcagttcgc
     7081 cgacgatggc ggcgcagttt tgcttgctcg tcacgctgct gaatatgttg agggcggaac
     7141 gcagccggcg cagggcgacg cgcatctggt gcacgccttc gatatcctcg ccgtgtagca
     7201 ccatgtcctg gttgccttgc aggtgggtga ggcattccca ggcgatctgc ttcagcgcct
     7261 catgggcgct catttcgggt tgaagccggg tgggctgcgc cttgatgatg gtgggtggtt
     7321 gcgggcggta atagccatag ccgcgctggg ctttgctgat gttttcaagt tccagtggaa
     7381 tggtttgctg cagttccagc gcgaagtcaa acaggcgacc ggtattgccg cctttgagtt
     7441 ccagctcgat ttcgctgatc gtctcgcgcc gctccccgac caccagatcg cccacgtcga
     7501 gcgccagctc gatctggtca cccttgtcga aggcaagatg ccattcggtg cgcttgacct
     7561 cggtgcggaa aataggcgag agggcggcgc gcaagtcttc gttatcgaaa atcgcagtca
     7621 gtgccgggtc ggtgatcttg ctgaaatccg gctggccgga ggcaatctgg tcttcccatt
     7681 ccttgcgctg gtgcaagccg gcgagcgact taccggagcc cttgaccgcc tggtaccagg
     7741 cgcctgccat gtatcgtgcg cgcaggctga tgccggcatc gagcagggcg agttgcgggg
     7801 tgtcgaaata gatgctgacc agcttgcgag tgatggactc gccaaccagc gcggccttga
     7861 tggcgggatg ccgccggagg tgcggaatat cctgcgcggc gatgcgcagc tttaattcga
     7921 cctcatgcgt catttatccg tagccaccag ccattgcagc agcggcgacc actgctccag
     7981 atcgcgcggt accaatgaag ggcggaaatc aaacaggctg gtgccttgtt cggctgcctg
     8041 tatgtagact tgggcgttgc gcaggcaggt cagcaccggg aagccggtgt cctgcagaaa
     8101 ctcgttaagc gtggcggcgg cgcgggtacg gggatccacg cgcgtgccga ccatagcgac
     8161 gaaggcgcgt tccttgcgca ccgctttttc ctcgcgcagc gcttccagga agtcgctggt
     8221 ggcgccgatg tcgaaagccg agggctgcat cggcacgatg atccagtcgg cctgcttgat
     8281 ggcgtcggac agcttttcgc cgtgaaagcc ggcgggggag tcgatcactg tccattccgc
     8341 gccggggtcg ctgccgttgt ggctgcgccc gtcgatgccg tgaatgcgcg gcagcttctt
     8401 ggagcggcgc ttgagccagc ttagcgagga ttgctggcga tccatatccg acagaaagac
     8461 gcgccggccg cgcgaggcga aatagccggc aagattggtg gcgacagtgg tcttgccgca
     8521 tccgcccttg gaattggcaa tcagaatggt acgcatggct tgggctccgg caggcggttg
     8581 attttaaaga cgttgcagaa tatcacagta aatccggcgc aactaccgct aacaacaggc
     8641 tctcgctggt gccgaggcgt gtccgtccgt tgaaccacca cacggcgcct ttcttgacgc
     8701 tgaaaccggt atcctgccct gccagcagca gtgaggcaac ctcgcccagg gacggctggt
     8761 ggccgacgat cagcacgctt tgctctccgt gcggccagtg ggtagccttg agcagagagt
     8821 gtgcggaagc gccgggggcg atctcttgca gcgtgttaaa atccatgcca agtgcagcgg
     8881 cggtttgctg ggtgcgtttg gccgggctga ccaggatgcg gatgtcctcc ggcatgcggg
     8941 atttaagcca gccggccatt tttttcgcct gcttgcggcc tttggcggta agggcgcgtt
     9001 ccatgtcggg aataccgtct tcggcttcgg cgtggcgcca gagtatcaag tccatgagga
     9061 tgctcctgaa gaattccttg tattttatca ggatattagg ttcttaaaac atgcagaagt
     9121 tttctcccga atgccatctt gtggtcaaca gccggcttcc cggcatgggc gatgtcggcg
     9181 gcagagcgcc tgcgctggaa aatctgctgg cggttggatg tcgtgacgtg caggagcgta
     9241 atgtatacgc ctggctttct aaatggttcg gtttccccgc agcgccgctg gcggcgttgt
     9301 cgctgctggg ggaggggctg gatccggacg catattactg gctgcatgct gatccagtac
     9361 atttccagtt gcagcgcgac agttttctgt tggctgatcc cgacctggca aggatggatg
     9421 cagaggaatc caggctgctg atcgacgcgc tgaaccggca cttcgaggcg gatgggctgc
     9481 tattgctcgc ccctcatccg cagcgctggt atctgcgggt gaataaggtg cctgccatta
     9541 ctacacaccc gttgcagcag gtgatcggcc gcaatgtcga gcctttgctg ccgcagggtg
     9601 cggatgccgc ccgctggcgc ggctggctga acgaggtgca gatgctgttg catgatcacc
     9661 cggtcaaccg ggtccgggaa gatcgtggtc agctgccgat aaacagcgtc tggctgcatg
     9721 gtggcggggc ttatactgct ggcgtcatga cggacatcag ccaggtctgg gccgatgacg
     9781 tgctggtagg cggcctcgcc ttggccagcg gcttgcagct ccggccgtcg ccagcttctg
     9841 ccactgcctg gctggaacag acgcccgtat caggtgcaca cttgatcatc ctcgatcagc
     9901 caggattgtc gcaactggat cagggctggt gcagcccttt gctggatgcc ttgcgccaga
     9961 ggcgtgtcag ttcgctgacg ttgcatttca atgaggttga caaggtgatc agttaccgca
    10021 tcaagcggcg cgatctgtgg aaattctggc gtcgccgcca agcgttggga ggctggattg
    10081 gttagcatcg tcacccgtga ttatccggca caggctgcag aagacttgca gcgcgccggc
    10141 tgtcatccgg tgctggcgca cgtgctggcg gcgcgcggca tcacttccat gcagcaattg
    10201 gagtgcgcgc cggcgggact gattcctccg caacggctga cccatgcgga gcgcatggcc
    10261 ggaatcctgg ctgatgccat cgcgcagcaa aaacgcctgc tgatcgtggc tgattacgat
    10321 gccgacggtg cgaccgcctg tgccgttggt gtgcgtgggc tgggcatgtt cggtgcgcaa
    10381 gtggactacc tggtgcccaa ccgcttcgaa ttcggctacg ggctgacgcc ggagatcgtg
    10441 gcgctggcgg cgcagcgcca gcctgatttc atcatcaccg tggacaacgg tattgccagc
    10501 gtcgagggcg tgcgtgctgc caacgcccat ggcatcgcgg tgctggtgac cgaccaccac
    10561 ctgccgggcg acgaactgcc ggacgccgct tgcatcatca atcccaacca gccgggatgc
    10621 agcttcccca gcaagcattt ggctggcgtg ggcgtgatgt tctacctgtt gctggctttg
    10681 cgcgccgagc tgcgcaagcg cggtgcttat gccagtgcgc cggagcctaa cctgacggaa
    10741 ctgctcgacc tggtggcgct gggtaccgtg gcagacctgg tgcggctgga cgagaacaac
    10801 cgcatcctgg tcgagcaagg cctgcgccgt atccgcgccg gcaaagccag ctccggaatc
    10861 aacgccctgc tgcgcgtggc ggggcgcgat ccgaggcgcg tctgcgcaca ggatatgggc
    10921 ttcggcgtgg cgccgcgcct caacgccgcc ggccgcttgc aggatatggc gctggggatt
    10981 gactgtctgt tgagcagcga tgaagacgaa gctctgcgta tggcgcagcg gttggatacg
    11041 ctcaaccgcg agcgacgcga tatcgaggcg gatatgcacg aatccgcact ggcggcgctg
    11101 gaggattttg accctggcga gcgatacagc ctcagcctgt atcaggacga ctggcaccag
    11161 ggcgtgatcg gcatcctggc ctcgcgcctg aaggagcgct tccaccgtcc caccattgtg
    11221 ttcgccccgg ccggcgaagg catgctgaaa ggctctggcc gttccattac cggactgcac
    11281 ctgcgcgatg cactggacct ggtgtccaag cgacaccccg gcttgctgct gcgctttggc
    11341 ggccatgcca tggcggctgg gctgacgatt gccgggcacg atctggaacg gtttaccgag
    11401 gcgctcgaat ccgtggtacg tgacctgttg acgccggccg atctggagtg tgtgatcgag
    11461 accgatggcg ccattgatgc tgcggacttc ggtctggaac tggcagaggt gctggagcat
    11521 caggtatggg ggcaaggttt tgccgcacca gagttcgatg accggtttac cgtcaccgag
    11581 cagcgcattg tcggtgagcg ccaccttaaa ctaaaacttg aaaaatccgg ccgtcctgtc
    11641 aatgccatcc tgtttaactg tagcgatctg ctaccggcaa aaatccgtgc cgtataccgt
    11701 ccacaagtca acgattacaa tggtaaccgc agcctgcaat tggtggtgcg gtactgggaa
    11761 gggtgctgat ctgggtaacg ctggtatgaa aaaaatattt gttgcccttt ttctgttact
    11821 gacagggcta tcttgccatg catgggccga ggccatgatc gattgcccgc cccaagcggc
    11881 agcgcccacc gcggaacagg ctcgcgccgc tatgcgcaac gcgcgcgatc acggttttct
    11941 gtggcgtgtc agcaaggatg gccgtacttc ctacctttat ggcaccatgc atgctgccag
    12001 gatggagtgg atgtttcccg gtccgcgggt gtcgcaggct ttgcaggcga gtgccgtcat
    12061 ggcgctggaa ctcgacatgc tagacccaga agtccagcgg cgtatgacgg aaggcatggc
    12121 ggtgctcaag ggtgcgccgc taccagaggc gctggcgcgg cgcatggaaa agctgatgcg
    12181 agccgaatgc attccgccgg agcaggcggg cggcatgatc cccgagatgc agctcgccac
    12241 attgagtata gttgccggac ggcgcgatgg catcgacccg gcctacgcta ttgacctggt
    12301 gctggccggc cacggccatg cggccgggat gagggtggtt tcgctggaaa ccccggaggg
    12361 gcagctcggc atgctgcaaa tggaaacctc gcaagacacc atcgccctgg tcgaaaggga
    12421 cctggaagaa attgaaaatg gtcgtgcgcg tgtcatgctc aaccgtgtcg ccagcgtatg
    12481 gagcgattcc gatggtgcga cgatgcagcg ctacagcgaa tggtgcgagt gcatggatag
    12541 cgaggttgac cgcaaactga tgaagcgcct gctcgatgac cgcaacccgg ctctggcgca
    12601 acgtatcgac gcgctgcatg gcgagggaaa atcggtgttt gcggcggtcg gcagcctgca
    12661 catgttcggg ccgcaaggca ttcccgcgct tctgtccaag cgaggttacc gcgtggagcg
    12721 tgtggatttc agccgataac ggcttgttga tattcaactt cagggatt
//
LOCUS       DAOMFA010000029        12717 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_468_length_12717_cov_4.754304, whole genome shotgun sequence.
ACCESSION   DAOMFA010000029 DAOMFA010000000
VERSION     DAOMFA010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 12717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12717
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_468_length_12717_cov_4.754304"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..487)
                     /locus_tag="PKW44_02655"
     CDS             complement(<1..487)
                     /locus_tag="PKW44_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-Mn family superoxide dismutase"
                     /protein_id="HQR50523.1"
                     /translation="MEHTLPPLPYARNALVPHISEETFDYHYGKHHQTYVTNLNNLIK
                     GTEFENASLEDIVRKSSGGLYNNAAQTWNHTFFWNSLKPNGGGVPSGALGDAINTKWG
                     SFDEFKKAFQASAVGNFGSGWTWLVKKADGSVDIVNTGAAGTPLTTGDKALLTIDVWE
                     HA"
     gene            complement(575..1318)
                     /locus_tag="PKW44_02660"
     CDS             complement(575..1318)
                     /locus_tag="PKW44_02660"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009852252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HQR50524.1"
                     /translation="MLIQSHIVDLSTPTGVMRTYVHRPLAEGRYPAILFYSEIFQQTG
                     PIERAAKLMAGHGYAVLVPEVFHELNPIGTVLAYDDAGREKGNADKAAKPAESYDSDN
                     AVLVNYLKQQSWCSGKIGAMGFCIGGHLAFRASLQPEVKATACFYATDLHNNNIPNGP
                     RQHSMDRLGEIKGELLMIWGRQDNHIPAGGRAEVYRKLSDAGLTFTWHEFNGQHAFMR
                     DEGERYDPQLAVTGYQLALQLFSRTLAAI"
     gene            1612..1791
                     /locus_tag="PKW44_02665"
     CDS             1612..1791
                     /locus_tag="PKW44_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010565387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HQR50525.1"
                     /translation="MAEKLTQVNTRQTASPQDEKRIVEEVLAALRGLRFGSVEITVHE
                     GKVTFIERKEKVRLT"
     gene            1894..3501
                     /locus_tag="PKW44_02670"
     CDS             1894..3501
                     /locus_tag="PKW44_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HQR50526.1"
                     /translation="MQFKKKIIASLLISGGVLGAAGQASANDSSELEQLRALVQELDQ
                     KVRVLARKEELQEEDLEKQKKETPKIKASASGFGLESADGKNSIKLQGLLQSDYRAYN
                     NAYRDVRSRSDFRAGDLDNGLTGFSNTGDGWLIRRARPTVLGTLFGKYDYRFTEDFAG
                     GNATVVDAYVDARFDPAFQVQVGKYKSQFSLERLQGGGNIKFIERSYVANDIAPNRDL
                     GINLHGDVLDKKLSYSLAYNNGVQDGGNISTGTDFNGASEYTARLFTTPFKDQDSALS
                     GLGFGLAATHAHFSGQANLNYTDTSAANGSWNGLPSYVSDGQNTVFRYNTWTSGSTPG
                     AVADGTRFRLSPQANYYYGPFGLITEYVRISQGVSNYTGGSPAAGGTATDFVINKSSN
                     KTLNHDAWQIAGSWLLTGEDASFGKVKPKQDFDLDKGGWGAWELVARYHELYAPCKSK
                     AGTSYTQAAGAYSDLSKSVKSAHSYDLGVNWYLNEDVKVAVNYLHTTFEGGDIKPGTG
                     ANILANGSNVADRSDEQAIFARFQISY"
     gene            3531..4538
                     /locus_tag="PKW44_02675"
     CDS             3531..4538
                     /locus_tag="PKW44_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018229102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HQR50527.1"
                     /translation="MKTLYLTLSLLIAILALANPLNASAAELNLLNVSYDPTRELYQQ
                     FDAAFAKYWEAKTGNKVTIKASHGGSGKQARAIIDGLEANVATLALAYDVDQLYEKGK
                     LIPKDWQKRLPNNSSPYTSTIVFLVRKGNPKHIKDWDDLVKPGVAVITPNPKTSGGAR
                     WNFLAAWAYSLKQTNNDEAKAKEFVTKLFKNVPVLDSGARGSTTTFVERGIGDVLLAW
                     ENEAFLAKKELGPDKVDIVVPSISILAEPPVTIVDKVADKHKTRAVAQAYLEYLYSDE
                     GQDIAGKNFYRPTKPEVAAKYASQFPKVNLITIDEFGGWQKAQKTFFSDGGVFDQIYA
                     K"
     gene            4674..5033
                     /locus_tag="PKW44_02680"
     CDS             4674..5033
                     /locus_tag="PKW44_02680"
                     /inference="COORDINATES: protein motif:HMM:NF021593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2325 domain-containing protein"
                     /protein_id="HQR50528.1"
                     /translation="MTILVIGGDHIDKIRRELAEHGLHDVEHWPGRKPADARKAIPDR
                     IRLVVAVTDQLSHAMLYNTTLKAARLDLPIIYSRRSGHELREELAERYGKRPASALPK
                     GISSGWILPFSNIAISY"
     gene            5045..5260
                     /locus_tag="PKW44_02685"
     CDS             5045..5260
                     /locus_tag="PKW44_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HQR50529.1"
                     /translation="MAIHAINVRNQFKGRIKEIITGPVVSEVDIDTPSGIVTSVITTR
                     SVQELGLAPGSEVVALVKSTDVSLAKL"
     gene            5270..6327
                     /locus_tag="PKW44_02690"
                     /pseudo
     CDS             5270..6327
                     /locus_tag="PKW44_02690"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0043199 - sulfate binding [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
     gene            6364..>6824
                     /locus_tag="PKW44_02695"
     CDS             6364..>6824
                     /locus_tag="PKW44_02695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HQR50530.1"
                     /translation="MVEKIIKELKENLHDALFLDLDEFGLEVEGGEVISRFVGLKLHS
                     AFQPIRDVKQANQAIGYEALLRPAIGPDQINPPLAFDFADKQGRLVKLDRVARTLHML
                     NYLNLPQNPGLLFLNVHPKLLTSVNAHGKVFERVLHIHSVPTHQVVVEIQE"
     assembly_gap    6825..6924
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6925..7367
                     /locus_tag="PKW44_02700"
     CDS             <6925..7367
                     /locus_tag="PKW44_02700"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HQR50531.1"
                     /translation="LHIHSVPTHQVVVEIQESAVDIDKQLAEAIGNYRDRGYRIAIDG
                     FGGKQTNLKRLWQQPADYVKLDAGLIREAETSPKARGVLTKLVEIVQAVGAQAIIQGI
                     ESETQHRIALDAGATLLQGYDLGRPASSAEWLARKSGGTAKAAA"
     gene            7516..8358
                     /gene="cysT"
                     /locus_tag="PKW44_02705"
     CDS             7516..8358
                     /gene="cysT"
                     /locus_tag="PKW44_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020182705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HQR50532.1"
                     /translation="MNFTLHRRSSVLPGFTPALGYTILYLSLIVLIPLSAAFLKTTTL
                     TWGEFWAAVTAPPVLASYRLSFGAALLGAFINAFFGLLVAWVLVRYRFPGKRLIDALV
                     DLPFALPTAVAGIALTAVYSANGWLGSVLEPHGIKVAFTPLGVVVALTFIGLPFVVRT
                     VQPVLEDLEAETEEAAASLGANRFQTFQRVILPALWPALLTGFSLAFARAVGEYGSVI
                     FIAGNMPMVSEITPLLIITKLEQYDYAGATAIAVVMLVISFILLLIINGLQWWSTHRH
                     GVGR"
     gene            8361..9245
                     /gene="cysW"
                     /locus_tag="PKW44_02710"
     CDS             8361..9245
                     /gene="cysW"
                     /locus_tag="PKW44_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysW"
                     /protein_id="HQR50533.1"
                     /translation="MTIAVAIPANKYHSKVAQNRAIAEPAWLRWLLIGSALAFLGLFL
                     FVPLAAVFTEALRKGFTVYLAAFDEPDALSAIKLTFIAAAIAVPLNLVFGVTAAWAIA
                     KFEFKGKSVLITLIDLPFAVSPVIAGLVFVLIFGLQGWFGSWLSDHDIKVIFTIPGIV
                     LATIFVTFPFVARELIPLMQAQCKDEEEAAIVLGASGWQMFWRVTLPNIKWGLLYGVI
                     LCNARAMGEFGAVSVVSGHVRGLTNTLPLHVEILYNEYNYVAAFACASILAFLALVTL
                     ALKTLVEWQASRAATTLE"
     gene            9255..10313
                     /locus_tag="PKW44_02715"
     CDS             9255..10313
                     /locus_tag="PKW44_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter ATP-binding protein"
                     /protein_id="HQR50534.1"
                     /translation="MGIEIRNISKNFGSFSALNDITLDVPSGELVALLGPSGCGKTTL
                     LRIIAGLESPDNGGVLFNGEDTTQRHVRERQVGFVFQHYALFRHMTVFENVAFGLRVR
                     PKKTRPDDAEISRRVHKLLDLVQLDWLADRYPPQLSGGQRQRIALARALAVEPKVLLL
                     DEPFGALDAQVRKDLRRWLRRLHDELHITSVFVTHDQEEALEVADRVVVMNKGVVEQI
                     GTPSEVYDNPASPFVYHFLGNVNLFQSRVHAGFARVGDIELEAPEYHGAENAPAVAYV
                     RPHDIDIRREAGGDSAFKVVVNHIHPIGSVVRVELEREDESELIEAELTRERFRELDL
                     RTGDWVFVRPRNLRVFLQ"
     gene            complement(10480..10752)
                     /locus_tag="PKW44_02720"
     CDS             complement(10480..10752)
                     /locus_tag="PKW44_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HQR50535.1"
                     /translation="MNKSELIDAIAAEAGLTKADAGKALDAITNSVTAALKKGDNVTL
                     IGFGTFKVSSRAARTGRNPQTGKEIKIAARKAPAFTAGKALKDAVN"
     gene            complement(10854..11729)
                     /gene="xerC"
                     /locus_tag="PKW44_02725"
     CDS             complement(10854..11729)
                     /gene="xerC"
                     /locus_tag="PKW44_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020183505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HQR50536.1"
                     /translation="MDYLAAYLNHLAHERGLSALTCQNYGRDIRTLIELAGTTALQQL
                     QPPQIRRYVATLHGRGLGGKSIARMLSAWRGFYAYLGKRHGFVGNPCVGMRPPKSAKS
                     LPQALSPEQAGRLVEITDGDALAMRDHAMLELFYSSGLRLAELVSLEPSGLDLGNGTV
                     TVTGKGAKTRIVPVGSHAIAAIREWLPLRAMMKLDDEQALFVTRNGKRMTPRAVQYRF
                     KYWAIRQGVAGDVHPHILRHSFATHVLQSSGDLRAVQEMLGHANISTTQVYTHLDFQH
                     LAKVYDQAHPRARKK"
     gene            complement(11731..12393)
                     /locus_tag="PKW44_02730"
     CDS             complement(11731..12393)
                     /locus_tag="PKW44_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013440999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HQR50537.1"
                     /translation="MQLTEDQSTDYLRADPQFFERNAHLLTEIYLPSPHGSGTVSLAE
                     RQQLAQRDKIRVLEVKLAELLQFGEENDAIGDKVHRLCLAMLGSPSFDILMRLVVEHL
                     REDFKVPHVAIRLWAKPLNKEDAASEEFGALSEELRIWAQGLASPYCGHHPGPDLSDL
                     FGAANAPLKSYAFVALRGEEVFGLLAMASEDEQRFYPEMGTVYLKRIGELLSAALLRY
                     IQ"
     gene            complement(12407..>12717)
                     /locus_tag="PKW44_02735"
     CDS             complement(12407..>12717)
                     /locus_tag="PKW44_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HQR50538.1"
                     /translation="AEQGPLIERHQRFPQRVIEGFMHIVNPHRINLRVYVRGAGETLA
                     CGTGACAAAVAGIRLGKLASPVTVAMRGGELSISWAGEGQTVMMTGPAVTVFEGEIPL
                     "
BASE COUNT         2656 a         3814 c         3684 g         2463 t
ORIGIN      
        1 aggcgtgctc ccacacgtcg atggtcagca gcgccttgtc gccggtggtc agcggggtgc
       61 cggcagcgcc ggtgttgacg atgtccaccg agccgtcggc cttcttcacc agccaggtcc
      121 agccggagcc gaaattgccc acggcggaag cctggaatgc cttcttgaat tcatcgaacg
      181 aaccccattt ggtattgatg gcatccccca gcgcgccact gggcacacca ccgccattgg
      241 gcttcaggct gttccagaag aaggtgtggt tccaagtctg ggcagcgttg ttgtaaaggc
      301 cgccggaaga cttcctgacg atgtcttcca ggctggcgtt ctcgaactcg gtgcccttga
      361 tcaggttatt gaggttggtg acgtaggtct ggtgatgctt accgtagtgg tagtcgaaag
      421 tttcttcgga aatgtgcggc actagcgcgt tcctggcata gggcagcggg ggcaatgtgt
      481 gttccatgat gtggcctttc ttgaatgatg ggggatgaaa accagtggcg gtattttgcc
      541 acgaagtccg agtttcttaa tcaagtatta atggctaaat ggcagccagc gtccggctga
      601 acagttgcag cgccagctgg tagccggtca cggcgagctg cgggtcatag cgttcgcctt
      661 cgtcacgcat gaaggcgtgc tgtccgttga actcatgcca ggtgaaggtc aaaccggcat
      721 cggacagttt gcggtacacc tcggcgcggc cgccggcggg gatgtggttg tcctgcctgc
      781 cccagatcat cagcagttcg cccttgattt cgcccaggcg atccatgctg tgctggcgcg
      841 gcccgttggg gatgttgttg ttatgcaaat cggtagcata gaaacaagca gtggctttga
      901 cttccggctg cagcgaggca cggaacgcca ggtggccgcc gatgcagaag cccatggcac
      961 caatcttgcc ggaacaccac gactgctgtt tgagatagtt caccagcact gcgttgtcac
     1021 tgtcatagct ttccgccggc ttggccgcct tgtcggcatt gcctttctca cggccggcgt
     1081 cgtcgtaggc cagcacggtg ccgatcgggt tcagctcgtg gaacacctcc ggcaccagca
     1141 ccgcatagcc atgccctgcc atcagcttgg cggcgcgctc gatgggaccg gtctgctgga
     1201 agatttcgga atagaacagg atggcgggat agcgaccctc cgccagtggg cgatgcacgt
     1261 aggtgcgcat gacgccggta ggggtggaca ggtcaacaat atggctttga atcagcattg
     1321 cgtactcctg aaaattcgaa ccggacggct ctatctatat aaagttggta attgtaaaac
     1381 cgttcgcatc ttttcgcgca tcgggccatg catttttgta ttagccgccc ctcttacccg
     1441 gataagtcat cactttttcc agacaatttc cccgtcccac tacctgttgg ccttagaagc
     1501 caacagcctt taaataactt tatgttatga gttaataaaa acaaagtatt tcaaattatt
     1561 aacatggatt cgtatagtgc cagccattca gaacaggcag ttgaggcaga catggctgaa
     1621 aagctgacac aggtcaacac gcggcaaacc gcatcgccgc aggacgaaaa gcgcatcgtg
     1681 gaagaagtgc tggccgcgtt gcgcgggcta cgcttcggct ccgtcgagat caccgtacac
     1741 gaaggcaagg tgaccttcat cgagcgcaag gaaaaggtgc gcctgacctg aaacggaccc
     1801 ccctaccgga acaccggcgg gaaaaaacaa tcgatcaaat ctggcggacc gaccgggggg
     1861 acggaggaac gcaaaatcat catggagaac gtcatgcaat ttaaaaagaa aatcattgcc
     1921 tcgctgctga tcagcggcgg cgtgctaggt gcggcagggc aagcgtccgc caacgacagc
     1981 tccgaactgg agcagctgcg tgcgctggta caggaactgg accagaaagt cagggtactg
     2041 gcgcgcaagg aagaactgca agaagaggac cttgagaagc aaaagaaaga gactccaaaa
     2101 atcaaggcca gcgccagcgg cttcggcctg gaatccgccg acggcaagaa ctcgatcaag
     2161 ctgcagggct tgctacagtc cgattaccgc gcctacaaca atgcctaccg ggacgtccgt
     2221 agccgcagcg atttccgggc gggtgacctc gacaacggcc tgaccggttt ttccaatacc
     2281 ggcgacggct ggctgatccg ccgcgcgcgc cccacggtcc tcggcaccct gttcggcaaa
     2341 tacgactacc gcttcaccga ggactttgcc ggcggcaatg ccaccgtcgt cgacgcttac
     2401 gtcgatgccc gtttcgatcc ggcgttccag gtgcaagtgg gtaagtacaa atcgcaattc
     2461 agcctcgagc gcctgcaggg cggcggcaac atcaagttca ttgagcgcag ctatgtcgcc
     2521 aacgacattg cgccgaatcg cgacctcggc atcaacctgc acggtgacgt gctggacaaa
     2581 aagctgagct actccctcgc ctacaacaat ggcgtacagg acggcggcaa catcagcacc
     2641 ggcaccgact tcaacggcgc cagcgaatac acggcacgtc tgtttaccac gccgttcaag
     2701 gatcaggatt cggcattgag cgggctgggc ttcgggctgg ccgcgaccca cgcgcacttc
     2761 agcggccagg ccaacctgaa ctacaccgac acctccgccg cgaacggctc ctggaacggc
     2821 ctgcccagct acgtcagcga tggccagaac acggtgttcc gctacaacac ctggaccagc
     2881 ggcagcacgc ccggcgcggt tgccgacggc acgcgcttcc gcctctcgcc gcaggccaac
     2941 tactattacg gcccgttcgg cctgatcacg gaatacgtca ggatatcgca gggcgtcagc
     3001 aactacacgg gcggttctcc ggcggcaggg ggcacagcca ccgacttcgt gatcaacaag
     3061 agcagcaaca agacgctcaa tcacgatgca tggcaaatcg ccggctcctg gctgctgacc
     3121 ggggaagacg cctctttcgg caaggtcaag ccgaaacagg acttcgatct ggacaagggc
     3181 ggctggggcg cctgggaact ggtagcacgc taccacgagc tgtacgcacc ctgcaagagc
     3241 aaggcgggga cttcctatac gcaggctgcc ggggcttact ccgacctgtc gaagagcgtg
     3301 aagtctgcgc attcctacga tctcggcgtg aactggtatc tgaacgagga cgtcaaggtc
     3361 gcggtgaact acctgcatac caccttcgag ggcggggata ttaagcctgg aacgggcgct
     3421 aacatcctgg ccaatggctc gaacgttgcg gaccgtagtg acgagcaggc tattttcgcc
     3481 cgcttccaga tctcctacta acgcctcact cacattttta aggagcttta attaaaacac
     3541 tatatttaac cctgagtttg ttgatcgcca ttctggcgct ggccaacccg cttaacgcaa
     3601 gcgcagctga actcaacctg ctcaacgtgt cttatgaccc gacgcgcgag ctgtatcagc
     3661 aattcgatgc cgctttcgcc aaatactggg aagccaagac cggcaataag gtcacgatca
     3721 aggcctccca cggtggctcc ggcaagcagg cccgcgcgat tatcgatggc ctggaagcca
     3781 acgtcgccac cctggcgctg gcctacgacg tcgaccagtt gtacgagaag ggcaagctga
     3841 ttcccaagga ttggcagaag cgcctgccca acaacagttc gccctacacc tccaccatcg
     3901 tgttcctggt gcgcaagggc aaccccaagc acatcaagga ctgggacgat ctggtgaagc
     3961 cgggcgtggc ggtgatcacc cccaacccca agacctccgg cggtgcccgc tggaacttcc
     4021 tggcagcctg ggcgtattcg ctgaagcaaa ccaacaacga cgaggccaag gccaaggagt
     4081 tcgtgaccaa gctgttcaag aacgtgccgg tgctggattc aggcgcgcgc ggctccacca
     4141 ccactttcgt cgaacgcggt atcggcgacg tgctgctggc ctgggaaaac gaagccttcc
     4201 tggccaagaa ggaactgggg ccggacaagg tcgatatcgt ggtgccgtcg atttcgattc
     4261 tggccgaacc gccggtgacg atcgtggaca aggtggcgga caagcacaag acccgcgccg
     4321 tggcccaggc ctatctggaa tacctgtatt ccgacgaagg ccaggacatc gccggcaaga
     4381 acttctaccg cccgaccaag ccggaagtgg ccgcgaagta tgccagccag ttccccaagg
     4441 tcaacctgat taccatcgac gagttcggcg gttggcagaa agcgcagaag actttcttct
     4501 ccgacggcgg cgtgttcgac cagatctacg ccaagtaatc gtgcaacggg cggcggagat
     4561 cccgtcgccc gatcgctggg agcacaggaa aatcccatcc gccagcggtg ggcgggattt
     4621 cccggttagc ccgaaaaatt caggttgact tcaaaagagc aaggggcgca gcaatgacaa
     4681 tattggttat cggtggcgac cacatcgaca agattcgccg cgagctggcc gaacatggcc
     4741 tgcacgacgt ggaacactgg cccggccgca aaccggccga tgcaagaaaa gcgatcccgg
     4801 atcgcatcag gctggtggtg gcggtcaccg accagttgag ccatgcgatg ctctataaca
     4861 ccaccctcaa ggccgccagg ctggacctgc cgatcattta cagccgccgg tcggggcatg
     4921 aactgcgcga agaactggcg gaacgctatg gcaaacggcc agcgagcgcc ttgcccaaag
     4981 gaatcagttc cggctggatc ctgccgttca gcaatatcgc catcagctat tgaaaggaag
     5041 ttccatggcc atccatgcca tcaacgtgcg caaccagttc aagggacgca tcaaggaaat
     5101 catcaccgga ccggtcgtct cggaagtgga tatcgacacg ccatccggca tcgtcacctc
     5161 tgtcatcact acccgttcgg tgcaggaact cgggctggcg ccgggcagcg aagtggtggc
     5221 gctggtcaaa tccaccgacg tatccctggc gaaactttag gccagcggca tgacgatcca
     5281 ggccatcaac gcgcgcaacc agttcaaggg gcgcatcaag gaaatcgtat tcggcgacgt
     5341 ggtatcggaa gtggatatag aaacagccct gttgctgctc cccgtgcttg cccaggccga
     5401 caccacgctg ctcaacgtct cttacgacgt gacgcgcgag ttctacaagg attacaaccc
     5461 ggcgtttgcc aggcactgga aggccaagac cggcgaaacc gtgaccatca accagtcgca
     5521 cggcggctcc agcaagcagg cgcgctcggt ggcggatggc ctggaggccg acgttatcac
     5581 catgaaccag gccaacgaca tcgacatctt gtacgagcgc ggcaaactga ttccggccga
     5641 ctggcagaag cgcctgccca acgccagcgc cccaccacct cgaccaccgt gttcctggtg
     5701 cgcaagggca accccaagaa aatcaaggat tgggatggct tggtaaagcc aggcgtgtcg
     5761 gtcatcgtgc ccaatcccaa gacctcgggc aacggccgct atagctatct ggcggcatgg
     5821 ggtgcggtcg tgaaaaaagg cggcgacgaa aaggcggcgc gcgactatgt cacccgcctg
     5881 ttcaagaacg ttccggtgct ggataccggc gggcgcggcg ccaccaccac ctttgcccag
     5941 cgtggcatcg gcgacgtgct gctaaccttt gaaaacgagg tttacctgat tcagaaagag
     6001 ctaggtagcg accagtttga ggtggtttat ccctcgatca gcatccaggc ggaaaacccg
     6061 gtggcactgg tggacaaggt cgcggacaag cgcaagacgc gcgccgtggc gcaagcctat
     6121 ctggaatacc tgtacagcga ggaaggccag gaaatcgccg cacaacaccg cctgcggccg
     6181 cgcctggata gcgttgccgc caggcacgcc gcggatttcc gcaagctgga tctgtttacc
     6241 atcgatgaag tgttcggtgg ctggaagcaa gcgcaaaaag tgcatttcag cgatggcggc
     6301 acattcgatc agatttacgc caaataactc cggttcaatg gagcttggca taaggagcac
     6361 gacatggttg agaaaatcat caaggaattg aaagaaaatc tgcacgacgc gctgtttctc
     6421 gacctcgacg agttcggtct ggaggtcgaa ggtggcgaag tcatcagccg cttcgtcgga
     6481 ctcaaactgc acagcgcatt ccagcccatt cgtgacgtca agcaagcgaa tcaggccata
     6541 ggctatgagg cactgctgcg acccgccatc gggccggacc agatcaaccc gccgctcgcc
     6601 ttcgatttcg ccgacaagca ggggcgtctg gtcaaactcg atcgtgtcgc gcgcaccctg
     6661 cacatgctca attacctgaa cctgccgcaa aaccccggcc tgctgttcct caacgtacat
     6721 cccaagctgc tgaccagcgt caatgcccac ggcaaggtgt tcgagcgcgt actgcatatc
     6781 cattcggtgc ctacccacca ggtcgtggta gaaatccagg aaagnnnnnn nnnnnnnnnn
     6841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6901 nnnnnnnnnn nnnnnnnnnn nnnntactgc atatccattc ggtgcctacc caccaggtcg
     6961 tggtagaaat ccaggaaagt gctgtcgata tagacaagca gttggcggaa gccatcggca
     7021 actatcgcga ccgtggctac cgcattgcca tcgacggctt tggcggcaaa caaaccaacc
     7081 tgaaacgcct ttggcaacag ccggcggact acgtcaagct ggatgcaggg ctgatccgcg
     7141 aagccgaaac cagccccaag gcgcgtggcg tactaaccaa gctggtcgaa atcgtgcagg
     7201 ccgtgggcgc acaggccatc atccagggca tcgaaagcga aacccagcat cgcatcgcgc
     7261 tggatgcggg cgccaccctg ctgcaaggct acgacctcgg ccgtccggca tcctctgccg
     7321 agtggctggc ccgcaaatca ggaggcacag ccaaggccgc cgcttaacgt ttctccatct
     7381 cctccctcgg gcgtcttgta cggcaccctt tttttgtatc acaactttca gttattaatt
     7441 tattcaaaac tattatttct aaatataaat aaatctgact atgattgcgt cgccttacat
     7501 cactccagag ccaacatgaa tttcacactg caccggcgat cctccgtcct gcccggcttt
     7561 accccggcgc tgggctatac cattctctac ctcagcctga tcgtactgat cccgctgtcg
     7621 gcagcgtttc tcaagaccac gaccctgacc tggggggaat tctgggccgc cgtgactgcg
     7681 cctcccgtgc tggcgtccta ccgcctgtca ttcggcgccg ccctgctggg tgcgttcatc
     7741 aatgcctttt tcggcctgct ggtggcttgg gtgctggtgc gctaccgttt ccccggcaag
     7801 cgcttgatcg atgcgctggt ggatttgcca ttcgcactgc cgacggctgt ggccggcatc
     7861 gcgctgaccg ccgtctattc cgccaacggc tggctcggca gcgtgcttga gccgcacggc
     7921 atcaaggtgg cgttcacgcc gctgggcgtg gtggtggcgc tgacgttcat cggcctgccg
     7981 tttgtggtga gaaccgtgca gccggtgctg gaagatctgg aggccgaaac cgaagaggct
     8041 gccgccagtc ttggcgccaa ccgtttccag accttccagc gcgtcatcct gcctgcgcta
     8101 tggccggcat tgctgaccgg cttctcgctg gcctttgccc gcgccgtggg cgaatacggc
     8161 tcggtcattt tcatcgccgg caacatgccc atggtgtcgg agattacccc tttactcatc
     8221 atcaccaaac tggagcagta tgactacgcc ggagcgaccg ccatcgccgt ggtcatgctg
     8281 gtgatttcct tcatcctgct gctcatcatc aacggcctgc aatggtggag cacacatcgc
     8341 catggggtag ggaggtaacc atgactattg cagttgcaat tcccgccaac aagtaccaca
     8401 gcaaggtcgc ccaaaaccgg gccatcgccg aaccagcctg gctgcgctgg ttgctgatcg
     8461 gctcggcact ggcctttctt ggcctgttcc tgttcgtgcc gctggcagcg gtattcaccg
     8521 aagccttgcg caagggcttt acggtatacc tcgcggcatt tgacgaaccg gatgcgctat
     8581 cggcaatcaa gctgaccttc attgccgccg ccatcgccgt tccgctgaac ctggtatttg
     8641 gcgtaaccgc tgcctgggcc atcgccaagt tcgagttcaa aggcaagagc gtgctgatta
     8701 cgctgatcga cctgccgttt gccgtgtcgc ccgtcattgc cgggctggtg ttcgtgctga
     8761 tcttcggctt gcaggggtgg ttcggcagtt ggctttccga ccacgatatc aaggtcatct
     8821 ttaccatacc cggcatcgtg ctggccacca tattcgtcac ttttcccttt gtggcgcgcg
     8881 agttgatccc gctgatgcag gcgcagtgca aggacgagga agaagccgcc atcgtactgg
     8941 gcgcctcagg ctggcagatg ttctggcgtg tcacgctgcc caacatcaaa tggggcctgc
     9001 tgtacggggt gattctctgt aacgcccgcg ccatgggcga atttggcgca gtgtcggtgg
     9061 tatccggcca cgtccgcgga ctgaccaaca cgctgccgct gcacgtcgag attctgtaca
     9121 acgaatacaa ctacgtcgct gcttttgcct gcgcctcgat cctggcattc ctggcactgg
     9181 tgacgctggc attgaagaca ctggtggaat ggcaggccag tcgcgccgca accacactcg
     9241 aataaggaga tcacatgggc atcgaaatcc gcaacatcag caagaatttc ggcagtttca
     9301 gcgcactgaa cgacatcaca ctggatgtac cgagcggcga actggtggcg ctgctcggcc
     9361 cttccggctg cggcaagacc accctgctgc gcatcatcgc cgggctggaa tccccggata
     9421 acggcggcgt gctattcaat ggcgaggaca ctacccagcg ccacgtgcgg gagcgccagg
     9481 tcggtttcgt gttccagcat tacgccttgt tccgccacat gacggtgttc gaaaacgtgg
     9541 cttttggcct acgggtaagg ccgaaaaaga cccgcccgga cgatgccgaa atcagccgcc
     9601 gcgtgcacaa acttctcgac ctggtacaac tggactggct ggccgaccgc tacccgcctc
     9661 aactttccgg cggccagcgc cagcgtatcg cgctggcacg tgcgctggca gtcgaaccca
     9721 aagtactgct gctggatgag cccttcggtg cactggacgc acaggtgcgc aaggatttgc
     9781 gccgctggct gcgccgcctg cacgacgagc ttcacatcac cagcgtattc gtcacccacg
     9841 accaggaaga ggcgctggaa gttgccgatc gcgtcgtggt catgaacaag ggcgtggtgg
     9901 agcagatcgg caccccgagc gaagtttacg acaacccggc atcgcccttt gtctaccact
     9961 tcctcggcaa cgtcaacctg tttcaaagcc gcgtgcacgc agggttcgcc cgtgtcggcg
    10021 acatcgagct ggaagccccg gagtaccacg gcgccgagaa tgccccggcg gtggcctatg
    10081 tgcgcccgca cgacatcgac atccggcgcg aagccggtgg agattcggcg ttcaaggtcg
    10141 tggtgaacca catccacccc atcggttcgg tagtacgggt tgaactggag cgcgaggatg
    10201 aaagcgagct aatcgaagcg gaactgaccc gtgaacgctt ccgcgaactc gacctgcgta
    10261 ctggcgactg ggtttttgtc cggccgcgca acctgcgggt tttcctgcaa taacttaaaa
    10321 aatgtccaaa aaaatccccg cacattttgg aatgtacggg gatttcttcg actcattgct
    10381 ttatcctgga cctggcagga ctctgccagc tttaaatcag gatttatcgc gacgggttcc
    10441 agtccgtttc cccgaccgga accttaagcg agtcaaaaat cagttcaccg catccttcag
    10501 cgccttgccg gcggtgaatg caggggcctt gcgggcggcg atcttgattt ccttgccggt
    10561 ttgcgggttg cggccagtgc gcgcggcgcg gctgctcacc ttgaaggtgc caaagccaat
    10621 caaggtgacg ttgtcgccct ttttcagagc cgcggtcacg gaattagtga tggcatctaa
    10681 tgccttgcct gcatcagcct tggtcaggcc ggcttcggcg gcaatggcgt cgatcagttc
    10741 ggatttgttc acgttaggat atccctgttg ttggaaggta tgcgcacttt gctggctaaa
    10801 gcgcatgatg tcaagcttgg gcggcgaaaa cagggttttc ggaagtgctc tagttatttc
    10861 ttgcgcgccc ttggatgcgc ctgatcgtaa accttggcga ggtgctgaaa atccaggtgg
    10921 gtatagacct gcgtggtgct gatgttggcg tgacccaaca tttcctgcac cgcgcgcaga
    10981 tcaccgctgg attgcagcac gtgcgtcgcg aagctgtggc gcagaatatg gggatgcacg
    11041 tctccggcaa cgccctgtct gatcgcccag tacttgaacc ggtactgtac cgcacgcggc
    11101 gtcatgcgct tgccgttacg ggtgacgaac agcgcctgct cgtcgtccag tttcatcatg
    11161 gcacgcaacg gcaaccactc tcggatggcg gcgatggcgt ggctgcctac cggcacgatg
    11221 cgggtcttgg cacccttgcc ggtgacggtg acggtgccgt taccgagatc cagaccgctc
    11281 ggctccagcg acaccagttc tgccagacgc aggccggacg aataaaacag ctccagcatg
    11341 gcgtggtcac gcattgctag ggcatcccca tcggtaattt cgaccaaccg tcccgcctgt
    11401 tccggcgaca gcgcctgcgg cagggatttg gccgacttgg gcggacgcat gccaacgcaa
    11461 ggattgccga caaagccatg gcgcttgccc agataggcgt agaacccgcg ccatgcggac
    11521 agcatgcggg cgatgctttt gccgcccaag ccacgtccgt gcagggtggc gacgtaacgc
    11581 cggatctgtg gcggctgcaa ttgctgcaag gcggtggttc cagccagttc gatcagcgta
    11641 cggatatcgc gcccgtagtt ttgacaggta agcgcgctta ggccgcgctc gtgcgcaagg
    11701 tgattgaggt aggcggccag gtagtccaag ctactggatg tatctgagta gggcagcact
    11761 cagcagttcg ccgatgcgct tgagatatac cgtacccatt tccgggtaga accgctgctc
    11821 gtcctcgctc gccatggcaa gcagcccgaa aacctcctcg ccacgcaggg cgacaaacgc
    11881 gtaggacttc agcggcgcat tggctgcgcc aaacaaatcg cttagatccg ggccggggtg
    11941 atgtccacag taaggactcg ccagaccttg cgcccagatg cgcagctcct cgctcagtgc
    12001 gccgaactcc tcactggcgg cgtcctcctt gttgagcggt ttcgcccaca ggcggatggc
    12061 gacatggggc accttgaagt cctcgcgcaa gtgctccacg accaaccgca tcaggatatc
    12121 gaaactgggg gacccgagca tggccaggca cagacgatgc accttgtcgc cgatggcatc
    12181 gttctcttcg ccgaattgca gcagttccgc cagcttgacc tccagcacgc gaatcttgtc
    12241 acgctgcgcc agttgctggc gctctgccag tgaaacggta ccgctgccat gcgggctggg
    12301 caggtagatc tccgtcagca ggtgggcatt gcgctcgaaa aactgggggt ctgcgcgcag
    12361 gtaatcggtg ctctggtctt cagtaagttg catgagcact cctcggttaa agcgggattt
    12421 ctccctcgaa tacggtaacg gccggcccgg tcatcatgac ggtttgccct tcgcccgccc
    12481 aactgatgga cagctcacca ccgcgcatgg ccacagtgac gggcgaagcc agttttccca
    12541 agcgaatgcc agccacagcg gcggcgcagg caccagtgcc gcaagccaga gtttcgccag
    12601 cgccgcgcac gtacacacgc aggttgatac ggtgcgggtt gactatgtgc atgaacccct
    12661 caatgacgcg ctgcggaaaa cgctggtgtc gctcgatcag cggaccctgt tcggcga
//
LOCUS       DAOMFA010000030        12594 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_477_length_12594_cov_5.168993, whole genome shotgun sequence.
ACCESSION   DAOMFA010000030 DAOMFA010000000
VERSION     DAOMFA010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 12594)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12594)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12594
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_477_length_12594_cov_5.168993"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            159..1598
                     /gene="pyk"
                     /locus_tag="PKW44_02740"
     CDS             159..1598
                     /gene="pyk"
                     /locus_tag="PKW44_02740"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013149067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HQR50539.1"
                     /translation="MTILRKTKIVATLGPASNSEEVLNRMAEAGVDLVRLNFSHGTAR
                     DHVATADLVREIAARHRRPIGVLCDLQGPKIRVGKFAEGKIQLAAGDAFILDAECKLG
                     NQERVGLDYKTLPTEVEAGAILLLDDGRIVMTVRQVLGTEIHCVVDVGGSLSNNKGIN
                     RKGGGLAAEALTEKDKEDIKTAVALDADYIAVSFPRSGADVQHARDLVRAAGGHAGII
                     AKVERAEAIDALEEIIDASDAIMVARGDLGVEVGDAAVPGLQKRMIRLARARNKLVIT
                     ATQMMESMITSPIPTRAEVSDVANAVLDGTDAVMLSAESAAGKYPVEAILAMDRVCRE
                     AEKDFDSQNFKAKASKEFLRIDESIAMAAVHVAQHLKVKAIAALTESGTAVMWLSRAN
                     TLVPIYALASKKETRRKLTLFRGVYPYPFEQSTNDRDQILHDAEDELRMRGAVKDGDL
                     ILLTIGEPVGVSGGTNTLKIVKVGEHRKS"
     gene            1716..2786
                     /gene="fba"
                     /locus_tag="PKW44_02745"
     CDS             1716..2786
                     /gene="fba"
                     /locus_tag="PKW44_02745"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013646216.1"
                     /note="catalyzes the reversible aldol condensation of
                     dihydroxyacetonephosphate and glyceraldehyde 3-phosphate
                     in the Calvin cycle, glycolysis, and/or gluconeogenesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase class II"
                     /protein_id="HQR50540.1"
                     /translation="MALVSLRQLLDHGAEHGYGLPAFNVNNMEQIHAIMQAADEVDSP
                     VILQGSAGARKYAGEPFLRHLVTAAVEAYPHIPVVMHQDHGASPAVCIMAIRSGFSSV
                     MMDGSLKDDMKTPASYDYNVEVTRKVVEMAHAIGVSVEGELGCLGSLETGMAGEEDGH
                     GAEGVLDHSSLLTDPEEAASFVKATGVDALAIAIGTSHGAYKFTRPPTGDVLAIQRIK
                     EIHARIPNTHLVMHGSSSVPQEWLKIINEFGGDIGETYGVPVEEIVEGIKHGVRKVNI
                     DTDLRIASMGAVRRFLGQEQNRKELDPRKFLLAATKAMSDICKARFEAFGSAGQAGKI
                     KAISCDRMAEKYKKGELAAIVK"
     gene            2844..3599
                     /locus_tag="PKW44_02750"
     CDS             2844..3599
                     /locus_tag="PKW44_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018507540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HQR50541.1"
                     /translation="MTLQALLFDVDGTLADTERDGHRPAFNLAFRELGLDWDWDVELY
                     GKLLAVTGGKERIRYYVDSFLPDYRKPADFDDLVAELHRTKTRHYTSLLAEGRIPMRP
                     GVKRLIAEARLAGLRLAIATTTTPENVSALLRYSLEEDAMGWFEVIAAGDIVPAKKPA
                     PDIYEWALCELRLGPESCLAFEDSRNGLKASLGAGLKTLVTVNGYTAGEDFHDALAVL
                     SDLGEPQAPFEVLAGEAYGNGWVDVELLRCWCR"
     gene            complement(3625..4533)
                     /locus_tag="PKW44_02755"
     CDS             complement(3625..4533)
                     /locus_tag="PKW44_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HQR50542.1"
                     /translation="MIHFTLRQLNVFDAVARHLSYSRAAESLYLSQPAVSMQIKQLEE
                     SVGVPLLEQLGKKIYLTAAGREFHNHARAILQQLQDAEAMFAEQKGMSGKLVICVAST
                     ASYFTPQLLAEFCKRHPKAEVSLNVTNREQLLQHLAHNEMDMAIMGRPPEGLDLDATP
                     FMENRLAIIAPPNHPLAGERNIPMSRLAEETFLVREQGSGTRIAMERFFAEKGIRLTT
                     GTEMSTNEAIKQAVQAGMGLGILSLHTVSLELETGRLIVLDVQNFPILRNWYVVHRKG
                     KRMTAVAQAFKEFLLEEADALVGSAA"
     gene            4672..>5071
                     /locus_tag="PKW44_02760"
     CDS             4672..>5071
                     /locus_tag="PKW44_02760"
                     /EC_number="4.1.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011313150.1"
                     /note="catalyzes the carboxylation of D-ribulose
                     1,5-bisphosphate in carbon dioxide fixation; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose 1,5-bisphosphate carboxylase"
                     /protein_id="HQR50543.1"
                     /translation="MDQSARYADLSLKEEDLIKGGKHILVAYKMKPKAGYGYLEAAAH
                     FAAESSTGTNVETSTTDDFTKGVDALVYYIDEATEDMRIAYPMDLFDRNMTDGRMMMV
                     SILTLVIGNNQGMGDIAHGKIYDIYFPERAI"
     assembly_gap    5072..5092
                     /estimated_length=21
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5093..6063
                     /locus_tag="PKW44_02765"
     CDS             <5093..6063
                     /locus_tag="PKW44_02765"
                     /EC_number="4.1.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011313150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribulose-bisphosphate carboxylase"
                     /protein_id="HQR50544.1"
                     /translation="GPSKDISDMWRVLGRPIKDGGYIAGTIIKPKLGLRPEPFAKAAY
                     QFWLGGDFIKNDEPQGNQVFCPAKKVYPMVYDSMKRAMDETGQAKIFSANITADDHYE
                     MLHRADFILETFGPDADKVAFLVDGFVGGPGMITTARRQYPAQYLHYHRAGHGMITSP
                     SAKRGYTAFVLSKIARLQGASGIHVGTMGYGKMEGEHDDRLIAYMIERDECQGPVYYQ
                     KWYGMKPTTPIISGGMNALRLPGFFENLGHGNVINTSGGGSYGHIDSPAAGAVSLRQS
                     YECWKSGSDPIEYAKEHKEFARAFESFPGDADKLYPGWREKLGVHK"
     gene            6175..6987
                     /locus_tag="PKW44_02770"
     CDS             6175..6987
                     /locus_tag="PKW44_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbbQ/NirQ/NorQ/GpvN family protein"
                     /protein_id="HQR50545.1"
                     /translation="MSNHFDVNQYLLKEEPFYQPTGNEVALYEAAYAARLPVMLKGPT
                     GCGKSRFVEHMAWKLGKPLITVACNEDMTASDLVGRYLLDANGTRWLDGPLTLAARYG
                     AICYLDEIVEARQDTTVVIHPLTDHRRTLPLDKKGELVAAHPDFQLVISYNPGYQSLM
                     KDLKQSTKQRFTAFDFDYPSAALETDILAKETGLDAETAGKLVKIGHTARNLKGHGLD
                     EGISTRLLVYAATLIKGGVVPRDACRMALVRPITDDADIRDTLDHAVDATFA"
     gene            7003..9267
                     /locus_tag="PKW44_02775"
     CDS             7003..9267
                     /locus_tag="PKW44_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HQR50546.1"
                     /translation="MAVAAPTYQGKLPDYWKQLDNRFPQVMAVFDECMEDALKVLSND
                     GIAAYLESASFLGRMGRGPEPILIFLEEWPPIAKVLGEASLPAIMDCIRQMFKSPNGK
                     AITPFLQALPAVARRLKTQEQMRDYLDLVVDFMNRTTGSIHGHQATFPSPGLPDFLGK
                     APYLVSQVSIAGLKKWVDYGIRNYKDNPDRQSDFFSLQSADSKAMLQRERHGTLLADH
                     ERKLDLYLRGLWQDSDYLVPYSTGFDELRKPVPYYDKLGIRLPDVYGDARGVGGIDRY
                     RAVLAHIAAHRRWTTPIFADNYSPFQRMAVEIFEDSRVEYLAMREYPGLRRLFLALHP
                     KPAEDACDPEQVSAIRHRLAMLSRAILDADHGYKNPHIIEFAQRFHAIMAQGESCTQE
                     IADLAALFVAKTRRQSDQLPKVHFLDTEVDYRDDNRHMWRFHEESDDEEQFEEHRQAD
                     DEGEIKGLPPRHYHEWDYNTQSYRPDWVSVYEALHPQGNAAEIDRLLEKHAALAKRLK
                     RLLDLLKPQQKVRIRFQEEGSELDLDVALRSLIDYKSGSAPDPRINMSHRNDGRDIAV
                     MLLLDLSESLNEKVAGTEQTLLQLSQEAVSLLAWAVEQLGDSFAIAGFHSNTRHEVRY
                     LHIKGYGEHWDEAVKGRLAAMQAGYSTRMGAAMRHAGHFLEHRQADKKLMLILTDGEP
                     ADIDAKDAKLLIQDAHKAVQELDRQGIYTYCINLDPKADEYVSDIFGKNYTVLDNVAR
                     LPERLPQLFMALTK"
     gene            complement(9388..9726)
                     /locus_tag="PKW44_02780"
     CDS             complement(9388..9726)
                     /locus_tag="PKW44_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020163005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HQR50547.1"
                     /translation="MSTIPACPQCTLENTYPDGANYVCADCGYEWPMAEAAEADDDGL
                     VVKDANGTVLQDGDAVVLIKDLKVKGSSITLKQGTKVKSIRLVGGDHGVDCKMDAGNF
                     MLKAEFLKKV"
     gene            9956..10855
                     /locus_tag="PKW44_02785"
     CDS             9956..10855
                     /locus_tag="PKW44_02785"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020182894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HQR50548.1"
                     /translation="MTQPLLQSSLNSLPLINQGKVRDIYDVDADKMLIITTDRLSAFD
                     VVLPTPIAGKGAVLTQIANFWFDKLSAIIPNHLTGIAPESVVKDVAEHAQLGERAIVV
                     KKLKPLPIEAVVRGYLVGSGWKEYQKSGTVCDIALPAGLQEASRLPQPIFTPSTKAEL
                     GTHDENITFEQTVALVGAELASKVRDTAIALYQAAAEYALTKGIIIADTKFEFGLDAA
                     GTLYLIDEALTPDSSRFWPADQYQVGSNPPSFDKQYVRDWLEASGWNKQAPGPELPAE
                     VAARTSDKYREALRQLTGRDIKL"
     gene            10855..11136
                     /locus_tag="PKW44_02790"
     CDS             10855..11136
                     /locus_tag="PKW44_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019897320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HQR50549.1"
                     /translation="MGLLRDTAVRLKTELAVYRRVLRHPHTPLLAKVLLGLAIGYALL
                     PFDLIPDFIPVLGHLDGLVIVPGLVILALRLIPQQVVAECRQEVERTNG"
     gene            11129..11749
                     /locus_tag="PKW44_02795"
     CDS             11129..11749
                     /locus_tag="PKW44_02795"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HQR50550.1"
                     /translation="MADIKKFIAGYKRFHDNYFVGNQELFGRLKEGQNPSAMVIACSD
                     SRVDPAILLDCQPGDLFMVRNVANLVPPYERGAGLHGVSTALEFGVDVLGVAHLIVLG
                     HSSCGGVRALMDGTQGDFMGNWVNIAARAKQRVLAEFPDASAQEQCHACEEASILVSL
                     ENLMTFPWIRERVESGQLTLHGWYFDIDSGDLVAYDPSLGRFEPML"
     gene            11787..12335
                     /gene="ppa"
                     /locus_tag="PKW44_02800"
     CDS             11787..12335
                     /gene="ppa"
                     /locus_tag="PKW44_02800"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013149064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HQR50551.1"
                     /translation="MALNNVPSGKNLPDDFNVVIEIPMQGDPVKYEVDKESGAIFVDR
                     FMGTAMQYPCNYGYIPHTLSEDGDPVDVLVQTPVPLIPGVVVRCRPLGVLKMTDEAGP
                     DAKVLAVPVDKVCGLYSHFKDYNDVSGWRLTMIAHFFEHYKDLEKGKWVKIDGWGSLD
                     EARQEILAGVERYNNADEKPAF"
     gene            12358..>12594
                     /locus_tag="PKW44_02805"
     CDS             12358..>12594
                     /locus_tag="PKW44_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029493.1"
                     /note="catalyzes the formation of D-fructose
                     1,6-bisphosphate from D-fructose 6-phosphate in
                     glycolysis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphate--fructose-6-phosphate
                     1-phosphotransferase"
                     /protein_id="HQR50552.1"
                     /translation="MAKVQLKNAFYAQSGGVTAVINASACGVIQTARKHPDRIGKVYA
                     GRNGIVGALTEDLIDTSLESDAAIAALRSTPSGAF"
BASE COUNT         2561 a         3785 c         3876 g         2351 t
ORIGIN      
        1 atttccaccg gcggcggatc cttcctcgag ttcgtcgagg gcaagaaact gccggccgtg
       61 gaaatgctcg aacagcgcgc gcaaggctga atgcagtaag caatcctgct gcgggaagcc
      121 tggcgatcag gcttcccgcc accattttga aagaccgaat gaccatacta cgtaaaacaa
      181 aaatcgtcgc tacgctggga cccgcctcca atagcgagga ggtgctgaat cgcatggcgg
      241 aagccggcgt cgatctggtg cgcctcaact tttcgcatgg cacggcacgc gaccatgtcg
      301 cgactgcaga tctggtgcgc gagatcgccg cccgccaccg ccgacccatc ggcgtcctgt
      361 gcgacctgca agggccgaaa atccgtgtcg gcaaattcgc cgagggcaag atccaactcg
      421 ccgcgggcga tgcgttcatc ctggatgcag agtgcaagct gggcaatcag gagcgcgtcg
      481 gcctcgatta caagaccctg ccgaccgagg tggaagcggg cgccatactg ttgctggatg
      541 acgggcgcat cgtgatgacg gtgcggcaag tgcttggcac ggagatccat tgcgtggtcg
      601 atgtcggcgg ttccttgtcc aacaacaagg gcatcaaccg caaaggtggt ggtctggccg
      661 ccgaagcgct gaccgaaaaa gacaaggaag acatcaagac tgccgtggcg ctcgatgcgg
      721 actatatcgc cgtatccttc ccgcgcagcg gtgcggatgt gcagcatgcg cgcgacctgg
      781 tgcgcgccgc cggcggccat gccggcatca tcgccaaggt ggagcgtgcc gaggcgatcg
      841 atgcgctgga agagatcatc gacgcatcgg atgccatcat ggtggcgcgc ggcgatttgg
      901 gggtggaagt cggcgatgcc gccgtgcccg gtctgcagaa gcgcatgatc cgcctggcac
      961 gcgcccgcaa caagctggtg atcaccgcca cgcagatgat ggaatccatg attaccagcc
     1021 cgatcccgac ccgtgccgaa gtctccgacg tggccaacgc ggtgctggac ggcaccgacg
     1081 ccgtgatgct gtcggcggaa agcgctgccg gcaaatatcc ggtcgaggcc atcctggcca
     1141 tggaccgcgt gtgccgcgag gccgaaaagg actttgacag ccagaacttc aaggcgaaag
     1201 ccagcaagga gttcctgcgc atcgacgaat ccatcgccat ggccgccgtg cacgtggcgc
     1261 agcacctcaa ggtcaaggcc atcgcagcgc tcaccgagtc cggcacggcc gtgatgtggc
     1321 tgtcgcgcgc caatacgctg gtgcccattt atgcactggc ttcgaagaag gagacacgcc
     1381 gcaagctgac tttattccgc ggcgtctatc cctatccgtt cgagcagagt accaacgacc
     1441 gcgaccagat cctgcatgat gcagaagatg aactgcgcat gcgcggcgcg gtcaaggacg
     1501 gcgacctgat cctgctgacc atcggcgaac ccgtcggtgt ctctggcggc actaataccc
     1561 tcaagatcgt caaggttggc gagcacagga aaagctgatt taatcgaact gacagggcgc
     1621 ccgcgccctc taagttgaat tcacgggaga cacttcccga caagtctgtt tcgctgcaat
     1681 caatcgatct gcggcgggac aaggaggaga acatcatggc actcgtatca ctacggcaat
     1741 tactcgatca cggcgccgaa cacggctacg gcctgccggc attcaacgtc aacaacatgg
     1801 aacagatcca cgccatcatg caggcggcgg acgaggtgga cagtccggtg atcctgcaag
     1861 gctccgccgg cgcccgcaag tatgccggcg agcctttcct gcgccacctg gtcaccgcag
     1921 ccgtggaagc ctatccgcac attcctgtgg tcatgcacca ggatcacggt gcctcgccgg
     1981 cagtgtgcat catggccatc cgctctggtt tttccagcgt gatgatggac ggctcgctca
     2041 aggatgacat gaagaccccg gccagctacg actacaacgt cgaggtcacg cgcaaggtgg
     2101 tcgaaatggc gcacgccatc ggcgtctcgg tggaaggcga actgggctgt ctgggttcgc
     2161 tggagaccgg catggccggc gaagaggacg gccatggcgc tgaaggcgtg ctggaccatt
     2221 ccagcctgct caccgatccg gaggaagcgg catctttcgt caaggctacc ggggtggacg
     2281 cactggccat cgccatcggg acctcgcacg gtgcctacaa gttcacccgc ccgccgaccg
     2341 gcgacgtgct ggccatccag cgcatcaagg aaatccacgc ccgcattccg aacacccatc
     2401 tggtgatgca cggctcgtct tcggtgccgc aggaatggct gaagatcatc aacgagtttg
     2461 gtggcgacat cggcgaaact tacggcgtgc cggtggagga gattgtcgaa ggcatcaagc
     2521 atggcgtgcg caaggtcaac atcgataccg acctgcgcat cgcttcgatg ggcgcggtgc
     2581 gccgcttcct ggggcaggag cagaaccgca aggaactcga cccgcgcaag ttcctgctag
     2641 ccgccaccaa ggccatgagt gacatctgca aggcgcgctt cgaagccttt ggttctgccg
     2701 gccaagctgg taagatcaag gccatttcct gtgacaggat ggccgagaag tacaagaagg
     2761 gcgagctggc ggccatcgtc aagtaagccg tatggttcat aaaggcggcc ccggccgcct
     2821 ttttttgcga gcgagggaat gaaatgacgc tgcaagcttt gttattcgac gtcgacggca
     2881 ctctggccga taccgagcgc gatggccacc ggccggcatt taacctggcc ttccgggagc
     2941 ttggcctgga ctgggactgg gatgtcgagc tctacggcaa actgctggcc gtgaccggcg
     3001 gcaaggagcg catccgctac tatgtcgaca gcttcctccc cgactatcgc aagccggcgg
     3061 atttcgacga cctcgtcgcc gaactgcacc ggacgaagac gcgccattac accagcttgc
     3121 tggccgaggg gcgcatcccc atgcggcccg gcgtcaaacg gctgattgcc gaggcgcgac
     3181 tggccgggct acgcttggcg atcgcgacca ccaccacgcc ggaaaacgtc agcgcgctgc
     3241 tgcgctacag cctggaagaa gacgcaatgg gctggttcga ggtcatcgcc gccggcgata
     3301 tcgtgccggc caagaagccg gcgccggaca tctatgaatg ggcactgtgc gagctgcggc
     3361 tgggtccaga atcctgcctg gcgttcgagg attcacgcaa cggcctcaag gccagtctgg
     3421 gcgccggact gaagacgctg gtcacggtca acggctatac tgcaggcgag gatttccacg
     3481 acgcgctggc cgtgctgagc gacttgggcg agccgcaggc gccattcgag gtgctggcgg
     3541 gggaggccta cggtaatggc tgggtggatg tggagttgct gcggtgctgg tgtcgttgag
     3601 tcaaagtggg gtatgggctg ccgactaggc agcgcttccc accagagcgt ctgcctcttc
     3661 cagcaggaac tccttgaatg cctgtgccac ggccgtcatg cgcttgccct tgcggtgcac
     3721 cacgtaccag ttgcgcagga tgggaaagtt ctgcacgtcc aatacgatga gacggccggt
     3781 ttccagttcc agcgaaacgg tgtgcagcga caggatgccc agccccatgc cggcctgcac
     3841 cgcctgcttg atggcttcgt tggtgctcat ctcggtgccg gtggtaaggc ggatgccctt
     3901 ttcggcgaag aagcgctcca tggcgatgcg ggtgccagaa ccctgctcgc gcaccaggaa
     3961 ggtttcctcc gcaaggcgtg acatcgggat gttgcgttct cctgccaaag gatgattggg
     4021 tggcgcgatg atggcgaggc ggttttccat gaacggcgtg gcatcgaggt ccagcccttc
     4081 cggcgggcgc cccatgatgg ccatgtccat ttcgttgtgc gccaaatgct gtagtagctg
     4141 ctcgcggttg gtgacgttga ggctaacttc cgccttgggg tggcgcttgc agaattcggc
     4201 cagcagttgc ggagtgaagt agctggcggt gctggcgacg cagatgacga gtttgccgct
     4261 catgcctttc tgttcggcga acatggcttc ggcgtcctgc agttgttgca gtatggcgcg
     4321 ggcatggttg tggaattccc gcccggccgc ggtgaggtag atttttttgc ccagctgttc
     4381 cagcagcggc acgcccacgc tttcctccag ctgcttgatc tgcatggaga cagccggctg
     4441 cgacaggtac aggctttcgg ccgcgcgcga ataggagaga tggcgggcga cggcatcgaa
     4501 gacattcagc tggcgaaggg taaagtgaat cataagtaaa atattatcat atcaatcaaa
     4561 aactctgact gttacttatg gaaaagtgct tgtatagtgt taacaacctt gaacgcaatc
     4621 caagcgttcc tgtgtaaaaa tttttagaca tttaatcaag aggagatagc gatggatcag
     4681 tcagcacgtt atgcagatct cagcctcaag gaagaagatc tgatcaaggg tggcaaacac
     4741 attctggtgg cttacaagat gaaacccaag gccggttacg gttatctgga agccgccgcc
     4801 cacttcgccg cggaatcctc taccggtacc aatgttgaaa cgagtacaac cgacgacttc
     4861 accaagggtg ttgatgcgct ggtgtattac atcgacgaag ccacggaaga catgcgcatt
     4921 gcctatccga tggatttgtt tgatcgcaat atgacggacg gtcgcatgat gatggtgtcc
     4981 atcctgacac tggtcattgg taataatcag ggcatgggtg atattgctca cggcaaaatt
     5041 tacgacattt atttcccgga gcgcgctatt cnnnnnnnnn nnnnnnnnnn nnacggcccg
     5101 tccaaggata tctccgatat gtggcgcgtc ttgggccgcc caatcaagga tggcggctac
     5161 atcgccggaa ccatcatcaa gcccaagctg ggcctccgcc ccgagccgtt tgccaaggcc
     5221 gcttaccagt tctggctggg tggcgatttc atcaagaacg acgagccgca aggcaatcag
     5281 gtgttctgcc cggccaagaa ggtttatccc atggtctatg actcgatgaa gcgtgccatg
     5341 gatgaaaccg gtcaagccaa gattttctcc gccaatatta ctgctgacga ccattatgaa
     5401 atgctgcatc gcgccgattt cattctggaa actttcggcc cggatgccga caaggtcgct
     5461 ttcctggtcg acggttttgt cggtggcccc ggtatgatca ccaccgcacg tcgccagtat
     5521 cccgcccaat acctgcacta tcaccgcgcc ggccatggca tgattacctc gccctcggcc
     5581 aagcgcggct acacggcgtt tgtgttgtcc aagatcgcgc gtctgcaagg cgcttccggc
     5641 attcacgtcg gcactatggg ctacggcaag atggaaggcg agcacgacga ccgcctcatc
     5701 gcctacatga tcgagcgcga tgagtgccaa ggcccggttt attaccagaa atggtacggc
     5761 atgaagccca ccacgcctat catctccggc ggcatgaacg cgctgcgcct gccaggcttc
     5821 ttcgaaaacc tgggccacgg caacgtgatc aacacctccg gcggcggctc ctatggccac
     5881 atcgacagtc cggcggccgg tgcggtctcg ctgcgccagt cctacgagtg ctggaaatca
     5941 ggttccgatc cgatcgaata cgccaaggag cacaaggaat ttgctcgtgc cttcgagtcc
     6001 ttccccggag atgcggacaa gctgtatccg ggctggcgcg aaaagctggg cgtgcacaag
     6061 taaactgagg gttgtggcac cgctgtaacc gcaaagcccc tgcactcaat ctcctgcagg
     6121 ggcttttttt cgggcttttt catagtaatt tggtcggaat taaaggtgga aaacatgagc
     6181 aaccattttg acgtgaacca gtatctcctc aaggaggagc ctttctacca gccgaccggc
     6241 aacgaggtcg cgctgtacga agcagcctat gcggcgcggc taccggtgat gctgaaaggt
     6301 ccgaccggct gcggcaaatc gcgcttcgtc gagcacatgg catggaagct gggcaagccg
     6361 ctgattaccg tggcctgcaa cgaggacatg accgcctccg acctggtggg gcgctacctg
     6421 ctggacgcta acggcacgcg ctggctggat ggcccgctga cgctggcggc gcgctacggg
     6481 gcgatctgct atctcgatga aatcgtcgag gcgcgccagg acaccaccgt cgtcatccat
     6541 ccgctcaccg accaccgccg tactttgccc ttggacaaga agggcgagtt ggtggctgca
     6601 cacccggatt tccagctagt gatttcctac aatccgggct accagagcct gatgaaggac
     6661 ctcaagcaat cgaccaagca gcgcttcacc gccttcgatt tcgactaccc gtcggccgcg
     6721 ctggaaaccg acattctggc caaggaaacc gggctggacg cggaaaccgc cggcaagctg
     6781 gtgaagatcg gccacacggc gcgcaacctc aaggggcacg gtctggacga aggcatttcc
     6841 acccgcctgc tggtctacgc cgccaccctg atcaaaggcg gtgtcgtgcc gcgcgacgcc
     6901 tgccgcatgg cgctggtgcg gccgatcacc gacgacgcag atatccgcga cacgctggat
     6961 catgcggtgg atgcgacgtt cgcctgaagc gggcgctgaa tgatggccgt cgcagcaccg
     7021 acctatcagg gcaaattgcc tgactactgg aagcagttgg acaaccgctt cccgcaggtc
     7081 atggccgtgt tcgacgagtg tatggaagat gcgctcaagg tgctgagcaa cgatggcatc
     7141 gccgcctacc ttgagagcgc aagcttcctg ggcaggatgg ggcgcggccc cgagcctatt
     7201 ctcatctttc tggaagagtg gccgcccatc gccaaggtgc tgggcgaagc ttcgctgccg
     7261 gcgatcatgg actgcatcag gcaaatgttc aaatcgccca acggcaaggc catcacgccc
     7321 ttcctgcagg ccttgcccgc cgtggcgcgg cgcctgaaaa cgcaggaaca aatgcgcgac
     7381 tacctcgatc tggtcgtcga tttcatgaac cgcaccaccg gctccatcca cggccaccaa
     7441 gccactttcc ccagccccgg actgcctgac tttctcggca aggcgcccta tctggtcagc
     7501 caggtctcca tcgccgggtt gaagaagtgg gtggactatg gcatccgcaa ctacaaggac
     7561 aaccctgacc gtcagagcga cttcttcagc ctgcaatcgg cggacagcaa ggccatgctg
     7621 caacgtgagc gccacgggac gctattggcc gaccacgagc gcaagctgga cctctacctg
     7681 cgtggcctgt ggcaagacag cgactacctg gtgccgtatt ccaccggctt cgacgagttg
     7741 cgcaagccgg tgccttatta cgacaagctc ggcatccgtc tgccggatgt gtacggggac
     7801 gcgcgcggcg tcggcggcat cgaccgctat cgtgccgtgc tggcgcatat cgccgcccac
     7861 cggcgctgga ccacgccgat cttcgccgac aactacagcc cgttccagcg catggcggta
     7921 gagatcttcg aggacagccg ggtggaatat ctcgccatgc gcgaatatcc tggcttgcgc
     7981 cgcctattcc tggcgttgca ccccaagccg gcggaagatg cctgtgaccc ggagcaggtc
     8041 tcggcgatca ggcacaggct ggcgatgctg tcgcgcgcca tcctggatgc cgaccacggc
     8101 tacaagaacc cacacatcat cgagttcgcc cagcgttttc acgccatcat ggcgcagggc
     8161 gaatcctgca cgcaggaaat cgccgatctg gctgcgctct tcgtggccaa gacccgccgc
     8221 cagagcgacc agctgcccaa agtgcatttc ctcgataccg aggtggacta tcgcgatgac
     8281 aatcgtcata tgtggcggtt ccacgaggaa agtgacgacg aggagcagtt cgaggagcac
     8341 cgccaggcgg acgatgaagg cgagatcaag ggccttccgc cacgccatta ccacgagtgg
     8401 gactacaaca cgcaaagcta tcgcccggac tgggtgagcg tgtacgaggc gctgcacccg
     8461 cagggcaacg ccgcggaaat cgaccgcctg ctggaaaagc acgcggcgct ggccaagcgc
     8521 ctgaaacgcc tgctcgacct gctcaagccg cagcagaagg tgcgcatcag gtttcaggag
     8581 gagggcagcg aactcgattt ggacgtcgcc ctgcgctcgc tgatcgatta caagagcggc
     8641 tccgcgcccg acccgcgcat caacatgagc cacaggaacg acgggcggga catcgccgtc
     8701 atgctgctgc tggacttgtc ggaatcactg aacgagaagg tggccggcac cgagcagacc
     8761 ctgctgcaac tgagccagga ggccgtctcg ctgctggcct gggcggtgga gcaactgggc
     8821 gacagctttg ccatcgccgg cttccattcc aacacccgcc acgaggtgcg ctacctgcac
     8881 atcaagggtt atggcgaaca ctgggacgag gcggtgaaag gccggttggc ggccatgcag
     8941 gccggctact ccacccgcat gggtgccgcc atgcgccacg ccgggcactt tctggagcac
     9001 cgccaggctg acaagaaact gatgttgatt ttgacggatg gcgaaccggc ggacatcgac
     9061 gccaaggacg ccaagctgct gatccaggac gcgcacaagg cggtgcagga actcgaccgg
     9121 cagggcatct atacctactg catcaacctc gaccccaagg ccgacgagta cgtgagcgat
     9181 atttttggca aaaactacac cgtgctcgac aacgtcgcgc gcctgccgga acggctgccg
     9241 cagctattca tggcgctgac gaagtagtgg ggggggcgtt ccctccccca ggcagggtga
     9301 ggtcagggtg ggggtcgaaa gtttccaccc catcccaccc ttccccctac gaaggggaag
     9361 gagtcgaagc cagtcgccag cgccacctca aaccttcttc aaaaactccg ccttcagcat
     9421 gaagttgccg gcatccatct tgcaatccac gccgtggtcg ccgccgacca gacggatgct
     9481 cttgaccttg gtgccctgct tcagcgtgat ggaggaaccc ttgaccttca aatccttgat
     9541 cagcactacc gcgtcgccat cctgcaacac cgtgccgttg gcatccttta ccaccagccc
     9601 gtcgtcgtca gcctcggcgg cttccgccat cggccattcg tagccgcaat cggcgcacac
     9661 gtagttggcg ccatccggat aggtgttttc cagggtgcat tgcgggcaag ccgggatggt
     9721 ggacatggtg acctcgaagg tgggttggag ggcgcggatt ataaccgacc gatggcgcgg
     9781 gtgtgtaggc ctgagttgca ccaatcctgt tggcgactat agttcgagcc gaaagtggct
     9841 cgccgattca aaccagccgt catgcgtttg tcatgttcaa tccctatcgt cgcttttcac
     9901 ggcccgccag acaggggata attcaacctt tcacaagcaa tccccaagac acccgatgac
     9961 acagccttta ctgcaatcca gcctcaacag cctgccgctg atcaaccagg gcaaggtgcg
    10021 cgacatttac gacgtggacg cggacaaaat gctgattatc accaccgacc ggctgtccgc
    10081 ttttgatgtc gtcctgccga cgcccatcgc cggcaagggc gcggtgctga cgcagattgc
    10141 caatttctgg ttcgacaagt tgagcgccat catccccaac cacctcaccg gcatcgcgcc
    10201 ggaatcagtg gtgaaggacg tggctgagca tgcgcaactg ggcgagcgtg ctatcgtggt
    10261 gaagaagctg aagccgctgc cgatcgaggc ggtggtgcgc ggctatctgg tgggttcggg
    10321 ctggaaggaa taccagaagt ccggcacggt gtgcgacatt gccctgccgg cgggcctgca
    10381 ggaagcctcc aggctgccgc agccgatctt caccccgtct accaaggcgg agctgggcac
    10441 gcacgacgag aacattacgt ttgagcagac ggtggcgctg gtgggcgcgg agctggcgtc
    10501 caaggtgcgt gataccgcca tcgccctgta tcaggcggcg gcggagtatg cgctcaccaa
    10561 gggcatcatc atcgccgaca ccaaattcga gttcgggctg gatgcggcgg gtacgctgta
    10621 cctgatcgac gaggcgctga cgccggattc ctcccgcttc tggccggccg accagtacca
    10681 ggtcggcagc aacccgccca gcttcgacaa gcaatacgtg cgcgactggc tggaggcttc
    10741 cggctggaac aagcaggcgc cggggccgga actgccggcc gaggtggcgg cgaggaccag
    10801 cgacaagtac cgcgaggcgt tgcgccagtt gacggggcgc gacatcaagc tttaatgggg
    10861 ttgctgcgcg acacggcggt ccggctcaaa actgagctgg ctgtttaccg gcgggtactc
    10921 aggcatccac acacgccgtt gctggcgaag gtgttgctgg ggctggcgat cgggtatgct
    10981 ctgctgccgt tcgacctgat cccggatttc atccccgtgc tcggccatct ggatggcctg
    11041 gtgatcgtgc ccgggctggt gatcctggca ttgcggctaa tcccgcagca agtggtggcc
    11101 gagtgccgcc aagaggtaga aaggacaaat ggctgacatc aagaaattta tcgcggggta
    11161 caagcgcttc cacgacaact attttgttgg gaatcaggag ctgttcggtc ggttgaaaga
    11221 ggggcagaac cccagcgcga tggtgatcgc atgttccgat tcgcgggtcg acccggcgat
    11281 tttgctggat tgccagcccg gcgacttgtt catggtgcgc aacgtcgcca acctggtgcc
    11341 gccttatgaa aggggcgctg gcctgcatgg cgtcagtacc gcgctggagt tcggcgtgga
    11401 cgtgctgggc gtggcccatc tcatcgtcct cgggcatagc agttgcgggg gcgttcgcgc
    11461 attgatggac gggacgcagg gggacttcat gggcaactgg gtcaacatcg ccgcccgcgc
    11521 aaaacagcgg gtgctcgccg agtttcctga tgccagcgcc caggagcagt gccatgcctg
    11581 cgaagaagct tccatactgg tatcccttga aaatctgatg actttcccgt ggattcggga
    11641 acgcgtcgag tcaggtcagt tgaccctgca tggatggtat ttcgatatcg atagcggtga
    11701 cctggtggct tacgacccgt ccctcggccg attcgagccg atgctttgaa ttttacttaa
    11761 tggcaattta ctgaaggatc attatcatgg ctctcaacaa tgtaccatcc ggcaagaatc
    11821 ttcccgacga cttcaacgtc gttatcgaaa tccccatgca gggcgacccg gtcaagtatg
    11881 aagtggacaa ggaaagcggc gccattttcg tcgatcgctt catgggcacg gcgatgcagt
    11941 acccctgcaa ctacggttac atcccgcaca cgctgtcgga agacggcgac ccagtggacg
    12001 tgctggtgca gacgccggtg ccgctgatcc ccggcgtggt ggtgcgctgc cgcccgctgg
    12061 gcgtgctgaa gatgaccgac gaggccggcc ccgacgccaa ggtgctggcg gtgccggtag
    12121 acaaggtatg cggcctgtat agccacttca aggattacaa cgatgtttcc ggctggcgcc
    12181 tgacgatgat tgcccacttc ttcgagcact acaaggacct ggagaagggc aagtgggtga
    12241 agatcgacgg ctggggcagt ctggacgagg cgcgccagga aatcctcgcc ggcgtggaac
    12301 gctacaataa cgccgacgag aagccggcgt tctgattgtt caaagcggag gtgcatcatg
    12361 gctaaggttc aactgaagaa tgcgttctat gcccaatccg gcggcgtcac cgctgtcatc
    12421 aatgcttctg cctgtggtgt catccagacc gcgcgcaagc atccggacag gattggcaag
    12481 gtctatgccg ggcgcaacgg catcgtcggc gcgctgaccg aggacctgat cgacaccagc
    12541 ctggagtccg acgctgccat cgccgcgctg cgctccactc cttccggcgc cttc
//
LOCUS       DAOMFA010000031        12464 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_487_length_12464_cov_5.212991, whole genome shotgun sequence.
ACCESSION   DAOMFA010000031 DAOMFA010000000
VERSION     DAOMFA010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 12464)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12464)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12464
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_487_length_12464_cov_5.212991"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1243
                     /locus_tag="PKW44_02810"
     CDS             <1..1243
                     /locus_tag="PKW44_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HQR50553.1"
                     /translation="GKSPEWIVKSAVSYGESIGLPFVPESFWAKSDLYELPPDAKRKK
                     NTHAFIARYQRAKSDINLAFVNTSKEPKRAQEAGISKDGEIVVEYEKRSEHLAPPYAE
                     RDLTNLLVRLARSQTRAIMFLDGHGERSLIGEKNHDLGEFGHQLGKKGFKLANPDLTV
                     AQGVPRSGAMLVIAGPQVDVSPAEVDKIMGYVHSGGNLLWLIDQESLHGLQPLAEYLG
                     LVLTPGIAVDPTSSSNGLDPRTVVGRQYTEHPITKDFSLLTAFPESRQIEVKDASGDW
                     EVTRLISVAQNGWLETGKPEAKIEFDAKRDIPGPVNIAVAMQRKAENVTQRVVVIGNG
                     SFLANAFVASGGNLDLGINIVNWLAGDDKLITIQPKPLKDVNVSLSPTQGTLIFGGFV
                     LVLPLAFLVIGIAIWRRRRTR"
     gene            1240..2121
                     /locus_tag="PKW44_02815"
     CDS             1240..2121
                     /locus_tag="PKW44_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HQR50554.1"
                     /translation="MKSRWLLNIALLLLVAGIALALYLVPQQKDATPEKVKVSSIDPG
                     SVSKISIEFPAKAPVVLEKQDGYWHLVQPYKARASQELARLITSILSASSSNKLPGDD
                     LARYDLDQPALKLRLDNEELLFGTHNPLDSRQYVAYEGAVYLLPAAYAELAATQIVEM
                     LDKSLLATTEQITGFDFSRLEQWEPTGLRLDHENGQWKVSIAAAKPVQKEIDDWFSEN
                     WQNLTALSVEPYQPDARAKHPTFEIKLKNGKTIHVEKLRESPELQLGRPDEGLIYHFA
                     QDTGFVILNPPIGFKPQ"
     gene            2121..2933
                     /gene="mutM"
                     /locus_tag="PKW44_02820"
     CDS             2121..2933
                     /gene="mutM"
                     /locus_tag="PKW44_02820"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HQR50555.1"
                     /translation="MPELPEVETTRRGLLPLLGQTVRSATVRNAALRWPVPEHLSCIL
                     NGQRLLDLQRRAKYILARFEGGTLLLHLGMSGRLCLLAKAEPPGKHDHVDIVFDSGTV
                     LRLRDPRRFGAVLWIAGDPLQHPLLKQLGPEPLESGFDGDYLYRETRSRSAPIKTVIM
                     DAHLVVGVGNIYASESLFRARISPKTAARKLSRKRCERLAQEIQATLQEALKAGGSSL
                     RDFFGADGNPGYFQQSYFVYGRTGEPCRVCGTPIKTICQGQRSTFYCPHCQR"
     gene            3032..3874
                     /gene="ppk2"
                     /locus_tag="PKW44_02825"
     CDS             3032..3874
                     /gene="ppk2"
                     /locus_tag="PKW44_02825"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HQR50556.1"
                     /translation="MAKTRKKVKHADSDEVKGLPEAKGKLKAKDYDEELKRLHVELVK
                     LQEWVVHKGLKVCIVFEGRDGAGKGGTIKALTERVSPRVFRVIALPAPNDREKSQMYV
                     QRYLPHLPAAGEVVIWDRSWYNRAGVERVMGFTDEAVVKRFLELTPAFEKMMVESGII
                     LIKYWLEVSEEEQTRRLTARIDDGRKIWKLSPMDLKSFSRWYDYSRARDDMFAKTDTS
                     WAPWFVANSDDKKRARLNIISHLLSRIPYEEAPREKVKLPKRQKPGDYVEPNYPYKFV
                     PEKF"
     gene            complement(3884..5596)
                     /locus_tag="PKW44_02830"
     CDS             complement(3884..5596)
                     /locus_tag="PKW44_02830"
                     /inference="COORDINATES: protein motif:HMM:TIGR00815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HQR50557.1"
                     /translation="MLANWFPLLRDLRQYRKEWLSSDLIAGLSVAAVQIPTAMAYADL
                     AGLPPQVGLYASILPVMVYALFASSRQLIVGPDAATCAMIAALLMPIAQGNPVYYLQL
                     MAGLTITTGLLMVIGGSTRMGFFVNFFSRPVLVGYLNGIAISIIAGQLGKILGIQITH
                     RDFGPALLELFRHLEQTHLLSLGLGAMTLVLLVLVQRYAPRAPAALIALAVAGAVAWG
                     LDIAPHGVALVGAMPSSLPSFALPGLGYHGVQAIFMDAVALLVVSFSSGMLTARSFAA
                     RNGYSINADQEMRAIGFANIISGLSGGFAVTGADSRTAVNNASGGKTQLVSLVAALAT
                     AAVILFLTAPLGYLPITALGAVLVISAWGLLDIVSWKELFRISRFEFGLSLLTTVGVL
                     TIGVLPGVVLAILLAIVHVLKKIYQPQEAILGQVPGLDGYNDIALSPEARTIPGVVIY
                     RFESPLLFFNADLFKSRLHALAEQSGAPHTLVLSLEGITQMDVTGLQALFDAHDELKR
                     KGIRLLLARPKRYMRKYARTAHAVDKIGQENIFFSIRSAVKSALATAPLAEPPASPKD
                     QPVA"
     gene            complement(5602..7104)
                     /locus_tag="PKW44_02835"
     CDS             complement(5602..7104)
                     /locus_tag="PKW44_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018230947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HQR50558.1"
                     /translation="MLLWFVILYLMISVGIGLWAATRVHSAKDFAIAGRSLPFPIVMA
                     TVFATWFGAESIFGISATFLKDGLGGVVADPFGSSLCLVLAGILFSRYLYRLNIITLG
                     DFYRMRYNRAVEIITTLCIVISYLGWVAAQIKALGLLFYTVTDGGISQDAGMMLGMML
                     VLTYTVFGGMFSVAILDFVQMLVMLGGLLFIAVIVAGKVDGGTAAVITSAASSGKFKF
                     FPDPDPWLWLTFAGTAVTMMFGSIPQQDVFQRISSAKSARIALWGSVAGASLYFVFTF
                     VPMFIAYAGTMIDPAALGSLVETDAELIVPTLVTTHTPLISQVLFYGAVLSAIMSTAS
                     ATLLAPSVTFSENILRGAFPGIGDHRFLLMMRGSLVVFSGMVMAFALNSDVSIFKMVE
                     NAYKITLAGAFVPLVCGILWRRATTQGALMAIFGGLLSWIMIEVMIGEASPVPPQLIG
                     LGISAIGMVAGSLLPQWVGHKHPQPPGFLHEHAAHVHAEAHTHHPSGAGR"
     gene            complement(7142..8698)
                     /gene="ubiB"
                     /locus_tag="PKW44_02840"
     CDS             complement(7142..8698)
                     /gene="ubiB"
                     /locus_tag="PKW44_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HQR50559.1"
                     /translation="MRLLRFVKIIFVTLRFGLDEFILGHARLQGLNTLVRGLLFWRHF
                     SQPRGLRLRLALEKLGPIFVKFGQMLSTRRDLIPLDIADELARLQDQVPPFPAEQVLE
                     VVLQAYGKAAQEVFAEFDPLPTASASVAQVHFARLLDGTPVAVKILRPGIGPVIEKDL
                     ALLDTAALLMEKLWSDGKRLKPREVVAEFSRHVHQELDLMLEAANCSQLHRNFADGRL
                     LLVPEVHWDWCREQVMVMQRMQGTPISQVARLRELGVDIPKLARDGVEIFFTQVFRDG
                     FFHADMHPGNILVATSEEQRGRYVALDFGIMGTLGDSDKQYLARNFLAFFRRDYKEVA
                     LAHIESGWAPKDTPADEFESAIRAVCEPIFDKPLQDISFGRVLLRLFQTSRHFGVEIQ
                     PQLVMLQKTLLNIEGLGRELDPELDLWKTAKPYLERWVSEQMGWRGALEALQQEAPHW
                     GRILPQLPRLMSEYLQRDDFAQLADQLHRLVQEQRKRSRWLRWGVTALMVLALLQVVG
                     LMLLLGLVPS"
     gene            complement(8698..9267)
                     /locus_tag="PKW44_02845"
     CDS             complement(8698..9267)
                     /locus_tag="PKW44_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR50560.1"
                     /translation="MKYSAYALNHLLRQNSQAQEKLRVYAGKVVRIALPPLQTTLIIL
                     EDGLFAPVLTDATVDAEIGLPPSAALRVLLDPASAASLADLQGDTELAAAVGKVLREL
                     RWEYEEDLSRVIGDIPAHQLTQAGARIKHEFGRQALSLAGMFADYWLEEQPLIAKRRH
                     LDLFAKEVDALRDDVERLEKRLSKLEKSA"
     gene            complement(9264..10001)
                     /gene="ubiE"
                     /locus_tag="PKW44_02850"
     CDS             complement(9264..10001)
                     /gene="ubiE"
                     /locus_tag="PKW44_02850"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HQR50561.1"
                     /translation="MSENTTHFGYQTVAEAEKAKKVGEVFHSVANKYDLMNDVMSAGL
                     HRLWKRYTVDTSGVRPGQRVLDIAGGSGDLSRLFARKVGASGQVVLTDINASMLGVGR
                     DRMLDQGLPVPATQCDAEKLPFPANYFDCVSVAFGLRNMTHKDLALKEMLRVLKPGGK
                     LLVLEFSQVWKPLQPAYDAYSFKLLPLMGKLLAQDADSYRYLAESIRMHPDQETLKQM
                     LLDAGFGRVDYHNLTGGVVALHKGWKY"
     gene            complement(9998..10378)
                     /locus_tag="PKW44_02855"
     CDS             complement(9998..10378)
                     /locus_tag="PKW44_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019897576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HQR50562.1"
                     /translation="MSGLTTKSPRPTDIRLHQLSRRLEISFDDGAQFSLPCEYLRVYS
                     PSAEVRGHGAGQEVLQTGKEEVNIVGIEPVGLYAVKLTFSDAHDTGLYSWDYLYDLGV
                     NQQALWQSYLDRLAAAGIARKEIA"
     gene            10531..11862
                     /gene="miaB"
                     /locus_tag="PKW44_02860"
     CDS             10531..11862
                     /gene="miaB"
                     /locus_tag="PKW44_02860"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019897493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HQR50563.1"
                     /translation="MSTPKKVFIKTFGCQMNEYDSSRMADLMQAADGMTQTDNPEEAD
                     VILINTCSVREKPQEKVFSQLGQYRALKKNNPDLVIGVGGCVASQEGDVIVQRAPFVD
                     VVFGPQTLHRLPQMIAEKRASGRSQVDISFPEIEKFDRLPPPRVEGATAFLSIMEGCS
                     KYCTFCVVPYTRGEEVSRPFDDVLTEAAQLVQQGVKEITLLGQNVNAYRGAMTTGEVA
                     DLGLLLEYLHEIPGIERLRYTTSHPNEMTPALIEAYAKLPKLVSHLHLPVQAGSDRIL
                     MAMKRNYTALQYKSVIRKLRAARPDLHLSSDFIIGFPGETDADFEATMKLIRDVGFDY
                     SFSFIYSPRPGTGASYMEDDTPHEVKVARLEHLQSLLEAQGFENSRKMVGTVQRVLVE
                     GHSRKDAHQLAGRTDNNRIVNFDGPAELVHQFTTVRITEALPHSLRGELVQ"
     gene            11862..>12464
                     /locus_tag="PKW44_02865"
     CDS             11862..>12464
                     /locus_tag="PKW44_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HQR50564.1"
                     /translation="MEVDFIPEDNHRLANLCGALDENLKQIEDALEVGIARRGGHFRL
                     SGETHNMQLAAQLLQDFYERARKPISLEDVQLGLVEVGKLSPESISTASMPVLMTRRT
                     DLHGRTPRQIEYLQQIQEYDITFGIGPAGTGKTYLAVASAVDALTRDRVKRIVLVRPA
                     VEAGERLGVLPADLAQKVDPYLRPLYDALYDLMGFYTVPKM"
BASE COUNT         2650 a         3879 c         3773 g         2162 t
ORIGIN      
        1 aggcaaatcc cccgagtgga ttgtgaaatc cgcggtgagt tacggcgagt ccatcgggct
       61 gccgttcgtg ccggagagtt tctgggcgaa gtcggacctc tacgaactgc cgccggacgc
      121 gaagcgcaaa aagaacaccc acgccttcat cgcccgctac cagcgcgcca agtccgacat
      181 caacctggca ttcgtcaata cctccaagga gccaaagcgc gcccaggaag ccgggatcag
      241 caaagacggc gaaatcgtgg tggaatacga gaagcgcagc gagcatcttg cgccccccta
      301 tgctgaacgc gacctgacca acctgctggt gcgcctggca cgctcgcaga cacgtgccat
      361 catgttcctg gacggccatg gcgaacgcag cctgattggc gagaaaaacc acgacctggg
      421 cgaattcggt catcaactgg gcaagaaggg cttcaagttg gccaaccccg acctcaccgt
      481 tgcccaaggg gtgccgcgca gcggcgccat gctggtgatc gccggcccgc aggttgatgt
      541 ttccccggca gaagtcgaca agatcatggg ctatgtgcat agcggcggca acctgctgtg
      601 gctgatcgac caggaatcgc tgcacggctt gcagccgttg gccgaatatc tcggcctggt
      661 gctgacacca gggatcgcgg tggatccgac ttcatcgagc aacgggctcg acccgcgcac
      721 cgtggtcggc cgccaataca ccgagcatcc catcaccaag gacttcagcc tgctgactgc
      781 gttcccggaa tcgcgccaga tcgaggtgaa agatgccagc ggggactggg aggtcacccg
      841 gctgatttcg gtagcacaga acggctggct ggaaaccggc aagccggagg ccaaaatcga
      901 gttcgacgcc aaacgcgaca ttccgggccc cgttaacatt gccgtcgcca tgcaacgcaa
      961 ggccgaaaat gtcacccagc gcgtggtggt gatcggcaat ggcagtttcc tggccaacgc
     1021 ttttgtcgcc agtggcggca atctcgacct tggcatcaac attgtcaatt ggctggccgg
     1081 tgacgacaaa ctgatcacca tccagcccaa acccctgaag gatgtcaatg tttcactctc
     1141 tcccactcag ggaacgttga tctttggtgg attcgtactg gtgctgccac tggccttcct
     1201 ggtgattggc atcgccatct ggcgacggag acgcacgcga tgaaatcgcg ctggctgctt
     1261 aatattgcct tgctgctgct ggtggcgggg atcgcgctgg cgctgtatct ggtgccacag
     1321 caaaaggacg ccacaccgga aaaggtaaag gtgtccagca tcgacccggg cagcgtctcg
     1381 aaaatcagca tcgagtttcc cgccaaagcg ccggtggtgc tggagaagca ggatggctac
     1441 tggcacctgg tgcaacccta caaggcgcgc gccagccagg aactggcgcg gttgatcact
     1501 tccatcctgt ccgcctccag ctcgaacaaa ttacctggcg acgacctcgc ccgctacgat
     1561 ctcgaccagc cggcactcaa gctgcggctg gataacgaag agcttctgtt tggcacccac
     1621 aacccgctgg acagccggca atatgtcgcc tatgagggcg ccgtgtacct gctgcccgcc
     1681 gcatacgccg aacttgccgc cacccagatc gtggaaatgc tcgacaagtc cctgctggcg
     1741 acgacggagc agatcactgg attcgacttc tcgcggctgg agcaatggga gccgaccgga
     1801 ctgcgcctgg atcatgaaaa cggtcagtgg aaggtttcca tcgccgccgc aaagccggtg
     1861 cagaaggaga ttgacgactg gttctccgag aactggcaga acctcaccgc cttgtcggtc
     1921 gagccctacc agccggatgc aagggccaaa cacccaacgt tcgagatcaa gctgaaaaac
     1981 ggcaagacca ttcacgtcga gaagctgcgc gagtcgcccg aactgcaact ggggcgaccg
     2041 gatgaagggt tgatttacca ctttgcccag gataccggat tcgtcattct gaacccgccg
     2101 ataggtttca aaccacagta atgccagaac tgcccgaggt cgaaacgacg cggcggggcc
     2161 tgctcccgct actcgggcaa acagtgcggt cagccacggt gcgcaatgcc gcgctgcgct
     2221 ggccggttcc cgagcatcta tcctgcatcc tcaacggcca gcgcctgctc gacctgcagc
     2281 gccgcgccaa atacattctg gcccgctttg aaggcggcac cctgctgctg catctcggca
     2341 tgtccggccg attgtgcctg ctggcaaaag ccgagccgcc gggcaagcac gaccacgtgg
     2401 atatcgtgtt cgacagcggc accgtgctgc ggctgcgcga cccacgccgt tttggcgccg
     2461 tgctgtggat cgcgggcgac ccgctgcaac atcccttgct caagcaactg ggtccggagc
     2521 ccctggaatc cggcttcgac ggcgattatc tctatcgcga aacccgcagc cgcagcgccc
     2581 cgatcaagac cgtcatcatg gacgcgcatc tggtagtggg ggtaggcaac atctacgcca
     2641 gcgaatcgct gttccgtgcc cgcatcagcc cgaaaacagc agccagaaag ttaagccgga
     2701 aacgctgcga gcggctggcg caggaaattc aggccacgtt gcaggaagcg ctcaaggctg
     2761 gcggcagcag tctgcgcgat tttttcggcg cggatggcaa ccccggttat ttccagcaga
     2821 gctatttcgt ctacggccgc accggtgaac cctgccgcgt gtgcggcacg cccatcaaaa
     2881 ccatatgcca agggcaacgc tcgacattct attgcccgca ttgccagcgc tgataccaac
     2941 gcggaataga cataatgtct acagggtgtt aggcttttgc caaaccaagc actagtgtag
     3001 tgaccggcct catttgataa ggagacgaga aatggctaaa accagaaaaa aagtgaaaca
     3061 cgccgattcg gacgaagtga aaggccttcc cgaagccaag ggcaagttga aagccaagga
     3121 ctatgacgag gaactgaaac gcctgcatgt cgaactggtc aaactgcagg aatgggtggt
     3181 ccataaaggc ctcaaggtgt gcatcgtgtt cgagggccgc gatggcgcag gcaagggcgg
     3241 aacgatcaag gcactcaccg aacgggtgag tccgcgcgtg ttccgcgtga tcgccctgcc
     3301 ggcgccgaac gaccgcgaga aaagccagat gtacgtgcag cgctacctcc ctcacctccc
     3361 tgccgcgggc gaagtggtga tctgggaccg cagctggtac aaccgcgcag gcgtcgagcg
     3421 ggtgatgggt ttcaccgacg aagccgttgt caagcgcttt ctggaactca cgccggcctt
     3481 cgagaagatg atggtcgaga gcggcatcat cctgatcaag tactggctgg aagtcagcga
     3541 ggaagagcag acccgccgcc tcaccgcccg catcgacgac ggccgcaaga tctggaagct
     3601 gtcgccgatg gacctcaagt cgttcagccg ctggtatgac tattcccgcg cccgcgacga
     3661 catgttcgcc aagaccgaca cctcctgggc gccgtggttc gtggcgaact ccgacgacaa
     3721 gaagcgcgcg cggctgaata tcatcagcca tctgctcagc cggattccct acgaggaagc
     3781 gccgcgggaa aaggtcaagc tgccgaaacg gcaaaaaccg ggcgattacg tcgagcccaa
     3841 ctatccgtac aagttcgttc ccgagaagtt ctgacggaac cggtcaggct accggctggt
     3901 ccttggggct ggccggcggc tcggcgaggg gtgccgtggc cagggctgac ttgaccgccg
     3961 agcggatgga aaagaagatg ttttcctggc cgatcttgtc caccgcgtgc gccgtccgcg
     4021 catatttgcg catgtagcgt ttggggcgcg ccagcagcag gcgtatgccc ttgcgcttca
     4081 attcatcgtg ggcgtcgaac agcgcctgta gtccggtaac atccatctgc gtgattcctt
     4141 ccagcgacag caccaaggta tgcggcgcgc cgctctgttc agccagagca tgcaggcgtg
     4201 acttgaacaa atcggcgttg aagaacagca gcggactttc gaagcggtag atgacgacgc
     4261 cgggaatggt tcgcgcctcc ggtgagagcg cgatgtcgtt gtagccgtcc agccccggca
     4321 cctgcccgag aatggcctcc tgcggctggt agattttctt cagcacgtgg acaatcgcca
     4381 gcaggatggc cagcaccacg cccggtagca cgcctatggt cagcaccccc accgtggtca
     4441 gcagcgacag gccgaactcg aagcggctga tgcggaacaa ctccttccag gacacgatgt
     4501 ccagcaatcc ccatgcggaa atcaccagca cggcgcccag cgcggtaatc ggcagatagc
     4561 ctagcggcgc cgtcaggaac aggatgacgg ctgccgtggc cagcgccgcc accagcgaaa
     4621 ccagctgggt cttgccgccg ctggcattgt tgaccgcggt acgcgaatcg gcgccggtca
     4681 ccgcaaagcc gcccgacaaa ccggaaataa tgttggcgaa gccgatggcg cgcatttcct
     4741 ggtcggcatt gatgctgtag ccgttgcgcg cggcgaagct gcgcgccgtc aacatgccgc
     4801 tggaaaagct caccaccagc aacgccaccg catccatgaa aatggcctgg acgccgtggt
     4861 aacccaggcc aggcagcgcg aacgaaggta ggctggaagg catggcgccg accagggcca
     4921 ccccgtgcgg cgcaatgtcg aggccccaag ccacggcgcc ggccacggcc agcgcgatca
     4981 gcgccgccgg tgcgcgcggc gcgtagcgct gaaccagcac cagcaacacc agggtcatgg
     5041 cgcccagccc aaggctcagc agatgggtct gttccagatg cctgaacagt tccagcaggg
     5101 ccgggccgaa atcccggtgc gtgatctgga tgccgaggat tttgcccagt tgcccggcga
     5161 tgatgctgat ggcgatgccg ttgaggtagc cgaccagcac cggccggctg aaaaagttga
     5221 cgaagaatcc catgcgggtg gagccgccga tgaccatcag caagcccgtg gtgatggtca
     5281 accccgccat gagttgcagg taatagaccg ggttgccctg cgcgattggc atcagcagcg
     5341 ccgcaatcat ggcgcaggtc gccgcatccg gccccacgat caactggcgc gaggaagcga
     5401 acagcgcata taccatcacc ggcaatatgc tggcgtacaa gcctacctgc ggcgggagcc
     5461 cggccaggtc ggcgtaggcc atcgccgtgg ggatctgtac cgcagccacc gacaggccgg
     5521 cgatcaagtc gctcgacaac cattctttcc tgtattggcg aaggtcacgc agcagcggga
     5581 accagtttgc cagcatggcg gctacctacc cgcgccggaa ggatgatgcg tatgggcctc
     5641 cgcatggaca tgtgcagcat gctcatgcaa gaatcccggc ggctgcggat gcttgtgccc
     5701 gacccactgc ggcagcaggg agccggccac catgccgatg gcactgatgc ccagaccaat
     5761 cagttgcggc ggcaccggac tggcttcgcc gatcatcact tcgatcatga tccaggacag
     5821 caggccgcca aaaatggcca tcaacgcccc ctgcgtggta gcgcggcgcc acaggatacc
     5881 gcatacgagg ggaacgaagg cgccggccag ggtaatcttg taggcgttct cgaccatctt
     5941 gaagatcgag acatcggaat tcagcgcgaa ggccatcacc ataccggaaa acactaccag
     6001 cgagccgcgc atcatcagca gaaagcggtg gtcgccgatg ccgggaaagg cgcctctcag
     6061 gatgttctcg gaaaaggtca ccgaaggtgc cagcaaggta gccgacgcgg tgctcatgat
     6121 ggccgagagc accgcgccat agaacagcac ctgcgagatc agcggcgtat gggtggtcac
     6181 cagcgtgggc acaattagtt cggcgtccgt ttcgaccagg ctaccgagtg ctgccggatc
     6241 gatcatggtg ccagcgtagg cgatgaacat gggcacgaaa gtaaacacga aatacaacga
     6301 ggcgcccgcc acagagcccc acagggcaat cctggcgctt tttgccgagg atatacgctg
     6361 aaacacgtcc tgctgcggaa tcgagccgaa catcatggtc accgcggtac ccgcaaaggt
     6421 cagccacagc catggatccg gatcgggaaa aaacttgaac ttgccgctcg acgccgcact
     6481 agtgattacg gcggcggttc cgccatcaac cttaccggcg acgattacag cgataaacag
     6541 caggccaccc agcatgacga gcatctgcac aaagtcgaga atggcgaccg agaacatacc
     6601 gccgaatacg gtataggtca gcaccagcat catgcccagc atcatgcccg catcctggct
     6661 gatgccgccg tcggtgacgg tatagaacag cagaccgagc gccttgatct gcgccgcgac
     6721 ccagcccaag taggagatta cgatgcagag ggtggtgatg atttccacgg cgcggttata
     6781 gcgcatgcgg tagaagtcgc ccaaggtgat gatattcagc cggtacagat agcgcgagaa
     6841 caggatgccg gcaagcacca agcacaaaga ggaaccaaaa ggatcggcca ccacgccgcc
     6901 gaggccatcc ttcaggaagg tggcggagat gccgaaaatg gattcggcgc caaaccaggt
     6961 ggcgaacacc gtcgccatca cgatggggaa cggcagcgag cgcccggcga tggcgaagtc
     7021 cttggcactg tgcacgcggg tcgcggccca cagaccgatg ccgacggaaa tcatgagata
     7081 gagaatgacg aaccagagca gcacgtgacc cccagatgcg tgtttgaaca ggtctgttat
     7141 tctagcttgg aaccaagccc agcaacagca tcaggccgac gacctgaagc agggccaata
     7201 ccatcagcgc ggtcacgccc caacgcagcc agcgcgacct tttgcgttgc tcctgcacca
     7261 gccggtgcaa ctgatcggcc agttgcgcga aatcgtcgcg ttgcaggtac tcgctcatca
     7321 ggcgcggcaa ttgtggcagg attctgcccc agtgcggcgc ctcctgttgc agcgcctcca
     7381 gtgcaccgcg ccagcccatc tgttccgaca cccagcgttc cagataaggc ttggcagttt
     7441 tccacaaatc cagttccggg tcgagttcgc gccccagtcc ttcgatattg agcagcgtct
     7501 tttgcagcat caccagttgc ggctggattt ccacgccgaa gtggcgcgag gtctggaaca
     7561 ggcgcagcag cacgcggccg aaggagatgt cttgcagcgg cttgtcgaaa atgggctcgc
     7621 acacagcgcg gatggcagat tcaaattcgt ccgctggcgt gtccttgggt gcccagccgg
     7681 attcgatatg ggccagggcg acctccttgt aatcgcgacg gaaaaaggca aggaagttgc
     7741 gcgccaggta ttgcttgtcg ctgtcgccca acgtacccat gatgccaaaa tccagcgcca
     7801 cgtagcggcc gcgctgttct tcactggtcg ccaccaggat gttgccgggg tgcatgtctg
     7861 cgtggaaaaa gccgtcgcgg aacacctggg tgaaaaagat ttccacgccg tcgcgcgcca
     7921 gttttggaat atccacgccc aactcgcgca gccgtgccac ctggctgatc ggcgtgcctt
     7981 gcatgcgctg catcaccatg acctgttcgc ggcaccagtc ccagtgcact tccggtacca
     8041 acagcaggcg tccatcagcg aagttgcgat gcaactggct gcaattggcg gcttccagca
     8101 tcaggtccag ttcctggtgg acgtgcctgg agaattccgc caccacttcg cgcggcttga
     8161 ggcgcttgcc gtccgaccac agtttctcca tcagcagcgc ggcagtatcg agcagtgcca
     8221 ggtctttttc gatgacgggg ccgatgccgg gacgcaggat ttttaccgcc accggcgtgc
     8281 catccagcaa cctggcgaaa tgcacctgtg ccaccgaagc gctagccgtc ggcagcggat
     8341 cgaattcggc gaatacctcc tgcgccgcct tgccgtaggc ctgcaacacc acctccagca
     8401 cttgctcggc gggaaacggc ggcacctggt cctgcaatct tgccagttcg tcggcgatgt
     8461 ccagcggaat caggtcgcgc cgggtggaca gcatctggcc gaacttgacg aaaatcggcc
     8521 ccagcttttc cagtgccagc cgcagacgca agccgcgcgg ctggctgaaa tggcgccaga
     8581 acagcaggcc gcgcaccagc gtgttcaggc cctgcagacg ggcatggccg agaatgaatt
     8641 cgtcgagacc gaaacgcaag gtgacgaaaa tgattttgac gaaacgcagc aaccgcatca
     8701 ggcagacttt tccaacttcg acagacgctt ctcaagccgt tcgacatcgt cgcgcagggc
     8761 gtcgacttct ttggcgaaca gatcgaggtg gcggcgcttg gcgatcagcg gctgctcctc
     8821 cagccagtaa tcggcgaaca tgccagccag ggatagtgcc tgacggccga attcgtgctt
     8881 gattctggcg ccggcctggg tcagttggtg cgcgggaatg tcgccgatga cgcgactcag
     8941 gtcttcttca tattcccagc gcaattcgcg caacaccttg ccgacggcag cggccaactc
     9001 ggtgtcgccc tgcagatcgg cgaggctggc ggctgaggcg ggatcgagca gcacgcgcag
     9061 agcggcagag ggtggcaggc cgatctcggc atctacggtg gcatctgtca gcaccggcgc
     9121 gaacaggccg tcttccagga tgatcaaagt ggtttgcagc ggcggcagcg cgatacgtac
     9181 caccttgccg gcgtagacgc gcagtttttc ctgcgcctgg ctattctggc gcagcaggtg
     9241 gttgagggcg taagcggagt atttcaatac ttccaacctt tgtgcaaggc caccacgccg
     9301 ccggtcaggt tgtggtaatc caccctgccg aagccggcat ccagcagcat ctgcttgagc
     9361 gtttcctggt ccgggtgcat gcggatggat tcggccaggt agcggtagct gtccgcatcc
     9421 tgcgccagca gtttgcccat cagcggcagc agcttgaaag aataagcatc gtaggccggc
     9481 tgcagcggct tccatacctg cgagaattcc agcaccagca gtttgccgcc cggcttaagc
     9541 acgcgcagca tctccttcag cgccagatcc ttgtgcgtca tgttgcgcag gccgaaggcg
     9601 acgctgacgc agtcgaaata attggccggg aacggcagct tttcggcatc gcactgcgtg
     9661 gctggcaccg gcaagccttg gtccagcatg cggtcgcgcc ccacgcctag catggaagca
     9721 ttgatgtcgg tcagcaccac ctggccacta gcccccactt tgcgagcgaa cagtcgcgac
     9781 aagtcgccgc tgccgccggc gatgtccagc acgcgctggc ccggtcgcac gccgctggta
     9841 tccacggtgt agcgcttcca caggcggtgc agcccggccg acatgacgtc gttcatcagg
     9901 tcgtatttgt tggcgacgga atggaacact tcgccgactt ttttggcctt ttcagcctcg
     9961 gcgacggtct ggtagccgaa atgggtagtg ttttcgctca tgcgatttcc ttgcgtgcga
    10021 tgccggcagc ggccagcctg tccaggtaac tttgccagag tgcttgctgg ttgacaccga
    10081 ggtcgtacag ataatcccag gaatacaagc cggtgtcgtg cgcgtcggag aacgtcagct
    10141 tgaccgcata gagaccgacc ggctcgatac cgacgatgtt gacttcctcc ttgccggttt
    10201 gcagcacttc ctgtccggcg ccgtggccgc gcacctcagc cgagggcgaa tacacgcgca
    10261 ggtattcgca aggcaggctg aattgggcgc cgtcgtcgaa gctgatctca agccggcgcg
    10321 aaagctggtg cagacggata tcggtaggac ggggagattt ggtggtgagg ccgctcatga
    10381 gcatgtcaac tacattacat aatggcggat gatagcagaa tatgcctgcc cgcaagttgc
    10441 catgcgcccg cgcactcctc tataatgcgc ccttagctta ctgttttttc aaagatattt
    10501 tgcaaatcat ccgccccggc gcagcctgtc atgagcacgc ctaaaaaagt tttcatcaag
    10561 accttcggct gccagatgaa cgagtacgac tcgtcgcgca tggcggacct gatgcaggct
    10621 gcggacggca tgacgcagac cgacaatccg gaggaagccg acgtcattct gatcaacacc
    10681 tgctcggtgc gcgagaagcc gcaggagaag gtgttcagcc agctcggcca gtaccgtgcg
    10741 ctgaagaaga acaatcccga tctggtgatc ggcgtcggcg gctgcgtggc cagccaggag
    10801 ggcgatgtca tcgtccagcg cgcgccgttt gtggacgtgg tgttcggccc gcaaaccttg
    10861 caccgcctgc cgcagatgat cgccgaaaag cgcgcttccg gccgctcgca ggtggacatc
    10921 agcttcccgg aaatcgagaa gttcgaccgc ctgccgccgc cgcgcgtgga aggtgccacc
    10981 gccttccttt ccatcatgga aggctgcagc aagtactgca ccttctgcgt ggtgccctat
    11041 acccgcggcg aggaagtctc gcgcccgttc gacgacgtgc tcaccgaagc ggcgcaactg
    11101 gtgcagcaag gcgtgaagga aatcaccctg ctcggccaga acgtcaacgc ctatcgcggg
    11161 gccatgacta ccggcgaggt ggctgacctt ggcctgctgc tggaatatct gcacgagata
    11221 ccgggcattg agcgcctgcg ctacaccacc tcgcacccca acgagatgac accggcgctg
    11281 atcgaggcct acgccaagct gcccaagctg gtgtcgcatt tgcacctgcc ggtacaggcc
    11341 ggctccgacc gcatcctgat ggcgatgaag cgcaactaca ccgcgctgca atataaatcg
    11401 gtgatccgca agctgcgcgc ggcgcgtccc gacctgcacc tatcgtcgga cttcatcatc
    11461 ggtttccccg gagaaaccga tgccgatttc gaggccacca tgaagctgat ccgggacgtc
    11521 ggcttcgact acagcttcag cttcatctac agcccgcgcc ccggcaccgg cgcctcctat
    11581 atggaagacg ataccccgca cgaggtcaag gtggcgcggc ttgagcacct gcagtcactg
    11641 ttggaagcgc aggggttcga gaacagccgg aagatggtcg gcactgtcca aagagtgctg
    11701 gtcgaaggcc actccaggaa agacgcacac caacttgccg gacgcaccga caacaatcgc
    11761 atcgtcaatt tcgacggccc ggcggaactg gtgcaccagt tcaccaccgt acgcattacc
    11821 gaagcactac cgcatagtct gcgtggcgaa ctggtgcagt gatggaagtc gatttcatcc
    11881 ccgaagacaa ccaccgcctc gccaacctct gcggcgcgct ggacgaaaac ctgaagcaga
    11941 tcgaggatgc gctggaagtc ggcatcgcgc ggcgtggtgg gcatttccgc ctgtccggcg
    12001 agacgcacaa catgcagctg gcggcacagt tgctgcagga tttctacgaa cgcgccagga
    12061 agccgatcag cctggaggac gtgcaactag gcttggtgga agtcggcaag ctcagcccgg
    12121 aatccatcag caccgcctcc atgccggtgc tgatgacgcg acgcaccgac ctgcatggcc
    12181 gcacgccgcg ccagatcgag tatctgcagc agatccagga atacgacatc accttcggca
    12241 tcggcccggc cggcaccggc aagacttacc tcgccgtggc cagcgcggta gatgccctga
    12301 cgcgcgaccg ggtgaagcgc atcgtgctgg tccgccccgc agtcgaagcc ggcgagcgct
    12361 tgggcgtgct gcccgccgac ctggcgcaga aggtcgaccc ctacctgcgt ccgctctacg
    12421 acgcactgta cgatctgatg ggcttctaca cggtacccaa gatg
//
LOCUS       DAOMFA010000032        12358 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_497_length_12358_cov_6.067951, whole genome shotgun sequence.
ACCESSION   DAOMFA010000032 DAOMFA010000000
VERSION     DAOMFA010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 12358)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12358)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12358
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_497_length_12358_cov_6.067951"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(274..744)
                     /gene="nusB"
                     /locus_tag="PKW44_02870"
     CDS             complement(274..744)
                     /gene="nusB"
                     /locus_tag="PKW44_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HQR50565.1"
                     /translation="MTSETKPAKKPSRNRRKSRELVLKGLYRLFISGNSSEEILRDLA
                     EDDDYAHADEEYFRVLLRGVAEKVGQLDQQIGPLLDRPVGELSPIEHAILCISGYELM
                     HDPSIPYRVAINEGVELAKRYGGTDGYKYVNGVLDKLAAQARPEEFRSGRKNSG"
     gene            complement(744..1193)
                     /gene="ribH"
                     /locus_tag="PKW44_02875"
     CDS             complement(744..1193)
                     /gene="ribH"
                     /locus_tag="PKW44_02875"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HQR50566.1"
                     /translation="MKEIERNLDGSGLRVGIVLSRFNNEIGDGLLGACVAELRKLGVA
                     DDAVTLSTVPGALESPLVLRKMAASGKFDALIALGAVIRGETYHFEIVADESARGISQ
                     VQLETGVPVANAILTTEDDDQALARMSVKGAEAAAVAVEMVNLLKRL"
     gene            complement(1201..1758)
                     /locus_tag="PKW44_02880"
     CDS             complement(1201..1758)
                     /locus_tag="PKW44_02880"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HQR50567.1"
                     /translation="MKSSWLLFLTCLLVMPAQAGTATETATALNARVEVAFTPGDDIA
                     GKIITAIDRARRQVLVQAFSFTHDDIARALLDAHRRGVEVRLIADREQTENMARGQVP
                     GLARAGLPIWLDGEHQSAHNKVMVIDAGTPAALLITGSFNFTRAAQYKNAENAVFISG
                     NDALIRTYVRNWQNHLKHSQPLTIH"
     gene            complement(1748..2017)
                     /locus_tag="PKW44_02885"
     CDS             complement(1748..2017)
                     /locus_tag="PKW44_02885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR50568.1"
                     /translation="MKVELMLCATLALPLAVRAELPPPPEEAAKKQEAAQKKARDENS
                     ARAALTRAQNRVAERYHAAHTPPVQKPARSEEAEPIPESEASIEE"
     gene            complement(2014..3030)
                     /locus_tag="PKW44_02890"
     CDS             complement(2014..3030)
                     /locus_tag="PKW44_02890"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit gamma"
                     /protein_id="HQR50569.1"
                     /translation="MTKTQKFLLLIVALLLVVAGSVRQYEEQSRVERDAYTGATPFSL
                     DEGRTAGVKSDAEIKAESGIAGITQSRIRDAGVFQQKEGQLWRTLRNGPVTYFGGWLL
                     VLVPLLILGFYAIYGAMHLHGQPTGRLIQRFSVAERHVHWASAGTFVLLGLSGIVILF
                     GKHLLLPLVGYQAFSWIAVIAKNLHNLAGPLFFVSVALLFRRYVKDNLWHKIDWLWIK
                     DAHNVLRGETHVPSYKFNAAEKAWFWGGVTLLGLVVSLSGFVLDFPVFNQGRHVMQAA
                     NLIHGVCALLFICAAFGHVYMGTIGADGAFQAMKTGMVDETWAKEHHALWYEEEKVKQ
                     GLDK"
     gene            complement(3034..3261)
                     /locus_tag="PKW44_02895"
     CDS             complement(3034..3261)
                     /locus_tag="PKW44_02895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR50570.1"
                     /translation="MMQAARTVLLILLLPALLAGCGEYKQAVDYQDGGYQGKQDGRPW
                     DSERFMHDPSAWRQVLHERNQRQNEYGRTGD"
     gene            complement(3258..3890)
                     /gene="fdh3B"
                     /locus_tag="PKW44_02900"
     CDS             complement(3258..3890)
                     /gene="fdh3B"
                     /locus_tag="PKW44_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008064925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase FDH3 subunit beta"
                     /protein_id="HQR50571.1"
                     /translation="MARMKFICDVDRCIECNACVTACKNEHEVPWGINRRRVITLNDG
                     QPGEKSVSIACMHCTDAPCMAVCPVNCFYKTEDSVVLHDKDICIGCGYCSLACPFDAP
                     QFPNSNPSGKEPGGHATDFFVARGKMDKCTFCAGGPEENGSEAEFDKYGRNRLAEGKL
                     PLCAEMCSTKALLAGDAPVIVAIHESRIETRSYGDQRWGWDIAYSRPEKK"
     gene            complement(3910..6789)
                     /locus_tag="PKW44_02905"
     CDS             complement(3910..6789)
                     /locus_tag="PKW44_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020165591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HQR50572.1"
                     /translation="MLLKKKSPMVTPAPASGFASWLGNTLDRREFLKRSGLTAGAGVA
                     LSQLPFNMIAKAGTSESRTSGPIETRRTVCTHCSVGCSVNAVVQNGVWIRQEPAFDSP
                     INLGAHCAKGASVREHGHGERRLKYPMKMVDGKYQRISWEQALEEISAKLLSIRKESG
                     PDAVYWVGSSKHNNEQAYLLRKFVSFWGSNNVDHQARICHSTTVAGVANTWGYGAMTN
                     SYNDIHNTKAMLFVGSNAAEAHPVAMLHVLHAKEKGARMIVVDPRFTRTAAHAHQYVR
                     LRPGTDIPFVWGVLWHIFQNGWEDQQFIHDRVYGMDDIRQEVANWRPDKVADVTGIPE
                     AEVYQVAKTLHENRPATVIWCMGITQHTVGNANTRALCILELALGNVGVSGGGTNIFR
                     GHDNVQGATDVGPNPESLPGYYGLTEPAWKHWCTVWGVDYEWVKGRYASQVMMEKPGM
                     PVSRWIDGVLEKNEHLDQGPNLRAAIYWGMAANSQTRGAEMKAAMEKLDLLVVIDPYP
                     AAASVMQDRKDNTYLLPAATQFETYGSCTASNRSIQWREQVIEPLFESRADHVIMYAF
                     AKKFGFEQELCVNIKVKKNRQGWDEPSIEDTLREINRGTWSIGYSGQSPERLKLHMKH
                     AHTFDVTTLKASEGPCAGDYYGLPWPCWGTPEMKHPGSPNLYDISVPVAEGGMGFRAN
                     FGVERDGISLLAGDNVTVKGSDLEGGYPEFDHVLLKKLGWWQELTPAEQKAAEGKNWK
                     TDLSGGIQRIAIKHGCAPFGNAKARAVVWNFPDPVPVHREPLYTKRRDLIGKYPTYKD
                     MKTFWRVPTLYQSVQQANKDAALDYPVILTSGRLVEYEGGGEETRSNPWLAELQQEMF
                     CEVNPQDAKAQGVANHDFIWVETPTRARIKVRVLVTERVAPGVAFMPYHFGGWWEGKD
                     LLDKYPEGAAPYVRGEAANTVTTYGYDSVTMMQETKVTLCRFTRA"
     gene            complement(6792..6992)
                     /locus_tag="PKW44_02910"
     CDS             complement(6792..6992)
                     /locus_tag="PKW44_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019917118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase"
                     /protein_id="HQR50573.1"
                     /translation="MEKTNIKRRNFLLGLGLGGMGAAVALLTGKPATAPETEADTAEA
                     PPASKGYRLSQHVQTYYRKARI"
     gene            complement(7022..7636)
                     /locus_tag="PKW44_02915"
     CDS             complement(7022..7636)
                     /locus_tag="PKW44_02915"
                     /inference="COORDINATES: protein motif:HMM:NF014651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD family protein"
                     /protein_id="HQR50574.1"
                     /translation="MNAVAGDLPLTEEDQARAGLYALLARLLYTAPDVPLLRAIAQAD
                     ELAAGSNLAQAWQGLQAASTVMDAEAAAQEYEQVFIGLGQGEVMPYLSWHLTGFLMEE
                     PLARLRDDLATLGLARVAGAGEPEDHMAAICEVMRLLVAGDADSAPASIKQQRAFFDR
                     NLRPWYTKFATQLELAPSANYYRAVAGLLRAYMDVEAQAFGMAG"
     gene            complement(7633..9216)
                     /locus_tag="PKW44_02920"
     CDS             complement(7633..9216)
                     /locus_tag="PKW44_02920"
                     /inference="COORDINATES: protein motif:HMM:NF024585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HQR50575.1"
                     /translation="MSPPVQSVLAAIAAYPAPKPVPVVEYRSAGHVLVIGDTPQALAV
                     ARDLADKLTVSLLLTKPAGATTGTSLPIFYGEIEIITGWLGEFTATWTGGSGNFDLVL
                     NLTANRYFTMHQPPQGYFAPADVATLQQATAEILDAVGEFEKPKFFLYQEKTCAHSRS
                     RLPGCDRCIEVCSTEAISAQGEHIRVEPHLCMGCGACATVCPSGAMQYNFPSVSYWGG
                     KLQAALAAYRTAGGTDACLLLHNPGDGAALVQASALPANVIPLEIFHVASIGLDWLLG
                     ALALGAGRVVILAAGSEAPQYLTALHTQMALGSKILQELGYGEGHFSLIETADAAALQ
                     QALAEEALGSVPQQRAAFRLFDDKRTTLEFCIEHFANHAPTSVPEALALPTGAPFGAV
                     EINGAACTLCLSCVSVCPAAALQDGAGAPQLKFIERNCVQCGLCESACPEDAIHLQPR
                     LLLTPAAKQARVLHEDRPFPCVRCGKPFATTHMVGAILHKLAGHSMFATPEARRRLQM
                     CGDCRVKDTMEAGETMAVK"
     gene            complement(9213..9584)
                     /locus_tag="PKW44_02925"
     CDS             complement(9213..9584)
                     /locus_tag="PKW44_02925"
                     /inference="COORDINATES: protein motif:HMM:NF023176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3306 domain-containing protein"
                     /protein_id="HQR50576.1"
                     /translation="MSKPLSFIRRWSRLKLQTAAEPATPVPTTTPALPAMTELGFRAD
                     FSPFMHPEVDGATRRTALRKLFMTDHYRTMDGLDVYVEDYSHPAELPAELLATLDHAQ
                     AMLAPAEEKTSDPTQEKPELV"
     gene            complement(9659..10231)
                     /locus_tag="PKW44_02930"
     CDS             complement(9659..10231)
                     /locus_tag="PKW44_02930"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HQR50577.1"
                     /translation="MYRCVWAKSPLDIAYHDTEWGVPQHDDRALFEFLVLEGAQAGLS
                     WSTILNKRARYREVFDDFDPQRVARYDDAKVAQLLADPGIVRNRLKIAAAVNNAQRTL
                     QVQQEFGSFDAYLWRFVDGNPIQNAWASWQEVPATTPISDALSKDLARRGFKFIGSTI
                     CYAMMQAVGMVNDHSVDCFRHGEILTMAKS"
     gene            complement(10232..10363)
                     /locus_tag="PKW44_02935"
     CDS             complement(10232..10363)
                     /locus_tag="PKW44_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018229887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxalate:formate antiporter"
                     /protein_id="HQR50578.1"
                     /translation="MENTESQKTENHAVLLVLFWAYVLIPLGWGVFQTFKKALALFH"
     gene            complement(10405..11775)
                     /locus_tag="PKW44_02940"
     CDS             complement(10405..11775)
                     /locus_tag="PKW44_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HQR50579.1"
                     /translation="MSGFFSRERITAGHNYNRWLIPPAALAVHLCIGQAYAFSVFNEP
                     LTRVIGVAESAPDDWKLTTIGWIYSLAIVFLGLSAAFGGKWLEKVGPRRTMFTAACCF
                     GGGFLISAIGIKTHQIWLLYLGYGVLGGCGLGLGYVSPVSTLIKWFPDRRGMATGMAI
                     MGFGGGAMIGAPLGVALMKHFASDTSVGVMQAFVVMGVVYFISMVIGSLAIRIPPADW
                     KPKGWTPPAEAKKMVTKRHVHIDQALRTPQFYLLWLILCLNVTAGIGILGQASVMIQE
                     MFKGNITPEAAAGFVGLLSLFNMGGRFVWSSASDYLGRKNTYFLFFALGTFLYSMLPT
                     FSGEGNIVLFIAASAVILSMYGGGFATIPAYLADIFGTKYVGGIHGRLLTAWSTAGVL
                     GPVLVNYIREYQISQGVAKADAYTTTLYIMAGLLTVGFVCNLLMKEVHEKHYMKHDEV
                     DDPLQG"
     gene            11990..>12358
                     /locus_tag="PKW44_02945"
     CDS             11990..>12358
                     /locus_tag="PKW44_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQR50580.1"
                     /translation="MPANILVVEDEPAIQELLALNLTQAGHNAIRALSVEQAQLLMRE
                     TMPDLIILDWMLPGTSGIDFARRLKSDEYSRGIPIIMLTARGEEADKVRGLEVGADDY
                     VTKPFSPRELNARIKAVLRRR"
BASE COUNT         2213 a         3838 c         3804 g         2503 t
ORIGIN      
        1 tccagcgcca ccagcacaaa tgcgctggat ccgatccatg aactccatgg tggggaaatg
       61 acgcctccgt tcaggatgct catcgccgca tgcgtcaggt tttccccttc acccggcatg
      121 atctggaagc ctttggatag ctggacggat ggcaagcagc gggcgcaatg aaaacgccac
      181 ccctgcgaac ccgtttaact tccagaaaca cctcttcacc tggatcacct tttacggact
      241 gccggcataa aatcggcgct acaacgtcaa ctttcagcca gaatttttcc tgcccgacct
      301 gaattcttcc ggccgcgcct gtgccgccag tttgtccaat accccgttga cgtacttgta
      361 accatcggtg ccaccgtagc gcttggccag ttccacccct tcgttgatcg ccacgcgata
      421 gggaatactt gggtcatgca tcaactcata gccggagata cagaggatgg cgtgttcgat
      481 cggactgagt tcgccgaccg gacggtcgag cagggggccg atctgctgat cgagttgccc
      541 aaccttctct gccacgccgc gcagcaggac acggaaatat tcctcgtcgg catgggcata
      601 atcgtcatcc tcagccaggt cgcgcagaat ttcttcggag ctgttgccgc tgatgaacag
      661 gcgatacagc cctttgagca caagctcgcg tgacttgcgg cggttgcgcg acggcttctt
      721 ggcaggtttg gtttcgctcg tcatcacagg cgcttcagca ggttgaccat ttccactgcc
      781 acggcagcgg cttccgcccc cttgactgac atgcgcgcca gcgcctgatc gtcgtcttcc
      841 gtggtaagga tggcgttggc gaccggtacg ccggtctcca gttgcacttg ggaaatgccg
      901 cgcgcggatt cgtcggcgac gatttcgaaa tggtaagttt cgccacggat gacggcaccc
      961 agtgcaatca gcgcgtcgaa cttgccgctg gcagccatct tgcgtaacac cagtggggat
     1021 tccaatgcgc ctgggacggt ggacagggta acggcgtcat ccgctacccc cagcttgcgc
     1081 aattcggcga cacaggcgcc cagcaggcca tcgccgattt cgttgttgaa acgcgacagc
     1141 acgatgccaa cgcgcaagcc gctaccatcc agattccgtt cgatttcctt catgatgctc
     1201 ctagtgtatc gtcagtggct gcgaatgctt caggtggttc tgccagttac ggacataagt
     1261 ccggatcaag gcatcgttcc cactgataaa aaccgcattc tccgcgtttt tgtactgcgc
     1321 tgcacgagta aagttgaacg agccggtaat caatagcgct gccggcgtcc ctgcatcgat
     1381 caccatgacc ttgttatgcg cgctctggtg ttcgccgtcc agccatatcg gcagtccggc
     1441 gcgcgccagc cctggcactt ggccgcgcgc catgttttcg gtctgctcgc ggtcggcgat
     1501 cagcctgacc tcgaccccgc ggcgatgggc gtcgagcagc gcgcgtgcaa tgtcgtcatg
     1561 ggtgaaggag aacgcctgta ccagcacttg gcgtcgtgcg cgatcaatcg ccgtgattat
     1621 tttaccggca atgtcatcgc cgggcgtaaa cgcgacctct acccgggcat tgagggcagt
     1681 tgcggtctcg gttgcggtgc cggcttgagc cggcattacc agcaggcagg tcaggaatag
     1741 tagccaacta ctcttcaatg ctggcctccg attccgggat cggctcagct tcttcacttc
     1801 tggccggttt ctgcactggc ggcgtgtgtg ccgcatggta gcgttccgcc acccgattct
     1861 gcgctcgcgt caacgccgct ctggcgctgt tttcgtcacg cgctttcttt tgcgccgctt
     1921 cctgcttctt ggccgcttct tccggcggcg gtggcagttc ggcgcggacg gcgagcggca
     1981 acgccagcgt ggcgcatagc attaattcca ccttcatttg tccagcccct gcttcacttt
     2041 ctcttcctcg taccatagcg cgtgatgctc cttggcccag gtctcgtcca ccatgccagt
     2101 cttcatcgcc tggaatgcgc cgtcggcgcc tattgtcccc atatagacgt ggccgaaggc
     2161 ggcgcagatg aacagcaacg cgcacacgcc gtgaatcagg ttggcggcct gcatcacgtg
     2221 gcggccctgg ttgaacaccg ggaagtcgag cacaaagccg gacaggctga ctaccagccc
     2281 cagcaaggtg acgccacccc agaaccaggc cttctcggcg gcattgaact tataggacgg
     2341 cacgtgcgtt tcgccgcgca gcacgttgtg cgcatccttg atccacagcc agtcgatctt
     2401 gtgccacagg ttatccttga catagcgccg gaacagcagc gccacggaaa cgaagaacag
     2461 cggccccgcc aggttgtgca ggttcttggc gatgacggcg atccaggaaa acgcctgata
     2521 gcccaccaac ggcagcagca ggtgcttgcc gaacaaaatg acgatgccgc tcagccccag
     2581 cagcacgaaa gtgccagcac tggcccagtg cacgtggcgc tcggcaacgc tgaagcgctg
     2641 gatcaggcgg ccggtcggct ggccgtgcag atgcatggcg ccgtaaatcg cgtagaagcc
     2701 cagaataagc aatggcacca gcaccagaag ccagccgccg aagtaggtca ccgggccgtt
     2761 gcgcagggtg cgccacaact ggccttcctt ctgctggaac acgccggcat cgcggatgcg
     2821 cgactgcgtg atgccggcga tcccggattc ggccttgatc tcggcgtccg acttcacccc
     2881 ggcagtcctg ccctcgtcca gcgagaacgg cgtggcgccg gtgtaggcgt cacgctcgac
     2941 cctggattgc tcctcgtact gcctgacgct ccctgccacg accagcagca gcgcgacgat
     3001 cagcagcaga aatttttgcg tttttgtcat ggatcaatcc cccgtgcggc cgtattcgtt
     3061 ctggcgctgg ttgcgttcgt gcagcacttg ccgccatgcc gacgggtcgt gcatgaaacg
     3121 ctcgctatcc cagggacgcc catcctgctt accctgatag ccgccatcct ggtagtccac
     3181 tgcctgcttg tactcgccgc agccggccag tagcgcaggc agcagcaaaa tcaataatac
     3241 tgttctcgcg gcttgcatca tttcttctcc ggccggctgt aagcgatgtc ccagccccag
     3301 cgctggtcgc cgtagctgcg tgtttcgatg cggctctcgt ggatggcgac gatcaccggc
     3361 gcatcgcctg ccagcagcgc cttggtcgag cacatctcgg cgcacagcgg cagtttgcct
     3421 tccgccaggc ggttgcgccc atacttgtcg aactccgctt cgctgccgtt ttcttccggg
     3481 ccaccggcgc agaaggtgca cttgtccatc ttgccacggg ccacgaagaa atcagtcgcg
     3541 tgcccgcccg gctccttgcc ggatggattg gaattgggga actgcggtgc gtcgaacggg
     3601 caggccagcg agcagtaacc gcagccgatg cagatgtcct tgtcgtgcag tactacgctg
     3661 tcctcggtct tgtagaagca gttgaccggg cacaccgcca tgcagggcgc atcggtgcag
     3721 tgcatgcagg cgatggacac cgacttctcg ccgggttggc cgtcgttgag ggtaatcact
     3781 cgccgccgat tgatgcccca cggcacctcg tgctcgttct tgcaggccgt gacgcaggcg
     3841 ttgcactcga tgcaacgatc cacgtcacag atgaatttca ttcttgccat aaatcgttcc
     3901 tctacccgtt caagcgcgcg tgaagcggca caacgtgacc ttggtttcct gcatcatggt
     3961 gaccgaatcg taaccgtagg tcgtgaccgt attggccgcc tcgccgcgca cgtagggcgc
     4021 ggcgccctcc gggtacttgt ccagcaaatc cttgccttcc caccagccgc cgaagtgata
     4081 gggcatgaag gctacgcccg gtgctacgcg ctccgttacc aggacgcgca ccttgatgcg
     4141 cgccctggtc ggcgtttcca cccagatgaa gtcatggttc gccacgccct gcgctttggc
     4201 gtcctgtggg ttgacctcgc agaacatttc ctgctgcagt tcggccagcc aggggttgga
     4261 gcgggtttcc tcgccgccgc cctcgtattc caccaggcgg ccgctggtga ggatcaccgg
     4321 gtagtccagc gcggcgtcct tgttggcctg ctgcactgac tggtagagcg tcggcacgcg
     4381 ccagaaggtc ttcatgtcct tgtacgtcgg atactttccg atcaggtcgc ggcgtttggt
     4441 gtagagcggc tcgcggtgca ccggcaccgg gtcggggaag ttccacacca cggcgcgtgc
     4501 cttggcattg ccgaatggcg cgcagccgtg cttgatcgct atgcgctgaa tgccgccgga
     4561 aaggtcggtc ttccagtttt tgccttcggc ggccttctgc tcggccgggg tcagttcctg
     4621 ccaccagccc agttttttca gcagcacatg gtcgaactcc gggtaaccgc cctccaggtc
     4681 ggagcctttc accgtgacat tgtcgcctgc aagcaggctg atgccgtctc gctccacgcc
     4741 gaagttggcg cggaagccca tgccgccctc agcgaccggc accgagatgt cgtacaggtt
     4801 gggcgagccg gggtgcttca tctccggcgt tccccagcag ggccatggca ggccgtagta
     4861 gtcgccggcg caagggcctt cgcttgcctt cagggtggtg acatcgaagg tgtgcgcatg
     4921 cttcatgtgc agtttgagac gctccggaga ctggccgctg tagccgatac tccaagtgcc
     4981 gcggttgatc tcgcgcaggg tgtcctcgat gctcggttca tcccagccct gccggttttt
     5041 cttcaccttg atgttgacgc aaagttcctg ttcgaagccg aactttttgg cgaaggcata
     5101 catgatgacg tggtcggccc tggattcgaa caaaggctcg atcacctgct cgcgccactg
     5161 gatggagcgg ttggaggcgg tgcaggaacc gtaggtctcg aactgcgtcg ccgccggcag
     5221 cagataggtg ttgtccttgc ggtcctgcat caccgaggcc gccgccgggt agggatcgat
     5281 gacgaccagc agatccagct tttccatcgc tgctttcatc tcggcgccgc gcgtctggct
     5341 gttggcggcc atgccccagt agatcgccgc gcgcaggttc ggcccttggt cgaggtgctc
     5401 gttcttttcc agcacgccgt cgatccagcg cgacaccggc atgccgggtt tttccatcat
     5461 gacttgcgag gcgtagcggc ccttcaccca ctcgtaatcc acgccccata ccgtgcacca
     5521 gtgcttccag gccggttctg tcagcccgta gtagccgggc agcgattccg gattggggcc
     5581 gacgtcggtg gcaccctgca cgttgtcgtg gccgcggaag atgttggtgc cgccgccgga
     5641 cacgccgacg ttaccgagcg ccagttccag aatgcacagg gcgcgggtgt tggcgttgcc
     5701 caccgtgtgc tgggtgatgc ccatgcacca gatcacggtg gcggggcggt tttcgtgcaa
     5761 ggtcttggcg acttgataaa cctcagcctc ggggatgccg gtgacgtcgg ccaccttgtc
     5821 cggcctccag ttggcgactt cctggcggat atcgtccatg ccgtaaacgc ggtcgtggat
     5881 gaattgctgg tcttcccagc cattctggaa aatgtgccac agcacgcccc acacgaacgg
     5941 gatgtcggtg ccggggcgca gccgcacgta ctgatgggca tgggcggcag tacgggtgaa
     6001 gcgcggatcc accacgatca tcctggcgcc tttttccttg gcgtgcagga cgtgcagcat
     6061 tgccaccggg tgtgcctcgg ccgcgttgga gccgacgaac agcatcgctt tggtgttgtg
     6121 gatgtcgtta taggagttgg tcatggcgcc gtagccccag gtgttcgcca cccccgccac
     6181 ggtggtggag tggcagatgc gcgcctggtg gtcgacgttg ttgctgcccc agaacgagac
     6241 aaatttcctc aacaaatacg cctgctcgtt attgtgtttg gaggagccta cccagtacac
     6301 cgcatccggg cctgattcct tgcgaatgga caataatttg gcggaaattt cctccagcgc
     6361 ctgttcccag gaaatgcgct ggtatttgcc gtccaccatt ttcatcgggt acttgagccg
     6421 gcgctcgccg tggccgtgct cgcgcaccga ggcccccttg gcgcagtgcg cgccgagatt
     6481 gatgggcgaa tcgaaggccg gttcctggcg gatccagacg ccattctgca ccaccgcgtt
     6541 gaccgagcag cccaccgagc aatgggtgca caccgtacgc ctggtttcga tcggtccgga
     6601 cgtcctggac tcgctcgttc cggccttggc gatcatgttg aacggcagtt gcgacagcgc
     6661 cactccggcg cccgccgtca ggccggaacg cttgaggaac tcgcgcctgt ccagggtatt
     6721 gcccagccag gaagcgaagc cgctagcagg ggccggcgtg accatcgggg attttttctt
     6781 cagtagcatg cttaaatgcg cgccttgcgg taataggttt gtacgtgttg cgacaggcgg
     6841 tagcccttgc tcgctggcgg cgcttcagcc gtgtcagctt cggtctccgg tgcggtcgcc
     6901 ggcttgccgg tcagcaacgc aacggcggca cccatccccc ctagtcccag gccgagcagg
     6961 aaattcctgc gcttgatgtt ggttttttcc atatccatca ctcctattcc agattcctgt
     7021 gctacccggc catgccgaag gcctgcgctt ctacgtccat gtaggcgcgt agcagcccgg
     7081 cgaccgcccg gtaatagttg gcgctggggg cgagttccag ctgcgtcgca aatttcgtgt
     7141 accacggccg caaattgcgg tcgaaaaacg cacgctgctg tttgatcgaa gccggcgcac
     7201 tatccgcatc gcccgccacc agcagacgca tcacctcgca gatggccgcc atatggtctt
     7261 ccggctcgcc cgccccggct acgcgcgcca gtccgagcgt tgcgagatca tcgcgcaacc
     7321 tggccagcgg ttcttccatc aggaacccgg tcagatgcca ggacaggtag ggcatcacct
     7381 cgccctgtcc caagccgatg aacacctgtt cgtattcctg cgccgctgct tccgcatcca
     7441 tgactgtcga ggccgcctgc aagccctgcc atgcttgtgc cagattcgac ccggcagcca
     7501 gttcatcggc ctgcgcgatg gcccgcaaca gcggcacgtc cggcgcggtg tacagcaggc
     7561 gcgcgagcaa ggcatacaat ccggcgcgcg cctggtcttc ctcagtcaac ggcaagtctc
     7621 cggccacggc attcatttga ccgccatcgt ttccccggct tccatcgtgt ccttcacccg
     7681 gcaatcgccg cacatttgca aacggcgcct cgcttccggc gtggcaaaca tggaatgccc
     7741 ggccagcttg tgcagtatgg cgccgaccat gtgcgtggtg gcgaacggtt tgccgcagcg
     7801 cacgcaggga aacggccggt cttcatgcag cacgcgcgcc tgcttggcgg ctggggtcag
     7861 caacaatcgc ggctgcaggt ggatggcatc ctcggggcag gcgctttcgc acaagccgca
     7921 ctgcacgcag ttgcgctcga tgaatttcag ttgcggcgcg ccggcgccat cctgcagcgc
     7981 ggcggccggg cagacgctga cgcaggacag gcacagggtg caagctgcgc cattgatctc
     8041 gaccgcgccg aatggggcgc cggtcggcaa ggcaagcgcc tctggtacgc tggtgggagc
     8101 gtggttggca aaatgttcga tgcagaattc cagggtggtg cgcttgtcgt cgaacaggcg
     8161 gaacgcagcc cgctgctgcg gcacgctgcc tagcgcttcc tctgccaatg cctgctgcaa
     8221 agccgctgca tccgctgttt cgatcaggct gaaatgaccc tcgccatagc ccaattcttg
     8281 cagaattttc gaccccagcg ccatctgtgt gtgcagtgcc gtcaggtatt gcggcgcctc
     8341 gctgccggcg gcgagaatga cgacgcgacc ggcgcccagc gccagtgcgc ccagcagcca
     8401 gtccaggccg atgctggcga catggaaaat ttccagcggg atgacatttg ccggtaaggc
     8461 gctggcctgt actagtgcgg cgccgtcacc gggattgtgc agtaacaggc aggcatccgt
     8521 gccgcccgcc gttcggtagg ctgccagtgc cgcctgcagc ttgccgcccc agtaggacac
     8581 cgaggggaag ttgtactgca tggcaccgct gggacacacc gtggcgcagg cgccgcagcc
     8641 catgcacagg tgcggctcga cgcggatatg ctcgccctgg gcactgatcg cttcggtgga
     8701 gcatacctcg atgcagcggt cgcagcccgg caggcgcgag cggctgtggg cgcaggtttt
     8761 ttcctgataa aggaagaatt tcggcttctc gaactcacct accgcgtcaa ggatttccgc
     8821 cgtggcctgc tgcagcgtgg ccacgtctgc cggcgcgaaa tagccttgtg gcggctggtg
     8881 catggtgaag tagcggttgg cggtgaggtt cagtaccagg tcgaaattgc cgctgccgcc
     8941 agtccacgtt gccgtgaact cgcccagcca gcccgtgatg atttcgatct cgccgtagaa
     9001 gattggcagg ctggtgcccg tagttgcacc tgcgggcttg gttagcagca ggctcaccgt
     9061 tagcttgtcg gccagatcgc gcgccaccgc caatgcttgc ggcgtatcgc cgatcaccag
     9121 cacgtgtccg gccgagcggt attcgaccac cggcaccggc tttggagcag gataggccgc
     9181 gatggcggcc agcacggatt gtacgggcgg actcatacca actccggttt ttcctgggtt
     9241 gggtcggaag tcttttcttc tgctggcgcc agcatggcct gtgcgtgatc cagcgtggct
     9301 aacagttccg ccggcagttc ggccggatgg gaatagtcct cgacgtatac gtccagtccg
     9361 tccatggtcc gatagtggtc ggtcatgaac agcttgcgca gggcggtgcg gcgcgttgca
     9421 ccgtccacct cggggtgcat gaacgggctg aagtcggcgc ggaagccgag ttcagtcatc
     9481 gccggcagtg ccggggtagt ggtcgggacc ggtgtggcgg gttcagcggc agtctggagt
     9541 ttgaggcgcg accagcggcg gatgaaggaa aggggcttgc tcatgggggc gtaattttaa
     9601 cacggcgcgc gcccgactag ccagcaaaaa tccagcaaaa tcaatgtcca taggggtttc
     9661 aggactttgc catggtcagg atttcgccat ggcggaaaca gtccacactg tggtcgttga
     9721 ccatgcccac cgcctgcatc atggcgtagc agatggtgga gccgatgaac ttgaagccgc
     9781 gccgggcgag gtccttggac agggcatcgg aaatcggcgt ggtagccggc acctcttgcc
     9841 atgaagccca ggcattctgg atggggttgc cgtccacgaa gcgccacagg taggcatcga
     9901 agctgccaaa ttcttgttga acttgcagcg tgcgttgcgc gttgttgacc gccgccgcga
     9961 ttttcagccg gttgcgcacg atacctgggt cggccagcag ctgcgctact tttgcatcat
    10021 cgtagcgcgc cacccgttgc gggtcgaagt cgtcgaatac ctcacggtac ctggcgcgct
    10081 tgttgaggat ggtcgaccac gacagccccg cctgtgcacc ttctagcacc aggaattcga
    10141 acagtgcgcg gtcgtcatgc tgcggcacgc cccattcggt gtcgtggtag gcgatgtcga
    10201 gcggggattt ggcccagacg cagcgataca tctagtgaaa cagcgccaac gccttcttga
    10261 aagtctggaa cacgccccag cccagcggga tcaggacgta ggcccagaac agcacgagca
    10321 ggacggcgtg gttttcggtt ttctggcttt cggtgttttc catttctgtc tcctcagagg
    10381 tttccggctg cgccgagggc aaggtcaccc ctgcaacgga tcatccactt cgtcgtgctt
    10441 catgtagtgc ttctcatgca cttccttcat cagcaggttg cagacgaagc cgacggtgag
    10501 cagtccggcc atgatgtaga gcgtggtggt gtaggcatca gccttggcca cgccctggct
    10561 gatctggtat tcgcggatgt agttgaccag caccgggccg agtacgccgg cggtggacca
    10621 ggcggtgagc agccggccgt ggatgccgcc gacgtacttg gtaccgaaga tgtccgccag
    10681 ataggccggg atggtggcga agccgccgcc gtacatgctg aggatgacgg cgctggcggc
    10741 gatgaacagc acgatgttgc cctcgccgct gaaggtgggc agcatggaat agaggaaagt
    10801 gccgagggcg aagaacagga agtaggtgtt cttgcggcct aaataatccg acgccgacga
    10861 ccacacgaag cgcccgccca tgttgaacag cgaaagcagg ccgacaaagc cggcggcagc
    10921 ttccggcgtg atgttgccct tgaacatttc ctggatcatg accgatgcct ggccgaggat
    10981 gccgatgccg gcggtgacgt tgaggcacag gatcagccat agcaggtaga actgcggggt
    11041 tctgagcgcc tggtcgatat gcacgtggcg cttggtgacc atcttcttgg cctccgccgg
    11101 tggcgtccag cccttcggtt tccagtcggc cggcggaatg cggatggcga gcgagccgat
    11161 gaccatagaa atgaagtaga ccacgcccat gaccacgaag gcttgcatga cgccgaccga
    11221 agtgtcgctg gcgaagtgct tcatcagcgc cacgcccagc ggcgcgccta tcatggcgcc
    11281 gccgccaaag cccatgatgg ccatgccggt ggccatgccg cgccggtccg ggaaccattt
    11341 gatcagggtg gaaaccggcg acacgtagcc cagtcccaga ccgcagccgc cgagcacgcc
    11401 gtagcctaga taaagcagcc agatctggtg ggtcttgatg ccgatggcgg aaatcagaaa
    11461 gccgccaccg aagcagcagg cggcagtgaa catggtgcgg cgcgggccga ccttttccag
    11521 ccacttgccg ccgaaggcag cggaaaggcc gaggaagacg atggcgaggc tgtagatcca
    11581 gccgatggtg gtgagtttcc agtcgtccgg ggcggattcg gcgacgccga tgacgcgcgt
    11641 cagaggttcg ttgaagacgc tgaaggcata ggcctgaccg atgcacaggt ggacggccag
    11701 cgccgccggc gggatcagcc agcggttata gttgtggccc gcggtaattc tttccctgga
    11761 aaaaaagccc gacatacacc tctccccgaa acttattgtt ggaaactaaa gcttgctggt
    11821 actcctgttg acaattgaaa caacataggc ggatggtctt acaaatcctg gcaaatggca
    11881 acgggggtag cgatttgtca cgcaggcttg atttaagcat tcgttgtcat caatatgtca
    11941 cataatcttc atatagtgca cagcattctg accggaaaaa ggaagccaga tgccagccaa
    12001 tatcctcgtc gtcgaagacg agcccgccat ccaggaactg cttgcgctca atctcacaca
    12061 ggcaggccac aatgccattc gcgccttgag cgtggagcag gcgcaattgc tgatgcgcga
    12121 aacaatgccc gacctcatca ttctggactg gatgttgccc ggcaccagcg gtatcgattt
    12181 tgcccgccgc ctcaagtcgg acgagtacag caggggcatc cctatcatca tgctcaccgc
    12241 gcgtggcgag gaagccgaca aggtgcgtgg gctggaggtg ggtgccgacg attacgtcac
    12301 caagccgttc tcgccgcgcg agctcaacgc ccgcatcaaa gctgtgttgc gccgccgt
//
LOCUS       DAOMFA010000033        12168 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_504_length_12168_cov_5.622307, whole genome shotgun sequence.
ACCESSION   DAOMFA010000033 DAOMFA010000000
VERSION     DAOMFA010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 12168)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12168)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12168
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_504_length_12168_cov_5.622307"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1015)
                     /locus_tag="PKW44_02950"
     CDS             complement(<1..1015)
                     /locus_tag="PKW44_02950"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HQR50581.1"
                     /translation="MHWIAPSERDAASTSLEKRLWDAADQFRANSGLKAQEYSGPILG
                     LIFLRFAEVRFAIQRSKLSLPSPSGRGVGGEGAQPSRRGSRVDEPAAYHAEGILYLAP
                     EARFDYLLTLPEVADIGARVNAAMRLIEQHNPQLAGVLPKTYNLFTSSLLKELLKKVS
                     EIPATLDYDAFGRIYEYFLGAFAMTEGQGGGEFYTPSSIVKLLAEIIEPFHGRILDPA
                     CGSGGMFVQSARFVAEHQKNPAAELAICGVEKTDETGRLCRLNLAVHGLEGDIKHGGN
                     INSYYDDPHNATGSFDFVLANPPFNVNAVDKERLKDMVGAGRRFPFGLPRTDNANYLW
                     IQLF"
     gene            complement(1030..1917)
                     /gene="cysM"
                     /locus_tag="PKW44_02955"
     CDS             complement(1030..1917)
                     /gene="cysM"
                     /locus_tag="PKW44_02955"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase CysM"
                     /protein_id="HQR50582.1"
                     /translation="MFRSIEDFIGGTPLVRLQRLPGATSNTILVKLEGNNPAGSVKDR
                     PALSMIQRAEARGDIKPGDTLIEATSGNTGIALAMAAAIKGYRMVLVMPENQSVERRQ
                     TMKAYGAELVLTPKAGSMELARDTAERMQSEGKGRILDQFANADNPQAHYEGTGPEIW
                     RDTAGKITHFVSSMGTTGTIMGTSRYLKEMNPAIRIVGVQPQEGAQIPGIRKWPQEYL
                     PKICDFSRIDHMIYVGQKDAEETTRRLAREEGIFAGISSGGALFAALQLSKEVENAVI
                     VSIVCDRGDRYLSTGVFPE"
     gene            complement(1920..2420)
                     /locus_tag="PKW44_02960"
     CDS             complement(1920..2420)
                     /locus_tag="PKW44_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HQR50583.1"
                     /translation="MLGKLLHGRTPYFLGAAASFGLVGLAIFLQLRNNLDPCPLCISQ
                     RIAFMALGTAFLLAALHNPAGLWRKLHGLLQLAAAATGAAIAARHIWVQAHPGQTMSE
                     CGVGFDYMIHQFPMSKAVRLIFQGTGECSVIDWTLFGITIPQLSLIAFTGLGVYAIVL
                     AFAKGR"
     gene            complement(2539..3162)
                     /locus_tag="PKW44_02965"
     CDS             complement(2539..3162)
                     /locus_tag="PKW44_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR50584.1"
                     /translation="MGVLLLCWGALAMAADTVMEAVVELQHGWANAYYQVPEKQKESA
                     FKELAAKAHTVSAANPDRVEPMVWEAIILSSYAKFAGGLGALSKAKASRDLLEKAEKI
                     QPDALNGSIYTSLGSLYYKVPGWPIGFGDKKKAREYLDKALKLNPDGIDPNYFYGDFL
                     LEQGEYARAQEYLQKALAAPARPGREDADQGRRAEVQQALEKAKQHG"
     gene            complement(3206..4009)
                     /locus_tag="PKW44_02970"
     CDS             complement(3206..4009)
                     /locus_tag="PKW44_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018506647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQR50585.1"
                     /translation="MNLSGRRILITGAAGGIGSELSFALAEKGAQLLLADRDEQKLER
                     LKTDIQVRGGQAQVIPCDLAAADGPHMLAAQAIAAIGGVDVLVNCAGIASFGVFAEQA
                     PESLETLWRVNVLAPMKLTQALLPQMASRGHGRIVNLGSVFGSIGFAWFAAYSASKFA
                     MRGFSEALRRELEGTGVGVTYVAPRYTRTSLNDGAVSRMAQAVGMNSDEPSVVAQHVV
                     RAIERDCKDYYIGWPECLFVRINAILPRLVDGALRKQNARTREFAENQA"
     gene            complement(4006..4659)
                     /locus_tag="PKW44_02975"
     CDS             complement(4006..4659)
                     /locus_tag="PKW44_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019916873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HQR50586.1"
                     /translation="MTFFEQLQQATQAEQAELQSAPIIRKALAGQVTLNQYVAFLTQA
                     YHHVKHTTPLLMACGARLGDDKEWLREAIAHYIEEEIGHQEWILNDIKACGADAEAVR
                     HGQPAFATELMVAYAYDTIARGNPIGFFGMVHVLEGTSIQLAINAADTLQQSLGLPKQ
                     AFTYLNSHGSLDLEHVDFFRDLVNQLESEQDKACLVHCAKAVFRLYGDIFRSLETVQ"
     gene            complement(4646..6142)
                     /locus_tag="PKW44_02980"
     CDS             complement(4646..6142)
                     /locus_tag="PKW44_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HQR50587.1"
                     /translation="MSILLQTLHQIALISPERVALQGAHQAMTYGELDKSVAESAHEL
                     NAQDFRVLGLLADNGIPWALADLSALAASIPLVPLPLFFSPQQIVHVVHDAGLDGLLT
                     DQPDQVEALLKGANLRCHIAGELCGLSLIRLQGVAPRPLPAGTAKITYTSGTTGEPKG
                     VCLSLTQMEAVAASLTGASEAQAEYRHLCLTPLSTLLENIGGIYTPLLAIASTCLLPL
                     SQVGLRGASGLDVARMLQALQECAASSAILAPQMLHALVAAGESGAPMPASLQFVAVG
                     GAPVSPRLLQRAQQLGLPVFEGYGLSECASVVALNTPHANRPGSVGRPLPHVTLSFSE
                     QSEILVSGSTFLGYLGQGKSPEPCPTGDLGYLDAEGFLHLTGRKKSVFITSFGRNVAP
                     EWVERELTLHPAIAQAAVFGEGKPFNVAVIVARPGFSAADIAAAVQLANRMLPDYARV
                     SNWVAASAPFTPSNQQLTANGRLKREAIRKCYADAIEFLYQEETHDVF"
     gene            complement(6139..6840)
                     /locus_tag="PKW44_02985"
     CDS             complement(6139..6840)
                     /locus_tag="PKW44_02985"
                     /inference="COORDINATES: protein motif:HMM:NF023682.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermostable hemolysin"
                     /protein_id="HQR50588.1"
                     /translation="MSATPASLQSEFLDCSANDAHVEALNVRLAQVFDLREHENEERG
                     EIESFIRQCFAAAHGARINQFMPRLLSLRAKRGDLIAAFGLRSAADSRLFLETYLDRP
                     IEAVLQARLGQAVNREEVIEVGNLSVLYPGAARWLIVALTAMLHEEGYKWVVFTGTAS
                     LRNGFSRLGLRPVELGAATLEHLSPSDRADWGHYYDHTPLVMAGDIAYGYRSLLMQRD
                     LAELLRTGMDSVETA"
     gene            complement(7045..7506)
                     /locus_tag="PKW44_02990"
     CDS             complement(7045..7506)
                     /locus_tag="PKW44_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR50589.1"
                     /translation="MRCLLLLCLLLLPAFSQADEAIAVIVPPGHGKALKKDDLALVFK
                     RKKLFWSDGGKMQPVNLPASNPLRRTFSLTVLGATPEELEQYWNDRYFHGVSPPFVAG
                     SEEAVLRFVAETPGAIGYVPLCSVDDRVTVALVISAAGHISEDAASASCPR"
     gene            complement(7524..8546)
                     /locus_tag="PKW44_02995"
     CDS             complement(7524..8546)
                     /locus_tag="PKW44_02995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR50590.1"
                     /translation="MLLVIQTAAWAEDLEQASSYELGKGWQVPGTSFNLGGYASGGLS
                     DDHGEGWVLGMNDLSLFVRWEGEGKLRLFSELDLEEPVGIEEGVGLTTKHAYLGLERL
                     YLDYLYSEKLNFRGGKFLTPIGRWNLLHAAPLVWTTTRPLITEYTFPTNATGAMAYGT
                     LPLFGTEVDYSVYSAIGNDWRPDPKLDPFEEAHGLHFNVPLGRAGQLGMSYANFEQKS
                     SSGEHQNLVGLDYYWEHNRLEISSEAVYRSSDDGGSEDEKGLFVQGAIPLTERLHGIV
                     RYEVFDQAGNAPTINLWLAGLALRVAPSVLLKAEYSHANHGTVEFSHGMNNVPDGLFT
                     SFAIMF"
     gene            8657..10420
                     /locus_tag="PKW44_03000"
     CDS             8657..10420
                     /locus_tag="PKW44_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018506641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQR50591.1"
                     /translation="MTIRKTLLVAFLLVSLLPSILLTYLAFHVAGDAMRSDIEHNLQV
                     QAATVSQDIDKMLFERLQNAQTWHRLEVMQDIHINDVDKRLSKFLAELKVGYRDVYLE
                     LFCTNTEGRVVASSDPASIGSIRPQGEGILLTGDTNVPIRLGTLQLTGTDGSAVLPIS
                     ANVPSMFNDGQIGELQLLFNWAQIYRILDQSAQSGHELALLDPDRRIIAASSKLRERN
                     LLQQQVPANWIASGRTGIGTYDGRLLGMNEVMVGYDRSPGFQQFPGFSWTTLVIESHH
                     QAFIPVRQMALVFLLLLALTSIVAIAFSLLVAGRIARPIAALTEFTRRFKRDQQLPEA
                     PQAIGGEVGELTEAFVQTVRDLDQSRTDLVRASKLAVLGEMAAVMAHEIRTPIGILRS
                     SAQMLAREPELRPEAQELTGFIESETERLNRLVSTLLDSARPRAPKLQPADLHAIIRH
                     SIDLLAAQADKKNIHIHLKLYGRHPVIELDAEQMTQVLLNLVLNALQILPQGGQVEIT
                     TREDAGKLVLEIADDGPGIPPQELVRVFDPFFTKREGGVGLGLAVVQQIVAAHGGEIH
                     AGNSALGGALFTITLPMKESS"
     gene            10417..11766
                     /locus_tag="PKW44_03005"
     CDS             10417..11766
                     /locus_tag="PKW44_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020183101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HQR50592.1"
                     /translation="MTGQHVLVVDDEAKMQRVLEIMLKRMGHAVASASNGREALQILK
                     SSPVDLIISDLRMPEMSGTELLQALRQQGNEVPVIIMTAYGTIESAVEAMKLGACDYI
                     VRPFDVEALEIAINRILAAGRMRRQNDFLRHEVDKGWGEFIGNSPPMQTIYDLIRQVA
                     PGKTAVLITGETGTGKELVARAIHRASPRHEALFVPINCAAIPGDILESELFGYEKGA
                     FTGASKDRIGKFEMADGGTLFLDELTEMPMALQAKLLRVLQENVIERLGSNRVIPIDV
                     RIVTATNRDPRQAIAEGKLREDLYYRVNVFNIDLPPLRERKQDIPALVAGFLSGHAGT
                     HGISEAALARLQDYAWPGNVRELQNVIERAAVLSRGSVIQMEHLPREMTTADAESVIA
                     ESPQFHSLELNPAVENLERTMIRAALLQTGGNKSKAARLLDISERSLWYKIKNYGLE"
     gene            11772..>12168
                     /locus_tag="PKW44_03010"
     CDS             11772..>12168
                     /locus_tag="PKW44_03010"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019899308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HQR50593.1"
                     /translation="MPAEPLFTKPAVLSVSDLNRLAREVLEHSFPLFWVAGEISNLTR
                     AASGHWYFSLKDDKAQVRCVMFRNKNMLLDWQPKEGDHTEARALVTLYEARGDFQLNI
                     EALQRAGLGALFEAFERLKARLEYEGLFDA"
BASE COUNT         2474 a         3732 c         3691 g         2271 t
ORIGIN      
        1 agaagagctg gatccacagg tagttggcgt tgtcggtgcg cggcaggccg aaggggaagc
       61 ggcgaccggc gccgaccatg tccttgagcc gttccttgtc caccgcattg acgttgaacg
      121 gcgggttggc aaggacgaaa tcgaatgatc cggtggcgtt gtgcgggtcg tcgtagtagc
      181 tgttgatgtt gccgccgtgt ttgatgtcac cttccagccc atgcacggca aggttgaggc
      241 ggcacaggcg gccggtttca tcggtctttt cgaccccaca aatggcaagc tcggcggcgg
      301 ggttcttctg gtgctcggcg acgaagcgcg ccgactgcac gaacatgccg ccggagccgc
      361 aggcggggtc gaggatgcgg ccgtggaagg gttcgatgat ttcggccagc agcttgacga
      421 tgctgctcgg cgtgtagaac tcgccgccac cctggccctc agtcatggcg aaggcgccga
      481 ggaagtattc gtagatgcgg ccgaaggcgt cgtagtcgag cgtggccgga atctcggaga
      541 cttttttcag cagttccttg agcagggagc tggtgaaaag gttgtaggtc ttgggcagca
      601 cgccggcaag ctgcgggttg tgctgttcga tgagccgcat cgcggcgttg accctggcgc
      661 cgatgtcggc cacctcgggc agcgtcagca aatagtcaaa acgcgcctcg ggggcgaggt
      721 agaggatgcc ttcggcgtgg taggcggcgg gttcatcgac gcggctgcca cggcgggatg
      781 gctgcgcgcc ctcacccccg acccctctcc cggagggcga ggggagcgaa agtttgctgc
      841 gctggatggc gaagcggact tcggcaaagc gcaaaaagat caggccaagg atgggtccgg
      901 agtattcctg cgccttgagg ccggaattgg cgcggaactg gtcggcggca tcccacaggc
      961 gcttttcgag cgaggtggag gcggcgtctc tttcggaggg ggcgatccag tgcataaacg
     1021 actttcttgt tattcgggaa aaactcccgt cgacaggtag cggtcgccgc ggtcgcagac
     1081 gatggagaca atcactgcat tttccacttc cttcgacaac tgcaatgcgg cgaacagcgc
     1141 gccgccggag gagatgccgg cgaagatgcc ttcctcgcgc gcgaggcggc gggtggtttc
     1201 ctcggcatct ttctggccga cgtagatcat gtggtcgatg cgcgagaagt cgcagatttt
     1261 cggcaggtat tcctgcggcc acttgcggat gccggggatt tgagcgcctt cctgcggctg
     1321 cacgccgacg atccggatgg cggggttcat ttccttgagg tagcgcgagg tgcccatgat
     1381 ggtgccggtg gtgcccatgc tggagacgaa gtgggtgatc ttgcccgccg tgtcgcgcca
     1441 gatttccggc ccggtgccct cgtagtgcgc ctgcgggttg tcggcgttgg cgaactggtc
     1501 gaggatgcgg cccttgccct cggactgcat cctttccgcc gtatcgcgcg ccagctccat
     1561 gctgcccgcc ttcggggtca gcaccagctc ggcgccgtag gccttcatgg tctggcggcg
     1621 ttccaccgac tggttttccg gcatcaccag caccatccgg tagcccttga tcgctgccgc
     1681 catcgccagc gcgatgccgg tgttgcccga ggtcgcctcg atcagcgtgt cgccgggctt
     1741 gatgtcgccg cgtgcctctg cgcgctggat catggaaaga gctggtctat ccttgaccga
     1801 accagccggg ttgttgcctt ccagtttgac caggatggtg ttggatgtgg cacccggcag
     1861 gcgctgcagc ctgaccagcg gcgtgccgcc gatgaagtcc tcgatgctcc tgaacatgct
     1921 tacctgcctt tggcgaacgc cagcacgatg gcgtagaccc ccaggccggt gaacgcgatc
     1981 agcgacagct gcgggatggt gatgccaaac agcgtccagt cgatgaccga gcattcgccg
     2041 gtcccctgga agatgagccg gaccgccttg ctcatgggga actgatggat catgtagtcg
     2101 aaccccacgc cgcattccga catcgtctgg cccggatggg cctgcaccca gatgtggcgg
     2161 gcagcgatcg cggcgccggt cgctgcggcg gccagctgca acaacccgtg caacttgcgc
     2221 cagaggcctg ccgggttgtg cagcgcagcc agcagaaagg ctgtgcccag cgccatgaag
     2281 gcgatgcgct gcgagatgca cagcgggcaa ggatccaggt tgttgcgcaa ctggaggaag
     2341 atcgccagtc cgaccaggcc gaagctggcg gcggcgccaa ggaagtaagg ggtacggccg
     2401 tgtagaagtt tgcctaacat accaagagtc cgaacgacgt tgaggcgctc agtataatgg
     2461 acagcgcgag cttgcagagc ataaaaaacg gcggccgcag ccgccgtaac ccgctcgtcg
     2521 gtgaaacagg ggctgccctc aaccgtgctg tttggccttt tccagcgcct gctgcacttc
     2581 cgcgcgccga ccctggtcgg catcctcacg gccggggcgc gccggtgctg ccagcgcctt
     2641 ctgcaggtat tcctgggccc tggcgtattc gccctgctcc agcaggaaat caccatagaa
     2701 ataattgggg tcgatgccat ccggattgag cttgagcgcc ttgtccagat actcgcgcgc
     2761 ctttttcttg tcgccgaagc cgatcggcca gcccggcacc ttgtagtaga ggctgccgag
     2821 cgaagtgtag atggagccgt tgagcgcgtc cggctgaatt ttctcggctt tctccagcag
     2881 gtcacgggac gccttggcct tgctcagggc gcccaaacca ccggcgaact tggcatagct
     2941 gctgaggata atggcttccc agaccatggg ctcgacgcgg tcgggattgg cagcactgac
     3001 ggtatgcgcc ttggcggcga gttccttgaa cgccgattcc ttctgctttt ccggcacctg
     3061 gtagtaggca ttggcccagc cgtgctgcaa ttcgaccacg gcctccatca ccgtatcggc
     3121 agccatggca agcgcgcccc agcacaacag caggacgccc ataaaaaacg cgttaaattg
     3181 caatttcatc tttactctcc ttcaattaag cctggttttc ggcaaactcg cgggtcctgg
     3241 cgttctgctt gcgcagtgcg ccgtcgacca gtctcggcag gatggcgttg atgcgcacga
     3301 acaggcactc cggccagccg atgtaatagt ccttgcagtc gcgctcgatg gcgcgcacca
     3361 catgctgcgc caccaccgaa ggctcgtcgc tgttcatacc aaccgcctgc gccatgcggc
     3421 tcactgcgcc atcgttgagc gaggtcctgg tgtagcgcgg ggcaacgtag gtgacgccga
     3481 cgcccgtccc ttccagctcg cgccgcaacg cctcggaaaa accgcgcatg gcgaacttgc
     3541 tggcggagta agcagcgaac caggcaaagc cgatgctgcc gaataccgaa ccaagattga
     3601 cgatgcggcc atggccgcgc gacgccatct gcggcaacag cgcctgcgtc agtttcatcg
     3661 gcgccagcac gttgactcgc cacagagttt ccagactctc cggcgcttgt tcggcgaaca
     3721 cgccaaagct ggcgataccg gcacagttca ccagtacgtc cacgccacct atcgcggcaa
     3781 ttgcctgcgc ggccagcata tgcggcccat ctgcagccgc cagatcacac gggatgacct
     3841 gcgcctggcc gccgcgcacc tggatatccg tcttcagccg ttccagcttc tgctcgtcgc
     3901 gatcggccag cagcagttgg gcgccctttt ccgccagggc aaacgagagt tcactgccga
     3961 taccgccggc agcaccggta atcagaatac gtcgaccgga gaggttcatt gcacggtctc
     4021 caggctgcgg aaaatatccc cgtacagccg gaacacagcc ttggcgcaat gcaccagaca
     4081 ggctttgtcc tgctccgact ccagttggtt gaccaggtcg cggaagaaat ccacgtgttc
     4141 cagatcgagc gagccgtggc tgttgaggta ggtaaaggcc tgcttcggca agcccagtga
     4201 ctgctgcaag gtgtcggcgg cattgatcgc cagttgaata ctcgtccctt ccagcacatg
     4261 caccatgcca aagaagccaa ttgggttgcc gcgcgcaatc gtatcgtagg cataggccac
     4321 catcagctcg gtggcgaaag ccggctgacc gtggcgcacc gcttcggcat cggcgccaca
     4381 ggccttgatg tcgttgagta tccattcctg gtggccgatt tcctcctcga tgtaatgggc
     4441 gatggcctcg cgcagccatt ccttgtcgtc gccaagacgg gcgccgcagg ccatcagcaa
     4501 gggggtggtg tgcttgacat ggtggtaggc ctgggtgaga aaggcgacat actggttcag
     4561 cgtcacctgt ccggccagcg ccttgcggat gatgggggcg gattgcagct cggcctgctc
     4621 ggcctgcgtg gcctgttgca gttgttcaaa aaacgtcatg cgtttcctcc tgataaagaa
     4681 attcgatcgc gtcggcatag catttccgga tagcctcgcg cttcagccgg ccgttggccg
     4741 tcagttgctg gttggatggg gtaaacgggg cgctcgctgc cacccagttg gagacccgtg
     4801 cataatccgg cagcatgcgg ttggccagtt gcacggcggc ggcgatgtcc gccgctgaaa
     4861 aaccggggcg cgccacgatc accgcgacat tgaacggctt gccttcgccg aacaccgccg
     4921 cctgggcgat ggcggggtgt aacgtgagtt cgcgctccac ccattccggc gccacgttgc
     4981 ggccgaacga agtgatgaac acgctcttct tgcgcccggt caggtgcagg aatccttcgg
     5041 catccagata gccgagatcg cccgtcgggc atggctctgg cgacttgccc tggccgagat
     5101 agccgagaaa agtcgaacca ctcaccagaa tttcgctctg ctcggaaaaa ctaagcgtga
     5161 catgcggcaa cgggcggccg acgctgccgg gacggttggc gtgtggcgtg ttgagcgcca
     5221 ctaccgaagc gcactccgac aggccgtagc cttcgaatac gggaaggccc aattgctgcg
     5281 cgcgctgcaa caagcgcggc gataccggcg caccgccgac ggcaacaaac tgcagactgg
     5341 cgggcatggg tgcgccgctc tcgcccgccg cgaccagtgc atgcagcatt tgcggcgcca
     5401 gaatggcgct gctggccgca cattcctgca gtgcctgcag catgcgcgcg acatccaacc
     5461 cggaggcgcc gcgcaggcca acctgcgaca acggcagcag acaagtactg gcaatggcca
     5521 gcagcggcgt atagatgccg ccgatgtttt ccagcaaggt ggaaagcggc gtcaggcaca
     5581 ggtgacgata ttcggcctgc gcctcgctgg cgcccgtgag tgacgcggca acggcttcca
     5641 tctgcgtcag actgagacaa acgcccttgg gctcgccggt agtgccagag gtataagtaa
     5701 tcttggccgt gcctgccggg agcggcctgg gcgccacccc ttgcaaccgg atcagggaca
     5761 ggccgcacaa ctcgccggcg atgtggcagc gcaggtttgc gcctttcagc aaggcctcga
     5821 cctggtcagg ctggtcggtc aacaagccat ccagtccggc atcgtgcacg acgtgcacaa
     5881 tttgctgagg ggagaaaaac agcggtagtg gcaccagtgg aatgctggcc gccagcgccg
     5941 acaggtcagc cagcgcccac ggaatgccat tgtccgccag caaccccagg acacggaaat
     6001 cctgggcgtt caattcatgc gcagactcag cgacagactt atccagttcg ccataggtca
     6061 tggcctgatg cgcaccctgc agcgcgactc gttccgggct gatcaaagcg atctggtgca
     6121 gcgtttgcag caggatgctc atgcggtttc gacgctatcc atgccggtgc gaagcaattc
     6181 ggcgagatcg cgctgcatca gcagcgagcg gtagccatag gcaatgtcgc cggccatgac
     6241 cagcggcgta tggtcgtaat aatggcccca atcggcacga tccgacggcg acaggtgctc
     6301 cagcgtcgcc gcgcccagct cgaccggacg caagcccaga cgactgaagc cgttacgcag
     6361 cgaagcggtg ccggtaaaaa ccacccactt gtagccttcc tcgtgcagca tggccgtcag
     6421 cgctacgatc agccagcgcg cggcgccggg atacaataca gagaggttgc cgacctcgat
     6481 gacctcctcg cgattcaccg cttgccccaa acgggcttgc agcacggcct cgatcggacg
     6541 gtcgaggtag gtttccagaa acaggcgaga atccgcggca gaacgtaggc caaaggccgc
     6601 gatcaggtca ccgcgcttgg cacgcaggct gagcaggcgc ggcatgaact ggttgatacg
     6661 cgcgccgtgg gcggcagcga agcactgccg aatgaaactt tcgatttcgc cgcgctcttc
     6721 gttttcgtgt tcgcgtaaat caaacacctg cgccagacgt acattcagcg cctcgacatg
     6781 cgcgtcgttg gccgaacaat ccagaaattc gctttgtaac gaggctggtg ttgcagacat
     6841 gtaatttcct ctcggcatat ttaagctaag ggggtaactg caatccctgt gccaaaggct
     6901 taaaaaaccc ggaaattatt aattatgcta ttgtttttat ttaaaaaata ttattttaat
     6961 tgaattttca tgggaaaaga tatctgtaga aaaaccccgc acaaaaggga aaacgcgcaa
     7021 gtttattcta caatttttgc agggttatct ggggcaagaa gccgaggcgg catcttcact
     7081 gatatgcccg gcagcgctga tgacaagcgc aacggtcacc ctgtcatcca cgctgcaaag
     7141 tggcacatag ccgattgcgc ctggcgtctc cgcgacaaag cgcagcaccg cttcttccga
     7201 gccagccacg aagggcggcg agacgccgtg gaagtacctg tcgttccagt actgctcgag
     7261 ctcttccggc gtggcgccaa gcacagtcag ggaaaaagtc ctgcgcagcg ggttggaagc
     7321 cggcaggttg accggctgca tcttgccgcc gtcgctccag aacagtttct tgcgtttgaa
     7381 gaccagggca aggtcatcct ttttcaacgc cttgccatgg cctggcggta cgatgactgc
     7441 gatcgcctca tccgcttgcg agaaggcagg cagcagcaac aggcataaca gcagcaagca
     7501 gcgcatgaaa aatccagggc gcatcagaac atgatcgcga aagaagtaaa caacccgtca
     7561 ggcacgttgt tcataccatg gctgaactcg acagtgccat ggttagcatg gctgtattcc
     7621 gccttgagca gcaccgaggg tgccacgcgt aacgccagac cggctagcca gaggttgatg
     7681 gtgggtgcat ttcctgcctg atcaaacacc tcatagcgca cgatgccatg caggcgttct
     7741 gtcagcggaa tggcgccctg aacgaacagg cctttttcgt cctcactgcc accgtcgtca
     7801 gatgagcggt ataccgcttc ggagctgatt tcgagacggt tgtgctccca gtagtaatcc
     7861 agaccgacca gattctggtg ctcgccactg gaacttttct gctcgaaatt ggcgtaggac
     7921 atgcccaatt gcccggcacg ccccagcgga acattgaaat gcaggccatg tgcctcctcg
     7981 aaaggatcga gcttggggtc gggacgccag tcattgccga tggcgctata caccgaataa
     8041 tcaacctccg taccaaacag cggcaaagtg ccatacgcca tggcaccggt agcgttggtg
     8101 gggaaggtat attcggtaat cagcgggcgg gtggtagtcc acaccagcgg tgccgcgtgt
     8161 agcaggttcc agcggccgat gggggtgagg aacttgccgc cacggaaatt gagtttttcc
     8221 gaatagagat aatccaggta aagccgttcc agcccgagat aggcatgctt ggtggtcagt
     8281 ccgacccctt cttcaatccc gaccggctct tccaggtcaa gttcggaaaa caggcgcagc
     8341 ttgccctcgc cttcccagcg cacgaacagg ctgaggtcgt tcatgcccag cacccatccc
     8401 tcgccatggt cgtcgctgag tccgccgctg gcgtagccgc ccaggttgaa gctggtaccg
     8461 ggcacctgcc agcccttgcc caactcgtaa ctggaggcct gttccaggtc ctcggcccaa
     8521 gccgccgtct gtattaccag caacaatagc gtcagcggaa ttcgaacgca cttgatcggg
     8581 aacatatcgc cccacaggat ggaaaaaaac ggattgtgcc atatacttgg gcctccacat
     8641 ccagtccatt ttcaccttga ccatacgcaa aacattgctg gtcgctttcc tgctggtcag
     8701 cctgctaccc tcgatactgc tgacctatct ggccttccac gtcgccggcg atgccatgcg
     8761 cagcgacatc gagcacaacc tgcaggtgca ggcggcaacg gtttcacagg atatcgacaa
     8821 gatgctgttc gagcgcttgc aaaatgcgca aacctggcac cggctggagg tgatgcagga
     8881 catccacatc aacgacgtgg acaagcgcct gtccaaattc ctggccgaac tcaaggtagg
     8941 ctaccgggat gtttaccttg aacttttctg caccaatacc gaggggcgag tcgttgccag
     9001 cagcgatccg gccagcatcg gcagcatcag gccccagggg gaaggtatat tgctcaccgg
     9061 cgacacgaat gtaccgatcc ggctcggcac tctgcaactc accggaacag atggctccgc
     9121 agtgctgcct atctcggcca acgtaccgtc catgttcaat gatggccaga tcggcgagct
     9181 gcaactactg ttcaactggg cgcagatata ccgcatcctg gatcaatccg cacaaagcgg
     9241 gcacgaactg gcgctactcg atccggaccg caggatcatt gccgcatcat ccaaactgcg
     9301 ggagcgcaac ctgttgcagc agcaggttcc tgcaaactgg atcgctagcg gcagaaccgg
     9361 catcggcacc tacgatggca ggcttctggg catgaacgag gtcatggtcg gatacgaccg
     9421 ctcgccgggg ttccagcagt ttccggggtt tagctggacc acactggtga tcgaaagcca
     9481 tcatcaggcc ttcatcccgg tacgccagat ggcactggta ttcctgctgc tgctggcact
     9541 gaccagtatc gtcgccatcg ccttctcgct gctggtggcc ggccgtatcg cccgcccgat
     9601 tgccgcgctg actgaattca cgcgccgttt caagcgcgac caacagttgc cggaagcccc
     9661 gcaggccatc ggcggcgaag tgggggaact gaccgaggcc ttcgtgcaga ccgtgcgcga
     9721 cctcgaccag tcgcgcaccg acctggtacg cgcttccaag ctcgccgttt tgggagaaat
     9781 ggcggcagtg atggcgcacg agatccgcac ccccatcggt atcctgcgct cttccgccca
     9841 gatgctggca cgcgaaccgg agctcagacc agaagcgcag gagctgaccg gcttcatcga
     9901 gagcgaaacc gaacgcctca accgcctggt ctccaccctg ctcgacagcg cccgtccacg
     9961 cgcgccgaaa ttgcagccgg ccgacctgca cgctatcatc cgccacagca tcgatctgct
    10021 ggcggcacag gcagacaaga aaaacatcca catccacctc aaactgtatg gccgccaccc
    10081 cgtgatcgag ctggatgccg aacagatgac ccaggtgctg ctcaacctgg tactcaacgc
    10141 actgcagatt ctgccgcaag gcgggcaggt ggaaattacg acacgtgaag atgccggaaa
    10201 actggtgttg gaaatcgccg acgacggccc cggcattccg ccgcaggaac tggttcgcgt
    10261 gttcgacccc ttcttcacca aacgcgaagg cggcgtgggc ctcgggctgg ctgtggtgca
    10321 acagatcgtc gctgcgcatg gcggcgaaat ccacgccggc aacagtgcgc tcggcggcgc
    10381 gctgtttacc atcactttgc cgatgaaaga aagttcatga ccggtcagca cgttctggta
    10441 gtcgacgacg aagcgaaaat gcagcgcgtg ctggaaatca tgctcaagcg tatggggcac
    10501 gcggtcgcct ccgccagcaa cggtcgtgaa gcccttcaga tccttaagtc ctcacccgtc
    10561 gacctgatca tcagcgattt gcgcatgccg gaaatgagcg gcaccgaact gctgcaggcg
    10621 ctgcgccaac agggcaacga ggtgccggtg atcatcatga ctgcctacgg caccatcgaa
    10681 agcgcggtcg aggcaatgaa actgggcgcg tgcgattaca tcgtgcggcc attcgatgtg
    10741 gaggcactgg aaatcgccat caaccgcatc ctggcggcag gccgcatgcg ccgacaaaac
    10801 gatttcctgc gacatgaagt ggacaagggc tggggtgaat tcattggcaa cagcccgcca
    10861 atgcaaacaa tctatgatct catccgccag gtggcgccag gaaaaactgc ggtgctcatc
    10921 acgggagaaa ccggcactgg caaagaactg gtggcgcgcg ccatccatcg cgcctcgccc
    10981 cgccatgaag ctctgttcgt accaatcaac tgcgccgcca ttcccggcga catcctggaa
    11041 agcgagctgt tcggctatga aaaaggcgcc tttactggtg ccagcaagga ccgtatcggc
    11101 aagttcgaaa tggccgatgg cggcaccctg tttcttgacg agctcaccga aatgccaatg
    11161 gcactgcaag ccaaactgct gcgcgtactg caggaaaacg tcatcgaacg gctgggcagc
    11221 aaccgcgtca tcccgatcga cgtgcgcatc gtcaccgcca ccaaccgtga cccacgtcag
    11281 gctatcgccg agggtaagct gcgtgaagac ctgtattacc gggtcaacgt gttcaacatt
    11341 gacctgccac cgctacgcga acgcaagcag gatatcccgg ccctggtggc cggcttcctg
    11401 tccggacacg ccggaaccca tggcatctcg gaagccgcac tggcccgcct gcaggactat
    11461 gcctggcccg gcaacgtacg tgaactgcag aatgtcatcg aacgtgccgc agtgctgagc
    11521 cgtggcagcg tcatccagat ggaacacctg ccgcgcgaaa tgacaacggc cgatgcagaa
    11581 tcggtgattg ccgaatcccc gcaatttcac agcctggaac tcaatccggc ggtggaaaat
    11641 ctggaacgca cgatgatacg cgccgcgctc ctgcaaaccg gcggcaacaa gagcaaggcg
    11701 gcacgcttgc tggatatcag cgaacgttcg ctgtggtaca aaatcaagaa ttacgggttg
    11761 gaatagtcgc cttgcctgcc gaacccctgt tcacaaaacc tgccgtactc tcggtcagcg
    11821 acctcaatcg cctggcgcgc gaagtgctgg aacacagctt cccgctgttc tgggtggcgg
    11881 gcgagatttc caacctgacc cgggccgcct ccggccactg gtatttttcg ctcaaggatg
    11941 acaaggcgca ggtgcgctgc gtcatgttcc gcaacaagaa catgctcctg gactggcagc
    12001 ccaaggaagg cgaccacacc gaggcacgcg ccctggtcac gctgtacgag gcgcgcggcg
    12061 atttccagct caacatcgaa gccctgcaac gcgccgggct gggcgcgctg ttcgaggcct
    12121 tcgagcggct caaggccaga ctggagtacg aaggcctgtt cgatgctg
//
LOCUS       DAOMFA010000034        12147 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_508_length_12147_cov_5.034816, whole genome shotgun sequence.
ACCESSION   DAOMFA010000034 DAOMFA010000000
VERSION     DAOMFA010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 12147)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12147)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12147
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_508_length_12147_cov_5.034816"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(9..254)
                     /locus_tag="PKW44_03015"
                     /pseudo
     CDS             complement(9..254)
                     /locus_tag="PKW44_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441483.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
     gene            322..1299
                     /gene="birA"
                     /locus_tag="PKW44_03020"
     CDS             322..1299
                     /gene="birA"
                     /locus_tag="PKW44_03020"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HQR50594.1"
                     /translation="MNSLTFPILRLLADGQFHSGEAIARHFGVTRATVWNALQDVEVL
                     GVKLFSVRGKGYRLPQPVRFLEQDKVMAALGGAAGKFTLELHDRIDSTNSYLMQLAAG
                     GHPHGACVAAELQTSGRGRRGRAWQAGLGNSLTFSVLWRFDCGAGALSGLSLAVGVAL
                     MRALRELGVTGAGLKWPNDVLHDKQKLAGILIELQGDMDGPTAAVIGIGLNLRLPETL
                     RKNIDQPATDLQSILGQPPDSAQGLGTMLRHLAVTLEQFDHEGFEALREEWIAHHAFD
                     GQYVRIHMPDGRQIDGVAEGIAADGALLLRTEHGTQRFAAGEVSLRSAA"
     gene            1296..2027
                     /locus_tag="PKW44_03025"
     CDS             1296..2027
                     /locus_tag="PKW44_03025"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HQR50595.1"
                     /translation="MILAIDAGNTRTKWGVFDAAGERVAQGVLENTQLDSLAQKNAAW
                     AGCCRAVVSNVAGVAVERQLRSLLDGFQLPALWVKAEAAGCGVTNGYTHPGQLGPDRW
                     AALLAAWNRYREPCVVATAGTALTVDALSAKGEFLGGLIVPGLHMMQAALAAGTAALP
                     LAEGNLCEYPTGTAAAIYSGGINAMAGAVERMRARLEERESRPPRCLLSGGDAVRLAE
                     AIHGPSEIVDSLVLQGLYLIEREHS"
     gene            2024..2686
                     /locus_tag="PKW44_03030"
     CDS             2024..2686
                     /locus_tag="PKW44_03030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR50596.1"
                     /translation="MKWIAWLLLIANVVLFGYFKLSAPQPVGVVVSQQPIHPEQLRIL
                     TPEQLAALPNKSSPPAPAPEPVLQTACYEWASFSGANIARARAALDQFSLDTTVRQTA
                     PHEAARYWVYIPPRKNLEEALAKNDELHAMGINETFVVQEPQWRYAISLGIFKDEKLA
                     NRLLDDVRARGVSIAVKGVRNQEKGQSGFFIKNVTASVADEIRKLQPDFPGSELKQVD
                     CQ"
     gene            complement(2839..4206)
                     /locus_tag="PKW44_03035"
     CDS             complement(2839..4206)
                     /locus_tag="PKW44_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HQR50597.1"
                     /translation="MSSTLFLDRLRSLLPADRLFTDPVDCYAYAYDNSRNFRPPLAVA
                     FPLTTEDVQAVVRLCHEHRIPLVPRGRGTGTAGGSVPEQGGVALSLERMNRILKFDPD
                     NRMAIIEPGVLNQELQDALKPAGFFWPPDPSSAPYCSVGGNLATCAAGPHAVKYGVAR
                     DHVLGLKAVTGSGELIKTGCYTTKGVVGYDLTRLIVGSEGTLAVIVEATLKLTPLPRA
                     TGGITAQYRDTTSCAHAIAAIMAQPFVPSALEFLDNNALNLIRNRHPGMLPEDMRALL
                     MIEVDGSQSEVAEATEAVIQTCRVDGLIEAKEAADTKSLWAARKALSPLLRDVAPKKI
                     NEDVAVPVSRLPELLGGIEQLAAHHRIANVNFGHAGNGNIHVNLLVNPDNGDEMQRAE
                     ACLDEVFSLVLSLDGTLSGEHGVGMAKRPFVPREIDATTISLMKSVKQAFDPNNILNP
                     GKLFP"
     gene            complement(4258..4857)
                     /gene="recR"
                     /locus_tag="PKW44_03040"
     CDS             complement(4258..4857)
                     /gene="recR"
                     /locus_tag="PKW44_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HQR50598.1"
                     /translation="MKNPPALEHLVESLRCLPGVGPKSALRMAYHLLQRDRPGAASLA
                     GALQNALEQVGHCSLCNNFSEEPVCNLCATGKRDARLLCVVEMPTDLLMLEQTHTYHG
                     MYFVLMGRLSPLDGIGPREIHLDRLLKRAQDGVVEEVILATNYTVEGEATAHYISELL
                     KTRGIKVSRIARGLPMGGEIEHVDSGTLVQAMMERRTLR"
     gene            complement(4868..5191)
                     /locus_tag="PKW44_03045"
     CDS             complement(4868..5191)
                     /locus_tag="PKW44_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HQR50599.1"
                     /translation="MKGGLGNLMKQAQQMQENMKRAQEELGSVEVEGQSGAGLVKVVM
                     TCRHDVKRVKIDDSLLGEDKDMLEDLVAAAVNDAVRKAEATSQEKMAGMMDGMQLPPG
                     MKLPF"
     gene            complement(5205..6851)
                     /gene="dnaX"
                     /locus_tag="PKW44_03050"
     CDS             complement(5205..6851)
                     /gene="dnaX"
                     /locus_tag="PKW44_03050"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HQR50600.1"
                     /translation="MSYQVLARKYRPRTFQELVGQEHVVRALSNALEQQRLHHAYLFT
                     GTRGVGKTTLARILAKALNCETGITATPCGQCTACTEIDRGRFVDLLEVDAASNTKVD
                     QMRDLLENAQYAPTVARFKVFIIDEVHMLSTSSFNAMLKTLEEPPAHVKFILATTDPQ
                     KMPVTVLSRCLQFNLRQMPAASITQHLHAVLDKENIPAESVALQLLARAASGSMRDAL
                     SLLDQAIAYGGGAVHEVEVRAMLGAIDQNYLFTLLDALSRQDGPALLAVAQNMEERSL
                     SFDAALADLALLLHQIALTQSIPDAIPEDLPERAQLTQLATQFSTEDLQLYYQIALLG
                     RRDLGLAPDEFAGFTMCLLRMLAFTPGETPARPAVPPPTPLKAVLTTAQPVPAAPEPV
                     SLPEERFAEKPQAAQQTVVAPPSAEPVPPAFDGNWRSLVDSLKLGGMARMLAQNCELA
                     EFDGQRMRLVVPQNQKHLLDSTTQEKLRAVLKERFGKQLQMQIEIGGAAANTPAQQIS
                     QEKAARQAQAEASINNDQFVRDLMDGFGATIIPSSIKPIQ"
     gene            complement(6902..6999)
                     /gene="ffs"
                     /locus_tag="PKW44_03055"
     ncRNA           complement(6902..6999)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PKW44_03055"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            7290..7763
                     /locus_tag="PKW44_03060"
     CDS             7290..7763
                     /locus_tag="PKW44_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019918660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HQR50601.1"
                     /translation="MFKSLSINPEKCTGCLQCEMACSYEHEGVFNTAKSRIKVFDFHH
                     EGRKVPYTCTQCAEAWCMQACPVDAIRLDGALGAKMVYDDVCVGCKVCTIACPFGTIN
                     YNSDTGKVMKCDLCGGDPQCASACPTGAITYVDSDWTGLEKMRKWAAATDTAPRA"
     gene            7782..9632
                     /locus_tag="PKW44_03065"
                     /pseudo
     CDS             7782..9632
                     /locus_tag="PKW44_03065"
                     /inference="COORDINATES: protein motif:HMM:NF013481.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
     gene            9636..10886
                     /locus_tag="PKW44_03070"
     CDS             9636..10886
                     /locus_tag="PKW44_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019918658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQR50602.1"
                     /translation="MDYVIIGNGPAGIIAAETLRKADAVGKVAVVGAEPETPYSRMAI
                     PYLVAGNIPLEGTYLRKDREHYRRLGIELLHGRVAAVDAAAKTLRFEDGRQRPYDKLL
                     IATGSSPAQPPIPGMDLSNIHACWTLEDARRIIAAVKPGTRVLQMGAGFVSCIVIDPL
                     RKRGAKLTIIGSGDRMVPRMMTPGASKLIQKWVERNGVKVYNNLRVDALRAGPNGTVI
                     ASLHSGEEVETDLVINATGVKANIGFLEGSAIATQYGVLVDEYLCSSQADVYAAGDVA
                     QARDIATGGKTVNAIQPNAVDQGRIAALNMAGKPVAFQGGIAMNVLDVGGLVSASFGR
                     WWGADGGEHVELMDEDGWRYLRLEFKDEVMVGATAVGLTQHVGVLRGLIQAQVALGEW
                     KQHLLKDPTRIMEAYLAKAQAATI"
     gene            10886..11137
                     /locus_tag="PKW44_03075"
     CDS             10886..11137
                     /locus_tag="PKW44_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018229610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HQR50603.1"
                     /translation="MKVTLKLFASLRDYLPEGSTSAILELEVPEGTTPADLAERYQLP
                     PRLVHLVLVNGHYVPPENRAGTALVEGDVLAIWPPIAGG"
     gene            11158..11421
                     /locus_tag="PKW44_03080"
     CDS             11158..11421
                     /locus_tag="PKW44_03080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR50604.1"
                     /translation="MTITEAEFLRLLAKAIAPLPFSLGKNVATVAIGAGAVRIAFHGA
                     PQQPLGSLMLPMTQVDIWFDDVDETTASRFLDRFDRAFHRGGG"
     gene            11509..>12147
                     /locus_tag="PKW44_03085"
     CDS             11509..>12147
                     /locus_tag="PKW44_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR50605.1"
                     /translation="MLTVIKAGKNGLAQSLRKWKDPTAQQLGSVILALLVLSVSLISA
                     FWILAFSELGEGYAFMSVFPYSYLIISYASLLLFYRIKSVEYFTFTQLTMLLVMPFFM
                     QWVIGGFTASSGVAIWGILSPVGALMILGTRQSTPWFLLFSLLAMISWGLDSTFAKAA
                     LPIPKHTKEVFFLINVCGTAAILYFVMRFFESQKERLMREVDEKNRLLEQEQE"
BASE COUNT         2307 a         3424 c         3908 g         2508 t
ORIGIN      
        1 cctgcttact ccgctgccag gccgaagttg aggcagatgc ctttggcgtg gccgatgtga
       61 aggtagccat tggggttggg tgggaatcgc ttgcggatcc tggctgggtc gggctggccg
      121 gcggcctggt gcgcggcatc gccggggctt ccggcccagc ggcggctggc ataggtgccg
      181 gcggccaggt cgcggtcaat aatgccgcgc aaaaaattgg aagcgggagc gggggtgttt
      241 ttttcgtcgg acatggtcga ttgcaatgga ttgatttgcc gccattttac actgtggcga
      301 ggcgggtata attgcctgta catgaattcg ctcacatttc ccatcctgcg cctgctggcg
      361 gacggccagt tccattctgg ggaggccatt gcacgccact tcggcgtgac gcgggcgacg
      421 gtgtggaacg ccttgcagga tgtcgaggtg ctgggcgtca aacttttttc cgtccgcggc
      481 aagggctacc gcctgccgca acctgtgcgc ttcctggagc aggacaaggt gatggcggcg
      541 ctgggcggcg cagctggcaa attcacgctg gaactccacg accgcatcga ctccaccaat
      601 agctatctga tgcagctggc agctggcgga catcctcacg gtgcatgcgt ggcggcggaa
      661 ttgcagacca gcggtcgcgg ccgtcgcggc cgcgcctggc aggcaggtct gggcaacagt
      721 ctgacgttct ccgtgctgtg gcgcttcgat tgcggcgccg gcgcgctttc cgggctcagc
      781 ctggccgttg gggtggcgct gatgcgcgcc ttgcgagaac tgggcgtcac gggggccggg
      841 ctgaaatggc ctaacgatgt actgcacgat aaacagaaac tggccggcat cctgatcgag
      901 ctgcagggtg acatggatgg tcccaccgcg gcggtgatcg gcattggcct caacctgcgc
      961 ctgccggaaa ctttacgaaa aaacatcgac cagcctgcca ccgatttgca atccatcctc
     1021 ggccagccac cggattccgc gcaagggctg ggcaccatgc tgcgccacct ggccgttacg
     1081 ctggaacagt tcgaccacga aggcttcgag gcgctgcgcg aggaatggat agcccaccat
     1141 gccttcgatg gacagtacgt gcgcatccat atgccggatg ggcgacaaat cgacggtgtg
     1201 gcggaaggca tcgctgccga cggtgcgttg ctgctgcgca cggaacacgg cacacagcgt
     1261 tttgccgccg gcgaagtcag tttgcggagc gcggcatgat cctcgcaatc gatgccggca
     1321 acacccgcac caagtggggt gtgttcgacg ctgccggaga gagggtggcg cagggcgtgc
     1381 tggaaaatac gcaactcgac tcgttggcgc agaaaaacgc ggcctgggcc gggtgttgcc
     1441 gagcggtggt gtccaacgtc gcgggggtgg cggtggaacg gcagttgcgc agcttgctcg
     1501 atgggtttca attgccggca ctgtgggtca aggccgaagc ggctggctgt ggcgtcacca
     1561 acggctatac gcaccccggg caactcggcc cggatcgctg ggcggcgctg ctggcagcct
     1621 ggaaccgtta ccgcgaaccc tgtgtggtgg cgacggcagg aactgcgctg acggtagatg
     1681 ccctgtcggc aaagggcgag tttctgggcg gcttgattgt gcccggcttg cacatgatgc
     1741 aggctgcgct ggcggcaggc acggcggcgt tgccactggc ggagggcaat ttgtgcgaat
     1801 atccgaccgg caccgccgcc gccatttatt ccggcggcat caatgccatg gccggggcgg
     1861 tggagcgcat gcgtgcccgg ctggaagagc gcgaaagccg cccgccgcgc tgtctgttgt
     1921 ccggtggcga tgccgtacgt ttggcggagg cgatacacgg gccgtccgag attgtcgata
     1981 gtctggtgtt gcagggcttg tatttgatcg aaagagaaca ctcatgaaat ggattgcctg
     2041 gttactgctg attgctaatg tcgtgctgtt cggctatttc aaattgagcg cgccgcagcc
     2101 cgtcggtgtt gtagtcagcc agcagccgat ccacccggaa cagttgcgga tcctgacacc
     2161 ggaacagctt gcggcattgc cgaacaagtc ttcgccgcct gcgccagcgc ccgagccggt
     2221 gttgcagacc gcctgttatg agtgggccag cttttccggc gccaatatcg cgcgtgccag
     2281 ggcggcgttg gatcagttct cgctggatac gactgtccga cagacagcgc cgcatgaggc
     2341 ggcacgctac tgggtttata ttccgccacg caagaacctg gaggaggcgc tggcgaaaaa
     2401 tgacgagttg cacgccatgg gtatcaacga aactttcgta gtgcaggagc cgcaatggcg
     2461 ttacgctatc tcgctaggca tcttcaagga tgaaaagctg gctaacaggc tgctcgatga
     2521 tgtgcgcgca cgtggcgtca gcatagccgt caagggcgtg cgcaaccagg aaaaaggcca
     2581 gtccgggttt ttcatcaaga atgtcacggc cagtgtggct gacgaaatca gaaagttgca
     2641 gccggacttt cccggcagcg aactcaagca ggtagattgc caataggagg agatatggga
     2701 atgctcagtt cggccgtgaa aagcatggcc aaaaaagtgg cggtgaccgc tgcattggtc
     2761 atcggtgaac gcgttgccag gaagatcctc gacaaggttg cggaaaagcg ggggaaaccg
     2821 tcccccgcgc cgggtgaatc agggaaatag ttttcccggg ttgaggatgt tgttcgggtc
     2881 gaacgcctgt ttgactgact tcatcaggct gatcgtcgtc gcatcgatct cgcgtggcac
     2941 gaaggggcgc ttggccatgc ccacgccatg ctcgccggaa agggtgccat ccagcgacag
     3001 taccagcgag aacacttcat ccaggcaggc ttcggcgcgc tgcatctcgt cgccgttatc
     3061 cggattcacc agcaaattta catggatgtt gccgttgccg gcgtggccga aattgacgtt
     3121 agcgatgcgg tgatgcgccg ccagttgttc gatgccgccc agcagttccg gcaggcgcga
     3181 aaccggcacc gccacgtcct cgttgatctt cttcggcgcc acgtcgcgca atagcggcga
     3241 cagcgccttg cgcgccgccc acagtgactt ggtgtctgct gcctctttgg cctcgatcag
     3301 gccatctacc cggcaagtct ggatcaccgc ttctgtcgcc tcggccactt cgctttgcga
     3361 accgtccacc tcgatcatca gcagggcgcg catgtcttcc ggcagcatgc cggggtggcg
     3421 attgcggatc aggttgagcg cgttgttgtc gaggaattcc agcgctgacg gcacgaacgg
     3481 ctgcgccatg atggcggcga tggcatgggc gcaactggtg gtatcgcggt actgggcagt
     3541 aatgccgccg gtggcgcgcg gcagcggggt cagcttgagc gtggcctcga cgattaccgc
     3601 cagcgtgcct tccgaaccga cgatcagtcg cgtcaggtca tagcccacca cgcccttggt
     3661 ggtgtagcag ccggtcttaa taagttcgcc gctaccggtg acggctttca gcccgagtac
     3721 gtggtcgcgc gccacgccat acttgacggc atgcggcccg gcggcgcagg tggcaaggtt
     3781 gccgcccacg ctgcaatacg gcgcgctgga ggggtcgggc ggccagaaaa agcctgcggg
     3841 cttcaatgca tcctgcaatt cctggttgag cacgcccggc tcgataatcg ccatgcggtt
     3901 gtccgggtcg aacttgagga tgcggttcat gcgttccagc gacagcgcca cgccgccctg
     3961 ttccggtacg ctgccgcctg cggtgccggt gccgcgacca cgcggcacga gcggaatgcg
     4021 atgctcgtga cacagtctga ccacggcctg cacgtcttcg gtcgtcagtg ggaaagccac
     4081 tgccagcggt ggcctgaaat tgcgtgaatt gtcataagcg taggcgtagc agtccaccgg
     4141 atcggtgaac agccggtcgg ccggaagtag cgacctcagg cgatcgagaa aaagggtgga
     4201 tgacatatca atatccgcga tgcatttacg atcttgttca atccgggttt gaaagcgcta
     4261 tcgaagcgtc cgtcgttcca tcatggcttg caccagtgtg ccgctgtcca cgtgctcgat
     4321 ttctccgccc atgggcaggc cgcgcgcgat gcggctgacc ttgataccgc gcgtcttcag
     4381 cagttcgctg atgtaatgcg cggtggcttc gccttccacg gtatagttgg tggcgaggat
     4441 gacttcttcc accacgccat cctgagcgcg cttgagcagg cgatcgaggt ggatttcccg
     4501 ggggccgatg ccgtccagtg gagacagccg acccatcagc acgaagtaca tgccgtggta
     4561 ggtatgggtc tgctccagca tcagcaggtc ggtaggcatt tccaccacgc acagcaggcg
     4621 tgcatcacgc ttgccggtgg cgcacagatt gcagaccggc tcctcgctga agttgttgca
     4681 caggctgcaa tggccgacct gttccagcgc attctgcagc gccccggcaa gactggcggc
     4741 gccggggcgg tcgcgctgca gcaggtggta ggccatgcgc agcgcagact ttggcccgac
     4801 gccaggcaag cagcgcagcg attcgaccag atgttccagc gccggcgggt ttttcatatg
     4861 tggaggctca gaacggcagt ttcatccccg ggggcaactg cataccgtcc atcatgccgg
     4921 ccattttttc ctgcgatgtc gcttccgcct tgcgtacggc gtcgttgacc gcggccgcca
     4981 ccaggtcttc cagcatgtcc ttgtcctcgc ccagcaggct gtcgtcaatc ttgacgcgct
     5041 tgacgtcgtg gcggcaggtc atgacgacct tgaccagtcc tgcgccggat tggccctcga
     5101 cctcgacgct gccaagttct tcctgcgcgc gcttcatgtt ttcctgcatt tgctgggcct
     5161 gcttcatcag gttgcccaag ccgcctttca tcatggtttt gctcctattg aatcggtttg
     5221 atactggatg gaatgatagt ggcgccaaac ccgtccatca ggtcgcgcac gaactggtcg
     5281 ttgttgatgg aggcctcggc ctgcgcctgg cgcgcggcct tttcctggct gatctgctgc
     5341 gccggggtgt tggcggcggc accgccgatt tcgatttgca tctgcagttg cttgccgaag
     5401 cgttccttca gcacggcacg cagtttttcc tgggtggtgc tatccagcag atgtttctgg
     5461 ttctgcggca cgacgagacg catgcgctgt ccgtcgaatt ccgccagttc gcagttctgt
     5521 gccagcatgc gtgccatgcc gcctaacttg aggctgtcta ccaggctgcg ccagttgcca
     5581 tcgaatgccg gtggcacagg ctcggcggaa gggggggcga cgacggtctg ctgggctgcc
     5641 tgcggctttt cggcaaagcg ttcttctggt aaagaaacag gttccggcgc tgcgggtacc
     5701 ggttgtgccg tggtaagcac cgccttgaga ggggtgggcg gcggcacagc cggcctggcc
     5761 ggcgtttccc ccggcgtaaa tgccagcatg cgcagcaaac acatggtgaa gccggcaaac
     5821 tcgtccggcg ccaggccgag gtcgcggcgg ccgagcaggg caatctggta ataaagctgg
     5881 agatcttccg tgctgaactg cgtggccaac tgggtcagtt gcgcgcgctc cggcaagtct
     5941 tccgggatcg cgtccggaat ggactgcgtc agtgcgatct ggtgcagcag cagtgccaga
     6001 tccgccagcg cggcatcgaa tgacaggctg cgctcctcca tgttctgcgc aacggccagc
     6061 agggcagggc catcctgacg cgaaagggcg tcgagcaggg tgaacaggta gttctggtcg
     6121 atagcaccca gcatggcgcg cacttcgact tcgtgtactg cgccgccgcc gtaggcgatg
     6181 gcctgatcca gtagcgacag ggcgtcgcgc atgctgcccg aggcggcgcg tgccagcagc
     6241 tgcagggcga cgctttccgc cgggatgttt tccttgtcca gaacggcatg caggtgctgc
     6301 gtgatggagg ccgccggcat ctggcgcagg ttgaattgca ggcagcgcga cagcacggtg
     6361 accggcatct tctgcgggtc ggtggtggcg aggatgaact tgacgtgcgc cggtggctcc
     6421 tccagcgtct ttagcatggc gttgaaggag gaggtggaca gcatgtgcac ttcgtcgatg
     6481 atgaacacct tgaagcgcgc cacggtcggc gcgtactgcg cgttctccag caggtcgcgc
     6541 atctggtcga ccttggtgtt ggaggcggcg tccacttcca gcaggtcgac gaagcgtccg
     6601 cggtcgattt cggtgcaggc ggtacattga ccgcaaggcg tcgcggtgat gccggtttcg
     6661 caattgagtg ccttggccag gatacgcgcc agcgtggttt tgccgacgcc gcgcgtgccg
     6721 gtgaacaggt aggcgtgatg cagccgctgc tgttccagcg cgttgctgag ggcgcgtacc
     6781 acatgctcct ggccgaccag ttcctggaat gtcctggggc gatacttgcg cgcgagaacc
     6841 tggtagctca tggggttttc gatggattgg taaagcctga actttagcct aaaacaaggg
     6901 aaggggcgag cctaaccccc ggcacttgct tcaatagttg tggctgcttc cttccggacc
     6961 tgaccaggtt ggctaccttg caatgcgggg aggcccgccg gtaggtattc taaccgatca
     7021 ccgaagttaa tgacattgcc ttgcgtgcgt ttctctatat tctttcagaa ataaccaaat
     7081 tctgccggat atccattctt tcgttatatt tttaaataaa tatcgtaacc atgactcaat
     7141 aaaaactaat taaacaattg gttatagcgc cttcatattg tgtttgacgc acacttttca
     7201 ttaatctaac ctagccatat acaaaagaaa tgcggcttat agacgctcgc attattcagg
     7261 gaattttagg gttaacgagg agatttccaa tgtttaagtc attgtccatc aatccggaaa
     7321 aatgcacggg ttgtctgcaa tgtgaaatgg cctgttccta cgagcacgag ggtgtgttca
     7381 ataccgccaa gtcgcgcatc aaggtgttcg atttccatca tgaaggtcgc aaggttccct
     7441 atacctgtac ccagtgcgcc gaggcctggt gcatgcaggc ctgtccggta gatgccatca
     7501 ggctggatgg cgcgctcggc gccaagatgg tgtacgacga cgtctgtgtc ggctgcaagg
     7561 tctgcaccat cgcctgcccg ttcggtacca tcaattacaa cagcgatacc ggcaaggtga
     7621 tgaagtgcga cttgtgcggc ggcgatccgc aatgcgcatc cgcctgtccg accggcgcga
     7681 ttacctatgt ggactcggac tggacaggtt tggaaaaaat gcgcaagtgg gcggcggcga
     7741 cagataccgc accgcgtgct taaaggacga ggagaaatat catggcatgg catcgtaaag
     7801 ttcttcgggt cgacctgacc aacggcacct gcgccgccga accgctcaat atggaatggg
     7861 ctgatcagta ccttggccag cgcggtttgg cgaccaaata tttctgcgag gaagtcgacc
     7921 cccgggtgga tccgctttcc cctgaaaaca agctgatcta taccacaggc ccattgaccg
     7981 gcaccatggc ctccacgggt ggccgttatt ccgtggttac caaaggccca ttgactggcg
     8041 cgatcgcctg ctccaactcg ggcggctatt tcggcgctga actgcgcttc gccgggtggg
     8101 acatgattgt cctggagggt aagtcgccca gaccggtcta cctgcacatc gagaatgaca
     8161 aggccgaact gctgccggcg gaggatttca tctggggcaa gagcgtttgg gatacagagg
     8221 aactgatcaa gacccgtcat caggatccgc tcatgcgggt ttcctgcatc ggcaaggccg
     8281 gtgagaacgg cgtattgttt gccgccatcg tgaatgacct gcatcgagct gccggccgtt
     8341 ccggcgttgg gacggtgatg ggttccaaga acctcaaggc tgtggccgta cgtggcacca
     8401 agggggtagg gggaattgcc gatcccaagg cgttcatggc ggcgacaaag gctggcaaaa
     8461 aggtgttgca cgacaacccc gtgaccggcc aggggctgcc caagtatggt actcaggtgt
     8521 tgatgaacgt gatcaacgaa atgggcgcgt ctccgacgcg taaccatcag gacgtgcagt
     8581 tcgagggggc ttccaggatt tccggcgagg ccatgcacga aaaacgtgcg acggatggca
     8641 agactcacct ggtgacgaac caggcctgtt tcggttgcac cattgcctgc ggccgtattt
     8701 caaaaattga cgagacccac tattcggtca agacccgtac ggaatagtgg ggtgccaatg
     8761 gcggcctgga atatgaggct gcgtgggcgt tcggcaatgc caatggcgtg gatgacctcg
     8821 aagccctgca attctgcaat ctgctgtgca acgaggaggg aatggacccg atcaccctgg
     8881 gggcgacggt cggcgccgtg atggaattgt atgaaatcgg tgcgctgagc aaggaggaaa
     8941 tcggcttcga agcgccattc gggtcgacgg atgcactgct caagctggtg gagatgacgg
     9001 ccaggggaga aggcttcggc aagatcgtcg ggcttggttc caagcggctc accgcaaagt
     9061 acggccaccc agagctatcc atgtcggtga aggggcagga atttccagct tacgatggtc
     9121 gtgccctgca aggcatgggg cttgaatacg ctacctccaa ccgcggggcc tgccacctgc
     9181 gcggctacac ggttgcctca gaggtactgg gcattccttt caagactgag ccgcaagcga
     9241 ccgagggcaa gccggaattg ctcaaggcgt ttcaggatgc caccgcggtg gtggattctg
     9301 ccggcatctg cgtgttcacg acgtttgcct ggtcgctgga ggatatccag ccgcagattc
     9361 aggcggcttg cgagggcgac tggtctatgg acaagctgaa tgtcatgggc gaacgcatct
     9421 ggaatatgga gcgcgagttc aacctgaggg ccggttttac cgccaaggac gacactctgc
     9481 cgccgcgcct gctcaaggaa gggtgtaaga ccggtccgca gaagggcgcc gtcgcgcgcc
     9541 tggacgttat gatgccgaaa tactatgaag tgcgcggctg ggatgccgaa ggtgtgccaa
     9601 cggcggaaac caaagcaaga ttgggattgt agccaatgga ttacgttatt atcggcaacg
     9661 gcccggccgg aatcattgca gcggaaacgc tgcgcaaggc tgatgccgta ggcaaggtcg
     9721 ctgtggttgg cgccgaaccg gaaactccct attcccgcat ggcaattcct tatttggttg
     9781 ctggcaacat tcctctcgaa ggcacctacc tgcgcaagga ccgcgagcat taccgtcggc
     9841 ttggcattga gttgctccat gggcgtgtgg ccgcagtcga tgcggcagcc aaaacgctgc
     9901 ggttcgagga cggacgtcaa cggccttatg acaagctgct gatcgctacc ggctcctcgc
     9961 cggcacagcc accgattccg ggcatggatc tgtccaacat tcatgcctgc tggacactgg
    10021 aagacgcgcg ccgcatcatc gccgctgtca aaccggggac gcgcgtgctg caaatggggg
    10081 caggtttcgt cagttgcatc gtgattgatc cgctacgcaa gcgcggtgca aaacttacta
    10141 ttatcggctc cggagaccgc atggtgccgc ggatgatgac gccgggcgcc agcaaactga
    10201 tccagaagtg ggtcgagcgc aatggagtca aggtctacaa caacctgcgg gtggatgccc
    10261 tcagggctgg tccgaatgga acagtcattg ccagcctgca ttccggcgaa gaagtggaga
    10321 ccgacctcgt gatcaacgcc accggggtca aggctaatat cggttttctg gaaggcagcg
    10381 ctatcgccac gcaatacggc gtactggtgg atgaatatct gtgcagcagc caagcggacg
    10441 tctatgcggc cggcgatgtg gcgcaggcta gggatatcgc taccgggggc aagaccgtta
    10501 acgccatcca gcccaatgcg gtggatcagg ggcgtatcgc ggcgcttaac atggcgggaa
    10561 agccggtggc gttccaggga ggcattgcga tgaacgtgct ggatgtcggc ggcttggttt
    10621 cggcttcgtt cggccgctgg tggggcgccg acggtggcga acatgtcgaa ctgatggacg
    10681 aggacggctg gagatatttg cggctggaat tcaaggatga ggtgatggtg ggggccaccg
    10741 cggtcggctt gacccagcat gtcggcgtgc tgcgcggcct gatccaggcg caggtggcgc
    10801 tgggcgagtg gaagcagcat ctgctcaagg atccgacccg catcatggaa gcctatctgg
    10861 ccaaggcgca agcagccacc atttaatgaa agtcacactc aagctcttcg ccagcttgcg
    10921 ggattatctt cccgaaggaa gtaccagcgc gatccttgaa ctggaagtgc cggaaggtac
    10981 tacgcctgct gatctggcag agcgctatca attgccgccc agactcgtgc atctggtatt
    11041 ggtcaatggc cactacgtgc cccccgaaaa tcgtgccggc accgccttgg ttgaaggcga
    11101 tgtgctggcg atctggccac ccatcgcggg aggatgatgc ctgtcgtttc acggtcgatg
    11161 accattaccg aggcggagtt tctgcggctg cttgccaagg cgatagcgcc attgccgttt
    11221 tccctcggta aaaacgtggc taccgtagcc attggggcag gagccgtgcg gatcgcattt
    11281 catggagccc cgcagcagcc attgggcagc ctgatgctgc cgatgacgca agtggacatc
    11341 tggtttgacg atgttgacga gacgactgcc agcagattcc tcgatcgttt cgatcgcgcc
    11401 tttcaccgcg ggggaggcta gttcggtggc ttccggaggg cagttgcgct atagttcggc
    11461 catgcacgca agcgaaagaa cacgacaatt gccccgtccg gatccggcat gttgacggtc
    11521 atcaaggcgg gtaagaacgg cctggcgcaa tctcttcgca agtggaagga ccctactgcg
    11581 cagcagttgg gcagcgtcat actcgctttg ctggtgttgt ccgtcagcct gatcagcgca
    11641 ttctggatcc tcgcgttctc agaactgggc gaaggctatg ccttcatgtc agtgtttccc
    11701 tattcctacc tgatcatttc ctacgccagc ctgttgctgt tctaccggat caagagcgtc
    11761 gagtacttca cctttaccca gctgaccatg ctgctggtca tgccgttttt catgcagtgg
    11821 gtgatcggcg ggttcacagc ttccagtggg gtggcgatct ggggcatcct gtcacccgtc
    11881 ggggcactga tgatacttgg cacgcgtcag tccacccctt ggttcctgct gttttccttg
    11941 ctggccatga tctcttgggg gttggacagc acgtttgcca aagctgccct gcccattccc
    12001 aagcacacta aagaggtctt cttcctgatc aatgtctgcg gcaccgcggc catcctctat
    12061 ttcgtcatgc gttttttcga gtcgcagaag gagcggctga tgcgggaagt ggacgagaaa
    12121 aaccgcttgc tggaacagga gcaggaa
//
LOCUS       DAOMFA010000035        11998 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_520_length_11998_cov_5.597505, whole genome shotgun sequence.
ACCESSION   DAOMFA010000035 DAOMFA010000000
VERSION     DAOMFA010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 11998)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11998)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11998
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_520_length_11998_cov_5.597505"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..651)
                     /locus_tag="PKW44_03090"
     CDS             complement(<1..651)
                     /locus_tag="PKW44_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HQR50606.1"
                     /translation="MATTKQTLMAIALFLCAATAHAGLPTQHWQSASGAQVYFVENHD
                     LPILDLSVGFAAGSSHDVAEKAGVAALTQSLLSLGAGGLSDDRISNRFADLGAQLGGD
                     FDADRAGLKLRTLSSERERSGALQLFASLLQQPDFPAAVLEREKARVIAGLREAATQP
                     ESITAKAFGKALYGNHPYALDEKVEPETAEKIGRDDLAASHRAHYTRSNALAAIIGD"
     gene            complement(638..1909)
                     /locus_tag="PKW44_03095"
     CDS             complement(638..1909)
                     /locus_tag="PKW44_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HQR50607.1"
                     /translation="MKIVVQEDHRSPVVVSQVWYRAGSIDEFNGTTGVAHVLEHMMFK
                     GTRKVPAGQFSRIVAAAGGRENAFTSRDYTVYFQQLEKSRLELSMKLEADRMANLNMS
                     DAEFAKEIQVVMEERRWRTDDKPQAQVGEQLMSATFQTHPYGRPVIGWMNDLEHMKAQ
                     DARDWYKRWYAPNNATLVVVGDVDPKQVFRLAQKYFGPLKAQKLPERKPQAEPEQRGE
                     RRLVVKAPAKLPYLAMAYHAPSLRDTKNDWEPYALEILAGVLDGNAASRLNRDLVRRD
                     RIAVDVSVGYDAVNRGPALFEFDGTPSEGTSVQDLEIAIRGQIEQLKQDGISEEELQR
                     VKAQVIAADVYQRDSMFYQAMQIGMLEGTGLSWRDLDDYPRRLQAVTAEQVQAAARKY
                     LVDDTLTVAVLDPQPIDENAPKHKEVPHGHH"
     gene            2138..3391
                     /locus_tag="PKW44_03100"
     CDS             2138..3391
                     /locus_tag="PKW44_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HQR50608.1"
                     /translation="MSLPYELFVGLRYTRAKRRNHFISFISMTSMVGIALGVAALIVV
                     LSVMNGFQEELRSRILGVASHMQVSGMDGTLEQWPAAAGLVSQHPEVQASAPYIMAQG
                     MLSYGQAVRGAIVRGVLPEREDEVADIGAHIHAGDLKALRPGEFGIVLGADLAQGLGV
                     GLGDKVVVMAPQGQFTPTGIVPRLKQFKVVGIFQIGMYEYDAGLALINIEDAAKLYRM
                     GDKVSGLRLKLTDLYRAPQVAAELSQQLGVHGNYAISDWTQQHTNFFRAVQMEKRVMF
                     VILTLIVAVAAFNIVSTLVMAVTDKRADIAILRTLGASPRSIMKIFIVQGALIGVIGT
                     LLGVVGGVLLALNIETVVPFIEQVFGIQFLAKDVYYISELPSHLEWADVVVITSVSLG
                     LSLLATLYPSWRASRINPAEALRYE"
     gene            3384..4073
                     /gene="lolD"
                     /locus_tag="PKW44_03105"
     CDS             3384..4073
                     /gene="lolD"
                     /locus_tag="PKW44_03105"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HQR50609.1"
                     /translation="MNDLLIGETILSCRDLHKTYAGLEVAVLNGIDLDIARSEQVAIV
                     GASGSGKSTLLHLLGGLDTPTQGEVSLLGQPLAKISEARRGELRNHALGFIYQFHHLL
                     PEFTALENVAMPLLIRRLAREQALQKAAEILGRVGLGKRMQHMPGELSGGERQRAAVA
                     RALVTNPQCVLADEPTGNLDRHTAHGVFDLMLELNRDLGTSFVVVTHDLELAGRIGRV
                     LRLEDGRLLPD"
     gene            4077..6473
                     /locus_tag="PKW44_03110"
     CDS             4077..6473
                     /locus_tag="PKW44_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HQR50610.1"
                     /translation="MRSAILAFVLGVWGLQQMPQLPALLWAILLPALLLPAWFLGRFP
                     LLAAQLARRMLFLFACIGTGFFWAAGMAQWRLSDALPPAWEGQDIQVVGVVASLPQLQ
                     ERGERFLLDVERVDTPHAKVPRRVSITRYFAGFREAPPDTVQGEFHAGERWRLTLRLK
                     RSHGSYNPYGFDFEAWALERNIRATGYVHNDPDNVRLEALVWQPAYLLERLREHIRER
                     FRQVLGDARYGGVLLALAIGDEGAIQAQDWQVFLRTGVNHLMSISGLHVTMVAALAFG
                     LVYALWRRMEWLTLRLPARKAAALAGMLVAGAYALLAGFAVPTQRTFYMLAVIAAALW
                     SGRVVSISLVLCWALLAVILLDPWAVLAPGFWLSFGAVALLVYAGSGRVARPHWLREA
                     AHTQWVVTLGLTPLLLALFQQVSVISPLANAFAIPLISLAVTPLALLGAVVPLDAILL
                     AAHGVMAACMALLEACARLPVAVWQQHAPSVWAVLLAMSGIAWMLLPRGFPLRWLGAA
                     VLAPMFLLLPAPPLPGELRVAVLDVGQGLAVVAQTARHALLYDTGPRYSEEADSGNRI
                     ILPYLRGSGIGRLDGLIVSHDDNDHSGGTLSVVGTMPVGWVASSLPESHPLRAAFVHH
                     VTCHAGQAWNWDGVRFEMLHPTLESYGIKKLKDNNRSCVLKISSANGSLLLPGDIERA
                     AEAELLERMPEALAADLLVAAHHGSKSSSTPEFIASVNPRVTVFTAGYRNRFGHPKSE
                     VVERYRSLGSILFRSDADGALLVDFTAGREMAVRAWRQVAPRYWQNVGNSPAGVDDAL
                     "
     gene            7042..7647
                     /locus_tag="PKW44_03115"
     CDS             7042..7647
                     /locus_tag="PKW44_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HQR50611.1"
                     /translation="MWSIIQAAGWPIWPLIFASVVSLAIIAERFYSLRASAVAPKNLL
                     PEVQAWLKQGALTKDACVRLEGHSPLGEIFASALCNVNTSREVMKESIEESGRAVTHK
                     LERYLTTLGTIATVSPLLGLLGTVIGMVELFGAFTATGHDVAQFARGISVALYNTAMG
                     IIVAVPSMIFYRHFRSTVDSLIVEMEQEAIKLVEIIQGERK"
     gene            7649..8071
                     /locus_tag="PKW44_03120"
     CDS             7649..8071
                     /locus_tag="PKW44_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HQR50612.1"
                     /translation="MNFQRGRRHEELEVNLVPLIDVLLVIIIFLVVSATFSRINELQI
                     NLPTADASAPQEKPLMITVGVDVSGHYVINDTPLAGSSVEAISAALRKAAGSGKEPTI
                     VINADASSTHQSVVNVMEAARQVGLTHITFATQVKSGS"
     gene            8097..9833
                     /gene="msbA"
                     /locus_tag="PKW44_03125"
     CDS             8097..9833
                     /gene="msbA"
                     /locus_tag="PKW44_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005319 - lipid transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006869 - lipid transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A export permease/ATP-binding protein
                     MsbA"
                     /protein_id="HQR50613.1"
                     /translation="MPETSTSLLSAKAVYLRLIDYAWRHWRMFVLGLLALATLSATNT
                     AFLAVIKMVTDEGFVQKNPQKLDILPWLLFGLLALRAFCGFFSDFSMRWVARRVVERI
                     RLDAFQRLMAFPVAYFDANSVGVITSKLTYNADQVSGAATRVVVSAVRDSLTIVGMLG
                     YMLYLNWHLTMVFLVMAPLMAWYLRKMTPRIRAAGRSAQESVGGMTRILEEAVSGQRV
                     VKIFGGEDYENRRFAEQASLHRRMQLRLARMAGLNSMVVELLASAGLAWVVYYSVGKF
                     SAGEFAAFVGAMLMLIAPIKSLTKLNEEIQVGVAAAQSVFGLMDTVTEEDAGSKVVEK
                     ARGEIEFRHVNLRYENAKRNALHEVNFRVSPGEKVALVGRSGGGKSSLVSLLPRFYEL
                     QQGVILLDGVDTRALTLKSLREQFALVSQDVTLFNDTVFNNIAYGSLRNLTESEVIAA
                     AKAAYAWEFIQQLPDGLYSEIGDRGVRLSGGQRQRIAIARAILKDAPILLLDEATSAL
                     DTESEKHVQAALDSLMQGRTTIIIAHRLSTIENADRILVMEKGEIVETGSHADLLALG
                     GHYAKLYHKQFH"
     gene            9834..10811
                     /gene="lpxK"
                     /locus_tag="PKW44_03130"
     CDS             9834..10811
                     /gene="lpxK"
                     /locus_tag="PKW44_03130"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019899386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HQR50614.1"
                     /translation="MQSWLERQWYLIGPWHVLLLPLSMLFFLIVSVRRLLYRRGLLKV
                     GKLPVPVIVVGNISVGGTGKTPLVIWLVEYLRQHGYRPGVISRGYGGTANQPMSVSMA
                     TDPFLAGDEPVLIARRTGCPVWVGKNRVETGRALLRVRPDCDVLVCDDGLQHYALARD
                     VEIAVVDGTRGFGNEFLLPAGPLREPLNRLLRVDAVVNNGGAGSAESVSMVLQGNLFR
                     NLSDPARSAVAEDFSRQHLHAVAGIGNPERFFAQLEQMGLLVERHAFPDHHPYRPDDL
                     RFAGTQAILMTEKDAVKCAAFAQPNWWYLQVDAKIDEALGLNVLQQLRK"
     gene            10813..10992
                     /locus_tag="PKW44_03135"
     CDS             10813..10992
                     /locus_tag="PKW44_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HQR50615.1"
                     /translation="MDAKLLEILVCPACKGPLVYKRPEQELVCRPCRLAYAIKDGIPV
                     MLEDEARQLPAEEEV"
     gene            11002..11775
                     /gene="kdsB"
                     /locus_tag="PKW44_03140"
     CDS             11002..11775
                     /gene="kdsB"
                     /locus_tag="PKW44_03140"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HQR50616.1"
                     /translation="MSAFKVVIPARHASTRLPGKPLLDIGGKPMVVRVAEKAVASGAA
                     EVCVATDHEAIAEAVRQHGFSAVMTRIDHASGTDRIAEVAAKRGWLGDEIIVNVQGDE
                     PLISPQLIREVALSLAEHDEAAIATACHPVHDRDSMFNPNIVKVVSDKLGYALYFSRA
                     PIPYARDAFASGAEVPAEMPVFRHVGIYAYRAAFLKAYSQLPPAEIEICEALEQLRAL
                     WHGYRISVALTGNAPETGVDTVADLERVRAIFSANPGYC"
     gene            11835..11911
                     /locus_tag="PKW44_03145"
     tRNA            11835..11911
                     /locus_tag="PKW44_03145"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11869..11871,aa:Met,seq:cat)
BASE COUNT         2079 a         3498 c         3895 g         2526 t
ORIGIN      
        1 gtcgccgatg atggcggcca gcgcgttgct gcgcgtgtaa tgggcgcggt gggaggcggc
       61 caggtcgtcg cggccgattt tctccgcggt ttccggctcc accttctcgt ccagcgcata
      121 agggtggttg ccgtacagcg ccttgccgaa cgccttggcg gtaatgcttt cgggctgagt
      181 tgccgcttcg cgcaggccgg cgatgacgcg cgccttctcc cgctccagta cggcggcggg
      241 gaaatccggc tgttgcagca gggaagcgaa cagttgcagc gcgccgctgc gctcgcgttc
      301 gctggacagg gtacgcagct tgaggccggc acggtcagcg tcgaaatcgc cgcccagttg
      361 cgcgccgagg tcggcaaaac ggttggagat ccggtcgtcg ctcaggccgc ccgcccccaa
      421 tgacagcagg ctctgggtca atgccgccac cccggccttt tccgccacgt cgtggctgct
      481 gccggcagcg aagccgacgc tgaggtcgag gatgggcagg tcgtggttct cgacgaaata
      541 cacctgcgct ccggaggcgc tctgccagtg ctgggtgggc aggccggcat gggccgttgc
      601 cgcacacagg aagagtgcga tggccattaa tgtctgctta gtggtggcca tgcggcacct
      661 ccttgtgctt gggggcgttt tcgtcgatcg gttgcgggtc gagcaccgcc acggtcagcg
      721 tgtcgtccac caggtacttg cgcgccgccg cctgcacctg ctcggcggtg accgcctgca
      781 ggcggcgcgg gtagtcgtcg agatcgcgcc aggaaagacc ggtgccttcc agcatgccga
      841 tctgcatcgc ctggtagaac atggagtcgc gctggtaaac atcggcagcg atgacctgcg
      901 ccttgacacg ctgtaactcc tcctcgctga tgccgtcctg cttgagttgt tcgatctggc
      961 cgcggatggc gatttcgaga tcctgtaccg atgtgccttc gctgggcgtg ccatcgaact
     1021 cgaacagcgc cgggccacga ttgaccgcgt cgtagccgac gctgacgtcc accgcgatgc
     1081 ggtcgcgccg caccaggtcg cggttcaggc gcgaggcggc gttgccgtcc agcacgccgg
     1141 ccaggatttc cagcgcgtaa ggctcccagt cattcttcgt gtcgcgcagc gagggcgcgt
     1201 ggtaggccat cgccaggtag ggcagcttgg ccggcgcctt gacgaccaga cggcgctcgc
     1261 ccctctgctc cggctcggcc tgcggcttgc gttccggcag tttctgggct ttcagcgggc
     1321 cgaagtattt ttgcgccagg cggaacacct gcttgggatc cacgtcgccc accaccacca
     1381 gagtagcgtt gttgggcgcg taccagcgct tgtaccagtc gcgggcatcc tgtgccttca
     1441 tgtgctcgag gtcgttcatc cagccgatca ccgggcggcc gtagggatgg gtctggaagg
     1501 ttgcggacat cagctgctcg ccgacctgcg cctgcggctt gtcgtcggtg cgccagcggc
     1561 gctcctccat caccacctgg atttccttgg cgaattcggc gtccgacatg ttgaggttgg
     1621 ccatgcggtc ggcttccagt ttcatcgaca gttccaaccg tgatttctcc agctgctgga
     1681 agtagacggt gtagtcgcgg ctggtgaagg cgttctccct gccgccggca gcggcgacga
     1741 tgcgcgagaa ctggccagcc gggacttttc tggtgccctt gaacatcatg tgttccagca
     1801 cgtgggcgac gccggtggtg ccgttgaact cgtcgatgct gccggcgcga taccacacct
     1861 gcgatacgac caccggcgag cgatggtctt cctgcacgac gattttcatg ccgttggtca
     1921 gcttgaattc atgggtgttt ccggccagcg ccgagatcgg cgcaagagcc agcaataaac
     1981 agagaagtcg tttcaccacg agttaatgct ccgtgaaatt tcggtggggg attatacaac
     2041 ccgaacttaa tatgcccggg caaaactgcc gatatgaccc ggcgtgtcgt ggttaagttc
     2101 gatgcgtgcc cggggtattc gagatagaat tgcgcccatg tccctgcctt acgaactctt
     2161 cgtcggcctg cgctataccc gcgccaaacg tcgcaaccac ttcatttcat tcatttccat
     2221 gaccagcatg gtcggtatcg cgctgggcgt ggcggcactg atcgtggtgc tgtcggtgat
     2281 gaacggtttc caggaggagt tgcgttcgcg catcctgggc gtggcctcgc acatgcaggt
     2341 cagcggcatg gacggcacgc tggagcagtg gccggcggcg gcagggctgg tcagccagca
     2401 tccggaagtg caggccagcg ccccttacat catggcccag ggcatgctgt cgtatggtca
     2461 ggcggtgcgc ggtgccatcg tgcgcggggt gctgccggag cgcgaggacg aggtggcgga
     2521 catcggcgcg cacatccatg ccggcgatct caaggcgctc aggcccggcg agttcggtat
     2581 cgtgctcggc gccgacctgg cacaggggct gggcgtgggg ctgggcgaca aggtggtggt
     2641 gatggcgccg caagggcagt tcacgccgac cggcatcgta ccccgcctca agcagttcaa
     2701 ggtggtcggc atcttccaga tcggcatgta cgaatacgat gccgggctgg cgctgatcaa
     2761 tatcgaagat gccgccaagt tgtaccgcat gggcgacaag gtttccggcc tgcggctgaa
     2821 gctgaccgac ctctatcgcg caccgcaggt ggcggccgag ctgtcgcagc aactgggtgt
     2881 gcacggcaat tacgctatca gcgactggac ccagcagcac accaatttct tccgcgcggt
     2941 acagatggaa aagcgcgtca tgttcgtcat cctgaccctg atcgtggcgg tggcggcgtt
     3001 caacatcgtc tccacgctgg tgatggcggt caccgacaag cgtgccgaca tcgccatcct
     3061 gcgcacgctg ggggccagtc cgcgcagcat catgaagatt tttatcgtgc agggggcact
     3121 gatcggtgtc atcggtaccc tgctgggcgt cgttggcggc gtgctgctgg cgctcaatat
     3181 tgaaaccgtg gtgccgttca tcgagcaggt gttcggcatc cagttcctgg ccaaggatgt
     3241 ctattacatc agcgaattgc cttcccacct ggaatgggcg gatgtggtcg tgatcacctc
     3301 ggtttcactg gggctgagcc tgctcgccac gctttacccg agttggcgcg cctccaggat
     3361 caacccggcg gaggcgctgc gctatgaatg atctgctgat aggggaaacc atcctctcct
     3421 gccgcgacct gcacaagacc tatgccgggc tggaagtcgc cgtgctgaac ggtatcgacc
     3481 tcgacatcgc ccgcagcgag caggtggcta tcgtcggcgc ctcaggctcc ggcaagagta
     3541 cgctgctgca cctgctgggc gggctggata cgcccacgca gggcgaggtg agcctgctcg
     3601 gccagccgct ggcgaagatc agcgaagccc gccgtggtga gctgcgcaac catgcgctcg
     3661 gcttcatcta ccagtttcac cacctgctgc cggaattcac cgcgctggaa aacgtcgcca
     3721 tgccgctgct gatccgccgc ctggcgcgcg aacaggcgtt gcagaaggcg gcggagattc
     3781 tgggcagggt gggcttaggc aagcgtatgc agcacatgcc gggcgagctt tccggcggcg
     3841 agcgccagcg cgcagcggtg gcgcgggcgc tggtgaccaa tccccaatgc gtgctggccg
     3901 acgagcccac cggcaacctg gaccgccaca ccgcgcacgg ggtgttcgac ctgatgctgg
     3961 aactcaaccg tgatctcggc accagcttcg tggtggtgac gcacgatctg gaactggcag
     4021 ggcgtatcgg gcgcgtgctg cggctggagg atggccgcct gctgccggac tgatccatgc
     4081 gcagcgccat cctggcgttt gtcctcggcg tctggggtct gcagcagatg ccgcaactgc
     4141 ctgccctgtt gtgggcgatt ttactgcctg ccctgttatt gcccgcctgg tttcttggga
     4201 gatttccact tctcgctgcg caactggcgc gacgtatgct gtttttgttt gcctgcatcg
     4261 gtaccggttt tttctgggca gccggcatgg cgcagtggcg gctgtccgat gcgctgccgc
     4321 cagcgtggga ggggcaggac atccaggtgg tcggtgtggt ggcctcgctg ccgcagctgc
     4381 aggagcgcgg tgagcgcttc cttctggacg tggagcgggt ggacacaccg catgccaagg
     4441 tgccgcgccg cgtttcgatc acccgttatt tcgccggttt tcgcgaagcg cccccggata
     4501 cggtgcaagg cgagttccat gccggcgaac gctggcggct gacgctgcga ctgaaacgat
     4561 cacatggtag ctacaacccg tacggcttcg atttcgaggc ttgggcgctg gagcgcaaca
     4621 tccgcgctac cggctatgtg cacaacgatc cggacaacgt tcggctggag gcactggtgt
     4681 ggcagccggc ttacctgctc gaacgcctgc gcgagcatat ccgcgagcgt ttccgccagg
     4741 tgctgggcga cgcacgctat ggtggcgtat tgctggcgct ggcgatcggc gacgagggcg
     4801 cgatccaggc gcaggactgg caggtgttcc tgcgtactgg cgtcaaccac ctgatgagca
     4861 tttccggcct gcatgtgacc atggtggcgg cgctggcgtt cggcctggtc tacgcgctat
     4921 ggcggcgcat ggaatggctg acgctgcgcc tgccggcgcg caaggcggcc gcactggcgg
     4981 gcatgctggt ggccggtgcc tacgccctgc tggccggctt cgccgtgccg acgcagcgga
     5041 ccttctacat gctggcggtg atcgccgccg cgctgtggtc ggggcgggtg gtttcgatct
     5101 cgctggtgct gtgctgggcg ctgctggcgg tgatcctgct cgacccgtgg gcggtgctgg
     5161 cgcccggttt ctggctgtcg ttcggtgcgg tggcgttgct ggtttatgcc ggtagcggcc
     5221 gcgttgccag gccgcactgg ctgcgcgagg cggcgcacac gcaatgggtg gtcacgcttg
     5281 gcctgacgcc cctgctgctg gcgctgtttc agcaggtgtc cgtgatttcg ccgctggcca
     5341 atgccttcgc cattccattg atcagtctgg cggtgacgcc gctggccttg ctgggggcgg
     5401 tcgttccgct ggatgccatc ctgctcgccg cgcacggcgt catggccgcc tgtatggcat
     5461 tgctggaagc ctgcgcgcgt ttgcctgtgg cggtgtggca gcagcatgcg ccatcggtgt
     5521 gggcggtatt gctggcgatg tccggcatag cctggatgct gttgccgcgc ggctttccgc
     5581 tgcgctggct gggtgcggcc gtgctggcgc cgatgttcct gctgctgcct gcgccgccgc
     5641 tgccagggga gctgcgggtg gcggtactgg atgtcggcca gggactggcg gtggtggcgc
     5701 agaccgcgcg tcatgccttg ctctacgaca ccgggccacg ctattcggag gaggctgaca
     5761 gcggcaaccg catcattctg ccttatctgc gcggctcggg tatcggccgg ttggatggcc
     5821 tgatcgtcag ccacgacgac aacgatcata gcggcggcac gctgtccgtg gtcggcacca
     5881 tgccggtggg gtgggtggct tcctcgctgc cggagtcgca cccgctgcgc gcggccttcg
     5941 tacaccatgt gacgtgccat gccggtcagg cgtggaactg ggacggggtg cgcttcgaga
     6001 tgctgcaccc cacactggag agctacggaa tcaagaagct gaaggacaac aaccgcagtt
     6061 gcgtgctcaa gatcagcagc gccaacggca gcctgctgtt accgggagac atcgagcgcg
     6121 ccgccgaagc cgagttgctg gaacggatgc cggaggcact ggcggccgac ctgctggtgg
     6181 cggcgcacca cggcagcaag tcttcttcca cgccggagtt tatcgccagc gttaatccga
     6241 gagtcaccgt attcaccgca ggttaccgca accgcttcgg ccatcccaag tcggaggtgg
     6301 tggagcgcta ccgaagtctc ggcagcatcc tctttcgcag cgatgctgac ggcgcgctgc
     6361 tggtggattt cacggcaggg cgggagatgg cggtgcgcgc ctggcgccag gtggcgccgc
     6421 gctactggca aaatgttggc aactcgcctg ccggcgtgga cgatgccctg tgacggtgaa
     6481 cggttctgct gccggaactc atcgccaata ttttctcccc gctgttaaca tgcttgctgg
     6541 ctttagctgc tacgaccacg acttctgggg cccttcaata catgctattc cgcttgttgt
     6601 tgatcttgat ggtgagcctg ccgcaaccac aaggccaatg tgctgctggc cggcatgcgc
     6661 attccaccca ggtgcggtcc ggcgtatacc acggaatttc tggagagcta cccgtttggc
     6721 gaagcagttc aagctgccgc tgctgtaatt tctgtttgac ggcgtggccg gaaaaggcga
     6781 gttgattcag gatgatggac tacacccgac cgcagccgca cagccgcgga ttctcgacaa
     6841 cgtctggacg gactgcgacc actgctggga aacccggagc agacaacccg gccgtaatga
     6901 acccggataa tctataagtt ggcacgccgc tttcaggcga gtgccggtgg caagttcgcg
     6961 aacagattgt ggctgcaggc ttgctgaaaa cggcaccgca ggataaagta gcgcgaattt
     7021 ttattgttaa ggaaattcat cgtgtggagc attattcagg ctgccggctg gcccatctgg
     7081 ccactgattt tcgcctcggt cgtatcgctg gcgattattg ccgaacgttt ttacagcttg
     7141 cgcgccagcg cggttgcacc caagaacctg ctgcctgaag tacaagcttg gctgaagcag
     7201 ggggctttga ccaaggacgc ctgcgtgcgc ctggaaggac attccccgct gggcgagatt
     7261 ttcgccagcg ccctgtgcaa cgtaaatact tcacgtgaag tgatgaagga gtcgatcgag
     7321 gaaagcggac gtgccgtgac gcacaagctg gagcgctact tgaccacgct tggcaccatt
     7381 gccaccgtca gcccgctgct gggcttgctg gggaccgtga ttggcatggt ggaactgttc
     7441 ggcgccttca ctgccaccgg ccatgacgtg gcgcagttcg cgcgcggcat ctcagtggcg
     7501 ctatacaaca ccgccatggg gatcattgtc gctgtgccca gcatgatttt ctaccgccat
     7561 ttccgttcca ctgtggacag cctgatcgtg gaaatggagc aggaagccat caagctggtg
     7621 gaaattatcc agggcgagcg caaataacat gaatttccag cgcggccgtc ggcacgagga
     7681 actggaagtc aatctggttc cgctgattga cgtattgctc gtcatcatta ttttcctggt
     7741 ggtcagcgcc actttttcgc gcattaacga actgcagatc aacctgccga cggccgatgc
     7801 cagcgcgccg caggaaaaac cgctgatgat cacggtcgga gtcgacgttt ccggccacta
     7861 cgtgatcaac gataccccgc tggccggcag tagcgtagag gcgatttctg cggcgttgcg
     7921 caaggcggcg ggtagcggca aggagccgac catcgtcatc aatgccgatg ccagcagcac
     7981 ccatcaatcg gtggtgaacg tgatggaagc cgcgcgccag gtcggcctca cgcacatcac
     8041 ctttgccaca caggtcaagt ccggttcttg atgacgctgt cggcgacaat gcgctgatgc
     8101 cagaaacctc tacttcctta ctgagcgcca aagcagttta tttgcgcttg atcgactatg
     8161 cctggcggca ttggcggatg tttgtgctcg gcctgctggc cctggccact ctttcggcca
     8221 ccaataccgc atttctggcg gtgatcaaga tggtgaccga cgaagggttt gtgcagaaaa
     8281 atccgcaaaa gctggacatc ctgccatggc tactgttcgg attgctggcg ctacgggcat
     8341 tttgcgggtt cttttccgat ttcagcatgc gctgggtggc gcgccgggtg gtggagcgta
     8401 ttcggctgga tgcgtttcag cgactgatgg cgtttcctgt ggcatatttc gatgccaact
     8461 ccgtcggcgt catcacctcc aagcttacct acaatgccga tcaggtttca ggcgcggcca
     8521 cccgggtagt ggtgagtgcc gtacgtgaca gcctgactat cgtcggcatg ctgggctata
     8581 tgctctacct caattggcac ctgactatgg tttttctcgt gatggcgccg ctgatggcct
     8641 ggtatctgcg caaaatgacg ccgcgcatcc gcgccgccgg gcggtctgcg caggagtcgg
     8701 tgggggggat gacgcgtatc cttgaggaag cggtgagcgg tcagcgcgtg gtcaagattt
     8761 ttggtggcga agattacgaa aaccggcgat ttgccgaaca ggcgagcctt caccgccgca
     8821 tgcagctccg cctcgcccgc atggccggcc tcaacagcat ggtggtggag ttgctggctt
     8881 cggcggggct ggcttgggtg gtctattatt cggtcggaaa attcagtgcc ggtgaatttg
     8941 ccgcgttcgt tggcgcgatg ctgatgctga ttgcaccaat caagagtctg accaagctga
     9001 acgaagaaat ccaggtaggg gtggcagcgg cgcaaagcgt atttggcctg atggatacgg
     9061 tgacagaaga ggatgccggc agcaaggttg tggaaaaagc acgcggcgag atcgaattcc
     9121 gtcatgtcaa cctgcgctac gagaacgcca agcgtaacgc tctgcacgag gtaaatttca
     9181 gggttagccc tggcgaaaag gtggcgctgg ttggccgctc gggcggtggc aaaagttcgc
     9241 tggtcagcct gttgccgcgc ttctatgaac tgcagcaggg cgtgatcctg ctcgatggag
     9301 tagatacgcg cgcattgacc ttgaagagcc tgcgtgaaca atttgctctg gttagccagg
     9361 acgttacgtt gttcaacgac acggtattta acaacatcgc ttacggcagc ttgcgcaatc
     9421 tgactgaatc ggaagtcatt gcggcagcga aagctgccta cgcctgggag ttcatccaac
     9481 aactgcctga cgggctttac agcgagatcg gtgatcgtgg cgtacggctt tccggcggcc
     9541 agcgccagcg tattgccatc gcgcgcgcca ttctcaagga cgcccccatc ctgctgctgg
     9601 acgaggcgac ttcggcgctg gataccgaat ccgaaaagca cgtgcaggcc gcacttgaca
     9661 gcctgatgca gggccgtacc accatcatca tcgcccaccg tctgtccacc attgaaaacg
     9721 ccgaccgcat cctggtgatg gaaaagggcg aaatcgtgga aaccggctcc catgccgatt
     9781 tgttggcatt aggtgggcat tacgccaagc tctaccacaa acagttccat taaatgcaat
     9841 cctggctgga gcggcagtgg tacctcatcg gtccttggca tgtgttgcta ctgccactat
     9901 ccatgctgtt tttcctgatc gtttcggtac gtcgcctgct ttaccggcgc gggctgttga
     9961 aggtaggaaa attgccggtg ccagtgatcg tggtcggcaa tatcagtgta ggcggcaccg
    10021 gcaagacccc tctggtgatc tggctggtcg agtacttgcg gcaacatggc tatcgccccg
    10081 gcgtgatcag tcgcggttat ggcgggactg ccaatcagcc catgtctgtc agtatggcga
    10141 cggatccctt cctcgccggc gatgagccgg tgttgatcgc ccggcgcact ggctgcccgg
    10201 tctgggtagg aaaaaatcgg gtcgaaaccg gtcgcgcgtt gctacgggtg cgtccggact
    10261 gcgatgtgct ggtttgcgat gatggtcttc agcattacgc cttggcgcgc gatgtcgaaa
    10321 tcgccgtggt cgatggaact cgaggcttcg gcaacgaatt tctgctgccg gcggggccgt
    10381 tgcgcgagcc gctaaaccgg ctgttgcggg tggacgccgt ggtgaacaac ggtggtgcgg
    10441 ggtctgctga aagcgtatcc atggtgttgc agggcaatct gttccgcaac ctcagcgatc
    10501 ctgcccgtag tgccgttgcc gaagattttt caaggcagca tctgcatgca gtagcgggaa
    10561 tcggcaaccc tgagcgcttc ttcgcacaac tagagcaaat gggcttgctg gtggagcggc
    10621 atgcttttcc cgatcaccat ccctatcggc ccgatgattt gcggttcgca ggtacgcaag
    10681 cgatactgat gaccgagaag gacgcagtca aatgcgccgc ttttgcccag ccaaactggt
    10741 ggtatctgca agttgatgcc aaaattgacg aggcgctggg cctgaacgta ctacaacagt
    10801 tgaggaaatg atatggatgc aaaactgctg gaaatcctgg tatgcccggc ttgcaaaggg
    10861 ccgttggtct acaagaggcc cgagcaggag ctggtctgcc gcccatgccg tctcgcttat
    10921 gccattaaag acggcatacc ggtaatgctg gaagacgagg ctcgtcagtt gcctgccgaa
    10981 gaagaagtct agggtgtcgt tgtgagtgcg ttcaaagttg tcattcccgc tcgccatgcg
    11041 tctacgcggc tgcccggtaa gcctttgcta gacattggcg gcaagccgat ggtggtgcgc
    11101 gtggcggaaa aggctgttgc cagtggcgcc gcggaagtct gcgtggcgac ggatcacgaa
    11161 gccattgccg aagccgtcag acagcatgga tttagcgctg tcatgacgcg cattgaccat
    11221 gcttccggaa ctgatcgtat cgcggaggtt gcagccaaac gtggttggct tggggatgaa
    11281 attatcgtca atgtgcaggg agacgagccg ctgatctcgc cgcaattgat tcgtgaagtg
    11341 gcgctgagtc ttgccgagca cgacgaagct gctattgcga cagcctgcca cccggttcat
    11401 gatcgcgact ccatgttcaa tcccaatatt gtgaaagtcg ttagcgacaa gctcggctac
    11461 gcactgtatt ttagtcgcgc gccgattccc tatgcgcgtg atgcttttgc ctcgggagcc
    11521 gaggtgcctg cagaaatgcc ggtattccgc cacgttggca tctatgccta ccgtgccgcc
    11581 tttctcaagg cctattccca actgccgccg gcggagatag agatttgcga agccttggag
    11641 caattgcgcg ccctttggca cggctatagg attagcgttg ccttaaccgg caatgcaccg
    11701 gagaccgggg tggatactgt ggccgatcta gagcgcgtgc gcgcaatctt ctcggcaaac
    11761 cccggatatt gttgacacag ggttggtgga tttgctatag ttccacctct cggcggcacc
    11821 aacgtcgaat cgggcgcggg gtggagcagt ctggcagctc gtcgggctca taacccgaag
    11881 gtcacaggtt caaatcctgt ccccgcaacc agtcaatttt ctagttgacc aagattttca
    11941 gcgctgtata atgcgcggct ctcgttactt tggtagcggg gtttgaagta actgctct
//
LOCUS       DAOMFA010000036        11821 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_529_length_11821_cov_5.274435, whole genome shotgun sequence.
ACCESSION   DAOMFA010000036 DAOMFA010000000
VERSION     DAOMFA010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 11821)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11821)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11821
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_529_length_11821_cov_5.274435"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1692)
                     /locus_tag="PKW44_03150"
     CDS             complement(<1..1692)
                     /locus_tag="PKW44_03150"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HQR50617.1"
                     /translation="MALEIKRNEENNKIWSHLRQDWYNETPEERVRQEYLCVLVNEYG
                     FALEQIGEEIPVTERGCGQARADFLIWRSPAAKTGKETPLIVVECKADNVTIDARTYT
                     QGANYAQYSKARFFVTHNNRETKYWKVDHAKMMPNFDEIENIPHADASDKQIEELIAK
                     LKTFKEDEFADLLHKCHNIIRNREHLDPAAAFDEIAKILFVKTGVERKLKQGRQRLNL
                     FTAEFLDQQKVVLDDPINALFNQTKAEFKADRIFADDDKINLKHPTVVAIVKELERYN
                     LSDTSEDIKGIAFERFLGRTFRGEIGQFFTPRPIVEFMVRMLQPQEGEIVCDPASGSG
                     GFLIRVFELVRQQILADADRQYQAFKAEIEANTGMDEAAKARALSERFAAIQSTLDQS
                     REGSRMWQLSNRSIYGTDANDRMARTSKMNMIMHGDGHGGVHHHNGFLNVNGIFEGRF
                     DIILTNPPFGANVEPTDIVQEAEVALAPEIERLYLERYGNQYREASARLQAAAKAQQA
                     IAALFKLPRSAAAKIKTEILFIERCLDLLKTGGRLGIVLPEDVFKNQSLDYVRHLV"
     gene            complement(1716..2600)
                     /gene="dinD"
                     /locus_tag="PKW44_03155"
     CDS             complement(1716..2600)
                     /gene="dinD"
                     /locus_tag="PKW44_03155"
                     /inference="COORDINATES: protein motif:HMM:NF008573.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HQR50618.1"
                     /translation="MVKTDLPTVGGQSFEALKLANEHGAEYWSARDLQPLLGYTQWRR
                     FEDAIKRAMTSCEQSGNRPDYHFAGAGKMIELGKGGAREVEDYHLSRFACYLIAQNGD
                     PRKPEIAQAQKYFAVQARKQELTEQLAADQERLELRKQTSEEFKALSGAARQAGVQDR
                     MFGVFHDAGYKGLYGGLGGEAIKKKKGIPEKEQLLDRMNATELAANQFRMTQTRDKLA
                     RENVNDQQRAIRAHEQVGKEVRTAIERIGGTLPEQIPPAEHIKEVEKRIKSTVPKMQL
                     DEKDAGGLLGGNDQQSDK"
     gene            complement(2621..3725)
                     /gene="prfB"
                     /locus_tag="PKW44_03160"
     CDS             complement(join(2621..3643,3645..3725))
                     /gene="prfB"
                     /locus_tag="PKW44_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832626.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HQR50619.1"
                     /translation="MEAERLNAIANHLQDMETRTVDLRRYLDFDGKKDRLTEVSRLAE
                     DPAIWNDAERAQALGREKKGLEAVVLTLEHIASALRDAGELFEMARAEDDDDTLIAIE
                     ADAQGLEKEVAALEFRRMFSNPMDPNNCFVEFQSGSGGTEAQDWAGMLLRMYLRYCEK
                     KGFQVEVLEETEGDIAGIKGASIKVSGDYAYGTLRTESGVHRLVRKSPFDSNAKRHTS
                     FASVQIFPEVDDSIEVDINPADLRIDTYRASGAGGQHINKTDSAVRITHLPTNTVVQC
                     QNDRSQHRNKAEAMAMLKAQLYALELRKRNEEKQALEDAKTDIGWGHQIRSYVLDQSR
                     IKDLRTNVEIGNTQGVLDGDLDPFISASLKQGV"
     gene            complement(3765..5027)
                     /locus_tag="PKW44_03165"
     CDS             complement(3765..5027)
                     /locus_tag="PKW44_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HQR50620.1"
                     /translation="MDIGSLFTSENGLEYVPAFATSLAVGLLIGVERERSPAAKAGLR
                     TFSLVSLLGTMLAFLSDRSGSSWPLVAGLVVVGAMIIAAYVGDDQDKDPGTTTQAALI
                     LSFGLGAIIWYGYSTLAVMAAIVMTAMLYYKPELEGMSRRLSRADVASMLQFAVLSFI
                     ILPILPNQDYGPYDTLNPYQTWLMVVLISGISLAGYVALRLVGQRYGAPLLGFLGGLV
                     SSTATTLVYARHGRNSAMLNLAVVVITIANLVVLVRLAVLGGVLAPAILPHLLPVLVM
                     GFVSGAGVTFYWWRRMQRGGDLPIPEIKNPTEIKTALSFGALYAVVLLAAAWLSDVAG
                     SRGLYLLALASGMTDVDAISLSSLRLFGQGKLLAEQTVLAITIAYLSNLAFKFGLLAF
                     FGGKALAYRAAPSVGAVALGVLAGALLL"
     gene            complement(5163..6290)
                     /locus_tag="PKW44_03170"
     CDS             complement(5163..6290)
                     /locus_tag="PKW44_03170"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HQR50621.1"
                     /translation="MILPPSMLGMLGGGQLGRFFVIAAHEMGYKVTVLDPDKHSPAGM
                     IADVHLCAAYDDAAALDKMARTCAAVTTEFENVPASTLDALAKSVLVYPPASAVAVCQ
                     HRVSEKNFLRDNGFPHAPYAEINSEADIPAAAGGLYPGILKVARFGYDGKGQARVSSP
                     EEALAAFRSFKGEQCVLEKMLALDYEVSVVLARDTAGNVQAFPTTENAHRDGILDISI
                     SPARGSAGVREQAQILARGVAEKLAYVGTLGVEFFVCNDSLTINEMAPRPHNSGHYTI
                     DACVTNQFEQQVRALCGLPLGDPRLHSTAVMVNLLGDIWRDGEPEWQALFAQADLKLH
                     LYGKPEARPGRKMGHFTVIGDKADAVLAAAMAARQRIRVEG"
     gene            complement(6287..6787)
                     /gene="purE"
                     /locus_tag="PKW44_03175"
     CDS             complement(6287..6787)
                     /gene="purE"
                     /locus_tag="PKW44_03175"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020183865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HQR50622.1"
                     /translation="MTDKQPLVAIIMGSDSDWPVMKAAAAILDDFGVAYEARVVSAHR
                     TPDLMFEFAENAAARGYRAIIAGAGGAAHLPGMVAAKTTLPVLGVPVPSKHLQGQDSL
                     LSIVQMPRGIPVATFAIGEAGAANAGLFAVAQLATTDKALADKLAAFRRQQSEKVLAM
                     TLKGDA"
     gene            complement(6802..7920)
                     /gene="proB"
                     /locus_tag="PKW44_03180"
     CDS             complement(6802..7920)
                     /gene="proB"
                     /locus_tag="PKW44_03180"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HQR50623.1"
                     /translation="MKSLLANAKTIVVKVGSSLVTNEGRGLDQAAIAAWADQVARLRH
                     HGRKVVLVSSGAVAEGMQRLGWSKRPTAVNELQAAAAVGQMGLVQMYESCFARHKLHT
                     AQVLLTHDDLADRKRYLNARSTLRTLLDLNAIPIINENDTVVTDEIRFGDNDTLGSLV
                     ANLIEADALVILTDQQGLYSADPRKDASATLISQATAGDPGLEKMAGGAGSSVGTGGM
                     LTKVLAAKRAARSGAHTIIASGREQDVLVRLLEGEVIGSHLRAPEMKTAARKQWLADH
                     LQVRGRLALDAGAIRALKEDGKSLLPIGVTAVSGEFGRGELVVCVDTHGYEVARGLVN
                     YNAVETARILKKPSSEIEQILGYVDEPELIHRDNLVIL"
     gene            complement(7917..8954)
                     /gene="obgE"
                     /locus_tag="PKW44_03185"
     CDS             complement(7917..8954)
                     /gene="obgE"
                     /locus_tag="PKW44_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HQR50624.1"
                     /translation="MKFIDEATIKVYAGDGGNGVATFRREKYEPMGGPSGGDGGRGGS
                     IHVVADRNINTLVDYRYTRSFRAQRGENGRSSDCYGAKGEDMVLRVPVGTVVTDKGTG
                     NVMADLAQDGQQALLAKGGNGGLGNIHFKSSINRSPRQCTKGEPGEEFELYLELKVLA
                     DVGLLGMPNAGKSTFIRAVSAAKPKVADYPFTTLYPNLGVVRVDTNRSFVIADIPGLI
                     EGAAEGAGLGHQFLRHLARTRLLLHIVDIAPFDDAIDPVAEARAIVEELRKYDEGLYN
                     KPRWLVLNKVDMLPEDERDARRVAFLREFGWDAPSFCISALSGEGCQKLTYAIMDYLD
                     ANKLEPEAITE"
     gene            complement(9144..9401)
                     /gene="rpmA"
                     /locus_tag="PKW44_03190"
     CDS             complement(9144..9401)
                     /gene="rpmA"
                     /locus_tag="PKW44_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HQR50625.1"
                     /translation="MAHKKAGGSTRNCRDSNAQRLGVKAFGDQLVSAGSIIVRQRGTK
                     MYPGDNVGMGKDHTLFAKVEGKVAFAVKGPLRHKFVSIVPV"
     gene            complement(9414..9728)
                     /gene="rplU"
                     /locus_tag="PKW44_03195"
     CDS             complement(9414..9728)
                     /gene="rplU"
                     /locus_tag="PKW44_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HQR50626.1"
                     /translation="MYAVIKTGGKQYRVAAGDKLKVEQLTGEVGSELVIDKVLMLADG
                     DKVTIGSPLINGASVKATVVSHGRHDKVMIFKMRRRKHYRKTQGHRQNYTEIQIQQIA
                     AK"
     gene            9859..10827
                     /gene="ispB"
                     /locus_tag="PKW44_03200"
     CDS             9859..10827
                     /gene="ispB"
                     /locus_tag="PKW44_03200"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HQR50627.1"
                     /translation="MPLASIQDRIAADMSAVDAVIRKRLHSEVALVSQVAEYIVNSGG
                     KRLRPALVLLAAGIFGKVMPQHHELAAVVEFIHTATLLHDDVVDGSSMRRGKATANTL
                     FGNAASVLVGDFVYSRAFQMMVSIENMRVMEVLADATNIIAEGEVLQLLNCHDADVTD
                     EAYLKVIHYKTAKLFEAATRLGAIISGASERDEAALAEYGMRLGTAFQLIDDVLDFSG
                     DSSEIGKNLGDDLAEGKPTLPLLYAMRHGHEQQQQVIRHAIEHGGLKDLALVMQAVHA
                     TGALDHVRKLAANEAESGCRAISHLPDSKYKQALVELAAFAVSRNF"
     gene            10839..10915
                     /locus_tag="PKW44_03205"
     tRNA            10839..10915
                     /locus_tag="PKW44_03205"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10873..10875,aa:Pro,seq:cgg)
     gene            complement(11001..11762)
                     /locus_tag="PKW44_03210"
     CDS             complement(11001..11762)
                     /locus_tag="PKW44_03210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR50628.1"
                     /translation="MSPLGISCIALVCVFGSALLGLYLQARQPDHHLSADSKDAVKLA
                     TGIIATMAALLLGLLISSAKSSFDQINNELVGSAANVVVLDRTLADYGPETGEIRDSL
                     KVYYKTRVNLETSGKASEVAKLDSAKMVHELEDIRAKVLHLSPGNDAQRELQNEALHT
                     MGEMVHARWLLMLQKDNSVSIPLVALLVAWLSIIFAAFGLFSPCNKLVVTKLFLSALS
                     VSAAIFLILELNGPLTGLVTVSKASLYDAIAHLGQ"
BASE COUNT         2253 a         3582 c         3513 g         2473 t
ORIGIN      
        1 gaccaaatgg cgcacgtagt ccaaagattg gtttttgaat acgtcttcgg gcaacactat
       61 ccctaagcgc ccgcccgtct tgagcaggtc aagacaacgc tcgataaaca gaatttcggt
      121 cttgattttg gcggctgcgc tcctgggcag cttgaacagg gcggcaatgg cctgttgcgc
      181 cttggcggcg gcttgcaggc gggccgaggc ttcacggtac tggttgccgt agcgttccag
      241 gtagaggcgt tcgatttcgg gggcgagcgc gacttcggct tcctgcacga tgtcggtcgg
      301 ttcgacattg gcgccaaacg gcgggttggt caggatgatg tcgaagcgtc cctcgaaaat
      361 gccgttgacg ttgagaaagc cattgtggtg atgcacgccg ccgtggccgt cgccgtgcat
      421 gatcatgttc atcttgctgg tgcgcgccat gcggtcgttg gcgtcggtgc cgtaaatgga
      481 gcggttggaa agctgccaca tgcgcgagcc ttcgcgcgat tgatccagcg tggactgaat
      541 ggcggcaaac ctttcggaga gcgcgcgggc tttggcggct tcatccatgc cggtattggc
      601 ttcgatttca gccttgaatg cctgatattg gcggtcggca tcggcgagga tttgctggcg
      661 caccagttca aacacgcgaa tcagaaagcc accggagccg ctggcggggt cgcagacaat
      721 ctcgccttcc tgcggttgca acatgcgcac catgaattcg acaatcgggc gcggggtgaa
      781 aaactggccg atttcaccgc ggaaggttct ccccagaaaa cgctcgaagg cgatgccctt
      841 gatatcctcg ctggtgtcgg aaaggttgta gcgctccaac tccttgacga tggcgaccac
      901 ggtgggatgc ttaagattga tcttgtcgtc gtcggcaaaa atgcggtcgg ccttgaactc
      961 ggccttggtc tggttgaaca aggcgttgat agggtcatcc aacacgactt tttgctgatc
     1021 caggaattcc gccgtaaaca ggttgaggcg ctgacgacct tgcttgagct tgcgttccac
     1081 gccggttttg acgaacagga ttttggcgat ttcgtcgaag gcggcggcgg ggtcgaggtg
     1141 ttcgcggttg cggatgatgt tgtggcactt gtgcagcagg tcggcgaact cgtcttcctt
     1201 gaaggtcttg agcttggcga tcaattcctc gatctgtttg tcgctggcgt cggcgtgggg
     1261 gatgttctcg atctcgtcga agttgggcat catcttcgcg tgatcgacct tccagtattt
     1321 ggtctcgcgg ttgttgtggg tgacgaaaaa acgcgccttg ctgtattggg cgtagttggc
     1381 gccctgggtg taagtgcggg cgtcaatcgt gacgttgtcg gccttgcatt cgaccacgat
     1441 gagcggcgtt tccttgccgg tcttggctgc cgggctacgc cagatcagga agtcagcgcg
     1501 cgcttggccg caaccgcgct cggtcacggg gatttcctcg ccaatctgct ccagcgcaaa
     1561 gccgtactcg ttgaccagta cgcagaggta ttcctggcgc acccgctctt ccggtgtttc
     1621 gttgtaccaa tcctggcgca ggtggctcca gattttgttg ttttcctcgt tgcgcttgat
     1681 ttcgagtgcc atgtgaatac cctttgtgcc catgcctatt tgtctgattg ttgatcgttc
     1741 ccaccaagca agccaccggc atctttttca tccaactgca ttttcggcac ggtagatttg
     1801 atgcgctttt ccacttcctt gatatgttcg gcgggcggaa tctgctccgg cagtgtgccg
     1861 ccaatgcgct cgatggcggt gcggacttcc ttgccgactt gttcgtgggc gcggatggcg
     1921 cgctgctggt cgttgacgtt ttcgcgcgcc agtttgtcgc gggtttgggt catgcggaac
     1981 tggttggccg ccagttcggt ggcgttcata cggtcgagca actgctcttt ttcgggaatg
     2041 ccttttttct tcttgatcgc ttcgccacca agcccgccgt ataagccttt gtagcctgca
     2101 tcgtggaaca cgccgaacat ccgatcttgc acgcccgctt gccgcgccgc gccggagagc
     2161 gccttgaatt cctcggaagt ttgcttgcgc agttccagcc gctcctggtc ggcggccagt
     2221 tgttcggtga gttcctgctt gcgggcctga accgcgaagt atttttgcgc ctgggcaatc
     2281 tccggcttgc gcgggtcgcc gttctgggca atcaggtagc aggcaaaacg ggagaggtgg
     2341 tagtcttcga cttcgcgtgc gccccctttg ccgagttcga tcattttgcc ggcgccggca
     2401 aaatgataat cggggcggtt tcccgattgt tcacaggagg tcatcgcgcg cttgatggcg
     2461 tcctcgaaac ggcgccactg ggtgtagccc aatagcggct gcaagtcgcg cgcgctccag
     2521 tattcggcac cgtgttcgtt ggccagtttc aacgcctcga atgattgccc gcctaccgtt
     2581 ggtaagtcag tttttaccat gccagccctc ctgtaaagaa tcaaactccc tgcttcaaac
     2641 ttgcactgat aaacgggtcc agatcgccat ccagcacgcc ttgggtgttg ccgatttcga
     2701 cattggtgcg cagatccttg atgcgcgact ggtcgagtac gtaggagcga atctggtggc
     2761 cccagccgat gtcggtcttg gcatcttcca gcgcctgctt ttcctcgttg cgcttgcgca
     2821 gttcgagcgc atacagctgc gccttcagca tggccatggc ctcggccttg ttgcggtgct
     2881 gcgagcggtc gttctggcat tgcaccaccg tattggtggg gagatgggtg atgcgtacgg
     2941 cggagtcggt cttgttgatg tgctggccgc cggcgccgga ggcgcgatag gtgtcgatgc
     3001 gcaggtcggc ggggttgatg tcgacctcga tggaatcgtc cacttccggg aaaatctgca
     3061 cgctggcaaa gctggtgtga cgtttggcgt tggagtcgaa aggcgattta cgtaccaggc
     3121 gatgcactcc gctttcggta cgcaaggtgc cataggcgta gtcgcccgag accttgatgg
     3181 aggcgccctt gatcccggca atatcgccct ctgtttcttc cagcacttcg acctgaaagc
     3241 ccttcttctc gcaatagcgc aggtacatgc ggagcagcat gcctgcccag tcctgcgcct
     3301 cggtgccgcc gctgccggac tgaaactcga caaagcagtt gttgggatcc atcgggttgg
     3361 agaacatgcg gcggaattca agcgcggcaa cctccttttc cagcccctgg gcatcggcct
     3421 cgatagcgat caaggtgtcg tcgtcgtcct cggcgcgtgc catctcgaac aactcgccgg
     3481 cgtccctgag ggcggaggca atgtgctcca gcgtcagcac cacggcctcc aggcccttct
     3541 tttcgcggcc cagcgcctgg gcgcgttcgg catcattcca gatggccgga tcttcggcca
     3601 ggcggctgac ttcggtcagg cgatcctttt ttccgtcgaa gtcaaagata cctccgcaag
     3661 tcgacggtac gggtttccat gtcctgcagg tgattggcaa tggcgttgag gcgttcggct
     3721 tccatgttgc ttccagtcga attcaaagct gggattatag cagttcagag cagcaaggcg
     3781 ccggccagca cccccagcgc taccgcgccc acgctcggcg cggcacggta tgccagggcc
     3841 ttgccgccga agaaggccag caggccgaac ttgaacgcca ggttggacag ataggcgatg
     3901 gtgatggcaa gcaccgtctg ctccgccagc agcttgccct ggccgaacag gcgcaggctg
     3961 gatagcgaaa tggcgtcgac atcggtcata ccggaagcca gcgccagcag gtaaaggccg
     4021 cggctgccgg cgacatccga cagccaggcc gccgccagca gcaccacggc atacaacgcg
     4081 ccaaaggaaa gcgcggtctt gatctcggtg ggattcttga tttccgggat cggcaaatca
     4141 cccccgcgtt gcatgcggcg ccaccagtag aaggtcaccc cggcgccgga gacgaaaccc
     4201 atcaccagca ccggcagcaa gtgcggcaga atggccggcg ccagcacgcc gcccagcacc
     4261 gccaatcgta ccagcaccac caggttggcg atggtgatga ccaccaccgc cagattgagc
     4321 atggcactgt tcctgccatg gcgcgcgtag accagcgtgg ttgcggtact cgacaccaga
     4381 ccgccgagga aacccagcaa cggtgcgcca tagcgctgcc cgaccagccg cagggcgaca
     4441 tagccggcca ggctgatgcc ggatatcagc accaccatca gccaggtctg gtaagggttc
     4501 agcgtgtcgt acgggccata atcctggttc ggcaggatgg gcaggataat gaaggaaagc
     4561 accgcgaact ggagcatgga ggccacatcg gcgcgcgaca ggcggcggct catgccctcc
     4621 agttccggct tgtaatacag catggcggtc atgacgatgg ccgccatgac ggccagggtg
     4681 ctgtaaccgt accagatgat cgcacccagg ccaaaactca gtatcaatgc ggcctgggtc
     4741 gtggtgccgg gatccttgtc ctggtcatcg ccgacgtagg cggcaataat catggcgccg
     4801 acgaccacca gcccggccac cagcggccag gacgacccgc tgcgatccga cagaaacgcc
     4861 agcatggtac ccaacagcga taccagactg aatgtacgca gccccgcctt ggcagccggg
     4921 ctgcgctcgc gctccacgcc gatcagcagg cccaccgcca gactggtggc gaacgccggg
     4981 acatattcca ggccgttttc actggtaaac aggctaccga tatccatggt ggcaaggttc
     5041 tccgctgggc aatagcttca tggtacccgt ttgaccgccc ttggcaactc cttgccaagg
     5101 cgcgagacga gttcaccccg gaaacaaaaa agccaaccgt aaggctggct ttccgcatgg
     5161 actcagccct ctacccggat gcgctggcgt gcagccatcg ccgctgccag cacggcgtcg
     5221 gccttgtcac cgatgacggt gaagtgtccc atcttgcggc cggggcgtgc ctcgggcttg
     5281 ccgtaaagat gcagcttgag atcggcctgt gcgaacagtg cctgccattc cggttcgcca
     5341 tcgcgccaga tgtcgcccag caggttgacc atcaccgccg tactgtgcag gcgcgggtca
     5401 cccaaaggca ggccacacaa tgcacgtact tgctgctcga actggttggt gacgcaggca
     5461 tcgatggtgt agtggccaga attgtggggg cgcggcgcca tttcgttgat cgtcaggctg
     5521 tcgttgcaga cgaagaattc cacccccagc gtgccgacgt aggccagttt ctccgccacg
     5581 ccgcgcgcca ggatctgggc ctgctcgcgc accccggcgc taccgcgcgc cggtgagatg
     5641 ctgatgtcga gaataccgtc gcgatgggcg ttttcggtgg tggggaacgc ctgcacgttg
     5701 ccggcagtat cgcgcgccag caccacggag acttcgtagt ccagcgccag cattttctcg
     5761 agcacgcatt gctcgccctt gaagctgcga aacgctgcca gcgcttcttc tggcgagctc
     5821 acccgcgcct ggcctttgcc gtcatagcca aagcgcgcca ctttcagaat gcccggatac
     5881 aagccgccag cggcagccgg gatatcagct tcgctgttga tctcggcata tggggcgtgg
     5941 ggaaagccgt tgtcgcgcag gaaattcttt tcgctgacgc ggtgctgaca aaccgccacc
     6001 gcggaggctg gcggatatac cagtactgat ttagccaagg catccaatgt gctggccggc
     6061 acgttctcga actcggtggt caccgcagcg caggtgcgcg ccatcttgtc cagtgcggcc
     6121 gcatcatcgt aggcggcgca cagatgcacg tcggcgatca tgcccgccgg gctgtgtttg
     6181 tctggatcga gcaccgtgac cttgtagccc atttcgtgcg cggcgatgac gaagaagcgg
     6241 ccgagctggc cgccgccgag catgcccagc atggacggcg gcagaatcat gcatccccct
     6301 tcagggtcat ggccagcact ttctcgctct gctgcctgcg gaaggcggcg agcttgtccg
     6361 ccagcgcttt gtcggtcgtc gccagctgcg ccacggcgaa cagtccggcg ttggcggcgc
     6421 cagcttcgcc aatggcaaag gtagcaacgg gaatgcctct tggcatctgc acgatggaca
     6481 gcagggaatc ctgcccctgc agatgcttgg aaggtaccgg cacacccagt accggcaagg
     6541 tcgtcttggc cgccaccatg cccggcaggt gtgcggcacc gccggcaccg gcgatgatgg
     6601 cacggtagcc gcgcgcggcg gcattctcgg caaactcgaa catcagatcc ggggtgcggt
     6661 gcgccgacac tacgcgcgct tcatacgcca caccgaagtc gtcgaggatg gcggcggcgg
     6721 ccttcatcac cggccagtcg ctgtcggagc ccatgatgat ggcgaccaaa ggttgtttgt
     6781 cagtcatgat tttcccttag tttagagaat taccaggttg tcccggtgga tcagttccgg
     6841 ctcgtccacg taacccagga tctgctcgat ctcgctgctg ggcttcttca gaatgcgcgc
     6901 cgtttccacc gcattgtaat tgaccaggcc gcgtgcaacc tcatatccgt gcgtatcaac
     6961 gcagaccacc agttcgccgc gcccaaattc gccggaaacc gccgtcaccc cgatcggcag
     7021 caggcttttg ccgtcttctt tcagcgccct gatagcaccg gcatcaagcg ccagcctgcc
     7081 tcgcacctgc agatggtcag ccaaccactg ttttcttgcc gcagtcttca tttccggcgc
     7141 gcgcaggtgg ctgccgatga cttctccctc cagcaaccgc accaggacat cctgctcgcg
     7201 gccggaagcg atgatggtat gcgccccgct acgcgcggca cgcttggcgg ccagcacctt
     7261 ggtcagcatg ccgccggtac ccacgctgct gccggcgcca cctgccatct tttccagtcc
     7321 cggatcacct gcggtagcct gactgatcag cgtggcgctt gcatccttgc gcgggtcggc
     7381 ggagtagaga ccctgctggt cggtcaggat gaccagcgcg tcggcctcga tcaggttggc
     7441 caccagcgac cccagcgtat cattgtcgcc aaaacggatt tcgtcggtga ccacggtgtc
     7501 gttctcgttg atgatgggaa tggcgttgag gtcgagcagg gtgcgcagcg tgctgcgtgc
     7561 gttgaggtaa cgcttgcggt ccgccagatc gtcatgcgtc agcagcacct gcgccgtatg
     7621 aagcttgtgt ctggcgaagc aggattcgta catctgcacc aaacccatct ggccgacggc
     7681 cgccgccgcc tgcagttcgt tcacggctgt cggacgcttg ctccagccca agcgctgcat
     7741 gccttccgcc accgcaccgc tggacaccag cacgaccttg cggccatggt ggcgcaggcg
     7801 cgcgacctgg tcggcccagg cggcaatggc ggcctgatcc agcccgcggc cctcgttggt
     7861 gacgaggctg gagccgacct tgaccacgat ggttttggca ttggcgagca gcgatttcat
     7921 tcggttatgg cttccggttc cagcttgttg gcgtcgagat aatccatgat ggcgtaggtc
     7981 agcttctggc agccttcgcc gctcagggcg gagatacaga acgacggcgc gtcccagccg
     8041 aattcacgca gaaaggccac acgcctcgcg tcgcgttcgt cctccggcag catatcgacc
     8101 ttgttcagca ccaaccagcg cggcttattg tatagcccct cgtcgtattt cctcaattcc
     8161 tcgacgatgg cgcgcgcctc ggctacgggg tctatcgcgt cgtcgaacgg cgcgatgtcc
     8221 acgatatgca acagcaaccg ggtacgcgcc agatggcgca ggaactgatg gccgagacca
     8281 gcgccctctg ccgcgccctc gatcagacca ggaatgtcgg cgatgacaaa gctgcggttg
     8341 gtatctaccc gcaccacgcc cagattggga tacagcgtgg tgaagggata gtccgccacc
     8401 ttgggcttgg cagcggaaac ggcacggatg aaggtggact ttccggcatt gggcatgccg
     8461 agcaggccga cgtccgccag caccttcagt tccaggtaaa gctcgaattc ctcgcccggt
     8521 tcacccttgg tgcattggcg cggggagcgg ttgatgctcg acttgaagtg gatgttgccc
     8581 agaccaccgt tgccgccctt ggcgagcagc gcctgttgac catcctgcgc caggtcggcc
     8641 atgacgttgc cggttccctt gtcggtgacc acagtaccga cgggtacgcg cagcaccata
     8701 tcctcgccct tggcgccgta gcagtccgaa ctgcgtccgt tctcgccgcg ctgcgcgcga
     8761 aaactgcgcg tgtagcggta atccaccaag gtattgatat tgcggtcggc caccacgtgg
     8821 atggagccgc cacggccgcc gtcaccgccg cttggcccgc ccataggctc gtatttctca
     8881 cggcggaacg tggcaacgcc gttaccgccg tcgccggcat agaccttgat ggtggcttcg
     8941 tcgataaatt tcatggcaac aatcagcttt cagcgatgcg gcattccttg tcgcattcaa
     9001 acgaagacaa ggcggaggcg cgcagacagt atgaatagta cggcaagcgc cgaacaacgc
     9061 cgtattcgtt tgaatgcgac aaggaattaa aacaaaaaag ccctatcgtt cctgataggg
     9121 ctttttgctc gggcatgctt gcctcagacg ggaacgatgc tcacgaactt gtgacgcaac
     9181 ggacccttga ctgcaaaggc aaccttgcct tccaccttgg cgaacagggt gtgatccttg
     9241 cccatgccaa cgttgtcgcc ggggtacatc ttggtgccgc gctggcgcac gatgatgctg
     9301 ccggcggaaa ccagctggtc gccaaatgcc ttgacgccca gtcgttgtgc gtttgaatcg
     9361 cgacagttcc gggtactgcc gcctgctttt ttgtgtgcca tggctaactc cttttacttg
     9421 gcagcaatct gctgaatctg gatttcagtg taattctggc gatggccttg ggtcttgcga
     9481 tagtgcttgc ggcggcgcat cttgaaaatc atcaccttgt cgtgacggcc atgagaaacc
     9541 acggtcgcct tgacgcttgc gccgttgatc aacggggaac caatcgtcac cttgtcgccg
     9601 tccgccagca tcaacacctt gtcgatgacc agttcgctgc cgacttcgcc ggtcagttgt
     9661 tctaccttca gtttgtcgcc ggccgcaacg cgatattgct tgccgccagt cttgataacc
     9721 gcatacatga ttaagcctcg tttgccatgc atttcaaatg aaccgcgcat tatacggaac
     9781 acattcaaaa atgcaaagcc gattttacgg caccccctat ggtaaaatcc gcccactgtt
     9841 acattccatg ccatccaagt gcctctagcc tccatacaag accgcatcgc ggcagacatg
     9901 agcgcggtcg atgcggtgat ccgcaaacgc ctgcattccg aagtcgcgct ggtaagccag
     9961 gttgccgagt atatcgtcaa cagcggcggc aagcgcctac gtccggcgct ggtgctactc
    10021 gccgcaggca tcttcggcaa ggtcatgccg cagcaccacg agctggcagc cgtggtcgaa
    10081 ttcatccaca ccgccaccct gctgcacgac gacgtggtgg atggctcttc catgcggcgc
    10141 ggcaaggcga ccgccaatac cctgttcggc aacgccgcca gcgtgctggt gggcgatttt
    10201 gtctattcac gcgccttcca gatgatggtc agcattgaga acatgcgcgt gatggaggtg
    10261 ctggccgacg ccaccaacat catcgccgaa ggcgaagtgc tgcaactgct caactgccac
    10321 gacgccgacg tcaccgacga agcctactta aaggtgatcc attacaagac cgccaaactg
    10381 ttcgaagccg caacacggct tggcgccatc atctccggcg cttctgagcg ggacgaagct
    10441 gcgctggcgg aatacggcat gcgcctcggc acggcattcc agctgatcga tgacgtgctg
    10501 gatttcagcg gagacagcag cgaaatcggc aagaacctcg gcgacgacct ggccgaaggc
    10561 aagcccaccc tgcctttgct ctacgccatg cgccacggcc acgaacaaca acagcaagtc
    10621 atccgccacg ccatcgaaca cggcggcctg aaagacctgg cgctggtgat gcaggccgtc
    10681 catgccaccg gcgcgcttga ccacgtgcgc aagctcgccg caaatgaagc agaatcgggc
    10741 tgccgggcga tttctcacct ccccgattca aaatacaagc aagctctggt agaattggcg
    10801 gcctttgcgg tcagccgcaa tttctaagca agtcaaatcg gggtgtagct tagcctggta
    10861 gagcgctacg ttcgggacgt agaggccgga ggttcgaatc ctctcacccc gaccaagatc
    10921 ttccttcgaa ccaatctccc caacccttta gtaagggcca cttaagttca gccgttgaat
    10981 gtaacggagg gagcttccga ctactgccca aggtgcgcga tcgcgtcata aagggaggct
    11041 ttggagaccg tgaccagccc cgtgagcggc ccgttaagct ccagaatcag gaaaatcgcc
    11101 gctgaaaccg acagcgcact caaaaaaagc ttggtaacaa cgagtttgtt gcatggtgaa
    11161 aacaggccga atgcggcaaa aatgatcgac agccatgcca caagaagtgc taccagggga
    11221 atagaaacag aattgtcttt ttgcaacatc agcagccagc gtgcatgaac catttccccc
    11281 atggtatgca atgcctcatt ctggagttct cgctgggcat cgttgcctgg cgaaagatga
    11341 agcaccttgg cacggatgtc ttccagctcg tgaaccatct ttgcgctatc cagttttgcc
    11401 acttcggaag ccttgccaga agtctccaaa ttgacccggg tcttgtaata aactttgagg
    11461 gaatcgcgaa tttccccagt ctccggtcca tagtctgcca gcgtgcgatc aaggacaaca
    11521 acgttggcgg cgctgccgac aagttcgttg ttgatttggt cgaacgaact cttggccgag
    11581 gaaatcagca accccaggag caaggccgcc atggtggcga taataccggt agcaagcttt
    11641 accgcatcct tcgagtccgc actcaggtga tggtcgggct gcctggcttg caggtacaac
    11701 ccaagcaatg cgctaccaaa gacgcaaaca agggcaatac aggaaattcc aaggggactc
    11761 atgatgacaa cttacctcga tgcttaattt ctgtgcaatg cgcgatctta taattaattg
    11821 g
//
LOCUS       DAOMFA010000037        11784 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_534_length_11784_cov_5.393810, whole genome shotgun sequence.
ACCESSION   DAOMFA010000037 DAOMFA010000000
VERSION     DAOMFA010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 11784)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11784)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11784
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_534_length_11784_cov_5.393810"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..823)
                     /locus_tag="PKW44_03215"
     CDS             complement(<1..823)
                     /locus_tag="PKW44_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HQR50629.1"
                     /translation="MNDLSKAAVSLVTVDEGGEGQRIDNFLAKQLKGVPKSHLYRILR
                     SGEVRVNSKRVDATHRLALGEVVRIPPVRTAAAAPKPASPVTSKFDGKVLFEDDVLLV
                     IDKPAGIAVHGGSGISRGVIEQLRLERPQAKFLELVHRLDRETSGVLLLAKKRSTLVA
                     LHEAIRGDAMEKRYLILVCGEWKDKKRRVRLPLKKYTLESGERRVSVDEEGQSSETIF
                     HLRQSFGEFTLLEAELITGRTHQLRVQLAHLGFPIAGDDKYGDFELNKTLQKRGLK"
     gene            1311..3773
                     /locus_tag="PKW44_03220"
     CDS             1311..3773
                     /locus_tag="PKW44_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HQR50630.1"
                     /translation="MKRMLFNATQEEELRVAIVDGQRLIDLDIEHAGKEQRKSNIYKG
                     VITRIEPSLEAAFVNYGTDRHGFLPFKEVARSYFQGDADGRSSIKEALREGQELIVQV
                     DKDERGNKGAALTTFISLAGRYLVLMPNNPRGGGVSRRIEGEDRNELRDVMAQLEVPS
                     GMSIIARTAGIGRSLEELQWDLNYLMQLWTAVEGASTLQKGAFLIYQEGSLVIRAIRD
                     YFQPDIGEILIDTPEIHEQALQFMNHVMPGNVSRVKLYRDEVPLFSRFQIEHQIETAF
                     AREVRLPSGGAIVIDHTEALVSIDVNSGRATRGSDIEETAFKTNLEAAEEAARQLRLR
                     DLGGLVVIDFIDMESQRNQREVENRLRDALHYDRARVQTGKISRFGLMELSRQRLRPS
                     LGETNHIPCPRCSGTGHIRGIESTALHILRITQEEAMKENSAVIQVQLPVEAATFLLN
                     EKRAEIHTIEQRMGVKVVLIPNIHLETPNYKIVRLRHDDVSEDNVRASYELVELPSEI
                     EEAGAAPQKAETVKQVAAVKGITPSAPAPIPTAATEAQKASLFGRFRSWLGKISGSKE
                     ELVEEKPQPRRRENARRGERGGRNDKRGEPRREGRGEKREPLRDATAKPQEPRQPREP
                     RAERERPQRPPVPAPQAQTAATEAGEAREPRSRRGRRGGERRERPPRNEAQQRPLDLN
                     EETVQAATVEPLTVPVEVETAKSAIQAVAAEQVTKPTDAAPAGASRQPAEAPAAAKPE
                     RKPRPQRSRKPREIASAAPIVVPDLAESGLVMIETKADAVVVAPLAAETPLKRKAQPA
                     AWQKKTAEKVADEPLVMVETQK"
     gene            complement(3864..5897)
                     /gene="uvrB"
                     /locus_tag="PKW44_03225"
     CDS             complement(3864..5897)
                     /gene="uvrB"
                     /locus_tag="PKW44_03225"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HQR50631.1"
                     /translation="MIVTFPNSLYQLYQPFPPAGDQPVAIEKLVEGVRSGKRFQTLLG
                     VTGSGKTYTMANVIARLGRPAIVMAPNKTLAAQLYSEFREFFPNNAVEYFVSYYDYYQ
                     PEAYVPSRDLFIEKDSSINEHIEQMRLSATKALLEREDSIIVATVSAIYGIGDPVDYH
                     GMVLHLTQGEKISQRDVILRLTGMQYDRNEFDFARGTFRVRGDVVDVFPAENSETALR
                     ISLFDDEVETLTLFDPLTGQILHKVPRFTVYPSSHYVTPRETTLRAIEQIKAELRERT
                     DFYLKNHKLVEAQRIEQRTRYDLEMLNELGFCKGIENYSRHLSGRKVGEPPPTLIDYL
                     PKNALMIIDESHVTVPQIGAMYKGDRARKENLVDYGFRLPSALDNRPLRFEEFEQVMR
                     QCVFVSATPSEYEAAHQQQVVEQVVRPTGLIDPEIVVKPADTQVDDLLSEAKARVEAG
                     ERVLVTTLTKRMAEDLTDYLAEHGVKVRYLHSDIDTVERVEIIRDLRLGEFDVLVGIN
                     LLREGLDIPEVSLVAVLDADKEGFLRSERSLIQTTGRAARNLNGKVIFYANTITRSMK
                     LAMGETERRRKKQTAFNELHGITPIGVSKRIKDIIDGVYDKDGAQKELKAAQVMAGYE
                     HMSEKQLLKEMKRIEKGMHDAAKNLEFEKAAELRDSLKKLREQFYGAEPPDIA"
     gene            6011..7021
                     /locus_tag="PKW44_03230"
     CDS             6011..7021
                     /locus_tag="PKW44_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HQR50632.1"
                     /translation="MYRVLLFIFPLLIVQGVLAFDQQSLMKVFFSVVLVRGYDTSGGL
                     AYGTGVVVGENKVATNCHVLRKTTQAWISQAEDVYRITSVQVDARHDLCLLGTEAMPL
                     KPVPLGNTAEVGKGDEVLSLGHSNGVLSPMTSGGQVKSIYSYDHGNVIRTNARFSLGA
                     SGSPLFDSQGRLIGINTFKTPGRAAYFYAMPVEWLAKLEKMPLQTRLPVSGQAFWEFP
                     DEEKPFFMQIALPHLNEDWAKLLEISQRWTQAEPGSAEAWYELGTAQEGLGKPEEAQQ
                     SYSTATTINPLHSEALFRLGVFASKRGDRAKVHNVSLILAEIDSEMAAEFNKAAGCTA
                     EC"
     gene            7094..8287
                     /locus_tag="PKW44_03235"
     CDS             7094..8287
                     /locus_tag="PKW44_03235"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HQR50633.1"
                     /translation="MELSRRVQAIKESPTLAVTARAARLKAEGRDIIGLGAGEPDFDT
                     PQHIKDAAKKAIDSGFTKYTPVGGIPGLKKAIVGKFKRENGFDYSVNEVIVGVGGKQC
                     IFNLALAVLDAGDEVVIPAPYWVSYADIGIVAEAVPVIVECGIEQGFKLTPAQLEAAI
                     TPRTKLFFINSPSNPTGSVYTLEELKALGEVLKKHPRVLIATDDMYEHVNLTGAPFVN
                     ILNATPELKGRTIVLNGVSKAYSMTGWRIGYAAGPAHIIKAMEILQSQSTSNPTSISQ
                     VAAQAALEGDQGCIEPMVKAFRERHEYVVERFNQIPGLKCLKAGGAFYAFPDARVAIA
                     RLHAAGKIKEATDLALSDYLLESVGVAVVPGSAFGADGYFRVSFATSMDNLRNALDRI
                     AEALA"
     gene            complement(8342..8713)
                     /locus_tag="PKW44_03240"
     CDS             complement(8342..8713)
                     /locus_tag="PKW44_03240"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HQR50634.1"
                     /translation="MTEHAYTEEEIRSRLTAELPHWYYEDGWIRRKYKTAGWKATLMV
                     VNTVGHLAEAAWHHPDLTVSYAFVIVKLVTHTAKGVTDKDFALAKKIEEVICWQPGKV
                     GGALEGTPNDDVRFKYLKYDE"
     gene            complement(8734..9339)
                     /locus_tag="PKW44_03245"
     CDS             complement(8734..9339)
                     /locus_tag="PKW44_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019898174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridylate kinase"
                     /protein_id="HQR50635.1"
                     /translation="MWVVKLGGSLLGTPELRQWLDLLARQSDGRVVIVPGGGIFADTV
                     RSAQTVGGYDDVAAHHMALLGMEQYGLALQSLQPQLVTASSELEIAERSWQHRAIVWL
                     PSQMVLADDSIPMNWEVTSDSLAAWLARKIGANRLVLVKHVEFTRLQPPLRQLVEEGV
                     LDAAFTEFATGLACPIHVVDKSGYADFALALAGAPIPTLAF"
     gene            complement(9326..10693)
                     /gene="pabB"
                     /locus_tag="PKW44_03250"
     CDS             complement(9326..10693)
                     /gene="pabB"
                     /locus_tag="PKW44_03250"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008153 - para-aminobenzoic acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HQR50636.1"
                     /translation="MIAELPYRANGAELFGHIAQRPWAVYLDSGRPGSSYGRYDILAA
                     DPFITLVTRCGKTEIRQGEDVRISSEDPFMLLQRTLRQFPASAGELPFAGGAIGYFSY
                     DLARRLEHLPDTAVDAEHMPEMAVGVYDWAVVLDHEQRRAWLVGQGFDARTQQRWDEL
                     RALFSASAVTQAETFKTTGLISSNMDMDTYAAAFRRVLDYIQAGDCYQVNLAQRFSTK
                     AEGDAWTAYLQLRRISPAPFSAYLNTPAAQILSASPECFLKVRDGRVETKPIKGTRPR
                     SLDLAQDLENADELRASLKDRAENLMIVDLLRNDIGKSCAVGSVKAERLFELESFANV
                     HHLVSTVTGTLAEGRDAIDLLRGCFPGGSITGAPKLRAMQIIEELEPHRRGVYCGAIG
                     YIGFDGNMDTSIAIRTAVYSGGSIRFWAGGGIVADSEMDKEYRETWDKASSMLQLMEQ
                     FHVGG"
     gene            complement(10690..11739)
                     /locus_tag="PKW44_03255"
     CDS             complement(10690..11739)
                     /locus_tag="PKW44_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HQR50637.1"
                     /translation="MNNKLPLTLGWDLGGAHLKAALVDADGRALRVFQQPCPLWRGSA
                     HLEQAIAAVMAGLGDSPLRHTVTMTGELADIFPSRGEGVAHLTQLMADRLGDADLLIY
                     AGRSGLVATHAVLEHLADIASANWHASAAFLAARGAQGLFIDVGSTTADLVVLHHGQL
                     ATRGYSDAERMRYDELVYTGAIRTPVMAVAQQLPFSGEWQRLAAEHFATMADVYRLTG
                     ELPESYDMADTADGGEKTPQASARRLARMVGRDLQDAPMPQWRQLAFAVRSQQLHLLQ
                     FAVERTLSHALLDDEAPLIGAGAGRFLVRELASRLGRKYRDASEWVTAVPETAEWAGI
                     CLPAYAVAWLARNEA"
BASE COUNT         2491 a         3647 c         3351 g         2295 t
ORIGIN      
        1 gtttgaggcc acgcttttgc aacgtcttgt tcaactcgaa atcaccgtat ttgtcatcgc
       61 cagcaatggg gaagcccaga tgcgccagct gcacccgcaa ctggtgggtg cggccggtaa
      121 tcagttccgc ctccagcagg gtgaattcgc cgaacgactg acgcaggtgg aaaatagttt
      181 cggacgactg cccctcctca tccacactga cccgcctttc gccgctctcc agcgtgtact
      241 tctttagtgg cagcctgacc cgccgtttct tgtctttcca ctccccacac accaatatca
      301 gatagcgctt ttccatggca tcgccacgga tggcctcgtg caacgccacc agcgtgctgc
      361 gctttttggc caacaacagt acgccggagg tttcgcggtc gaggcggtgc accagctcca
      421 ggaatttggc ctgcgggcgc tccaggcgca gttgttcgat cacgccgcgg ctgatgccgc
      481 tgccgccgtg cacggcaatg ccggccggct tgtcgatcac cagcagaacg tcgtcctcaa
      541 acagcacctt gccgtcgaat ttcgatgtca ccgggctggc gggcttgggg gcggccgctg
      601 ccgtgcgcac tggcggaatg cgcaccacct cgcccaaggc cagccggtgc gtggcgtcca
      661 cgcgcttgct gttgacgcgc acttcgccgc tgcgcaggat gcgataaagg tggcttttcg
      721 gcaccccctt caactgtttg gcgagaaaat tgtcgatgcg ctgcccctcg ccgccctcat
      781 cgaccgtcac caggctcacc gctgctttgc ttaaatcgtt catctcacct atactgctga
      841 cttgcttgaa aaagcaatca agaattctgg ttaatgccgt tcaccacaga caacaagaaa
      901 cggctaaaaa tttctgaaat cagccttctc tccctgtgcg ggatacgtct gcactagcga
      961 cgagcggaag tttggattca gaacaaatgc gctcaaagct gccgcgcacg gaatggcaca
     1021 taattgccgc gcggcgggaa acgggattgt aaccgaaaag gctgttttgc cgggcatcaa
     1081 ccgcaccata tatataaagt tttacgttaa tttaaatatt taacgatttg atttaatgta
     1141 gtaaagacag gcatagctct ctagaaatac gagtgtgtta ttgcttggat ttgcttgcgt
     1201 atcgcggtag ttccgggctt ttgcctgttg tcgtcacccc ccttccctcc ggccgcctca
     1261 ttgcggccag ccatgcacgc ccgctttgaa gagcgcggga gaagaaccaa atgaaacgca
     1321 tgcttttcaa tgcgacgcag gaagaggagc tgcgcgtcgc cattgtcgat gggcaacgac
     1381 tgatcgatct cgatatcgag cacgccggca aagaacaacg caagagcaat atctacaagg
     1441 gtgttatcac ccgcatcgag ccttcgctcg aagccgcctt cgtcaattat ggcaccgacc
     1501 gccatggctt cctgccattt aaggaagtgg cacgcagcta cttccagggc gatgcagacg
     1561 gccgctccag catcaaggaa gccctgcgcg aaggccagga actgatcgtg caggtggaca
     1621 aggatgagcg cggcaacaaa ggcgcagccc tgaccacttt tatcagtctg gccggccgct
     1681 atctggtact gatgcccaac aacccgcgcg gcggtggcgt gtcgcgccgc atcgagggcg
     1741 aagaccgcaa cgaactgcgc gatgtcatgg cgcaacttga agtgccttcc ggcatgagca
     1801 tcattgcccg caccgccggc atcggccgct cgctggaaga gttgcagtgg gacctcaact
     1861 atctgatgca attgtggacc gccgtcgaag gcgcatccac cctgcagaaa ggcgccttcc
     1921 tgatctatca ggaaggcagc ttggtaatcc gcgccattcg cgactacttc cagcccgaca
     1981 tcggcgaaat cctgatcgac acaccggaaa tccacgagca ggcgctgcag ttcatgaacc
     2041 acgtaatgcc gggtaacgtc tcgcgcgtca agctgtaccg cgacgaagtc cccttgttct
     2101 cgcgcttcca gattgaacac cagatcgaaa ccgcattcgc ccgtgaagtg cggctgccct
     2161 ctggcggcgc catcgtgatc gatcataccg aggcgctggt ttctatcgac gtcaactccg
     2221 gccgcgccac acgtggtagc gacattgagg aaaccgcttt caagaccaat ctggaggccg
     2281 ctgaagaagc ggcccgccag ttgcgcctgc gcgatctcgg cgggttggtt gtgatcgact
     2341 tcatcgatat ggaaagccag cgcaaccagc gtgaagtgga aaaccgtttg cgcgatgccc
     2401 tgcattacga ccgcgcccgc gtccagactg gaaagatttc acgttttggc ttgatggagc
     2461 tgtcgcgcca gcgcctgcgc ccatccctgg gtgaaaccaa ccacatcccc tgtccacgtt
     2521 gtagcggcac cggccatatc cgaggcatcg aatccaccgc cctgcacatc ctgcgcatca
     2581 cccaggaaga ggcgatgaag gaaaacagcg cagtcatcca ggtgcaactc ccggtagaag
     2641 ctgccacctt cctgctcaac gaaaagcgcg cagagattca caccatcgaa cagcgcatgg
     2701 gcgtcaaggt cgtgctgatc cccaacatcc acctggaaac gccgaactac aagatcgtac
     2761 gcctgcgcca tgacgacgtc agtgaagaca acgtgcgcgc aagctacgaa ctggtggagc
     2821 tgccttcgga aatcgaggaa gccggcgccg ccccgcaaaa agctgaaacc gtgaaacagg
     2881 tggctgcggt caaaggtatc acaccttcag caccggcccc catccccacg gcggcaacgg
     2941 aagcccagaa ggcctcgctg ttcggccgtt tcaggagttg gcttggcaag atttccggtt
     3001 ccaaagagga actggtagaa gaaaagccgc agccgcgcag gcgtgaaaac gcccgccgtg
     3061 gtgagcgcgg cgggcgcaat gacaagcgcg gtgagccacg tcgcgaaggt cgtggcgaaa
     3121 agcgcgaacc cttgcgtgac gccaccgcca agccacagga accgcgccaa ccacgcgagc
     3181 cccgtgccga acgcgaaagg ccgcagcgcc ctcccgttcc tgctccacaa gcgcaaactg
     3241 cagcaacgga agccggagaa gccagagaac cacgcagtcg tcgcggccgc cgcggcggtg
     3301 agcgccgtga gcgccccccg cgcaatgagg cacagcaacg cccgctggat ctgaacgagg
     3361 aaactgttca ggcggcaaca gtcgaaccgt taacggtacc cgttgaagtt gaaaccgcca
     3421 aatcagccat acaagctgtt gcggccgaac aagtgaccaa accgaccgat gcagcaccag
     3481 ccggcgcgtc cagacaacca gctgaagctc cggccgcagc caagccggaa aggaagccgc
     3541 gcccgcaacg tagccgcaag ccaagggaaa tcgccagtgc cgcccccatc gtcgtacctg
     3601 acttggcgga aagcggcttg gtcatgattg agaccaaggc agatgccgtt gtagtggctc
     3661 cgctcgcggc ggaaacgccg ctcaagcgca aggcgcaacc cgctgcctgg caaaagaaga
     3721 ccgccgaaaa agtcgcagac gagccgctag tgatggtgga aacgcagaag taatcggaat
     3781 cgcccgaagc agttaaaagc cccgcttatg cggggctttt tttgtagtca atgtcgccaa
     3841 gcggtcttgc ctgaaccact ctgtcatgcg atgtccggtg gctccgcgcc gtaaaactgt
     3901 tcacgaagct ttttcaggct gtcgcgcagt tctgcggctt tctcgaattc caggtttttt
     3961 gcagcgtcgt gcatgccttt ttcgatgcgc ttcatttcct tcagcagctg cttttcgctc
     4021 atgtgctcgt agcccgccat gacctgcgcg gccttgagtt ccttctgcgc gccatccttg
     4081 tcatagacgc cgtcgatgat gtccttgatg cgcttgctga cgccgatagg ggtaataccg
     4141 tgcagttcgt tgaaggccgt ctgtttcttg cggcggcgtt cggtttcgcc catcgccagc
     4201 ttcatcgagc gggtgatggt gttggcgtag aaaatcacct tgccgttgag attacgtgcg
     4261 gcgcggccgg tggtctggat cagcgagcgc tcggagcgca gaaagccctc cttgtcagca
     4321 tccaggacgg ctaccagcga cacctccggg atgtccaggc cttcgcgcag caggttgatg
     4381 ccaaccagca cgtcaaattc gccgaggcgc aaatcgcgga tgatttccac ccgctccacg
     4441 gtgtcaatat cggagtgcag ataacgcacc ttgacgccat gctcagccag gtaatccgtc
     4501 agatcttccg ccatgcgctt ggtgagtgtg gtaaccagca cgcgctcgcc cgcctccacc
     4561 cgcgccttgg cttcggacaa caggtcatcc acttgggtat cagccggctt gaccacgatt
     4621 tccgggtcta tcagcccggt cggacgcacc acctgctcca ccacctgctg ctggtgtgcg
     4681 gcttcgtatt ccgacggcgt ggcggagacg aacacgcact ggcgcatcac ctgttcgaat
     4741 tcctcgaaac gcagcgggcg gttatccagc gccgaaggca ggcggaaacc gtaatccacc
     4801 aggttctcct tacgcgcccg gtcgcccttg tacatggcgc cgatctgcgg aactgtcaca
     4861 tgagattcgt cgataatcat cagtgcgttc ttcggcaggt agtcgatcaa cgtcggcggc
     4921 ggctcgccaa ccttgcgacc ggacagatgg cgtgagtagt tttcgatgcc cttgcagaaa
     4981 cccagctcgt tgagcatttc cagatcgtag cgggtgcgct gctcgatgcg ctgtgcttcc
     5041 accagcttgt ggttcttgag gtagaaatcg gtacgttcgc gcagctccgc cttgatttgt
     5101 tcgatggcgc gcaaggtggt ttcgcgcggc gtcacgtaat ggctggaagg atagaccgtg
     5161 aagcgcggca ccttgtgcag aatctgtccg gtgagcgggt cgaacaaggt cagcgtttcc
     5221 acctcgtcgt caaacaatga aatccgcagt gcagtttccg agttttccgc cgggaacacg
     5281 tccaccacgt cgccacgcac tctaaatgta ccgcgcgcaa agtcgaattc gttgcggtcg
     5341 tactgcatgc cggtcagccg caggatcaca tcgcgttggc taattttttc gccttgggtg
     5401 aggtgtagca ccatgccgtg gtaatccacc ggatcaccga taccgtagat ggccgacacg
     5461 gtagcgacga tgatgctgtc ctcgcgctcc agcagcgcct tggtagcgga aagccgcatc
     5521 tgctcgatat gctcgttgat gctggaatcc ttctcgatga acaggtcacg cgacggcacg
     5581 taggcttcgg gctggtagta atcgtaatag gaaacgaaat actccactgc gttgttgggg
     5641 aagaactcgc ggaattctga ataaagttgc gccgccagcg tcttgttggg cgccatgacg
     5701 atggccggcc gccccaaacg ggcaataacg ttggccatgg tgtaggtctt gcctgaacca
     5761 gtgacaccaa gcaaggtctg gaagcgcttg cctgaacgca cgccctcgac cagcttttct
     5821 atggcaaccg gctggtcgcc ggcaggcggg aatggctgat acagttggta caagctattg
     5881 gggaatgtga cgatcacggc gtatcctgca aaaaccagcg gccattgtat caggcgcttt
     5941 tggcaacaag gagccggaaa cttttgcgcg ctgcaagagt cactggatag taaccaaggt
     6001 gaggattatc gtgtaccgtg tactgctgtt cattttcccg ctgctgatag tgcaaggtgt
     6061 cctcgccttc gatcagcaat cactgatgaa agtgttcttc tccgttgtcc tggtgcgcgg
     6121 ctacgatact agcggcggcc tggcctacgg taccggtgtc gtggtaggcg aaaacaaggt
     6181 ggccaccaac tgccacgtgc tgcgcaagac cacccaggcg tggatttccc aagccgagga
     6241 tgtctaccgg atcacatcag tgcaggtcga tgcccgccac gacctttgcc tgctcggcac
     6301 cgaagccatg ccattgaaac ccgttcctct cggcaatacg gcagaagtcg gcaagggtga
     6361 cgaagtccta tcgctaggac attccaatgg tgtattgagc ccgatgactt ccggcggcca
     6421 ggtaaaatcc atctactcct acgaccacgg caatgtcata cgcaccaacg ccagattttc
     6481 tctcggcgcc agcggcagcc cactgttcga tagtcagggc aggctgattg gcatcaacac
     6541 cttcaagaca ccgggaaggg ctgcctattt ctacgcgatg ccggtcgaat ggctggccaa
     6601 actggagaaa atgccactac aaaccaggct tcccgtctcc ggccaggcgt tctgggaatt
     6661 tccggatgag gaaaaacctt tcttcatgca gattgccctg ccacacctga atgaagactg
     6721 ggccaagctg ctggaaatca gccagcgctg gacccaggcc gagccgggga gcgcggaagc
     6781 ctggtacgaa ctcggcacgg cgcaggaagg tctgggaaag cctgaggaag cgcagcaatc
     6841 ctacagcacg gcaaccacca tcaaccctct acatagtgag gccttattca ggcttggtgt
     6901 gttcgcctcg aaacgcggcg accgcgccaa agtgcacaac gtcagtttga tcctggcaga
     6961 aattgatagt gaaatggcag cagagttcaa caaggcggcc ggttgcactg ccgagtgctg
     7021 aaagcaggtt tatccgaagc aggtttatcg gtaaaattgc acaaattttg ccgataattc
     7081 aaggacccag attttggaac tctcccgccg cgttcaagcc atcaaggaat ctcccaccct
     7141 cgccgtcacc gcccgcgctg cccgcctcaa ggccgaaggc cgcgacatca tcggtctcgg
     7201 cgccggcgaa ccggatttcg ataccccgca gcacatcaag gatgcggcca agaaggccat
     7261 cgacagcggc ttcaccaagt acaccccggt aggcggcatc cccggcctga agaaggccat
     7321 tgtcggcaag ttcaagcgag agaacggctt cgactacagc gtcaatgagg tcatcgttgg
     7381 cgtcggcggc aagcagtgta ttttcaatct ggcgctggca gtcctggatg ccggcgacga
     7441 agtggtgatt cctgcgccct actgggtatc ctacgccgat atcggcattg tcgcggaggc
     7501 agtgcccgtc atcgtggaat gcggcatcga gcagggcttc aagctcaccc cggcccaact
     7561 ggaggccgcc atcaccccgc gcaccaagct gttcttcatc aactcgccgt ccaacccgac
     7621 cggctcggtc tatacgctgg aagaactgaa ggcgctgggc gaagtgctga aaaagcatcc
     7681 gcgcgtattg atcgccactg acgacatgta tgaacacgtc aacctgaccg gcgcgccctt
     7741 cgtcaacatt ctcaacgcca cgccggaact gaaaggccgt acgattgttc ttaatggcgt
     7801 ctccaaggcc tactccatga ccggctggcg catcggctac gctgccggcc cggcgcacat
     7861 catcaaggcc atggaaatcc tgcagtccca aagcacctcc aaccccacct cgatctccca
     7921 ggtcgctgcg caggcggcgc tggaagggga ccagggctgc atagaaccca tggtcaaggc
     7981 gttccgcgag cgccacgaat acgtggtcga acgcttcaac cagattcccg gcctcaagtg
     8041 cctcaaggcc ggcggcgcat tctacgcctt tcccgacgca agggtagcca tcgccaggct
     8101 gcacgccgcc ggcaagatca aggaagccac cgatctggct ctatccgact acctgctgga
     8161 atcggtcggc gtggcggtgg tgccgggttc tgctttcggc gccgatggct atttccgcgt
     8221 ctctttcgcc acctcgatgg acaacctcag gaacgcgctg gaccggattg ccgaagcact
     8281 ggcttgattg catccggaaa ataacaaggc cctgcaatgc agggccttgt tattttgacc
     8341 ttcactcatc gtatttcagg tacttgaagc gcacatcgtc gttgggcgta ccttccagcg
     8401 caccacctac cttgcccggc tgccagcaga tcacttcctc gatctttttc gccagcgcaa
     8461 aatccttgtc ggtgacgcct ttggcggtat gcgtgaccag cttgacaatg acaaaggcgt
     8521 aggaaacggt gagatctggg tggtgccagg cggcttccgc cagatggccg acggtgttga
     8581 ctaccatcag cgtggccttc cagccggcgg tcttgtattt gcggcgaatc caaccatcct
     8641 cgtagtacca gtgcggcaac tcggcggtca gccggctgcg aatttcctct tcggtatagg
     8701 catgttcagt catggcgtgc tccttgcagg gcttcagaat gccagcgtag gaatcggcgc
     8761 acccgccagt gccaatgcaa aatcggcata gccggacttg tcgacaacgt gtattggaca
     8821 ggccaaacct gtggcaaatt ccgtgaaagc ggcatccaga acaccctcct ccaccaattg
     8881 ccgcaacggc ggctgcaacc gggtaaactc gacgtgcttg accagcacca ggcggttggc
     8941 gccaatcttg cgcgccagcc aagcggccag gctgtccgag gtcacttccc agttcatagg
     9001 gatgctgtca tccgccagca ccatctggct gggtagccag acgatggcgc gatgctgcca
     9061 ggagcgttcg gcaatttcca gctcggagga tgccgtcacc aattgcggct gcaaggattg
     9121 caatgccaga ccgtactgct ccatgcccag tagcgccata tggtgtgccg ccacatcatc
     9181 gtagccaccc acggtttggg cgctgcgcac agtgtcggca aagatgccgc cgccggggac
     9241 aataacgacg cggccgtcgc tctgccttgc caacagatcc agccattgcc tcagctccgg
     9301 cgtgcctagc aggctgccac cgagcttaac cacccacatg aaattgttcc atcagttgca
     9361 gcatgctgct cgccttatcc caggtctcgc ggtattcctt atccatctcc gaatcggcga
     9421 cgatgccacc gccggcccag aagcggatgc tgccgccgga atagaccgct gtgcgaatgg
     9481 caatgctggt gtccatgttg ccatcgaaac cgatgtagcc gatggcgccg caatacacgc
     9541 cacgccggtg cggttccagt tcctcgataa tctgcattgc ccgcagcttg ggcgcaccgg
     9601 taatggatcc gcctggaaag cagccacgca gcaggtcgat ggcgtcacgc ccctccgcca
     9661 aggtgccagt gacagtgctc accagatgat gcacattggc gaagctctcc agttcgaaca
     9721 ggcgttccgc cttgaccgaa ccgactgcgc aactcttgcc gatgtcattg cgcagcaggt
     9781 cgacgatcat caggttttct gccctgtcct taaggctggc gcgcagttcg tcagcgttct
     9841 ccaggtcctg tgccagatca agggaacgcg gccgcgtgcc cttgatcggc ttggtttcga
     9901 cccggccgtc acgcactttg aggaagcatt ccggtgaagc tgacagtatc tgcgccgccg
     9961 gcgtattgag gtaggcggag aaaggcgccg ggctgatgcg gcgcagctgc aggtaggccg
    10021 tccacgcgtc gccttctgcc ttcgtactga aacgctgcgc aaggttgacc tggtagcagt
    10081 caccggcctg gatgtaatcc agcacgcgcc ggaatgctgc ggcataggtg tccatatcca
    10141 tattggaaga tatcagaccg gtggttttga aggtttctgc ctgagtcaca gcagacgcac
    10201 tgaacaatgc acgcaactca tcccagcgct gctgcgtgcg agcatcgaag ccctgcccta
    10261 ccagccaggc gcgacgctgt tcatgatcca gcactaccgc ccagtcgtag acccccaccg
    10321 ccatttccgg catatgctcg gcatccacgg cggtatccgg caaatgttcc agacgccgcg
    10381 ccaggtcgta ggaaaaatag ccgatggcgc ctccggcaaa tggcagctcg ccggcactgg
    10441 caggaaactg ccgcaaggtt cgctgcaaca gcatgaacgg gtcttccgag gaaatacgga
    10501 catcctcgcc ctggcggatt tcggttttcc cgcatcgagt caccagggtg atgaaagggt
    10561 cggcggcaag gatgtcgtag cgaccgtaac tgctgccagg acggccgctg tcaaggtaga
    10621 ccgcccacgg ccgctgggca atgtgcccga acagttcggc accgttggca cgataaggca
    10681 gctcggcaat catgcttcgt ttcttgccag ccaggccacg gcataggctg gcaagcatat
    10741 gccggcccac tcggcggttt ccgggacagc cgtcacccac tcagaggcat cgcggtattt
    10801 gcgaccgaga cggctcgcca gctcacgcac caggaagcgg ccggccccag ccccgatcag
    10861 gggagcctca tcgtccagca gagcatgtga caaagttcgt tcgacagcga attgcagcag
    10921 gtgcaactgc tgcgagcgca cggcaaatgc caattgccgc cattgcggca tgggcgcatc
    10981 ttgcaggtcg cgcccgacca tgcgcgccag acggcgggca ctggcttgcg gcgtcttctc
    11041 gccgccgtca gcggtgtccg ccatgtcgta gctttccggc aattccccag tcagccggta
    11101 gacgtccgcc atggtggcaa aatgctcggc agcaagacgt tgccattcac cggaaaatgg
    11161 tagttgttgt gctaccgcca tcactggcgt gcgtattgct ccggtataaa ccagttcgtc
    11221 gtaacgcatc cgttcggcat cagaatagcc acgggtagcc agctggccat gatgaagcac
    11281 caccagatcc gcggtggtac taccgacatc gatgaatagc ccctgcgcgc ccctggctgc
    11341 caggaaagcc gcgctggcat gccagttggc ggaggcgata tcggccaggt gctccagcac
    11401 ggcatgcgtc gccaccaaac cgctacggcc ggcatagata agcaaatccg catcgcccag
    11461 acgatccgcc atcagttgtg tcagatgcgc cacgccttcg ccgcggctgg gaaagatgtc
    11521 cgcgagttcg ccggtcatgg tcaccgtatg ccgtaacggc gaatctccca acccggccat
    11581 caccgccgcg atggcctgct ccagatgcgc cgatccgcgc cacaatgggc agggctgctg
    11641 gaacacacgc agcgcacggc catcggcatc aaccagcgcc gccttcaaat gcgcaccccc
    11701 gagatcccag ccgagtgtta agggtagctt gttattcatt gagggatatt gtcacaacat
    11761 tgcgctcgac gcgcggaagc gttt
//
LOCUS       DAOMFA010000038        11569 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_553_length_11569_cov_5.614730, whole genome shotgun sequence.
ACCESSION   DAOMFA010000038 DAOMFA010000000
VERSION     DAOMFA010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 11569)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11569)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11569
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_553_length_11569_cov_5.614730"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..705)
                     /locus_tag="PKW44_03260"
     CDS             complement(<1..705)
                     /locus_tag="PKW44_03260"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HQR50638.1"
                     /translation="MFKLGRNIHRNVSAGDSVDVSEADGVRSLHLGSPTVQSSMRLSA
                     PHDLELAYTRGIMTFLLFAPEVRKVLAIGLGGGSIPRFIHYYLPRLHTTVVDINPQVI
                     AVARSHFLLPPDDERLHVIEGDGAQYLKEHPGRAELLIIDAFDSRGVPADLCSQDFFD
                     SCAEAVTADGLMAINLWGSDRNYDVYLQRIERSFSHRVLVLPTGRPGNVVVFGFRRIP
                     ADLRWANLRERARLLQE"
     gene            complement(751..1323)
                     /locus_tag="PKW44_03265"
     CDS             complement(751..1323)
                     /locus_tag="PKW44_03265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR50639.1"
                     /translation="MITTNKPTPDRVELPPAKPVEPPPLPPTPSPRPDAPTDMMSYVN
                     AARERRRQSEDYAARINAEAVARERKPSEDEVRNEIIKSNLKAGTNGIFRIISVGPYN
                     AQFSFMGWTTDYSNARRELVLVEAGPGEDIRRNIARKMIELIRRHYSGDFNWESLRLG
                     RVVVLSARQEDGPGLEDFLILEFFQGDALR"
     gene            complement(1658..3502)
                     /locus_tag="PKW44_03270"
     CDS             complement(1658..3502)
                     /locus_tag="PKW44_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HQR50640.1"
                     /translation="MNVFYEEESSFKAASVMAENPGSLQVEAISGKRSKIKAANVLLR
                     FEGTLAGFMEQAQTEAETLEADFLWECSGEEEFGFEELAREYYGHAPSAIEAAAVAIS
                     LHAAPIYFYRKGKGRYKAAPEDNLKAALAAQERKRLQAEKVAGYVEQLKAQQLPAEFG
                     GKLDMLLYEPDKNALEYKALEEASAGTGLSHLKLLEACGAIASPHDYHFGAFLREHFP
                     AGVHFEANEAIRLPDNLDQAEVEAFSIDDAATTEIDDAFSVQLLPNGNSRVGIRIAAP
                     ALGIPVNSALDAVAMKRLSTVYMPGNKITMLPEAAITPFSLHAGEWRPALSLYLDIAH
                     DLTIINCASRLEAVKIADNLRHDELEPHFNEGNWATPNDHPYWGKLELLHRLAESLEK
                     TRGKHDPDRTPHVDYNFYVENGLVRITERKRGAPMDKLVAELMIYANAYWGGMLAEHG
                     IPGIYRAQQGGKVYMTVSAEPHQGLGVAQYAWSTSPLRRAVDLINQRQMLALLQGTEP
                     AYGKNSEALTTAMRDFDLTYNAYNEFQTRMERYWCLQYLVQQNITEPAATVWRENLVR
                     LDGMPTITKVHNLPDLAPGSRVQLEIKRIDSLLMELDCRFKTVLSVEA"
     gene            complement(3666..8639)
                     /locus_tag="PKW44_03275"
     CDS             complement(3666..8639)
                     /locus_tag="PKW44_03275"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HQR50641.1"
                     /translation="MTIEFDIGPLTWVKGEIDQALMQALENVKEFAGSPADLTPLRFC
                     QTHVHQVAGALAMVGLDGAQRFSTEIEQYVTALEKQQLDPSSQNLDALADALNALVRY
                     VNALVEGAPDIPLKLFPEFKALLNARGVMQVMESELFFPDLNHIPPTGPASDVPQNLP
                     AYLDEQCQQYRQGLVQWLRDAGDQAALQNMLEALLAVERVQILAAQKALWWASAGLLE
                     AVAAGDVAVTQNLRHLCTKLERQLHYLSRGDVRPGSRLLRDVLYFVAVSQPVSARARE
                     IKRVFDLDDYHAALAITGGGASGQAVSAGDADLFATLRSSLDELKASWNKVSAGDTTQ
                     LEDFRQKSANLSTLAADIHWPELQQSLTDIQDCAAELTSRGKIPEPLFSIEVATSLLM
                     LESVLKLTGKFREDELQALLSQTAAMRGVLLGETREVAVKESGHAETLAVLSQVADEI
                     RANLQHIEQLLDVFFRNAETRGELQGASSLFKQVAGAFEMLDQHTAATVVRDCWSLAE
                     RFSQPSYLAENSEYELVAESISALEFYLAQLLEGSGDAVRAIEALPARLQVCLKAAPA
                     YATEHVAIEAARDAAGVEPEKLVDSALDAELLDVYLTEAGEVLETIAENLQSLRSKRD
                     DHEALVTVRRAFHTLKGSGRTVGLVAMGDVAWAVEQLLNQMLEMRAAADDLVLDFVDE
                     VAREFHEWVQQLTTSGMAALSPSPWQARAEALRHHLTTIEAVSEAIVEAAAKPEIQAP
                     DGEDIVIGDTFRVGRKLFALFLEEAYANLGVLFTAYAAARKHSEPLAAETTRAAHTLA
                     GVSRTIGITALADASAALESWLEKIAPRLQQLADEELRLFDQSLAALQEILLQTANRT
                     LPKPATRVVSELKSATGEPLAAEQLESQLSGEPSPVMEADIEVAEAGIITAATQNAQE
                     NTGVDRQLLDVFIEESNELMPMIGKQLREWQESPEAGASQQALQRALHTLKGSARMAG
                     ATELGDALHDLETQVVKAAEKGVPTARNFTDFFRRYDAANDHLSALHQQVETPVAGGR
                     TEGLPQQDEQIEPIAVTPMMRVRSEVIDQLVNEAGEVSIARSRIERQMETFKQALLEL
                     TDSVIRVRGQLREVEIEAETQMQSRLLQIEETHETFDPLEFDRYTRFQELTRMMAESV
                     HDVSTIQQGLLYNLSETEAALIEQSRMTKELQQGLMRTRMVRFDNVTERLHRIVRQTG
                     GELGKPATLHLRGGEVEIDRSVLEKMVGPLEHLLRNAVAHGLEDAKQRASLRKPKVGK
                     IELHVRQEGNEIVLSLSDDGRGLDFDKIKAKAARLGMFDAAMESDPSSLLTLIFESGF
                     STSEEVTQVSGRGVGLDVVRNDITSLGGRIEVVSESGKGCTFTVYLPLTLAVNQTVLV
                     RTGEDEYLLPATVVEQVQKLKPDALAAAEQRGAVVWAGNDYPLHHLSRLLGRPVQPEP
                     QAYMPVMLLRSGARRIAMVVDEVLGNREIVVKNVGPQLAGMSSIAGATVMGDGKVMLI
                     LNPIQMAFRESLVSETVYRAQAEDQAEHKHVPTVMVVDDSLTMRKVITRLLTREGYHA
                     LTAKDGVDALQQLQSVLPDVVLLDIEMPRMNGFELARAIRGDARTAHLPLIVISSRTA
                     DKHRNYAEELGVNVYLGKPYQEEELLSHIAEFMQASAVQAKAESTAD"
     gene            complement(8662..10332)
                     /locus_tag="PKW44_03280"
     CDS             complement(8662..10332)
                     /locus_tag="PKW44_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HQR50642.1"
                     /translation="MALQDLLQELKSEGAGALLKRLFSRASGAPHAAKSPVNIRMAAL
                     LVLFVLLAASFLGLDLAQRQHDAAYLARVSQAQLGAQGVAKDAQLVASGAQNFSGELQ
                     RNLGTVSSAVAALRDEGGIFGYGDSLSVPLQELGTQWQNIERQARATADAGGAADAAK
                     GLASSADQFLAAGDTLASAVRDNIKSKLWLILAVVFAVLAATLMITFGQQQVKSAQSH
                     FESVEQQNLKNQEAVLRLLDEMGDLAEGDLTVTAEVSESITGAIADSINYTIDSLRTL
                     VEEINHATEQVTAASGEAQAITTRLQAAALKQSQQIEDAGTAVQQMARSIHEVSANAN
                     QSAGVAQRSLQAASQGAVAVQNTISGMNDIRTQIQETSKRIKRLGESSQEIGEIVELI
                     SDITEQTNILALNAAIQAASAGEAGRGFTVVAEEVQRLAERSSEATKQIGAIVKTIQQ
                     DTNDAVVAMERSTEGVVEGAKLSDAAGQALNEIGKVTANLAQLIASISQATQAQTEIE
                     QKVTGLMQEIQAITSQTTEGTKQTAGSIGQLADLAEELKASVSGFKLA"
     gene            complement(10426..10950)
                     /locus_tag="PKW44_03285"
     CDS             complement(10426..10950)
                     /locus_tag="PKW44_03285"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HQR50643.1"
                     /translation="MARTLDLRKYQEDILGRLKERAQPGEVASSSRLGVKVGRSLLLV
                     SLEDISEVAPVPEIHPVPLAKPWFLGMSNVRGNLYGVSDMAQLAGDTPTTLTASSRVL
                     LSHQKYKANVALLVNALLGLRNLDQMQEQEIADEGPYGFGGRQFKDSAGDTWRELNMR
                     DLLVNPDFLKVGIG"
     gene            complement(10951..11316)
                     /locus_tag="PKW44_03290"
     CDS             complement(10951..11316)
                     /locus_tag="PKW44_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018078431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQR50644.1"
                     /translation="MPIKKIMIVDDSATDRFYLNEMLTRNGFEVSQAESGDVALERVK
                     VELPDLVIMDVLMPGLNGFQTTRALTKAPETAHIPVLMCTGKEQLTDRVWALRQGARD
                     CVIKPVEEADLLGKIAALG"
     gene            complement(11338..11565)
                     /locus_tag="PKW44_03295"
                     /pseudo
     CDS             complement(11338..11565)
                     /locus_tag="PKW44_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634205.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
BASE COUNT         2236 a         3585 c         3233 g         2515 t
ORIGIN      
        1 ttcctgcagg agtctggcgc gctcccgcaa gttggcccag cgcaagtctg caggaatgcg
       61 ccggaagccg aacaccacga cgttccccgg tcggccggtc ggcaagacca gcacgcggtg
      121 ggaaaaactt cgttcgatgc gttgcaggta gacatcgtaa ttcctatcgc tgccccacag
      181 attgatcgcc atcagtccat cagccgtcac cgcctcggcg caactatcga aaaaatcctg
      241 cgagcacaga tccgccggca cgccgcggct atcgaaagcg tcaatgatca acaattctgc
      301 ccggcccggg tgctccttca aatactgcgc gccgtcacct tcgatgacat gcaatcgttc
      361 atcatccggc ggcagcagaa aatggctgcg cgctacggca atcacttgcg gattgatgtc
      421 gaccacggtg gtgtgcagcc gcggcaggta atagtggatg aacctgggga tggaaccgcc
      481 gccaaggcca atagcaagaa ccttgcggac ttcaggcgcg aacaggagga aggtcatgat
      541 gccgcgggta taggccaact ccaggtcgtg tggcgccgaa agccgcatcg agctttgcac
      601 cgtgggcgag ccgagatgca gcgaacgcac gccgtcagcc tcgctgacat ctacgctgtc
      661 gccggcggaa acgttgcggt ggatgttgcg gcctagcttg aacataagct ccccatcaat
      721 gacagaccca aattctagcc caggcacgcc tcatcgcaga gcatctccct gaaagaattc
      781 caggatcaga aaatcctcca gtccggggcc gtcttcctgg cgggccgaca acaccactac
      841 tcgccccaat cgcaaagatt cccagttgaa atcgccgctg tagtggcggc gaatgagttc
      901 gatcatcttg cgtgcgatgt tgcgccggat atcctcgccc ggcccggctt cgaccagaac
      961 caattcgcgg cgtgcgttgg agtagtcggt ggtccagccc atgaagctga attgcgcgtt
     1021 gtacgggccc acgctgatga tccggaaaat gccgttggtg ccggccttga gattactctt
     1081 gataatttcg ttcctgactt catcttcgct gggcttgcgc tcccgtgcga ccgcctcggc
     1141 gttgatgcga gctgcataat cttcggactg gcggcggcgc tcccgtgccg cattgacgta
     1201 actcatcatg tcggtcggcg catccggcct gggcgaaggc gttggcggca aagggggcgg
     1261 ctcgaccggc ttggcgggag gcaattccac ccgatcgggt gtcggcttgt tggtggtgat
     1321 caccgtcggc ggctttctga cggcttttgg ttgaggtttt ggcagcggtt ttgcctccgc
     1381 tggcggctcg ggctgcggca agggcggtgg gagtggccgg ggcttgtccg gcaggttcag
     1441 actcaccgtg aaaggcgtcg cctgcgaggg ggggttatcc agttccagaa gtcttgattt
     1501 tagaaaaaat aacgcgatca agtgaacaag cagggaaaat accactgcaa acagcatact
     1561 gcgacggatg cggaactcta tgaaactttc cctgcggaga gggactttcc agctaacggt
     1621 cgatggtaac aatttcagct tcttgcatta aaccggttca ggcctcgacc gacagcaccg
     1681 tcttgaagcg gcagtccagc tccatcagca gcgaatcgat gcgcttgatt tccagttgca
     1741 cgcggctgcc cggtgccaga tccggcaggt tatgcacttt ggtaatggtg ggcatgccat
     1801 ccagtcgcac cagattctcg cgccagacgg tagcggcagg ttcagtgata ttctgctgaa
     1861 ccaggtactg caagcaccag tagcgctcca tgcgggtctg gaactcgttg taggcgttgt
     1921 aggtcagatc aaagtcgcgc atagccgtgg tcagggcttc gctgttcttg ccgtaggccg
     1981 gttccgtgcc ttgcagcagc gccagcatct ggcgctggtt gatgaggtcg accgcccttc
     2041 tcaatggcga ggtactccat gcatactgcg ccacgcccag cccctgatgc ggttcggcac
     2101 tgaccgtcat atagaccttg ccgccctgct gcgcacggta gatgccgggg atgccatgtt
     2161 ccgccagcat gccgccccag tacgcgttgg cgtaaatcat cagctctgcc accagtttgt
     2221 ccatgggcgc gccgcgcttg cgctcggtga tgcgcaccag gccgttttcc acgtagaagt
     2281 tgtagtccac gtgcggtgtc cggtcgggat cgtgcttgcc tcgcgtcttt tccagagatt
     2341 ccgccaggcg atgcaacagt tccagctttc cccagtaggg atgatcgttg ggtgtggccc
     2401 agttgccctc gttgaaatgc ggttccagct cgtcgtggcg caggttgtcg gcgattttca
     2461 ccgcttccag ccggctcgca cagttgataa tggtcaggtc gtgagcaatg tccaggtaga
     2521 gcgacagtgc cggccgccat tcgccggcgt gcaggctgaa cggggtaatg gccgcttccg
     2581 gcagcatggt gatcttgttg cccggcatgt agacggtaga caaccgcttc atggccaccg
     2641 catccagggc cgaattcaca ggaatgccca gcgccggcgc ggcgatgcgg atgccaaccc
     2701 ggctgttgcc attggggagc aactgcaccg agaacgcatc gtcgatctcg gtggtggcgg
     2761 catcgtcaat gctgaaagct tcgacctcgg cctgatccag gttgtccggc agcctgatcg
     2821 cttcattggc ttcgaaatgc acgccggccg ggaagtgctc gcgcagaaag gcgccgaaat
     2881 ggtaatcgtg tggtgatgcg atggcgccgc aggcctccag cagcttgagg tggctcaggc
     2941 cggttccggc ggatgcctct tccagcgcct tgtattccag tgcgttcttg tccggctcgt
     3001 acagcagcat gtccagtttg ccgccgaact ccgccggcaa ctgctgcgcc ttcaattgct
     3061 ccacgtaccc ggcgaccttt tccgcctgca ggcgcttgcg ctcctgggct gccagcgcag
     3121 ccttgaggtt gtcttccggc gccgccttgt agcgtccctt gcccttgcgg tagaaataga
     3181 taggtgcggc gtgcaaactg atcgcgaccg ctgccgcctc gatagcgctg ggcgcatgcc
     3241 cgtaatattc ccgcgccagt tcctcgaaac caaattcttc ctcgccgctg cattcccaca
     3301 ggaaatctgc ctccagcgtc tcggcctcgg tctgcgcctg ttccatgaag ccagccagcg
     3361 tgccttcgaa cctcagcaat acgttggctg ccttgatttt ggagcgcttg ccggaaattg
     3421 cctcgacctg cagcgagccg ggattctcgg ccatgaccga agccgctttg aaactgctct
     3481 cttcttcgta aaaaacgttc aaaagcttcc ttttgccaac cgggagatta aaagtgagac
     3541 ctatcggata tacgatgtgc cccggcaaga acaaagcttg ccaggcaacg gtgaattata
     3601 acaactgagg agggaacgag cctgatgaaa ccgattgcgc cccggttggc acgaattctt
     3661 tcgggttaat cggccgtgct ctcggctttg gcctgtacgg cgcttgcctg catgaattct
     3721 gcaatgtgcg aaagcagctc ttcttcctgg taaggctttc caaggtagac gttgacacca
     3781 agctcctcag catagttgcg gtgcttgtcc gcggtacgcg agctgatcac gatcagcggc
     3841 agatgggcag ttctggcgtc gccgcggatt gcacgcgcca gttcgaaacc gttcatgcgc
     3901 ggcatttcga tgtccagcaa caccacgtcc ggcaacacgg attgcagttg ctgcagggca
     3961 tcgacaccat ccttggcggt gagtgcgtga tagccttcgc gcgtcagcag gcgcgtgatg
     4021 actttgcgca tggtgagcga atcgtcgacc accatcaccg taggcacatg cttgtgctct
     4081 gcctggtctt ccgcctgggc gcggtaaaca gtctctgata ccaatgactc gcggaatgcc
     4141 atctggatcg ggttcaggat cagcattacc ttgccatcac ccattaccgt ggcaccggca
     4201 atgctgctca tccctgccaa ctgcgggccg acgttcttga ccacgatttc gcggttgccg
     4261 agcacttcgt ctaccaccat ggcaatgcgg cgggcaccgc tgcgcaacag catgaccggc
     4321 atataggcct gcggctccgg ttgtaccggt ctgcccagca agcgcgacag atgatgcaac
     4381 ggataatcat tgcctgccca caccaccgcg ccgcgctgct ccgcggccgc cagcgcgtcc
     4441 ggcttcagtt tttgcacctg ctcgaccacc gtcgcaggca gcaggtactc gtcctcgccg
     4501 gtgcgcacca gcacggtctg gttgaccgcc agggtcagcg gcaggtagac ggtaaaggtg
     4561 caccccttgc cgctctccga aaccacttca atgcggccgc ccagactggt gatgtcgtta
     4621 cgcactacat ccagaccgac gccgcggccg gacacctggg tcacttcctc tgacgtggaa
     4681 aaaccggact cgaaaatcag tgtcagcaat gacgagggat cgctttccat ggccgcatcg
     4741 aacatgccaa gcctggctgc tttggccttg atcttgtcga agtcaaggcc gcgcccgtca
     4801 tcgctgagcg agaggacgat ttcattgcct tcctggcgca cgtgcagttc aattttccca
     4861 accttgggtt tgcgcagcga agcgcgctgc ttggcgtctt ccaggccatg cgccacggcg
     4921 ttgcgcagca aatgttccag cggcccgacc atcttctcca acacactgcg gtcgatttca
     4981 acctcgccac cgcgcaggtg cagggtggcg ggcttgccca gttctccgcc agtctggcgc
     5041 acgatacggt gcaggcgctc ggtgacgttg tcgaaacgca ccatgcgcgt ccgcatcaaa
     5101 ccctgctgca gttccttggt catacgcgac tgctcgatca gcgctgcctc ggtctcgctc
     5161 aggttgtaca gcaagccctg ctggatggtg gatacgtcgt gcacgctttc cgccatcatg
     5221 cgggtcagtt cctggaagcg cgtatagcgg tcgaactcca gcgggtcgaa tgtttcgtga
     5281 gtttcctcga tctgcaacag gcgcgactgc atctgcgttt cggcctcgat ttcgacctcg
     5341 cgcaattggc cgcgcacgcg gatcacgctg tcggtcagtt ccagcagcgc ctgcttgaag
     5401 gtttccatct gccgttcgat gcgcgagcgg gcaatactga cttcacctgc ctcattgacc
     5461 agttggtcga tcacttcaga gcgcacccgc atcatcgggg tcacggcgat aggttctatc
     5521 tgctcgtcct gttgcggcag gccttctgtc cggccgcccg caacaggggt ttcgacctgc
     5581 tggtgcaatg ccgacaaatg atcgttggcg gcatcatagc gccggaaaaa atcggtgaag
     5641 ttgcgcgcag tgggaacgcc tttttccgct gccttgacca cctgagtttc cagatcgtgc
     5701 agcgcatcgc ccaactctgt tgcgccggcc atgcgcgcac tgcctttaag cgtatgcagg
     5761 gcgcgttgca gtgcctgttg ggaggctccg gcttcaggcg attcctgcca ttcacgcagt
     5821 tgcttgccga tcattggcat caactcgttt gactcttcaa tgaaaacatc cagcaactgc
     5881 cggtcgaccc cggtattctc ctgagcattt tgcgtggctg ctgtgatgat cccagcctcc
     5941 gcaacctcga tatccgcctc catcaccggc gatggctcgc cggacaattg ggattcgagt
     6001 tgttccgccg caagcggttc gccagtcgct gacttcaact cactaacaac cctggttgct
     6061 ggcttgggca gcgtacggtt ggccgtctgg agcagaattt cctgcaatgc ggccagcgat
     6121 tggtcaaaca ggcgcagttc ttcatccgcc agttgctgca gccgcggcgc tattttttcc
     6181 agccatgatt ccagggctgc actggcgtcg gccagtgcag ttatccctat cgtgcgcgat
     6241 accccggcca gtgtatgcgc agcgcgggtg gtctctgccg caaggggttc cgagtgcttg
     6301 cgggcggctg cgtaagctgt gaaaagcacc ccgaggttgg cgtatgcctc ctccaggaat
     6361 agcgcgaaca attttcggcc gacccggaat gtgtcaccaa tgacgatgtc ctcgccatcc
     6421 ggcgcttgta tctctggctt ggcagcggcc tcaacaattg cctccgaaac agcctcgatc
     6481 gttgtcagat gatggcgcag ggcctccgct cttgcctgcc atggcgatgg cgacaacgct
     6541 gccataccgc tggtggtaag ttgctggacc cattcatgga actcacgggc aacctcgtcg
     6601 acaaaatcga gaaccagatc atcggccgcc gcgcgcattt cgagcatctg gttcagcagt
     6661 tgttcgaccg cccacgccac gtcgcccatc gcgaccaggc cgacggtacg gccgctgccc
     6721 ttgagagtgt ggaatgcccg gcgcacggtt accagcgctt catggtcatc ccgcttgctg
     6781 cgcaacgact gcaggttctc cgcgatagtt tcgagaactt cgccggcttc cgtcaggtag
     6841 acatccagca gttcggcatc cagtgcgctg tctaccagtt tctctggttc aaccccggca
     6901 gcatcccggg cggcctcgat ggcgacatgc tccgtcgcat aagccggcgc agccttcagg
     6961 cacacctgca gacgggcagg taacgcctcg atagcgcgta cggcatcgcc gcttccctcc
     7021 agcagctggg caagatagaa ttccagggcg ctgatgctct cggcaaccag ctcgtattcg
     7081 ctattctccg caaggtagga aggttgcgaa aaacgctctg ccaaggacca gcaatccctg
     7141 acaaccgtgg ccgccgtatg ctgatcgagc atctcgaagg caccagccac ctgcttgaac
     7201 aggctggaag cgccctgcag ttccccgcgg gtctccgcat tgcggaaaaa cacgtccagc
     7261 aactgctcga tgtgctgtaa attggctcgg atttcgtcgg ctacctggga caataccgcc
     7321 agggtctcgg catggccgga ttccttgaca gcgacttccc tcgtctcgcc aagcagaacg
     7381 ccgcgcatgg ccgcggtttg cgatagcagc gcctgcaatt cgtcttcgcg aaatttcccg
     7441 gtaagcttga gcacgctttc cagcatcaac aggctggtgg ccacctcgat cgagaacaaa
     7501 ggttcgggaa tttttccgcg gctggtcagt tcggcagcgc aatcctgtat atcagtcagt
     7561 gattgctgta actccggcca atggatatcc gctgccagcg tggaaaggtt cgcgctcttc
     7621 tggcggaaat cctcgagttg agtggtgtcg ccagcggata ctttgttcca actggctttt
     7681 agttcatcca ggctgctacg caacgtcgca aaaagatctg cgtcgcctgc agaaacggct
     7741 tgcccggacg cgccgccgcc cgtgatcgcc aaagccgcat gatagtcgtc aaggtcgaat
     7801 acacgcttga tctcgcgcgc tcgcgcggat accggttggc tgaccgccac aaagtaaagc
     7861 acgtcccgta acaaacggct gcccggacgc acatcccccc gtgacagata atgcagttgc
     7921 cgctccagtt tggtgcagag gtggcgcagg ttttgagtaa cggcaacatc acctgcggca
     7981 accgcctcca gcaggccggc ggacgcccac cacaaggcct tctgtgccgc cagtatctgt
     8041 acacgttcta cagcgagcaa tgcctcgagc atgttctgca atgcagcctg atccccggca
     8101 tcgcgcaacc attgcaccag gccttggcga tactgctggc actgctcgtc caggtaggct
     8161 ggcagattct gcggtacatc gcttgcaggg ccagttggcg gaatgtggtt gaggtcagga
     8221 aagaacagtt cactttccat gacctgcatc acgccgcgcg cgttgagcaa tgccttgaat
     8281 tcagggaaaa gttttaacgg tatatccggt gcgccctcga ccagcgcatt gacgtacctt
     8341 accagcgcgt tcaaggcatc agccagggca tcaagattct gcgaggaagg gtcaagctgt
     8401 tgcttctcga gcgcagtgac atactgttcg atctctgtcg agaaacgctg cgccccatcc
     8461 agcccgacca tggctagggc gccagccacc tggtgaacat gcgtctggca aaaacgcagc
     8521 ggcgtcaggt cggccggaga accggcgaat tccttgacgt tttccaacgc ctgcatgagc
     8581 gcctggtcaa tctccccctt cacccaggtc agcggcccga tatcgaactc gatggtcacg
     8641 atagtctgct agttgatctg gttacgcgag cttgaaaccg gacacggagg ctttcagttc
     8701 ctcggccagg tcggccaact gtccgataga gccggcggtc tgcttggtac cttcggttgt
     8761 ttgcgaagta atcgcctgaa tttcctgcat caggccggtt accttttgct cgatttcggt
     8821 ctgcgcttgc gtcgcctgcg aaatggaagc aatcagttgc gcaagattcg ccgtcacctt
     8881 gccaatttca ttcagcgcct gaccggcggc atcggacagc ttggctccct cgaccacgcc
     8941 ctcggtgctg cgctccatgg ccaccaccgc gtcattggta tcttgctgaa tggtcttcac
     9001 aattgcacca atctgcttgg tcgcttccga agagcgttcc gcaaggcgtt gcacttcttc
     9061 cgccaccacc gtgaagccgc ggccggcttc acccgccgac gccgcctgaa ttgcggcatt
     9121 caaagccaaa atattggtct gctcggtaat gtcggagatc agctccacga tttcaccaat
     9181 ttcctgagaa ctttcaccca accgcttgat acgttttgat gtttcctgga tctgagtgcg
     9241 gatatcgttc atgccggaga tcgtgttctg caccgctacc gcaccttgcg atgctgcctg
     9301 cagggagcgt tgcgccacac cggcagactg atttgcattg gcagacactt cgtgaatgga
     9361 tcgcgccatc tgctgcacag ctgtaccagc atcctcgatc tgctgtgact gtttcaatgc
     9421 agccgcctgc aaccgggtag taatggcctg cgcctcgccg gaagcggctg tcacctgctc
     9481 ggtggcatga ttgatttcct caaccaaggt gcgcaagctg tcaatcgtat aattgatgga
     9541 gtcggcaatt gcgccggtga tactctcgct cacctccgca gtcacggtca aatcaccttc
     9601 ggccaggtcg cccatttcgt cgagcagtcg cagcacggct tcctggttct tgaggttttg
     9661 ttgctccaca ctctcgaaat gcgattgggc cgatttcacc tgctgctgcc cgaaagtaat
     9721 catcagcgtg gctgccagca cagcgaatac tacggccagg atcagccaca gcttgctctt
     9781 gatattgtcg cgcactgcgc tggccaacgt atctcctgca gccagaaact gatcggctga
     9841 cgacgccagc ccctttgcag catcggctgc gccacctgca tcggccgtgg ccctggcctg
     9901 tctttctatg ttttgccact gcgtgccaag ttcctgcaga ggaacgctca agctgtcgcc
     9961 atacccgaaa attccgccct catcgcgcaa tgcggcaaca gcactggaga ccgttcccaa
    10021 gttccgctgc aactcgccgg aaaaattctg ggctccgctc gccacaagct gcgcatcctt
    10081 ggcgacgcct tgcgcaccga gctgtgcctg cgatacccgg gcaagataag cagcgtcatg
    10141 ctgcctttgt gccagatcca gccccaaaaa acttgccgcc agcaacacaa acaacaccag
    10201 caatgcggcc atcctgatat tgaccgggga cttcgccgca tgcggcgcac ctgaggcccg
    10261 cgaaaacagt cgcttgagca gggcccccgc cccttcgctc tttaactcct gcaacaagtc
    10321 ttgtaacgcc atgacactcc cccgaaagtt ctttaaatct gtctaatcta caaacccgct
    10381 cgcccgtgta tcccactgct ccggccgaac ccctggacca ccgctctagc caataccgac
    10441 ctttaaaaaa tcaggattaa ccagcaaatc gcgcatattc agttcgcgcc atgtatcacc
    10501 agccgaatcc ttgaactggc gaccgccgaa cccgtacggc ccctcatctg caatttcctg
    10561 ctcctgcatt tgatccagat tgcgcaggcc aagcaaagcg tttaccagca aggcgacatt
    10621 ggccttgtat ttctggtgac tcaacaacac tcggctggac gccgtcagcg tcgtgggcgt
    10681 atcgccggcg agctgagcca tgtcgctcac accgtataga tttccacgta cgttagacat
    10741 cccgagaaac caaggcttgg ccaggggcac cggatgaatt tccggtaccg gggctacttc
    10801 gctgatatcc tcaagactga caagcagcag gctccgcccg actttgacgc ccaaccggct
    10861 gctggacgcc acctctccgg gttgcgctcg ctctttcagc cgaccaagaa tatcttcctg
    10921 atacttgcgc agatctagtg ttcttgccat ttatcccaac gccgctatct tgccgagcaa
    10981 atctgcctct tccaccggct tgatgacgca atccctggcg ccctgccgca atgcccagac
    11041 acggtcggtc aactgctcct tgccagtgca catcagaacg ggaatatgcg cggtctcggg
    11101 cgccttggtt aaggcgcgtg tggtctgaaa accattgaga ccaggcatca acacgtccat
    11161 gatgaccaag tccggcaact ccaccttgac tcgctcaagc gccacgtcgc cggattccgc
    11221 ctggctgacc tcgaagccgt tacgggtcag catttcgtta agatagaaac ggtccgtggc
    11281 agagtcgtcc acgatcatga ttttcttgat gggcataacc tgaatccgat cttgaagtta
    11341 attcttgcgg gtatgacgca ccacagcatc cagcaggctg tcagcagtaa agggcttggt
    11401 gaggtattcg tcggagccaa ccatgcggcc acgggcacgg tcaaacaggc catccttact
    11461 ggacagcatg atgaccggcg tgtttttgaa ccgctcgttc tttttgatgc gggtgccggt
    11521 tttatctacg tcgagtcgcg gaatcatgat gtcgtcaacg atgatttac
//
LOCUS       DAOMFA010000039        11487 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_563_length_11487_cov_4.827852, whole genome shotgun sequence.
ACCESSION   DAOMFA010000039 DAOMFA010000000
VERSION     DAOMFA010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 11487)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11487)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11487
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_563_length_11487_cov_4.827852"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            85..1101
                     /locus_tag="PKW44_03300"
     CDS             85..1101
                     /locus_tag="PKW44_03300"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HQR50645.1"
                     /translation="MIYEALRPLLFRIDAERAHHLTLQALQASYDLGLSRWLFPAPPP
                     CRPRQVMGLTFPNPVGLAAGMDKNGAHVDALAAFGFGFIEVGTVTPRPQPGNPKPRLF
                     RIPQARGIINRFGFNNDGVDALVENVRKARYRGILGINIGKNFDTPIEGATEDYLICL
                     RKVYAHASYVAVNISSPNTRNLRQLQDKEALQGLLAALKQEQAKLADVHGKYVPIALK
                     IAPDLETAQIVEIADLLLAHKMDAVIATNTTLSREGVESLPNAQEAGGLSGVPVRLKS
                     TAVIKQLSLALGKDIPIIGAGGILEGLDAEEKIKAGASLVQVYSGLVYRGQALVPEIC
                     RALG"
     gene            complement(1122..2786)
                     /locus_tag="PKW44_03305"
     CDS             complement(1122..2786)
                     /locus_tag="PKW44_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HQR50646.1"
                     /translation="MIRETISVIRDFGRMREIAGIMIHYGLGDFVKQLGLGNITERAG
                     NMLNRHQSEEIMHLEPAVRVRLAIQELGPTFVKLGQVLATRPDIFPPDWIAEFSKLQD
                     KVPPEPFEALLPELEKALGRSPFEVFQELQTEAIAGASIAQVHLAKLQDGTKVVLKVR
                     RPNVQGRIDADLRLLSHLARLLESEMPESRRFQPGKIVGQFAKSLRRELDLAMEARNT
                     DRFAHNFADDPDVVFAKVHWEHTSEHLLVMEYIDGIPGNDLDSALLSGLNLKILGARG
                     ADAVLKMVLVDGFFHADPHPGNVFYLPGNRLAIIDCGMVGRISTERRDQIADLLAALV
                     AKDIHALRDILIAWAGDAPIDDDKLSADVDELIFNYESAPLKHVRFSVLLNDLTTVMR
                     ENHLVVPPDLTMLFKALITLEGLGRQLDPDFQIVHHLTPFVKKIIVERYMPKAIAKRG
                     WRNVSGLLSLATDLPRDLSRLLREARRGRVKIDLDLKRLDHFGHQLDQSTNRLTLGII
                     TSALIIGSSIVMTVRGGTVLFGFLGFFLASLFGIWLIISIWRSGRD"
     gene            complement(2811..3473)
                     /locus_tag="PKW44_03310"
     CDS             complement(2811..3473)
                     /locus_tag="PKW44_03310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR50647.1"
                     /translation="MSDKKEFSLEQIKDSARNLLRSGENVREKLHELTVKALTQRQLA
                     EQEIREVIGAITEGVSLGAAERAEDVRVALGDALNGMDDALGHAAEAMQLAWGEVSSH
                     AQEFAEQDLKQGISELKNLEQVFLETVSRVGQGASGLVKQEMAALAEHARHAGTGTGE
                     RVKAVAEDLGNRLRATAQQAGDAGKNAAREVGARVAALASRKLSEIATRIADKAERLK
                     QK"
     gene            complement(3543..4442)
                     /gene="hemF"
                     /locus_tag="PKW44_03315"
     CDS             complement(3543..4442)
                     /gene="hemF"
                     /locus_tag="PKW44_03315"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HQR50648.1"
                     /translation="MDFDAVKDYFTDLQNRIVEALQVAEGKQFLHDSWQRPEGGGGVS
                     CVLEEGNVLERGGVNFSHVMGDKLPPSASAGRPELAGRGFEAAGVSLVLHPRNPYAPT
                     VHLNVRCFMAKKAGEAPVWWFGGGMDLTPYYGFEEDAVHFHRTCKLALDPHGAELYPR
                     FKQWCDDYFTLKHRKEPRGIGGVFFDDFNELGFERSFAMMKSVGDAFLHAYLPILQRR
                     KDLEYGERERDFQAYRRGRYVEFNLVYDRGTLFGLQSGGRTESILMSLPPIVKWRYDW
                     RPEAGSAEAKLYTDFLTGKNWLG"
     gene            complement(4446..4988)
                     /locus_tag="PKW44_03320"
     CDS             complement(4446..4988)
                     /locus_tag="PKW44_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
                     /protein_id="HQR50649.1"
                     /translation="MDARRVRLFLKRGGVLAYPTESCYGLGCDPRNATAVKCLLRLKR
                     RGAAKGLILIAAHPRQLRPFLAALSPALARRMHAAWPGPHTWLAPASRDCPAWLRGGH
                     DAVAVRVTAHGGAARLCELAGMALVSTSANLSGGKPAKTAAECRRLFGRRVLVMPGHI
                     GVRRRPSTIQDLASGAFIRR"
     gene            complement(4996..6264)
                     /gene="purD"
                     /locus_tag="PKW44_03325"
     CDS             complement(4996..6264)
                     /gene="purD"
                     /locus_tag="PKW44_03325"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HQR50650.1"
                     /translation="MKILVIGNGGREHALAWKLAQSPRATRVYVAPGNAGTALDPDMV
                     NVPITAIPELVKFAQDEQIALTIVGPEAPLSQGVVNAFIAAGLKIFGPTKAAAQLESS
                     KDFAKAFMQRHGIPTAAYATFSAAATAHDYVKAQGAPIVIKADGLAAGKGVVVAMTEA
                     EAHDAIDFMLSDNKLGDAGAKVVIEEFLQGEEASFIVMCDGEHILPLATSQDHKRLLD
                     GDQGPNTGGMGAYSPAPVVTPEIYAKVMREIIRPTIRGMARDGIRYTGFLYAGLMIDK
                     NGNPKTLEFNCRMGDPETQPIMLRLKSDLVTLVEHAVNGTLDQAEAEWDRRTALGVVM
                     ASAGYPDTPRKGDVISGLPKDLQGAHVFHAGTTLDNGLPVTSGGRVLCVTALGDTVRQ
                     AQSRAYQIAENIHFDGSQMRRDIGYRAIKG"
     gene            complement(6364..7950)
                     /gene="purH"
                     /locus_tag="PKW44_03330"
     CDS             complement(6364..7950)
                     /gene="purH"
                     /locus_tag="PKW44_03330"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HQR50651.1"
                     /translation="MTTIKRALISVSDKAGILEFAQVLRQFNVEILSTGGTAKLFRDH
                     NIPVIEVSDYTGFPEMLDGRVKTLHPKVHGGILGRRDLPEHVGAMQQAGIPDIDMVVV
                     NLYPFEATVAKPGCTLEEAIENIDIGGPAMVRSAAKNWNDVAVLTDAAQYADVAAEMK
                     SSGGAVGQATRFSLSVAAFNRISNYDAAISDYLSSFNADGSRNPFPAQTNGRFVKVQD
                     LRYGENPHQQAAFYRDLYPAPGSLVTAQQLQGKELSYNNIADADAAWECVKSFDSTAC
                     VIVKHANPCGVALGATVLEAYSKAFKTDSTSAFGGIIAFNVTVDGATAEAVSKQFVEV
                     LIAPDFSDDALAIFKVKANVRVLKIALPKGGNSAWEKGRNSHDSKRVGSGLLIQTADN
                     HEIMLADLKIVTRKQPTPQQMQDLLFAWRVAKYVKSNAIVFCGDGMTLGVGAGQMSRV
                     DSTRIAAIKAGNAGLPLTGSAVASDAFFPFRDGVDVLAEAGAACVIQPGGSVRDEEVI
                     AAANEHGLAMVLTGVRHFRH"
     gene            complement(7981..8214)
                     /locus_tag="PKW44_03335"
     CDS             complement(7981..8214)
                     /locus_tag="PKW44_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HQR50652.1"
                     /translation="MSDNDISSCVRGALEIYFRDLDGEPPHAVYDMLLACVEKPMLEY
                     VLNHANGNQSKAAEILGLNRNTLRKKLKQYNMQ"
     gene            complement(8214..9230)
                     /gene="dusB"
                     /locus_tag="PKW44_03340"
     CDS             complement(8214..9230)
                     /gene="dusB"
                     /locus_tag="PKW44_03340"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HQR50653.1"
                     /translation="MQIGPYQLKNNLIVAPMAGVTDRPFRQLCKRLGAGMAVSEMVTS
                     NSLLYGSEKTRRRANHEGEAEPKSVQIAGADPRMLAEAARYNVDNGAQIIDINMGCPA
                     KKICNVMAGSALLRDEPLVARILEAVVKSVPEVPVTLKTRLGWDRDHLNVPLVARIAE
                     QSGIQALAIHGRTRCQGYSGDAQYDLIAEVKQAIRIPVIANGDITTPEKAKYVLDKTG
                     VDGIMIGRAAQGRPWIFREIEHYLSTGAHRAPPQVTEIHGVLLDHLDDLYGFYGDLTG
                     LRVARKHISWYTKGLVGSAHFRHAMNQLQTVKEQLSAVNDFFGHLQQYGQHLQYDEPE
                     ALAA"
     gene            9413..11230
                     /gene="edd"
                     /locus_tag="PKW44_03345"
     CDS             9413..11230
                     /gene="edd"
                     /locus_tag="PKW44_03345"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019922789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HQR50654.1"
                     /translation="MKLNPTVEKVTERIRERSAETRQSYLQRLDAMAQRPRGVDRMGC
                     ANVAHSFAAMPADDKFKVVAERAPNIGVITAYNDMLSAHAPYQHFPDIIKAEAHKHGA
                     TVQVAGGVPAMCDGITQGMAGMELSLFSRDVIAMSTAVGLSHDVFDAALMLGVCDKIV
                     PGLLIGALHFGHLPTVFVPAGPMTTGLSNSEKSHMRVLAAEGKVGRKELLETESKAYH
                     GQGTCTFYGTANSNQMLLEAMGLHVPGAAFITPGTGLREELTREALRTVLDIVNGKSF
                     TPIGRMVDERCIVNAMAALMATGGSTNHLIHWVAVARAAGIIIDWNDFCTLSDVVPLV
                     ARVYPNGEADVNAFQAAGGPGYVIRELLDAGLMHGDVLTVRPQGIREFTGIPALQDGA
                     LHWQTAATSGDDEVLRPASAPFGPNGGIKVLRGNLGRSVIKVSAVPEECHIIEAPARV
                     FDSQEDLQKAFAAGELDCDVVCVVRWQGPHANGMPELHKLTPPLKALQNKGFDVALVT
                     DGRMSGASGKVPAAIHISPEAALGGALAKVRDGDVIRLNACSGELNVLISEEDWAARQ
                     PEKMPHSAREAHSIGMGRELFGGMRNNVTTAEEGACTWL"
     gene            11221..>11487
                     /locus_tag="PKW44_03350"
     CDS             11221..>11487
                     /locus_tag="PKW44_03350"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF013263.2"
                     /note="catalyzes the formation of pyruvate and glyoxylate
                     from 4-hydroxy-2-oxoglutarate; or pyruvate and
                     D-glyceraldehyde 3-phosphate from
                     2-dehydro-3-deoxy-D-glyconate 6-phosphate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="keto-deoxy-phosphogluconate aldolase"
                     /protein_id="HQR50655.1"
                     /translation="MAVNEKLTSLEVMMDAPVIPVIVLNDVVNAVPMAKALVAGGLRM
                     LEITLRTSVALECMRRIANEVPEAVVGAGTTDSVPVARASFRARA"
BASE COUNT         2170 a         3602 c         3523 g         2192 t
ORIGIN      
        1 cgaagccgac cacatccacc aggagttgct ggatcaaggc atcgtgctgg aagacacgcc
       61 gcaaggcacg atctggcggc gccaatgatc tacgaagcgc tacgccccct gctgttccgc
      121 atcgacgccg aacgcgccca ccacctgacg ctgcaagcgt tgcaagcttc ttatgacctt
      181 ggcttatccc gctggctgtt tcctgcgccg ccaccctgcc gtccgcgaca ggtgatggga
      241 ctgacgttcc ccaacccggt cggcctcgcc gccgggatgg acaagaacgg cgcccacgtc
      301 gacgcgctgg cagcgtttgg cttcggcttc atcgaggtcg gcaccgtcac gcctcggccg
      361 cagccgggca atcccaagcc acgcctgttc cgcattccgc aggcccgcgg catcatcaac
      421 cgcttcggct tcaacaacga tggcgtggat gcgctggtgg agaatgtcag aaaggcgcgt
      481 tatcgcggca ttctcggcat caacatcggc aagaatttcg acaccccgat cgaaggcgcg
      541 accgaggact acctgatctg cctgcgcaag gtctacgccc acgccagcta cgtcgccgtc
      601 aacatctctt cacctaacac caggaacctg cgccagttgc aggataaaga ggcattacaa
      661 gggctgctcg ccgcgctcaa gcaggaacag gccaaactcg ccgacgtcca cggcaagtat
      721 gtccccatcg cactgaaaat cgcacctgac ctggaaaccg cgcagatcgt ggaaatcgcc
      781 gacctgttgc tggcacacaa aatggatgcg gtgatcgcca ccaacactac gctctcgcgg
      841 gaaggcgtgg aaagcctgcc caacgcgcag gaagccggag ggctatcggg cgtgccggta
      901 cggctgaaat ccaccgccgt catcaagcag ctcagtctgg cgctgggcaa ggacatcccg
      961 atcatcgggg cgggcgggat tctcgaaggg ctggatgccg aggaaaagat caaggctggc
     1021 gccagcctgg tgcaggtcta cagcggcctg gtctatcgcg gccaggcgct ggtgccggaa
     1081 atctgccggg cgctgggcta accctcaacc agaaagcccg atcaatcccg tcccgaacgc
     1141 cagatcgaaa taatcagcca gatgccgaac agcgaggcga ggaagaagcc gaggaagccg
     1201 aacagcacgg tgcccccccg aactgtcatg acaatggagg agccgatgat gagcgccgag
     1261 gtaataatgc ccagcgtcag ccggttagtg ctctggtcga gctgatggcc gaagtggtcg
     1321 agacgcttga ggtcgagatc gatcttgacg cggccgcggc gcgcctctct cagcaaacgg
     1381 gaaagatcgc gtggcaaatc ggtggccagc gacagcagac cggaaacatt gcgccagccg
     1441 cgcttggcga tcgccttcgg catgtagcgc tcgacgatga tctttttcac gaacggcgtc
     1501 aggtggtgaa cgatctgaaa atccgggtcg agctgccgtc ccagcccctc cagcgtaatc
     1561 agcgccttga acagcatggt taaatccggc ggcaccacca ggtggttctc gcgcatcacc
     1621 gtagtcagat cgttgagcag cacgctgaac ctgacatgct tcaagggcgc gctttcgtag
     1681 ttgaagatca gctcgtcgac atcggcggac agcttgtcgt catcgatcgg cgcatccccc
     1741 gcccaagcaa tcaggatatc gcgcagcgca tgaatgtcct tggccaccag cgccgccagc
     1801 aggtcggcga tctggtcgcg tcgctcggtg ctgatacgtc ccaccatgcc gcagtcaatg
     1861 atggcgagcc ggttgcccgg cagatagaac acgtttccgg ggtgcggatc ggcgtggaaa
     1921 aaaccgtcta ccagcaccat cttgagcacg gcgtcggcac cgcgcgcacc cagtatcttg
     1981 aggttgagcc cggatagcag tgcgctgtcg agatcgttgc cgggaatgcc gtcgatatac
     2041 tccatcacca acagatgttc gctggtatgc tcccagtgca ccttggcgaa gacaacgtca
     2101 gggtcgtcgg caaaattgtg agcgaagcgg tcggtattgc gcgcctccat cgccagatcc
     2161 agttcgcgtc gcagcgactt ggcgaactgc ccgacaatct tgcccggctg gaagcgtctc
     2221 gactctggca tttccgattc cagcagccgc gccagatggg acagcaggcg caaatccgca
     2281 tcgatgcggc cttgcacatt ggggcggcgc accttgagca ccaccttggt accgtcctgc
     2341 agttttgcca ggtgcacctg tgcgatggag gcgccggcaa tggcctcggt ttgcagctcc
     2401 tggaatacct cgaacggcga gcgtcccagc gccttttcca gctccggcag cagcgcttcg
     2461 aacggttccg gcggcacctt gtcctgcaat tttgaaaatt cggcaatcca gtccggcgga
     2521 aaaatatccg gccgggtcgc cagcacctga ccgagcttga cgaaggtggg tcccagctcc
     2581 tggatcgcca gccgcacccg caccgccggc tccaggtgca tgatctcctc ggactggtgg
     2641 cggttgagca tgttgccggc acgctcggtg atgttgccca gccccagctg cttgacgaaa
     2701 tcgcccaagc cgtaatggat catgatgccg gcgatttcgc gcatgcggcc gaaatcgcgg
     2761 atgacggaga tggtttcgcg gatcatgggc ggagagttgg gaggcctcgt ttatttttgc
     2821 ttgagccgct cagccttgtc ggcgatgcgg gtggcgattt cggacagctt gcggctggcg
     2881 agcgccgcca cgcgcgcacc gacttcgcgg gcggcgttct tgccggcgtc cccggcctgc
     2941 tgtgcggtag cccgcagacg attgcccaga tcttccgcca ccgccttgac ccgctcgccg
     3001 gtgccggtgc cggcatgtct ggcatgttcg gccagcgctg ccatctcctg cttcaccaga
     3061 ccactggcgc cctgccctac acggctcact gtttccagaa atacctgttc cagattcttc
     3121 agttcgctga tgccctgctt caggtcctgt tcggcgaatt cctgggcgtg tgaggacacc
     3181 tcgccccaag ccagttgcat cgcctccgcc gcgtggccga gggcgtcatc catcccattc
     3241 agcgcatccc ccagcgccac gcgcacatct tccgcacgct cggccgcacc cagactgacg
     3301 ccctcggtaa tcgcgccgat gacctcccgg atttcctgtt cggccagttg gcgctgggtc
     3361 agcgccttga ctgtgagctc atgcagcttt tcccggacgt tctcgccgct acgcagcaag
     3421 ttacgcgcac tgtccttgat ttgctccagt gaaaattcct ttttgtcgct catggcatgc
     3481 ctcctgttat ttagcgtact ttcttaattt tacacctctg gcgcgctgca agcgctggcc
     3541 atttagccca gccagttctt gccggtcaga aaatccgtat acagcttcgc ttctgcggat
     3601 cccgcttccg gtcgccagtc gtagcgccat ttgacgatgg ggggcagcga catcaggatg
     3661 gattcagtgc ggccgccgga ttgtaggccg aacaacgtgc cgcggtcgta caccaggttg
     3721 aattcaacat agcgcccgcg gcgataggcc tggaagtcac gttcgcgctc gccgtattcc
     3781 agatccttgc gacgttgcag aataggcaga taggcgtgca ggaaagcgtc gcccacgctt
     3841 ttcatcatgg cgaagctccg ttcgaagccc agctcgttga agtcgtcgaa gaacacgcca
     3901 ccgatgccgc gcggctcctt gcggtgcttg agggtgaagt agtcgtcgca ccattgcttg
     3961 aagcgtgggt aaagctcggc gccgtggggg tcgagcgcca gcttgcaggt gcggtggaaa
     4021 tgtaccgcgt cttcctcgaa gccataatag ggcgtcaagt ccatgccgcc accgaaccac
     4081 cataccggcg cctcgccggc ttttttcgcc atgaagcaac gcacgttcaa gtgcactgtc
     4141 ggcgcatacg gattgcgcgg gtgcagcacc agcgatacgc cggcagcctc gaagccgcgt
     4201 ccggccagct ccggccgccc cgcgctggcg gagggcggca gcttgtcacc catgacgtgc
     4261 gagaaattga ctccgccgcg ctccagcacg ttgccctcct caagcacgca acttaccccg
     4321 ccgccacctt ccgggcgctg ccacgagtcg tgcaggaatt gcttgccctc agcgacctgc
     4381 agcgcctcga cgatgcggtt ttgcaggtcg gtgaagtaat cttttacggc atcgaaatcc
     4441 atgtcctacc ttctgatgaa agccccgctg gcgaggtcct gaatagtgga gggacggcgg
     4501 cgcacgccga tgtgtccggg catgaccagc acgcgcctgc cgaacaggcg gcggcattcg
     4561 gcagcagttt tggccggctt tccgccgctc aggttggcgc tggtagaaac gagcgccatt
     4621 ccggccagtt cacaaaggcg tgcggccccg ccatgggcgg tgacccgtac cgcaacggcg
     4681 tcatgaccgc cgcgcagcca ggccgggcaa tcacgcgagg ctggggccag ccaggtatgc
     4741 ggaccaggcc aggcggcatg catgcgccgc gccagggcag gtgacaatgc ggccaggaaa
     4801 gggcgcagct ggcgtggatg ggcggcaatc aggatcagcc ccttggccgc accacgccgc
     4861 ttcagccgca gcaagcactt taccgccgtg gcgttgcgcg ggtcgcagcc caagccatag
     4921 caggattcgg tcgggtaggc gaggacgccg ccgcgcttca ggaacagacg aacgcgccgc
     4981 gcgtccaccc gtgcctcatc ccttgatggc gcgatagcca atgtcgcgcc gcatctggct
     5041 gccgtcgaaa tggatgtttt ccgcgatctg gtaggcgcgg ctttgcgcct gcctgacggt
     5101 atcgcccaac gcggtgacac acagcacgcg accgccgctg gtgaccggca ggccgttatc
     5161 gagtgtggtg ccggcatgaa atacgtgggc accctgcaag tccttgggca ggccgctgat
     5221 gacgtcaccc ttgcgcgggg tgtcgggata gccggccgac gccatgacca cgcccagcgc
     5281 ggtgcggcgg tcccattcag cttccgcctg gtccaacgta ccgttgacgg cgtgctccac
     5341 cagcgttacc aggtcgctct tcagccgcag catgatcggc tgggtttccg ggtcgcccat
     5401 gcggcagttg aattccagcg tcttcgggtt gccattttta tctatcatca gcccagcata
     5461 aaggaagccg gtgtagcgga tgccgtccct ggccatgccc cgaatggtgg ggcggatgat
     5521 ttcgcgcatg actttggcgt aaatttccgg ggtgaccaca ggtgccggcg agtaggcgcc
     5581 catgccgcca gtgttcggtc cctggtcgcc gtccagcagg cgtttgtggt cctgactggt
     5641 ggccagcggc agaatgtgtt caccatcaca catgacgatg aagctggctt cctcgccttg
     5701 caggaactcc tcgatcacga ctttggcgcc ggcatcaccc agcttgttgt cagaaagcat
     5761 gaaatcgatc gcgtcatgcg cctcggcttc ggtcatggcg acgaccaccc ccttgccggc
     5821 ggccaagccg tcggctttga tgacgatagg cgcaccctgt gcttttacat agtcatgcgc
     5881 ggtagcggca gcggagaatg tcgcgtatgc agccgtcggg ataccgtggc gctgcatgaa
     5941 tgccttggca aaatccttgg aagattccag ttgcgcggca gccttggtcg gcccgaaaat
     6001 cttcagtccg gcggcaatga aagcgttgac caccccctgc gacagcggcg cctcggggcc
     6061 gactatggtc agggcaatct gctcgtcctg cgcaaacttg accagttccg gaattgccgt
     6121 aatcggcacg ttgaccatgt ccggatcaag tgcggtaccg gcattgccag gcgccacata
     6181 gacacgcgtc gccctcgggc tttgcgccag tttccaggcc agcgcatgtt cgcgcccgcc
     6241 gttgccgatg acaaggatct tcatgtaatg cttcccttga aacagggaag gacaaaaggg
     6301 gagaggggaa aggtaaaagc gccgaagctc acccttctcc cttccccctt cccccttccc
     6361 gggttaatgc ctgaaatgcc gcacgccggt cagcaccatg gccagaccgt gttcgttcgc
     6421 cgcggcgatg acttcctcgt cacgcacgct gccgccgggc tggatgacgc aggcggcgcc
     6481 agcttcggcc agcacgtcca cgccatcgcg gaacgggaag aaagcatcgg aagccacggc
     6541 tgagccagtg aggggcaacc ccgcattacc cgccttgatc gcggcaatac gggtgctgtc
     6601 cacacgactc atctggccgg caccgacccc cagcgtcatg ccgtcgccgc agaacacgat
     6661 ggcgttggat ttgacgtact tggccacgcg ccaggcgaac agcagatcct gcatttgttg
     6721 tggcgtcggc tgcttccggg taacgatttt caggtcggca agcatgattt cgtgattgtc
     6781 cgcggtctga atcagcagcc cggagccgac acgcttgcta tcatgcgaat tgcggccttt
     6841 ttcccacgcg ctgttgccgc cttttggcaa cgcgatcttg aggacgcgca cattggcttt
     6901 caccttgaaa atcgccagcg cgtcatcgct gaagtccggc gcgatcagca cttcgacaaa
     6961 ttgcttggaa actgcttctg ccgtcgcacc gtccacagtc acgttgaacg cgataatgcc
     7021 gccgaacgcc gaggtcgagt cggtcttgaa tgccttgctg taggcttcca gcacggttgc
     7081 gcccagcgcc acgccgcacg ggttggcatg tttgacgatg acgcaggcgg tgctgtcgaa
     7141 ggatttgaca cattcccagg cggcatcggc atcggcaatg ttgttgtagg acagttcctt
     7201 gccttgcagt tgctgtgccg tcaccagcga accgggcgcg gggtacaggt cgcggtagaa
     7261 cgcggcttgc tggtgcgggt tttcgccgta gcgtaagtcc tgcaccttga cgaaacggcc
     7321 attggtctgc gcagggaaag ggttgcgcga accatcggca ttgaaggagg aaagataatc
     7381 gctgatcgcc gcatcgtaat tgctgatgcg attgaaggcg gcgaccgaca gggaaaagcg
     7441 ggtggcttgg ccgacggcgc cgcccgaact cttcatttcc gcggcgacat cggcatactg
     7501 cgcggcgtcg gtcagcactg ccacatcgtt ccagttcttg gcggcggacc gcaccatggc
     7561 cggaccgccg atgtcgatgt tctcgatggc ctcttccagc gtgcagccgg gcttggccac
     7621 cgtggcttca aaagggtaga ggttgaccac taccatgtcg atatccggga tgcctgcctg
     7681 ctgcatggcg ccgacatgtt ccggcaggtc gcgccggccg aggataccgc catggacttt
     7741 gggatgcagt gtcttgacgc ggccatccag catttccggg aaaccggtgt agtcactgac
     7801 ttcaatgact ggaatattgt ggtcgcggaa caacttggcg gtgccgccag tggaaagaat
     7861 ttcgacgttg aattggcgca ggacttgggc gaattcaaga atgccggctt tgtcagaaac
     7921 gctgatgagc gcccgtttga tagtagtcat ggtgagtcag ttgaatgaaa agtgagaaaa
     7981 ctactgcatg ttgtattgct tgagtttctt gcgcagggta ttgcggttga ggccaaggat
     8041 ttcggcggct ttgctctggt tgccgttggc gtggttcagc acgtattcca gcatgggctt
     8101 ttccacgcat gccagcagca tgtcgtatac cgcatgcggc ggctcaccgt cgagatccct
     8161 gaagtagatt tccagcgcgc cgcgcacaca ggacgaaata tcattgtcgc tcatcaggcc
     8221 gccagcgcct ccggttcgtc gtattgcaag tgttggccgt actgctgcag atggccgaag
     8281 aaatcgttga ccgcgctcaa ctgttccttg acggtctgca gctgattcat ggcatggcgg
     8341 aaatgggcgg agccaaccag gcccttggtg taccacgaaa tgtgcttgcg cgccacgcgc
     8401 agaccggtga ggtcgccata gaagccgtac aggtcatcca gatggtcaag cagcacgccg
     8461 tggatttccg tcacttgcgg cggcgcccta tgggcaccgg tactcaggta atgttcgatt
     8521 tcgcggaaaa tccacgggcg gccctgcgcc gcgcggccga tcatgatccc atccacgccg
     8581 gtcttgtcca gtacgtactt ggctttttcc ggagtggtga tgtcaccgtt ggcgatcacc
     8641 gggatgcgaa tcgcctgctt cacttcggca atcaaatcgt attgggcgtc accgctgtaa
     8701 ccttggcagc gcgtgcggcc gtggatagcc agcgcctgga tcccggactg ttcggcgatg
     8761 cgggcgacca gcggcacatt aaggtggtcg cggtcccagc ccaggcgggt cttcagcgtc
     8821 accggaacct cgggaacgga tttgaccact gcttcgagga tgcgcgccac cagcggctca
     8881 tcgcgcaaca gggcggagcc cgccatgaca ttgcagatct ttttggccgg acagcccatg
     8941 ttgatgtcga tgatctgcgc accgttgtcc acgttgtagc gcgcggcttc agccagcatc
     9001 ctggggtcgg cgccggcgat ctgcaccgac ttcggctcgg cttcgccctc gtggttggcg
     9061 cggcggcggg tcttctcgct gccatacagc agcgagttgg aagtcaccat ctcgctcacc
     9121 gccatgcccg cgcccagccg cttgcacaac tggcggaaag ggcggtccgt cacgcccgcc
     9181 atgggggcga cgatcaggtt gttcttcaac tggtaggggc cgatttgcaa gatttttctt
     9241 gtaccgagca agcgggaaag ctgcacattt tatatgcaaa gccgcggcag gcaaaggcaa
     9301 aattgcaggt tgttttgcgc gttccggcaa acccggccgc ggggcagaat tgggtttttg
     9361 caccaaattg ggttacatta ctggtttggt ctaacttgtt gaattcatcg aaatgaaact
     9421 gaatcctacc gttgaaaaag tcacggaacg catccgtgaa cgcagcgcgg aaacccgcca
     9481 aagctacctg cagcgactcg acgccatggc acagcgcccg cgcggcgtag atcgcatggg
     9541 ctgtgccaat gttgcgcact ccttcgccgc catgcctgct gacgacaaat tcaaggtggt
     9601 ggccgaacgc gcacccaaca tcggcgtcat caccgcctac aacgacatgc tgtcggcgca
     9661 cgccccttac cagcacttcc cggacatcat caaggccgaa gcacacaagc acggcgccac
     9721 cgtgcaggta gcgggcggcg tgccggcgat gtgcgacggc atcacccagg gcatggccgg
     9781 catggagctg tccctgttca gccgcgatgt gatcgccatg tccacagcgg tagggctcag
     9841 ccatgacgtg ttcgacgccg ccctgatgct gggcgtgtgc gacaagatcg tgccggggct
     9901 gctgatcggc gccttgcatt tcggccatct gcccaccgtg ttcgtgccgg ccgggccgat
     9961 gaccaccggc ctctccaaca gcgaaaaatc gcacatgcgc gtgctggcgg cggaaggcaa
    10021 ggtcggccgc aaggaactgc tggaaaccga atccaaggcc taccacggcc aaggcacctg
    10081 caccttctac ggtaccgcca acagcaacca gatgctgctg gaagcgatgg gcctgcacgt
    10141 gccgggcgct gccttcatca cccccggaac cgggttgcgc gaggagctga cacgcgaggc
    10201 gctacgcacc gtgctggaca ttgtcaacgg caagtccttc acccccatcg gacgcatggt
    10261 ggacgagcga tgtatcgtca acgccatggc agcactcatg gcgaccggtg gttccaccaa
    10321 ccacctgatt cactgggtgg ccgtggcgcg cgccgcaggc atcatcatcg actggaacga
    10381 tttctgtacc ctgtccgacg tggtgccgct ggttgccagg gtctacccca acggcgaagc
    10441 cgacgtcaac gctttccagg cagcgggcgg ccccggctac gtgatccgcg aactgctgga
    10501 tgccggcctg atgcacggag acgtgctgac ggtacgtccg cagggcatcc gcgaattcac
    10561 cggcatcccg gcgctgcaag acggcgcgct gcactggcag acagcggcca cctccggcga
    10621 cgacgaggtg ctgcgaccgg cttccgcccc gttcggcccc aatggcggca tcaaggtatt
    10681 gcgcggcaac ctgggacgca gcgtaatcaa ggtttccgcc gttccggagg aatgccacat
    10741 catcgaggcg ccggcgcgtg tgttcgattc gcaggaagat ttgcaaaaag cctttgccgc
    10801 aggcgaactg gactgcgacg tggtctgcgt ggtgcgctgg caaggtccgc acgccaacgg
    10861 catgccggaa ctgcacaagc tgaccccgcc gctgaaggcg ctgcagaaca agggttttga
    10921 cgtagcgctg gtgaccgatg gccgcatgag cggcgcttcc ggcaaggtgc cggccgccat
    10981 ccacatctcg ccggaagccg ccttgggcgg ggcgctggcc aaggtccgcg acggcgatgt
    11041 aatccgcctc aatgcctgct ccggcgaact caacgtgctg atttctgaag aagactgggc
    11101 tgcccggcaa ccggaaaaaa tgccgcatag cgcgcgcgaa gcacattcca tcggcatggg
    11161 acgagagctg tttggcggca tgagaaacaa tgtaacaacg gctgaagaag gagcttgcac
    11221 gtggctgtaa atgagaaact gacatcgctc gaggtcatga tggacgcgcc ggtgatcccg
    11281 gtgatcgtac tcaacgacgt ggtgaacgcc gtgcccatgg ccaaggcgct ggtggccggc
    11341 ggcctgcgca tgctggaaat caccctgcgc acttccgtgg cactggaatg catgcggcgc
    11401 atcgccaacg aagtgccgga agccgtggtc ggcgccggca ccaccgacag cgtacctgtt
    11461 gcccgggcgt catttcgcgc ccgtgcc
//
LOCUS       DAOMFA010000040        11297 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_585_length_11297_cov_5.086017, whole genome shotgun sequence.
ACCESSION   DAOMFA010000040 DAOMFA010000000
VERSION     DAOMFA010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 11297)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11297)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11297
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_585_length_11297_cov_5.086017"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            109..504
                     /locus_tag="PKW44_03355"
     CDS             109..504
                     /locus_tag="PKW44_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HQR50656.1"
                     /translation="MSVLNTAEKQQVMTELSQRRAQLTEEIRIELERAGHQHFADLAG
                     EVADVGDASVADALVDQDIAIVRRQVAELTQVEAAQKRVAGADFGECDECGAAIGVPR
                     LLIVPHATRCVACQEQHDKMYAHESTPKM"
     gene            509..1672
                     /locus_tag="PKW44_03360"
     CDS             509..1672
                     /locus_tag="PKW44_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HQR50657.1"
                     /translation="MSHHQFFATCPRGLEALLADELSALGAQNVKSTDGGVGFAGDLL
                     LAYRANLESRVATRILMQVAYGRYQNEDHLYQAAYALPWADWFEVGRTFMVKVTAVKC
                     PLKSLEFVTLRIKDAVCDQFRGKVGSRPYIDTRQPDVRIHAFLTTDEYHLYLDTSGNA
                     LYQRGLRRLSVEAPLRENLAAGILKLTGWQPGTPLYDPMCGSGTFLLEAAMMALDIAP
                     GSGRSFGFEKLNSFDADAWKAMRQQAQARAKPVEFQHIYGSDADLRAVRVSKQNLEQA
                     DLLEAVWLTQKDITKVEAPADHGVLIANPPYGVRIGEQEELAALYPKIGETLKRNFAG
                     WNAYFLTSDLRLPKLMRLAPSKKTPLFNGPLECRLFEIKVVAGSNRKPEKGER"
     gene            1669..2055
                     /locus_tag="PKW44_03365"
     CDS             1669..2055
                     /locus_tag="PKW44_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HQR50658.1"
                     /translation="MKEEGQDRLDALRERLRSFVRERDWEQFHSPKNLAMAMIVEAAE
                     LVEHFQWATEQESHELGPEKRAKVEQEIADTFIYLLRLSDVLGVDLIEAAHRKMDLNA
                     EKYPAEKVRGRNDKYTEYQSSEPDSR"
     gene            2070..3083
                     /locus_tag="PKW44_03370"
     CDS             2070..3083
                     /locus_tag="PKW44_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019897310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HQR50659.1"
                     /translation="MDQVRKLRWGILGAARVNERLLPAIVEAPNAQLVAIASRRPGAA
                     GETLRKYAPRQQGVREHDQPEALLDNPEVEAVYLPMANEEHAEWALRAIRRGKHVLIE
                     KPMALTVADIEAIEAAAKEHRVAVMEGFMYRFHPQHARVQEIVAASLIGEVRSVRASY
                     SFMMRPARMYRLARDPGQGGGAMWDIGPYAIHSLRWCFGSEPVSVAAMAKYIESGADS
                     AASGVLDFGDGRFGHFDISFERARRSEYEIIGTKGGVKCHAAWQLPGDVPVISWWTED
                     GRECMEHYPAANHFNLEIEHFSDCVLHGKVPLLTLEDAKANCRAIVATLQSAAQGRVV
                     KLD"
     gene            3124..4506
                     /gene="dnaB"
                     /locus_tag="PKW44_03375"
     CDS             3124..4506
                     /gene="dnaB"
                     /locus_tag="PKW44_03375"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HQR50660.1"
                     /translation="MADDSLASLKLPPHSVEAEQSVLGGLLLENDALDRIADILTADD
                     FYRHDHRVIYEHISRLIEHSKPADIVTLAEALESTAQLAGVGGIAYLGALAQNTPSAA
                     NIRRYAEIVRGRAVMRQLVQVGSGIAESALSPQGKDVAQLLDEAEARIFEIAEGGHRS
                     SQGFIDIKTILPQVADRIDQLFQRDNASDVTGIPTGFTDLDSMTSGLQPGDLVIVAGR
                     PSMGKTAFSLNIAENVALDTGLPVAVFSMEMAATQLAMRMIGSVGRLDQHKMRTGRLE
                     DEDWVRLTTALGKLNDAPLFIDESAGLNALEVRARARRLHRQCGKLGLIVLDYLQLMS
                     ATRSGENRATEISEISRSLKALAKELDVPVVALSQLNRGLEQRPNKRPVMSDLRESGA
                     IEQDADVILFIYRDEVYNPDSQDKGTAEIIIGKQRNGPIGTVRLTFLGEHTRFENFAS
                     TGGYHGGSEY"
     gene            4530..5621
                     /gene="alr"
                     /locus_tag="PKW44_03380"
     CDS             4530..5621
                     /gene="alr"
                     /locus_tag="PKW44_03380"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018410579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HQR50661.1"
                     /translation="MSRPIYATIHLDALRHNLTVAKRHAPDSSIMAVIKANGYGHGLL
                     RVAHSLDAAEGFAVLGVEEGVALREAGFRQTILLLEGLFEAAELPVAAQHGFTVVVHS
                     GPQLLMLEAARNVPAVDVFLKVNTGMNRLGVPVGRFWSFYDRLRACKTAGKLTLMTHF
                     ATADEPAGVAEQFSLLDDLTREIAMPRSMANSAATLRYPHTHADWVRPGIMLYGATPF
                     ADQSAAELGLKPAMSLSSQVIAVQNLQAGQSVGYGHRFTAERQTRVGVVACGYADGYP
                     RHAGQYPAGENQRGAPIVVAGQLTRTIGRVSMDMLYADLTNIPQADIGSPVELWGAQV
                     PVDDVATASGTVGYELLCALAPRVPVVGV"
     gene            5621..6979
                     /gene="radA"
                     /locus_tag="PKW44_03385"
     CDS             5621..6979
                     /gene="radA"
                     /locus_tag="PKW44_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HQR50662.1"
                     /translation="MAKQKSVYTCTECGGQTPKWQGQCPSCNAWNTLVETIAEAAPAN
                     RYAALAPAAGLQKLSEVEAAEVPRQPTGIMEFDRVLGGGLVPGGVVLIGGDPGIGKST
                     LLLQTLCHIGDQRKVVYVSGEESAQQIAMRAKRLSLDASPLQLLAEINLEKILSTLQA
                     HQPDVAVIDSIQTVYSEALQSAPGSVAQVRECSAQLTRLAKQLGINVILVGHVTKEGS
                     LAGPRVLEHIVDTVLYFEGDPNSSFRLIRAFKNRFGAVNELGVFAMTEKGLREVSNPS
                     ALFLSHHAEQVAGSCITVTQEGTRPLLVEVQALVDEAHAPNPKRLCVGLEQNRLAMLL
                     AVLHRHAGVACFDQDVFVNAVGGVRIAEPAADLAVLLAIVSSLKNKQLHNKLIVFGEV
                     GLAGEVRPVQRGQERLKEAAKLGFTQAIVPKANLPKQPIKGIEVFGAERLEQALARLR
                     EA"
     gene            complement(6976..7728)
                     /locus_tag="PKW44_03390"
     CDS             complement(6976..7728)
                     /locus_tag="PKW44_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HQR50663.1"
                     /translation="MNDYQPPHQHLKDRVILVTGAGQGIGKAAALAFAAQGATLILLG
                     RKVKKLEAVYDEIEAAGGPQPLIFPLDLEKAGEEEFKGLAEGIYQQIGRLDGILHNAS
                     RFDNLSPLEIQTQEQMLGMLRTNTVAPFLLTKACLPLLKRAPDASVIVTSTTAAHHPA
                     AFWGAHAISNGAAETLVKIWALELETFPKLRINALIPGPVQCPQRKKSHPGELHGKLP
                     QPEAIMPLYLYLMGHDSAGVSGQVFEAQKTTS"
     gene            complement(7776..8051)
                     /locus_tag="PKW44_03395"
     CDS             complement(7776..8051)
                     /locus_tag="PKW44_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018507677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HQR50664.1"
                     /translation="MPARRLRIHGLVQGVGYRESMWREAEKLGISGWVRNRRDGTVEA
                     LVCGPDDAISVIIAWARHGPRLSLVERVLIEEDEATVTASFTVIPTE"
     gene            complement(8056..8451)
                     /locus_tag="PKW44_03400"
     CDS             complement(8056..8451)
                     /locus_tag="PKW44_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HQR50665.1"
                     /translation="MNPQLYDFLLHWGIMSLSLWVASHLFSGLEFSSKSSLLVSALML
                     GFVNAILKPILILLTLPLTLLTLGLFLLVINALMIQLVAALVSGFKVSDFWTAFFASI
                     FIAIFGLFLEWLLPGNGLLAIPAGNLVSA"
     gene            complement(8448..9749)
                     /gene="hpnE"
                     /locus_tag="PKW44_03405"
     CDS             complement(8448..9749)
                     /gene="hpnE"
                     /locus_tag="PKW44_03405"
                     /EC_number="1.17.8.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxysqualene dehydroxylase HpnE"
                     /protein_id="HQR50666.1"
                     /translation="MSRVAVIGGGCAGLAAAAELASQGISVTLYEASRQLGGRARGLN
                     WKGKRLDNGQHILLGAYSETLRLLRLAGVDLSTALHRLPLQLIQHRQFRLRASTHLPA
                     PLHILAGLLRADGLSKRERLAALRFMASLKLSGFRLAQDEPLSAFLAGHRQPQKLLQW
                     LWEPICLAALNTPLDRASAQVFLNVLRDSFSRSKSDSDLLLPRCDLSLLLAEPLAEYI
                     RQHGGEVRLGSPATAILQEKDGFVVDNDGDRRQFSHVIAAVSPFRFGEVAAGLHQLQD
                     AAGICAGFDYQPIYTVYLQYPAATRLPFPMIGLTGGYAQWVLDRGALDEQHGLLAVVI
                     SAEGRHQSLTQEALAAEVTVELAQAFPDLPPPEWHKVIAEKRATFTCRPNLSRPAQRT
                     LLTNFYLAGDYTAGDYPATIEGAMRSGVKCAHLVLENIGNP"
     gene            complement(9746..10585)
                     /gene="hpnD"
                     /locus_tag="PKW44_03410"
     CDS             complement(9746..10585)
                     /gene="hpnD"
                     /locus_tag="PKW44_03410"
                     /EC_number="2.5.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008058803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016767 - geranylgeranyl-diphosphate
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="presqualene diphosphate synthase HpnD"
                     /protein_id="HQR50667.1"
                     /translation="MSDPHQYCQQKAAASGSSFYYSFLFLPKPKRQAITALYAFCREV
                     DDVADECSDSELARTKLNWWRGEIANLYAGKPTHPVTQALQFAIGEYGLAEEHFREII
                     DGMEMDLDQNRYRDFKELQLYCYRVASVVGLLSASIFGYRDRKTLKYAHDLGLAFQLT
                     NIVRDVGEDARRGRIYLPADELEQFGVPEADILQGRETVNLRQLLEFQIERAQGYYEK
                     AFAELPAADRKAQRTGLVMAAIYRTLLDEIRAGGCEKVLNQRISLTPLRKLWLAWKTW
                     VRN"
     gene            complement(10597..>11297)
                     /gene="hpnC"
                     /locus_tag="PKW44_03415"
     CDS             complement(10597..>11297)
                     /gene="hpnC"
                     /locus_tag="PKW44_03415"
                     /EC_number="2.5.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004311 - farnesyltranstransferase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="squalene synthase HpnC"
                     /protein_id="HQR50668.1"
                     /translation="DEGDLAPEQRLALLDGFRKELDRLAAGNPPETEFCHTLAAMIAT
                     HQLPLQPFYDLLDAFSQDVVKTRYENFGEVVDYCRRSANPIGRLLLHLYGEATPRNIG
                     YSDAICSALQIINFLQDVAIDYRKDRIYFPLDEMKKYRITEQQIARGDAGGMWWGFMQ
                     FEIERARKLLQSGAPLGLVLKGRIGLEMRTIIAGGETILRKLHKSHGDMFNQRPVLRS
                     TDWAYMLYRAVRAK"
BASE COUNT         2189 a         3418 c         3585 g         2105 t
ORIGIN      
        1 gccagactcc tgcaggaagc ctatcccatc gagtttctgg agtttgtcga gcggctgcgg
       61 gataataacc ccaacacctc caatcgtttg atcatttagg aagacatcat gtccgtattg
      121 aatacagccg aaaaacagca agtcatgacc gaactgtccc agcgtcgcgc ccagctcacc
      181 gaagaaatcc gcatcgaact ggaacgtgcc gggcaccagc acttcgccga cctggccggc
      241 gaagtggccg atgtcggcga cgcctctgtg gctgacgcct tggtcgatca ggatatcgcc
      301 atcgtgcgcc gtcaggtggc agagctgacc caggtcgagg ctgcacagaa gcgcgttgcc
      361 ggagccgact tcggcgaatg cgacgagtgc ggcgccgcaa ttggcgtgcc gcgtctgctg
      421 atcgtgcctc atgccacccg ctgtgtggct tgccaggaac agcacgacaa gatgtacgcc
      481 cacgaatcca ccccgaagat gtaagggcgt gagccatcat caattttttg ccacctgccc
      541 gcgcgggctg gaagccttgc tggcggacga actgtcagca ctgggcgcgc aaaatgtaaa
      601 gtctaccgac ggcggcgtcg gtttcgccgg cgatcttctg ctggcctacc gtgccaacct
      661 ggaaagccgc gttgccaccc gtatcctgat gcaggtggcc tacgggcgct accagaacga
      721 agatcatctg taccaagcgg cctacgcgct gccttgggcc gactggttcg aagtggggcg
      781 cactttcatg gtcaaggtga ctgccgtcaa gtgtccgctc aagagcctcg agttcgttac
      841 cctgcgcatc aaggacgcgg tgtgcgacca gttccgcggc aaggtcggca gccggcccta
      901 catcgacacc cgccagccgg acgtacgcat ccatgccttt ctcacgactg acgaatacca
      961 tctgtacctg gatacttccg gcaacgccct ctatcagcgc ggcctgcgcc gcctcagcgt
     1021 ggaagcgccg ctgcgcgaaa acctcgcggc aggcattctc aaattgaccg gctggcaacc
     1081 cggaacgccg ctgtacgacc cgatgtgcgg cagcggtacc tttttgctgg aagcggccat
     1141 gatggcgctg gacatcgccc ccggcagcgg ccgcagcttc ggcttcgaga agctcaacag
     1201 tttcgacgca gacgcttgga aagcgatgcg ccagcaggca caggcacggg ccaaaccggt
     1261 tgagtttcag cacatttacg gcagcgacgc cgacctgcgc gcggtgcgcg tctccaagca
     1321 gaacctggag caggccgacc tgctggaagc ggtgtggctg acgcagaagg acatcaccaa
     1381 ggtggaagcg ccggccgatc atggtgtgct gatcgccaac ccgccctatg gcgtgcgcat
     1441 cggtgaacag gaagaattgg ccgcgttgta tcccaaaatc ggcgagacgc tgaagcgcaa
     1501 cttcgctggc tggaatgctt atttcctcac ctctgatctg cgccttccca agctgatgcg
     1561 gttggcgccg tcgaaaaaga caccgctatt caacggcccg ctggaatgcc gtctttttga
     1621 aatcaaggtc gtggcgggca gtaaccgtaa acccgagaaa ggggaaaggt gaaggaggaa
     1681 gggcaggatc ggctggacgc gctgcgcgag cgcttgcgca gcttcgtgcg cgagcgcgac
     1741 tgggagcagt ttcattcgcc caagaatctg gcgatggcca tgattgtgga agctgccgaa
     1801 ctggtagagc acttccaatg ggcgaccgag caggagagcc acgaactggg cccggagaag
     1861 cgcgccaagg tggagcagga gattgccgac acgttcatct acctgctaag gttgtccgac
     1921 gtgctgggag tggacctgat cgaagccgca catcgaaaaa tggatctcaa cgccgagaaa
     1981 tatccagccg agaaagtgcg tggacgcaac gacaaatata cggaatacca gtcgtcagag
     2041 cctgatagtc gttgaaatag ggagacatca tggaccaggt cagaaaactc cgctggggca
     2101 tcctgggcgc tgcccgcgtc aacgagcgcc tgttgcctgc catcgtcgaa gcgcccaacg
     2161 cgcagttggt cgccattgcc agccggcgcc ccggggctgc cggcgagaca ttgcgcaaat
     2221 acgctccccg gcaacagggt gtgcgcgagc atgaccaacc ggaagccttg ctggacaatc
     2281 cggaagtcga ggccgtctac ttgccgatgg ccaatgagga gcatgccgag tgggcgctgc
     2341 gcgctatccg gcgaggaaag cacgtgctga tcgaaaagcc gatggcgctc accgtggccg
     2401 atatcgaagc catagaagcg gcagcaaagg aacaccgggt tgccgtgatg gaaggcttca
     2461 tgtaccgctt tcatccgcaa cacgcccgag tacaggagat cgtcgccgcc agtcttattg
     2521 gagaggtgcg ttcggtgcgt gccagctatt ccttcatgat gcgcccggca cgtatgtacc
     2581 gcctggctag ggaccccggc caagggggcg gcgccatgtg ggatatcggc ccttacgcca
     2641 tccactcgct gcgctggtgt tttggcagcg aaccggtttc tgtggcagcg atggcgaaat
     2701 acatcgaaag tggtgcagac agcgcggcca gcggcgtgct ggactttggc gacggccgct
     2761 ttggccactt cgatatcagc ttcgagcgcg cgcgtcgctc ggaatacgag attatcggca
     2821 ccaagggtgg cgtcaagtgt cacgccgcct ggcaattgcc gggtgatgtg ccggtcattt
     2881 cctggtggac agaagacggg cgcgaatgta tggagcacta cccggcggcc aaccacttca
     2941 acctggaaat cgagcacttc agcgactgcg tgctgcacgg caaggtgccg ctgctgacgc
     3001 tggaggatgc caaggccaat tgccgtgcca tcgttgccac gttgcagtcg gcagcgcagg
     3061 gcagggtggt gaaactggac taacagggcg cctagaaatt cccggcttgt gcgataattc
     3121 gccatggctg acgactccct cgcatctctc aaacttcccc cgcattccgt agaagccgaa
     3181 cagtcggtgc tgggtggtct gctgctggaa aacgacgcgc tcgacagaat cgccgatatc
     3241 ctcacggcgg acgattttta tcgtcacgac caccgtgtca tttacgaaca catcagccgc
     3301 ctgatcgagc acagcaagcc ggccgacatc gtgaccctgg ccgaggcgct ggaaagcacc
     3361 gcgcaactgg ccggggtggg cggtatcgcc tacctgggtg cactggcgca gaacaccccc
     3421 tccgccgcca atatccgccg ctacgccgaa atcgtgcgcg gccgcgctgt catgcgtcaa
     3481 ctggtgcaag tcggctccgg catcgccgag agcgcgctca gcccgcaggg caaggatgtg
     3541 gctcagttgc tggacgaggc ggaagccagg atcttcgaaa tcgccgaagg cggacaccga
     3601 agttcccaag gctttatcga tatcaagacc atcctgccgc aggtggccga ccgcatcgac
     3661 cagctgttcc agcgtgacaa tgccagcgac gtcaccggca tacctacagg atttactgat
     3721 ctcgactcca tgacttccgg cctgcagccg ggtgatcttg tcatcgtcgc cggacgcccc
     3781 tcgatgggca agaccgcctt ctcgctcaac atcgccgaaa acgtggcact ggataccggc
     3841 ctgccggtgg cggtattttc catggaaatg gccgcgacgc agctggcgat gcggatgata
     3901 ggctcggtcg gacggctgga tcagcacaag atgcgcaccg gacggctgga agacgaggac
     3961 tgggtgcgtc ttaccaccgc gctgggcaag ctcaacgacg cccccttgtt catcgacgaa
     4021 agcgctggcc tcaacgcact ggaagtgcgt gcccgtgccc ggcgtttgca ccgccaatgc
     4081 ggcaagctgg ggctgatcgt tctggactac ctgcaactga tgtcggccac ccgctccggt
     4141 gaaaatcggg ccactgaaat ctctgaaatc tcccgctcgc tcaaggcctt ggcgaaggaa
     4201 cttgatgtgc cggtggtggc gctttcccag ctcaatcgcg gccttgagca gcgccccaac
     4261 aagcgcccgg tgatgtccga cctgcgcgaa tccggcgcca ttgagcagga tgccgacgtc
     4321 atcctgttca tctaccgcga tgaagtctac aaccctgaca gccaggacaa gggcacggcc
     4381 gaaatcatca tcggcaagca gcgtaacggc cccatcggca ccgtgcgcct gaccttcctc
     4441 ggcgagcata cgcggtttga gaatttcgct agtaccggcg gctatcacgg cggcagcgag
     4501 tactgatacg gctcgacacc agctttccca tgagtcgtcc catttatgcc accatccatc
     4561 tcgatgccct gcgtcacaac ctgaccgtgg ccaaacgcca cgcgccggat tccagcatca
     4621 tggcggtcat caaggccaac ggctacggcc acggcttgct gcgcgtggca cacagcctgg
     4681 acgcggccga aggttttgcc gtgctggggg tggaggaggg cgtcgcgcta cgcgaggctg
     4741 gcttccggca gaccatcctg ttgctggaag gcttgttcga ggcggcggaa ctgcctgtcg
     4801 ctgcacagca tggcttcacg gtcgtggtgc attccgggcc gcagttgttg atgctggaag
     4861 ctgcgcggaa tgtgcccgcc gtggacgtgt tcctcaaggt caacaccggc atgaaccgcc
     4921 tgggcgttcc cgtcgggcgc ttctggagct tctacgaccg cctgcgcgcc tgcaagacgg
     4981 caggcaagct gaccttgatg acccactttg ccaccgccga cgagccggcc ggcgtggcag
     5041 agcagttctc gctgctcgac gacctcacgc gcgaaattgc catgccgcgc agcatggcca
     5101 attccgccgc caccctgcgc tacccgcaca cccacgccga ctgggtgcgg cccggcatca
     5161 tgctgtacgg cgccacgccg tttgctgatc aatccgctgc ggaacttgga ctgaagcctg
     5221 ccatgtcgtt gtccagccag gtcatcgcag tgcagaacct gcaagccggc cagagcgtgg
     5281 gctacggcca ccgcttcacc gccgaacgcc agacccgcgt cggggtagtc gcctgcggct
     5341 acgccgacgg ctacccgcgc cacgcgggcc aatatccggc tggtgaaaac cagcgtggtg
     5401 cgcccatcgt cgtggccggc cagttgacgc gcaccatcgg ccgcgtctcc atggacatgc
     5461 tgtacgccga cctcaccaat atcccccagg ccgacatcgg tagcccggtg gaactgtggg
     5521 gcgcgcaagt gccggtggat gacgtcgcaa ctgcgtccgg caccgtaggt tacgagctgc
     5581 tgtgtgctct ggcgccgcgc gtgccggtcg tgggggtctg atggccaagc aaaaaagtgt
     5641 ctatacctgc accgaatgcg gcgggcagac gcccaagtgg caaggtcagt gcccgagctg
     5701 taatgcctgg aatacgctgg tggagactat cgccgaggcg gcgcccgcca accgctatgc
     5761 cgcactggca ccagcggccg gcctgcagaa gctgtccgag gtggaggcgg ccgaggtgcc
     5821 gcgccagccg accggcatca tggaattcga ccgcgtgctg ggcggtgggc tggtgccggg
     5881 cggggtggta ctgatcggcg gtgaccccgg catcggcaaa tccacgctgt tgctgcaaac
     5941 gctttgccat atcggcgacc agcgcaaggt agtctacgtc agtggcgagg agtcggcgca
     6001 gcaaatcgcc atgcgggcta aacgcttatc tctggacgcc agcccgctgc aattgctggc
     6061 tgaaatcaat ctggaaaaaa ttctttccac gctgcaagcg caccaacctg atgtggcggt
     6121 gatcgattcc atccagaccg tgtattccga ggcgctgcag tcggcgcctg gctcggtggc
     6181 gcaggtgcgc gaatgctcgg cgcaactgac gcggctggcc aagcagcttg gtatcaacgt
     6241 gatcctggtc ggccatgtaa ccaaggaagg ttcgctggcc ggcccgcgcg tactggagca
     6301 catcgtggat accgtgctct acttcgaagg cgatcccaat tccagcttcc gcctgatccg
     6361 tgccttcaag aatcgcttcg gtgcggtcaa cgaactgggt gtgttcgcca tgaccgagaa
     6421 gggtctgcgc gaagtcagca acccttccgc attgttcctg tcgcatcacg ccgaacaggt
     6481 ggccggctcc tgcatcaccg tgacgcagga aggcacgcgg ccgttgctgg tggaagtgca
     6541 ggcgctggta gatgaagccc atgcgcctaa tccgaaaaga ttatgtgtag gtctggaaca
     6601 gaaccgcctc gccatgttgc tggcagtgct gcaccgccac gccggggtgg cctgctttga
     6661 tcaggacgtg ttcgtcaacg ccgtcggcgg cgtcaggatt gccgaaccgg cagctgatct
     6721 tgcagtgttg ctggcaattg tatcttcatt aaaaaacaaa caattgcata ataaacttat
     6781 tgtatttggt gaagtcgggc tggccgggga ggtgcgtcct gtgcaacgcg gccaggaacg
     6841 tctgaaggag gccgccaaac tgggctttac ccaagccatt gtgcccaagg ccaacctgcc
     6901 caagcagccg atcaagggca tcgaggtatt cggcgcggag cggctggaac aggcactggc
     6961 gcggttgcgt gaagcttaag acgtagtttt ctgcgcctcg aacacctgcc cgctgacccc
     7021 ggcgctgtcg tggcccatca gatacagata taacggcatg atggcctctg gttgaggcag
     7081 ttttccgtgc agttcgccag gatgggattt cttgcgctgc gggcattgca ccgggccggg
     7141 gatgagggcg ttgatgcgca gtttggggaa ggtttccagt tccagcgccc agatttttac
     7201 cagcgtctcc gccgcgccgt tggagatagc gtgtgcgccc cagaacgccg ccgggtggtg
     7261 cgcggcggtg gtggaagtca cgatcaccga ggcgtccggc gcgcgcttga gcagcggcag
     7321 gcaggccttg gtcagcagga aaggcgcgac ggtgttggtg cgtagcatgc cgagcatctg
     7381 ttcctgggtc tggatttcca gcgggctcag gttgtcgaag cggctggcgt tgtgcagaat
     7441 gccgtcgagt cggccgatct gctggtagat gccttccgcc agccccttga attcctcttc
     7501 gccggccttc tccaggtcga gcgggaagat cagcggttgt gggccgccgg cggcttcgat
     7561 ctcgtcgtac actgcttcca gcttcttcac cttgcgcccc agcaggatca acgtggcacc
     7621 ctgtgcggcg aaggcgaggg cggcggcctt gccgatgccc tgtccggcac cggtgacgag
     7681 aatgacgcgg tccttcaggt gttgatgggg gggctggtag tcgttcatgg cgtgaggtgc
     7741 ttggtgatgg ttttcgccat tttacctagc gactttcatt cggtggggat tactgtaaag
     7801 ctcgcagtta ccgtggcttc gtcttcctcg atcaggacgc gttcgaccag ggataagcgc
     7861 ggaccgtggc gggcccacgc gataatgacg gaaatcgcgt catccggccc gcagaccaag
     7921 gcttccacgg tgccgtcgcg ccggttcctg acccagccgg aaatccccag cttttcggct
     7981 tcgcgccaca tggattcccg gtagcccacg ccttgcacca gcccatggat gcgcaaacgt
     8041 ctggctggca tggcatcagg cgctgacgag gttgcctgcc gggatggcaa gcaggccgtt
     8101 gcccggcagc agccattcca ggaacaggcc gaagatggcg atgaaaatgc tggcgaagaa
     8161 tgccgtccag aagtccgaga ccttgaagcc gctgaccagc gccgccacca gttgaatcat
     8221 cagcgcgttg atgactagca ggaacaatcc caacgtcaga agcgtcagcg gcagggtgag
     8281 cagaatcagg atcggtttca gaatggcatt gacgaatccc agcatcagcg cagaaaccag
     8341 cagcgacgat ttgctcgaga attccaggcc gctgaacagg tggctggcta cccacaatga
     8401 gaggctcatg atgccccagt gcaggaggaa gtcgtaaagt tgtggattca tgggttacct
     8461 atattttcca gaacaaggtg ggcgcatttt acaccgctac gcatggcgcc ttcgatggtg
     8521 gcagggtagt cgcctgccgt gtagtcgcca gccaggtaga agttcgtcag cagcgtacgt
     8581 tgcgccgggc gcgacaggtt ggggcggcag gtgaaggtgg cgcgcttttc ggcaatgacc
     8641 ttgtgccact cgggcggtgg taaatcgggg aaggcctgtg ccagttcaac cgtcacctcg
     8701 gcggccagcg cctcctgggt cagcgactga tgacggccct cggcgctgat caccactgcc
     8761 agcaggccat gctgttcgtc cagcgcgccg cggtccagca cccactgtgc atagccgccg
     8821 gtaaggccga tcatggggaa gggcaggcgc gtagctgccg gatattgcag atatacggta
     8881 taaattggct ggtagtcgaa tccagcacaa atcccggcgg catcttgcaa ttgatgcagg
     8941 ccggcggcca cctcgccgaa gcggaacggc gacactgcgg cgatgacatg gctgaactgc
     9001 cgcctgtcgc cgtcgttgtc tacgacgaaa ccgtcctttt cctgcaggat agccgttgcc
     9061 ggcgatccaa gacgaacttc gccgccatgc tgccgaatgt actcggccag cggctccgcc
     9121 agtagcaggg agaggtcaca gcgcggcagc agcaggtcgc tgtcggattt cgatcgggaa
     9181 aaactgtcgc gcagcacgtt gaggaacacc tgtgcgctgg cccggtccaa tggcgtgttc
     9241 aatgcggcca ggcagatcgg ctcccacagc cattgcagca gcttttgcgg ctgccggtgg
     9301 cctgccagga aggcgcttag cggctcatct tgtgccagcc tgaatccgct tagcttgagc
     9361 gatgccatga aacgcagcgc ggccaggcgc tcacgcttgg acaacccatc cgcccgcagc
     9421 aggccggcca gaatatgcag cggggcaggc aggtgggtgg aggcacgcag cctgaactgc
     9481 cggtgctgga tcagttgcag cggcagcctg tgcaaggctg tggaaagatc taccccggcc
     9541 aaccgcaaca ggcgaagggt ttcgctatag gccccaagca gaatgtgctg accgttgtcg
     9601 aggcgcttgc ctttccagtt gagtccgcgc gcgcggccgc ccaactggcg gctggcctcg
     9661 taaagagtca cggaaatgcc ctggctggcg agttcggcag cagcggccag cccggcacaa
     9721 ccgcccccga tgacggcgac gcgactcaat ttctgaccca cgtcttccat gccagccata
     9781 gtttgcgcag cggggtcagg gagatgcgct ggttgagcac tttttcacag ccaccggcac
     9841 ggatttcgtc cagcagggtg cggtagatcg cggccatgac aagtccggtc cgctgcgcct
     9901 tgcggtcggc ggcgggaagc tcggcgaagg ccttctcgta atagccttgc gcgcgctcga
     9961 tctggaattc cagcaactgg cgcaggttaa cggtttcgcg gccttgcagg atgtcggctt
    10021 ccggcacgcc gaactgctcc agttcgtccg ccggcagata aatgcggccg cgacgggcat
    10081 cctcgccaac gtcgcggacg atattggtca gctggaaggc gaggccgagg tcgtgggcgt
    10141 atttcagcgt cttgcggtcg cggtaaccga agatggaggc cgacaacagg ccgaccacgc
    10201 tggcgacacg gtagcagtag agctgcaatt ccttgaagtc gcggtagcgg ttctggtcga
    10261 ggtccatctc catgccgtcg atgatctcgc ggaagtgctc ctcggccagg ccatactcac
    10321 cgatcgcgaa ctgcagcgcc tgggtgaccg ggtgcgtcgg cttgcctgca tacaggttgg
    10381 caatctcgcc gcgccaccag ttgagcttgg tgcgcgccag ttcgctgtcc gagcattcgt
    10441 cggcgacgtc atccacctcg cggcagaagg cgtacaacgc ggtgatggcc tggcgcttgg
    10501 gcttgggcag gaacaggaag ctgtagtaga agctggagcc gctggcagcg gctttctgct
    10561 ggcagtactg gtgtggatcg ctcattctct gaccttttat ttcgcccgca ccgcacgata
    10621 cagcatgtaa gcccagtcgg tggaacgcag caccggtcgc tggttgaaca tgtcgccatg
    10681 cgacttgtgc agcttgcgca gtatggtttc gccgccggcg atgatggtgc gcatctccag
    10741 cccgatgcgg cctttcagca ccaaacccag cggggcgccg gattgcagaa gcttgcgggc
    10801 gcgttcgatc tcgaactgca tgaagcccca ccacatgccg ccggcatcgc cacgggcgat
    10861 ctgctgttcg gtgatgcggt attttttcat ttcgtcgagc ggaaaataga tgcgatcctt
    10921 gcggtagtcg atggcaacat cctgcaggaa gttgatgatc tgcaaggccg agcagatggc
    10981 gtcggaatag ccgatattgc gcggcgtggc ctcgccgtac aggtgcagca gcagacgtcc
    11041 gatggggttg gcggaacggc ggcagtagtc cacgacttcg ccaaaattct cgtagcgcgt
    11101 tttcaccacg tcctggctga aggcatcgag caggtcgtag aacggctgca acggcagctg
    11161 atgggtcgcg atcatggcgg ccagggtgtg acagaattcg gtttccggcg ggttgcccgc
    11221 agcaagacga tccagttcct tgcggaagcc atccagtagt gccagacgtt gttcaggcgc
    11281 cagatcgcct tcgtcgg
//
LOCUS       DAOMFA010000041        11095 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_605_length_11095_cov_5.007699, whole genome shotgun sequence.
ACCESSION   DAOMFA010000041 DAOMFA010000000
VERSION     DAOMFA010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 11095)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11095)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11095
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_605_length_11095_cov_5.007699"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1010
                     /locus_tag="PKW44_03420"
     CDS             <1..1010
                     /locus_tag="PKW44_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HQR50669.1"
                     /translation="YTIHLLLTHIYQNNLFEGAGSYSRQDIAQYYIDRITADIRLARP
                     MKITVDAGNGVAGAYAGKLFRALGCEVQELFCEVDGHFPNHHPDPSVPENLQDLIQAL
                     RNGNAELGLAFDGDGDRLGVVTRDGAIIYPDRQLLLFAADVLARQPGATIIYDIKSTR
                     NLHPWISEHGGQPLMWKTGHSLMKAKMQETGAALGGEMSGHIFIKERWYGFDDGLYAG
                     ARLLEILSKSANPSAVLNALPDSVSTPEQHVQMREGEPHALIAQLQKEAKFEGAQNII
                     TIDGLRVEYADGFGLMRPSNTTPVIVLRFEADNEAALRRIQEQFRKVILAAAPGAKLP
                     F"
     gene            1037..1597
                     /locus_tag="PKW44_03425"
     CDS             1037..1597
                     /locus_tag="PKW44_03425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR50670.1"
                     /translation="MKPLEIIPVRIAMIFLIILLVHWVDHFVPLYFIIVAGGAGIMLL
                     SRVKALPGLDFLSRLETSDRLAIWIILVVLFLIGYLHNTNTWMPEGSFLKHAENTSFW
                     KTELILAVSLVAPAVHWLVGMLLPRITTMNGQRFATEFALYVNFSGIALVLWTGEATF
                     SDSMLGAAFVLVVLAELTLYASRSDQ"
     gene            complement(1665..3296)
                     /locus_tag="PKW44_03430"
     CDS             complement(1665..3296)
                     /locus_tag="PKW44_03430"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HQR50671.1"
                     /translation="MTVRTVATKPFNDQKPGTSGLRKRVPAFQQPHYLENFVQSIFDS
                     IAAPQGATLAVGGDGRYYNREAIQIILKMAAANGFGRVLVGQGGILSTPAASCVIRKY
                     ATFGGIILSASHNPGGPDGDFGIKYNIANGGPAPEKVTEAIFACSKAIAEYRILEAGD
                     VALDVIGETRLGDMRVQVIDPVRDYADLMESLFDFAAIRALLAGGFRLKFDAMHAVTG
                     PYAKEIIEKLLGAPAGSVMNAIPLPDFGNGHPDPNLTYAHELVEIVYGADAPDFGAAS
                     DGDGDRNMILGKRFFVTPSDSLAVLAANATLAPGYSKGLAGIARSMPTSAAADRVAQA
                     LDIPCFETPTGWKFFGNLMDAGKVTLCGEESFGTGSDHVREKDGLWAVLFWLNVVAAR
                     KQSVETIVREHWAKYGRNVYSRHDYEGLPTEAANGVMKHLHDSFARLVGAQLGSYKVL
                     FCDDFSYTDPVDGSVSTGQGVRIGFTDGSRIVFRLSGTGTEGATLRIYLEAYEADVSK
                     HHLDAQEALAELIAIALRLTELKARTGRDQPTVIT"
     gene            3455..4489
                     /gene="recA"
                     /locus_tag="PKW44_03435"
     CDS             3455..4489
                     /gene="recA"
                     /locus_tag="PKW44_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HQR50672.1"
                     /translation="MDENKSKALDAALSQIEKQFGKGSIMRMGDTDVSRDIQVVSTGS
                     LGLDLALGVGGLPRGRVVEIYGPESSGKTTLTLQVVAEMQKIGGVAAFIDAEHALDPQ
                     YAQKLGVNVSDLLISQPDTGEQALEICDMLVRSGSVDVVVVDSVAALTPKAEIEGEMG
                     DSHMGLQARLMSQALRKLTANIKRANTLVIFINQIRMKIGVMFGNPETTTGGNALKFY
                     SSVRLDIRRTGAIKKGDEVIGSETRVKVVKNKVAPPFKQAEFDILYGEGISREGEIIE
                     LGTNMKLVEKSGAWYSYKGEKIGQGKDNAREYLKEHPEIAQEIEAKIRANAGVASNLM
                     PTVIDEEADA"
     gene            4470..4925
                     /gene="recX"
                     /locus_tag="PKW44_03440"
     CDS             4470..4925
                     /gene="recX"
                     /locus_tag="PKW44_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HQR50673.1"
                     /translation="MRKPTPSLRERALGYLTRREYSRAELYRKLHPYAEEEDLDALLD
                     EFKQRGWLSDQRYADQMVHARKGKFGSLKVAHELRSQGVAEELVVKAAAEIGAEDKAT
                     ARNLWRKKFGATPQSREEWAKQARFLQSRGFGTETIKAVLRGDIEDLEQ"
     gene            4934..7552
                     /gene="alaS"
                     /locus_tag="PKW44_03445"
     CDS             4934..7552
                     /gene="alaS"
                     /locus_tag="PKW44_03445"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HQR50674.1"
                     /translation="MNSKQIRQAFLDFFASKGHTIVASSSLVPHGDPTLLFTNAGMNQ
                     FKDVFLGFDKRPYTRAATSQKCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFK
                     KDAIHYAWELLTEVFKIPADKLTVTVYAEDDEAYDIWANDIGVPKERIIRIGDNKGTR
                     YASDNFWMMGDTGPCGPCTEIFYDHGAHIPGGPPGSPDEDGDRFIEIWNNVFMQFNRD
                     EAGVMHKLPKPSVDTGMGLERIAAVLQGVHSNYEIDLFQTLIAAAARETRTTDLNFPS
                     LKVLADHIRACAFLIADGVIPGNEGRGYVLRRIIRRAIRHGYKLGARAAFFYKMVPDL
                     VAEMGEAYPELKAGERRVMDVLRQEEERFFETIEHGMAILETELAALSENRVFNGETA
                     FKLHDTYGFPLDLTQDICRERGVSVDTAGFGQAMVRQKEQARAAGKFKMAANLEYAGQ
                     ATTFHGYEKLENSARVLALYKDGSPVTVLNEGDLGVVVLDETPFYAESGGQVGDSGEL
                     RGENAIFAVEDTQKIQASVFGHHGVVKTGSLNVGDTVSARVNTTARMATMRNHSATHL
                     MHKALREVLGDHVQQKGSLVDSGKTRFDFVHNAPMTNEQIRQVERLVNAEILANTDCQ
                     ARVMDIESAQKTGAMMLFGEKYGDEVRVLDIGSSRELCGGTHVRRTGDIGLFSIMAEG
                     GVAAGVRRVEAVTGENALAYVQGMETALGGVAGSLKVLRADVPARVNALMDQTRRLER
                     ELAALKGRLASAKGDDLASQAVDAAGIKVLAATLEGADANALRETMDKLKDKLKSAVI
                     VLASTSDGKVSLAAGVTADLTTKLKAGELVNHVAQQVGGKGGGRPDMAMAGGTDAAGL
                     PQALSSVVDWVKLKSN"
     gene            7570..8796
                     /locus_tag="PKW44_03450"
     CDS             7570..8796
                     /locus_tag="PKW44_03450"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HQR50675.1"
                     /translation="MALIVQKYGGTSVGSPERIRNVARRVARYKALGHQVVVVVSAIS
                     GETNRLIGLAKEIMAEPDPRELDQMVSTGEQVTIALTAMALMDLGIKARSYTGAQVRI
                     LTDDAFTKARILSIDEANIRADLDAGYVVVVAGFQGVDEQGNTTTLGRGGSDTTGVAL
                     AAALKADECQIYTDVDGVYTTDPRIVPEARRLKTITFEEMLELASQGSKVLQIRSVEF
                     AGKYKVKLRVLSSFEEDGEGTLITFEEEDNMEQPIISGIAFNRDEAKITVLGVPDRPG
                     IAYQILGPIADANIDVDMIIQNVGADGTTDFTFTVHKNELNKALAILKDKVQTHIGAR
                     EIVGDDKIAKVAIVGVGMRSHVGIASEMFRTLAEEGINIQMISTSEIKISVVIDEKYL
                     ELAVRVLHKAYELENA"
     gene            8856..8949
                     /locus_tag="PKW44_03455"
     tRNA            8856..8949
                     /locus_tag="PKW44_03455"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8890..8892,aa:Ser,seq:gct)
     gene            8971..9047
                     /locus_tag="PKW44_03460"
     tRNA            8971..9047
                     /locus_tag="PKW44_03460"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9005..9007,aa:Arg,seq:acg)
     gene            9379..9879
                     /locus_tag="PKW44_03465"
     CDS             9379..9879
                     /locus_tag="PKW44_03465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR50676.1"
                     /translation="MKDLLDKLSSYNIFNYLLPGVLFAVFVDELTSFHISQKDIAVGV
                     FVYYFFGSVVSRIGSLLIEPLLRKLRIAKFAPYEDFVRVSKTDPKMEILSEVNNMYRT
                     ICSLMFCVGVVALYDRACASWPALRAVAPDVLIIGLFFLYLLSYRKQTAYITRRIEAV
                     AKQSKE"
     gene            10003..10848
                     /locus_tag="PKW44_03470"
     CDS             10003..10848
                     /locus_tag="PKW44_03470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR50677.1"
                     /translation="MPTEVHFMNVGQGNMTLLKLANGKVHLYDCNVTNDNEADILGYL
                     KKHIGAGTKIDVFICSHRDADHMRGIKKVHAKFPVQHVWDSGAVGTSPGCTEYLEYMQ
                     LRRTVGFTEVKHKTRYDYGNTRLRVMNAKNDDLADNANAQSIVIKVIQRNSSTDKEFA
                     SALLTGDTDAVTWQSIRSHYSDRDLSCAILLASHHGSITYFDDPSDDKNYYTGHIKAK
                     GPKMTIISVGDNAHGHPDKKALELYEKYSSGSNKGNKLYRTDEKGHIKLILKDDGGWT
                     LNIER"
     gene            complement(10884..>11095)
                     /locus_tag="PKW44_03475"
     CDS             complement(10884..>11095)
                     /locus_tag="PKW44_03475"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HQR50678.1"
                     /translation="MYAEKTIALALSGGGIRAMVFHLGVLKALTENGLLEKVSKISTV
                     SSTSINALTIDVARLSVPLVGDHHN"
BASE COUNT         2488 a         3276 c         3184 g         2147 t
ORIGIN      
        1 actataccat ccatctcctc ctcacccaca tctaccaaaa caacctgttc gaaggcgccg
       61 gcagctactc ccgacaggat atcgcgcagt actatatcga ccgcatcacc gccgacatca
      121 ggctggcccg ccccatgaaa atcaccgtgg acgccggcaa cggcgtggcc ggcgcctatg
      181 ccggcaagct gttccgcgcg ttgggctgcg aggtacagga actgttctgc gaggtagacg
      241 gccacttccc caaccaccac cccgaccctt cggtgccgga gaacctgcaa gacctgatcc
      301 aggcgctacg aaacggcaac gccgaactgg gactggcgtt cgacggcgac ggcgatcggc
      361 tgggcgtggt caccagggat ggcgctatca tctaccccga ccgccagctg ctgctgttcg
      421 ccgcagacgt gctggcgcgc cagcccggcg ccaccatcat ttatgacatc aaatccacgc
      481 gcaacctgca tccctggatc tccgagcacg gcggccagcc gctgatgtgg aaaaccggcc
      541 actcgctgat gaaggccaaa atgcaggaaa ccggtgccgc gctgggcggc gaaatgagcg
      601 gccacatctt tatcaaagaa cgttggtatg gcttcgacga cggcctctac gccggcgccc
      661 ggctgctgga aatcctcagc aagtcggcca atccgtctgc cgtcctgaac gctctgccgg
      721 acagcgtcag cacgccagag cagcacgtcc agatgcggga aggcgagccg cacgcgctca
      781 ttgcacaact gcagaaagaa gcaaaattcg aaggagcgca gaatattatc accatcgatg
      841 gcctgcgcgt ggaatacgcc gacggcttcg gcctgatgcg cccttccaac accacgccgg
      901 tcatcgtgct gcgcttcgag gcggacaacg aagccgcgct ccgacgcatc caggaacagt
      961 tccgcaaggt gatcctggcg gcagcgccgg gagcaaagct gccgttctaa gccaaaaaca
     1021 tgcaagagga attgtcatga agccactcga gatcatccct gtgcggatag ccatgatttt
     1081 tcttatcatt cttctggtgc actgggtgga ccactttgtc cccttgtact tcataatcgt
     1141 ggcagggggg gccggcatca tgctgctcag ccgcgtaaaa gcgcttccag gcctcgattt
     1201 tctgagcagg ctcgaaacct ctgaccgcct ggccatctgg atcatactgg tcgtgctgtt
     1261 cctgatcggc tatttgcaca ataccaatac ctggatgccg gaaggttcct ttctcaagca
     1321 cgccgagaac acttccttct ggaaaaccga attgatcctt gctgtgtccc tcgtagcgcc
     1381 ggctgttcat tggctggtgg gaatgctgct gccccggata accaccatga acggccagcg
     1441 atttgcgacc gagtttgcac tctacgtcaa tttttccggc atcgccttgg tgctatggac
     1501 cggcgaggcg acgttctcag attcaatgct gggagcggcg ttcgtgctgg tggtgctagc
     1561 tgagctgacg ctgtatgcca gtcgttccga tcagtaatcg aagtggccag cccctgaatt
     1621 gctccagagg cacgatcatc atcactctgc cgtcaagtta gaagtcaggt gatcaccgtc
     1681 ggctggtcgc gcccggtgcg cgctttcagt tcggtcagcc gcagggcgat agcgatcaac
     1741 tcggccagtg cctcctgggc atcgaggtga tgtttgctca catccgcctc ataagcttcc
     1801 aggtagatgc gcagggtcgc cccttcggta ccggtgccgg acaggcggaa cacgatgcgc
     1861 gagccgtcgg taaagccgat gcgcacgccc tgcccggtgc tgacgctgcc gtccaccggg
     1921 tcggtgtagc tgaagtcatc gcagaacagc accttgtagc tgcccagctg cgcgccgacc
     1981 aggcgggcga agctgtcgtg caaatgcttc atcacgccat tggcggcctc ggtcggcagg
     2041 ccttcgtagt cgtggcgcga atagacgttg cggccatact tggcccagtg ctccctgacg
     2101 atagtttcca ccgattgctt acgcgcggcg acgacattga gccagaacag caccgcccac
     2161 aggccgtctt tttcccgcac gtggtcggag ccggtgccaa agctctcctc gccgcacagc
     2221 gtcaccttgc cggcatccat caggttgccg aagaatttcc agccggtagg cgtctcgaag
     2281 cacggaatgt ccagcgcctg tgccacgcgg tcggcagcgg cactggtcgg catggagcgg
     2341 gcaatgccgg ccaagccttt gctgtagcct ggcgccagcg tggcgttggc cgccagcacc
     2401 gccagactgt cggaaggggt aacgaagaag cgcttgccca ggatcatgtt gcggtcgccg
     2461 tcgccgtccg acgcggcgcc gaaatcgggc gcgtcggcgc cgtagacgat ttccaccagt
     2521 tcatgcgcat aggtcaggtt ggggtcggga tggccgttgc cgaaatccgg cagcggtatg
     2581 gcattcatca cgctaccggc gggggcgccc aggagtttct cgatgatttc tttcgcatac
     2641 ggcccggtca ccgcgtgcat ggcgtcgaat ttaaggcgga agccgccggc cagcagcgca
     2701 cgaatcgcgg cgaaatcgaa cagcgactcc atcaggtcag catagtcgcg caccggatcg
     2761 atcacctgta ccctcatgtc gcccaggcgg gtctcgccga tcacgtccag tgccacgtcg
     2821 cccgcctcca ggatacggta ttcggcaatc gccttgctgc acgcgaagat cgcttcggtc
     2881 accttctccg gcgccgggcc gccattggcg atgttgtact tgatgccaaa atcgccatcc
     2941 ggcccgcccg ggttgtggct ggcggagagg atgatgccgc caaaggtggc gtatttgcgg
     3001 atcacgcagg aggccgccgg cgtggacagg atgccgccct gccccaccag cacgcggcca
     3061 aagccgttgg cggccgccat tttcaggatg atctggatcg cctcgcggtt gtaatagcgg
     3121 ccatccccgc ccaccgccaa ggtcgcgccc tgcggcgcgg cgatgctgtc gaaaatcgac
     3181 tgcacgaaat tttccaggta gtgcggctgc tggaacgccg gcacccgctt gcgcagcccg
     3241 gaagtgccgg gtttctggtc attaaaaggc ttggtggcta cggtgcggac ggtcatgtcg
     3301 ggttctccct ctcgagtgca ttgcgtgtct tccgtcattt tagccccagc gcccatgttt
     3361 cgggcaaaca agccataaag cccgccatcc gccttacctt agcatctgat ttatgacata
     3421 atttggccct tttacagggt tgggatcgca taccatggat gagaacaaaa gcaaggcact
     3481 ggacgccgca ctgtcgcaga tcgagaagca attcggcaaa ggctccatca tgcgcatggg
     3541 cgatactgat gtgtccaggg acattcaggt cgtttccacc ggctcgctcg gtctggatct
     3601 cgccctcggc gtaggcgggc ttccacgcgg ccgcgtggtg gaaatctacg gcccggaatc
     3661 ttccggcaag accacgctga cgctgcaagt ggtggccgag atgcagaaaa taggcggcgt
     3721 ggccgctttc atcgacgcgg aacacgcgct cgacccgcaa tatgcgcaga agcttggcgt
     3781 caacgtttcc gacctgctga tctcccagcc cgacaccggc gaacaggcgc tggaaatctg
     3841 cgacatgctg gtgcgctccg gctcggtgga cgtggtggtg gtggactcgg tcgccgcgct
     3901 cacccccaag gccgaaatcg aaggcgaaat gggcgactcg cacatgggcc tgcaagcccg
     3961 gctgatgtcg caggcgttgc gcaagcttac cgccaacatc aagcgcgcca atacgctagt
     4021 gattttcatc aaccagatcc gcatgaaaat cggcgtcatg ttcggcaacc cggaaaccac
     4081 caccggcggc aacgcgctca agttctattc ctcggtgcgc ctggatatcc gccgtaccgg
     4141 cgcgatcaag aagggcgacg aggtcatcgg ctccgagacc cgcgtcaagg tggtcaagaa
     4201 caaggtggcg ccgccgttca agcaggccga attcgatatc ctctacggcg agggcatttc
     4261 gcgcgaaggc gagatcatcg agctcggcac caacatgaaa ctggtggaaa aatccggcgc
     4321 ctggtacagc tacaagggcg agaaaatcgg ccagggcaag gacaacgcgc gcgaatattt
     4381 gaaggaacac ccggaaatcg cgcaggagat cgaggcgaag atccgcgcca acgccggcgt
     4441 cgccagcaac ctgatgccaa ccgtgatcga tgaggaagcc gacgcctagc ctcagggagc
     4501 gcgcgcttgg gtatctgaca cggcgcgagt attcccgcgc cgagctttat cgcaagctgc
     4561 atccctacgc cgaagaagaa gacctcgatg ccctgctgga tgaattcaag cagcgcggct
     4621 ggctctccga ccagcgctat gccgaccaga tggtgcatgc ccgcaaggga aaattcggta
     4681 gcctgaaagt cgcccatgaa ttgcggtcgc agggcgtggc agaggaactg gtcgtcaagg
     4741 ctgccgccga aatcggggcg gaggataaag caaccgcccg caacctgtgg cgcaagaaat
     4801 tcggcgccac cccgcaaagc cgcgaggaat gggccaagca ggccaggttc ctgcaaagcc
     4861 gcgggtttgg cactgaaacg attaaagccg tattgcgcgg cgacattgaa gatttggaac
     4921 agtagtgatg cacatgaaca gcaaacagat acgacaggct ttcctcgact ttttcgcctc
     4981 caagggccac accatcgtcg catccagttc attggtgccg cacggcgacc cgaccctgtt
     5041 gttcaccaac gccgggatga accagttcaa ggacgtgttc ctcggcttcg acaagcgccc
     5101 ttatacccgc gccgccacca gccagaaatg cgtgcgcgcc ggcggcaagc acaacgacct
     5161 ggagaatgtg ggttacacgg cccgccacca caccttcttc gaaatgctgg gcaatttcag
     5221 cttcggcgat tatttcaaga aagacgccat ccactacgct tgggaactgc tcaccgaggt
     5281 gtttaagatt cccgccgaca agctgaccgt cacggtctat gccgaggacg acgaagccta
     5341 cgacatctgg gccaatgaca tcggcgtgcc gaaagagcgc attatccgca tcggcgacaa
     5401 caagggcacg cgctacgcca gcgacaactt ctggatgatg ggcgacaccg gcccctgcgg
     5461 cccttgcacc gagattttct acgaccacgg tgcgcatatc ccgggcgggc cgcctggcag
     5521 cccggatgaa gacggcgacc gcttcatcga aatctggaac aacgtattca tgcagttcaa
     5581 ccgcgacgaa gcgggcgtga tgcacaaact gcccaagccc tcggtcgaca ccggtatggg
     5641 cctggagcgc atcgccgccg tgctgcaggg cgtgcacagc aactatgaga tcgacctgtt
     5701 ccagacactg atcgccgccg ccgcgcgcga aaccagaacg actgacctga acttcccgtc
     5761 gctgaaagta ctggccgacc acattcgcgc ctgcgccttc ctcatcgccg atggcgtcat
     5821 ccccggcaac gaaggccgtg gctacgtgct acgccgcatc atccgccgtg ccatccgcca
     5881 cggctacaag ctgggcgctc gcgccgcctt cttctacaaa atggtgcccg acctggtggc
     5941 tgaaatgggc gaggcctacc cggaactcaa ggccggcgag aggcgcgtca tggacgtgct
     6001 gcggcaagag gaagaacgct tcttcgagac gatcgaacac ggcatggcga tcctcgaaac
     6061 cgagctggca gccttatcgg aaaaccgggt ctttaatggt gaaaccgcat tcaagctaca
     6121 tgatacttat ggcttcccgt tagacttgac tcaggatatt tgtcgagaac gcggggtttc
     6181 tgtagatacc gcaggcttcg ggcaagcgat ggttcgccag aaggaacagg cacgcgccgc
     6241 cggcaagttc aagatggctg ccaatctgga atacgccggc caagccacca cattccacgg
     6301 ctacgaaaag ctggaaaatt ccgccagggt gctggcgctg tacaaggatg gcagcccagt
     6361 cacggtgctc aacgaaggcg acctcggcgt ggtggtgctg gacgaaacac ctttctacgc
     6421 cgaatccggc ggccaggtcg gcgacagcgg cgagctgcgc ggcgagaacg ccattttcgc
     6481 ggtggaagat acgcagaaaa tccaggccag cgtgttcggc caccacggcg tggtcaagac
     6541 cggctcgctc aacgtgggcg ataccgtcag cgcgcgcgtc aacaccaccg cccgcatggc
     6601 gaccatgcgc aaccactccg ccacccacct gatgcacaag gcgctgcgcg aggtgctggg
     6661 cgaccacgtg caacaaaagg gttcactggt ggatagcggc aagacccgct tcgatttcgt
     6721 gcataacgcg ccgatgacaa acgagcagat ccgccaggtg gagagactgg tcaatgccga
     6781 aatccttgcc aataccgact gccaggcacg cgtgatggat atcgaatccg cccagaaaac
     6841 cggcgccatg atgctgttcg gcgaaaaata cggcgatgag gtacgcgtat tggatatcgg
     6901 ctcttcgcgc gaactgtgcg gcggtacgca tgtgcgccgc accggcgaca tcggcctgtt
     6961 cagcatcatg gccgaaggcg gcgtggctgc cggcgtgcgt cgggtcgagg ccgtcaccgg
     7021 ggaaaatgcg ctggcctatg tgcagggcat ggaaaccgca ctgggcggcg tcgctggctc
     7081 cttgaaagtg ttgcgcgccg acgtgcccgc gcgggtaaat gcgttaatgg atcaaacacg
     7141 ccggctggag cgcgaactcg ccgcgctcaa gggcaggctg gcttcggcca agggcgacga
     7201 cctggccagc caagccgtag atgcggcggg catcaaggtg ctggccgcaa cgctggaagg
     7261 cgccgacgcc aatgccctgc gcgaaaccat ggacaagctg aaagacaaac tgaaatctgc
     7321 cgtcatcgtg ctggcttcga catccgatgg caaggtcagc ctggccgccg gcgtcactgc
     7381 cgacctcacg acgaaactca aagccggcga actggtcaat cacgtcgccc agcaagttgg
     7441 cggcaagggc ggcggacggc cggatatggc catggcgggc ggtacggatg ccgccgggtt
     7501 gccgcaggcg ctgtccagcg tagtcgactg ggtaaaacta aaatcaaatt gattcttaag
     7561 gtactttgaa tggcactgat cgtacagaaa tacggcggca cgtccgtcgg ctcccccgaa
     7621 cgcattcgca acgtagcccg ccgcgtggcg cgctacaagg cgcttggcca ccaggtcgtg
     7681 gtcgtggttt ccgccatctc cggcgaaact aaccggctga ttggcctggc caaggaaatc
     7741 atggcggaac ccgatccgcg cgaactggac cagatggtct ccaccggcga gcaggtcacc
     7801 atcgcgctca ccgccatggc gctgatggat ttgggcatca aggcgaggag ctataccggc
     7861 gcacaagtgc gcatcctgac cgacgacgcc ttcaccaagg cgcgcatcct gagtattgac
     7921 gaagccaata tccgcgccga tctggatgct ggttacgtcg tcgtcgttgc cggcttccag
     7981 ggcgtggacg aacaaggcaa caccaccacc cttggacggg gtggctccga cactaccggc
     8041 gtagcactgg ccgcagcgct caaggctgac gaatgccaga tctacaccga cgtggatggc
     8101 gtctacacca ccgacccacg catcgtgccg gaggcgcgcc gcctcaagac catcacgttc
     8161 gaggaaatgc tggaactggc gagccagggc tccaaggtgc tgcagattcg ctcggtcgaa
     8221 ttcgccggca aatacaaagt caagttgcgt gtgctttcca gcttcgaaga ggatggcgaa
     8281 ggcacactga ttacattcga ggaagaagac aacatggaac aaccgattat ttcaggcatc
     8341 gccttcaacc gcgacgaagc caagatcacc gtgctgggcg tccccgaccg ccccggcatc
     8401 gcctatcaga ttcttggccc catcgccgat gccaacatcg acgtggatat gatcattcag
     8461 aacgtcgggg cggatggcac caccgatttc accttcaccg tccacaagaa cgagctcaac
     8521 aaggcgctcg ccatcctcaa ggacaaggtg cagacccaca tcggcgcccg cgagatcgtt
     8581 ggcgacgaca agattgccaa agtcgccatc gtcggcgtcg gcatgcgttc gcacgtcggc
     8641 atcgccagcg agatgttccg caccctggcc gaggaaggta tcaacatcca gatgatctcc
     8701 acctccgaaa tcaagatctc cgtggtgatc gacgagaagt acctggaact ggctgtacgc
     8761 gtgctgcaca aggcgtacga gctggaaaac gcatagatcg agaatgcaag cacgtttgat
     8821 ttttttcggt taaacgagta ttcttgccac ccttcggaga gatggccgag tggtcgaagg
     8881 cacttccctg ctaaggaagc atacgggctt aaatctgtat cgagggttcg aatccctctc
     8941 tctccgccag tttggtataa tgccggacac gcgcccgtag ctcagctgga tagagtactt
     9001 ggctacgaac caaggggtca ggagttcgaa tctcttcggg cgcgccaaat atcaagggcc
     9061 acagcaatgt ggcccttttc atttgtacta ttcaatctca cttgtactat tcgcggcagc
     9121 aatctacccc gctgctgacg tcctagatca gttcacctca aggctgctac ggccaatttc
     9181 agatcttcgg ccaaagttgc cctccggcac atagtaggac aacgtcagaa atacataacc
     9241 aagcagacag acccagattg cacgaccaca actttgtgga atgatgagag gcgattatga
     9301 atgaacttgc ctccacctta tgcacagcat gataatcacc gcttcatatc ccaataggtc
     9361 gaacagccgg aggcatgcat gaaggatctg ctcgataaac tgagtagtta caacatcttc
     9421 aactacctgc tgccaggagt tctttttgct gttttcgtgg acgagctgac atcttttcac
     9481 atatcgcaaa aagatatcgc ggtcggcgtc ttcgtgtatt acttttttgg gtcggtggtt
     9541 agccgaatcg gttcattgct tatcgagcca ttactgcgga agttgagaat tgctaaattt
     9601 gccccctatg aggactttgt tcgcgtttcc aagacagacc ccaaaatgga aattctgtct
     9661 gaggtgaaca atatgtatcg tacgatatgt tctctgatgt tttgcgttgg tgtggtcgct
     9721 ttgtatgaca gagcatgcgc ctcttggcca gccctacgtg ccgtagctcc agatgttttg
     9781 atcattggcc tttttttcct gtatctactg tcctatagaa aacaaacggc ctatatcaca
     9841 aggcgaatcg aagccgttgc aaagcaaagc aaggaataga aataggtatc cattacggcg
     9901 aatctcactg tgtggaaata attgcatagt ggaccgagtt gtcgtaggtc cgttcggtgg
     9961 acaggcagat gggctaggaa caatccagaa ggagcagcca aagtgccgac agaagttcac
    10021 ttcatgaatg tagggcaggg aaacatgacc ttgctaaaat tggctaatgg caaggttcat
    10081 ctctatgact gcaatgtcac caacgataat gaggctgata tccttggcta cttgaagaag
    10141 cacattggtg ctggcacgaa aatcgatgtg tttatttgtt cgcatcggga cgctgaccac
    10201 atgcgcggga tcaagaaggt tcatgcaaaa tttcctgtgc aacatgtatg ggatagtggt
    10261 gcggttggaa cgtctcccgg ctgcacggag tatcttgagt acatgcaact gcgccgaacg
    10321 gtcggattca ctgaagttaa gcataagact cgctacgatt atggaaacac ccgtttgcgc
    10381 gttatgaacg caaagaacga cgacctggcc gataacgcca atgcccagag tatcgtcatc
    10441 aaagtcattc agcgtaactc ttcaactgat aaagagtttg ccagcgcgct cctcacaggc
    10501 gataccgatg cggtaacgtg gcaaagtatt cggagccact attccgaccg cgatctgtca
    10561 tgcgccattt tgttagccag tcatcatggc tctattacct actttgacga tccaagtgat
    10621 gacaagaact actacaccgg tcacatcaag gcgaagggtc caaaaatgac gatcatttca
    10681 gttggcgata atgctcacgg acatccggac aagaaagcgc ttgagcttta tgagaaatac
    10741 tcttccggct caaataaagg gaataagctc tatcggacag atgagaaggg acacatcaag
    10801 ctcattctca aggacgatgg cggttggacc ttgaatattg agaggtagcc gtaattcagc
    10861 aaagcatgcc gttccaagag atgttaattg tggtggtcac caacgagtgg aacgctcaag
    10921 cgagctacgt caatcgtgag cgcatttatt gaagtggagg agacagtaga aattttgctg
    10981 actttttcta ataacccatt ctccgtgagc gctttcaata cgcctagatg aaaaaccatg
    11041 gcgcggattc cgccacctga aagagctaat gcaattgttt tttctgcata catta
//
LOCUS       DAOMFA010000042        11077 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_608_length_11077_cov_5.231174, whole genome shotgun sequence.
ACCESSION   DAOMFA010000042 DAOMFA010000000
VERSION     DAOMFA010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 11077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11077
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_608_length_11077_cov_5.231174"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(40..939)
                     /gene="ttcA"
                     /locus_tag="PKW44_03480"
                     /pseudo
     CDS             complement(40..939)
                     /gene="ttcA"
                     /locus_tag="PKW44_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441073.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
     gene            complement(947..1699)
                     /locus_tag="PKW44_03485"
     CDS             complement(947..1699)
                     /locus_tag="PKW44_03485"
                     /EC_number="1.5.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pteridine reductase"
                     /protein_id="HQR50679.1"
                     /translation="MNDQLQDKVILITGGAKRVGASICRMLHAGGAKLMIHYRSSVGE
                     ARALQSELNLLRPQSAAIIQGDLHNLPMLAGLIHETIKHFGRLDVLINNASSYYATEI
                     GEIGEHEWDDLIGSNLKAPLFLSQAAAPELRKQQGCIVNITDMHVERPKKGYVVYSVA
                     KAGLATLTRSLAHELGPEVRVNAVAPGPVLWPESNPQFDELYRQRVISQTLLKRVGEP
                     EDIAKAVRFLIKDAPFITGQVIAVDGGRSLNL"
     gene            1752..2906
                     /locus_tag="PKW44_03490"
     CDS             1752..2906
                     /locus_tag="PKW44_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQR50680.1"
                     /translation="MNTSLPIPDPEQLEHGSRLIQIIRDEIAAQGGKVSFARYMELAL
                     YAPGLGYYSAGAKKFGTDGDYVTAPEISPLFAGTLARQAAQVLVATGGNVLELGAGTG
                     RLAAQVLLELQRLGALPQRYRILEVSAHLRSVQKETLAAGLPAELLAHVEWLEALPDT
                     FTGLVIGNEVLDALPVHVLTWRETGIFERGVAWKDGQFVWQECLVTDSRLLRTTQGLG
                     LPPGYISEVCPAASGLIASLAQMLEHGVLLFVDYGFPRCEYYHRQRASGTLMCHYRQL
                     AHDDPFFHPGLQDITAHVDFTAVAETGVSHDLQLLGYTTQAQFLINCGITELLGAVSP
                     ADMTAYLPLASQAQKLLSPAEMGELFKVVALGKNMQPLLGFGRGDKRHTL"
     gene            2936..3607
                     /gene="trmB"
                     /locus_tag="PKW44_03495"
     CDS             2936..3607
                     /gene="trmB"
                     /locus_tag="PKW44_03495"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HQR50681.1"
                     /translation="MERQRPIRSFVLRQGRLTAAQQRAVETLLPVFGISYAPTKLDLD
                     AVFGRAAPRILEIGFGMGSATAQIAQANPDQDYLGVEVHTPGVGNLLRLVQEHNLTNL
                     RVIQHDAVEVLHNMVADASLDAIHIFFPDPWPKKRHHKRRLLQAEFLHLLCDKLKPGG
                     YLHFATDWEEYAQWALEVLRAEPQLSNTVQDYAPRPEYRPLTKFEQRGLNLGHSVWDL
                     IFRRV"
     gene            complement(3560..4189)
                     /gene="yihA"
                     /locus_tag="PKW44_03500"
     CDS             complement(3560..4189)
                     /gene="yihA"
                     /locus_tag="PKW44_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HQR50682.1"
                     /translation="MPLFQNAQYFISAHHLRDLPPATGIEIAFAGRSNAGKSSAINTL
                     ANHTRLAYVSKQPGRTQLINFFSLGGDRYLVDLPGYGYAKVPEAMRKHWQLVLAAYLS
                     QRSSLHGLVLVMDSRHPLTPLDCQMLDWFGPTGKPIHVLLTKSDKMSRSEAMKTLAAV
                     RKELQQTWGNSSVQLFSSLKKQGVEEAEKVIGAWLNPPEDQVPDTVPEV"
     gene            4277..4882
                     /locus_tag="PKW44_03505"
     CDS             4277..4882
                     /locus_tag="PKW44_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019899817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HQR50683.1"
                     /translation="MNVRVMGVLVAGLLASMGAGAAGDAAKGAETATAVCAACHGPDG
                     NSVITTNPKLAGQFPEYLAKQLNEFKSGKRANAVMGPMSATLTTPEDVLNVAAYFAAQ
                     APKGGAAKSNGKGSDGERIYKGGILAKGVPACASCHSPNGAGIPVQFPRLAGQHAEYN
                     LNQLKAFRSGERANAPMMRVIASKMSDPEMEAVVDYIQGLK"
     gene            5001..>6354
                     /locus_tag="PKW44_03510"
     CDS             5001..>6354
                     /locus_tag="PKW44_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HQR50684.1"
                     /translation="MRFAVSLLTVLGIASIIGTVLKQNEPYANYVVQFGQFWFGLFES
                     LGLYDVYHAAWFLLILVFLVLSTSLCIYRNTPGMLRELRSFREHAMENSLRAFAHQAE
                     YQVDLPADETLNRLQRFLRDHDFSFKRTDQADGVLLAAKAGSYQRLGYILTHAAIVVI
                     CIGGLLDGNVPFKVQELLGLKKVETRDIPESQVPAPSRLSVANLSFRGNMTLPEGTSG
                     DVVFVRMRDGYLVQELPFTVALKDFRIEHYATGQPKSFESDVEIFDKQLKEPLKATIR
                     VNHPLIYKGIAIYQSDFQDGGSKLKFNVWPLVGVTEQPAVLEGQVFGRVKAGEGEQAL
                     TVEFDDFRFFNILTLEQGPKAKPHNVGPSVTFKVRDAQGQAREYLNYMQPMTLEGRAY
                     FVSGMRETQNQDFRYLRIPADADNSINDYMRLHAVLFDPALAPEIARRMAAKALPGEA
                     E"
     assembly_gap    6355..6454
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6455..7026
                     /locus_tag="PKW44_03515"
     CDS             <6455..7026
                     /locus_tag="PKW44_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HQR50685.1"
                     /translation="GEAEKGDLRQRFEESVAHLLGAFSQGGYTRIAEFIEKSVPQQDR
                     ERAAQTYIKIVGNAAFEALAMSRERAGKAVPQPDEATLQFVHDSLNSMSDLFFYGAPF
                     YLQLTDFEQRQASGLQLTRSPGKNLVYGGSLLLVLGIFAMIYLRERRIWLLVKPRTVL
                     FAMSAGRKNRDFEFEFARCRVQLQHLLEN"
     gene            7033..8178
                     /gene="ccsB"
                     /locus_tag="PKW44_03520"
     CDS             7033..8178
                     /gene="ccsB"
                     /locus_tag="PKW44_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HQR50686.1"
                     /translation="MESAQKYESPTPFLQRLTRADWIYTLALLAGALFAFNRYGVYMD
                     AYEQGFLFGAVLGFGWLGWFWKPVRLLLAGIALLSLFGISQYQDDLARGGQAFFLKFL
                     FSSQSAVMWMNVLFVLATATYWLALFARSDFTGRVATALTWSAVLMGFVGLMVRWYES
                     YLIAPDVGHIPISNLYEVFVLFCLITALIYLYYEGRYATRQLGGFVLLVVSAAVGFIL
                     WYTFDRQAAGIQPLVPALQSYWMKIHVPANFIGYGAFSLAAMVGSAWLLAQRGILASR
                     LPSLEVLDDLMYKSIAVGFAFFTIATILGAMWAAEAWGGYWSWDPKETWALIVWLNYA
                     AWLHLRLVKGLRGALSAWWALVGLLVTSFAFLGVNMFLTGLHSYGTL"
     gene            complement(8175..9854)
                     /locus_tag="PKW44_03525"
     CDS             complement(8175..9854)
                     /locus_tag="PKW44_03525"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQR50687.1"
                     /translation="MTHLARQQYALALLLLSLHGVMIWGFDTPLTQTLLLMHYGLFLL
                     WQPVWRGEQKLTIFSAVLFIIGAGLLVVMVNWWVLALWSAGLFGLLGGRVFGAQAGYS
                     RMTYMLAAGYLLGILLMWVVPNLLGTPPASGGVRFLVEYMLPLLPASLLFIPVNRAKE
                     STAPVLDLFYSLLLFMLTVILILGSFAIQVSGRTDYVRTLIHMLFGLAAALVVLSWLW
                     NPRAGFAGIGQLLSRYLLTIGMPFEDWLNKIAELANEQSSPSEFMVAAIGEVAALPWV
                     SGGAWKIDDGKGEFGHPATHHASFRFNDFQLTLHTRWPLTPALMLHIKLLTLILGEFL
                     EAKRREEALREQAYMRAVYETGSRMTHDIKNLVQSLSVLCAAAADGQKDSEALLMLMQ
                     RQLPQLNRRLALTLEKLQAPQAQGARMIRATTWWCELEQRHAGSNIVFTLHTVPSAAM
                     ANADMMDNVADNLLQNALEKARLEKTLHIAVTLRDENGLCLEISDDGKAIPEEVVQKL
                     FRTHTPSDNGLGIGLYHAARQAAQDGYELKLANNTDGNVRFTLCRQDDPTS"
     gene            10042..>10641
                     /locus_tag="PKW44_03530"
     CDS             10042..>10641
                     /locus_tag="PKW44_03530"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HQR50688.1"
                     /translation="MKKQSGFTLIELAIVLVIIGLLLGGVLKGQELIAQAKIKNVAND
                     INGTTVAIYGYQDRYKRLPGDDPGQSRWSVAGAPLAVAANQGDGDNIIEGGGYESTTN
                     TDENRKFWLNLRLAGFVAGSTATIAEGSTQPLNAAGGILGVQNSGLGITGTVVCVGSL
                     PAKIAQSIDSQMDDGNATTGSVRALIEGTPPTAVTTKAPA"
     assembly_gap    10642..10660
                     /estimated_length=19
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            10865..>11077
                     /locus_tag="PKW44_03535"
     CDS             10865..>11077
                     /locus_tag="PKW44_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019899808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQR50689.1"
                     /translation="MNDNIVEIDNLSFGYGGRTLHKGINMRFPRGKVVAIMGGSGSGK
                     TTILRLIGGQLRPSAGEVRVDGKVVHK"
BASE COUNT         2146 a         3090 c         3382 g         2340 t
ORIGIN      
        1 cgctccttga gggtcttgcc tgcaaacggg tttttcatct caagctaccc ttctgatgaa
       61 ttcaatacgg ttgccgtctg tgcctgcaaa gctcagcgcg gcatcttcat cgagatcgcc
      121 gaacagcggg tcggcttcgc tggtctggct ggagagcgac ttgaagtcga acagcccggt
      181 atccgccagg tgcgaaggcg cgacgttggt cagggcgcgg aatatcttct cggtgcgccc
      241 gggatactca tgttcccatt catgcagcat ggccttgatt ttctggcgtt gcaggttttc
      301 ctgcgagccg cacaggtcgc acgggatgat cgggaactcc atgccgcgcg cataggcggc
      361 gatatccttc tcggcgcaat aggccagcgg gcggatgacg atgaactcgc cctggtcggt
      421 cgccagcttg ggcggcatgg ccttgagctt agcgccgaaa aacatgttga gaaacagcgt
      481 ctgcacgatg tcgtcgcggt ggtggccgag tgcgatcttg ttcgcgccca gttcctgcgc
      541 ggtgcggtag atgatgccgc ggcgcaggcg cgaacacagc tagcaggtgg ttttgccttc
      601 cggcactttc tccttgacga tcgaataggt gtccgcttcc acgatgcggt actccacgcc
      661 cagtttttca aagtattccg gcaggatgtg ttcgggaaaa cccggctgtt tctgatccag
      721 gttcatggcg atcagcttga aatccaccgg cgcgcgttgc tgcaaggcca gcaacagcat
      781 cagcagcgcg tgcgaatcct tgccgccgct catgcacacc aggatggtgt cgccatgctc
      841 gatcatgttg tagtcgccga tggccttgcc caccgagctg atgagcttgt tgcgcagctt
      901 gaggaaattg ccggagacgt gttcagggta atgctgcatg ggaatcctac aggttaagcg
      961 aacggccgcc gtccaccgca atcacctggc cggtgatgaa aggggcgtct ttaatcagga
     1021 aacgcactgc cttggcgata tcctcgggtt cgccgacgcg cttcagcagc gtctgcgaaa
     1081 tgacacgctg acggtacagt tcgtcgaatt gcgggttgct ctctggccac agcaccgggc
     1141 cgggggccac ggcgttgacg cgcacttccg gccccagttc gtgcgccagc gagcgggtga
     1201 gggtcgccag cccggccttg gccacgctgt acaccacgta gcctttcttg gggcgctcga
     1261 cgtgcatgtc ggtgatgttg acgatgcagc cctgctgttt gcgcagttcc ggtgccgctg
     1321 cctgcgacag gaacagcggc gctttcaggt tggagccgat cagatcgtcc cattcgtgtt
     1381 cgccgatttc gccgatctcg gtggcgtaat agctggaggc attgttgatg agtacgtcca
     1441 gccggccgaa atgcttgatg gtctcatgga tcagcccggc cagcatgggc aggttgtgca
     1501 ggtcgccctg gatgatggcg gccgactgcg gacgcagcaa attgagctcg gattgcagcg
     1561 cgcgcgcctc gcccaccgag ctgcggtaat gaatcatcag cttggcgccg cctgcgtgta
     1621 gcatgcggca gatggaagcg ccgacgcgtt tggcgccgcc ggtgatgagg atgactttgt
     1681 cttgcagttg atcgttcatg ggaggtcctg aagtcagggg gtataattta ccataaccgt
     1741 cggatattcc cgtgaacaca tcattaccaa tccctgaccc ggaacagctg gaacatggca
     1801 gccgcctgat ccagatcatc cgcgacgaaa tcgccgcgca aggcggcaag gtcagctttg
     1861 cccgctacat ggaactggca ctgtatgcgc ctggcctcgg gtattacagc gccggggcga
     1921 aaaaatttgg cacggatggc gactacgtta ctgcgccgga aatctcccca ttatttgccg
     1981 gcacgctggc gcgacaggct gcacaagtgc tagttgctac cggcggcaat gtgctcgagc
     2041 tcggcgccgg caccggccgc ttggcagcgc aggtgctgct ggaattgcag cgactgggag
     2101 ctcttccgca gcgctaccgc attcttgaag tcagcgcaca cttgcgctca gtgcagaagg
     2161 aaacattggc agctgggctg cctgccgaat tgctggcgca tgttgaatgg ctggaagcgc
     2221 tgcccgacac gttcacaggg ctggtgattg gcaacgaggt gctggacgca ttgccagtgc
     2281 atgtgctgac gtggcgcgaa actggcatat tcgagcgtgg cgtagcgtgg aaagacggcc
     2341 agtttgtgtg gcaagaatgc cttgtgaccg acagccgtct gctgcgaacg acgcaagggt
     2401 tggggctgcc gcccggctat atcagcgagg tttgcccagc cgccagcggg ctgatcgcca
     2461 gcttggcgca aatgctggaa catggtgtgc tgctgtttgt ggattacggc tttccgcgtt
     2521 gcgagtacta ccatcggcaa cgagccagcg gcactttgat gtgccattac cgccagcttg
     2581 cgcacgacga cccgtttttc caccccggcc tgcaggacat cacggcgcac gttgatttca
     2641 ccgccgtggc cgagactggc gtgtcgcacg acttgcaatt gctgggctac accacacagg
     2701 cgcaattcct gatcaactgc ggcattactg agttgctggg cgcggtttcg cctgcggata
     2761 tgacggccta cctgccgttg gcgtcgcagg cgcagaagct gctgtcgcca gcagaaatgg
     2821 gggaactgtt caaggtagtg gcgctgggga aaaacatgca acctttgctg gggttcggcc
     2881 ggggcgacaa gcgccatacg ctataatgcg ccctttttgc gccggttgaa tcagcatgga
     2941 acgccaacgc cccatccgta gttttgtcct gcgtcaggga cgtctcaccg ccgctcagca
     3001 gcgtgcggtg gaaaccctgc tgccggtgtt cggcatttcc tacgcgccca cgaagcttga
     3061 cttggatgct gtgtttgggc gggcagctcc caggattctc gaaatcggct tcggtatggg
     3121 ttcggccacg gcgcaaatcg cgcaagccaa tccggaccag gactacttgg gagtggaagt
     3181 acatacgccg ggcgtaggca acctgctccg tttggtgcag gagcataacc tgaccaacct
     3241 gcgcgtgatc cagcacgatg cggtggaagt gctgcacaat atggttgctg atgcctcgct
     3301 tgacgccatt cacatcttct tcccggaccc ctggccaaag aagcgccacc acaagaggcg
     3361 gctgttacag gcggaattcc tgcatctgct gtgcgacaag ctcaagccgg gcggctatct
     3421 gcatttcgct accgattggg aagaatatgc ccagtgggcg ctggaggtgc tgcgggccga
     3481 gccgcaactg agcaataccg tgcaggacta tgcccctagg ccggaatatc ggccgctgac
     3541 caagttcgag cagcgcggcc taaacctcgg gcacagtgtc tgggacttga tcttccggcg
     3601 ggtttagcca ggcgccgatc accttctccg cctcctccac cccttgtttt ttcaggctgg
     3661 agaagagttg tacgctggag ttcccccaag tctgctgcaa ttccttacgc accgcggcca
     3721 gcgttttcat ggcttcgctg cgtgacatct tgtccgattt ggtgagcagg acgtggatgg
     3781 gtttgccagt ggggccgaac cagtccagca tctggcagtc gagcggggta agcgggtggc
     3841 gcgagtccat gaccagtacc aggccatgca ggctgctgcg ctgcgacagg taggcggcca
     3901 gcaccagctg ccagtgcttg cgcatggctt ccgggacttt ggcatagcca tagccgggca
     3961 aatccacaag gtagcggtcg ccgcccaggc tgaaaaaatt gatgagctgc gtgcgccccg
     4021 gctgcttgct gacgtaagcc aggcgcgtgt ggttggccag ggtgttgatg gcgctggatt
     4081 ttcctgcgtt ggagcggccg gcaaaggcga tttcgatacc agtggcgggt ggcagatcac
     4141 gcaggtgatg cgccgagata aagtattggg cgttctggaa taggggcatg gcggctgaat
     4201 gctatcacga acccccgaaa ttggataaaa tgccgcgatt caggtgtaag tattcaacca
     4261 ttttggggtg tgcgtgatga atgtgcgggt gatgggggta ttggtagcag gtctgctggc
     4321 ttcgatgggt gccggcgcag caggcgacgc tgccaagggc gcggaaacgg ctactgcggt
     4381 ttgcgcggca tgtcatggcc ccgatggcaa tagcgtgatt accaccaatc ccaagctggc
     4441 cgggcagttt ccggagtatc tcgccaagca actcaacgaa ttcaaatccg gcaaacgcgc
     4501 taatgcggtg atggggccga tgtcggccac tctgactacg ccggaagacg tgctgaatgt
     4561 ggcagcatac tttgctgcac aggcgcccaa ggggggtgcc gccaaaagca acggcaaggg
     4621 ctcggatggc gaacggattt acaagggcgg catcctcgcc aagggcgtgc cggcttgcgc
     4681 ttcctgtcac agtccgaacg gcgccggcat tccggtacag tttccgcggc ttgccggcca
     4741 gcacgccgag tataacctca accagttgaa ggcgttccgc tctggcgaac gtgccaatgc
     4801 gccaatgatg cgcgtgatcg cttccaagat gtctgacccg gagatggaag cggtggtcga
     4861 ttacattcaa gggttgaagt aatccgattc gatactgata cgttaaccgg gggtggcaat
     4921 tgccaccccc taattgttta tacatcttga ataaccctaa actatccagg tcccgcgcac
     4981 tatatgagtt gctgagctca atgcgcttcg ccgtcagtct gctgacggtg ctgggcatcg
     5041 cctccatcat cggcactgta ctcaagcaga acgagcctta cgccaactac gtggtgcagt
     5101 tcggccagtt ctggttcggc ctgttcgaat cgctggggct gtacgatgtc taccatgccg
     5161 cctggttcct gttgatcctg gtgtttctgg tgctttccac cagcctgtgc atctatcgca
     5221 atacaccggg catgttgcgc gagctgcgtt cgttccgcga gcacgccatg gaaaactcgc
     5281 tgcgcgcctt cgctcatcag gccgaatatc aagtcgacct gccggccgac gaaaccctga
     5341 atcggctgca acgtttcctg cgggatcacg acttcagctt caaacgtacc gaccaggcag
     5401 atggcgtgct gctggcggcc aaggccggca gctaccagcg tctgggctac atcctgaccc
     5461 atgcagctat tgtcgtgatc tgcatcggcg gcctgctgga cggcaacgtg ccgttcaagg
     5521 tacaggaatt gctgggcctg aaaaaagtgg aaacgcgcga catcccggaa tcgcaggtgc
     5581 cggcgccgag caggctatcg gtagccaacc tgtcgttccg cggcaacatg acgctgccgg
     5641 aaggcaccag cggcgatgtg gtattcgtgc gcatgcgcga cggttatctg gtgcaggagc
     5701 tgccgtttac cgtggcgctc aaggatttcc gcatcgagca ttacgctacc ggccagccca
     5761 agtcgttcga gagcgatgtg gagatttttg acaagcagct caaggaaccg ctcaaggcca
     5821 ccattcgcgt caatcatcct ttgatctaca agggtatcgc catttaccag tcggatttcc
     5881 aggatggcgg atccaaactc aagttcaacg tctggccgtt ggtcggcgtt acggagcagc
     5941 ctgccgtgct cgaggggcag gtgttcggca gggtcaaggc cggcgaaggc gagcaggcgc
     6001 taacggtcga gttcgacgat tttcgtttct tcaatatcct gaccctggaa cagggcccca
     6061 aggccaagcc gcataacgta ggtcccagcg tgaccttcaa ggtgcgggat gcgcaagggc
     6121 aggcgcgcga atacctcaac tacatgcagc cgatgacgct ggaaggccgt gcctacttcg
     6181 tttccggcat gcgcgaaaca cagaaccagg acttccgcta tctgcgcatt ccggccgatg
     6241 cggacaattc catcaacgac tacatgcgct tgcatgccgt gctgttcgat cccgcgctgg
     6301 cgccggaaat tgcacggcgc atggcggcca aggccttgcc gggagaggcc gaaannnnnn
     6361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncgggag aggccgaaaa aggtgacttg
     6481 cgtcagcgtt tcgaggagag cgtggcgcat ttgctgggcg cattttcgca gggcggttat
     6541 acccgtatcg ccgaattcat cgagaaatca gtgccacagc aggatcgcga aagagcggcg
     6601 cagacctata tcaagattgt cggcaatgcc gcgttcgagg cgctggcaat gagccgcgaa
     6661 cgtgccggca aggccgtgcc gcaaccagac gaggccactc ttcagttcgt gcacgacagt
     6721 ctcaactcga tgagcgacct gtttttctac ggcgcgccgt tttacctgca gctcactgat
     6781 ttcgagcagc gccaggccag cggcctgcag ttgacccgtt cgcccggcaa gaatctggtg
     6841 tatggtggtt cgctgctgct ggtgctgggg atatttgcca tgatttacct gcgtgaacgc
     6901 cggatatggc tgctggtcaa accgcggacg gtgttatttg ccatgtctgc cggtcgcaag
     6961 aaccgcgatt tcgagttcga gtttgcgcgc tgccgcgtgc agttgcagca cttactggag
     7021 aattgagcca tcatggaatc tgcacagaaa tacgaatcgc caacgccgtt tctgcaacga
     7081 ctgacccggg cagactggat ttacacactg gccttgttgg ccggcgcgct gttcgccttc
     7141 aaccgttacg gcgtatatat ggacgcctac gagcagggct tcttgttcgg tgctgtgctg
     7201 ggcttcggct ggctgggctg gttctggaaa ccggtgcgcc tgttgctagc ggggatcgcg
     7261 ctactcagcc tgttcggcat cagccagtat caggatgacc tggcgcgcgg tggccaggcc
     7321 tttttcctca agttcctgtt ctccagccag tccgccgtca tgtggatgaa cgtgctgttt
     7381 gtactagcca ccgccactta ctggctggcg ctgttcgcgc gttcggattt caccggccgg
     7441 gtggctaccg cgctgacctg gagcgctgtg ctgatgggtt ttgtcgggct catggtgcgc
     7501 tggtacgagt cttacctgat cgcgccagac gtcgggcata ttcccatcag caacctgtat
     7561 gaagtgtttg tgctgttttg cctcatcact gcgctgatct atctctatta cgaggggcgt
     7621 tacgccacgc gccaactagg cggcttcgta ctgctggtgg tgagtgctgc ggtgggcttc
     7681 atcctgtggt acaccttcga ccgtcaggcc gccggcatcc aaccactggt gccggcgctg
     7741 caaagctact ggatgaaaat ccacgtgccc gccaacttta tcggctatgg ggcgttctca
     7801 ctggcggcca tggtgggcag cgcctggctg ctggcgcagc gtggcattct ggccagccgc
     7861 ctgccttcgc tggaggtgct cgacgacctg atgtacaagt ccattgcggt gggtttcgct
     7921 ttcttcacca ttgctaccat tctcggcgcc atgtgggcag cggaagcttg gggcggctac
     7981 tggtcctggg atcccaagga gacctgggcg ctgatcgtgt ggctcaacta cgccgcctgg
     8041 ctgcatttaa ggctggtgaa gggacttcgc ggagcgttgt cggcctggtg ggcgctagtc
     8101 ggcctgctgg tgaccagctt cgccttcctc ggcgtcaaca tgttcctgac tgggctgcac
     8161 tcctacggca cgctttagct tgtcggatcg tcctgccgac aaagggtgaa gcgcacattt
     8221 ccgtctgtgt tgttcgcgag tttcagttca tagccatcct gggctgcctg ccttgccgca
     8281 tggtagaggc cgatgccaag tccgttgtcg gagggagtgt gggtccggaa taacttctgg
     8341 accacctctt ccggaatggc tttaccgtca tcgctgatct ccaggcacag gccgttttca
     8401 tctctcagcg tgacggcgat gtgcagggtt ttctccagac gcgccttttc cagagcattt
     8461 tgcaagaggt tgtcggcgac attgtccatc atgtcggcat tggccattgc tgccgatggt
     8521 acggtgtgca acgtgaaaac gatgttgctg ccggcatggc gctgctcgag ttcgcaccac
     8581 catgtggttg cgcggatcat gcgggcgccc tgcgcttgcg gcgcctgcaa tttctccagg
     8641 gtaagcgcca ggcgccggtt gagctgaggc agttggcgct gcatcagcat caacaacgct
     8701 tcgctgtctt tctgcccgtc ggctgccgct gcacacagga cgctgagcga ctgcaccagg
     8761 ttcttgatgt cgtgcgtcat gcgcgacccg gtttcgtaaa cggctcgcat gtaggcctgt
     8821 tcgcgcagcg cctcttcccg ccgcttggct tccagaaact cacccaggat tagcgtcagc
     8881 agcttgatgt gcagcatcag cgccggcgta agcggccagc gggtgtgcag tgtcaactgg
     8941 aaatcattga acctgaagct ggcatggtgg gtggcagggt ggccgaactc gccttttcca
     9001 tcatcgattt tccaggcgcc accgctgacc catggcagtg cggcaacttc gccgatggcg
     9061 gcgaccatga attccgaggg gctcgattgt tcgttggcca gttcggcgat tttgttcagc
     9121 cagtcttcga atggcatgcc gatggtcagc aaataacgtg acagcagctg gccaatgccg
     9181 gcgaaacctg ctcgcggatt ccatagccaa ctcagcacta ccaaggccgc cgcaaggccg
     9241 aacagcatgt ggatcagggt gcgaacataa tcggtgcggc ctgagacctg gatggcgaag
     9301 ctgcccagta tcaggatgac agtcaacatg aacagcagga ggctgtagaa cagatccaga
     9361 acgggcgctg tgctttcctt ggcacgattg accgggatga acagcaggct ggccggcaac
     9421 agcggcagca tgtattcgac cagaaagcgc acgccaccgg atgccggtgg cgtgccgagc
     9481 aggttgggca ccacccacat cagcaaaatg cccagcaggt agcctgcggc gagcatgtac
     9541 gtcatgcgcg agtagccggc ctgtgcgccg aatacccgtc cgcccagcag gccgaataag
     9601 cccgcgctcc acaacgccag cacccaccag ttgaccatga ccaccagcag ccccgcgccg
     9661 ataatgaaca gcaccgccga gaaaatggta agtttctgct cgccgcgcca cactggctgc
     9721 cataacagga acaggccgta atgcatcagc agcagggttt gcgtgagcgg ggtgtcaaac
     9781 ccccagatca tcacgccatg caaactaaga agcagcaggg cgagggcata ctgctggcgc
     9841 gccaagtggg tcatgcggtt tcagcgctta cgggcgacaa aaatgtcagc aacttgtcat
     9901 gcatgatgaa atattgacat ctattctggg cgaaggaaag cgaatgccat gtatcggctg
     9961 gaaaaacatg gcgttacgcc aaaattttat ggtacgctta ttgctagttt acttgcaggc
    10021 taacaaccaa ggaactacaa aatgaaaaag caatccggat ttaccttgat cgaacttgcg
    10081 atcgtgctcg tgatcatcgg cttgttgctg gggggcgtcc ttaaggggca ggagctgatt
    10141 gcacaagcca aaatcaagaa tgtcgcgaac gatattaatg ggactactgt agcaatatat
    10201 ggttaccagg atcgttacaa gcgcctgcct ggcgatgatc caggccagag ccgatggagc
    10261 gtggccgggg cgccgctggc tgtggctgct aaccaagggg atggcgataa catcattgag
    10321 ggtggtggtt acgagtctac gactaatacg gatgaaaacc ggaaattctg gctgaacctt
    10381 aggctggcag gcttcgtggc cggcagtact gccacgatcg cggaaggatc cacccagccc
    10441 ttgaacgctg ccggcggaat tctcggtgtt cagaatagcg gactgggaat tacaggaacg
    10501 gttgtgtgtg tcggcagttt gccggcaaaa attgcccaat ccattgacag ccagatggat
    10561 gacggcaacg cgacgacggg aagtgtgcgt gccttgattg aggggactcc gccaacagcg
    10621 gtaacgacaa aggcgcctgc annnnnnnnn nnnnnnnnnn gtcgacaatg gcactaacct
    10681 ctacgtattg tgtaagagca tttgaccgtt gagccaaaga cgacaaagcc cgcttcggcg
    10741 ggctttgtcg ttgtggacac gccccccggt cagttgcctg aatttttgcc tgctcgtcga
    10801 agaactcttg tttcagatga tttggctgcc aaggcggaat aatttcaagt aaaatgccgc
    10861 gatcatgaac gataacatcg tagaaatcga taacctttcc ttcggctatg gcggccgcac
    10921 gctgcacaag ggcatcaaca tgcgctttcc gcgcggcaag gtggtggcca tcatgggcgg
    10981 ctccggttcc ggcaagacta ccatcctgag gctgatcggt gggcagttga ggccgagcgc
    11041 gggcgaggtg cgagtggacg gcaaggtagt gcacaaa
//
LOCUS       DAOMFA010000043        11071 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_610_length_11071_cov_5.152869, whole genome shotgun sequence.
ACCESSION   DAOMFA010000043 DAOMFA010000000
VERSION     DAOMFA010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 11071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11071
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_610_length_11071_cov_5.152869"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..328)
                     /locus_tag="PKW44_03540"
     CDS             complement(<1..328)
                     /locus_tag="PKW44_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HQR50690.1"
                     /translation="MQVISHPGFADQTINKSRFIAVADYCADERAVMELLRGLAHEHA
                     HANHLVFAYKVQNPGGGYLVRFHDAGEPCGTAGKPILQYIEGRDLVNVWVAMVRYFGG
                     VKLGTGG"
     gene            411..1715
                     /locus_tag="PKW44_03545"
     CDS             411..1715
                     /locus_tag="PKW44_03545"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HQR50691.1"
                     /translation="MQNADLLNRSLAAVWHPCTQMKAHETMPLVPIARGEGVWLYDFD
                     GRRYLDAVSSWWVNLFGHANPCISTALKDQLDKLEHVILAGFTHQPVVELSERLSKLT
                     RLGHAFYGSDGASATEIALKMSFHFWRNSGQPQKTNFISLQNSYHGETIGALSVTDVA
                     LFKDTYAPLLRQSTQVPSPDWRLAKAGESAEAYAMRCAAVLEAHLEQHWASTAALIVE
                     PLVQGAAGMAMYHPTYLQRARELCNRYQVHLIADEIAVGFGRTGTMFACEQAGIKPDF
                     ICLSKGITGGYLPLSVVLTTDSVYAAFYDDRTTRGFLHSHSYTGNALACRAALATLDI
                     FEQDDVIVANRTKATHFNALLAPLAAHPKVRNFRHLGMIWAFEVDSTDARFSHGFYQA
                     ALARGLLLRPINNTVYLMPPYVISEDEMAMLTEQLLQLVEQA"
     gene            1712..3127
                     /gene="dacB"
                     /locus_tag="PKW44_03550"
     CDS             1712..3127
                     /gene="dacB"
                     /locus_tag="PKW44_03550"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HQR50692.1"
                     /translation="MKLLALLLAGWLAITPALANGLPAPVAKALKAAGVPQDGVAFYV
                     QQVDQHQPRLSHRADQPMNPASTMKLLTTDAGLELLGPSYRWRTEIYGSVPPQDEVLN
                     GDLIIKGYGDPALTLENFWLLLRMLRQSGVRDIRGDLLLDRGYFDTSGNEPPGKFDGE
                     TYRAYNAAPDAMLVNFKATRFTLRGDPALGKVLITADPALPQLNIVNQVALSQVPCAD
                     WKDRIGYRVLREKDVVTVTFSGNYSVACGEKALELSILDDDVYVFELFRRMWQEQGGQ
                     ISGGLKNGSLPPGAQRLVQADSPPLADVIRLVNKYSNNVMARQILMTLGAERYGAPGS
                     PQSGALAVREWLAGKGMEWPELAIENGAGLSRNERISARHLGELLLSAYRSPVMPELM
                     SSLPIVAIDGTMQHRLKSSAVAGQAHLKTGSLDGVRAMAGYLLDARGRRWVVVFMANH
                     PAAAGSKAAQDALLEYVYALP"
     gene            3203..3670
                     /locus_tag="PKW44_03555"
     CDS             3203..3670
                     /locus_tag="PKW44_03555"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HQR50693.1"
                     /translation="MKHTSLFVGTLLLALATAGCNAADPQSQTKENPAMTLPVTVKEL
                     IQIDQKVGGVAEALPLNTVKVHYTGWLYDPSKPDGHGKKFDSSLDHGEAFEFMLGGGQ
                     VIRGWDEGVAGMKVGGKRTLIIPQHMGYGARGAGGAIPPYATLIFDVELLDVR"
     gene            complement(3756..5219)
                     /gene="ubiD"
                     /locus_tag="PKW44_03560"
     CDS             complement(3756..5219)
                     /gene="ubiD"
                     /locus_tag="PKW44_03560"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011310617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HQR50694.1"
                     /translation="MQYKDLRDFISQLEAQGELRRVRVPVSPQLEMTEVCDRVLKAGG
                     PAILFENVIGHSMPVLGNLFGTPHRVALGMGEESVEALREVGKLLAFLKEPEPPKGLR
                     DAWDKWPKFKKVLDMAPKIVSRAPCQEVVWEGDEVDLSRLPIQTCWPGDAAPLITWGL
                     TVTRGPLKERQNLGIYRQQVIAKNKVIMRWLPHRGGALDFRDWCAAHPGEPFPVAVAL
                     GADPATILGAVTPVPDTLSEYQFAGLLRGGKSEIVQCVSSDLQVPAGAEIVLEGHIAP
                     GETAPEGPFGDHTGYYNEVGKFPVFTITHIALRRDPIYHSTYTGKPPDEPAVLGVALN
                     EVFVPLLQKQFPEITDFYLPPEGCSYRLAVVSMKKQYSGHARRVMMGVWSFLRQFMYT
                     KFIIVTDDDVNIRDWKEVVWAITTRVDAARDTVIVENTPIDYLDFASPVSGLGSKMGI
                     DATNKWKGETSREWGRPIAMEEAVKSRIDALWPQLGL"
     gene            5323..5739
                     /locus_tag="PKW44_03565"
     CDS             5323..5739
                     /locus_tag="PKW44_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011310963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HQR50695.1"
                     /translation="MQARVKWVENVCFVGESETGHALVMDGAPESGGRNLGMRPMELL
                     LIGMGGCTSFDVVTILKKGRQQITDCYVELKAERAETVPKVFTKIHAHFVVTGRGLNP
                     AQVERAINLSAEKYCSASLMLGKTAEITHDFEIVEA"
     gene            complement(6318..6629)
                     /locus_tag="PKW44_03570"
     CDS             complement(6318..6629)
                     /locus_tag="PKW44_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020163745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2322 family protein"
                     /protein_id="HQR50696.1"
                     /translation="MPSFKENLETFPSIDGIAKLELLDAAGNLVGTIENKPGSQGSLR
                     LYLHLAQQFGSITPEAARVGCELYAEHTEDARLNPGKHPNIDRLFVLVEKGEALGVRL
                     A"
     gene            complement(6759..7379)
                     /gene="coq7"
                     /locus_tag="PKW44_03575"
     CDS             complement(6759..7379)
                     /gene="coq7"
                     /locus_tag="PKW44_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinone
                     monooxygenase"
                     /protein_id="HQR50697.1"
                     /translation="MLDRLIIEFDKGLRTVLAQAQSVRPFPDRDQVESPLSEQEKRHA
                     AALMRVNHSGEICAQALYNGQALTARNPATEAALREASQEETEHLAWCEKRIYELGSH
                     KSLLNPLWYAGSFALGALAGALGDKWNLGFLAETENQVDKHIEGHLQSLPPQDEKSRA
                     ILEQMKIDEAKHATTALHHGGAELPLPVKMAMKLTSKILTKTAYWI"
     gene            complement(7372..8574)
                     /locus_tag="PKW44_03580"
     CDS             complement(7372..8574)
                     /locus_tag="PKW44_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HQR50698.1"
                     /translation="MNAESISHLSKLAKEADRCVACGLCLPHCPTYRKTQSEADSPRG
                     RIQLISAVAQGRLPASKRFVQHIDLCLSCRACENVCPNGVAYGTLVDAARAIIALPAT
                     LIQRAVTWLLERRAMLSLAGHALRFAQISGLRLLAAAMLPKLRQADALLPAIPAQENW
                     RSVYPAEQVRGEVALFLGCISAIADADTLRATIFVLNRLGYTVHVPAEQCCCGGIARQ
                     SGDAAASARMLEKNARAFAALADMPLISVVSGCGAGLRDHFGKRATDIAAFLGQANWN
                     GIEPAQLEATIHVHDACSLRNTMRQHEEVYSLLRRIPGAEVKPLPGNTQCCGGAGAYM
                     LTQPAMARQLRDDKMSACRASSATLLATSNIGCALHIAAGLRDDGIKVEVAHPVVILA
                     RQLGWKNA"
     gene            complement(8610..10019)
                     /locus_tag="PKW44_03585"
     CDS             complement(8610..10019)
                     /locus_tag="PKW44_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HQR50699.1"
                     /translation="MRAKFEKFGLIGVGAILGIMLSLNYSAIADKDAKTQLPLEDLRA
                     FAEVFGKVKSDYVEPVEDKKLISEAISGMLSGLDPHSAYLDEDAFKDLQAGTQGEFGG
                     LGIEVGMEDGFVKVVSPIEDTPAYKAGLKPGDLIIKLDETPVKGMTLTEAVKLMRGKP
                     DTKIVLTILRKGENKPIVVTLTRAIVKVQSVKSKPSVEPGYGYVRITQFQEHTGADLA
                     KALKAMVDENKDGLKGVVLDLRNNPGGLLNAAVGVSAAFIPKGELVVYTEGRVPDAKM
                     RLTASPENYVRSGPAEDYIKGLPPELKTVPMVVLINGGSASASEIVAGALQDHKRAII
                     LGTQSFGKGSVQTILPMNNGTAIKLTTARYFTPNGRSIQAKGIVPDIQAEEATVNNAE
                     QGMNFREADLTRHLSNPRDGEKPSAPEQPAKVIAPPAGDENDKSGDKNAPMEPGTKSD
                     YQFTQAMNLLKGLQILQKK"
     gene            complement(10170..10910)
                     /locus_tag="PKW44_03590"
     CDS             complement(10170..10910)
                     /locus_tag="PKW44_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR50700.1"
                     /translation="MKRTRLALILGALYSGIVAAGPADYVYMPTVEYGEREIEFKYGT
                     AEQRPDDERKQVSMLGFGYGATEYWFSEIYLKREREGSERLTLGEWENKFQLIETGKY
                     PFELGLITEIEFPFSDRRDTPYEFKLGPLLQTDVGRVQLNGNLLFERKFGPNDGESYN
                     TEMGYQWQVKYRWQPAFEFGAQGFGDMGEWNHWENSNAQSHRLGPAAFGKINLGDKQA
                     LKWNAAWLFGTTSASLDNNFRMQAEYEF"
BASE COUNT         2059 a         3363 c         3381 g         2268 t
ORIGIN      
        1 gcccgcccgt acccagctta actccgccaa aataccgcac catagccacc caaacattca
       61 ccaggtcgcg cccctcgata tattgcagaa taggcttgcc cgccgtgccg cagggctccc
      121 ccgcgtcgtg gaagcgcacg aggtagccgc ccccgggatt ctgcaccttg taggcaaaca
      181 ccaggtggtt ggcgtgcgca tgctcgtgcg ccagcccccg cagcagctcc atcaccgcgc
      241 gctcgtcggc gcaatagtcc gccaccgcga taaagcgcga cttgttgatg gtctggtcgg
      301 caaagccggg gtgggagatg acctgcattt tcaggttttt tgtgcggtga ctgttgttgg
      361 tggcatgcgc tgtgttgtaa taagcattct acttgatact gctttcagcc atgcagaacg
      421 ccgacctgct caaccgttcc cttgccgccg tgtggcaccc ctgcacacag atgaaagcgc
      481 acgaaacgat gccgctggta cccatcgccc gcggcgaggg tgtatggctg tacgacttcg
      541 atggcaggcg ttatctggac gcggtgagtt cctggtgggt taaccttttc gggcatgcca
      601 acccatgcat cagcaccgca ctcaaggacc agttggacaa gttggagcat gtgatactgg
      661 cgggctttac ccaccagccg gtggtggagc tttccgagcg gctgtcaaaa ctgactaggc
      721 tggggcatgc cttctacggc tcggacggcg cctcggcgac ggagatcgcg ctgaagatga
      781 gcttccattt ctggcgcaac agcggccagc cgcaaaaaac caatttcatc agcctgcaga
      841 acagttacca cggcgagacc atcggcgcgt tgtcggtgac cgacgtagca ctgttcaagg
      901 acacctacgc gccgttactg cggcaaagta cgcaggtgcc cagcccggac tggcggctgg
      961 cgaaagcggg cgaatcagcc gaggcctatg ccatgcgttg cgctgcggtg ctggaggctc
     1021 acctggagca gcattgggcc agcacagccg cactgattgt ggaacccttg gtgcagggcg
     1081 cagccgggat ggcaatgtac cacccaacgt acctgcagcg ggcgcgggaa ctgtgcaacc
     1141 gctaccaggt ccatttgatc gccgacgaaa tcgccgtggg cttcggccgc accggtacca
     1201 tgttcgcctg cgagcaggcc gggatcaagc cggatttcat ctgcctgtcc aaaggcatca
     1261 ccggcggcta cttgccgctg tccgtggtgc taacgaccga cagcgtgtat gccgcattct
     1321 acgacgaccg gactacacgc ggcttcctgc actcccacag ctacaccggc aacgcgctgg
     1381 cctgccgcgc agcgctggca accctggata ttttcgagca ggacgacgtc atcgtcgcca
     1441 atcgcaccaa ggcaacgcac ttcaacgcgc tgctggcacc gctggcggca catcccaagg
     1501 tgcgcaattt ccgccaccta ggcatgatct gggccttcga ggtggatagc accgatgcgc
     1561 ggttctcgca cggcttttac caagcggcgc tggcgcgtgg cctgctgttg cggccgatca
     1621 acaacacggt gtatctgatg ccgccctatg tgattagtga ggatgaaatg gcgatgctga
     1681 cggaacagtt gctgcaactg gtagagcagg catgaagctg ttagcgctgt tgctggctgg
     1741 ctggctggcg atcacgcccg cgctggccaa cggtctgccg gcgccggtag ccaaggcgct
     1801 gaaagcggct ggcgtaccgc aggacggcgt ggcgttttat gtgcagcagg tggatcagca
     1861 ccagccgcgc ttgagccacc gcgccgatca gccaatgaat ccggcttcga ccatgaagct
     1921 gctgaccact gatgccgggc tggaactgct agggccgtcc taccgctggc gcacggaaat
     1981 ctacggcagc gtgccaccgc aggacgaggt gctgaatggt gacctgatca tcaagggcta
     2041 cggcgatcct gcgttgacac tggaaaattt ctggctgctg ctacgcatgc tcaggcagtc
     2101 cggggtgcgc gacatccgcg gcgacctgct gctggatcgg ggctatttcg acaccagcgg
     2161 caacgagccg cccggcaagt tcgatggcga aacctatcgc gcctataacg ccgcgccgga
     2221 cgccatgctg gtcaatttca aagccacgcg cttcaccttg cgcggtgacc ccgctctggg
     2281 caaggtgctg attaccgccg accccgccct gccgcaactg aacatcgtca accaggtcgc
     2341 cctgagccag gtaccgtgtg ccgactggaa ggaccgcatc ggctaccggg tgctgcgcga
     2401 gaaggatgtg gtgacggtga cgttcagcgg caactactcc gtcgcctgcg gcgagaaggc
     2461 gctggagctg agcatccttg acgatgacgt gtacgttttt gagctgttcc gccgcatgtg
     2521 gcaggagcaa ggcggccaga tcagcggcgg cctgaaaaac ggcagtctgc cgccgggggc
     2581 gcaacggctg gtgcaggccg attcgccgcc gctggccgac gtgatccggc tggtcaacaa
     2641 atacagcaac aacgtgatgg cgcggcagat cctaatgacg ctgggggcgg agcgctatgg
     2701 cgcgccgggc agcccgcaat ccggcgcgct ggcggtgcgc gaatggctgg ccggcaaggg
     2761 catggagtgg ccggagctgg caattgagaa tggggcggga ttgtcacgca acgagcgcat
     2821 cagcgcgcgt catctaggcg agcttttgtt gtctgcctac cggagcccgg tgatgccaga
     2881 gctgatgtcg tcgctaccca tcgtcgccat cgacggcacc atgcagcacc ggctcaagtc
     2941 cagcgccgta gccgggcagg cgcacctcaa gaccggctcg ctggacggcg tgcgtgccat
     3001 ggccggctac ctgctggacg ccagggggcg gcgctgggtg gtggtattca tggccaacca
     3061 tcccgccgca gctggcagca aggccgcgca ggatgccctg ctggagtatg tttacgcgct
     3121 gccatagcac aagcggccaa gcgccggcag accaacttgc ggtatcatgc cgcagatttc
     3181 ccaacccgat ggagactttt caatgaaaca caccagcctt tttgtcggca ctttactgct
     3241 ggcgttggct accgccggct gcaatgccgc cgatccacaa tcccaaacca aggaaaaccc
     3301 tgccatgacc ctgccagtga ccgtgaaaga actaattcag atcgaccaga aagtgggcgg
     3361 cgtcgccgaa gcactgccac tgaacaccgt caaagtgcac tacaccggct ggctatacga
     3421 ccccagcaag ccggatggcc atggcaagaa gttcgacagt tcgctggatc acggcgaggc
     3481 attcgagttc atgctgggcg gcggccaggt gatccgcggc tgggatgaag gcgtggccgg
     3541 catgaaggtg ggcggcaagc gcaccctgat tatcccccag cacatgggct acggcgcgcg
     3601 cggcgccggc ggcgcgattc cgccttatgc cacgctgatc ttcgatgtgg aactgctgga
     3661 cgtacgctag caggagcggc gcctcgccgc gatgctgtag aagggggagt cgcgccgggg
     3721 ggggtggcgc tcctacaggg ggaaaaatcc ccccgttaca acccgagctg cggccacagc
     3781 gcatcgattc ttgatttcac cgcctcttcc atcgcgatgg ggcggcccca ttcccggctg
     3841 gtttcgcctt tccacttgtt cgtcgcgtct atccccatct tgctgccaag gccggaaacc
     3901 ggactggcga aatccagata gtcgatcggc gtgttttcca cgattacggt gtcgcgcgcg
     3961 gcgtcgaccc tggttgtgat cgcccacacc acttccttcc agtcgcggat attcacgtcg
     4021 tcgtcggtga cgatgatgaa cttggtatac atgaactgac gcagaaaact ccacaccccc
     4081 atcatcacgc gcctggcgtg gccggaatac tgcttcttca tgctcaccac cgccagccgg
     4141 taactgcacc cttccggcgg caggtagaag tccgtgatct ccgggaactg cttttgcagc
     4201 agcggcacga agacttcatt gagtgccaca cccagcaccg ccggctcatc cggcggcttg
     4261 ccggtgtagg tgctgtggta gatggggtcg cgacgcaagg caatgtgggt gatggtgaac
     4321 acgggaaact tgcccacctc gttgtaatag ccggtatggt caccgaacgg gccttctggc
     4381 gcggtttctc ctggcgcaat atggccctcc agcacgattt ccgcgccggc cggcacctgc
     4441 aagtcactac tcacacactg cacgatttcg ctcttgccgc ctcgcagcag gccggcgaac
     4501 tggtattccg acagcgtatc cggcactggc gtcaccgcac ccaggatggt ggcagggtcg
     4561 gcgcccagtg ctacggcgac cgggaatggt tcgcccggat gcgccgcaca ccagtcgcgg
     4621 aagtccagcg cgccgccgcg atgcggcaac cagcgcatga tgaccttgtt tttggcgatt
     4681 acctgctgcc ggtagatgcc cagattctgc cgctccttca gcggccccct ggtcaccgtc
     4741 agcccccagg tgatgagcgg cgccgcgtcg cccggccagc aggtctggat cggcagcctg
     4801 gacaaatcca cctcgtcacc ctcccacacc acttcctggc aaggggcgcg gctgacgatt
     4861 ttcggtgcca tgtccagcac ctttttgaac ttgggccact tgtcccaggc gtcgcgcagc
     4921 cctttaggcg gttccggctc cttgaggaaa gccagcagct tgcccacttc gcgcagcgcc
     4981 tccacgcttt cctcgcccat gcccagcgcc acccggtgcg gggtgccgaa caggttgccc
     5041 agcaccggca tggagtggcc aatcacgttc tcgaacagta tggccgggcc gcccgccttg
     5101 agcacgcggt cgcagacttc cgtcatttcc agttggggac tgaccggcac gcgtacgcgg
     5161 cgcagttcgc cctgggcttc aagctggctg atgaagtcgc gcaagtcctt gtattgcatg
     5221 gcatggcatc cggatgattt acgcgacagt ttacacagga agtccttgcc agcgcacgat
     5281 ttcggctaaa atcagcgctt tgcagatcga tgaggaaaca caatgcaagc acgggtgaag
     5341 tgggtcgaaa acgtatgttt cgtgggagag tcggaaaccg gccatgcgct ggtcatggac
     5401 ggtgcgccgg agtccggcgg gcgcaacctc ggcatgcgcc ccatggaact gctgctgatc
     5461 ggcatgggcg gctgcacctc gttcgacgtc gtgacgatcc tgaagaaggg gcgccagcag
     5521 atcaccgact gctacgtgga actgaaggcg gagcgtgcgg aaacggtgcc caaggtattc
     5581 acgaaaatcc acgcccactt cgtcgtcacg ggtcgcggac tcaatccggc gcaggtggag
     5641 cgcgccatca acctgtcggc ggagaaatac tgctcggcct cgctgatgct cggcaagacc
     5701 gccgagatca cgcacgattt cgagattgtc gaggcgtagc ctcaagagag aaatctaggc
     5761 tgggcagggt ttatctgccc accctacgcc tgcatcacat ttgcaaggta agacgaggct
     5821 atcatttcat tgcgtgcgcg gcgattggcg tactgagaat aacaaacaca tcgtcggatg
     5881 tttcagatcg ttcgaccttg agaattgggt agactctact aaggtctgca tgcttggccc
     5941 agacgcttgg gcctcttaag cctgtgggtg gggcagagag gactgcatag cccctaccac
     6001 cagcctcaag agtttcaatt gaccgcttac gtcctgcgta gccgacacgc tgagtatctc
     6061 ccttgatggc gtgactttcc gcatcgaaac acctgacgcg aagatatgcg ctaggcagag
     6121 cgtgctctcg aatccgctgc ccgggtgcct gccagagttg catgagtact gatcctgcct
     6181 catttataga gtcccaagaa ttggccaccg acccataggg tttgaagccc gttgaacgca
     6241 aaaattctgt cacagtcata ggtggttctc ctcagcaagt aatgcgccaa acgtccaacg
     6301 ttctctatat atcaccctca agccaacctc acccccagcg cttctccttt ctccaccagc
     6361 acaaacaacc gatcaatatt gggatgctta ccaggattca gccgagcatc ctcggtatgc
     6421 tcggcataaa gctcgcagcc aacccgcgcg gcttccgggg tgatgctgcc gaactgctgg
     6481 gcgaggtgca ggtagaggcg tagggagcct tggctgccgg gtttgttttc gatggtgccg
     6541 acgaggttgc cggcggcgtc gagcagttcc agtttggcga taccgtcgat cgacgggaag
     6601 gtttccaggt tttccttgaa gctgggcatg gcgggttgtt ccttggttgt agcgcagagc
     6661 ctgcccttga gtgcttgaat cgggggcggc ctcgcctgct ggttttaccc ctcaccccaa
     6721 ccctctccca caaggagaga ggtggagcgc ggttttaatc atatccaata ggcggttttc
     6781 gtcagtattt ttgaagtgag tttcatagcc atcttcaccg gcagcggcag ttcggcgccg
     6841 ccgtggtgca gggcggtggt ggcgtgtttg gcttcgtcga ttttcatttg ttcgaggatg
     6901 gcgcggctct tttcgtcctg cggcggcagg ctctgcaggt ggccttcgat atgcttgtcg
     6961 acctggttct cggtctcggc cagaaagccc aggttccatt tatcgcccaa cgcaccggcc
     7021 agcgcaccga gggcaaaaga gccggcgtac cacagcgggt tgagcaggct cttgtggctg
     7081 cccagctcgt aaatgcgctt ctcgcaccag gccagatgct cggtttcttc ctgcgaagcc
     7141 tcgcgcagcg cggcttccgt ggcggggttg cgcgccgtca gcgcctggcc gttgtagagc
     7201 gcctgggcgc agatctcgcc gctgtggttg acgcgcatca acgcggcagc gtggcgcttt
     7261 tcctgctcgc tgagcgggga ctcgacctga tcgcggtccg gaaacggacg cacgctctgt
     7321 gcctgtgcca gcacggtgcg caggcctttg tcgaactcga taatcagtcg atcaagcatt
     7381 tttccatccc aattgtctgg cgagtatcac cacagggtgc gccacctcga cctttatgcc
     7441 gtcatcccgc agtccggcgg cgatgtgcag ggcacagccg atgttggagg ttgccagcag
     7501 cgtcgcgctc gatgcacggc aggcgctcat tttatcatcg cgcagttgtc gtgccatcgc
     7561 cggctgggtg agcatgtagg cgcccgcccc gccgcagcat tgggtattgc ctggcaaggg
     7621 tttgacttcg gcgccgggaa tgcgccgcaa caggctgtag acttcctcgt gctggcgcat
     7681 ggtgttgcgc agcgaacaag catcgtgcac gtggatggtg gcttccagtt gggccggctc
     7741 gatcccattc cagttcgcct gtcccaggaa cgcggcgatg tcggtggcac gcttgccgaa
     7801 gtggtcacgc agcccggcgc cgcagccgct gacgacgctg atcaggggca tgtcggccag
     7861 tgcggcgaac gcgcgcgcat ttttttccag catgcgtgcc gaggcggcgg catctcccga
     7921 ttgccgcgcg atgccgccgc agcagcattg ctccgcaggg acatggacgg tataaccgag
     7981 ccggttgagc acgaagatgg tagcgcgcag cgtatcggcg tcagcgatgg ccgaaatgca
     8041 gccgaggaac agcgccactt cgccgcgcac ctgctcggct gggtagacgc tgcgccagtt
     8101 ttcctgtgcc gggatggccg gcagcagcgc atcggcctgc cggagttttg gcagcattgc
     8161 ggccgccaga agccgcaaac cgctgatctg agcgaaacgc aatgcgtgac cggcaagcga
     8221 caacatggcc cggcgttcca gcagccatgt tactgcacgc tgtatcaacg tggctggcaa
     8281 agcaatgatg gcgcgcgctg catcgaccag tgtgccgtag gcgacgccat tggggcagac
     8341 gttttcgcag gcacggcagg acaggcacag gtcgatgtgc tgcacaaaac gcttgctggc
     8401 cggcaagcgg ccctgcgcca cggcgcttat gagttggatg cggccgcgcg gcgagtcggc
     8461 ttcggactgg gttttgcgat aggtgggaca atgcggcagg cacaggccac aggcgacgca
     8521 acggtctgct tccttggcca gtttggagag atgtgaaatg gattcggcgt tcatgcaaac
     8581 gggcggctga cgccgcccgg ctatcgcatt tacttcttct gcagaatctg cagacccttc
     8641 agcaggttca tcgcctgcgt gaactggtag tcgctcttgg tacccggttc catcggcgca
     8701 tttttgtcgc cactcttgtc attctcatct ccggcgggtg gcgcgatgac cttggccggt
     8761 tgctcggggg cgctgggctt ttcgccatcc ctggggttgg acaggtggcg ggtcaggtcg
     8821 gcctcgcgga aattcattcc ctgctcggcg ttgttgaccg tagcctcttc cgcctggatg
     8881 tcggggacga tgcccttggc ctggatggaa cggccattgg gcgtgaagta gcgcgccgtg
     8941 gtgagcttga tggcggtacc gttgttcatt ggaaggatgg tttgcaccga acccttgccg
     9001 aagctctggg tgccgagaat gatggcgcgc ttgtgatcct gcagggcgcc ggcaacgatt
     9061 tcggaggcag aagcggagcc gccattgatg agcacgacca tcggcacggt tttcaactcg
     9121 ggcggcagtc ccttgatgta atcctcggct gggccgctgc gcacgtagtt ttccgggctg
     9181 gcagtgaggc gcatcttggc atccggcacg cggccctcgg tatagaccac cagttcgcct
     9241 ttgggaatga aggcggccga caccccgacc gcggcattga ggaggccgcc agggttgttg
     9301 cgcaggtcga gcacgacgcc tttcaggccg tccttgttct cgtcgaccat ggccttgagc
     9361 gccttggcca gatcggcacc cgtgtgctcc tgaaactggg tgatgcgcac atagccgtaa
     9421 cccggctcga cggatggctt gctcttgacg ctttgcacct tgacaatggc acgagtgagc
     9481 gtgaccacga taggtttgtt ttcgcccttg cgcaggatcg tcagtacgat tttcgtgtct
     9541 ggcttgccgc gcatgagctt gaccgcctcg gtcagcgtca tgcccttgac cggtgtttcg
     9601 tcaagcttga tgatgaggtc gcctggtttc aggccggcct tgtaggctgg ggtgtcctcg
     9661 atcggcgata ccaccttgac gaagccatct tccatgccga cctcgatgcc aaggccgccg
     9721 aattcgccct gcgtgccggc ctgcaagtcc ttgaaggcat cctcatccag ataggcggag
     9781 tgcggatcga ggcccgacag catgccgctg atggcctcgg aaataagttt cttatcctcc
     9841 accggctcga cgtaatcact cttgaccttg ccgaacactt cggcaaatgc gcgcagatcc
     9901 tccagcggca gctgggtctt ggcgtcctta tcggcgatgg cggagtaatt gagactcagc
     9961 atgatgccga gaattgcgcc gacgccaatc aagccgaatt tttcgaattt agcgcgcatt
    10021 gtcacgggtt ccttaaaacg cttgtggagt ctgtgtggga cagcaaattt tacaaagcgt
    10081 ttattttccg ctatttggca cgctcatgca aagccaaaag acaggcttgc cgatttcatc
    10141 gagaaacaag cctgccccca ttccgatcgt cagaactcgt attcggcctg cattctgaag
    10201 ttgttgtcca acgacgcact ggtggtcccg aacagccacg cagcgttcca cttgagggcc
    10261 tgcttgtccc caaggtttat tttgccgaac gccgctggtc caaggcggtg gctttgcgca
    10321 ttggaatttt cccagtggtt ccattcgccc atgtcaccga agccctgggc accaaattca
    10381 aaggctggct gccagcggta tttgacttgc cactggtagc ccatttcggt gttgtagctc
    10441 tcgccatcgt tggggccgaa cttgcgctcg aacagcaggt tgccgttcaa ttgcactctg
    10501 cctacatcgg tttgcaacag cggccctagc ttgaattcgt aaggtgtgtc gcggcgatca
    10561 ctgaagggga actctatttc cgtaatcagt ccgagttcga acgggtattt tccggtttcg
    10621 atcaactgga acttgttttc ccactctccc aacgtcaggc gctcgctacc ttcgcgctcg
    10681 cgcttcagat aaatttcgct aaaccagtac tccgtcgcac catagccaaa gcccagcatt
    10741 gacacctgct tgcgctcatc gtccggccgc tgttcggccg tgccgtactt gaattcgatt
    10801 tcccgctcac cgtattccac ggtgggcata tagacatagt cagccggacc ggcggcgacg
    10861 atgccggaat aaagggcgcc taatatcagc gccaatcttg ttcttttcat gatgcaactg
    10921 ctccttgaga ggtggatttc gaaattcgtt tgttccattt catgcccagg ccgatggcaa
    10981 tcagtgtgat gacataaaaa actatctgca tgccggtcgg acgcgcgtcg taaccggtca
    11041 gactgtgcag cagcatccct gcaacggaat g
//
LOCUS       DAOMFA010000044        10815 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_630_length_10815_cov_4.704926, whole genome shotgun sequence.
ACCESSION   DAOMFA010000044 DAOMFA010000000
VERSION     DAOMFA010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 10815)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10815)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10815
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_630_length_10815_cov_4.704926"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(111..908)
                     /locus_tag="PKW44_03595"
     CDS             complement(111..908)
                     /locus_tag="PKW44_03595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR50701.1"
                     /translation="MLAALGLIAPAPALAEDEGEASNEAEIAKKTQNPIADLISLPLQ
                     LNYDHDIGPQEDGKTWYLKVQPVIPISLNEDWNVISRTIAPIQYQSDYAPGAGDHTGV
                     GDITQSFFFSPKKLTESGWTWGVGPALLIPSGNEDMSGEKWGAGPTGVALKQRDGWTY
                     GILANQIWSYAGSDLHPDVNSTFLQPFLTYTNSHYTSFGINSESTYDREAREWSVPIN
                     LYVNQLVKIDKQLISLQAGVRYWADTPDNAGPENWGLRLGATFLFPK"
     gene            complement(974..2437)
                     /locus_tag="PKW44_03600"
     CDS             complement(974..2437)
                     /locus_tag="PKW44_03600"
                     /inference="COORDINATES: protein motif:HMM:NF018561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1254 domain-containing protein"
                     /protein_id="HQR50702.1"
                     /translation="MKYSTLKTLGNLLACFLVIALLAGCSKNDSISQAEKADAAKPGI
                     AETKAIAEEAFIYGLPIVMNYAVMHEFCVDKNSGQYKAPFNTISNEARVFTYKDTAVV
                     TPNSDTPYSMLWLDLRAEPMVISVPAVEKARYYSVQLTDGNAYNYGYIGSRATGNDAG
                     DYLVAGPDWKGDAPAGIKKVFHSTTPFALTIFRTQLFNAEDMPNVVKVQAGYKAQPLS
                     AFLKQPAPPAAPKIDFLPATTAGIKANFYQYLDAALQYVPATPENKDILARLASIGIG
                     AGKKFDFKDLSAEHKAAVLLAMKEGDDQVDKYLATGMKNINGWKVGSIFGDSAFYNGN
                     WLMRAAAAKGGIFGNDAVEAMYPMTRVDTTGETLDGSKHNYTLTFPAGQFPPVNAFWS
                     VTMYDGKTQLLIENPINRYLINSPMLPKMKMNTDGSLTLYIQKDSPGKDKEANWLPAP
                     NGPIYLVMRLYWPKTEAPSILPPGEGTWQLPSLVQAK"
     gene            complement(2487..4877)
                     /locus_tag="PKW44_03605"
     CDS             complement(2487..4877)
                     /locus_tag="PKW44_03605"
                     /inference="COORDINATES: protein motif:HMM:NF024821.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQR50703.1"
                     /translation="MAKRSNKRSGGRTASASLPAAANADGKLPGNRRRVGICLAAVLA
                     VLVLGGLGFHFFPAENKTALPASKIVTGSSLPAAQFVGGKKCAQCHAAEYQAWRGSYH
                     DLAMQDASAETVLGNFNQARFRGDGMDVSFFQRDGKYLIRAEGPDGKPGDFEVKYTFG
                     QFPLQQYLLELPEGKLQAFTVAWDSRPKEKGGQRWFHLYPGQKFSYNDELHWSGVQQN
                     WNFMCADCHSTNLQKNYDPARQRFNTTWSEVNVSCEACHGPGSAHVAWADAGGKGGAP
                     DKGLTAALDERKGIAWNHDATTGNATRSAVRKSGHEIEVCAQCHSRRGQIAEGYHAGR
                     AFLDYYRPSLPGEPLYFADGQQKDEVYTWGSFLQSRMHARGVTCSDCHQPHTLKLRAQ
                     GNAVCAQCHAAEKYDGAQHHFHKLEGKGALCVSCHMPERLYMVIDGRHDHSLRVPRPD
                     ITVAQGADKAPNACNNCHRDHDAKWAADAVAKWYGPNRRQEQTFGGTLASAGRLDMED
                     VQALVTLTDNNGLPPIARATALQALAAQPGPAMQQAVRGHAQDGDELVRLAAAEAAVA
                     LPPAERAAAVLPLLHDERLAIRLAAVNGLLDFPRDSFSPERLAELDAGIAEYRRVQVY
                     NADRAEGQANLGQLEARLGNAEAATAAYEAAIRINPAFVPAYVNLADLQAHQGNEAGA
                     EKTLRRALAVEPENAAAHHALGLSLIRGKRLKEAVSELRRAAVLRPEDARYAYVYAVA
                     LYETGDPKGAIRILKQAAARHPENLDILGALVQYAAKSGDSDTAAEYAIKLKAMQG"
     gene            complement(4945..5493)
                     /locus_tag="PKW44_03610"
     CDS             complement(4945..5493)
                     /locus_tag="PKW44_03610"
                     /inference="COORDINATES: protein motif:HMM:NF016269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding SYLF domain-containing protein"
                     /protein_id="HQR50704.1"
                     /translation="MFKRMSVLLSGLAMAACLVSFPAAAVDNAKLDAEAHKALQDLYK
                     KEPKAAELGKKAKGILVFPQIVRAGFIIGGEGGDGTLLVGGKPAGHYSDGGLSVGLQV
                     GGQEFGYAMFFMDDEALKVLSSADGWSIGVGPTVVVVDKGVAKNLTTKTAASGVYAFI
                     FSQEGLMAGMALKGNKITKIKE"
     gene            complement(6073..8550)
                     /locus_tag="PKW44_03615"
     CDS             complement(6073..8550)
                     /locus_tag="PKW44_03615"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HQR50705.1"
                     /translation="MTKPRKPKSPRQHYLNCDEHSIEKSLANHLIFTGGKTEHYATSR
                     DWYDTVAHTVRDHLIERWVDTVGTYDKQDPKRVYYLSLEFLIGRTLSNAAHNLDIEQP
                     VRAALKDLGQDMEHVEEMETDAALGNGGLGRLAACFLDSMATLDLPGLGYGIRYEYGM
                     FHQRIEDGKQVEHPDNWLRYGNPWEFQRPERLYPVHFNGHVVQFPDGHGGIEHHWVET
                     DTVMAMAYDVPIPGFKTETVNNLRLWSAKATREFNLESFNVGDYVGAVEEKNTTETLS
                     KVLYPNDTSVMGRELRLRQQYFFVSASIQDILHRFFAGKHDLKSLPEKIAIQLNDTHP
                     AIAVAELMHQLLDVQRVKWEQAWNLTTRIFAYTNHTLMPEALETWPVEMFGRVLPRHL
                     EIIYEINHRFLHDVNHRFPGDPELLKRVSIIDESHGRRVRMAHLAVVGSHTVNGVAAL
                     HSELLKTTLFADFNRIFPGRFINVTNGITPRRWLNQCNTGLTELIKSRIGDGFVRDLA
                     QLKKLAPYADDAGFRKQFLEVKRANKLRLAKRIEQQTGVHINPDSLFDVQVKRIHEYK
                     RQLLNVLHVITLYNRIRTGKTEGLVPRTVIFAGKAAPGYWMAKQIIRLINDVASLVNN
                     DPAVGDLLKVVFLPNYDVSSAEKIFPASELSEQISTAGTEASGTGNMKMALNGALTIG
                     TLDGANIEIGEEVGDENIFIFGLTTQEVTDLRARGYNPWEYYNGNPELRQALDMIGNG
                     FFSMDEPERYRAIYENLTWNGDHYLLLADYASYIACQDHVNALYRDQDEWARRAILNV
                     AHMGKFSSDRTIQEYADKVWGVKGVKR"
     gene            complement(8657..9106)
                     /locus_tag="PKW44_03620"
     CDS             complement(8657..9106)
                     /locus_tag="PKW44_03620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR50706.1"
                     /translation="MRMKGYPGWFYRTLLATTGALFVSGSLLIPTALEMKLEWEVPWR
                     LTGDPQIAMAALHASAAFLAAAVVGALWSIHMRAGWKKRKNHRTGIALLASMLLLGIT
                     AIGIYYLGEDQAALCASVAHMLIGVLLLLFLAVHIVAGWRVRSQHQH"
     gene            complement(9097..9909)
                     /locus_tag="PKW44_03625"
     CDS             complement(9097..9909)
                     /locus_tag="PKW44_03625"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HQR50707.1"
                     /translation="MKRMRPLLGTFVEIGVQSGAGAERALDAAFDAVAEVQGLMSFHD
                     PHSDLSRLNQAGGGEVALHPQIVRVLRLARAMTQASAGLFNCTVGGALVRRGVLPDHG
                     GRAPLDCGKAGDIEIRGTQVRLRRPVRVTLDGIAKGYAVDCAIRAMQRHGAGAGWVNA
                     GGDLRAFGDLALPVHRREMDGSLRPMGAIMDAAIATSCVRVVHDQAYPAWIVGESRAP
                     QVGVWSVMAHSAWRADALTKVAGLADDGERAGLIERLGGRWVSHADGVAACA"
     gene            complement(9911..10456)
                     /locus_tag="PKW44_03630"
     CDS             complement(9911..10456)
                     /locus_tag="PKW44_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HQR50708.1"
                     /translation="MHKSNMNGLWLLPMAAIVAPAYAQDYLTAAQAQKSLFPDADAFQ
                     PMLVKLGEAQRDKIKALSGLRQRWEEQKVWRVEKDGKQLGWFIVDEVVGKHEFITYGV
                     GLSLDGHVVGIEVMTYRETKGDQIREAGWRKNLVGKTLADPFKLDEDVPNISGATLSC
                     RNVMDGVKRLLALQKVALASD"
     gene            complement(10467..>10815)
                     /locus_tag="PKW44_03635"
     CDS             complement(10467..>10815)
                     /locus_tag="PKW44_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR50709.1"
                     /translation="SSMASLPPDYEWPTDPEMEIELTLGTGLSYRFMPNWFIGGELQY
                     QTEYETQVGQERWSLFAGPNLHYGGEKFWVTATWFPQIRGGGLTYPGQNDHDLQLIEK
                     TEQEFRVKFGIDF"
BASE COUNT         2002 a         3323 c         3207 g         2283 t
ORIGIN      
        1 ccggcgcgat caacagataa aaatcgcgcc gggggcggcg ctcctgcaaa gggattttcc
       61 ctaatggaca atccgggact aagcatcttt aacgttatca gatgactata tcatttcggg
      121 aacaaaaaag tcgcacccag gcgcaacccc cagttctcgg gaccagcgtt atccggggtg
      181 tcagcccagt agcgcacgcc ggcctgcagg cttatcaact gcttgtcgat tttgaccaat
      241 tggttgacgt acaggttaat tggcaccgac cactcgcgtg cctcacggtc ataagtagat
      301 tcagagttga taccaaagct ggtgtagtgg ctgttggtat aagtcagaaa cggctgcaaa
      361 aaggtggagt tcacgtccgg atggaggtca ctgccggcgt aagaccagat ctgattggcc
      421 aaaatgccgt aagtccagcc gtcgcgctgt ttgagcgcaa ccccggtagg gccggcgccc
      481 catttctccc cgctcatatc ttcattgccg ctggggatca gcagcgccgg gcccacgccc
      541 caggtccagc cggattcggt aagcttcttc ggtgaaaaga aaaagctctg cgtgatatcg
      601 ccgacaccgg tgtgatcacc ggcgccgggc gcataatcgc tttgatactg gatcggcgcg
      661 atggtgcgcg aaatgacgtt ccagtcctcg ttcaggctga tggggatgac cggctgcacc
      721 ttgagatacc aggtcttgcc gtcttcttgc ggaccgatgt catggtcgta gttgagctgc
      781 aagggcaggc tgatcaggtc ggcgatcgga ttctgtgttt tcttggcgat ctcggcttcg
      841 ttgctggcct cgccctcatc ctctgcaagg gccggagcgg gtgcaatcag tcctaatgcc
      901 gccagcacgg cgacgaggtt ttgtttgtaa ttagttttca ttgcaacgcc ttttcgatga
      961 ttgcctggcg tggttacttc gcctgcacga ggctgggtag ttgccaggtg ccttcaccgg
     1021 gcggaaggat ggaaggcgct tcggttttcg gccagtagag gcgcatgacg agatagatcg
     1081 ggccgttagg cgcgggcagc cagttcgctt ccttgtcctt gcccggcgaa tctttttgta
     1141 tatagagcgt cagcgagccg tccgtgttca tcttcatctt tggcagcatc ggcgaattga
     1201 tcaggtagcg gttgatcggg ttttcgatca gcaactgggt tttgccgtcg tacatcgtca
     1261 ccgaccagaa agcattgacc ggtgggaact gcccggcggg gaaggtgagc gtgtagttgt
     1321 gcttgctgcc gtcaagcgtc tcgccggttg tgtccacgcg cgtcatcgga tacatggcct
     1381 caaccgcgtc gttaccgaag atgccgccct tggcagcagc agcgcgcatc agccagttgc
     1441 cattgtagaa agcgctgtcg ccaaatatcg aacccacttt ccagccgttg atgtttttca
     1501 ttccggtggc cagatacttg tccacctgat cgtcgccctc cttcatcgcc agcagaacgg
     1561 cggccttgtg ttccgccgag aggtccttga agtcaaactt cttgcccgcc ccgatgccga
     1621 tgctggccag cctggcaagg atgtccttgt tctccggcgt cgcaggcaca tactgaaggg
     1681 cggcgtcgag gtactggtag aagttcgcct tgattcctgc tgtcgtcgcc ggaaggaaat
     1741 cgatcttcgg tgcggcgggc ggcgcgggtt gtttcagaaa cgcggaaagc ggctgcgcct
     1801 tgtagcccgc ctgcaccttg actacgttag gcatatcctc ggcattgaag agctgggtgc
     1861 ggaaaatggt caacgcgaag ggtgtcgtcg agtgaaacac cttcttgatg ccggcgggcg
     1921 catcgccttt ccagtccggg ccggctacca ggtagtcgcc cgcatcgtta ccagtcgcac
     1981 ggctgccgat atagccataa ttgtaggcat tgccatcggt caactgcacc gagtagtagc
     2041 gtgctttttc caccgccggc accgagatca ccataggctc ggcgcgcaaa tccagccaga
     2101 gcatcgaata tggcgtgtcg ctgttaggcg tgacgacggc ggtgtccttg taggtgaaga
     2161 cgcgtgcttc gttgctgatg gtgttgaacg gtgccttgta ttgccccgag ttcttgtcca
     2221 cgcagaactc atgcatcacc gcgtaattca tcacgatcgg caagccgtag atgaatgcct
     2281 cctcggcaat ggccttggtt tcggcgatgc cgggcttggc cgcgtccgcc ttctccgcct
     2341 gcgagatgga atcgttcttg ctgcagccgg ctaacagcgc gatgaccagg aaacatgcaa
     2401 gcagattccc gagcgtttta agcgtcgaat atttcatgat gtatctcttc ctttctcatt
     2461 taatggggga tttgtttttt aaaccttcag ccctgcattg ccttgagctt gatcgcgtat
     2521 tctgccgccg tgtcgctgtc gccggatttc gccgcatact gcaccagcgc ccccagaata
     2581 tcaaggttct ccggatgacg cgcggccgcc tgtttcagta tcctgatcgc ccctttcgga
     2641 tcgccggttt cgtacagcgc caccgcgtaa acataggcat agcgcgcatc ttccggccgc
     2701 agcacggcgg cgcgccgcaa ctctgaaacc gcttctttca aacgcttgcc gcggatcagc
     2761 gacagcccca gggcatggtg cgcggcggcg ttctccggct cgacggcgag ggcgcggcgc
     2821 agggtttttt ccgccccggc ctcgttcccc tgatgcgcct ggaggtcggc caggttgacg
     2881 taggcgggca cgaaggccgg gttgatgcgg atggcggctt cataggcggc cgttgcggct
     2941 tctgcgttgc ccagccgggc ttccagttga ccgagattgg cctgcccctc ggcgcggtcg
     3001 gcgttgtaga cctgcacacg ccggtattcg gcgatgcctg catcaagctc cgccagccgc
     3061 tccgggctga aactgtcgcg cgggaagtcc agcaagccat tgaccgccgc caggcggatc
     3121 gccagccgct cgtcgtgcag caagggcagc acagcggcgg cgcgctcggc ggggggtaac
     3181 gcgacagcag cctcggcggc ggccaggcgt accagttcgt cgccgtcctg ggcatggcca
     3241 cgcactgcct gctgcatcgc cggccccggc tgggcagcca atgcctgcaa cgcagtggcg
     3301 cgggcgatgg gcggcaagcc attgttatcg gtcagcgtca ccagcgcctg gacatcctcc
     3361 atatccagcc tgccggcgct cgccagcgtg ccgccgaagg tctgctcctg gcggcggttg
     3421 gggccgtacc atttggccac ggcgtccgcc gcccatttgg catcgtggtc gcggtggcag
     3481 ttgttgcagg cattgggcgc cttgtccgcc ccctgcgcca ccgtgatgtc gggccgcggc
     3541 acccgcaggc tatggtcatg gcggccgtcg atcaccatat acagccgctc cggcatgtgg
     3601 caggaaacgc acagcgcacc cttgccttcc agtttgtgga aatggtgctg ggcgccgtcg
     3661 tacttttccg ccgcgtggca ttgcgcacaa accgcgttac cctgcgcgcg cagcttcagg
     3721 gtatgtggct ggtggcagtc ggaacaggtg acgcccctgg cgtgcatgcg gctctgcagg
     3781 aaggagcccc aggtgtagac ctcatccttc tgctggccgt cggcgaaata cagcggctcg
     3841 ccgggcaaac tgggccggta gtagtcgaga aaggcacggc cggcatgata gccctcggcg
     3901 atctgcccgc gccgcgaatg gcactgggcg cagacttcga tctcgtgtcc gcttttgcgc
     3961 acggcgctgc gggtggcatt gccggtggtg gcatcatggt tccaggcgat gcctttacgt
     4021 tcatcaagag cggcggtgag tcccttgtcg ggcgccccgc ccttgccgcc cgcatcggcc
     4081 cacgccacat gcgccgaccc cggcccgtgg caggcctcgc agctgacgtt cacttccgac
     4141 caggtggtat tgaaacgctg tctggccggg tcgtaattct tttgcaggtt ggtggaatgg
     4201 caatcggcgc acatgaagtt ccagttctgc tgtacgccgc tccagtgcag ttcgtcgttg
     4261 tagctgaact tctggccggg atagaggtgg aaccagcgct gcccgccctt ttccttcggc
     4321 cggctatccc aggccacagt gaaggcttgc agcttgcctt ccggcagttc gagcagatac
     4381 tgctgcaacg ggaactggcc gaaagtgtat ttgacctcga agtcgccggg cttgccgtcc
     4441 ggcccctcgg cacggatcaa atattttccg tcgcgctgga agaagctgac gtccatgccg
     4501 tcgccacgga agcgcgcctg gttaaaatta cccagtaccg tttccgcact cgcgtcctgc
     4561 atcgccaggt cgtggtagga gccgcgccag gcctggtatt ctgcggcatg gcactgcgcg
     4621 cattttttgc cgccgacaaa ctgcgcggca ggcagcgacg aaccggtgac gattttcgat
     4681 gccgggagcg ctgtcttgtt ttccgcaggg aaaaaatgga accccaagcc acccagcacc
     4741 agcacggcaa gcacggcggc gaggcagatg ccgacacggc gtcgattgcc tggcagtttg
     4801 ccgtcggcat tcgcggcggc gggaagcgaa gcggaggccg tgcggcctcc gcttcgtttg
     4861 ttgcttcttt tagccataac atccgctcac aagaaaaccg gacagggcgg acaagactag
     4921 cttcctctca tccaccctga tgtgttactc cttgatcttg gtgatcttgt tgcccttcag
     4981 cgccatgccg gccatcaggc cttcctggct gaagatgaag gcgtaaacac cggaagcagc
     5041 ggttttggta gtcaggttct tggcaactcc cttgtccacc accaccacgg tcggacccac
     5101 gccgatggac cagccatctg cgctactcag cactttaagc gcttcgtcat ccatgaagaa
     5161 catggcgtag ccaaactcct gaccaccaac ctgcaggccg accgacaggc caccatcgct
     5221 gtagtgtccc gccggcttgc cgccgaccag cagcgtaccg tcaccgcctt cgccaccgat
     5281 aatgaacccg gcgcggacga tctgcgggaa caccaggatg cccttggcct tcttgcccaa
     5341 ctcggcggcc ttgggttcct tcttgtaaag atcctgcagc gccttatgcg cttccgcgtc
     5401 aagcttggcg ttgtccaccg cggcagcggg gaaagaaacc aggcaagccg ccatcgccag
     5461 cccactcaac aatacggaca tgcgtttaaa cattttcagt cctcagtaaa gttaatagga
     5521 ttacctgtcc tgcaacttct gatacaaccg gaacacacgc taatttacct cttgggaaga
     5581 tggcactcca atcatttttc atgtcgatga cagtccagcc ctgcttctgt atttgctggc
     5641 agattcaatc cattaaactc ttattggatt tcatcgtatt tccttttttt tattacgatt
     5701 gacagcagtg ccacaactca aaactgcggc acctcacggc tcccaaaagc gtctaatcca
     5761 atccagcacc tttgagcttt tatgcaggag aaggaggatt ctaatcccct tgggtattcg
     5821 aatgaaagta gtactttgat gaaaaacgtg gggctgcggc atgatgacgc acatgtcacc
     5881 aacccccgtg ggcggcctcc tgccagtcca ttagcaaccc tccgcgcgcc atgccaaaga
     5941 ggctgcacgg gatgggcaag gccatgaggg atgtcaaaaa aaaacagccc ccgattttag
     6001 cgggggctgc gagacacttt gggtgtggcg cgccgcgcga tgcggcagcc actccatctg
     6061 atcaggactt catcaccgct tcacgccctt cacgccccaa accttgtcgg cgtattcctg
     6121 gatggtacgg tcgctggaga acttgcccat atgcgccacg ttgaggatgg cgcggcgcgc
     6181 ccactcgtcc tggtcgcggt agagggcatt aacgtggtcc tggcaggcga tgtagctggc
     6241 gtagtcagcc agcagcaggt agtggtcgcc gttccaggtc aggttctcgt agatggcgcg
     6301 gtaacgctct ggctcgtcca tggagaagaa gccgttgccg atcatgtcca gcgcctgccg
     6361 cagctcgggg ttgccgttgt agtattccca cgggttgtag ccgcgggcgc gcaggtcggt
     6421 gacttcctga gtcgtcaggc cgaagatgaa gatattttca tcgcccacct cttcgccgat
     6481 ctcgatgttg gcgccgtcca gcgtgccgat ggtcaacgcg ccgttgagcg ccatcttcat
     6541 gttgccggtg ccggaggctt cggtacctgc ggtggagatc tgctcggata gttcgctggc
     6601 cgggaaaatc ttttccgccg aggaaacatc gtagttgggc aggaacacca ccttgagcag
     6661 gtcacccacg gccgggtcgt tgttgaccag gctggcaacg tcgttgatga gacggatgat
     6721 ctgcttggcc atccagtagc cgggcgccgc cttgccggcg aagatcacgg tacgcggcac
     6781 cagcccttcg gttttgccag tgcggatgcg gttgtacagg gtgatcacgt gcagcacgtt
     6841 gagcagctgg cgcttgtatt cgtggatgcg cttgacctgc acgtcgaaca gcgaatccgg
     6901 gttgatatgc accccggtct gctgctcgat gcgcttggcc aggcgcagct tgttggcacg
     6961 cttgacttca aggaactgct tgcggaaccc ggcatcatcg gcatagggcg ccagcttctt
     7021 gagctgtgcc aggtcacgca cgaagccatc gccgatgcgc gacttgatca gctcggtcag
     7081 cccggtgttg cactggttga gccagcggcg cggcgtaatg ccgttggtaa cgttgatgaa
     7141 acgcccaggg aaaatgcggt tgaaatcggc gaacagcgtc gtcttcagca gctcgctgtg
     7201 caacgccgcc acgccattga cggtgtggct gccgaccacg gccaggtgtg ccatgcgcac
     7261 gcgccggccg tgactctcgt cgatgatgga gacgcgcttg agcaattccg ggtcgccggg
     7321 gaagcggtgg ttgacgtcgt gtaggaagcg atggttgatc tcgtaaatga tttccaggtg
     7381 gcgcggcagc acgcggccga acatttccac cggccaggtt tccaacgctt ccggcatcag
     7441 ggtatggttg gtgtaggcga agatgcgcgt cgtgaggttc catgcctgct cccacttgac
     7501 gcgctgcacg tccagcaact gatgcatcag ctccgccacg gcgatggccg gatgggtatc
     7561 gttcaactgg atggcgatct tctccggcag gctcttgagg tcatgcttgc cggcgaagaa
     7621 acggtgcagg atgtcctgga tcgatgcgga aacaaagaaa tactgctggc gcaggcgcag
     7681 ctcgcgcccc atcaccgagg tgtcgttggg gtacagcacc ttggacaggg tctcggtagt
     7741 gttcttctct tccaccgcac ccacgtaatc gccgacgttg aacgattcca ggttgaattc
     7801 gcgcgtggcc ttggccgacc acaggcgcag gttgttcacc gtctcggtct tgaagccggg
     7861 gatgggcacg tcgtaggcca tggccatcac ggtgtcggtt tccacccagt gatgttcgat
     7921 gcccccatgt ccgtcgggga attgcaccac gtggccgttg aaatgcaccg gatagaggcg
     7981 ttccggccgc tggaattccc acgggttgcc gtaacgcagc cagttgtccg ggtgctccac
     8041 ctgcttgccg tcctcgatgc gctggtggaa catgccgtac tcgtagcgga tgccgtagcc
     8101 cagacccggc aggtcgagcg tcgccatgga atcgaggaag caggccgcca gacggccaag
     8161 gccgccgttg ccaagcgcgg catcggtttc catctcctca acatgctcca tgtcctgccc
     8221 cagatccttc agggcggcgc gcaccggctg ctcgatatcc aggttgtgcg cagcgttgga
     8281 cagcgtgcgc ccgatcagga actccaggga gaggtaataa acccgcttgg gatcctgctt
     8341 gtcgtaggta cccaccgtat ccacccagcg ctcgatcaga tggtcgcgca cggtatgcgc
     8401 cacggtgtcg taccagtcgc gcgaggtggc gtagtgctcg gtcttgccgc cggtgaaaat
     8461 caggtgattg gccagcgatt tttcgatgga atgctcgtcg cagttgaggt agtgctggcg
     8521 tggggatttg ggtttgcggg gtttggtcat ggcatcagca gtcgttggtt gaacatgatt
     8581 ctgggtaagc acggattata ccagagcacc ccgccggact catggcctgc cggcagaaaa
     8641 aagaaacaac gatcagctaa tgctggtgct ggctacgcac ccgccaaccg gcaacgatat
     8701 gaaccgccag aaacagcagc agcaatacgc cgatgagcat gtgcgccaca ctggcgcaca
     8761 gcgccgcctg gtcttcaccg aggtagtaaa ttccgattgc ggtaatgcca agcagtagca
     8821 tcgaagccaa cagcgcgatg cctgtgcgat ggttcttgcg ctttttccag cccgcacgca
     8881 tgtgaatgct ccataatgcc ccaactacgg cggcagccag gaaggcggcg ctggcatgca
     8941 gcgccgccat ggcgatttgc ggatcgccgg tcagccgcca cggaacttcc cattccagtt
     9001 tcatttccag ggccgtcgga atcagcaggc tacccgagac gaacagggcg cccgtcgtcg
     9061 ccagcagtgt gcggtagaac caaccgggat agcccttcat gcgcatgctg ccactccatc
     9121 ggcatggctt acccatcggc cgccgagccg ctcgatcaag cctgcgcgct cgccatcgtc
     9181 ggcaaggcca gccaccttgg tcagcgcatc cgcccgccat gcgctatgcg ccatcacgct
     9241 ccagaccccg acctgagggg cgcgggattc ccctacgatc catgccggat acgcctggtc
     9301 atgcaccact ctgacgcacg aggtggcaat cgccgcatcc ataatcgcgc ccatgggccg
     9361 caagctgcca tccatctctc tgcgatggac cggcagcgcc aggtcgccaa atgcgcgcag
     9421 gtcaccgccg gcattgaccc agccggcccc ggcgccatgc cgttgcatcg cacgtatcgc
     9481 gcaatccacc gcatagcctt tggcgatccc gtccagcgtc acccgcaccg gccggcgcaa
     9541 ccttacttgt gtaccgcgta tctcgatgtc gccagccttt ccgcagtcga gcggcgcccg
     9601 cccgccgtga tcaggcaata cgccacgcct caccagggca ccacccaccg tgcagttgaa
     9661 taggccagcg ctggcttggg tcatggcgcg agccagccgc agcacccgga cgatctgcgg
     9721 atgcaaggcg acctcgccgc ctccggcctg attcaggcgg gataagtcac tgtgcggatc
     9781 atgaaagctc atcaggccct gcacctccgc cacagcatcg aaggcggcgt cgagcgcacg
     9841 ctccgccccc gctcctgact gaacgccaat ctcgacaaac gtacccagca agggccgcat
     9901 tcgtttcatg ctagtcactg gccagcgcca ccttctgcag cgccagcagc cgcttcacac
     9961 catccatgac gttgcggcag gacaaggtcg ccccgctgat gttaggcaca tcctcatcca
    10021 gtttgaacgg gtcggccagc gtcttgccca ccagattctt gcgccagccg gcttcgcgaa
    10081 tctgatcccc cttggtttcg cgataagtca tgacttcgat gccgaccaca tgcccgtcga
    10141 gcgacaggcc gaccccatag gtgatgaatt catgcttgcc aaccacttcg tcgacgatga
    10201 accaccccag ttgcttgcca tctttttcca cacgccagac cttttgctct tcccagcgct
    10261 ggcgcaggcc ggacagcgcc ttgatcttgt cgcgctgggc ttcacccagc ttgaccagca
    10321 tgggctgaaa tgcgtcagca tccggaaaca acgatttctg cgcctgtgca gcggtcagat
    10381 aatcctgtgc ataggctggc gcgacgatgg ccgccatggg cagcagccac aggccattca
    10441 tattagattt atgcatggtt ctgtactcag aagtcgatgc cgaatttgac gcggaattcc
    10501 tgctcggttt tttcgatcag ttgcagatca tgatcgttct ggcctggata ggtcagccca
    10561 ccacctctga tctgcgggaa ccaggtggcc gtcacccaga atttttcacc cccgtaatgc
    10621 aggttcggac cagcaaacaa ggaccaacgc tcctgcccca cctgggtctc gtactcggtt
    10681 tgatactgca gttcgccgcc gataaaccag ttgggcatga aacgataact caggcccgta
    10741 cccagagtca gttcaatttc catttccggg tctgtcggcc actcgtaatc ggggggcaga
    10801 ctggccatgc tgctc
//
LOCUS       DAOMFA010000045        10760 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_640_length_10760_cov_5.360579, whole genome shotgun sequence.
ACCESSION   DAOMFA010000045 DAOMFA010000000
VERSION     DAOMFA010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 10760)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10760)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10760
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_640_length_10760_cov_5.360579"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(120..1322)
                     /locus_tag="PKW44_03640"
     CDS             complement(120..1322)
                     /locus_tag="PKW44_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQR50710.1"
                     /translation="MLLADSSRYALQAITSHRLRSFLTLLGIAVGIAVVILLTSIGEG
                     VHRFVLGEFTQFGTNVVAIAPGKTKTGGAPPAGIPSTARLLTLEDAQSLRQLPSVTGV
                     TPIVWGNSEIGGNGRLRRTTVYGVDADFLRVFSAHVTVGSFLPREDADGARSFVVLGA
                     KLRNELFGTANPLGARVRIGGLHFRVIGVMAPKGQFLGIDLDDTAFIPAARAQEVYNR
                     EGLMEIDVTYRDGVPSASVAKAVTERLKIRHGSEDFTVTTQEDMLKTLSNILDILTAA
                     VGALGGISLLVGGVGIVTIMTIAVSERTNEIGLLVALGAPRRTILNLFLGESVVLAAI
                     GGALGLALGAGLAQLLRLVLPALPVHTPWNFAVGALLMSAIIGLLAGVLPAGRAARLD
                     AIEALRSE"
     gene            complement(1336..2262)
                     /locus_tag="PKW44_03645"
     CDS             complement(1336..2262)
                     /locus_tag="PKW44_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide-inducible genes activator"
                     /protein_id="HQR50711.1"
                     /translation="MTLTELRYIVAVARERHFGRAAQTCFVSQPTLSVGIKKIEEELG
                     VAIFERGSGEITLTPVGEQIVAQAARTLDEAAAIKNIARESGDPLAYPLRLGAIYTIG
                     PYLLPLILPIMRKLAPSMQLIIQESYTASLREQLKQGKLDVAILSLPFEEPGMVVQPL
                     YDEPFQVAMPANHPWQQREAIPPADLGQEPVLLLGAGNCFRDQVLQVCPNLSRSATAS
                     GMQQTLEGSSLETIRYMVASGVGMTILPCTATNASREENSMLRFRPFTLPSPKRRVAL
                     VYRKSFTRPQAVEAVRLSILQAALPCVEWIAQ"
     gene            2327..4618
                     /locus_tag="PKW44_03650"
     CDS             2327..4618
                     /locus_tag="PKW44_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020182262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HQR50712.1"
                     /translation="MFFQKSASSATARREPVPEKTAGLLREAWWLLLVVVGSYLATVL
                     TTYHRNDPGWSHSASDNAILHNAGGAFGAWLSDILLYLFGISAWWLVALAFYSMWLVY
                     LRLENRGSNERPVLFYNLSGFVMLLIASSALEAMHLRGLHAALPLAPGGMLGAVVQGA
                     LGKAFGHTGATMALLLAFATGFSLFTGWSWLTITEKIGAAIDVSFFFLRDRWRDWQDR
                     KAGAAAEQLREEYVESERKRTEDREPVQIEPPQLDIPRSSRVEKERQAPLFDALPDSP
                     LPPLHLLDEPKGQVEVQSAETLEFTSRLIERKLMDFNIEVKVVSALPGPVITRYEFEP
                     AAGVKGAQITNLAKDLARALSVVSIRVVETIPGKSCMGLEIPNPKRQIVHLSEILGSQ
                     VYADMSSPLAIAMGKDISGKPVVADLARMPHVLVAGTTGSGKSVAINAMILSLVYKAD
                     ASKVRLILVDPKMLELSVYEGIPHLLAPVVTDMRQAASALNWCVAEMDKRYRLMSALG
                     VRNLAGYNQKIREAEKEGGKLTNPFTLTPDNPEPLEEMPLIVVVIDELADLMMVVGKK
                     VEELIARLAQKARASGIHLVLATQRPSVDVITGLIKANIPTRVAFQVSSKVDSRTILD
                     HMGAEALLGQGDMLYQPPGTNYPQRVHGAFVSDSEVHRVVDYLKSMGAPDYVDGILTG
                     GEEVEAGDGELGGGGGETDPLYDEAVAIVLRTRRPSISAVQRHLRIGYNRAARLIEEM
                     ERAGLVSPMQSNGNREVLVPSGE"
     gene            4658..5266
                     /gene="lolA"
                     /locus_tag="PKW44_03655"
     CDS             4658..5266
                     /gene="lolA"
                     /locus_tag="PKW44_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019898029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HQR50713.1"
                     /translation="MLKKLLPLLLLLPGLVYADAIQQLKTFFQNTTTMRAQFHQTVLD
                     NMGGKVQDVTGSMQLSRPGKFRWDYNKPFVQLIVGDGHKVWLYDPELNQVTVRPLDKV
                     LGSSPAALLAGGTEIDRNFILKNDGGQGQLDWVEARPKDQDSGFERVLLGFKDGLLQE
                     MELHDNFGQTTVIEFQKVERNPRLDAQAFRFVPPQGADVVGE"
     gene            5266..6579
                     /locus_tag="PKW44_03660"
     CDS             5266..6579
                     /locus_tag="PKW44_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HQR50714.1"
                     /translation="MAERSLFAEKEPHAPLAERLRPKTLDEVVGQSHLLGEGKPLRLA
                     FTSGNLPSMILWGPPGVGKTTLARLIANTAEAEFIPLSAVLAGVKDIREAVERAELTL
                     QQSGHRTILFVDEVHRFNKSQQDAFLPFVESGLLTFIGATTENPSFEVNSALLSRAQV
                     FVLNPLSEVELGVLLERALELESGGLALQQEAREQILAYADGDARRLLNFLEGLFSAA
                     HAAGVNDIDLAFLDSALASKLRRFDKGGEAFYDQISALHKSVRGSNPDAALYWFLRML
                     DGGADPLYLGRRIVRMAIEDIGLADPRAQTMAVEACQIYERLGSPEGELALANAVIYM
                     AVAAKSNAAYMAYNQAKAFVAQDKSRPVPLHLRNAPTKLMKSLDYGKDYRYAHDEAEG
                     YAAGEQYFPDDMPPVKFYQPTPRGLEGKIAEKLAHLRELDRKARK"
     gene            6593..7888
                     /gene="serS"
                     /locus_tag="PKW44_03665"
     CDS             6593..7888
                     /gene="serS"
                     /locus_tag="PKW44_03665"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019922045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HQR50715.1"
                     /translation="MLDIQHFRTDLDGVAARLVARGYTLDTATFSALEGQRKEVQTRT
                     QELQAKRNATSKQIGIAKSRGEDVSAILAEVANLGDALKADEEKLAVIQAQMQDWLLN
                     VPNLPHASVSVGKSEADNVEVRRWGTPRGFDFEVKDHVDVGGALGLDFDTGAKLAGAR
                     FTFMRGQIARLHRALAQFMLDTQTAEHGYTECYTPYMVNADTLRGTGQLPKFEADLFA
                     AKKGGQEGEGEALYLIPTSEVTLTNTVRDEIVPLESLPIKLTAHTPCFRSEAGSYGRD
                     TRGMIRQHQFDKVEMVQIVHPDSSYDALEEMVGHAEAILQKLGLPYRVVSLCTGDMGF
                     GAAKTYDLEVWLPAQNTYREISSVSNCEAFQARRMQARFRNAAGKPELLHTLNGSGLA
                     VGRTLVAVLENYQNADGSVTVPGALRPYMGGVEKIAAAS"
     gene            7885..8682
                     /locus_tag="PKW44_03670"
     CDS             7885..8682
                     /locus_tag="PKW44_03670"
                     /inference="COORDINATES: protein motif:HMM:NF012730.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HQR50716.1"
                     /translation="MKLSQLLGINGVESHERLEARLWAKRLEWPILLVVLWIPIQWYL
                     EETAVITPLHGKIGDWLIWLVFLFETVLLTALVRDKLRYLRGNWMNPLIILGGLPLLW
                     HYTPLAGLLRSLRLVLALVLVLRLSKTLRVLLVRHRLGMTLAVSLVVVVVSGVAVTRL
                     DPSVGTVWDGMWWAWVTVATVGYGDIVPHNGAGRLFGALLILMGVVLVSLLTANLSAF
                     LIGGEVERVEREERREDVLLQEIAEKLERIEQRLDERERREPPASSS"
     gene            complement(8741..10465)
                     /gene="ybaL"
                     /locus_tag="PKW44_03675"
     CDS             complement(8741..10465)
                     /gene="ybaL"
                     /locus_tag="PKW44_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013966477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaL family putative K(+) efflux transporter"
                     /protein_id="HQR50717.1"
                     /translation="MPHDINLITTIAAGLGVALIFGFIASKLRMPPLVGYLLAGIAIG
                     PSTPGLVADVQLAGQLAEIGVMLLMFGVGLHFSLGDLLSVKRIAVPGALVQIMVATAL
                     GAATASLWGWGSGAALVFGLSLSCASTIVLLRALEARGVLDSINGKIAVGWLIVEDLV
                     MVLALVLLPTFATLLEAKTAGAPILTDANLWITLGITLTKVAGFIALMLVVGKRLFPA
                     LLWHLARTGSRELFTLCVIAVAVGVAYGSAMLFGVSFALGAFFAGMMLRESEFSHRAA
                     DELLPLRDAFSVLFFVSVGMLFEPRVLIDAPLQVLGVVAIIMFGKTLAAVALVLAFRY
                     PLSTALTVGASLAQIGEFSFILAGMGVALGLLSNEGQSLILAGALISIALNPLVFSAV
                     EPVRTLIFNKSTLARRLELSDDPLAALPMTVEEKFLSGQVVLVGYGRVGRRIGEALAT
                     RNIPFVVAEQNREIVEQLRAQDIPAVSGDAADPVVLVQAHIARAGMLVITTPNTFSAR
                     KMIETARTLNPAVEVVLRTDSDETMELLEKEQASKVFMAEHALAVSMTAHILARMGIG
                     YSGKNDGS"
     gene            complement(10531..>10760)
                     /locus_tag="PKW44_03680"
     CDS             complement(10531..>10760)
                     /locus_tag="PKW44_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lytic transglycosylase F"
                     /protein_id="HQR50718.1"
                     /translation="NPDSWADVKKMLPLLGREEYFSTLKYGFARGGAPVVFVESIRTY
                     YKILEKHEPQHTPIFPSFKLAKNSSRTTLVE"
BASE COUNT         2054 a         3291 c         3453 g         1962 t
ORIGIN      
        1 gataccgccg atctgtgccg ctgtaaaggc gaccacttct acctccggat cgtcgcggta
       61 ggccacgttg aagttgtgga agtcgcgccc ggcagccccc aagatgacaa tgcgctgttt
      121 cattccgacc gtaatgcctc gatggcgtcc agtctcgccg cacgccccgc tggcagcacc
      181 ccggccagta ggccgatgat ggcagacatc agcagtgcgc cgacggcgaa gttccacggc
      241 gtatgcaccg gcaaagcggg taacaccaag cgcagcaatt gcgccaaccc agcgccgagc
      301 gccaagccga gcgcgccgcc gatggccgcc agcaccaccg attcgcccag gaacaagttg
      361 aggatagtgc ggcgtggcgc gcccaaggcc accagcaggc cgatctcgtt ggtacgctcg
      421 gaaaccgcaa tggtcatgat ggtgacgatg ccgactccgc ccaccagcag cgaaattcca
      481 cccagtgcac ccaccgcggc ggtgaggata tcaaggatat tggacagcgt tttcagcata
      541 tcctcctgcg tggtgacggt aaaatcctcg ctgccgtggc gtattttcag gcgttccgtc
      601 accgccttgg ccacactggc cgagggcacg ccatcgcggt aggtgacgtc gatttccatt
      661 aggccttcgc ggttgtagac ctcctgtgcc cgcgccgccg ggatgaacgc cgtatcgtcg
      721 aggtcgatac cgaggaactg gcctttaggt gccatcaccc cgatcacgcg gaaatgcagc
      781 ccaccgatgc gcaccctggc cccgagcggg ttggcggtgc cgaacagttc gttgcgcagc
      841 ttggccccca gcaccacgaa actgcgtgcg ccgtcggcat cctcgcgcgg cagaaaactg
      901 cctaccgtca cgtgcgcgct gaacacgcgc aggaaatccg catctacgcc atatactgtc
      961 gttcgtcgca gccgcccgtt gcctcctatc tcggagtttc cccagacgat gggcgtcacc
     1021 ccggtgacgc tgggcagctg ccgcagcgac tgtgcatcct ccagcgtcag cagccgcgcc
     1081 gtgctgggaa tgccggcagg cggcgctcca ccggtcttgg tcttgccggg agcaatggcc
     1141 accacgttgg tgccgaactg ggtgaattcg cccagcacga agcggtgtac gccctcgccg
     1201 atggaagtga gcagaatcac caccgcgatg ccgaccgcga taccgagcag cgtcaggaag
     1261 ctgcgcagcc gatgcgaggt gatggcttgc agggcgtagc gggaagaatc ggcgagaagc
     1321 atggctatcc ggccgctact gtgcaatcca ctccacgcaa ggcaaagcgg cttgcaggat
     1381 ggacaggcgt actgcttcaa ccgcctgcgg gcgggtaaaa ctttttcgat agaccagcgc
     1441 cacgcggcgc ttggggctag gcagggtaaa ggggcggaag cgcagcatgc tgttctcctc
     1501 gcgcgaggca ttggtcgcag tacagggcag aatggtcatg ccgacgccgg aggcgaccat
     1561 gtagcgtatg gtttccaaag aactaccttc cagcgtctgc tgcatgccgc tggcggtcgc
     1621 gctcctgctc aggttggggc agacctgcag tacctggtcg cggaagcagt tgccggcacc
     1681 cagcaacagc accggctcct gtcccaagtc cgctggcggg atggcctcgc gctgctgcca
     1741 cgggtggttg gcaggcatgg cgacctgaaa cggctcgtcg tagagcggct gcaccaccat
     1801 gcccggctcc tcgaaaggca gcgacaggat ggcgacatcc agcttaccct gtttgagctg
     1861 ctcgcgcagg ctggcagtgt agctttcctg aatgatgagc tgcatggagg gcgccagttt
     1921 gcgcatgatc ggcaggatca gcggcagcag gtaggggccg atggtgtaga tggcgccgag
     1981 acgcagcgga taggccagcg gatcgccgct ttcccgggcg atattcttga tggctgccgc
     2041 ctcgtccagg gtgcgcgcgg cctgcgcgac gatttgctcg cccaccggcg tcagcgtgat
     2101 ttcaccagag ccgcgctcga agatggcgac gcccagttcc tcctcgattt tcttgatgcc
     2161 gacgctcaag gtcggctggc tgacgaagca ggtctgtgcg gcgcgcccga agtgccgttc
     2221 gcgggcgacg gcgacgatat aacgcaattc ggtcagggtc atggttcgta atcagtaaag
     2281 tccggtaaaa tccgatttta cgcggatatc gttggtactc tacagcatgt ttttccaaaa
     2341 aagcgcttcg tccgccacag ccagacgcga acctgttccg gaaaaaacgg cgggcctgct
     2401 gcgcgaggcg tggtggctgc tactggtggt ggtcggcagc tatctggcca cggtgctcac
     2461 tacttatcat cgcaatgatc cgggctggtc gcacagcgct agcgacaatg ccatcctcca
     2521 taatgccggc ggtgcttttg gcgcctggct gtcggacatc ctgctttacc tgttcggtat
     2581 ttccgcctgg tggctggtgg cactggcgtt ttattccatg tggctggttt atctgcgact
     2641 ggaaaacagg ggctcgaacg agcggccagt gctgttttac aacctgtctg gcttcgtcat
     2701 gctgctgatc gcttccagtg cgctggaggc catgcatctg cgcggcctcc atgccgcctt
     2761 gccactggcg ccgggaggca tgctgggagc ggtggtgcag ggcgccttgg gcaaggcgtt
     2821 cggccatacc ggcgccacca tggctctgct gctggcgttc gccaccggct tcagcctgtt
     2881 caccggctgg tcgtggctga ctattacgga aaagatcggc gccgccatcg acgtcagctt
     2941 tttcttcctg cgcgaccgct ggcgcgactg gcaggatcgc aaggcgggcg cggcggctga
     3001 acagctgcgc gaagagtacg tggagagtga acgcaagcgc accgaagacc gcgagccggt
     3061 gcagatcgag ccgccgcaac tggacatccc caggagcagc cgtgtcgaga aggagcgcca
     3121 ggcgccctta tttgacgctt tgccggattc gccgctgccg ccgctgcacc tgctggacga
     3181 gcccaagggg caggtggaag tacagagtgc ggagacgctg gaattcacct cgcgcctgat
     3241 cgagcgcaag ctgatggatt tcaacatcga ggtcaaggtg gtgtctgcct tgccggggcc
     3301 ggtgattacc cgctatgagt tcgagccggc agcgggcgtc aagggtgccc agatcaccaa
     3361 cctggccaag gacctggcgc gggcgctgtc ggtggtcagc atccgcgtgg tggaaaccat
     3421 cccgggcaag agctgcatgg ggctggaaat ccccaatccc aagcgccaga tcgtgcacct
     3481 gtctgaaatc ctcggctcgc aggtttacgc cgacatgagt tcgccgctgg ccatcgctat
     3541 gggcaaggac atctccggca agccggtggt cgccgatctt gcccgcatgc cgcacgtgct
     3601 ggtggcgggc accaccggct ccggcaaatc ggtggcgatc aatgccatga ttctgtcgct
     3661 cgtctacaag gcggatgcca gcaaggtacg gctgatcctg gtcgatccca agatgctgga
     3721 actgtcggtg tatgaaggca tcccgcacct gctggcgccg gtggtgaccg acatgcgcca
     3781 ggctgccagc gcgcttaact ggtgcgtggc cgagatggac aagcgctatc gcctgatgtc
     3841 ggcactgggg gtgcgcaatc tggcgggcta caaccagaaa atccgcgagg cggaaaagga
     3901 aggcggcaag ctcaccaatc cgttcacgct gacgccggac aaccccgagc cgctggagga
     3961 aatgccgctg atcgtggtgg tgatcgacga actggccgac ctgatgatgg tggtgggcaa
     4021 gaaggtggaa gaactgatcg cccggctcgc tcaaaaagcg cgcgcctccg gcatccacct
     4081 ggtgctggcg acacagcgcc ccagcgtgga tgtcatcacc ggcctgatca aggccaacat
     4141 tcccacccgc gttgccttcc aggtgtccag caaggtggat tcgcgcacca tcctcgacca
     4201 catgggtgcc gaggccctgc tggggcaggg cgacatgctg tatcagccgc ccggcaccaa
     4261 ttacccgcag cgggtgcatg gcgcttttgt gtcggatagc gaagtgcacc gcgtggtgga
     4321 ttacctcaaa tccatgggcg cacccgacta cgtcgacggc atcctcaccg gcggcgagga
     4381 agtcgaagcc ggtgatggcg agctgggcgg aggtggcggc gagaccgacc cgctctacga
     4441 cgaagcggtc gccatcgtgc tcagaacgcg ccgcccttcc atttctgccg tgcagcgcca
     4501 tttgcgcatc ggctataacc gtgccgcgcg cctgatcgag gagatggagc gcgccggtct
     4561 ggtttcgccc atgcagtcca acggcaaccg cgaggtgctg gtgccgtcgg gcgaatgacc
     4621 taccccaggg cgccaggatt cattacaagg atatttcatg ctcaagaagt tgttgccatt
     4681 gctattgctg ctgccaggcc tggtatatgc cgatgccatc cagcagttga agacgttttt
     4741 ccagaacacc accaccatgc gcgcccagtt ccaccagacc gtgctcgaca acatgggggg
     4801 caaggtacag gacgtgaccg gtagcatgca gctgtcgcgc cccggcaaat tccgctggga
     4861 ttacaacaag ccgtttgtgc aactgatcgt tggtgacggc cacaaggtct ggctgtacga
     4921 cccggaactc aaccaggtca cggtgcgccc gctggacaag gtgctgggct ccagcccggc
     4981 ggcgctgctg gcgggcggaa ccgaaataga caggaacttc atcctcaaga acgatggggg
     5041 tcagggccag ctggattggg tcgaggccag gccgaaggac caggacagcg ggttcgagcg
     5101 cgtgctgctc ggattcaagg atggtctact gcaggaaatg gaactgcacg acaattttgg
     5161 ccagaccacg gtgatcgaat tccagaaggt ggagcgcaac ccgcggctgg acgcacaggc
     5221 gttccgcttc gtgccgccgc aaggggcgga tgtggtgggg gagtagtggc cgagcgcagc
     5281 ctgtttgccg aaaaggagcc gcacgctccg ctggctgagc gcctgcgccc gaaaaccctg
     5341 gatgaagtgg ttgggcaatc ccatctgctg ggcgaaggaa agccactgcg tctggcgttc
     5401 acctccggca atttgccgtc gatgatattg tggggcccgc ctggcgtggg caagaccacg
     5461 ctggcccggc tcatcgccaa cacagccgag gccgagttca ttccgctctc cgccgtgctg
     5521 gccggcgtca aggatatccg cgaggcggtg gagcgcgccg aactgacgtt gcagcagtct
     5581 ggccaccgca ccatcctgtt tgtggatgaa gtgcaccgct tcaacaagtc gcagcaggac
     5641 gccttcctgc cgttcgtaga aagcgggctg ctcactttca tcggcgcgac gaccgaaaat
     5701 ccctcattcg aagtgaacag cgcgctcttg tcgcgggcgc aggtgttcgt gctcaatcca
     5761 ttgtccgagg tggaactggg cgtgcttctg gaacgcgcac tggaactgga atccggcggg
     5821 ctggccttgc agcaggaggc gcgcgagcaa attctggcct atgccgacgg cgatgcgcga
     5881 cggctgctga atttccttga aggtttgttc agcgccgccc atgctgccgg cgtcaacgac
     5941 attgatcttg ctttcctcga cagcgctctg gcctccaagc tgcgccgctt cgacaagggc
     6001 ggcgaggcgt tttacgatca gatttccgcg ctgcacaagt cggtgcgcgg ttccaacccg
     6061 gatgccgcgc tgtactggtt tctgcgcatg ctggacggcg gcgccgaccc gctttacctg
     6121 gggcgccgta tcgtgcgcat ggccatcgag gacatcggcc tggccgaccc ccgcgcccag
     6181 accatggcgg tcgaagcctg ccagatttac gaacgcctcg gctcgcctga aggcgagctg
     6241 gcgctggcca acgcggtgat ctatatggcc gtggccgcca agagcaacgc cgcctacatg
     6301 gcctacaacc aggccaaggc cttcgtcgcg caggacaagt cgcggcctgt tccattacac
     6361 ctccgcaacg cgcccaccaa gctcatgaag tcgctcgact acggcaagga ctaccgttac
     6421 gcccacgacg aggcggaagg ctatgccgcc ggcgagcagt attttccgga cgacatgccg
     6481 ccggtgaagt tttatcaacc cacgccgcgc ggactggaag gtaaaatagc cgaaaagctg
     6541 gcgcacctgc gcgagttgga cagaaaagcc agaaaataaa ccggagtttc aattgctcga
     6601 catacaacac ttcagaaccg acctcgatgg cgttgccgca cggcttgtcg cccgtggtta
     6661 taccctcgac accgccactt tctccgccct ggaagggcag cgcaaggaag tgcagacccg
     6721 tacccaggag ctgcaggcca agcgcaatgc gacgtccaag cagatcggca tcgccaaatc
     6781 cagaggcgag gatgtgtcgg cgatcctggc cgaagtcgcc aaccttggcg acgcgctgaa
     6841 ggccgacgag gaaaagctgg cggtgatcca ggcgcagatg caggactggc tgctgaatgt
     6901 ccccaacctg ccgcatgcgt cggtgtcggt aggcaagtcc gaggcggata acgtggaagt
     6961 gcgccgctgg ggtacgccac gtgggttcga tttcgaagtg aaagaccatg tggacgtggg
     7021 tggcgcgctg ggactggatt tcgataccgg cgccaaactt gcaggggcgc gcttcacctt
     7081 catgcgcggg cagattgcac ggctgcacag ggcccttgct cagttcatgc tggatacgca
     7141 gactgcagag catggctata ccgaatgcta tacgccgtat atggtgaacg ccgataccct
     7201 gcgcggcacg gggcagctgc ccaagttcga ggctgatctg ttcgccgcca agaagggcgg
     7261 gcaggaaggg gagggtgaag cgctttatct gatcccgact tccgaagtca ccctcaccaa
     7321 caccgtgcgc gacgagatcg tgcctctgga aagcctgccg atcaagctca ccgcccacac
     7381 cccgtgcttc cgttccgagg ccggctctta cggccgcgac acgcgcggca tgatccgcca
     7441 gcaccagttc gacaaggtgg aaatggtgca gatcgtacat ccggactcaa gctatgacgc
     7501 gctggaagaa atggtcggcc atgccgaggc tatcctgcag aaactggggt tgccgtatcg
     7561 ggtggtgtcg ctgtgcaccg gcgacatggg gttcggcgcg gccaaaacct acgacctcga
     7621 ggtgtggctg ccggcgcaga acacctaccg cgaaatctcc tcggtttcca actgcgaggc
     7681 attccaggcg cggcgcatgc aggcgcgctt ccgcaacgcc gccggcaagc cggaactgct
     7741 gcacaccctg aacggctccg gcctggctgt ggggcgcacg ctggtggcgg tgttggaaaa
     7801 ctaccagaac gccgatggca gcgtcaccgt tcccggggcg ctgcggccat acatgggcgg
     7861 cgtggaaaag atcgccgcgg cttcatgaaa ctttctcagc tgctcggcat taacggggtc
     7921 gaatcgcatg agcgtctgga ggcgcgcctt tgggccaagc gcctggagtg gccgatattg
     7981 ctggtggtgt tgtggatccc gatacagtgg tatctggagg aaacggcagt cattactccg
     8041 ctgcatggca agatcggcga ctggctgatc tggctggtgt tcctgttcga gaccgtcctg
     8101 ctgacagcct tggtgcgcga caagctgcgt tatctgcgtg gcaactggat gaacccgctg
     8161 attatcctgg gcggcttgcc cctcctatgg cattacacac cgctggccgg cctgttgcgc
     8221 agcctgcggc tggtgctggc gctggtgttg gtgctgcgcc tgtcgaagac gctgcgtgtc
     8281 ctgctggtgc ggcatcggct tggcatgacg ctggccgttt cgctggtggt ggtggtggtg
     8341 tccggcgtcg cggtgacgcg cctcgatccc tccgtgggca cggtgtggga tggtatgtgg
     8401 tgggcctggg tgacggtggc gacggtgggt tacggcgaca tcgtgccgca caacggcgca
     8461 gggcgattgt tcggcgcctt gctgatcctg atgggtgtgg tgctggtgtc gctgctgacc
     8521 gccaatcttt ccgccttcct cattggcggc gaagtggaac gggtcgagcg tgaagagcgg
     8581 cgcgaggatg tgctgctgca ggagatcgcg gagaaactgg aacgcatcga gcagcggctg
     8641 gacgagcggg aaagacgcga accgccggct tcctcatctt gaaccgaggg ctacgggatg
     8701 tgccgtctcc ggtgctgatg cacgggcggg ttgaacgcct tcaggaaccg tcgttcttgc
     8761 ctgagtagcc gattcccatg cgggctagga tatgcgctgt catgctgacg gccagcgcgt
     8821 gttcagccat gaacaccttg ctggcctgtt ccttctccag cagctccatt gtttcatcgc
     8881 tgtcggtgcg cagcaccact tccactgccg gattgagggt gcgcgctgtt tcgatcatct
     8941 tgcgggcgct gaaggtgtta ggcgtcgtga tgactagcat gccggcgcgc gcgatatggg
     9001 cctgtaccag caccacgggg tcggcggcat ccccggagac tgccggaata tcctgtgcgc
     9061 gcagttgttc gacgatttcc cggttctgct ctgccactac gaacgggatg ttgcgcgtgg
     9121 ccagcgcctc gccgatgcgg cgccccacgc gaccatagcc caccaacacc acctggccac
     9181 tgagaaattt ttcctcgact gtcatcggta atgccgccag cggatcgtcg ctcagttcca
     9241 gacgccttgc cagcgtggat ttgttgaaaa tcaaggttcg taccggttct accgccgaga
     9301 acaccaaggg attaagcgca atcgaaatca aggcgccggc cagaatcagg ctttgccctt
     9361 cgttggagag cagacccagc gcgaccccca tgcctgccag gatgaaggag aactccccga
     9421 tctgcgccaa actggcacct actgtcagcg cggtactcaa cgggtagcgg aaggccagca
     9481 ccagcgcgac ggctgccagt gttttgccga acatgatgat ggccacgact cccagcactt
     9541 gcagcggcgc atcgatcagc acgcgcggtt cgaacagcat gcccacggac acgaaaaaca
     9601 gtaccgagaa tgcatcgcgc aacggcagaa gttcgtcggc ggcgcgatgg ctgaattccg
     9661 attcgcgcaa catcatgccg gcaaagaacg cccccagcgc gaacgatacg ccgaacagca
     9721 tggctgaacc gtaggccacg ccgaccgcca cggcgatgac gcacagggtg aacaactcgc
     9781 gcgacccggt gcgtgccaga tgccacagca gggccggaaa caggcgcttc ccgaccacca
     9841 gcatcagggc gatgaagcca gcgactttgg tcagagtgat gcccagcgtg atccaaagat
     9901 tggcgtcggt caggattggc gcccccgcgg tttttgcctc cagcagggtg gcgaatgtgg
     9961 gcagcagcac cagcgccagc accatgacca aatcttcaac gatcagccaa cccacggcga
    10021 tcttgccgtt gatggaatcc agtaccccgc gcgcctccag cgcgcgcaac agcacgatgg
    10081 tgctggcgca ggacagggac aagccgaaca ccagcgcggc gccggaaccc cagccccaca
    10141 gcgaggcggt agcggcgccc agggcagtcg ccaccatgat ttgcaccagc gcgccgggaa
    10201 ccgcgatgcg cttgaccgac agcaggtcgc ccagtgagaa atgcagccct acaccgaaca
    10261 tcagcagcat cacgccgatt tctgccagtt gtccggccag ttgtacgtcg gcaaccaagc
    10321 ctggagtcga cgggccaatc gcgataccag ccagcagata gccgaccagc ggcggcatgc
    10381 gcagtttgga agcgatgaaa ccgaagatga gcgcgacgcc caagccggcg gcgatggtgg
    10441 tgatcaggtt gatgtcatgc ggcacgggct agcgttcctt ggagacgatt gcttctggtg
    10501 gcggatgtca tccgtgcgac aggcagggcg ctattctacc aaggtggtgc ggctgctgtt
    10561 tttcgccagc ttgaagctgg ggaatatcgg cgtatgttgg ggctcgtgtt tctccagtat
    10621 cttgtaataa gtgcggatgg attccacgaa gaccaccggc gccccgccgc gggcaaagcc
    10681 atatttcagc gtcgaaaaat attcctcgcg tcccagcagc ggcagcattt tcttgacgtc
    10741 ggcccaggaa tccgggttca
//
LOCUS       DAOMFA010000046        10663 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_652_length_10663_cov_5.100490, whole genome shotgun sequence.
ACCESSION   DAOMFA010000046 DAOMFA010000000
VERSION     DAOMFA010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 10663)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10663)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10663
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_652_length_10663_cov_5.100490"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2231
                     /locus_tag="PKW44_03685"
     CDS             <1..2231
                     /locus_tag="PKW44_03685"
                     /inference="COORDINATES: protein motif:HMM:TIGR03623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HQR50719.1"
                     /translation="RMLELQIELVKHAELPETICLAGFDRISPQEQRLFDALTMRGAT
                     LRPWVAGLESPGHAVKKAFDEAEAECCAAAAWVCQRLTDDPSSRLAIVVPELANLRPT
                     LQSMLDDALHPESMHPAQAEIPRAYDFSLGQPLAEQPLVATAVALLRLAFGRQATQQE
                     CSTLLRDVYWSAGRGEADARARLDATMRRKLPATISFDQLLRHARKAQEKGLGIGRLV
                     QHLEGLQQKNVSTRQRASAWARTFAQALEAAHWPGERGLSSHEFQARQAWKETLQVFS
                     GLDPLLDKLSAGDALRQLSRLLRERIFQPESAHVPQIQVMGMLEATGQPLDALWVMGM
                     NDHTWPPPARPNPLLPAEVQRSAGTPNSCSRVQAEFALAIHQRLLRTAPELVFSWAHR
                     DGERELRVSPLLKDIPLAEQGFELAKTLAEQLAVPAEMQWLQDHQAPRVEEDEKVRGG
                     AGLFQAQAVCPAWGYYQYRLGARALEEPVDGLDSMARGSLLHAVLQAFWSGRDSEYLN
                     NMDQAAQQAAVQQAVEAGVKQFSQDLDESLPTHFLALEKLRLAALLDGWLALERERLP
                     FTVQDCERNVKLDIKGISVEFKLDRVDVLPDGRMVVIDYKTSAQLSPNSWAEDRISEP
                     QLPIYAALVLIEGEVAAVCFAKVRAGEHQFVGIASDAGTLPGVKGLEEARKVFDAEKF
                     PEWHALLQHWRDSLEAIADEIRAGEAAVRFEDEEQLAWCEVKPLLRLPERKLQLEREG
                     KR"
     gene            2228..5545
                     /locus_tag="PKW44_03690"
     CDS             2228..5545
                     /locus_tag="PKW44_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HQR50720.1"
                     /translation="MTAGPNLLQQDAANRERALALESFIVEAPAGAGKTELLTQRYLK
                     LLAVVQEPEEIVAITFTNKAAAEMRSRILQSLQDVADGVAIDKPHKQKTRELALKALA
                     RSAEKQWNLLEQPARLRINTIDSLCSFLARQMPLMSRFGGQPAVVDDAFIHCEAAARR
                     ALATLEDEAGDGVVTEALRYLDNDAVRLTDLLARMLAKRDQWLHHTTHVQEDVEMALR
                     QLIQQDIAQAASLLSPDLQQRLMPVARFAASNLECNHLISLLLDWDSTILARPEALPL
                     WRGVCELLLTSGDDFRKALDKRQGFPATDDGRKQKEALLEIIAMLPSPQPLARIRCLP
                     DAQSEEEKRIVAALARLLQLAAAHLLIVFQETGEVDFVEVAGRALAALQDDDGVTDLA
                     LKLDYKIQHLLVDEFQDTSPMQVELLRRMTQGWLPDDGRTLFCVGDPMQSIYRFRKAD
                     VGLFLQVANYGIGDLQLQRLHLTRNNRSCPAVVDWVNSAFEKMFPKQDDEERGAIRYR
                     PFAATKASEPNEGVLVHSLVLGADSRGDDMAAEEARFLTALIARERALDPKASIAVLV
                     RARAHLEALVVEIRSRHPDLRFQAVEIEQLGGRQTVQDIHALTCALFHRADRVHWLAI
                     LRAPWCGLTLADLHMLAADDHYANIWQLMQDEARLSRLSDDGRKRLLHVRDVIAEAYA
                     HQGRQPMRRWVESVWLRLGGAACLWDAGDVRDVQAFLDLLERMEVFDAAQLQSRMEKL
                     YAAPDVQADESLQFMTIHKSKGLEFDTVILPGLHRTSKNGDPQLLLWEEVAIEGTPSQ
                     LVAAPYVPRHKRDDLPSTYDYLQNLERERESNETTRVLYVAATRTRRRLHLVGAVRPD
                     GKGEIKAPANSLLRLLWESVGGEFLNAEPRAEIVATETSSAFIPSLVRLPQVAVPELL
                     QSGTPAAVRPAFDEAATDTNIRSIDTSCGTLVHLYLEMMADDGLQDWSSQRLHGLQFA
                     MQTWLQGHGHARDEARQGAERVLHALCHTLDSEAGQWVLASRGSAASELALATADGSQ
                     FATYVIDRTFVEDGVRWVIDYKSARLDTVEEDILTREAARYRPQLARYAALFREEGLP
                     IRMAVFFVAYGKLVELD"
     gene            complement(5647..6453)
                     /locus_tag="PKW44_03695"
     CDS             complement(5647..6453)
                     /locus_tag="PKW44_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018284971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HQR50721.1"
                     /translation="MNIQEILQQVQTTAIHVGLQIVAAIVFWVVGRWIIGLIGEIVRK
                     GLERQKVDPTLLRYIGSIVAVTLNIALALAILSYFGVETTSFAALIAGIGIAIGAAWG
                     GLLSNFAAGAFLIVLRPFKVGDFVTAGGITGTIKEIGLFATAINTPDNVLTTVGNSRI
                     FADTIQNFTANPYRRVDIKAQLNGSVDHRDAIARLKTRIDGIANVLQDPAPNLELLEF
                     TAAGPVLAVRPYCHNDHYWQVYFDTNVAIRDAFGEAGYPAPAQPIEVKQK"
     gene            complement(6559..7185)
                     /locus_tag="PKW44_03700"
     CDS             complement(6559..7185)
                     /locus_tag="PKW44_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HQR50722.1"
                     /translation="MLEKLIAILAGWIIAVISTLGYGGIVLLMGIESACIPLPSEIIM
                     PFSGYLVFKGEMALWGVALAGAVGCVLGSIPAYYLGMYGGRPLVEKYGKWVLISHHDL
                     HIADRWFEKHGEIIIFIGRLLPAVRTFIAFPAGIARMHMGRFIAYTFAGSLIWCWVLA
                     WLGMKLGENWTGLKVYFHEFHYVIAAAAVIFVVWYVRRHIRAVRGHHH"
     gene            complement(7186..10596)
                     /gene="mfd"
                     /locus_tag="PKW44_03705"
     CDS             complement(7186..10596)
                     /gene="mfd"
                     /locus_tag="PKW44_03705"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HQR50723.1"
                     /translation="MQAHLPIPAPGKTERVSDLRRGADSLELARLARTLKKPLVILSA
                     SAFDAQRLLEEMRWFEPQLAIHLLPDWETLPYDHFSPHPDLVSERLATLYQITQNACD
                     VIIIPVGTALVRLPPQAFLAAHTFMLKKGQKLDLEALRAQCAEAGYHHVNQVMSPGEF
                     SVRGGLVDLFPMGSALPYRLDLFDDEIETVRTFDVDTQRSLYPVAEIRLLPAREFPLD
                     DKGIAKFRQSFRETFEGDPSKCRIYKDISNGIAPGGIEWYLPLFFEQTATLFDYLPKD
                     AVLCLHGELDQAAQTFWQETQTRHRLLDHDRERPILPPQSLLVPVDAFFAATHGFGRL
                     QLSLEEGAPSLPDLNIEHRSELPLHRLQGFLQDFDGRALIAAESLGRRETMMQLLASH
                     GLRPKPCEDWAQFSASGEPLMLVVAPLHNGFRSDDIAFITEAELYPSQVRQERRREKD
                     RARSTEGMLKDLSELRENDPVVHEQHGVGRYRGLINLDFGEGETEFLLLEYAPPAKDS
                     PPDKLYVPVSQLFLISRYSGGPPESAPLHRLGSGQWDKARKRALKQIRDTAAELLNLY
                     AMRAARKGHAFKLGLHDYEAFAENFPFEETPDQLAAIEAVINDMQSGRPMDRLVCGDV
                     GFGKTEVALRAAFVAVMGGRQVAVLVPTTLLAEQHYNNFTDRFADWPVKIAEISRFRT
                     AKEQTEALKGLAEGSIDIIIGTHRLIQKDVHFKNLGLVILDEEHRFGVRQKEQLKAMR
                     AEVDVLTLTATPIPRTLSMAMEGLREFSVIATPPQKRLSIKTFVSPWSEGVIREAVMR
                     ELKRGGQVYFLHNEVESIYAMRDRLEKMLPEARIGIAHGQLRERELEHVMRDFYHQRF
                     NLLLCSTIIETGIDVPTANTIIMNKADRFGLAQLHQLRGRVGRSHHQAYAYLLTEPDV
                     KVTPQAQKRLDAIQLMEDLGAGFHLAMHDLEIRGAGELLGDSQSGEMQEIGFNLYSEM
                     LAHAVKQLQAGKEPNLDAPLGVTTEINLHTPALLPDAYCPDVHERLVIYKRLANCTTG
                     DQLQAMQEELIDRFGLPPPQTEALLECHKLRINAKPMGIVKIDASDAAIQLQFDLQAD
                     LDPMKLVRLLQTNRHARMNGPDKLRVTIAKENILQRAEFIKTLLKDLA"
BASE COUNT         1976 a         3127 c         3576 g         1984 t
ORIGIN      
        1 cgcgcatgct ggaactgcaa atcgaactgg taaaacatgc tgagttgccg gaaaccattt
       61 gcctcgccgg tttcgatcgt atcagtccgc aggagcagcg gttgttcgat gcgctaacca
      121 tgcgcggtgc caccttgcgt ccttgggtgg ctgggctgga aagcccgggg catgcggtca
      181 aaaaagcgtt tgacgaggct gaagccgaat gttgtgcggc ggcggcctgg gtgtgccaac
      241 gcctgaccga tgacccgtcc tcccggctgg ccatcgtggt gccggaactg gctaatttgc
      301 gccccacctt gcagtctatg ctggatgacg cactgcaccc ggaaagcatg catccggcac
      361 aggctgaaat cccgcgagcg tacgattttt cccttggcca gccgctggcg gaacaaccgc
      421 tggtggcaac cgccgtggcc ttgcttcggc tggcattcgg ccgtcaggct acgcagcagg
      481 aatgtagtac gctgctgcgt gatgtctact ggtcggcggg gcgcggcgag gctgatgcca
      541 gagccagact ggacgcaacg atgcggcgca aactgcccgc caccatctct tttgaccagc
      601 tgctgcgaca tgccagaaag gcgcaggaga aaggcttggg aataggcagg ttggtgcagc
      661 atctcgaagg gctgcaacaa aaaaacgtct ccaccaggca gcgagcgtcc gcctgggcgc
      721 gcacttttgc ccaagcgctg gaagctgccc actggcctgg cgagcgcggt ttatccagcc
      781 acgagtttca agcgcgccag gcatggaagg aaacactgca ggttttttcc gggcttgatc
      841 cattgctgga caagcttagc gccggcgatg cgctgcgcca gctatcccgc ctgctgcgtg
      901 agcgtatctt tcagccggaa tccgcccatg tgccgcagat ccaggtcatg ggaatgttgg
      961 aggcaactgg ccagccgctg gatgccttgt gggtgatggg catgaacgac cacacctggc
     1021 cgccgcccgc ccgcccgaat ccgctgctgc cggccgaagt gcagcgcagc gccggcacgc
     1081 ccaattcctg cagccgggtg caggccgagt ttgcgctagc catccatcag cggttgctgc
     1141 gcaccgcgcc ggaactggtg ttctcctggg cgcatcgcga tggcgagcgt gaactccgcg
     1201 tcagcccgct gctgaaagac atcccgctgg ccgaacaagg gtttgagctt gcgaaaacgc
     1261 tggccgagca gctcgccgtg cctgccgaga tgcagtggct gcaggatcat caggcgccca
     1321 gggtggaaga ggatgagaag gtacgcggcg gggcggggct gttccaggcc caggctgtct
     1381 gtccggcctg gggctattac caataccggc tgggagcgcg tgcgctggag gaaccagtgg
     1441 acgggctgga cagcatggcg cgcggtagct tgttgcacgc cgtgctgcag gcgttctgga
     1501 gcgggcgcga ttctgaatac ctgaacaaca tggaccaagc tgcgcagcaa gccgcggtgc
     1561 aacaggcggt ggaagccggc gtgaagcagt tctcgcagga tctggatgaa agcctgccta
     1621 cgcacttcct cgcgctggaa aaactgcggc tggccgcgct gctcgacggc tggctggcgc
     1681 tggaacgcga gcgcctcccc ttcaccgtgc aggattgcga acgcaacgtg aagctggaca
     1741 tcaagggcat cagcgtggag ttcaagctgg accgcgtgga tgtattgccg gacgggcgca
     1801 tggtggtgat cgactacaaa accagcgcgc agctcagccc caacagttgg gcggaagacc
     1861 gcatcagcga gccgcagctc cccatctacg ccgcgctggt gctgatcgag ggcgaggtgg
     1921 cggcggtgtg tttcgccaag gtgcgcgccg gcgaacacca gttcgtcggt atcgccagtg
     1981 atgccggaac tttacccggc gtgaagggct tggaagaagc gcgcaaggtg ttcgacgcgg
     2041 agaaattccc ggaatggcac gccttgctgc aacactggcg cgacagcctg gaggccattg
     2101 ccgacgaaat cagggcaggc gaagccgccg tacgctttga ggacgaagag caactggcct
     2161 ggtgtgaagt gaagccgctg ctacggctgc cggaaaggaa attgcagctg gagcgggagg
     2221 gcaagcggtg acggccggtc cgaatctgct gcagcaggat gccgccaacc gcgaacgggc
     2281 gctggcgctg gagtccttca tcgtcgaagc cccggccggc gcgggcaaga ccgagttgct
     2341 cacgcagcgc tatctgaagc tgctggcggt ggtgcaggaa ccggaggaga ttgtcgccat
     2401 caccttcacc aacaaggcgg cggcagagat gcgtagccgt atattgcaga gtttgcagga
     2461 cgtagccgac ggcgtggcta tcgacaagcc gcacaagcag aagacacgcg aactggcgct
     2521 caaggcgctg gcgcgttcgg cggaaaaaca gtggaacctg ctggaacaac cggcgcgcct
     2581 gcggatcaac accattgact ccctgtgcag cttcctggcc cggcaaatgc cgctgatgag
     2641 ccgcttcggc ggccagcccg cagtggtgga cgatgctttc atccattgtg aggcggcggc
     2701 gcggcgtgcg ctggccacgc tcgaagatga ggcgggcgat ggggtggtga ccgaggcgtt
     2761 gcgctatctg gacaatgacg cggtccggtt gaccgacctg ctggccagga tgctggcgaa
     2821 gcgtgaccag tggctgcatc acaccacgca tgtgcaggaa gatgtggaga tggcgctaag
     2881 gcagttgatc cagcaggata ttgcccaggc cgcatcgtta ttgtccccgg atctgcagca
     2941 acggctgatg ccggttgcac ggtttgcagc ttccaatctg gagtgcaacc acctgatctc
     3001 gctgctgctg gattgggatt cgaccatcct ggccaggccg gaagcgctgc cgctgtggcg
     3061 cggcgtgtgc gaattgctgc tgaccagtgg cgatgacttc cgcaaggcgc tggacaagcg
     3121 gcagggattc ccggctaccg acgatggccg caaacagaaa gaggcgctgc tggagatcat
     3181 cgccatgctg ccgtcgccgc agcccttggc gcgcattcgc tgcctgccgg atgcgcaatc
     3241 ggaagaagaa aagcgcatcg tggcagcgct cgcccgcctg ctgcagctgg cggccgcgca
     3301 tctgctgata gtgtttcagg aaacgggcga ggtggatttt gtggaggtcg cgggccgtgc
     3361 gctggcggcg ctgcaagacg acgatggcgt gacggacttg gcgctgaagc tggattacaa
     3421 gattcaacat ctgctggtgg acgagttcca ggacaccagc ccgatgcaag tggaactgct
     3481 gcgccgcatg acgcaaggat ggctgcccga tgatggccgc accctgttct gcgtcggcga
     3541 cccgatgcag tcgatctacc gtttccgcaa ggcagacgta ggcttgttcc tgcaggtggc
     3601 gaactacggc atcggcgatt tgcagttgca acgcttgcat ctgacacgca acaaccggtc
     3661 ctgtccggcg gtagtggact gggttaattc ggcgtttgaa aaaatgttcc ccaaacagga
     3721 tgatgaagag cgcggagcca tacgctatcg tccgtttgct gcgaccaaag catcagaacc
     3781 caacgagggc gtgctggtac attcactggt gttgggggcg gacagccgcg gcgatgatat
     3841 ggccgcggaa gaagcgcgct tcctgacggc gttgattgcc agggaacgtg ccctcgaccc
     3901 caaggccagc attgccgtgc tggtgcgcgc cagggcgcac ttggaagcgc tggtggtgga
     3961 aatacgcagc cgccacccgg atttaagatt ccaggcagtg gaaatcgaac agctcggcgg
     4021 caggcagacg gtgcaggata tccatgcgtt gacctgcgcc ttgttccacc gtgccgaccg
     4081 cgtgcactgg ttggcgattc tgcgcgcccc ctggtgtggc ctgacgctgg ccgatctgca
     4141 catgctggcc gcggacgacc actacgccaa catctggcaa ctgatgcagg atgaggcgcg
     4201 gctttccaga ctttccgatg acggccgtaa gcgcctgctt catgttcgcg acgtgatcgc
     4261 cgaagcctat gcgcaccagg gccggcagcc catgcgccgc tgggtggagt cggtctggct
     4321 caggctgggt ggcgctgcat gcctgtggga tgcgggcgat gtgcgcgatg tgcaggcctt
     4381 cctcgatttg ctggagcgca tggaggtgtt cgatgcggcg caattgcaaa gcagaatgga
     4441 aaaactctat gccgcgccgg atgtgcaagc cgacgaaagt ttgcagttca tgaccatcca
     4501 caaatccaag gggctggaat tcgacaccgt catcctgccc gggctgcacc gcacttccaa
     4561 gaacggcgat ccacaattgc tgctgtggga ggaggtggcg atcgagggca cgccttcgca
     4621 gctggtagcg gcgccttatg tgccaaggca caagcgcgac gacctgccga gcacttatga
     4681 ctatctgcaa aacctggaac gggagcgcga gtccaacgaa accactcggg tgctgtatgt
     4741 ggcggcgacc agaacccggc ggcggctgca cctggtgggg gcagtgaggc cggacggcaa
     4801 gggtgaaatc aaggcgcccg ccaatagctt gctcagactg ctgtgggagt ccgtgggggg
     4861 cgagtttctc aatgcggagc cgcgcgctga gattgttgcg acggaaactt ccagcgcgtt
     4921 catccccagc ctggtccgcc tgccgcaggt ggcggttccg gaactgctgc agtcagggac
     4981 gcctgccgct gtgcgtccgg cgtttgacga agcagcgacg gacacaaaca tcaggagcat
     5041 cgacaccagt tgtggcacgt tggtccatct ctacctggaa atgatggcgg atgacggcct
     5101 gcaagactgg tcttcgcagc gcctgcatgg tctgcagttc gccatgcaaa cgtggctgca
     5161 agggcatggg catgcgcggg acgaggcgcg gcagggggcg gaaagggttt tgcatgcttt
     5221 atgccacacc ttggattccg aagccgggca atgggtgctc gcaagccggg ggagtgctgc
     5281 ttcggagctt gcgctggcga cggccgatgg ttcgcaattt gccacctacg tgatcgaccg
     5341 tacttttgtc gaagatggcg tacgctgggt gatcgactac aagtctgccc gtctggatac
     5401 tgtggaggaa gatatcctaa ctcgcgaggc cgcgcgctac cggccgcaac tcgcgcgcta
     5461 cgcggcgctt ttccgggaag aaggattgcc gatccgcatg gcagtgttct ttgtggccta
     5521 tgggaagctg gtggaactgg attgaggtag cgcagacaca ctcgcctgca taagccccac
     5581 cccaaccctc tcccagtggg agagggtaca aatgcagcag gcaaggacgc ttgtgctacg
     5641 tccgaactac ttctgtttga cttcgatcgg ctgcgcaggc gccgggtagc cggcctcgcc
     5701 gaacgcgtcg cgaatcgcta cattggtgtc gaaatacacc tgccagtagt ggtcgttatg
     5761 gcaataaggc cgtactgcca gcaccgggcc ggcggcggtg aattccagca attccaggtt
     5821 cggcgccggg tcctgcaata cattggcaat gccgtcaatc ctcgttttca gtcttgcaat
     5881 cgcatcgcgg tgatccacgc taccgttcag ctgcgctttg atatcgacgc ggcggtaggg
     5941 gttcgcggtg aagttctgga tggtgtcggc aaagatgcgg ctgttgccga cggtggtcag
     6001 cacgttatcc ggggtgttga tggcggtggc aaacaggccg atttccttga tggtgccggt
     6061 gatgccgccg gcggttacga agtcgcccac cttgaacggc cgcaacacga tcaggaacgc
     6121 acccgccgcg aagttggaca gcagaccgcc ccaggcggcg ccgatggcga taccgatacc
     6181 ggcgatcagc gcggcgaagc tggtggtctc gacgccgaaa taggacagga tggcgagggc
     6241 gagggcaatg ttcagcgtga ccgcaacaat cgagccgatg tagcgcaaca gggtgggatc
     6301 gaccttttgc cgctccaggc ccttgcgcac tatttcccca atcaggccga tgatccagcg
     6361 gcccaccacc cagaacacga tggcggcgac gatttgcagc ccgacatgga ttgcggtggt
     6421 ttgtacctgt tgcaggattt cctggatgtt catgttattt ttcccctagt tgaaaagaac
     6481 tacgtagtcg cgcaagcgcc tggcggctga tagaaaatcg aacggcaaat atagcacttt
     6541 ttcccggcgc aacaggaatc aatgatgatg cccgcgcacg gcgcggatat gccgccgtac
     6601 ataccacacc acgaaaatga ccgcggcggc ggcgatgacg tagtggaact cgtggaaata
     6661 caccttcagc ccggtccagt tctcgcccag cttcataccc agccaagcca gcacccagca
     6721 ccagatcagc gagccggcga aggtgtaggc gatgaagcgc cccatgtgca tgcgggcgat
     6781 cccggcgggg aaggcgatga aagtccgcac cgccggcaac aggcggccga tgaagatgat
     6841 gatctcgccg tgcttttcga accagcgatc ggcgatatgc aggtcgtggt gcgaaatcag
     6901 cacccatttg ccgtactttt ccaccagcgg acggccgcca tacatgccca ggtaataggc
     6961 cgggatcgag cccagcacgc agcccacggc gcccgccagc gccacgcccc acagcgccat
     7021 ctcgcccttg aacaccaggt agccggagaa cggcatgatg atctcggaag gcagcgggat
     7081 gcatgccgat tcgatgccca tcagcagcac gatgccgcca tagcccagcg tggaaatgac
     7141 ggcgatgatc cagccggcga ggatagcgat gagtttttcc agcatttagg caagatcctt
     7201 taacaaagtt ttgatgaact cggcacgctg caggatgttt tcctttgcaa ttgtaacgcg
     7261 cagtttgtcc ggcccgttca tgcgcgcgtg gcggttggtt tgcagcagcc tgaccagctt
     7321 catcgggtcg aggtcggcct gcaggtcgaa ctgcaactgg atggcggcgt cggaggcgtc
     7381 gatcttgacg atgcccatgg gtttcgcgtt gatgcgcagc ttgtggcatt ccagcagcgc
     7441 ctcggtctgc ggcggcggca ggccgaagcg gtcgatcaat tcttcctgca tcgcctgcaa
     7501 ctggtcgccg gtggtgcagt tggcgaggcg cttgtagatc accagccgct cgtgcacgtc
     7561 cgggcagtaa gcgtccggca gcaaggccgg ggtgtgcagg ttgatctcgg tggtgacgcc
     7621 cagcggcgca tccaggttgg gctccttgcc ggcctgcagc tgcttgacgg cgtgcgccag
     7681 catttcgctg tacaggttga agccgatttc ctgcatctcg ccggactgcg aatctcccaa
     7741 cagctcgccg gcaccgcgaa tttccaggtc gtgcatggcg aggtggaagc cggcgccgag
     7801 gtcttccatc aactggatgg cgtcgagtcg cttttgcgcc tgcggcgtga ccttcacgtc
     7861 cggctcggtg agcagatagg cgtaggcctg gtggtgcgag cggccgacgc ggccacgcag
     7921 ctggtgcaat tgcgccaggc cgaagcggtc ggccttgttc atgatgatgg tgttggcggt
     7981 cggcacgtcg atgccggtct cgatgatggt ggaacagagc agcaggttga agcgctgatg
     8041 gtagaagtcg cgcatcacgt gttccagctc gcgctcgcgc agctggccgt gggcgatgcc
     8101 gatgcgcgcc tcgggcagca tcttctccag ccggtcgcgc atggcgtaga tgctttccac
     8161 ttcgttgtgc aggaaataca cctggccgcc gcgcttgagt tcgcgcatca ccgcctcgcg
     8221 gatcacgcct tccgaccagg gcgagacgaa ggtcttgatg gaaagccgtt tctgcggcgg
     8281 cgtggcgatg acggagaact cgcgcagccc ttccatcgcc atcgacagcg tgcgcgggat
     8341 cggcgtggcg gtcagcgtca gcacgtccac ctcggcgcgc atggccttga gctgctcctt
     8401 ctggcgcacg ccgaagcggt gttcctcgtc caggatcacc aggccgaggt tcttgaaatg
     8461 gacatctttt tgaatgagcc ggtgggtgcc gatgatgatg tcgatgctgc cctcggccag
     8521 ccctttcagg gcttccgttt gctccttggc ggtgcggaag cgcgaaatct cggcgatctt
     8581 caccggccag tcggcgaagc ggtcggtgaa gttgttgtaa tgctgctccg ccagcagcgt
     8641 ggtcggcacc agcaccgcca cctggcgccc gcccatgacg gcgacgaagg cggcgcgcag
     8701 cgcgacctcg gttttgccaa agccgacgtc gccgcatacc agccggtcca tggggcggcc
     8761 actctgcatg tcgttgatga cggcctcgat ggcggccagc tggtccggcg tttcctcgaa
     8821 cgggaagttc tccgcaaacg cttcgtagtc gtgcaggcca agcttgaagg catggccctt
     8881 gcgcgcggcg cgcatggcgt agaggttgag cagttcggcg gcggtgtcgc ggatctgctt
     8941 gagcgcgcgc ttgcgcgcct tgtcccactg gccgctgccc agccggtgca gcggcgccga
     9001 ttccggcggg ccgccggagt agcgcgaaat caggaacagt tgcgataccg ggacatagag
     9061 cttgtcggga ggtgagtcct tcgcgggggg ggcgtattcc agcagcagga actcggtttc
     9121 gccctcgccg aaatcgaggt tgatcaggcc gcggtagcgc ccgacgccat gctgctcgtg
     9181 caccaccgga tcgttctcgc gcagctcgga caagtccttg agcatgccct cggtgctgcg
     9241 cgcgcggtct ttttcgcggc ggcgctcctg gcgcacctgg ctcgggtaga gttcggcttc
     9301 ggtgatgaag gcgatgtcgt cggagcgaaa tccgttgtgc agcggcgcca ccaccagcat
     9361 cagcggctcg ccggatgcgg aaaactgcgc ccagtcctcg cacggcttgg gccgcaggcc
     9421 gtgcgaggcc agcaactgca tcatggtttc gcgccgcccc aggctctcgg cagcgatcag
     9481 ggcacggccg tcgaagtctt gcaggaagcc ttgcagcctg tgcagcggaa gttcggaacg
     9541 gtgttcgata ttgaggtcgg gcagggacgg ggcgccttcc tccagtgaca gttgcagtcg
     9601 gccaaagcca tgagtggcgg caaaaaacgc gtccaccggc accagcaaac tttgcggtgg
     9661 caggatgggg cgttcacggt cgtggtcaag caggcggtgg cgggtttgcg tttcctgcca
     9721 gaaggtttgc gctgcctggt ccagttcgcc gtgcaggcat agcacggcat ccttcggcag
     9781 gtagtcgaac agcgttgcgg tctgctcgaa aaacaagggc aggtaccatt cgatgccgcc
     9841 cggtgcgatg ccgttgctga tgtccttgta gatgcggcat ttggaggggt cgccctcgaa
     9901 ggtctcgcgg aaggattgcc tgaatttcgc gatgcctttg tcgtccagcg ggaattcgcg
     9961 tgccggcagc aggcggattt ccgccaccgg gtacagactg cgctgggtgt cgacgtcgaa
    10021 agtgcggacg gtctcgatct cgtcgtcgaa caggtcgagg cggtagggca gcgcgctgcc
    10081 catcgggaac aggtcgacca ggccgccgcg cacgctgaat tcgcccggcg acatcacctg
    10141 gttgacgtgg tggtatccgg cttcggcgca ctgcgcgcgc aatgcctcca ggtcgagctt
    10201 ctgccctttc ttcagcatga aggtgtgcgc cgccaggaag gcctgcggcg gcagtcgcac
    10261 cagcgcggtc ccgaccggga tgatgatgac gtcgcaggcg ttctgcgtga tctggtagag
    10321 cgtcgccagc cgctccgaca ctaggtcggg gtgcggcgag aaatggtcgt aaggcagggt
    10381 ttcccagtcc ggcagcaggt ggatggccag ctgcggctcg aaccagcgca tttcctccag
    10441 caggcgctgc gcatcgaagg cgctggcgct gaggatcacc agcggctttt tcagggtgcg
    10501 ggcgagccgg gccagttcca ggctgtcggc accgcggcgc aggtcggaaa cgcgttctgt
    10561 tttacccgga gcagggatgg gcaggtgggc ttgcatagtg gggacgaatt atagccgtcc
    10621 ggcagttgct tccaaaataa aaaccccggc tcgctttcac gcg
//
LOCUS       DAOMFA010000047        10638 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_654_length_10638_cov_5.470755, whole genome shotgun sequence.
ACCESSION   DAOMFA010000047 DAOMFA010000000
VERSION     DAOMFA010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 10638)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10638)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10638
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_654_length_10638_cov_5.470755"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..464
                     /gene="bioD"
                     /locus_tag="PKW44_03710"
     CDS             <1..464
                     /gene="bioD"
                     /locus_tag="PKW44_03710"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HQR50724.1"
                     /translation="NPYAFEPALAPHIAARQGGVVIELMAVREAFMAAAQEVEALVVE
                     GVGGFRVPLNDIEDTADMAVLLGLPVILVVGMRLGCLNHALLTAEAIASRGLVLAGWV
                     ANRVDPTMSAFEDNLAALRQRLSAPCLGVLPHLEPADFRVAAERLDFDALR"
     gene            complement(519..899)
                     /locus_tag="PKW44_03715"
     CDS             complement(519..899)
                     /locus_tag="PKW44_03715"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HQR50725.1"
                     /translation="MQRTMLKSKLHRVRVTHSELEYEGSCAIDEELLDAADIREYQQI
                     EIYNVNNGERFTTYAIRAQRNSGIISVNGAAARKAAPGDILIIATYAAYNELELEKFK
                     PDLVYVDAMNRIREKRRDIPTQAA"
     gene            complement(934..1764)
                     /gene="panC"
                     /locus_tag="PKW44_03720"
     CDS             complement(934..1764)
                     /gene="panC"
                     /locus_tag="PKW44_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HQR50726.1"
                     /translation="MDIISAIPELRSRLANAGRIAFVPTMGNLHAGHIALAKLARGRG
                     DCAVASIFVNPLQFGQGEDFAAYPRTLEQDCAKLAEAGCDVVFTPSEAVLYPQPQTLY
                     VEPPAIADELCGAHRPGHFRGVCTVVAKLFNIVQPQVAIFGNKDFQQLHVLREMARQL
                     DFPIEIVAGETVRETDGLAMSSRNGYLKPEQRMEAPRLYRALQQVVQAIQSGRHDYAA
                     AQAQTAQYLTQLGWIVDYIEVRNADTLSKPAPTDTRLVVLGAARLGKTRLIDNIEFAI
                     "
     gene            complement(1764..2543)
                     /gene="panB"
                     /locus_tag="PKW44_03725"
     CDS             complement(1764..2543)
                     /gene="panB"
                     /locus_tag="PKW44_03725"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018076323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HQR50727.1"
                     /translation="MTGLDALQAMKTNGEKIAVLTCYDASFATLMAQAGVDALLVGDS
                     LGMVLQGAGDTLSVAMRDMEYHTRCVAAGAKSTPVVADMPAGSDRTPGTALDNARRLI
                     EAGAHMVKLEGCKTEVVRHLAQHHIPVCGHLGFTPQSVYELGGYKVQGRDEAGARQIL
                     EDSLALQQAGAGLLVLEMVPAALAATVTAALAIPTIGIGAGPDCDGQVLVLHDMLGIY
                     PGKTPRFARNFLQGAGSVQTALTAYVQAVKDKTFPAAEHSF"
     gene            complement(2540..3187)
                     /locus_tag="PKW44_03730"
     CDS             complement(2540..3187)
                     /locus_tag="PKW44_03730"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HQR50728.1"
                     /translation="MNLTEKYPYIVIEGPIGCGKTTLARKLADRFPVNLLLENASANP
                     FLPRFYEDVARYALPTQLFFLFQRAEQVRELSQRDLFDKATIADFFLEKDPLFARLTL
                     RDEEFALYRQIFQHLQLQTTVPDLVIYLQTPVDALLERIEQRNINYEQGISKEYLARL
                     ADGYSEFFHQYDASPLLIVNNANLNCAASEEALDLLLERIGKIHSRREFFNPDLA"
     gene            complement(3184..3669)
                     /gene="folK"
                     /locus_tag="PKW44_03735"
     CDS             complement(3184..3669)
                     /gene="folK"
                     /locus_tag="PKW44_03735"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HQR50729.1"
                     /translation="MSHRAFIALGSNLQDPAAQVKRALQELAEVPGTQLIRASSLYRT
                     APMGYDRQPDFINAVAEIATMLKPLPLLHALLALEDGHGRERPFPNAPRVLDLDLLLY
                     DDLTMETPELTLPHPRMSARGFVLLPLAEIAPELPIPEHGKAADLALQCLDQGVERLT
                     A"
     gene            complement(3666..5012)
                     /gene="pcnB"
                     /locus_tag="PKW44_03740"
     CDS             complement(3666..5012)
                     /gene="pcnB"
                     /locus_tag="PKW44_03740"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004652 - polynucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006378 - mRNA polyadenylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HQR50730.1"
                     /translation="MIKKLLQRVFKGKKKTVTPDASHSVKRFPRRVHGIDRKAISHAA
                     LKTTQELQKAGFAAFVVGGAVRDLILRRTPKDFDIATDATPEQVHRIFRRSRLIGRRF
                     RIVHVMFGPETVEVTTFRGSHLSDDGDASIADSGRILRDNVFGTMEEDAARRDFSANA
                     LYYDPQTEEVLDYHHGLPDIQAGKLRIIGHAETRYREDPVRMLRAVRLSAKLGLKLDP
                     KTEAPIPHMADLLQDVPPSRLFDQMLKLFLSGHAMDSANALRAHGLHHGLLPLLDVVL
                     EQPMGERFVTLALQNTDERVLQDKPVSPAFLFAALLWHEVLAAWQKYQDNGERPMPAL
                     VEAMHDVSDIQAEKLAITKRFAVNMKEIWSLQPRFEQRSGQRPYRLLEHPRFRAGYDF
                     LLLRCQSGELPMELAEWWNNFAQTDADQRQEMLLPNTEPAKKRRRRRRKPATAPEA"
     gene            5123..5929
                     /gene="murI"
                     /locus_tag="PKW44_03745"
     CDS             5123..5929
                     /gene="murI"
                     /locus_tag="PKW44_03745"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HQR50731.1"
                     /translation="MNASIANAPVGVFDSGVGGLSVLHHIRQQLPQETLLYCADSRYA
                     PYGNKTPGQIRERSLRLTEFLLGQGAKAVVVACNTATAAAVAELRLRFSVPIIAMEPA
                     VKPAAAASRSGVIGVLATVGTLKSAQFAALLENYGKEVEVVTQACHGLVECVERGALD
                     SPETRDLVRSYVQPLLAHGADTLVLGCTHYPFLRGLIEQAAGPGVTLVDTGAAVARHL
                     RRRLEEAGLLVLAGSAGEHFWTTGDLGQARAIMPRLWGVDVLVERLPETP"
     gene            complement(5960..6151)
                     /locus_tag="PKW44_03750"
     CDS             complement(5960..6151)
                     /locus_tag="PKW44_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HQR50732.1"
                     /translation="MANTPREVEVTAKDLPLHCPTAETALWCSHPRVFLDIAASGEAF
                     CPYCGTKYRLKAGESVKSH"
     gene            complement(6162..7076)
                     /locus_tag="PKW44_03755"
     CDS             complement(6162..7076)
                     /locus_tag="PKW44_03755"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HQR50733.1"
                     /translation="MADRDGLIWYDGKMVPWRDATTHVLTHTLHYGMGVFEGVRAYKT
                     EKGTAIFRLKEHTDRLFRSAHILGMKMPFDKATLMEAQKAAVRENHLESGYLRPMAFY
                     GAEAMGISARTLSTHVIVAAWAWGAYLGKEALENGIRVKTSSFARHHVNITMCKAKAN
                     GNYMNSILAHQEAAHDGYDEALLLDVDGFVAEGSGENVFIIRNGKLYTPDLTSALEGI
                     TRDTIVQLAGEIGLQVIEKRITRDEVYSADEAFFTGTAAEVTPIRELDNRAIGAGKRG
                     PVTEKLQAMFFDVVTGKSPAHAGWLTLV"
     gene            7159..8007
                     /locus_tag="PKW44_03760"
     CDS             7159..8007
                     /locus_tag="PKW44_03760"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HQR50734.1"
                     /translation="MRVLLVGTVTLDLVFGLEHHPAADEEMRAASLRTCRGGNAANTA
                     VVLARLGHAPEFLGVLADAPETAVIERDFAMHGVAYGRCPRLTGRPPTSSIYLSGATR
                     SIVHYRDLPELDAAYFTELDLAAYDWLHFEGRNVPALVRMLAYVRAKFPGLPVSLELE
                     KPRDGLEDLFGQADVLLCSRGLARHYGFMEAQAFLGWMQGQAPRSTVVAAWGDAGAYG
                     LAPGGQACHAPALVPVRVMDTLGAGDTFNAGIIDAGIREMPLQRMLTDACQLAGRKCG
                     AEGYMF"
     gene            8158..8880
                     /locus_tag="PKW44_03765"
     CDS             8158..8880
                     /locus_tag="PKW44_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpW family outer membrane protein"
                     /protein_id="HQR50735.1"
                     /translation="MKKTLLAVMMAAAFAPAVAMAEQGDFVVRLRATNISPAVNSHLG
                     QQTDQTYGAGTANLLYGSSTANLEVESNTIPEVDFSYYVTNNIALELILALGSRHDVN
                     VSGSGGAVNNSLGDVNLLPPTLTAQWHFMPDQKFDPYVGAGVTYVRSLDTGLKADAGA
                     AGTYPIRIDHNMWGPAVQLGFDVNMQDRWLVNFDVKKIWLETDVKVNLDGNPLGAPGG
                     YKKIDELQLNPWVVSFGVGKKF"
     gene            8987..9595
                     /locus_tag="PKW44_03770"
     CDS             8987..9595
                     /locus_tag="PKW44_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HQR50736.1"
                     /translation="MKSPKKMHCWLAFMLCMCLAACGRGQPGLGSFVGTDVSGAAFGG
                     TLELTDHHGQARTLADFRGKLVALFFGYTHCPDVCPTTMDDLAKALKLLGGRRDEVQV
                     LFVTLDPERDTQQVLAQYVPAFDPGFLGLYGSPEAIAATARNFKVFYQKQFSGSKSGY
                     SVDHSAGVYVFDKKGQLRLYLNYGQKPQDIAHDFGTLLSEEK"
     gene            9712..>10638
                     /locus_tag="PKW44_03775"
     CDS             9712..>10638
                     /locus_tag="PKW44_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011310851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HQR50737.1"
                     /translation="MATASAVAGEQYNYDIVRKFTIMTLVWGTVGMLVGVIIASQLAY
                     PVLNFDIPYITFGRLRPVHTGAVIFGFGGSALFATSYYIVQRTCQARLFGTGLANFTF
                     WGWQTIIVLAALNHTLGFSQGREYAEMEWPIDLLIAVVWVSYAVVFIGTVMKRKEPHI
                     YVANWFYMAFILATALLHIFNNLAIPVSLTKSYPLFSGAQDAMTQWWYGHNAVGFFLT
                     AAFLGMMYYFVPKQAGRPIYSYRLSIVHFWALIFLYMWAGTHHLHWTAIPDWTSTLAA
                     TFSIMLLLPSWGGMINGIMTLSGAWDKLRTDPI"
BASE COUNT         1858 a         2978 c         3507 g         2295 t
ORIGIN      
        1 ccaatcccta cgccttcgag ccggcgctgg cgccgcacat tgccgcccgg cagggcggcg
       61 tggtcatcga gctgatggcg gtgagggagg cgtttatggc agcagcacag gaagtcgagg
      121 cgctggtggt ggagggcgtg ggcggcttcc gcgtgccgct caacgatatc gaggataccg
      181 ccgatatggc ggtactgctg gggctgccgg tgatcctggt ggtgggcatg cgactgggtt
      241 gtctcaacca cgccttgctg acggcggaag cgattgcctc ccgcggcttg gtgctggcag
      301 gctgggtggc taaccgtgtt gatccaacga tgtcggcttt cgaggacaat cttgccgctt
      361 tgcgtcagcg gctctccgct ccctgcctcg gcgtcctgcc ccatctggag ccggcagatt
      421 ttcgggtagc ggcagagcgt ctggatttcg acgccttgag atgaccgaag acggaaacaa
      481 aaaaggcagc cttgactgcc ttttttgccg agagatggtc aggcggcctg cgtcgggata
      541 tcgcggcgct tttccctgat acggttcatc gcatctacgt acaccaggtc cggcttgaat
      601 ttttccagtt ccagctcgtt gtaggcggcg taggtggcga taatgagaat gtcgcctggc
      661 gcagccttgc gcgcggcggc gccgttgacc gaaatgatgc cggaattgcg ctgggcgcgg
      721 atggcgtagg tggtgaagcg ttcgccgttg ttgacgttgt agatttcgat ctgctggtac
      781 tcgcggatgt cggcggcgtc cagcagttcc tcgtcgatgg cgcaggaacc ctcgtactcc
      841 agctcggaat gcgtcacgcg cacccggtgc agcttggatt tgagcatggt tctttgcatg
      901 gcttgtctcg gcgtttcgaa aaaaggacga ttattatatc gcaaactcga tattgtcgat
      961 gagtctggtc tttcccagcc gcgccgcgcc gagtactacc aggcgggtgt ctgtcggggc
     1021 tggcttgctc aatgtgtcgg cgttgcggac ctcgatatag tctacgatcc agcctaactg
     1081 ggtcaaatat tgcgcggttt gcgcctgggc tgccgcgtaa tcgtgccgtc ctgactggat
     1141 ggcctgcacc acctgctgca gggcacgata caagcgcggt gcctccatgc gctgctccgg
     1201 cttcagatag ccgttgcgcg agctcattgc caggccgtcg gtttcgcgca ccgtttctcc
     1261 ggccacgatc tcaatgggga aatcaagctg gcgcgccatt tcgcgcagca cgtgcagttg
     1321 ctggaaatcc ttgttgccga agatcgcgac ctgcggctgt acgatgttga acagcttggc
     1381 caccacggtg catacgccgc gaaaatggcc cgggcggtgg gcgccgcaca gctcgtcggc
     1441 gatggccggc ggttcgacat acagggtctg cggctgcgga taaagcactg cctcgcttgg
     1501 cgtgaacacc acgtcgcagc cggcctcggc cagtttggcg cagtcttgct ccagggtgcg
     1561 cggataggcg gcgaaatcct cgccctggcc gaattgcagc gggttgacga agatgctggc
     1621 caccgcgcaa tccccacgcc cgcgtgccag tttggccagt gcgatatggc cggcatgcag
     1681 gttgcccatg gtgggaacga aggcaatgcg gcccgcattg gcaaggcggc tacgcagttc
     1741 gggaatggca gaaatgatgt ccatcagaag ctgtgttctg cggcgggaaa agtcttgtcc
     1801 tttaccgcct gcacgtaggc ggtcagcgcg gtttgcacgc tgccggcccc ttgcaggaag
     1861 ttgcgtgcga agcggggcgt cttgccgggg tagatgccca gcatgtcgtg cagtaccagc
     1921 acctggccgt cgcagtctgg ccccgcgcca atgccgatgg tgggaatggc gagcgcggca
     1981 gtgaccgtgg ccgccagcgc agcaggcacc atttccagca ccagcaggcc ggcgccggcc
     2041 tgctgcaggg ccaaggagtc ctccaggatt tgcctggcgc cggcctcgtc gcggccttgc
     2101 accttgtagc cgcccagttc gtacacggac tgcggggtga agcccagatg cccgcatacc
     2161 gggatgtgat gttgcgccag gtggcgtact acctcggttt tgcaaccttc cagcttgacc
     2221 atgtgggcgc cggcttcgat caggcggcgt gcgttgtcca gggctgtgcc gggcgtgcgg
     2281 tcgctgccgg ccggcatgtc ggcgacgacc ggcgtggact tggcgcccgc cgccacacaa
     2341 cgggtgtggt attccatgtc gcgcatggcg acacttaagg tgtccccagc gccttgcagc
     2401 accatgccca gcgaatcgcc caccagcagc gcgtccacgc cggcctgcgc catcagcgtg
     2461 gcaaagctgg cgtcatagca ggtcaaaacg gcgatcttct cgccgttcgt tttcatggcc
     2521 tgcaatgcgt cgaggccggt catgccaggt ccgggttgaa gaattcccgg cgactgtgga
     2581 tcttgccgat gcgttccagc agcaggtcga gcgcctcctc gctggcggcg cagttgaggt
     2641 ttgcgttgtt gacgatcagc agtggcgagg cgtcgtactg gtggaaaaac tcgctgtagc
     2701 catctgccag tctggccagg tattccttgg aaatgccttg ctcgtaattg atgttgcgct
     2761 gctcgattcg ctccagcagg gcgtccaccg gagtttgcag gtagatcacc agatcgggta
     2821 cggtggtttg cagttgcagg tgctggaaga tctggcggta gagggcgaat tcctcgtcac
     2881 gcagggtcag gcgcgcgaac agcgggtctt tctccaggaa gaagtctgcg atcgtggcct
     2941 tgtcgaacag gtcgcgctgg ctgagttccc ggacctgttc ggcacgttgg aacaggaaaa
     3001 acagttgtgt cggcagcgcg tagcgtgcga catcttcata gaagcgtggg agaaaggggt
     3061 tggcgctggc gttttccagc agcaggttga ccgggaaacg gtcggccagc tttcgggcga
     3121 gggtggtctt gccgcagccg atggggccct cgatgacgat gtaggggtac ttttcggtca
     3181 ggttcacgcc gttagccttt ccacgccctg atcaaggcac tgcagtgcga ggtcggccgc
     3241 cttgccatgc tccggtatcg gcaattccgg ggcgatttcc gccagcggca gtagcacgaa
     3301 gccacgcgcg gacatgcgcg ggtggggcag ggtgagttcc ggcgtttcca tggtcagatc
     3361 atcataaagc aaaaggtcga gatcgagcac tcttggggca ttgggaaacg ggcgctcgcg
     3421 accgtggccg tcttccagtg ccagcagggc gtgcagcagc ggcaacggct tcagcatggt
     3481 ggcaatctct gctacggcgt tgataaagtc cggctggcgg tcgtagccca tcggtgcggt
     3541 gcggtagagc gaagaggccc tgataagttg ggtgcccggc acctcggcaa gctcctgcag
     3601 tgcgcgtttc acctgtgcgg cagggtcttg caggttgctg ccaagggcga tgaaggcccg
     3661 gtggctcatg cttcgggggc ggtcgccggt ttgcgtctcc ggcggcggcg ctttttcgcc
     3721 ggttcggtat tgggcagcaa catttcctga cgctggtcgg catcagtctg ggcgaaattg
     3781 ttccaccatt cggccagctc catcggcagt tcgccagact ggcagcgcag cagcaggaag
     3841 tcgtagccgg cgcggaaacg cgggtgttcc agcaggcgat aggggcgctg gccggagcgc
     3901 tgttcaaagc ggggctgcag gctccagatt tccttcatgt tgacggcgaa acgcttggtg
     3961 atggccagct tctcggcctg aatgtcggag acgtcgtgca ttgcttccac cagcgccggc
     4021 atcggccttt cgccgttatc ctggtatttc tgccaagccg ccagcacctc gtgccacagc
     4081 agggcggcga acaggaaggc gggcgatacc ggcttgtcct gcagcacccg ttcgtcagtg
     4141 ttctgcaagg ccagggtgac gaagcgctcg cccatcggct gctccagcac cacgtccagc
     4201 agcggcagca ggccatggtg caggccgtgg gcgcgcagcg cgttggcgct gtccatggcg
     4261 tggccggaga ggaacagctt gagcatttga tcgaacaggc gcgacggcgg cacgtcctgc
     4321 aacaggtcgg ccatgtgcgg gatcggcgct tcggtcttgg ggtcgagctt cagtcccagc
     4381 tttgcggaaa gccgtacggc gcgcagcatg cgcaccgggt cttcgcggta gcgggtctcc
     4441 gcgtggccga tgatacgcag cttgccggcc tggatgtccg gcaggccgtg gtgataatcc
     4501 aaaacttcct ctgtctgcgg gtcgtaatac agcgcattgg cgctgaagtc gcgccgcgca
     4561 gcatcctctt ccatggtgcc gaatacatta tcgcgcagga tgcggccgct gtcggcgatg
     4621 ctggcatctc catcatcgga caagtggctg ccgcggaagg tggtgacctc gacggtttcc
     4681 gggccgaaca tcacgtgcac gatgcggaag cggcggccga tcaggcgcga gcggcggaaa
     4741 atacgatgca cctgctccgg cgtggcatcg gtggcgatgt cgaaatcctt gggcgtgcgg
     4801 cgcaggatca ggtcgcgcac cgcgccgccg accacgaagg cggcaaagcc ggctttttgc
     4861 agttcctgcg tggttttcag cgcggcatgg ctgatcgcct tgcggtcgat gccgtggacg
     4921 cggcggggaa atcgtttgac gctgtgggag gcatcggggg tgacagtctt ctttttaccc
     4981 ttgaagacac gttgcagcag ttttttgatc atggggacaa caaggctatg gggtggatat
     5041 caacccggct gattataacg catgtgccgt caggcaaggc cgccattgtc gcttgcgcat
     5101 acattccgct acactccggc gcatgaacgc atccattgcc aatgcgccgg tcggcgtgtt
     5161 cgattccggt gtcggcggcc tgtcggtgct gcaccatatc cggcagcagt tgccgcagga
     5221 aacgctcctc tactgtgctg attcgcgtta tgccccgtat ggcaacaaga cgcccggaca
     5281 aatccgtgag cgctcgctgc ggctgacgga atttttgctg gggcagggag ccaaggccgt
     5341 cgtggtggct tgtaacaccg ctactgctgc ggccgtggcc gaattgcggc tgcgtttcag
     5401 tgtccccatc atcgccatgg aaccagcggt caagccggca gcggcagcca gcagaagtgg
     5461 tgtgatcggc gtgctggcca cggtcggtac actaaagagc gcgcagttcg ccgctttgct
     5521 ggaaaattac ggcaaggagg tggaggtggt gacgcaggct tgtcacggcc tggtcgagtg
     5581 tgtcgagcgc ggcgcgctgg actcgcctga gacgcgcgac ctggtgcgca gctatgttca
     5641 gccgctgttg gcgcatggcg cggatacgct ggtgctgggt tgcacccact atcctttttt
     5701 gcgcggcctg atcgagcagg ccgccggccc cggggtgacg ctggtggata cgggtgccgc
     5761 ggtggcgcgg catttgcggc gccggctgga ggaggccgga ttgcttgttc tggcgggcag
     5821 cgccggggag catttctgga ctacagggga tttgggtcaa gcgcgcgcaa tcatgccgcg
     5881 actgtggggc gttgacgtgc tggtagagcg gttgccggaa acgccgtagc gcgggcggtc
     5941 ctgcccgcgc tacggaagat cagtggctct tgacgctctc gccggccttg aggcggtact
     6001 tggtgccgca gtacgggcaa aacgcctcgc cgctggcggc gatatccagg aacacgcgcg
     6061 gatgcgagca ccacagcgcg gtttccgccg tcgggcaatg cagcggcagg tctttggccg
     6121 tgacctcgac ctcgcgcgga gtgtttgcca tgagcgttcc cttaaaccag cgtcagccag
     6181 ccggcatggg cgggagattt gcccgtcacc acatcgaaga acatggcttg cagcttttcc
     6241 gtcaccgggc cgcgcttgcc tgcaccgatg gcgcggttgt ccagttcgcg gatcggcgtc
     6301 acttcggcgg cagtgccggt gaagaaggcc tcatcggcgg aatacacctc gtcgcgggtg
     6361 atgcgcttct cgatcacctg caggccgatc tcgccggcca gttggacgat ggtgtcgcgg
     6421 gtgatgcctt ccagcgcgct ggtcaggtcc ggcgtataca gcttgccgtt acgaatgatg
     6481 aacacgtttt cgcccgagcc ttcggccacg aacccatcta cgtccagcaa cagcgcttcg
     6541 tcgtagccgt cgtgcgcagc ttcctgatgg gccagaatgg agttcatgta gttgccgtta
     6601 gccttggcct tgcacatggt gatgttgacg tggtggcgcg cgaatgaaga ggtcttgacg
     6661 cgaataccgt tctccagtgc ctccttgccg aggtaagcgc cccaggccca ggccgccacg
     6721 atgacgtgcg tggacaatgt tctggcggaa atgcccatgg cttctgcgcc atagaatgcc
     6781 atcgggcgca ggtagccgga ttccagatgg ttctcgcgca cggcggcttt ttgcgcctcc
     6841 attagcgtag ccttgtcaaa cggcatcttc atgccgagga tatgtgccga gcggaacagg
     6901 cggtcggtat gttccttgag gcggaagatg gcggtgccct tctcggtctt ataggcgcgc
     6961 acgccctcga aaacgcccat gccgtaatgc agggtgtggg tgaggacgtg ggtggtggcg
     7021 tcgcgccaag gcaccatctt gccgtcgtac cagatcagac cgtcacggtc ggccatcgtc
     7081 ttggccatag ccatcttgtt tctccgaagc gtaaaaggtc taattgtaaa gggggaaggg
     7141 agatggggga agggtgtgat gcgggtactt ctggtgggaa cggtaacgct ggacctggtg
     7201 ttcggactgg aacatcaccc ggcggcggac gaggagatgc gtgccgccag cttgcgcacc
     7261 tgccgtggcg gcaatgccgc caataccgcc gtggtgctgg cccggctcgg acatgcgccg
     7321 gaatttctcg gcgtgctggc cgatgcgccg gaaacggcgg tgatcgaacg tgattttgcc
     7381 atgcacggcg tcgcgtacgg ccgttgtcca cgcctgacag gacggccgcc gacctccagc
     7441 atctatctca gcggcgccac ccgaagcatc gtgcattatc gcgacctgcc cgaactggac
     7501 gctgcatact ttacagaact tgatctggcg gcttatgact ggctgcattt tgaagggcgc
     7561 aacgtgccgg cgctggtccg gatgctggct tacgtgcggg cgaaattccc cggattgcca
     7621 gtttcgctgg agctggaaaa gccgcgtgac ggcctggagg atttgtttgg ccaggccgat
     7681 gttctgcttt gttcgcgtgg attggcgagg cattacgggt ttatggaagc gcaagctttt
     7741 ctgggctgga tgcaggggca ggcgcccagg tcaacggttg ttgcggcatg gggtgacgca
     7801 ggcgcctatg ggctggcgcc tggcggacag gcttgccatg cgccggcact ggtgccggtg
     7861 cgggtgatgg ataccttggg cgcgggggat accttcaacg ccggcatcat cgatgccggc
     7921 atcagggaaa tgccactgca gcggatgctg acggacgcct gccagctggc aggcaggaaa
     7981 tgtggggcgg aaggttatat gttttagaga acggctatct gatgttgcaa aaatgcgaca
     8041 catgtaattt tttctgatgg aagtttcatt taatgctttt gcagtcatgg gtaatgccgg
     8101 tagaattcga ttcaagagat ttttgtcctg taataaattt ttaatgggga gcatcaaatg
     8161 aagaagactt tactggcggt gatgatggca gcggcgtttg cacctgctgt ggcaatggct
     8221 gagcaaggtg atttcgtcgt gcgtctgcgt gccaccaata tcagtccggc cgtgaacagt
     8281 catcttggcc agcagactga ccagacctat ggtgcgggta cggcgaacct attgtatgga
     8341 agcagcactg ccaacctgga agtggaaagc aataccatcc cggaagttga cttttcctac
     8401 tacgttacca acaacatcgc gcttgagctg atcctggctc tgggtagcag gcatgatgtc
     8461 aacgtttccg gctcgggcgg tgcagtcaat aacagcttgg gcgatgtcaa cctgctgccg
     8521 cctacgctga ctgcacaatg gcacttcatg ccggatcaaa agttcgatcc gtacgtgggt
     8581 gccggtgtca cctatgtgcg ttccctggat accggtctga aggcggatgc cggtgctgct
     8641 ggcacctatc ccattcgcat cgatcataac atgtgggggc ctgctgtcca gcttggtttt
     8701 gacgtgaaca tgcaggatcg ctggctggtc aacttcgatg tcaagaaaat ctggttggaa
     8761 accgatgtga aggtgaatct ggatggtaat ccgcttggcg cacctggcgg ttacaaaaag
     8821 atcgacgaac tccaactgaa tccgtgggtc gtcagcttcg gcgtcggcaa aaagttctaa
     8881 gtcgccccgc cctgaccgac atatcacggc aaaccccctt ttccgcaggc cgggaagggg
     8941 gttttgtttt ggggatgcgt catcagcgcg tgataaaatc ctttcgatga aatccccgaa
     9001 aaaaatgcac tgctggctgg cattcatgct ttgcatgtgc ctagccgctt gtggcagagg
     9061 gcagcccggg ctcggcagtt ttgtcggcac ggatgtcagc ggtgcggcgt ttggcggcac
     9121 acttgaattg accgaccatc acggacaggc cagaacgctg gcggattttc gcggcaagct
     9181 ggtggccctg ttcttcggct acacccattg cccggacgtc tgcccgacca ccatggacga
     9241 cctggctaag gcgctgaagc tgctgggggg taggcgtgac gaggtgcagg tgctgttcgt
     9301 gacgctagat cctgagcgcg atacgcagca ggtactggcg cagtacgtgc cggccttcga
     9361 tcccggcttc ctagggcttt acggcagccc ggaggcgatc gccgcaactg ccaggaattt
     9421 caaggttttt taccagaagc aattctccgg cagcaagtcc ggttatagtg tcgaccatag
     9481 cgcgggtgtt tacgtgttcg ataaaaaagg ccagctacgg ctctatctga attacgggca
     9541 gaagccgcag gatattgccc atgatttcgg caccttgctg agcgaagaaa aataaacaag
     9601 acttacttgt cggattaggt ttggcggcat atacttgcgc ctgaggtgcg tcattctgtc
     9661 gcgtggtggg tggcgtgtcg tcaatcttgt gtacgtaaag gggaggtaac gatggcaaca
     9721 gcgagcgctg tggctggcga acagtacaat tacgatattg ttcgcaagtt cacgatcatg
     9781 accctggtat ggggtacggt ggggatgctg gtgggggtga tcatcgcctc ccagctggca
     9841 tatcctgttc tgaattttga tattccgtac atcacttttg gccgcttgcg gccagtgcat
     9901 acgggtgccg tgatcttcgg ctttggtggt agcgcgctgt ttgctacttc ttactacatc
     9961 gtgcaacgca cctgtcaggc gcgcctgttc ggcacgggtt tggccaattt caccttctgg
    10021 ggttggcaga ccattatcgt gctggcggcg ctgaaccaca cgctcggttt cagccagggg
    10081 cgtgagtacg ctgaaatgga atggccgatc gacctgctga tcgccgtggt gtgggtgtcc
    10141 tatgccgtgg tgttcatcgg cacggtgatg aagcgcaagg agccgcatat ctatgtggcc
    10201 aactggttct acatggcgtt catcctggcg actgcgctgc tgcacatctt caacaatctg
    10261 gcgattccgg tcagcctgac caagtcttac ccgctgttct ccggcgcgca ggatgcgatg
    10321 acccaatggt ggtacggcca caacgcggtg ggtttcttcc tgaccgcggc tttccttggc
    10381 atgatgtatt acttcgtgcc taagcaggcc ggccgcccga tttattccta tcgcctgtcc
    10441 atcgtccact tctgggcgct gatcttcctc tacatgtggg cgggtactca ccacttgcac
    10501 tggacggcca ttccagattg gacttcgacg ctggcggcga ccttctccat catgctgctg
    10561 ctgccttcct ggggcggtat gatcaacggc atcatgacgc tgtccggcgc gtgggataag
    10621 ctgcgtaccg atccaatc
//
LOCUS       DAOMFA010000048        10559 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_661_length_10559_cov_5.536938, whole genome shotgun sequence.
ACCESSION   DAOMFA010000048 DAOMFA010000000
VERSION     DAOMFA010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 10559)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10559)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10559
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_661_length_10559_cov_5.536938"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..788
                     /locus_tag="PKW44_03780"
     CDS             <1..788
                     /locus_tag="PKW44_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQR50738.1"
                     /translation="MATVLMLFGLAPAVAPVLGGVLLGIHWQAIFIFLALYAGLTLWA
                     AMRYLPETMPPEKRLPLSVRQVLKSYRTIFSDWEFGRLAIAVGANFSGFFLYVLASPV
                     FLVKHLGLNPHQFGWMFIPTVCGMVFGSYLAKRAAGRYSQQSVVRVAYAWMAAMVLLN
                     LAICFSLPANPLYNIAPVALFNIGMAFAMPVLSIAALDRHPGIRGTAASGQAFVQMFL
                     STVSAGLIVPLVWQHPSGLALAMVGYLLLGWGVIRSSKLWLGK"
     gene            complement(833..1156)
                     /locus_tag="PKW44_03785"
     CDS             complement(833..1156)
                     /locus_tag="PKW44_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019898753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HQR50739.1"
                     /translation="MTYVVTENCIQCKYTDCVDVCPVDCFVEGPNFLAIDPDECIDCT
                     LCVAECPVEAIFAEDDVPADQQEYIELNARLAKVWPAITAKKDALPDAEQYNGVTGKR
                     GLLIE"
     gene            complement(1197..2483)
                     /gene="cysN"
                     /locus_tag="PKW44_03790"
     CDS             complement(1197..2483)
                     /gene="cysN"
                     /locus_tag="PKW44_03790"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HQR50740.1"
                     /translation="MNAIDKNLFEAADTALLRFTTAGSVDDGKSTLIGRLLVDTKGVF
                     EDQLDAAKNVEDKKGAKGAHLALLTDGLQAEREQGITIDVAYRYFATPKRKFIIADTP
                     GHEQYTRNMVTGASSAHLAIILIDARKGVLTQSRRHAYISRLLGIPHIVVAINKMDLV
                     DYSQEIYEQIRQDFSDFYGKLGFIGGEVTFIPISALEGDMVVTRGDHMPWYEGQTLLE
                     KLESASVEGKLNAEDFRFPVQYVSRPQTEELHDFRGYMGRIESGSVKVGDAVTVLPSG
                     LTSKIKSIVTWEGELTEAYAPMSVTLTLEDERDISRGDMIVHSDNQPRVEKELSAMLC
                     WMHETPLQAGKKYLIKHATSTVKAVVTALEQRVDINTLEDIPAPPELKMNEIGKVGIK
                     LLKPIACDAYADNRATGGFIVIDDFTNMTVGAGMIQ"
     gene            complement(2483..3385)
                     /gene="cysD"
                     /locus_tag="PKW44_03795"
     CDS             complement(2483..3385)
                     /gene="cysD"
                     /locus_tag="PKW44_03795"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HQR50741.1"
                     /translation="MDNKTLTHLQTLEAESIHIMREVVAEFRNPVMLYSVGKDSSVML
                     HLARKAFHPGPLPFALLHVDTGYKFKEMYEFRDWYAKEIGARLIVHRNEEAIAQGAHP
                     LKLGVARCCGALKTKALLDALEEGGYDAAMGGARRDEERSRAKERIFSFRDEFGQWDP
                     KNQRPELWDIYNCRVHEGESIRVFPISNWTEMDIWQYIKLENIPIVPLYYAKERPMVK
                     RGAMLIPADDAGNLKPGEVPENVMCRFRTLGCSPCTGAVYSSADTIEQIVAESAVATR
                     SERETRIIDHGSNTMEDKKKEGYF"
     gene            complement(3388..4077)
                     /locus_tag="PKW44_03800"
     CDS             complement(3388..4077)
                     /locus_tag="PKW44_03800"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HQR50742.1"
                     /translation="MTLQAKIAAAAILLERIQDEFSPATLASSLGAEDMVLTDLIAKH
                     APGISIFSLDTGRLNPETYDLLAKVQETYGEKVLIRVLYPQPEPLQQYVQANGINAFY
                     HSVELRKACCAIRKVEPLKHALAGNKAWITGMRRQQAVTRADMPVQEFDADHGLAKFN
                     PLADWTEKEVWEYIRSNAIPYNALHDKHYPSIGCAPCTRAIAVGEDLRAGRWWWENEA
                     GKECGLHVKKI"
     gene            complement(4077..4739)
                     /locus_tag="PKW44_03805"
     CDS             complement(4077..4739)
                     /locus_tag="PKW44_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF934 domain-containing protein"
                     /protein_id="HQR50743.1"
                     /translation="MSNLIKNNAIVANDWTLVALPAPAEDTVRKQAGKVVIFKVTGEP
                     AATPEQINATVIPASGKVIVPLTVWLARKTDLAERMAKGEIGVWMDSFELLEDLVASV
                     ADINSLPLIAINFPRFVDGRGFSLATQLRIRHQYRNELRAVGDVLRDQLYFMKRCGFD
                     AYLIRADRSAEDALASLRDFSDPYQGAFDNPLPVWRRYPRIAHHAAAESRVFVRHEDE
                     VD"
     gene            complement(4739..6400)
                     /locus_tag="PKW44_03810"
     CDS             complement(4739..6400)
                     /locus_tag="PKW44_03810"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HQR50744.1"
                     /translation="MYIYDDIDQRIVDERVAQYRNQTRRYLAGELTDDEYRPLRLQNG
                     LYIQRHAPMMRIAVPYGLLSSRQMRKLADISRRYDKGYGHFSTRQNFQLNWPKLETVP
                     DILAELATVQMHAIQTSGSCVRAITTDQFAGVAPDEIVDPRPFIEIMRQWSSFHPEFA
                     GLPRKFKLAVNGAKHDRALVQAHDIGLELYLDSAGKLAVRVWVGGGLGRTPILGTVIR
                     EHLEWQHALTYCEAILRVYNLYGRRDNAFKARIKILVKALGAEEFSRRVEEEWALHKD
                     GPSTITQAEYDRVASFFAPGRHDALPDQDAAFYAARASNPAFAAWTKRNVHAHKVPGY
                     RAVTLSLKKTGKAPGDASAGQLEVMAELAERYSFGELRVSHEQNIILADVKLSDLFAV
                     WDTARVHELATPNIGLLTDIICCPGGDFCSLANAKSIPIAQAIQEKFDDLDYLHDIGD
                     IELNISGCMNSCGHQHVGHIGVLGVDKDGSEWYQITIGGKQGSDASLGTVIGPSFPAE
                     DVPGVIERLIATYIEERTEEERFIDTVRRIGVAPFKANVYAKEAA"
     gene            complement(6409..7191)
                     /locus_tag="PKW44_03815"
     CDS             complement(6409..7191)
                     /locus_tag="PKW44_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HQR50745.1"
                     /translation="MDFGYIISGFLVGLLVGVTGVGGGSLMTPLLVFLFGFKPAVAVG
                     TDLLFAAITKSGGVLVHHSNHKSVEWRIVSLLAAGSLPASIATLFVLNHYARVGKDIT
                     HAITFSLGIALILTALALFFRNHLQKAGKVSESHEPSRFHHLQAPATVVVGLILGILV
                     TLSSVGAGALGTVALLFLYPRLSTVKIVGTDLAHAIPLTAIGGFGHWQMGNVDFTLLG
                     SLLMGSLPGIWIGSHLSARIPEKVLRPLLASVLMLIGFKFVL"
     gene            7306..8232
                     /locus_tag="PKW44_03820"
     CDS             7306..8232
                     /locus_tag="PKW44_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CysB family HTH-type transcriptional regulator"
                     /protein_id="HQR50746.1"
                     /translation="MKLHQMRYIREVAWQGMNVSVAAEALHTSQPGVSKQIQLLEEEL
                     GLQIFHRSGKRLTGVTEPGRIILDFAERMLREVDNISRVSDDFTHVDAGSLSIATTHT
                     QARYRLPPAVKAFIELYPDVQLNIHQGNPTQVAELVLSGEADIGIATESITMHEELVA
                     LPCYVWNRCVVVPSGHPLQKAGPLTLEKIAAYPIVTYDLGFTGSTSVKRVFEEAGLQP
                     SIVLTAIDSDVIKTYVELGLGIGLLAKMAYNAERDKGLEALDVSHLFPDSTTYLGVRR
                     DVYLRGYVYEFIRLIAPQLDRKSVDSAIKSSA"
     gene            complement(8229..9104)
                     /locus_tag="PKW44_03825"
     CDS             complement(8229..9104)
                     /locus_tag="PKW44_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HQR50747.1"
                     /translation="MSLNIQQGSVDAYTPSEMAYRVKTVGVAKANMGIRQMVGLGIMA
                     GAFISLGAMNYTAAMAQDMPRLVASFMFCLGLVLVIVGSAELFTGNNLIAMAWAQGKV
                     GTRAVLRNWGWVYLANLLGCLATVVLAYLSGILHSVDLGFGVQALKIAVAKTNIPFTE
                     AFFKGILCNALVCLAVWLCFAARTVVDRIFCILFPISAFVNMGFEHSIANMFFIPMGF
                     LAAADPAVLEAAKLAPEAMAHLNLAGFLNNIVAVTLGNIVGGTVLVAGMYFLVYLYGQ
                     HQPHETGRTPTRTKD"
     gene            9185..9679
                     /locus_tag="PKW44_03830"
     CDS             9185..9679
                     /locus_tag="PKW44_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HQR50748.1"
                     /translation="MKKLLILAGALWLGLLPASADSLPPVNPDKALYIVKVEPGVSYD
                     DVVTSLKSAAEGKNFVSPATFPIGEHIRQRGLPLQGVLEVHTYCSLGIGAEILLEYPE
                     FAAFAPCRIAIYEKQGNLYLALDRPTYALRHVDNVNQRARDAAQQMEDTLIWMLDKAR
                     KGEI"
     gene            complement(9693..10145)
                     /locus_tag="PKW44_03835"
     CDS             complement(9693..10145)
                     /locus_tag="PKW44_03835"
                     /inference="COORDINATES: protein motif:HMM:NF015669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HQR50749.1"
                     /translation="MRVRKSRLFSVIALACFVLLTACAARQPSASAEPSRPLAGVEWK
                     LVQVGDAPASGSSPDERAPFVQFDEQGQANGYSAVNNFSGTYVSDGSGLKFGPMIMTR
                     RAGPPAAMALESSLVNALGNTRGYRIAGNTLELLDAGNKPLARFEAAR"
     gene            complement(10227..>10559)
                     /locus_tag="PKW44_03840"
     CDS             complement(10227..>10559)
                     /locus_tag="PKW44_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020182004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HQR50750.1"
                     /translation="ADIIQQDVTSEQGMVMDFSDVKAIARREVVDKWDHAFLVYRGDG
                     TVREFLESLSGHKTVVMDSVPTAENMAAEAFRILDASYRDTYGNHLQLERVRLYETPN
                     SWADALRK"
BASE COUNT         2038 a         2986 c         3262 g         2273 t
ORIGIN      
        1 tgatggcaac tgtgctgatg ttgttcggac tggcgccggc ggttgccccg gtactaggcg
       61 gcgttcttct gggtatacac tggcaggcga ttttcatctt tctggcattg tatgccggac
      121 tgacgctatg ggccgcgatg cgctatctgc cggaaaccat gccgcccgag aaacgcttgc
      181 cgctttccgt gcgccaggtg ctgaaaagct accgcacgat tttcagcgac tgggagtttg
      241 gtcgtctggc gatcgccgtc ggtgcaaatt tctctgggtt ttttctgtat gtgctggcta
      301 gtccggtgtt cctggtcaag caccttgggt tgaatccgca ccagttcggc tggatgttca
      361 tccccaccgt atgcggcatg gtattcggtt cctatctcgc caaacgtgct gcaggacgct
      421 actcgcaaca gagcgtggtg cgtgtcgctt acgcctggat ggcggcaatg gtgctgctca
      481 atctggcgat atgcttcagc ctgcctgcca acccgcttta caatattgcc ccagtggcac
      541 tattcaacat cggcatggca tttgccatgc ccgtgttatc catcgcggca ctggatcgcc
      601 accctggaat tcgcggcaca gccgcctcgg gccaggcttt cgtgcagatg ttcctatcga
      661 cggtttcagc cggcctgatc gtgccgctgg tgtggcaaca tccttcaggc ctggcgctag
      721 caatggttgg ctatttgctg ctgggctggg gggtaatacg cagcagcaag ttgtggcttg
      781 ggaaataaaa aaggaggcgc aggcctcctt tttattgggt ggcaatacta gtttattcga
      841 tcagcagacc gcgcttgccg gtgacgccgt tgtactgctc cgcgtccggc agagcatctt
      901 tcttggccgt gatggccggc catacttttg ccaggcgggc gttgagttca atgtactcct
      961 gctggtcagc gggcacgtca tcttcggcaa aaatggcttc caccgggcat tcggccacac
     1021 acagggtgca gtcgatgcac tcgtcggggt cgatggcgag gaaattcggg ccttccacga
     1081 agcagtccac cgggcagacg tccacgcagt cggtgtattt gcactggatg cagttttcag
     1141 ttacgacgta ggtcattgct ttaactccaa gttctcgatg gggcggattt taccacttat
     1201 tgaatcattc ccgcacccac cgtcatgttg gtgaaatcgt cgatgacgat aaaaccgccg
     1261 gtggcgcggt tgtccgcata agcatcgcag gcgatgggct tcagcagttt gatgccgact
     1321 ttgccgatct cgttcatctt gagctctggc ggtgcgggga tgtcctctag cgtattgatg
     1381 tctactcgct gctccagcgc cgtcaccacg gccttgacag tgctggtggc gtgcttgatg
     1441 aggtatttct tgcccgcctg cagcggggtt tcgtgcatcc agcacagcat ggcgctgagt
     1501 tccttttcca cgcgcggctg gttatcacta tggacgatca tatcgccacg tgaaatatcg
     1561 cgctcgtctt ccagtgtcag cgtgaccgac atcggcgcgt aagcctccgt cagttcgccc
     1621 tcccaggtga cgatgctctt gattttcgaa gtcagcccgg aaggcaatac ggtgacggca
     1681 tcgcctacct tgaccgaacc ggattcgatg cgccccatgt agccgcggaa gtcatgcaac
     1741 tcctcggttt gcgggcggga aacgtactgt accggaaagc ggaaatcctc ggcgttgagc
     1801 ttgccttcga ccgatgcgga ttccaatttt tccagtagcg tctggccttc gtaccacggc
     1861 atgtggtcac cgcgcgtcac caccatgtcg ccttccagtg cggaaatggg gatgaaagtc
     1921 acttcgccgc cgatgaatcc aagcttgcca tagaagtcgg aaaaatcctg gcgaatttgt
     1981 tcgtaaatct cctgcgagta atccaccagg tccatcttgt tgatggcgac cacgatgtgg
     2041 ggaataccca gcaggcgcga aatataggca tggcgacggc tttgcgtcag cacgcccttg
     2101 cgcgcgtcga tcaggataat ggccaggtgc gcagaggagg caccggtgac catgttgcgg
     2161 gtgtactgct cgtgtccggg cgtgtcggcg atgatgaact tgcgcttggg cgtggcgaag
     2221 tagcggtagg ccacgtcgat ggtgatgccc tgttcgcgct cggcctgcag gccgtcggtc
     2281 agcagcgcca ggtgtgcgcc cttggcgcct ttcttgtctt ccacgttctt ggcggcgtcg
     2341 agctggtctt cgaacacacc cttggtgtcc accagcaggc gcccgatcag ggtggatttg
     2401 ccgtcgtcca cgctgccggc ggtggtaaag cgcagcaggg cggtatcagc agcttcaaac
     2461 aggtttttgt ctatggcatt catcagaagt agccttcctt cttcttgtct tccatggtgt
     2521 tgctgccatg gtcgatgatg cgggtttcgc gctcggaacg ggtggcaacg gctgattcgg
     2581 cgacgatttg ctcgatggtg tcggccgagg aatacaccgc accggtgcag ggcgaacagc
     2641 ccagggtgcg aaagcggcac atcacgtttt ccggcacttc gccgggcttg aggttgccgg
     2701 cgtcgtcggc ggggatcagc atggcgccgc gcttcaccat cgggcgctct ttcgcgtagt
     2761 aaagcggcac gatggggatg ttttccagct tgatgtactg ccagatgtcc atttcggtcc
     2821 agttggagat ggggaacacg cggatggatt cgccttcgtg gacgcggcag ttatagatgt
     2881 cccacaactc tgggcgctgg ttcttcgggt cccattggcc gaattcgtcg cggaaggaga
     2941 agatgcgttc cttggcgcgc gagcgttcct cgtcgcggcg agcaccgccc atagccgcgt
     3001 cgtagccgcc ttcttccagc gcgtccagca gcgccttggt cttgagcgcg ccgcagcagc
     3061 gagccacgcc caatttgaga gggtgtgcgc cctgggcgat ggcttcctcg ttgcgatgca
     3121 cgatcagcct ggcgccgatt tccttggcgt accagtcgcg aaactcgtac atttccttga
     3181 atttgtaacc ggtgtccacg tgcagcaggg cgaatggcag cgggccgggg tggaaggcct
     3241 tgcgcgccag gtgcagcatg acgctggaat ccttgccgac ggagtagagc atgaccgggt
     3301 tgcggaattc ggccaccact tcacgcatga tatggatgga ttccgcttcc agggtttgca
     3361 gatgggtgag tgttttgtta tccatgatca gattttcttt acgtgcaggc cgcattcctt
     3421 gccggcttcg ttttcccacc accagcgacc ggcgcgaaga tcctcgccga cggcaatggc
     3481 ccgggtgcag ggcgcacagc cgatactggg gtagtgcttg tcgtgcaacg cattatacgg
     3541 gatggcattg gaacggatat attcccatac ctccttttcc gtccagtccg ccagcggatt
     3601 gaatttggcg aggccgtggt cggcatcgaa ttcctgcact ggcatgtcgg cgcgggtgac
     3661 ggcttgctgg cggcgcatgc cggtgatcca ggccttgttg ccagccagcg cgtgcttgag
     3721 cggttcgacc ttgcgaatcg cgcagcaggc cttgcgcagt tccacactgt ggtaaaaggc
     3781 attgatgccg ttggcttgca catactgctg gagcggctcc ggttgcgggt agagcacccg
     3841 gatgaggact ttttcgccat aggtttcctg caccttggcc aacaggtcgt aggtttccgg
     3901 gttgaggcgg ccggtatcga gcgaaaagat gctgatgccc ggtgcatgtt tggcgatcag
     3961 gtcggtcaac accatatctt cagcgcccag gctgctggcc agcgtggccg ggctgaattc
     4021 gtcctggatg cgctccagca gtatggcggc ggcggcgatt ttggcttgca gggtcatcaa
     4081 tccacctcgt cctcgtgccg gacgaatacc cggctctccg ctgctgcgtg gtgcgctatg
     4141 cgggggtatc gacgccaaac cggcaacggg ttgtcgaacg caccttgata gggatcggaa
     4201 aagtcgcgca ggctggccag ggcatcttcg gcggagcggt cggcacggat cagataggcg
     4261 tcgaaaccgc agcgcttcat gaagtagagc tggtcccgca gcacgtcgcc aaccgcgcgc
     4321 aactcgttgc gatactggtg ccggatgcgc agctgcgttg ccagcgagaa gcctcgtccg
     4381 tcgacaaagc gcggaaagtt gatggcgatc agcggcaggc tgttgatgtc ggcaacgctt
     4441 gcgaccaggt cttccagcaa ttcgaagctg tccatccaga cgccgatctc gcccttggcc
     4501 atgcgttcag ccaagtcagt cttgcgcgcc agccagacgg tcagtggcac gatgaccttg
     4561 cccgaagcag gaatcacggt ggcattgatc tgttcagggg tcgccgccgg ttcgccggtc
     4621 accttgaaaa ttaccacctt gccggcctgc ttgcgcacgg tatcttcagc gggagcgggc
     4681 agggcgacga gcgtccaatc attggcgacg atggcattgt ttttgatgag gttggacatt
     4741 acgcagcctc cttggcgtaa acgttcgcct tgaacggcgc cacgccgatg cggcgcacgg
     4801 tgtcgataaa gcgttcttcc tcggtgcgtt cctcgatgta ggttgcgatc agtctctcga
     4861 tcacacccgg tacgtcttct gccgggaagg aggggccgat caccgtgccg aggctggcat
     4921 cactgccctg tttgccgcca atggtgatct ggtaccactc ggagccgtcc ttgtctacgc
     4981 ccagcacgcc aatgtggccg acgtgctggt gaccgcagga attcatgcag ccggagatgt
     5041 tgagttcgat gtcgccgatg tcgtgcaggt aatcgaggtc gtcgaatttc tcctggatgg
     5101 cctgggcgat cgggatggat ttcgcgttgg ccaggctgca aaaatctccg ccggggcagc
     5161 aaatgatatc ggtcagcagg ccgatattcg gcgtggccag ttcgtgcacg cgggcggtat
     5221 cccatacagc gaacagatca ctcagcttca cgtcggcgag gatgatattc tgctcgtgcg
     5281 acacgcgcaa ttcgccaaac gaatagcgtt cagccagttc cgccatgact tcaagttggc
     5341 ccgcgctggc atcgccgggg gctttgccgg tttttttcag ggaaagcgtc acggcacggt
     5401 aaccgggtac cttgtgcgca tgcacgttgc gcttggtcca ggcggcaaac gccggattgg
     5461 aggcgcgcgc cgcgtagaag gctgcatcct ggtcaggcag ggcgtcgtga cggcctggtg
     5521 cgaaaaagct cgccacgcgg tcatattcgg cttgggtgat ggtgctgggt ccatccttgt
     5581 gcagcgccca ctcctcctcg acccggcggg agaattcctc cgcgccaagc gcctttacca
     5641 ggatcttgat gcgcgccttg aaggcattgt cgcggcgacc gtaaaggttg tagacgcgca
     5701 ggatggcttc acagtaggtc agcgcgtgct gccattccag gtgttcgcgg atgacggtgc
     5761 ccagaatcgg ggtccggccg agcccgccgc ccacccagac gcgcaccgcc agcttgccgg
     5821 cactgtcgag atagagctcc agcccgatat cgtgtgcctg cacaagcgcg cggtcatgct
     5881 tggcaccatt caccgccagc ttgaatttgc gcggcaagcc ggcgaattcc ggatggaagc
     5941 tgctccactg gcgcatgatt tcgatgaaag gccgcggatc gacgatctca tccggcgcga
     6001 cgcccgcgaa ctggtcagtg gtgatcgcac ggacgcagct gccggaagtc tggatcgcgt
     6061 gcatttgcac cgtggcgagt tcggccagga tgtccggtac ggtttccagt ttgggccagt
     6121 tgagctgaaa attctggcgc gtgctgaagt gaccgtaacc cttgtcgtaa cggcgcgaga
     6181 tgtccgccag tttgcgcatt tgccgcgaag acagcaggcc ataaggcacg gcgatgcgca
     6241 tcatgggcgc atggcgctgg atgtagaggc cgttctgcag gcgcagcggg cgatattcgt
     6301 cgtcggtcag ttcgcctgcc aagtagcggc gcgtctggtt gcggtactgg gcgacgcgtt
     6361 cgtcaacgat gcgctgatca atgtcgtcgt agatatacat cacaaatcct ataaaacaaa
     6421 tttaaacccg atcagcatca gcaccgaagc cagaagcggc cgtagcactt tttccgggat
     6481 tctggcgctc aagtggctgc ctatccagat accagggagc gaacccatca gcaggctgcc
     6541 aagcagggtg aaatcgacat tgcccatctg ccagtggccg aagccaccga tggcggtgag
     6601 cggaatggca tgggcgagat cggtgccgac gattttcacg gtagagagtc gcggatagag
     6661 aaatagcagc gctactgtgc ccagggcgcc agcgccgacc gaggacaggg tgaccagaat
     6721 gccgagtatc aggccgacca cgaccgtggc aggggcttgc aggtgatgga agcggctcgg
     6781 ttcatgcgac tcgctcacct tgccggcctt ctgcaagtgg ttacggaaaa ataacgccag
     6841 cgcggtcagg atcaaggcga tgccgagcga gaaagttatt gcgtgggtaa tatccttgcc
     6901 gaccctggcg tagtggttga gcacgaacag ggtggcgatg gatgccggca ggcttccggc
     6961 cgcaagcagg ctgacaatgc gccattcgac cgatttgtgg ttgctgtggt ggacgagtac
     7021 gccgccgctc ttggtgatgg cggcgaacag caggtccgtg ccgactgcta ctgctggctt
     7081 gaagccaaac aggaacacca gtagcggtgt catcagagag ccgccgccga cgcctgttac
     7141 gccgaccagg agtccgacca gaaagccgga aatgatataa ccgaaatcca tttaagaagt
     7201 ttgtgctgga tagagggcgc aactatacca gtgggtactt tccagataaa agaatatgtt
     7261 attctaaatc attctgttta attattaggt gggtagaaag acgttatgaa gttgcaccag
     7321 atgcgttaca tccgcgaagt agcctggcag ggcatgaatg tgtcggtggc ggccgaagcc
     7381 ctgcacactt ctcagcccgg ggtcagcaag cagatccagt tgctggagga ggagctcggg
     7441 ctgcagattt ttcaccgtag cggcaagcgg ctgaccggtg ttaccgagcc gggcaggatc
     7501 attctggatt tcgcggagcg catgctgcgc gaggtggata acatcagccg ggtcagcgac
     7561 gatttcaccc atgtcgatgc tggcagcctc agcatcgcca ccacccacac ccaggcacgc
     7621 tatcgcctgc cgcctgcggt caaggccttc atcgagcttt atcctgacgt gcagctcaat
     7681 atccaccaag gcaatcccac ccaggttgca gagctggtat tgagcgggga ggccgatatc
     7741 ggcatcgcca ccgaatccat taccatgcac gaggagttgg tagcgctgcc gtgctatgta
     7801 tggaaccgct gtgtggtggt gccgtccggc caccctttgc aaaaagctgg accactgacg
     7861 ctggaaaaaa ttgccgctta tccgatcgtc acttacgact tgggttttac cgggagcacc
     7921 tcggtcaagc gggtgttcga ggaggcaggg ctgcaaccga gcattgtgct gactgccatc
     7981 gactccgacg tgatcaagac ctatgtggaa ttgggcctcg gcattggcct gctggccaag
     8041 atggcctaca acgccgagcg cgacaagggg ctggaggcgc tggatgtctc ccatctgttt
     8101 ccggatagca ccacctatct cggcgtgcgt cgggatgtct acctgcgcgg ctatgtctat
     8161 gagttcatcc gcctgatcgc gccgcagctc gaccgcaaat cggtggattc tgcgatcaag
     8221 tcgtcagctt agtctttcgt tcgcgtcggc gttcttccag tctcatgcgg ttggtgctga
     8281 ccgtatagat agaccaggaa atacatgccc gccaccagta ccgtgccgcc gacaatgttg
     8341 cccagggtga cagccacgat attgttgaga aaacctgcca ggttgagatg cgccatcgcc
     8401 tccggcgcca gctttgcagc ttccagcacg gccggatcgg cggcagccag aaaccccatg
     8461 gggataaaga acatgttggc gatgctatgc tcgaagccca tgttgacgaa ggccgagatg
     8521 gggaaaagga tgcagaaaat gcggtcgact acggtgcgcg cggcgaagca tagccagact
     8581 gccaggcaca ccagcgcatt gcaaaggatg cctttgaaaa aagcctcggt aaatggaatg
     8641 ttggttttgg cgacggcgat cttgagtgct tggacgccaa aaccaaggtc aaccgaatgt
     8701 aggatgccgg agagataggc cagtaccacg gtcgctaggc agcccagcag attggcgagg
     8761 tatacccacc cccagttgcg cagcaccgcg cgggtgccga ccttgccctg cgcccaggcc
     8821 atggcgatca ggttattgcc ggtgaatagt tcggcactgc cgacgatcac cagtaccaga
     8881 ccgaggcaga acatgaagga agcgaccagg cgcggcatgt cctgcgccat cgcggcggta
     8941 tagttcatgg cgcccagtga aatgaaggcg ccagccatga tcccgagccc gaccatctgg
     9001 cggatgccca tgttagcctt ggcgaccccg acggttttga cgcgataggc catctccgaa
     9061 ggcgtgtagg catccacgga accttgctga atattgagtg acatactgtc ggtgtccttg
     9121 cgctgggtga aatggcgcgt attgtatgcg aattcgcctt gatcaagaat tgaaggaatg
     9181 caaaatgaaa aagctgctga tactggctgg ggcgctgtgg ctgggtttat tgcctgccag
     9241 cgccgattcc ttaccaccgg tcaatcctga caaggccttg tacatcgtca aggtagaacc
     9301 cggcgtcagc tatgacgatg tcgtgaccag cctgaaatcg gcggccgaag gcaagaattt
     9361 cgtcagcccg gcgactttcc ccatcggcga acatatcagg cagcgcggcc tgccgctgca
     9421 aggcgttctg gaagtgcaca cctattgcag tttgggcatc ggcgcggaaa ttttgctgga
     9481 atatcccgaa ttcgccgctt tcgccccttg ccgcatcgcc atttacgaaa agcagggcaa
     9541 cttgtacctg gcgctggatc ggcccaccta cgcgctacgc catgtggata atgtcaacca
     9601 gcgcgcccgg gatgccgcgc aacagatgga agacaccttg atctggatgc tggacaaggc
     9661 acgtaaagga gaaatttagc gggcggattg tttcaacggg ctgcctcgaa gcgtgccagc
     9721 ggcttgttgc cggcgtcgag cagttccagc gtgttgccgg caatgcgata gccgcgggta
     9781 ttccccaaag cgtttaccag tgaagattca agtgccatgg cggcaggcgg cccagcacgg
     9841 cgggtcatga tcatgggacc gaatttcagg ccggaaccat cactgacata ggtgccgctg
     9901 aaattgttga ccgcactgta accgttggcc tggccttgtt cgtcgaactg gacgaagggc
     9961 gctcgctcgt ccggagaact gccggacgcc ggcgcatcgc cgacctggac cagtttccat
    10021 tcgaccccgg ccagcgggcg tgacggctcg gccgatgccg atggctgtct ggccgcgcag
    10081 gcggtgagca gcacgaagca cgccagggcg atgactgaaa aaagtctgga tttgcgaacc
    10141 cgcattgaag tcctcctgat gagccaaata ttggattcca cccctcaagt ggcggcgata
    10201 gtctgtcgga tcgggatgaa gccgtgctat ttgcgtagcg cgtccgccca gctattgggc
    10261 gtctcgtaca gcctgacccg ttccagttgc aggtggttgc cataagtgtc acggtagctg
    10321 gcgtcaagga tgcggaacgc ctccgccgcc atgttttccg ccgtcggcac gctatccatc
    10381 acaacggttt tgtgccccga caacgactcc agaaattcgc gcacagtgcc gtcgccgcga
    10441 tagaccagga aggcgtgatc ccacttgtcc accacctcgc gccgggcaat ggctttgacg
    10501 tcggaaaaat ccatcaccat accctgctcg gaagttacgt cctgctggat gatgtcagc
//
LOCUS       DAOMFA010000049        10421 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_677_length_10421_cov_5.371021, whole genome shotgun sequence.
ACCESSION   DAOMFA010000049 DAOMFA010000000
VERSION     DAOMFA010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 10421)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10421)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10421
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_677_length_10421_cov_5.371021"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(100..552)
                     /locus_tag="PKW44_03845"
     CDS             complement(100..552)
                     /locus_tag="PKW44_03845"
                     /inference="COORDINATES: protein motif:HMM:NF036878.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HQR50751.1"
                     /translation="MQTHYVFVDFDNTHLGNIGLLSKSTSKLRIKIFLGSHYVSIPLA
                     LARALQPLGKDAEYIQLDHARHNAIDFNIAFQLGELASQHPDAQFSILSNDPAFNEII
                     SGLRAKGVDCTRFSDMESLFKHLSIPTPAVKEEPKSDKVVAIKEPWQR"
     gene            complement(647..1447)
                     /locus_tag="PKW44_03850"
     CDS             complement(647..1447)
                     /locus_tag="PKW44_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01458 family HAD-type hydrolase"
                     /protein_id="HQR50752.1"
                     /translation="MNPSPSVKGILFDLDGVLYVGSRVIEGAIEAVAHIRASGIACRF
                     VTNTSTLSLASLQNKINVLGFSITKEEIVSAPQAARLYLKRQPDPVCRFLMADNVKQD
                     FTDFRQSDTEANYIVVGDIGTTWSYTLLNEVFHCLMNGAQLIAIHKNRFWQTETGLQM
                     DIGGFIEALEYASGSKAMVIGKPATDFFRIALDDMGLQPAEAAIVGDDIDADVGGGQQ
                     AGLRGILVKTGKYRQSYAEASRIKPDLVIDSIRDLPSALTGLSTTAPV"
     gene            complement(1648..2946)
                     /locus_tag="PKW44_03855"
     CDS             complement(1648..2946)
                     /locus_tag="PKW44_03855"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQR50753.1"
                     /translation="MLLDFNGALLFITHDRRFLDRLATRIIELDRGHITEFKGNYSAY
                     QIKKAELLEVEATHAAKFDKVLAQEEAWIRQGIKARRTRNEGRVRRLERLRLERTARR
                     ERQGNVKLALDEGERSGKLVAELSHVSKSFGDKVVIRDFSTRILRGDRIGLLGPNGAG
                     KSTLLKLILGELEPDSGEVKRGTSLNLAYFDQMREQLDEEATLADTISPGSDYVQIGS
                     ERKHVISYLEDFLFPPQRARAPVKSLSGGERNRLLLARLFARPANVLVLDEPTNDLDI
                     DTLELLEELLQDFGGTLFLVSHDRAFLENTVTQVIAFEGNGKLEEFGGGYDDWRRFTD
                     QQAAQAAARPKAELTPKPATVKPTQAKLSYKEQRELEALPDKIEALETEQGDINATLS
                     NGDLYRSDPQRVKALQARLTVIEQEIELALARWEALERRE"
     gene            complement(2974..3543)
                     /locus_tag="PKW44_03860"
                     /pseudo
     CDS             complement(2974..3543)
                     /locus_tag="PKW44_03860"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            complement(3665..4522)
                     /locus_tag="PKW44_03865"
     CDS             complement(3665..4522)
                     /locus_tag="PKW44_03865"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HQR50754.1"
                     /translation="MLSIKTRVVLAGAASLLILNACNSKQNTAAPASKDETVATVNGT
                     AISKSRIDMIVKQGAAGHGDSPEARKAITEQLVMQTLVAEEAVKKGLDKSPEVLEQLD
                     VLKQSVLANAYVQDYIKNHKVSDGDLKAEYDRIKAGISGTEYKARHILVATEAEANDI
                     IAKLKKDPGAFEQLAMEKSLDTGSKARGGDLGWFDLNKMVPEFGDAVAKLEKGKISET
                     PVKTQYGYHVILLEDSKPIEAPPFEEVKSGLAEQLQQKNLKKELDDLKAKAKIEIAGA
                     PAAASAPTK"
     gene            complement(4614..5066)
                     /locus_tag="PKW44_03870"
     CDS             complement(4614..5066)
                     /locus_tag="PKW44_03870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR50755.1"
                     /translation="MRNFATIRSLALLFFAALFSAQSSAYDLSSLKSLYSMVGGDKAV
                     AQISNDMLVTAASDSRLKPLLGNTDVKTLAPKVTEQICAILGGDCKAPLNSQQVADGA
                     KKLDASQTSALTEAFNSALSKSGTNSATQGLVSKAVGPQLGGIIGGLL"
     gene            complement(5156..7141)
                     /locus_tag="PKW44_03875"
     CDS             complement(5156..7141)
                     /locus_tag="PKW44_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HQR50756.1"
                     /translation="MLSKLNPPQREAVKYLDGPLLVLAGAGSGKTRVITHKIVHLIDS
                     CGYAPREIAAITFTNKAAREMQERVGALLQGKPSKGLTVTTFHSLGLQMLRQEAQLLG
                     YKPQFSILDSSDSFKILADVLATTDKQLLRRTQWQISGWKNAFLTPDQAKAQAEEEVA
                     HVAARVYQLYQQTLKAYQAVDFDDLIRLPVELFQQHPQALEKWQRKLKYLLIDEYHDT
                     NACQYKLVKLLTGVEGRFTAVGDDDQAIYGWRGADVENLRQLTEDFPRLKVIKLEQNY
                     RSTVRILRAANQVIANNPKLFDKKLWSEHGTGDPIQISAMRDEEHEADTVVMKLLAYK
                     FEHRSKFADYAILYRGNHQSRLLEEKLREHKVPYTVSGGQSFFDKAEIKDLVSYLRLI
                     ANEDDDPAFIRAATTPKKGIGNTTLERLGEYAAGKHISLFEAAFEGDFQQHIGHKQLD
                     DLLTFCRFIQRMQERAVKDPAGEVLNDLLAAIQYEMFLYDSEEPRAAESKWNNVMDFI
                     GWLTRKSEADESTLLQLTQTVALMSMLEGREEEVDAVKLSTLHAAKGLEFGHVFLIGV
                     EEGILPHRESVDNGKIEEERRLMYVGITRAERSLHISWCRKRRRAGETTACEPSRFIA
                     ELPADDVRHSGQPGADPAAAKENGRAAMASIRAMLGK"
     gene            7305..8102
                     /locus_tag="PKW44_03880"
     CDS             7305..8102
                     /locus_tag="PKW44_03880"
                     /inference="COORDINATES: protein motif:HMM:NF013625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HQR50757.1"
                     /translation="MATLKEGDSGKKVIKLQKRLQALGFSPGNIDGEFGPATRAAVIA
                     FQKSEGLLADGIAGPRTQTALGMADDDTLPSAIHEVTVSLVSCMFPLTPVGNIKQHLP
                     PVLDALLEATLAEKPMVLMALATIRAETEGFEPISEGRSKYNTSPNGYPFDLYDNRRD
                     LGNQGSGDGEKFRGRGFIQLTGRANYQTYGAAIGLGKQLVNKPELANDPVIASRLLAS
                     FLKAKEREIKEVLLENDLRQARKLVNGGSHGLDRFSEAYRIGEQLLA"
     gene            8118..9197
                     /gene="gcvT"
                     /locus_tag="PKW44_03885"
     CDS             8118..9197
                     /gene="gcvT"
                     /locus_tag="PKW44_03885"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018076872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HQR50758.1"
                     /translation="MTRRTVLYDAHLQAGARMVDFGGWEMPLHYGSQIEEHHAVRRDC
                     GMFDVSHMCAVDIIGPGARPFLQRLLANDVARLAQPGKALYSAMLNPSGGVIDDLIVY
                     FFDSAHYRIVVNAAAADKDLAWMAARLAEWGMDMTITPRRELGMIAVQGPSARERAWQ
                     ALPQCRAASAELQPFFAVPVDDLLVARTGYTGEDGFEITLPTQYAPALWQALLEAGVR
                     PCGLGARDTLRLEAGMNLYGQDMDESVSPLDAGMGWSVNLDGTRDFVGKTALIENGQH
                     SRLLGLVLLERGVLRTQQNVITAHGAGIITSGTFSPSLQQAIALARLPMAVATGETVE
                     VEIRDRRHAARVVRPPFVRHGKILV"
     gene            9218..9598
                     /gene="gcvH"
                     /locus_tag="PKW44_03890"
     CDS             9218..9598
                     /gene="gcvH"
                     /locus_tag="PKW44_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019919738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HQR50759.1"
                     /translation="MSIPATLKYTPSHEWVRLEADGSITVGITDHAQALLGDMVFIEL
                     PTVGKSAAAEEQVAVVESVKAASDIYAPVAGVVTEANQNLADAPERVNEDAYAAWLFK
                     MQPASVADVDGLLDAAGYASEIGA"
     gene            9608..>10421
                     /locus_tag="PKW44_03895"
     CDS             9608..>10421
                     /locus_tag="PKW44_03895"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019916341.1"
                     /note="acts in conjunction with GvcH to form
                     H-protein-S-aminomethyldihydrolipoyllysine from glycine;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine dehydrogenase
                     (aminomethyl-transferring)"
                     /protein_id="HQR50760.1"
                     /translation="MSSAPSLSALEQHDAFLVRHLGSGEADIAAILRAVGASGLDELI
                     AQTIPADIRLTTPPDLPPPRSEHEALAAIKRMAGNNRVMRSLIGMGYADTITPAVIAR
                     SLLQNPGWYTAYTPYQAEIAQGRLEALINYQQMVIDLSGLPLANASLLDEATAAAEAM
                     AMARRISQSPSDTFFVDAHCFPQTVDVLRTRAAFFGFELVFGAPEQAGRQAVFGALLQ
                     YPDDTGEVADLTPHIAAVQAQGGIAAVSADLLSLVLLKSPGAMGADIALGSAQ"
BASE COUNT         1828 a         3097 c         3281 g         2215 t
ORIGIN      
        1 gagtcccccg cctggcgggt tttggtctga tcggcggata tgcgtatttc ctgtacgcag
       61 gactcattca gtagattggc cagtaacctg gcttaaacac tatcgctgcc agggctcctt
      121 gatggcaacg accttatcgg acttgggctc ttccttcacg gcaggagtag gtatggaaag
      181 atgtttgaac aggctttcca tgtctgaaaa cctggtgcag tcaacccctt tggccctcag
      241 gccgctgata atttcattga atgccggatc gttggacagg atggaaaatt gcgcatcggg
      301 atgctggctt gccaactcgc ccagttggaa ggcaatattg aagtcaatgg cattgtgacg
      361 cgcatgatcg agctggatgt attcggcgtc ctttcccagc ggttgcagtg cgcgcgccag
      421 agcaagcggg atcgagacgt aatgggaccc cagaaaaatc ttgatcctga gtttgctggt
      481 acttttgctg agaaggccaa tgttacccag atgagtgttg tcgaaatcga caaagacata
      541 atgcgtttgc atgatgcacc cctccgccat tggcttacgt gcttactata ccaataaatt
      601 ccacgcatgg tcgatacata aaaaactggc agcgataaac cttgttctat acaggcgccg
      661 tggtggaaag gccggtcagt gcgcttggca aatccctgat cgagtcgatg accaggtctg
      721 gtttgatcct ggaggcttcg gcataggatt gacggtattt gccggttttc accaggatgc
      781 cccgcaggcc agcctgctga cctccgccta catcggcatc gatgtcgtcg ccgacgatgg
      841 ctgcctctgc gggctgcaac cccatgtcat ccagcgcgat gcgaaaaaaa tcggtggcag
      901 gcttgccgat gaccatggcc ttgctgccgc tggcatattc cagggcctcg ataaacccgc
      961 cgatatccat ctgcaagccg gtttcggtct gccagaagcg gttcttgtgg atggcgatga
     1021 gttgcgcccc gttcatcagg cagtgaaaca cctcgttcag caaggtgtag gaccaggttg
     1081 ttcctatgtc gccaacgaca atgtagttgg cttcggtatc ggattgacga aaatccgtga
     1141 aatcctgctt cacgttgtcc gccattaaaa atcggcacac cggatcgggc tgccgcttca
     1201 aatagagccg cgccgcttgc ggagcgctga cgatttcctc tttcgttatg gaaaatccca
     1261 gcacattgat tttgttttgc agcgaagcca gtgacagggt gctggtgttt gtgacgaaac
     1321 ggcacgcaat gccgctggcg cggatatgtg ccacagcctc gatcgcccct tcaatgaccc
     1381 gagagccaac ataaagcacg ccgtccagat cgaacaagat gcctttgacg cttggggagg
     1441 gattcatttg cgataagcaa aaaactcagg aatggaaata ataacccaac cagccacctt
     1501 cggtgaccgc tcttatttga ggcggttact ttttgctgag tcaatgactg cctcgggtcg
     1561 gctattgccc tgcaacatag cgaaacaacc tcaatccatt tcccgggtcg agcatatcct
     1621 gatcgccagg gcgtgagctg gaacgggcta ctccctgcgt tccagtgcct cccaacgcgc
     1681 cagcgccagt tctatttcct gttcgatgac ggtcaggcgg gcctgcagcg cctttacccg
     1741 ctgcggatcg ctgcggtaga ggtcgccgtt cgacagggtg gcgttgatgt cgccctgttc
     1801 cgtttccagt gcttctatct tgtccggcag cgcttccagt tcgcgttgtt ccttgtagct
     1861 cagtttggcc tgtgtgggtt tgacggtggc gggcttgggc gtcagttcgg ccttgggtct
     1921 ggccgccgcc tgtgctgctt gctggtcggt aaagcgcctc cagtcgtcgt agccgccgcc
     1981 gaattcttcc agtttgccgt tgccctcgaa ggcaatgacc tgggtcacgg tgttttccag
     2041 aaaggcgcgg tcgtggctga ccaggaacaa ggtgccgccg aagtcctgca gcagttcctc
     2101 cagcagttcc agggtgtcga tatcgaggtc gttggtcggc tcgtccagta ccaggacgtt
     2161 ggcggggcgg gcgaacaggc gcgcgagtaa caatcgattg cgctcgccgc cggacaagga
     2221 cttcaccggc gcccgcgcgc gctgcggtgg gaacagaaaa tcctccagat agctgatgac
     2281 gtgcttgcgc tcgctgccga tctgcacgta gtccgatcct ggggaaattg tgtcggccag
     2341 cgtggcttcc tcgtccaact gctcgcgcat ctggtcgaaa taggcgaggt tgaggctggt
     2401 gccgcgcttg acctcgccgc tgtccggctc cagttcgccc aggatcagtt tcagcagcgt
     2461 ggatttgccg gcaccgttgg ggccgagcag gccgatgcgg tcgccgcgca ggatgcgtgt
     2521 ggaaaagtcg cggatgacga ccttgtcgcc aaaggatttg ctcacatgcg acaactcggc
     2581 caccagtttg ccggagcgtt cgccttcgtc cagcgccagt ttgacgttgc cttggcgctc
     2641 gcgtcgcgcc gtgcgttcga gacgtaatct ttccagccgc cgcacgcggc cctcgttgcg
     2701 ggtgcggcgc gccttgatgc cctggcgtat ccaggcttcc tcctgcgcca gcaccttgtc
     2761 gaacttggcg gcgtgggtag cttcgacctc cagcagttcg gcctttttga tctgataggc
     2821 gctgtaattg cccttgaatt cggtgatgtg gccgcggtcg agttcgatga tgcgcgtcgc
     2881 aagccgatcc agaaagcgcc ggtcgtgggt gataaacagc agcgcgccgt tgaagtccag
     2941 cagcatattc tccagccatt cgatggcggc gaagtcgagg tggttggtcg gctcgtccag
     3001 cagcagcact tccggctctg ccaccagtgc ccgcgccagt gccacccgct tgcgccagcc
     3061 gcccgacagc gtggacaccg gcgtatcttc cgtaagttcg agccgcgaca gcacggtctc
     3121 gatacgtgcc tggacctgcc agccgttttg cgtctccagc tcgtgttgca gcgcctgcat
     3181 tttatcgagc agggcggcag tgtcggcgcc agcctcgccc atggcgtggg acacggcgtg
     3241 gtaatcgatc agcacctgcc gcagcgtacc caggccttcc gctactgcct cgaatacggt
     3301 gtgctccggg tccagtggcg gttcctgcgg cacgtaggcc aggcgcatgc cagacgcgcg
     3361 ccagacgctg ccatcgtcca gtctggtttc cccggccagc actttcagca ggctggattt
     3421 tccggtgccg ttgcgaccga tcagtccgac ccgttcaccg gggtcgagca gcaggctggc
     3481 gtgatcgagc aggggggtga ggccgtaggc gagagaggca ttgtcgagcg tgatcagcgg
     3541 catgggcgat gttccgggat gaggcgcgta ttgtagcgca ggggatgggc ttgccgtctg
     3601 cggcgcgggg cacaaaacaa aaaggcggcc ctcgggccgc ctttttgtgc gggttgagtg
     3661 cgctttactt ggtcggtgca gacgcggctg cgggagctcc ggcaatttcg attttggcct
     3721 tggccttgag gtcgtccagt tcctttttca ggttcttctg ctggagttgc tcggccaggc
     3781 cggatttgac ttcttcgaag ggaggcgcct cgatcggctt ggaatcttcc agcaggatga
     3841 cgtgatagcc gtactgggtt ttgacaggtg tttcactgat cttgcctttc tccagtttgg
     3901 cgaccgcatc gccgaactcc ggcaccatct tgttcaagtc aaaccagccc agatcgcctc
     3961 ctctggcctt ggagccagtg tccagggatt tttccatggc cagctgctcg aaagcaccag
     4021 gatccttctt cagcttggcg atgatgtcgt tggcctcggc ttccgtggcc accaggatgt
     4081 gacgtgcctt gtattcggtc ccgctgatcc cggccttgat cctgtcgtac tcggccttca
     4141 ggtcgccgtc gctgaccttg tgatttttga tgtaatcctg cacataggca ttggccagca
     4201 cggactgctt gagcacgtcg agttgctcca gaacttcagg cgacttgtcc agccccttct
     4261 tgacggcttc ctcggcaacc agcgtctgca tgaccagctg ctcggtgatg gctttcctgg
     4321 cttccgggct gtcgccatgg ccggcggcgc cctgcttgac gatcatgtca atgcggctct
     4381 tactgatagc cgtgccattt acagtagcga cggtttcatc tttcgaagcc ggcgcggcag
     4441 tattttgttt ggaattgcat gcattgagga tcagcaggga agctgcgcca gccagcacga
     4501 ctcgcgtttt gattgacaac atggatggtt ccttaaaaag ttgcggaaat atgatgtgga
     4561 agcctgccag ccagaggcag gcaagggcga tgtccggcaa gtgataacgg agtttaaagc
     4621 agtccgccga tgatcccgcc gagttgcggt ccgacagcct tgctgaccag tccctgcgtc
     4681 gccgagttcg tccccgattt ggacagggcg ctgttgaatg cttcggtcag cgcgctggtt
     4741 tgcgaggcat ccagcttctt cgcgccatcg gcgacctgct gcgagttgag cggggccttg
     4801 cagtcaccgc ccagaatcgc gcaaatttgc tccgtcacct tcggcgccag cgtcttgaca
     4861 tcggtattgc cgagcagcgg cttcaggcgg ctatcgcttg ctgccgtcac cagcatgtcg
     4921 tttgaaatct gcgccacggc tttgtcgccg ccgaccatgc tgtagaggct tttcaaggag
     4981 ctaaggtcgt aagcgctgga ttgcgcactg aacaacgctg caaaaaacag tagtgcgaga
     5041 gaacgaatcg tggcgaagtt gcgcatggta ttggtctcta tcagaggatt gtggttgaag
     5101 gggcttttcc ggacactagt ttaacacgat gaactcgccg aggaatgcgg ggccgctatt
     5161 tcccaagcat ggcccggatg cttgccattg ccgcacgtcc gttttccttc gcggcagcgg
     5221 ggtcagcgcc cggttggcca gaatggcgca cgtcatcggc gggcagctcg gcgatgaagc
     5281 ggcttggttc gcaggctgtg gtttcgccag cgcgcctgcg cttgcggcac caggagatgt
     5341 gcagcgagcg ttcggcgcgg gtgatgccga catacatcag gcggcgctct tcctcgatct
     5401 tgccgttgtc caccgattcc cggtgcggca ggatgccttc ttccacgccg atcaggaaca
     5461 cgtggccgaa ttccagcccc ttggcggcgt gcagggtgga gagcttgacc gcgtcgactt
     5521 cctcctcgcg gccctccagc atgctcatca gggcgacggt ctgggtgagt tgcagcaagg
     5581 tggattcgtc ggcctcgctt ttcctggtca gccagccgat gaagtccatg acgttgttcc
     5641 acttgctctc ggcggcgcgc ggctcctcgc tgtcatagag gaacatctcg tactggatgg
     5701 cggccagcag gtcgttcagc acttcgcccg ccgggtcctt caccgcgcgt tcctgcatgc
     5761 gctggatgaa gcggcagaag gtgagcaggt cgtccagttg cttgtggccg atgtgctgct
     5821 ggaaatcgcc ttcgaaggcg gcctcgaaca gcgaaatgtg cttgccggcg gcgtattcgc
     5881 ccaagcgttc cagtgtggtg ttgccgatgc ctttcttcgg cgtcgtcgct gcgcggataa
     5941 aggccgggtc atcgtcctcg ttggcgatca accgcagata gctcaccaga tccttgattt
     6001 cggccttgtc gaagaacgac tgtccgccag acacggtata aggtaccttg tgttcgcgca
     6061 gtttttcttc cagcaaccgc gactggtggt tgccgcggta gaggatggcg taatcggcga
     6121 acttgctgcg gtgctcgaac ttgtaggcca gcagcttcat caccacggta tcggcctcgt
     6181 gctcctcgtc gcgcatggcg ctgatctgga tcgggtcgcc ggtgccgtgc tcgctccaca
     6241 attttttgtc gaacagcttg gggttgttgg caatcacctg gttggcggcg cgcaggatgc
     6301 gtaccgtcga acggtagttc tgctccagct tgatgacctt gagcctgggg aaatcctcgg
     6361 tcagttggcg caggttttcc acgtcggccc cgcgccagcc atagatggcc tggtcgtcgt
     6421 cgccgaccgc ggtgaaacgg ccttccacgc cggtgagcag ttttacgagc ttatattggc
     6481 aggcgttggt gtcatggtat tcgtcgatca gcaggtactt gagtttgcgc tgccattttt
     6541 ccagcgcctg cggatgctgc tggaacagtt ctaccggcag ccggatcagg tcgtcgaagt
     6601 ccaccgcctg gtaggctttc agcgtctgct ggtagagctg gtagacgcga gcggcgacat
     6661 gggcgacttc ctcttcagcc tgcgccttgg cctggtcggg ggtgaggaag gcgttcttcc
     6721 agccggaaat ctgccactgg gtgcgcctta ataattgctt gtcggtggtc gccagcacgt
     6781 cggccaggat cttgaagctg tcggaggaat cgaggatgga aaactgcggc ttgtagccca
     6841 gcagttgcgc ctcctggcgc agcatctgca agcccagcga gtggaaggtg gtgaccgtca
     6901 gtcccttgct cggcttgcct tgcagcaagg cccccacgcg ctcctgcatt tcgcgcgctg
     6961 ccttgttggt aaaggtgatg gcggcgatct cgcgtggcgc gtagccgcaa ctgtcgatca
     7021 aatgcacgat cttgtgggtg atgacgcgcg tcttgccgct gccggcgccg gccagcacca
     7081 gcaaggggcc gtccaggtat ttgactgctt cgcgctgcgg gggattgagc ttgcttaaca
     7141 ccaggtgaat gagggctgaa accgaaaggt cgccagttta ccggacattg ccgatgcaag
     7201 gcagattccc gccatgccgg ccaaaatttt tctctgcata tcaactgccg gaatcagttt
     7261 gtcctacaat ttggtttcct gttcgaggcg accaaggagg ggccatggca acgttgaagg
     7321 aaggcgattc cggcaagaaa gtcatcaagc tgcaaaagcg tctgcaggcg ctcggcttca
     7381 gcccgggcaa tatcgacggc gagttcggcc ctgccactag ggcggcggtc attgcctttc
     7441 agaagagcga aggcttgctg gccgacggca tcgccgggcc gcgtacccaa accgccttgg
     7501 gcatggccga tgacgatacg ctgccctcgg cgatccatga ggtcacggtc agcctggtgt
     7561 cgtgcatgtt cccgctgacg ccggtcggca acatcaagca gcatctgccg ccggtgctgg
     7621 atgcgctgct ggaggctacc ctggccgaaa aacccatggt gctgatggcg ctggcgacga
     7681 tacgtgctga aaccgagggc ttcgagccga tcagcgaagg gcgctccaag tacaacacct
     7741 cgcccaacgg ctatcctttc gacctctacg ataaccggcg cgacctcggc aaccagggca
     7801 gcggggacgg cgagaaattc aggggccgcg gcttcattca gttgaccggg cgtgccaatt
     7861 accagacgta cggtgccgcc atcggcttgg gcaagcagct ggtgaacaag ccggaactgg
     7921 ccaatgaccc ggtgatcgcg tcgcgcctgc tggcgagctt tctcaaggcc aaggagcgtg
     7981 agatcaagga ggtgctgctg gaaaacgacc tcaggcaggc gcgcaagctg gtcaacggcg
     8041 gcagccacgg tctggaccgc ttcagcgaag cctaccgcat tggcgagcag ttgctggcct
     8101 gagtccgaga gccctgcatg acgcgccgca ccgtcctgta tgacgcgcat ctgcaagcgg
     8161 gtgcgcgcat ggtcgatttc ggcggctggg agatgccgct gcactacggt tcgcaaatcg
     8221 aggagcacca tgcggtgcgg cgtgactgcg gcatgttcga cgtgtcgcac atgtgcgccg
     8281 tggacatcat cgggccgggg gccaggcctt tcttgcagcg cctgctggcc aacgacgtgg
     8341 cccggctcgc gcagcccggg aaggcgcttt acagcgccat gctcaatccg tccggcggcg
     8401 tgatcgatga cctcatcgtg tattttttcg acagcgcgca ctaccgaatc gtcgtcaacg
     8461 ccgccgccgc ggacaaggat ctcgcctgga tggctgcacg cctggcggag tgggggatgg
     8521 acatgacgat caccccacgc cgcgagctgg gcatgatcgc ggtgcagggc cccagcgcgc
     8581 gtgaacgggc gtggcaggcg ttgccgcaat gccgcgcagc cagtgctgag ttgcagccgt
     8641 tctttgccgt gccggtagac gatttgttgg tcgcgcgcac cggctacacc ggcgaggacg
     8701 gtttcgaaat caccttgccg acgcagtacg caccggccct gtggcaggcg ctgctggaag
     8761 ccggggtgcg gccatgcggc ctgggcgcgc gcgacaccct gcgcctggag gccggcatga
     8821 acctgtacgg ccaggacatg gacgaatcgg tgtcgccgct ggatgcgggc atgggctgga
     8881 gcgtgaacct ggacggtacg cgcgatttcg tcggcaagac agccctgatc gagaatggtc
     8941 agcacagccg cctgctggga ttggtgctgc tggagcgtgg cgtgctgcgc acgcagcaga
     9001 acgtcatcac ggcgcatgga gcaggtatca ttaccagcgg cacgttttcg ccgagcctgc
     9061 agcaggcgat tgccctggcg cgcctgccca tggccgtcgc gaccggcgag acggtggaag
     9121 tggaaatccg cgaccggcgg cacgccgcca gggtggtcag accacccttc gtccggcatg
     9181 gaaaaattct ggtttaacga tcgatctgga gatttttatg agcattcccg caaccctgaa
     9241 atacacccct agccacgagt gggtgcgcct tgaggccgac ggcagcatta ccgtgggcat
     9301 taccgaccac gcgcaggcgt tgctgggcga catggtgttc atcgaactgc caaccgtggg
     9361 gaaaagcgct gccgcggagg agcaggtggc ggtggtggag tcggtcaagg cggcatcgga
     9421 tatttacgcc ccggtggccg gcgtggtgac cgaggccaac cagaacctgg ccgatgcgcc
     9481 ggagcgcgta aacgaggacg cctacgctgc ctggctgttc aagatgcagc cggccagcgt
     9541 ggccgatgtc gacggcctgc tggacgccgc cggctatgcc agcgagatcg gcgcctagtc
     9601 gaaattcatg tccagcgcgc cgtccctgtc agcgctcgaa cagcacgacg ccttcctcgt
     9661 gcgccatttg ggttccggtg aggccgacat cgcggccata ctgcgggcgg tcggcgcgtc
     9721 cggcctggac gaactgatcg cgcagaccat cccggccgat atccgtctca ccacccctcc
     9781 tgacctgccg ccgccgcgca gcgaacacga ggcgctggcc gcgatcaagc gcatggccgg
     9841 caacaaccgc gtcatgcgct cactgatcgg tatgggttac gccgatacca tcacccctgc
     9901 cgtcatcgcc cgcagcctgc tgcagaaccc cggctggtac accgcctata cgccctacca
     9961 ggccgagatc gcgcagggac ggctggaggc gctgatcaac taccagcaga tggtgatcga
    10021 cctcagcggc ctgccgctgg ccaacgcctc gctgctggac gaagcgaccg ccgccgccga
    10081 ggccatggcg atggcgcggc gcatcagcca gtcgccttcc gacacctttt tcgtcgacgc
    10141 ccactgcttc ccgcagaccg tggacgtgct gcgcacccgt gccgcttttt tcggcttcga
    10201 gctggtattc ggtgcgccgg agcaggccgg gcgccaagcg gtgtttggtg ccctgctgca
    10261 atacccggac gacaccggtg aagtggccga cctcacgccg cacatcgcgg cggtgcaggc
    10321 gcagggcggc atcgcggcgg tgtctgccga tctattatcg ctggtgctgc tcaagtcgcc
    10381 gggcgccatg ggcgccgata tcgcgctcgg ctctgcgcag c
//
LOCUS       DAOMFA010000050        10403 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_679_length_10403_cov_5.367027, whole genome shotgun sequence.
ACCESSION   DAOMFA010000050 DAOMFA010000000
VERSION     DAOMFA010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 10403)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10403)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10403
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_679_length_10403_cov_5.367027"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..124)
                     /locus_tag="PKW44_03900"
     CDS             complement(<1..124)
                     /locus_tag="PKW44_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle receptor subunit
                     alpha"
                     /protein_id="HQR50761.1"
                     /translation="MFDNLTGRLAGVIKNLRGQARLTEDNIQEALREVRMALLEA"
     gene            167..964
                     /gene="ccsA"
                     /locus_tag="PKW44_03905"
     CDS             167..964
                     /gene="ccsA"
                     /locus_tag="PKW44_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HQR50762.1"
                     /translation="MLDFLPYIAAALLYTLVAFLQWHTKKFASGWYERVAVATGLALH
                     GWLLAHDMFTGNGVNLGLINALSAIFWLTVLIYWLASLKYNLHALQAFVLPPATLFVL
                     LQKLVPESHVLPYTGDALFGAHLVIALLAYSLFTFAALHAGLMAIAERSLHNKTPLFR
                     LPDFPPIMPMERLLFQIIGIGFVLLTLTLVSGIFFTEQLFHQALQFNHKTVFSIAAWL
                     IFGALLVGRRVQGWRGRVAVRWTLGGFVLLLLAYVGSKFVLEVLLGR"
     gene            complement(975..1640)
                     /locus_tag="PKW44_03910"
     CDS             complement(975..1640)
                     /locus_tag="PKW44_03910"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011635256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HQR50763.1"
                     /translation="MHLALFDLDNTLLAGDSDFEWAEFLIEHGVLDRELYQARNAQFY
                     ADYKAGTLDIHAFLEFQLKPLSTHPRAQLEEWHALYMEKKVRPMMTTKSRALVEQHRN
                     NGDLLVIITATNSFVTGPIAREFGVEHLIGTTPEETGGQFTGRVSGIPSFKEGKITRL
                     NQWLEGRGQSLRSFETSCFYSDSLNDLPLLKLVDRPVAVNPDQTLRAHAEQMGWPIIS
                     LRE"
     gene            1705..2373
                     /locus_tag="PKW44_03915"
     CDS             1705..2373
                     /locus_tag="PKW44_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000041 - transition metal ion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HQR50764.1"
                     /translation="MNFPDNLLPQALLWAGDAAALLLLAGALLTAPWRRLARIDLLNV
                     WLGMCVGLMLIWGIKTGIKPGLNFHLLGATLMTLMFGSRLALLGFAIVLLGVTLSGAS
                     GWDSYAINLLSMGALPVLFSHWLYVLAERHLPNHLFVYIFLDAFIGAALAMSLSGVAA
                     TLLLVITGVYPYAYLAEHYLPYFMLMSWSEAMLTGMAITLMTAFRPQWLLTFDDFRYL
                     GRKS"
     gene            complement(2348..3244)
                     /gene="yedA"
                     /locus_tag="PKW44_03920"
     CDS             complement(2348..3244)
                     /gene="yedA"
                     /locus_tag="PKW44_03920"
                     /inference="COORDINATES: protein motif:HMM:NF008432.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HQR50765.1"
                     /translation="MHEQQKQRWLISVSLLALYLIWGATFLAMKIAIDSLPPFLMAAL
                     RFLGAGGLLYVLLRLRGVAAPTRPQWLGAAVVGTLLLACGNGGVAFAQQWVATGAAAL
                     MIATVPLWTVLFAGLWGMPPHRREWVGIGLGMLGVLILNLGNNLQASPLGAGILLLAA
                     VCWALGSIWGKRLPMPPGLMAPAAQMLAGGAVLVLASMASGERIVAPPTWKTLVALLY
                     LIAFGSLIAYSAYLYLLKTVRPALATSYAFVNPLIAMMLGTWLLNEHFGAHELGAMVL
                     ILLGVLLVLPINFSFRTSGRGT"
     gene            complement(3312..4847)
                     /locus_tag="PKW44_03925"
     CDS             complement(3312..4847)
                     /locus_tag="PKW44_03925"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HQR50766.1"
                     /translation="MQDKLIIFDTTLRDGEQSPGAAMTKEEKLRIARQLEKLGVDVIE
                     AGFAAASPGDFDAIRSIAEIIKTSTVCSLARASENDVRRAGEAIKPAKSGRIHTFIAT
                     SPIHMENKLRMTPDQVVERAVQAVKWAREYTDDVEFSAEDAVRSDIDFLVCVFDAVIQ
                     AGARTINVPDTVGYSIPALWGERFKQLLARVPNADKVVWSTHCHNDLGMAVANSLAAV
                     MNGARQVECTINGLGERAGNASLEEVVMAVKTRKDVFPCDSRIDTTQIVPTSKLVSSI
                     TGYPVQPNKAIVGANAFAHESGIHQDGVLKHRETYEIMRAEDVGWNANKLTLGKLSGR
                     NAFRTRLKELGIELESEDTLNAAFARFKELADKKSEIFDEDLHALVNDEFVSETPEHF
                     KLVYLHVCSETGETPHATVTVLENGAERRAEADGGGPVDASFKAIEQIATSGAELLLY
                     SVNNITTGTDAQGEVTVRLSKGGRIVNGQGADTDIVIASAKAYLNALNKLHSKLERAH
                     PQV"
     gene            complement(5086..5874)
                     /gene="pssA"
                     /locus_tag="PKW44_03930"
     CDS             complement(5086..5874)
                     /gene="pssA"
                     /locus_tag="PKW44_03930"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HQR50767.1"
                     /translation="MAEPTPRPRRKRPLLDPTLRERSIYLLPNLFTSAALFAGFYSVV
                     QAMGGRFEQAAVAIFIAMVMDGLDGRVARLTHTQSAFGAEYDSLSDMVSFGVAPALVL
                     YVWALQPMGKLGWIAAFIYCAGAALRLARFNTKLDESDKRYFQGLPSPAAAALLAGLV
                     WVMHDNGLKGTDIFFGWLHMKWIAWGFTVFAGLTMVSDLRYFSGKDFNLRRSVPFVVI
                     LLIVLGFVLVSYSPAEVLFASFVIYGLSGYAGWLMRRLNRPKVA"
     gene            5958..7061
                     /gene="tgt"
                     /locus_tag="PKW44_03935"
     CDS             5958..7061
                     /gene="tgt"
                     /locus_tag="PKW44_03935"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HQR50768.1"
                     /translation="MQFSQIQNDGAARRGTLTLAHGAVETPAFMPVGTYGTVKTLSPQ
                     EIRDIGAHIVLGNTFHLWLRPGLEVIAAHGGLHGFMGWDGPILTDSGGFQVWSLGALR
                     RITEEGVKFRSPINGDLCFLTPEESMRIQRVLNSDIVMIFDECTPYPATWTQARDSME
                     LSLRWAARSKAAHAGNPNALFGIIQGGMYEDLRDKSLQGLTGIGFDGYAIGGLSVGEP
                     KEDMLRILDHTVPQMPGDKPRYLMGVGTPEDLVDAVLRGVDMFDCVMPTRNARNGWLF
                     TRFGDVKLKNARYKADTGPLDPECSCYTCRHFTRAYLHHLHKVGEILGSRLNTIHNLH
                     FYQELMAGIRAAIEQGRLPAFAEEFKRRRQALC"
     gene            7099..7416
                     /gene="yajC"
                     /locus_tag="PKW44_03940"
     CDS             7099..7416
                     /gene="yajC"
                     /locus_tag="PKW44_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HQR50769.1"
                     /translation="MFISNAYAAGAAATPDWTSFLPLVIIFVLFWFMLIRPQMKQAKE
                     QRKMIDALQKGDEVTTAGGLAGRIIKVGDSFVGLEIAPETIVQVQKHTIQTLLPKGTL
                     KSL"
     gene            7438..9267
                     /gene="secD"
                     /locus_tag="PKW44_03945"
     CDS             7438..9267
                     /gene="secD"
                     /locus_tag="PKW44_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HQR50770.1"
                     /translation="MNRYPLWKYLLILAALLLGALYATPNFFGESPAVQVSPAKSSAK
                     ADTDLLGRVEAALKDSNLPYDAVFLDTSGVKVRFGDTDTQIKAKDILTNAMGKDYTVA
                     LNLVSRSPGWLYALGAKPMYLGLDLRGGVHFLMQVDMKAAVDKAAERYVGDIRTLLRE
                     KRIAYGGVVREGQSVQVKFKDVAERAKAEKEIGNNYPDLTLKESDSGEEKLLVASLGL
                     PAQKRIQDFALKQNIQTLHNRVNELGVAEPIIQQQGADRVVVQLPGVQDTAKAKEILG
                     RTATLEIRMVDEEKDDPATLQNAEKGQVPFGDEFFVERNGQPLLVKKQVVLTGEYITD
                     AGPGIDQQSGGSVVHVNFDGRGARIFKQVTRDNVGKRMAILLVEKGKAEVITAPVIRE
                     EIGGGRVQISGMANVQEATDVALLLRAGALAAPMEIIEERTIGPSMGEENIRRGVHST
                     LWGFSAIAVMMMIYYLVFGTFSVLALGVNLLLLVAILSLLQATLTLPGLAAIALALGM
                     AIDANVLINERVREEMRNGTTPQAAIHAGYDRAFGTILDSNVTTMIAGLALFLFGTGP
                     IKGFAVVHVLGILTSMFSAVLVSRAMVNLFYGYRRKIEKLTIG"
     gene            9281..10213
                     /gene="secF"
                     /locus_tag="PKW44_03950"
     CDS             9281..10213
                     /gene="secF"
                     /locus_tag="PKW44_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HQR50771.1"
                     /translation="MEIFRIKKDIPFMSYGRLTTSISLVTFILAVYFLATKGLALGVD
                     FTGGTVMEVSYPQTADIGKIRSAVDGIGLKDATVQNFGTSRDVLIRLPVKQGVTSAKL
                     SEEVLQALQNDNAQVEMRRVEFVGPQVGEELYENGALALLIVSLGIVLYLWMRFEWKF
                     GVAAIIANLHDVVIILGFFAFFQWEFNLTVLAAVLAVLGYSVNESVVVFDRVRENFRK
                     MRKASVTQVIDNAITTTMSRTVITHAMTQTMVCSMLFFGGEVLHNFALALTIGILFGI
                     YSSVLVASPIVMWLGVSREDLVKVEKKEIADATP"
BASE COUNT         1958 a         3079 c         3187 g         2179 t
ORIGIN      
        1 cggcttccag caaggccatg cgcacttcgc gcagggcctc ctggatgttg tcctcggtca
       61 agcgtgcctg accgcgcagg tttttgatga cgccagccag gcgcccggtt agattgtcga
      121 acatgctgtg tgctatattt gggaaaaatt gaattttacc ggaaccatgc tcgactttct
      181 gccttatatt gcggccgcgc tgttatatac cttggttgcc ttcctccagt ggcataccaa
      241 gaaattcgcc agcgggtggt atgagcgtgt ggcagtggcg acaggcttgg cgcttcatgg
      301 ctggctgctg gcgcatgata tgttcactgg caacggcgtc aatcttggcc tgattaacgc
      361 gctctccgct attttctggc tgacggtgct gatttactgg ctggccagcc tgaaatacaa
      421 cctgcacgcc ctgcaggcct tcgtcctgcc gcccgccaca ctgttcgtgc tgctgcagaa
      481 gttggtgccg gaaagccatg tgctgccgta taccggcgat gcgctgttcg gcgcgcatct
      541 ggtcatcgcc ctgctggcct acagtctgtt tacttttgcc gccctgcatg ccgggctgat
      601 ggcgattgcg gagcgtagct tgcataacaa gacgccgctg tttcgactgc ctgatttccc
      661 gcctatcatg cccatggagc ggctgctgtt ccagatcatc ggcatcggct tcgtgctgct
      721 gaccctgact ctggtcagcg gcattttctt taccgagcag ttgttccatc aggcgctaca
      781 gttcaaccac aagacggtgt tttccattgc ggcctggctg attttcggtg cgctgctggt
      841 cggccggcgc gtgcaaggct ggcgcggccg cgttgccgtg cgctggacgc tcggtggctt
      901 cgtgctgctg ctgctggctt acgtgggcag caagttcgtg ctcgaagtgc tgcttgggcg
      961 ataatccggg ttttctattc tcgcaggctg atgatcggcc agcccatttg ttcagcatgc
     1021 gcccgcaagg tctggtcagg gttcaccgcc accggccggt ccactagttt caataacggc
     1081 aggtcgttca gcgaatcgct gtagaagcag ctggtctcga aacttctcaa actttgcccg
     1141 cgcccttcca gccactgatt gaggcgcgtg atcttgcctt ccttgaagct ggggatgccg
     1201 gacacgcgcc cggtgaactg gccgccggtt tcttcagggg tagtgccgat cagatgctcg
     1261 acgccgaact cgcgtgcgat cgggccggtc acgaaactgt tggtggcagt gatgatgacc
     1321 agcaggtcgc cattgttgcg gtgctgctct accagcgcgc gtgatttggt ggtcatcatc
     1381 ggccgcactt ttttctccat gtacagcgcg tgccattctt ccagttgcgc ccttgggtgg
     1441 gtggaaagcg gcttcagctg gaattcgagg aaggcatgga tatccagcgt gccagccttg
     1501 tagtcggcat agaactgggc gttgcgggcc tggtagagtt cgcggtcgag cacgccatgc
     1561 tcgatcagga attccgccca ctcgaaatcg ctgtcgcccg ccagcaaagt gttatccaag
     1621 tcaaacaaag ccagatgcaa ggtcttctcc aatattccaa aaagtcgtaa agtttaaccg
     1681 agaatccagc cgcgatcatt caccatgaac tttcccgaca accttctccc tcaggctctg
     1741 ctgtgggcag gggatgcggc cgctttgctg ctgttggcgg gggctttgct taccgcgccc
     1801 tggcggcggc tggcgcgaat cgacctgctg aatgtctggc tggggatgtg cgtggggctg
     1861 atgctgatat ggggcatcaa gaccggcatc aagccggggc tgaacttcca cctgctgggc
     1921 gccaccctga tgaccttgat gttcggctcg cggctggcct tgctcggctt tgccatcgtg
     1981 ctgctggggg tgacgttgtc cggcgcgtcc ggctgggaca gctacgcgat taacctgttg
     2041 tcgatggggg cgttgccggt attgttcagc cattggctgt atgtcctggc cgagcgtcat
     2101 ttgcccaacc accttttcgt ctatatcttc ctcgacgctt tcatcggcgc ggcgctggca
     2161 atgtccttga gcggcgtggc ggcgacattg ctgctggtca tcaccggcgt ctacccatat
     2221 gcctacctgg ccgaacacta cttgccctat ttcatgctga tgagttggtc ggaggccatg
     2281 ctcactggga tggcgatcac gttgatgacc gctttccgcc cccagtggct gttgaccttc
     2341 gacgacttca ggtacctcgg ccggaagtcc tgaaagaaaa attgatcggc agcaccagca
     2401 gcacgccgag caggatcaac accatggcgc ctaactcgtg ggcgccgaaa tgctcgttca
     2461 acagccaggt gcccagcatc atggcaatca gcgggttgac gaaggcatag ctggtggcca
     2521 gcgccggcct gactgtcttc aacaggtaaa ggtaggcgct gtaggcgatc agcgagccga
     2581 acgcgatcag gtaaagcaag gccaccaggg ttttccaggt cggcggagcg acaatgcgct
     2641 cgccgctggc catgcttgcc agcaccagca cggcaccgcc tgccagcatt tgcgcagcag
     2701 gcgccatcag gccgggtggc atgggcaggc gcttgcccca gattgagccc agcgcccagc
     2761 acacggcggc caacagcagt atgccagcgc ccagtgggct ggcttgcagg ttgttgccga
     2821 ggttcaggat tagcacgccc agcataccga ggccgatacc gacccattca cgccggtgtg
     2881 gtggcatccc ccacagcccg gcaaacagca ccgtccatag cggtacggtg gcgatcatca
     2941 gcgcggcggc gccggtcgcc acccattgct gggcaaaggc cacgccgcca ttgccgcagg
     3001 ccagcagcag ggtgcctact acggcggctc caagccattg cggccgcgtc ggggcagcca
     3061 cgccgcgcag tcgcagcagg acgtacagca agccgcctgc gccgagaaag cgtaacgccg
     3121 ccattaggaa cggcggcagg ctgtcgatgg cgattttcat ggcgagaaat gtcgcgcccc
     3181 aaatcaggta gagggcgagc aggctgacgc tgatcagcca gcgctgtttt tgttgttcgt
     3241 gcatctttaa gttcctccaa acaaaacagg ccgcttgcgg cggcctgttt caccggcatg
     3301 ttcgcccttt gctacacttg cggatgcgcg cgctccagct tggagtgcag cttgttgagc
     3361 gcattcaaat aggctttggc cgaagcgatg acgatatcgg tatccgcgcc ctggccgttg
     3421 acgatgcgcc cgcccttgga caggcgtacg gtgacctcac cttgggcatc ggtgccggtg
     3481 gtgatgttgt tcaccgagta cagcagcaac tctgcgccgc tcgtagcaat ctgctcgatc
     3541 gccttgaacg aggcatccac cgggccaccg ccatcggcct cggcgcgccg ttcagcaccg
     3601 ttctccagca cggtgacggt ggcgtgcggc gtttcgccgg tttcggagca gacgtgcaaa
     3661 tacaccagct tgaagtgttc tggcgtttcg ctgacgaact cgtcgttgac cagggcgtgc
     3721 aagtcctcgt cgaagatttc cgattttttg tcagccaatt ccttgaagcg ggcgaaggcg
     3781 gcattgagcg tgtcttcgct ttccagttcg atgcccaatt ccttcaagcg ggtgcggaat
     3841 gcgttgcggc cggaaagctt gcccaaggtg agcttgttgg cgttccagcc tacatcttcc
     3901 gcccgcatga tttcgtaggt ctcgcggtgc ttgagcacgc catcctggtg gatgccggat
     3961 tcatgggcga aggcattggc gccgacgatg gccttattag gctgcaccgg gtagccggtg
     4021 atactggaga ccagtttcga ggtcggcacg atctgcgtgg tgtcgatgcg gctatcgcag
     4081 gggaatacat ccttgcgcgt cttcaccgcc atcacgactt cttccagact ggcgttgccg
     4141 gcccgctcgc cgagaccgtt gatggtgcat tccacctggc gcgcgccatt catcacggcc
     4201 gcgagcgagt tggccactgc cataccgagg tcgttgtggc agtgcgtgct ccatactacc
     4261 ttgtcggcat tgggcacgcg ggcgagaagc tgcttgaagc gttcgcccca caacgctggg
     4321 atggaatagc ccacggtgtc aggtacgttg atggtcctgg cgccagcctg gatcaccgcg
     4381 tcgaatacgc ataccagaaa gtcaatgtcg gagcgcaccg cgtcctcggc ggaaaactcc
     4441 acgtcgtcgg tgtactccct ggcccatttg acggcctgca ccgcgcgctc gaccacctgg
     4501 tcgggcgtca tgcgcagctt gttttccatg tggatggggc tggtggcgat aaaggtgtgg
     4561 atgcggccgg atttggcggg cttgatcgct tcgccggcgc ggcgcacgtc gttctcgctg
     4621 gcgcgcgcca aggagcacac ggtcgaggtc ttgatgatct cggcgatgct gcggatggcg
     4681 tcgaaatcac ccgggctggc ggcggcgaag ccggcctcga tcacgtccac gcccagcttt
     4741 tccaactgac gcgcgatacg gagcttttct tccttggtca tggcagcgcc ggggctttgc
     4801 tcgccgtcgc gcaaggtggt gtcgaatatg attaatttgt cctgcatggc attactccaa
     4861 attttcttta gccggagcgg ctataaattc atcactcgca tggtagcgga ttgtaccttg
     4921 cgaaccttgt aaaacttgaa ttgggggtgg tgttgtattt agcgcgcgac gcgcagcagc
     4981 agcccggcca ggaggaggcc gtagtgcggg agggacaagg tgacgtagcg gttgaaaaac
     5041 atagtgcggc gaatataaat gttttcccgg tttaacgcaa cagaatcaag ccacttttgg
     5101 ccggttcaac cgacgcatca gccagccggc gtatcccgac aggccgtaaa tcacgaacga
     5161 agcgaacagc acttccgccg ggctgtagga aaccagcacg aatcccagca cgatcaacag
     5221 aatgacgacg aacggcacgc tgcggcgcag gttgaaatcc ttgccgctga aatagcgtag
     5281 gtcactgacc atggtcaggc cggcaaagac cgtgaagccc caggcaatcc acttcatgtg
     5341 cagccagccg aaaaagatgt ctgtaccctt gagaccgttg tcatgcatga cccagaccag
     5401 gccggccagc agggctgcgg cagccgggct gggcaagccc tggaagtagc gcttgtcgga
     5461 ttcgtccagc ttggtgttga agcgtgccag tcgcagtgcg gcgcccgcgc agtagataaa
     5521 ggcggcgatc cagcccagct tgcccatggg ctgcagcgcc catacataaa gcaccagcgc
     5581 aggcgctaca ccgaacgaca ccatgtccga caggctgtcg tattccgcgc cgaacgcgct
     5641 ctgggtatgg gtcagccgcg ccacgcgccc atccagcccg tccatcacca tggcgatgaa
     5701 aatggcgaca gccgcctgtt caaaacgccc tcccattgcc tgcaccaccg aatagaagcc
     5761 ggcgaacagt gcggccgagg tgaacaggtt gggcagcagg tagatgctgc gctccctcag
     5821 ggtggggtcg agcaatggtc tcttgcggcg ggggcgtgga gtgggttcgg ccatggttga
     5881 ttcagctcag gttaactgcg gccagtatag caaaaccctg tggtagtatc gcgcatcctt
     5941 aagattgttc ctgttttatg caattctccc aaatccagaa tgatggcgca gcacggcgcg
     6001 gcacgctgac cctggcgcat ggcgcggtgg aaacgccggc gttcatgccg gtcggcacct
     6061 atggcacggt gaagaccctg tcgccacagg aaatccgcga catcggcgcg catatcgtgc
     6121 tgggcaatac cttccacctg tggctgcgac ccgggctgga ggtcatcgcg gcgcatggcg
     6181 gtctgcatgg gttcatgggc tgggacgggc cgatcctgac cgattccggc ggctttcagg
     6241 tgtggagcct gggggcgttg cgcagaatca cggaagaggg ggtcaagttc cgctcgccga
     6301 tcaatggcga tttgtgcttt ctgacaccgg aagagtcgat gcggatccag cgggtgctga
     6361 attccgatat cgtcatgatc ttcgacgagt gcacacctta cccggccacg tggacgcagg
     6421 cgcgcgactc gatggagctg tcgctgcgct gggcggcgcg cagcaaggcg gcgcacgccg
     6481 gcaaccccaa tgcgctgttc ggcatcatcc agggcggcat gtacgaggat ttgcgcgata
     6541 agtcattgca agggttgacc ggcatcggct tcgacggcta cgccatcggc gggctgtcgg
     6601 taggcgagcc caaggaagac atgttgcgca ttctcgacca caccgtgccg caaatgccgg
     6661 gggacaagcc gcgttattta atgggtgttg gcacaccaga ggacttggtg gacgcggtgc
     6721 tacgcggcgt ggacatgttt gactgcgtga tgccgacgcg caacgcgcgc aatggctggc
     6781 tgtttacccg gttcggtgat gtcaagctga agaatgcacg ctacaaggcc gacaccggcc
     6841 cgctcgaccc ggagtgttcc tgttacacct gccgccactt tacccgcgcc tatctgcatc
     6901 atttgcataa ggtgggcgaa atcctcggtt cgcgcctcaa caccatccac aacctgcatt
     6961 tttatcagga gctgatggcc ggaatacgtg ctgccatcga acaggggcgg ctgcccgcct
     7021 ttgccgagga attcaagcgt cgtcgtcagg ctttatgcta gaattgccaa attttttaac
     7081 gtcttagagg aaattcacgt gtttatcagc aacgcctacg ctgccggtgc ggcagctact
     7141 ccggactgga ccagcttcct gccgctggtc atcattttcg tcctgttctg gttcatgctg
     7201 atccgcccgc agatgaagca ggccaaggag cagcgcaaga tgattgatgc cctgcaaaag
     7261 ggcgatgaag tcaccactgc tggcggcttg gccggcagga tcatcaaggt gggcgatagt
     7321 ttcgtcgggc ttgaaatcgc gccggaaacc atcgttcagg tacaaaagca cactatccag
     7381 accctgctgc ccaagggcac cctcaagtcc ctctaaaatt cgcaaggacg cgctttcatg
     7441 aaccgctacc ccctgtggaa gtatctgctc atcctggcgg cactgctgct gggggcgctg
     7501 tacgccaccc ctaatttctt cggcgaatcc cccgccgtgc aggtgtcgcc ggccaagagt
     7561 tcggccaagg ccgataccga cttgcttggc cgagtggaag cggcgctcaa ggacagcaat
     7621 ctgccttacg atgccgtgtt tctcgatacc agcggcgtca aggtgcgatt cggcgatacc
     7681 gacacccaga tcaaggccaa ggatattctg accaacgcta tgggcaagga ttatactgtg
     7741 gcgctcaatc tggtctcgcg ttcgcccggt tggctgtatg cgcttggcgc gaagccgatg
     7801 tacctcggcc tcgacttgcg cggcggcgtg cacttcctga tgcaggtgga catgaaggcg
     7861 gcggtggaca aggccgctga acggtatgtc ggcgacatcc gcaccctgtt gcgcgagaag
     7921 cgcatcgctt atggcggcgt ggtacgcgag ggccagtcgg tgcaggtcaa gttcaaggac
     7981 gttgccgagc gtgccaaggc ggagaaggaa attggcaaca actatcccga cctcaccctg
     8041 aaggaatccg acagcggcga ggagaagctg ctggtcgcca gccttggcct gccggcgcaa
     8101 aaacgtattc aggatttcgc cctgaaacag aacattcaga cgctgcataa ccgcgtcaac
     8161 gaactggggg tggccgagcc catcatccag cagcagggtg ccgaccgcgt ggtggtgcag
     8221 ttgccgggtg tgcaggatac cgcaaaagcc aaggaaatcc tcggccgcac cgccaccctg
     8281 gaaatccgca tggtggacga ggaaaaggac gacccggcca cgctgcaaaa cgccgaaaag
     8341 ggacaagtgc cgttcggcga cgagttcttc gtcgagcgca acggccagcc gctgctggtg
     8401 aaaaagcagg tggtgctgac cggcgaatac atcaccgatg ccggtcccgg gatcgatcag
     8461 cagagcggcg gctcggtagt tcacgtcaat ttcgacggcc gcggcgcgcg cattttcaag
     8521 caagtgacgc gcgacaatgt cggcaagcgc atggccatcc tgctggtgga aaagggcaag
     8581 gccgaagtga ttaccgcccc ggtgattcgc gaggaaatcg gcggcggccg cgtgcagatt
     8641 tccggcatgg ccaacgtgca ggaagccacg gacgtcgcct tgctgctgcg tgccggtgcg
     8701 ctggctgcgc cgatggaaat catcgaggaa cgtaccatcg gtcccagcat gggggaggaa
     8761 aacatccggc gcggtgtgca ttccacgctg tggggcttct ccgccattgc cgtgatgatg
     8821 atgatctatt acctggtgtt cggcaccttc tcggtgctgg cgctgggcgt caacttgcta
     8881 ctgttggtgg ccattctgtc cctgctgcaa gccacgctta ccctgcctgg cttggcggct
     8941 atcgcgctgg ctctggggat ggcgatcgat gccaacgtgc tgatcaacga gcgggtgcgc
     9001 gaggagatgc gcaacggcac gacgccacag gctgccattc acgccggcta tgaccgcgcc
     9061 ttcggcacca ttctcgactc caacgtcacc accatgatcg ccggtctggc actgtttctg
     9121 ttcggcaccg gcccgatcaa gggcttcgcg gtagtgcacg tgctgggtat cctgacctcg
     9181 atgttcagcg cggtgctggt gtcgcgcgcc atggtcaacc tgttctacgg ctatcgccgc
     9241 aagatcgaaa aactgacgat cggctaagga agaaaaagcc atggaaattt tccgcatcaa
     9301 gaaagacatc cccttcatga gctatgggcg tctgaccacg tccatctcgc tcgtcacttt
     9361 tatcctggct gtttacttcc tcgccaccaa ggggctggcg ctgggtgtgg atttcaccgg
     9421 cggcacggtg atggaggtga gctacccgca aactgccgac atcggcaaaa tccgcagcgc
     9481 ggtggacggt atcggcctca aggacgccac ggtgcagaac ttcggcacca gccgcgatgt
     9541 gctgattcga ctgccggtca agcagggcgt gaccagcgcg aaactctcgg aagaggtgtt
     9601 gcaggcactg caaaacgaca atgcccaagt ggagatgcgc cgggtggaat tcgtcggccc
     9661 gcaggtgggc gaggagcttt acgaaaacgg tgcgctcgcg ctactgatcg tttccctcgg
     9721 catcgtgctc tacctgtgga tgcgcttcga gtggaagttc ggcgtggccg ccatcatcgc
     9781 caacctgcac gacgtagtca tcatcctcgg cttcttcgcc ttcttccagt gggaattcaa
     9841 cctcaccgtg ctggccgccg tgctggcggt gctgggttat tccgtgaacg agtcggtggt
     9901 ggtattcgac cgggtgcgcg agaatttcag aaaaatgaga aaagccagcg tgacgcaggt
     9961 catcgacaat gccattacca ctaccatgtc gcgtaccgtc atcacccacg ccatgacgca
    10021 gaccatggtc tgctccatgc tgttcttcgg tggtgaagtg ctgcacaact tcgccctggc
    10081 gctgactatc ggcattctgt tcggcatcta ttcctcggta ctggtggcca gccccatcgt
    10141 catgtggctg ggcgtttcgc gcgaagacct ggtcaaggtg gaaaagaagg aaatcgccga
    10201 cgccacgccc taaggcgtcc ttgacaagtt tccacgcttg cgcggaaact tgtccccatc
    10261 gcataacggt tttcaccgca cctcttggac gatattccct tatcctggca gctcggcgcg
    10321 cttgccgcgc tactgctgat tgccgctttc ttctcgatcg cggcaaccag cctgctgacg
    10381 ctcaaccgct accgcctgcg ccc
//
LOCUS       DAOMFA010000051        10364 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_681_length_10364_cov_4.878359, whole genome shotgun sequence.
ACCESSION   DAOMFA010000051 DAOMFA010000000
VERSION     DAOMFA010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 10364)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10364)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10364
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_681_length_10364_cov_4.878359"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     repeat_region   8..245
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=8..44
                     /rpt_unit_seq="gttgtagctccctctctcaccccggatgagctaccat"
     gene            complement(309..617)
                     /gene="cas2"
                     /locus_tag="PKW44_03955"
     CDS             complement(309..617)
                     /gene="cas2"
                     /locus_tag="PKW44_03955"
                     /inference="COORDINATES: protein motif:HMM:TIGR01573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HQR50772.1"
                     /translation="MRLLVLFDLPVVTKAERRAYTIFRRILINDGYDMTQFSVYGRLI
                     NGNDALEKHMKRLVDHLPQEGSIRCLAVTEKQYASMKLLVGLPLFQEKTVKSDQLLLF
                     "
     gene            complement(695..1600)
                     /gene="cas1"
                     /locus_tag="PKW44_03960"
     CDS             complement(695..1600)
                     /gene="cas1"
                     /locus_tag="PKW44_03960"
                     /inference="COORDINATES: protein motif:HMM:TIGR03639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR-associated endonuclease Cas1"
                     /protein_id="HQR50773.1"
                     /translation="MTWRSLVISHPAKLRREHYSLAIEQEQTAFVPFEDIAVILLNHR
                     EITLTHPVLSACGEYGISLFATGDTHHPSGVFLPYLPHSRATRWMRLQLDLPRPTAKQ
                     TWAAIARRKIANQAKCLRLAGREGDDRLESYVNRVRSGDSDNMEGQAAALYFPQLFGK
                     GFRRVEDSFVNAALDYGYAVLRGTIARGLVAHGMLPAFGIFHASEQNAFNLADDLIEP
                     FRPLVDLCVKNMRPSPDDELQPQDKVALVNLLNVDVAMPRGKMSVLSAIEQTVESLAR
                     IYEGGDAGLLELPALIGLEQHRLEY"
     gene            complement(1587..5003)
                     /gene="cas9"
                     /locus_tag="PKW44_03965"
     CDS             complement(1587..5003)
                     /gene="cas9"
                     /locus_tag="PKW44_03965"
                     /inference="COORDINATES: protein motif:HMM:TIGR01865.1"
                     /note="Cas9, originally named Csn1, is the large,
                     multifunctional signature protein of type II CRISPR/Cas
                     systems. It is well known even to general audiences
                     because its RNA-guided endonuclease activity has made it a
                     popular tool for custom editing of eukaryotic genomes;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR RNA-guided endonuclease Cas9"
                     /protein_id="HQR50774.1"
                     /translation="MTSKNKNLGPLTFGLDIGIASVGWAVLGENRIINLGVRCFDKAE
                     TADKGESLNLARRMARLLRRRLRRRAWRLTKLARLLKHGGVIGDVNLLKVSPGKGTPA
                     AQPWQLRVEGLDRKLESEEWARVLYHLCKHRGFHWMSKAEEKKADDDKEGGAVKKGLA
                     GTKKLMLEKSYRSAAEMVLAEFPDAQRNKRGDYSKALSRVLLAEEMQHLFEQQRKFGN
                     PHATAQLEAALLDRKTGLFWAQKPALSGEALLKMLGKCTFEKGEYRAPKASFTAERHV
                     WLTRLNNLRIVVDGKTRSLTEAERRFALPLPYLQAGDFSYKQLGNALVKEGFLQKEHF
                     KFAGLSYPSDKQKEEEKTKNPEDEKLVKLPGWQELRKALKEAGLDDEWQKISVAALDG
                     QPQLLDEIARVLSVYKEDEEVERELGKLDLPNKTAMMEALLTTRFDKFSNLSLKALYN
                     ILPHMEAGLRYDEACTLAGYHHSQLHEVGAGEHKTLPPFYAGRDKDGRLKFVDDMDIP
                     RNPVVLRALNQARKVVNALIKVYGSPHEVHIEMARDLSRNFDERRDIKKSQDEFRDRN
                     EKDRAAFNTEYNTSGELKGREFEKWQLYREQHGKCAYSLEPLDINRLFEMGYAEIDHA
                     LPYSRSFDDSKNNRVLVLAAENRNKGNMTPFEYLDGKTNSERWRQFVAFVESNKTYRQ
                     AKRNRLLKKDFGEEEAQSFRDRNLNDTRYICRFFKNYVERYLKLADDSEAKRCVVVSG
                     QLTSFLRARWGLVKVRGDSDRHHALDAAVVAACSHGMVKRLSDYSRRKELEKAREGFI
                     DMETGEIVNPAMLDRLEQHFPAPWPPFRQELLARLNEDNPAALRAEIEHLGSYPPEAL
                     DELHPLFVSRAPQRRNSGAAHKETIYAQPERLKTQGGVTQKVAVTSLKTSDIDKLIDP
                     HRNEKLYASLRSWVEKREDREKRAREVEKSAKEAKRELTADEKADIERLRALLRKPDK
                     NGNPTGPIVRTVTMVVDKLSGIPIRGGVAKNDTMLRVDVFSKAGKFHLVPVYVHHTVT
                     GLQNRAIVAFKDEDEWTLVDDSFQFCFSLYPNDFVRVTLKKEQFSGYYSGTDRATGAI
                     GLWAHDRNASVGKGGLMRVGVKMANSVEKFNVDVLGNVYPAPAEIRRDLA"
     gene            5197..5823
                     /locus_tag="PKW44_03970"
     CDS             5197..5823
                     /locus_tag="PKW44_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HQR50775.1"
                     /translation="MTDFNPSCRRCPRLATFLDEVKTREPGYFARPVPPFGDAQPKLL
                     IVGLAPGMHGANRTGRPFTGDYAGILLYQTLHKYGYASAPVSVSADDGLVLSGCRITN
                     AVKCLPPENKPEGREVTQCNGYLAAEIAALPRDAAILALGNVAHAAVLKALGLKASAY
                     RFGHAARHALPDGRILYDSYHCSRYNTQTRRLTEAMFHAVFESIVKGV"
     gene            5825..6007
                     /locus_tag="PKW44_03975"
     CDS             5825..6007
                     /locus_tag="PKW44_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase family protein"
                     /protein_id="HQR50776.1"
                     /translation="MPYVNVKLAGSVTREQKAKIAEEITATLQRHAHKPPQYTYITFE
                     EVDYEDWAIAGKLLDE"
     gene            6150..7967
                     /gene="uvrC"
                     /locus_tag="PKW44_03980"
     CDS             6150..7967
                     /gene="uvrC"
                     /locus_tag="PKW44_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HQR50777.1"
                     /translation="MSEEKPGFDPKPILKTLPNLPGVYRMINVQDEVIYVGKARDLKK
                     RVSSYFQKTLPSPRTRMMVSQIARIETTVTRSEAEALLLENNLIKSLMPRYNVLFRDD
                     KSYPYIMLSGGEFPRLAFHRGTQQKGSRYFGPFPNAVAVRESIQLLQKVFRLRTCENS
                     VFSNRSRPCLQYQIERCTAPCVGYIAAEDYRHDVEHAAMFLEGREQQVIEDLSTCMTA
                     AAEQQKYELAAVYRDRIQSLRQVQARQFVSDFRSGDADVVACVEERGAMCVNLVMIRG
                     GRHVGDRSYFPKNIEDCDTQTVLQAFLEQHYLQQPVPPLIITGVALEQDGLEEILGER
                     AGKKVQISAYAGGDRRVWLKMAETNAELALRQHLAQKTTQEGRLLALREALDLPESAE
                     RIECFDISHTMGEATVASCVVFDKGAMQSSEYRRYNIEGITAGDDYAAMKQALTRRYT
                     KIASGEGKLPDLIFIDGGKGQLGVAVEVMQEVGLPDVLLVGIAKGEERKSGLEQLIFP
                     GRDDALRLPKDNPGLHLLQQIRDEAHRFAITGHRGRRGKARTTSSLEEIGGVGAKRRQ
                     KLLTRFGGLAGVLKASVDELAQVEGISQTLAEKIYQELH"
     gene            7973..8542
                     /gene="pgsA"
                     /locus_tag="PKW44_03985"
     CDS             7973..8542
                     /gene="pgsA"
                     /locus_tag="PKW44_03985"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018507554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HQR50778.1"
                     /translation="MHWNIPTLLTWLRIVLIPVFVGIFYLPPDVIPAHWVNFSATLIF
                     AVAAVTDWLDGWLARRLNQTSAFGAFLDPVADKLMVAAALIVLVELGRVGAVVALIII
                     GREIAISALREWMASLGEGGSVAVAMVGKLKTSAQMAAILFLLYAEPILGINSLQVGT
                     LLIYLAVVLTLASMLHYLKAARDQLRQAA"
     gene            8617..>10364
                     /locus_tag="PKW44_03990"
     CDS             8617..>10364
                     /locus_tag="PKW44_03990"
                     /inference="COORDINATES: protein motif:HMM:NF015803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HQR50779.1"
                     /translation="MNKHKKNSVIPRSMRALPSLHGLVFSLLLIFCGESVLAATPPRP
                     NAGSLLNQTTPPTDEPTLPSSSIPALLDEKAEEADVEGVKIKVSKFVIDDARLLPEAL
                     LQAQLADLVGQELSLAGLRQAAARITKTYREHGYFLARAYLPAQEITDGVVHIAVFEG
                     KYDGVEAGDSPRLNRQHAQGILDAHQVAEGQPIERSELERSLILMEQRSGAPVQALLQ
                     PGATVGTSHMVIEAPSGPLFSGQLGVDNYGNRYSGEERATAQFSLNSPRGVGDRLSTW
                     LMKSSDSDAVFAAYQTPVGYRGLTLGASYSKFNYGELCCEFKALEEKGSAEVFGAQAR
                     YPLILSQRAIMNAGLSLERKRLRDTSAVGELDNKRADVVSFSLDGVNSAMRGQNRYLA
                     TLSAGDLNLNQNPASASFDAATLNTSGDYLKLRAEFEHLHPFANGTKLDLRLSGQASD
                     RNLDSSEEFILGGSGGIRAYPEGEATGDEALLMRLDWLIPVNFQQLPGSLMARLFFDA
                     GTVWINRDTGNGLGYPGVPNNYGLTGAGFGFTWNLPKGFTANLDAATMIGSTPGRAVN
                     GDNADGRDNRSRAWMGL"
BASE COUNT         1932 a         3029 c         3152 g         2251 t
ORIGIN      
        1 agcctcagtt gtagctccct ctctcacccc ggatgagcta ccattggatg gcaggaataa
       61 gcgaaggcat agaagttgta gctccctctc tcaccccgga tgagctacca tcctcactag
      121 accgttattc cctcctcgat ggttgtagct ccctctctca ccccggatga gctaccattg
      181 catcttgaac gttccgccct tgtcaggcgt tgtagctccc tctctcaccc cggatgagct
      241 accataggcc tcaagcaaac gcccgccaga tgtgaatctg gcgggcgttc tgctgtaaaa
      301 atctggtttc aaaatagtaa gagctggtcg gatttgacag ttttttcctg aaaaagcggc
      361 aggccgacca gcagtttcat gctggcgtat tgcttttcgg tgacggccag gcagcgaatg
      421 gagccttcct gcggcaggtg gtctaccagc cgtttcatat gcttttccag cgcgtcgttg
      481 ccgttaatca gacgtccgta gaccgagaac tgggtcatgt cgtagccgtc gttgatgaga
      541 atccggcgaa atatggtata ggctcggcgt tcggccttgg tgaccacggg gaggtcgaag
      601 agtaccagca gtctcatgaa tcgcttgttc tccgtgctca tggaatgttg ttatcccctc
      661 accccaaccc ctctcccgct tgcgggcgag gggcttaata ctccagccga tgctgctcca
      721 gcccaatcag cgcaggaagt tccagcaacc ccgcatcgcc gccttcgtat atccgtgcca
      781 gactttccac ggtctgttcg atggcagaaa gtactgacat ttttccgcgc ggcatggcga
      841 catccacgtt gagcaggttg accagtgcta ccttgtcctg cggttgcaat tcgtcgtcgg
      901 gggatgggcg catgtttttc acgcacaaat ccaccagcgg gcggaaaggt tcgatcaggt
      961 cgtcggccag gttgaaggcg ttctgctcgc tggcgtgaaa gatgccgaaa gcgggcaaca
     1021 tgccgtgcgc caccagcccg cgcgcaatcg tgccgcgcag cacggcatag ccgtaatcga
     1081 gggcggcatt gacgaaactg tcttccacgc gccggaaccc cttgccaaat agctggggga
     1141 agtagagcgc ggcagcttgc ccctccatgt tgtcgctgtc gccggagcgc acgcgattca
     1201 cgtaggattc cagccggtca tcgccctcgc gcccggcgag gcgcaggcat ttggcttggt
     1261 tggcgatctt gcgccgcgcg atggccgccc aggtttgctt ggcggtaggg cgcggcaaat
     1321 ccagttgcag tcgcatccag cgggtggcgc gtgaatgcgg caggtagggc aggaacacac
     1381 cgctggggtg atgtgtatca ccggtggcaa acaggctgat gccatattcg ccgcaggcgg
     1441 agagcaccgg atgggtgagc gtgatttcgc ggtggttgag caggatgacc gcaatgtcct
     1501 cgaacggcac aaaggccgtt tgctcctgct cgattgcaag ggagtaatgc tcgcgccgca
     1561 gcttggccgg gtgagagatg acgaggctac gccaggtcac ggcgtatctc cgctggggcg
     1621 gggtagacgt tgccgaggac atctacgttg aatttttcaa cagagtttgc cattttaacg
     1681 cccactctca ttaacccacc tttgccaacg cttgcgttcc tgtcgtgtgc ccaaagacca
     1741 attgcgccag tggctcggtc ggttcctgag tagtacccgg agaattgttc cttctttaat
     1801 gtgacgcgta caaaatcatt cggatacaac gaaaaacaaa actggaaact gtcatcaacc
     1861 agcgtccatt cgtcttcgtc cttgaacgcg acaatcgccc gattttgcag tcccgttacc
     1921 gtgtgatgta catataccgg caccagatgg aacttgccgg ctttgctgaa cacatccacg
     1981 cgcagcatgg tgtcgttctt ggcgacaccg ccacgaatcg gaatgccgga gagcttgtcc
     2041 accaccatcg tcacggtacg cacgatgggg ccggtgggat tgccgttctt gtcaggcttg
     2101 cgtagcaggg cgcgcaggcg ttcgatgtcg gctttttcat cagcggtgag ttcgcgcttg
     2161 gcttccttgg cggatttttc aacttctctt gcgcgctttt ctctatcctc acgtttttcc
     2221 acccatgacc gcagggaggc atagagtttt tcgttgcggt gtggatcgat cagcttgtct
     2281 atgtcgctcg ttttcagact ggtgacagcc accttttgag tgacgccccc ctgcgttttc
     2341 aggcgctctg gctgggcata gatggtttcc ttgtgtgccg caccgctgtt gcgccgttgc
     2401 ggagcgcgcg agacaaacag gggatgcagt tcgtccagtg cttctggcgg atagctgccg
     2461 aggtgttcaa tttcagcacg cagggctgcc ggattgtctt cgttgagccg tgccaatagc
     2521 tcttgccgga aaggtggcca cggagcgggg aagtgttgtt ccagccggtc aagcatggca
     2581 gggttcacga tttcgccagt ttccatgtcg atgaagcctt cacgtgcttt ttccagttcc
     2641 ttgcggcgcg agtaatcgga aagccgcttg accatgccat ggctgcaagc cgcgaccacg
     2701 gcggcatcca gcgcatggtg gcggtcgctg tcgccacgca ctttgaccag cccccagcgg
     2761 gcgcgcagga aggatgtcaa ctggccgctg accaccacgc aacgcttggc ttcgctgtca
     2821 tctgccaact tgagataacg ctcgacgtag ttcttgaaga aacggcagat atagcgggtg
     2881 tcgttgaggt tgcgatcacg gaatgattgg gcttcctcct cgccaaaatc ctttttgagc
     2941 aggcggttgc gcttggcctg gcggtaagtt ttgttggatt caacaaaagc cacgaactgc
     3001 cgccagcgct cgctgttggt tttgccgtcc agatattcaa acggcgtcat gttgcccttg
     3061 ttgcggttct cggcggcgag taccagtacg cggttgttct tgctgtcgtc aaagctgcgc
     3121 gaatagggca gggcgtggtc tatttcagca tagcccattt caaacagacg gttgatatcc
     3181 agcggctcca gtgagtaagc gcatttgcca tgttgttcac gatagagctg ccatttctcg
     3241 aattcccggc ctttcagttc gccactggtg ttgtattcgg tgttgaaggc agcacggtct
     3301 ttttcgttgc ggtcgcggaa ttcgtcctgc gattttttga tgtcgcgacg ctcatcgaaa
     3361 ttacgagaca gatcccgtgc catttcgata tgcacctcat gcggtgagcc gtaaaccttg
     3421 ataagcgcat tgaccacctt gcgcgcctga ttgagcgcgc gcagcacaac cgggttgcgc
     3481 gggatgtcca tgtcatcaac gaatttcagg cggccatcct tgtcgcgccc ggcatagaag
     3541 ggcggcaggg ttttatgctc gcccgcaccg acctcatgca actggctgtg gtggtagccc
     3601 gctagtgtgc aggcttcgtc gtagcgtagt cccgcttcca tatgcggcag gatgttgtaa
     3661 agggctttta gcgacaggtt gctgaatttg tcgaagcggg tggtgagtaa ggcttccatc
     3721 atcgcggtct tgttcggcaa atccagcttc ccgagttcgc gctcaacttc ttcgtcctcc
     3781 ttgtaaacag aaagcacgcg ggcgatttcg tccagcagtt gcggttggcc gtccaacgcg
     3841 gcgacggaga ttttttgcca ttcgtcatcc agcccggctt ctttcagagc tttgcgcaat
     3901 tcctgccaac cggggagctt gaccagtttt tcgtcttccg ggtttttggt tttttcttct
     3961 tctttttgtt tgtcgcttgg gtacgagagg ccagcgaact tgaagtgttc cttttgcagg
     4021 aaaccctcct ttaccagcgc gttgccaagc tgcttgtaac tgaagtcgcc agcttgcaga
     4081 taaggcaatg gcagcgcaaa gcgccgctct gcttcagtta atgagcgtgt cttaccatct
     4141 accacgatgc gcagattgtt gaggcgcgtc agccagacat ggcgctcggc ggtgaagctg
     4201 gctttgggcg cgcggtattc gcctttctca aaggtgcatt tgcccaacat tttgagcagc
     4261 gcctcgccgg acagcgcggg cttttgcgcc cagaacaacc cggtttttct gtcaagcagt
     4321 gccgcttcaa gttgggccgt agcgtgcggg ttgccgaatt tgcgctgttg ttcgaacagg
     4381 tgctgcattt cttccgccag taacacgcgc gaaagcgcct tgctataatc gccacgcttg
     4441 ttgcgctggg cgtcggggaa ttcggccaac accatttcgg cggcgctgcg gtagcttttt
     4501 tccagcatga gctttttcgt gccagccagc cctttcttga ccgcgccgcc ttccttatcg
     4561 tcgtcggctt tcttttcctc ggccttgctc atccagtgaa agccgcgatg cttgcagagg
     4621 tgatacagca cgcgcgccca ttcttcggat tcaagcttgc ggtcgaggcc ttctacacgg
     4681 agttgccaag gctgtgcagc gggtgtgcct ttgccgggag ataccttgag caaattgaca
     4741 tcgccaatca caccgccgtg cttgagtagt cgtgccagtt tggtgagccg ccaagcgcgg
     4801 cggcgcaagc ggcggcgaag cagccttgcc atgcggcggg caaggttgag tgattcaccc
     4861 ttgtcggcgg tttccgcctt gtcgaagcag cgcacgccaa ggtttatgat gcggttttcg
     4921 cccagtaccg cccagccgac cgaggcgatg ccgatgtcca gaccgaatgt gagggggccg
     4981 agatttttat tcttgcttgt catgcctgtt ctccgtaata ttccaatcgt tgtagttcat
     5041 ccggggtgag agccgttgct acaataaaga atcgaaagat tctgtatcca agcccggcgg
     5101 ggcaacccgc cgggttttcc atttgcaagc cagcatataa ccggaagcca agatctttca
     5161 agaggttatt ttctggtttc taggttttgg aactaaatga ctgactttaa cccctcctgc
     5221 cgccgctgcc cgcgtcttgc cacttttctg gacgaagtaa aaaccaggga gccgggctat
     5281 ttcgcccgtc cggtgccgcc gtttggcgat gcgcagccga agttgctgat cgtcgggctg
     5341 gcgcccggca tgcatggggc caaccgcacc ggccggccgt ttaccggtga ctatgccggc
     5401 atcctgctgt accagacgct gcacaagtat ggctatgcca gcgcgccggt ttcggtgtcg
     5461 gcggacgatg ggctggtgtt gagcggttgc cgcatcacca atgcggtgaa atgcctgccg
     5521 ccggagaaca agccggaagg gcgcgaggtg acgcagtgca acggctacct ggcggcggag
     5581 attgccgcgt tgccgcgtga tgccgccatt ctggcgctgg gcaatgtggc gcatgcggcg
     5641 gtgctgaagg cgcttggcct caaggcctcc gcctaccgct ttggacatgc cgcgcgccat
     5701 gcgttgccgg atggccgcat cctctacgac agttaccatt gcagccgcta caatacgcag
     5761 accaggcgcc ttaccgaggc gatgttccat gcggtttttg aatcaattgt gaaaggagtc
     5821 tgaaatgcct tacgtcaatg tcaagctggc gggcagcgtg acccgcgaac agaaggcgaa
     5881 gatcgccgag gaaatcaccg ccaccctgca gcgccatgcg cacaagccgc cgcagtacac
     5941 ctacatcacc ttcgaggagg tggattacga ggattgggct atagcaggga aactcctcga
     6001 cgaataggaa tgcaaaacac tttgcttgcc gatgcggtgt tgcgaacgcg gccagaaatc
     6061 ctcatttact gctatgtaaa ctccggtttc atgccgtatt cgcgccttgc ctcggcggcg
     6121 ctcgggtgtt ttgcaagggt ttgaaagtgg tgtctgaaga aaaaccgggg ttcgacccca
     6181 agcctatcct gaaaacattg cccaacctgc cgggcgttta tcgcatgatc aacgtgcagg
     6241 atgaggtgat ttacgtcggc aaggcgcgcg atttgaaaaa gcgcgtttcc tcctacttcc
     6301 agaaaaccct gcccagcccg cgcacgcgca tgatggtgtc gcagatcgcg cgcatcgaga
     6361 ccacggtgac gcgctccgag gcggaggcgc tgctgttgga gaacaacctc atcaagtcgc
     6421 tgatgccgcg ctacaacgtg ctgttccgcg acgacaagtc ctatccctac atcatgctgt
     6481 ccggcggcga gttcccgcgc ctggccttcc atcgcggcac gcagcagaag ggcagccgct
     6541 acttcggccc gttccccaat gcggtggcgg tgcgcgagag catccagctg ctgcagaagg
     6601 tgttccgcct gcgcacctgc gaaaactcgg tgttcagcaa ccgctcgcgg ccgtgtttgc
     6661 aataccagat cgagcgttgc actgcgccgt gcgtgggtta tattgcggca gaagattacc
     6721 gacatgacgt ggagcatgcg gcgatgttcc tggaagggcg cgagcagcag gtgatagagg
     6781 atctttccac gtgcatgacg gcggcggcgg agcagcagaa gtacgagctg gcggcggtgt
     6841 accgcgaccg catccagagt ctgcgccagg tgcaggcgcg ccagttcgtc agcgattttc
     6901 gcagcggcga cgccgacgtg gtggcctgcg tggaggagcg cggcgccatg tgcgtcaacc
     6961 tggtgatgat ccgcggcggg cggcacgtcg gcgatcgcag ttatttcccg aaaaatatcg
     7021 aggattgcga cacgcaaacc gtgttgcagg ccttcctcga gcagcattac ctgcaacagc
     7081 cggtgccgcc gctcatcatc accggcgtgg cgctggagca ggacgggctg gaagaaatcc
     7141 tgggcgagcg cgccggcaaa aaggtgcaga tcagcgccta tgccggcggc gaccgccgcg
     7201 tctggctgaa gatggcagag accaatgccg aactggcgct gcgccagcac ctggcgcaga
     7261 agaccacgca ggaaggccgg ctgctggcgc tgcgtgaggc gctggatttg ccggaaagcg
     7321 cggagcgcat cgaatgcttc gacatcagcc acaccatggg cgaggcgacg gtggcgtcgt
     7381 gcgtggtatt cgacaagggc gcgatgcaga gttcggaata ccgccgctac aacatcgagg
     7441 gcattaccgc tggcgacgac tacgcggcga tgaaacaggc attgacgcgg cgctatacca
     7501 agatcgcctc gggcgagggc aagctgccgg acctgatctt catcgacggt ggcaaggggc
     7561 agttgggggt ggcggtggag gtgatgcagg aggtcggctt gccggacgtg ctgctggtgg
     7621 gtatcgccaa gggcgaggag cgcaagtcgg ggctggagca actgattttc cccggccgcg
     7681 acgacgcgct gcggctgccc aaggacaacc ccggcctgca cctgctgcag cagatccgcg
     7741 acgaggcgca ccgcttcgcc attaccgggc atcgcggacg gcgcggcaag gcgcgcacca
     7801 cgtcgtcgct ggaggaaatc ggcggcgtcg gcgccaagcg gcggcagaaa ctgctgaccc
     7861 gcttcggtgg gctggccggc gtactcaagg ccagtgtgga cgagcttgcg caagtcgagg
     7921 gcatcagcca aactctggct gagaagattt atcaggaact gcattgatgc atattcactg
     7981 gaatatcccc accctgctga cctggctgcg cattgtgctg atcccggtat tcgtcggcat
     8041 tttttacctg ccgcccgatg tgatccccgc gcattgggtt aatttttccg ccacgctgat
     8101 ttttgccgtt gccgccgtca ccgactggct cgatggctgg ctggcgcgcc gcctcaacca
     8161 gacttcggcc ttcggcgcct ttctcgaccc ggtggcggac aagctgatgg tggcggcggc
     8221 attgatcgtg ctggtcgaac ttggccgcgt cggcgccgtg gtcgcgctca tcatcatcgg
     8281 ccgcgagatc gccatttcgg cgctgcgcga gtggatggcg tcgctgggag aaggcggcag
     8341 cgtggcggtg gccatggtcg gcaagctgaa aacatcggcc cagatggctg ccatcctttt
     8401 cctgctgtac gccgagccga ttctggggat caattccttg caggtgggta cgctgctgat
     8461 ctatctggcc gtcgtcctga cgctggcttc catgctgcac tacctcaagg ctgcccgcga
     8521 tcagttgcga caggcggctt gattgctatt ttaggccttg tgggggtggc gctcctgcga
     8581 aagtggcgta aaatgccgga cttggttgac cccgccgtga ataaacacaa aaaaaacagt
     8641 gtcatacccc gctcgatgcg cgccctgccg tcgctgcacg ggctcgtctt ttcgcttttg
     8701 ctgattttct gcggcgagag cgtattggcc gccacgccgc cacggcccaa tgccggcagc
     8761 ctgctgaacc agaccacgcc gcctaccgat gagccgaccc tgccatcctc cagtatcccg
     8821 gcgctgctcg atgaaaaggc ggaagaagcg gatgtcgagg gcgtcaaaat caaggtttca
     8881 aagttcgtca tcgacgatgc caggctgctg ccggaggcgc tgctacaggc gcaattggct
     8941 gacctggtcg gccaggagct cagccttgcc ggcctgcgcc aggctgccgc gcgcattacc
     9001 aagacttatc gtgagcacgg ctattttctc gcccgcgcct acctgccggc gcaggagatc
     9061 accgacggcg tggtgcatat cgcggtgttc gagggcaagt acgacggcgt ggaggccggc
     9121 gactcgccgc ggctgaaccg gcagcatgcg cagggcattc tggatgcgca tcaggtagcc
     9181 gagggacagc ctatcgagcg ttcggaactg gaacgcagcc tgatcctgat ggagcagcgt
     9241 tccggcgcgc cggtgcaagc cctgttgcaa ccgggcgcga ctgtcggcac ctcgcacatg
     9301 gtgatcgagg cgccatccgg gcccttgttc agcggccagt tgggcgtgga taactatggc
     9361 aatcgttaca gcggcgagga gcgcgccacc gcgcaattca gcctcaacag cccgcgcggc
     9421 gtcggcgacc gcctcagcac ctggctgatg aagtcctccg attccgacgc ggtatttgcc
     9481 gcctaccaga ccccggtggg ctatcgtggc ttgacgctgg gcgccagtta ttccaagttc
     9541 aactacggcg agctctgctg cgaattcaag gccctggaag aaaaaggcag cgccgaagtc
     9601 tttggcgccc aggcgcgtta cccgctgatc ctcagccagc gcgccatcat gaatgccggc
     9661 ttgagcttgg agcgcaagcg tttgcgcgac acctccgccg tcggcgagtt ggacaacaag
     9721 cgcgccgacg tggtgagctt ttcgctggat ggcgtaaata gcgccatgcg cggtcaaaac
     9781 cgctacctcg ccaccttgtc ggccggcgac ctcaacctga accagaatcc ggctagtgcc
     9841 agttttgatg ctgccacgtt gaacacctcc ggtgattacc tcaagctgcg tgccgaattc
     9901 gaacatctgc acccgttcgc aaacggcacc aagctcgatc tccggttatc cggccaggcc
     9961 agcgaccgca atctggactc ttcggaagag ttcatcctgg ggggaagcgg cggcatccgc
    10021 gcctacccgg aaggtgaagc caccggtgac gaggcgctgc tgatgcggct ggattggctt
    10081 attccagtaa acttccagca attgcccggc agcctgatgg cgcggctgtt cttcgatgcc
    10141 ggaacggtct ggatcaaccg ggatacgggc aacggcctgg gctatcccgg cgtgcccaac
    10201 aactacgggc tgactggcgc cggtttcggc tttacctgga atttgccaaa aggcttcacc
    10261 gccaatctgg atgccgccac catgatcggc agcacccccg gacgcgctgt caacggcgac
    10321 aacgccgacg ggcgcgacaa ccggtctcgc gcatggatgg gcct
//
LOCUS       DAOMFA010000052        10140 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_707_length_10140_cov_4.618146, whole genome shotgun sequence.
ACCESSION   DAOMFA010000052 DAOMFA010000000
VERSION     DAOMFA010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 10140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10140
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_707_length_10140_cov_4.618146"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2235
                     /locus_tag="PKW44_03995"
     CDS             <1..2235
                     /locus_tag="PKW44_03995"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQR50780.1"
                     /translation="RSLLAGLSLFCLAPSAVAGTTPASGEYTSQEAEQANVLLTSPVV
                     VTATRQEQNSFDLPVSIDVVDAATIHDAKLQVNISEDLPRVPGVVAQFRGQYAQDLQI
                     STRGFGARSQFGVRGIRLYSDGIPLTMPDGQGQTGTMDLLTAERIEVMRGPFSALYGN
                     SSGGVLQIFTADGPKNPTVDAAFMAGSYDTQREAIKVGGTEGALNYNAGLSHLESDDY
                     RDHASVRRTLFNTKLGLQVNEDTRLTVLGTYLDQPEAQDPLSLNKDQWEQDPKQAGIG
                     AENFNTRVIRRHTQVGAKLEHDFDADNTIHLMAYGGNRFNEQFQAVGANPSGTGITSA
                     FLKNLPPNANNQTAGGGAQIQRNFGGTDLHYTHKGLLAGNPYSFTVGANYDTQTDDRT
                     GFDNFLAIPGTGNNRICGVGVVCGVKGILRRDEDNKVWDFDQYAQGEWSPHERWNLFA
                     GVRHSDVHFENDDKYLYNGNSSGKVSHEKTTPVAGVLFKVTPMLNLYANAGKGFETPT
                     LVEMAYKPGGIGTFNLDLQPSTSDNYEVGAKAYLGADTRANVAFFHIDTNDEIVVSDT
                     TNGRSSYQNAASTRRQGVEVSIDSDFGYGFTGYASYAYLNAEYSSSFCNASTASNCAG
                     GTLVQSGKTIPGTYSDTAYAELAWKYAPSGFSTALEARAYSQVYVADTNNDTAPGYAL
                     MSWRGGFNQNIRQWRMSEFVRVDNLFDREYVGSVKLNDSNQRYYESGSNRNWLLGLNA
                     SYQF"
     gene            2379..3182
                     /locus_tag="PKW44_04000"
     CDS             2379..3182
                     /locus_tag="PKW44_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020183009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein dehydrogenase-associated SoxYZ-like
                     carrier"
                     /protein_id="HQR50781.1"
                     /translation="MSISKSLSLLTVISGLCLAYSGSALAEADEKLWPALKESFFAGK
                     TIENADFIKLEGPKRAESGAQVPITLTVDQNLNNPDAIKKVYVIVDANPIPLAATYHF
                     TPLNGKAQISTRIRMETDSFIHAVGETANGKLYMTAITIRAAGGCGGAVDGDDTAIRA
                     ASGKIKLSVEEPIKFGEANAATFLIKHPMFTGLQRDLASGGYKPAFYINKVAFSYGGK
                     PVMHVDFAVGVAEDPYLRFYYLPDAPGALEVKADDNEGKSFTQSVEVKS"
     gene            complement(3242..3844)
                     /locus_tag="PKW44_04005"
     CDS             complement(3242..3844)
                     /locus_tag="PKW44_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019896754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HQR50782.1"
                     /translation="MNQPIQRYTKTAIVLHWLIALLIIGMLAMGLWMAELPKDAPKVA
                     EFDLFNLGLYTVSLPEPVSVRTFYFNLHKSCGLTLLALILLRVFWRVSHQAPEFPATM
                     KAWEKKLSHAVHHSLYLLMLLVPVSGFVMSIYSKYGVAWFGTLLVNGLDNPQLRDLCK
                     EAHEFTAWLLLVIAALHIAAAIKHKMVDKDEVMRRMSLRG"
     gene            4103..4999
                     /locus_tag="PKW44_04010"
     CDS             4103..4999
                     /locus_tag="PKW44_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020183135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein relay system zinc metallohydrolase
                     2"
                     /protein_id="HQR50783.1"
                     /translation="MFIALLLCATGVCAEPLPMEEVAPGVYVHRGVHEELDTGYHGDI
                     CNIGFIVGKKGVAVIDSGGSLAVGQRLREAVRKVTPLPILYVINTHVHPDHIYGNAAF
                     LADKPAYIGHYKLADAMERRREIYTRNNAAWLGDAAAGSEMVKPTQAVQSTLELDLGE
                     RSLLLTAHPTAHTNTDLTVYDRNTSTLWSGDLLFIERTPSIDGDIKGWLQVIGKLREI
                     PAQRIIPGHGPVPASWNEALANEQRYLETLLNDVRASIRKGESMERAMDTAAAAEKDR
                     WVLFGTVNRRNVNIIYPVLEWE"
     gene            5155..5874
                     /locus_tag="PKW44_04015"
     CDS             5155..5874
                     /locus_tag="PKW44_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HQR50784.1"
                     /translation="MTSAALSGRFSDYGLAWALALSVVLHAAIVAFMQPFHFDPPPQQ
                     IALEIELQQPQPEPPPPPPPEVVKPKELPKPQPVQPRKPQPEPPPIRTPEPAPAPQAE
                     TPPPPPPPAVIAATPKPEEPPVFTAPPEPPKPVGPTPQDIDAARNHYGNLLAREFAKH
                     KQYPRVAQMRGWQGTVRVQLELDAAGKVISTSISESSGHEVLDQQAIEMVKKAIPLPL
                     PPEILRNKPLTIFVPVPFKLE"
     gene            5888..7348
                     /locus_tag="PKW44_04020"
     CDS             5888..7348
                     /locus_tag="PKW44_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQR50785.1"
                     /translation="MAHKPKSLKNLLLVHELTFIFLIVLAGMAGATGIHYWQQASHES
                     LRINQLIQEIQQTRGDLYRQMKELFDAFFLDDTQARAEYDSYTRSVEQHFRELRGLAV
                     GEEEKQAISELQLGYTDFLEETGDLLQRHSEMPSEALRKALNTDLETGVFSRFEAVST
                     RAERLLTLKQQELQQKLEDSKRNVVALMVIPLLLASLLLVFSRFFLKRAIVRPISDIL
                     YATSEISAGRLEHKAPAESRTAELAMLAGAINQMASELARSQEALVRSEKQAAQGALV
                     PMLAHNIRNPLASIRATAQVADEPGLHRETREALYDIIGTVDRLERWTGGLLAYLHPL
                     RPQPVTTSLQSIIHGALAPLQQKLREKSIDIELAPGHRNDKLSVDEQLLEQALYNLLL
                     NAVDASPASSAITIEYAIRPEQVELRILDRGPGMPFSPAPGNLSPGPSTKRFGTGLGI
                     PFAFKVCEALGGSIEFTAREGGGTSVKILLPHGPRA"
     gene            complement(7349..7906)
                     /gene="thpR"
                     /locus_tag="PKW44_04025"
     CDS             complement(7349..7906)
                     /gene="thpR"
                     /locus_tag="PKW44_04025"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HQR50786.1"
                     /translation="MKANIQRQESDPEQARIFFALWPPLAQRQQLHDLAVRYHKLCGG
                     RVMRAETLHLTLLFLGAVPMRRLQVLCNALDGLEASPFLLTLGQFCCWRHNRIGYVTT
                     TGQNAALSRLAQALREAVSMAGFSFEERAFSPHVTLLRHVEHALEPQSVVHIEWPVES
                     IALVESVVTDRGVSYRTLHRWDCQP"
     gene            7969..8424
                     /locus_tag="PKW44_04030"
     CDS             7969..8424
                     /locus_tag="PKW44_04030"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HQR50787.1"
                     /translation="MLNQAIPDFELPATGGKPFRLSQYAGVKLVLYFYPKDATPGCTN
                     EGLQFRDLHDSFRQANAEIFGVSRDSLKSHENFKAKFSFPFDLISDADELACNLFGVI
                     KIKNMYGKQVRGIERSTFVLDGNGVLRQGWRGVKVDGHAMEVLDFVNSL"
     gene            8439..9833
                     /locus_tag="PKW44_04035"
     CDS             8439..9833
                     /locus_tag="PKW44_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HQR50788.1"
                     /translation="MVKKQSPSKLFVLDTNVLMHDPSSLFRFEEHDVYLPMVTLEELD
                     NNKKGMTEVARNARQASRYIDEIIGPRPGNLEEGSSLALNSNRLSTGRLFLQTHAVSV
                     ELPMLAGSKADNQILAVVHYLQKKHPERQVILVSKDINIRIKARAMGASAEDYFNDKV
                     LEDTDLLYTGMKDLPQDFWDTHSRNMESWQDSGRMFYRVSGPLCQSLLINQFLYYEFD
                     KPFHAIVRQQEGKTAVLEVLKDYTQPKHNVWGITARNREQNFALNLLMDPDVDFITLL
                     GQAGTGKTLLTLASALTQTLDKKIYSEIIMTRVTVPVGEDIGFLPGTEEEKMAPWMGA
                     LEDNLDVLNKTDDEAGDWGRAATQDLIRTRIKIKSLNFMRGRTFLHKFLIIDEAQNLT
                     PKQMKTLITRAGPGTKVVCLGNIAQIDTPYLTEGSSGLTYVVDRFKGWEHNGHVTLMR
                     GERSRLADFAAEVL"
     gene            9865..>10140
                     /locus_tag="PKW44_04040"
     CDS             9865..>10140
                     /locus_tag="PKW44_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HQR50789.1"
                     /translation="MNQGFYTILAAQFLSALADNALLFAAIAMLARLSAPDWHTPLLQ
                     QFFVYAYIVLAPFVGPFADALPKGRGMFISNPIKFLGCLSILLGMQPL"
BASE COUNT         2289 a         3179 c         2739 g         1933 t
ORIGIN      
        1 cgcagcctgc tggccggtct ttcacttttc tgcctggcgc ccagcgcggt tgccggcacc
       61 accccggcct cgggcgagta caccagccag gaagcagagc aagccaatgt gctgctgacc
      121 tcgccagtgg tagtcaccgc cacgcgccag gaacagaaca gcttcgacct gccggtctcg
      181 atcgacgtgg tggatgccgc gacaatccac gacgccaagc tgcaggtcaa tatctccgaa
      241 gacctgccac gcgtacccgg tgtcgtcgca cagttccgcg ggcagtacgc gcaggacttg
      301 cagatttcca cccgtggttt cggcgcgcgt tcccaattcg gcgtgcgtgg cattcgcctc
      361 tacagcgacg gcatcccgct gaccatgccg gacgggcagg ggcagaccgg caccatggac
      421 ctcctcacgg cggaaagaat cgaggtgatg cgcggcccat tctccgcact gtatggcaac
      481 tcgtctggcg gcgtactgca gatttttacc gccgatggcc ccaagaatcc caccgtggat
      541 gccgcgttca tggccggcag ctatgacacc cagcgcgaag ccatcaaggt cggcggaacc
      601 gagggcgcac tcaactacaa tgccggcctt tcccatctgg aaagcgatga ctaccgcgac
      661 cacgcatcgg tacggcgcac cctgttcaat accaagcttg ggctgcaggt caacgaggat
      721 acccgcctga ccgtgctcgg cacctatctc gaccagccgg aagcacaaga cccgctgagc
      781 ctgaacaagg atcagtggga acaggacccg aagcaggccg gcatcggagc ggaaaacttc
      841 aacacgcgcg tgattcgccg ccacacccag gtcggcgcca aactggagca cgacttcgat
      901 gccgacaaca ccatccactt gatggcctac ggcggcaacc gcttcaatga gcagttccag
      961 gccgtcggcg ccaatcccag cgggaccggt atcacctctg catttctcaa gaacctgccg
     1021 ccaaacgcca acaaccagac cgccggcggg ggggcgcaga tccagcgcaa ctttggcggt
     1081 accgacttgc actacaccca taagggcttg ctcgccggca acccttacag tttcaccgtg
     1141 ggcgccaact acgacaccca gacggatgac cgcaccggtt tcgataattt ccttgccatc
     1201 cccggcactg gcaacaacag gatctgtggg gtgggtgtcg tttgcggcgt caagggaatt
     1261 ctgcgccgcg atgaggacaa caaggtctgg gactttgacc agtacgccca gggcgaatgg
     1321 tcgccacacg agcgctggaa cctgtttgcc ggggtgcgcc acagtgacgt gcacttcgag
     1381 aacgacgaca aatacctgta caacggcaac agtagcggca aggtcagcca cgaaaaaacc
     1441 acgccggtcg ccggggtgct gttcaaggtg acgcctatgc tcaacctgta cgccaatgcc
     1501 ggcaagggct tcgagacccc cacgctggtg gaaatggctt acaagccagg cggcatcggc
     1561 actttcaacc tcgacctgca gccgtccacc agcgacaatt atgaagttgg tgccaaagcc
     1621 tatctgggcg ccgatacgcg cgccaatgtt gctttcttcc acattgacac gaacgatgaa
     1681 atcgtcgttt cagacaccac caatgggcgg tcgtcctacc agaatgcggc aagtacccgg
     1741 cgccaagggg tggaagtctc gattgactcc gactttggct acggcttcac cggctacgcc
     1801 tcttacgcct atctcaatgc cgaatacagc agttcattct gcaatgcctc gactgcgtcg
     1861 aattgcgccg gggggacact ggtgcagagc ggcaaaacca tccccggcac ctacagcgac
     1921 accgcttatg ccgaactcgc ctggaaatat gcgcccagcg gtttttccac tgcgctggaa
     1981 gcacgcgcct acagccaagt ctatgtcgcc gacaccaaca acgatacagc gcccggctat
     2041 gcgctgatga gctggcgcgg cggcttcaac cagaatatca ggcaatggcg catgtcggaa
     2101 ttcgtgcggg tggacaacct gttcgaccgc gaatacgttg gctcggtcaa gctcaacgac
     2161 agcaaccagc gttattacga atccggcagc aatcgcaact ggctgctggg gctcaacgcc
     2221 agctatcagt tctgaatata gtcatcaact gcaaaacccg ccgtgtggcg ggttttgcag
     2281 tttgagagcc atagttgagt gaaccagtag gatattatcg tgtctcttat catgaagctt
     2341 tgctaatata ctaatcaaca ctcacactgg aggaaatcat gtccatctcg aaatcgctgt
     2401 cgttgctgac ggtcattagc ggcctgtgtt tagcctatag tggcagcgcc ttggccgagg
     2461 cagacgaaaa gctctggcct gcgctcaagg aaagcttttt tgccggcaag accatcgaga
     2521 acgccgactt catcaagctg gaaggcccga agcgcgccga aagcggcgcc caagtgccga
     2581 tcacgctgac ggtagatcaa aacctcaaca atcccgatgc aatcaagaag gtatatgtga
     2641 tcgtggatgc caacccgatt ccgctggccg cgacttatca ctttaccccg ctcaacggca
     2701 aggcacagat atccacccgc atccgcatgg aaaccgactc ctttatccat gccgtgggcg
     2761 aaaccgcgaa tggcaaactc tacatgaccg cgatcaccat tcgtgctgcc ggtggctgcg
     2821 gcggcgcggt ggacggcgac gataccgcga tccgtgccgc ttccggaaag atcaagctca
     2881 gcgtcgagga gccgatcaag ttcggcgaag ccaatgccgc taccttcctc atcaaacatc
     2941 caatgttcac cggcttgcag cgggacttgg catccggcgg ctacaagccg gcgttttata
     3001 tcaacaaggt ggcttttagc tatggcggca agccggtgat gcacgtcgac ttcgccgtgg
     3061 gcgtggccga ggacccctat ctgcgttttt attacctgcc ggatgcgccg ggcgcgctgg
     3121 aagtaaaagc ggacgataac gaaggaaaat cctttaccca gtcggttgaa gtcaagagct
     3181 gaaaccggga tcaccaaaga caaagggcgc aaactgcgcc ctttgtcttt tctttttgca
     3241 gttagccgcg caatgacatc cgtcgcatca cctcgtcctt gtccaccatt ttgtgcttga
     3301 tggcggcggc gatatgcagg gcggcaatga ccagcagcag ccatgccgtg aactcgtgcg
     3361 cctccttgca cagatctcgc aattgtgggt tatccagccc attcaccagc agtgtgccaa
     3421 accacgccac gccgtacttg ctgtagatcg acatcacaaa gccgctgacc ggcaccaaca
     3481 acatcagcag gtacagggaa tgatgcaccg cgtgcgacag tttcttctcc cacgccttca
     3541 tcgtcgcggg aaactctggg gcctggtggc tcacgcgcca gaacacgcgg agcaggatca
     3601 acgccagcaa ggtcaagccg caggatttgt gcagattgaa atagaaggtg cgcacagaaa
     3661 ccggctcggg cagcgagacg gtataaagcc ccaggttgaa cagatcgaac tcggccacct
     3721 tgggcgcgtc cttgggcagt tcggccatcc acaggcccat ggccagcatg ccgataatca
     3781 gcagggcgat cagccagtgc agaacaatcg cggttttggt atagcgttgt atgggttgat
     3841 tcatttgtca gcctaaacgt cacgttgaag ttttcccaca gcgcatgccg tgggaacctg
     3901 gttctcgccg ccagcggctc tggccatggt gcaaaatata cacttttttt ccttcatcgt
     3961 caggcgctgc aaccgccgcg gagtttcacc aaccagcgcc ttgatttttc gtcaacatta
     4021 actggcataa tggcccgata gtgaacactg agatttcgca accttcattc ccgatggagc
     4081 ttccatgaca cgactgcctg ccatgttcat tgccttgctg ttatgcgcta ccggtgtctg
     4141 tgccgagcca ttgcccatgg aagaagtcgc tcccggcgtc tacgtacatc gtggcgtaca
     4201 tgaggagctc gataccggct atcacggcga catctgcaat atcggattca tcgtcggcaa
     4261 gaaaggggta gccgtgatcg acagcggcgg cagtctcgcc gtcgggcagc gtttgcgcga
     4321 ggcagtgcgt aaagtgacac cactgcccat tctttatgtc atcaacaccc acgtccaccc
     4381 ggaccacatt tacggcaatg ctgcctttct tgccgacaag cccgcctata tcggccacta
     4441 caagctagcg gacgcgatgg agcgccgccg ggaaatctac acgcgcaaca atgccgcctg
     4501 gcttggcgat gccgcggccg gcagcgaaat ggtcaagcct acgcaagccg tacaatccac
     4561 gctggaacta gacctaggcg agcgctcact attgctgacg gcccacccca ccgcccacac
     4621 caacaccgac ttgactgtat acgaccgcaa cacatccacc ttgtggagcg gcgacctgtt
     4681 gttcatcgag cgcaccccct ccatcgacgg tgacatcaag ggctggctgc aagttatcgg
     4741 caagctgcgc gaaattcccg cgcagcgcat catccccggc cacggcccgg ttccagccag
     4801 ctggaacgaa gcactggcta acgagcagcg ctacctggaa accctgctca acgacgtgcg
     4861 cgccagtatc aggaaaggcg aatccatgga acgtgcaatg gacacggcag cggcagcaga
     4921 aaaagatcgc tgggtattgt tcggcaccgt caaccgtcgc aacgtcaata tcatctaccc
     4981 cgttcttgag tgggagtagc caaccgtctg ctatgcccac ttcatgcggc atccgcttgc
     5041 ggccggcatt cgccctgcgc actaccccgg aagatgtttt ccttgctgct ataatgtggc
     5101 ctttacaatc tttcgtctag ctctccacca gaccttccgc caaccgaatc cgacatgaca
     5161 agcgccgctc tctccggtcg tttcagcgac tacggactag cttgggcatt agccctgtca
     5221 gtcgtattgc atgccgccat cgtggccttc atgcagccgt tccatttcga ccccccgccc
     5281 cagcaaatag cgctggaaat cgaattgcaa cagccccaac cagagcctcc tccgccccct
     5341 cccccggaag tggtcaaacc caaggaatta cccaagccac agccggtgca gccgagaaag
     5401 cctcagccgg aaccgccacc gatcagaacg ccagagccgg cgcccgctcc gcaggctgaa
     5461 actccaccgc caccgcctcc acctgcggtc atcgcggcaa caccgaaacc agaagagccg
     5521 ccggtgttta ccgcaccgcc tgagccgccc aaacctgtcg gacccacgcc gcaggatatt
     5581 gatgctgccc gcaaccacta tggcaacctg ctggcgcgag agtttgccaa acacaagcag
     5641 tacccccgtg tcgcacagat gcgcggctgg cagggtacgg taagagtcca actagagctt
     5701 gacgctgccg gcaaggtcat ttccaccagt atcagcgaat ccagcggaca cgaggttctc
     5761 gaccagcagg cgattgaaat ggtgaaaaaa gccattcctt tgccgttgcc gccggaaata
     5821 ctgcgcaaca agccgttgac gattttcgta cccgttccat tcaagcttga atagacctta
     5881 ggccgaaatg gcgcacaaac ccaaatcgct caagaatctg ctgctagtcc acgaactcac
     5941 cttcattttc ctgatcgtgc tggcggggat ggcgggggct accggcatcc actactggca
     6001 gcaggcttcg cacgaatcgc tgcgcatcaa ccagttgatc caggaaatcc agcagacgcg
     6061 cggcgatctc taccgccaga tgaaggaatt gttcgacgcc ttctttctcg acgatacaca
     6121 ggcccgcgcc gaatacgaca gctatacccg ttccgtcgaa cagcatttcc gggaactgcg
     6181 cggcctggcc gtaggcgagg aagaaaaaca ggccatcagc gaattgcaac tgggttatac
     6241 cgactttcta gaagagactg gggacttgtt gcagcgccac agcgaaatgc ccagcgaagc
     6301 actgcgcaag gcgctcaata ccgaccttga aacaggcgtg tttagtcggt tcgaggcggt
     6361 ttcgacgcga gcggagcgac tactcacact caagcagcag gaattgcagc agaagctgga
     6421 agactccaag cgcaatgtgg tcgctttaat ggtcattccc ctcttgctag ccagtctgct
     6481 gctggtgttt tctcggtttt ttctgaaacg cgctatcgtg cgccctattt ccgacatcct
     6541 gtacgccact tctgaaatca gtgccgggcg actggaacat aaagcccccg ccgaaagcag
     6601 gaccgcagaa ctggccatgc tggccggcgc catcaaccag atggcgagcg aactggcacg
     6661 cagccaggaa gccttggtac gtagcgagaa acaggccgcg caaggcgctc tggtacccat
     6721 gctggcacac aacatccgca acccgctggc cagcatccgc gccacggcac aagtggcgga
     6781 tgaaccggga ctgcaccggg aaacccgtga agcgctgtat gacatcatcg gtaccgtgga
     6841 ccggctggaa cgctggactg gcggcctgct cgcctacctg catccgctca ggccgcaacc
     6901 ggtcaccacc agcctgcaat ccattatcca cggcgcgctg gcgccgttgc agcaaaagtt
     6961 gcgtgagaaa tccatagaca tcgagctcgc acccggccac cgcaacgaca agctgagcgt
     7021 ggatgaacaa ttgctggaac aggcactata taacctgttg ctgaacgccg ttgacgcttc
     7081 acccgccagc tcagccatca ccatcgaata cgccatccgg ccggagcagg tggaattgcg
     7141 catcctggat cgcggccccg gcatgccgtt ttcccccgcg cccggcaatc tcagtccggg
     7201 cccttccacc aagcgcttcg gcaccggact cggcattcca tttgccttca aggtttgcga
     7261 ggcactgggc ggcagcatcg aattcaccgc ccgcgagggg ggtggaacgt ccgtcaagat
     7321 cctcctgccg cacgggccaa gggcctgatc atggctggca atcccagcga tgcagcgttc
     7381 ggtagctgac cccgcggtcg gtcaccaccg actccactag cgcaatcgac tcgaccggcc
     7441 attcgatgtg cacgaccgac tgcggttcga gcgcatgctc gacatgacgc agcaaagtca
     7501 cgtgcggtga aaaagcgcgc tcttcgaagc tgaatccggc catggatact gcttctcgca
     7561 acgcttgcgc aagtcgggac agggcggcat tctggcctgt cgtcgtaacg taaccgatgc
     7621 gattgtgccg ccagcagcaa aactgtccca gcgtcaacaa gaacgggctg gcctccagcc
     7681 catccagcgc attgcataac acctgcagcc ggcgcatggg taccgcccca agaaataaca
     7741 gggtcaggtg cagcgtttca gcgcgcatca cccggccgcc gcaaagtttg tggtagcgca
     7801 cggccagatc atgcaattgt tgccgttgcg caagcggcgg ccacagtgca aaaaatatcc
     7861 tagcctgctc agggtcgctt tcctgtcgct gaatattcgc cttcatctat tgccttaccc
     7921 gcgtttttta acaacaatgg tgccgtaaag acgttagact ctcacaacat gttgaatcag
     7981 gccatccccg acttcgagct tcctgccacc ggcggcaaac cattcaggct ttcgcagtat
     8041 gccggagtca agctggtact ttatttttac cccaaggatg ctacgccggg gtgcactaac
     8101 gagggactgc agttccgtga cctgcatgac agtttcaggc aggcgaacgc ggagatcttc
     8161 ggcgtttccc gcgatagcct gaaatcgcat gaaaacttca aggctaaatt cagtttcccg
     8221 ttcgacctga tttcagatgc tgatgagttg gcttgcaatc tgttcggcgt catcaaaata
     8281 aaaaacatgt atggcaagca ggtgcgcggt atcgagcgca gcacgtttgt gctcgatggc
     8341 aatggcgtat tgcgccaggg atggcgtggc gtcaaggtcg atggccacgc tatggaagtt
     8401 ctcgatttcg tcaattcact ctaggaaact cgtccggcat ggttaaaaaa caaagcccca
     8461 gcaaactgtt cgtgctcgac accaacgtcc tgatgcacga cccctccagc ctgttccgct
     8521 tcgaggagca cgatgtctac ctgcccatgg tgacactgga agaactggac aacaacaaga
     8581 agggcatgac ggaagtcgcg cgcaacgccc gccaggccag ccgctatatc gacgaaatca
     8641 tcggcccgcg accgggcaat ctggaggaag gcagctcgtt ggcgctcaac agcaaccgcc
     8701 tcagcaccgg ccgcctgttc ctgcagaccc acgcggtcag cgtcgaattg cccatgctgg
     8761 cgggtagcaa ggcggacaat cagatcctgg cggtggtgca ttacctgcag aaaaaacacc
     8821 cggaacgcca ggtcatccta gtcagcaagg acatcaacat ccgcattaag gcacgcgcca
     8881 tgggggcgtc ggcggaagat tatttcaacg acaaggtgct ggaggatacc gaccttctct
     8941 acaccggcat gaaggatctg ccacaggatt tctgggacac ccacagcagg aacatggagt
     9001 catggcagga ttccgggcgc atgttttacc gcgtctccgg ccctttgtgc cagagcctgc
     9061 tgatcaacca gtttctctat tacgaattcg acaagccttt tcacgccatc gtacgccagc
     9121 aggaaggcaa gacggcggtg ctggaggtgc tcaaggatta tacccagccc aaacacaacg
     9181 tctggggcat cactgcgcgc aaccgcgaac agaatttcgc cctcaacctg ctgatggatc
     9241 ctgacgtgga tttcatcacc ctcctaggcc aggccggcac cggcaagacc ttgctcacac
     9301 tggcctccgc cctcacccag accctggaca agaaaatcta cagtgaaatc atcatgaccc
     9361 gtgtgactgt gccggtaggc gaggacatcg gtttcctgcc cggcaccgaa gaggaaaaga
     9421 tggcgccatg gatgggcgcg ctggaagaca atctggacgt gctcaacaag acggatgacg
     9481 aggctggcga ttggggacgt gccgcaaccc aggatttgat ccgcacccgc atcaagatca
     9541 agtcgctcaa cttcatgcgc ggccgcactt tcctgcacaa gttcctcatc atcgacgagg
     9601 cacagaacct gacgcccaaa cagatgaaaa ccctgattac ccgtgccgga ccgggaacca
     9661 aggtcgtctg cctgggcaat atcgcgcaga tcgacacccc ttatctgacc gaaggcagct
     9721 ccggcctgac ctatgttgtc gatcgcttca agggctggga acacaacggc cacgtcaccc
     9781 tgatgcgcgg ggagcgctcc aggctggccg atttcgccgc tgaagtactc tagctcgtta
     9841 atgaccacgc gaaccgcagg taccataaac cagggcttct ataccattct ggcggcacag
     9901 ttcctgtcgg cgctggccga caacgcactg ctgtttgccg ccattgcgat gctggcacgg
     9961 ctgagtgcgc cagactggca tacgccgctg ttgcagcagt tttttgtcta tgcctatatc
    10021 gtgctcgccc cttttgtcgg ccccttcgcc gatgcgctgc ccaaagggcg ggggatgttc
    10081 atctccaatc ccatcaaatt tctgggctgc ctttccattc tgctcggcat gcaaccgctg
//
LOCUS       DAOMFA010000053        10065 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_718_length_10065_cov_4.642158, whole genome shotgun sequence.
ACCESSION   DAOMFA010000053 DAOMFA010000000
VERSION     DAOMFA010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 10065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10065
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_718_length_10065_cov_4.642158"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1529)
                     /locus_tag="PKW44_04045"
     CDS             complement(<1..1529)
                     /locus_tag="PKW44_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013146734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HQR50790.1"
                     /translation="MQSQSAQAAFPPSYRLGIGAYDEMLDGAGNVRPHWGQLMHALHA
                     LGPQEVEQRHKEALKLLRENGVTYNVYGDPDGANHPWQLDPVPLLISGKEWYDIEAGL
                     LQRAELLNLLLADLYGPRELIRKGLLPLELVYNHGGFLRACDQVRLPGRHQLILYAAD
                     LARGPDQNMWVLGDRTQAPSGTGYALENRLAMSRVLPSLFRSAQVHRLEPFFQSLRAG
                     LNSLSPQQSDIPRVVVLTPGPFNETFFEHAYLASHLGYPLVQGDDLTVRDGHVWLKSL
                     TGLQRVDIILRRVDDNYCDPLELRGDSRLGVPGLLEAARRGNVAIANPLGSSILENPG
                     LQPFLPGIARHFLGQPLQLPSIATWWCGQPQEMDYVLANLDQLVIKPIYRNPGVRSVF
                     GHLLSQQQLAEWRDRIRANPAFYVGQEHESFSTVPSVVAGGYEPRQALLRCFLVARAD
                     GYTVMPGGLTRSAPEKGDMPISNQMGGISKDTWIIASEPQHVTTNIKPEHAADSHGNH
                     N"
     gene            complement(1549..4311)
                     /gene="ppc"
                     /locus_tag="PKW44_04050"
     CDS             complement(1549..4311)
                     /gene="ppc"
                     /locus_tag="PKW44_04050"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013146738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HQR50791.1"
                     /translation="MRPSTPSPAQALKENIRHLGRILGEVIKDKEGEATFQTIEDIRQ
                     AAVKFHRDGDPASAATLDRLLKGLDTDQSIAVARAFSYFKHLVNIAEDLASHQQIAHG
                     EAASEPGQLACSLDNFQREHMTLEEIGTFFAAALISPVLTAHPTEVQRKSLLDTQRSI
                     STLLARQSLPLPRHEAERNARMLYAAITLLWQSRMLRFSRLTVNDEIDNALSYYGMSL
                     LDTIPELLQDLELDVGSRFGAGGEPPHFPSFFQMGSWIGGDRDGNPNVNAATLEQAIA
                     RQAATVLDYYLRETDALRAELSLTTRLVDVSPKLKKLADHSPDQSPHRQDEPYRLALV
                     AVLERLRQSCKALTGKKTPRNDDALPAYEDSEAFLADLDIIADSLIDHAGQALVYPRL
                     GKLIKAVETFGFHLASIDLRQSSDVHENVITELFIKAGEDFSYNELGEAEKVKVLLAE
                     LKQPRLLYSPYQDYSALVESEIGVFRKAKEVREKFGPRAVRQYIISHTEALSDLLEVA
                     LLQKEAGLLRGRWGSSRVQMDLNIAPLFETIEDLRNAPRIMSEWLSLLGIRHLLKYQG
                     SLQEIMLGYSDSNKDGGFLTSNWELYKAEVSLVELFQQAKIRLRLFHGRGGTVGRGGG
                     PTYQAIMAQPQGTVDGQIRLTEQGEIIANKFSDPLVARRNLETLIAATIDATLFPQDQ
                     LAPARRRTYEILMDELSATAMAEYRNLVYETPGFADYFFSATPISEIAELNIGSRPSS
                     RKATQRIEDLRAIPWGFSWGQCRLLLPGWYGFGSAIARYLSIDREQRTQTLREMLKQW
                     PLFKTLLSNMDMVLAKIDLAVASRYAQLVEDATLRDQVFQRIVREHELTTQALDLLLE
                     SPVRLASNPALAESIKRRLPYLDPLNHLQVEMIRRHRSGATDERVKRNIHLTINGIAA
                     GLRNTG"
     gene            4452..5180
                     /locus_tag="PKW44_04055"
     CDS             4452..5180
                     /locus_tag="PKW44_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012777549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome-type protease"
                     /protein_id="HQR50792.1"
                     /translation="MTYCVGLLLDAGLVMASDSRTNAGVDHVATFPKMTIFSRPDERV
                     LVLLTSGNLAVSQAVVSRLNKDIAEGMEPNLHSATCMFEAARIVGAAVRAVHHEDAEH
                     LKKHQADFNQAILLGGQIRGEKPRLFSIYAAGNFIEAVAETPYFQIGETKYGKPILDR
                     VINSGGDLNEAAKCTLISFDSTIRSNLSVGMPIDLFMYRTDSYSPDCQIRITDSDPYF
                     NQLRKGWGEGLKKVFASLPDPDWC"
     gene            5267..7159
                     /gene="htpG"
                     /locus_tag="PKW44_04060"
     CDS             5267..7159
                     /gene="htpG"
                     /locus_tag="PKW44_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HQR50793.1"
                     /translation="MTTETVQKETLGFQAEVKQLLHLMIHSLYSNKEIFLRELISNAS
                     DAADKLRFEALSDGALFENDSELKIRLAFDKEARTLTIADNGIGMSRQEVIDNIGTIA
                     KSGTREFFAQLSGDQKKDAALIGQFGVGFYSAFIVADKVTLTTRRAGLTAEHGVRWES
                     GGEGDYTLETVEKPTRGTEIVLHLREGDDEFLSDWKLKSTIRKYSDHITLPIVMKKSE
                     WKDGAEVPTDEDETVNQASALWARSKNDITDEQYQEFYKHVAHDFELPLAWSHNRVEG
                     KQEYISLLYVPGHAPFDLYDRERRHGIKLYVKRVFIMDDAEQLMPQYLRFVRGVIDSA
                     DLPLNVSREILQHSKEIDGIKAGSVKKVLGLLEDLAENAPEKYAGFWKEFGRVLKEGP
                     GEDFANREKIAGLLRFATTHADSEEQTVSLKDYIGRMKDGQDKIYYVTADSFAAARHS
                     PHLEIFRKKGIEVLLLSDRVDEWLVGSLLEFDGRKLQSVAKGDLDLGKLEDEAEKEQQ
                     KKAEDAFKPLVERVQATLGDKVKEVRVTHRLTDSPACIVTGEHDMSANLERLLKAAGQ
                     QAPNTRPTLEINPTHALVARLQNEADDARFADWAHLLFDQALLAEGGQLDDPASFVKR
                     LNSLLV"
     gene            complement(7454..8569)
                     /locus_tag="PKW44_04065"
     CDS             complement(7454..8569)
                     /locus_tag="PKW44_04065"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HQR50794.1"
                     /translation="MSLHQTPATDDVRILEIRELTPPLEILEKLPASRTASANILATR
                     TAIHRILQGTDDRLLVVVGPCSIHDCAAGLEYAKRLAEQRDRFRKELLVVMRVYFEKP
                     RTTIGWKGLINDPHLDGSFQINVGLELARRFLLEVNELGVPAGSEFLDTISPQYTADL
                     VSWGAIGARTTESQVHRELASGLSCAIGFKNGTDGNIHIAADAIKAAANPHHFLGATK
                     DGRSAIISTAGNGDCHLILRGGKTPNFDTASINAACEILIAAGLAPRAMIDCSHGNSH
                     KQYKMQMDVARNIAGQLGNDERRIMGVMVESHLHEGRQDYKPGGMLEYGKSITDACLG
                     WEDTVALLEQLASSVHARRRKFQNTTPSFMKIPSRDS"
     gene            8896..9375
                     /gene="bfr"
                     /locus_tag="PKW44_04070"
     CDS             8896..9375
                     /gene="bfr"
                     /locus_tag="PKW44_04070"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HQR50795.1"
                     /translation="MKGDRKIIEILNGLLAGELAAVDQYLIHGEMYADFGFPHLSEKS
                     LHESEHERQHARALIQRILFLEGKPNMVKRTVLNIGSTVPEMLKADLAVEYHVVGELK
                     KAIAACEKAQDYVTRDMLGVQLEDTEMDHAYWLEQQIRLIGLVGLANYQQSQMKPGQ"
     gene            9389..9871
                     /gene="bfr"
                     /locus_tag="PKW44_04075"
     CDS             9389..9871
                     /gene="bfr"
                     /locus_tag="PKW44_04075"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HQR50796.1"
                     /translation="MKGDKKVIDILNKVLKNELTAINQFFLHARMFRNWGLEKLNDYH
                     YKQSIRVMKEADEVIERVLFLEGLPNLQNLGKLLIGENVVECLQGDLRYEKEMQRPLL
                     VEAIALCEAHQDYVSRDLLEELLEHCEEAIDWLETQRTLIQDVTLPNYLQSHMEPSED
                     "
BASE COUNT         1954 a         2852 c         3185 g         2074 t
ORIGIN      
        1 ttgttgtggt tgccgtggct gtcggcggca tgttccggct tgatgttggt ggtgacatgc
       61 tgcggctcgg aggcgatgat ccaggtgtcc ttgctgatgc ctcccatctg gttggagatc
      121 ggcatgtcgc ctttttccgg cgcgctgcgg gtgaggccgc ccggcattac cgtgtagccg
      181 tcggcgcgcg ccaccaggaa gcagcgcagc aacgcctggc gcggctcata gccgcctgcc
      241 accaccgagg gcacggtgga aaagctttcg tgctcctgcc cgacgtaaaa cgccgggttg
      301 gcacggatgc ggtcgcgcca ttcggccagt tgctgctggc tgagcagatg gccgaatacc
      361 gagcgtacgc cgggattgcg gtaaatcggc ttgatgacca gctggtcgag attggccagc
      421 acgtagtcca tttcctgcgg ctggccgcac caccaggtgg cgatggaagg cagttgcagc
      481 ggctggccga ggaaatgccg ggcgatgccg ggcaggaacg gctgcaggcc ggggttttcc
      541 aggatgctgc tgccgagcgg gttggcgatg gcgacgttgc cgcgtcgcgc cgcctccagc
      601 aggccgggca cacccaggcg cgaatccccg cgcagttcca gcgggtcgca gtagttgtcg
      661 tccacgcggc gcagaatgat atccacgcgt tgcaggccgg tgagcgattt cagccagacg
      721 tggccgtcgc gcactgtcag gtcatcgccc tgaaccagag ggtagccgag gtgggaagcc
      781 aggtaggcgt gctcgaaaaa ggtctcattg aacggccccg gcgtcagtac caccacgcgc
      841 gggatgtcgc tttgctgcgg agacaggcta ttcagtcctg cacgcagcga ctggaagaaa
      901 ggctccagtc ggtgcacctg cgcgctgcgg aacaggctgg gcagcacacg cgacatcgcc
      961 aggcggtttt ccagagcgta accagtgccg gaaggtgcct gggtgcggtc gcccagcacc
     1021 cacatgttct ggtccgggcc gcgcgccagg tcggcggcat aaagaatcag ctgatgccgt
     1081 ccgggcagcc tgacctgatc gcaggcgcgc aggaagccgc cgtggttgta gaccagttcc
     1141 agcggcagca ggcccttgcg tatcagctcg cgcgggccgt aaaggtcggc cagcaggagg
     1201 ttgagcagtt cggcacgctg caatagtccg gcttcgatgt cgtaccactc tttgccggaa
     1261 atcagcagtg gcaccgggtc gagctgccag ggatggttgg cgccgtccgg gtcgccatag
     1321 acgttgtagg tgacgccgtt ttcccgcagc agcttgagcg cttccttgtg gcgctgttcg
     1381 acctcctgcg ggccaagtgc atgcagggcg tgcatcagct gcccccagtg cggacgcaca
     1441 tttccagcgc cgtccagcat ctcgtcgtag gcgccgatgc cgagccggta ggaagggggg
     1501 aacgccgcct gtgccgattg ggattgcatg agggcagttt accccgagtt aacccgtatt
     1561 gcgcaatccg gcagcgatgc cgttgatggt caggtgaata ttgcgcttga cgcgttcgtc
     1621 cgtggcgccg gaacgatggc gccggatcat ctccacctgc aggtggttga gcgggtcgag
     1681 atagggcagg cggcgcttga tcgattcggc cagcgccggg ttgctggcca gcctgaccgg
     1741 gctttccagc agcaggtcga gcgcctgcgt ggtgagctcg tgttcgcgaa cgatgcgctg
     1801 gaacacctgg tcgcgcagcg tggcatcttc gaccagttgt gcgtagcgcg aggcgacggc
     1861 gagatcgatc ttggccagca ccatgtccat gttcgatagc agggtcttga acagcggcca
     1921 ttgcttgagc atctcgcgca gggtttgtgt gcgctgctcc cggtcgatac tcagatagcg
     1981 cgcgatggcg ctgccaaagc cgtaccagcc tggcagcaac agccggcact gcccccagga
     2041 aaagccccag gggatggcgc gcaggtcctc gatgcgctgc gtggccttgc gcgaggacgg
     2101 ccggctgccg atattgagct cggcaatttc gctgatcggc gtggcgctga aaaagtagtc
     2161 ggcaaagccc ggtgtttcgt ataccaggtt gcggtactcg gccatggcgg tggccgagag
     2221 ctcgtccatg agtatttcgt aagtgcggcg tcgggctggc gcgagctgat cctgcgggaa
     2281 cagcgtggcg tcgatggtgg cggcgatcag tgtttccaag ttgcggcgcg cgacgagcgg
     2341 gtcggagaac ttgttggcga tgatttcgcc ctgttcggtc aggcggatct ggccgtccac
     2401 cgtgccttgc ggctgtgcca tgatggcctg gtaggtcggc ccgccaccac ggccgacggt
     2461 gccgccacgg ccgtggaaca gacgcagcct gatcttggcc tgctggaaca attccaccag
     2521 cgacacctcg gccttgtaca gttcccagtt ggaggtgagg aaaccgccat ccttgttgct
     2581 gtcggaatag cccagcatga tttcctgcaa actgccctgg tacttcagca gatggcggat
     2641 gccgagcagg ctcagccatt cgctcatgat gcgcggcgcg ttgcgcaggt cttcgatggt
     2701 ctcgaacaac ggcgcgatgt tgaggtccat ctgcaccctg gaagaacccc agcgtccgcg
     2761 cagcaggccc gcttccttct gcagcagcgc cacttccagc aggtcggaca gggcctcggt
     2821 gtgcgagatg atgtactgac gtactgcacg cgggccgaat ttctcccgga cttccttggc
     2881 cttgcggaat acgccgattt cgctttccac cagcgcggaa tagtcttggt acggcgaata
     2941 gagaaggcgc ggctgcttca attcagccag caataccttc actttttccg cttcgcccag
     3001 ctcgttgtag ctgaagtcct cgccggcttt gataaacagc tcggtgatga cgttctcgtg
     3061 tacgtccgag ctttgccgca ggtcgatgct ggcgaggtgg aagccgaagg tctcgaccgc
     3121 cttgatgagc ttgcccagcc gcggatacac cagcgcctgg cctgcgtggt cgatcagcga
     3181 atcggcgatg atgtcgaggt cggcgaggaa tgcctcgctg tcttcgtagg cgggcagggc
     3241 atcgtcattg cgcggggttt ttttgccggt gagcgccttg cagctttgtc gcaggcgttc
     3301 cagcaccgct accagcgcca gccgataggg ctcgtcctgg cggtggggtg actggtcggg
     3361 cgagtggtcg gccagtttct tgagtttggg cgagacgtcg accagccgcg tagtgagcga
     3421 aagctcggcg cgcagcgcgt cggtttcccg caggtaatag tcgagcacgg tggcggcctg
     3481 ccgtgcgatg gcctgttcca gcgtggcggc gttgacgttg gggttgccgt cgcggtcgcc
     3541 gccgatccag ctgcccatct ggaaaaagct gggaaaatgg ggcggctcgc cgcccgcgcc
     3601 gaagcgactg ccgacgtcca gttccaggtc ctgcagcagt tccggaatgg tatccagcag
     3661 gctcatgccg tagtaggaga gggcgttgtc gatttcgtcg ttcacggtca ggcgcgaaaa
     3721 acgcagcatg cggctctgcc acagcagggt gatggcggca tacagcatgc gcgcgttgcg
     3781 ctcggcctcg tggcgtggca gcggcaggga ttgcctggcg agcagggtgg agatgctgcg
     3841 ctgggtgtcg agcaggctct tgcgctgcac ctcggtgggg tgggcggtca gcactggcga
     3901 aatcagggcg gcagcgaaaa acgtcccgat ctcttccagc gtcatgtgtt cgcgctggaa
     3961 attgtcgagc gagcaggcca gttgaccggg ttcggatgcg gcctcgccgt gcgcaatttg
     4021 ctggtggctg gccagatcct cggcgatatt gaccagatgc ttgaagtagc tgaaggcgcg
     4081 tgccacggcg atggactggt cagtatccag gcctttcagc aggcggtcga gcgtggcggc
     4141 cgaggccggg tcgccatcac ggtggaactt cactgcagct tggcggatgt cttcgatggt
     4201 ctggaaggtg gcttcgccct ccttgtcctt gataacttcg cccaggatgc ggccgagatg
     4261 gcggatgttt tccttgagcg cctgtgcggg cgacggtgtg cttgggcgca tgtgtggact
     4321 cctcgattgc cggctggctg gatattcttc ggcattatcg aagcaactat caagccagtc
     4381 agtcaagcgg ttgtttaagc cagttctggc ggctatgatg gcaggcatta caactttacc
     4441 ccacttttat catgacttat tgtgtaggtt tgctgctcga cgccggcctt gtcatggcct
     4501 ccgattcccg caccaatgcg ggggtggacc atgtggccac gttccccaag atgacgattt
     4561 tctcccggcc tgacgagcgg gtgctggtgc tgctgacttc cggcaacctg gcggtttccc
     4621 aggcggtggt gagccgcctg aacaaggata ttgccgaagg gatggagccc aacctgcaca
     4681 gcgcgacctg catgttcgag gccgcacgaa ttgtgggcgc tgcggtgcgt gccgtgcacc
     4741 atgaagatgc ggagcatctc aagaagcacc aggccgactt caaccaggcc atcctgcttg
     4801 gcggccagat caggggcgaa aagccgcgcc tgttcagcat ttacgcagcc ggcaacttca
     4861 tcgaggcggt ggccgaaacg ccttacttcc agatcggcga aaccaaatac ggcaagccca
     4921 ttctcgaccg cgtcatcaac agcggtggcg acctcaacga ggccgccaag tgcacgctga
     4981 tttccttcga ctccaccatc cgcagcaacc tgtcggtcgg catgccgatc gacctgttca
     5041 tgtaccgtac cgacagctac agccccgact gccagatccg catcaccgac agcgacccct
     5101 acttcaacca gttgcgcaaa ggctggggcg aagggctgaa aaaggtgttc gcgtcgctgc
     5161 ccgatcctga ctggtgctga tttcctgttg ccgggggctt gaattcccga ggtgcgcccc
     5221 catacatact cccgttggtg ttcattagaa attctgggag aactgcatga caaccgaaac
     5281 cgtacaaaaa gaaaccctgg gctttcaggc cgaggtcaag caactgctgc acctgatgat
     5341 ccactccttg tattccaaca aggagatttt cctgcgcgag ctgatttcta acgcctccga
     5401 tgccgccgac aagctgcgct tcgaggcgct gtccgatggc gcgctgttcg agaacgacag
     5461 cgagctgaaa atccgccttg ctttcgacaa ggaagcacgc acactgacca ttgccgacaa
     5521 cggcatcggc atgagccggc aggaagtcat cgacaacatt ggcaccatcg ccaaatccgg
     5581 cacgcgcgag ttcttcgccc agctttccgg cgaccagaag aaggatgcgg cgctgatcgg
     5641 ccagttcggc gtgggcttct attctgcctt cattgtcgcc gacaaggtga ccttgaccac
     5701 ccgccgtgcc ggcctcaccg ccgagcatgg cgtgcgctgg gaatcgggcg gcgagggcga
     5761 ctacacgcta gaaacggtgg agaagccgac gcgcggcacc gaaatcgtgc tgcatctgcg
     5821 cgagggcgat gacgagttcc tcagcgactg gaagctcaag tccaccatcc gcaaatactc
     5881 cgaccacatc accctgccca tcgtgatgaa gaagtccgag tggaaggacg gcgcagaggt
     5941 gccgactgac gaggacgaaa cggtcaatca ggcctctgcc ctatgggcaa ggtcgaagaa
     6001 cgacatcacc gacgaacagt accaggagtt ctacaagcac gtcgcgcacg atttcgagtt
     6061 accgctggcc tggtcgcaca accgcgtcga aggcaagcag gaatacatct cgctgctgta
     6121 tgttccgggc cacgcgccgt tcgatctgta cgaccgcgaa cgcaggcatg ggatcaaatt
     6181 gtatgtgaag cgcgtgttca tcatggacga tgccgagcag ctgatgccgc agtatctgcg
     6241 cttcgtgcgc ggggtgatcg actccgctga tctgcccttg aacgtgtcgc gcgaaatcct
     6301 ccagcacagc aaggaaatcg atggcatcaa ggccggatcg gtaaagaagg tgctgggcct
     6361 gctggaagat ctggccgaaa atgcgccgga aaaatatgcc gggttctgga aggaatttgg
     6421 ccgcgtgctg aaggaagggc cgggcgagga cttcgccaac cgcgaaaaga ttgccggctt
     6481 gctgcgcttc gccaccaccc atgccgacag cgaggagcag accgtctcgc tcaaggacta
     6541 catcggccgc atgaaagacg gccaggacaa gatctactac gtcactgccg acagcttcgc
     6601 cgccgccagg cacagcccgc acctggaaat tttccgcaaa aagggcatcg aggtgctgct
     6661 gctgtccgac cgcgtggatg aatggctggt gggcagcctg ctggaattcg acggcaggaa
     6721 gctgcagtcc gtggccaagg gcgaccttga cctcggcaag ctggaagacg aagccgagaa
     6781 ggaacagcag aagaaggccg aggacgcgtt caagccgctg gtcgagcgcg tccaggccac
     6841 gctgggcgac aaggtgaagg aggtgcgcgt gacgcaccgc ctgactgatt ccccggcttg
     6901 catcgttact ggcgagcacg acatgagcgc caacctggaa cgcctgctca aagctgccgg
     6961 ccagcaggcg ccgaacacca ggccgacact ggaaatcaat cccacccacg ccttggtggc
     7021 gcgtctgcaa aacgaagccg acgatgcccg ttttgccgac tgggcgcatt tgctgttcga
     7081 ccaggcgctg ttggccgagg gcggccaact ggatgacccg gccagtttcg tcaagcgcct
     7141 caattccctg ctggtgtaga tgcaccgatt cgatacgtgg caacaaaaac ggcagcctcg
     7201 gcttggtcca tcgattaagg cgttgccagg tgattgaggg tgcaaatgat taagcccctg
     7261 atttcttgcg aagtcagggg catttatttt gtcccggcat cagttcggga cgacttttat
     7321 cattcggaga aattgcgcca ctgacgtagt tttgcggtaa tactgacagg tggcatttat
     7381 cggccaaaag ctgcctttgc taatttaatt gcagagtgca tctaacaatc atgaaattac
     7441 ttactcgtac aagtcatgaa tcgcggctag ggattttcat gaatgatggg gtggtgttct
     7501 ggaatttccg gcgccgcgca tgaacgctgc tcgccagttg ctccaacagc gcaaccgtat
     7561 cttcccagcc taaacaggca tcggtaatcg acttcccgta ttccagcatg ccgcctggtt
     7621 tgtaatcctg gcggccttca tgcaagtggg attccaccat cacccccatg atacggcgct
     7681 cgtcattgcc tagctgtccg gctatattcc tggccacgtc catctgcatt ttgtactgct
     7741 tgtggctgtt gccatgtgag cagtcgatca ttgcccgggg cgccagtccc gctgcgatga
     7801 ggatttcgca ggcggcattg atgctggccg tgtcgaagtt cggtgttttg ccaccgcgca
     7861 gtatcaggtg gcaatccccg ttgccggcgg tggagatgat ggcggagcgc ccatccttgg
     7921 tcgcgccgag aaaatgatgc gggttggccg ccgccttgat ggcgtcggcg gcgatgtgga
     7981 tgttgccgtc cgtgccgttc ttgaatccta tggcgcaaga caggcctgat gccagttcgc
     8041 gatgaacctg cgattcggtc gtgcgcgcgc ctatggcgcc ccagctcacc aggtcggccg
     8101 tatattgcgg cgagatggta tcgaggaatt cgctgcccgc cggcacgccg agttcgttga
     8161 cttccagcag aaagcgcctg gccagttcaa gcccgacgtt gatctggaag ctgccgtcca
     8221 gatgcggatc gttgatcagg cctttccagc cgatggtcgt gcgtggtttt tcaaaataga
     8281 cgcgcatgac aaccagcaat tccttgcgga agcggtcgcg ctgttcggcc aggcgcttgg
     8341 cgtactccag gccggcggca caatcgtgga tggagcatgg cccgacgacg accagcaggc
     8401 ggtcatccgt gccttgcaga atgcggtgga tggcggtgcg cgttgccagg atgttggcgc
     8461 tggcggtgcg gctggccggg agtttttcca gaatttctaa cggtggggtc agttcccgga
     8521 tttccaggat gcgaacgtcg tctgtcgcgg gtgtctggtg cagactcatg ggtatgcgga
     8581 gacttcctcg ttacgttggt ttcttacaat gtagagcgac attttcatca ccttcatcat
     8641 ttctgcaagt tgtcagccag catccgggct gtacattgga ttagacagcc ctgtatccag
     8701 ccattccgct cgggcaggcg tggcagccat gagtttctat taagtaaatt catgaattgg
     8761 atatgcgtat atccagggtg cgactctccc gtcagaactc atgcaaatca tgcgcccgtt
     8821 gtgcgctctt ggggtatgat ttcttcatga tgatggtagt ctgcgtgttc cgatatccat
     8881 tctaaggaga aagccatgaa aggtgacagg aagatcattg aaattctcaa cggcctgctg
     8941 gccggtgaac ttgcggcggt ggatcaatac ctgatccatg gcgaaatgta tgcggatttc
     9001 ggttttccgc atctttcgga aaagtcgttg catgagtccg agcatgagcg gcaacatgcg
     9061 cgcgcgctga ttcagcgcat cctgtttctg gaaggcaagc ccaatatggt caagcgcact
     9121 gtgcttaata ttggcagtac tgtccccgaa atgctcaagg ccgatctggc ggttgaatac
     9181 catgtcgtcg gcgaactgaa aaaagccatt gcggcctgtg aaaaagccca ggactatgtc
     9241 acgcgcgaca tgctgggggt gcagctggaa gataccgaaa tggatcatgc ttactggctg
     9301 gagcagcaga tccgcctgat tggcctggtg gggctggcga attaccagca aagccagatg
     9361 aaacctggac aatagaggag gggtggccat gaagggcgac aagaaagtca tcgatattct
     9421 caacaaggta ctcaagaacg agttgactgc catcaaccag tttttcctgc acgcgcgcat
     9481 gttccgcaac tggggactgg aaaagctcaa cgactaccat tacaagcagt ccatccgcgt
     9541 gatgaaggag gcggacgagg tgatcgagcg tgtcctgttc cttgaaggac tgcccaacct
     9601 gcaaaacctc ggcaagctgc tgatcggcga aaacgtggtg gagtgcctgc agggagacct
     9661 caggtatgaa aaggaaatgc agcgtccatt gctggtggag gccatcgctc tctgcgaagc
     9721 ccatcaggat tacgtcagcc gtgacctgct ggaagagttg ctggagcatt gcgaggaggc
     9781 catcgactgg ctggaaacgc agcgaacgct gattcaggac gtgaccttgc ccaattatct
     9841 gcaatcccac atggagccca gcgaggactg attcccgtcg cccgagcgat gcctttttga
     9901 aagccgcgtc ctaacgcggc ttttttattg tggctgttgc ggcccaagct tcccggaatg
     9961 tgagcgtgcg cataatgctt gacacaagat tgaatgtgaa tgataatgat tcctatatag
    10021 attattaaca gattcatttc caggagtttg ctgcaatgta tgtct
//
LOCUS       DAOMFA010000054         9857 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_740_length_9857_cov_5.210875, whole genome shotgun sequence.
ACCESSION   DAOMFA010000054 DAOMFA010000000
VERSION     DAOMFA010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 9857)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9857)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9857
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_740_length_9857_cov_5.210875"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..616
                     /gene="earP"
                     /locus_tag="PKW44_04080"
     CDS             <1..616
                     /gene="earP"
                     /locus_tag="PKW44_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor P maturation arginine
                     rhamnosyltransferase EarP"
                     /protein_id="HQR50797.1"
                     /translation="LWQALGLLAPAEDEIAVSLFCYDTAPLPALLDAWAAAARPVRCL
                     VPEGKALSKVAACFGRSSLTPGDTLQRGNLTLHVLPFMPQEDYDLLLWACDCNFVRGE
                     DSFVRAQWAAKPMVWHIYPQQENAHHIKLDAFLDLYCSDLAEDAATTLRAFHHGWNNQ
                     GPLCWDDFRNHHTALQAHAAAWAGRLAEQSDLASKLVIFCKNRV"
     gene            656..1213
                     /gene="efp"
                     /locus_tag="PKW44_04085"
     CDS             656..1213
                     /gene="efp"
                     /locus_tag="PKW44_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HQR50798.1"
                     /translation="MKTAQELRVGNVFMVGSDPMVVLKAEYSKSGRNSSVVKMKFKNL
                     LTESPSEAVYKADDKFDVVVLEKKDVTYSYFADPMYVFMDAEYNQYEVEAETMSDALN
                     FLQDGMPCEVVFYNGKAISVELPNSVVREITYTEPAVKGDTSGKVMKPARTATGFELP
                     VPLFCEIGDKIEIDTRTLEYRNRVK"
     gene            1314..1649
                     /locus_tag="PKW44_04090"
     CDS             1314..1649
                     /locus_tag="PKW44_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HQR50799.1"
                     /translation="MDNKTAVGLLTSLAQESRLSLFRLLVQAGPSGLAAGSIAEQLGV
                     PASTLSFHLKELSHARLVQARQDGRFIYYSADFAVMNALLGFLLENCCGGQQDCCSPI
                     IVTSKGSPS"
     gene            1646..2113
                     /locus_tag="PKW44_04095"
     CDS             1646..2113
                     /locus_tag="PKW44_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020203138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsI/CadI family heavy metal resistance
                     metalloenzyme"
                     /protein_id="HQR50800.1"
                     /translation="MKRMHIHVSVDNLQASTAFYSQLFGAVPSVLKPDYAKWMLDDPR
                     VNFAISQRGATAGLDHLGIQVESDDELADIKKRLDAAGMVTLSEADTVCCYAKSDKHW
                     VVDPSGIAWEAYHTLDSAPTFMTCRRAGISRPAARRQHKRSSSVRARLDVHDR"
     gene            2103..3146
                     /gene="arsB"
                     /locus_tag="PKW44_04100"
     CDS             2103..3146
                     /gene="arsB"
                     /locus_tag="PKW44_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020183378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HQR50801.1"
                     /translation="MTDKRKIGAFERNLTWWVAGCIAVGILLGKLVPDVFQAIGGFKL
                     AEVNLPVALLIWLMIVPMLLKIDFAAMHEVTQHRNGIAVTLAINWLVKPFSMALLGWL
                     FIRHLFAPMLPAEQIDSYIAGLILLAAAPCTAMVFVWSNLCDGEPKFTLSQVAINDLI
                     MLFAFAPIVALLLGLSSITVPWDTLALSVALFIVAPVILSQFWRKALLARGGKTALQA
                     TLKKLHPLSLSALLATLVLLFAFQGEQILAQPLVIALLAAPILIQVYFNAGLAYWLNR
                     KFKVAHCVAGPSALIGASNFFELAVATAIALFGFESGAALATVVGVLIEVPVMLSVVG
                     IVNRSKSWYEANP"
     gene            3143..3610
                     /locus_tag="PKW44_04105"
     CDS             3143..3610
                     /locus_tag="PKW44_04105"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HQR50802.1"
                     /translation="MNVLFLCTGNSCRSILAEATFNALAPAGMHAMSAGSHPAGYVHP
                     RSLALLRREGIATEGYFSKSWDDLPAIPDIVISVCGNAANETCPAYLGQAVRAHWGVE
                     DPAKATGSDAEINAAFEQAYRILRQRIEEFLALPLVQMDQATLQQELHRIGNL"
     gene            3661..5145
                     /gene="mqo"
                     /locus_tag="PKW44_04110"
     CDS             3661..5145
                     /gene="mqo"
                     /locus_tag="PKW44_04110"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008924 - malate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HQR50803.1"
                     /translation="MSEPVKVDVLLVGSGIMSATLGALLSRLDPTLNILMVERLDQVA
                     LESSDGWNNAGTGHAGYCELNYTPQDTDGNIAIERALTINAAFEVSLQLWTTLVEQGT
                     LPDPAEFINPTPHQSLVWGESDVAFLRRRYQLLSAHHLFRDMEYSEDRDQLNAWMPLV
                     MAQRATSQKLAATRVAYGADVDFGALARSLVKTLQQQAHFELWTGHAIRQLRQAKDGA
                     WHVGVQNRKTGRSRTIRAGFVFLGAGGGTLPLLQKSGIPEGKGYGGFPVSGQWLVCNN
                     PDIIRQHHAKVYGKAALGAPPMSVPHLDTRIINGKPALLFGPFAGFTTKFLKRGSLLD
                     LATSVKPDNLKPTLAAGLRNLGLTRYLVGEALQSHRARMDALRQFVPTASAEEWTLAK
                     AGKRVQIIKQCGEQGGRLEFGTEIVSAQDGSLAALLGASPGASTSVSAMITVIERCFA
                     EKFKNGDWRDRMKALVPSYGESLVDNAELLNSIRSRNLDILRLR"
     gene            5205..5615
                     /locus_tag="PKW44_04115"
     CDS             5205..5615
                     /locus_tag="PKW44_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1924 domain-containing protein"
                     /protein_id="HQR50804.1"
                     /translation="MFHTIMHISLAAVLAIALPAVAQTPAETLAVLKQTAAETPGFHG
                     FSTARGEQFFKSTHGSDWSCATCHTDNPAGVGKHTRTGKAIQPLAPAANADRFTDQAK
                     VDKWFKRNCNDVLNRACTPLEKGDVLTYLMSLAK"
     gene            5626..6075
                     /locus_tag="PKW44_04120"
     CDS             5626..6075
                     /locus_tag="PKW44_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diheme cytochrome c"
                     /protein_id="HQR50805.1"
                     /translation="MRIHLLILTFLVAGAAQASGETLRATNPRWKAECGSCHVAYPAR
                     LLPAESWKAMMAGLDKHFGSDASIDPATTAEILAFLEQNAGRRKPDPAVKPQLRITET
                     HWFKREHDEVPAATWKNPLVKSAANCAACHTRAEQGDFNERSLRLPK"
     gene            6086..6715
                     /locus_tag="PKW44_04125"
     CDS             6086..6715
                     /locus_tag="PKW44_04125"
                     /inference="COORDINATES: protein motif:HMM:NF013460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HQR50806.1"
                     /translation="MLQRILVWDMPTRVFHWLLAISFTGAFLTAETERYRDIHVMLGY
                     TLLGLIAFRLVWGFAGTRYAQFRSFLFAPGQVVDYLKSLVRREPQHYVGHNPAGSLAI
                     FLLLGLGLLAAISGILLYFEVGGEEAFEELHEGAANFMLAVVIIHIAGVVVSSWLHRE
                     NLARAMLTGYKSGEAQQGIPRTHAWLGAIILAAVAAFWVWYPASGTVTG"
     gene            6723..7481
                     /locus_tag="PKW44_04130"
     CDS             6723..7481
                     /locus_tag="PKW44_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR50807.1"
                     /translation="MNRQQQEVFSKVPAITLGFWIIKILATTLGETGGDMVSMSMGFG
                     YLASTGMFAILFLIAVIAQVRAEKFHPTLYWAAIIATTTVGTTLADFMDRSLDIGYAG
                     GAALLLGLLLASLFVWQRALGSVAIDTVNSPKSEMYYWVTIMFSQTLGTALGDWTADT
                     AGLGYGGGVMVFGVMLAALAITYYRTTISHTTLFWAAFILTRPLGAVVGDLLDKPVSE
                     GGLALSRYSATIVIVSLIVACMLVFKQVPASRRH"
     gene            7514..8170
                     /locus_tag="PKW44_04135"
     CDS             7514..8170
                     /locus_tag="PKW44_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HQR50808.1"
                     /translation="MRILVVEDDNLLGDALQAGLKQAGYAVDWLRDGTAADQALSSEP
                     YAATVLDLGLPRMAGLEVLRRLRQRGSALPVLILTARDTVDDRILGLDSGADDYLVKP
                     FDMGELVARLRALVRRASGQPAPTLKLGDIELDPAAHRVSYRGRPAELSAREFAVLQA
                     LMLSAGKVLSRTQLEEQLYAWGEEIESNAVEVHIHHLRRKLTPALIETIRGVGYLIKS
                     "
     gene            8173..9495
                     /locus_tag="PKW44_04140"
     CDS             8173..9495
                     /locus_tag="PKW44_04140"
                     /inference="COORDINATES: protein motif:HMM:NF020110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQR50809.1"
                     /translation="MWHNPSLKKRLLAMVLGVITLVWLGASVWTYFDAREELDEMLDA
                     HLTQTASLLLTQTTHELDELETEHAPQLHKYSRRVAFQVWEQGRDLRLHSVNAPQQPL
                     SDKDSGFSDRTLDNHRWRVFSAWDDSHTYLIHVAEQAEVREKLARGVAANLLRPLLVA
                     LPLLAILLWLAVASGLRPLVGLARQVGRREPDNLAALDAGSAPDEVIPLIERLNTLFH
                     RINASIQSERRFTADAAHELRTPIAVIKAQSQVARNAANTAERSHALDGAIAGCDRAA
                     HLIEQLLTLARLDSVETGSREPCPLRQLAAETLADIAPAALDKGVQLELAEGDEVTVN
                     GLPALLRVMLRNLLDNAVRHTPPGTLVRVEISASNITVSDNGPGLPEQELEKISQRFY
                     RPLGTEASGSGLGLSIVRRIAEMHDAQLTFTSASTGKGLRATVSFRLP"
     gene            9554..>9857
                     /locus_tag="PKW44_04145"
     CDS             9554..>9857
                     /locus_tag="PKW44_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HQR50810.1"
                     /translation="MSDNEFLTNAVNKRRTFAIISHPDAGKTTLTEKLLLFGGAIQMA
                     GTVKARKSGKFATSDWLDIEKQRGISVASSVMQFSYDDCVINLLDTPGHADFSEDTY"
BASE COUNT         1933 a         3210 c         2888 g         1826 t
ORIGIN      
        1 gttgtggcaa gcgctcggtt tgctcgcgcc agccgaggat gaaatcgcgg tatcgctgtt
       61 ctgctacgac actgcaccgc tgcctgcgct gctggacgct tgggctgcgg cagccaggcc
      121 ggtacgctgc ctggtgccgg aaggcaaagc actttcaaag gttgcggcct gtttcgggcg
      181 gtcttcgctt actcccggcg atacgctgca acgcggcaac ctcacgttgc acgtgctgcc
      241 tttcatgccg caggaagact atgacttgct gctgtgggcg tgcgactgca acttcgtgcg
      301 cggcgaggat tccttcgtgc gggcacagtg ggccgccaag cccatggtct ggcatatcta
      361 cccacagcag gaaaacgcgc atcacatcaa actcgacgcc tttctcgacc tttattgcag
      421 cgacctggcg gaggatgctg caacaaccct gcgcgccttt caccacgggt ggaacaacca
      481 agggccgcta tgctgggacg atttccgcaa ccatcacacc gccctgcaag cccacgccgc
      541 agcctgggca ggacgcctgg cggaacaatc cgacctcgca tctaagctgg tgattttttg
      601 caaaaatcgg gtataatgcg cggctttttt acccccatat agcgcaggac atcagatgaa
      661 aacagctcag gaactccgcg taggcaacgt gtttatggtc ggcagcgacc ccatggtcgt
      721 gctcaaggcc gaatacagca agtccggccg caactcttcc gtggtgaaga tgaagttcaa
      781 gaatttgctc accgaatcac ccagcgaagc ggtgtacaag gccgatgaca agtttgacgt
      841 cgtggtgctg gaaaagaagg acgtgaccta ctcctacttt gccgacccga tgtacgtgtt
      901 catggacgcc gaatacaacc agtacgaagt cgaagcggaa accatgagcg acgcgctcaa
      961 cttcctccag gacggcatgc cgtgcgaagt ggtgttctac aacggcaagg ccatctcggt
     1021 ggaactgccc aactccgtgg tgcgcgaaat cacctatacc gaacctgcgg tcaagggcga
     1081 tacttccggc aaagtgatga aacccgccag gaccgccacc ggcttcgaac tgccggtgcc
     1141 gctgttctgc gaaatcggcg acaagatcga aatcgacacc cgcaccctgg aataccgcaa
     1201 ccgcgtgaag taattccaat ttcaggttgg ccagaaagga gccgcaaggc tcttttttgt
     1261 tgcctgtgaa attctcgttg acgagttatc catatttcca gaataataga aatatggata
     1321 acaaaaccgc cgtcggcctg cttacttccc tcgcccagga atcccgcctg agcctcttcc
     1381 gcctgctggt acaggctggc ccgagtggcc tggctgcggg cagcatcgca gagcaactgg
     1441 gcgtacccgc cagcacgttg tcctttcacc tcaaggaact gtcgcacgcc aggctggtac
     1501 aagcgcggca ggacggccgc ttcatctact attccgctga ttttgccgtg atgaatgccc
     1561 tgctcggctt tctcctggaa aactgctgcg gcgggcagca ggattgctgt tccccgatta
     1621 tcgtcaccag caaaggaagc ccatcatgaa acgcatgcac atccatgtct cggtagataa
     1681 cctgcaagca agcacggcct tttacagcca gctgttcggc gcggtgccca gcgtgctgaa
     1741 acctgattac gccaagtgga tgctggacga cccacgggtg aatttcgcca tcagccaacg
     1801 cggcgcgacg gccggactgg accatctcgg catccaggtg gaaagcgacg acgaactggc
     1861 cgacatcaag aagcggctgg atgcagccgg catggtgacg ctgagcgaag ccgacactgt
     1921 ctgctgttac gccaagtccg acaagcactg ggtggtggat ccgtctggca ttgcgtggga
     1981 ggcttaccac acgctggata gcgcacccac cttcatgacc tgccggagag cggggatcag
     2041 ccggcctgct gcgcgccgcc agcacaaaag atcgagttcc gttcgcgcaa ggctggatgt
     2101 gcatgaccga taaaagaaaa atcggcgcct tcgagcgcaa cctgacctgg tgggtggcgg
     2161 gctgcatcgc cgtcggcatc cttctgggca agctggtacc ggacgtgttc caggccatcg
     2221 gcggcttcaa actggccgag gtcaacctgc cggtggcgct gctaatctgg ctgatgatcg
     2281 tgcccatgct gctcaagatc gactttgccg ccatgcacga agtcacccag caccgcaacg
     2341 gcatcgccgt cacgctcgcc atcaactggc tggtcaagcc gttctccatg gcgctgctgg
     2401 gatggctgtt catccgccac ctgttcgcgc ccatgctgcc ggccgaacag atcgacagct
     2461 acatcgccgg gctgatcctg ctcgccgccg cgccttgcac cgccatggtg ttcgtgtgga
     2521 gcaacctgtg cgacggcgaa cccaagttca ccctgtcgca ggtggcgatc aacgacctca
     2581 tcatgctgtt cgccttcgcc cccatcgtcg ccctgttgct gggcttgtcc agcatcacgg
     2641 tgccatggga tacgctggcg ctttccgtgg ctctcttcat cgtagcgccg gtcatcctca
     2701 gccagttctg gcgcaaagcc ttgctggcta ggggcggcaa aacggccttg caagccaccc
     2761 tgaaaaaact gcacccgctt tcactgtccg ccctgctcgc cacgctggtg ctgctgttcg
     2821 ccttccaggg cgagcagatc ctggcgcagc cgctggtaat cgcgctgctg gccgcgccca
     2881 tcctgatcca ggtctatttc aacgccgggc tggcctactg gctcaacaga aaattcaaag
     2941 tcgcgcattg cgtggccggg ccatcggcgc tgatcggcgc ttccaacttc ttcgaactgg
     3001 cggtggccac cgccatcgcc ctgttcggct tcgaatccgg cgccgcactg gccaccgtgg
     3061 tcggcgtgct gatcgaagtg cccgtcatgc tatccgtggt cggtatcgtc aaccgttcca
     3121 aatcctggta tgaggccaat ccatgaatgt tctctttctt tgcaccggca actcctgccg
     3181 ctccatcctg gcggaagcca ccttcaacgc ccttgccccg gccggtatgc acgccatgag
     3241 cgccggcagc catccggcag gttacgtgca cccgcgatcg ctggcactgc tgcgacgcga
     3301 gggtattgcc accgaaggct acttcagcaa atcctgggat gacctgccgg ccatacccga
     3361 catcgtcatc tccgtatgcg gcaatgccgc caatgaaacc tgcccggctt atctgggcca
     3421 agcggtacgc gcccactggg gcgtggaaga cccggccaag gccactggca gcgacgccga
     3481 aatcaacgcc gcattcgaac aggcctaccg catcctgcgc cagcgcatcg aggaatttct
     3541 ggcgctaccg ctggtgcaaa tggatcaagc cacgctgcaa caggagctgc accgtatcgg
     3601 caacctgtag gaacaccctg catgtaaaat agccgggttt tcatcccgat ccttttaccc
     3661 atgtccgaac ccgtcaaagt cgatgtcctg ctagtcggca gcggcatcat gagcgccacg
     3721 ctcggcgccc tgctgtcccg gctagacccc actctgaaca tcctcatggt ggagcgcctg
     3781 gaccaggtcg ccctggaaag cagcgacggc tggaacaacg ccggcaccgg ccacgccggc
     3841 tattgcgaac tcaactacac cccacaggac acggacggca acatcgccat cgagcgagcg
     3901 ctgacgatca acgccgcgtt cgaggtttcg ctgcaattgt ggaccacgct ggtggaacag
     3961 ggcaccctgc ccgaccctgc cgaattcatc aaccccacgc cacatcagag cctggtctgg
     4021 ggcgaaagcg acgtcgcctt cctgcgccga cggtatcagc tgctgagtgc gcatcatttg
     4081 ttccgggaca tggagtacag cgaggaccgc gaccagttga atgcgtggat gccgctggtg
     4141 atggcgcagc gcgcaaccag ccagaagctc gccgcgacgc gcgtggccta tggcgcggac
     4201 gtggatttcg gcgcgctggc gcgcagcctg gtcaagacat tgcaacaaca agcccatttc
     4261 gagctgtgga ccgggcacgc catccgccag ctcaggcagg cgaaggatgg cgcctggcat
     4321 gtcggcgtgc agaaccgcaa gaccgggcgc agcagaacca tccgcgccgg tttcgtcttc
     4381 ctcggcgccg gcggcggcac cctgccgctg ctgcaaaaat ccggcatccc cgaaggcaag
     4441 ggctacggcg gctttccggt cagcgggcaa tggctggtgt gcaacaaccc ggacatcatc
     4501 cgccagcacc acgccaaggt ctacggcaag gccgcgctgg gcgcgccgcc gatgtcggtg
     4561 ccgcatctgg acacgcgcat catcaacggc aagccggcgc tgctgttcgg ccctttcgcc
     4621 ggcttcacca ccaaattcct caagcgcggc tcgctgctcg atctcgccac ctcggtcaag
     4681 ccggacaacc tcaagcccac cttggccgcc ggtctgcgca acctgggcct gacccgctac
     4741 ctggtcggcg aggcgctgca atcgcaccgg gcacgtatgg acgccctgcg ccagttcgtc
     4801 cccaccgcca gcgcggaaga atggactctg gccaaggccg gcaagcgcgt gcagatcatc
     4861 aagcaatgcg gcgaacaagg cggccggctg gaattcggca ccgaaattgt ttcagcccag
     4921 gacggctcgc tggctgccct gctcggcgcc tcccccggcg cttcgacctc ggtatccgcc
     4981 atgatcacgg tgatcgaacg ctgcttcgcc gaaaaattca agaacggcga ctggcgcgac
     5041 aggatgaaag cactggtgcc gtcttatggc gagtcgctgg tggataacgc cgaactgctg
     5101 aacagcatca gatcgcgcaa cctcgatatt ctgcgcttgc gttaagtctg ccttaaggtt
     5161 gacctcctaa tctgcacaca tcacccgtgc aaaaggaggt cgccatgttc cacaccatca
     5221 tgcacatcag cctggccgcc gtactcgcca ttgcactgcc ggctgtcgcg caaactccgg
     5281 ccgaaaccct ggccgtactg aagcagaccg ccgctgaaac gccaggcttc catggctttt
     5341 ccactgcgcg cggggagcaa ttcttcaaat ccacccacgg cagcgactgg agctgcgcca
     5401 cctgccatac cgacaacccg gccggcgtgg gcaagcatac ccgcaccggc aaggccatcc
     5461 agccgctggc accggcggcc aatgccgacc gtttcaccga ccaggccaag gtcgacaagt
     5521 ggttcaagcg caactgtaac gatgtgctga accgtgcctg tacgccgctg gaaaagggcg
     5581 acgtactgac ctatttgatg tccctggcta aataaggaga caaccatgcg aattcacctg
     5641 ctcatactga catttctggt tgccggcgcc gcgcaagcct ccggcgaaac gctccgtgcc
     5701 actaacccaa gatggaaggc cgagtgcggc tcctgccatg tcgcctaccc cgcgcgcctg
     5761 ctgccggcgg agtcgtggaa agcgatgatg gccgggctgg ataaacattt cggctccgat
     5821 gccagcatcg acccagccac cacggcggaa attctcgctt tcctcgaaca aaacgccggc
     5881 cgccgcaaac cggatccggc ggtaaaacct cagctgcgca tcaccgaaac ccattggttc
     5941 aagcgcgagc acgacgaagt gccggctgcg acctggaaaa acccgctggt gaaaagcgcc
     6001 gccaactgcg cggcctgcca cacgcgggcc gagcagggcg atttcaacga acgcagcctg
     6061 aggctgccaa aataaggaga acatcatgct gcaacgcatc ctggtttggg atatgcccac
     6121 tagagtcttc cactggctgc tggcgatttc gttcaccggc gccttcctca ccgccgaaac
     6181 cgagcgctac cgcgacatcc acgtcatgct tggctacacg ctgctggggc tgatcgcctt
     6241 ccgcctggtg tggggatttg ccggcacgcg ctacgcgcaa ttccgctcct tcctgttcgc
     6301 gcccggccag gtggtggatt acctgaaaag cctggtgcgg cgcgagccgc aacactatgt
     6361 cggccacaac ccggcgggca gcctggccat cttcctgcta ctcgggctgg gactgctggc
     6421 tgcaatcagc ggcatcctgc tgtatttcga agtcggcggc gaggaagcgt tcgaggaact
     6481 gcacgagggc gcggccaact tcatgctggc ggtggtcatc atccacatcg ccggggtggt
     6541 ggtcagcagt tggctgcacc gcgaaaacct ggcgcgtgcc atgctcaccg gctacaagtc
     6601 cggcgaggcg cagcaaggca tccctcgcac gcatgcctgg ctgggtgcca taatactggc
     6661 tgcggtggcc gcgttctggg tttggtatcc tgccagcggg acggttactg gttgaggatt
     6721 gaatgaacag gcagcagcag gaggttttca gcaaggttcc cgccatcacc ctggggttct
     6781 ggatcatcaa gatcctggcc accaccctgg gtgaaaccgg cggggacatg gtttccatgt
     6841 cgatgggctt cggctacctt gccagcacgg gcatgtttgc catcctgttt ttgattgctg
     6901 tcatcgccca ggtgcgcgct gaaaaattcc atcccaccct ttactgggcg gccatcatcg
     6961 cgaccaccac cgtgggaacg accttggccg acttcatgga tcgttccctc gacatcggct
     7021 atgcgggcgg agcggcgctc ttgctgggct tgctgcttgc ttcgctattc gtttggcagc
     7081 gcgctctggg ctcagtcgcc attgacacgg tgaattcgcc caaatcggag atgtactact
     7141 gggtgaccat catgttttcc caaaccctgg gtaccgcgct gggtgattgg acggcggaca
     7201 ccgctggcct aggttatggt ggcggggtaa tggtatttgg cgtcatgctg gccgcactgg
     7261 cgattaccta ttaccggacg accatctccc ataccacgct attctgggct gcgttcatcc
     7321 tgacgcgccc gctcggcgcg gtagtcgggg atttgctgga caaaccagtc agcgaggggg
     7381 gcttggcact cagccgctac tccgcgacaa tcgtcattgt tagtttgatc gtggcttgca
     7441 tgctggtttt caaacaggtt ccggcaagcc gacggcattg aggccagcac ggaaatgaac
     7501 agtgggagtg aatgtgcgca tacttgtcgt cgaggatgat aacctgctgg gcgacgccct
     7561 gcaggccggc ttgaaacagg ccggctacgc ggtggactgg ctacgcgacg gcacggccgc
     7621 cgaccaggcg ctgtccagtg aaccctacgc ggcgacggta ctggatcttg gactaccgcg
     7681 catggccggg ctggaagtgc tgcgccgcct gcggcagcgc ggctcagctc tgccggtact
     7741 gatcctcact gcccgcgaca cggtggacga ccgcattctg ggtctggaca gcggtgccga
     7801 cgattacctg gtcaaaccct tcgacatggg agaactggtg gcgcggctgc gtgccttggt
     7861 gcgccgcgcc agcggccagc ccgcccccac gctcaagctc ggcgatatag aactcgatcc
     7921 ggcggcgcat cgcgtcagct atcggggtcg gccagcagag ctgtccgcgc gtgaattcgc
     7981 cgtgctgcag gcattgatgc tgagtgccgg caaggtgctg tcacgcaccc aactggaaga
     8041 gcagctttac gcctgggggg aggaaatcga aagcaacgcc gtcgaggtgc acatccacca
     8101 cctgcgccgc aagctcaccc cggcgctgat cgaaaccata cgcggcgtcg gctacctcat
     8161 caaatcctga ccatgtggca taacccctct ctgaaaaaac gactgctggc catggtgctt
     8221 ggggtcatta cgctagtctg gctgggcgcg tcggtgtgga cctatttcga cgcacgcgag
     8281 gaactggacg agatgctgga tgcccacctg acgcaaaccg cctcgctgct gctcacccag
     8341 accacgcacg aactcgacga gctcgaaacc gagcatgcgc cgcagttgca caaatactcg
     8401 cgccgggtgg ccttccaggt ctgggaacag ggccgtgatt tgcgcctgca ttccgtcaac
     8461 gcgccgcagc agccgctgtc ggacaaggac tccggcttca gcgaccgtac cctggataac
     8521 catcgctggc gcgtattcag tgcctgggat gattcgcaca cctatttgat tcacgtcgcc
     8581 gagcaggcgg aggtgcgaga gaaactggca cgtggcgtcg cggccaatct gctgcgaccg
     8641 ctgctggtcg ccctgccgtt gctggccatc ctgctatggc tggctgtcgc cagcggcttg
     8701 cgcccgctgg tcgggctggc gcgacaagtg ggacgccgcg aaccggacaa cctggccgcg
     8761 ctggatgccg gttccgcgcc ggacgaggtc ataccgttga tcgaacggct gaacacgctg
     8821 ttccaccgca tcaacgcctc catccagagc gagcggcgtt tcactgccga cgccgcgcac
     8881 gaactgcgca cccctatcgc cgttatcaag gcgcagtccc aggtggcgcg caacgcagcc
     8941 aacacggccg agcgcagtca tgcgctggat ggcgccattg ccggctgcga ccgcgccgcc
     9001 cacctgatcg agcaactgct gacgctggcc cggctggaca gcgtcgaaac tggctcacgc
     9061 gaaccctgcc cgttgcgcca actggccgct gaaaccctcg ccgacatcgc cccggccgcg
     9121 ctggacaagg gggtgcaact ggagctggcg gaaggcgatg aagtcaccgt caacggtctg
     9181 ccggcgcttt tgcgcgtgat gctgcgcaac ctgctcgaca acgccgtacg ccatacccca
     9241 cctggcacgc tggtacgtgt ggaaatttcc gctagcaaca ttaccgtcag cgacaatggc
     9301 cccggcctgc cggagcaaga actggaaaaa atctcacagc gtttctaccg gccgctgggc
     9361 accgaagcca gcggcagcgg cctcgggctt tccattgtca gacgcatcgc cgagatgcat
     9421 gatgcacaac taacgtttac ctccgccagc actggtaagg gattgcgggc aaccgtcagt
     9481 ttcaggttgc cctgagacgc aagtcacggg agaatagcgc ctttttcgtc gcgcctcacc
     9541 ctgaagaaac ccgatgtctg acaacgaatt tctcaccaac gccgtcaaca agcgccgcac
     9601 cttcgccatc atctcccacc ccgatgccgg caagaccacg ctgaccgaaa aactgctgct
     9661 gttcggcggc gccatccaga tggccggcac ggtgaaggcg cgcaaaagcg gcaagttcgc
     9721 cacctcggac tggctggaca tcgagaagca gcgcggcatt tccgtcgcca gctcggtgat
     9781 gcagttcagc tacgacgatt gcgtcatcaa tctgctcgac acgcccggcc acgccgattt
     9841 ctcggaagac acctacc
//
LOCUS       DAOMFA010000055         9752 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_755_length_9752_cov_5.252965, whole genome shotgun sequence.
ACCESSION   DAOMFA010000055 DAOMFA010000000
VERSION     DAOMFA010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 9752)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9752)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9752
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_755_length_9752_cov_5.252965"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            80..364
                     /locus_tag="PKW44_04150"
     CDS             80..364
                     /locus_tag="PKW44_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HQR50811.1"
                     /translation="MTKSELIAKLSERFPQLVVKDAELSVKAILDAMTDKLSAGERIE
                     IRGFGSFSLNYRPPRLGRNPKTGAKVQVPEKYVPHFKAGKELRERVDGES"
     gene            439..699
                     /locus_tag="PKW44_04155"
     CDS             439..699
                     /locus_tag="PKW44_04155"
                     /inference="COORDINATES: protein motif:HMM:NF018058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HQR50812.1"
                     /translation="MRYFYSAFGFFVFLLALSFGLKNAQPITLHYYLGIAWDAPLILV
                     LVFTFFCGVIAGLLACLSLYVRHRKQLMAMQKELRALESNQE"
     gene            705..1877
                     /gene="lapB"
                     /locus_tag="PKW44_04160"
     CDS             705..1877
                     /gene="lapB"
                     /locus_tag="PKW44_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HQR50813.1"
                     /translation="MLEFEYWWLLALPLFFALGWLTARFDIDQLLSESSALPAAYFRG
                     LNFLIREQHDKAIEAFIEAVQANTESLELHFALGSLFRRRGEIERAIHLHQGLLERPQ
                     LSDLQKTAITAELANDFLKAGLFDRAEQLFKALCAAPGYQHSALRSLLDIYIREREWS
                     RAIETADELERLSGVSFRKEVAQFHCELAAKAIHAVDIGTASQELETALHINKHCIRA
                     TVMQGEIEAAAGNTANTLAYWQRIEKQRPQYLGLVAARVLDGYRSLAQGEQGLQLLHG
                     WVEKYKLPSVLNVVYEATLQEQGAEAAAQLARVELAKKPSLNILERLLQARAMEKQGD
                     DQDVKLMQNTVHHFLGNSRSYCCEQCGFKARRYYWQCPGCNGWETFPPEPREQPLR"
     gene            1874..2572
                     /gene="pyrF"
                     /locus_tag="PKW44_04165"
     CDS             1874..2572
                     /gene="pyrF"
                     /locus_tag="PKW44_04165"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018292898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HQR50814.1"
                     /translation="MNDPKIIVALDYAEARQALQLVGQLDPALCRLKVGKELFTVAGP
                     RLVEQLIEKNFGVFLDLKFHDIPNTAAKACQAAAQLGVWMLNVHASGGRAMMQAAREA
                     VDKTPGTPLLIAVTVLTSMDEPALHEIGVASTLQDQVSRLASLTRDCGLDGVVCSAQE
                     APTLRQSLGQDFCLVTPGIRPAGSGKDDQSRIVTPSTALRQGSSYLVIGRPITQVPDP
                     LQALHAIHQEISVI"
     gene            2679..2927
                     /locus_tag="PKW44_04170"
                     /pseudo
     CDS             2679..2927
                     /locus_tag="PKW44_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479287.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
     gene            complement(3118..5181)
                     /gene="metG"
                     /locus_tag="PKW44_04175"
     CDS             complement(3118..5181)
                     /gene="metG"
                     /locus_tag="PKW44_04175"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HQR50815.1"
                     /translation="MSRKILVTSALPYANGSIHLGHLVEYIQTDIWVRFQKMQGNACH
                     YVCADDTHGTPIMLRAEKEGITPEALIERVHGEHSADFRDFLVEFDNYYSTNSEENRE
                     LASSIYQALKANGKIATRTIEQYYDPVKQMFLPDRFIKGECPKCHAKDQYGDSCEVCG
                     ATYSPTELINPYSAVSGAAPERRETEHYFFKLSECEEFLKGWTRSGTLQEEAANKMGE
                     WFSSGLSDWDISRDAPYFGFEIPGAPGKYFYVWLDAPIGYMASFKNLCAKTGLNFDAY
                     WQKDSQAELYHFIGKDILYFHALFWPATLEYSGYRKPTKVFAHGFLTVNGEKMSKSRG
                     TFITARSYLEHVRNPEYLRYYYAAKLNGSMEDIDLNLEDFVARVNSDLVGKYVNIDSR
                     CAGFVSKRFGGELGAIDAGATVEFESAFQSDEIARSYDDRDYGRALREIMRLADFANQ
                     YIADRKPWEMAKQVGRETELHEVCSTALTLFRDLTLYLKPVIPGLAEKVEKFLNIPPL
                     MWGNVWSPLPVGHVINEYQHLITRLDPKQIEAMVEANKESLQVPQQHSEQRHAAHQQH
                     VAETAVTGKEEGAYVSIDDFAKVDLRIARIASAEHVEGAEKLLRLTLDIGEEQPRQVF
                     AGIKSAYDPAALVGRLTVMVANLAPRKMKFGMSEGMVLAASDERGGPYILSADSGAEP
                     GMRVK"
     gene            5297..6385
                     /gene="apbC"
                     /locus_tag="PKW44_04180"
     CDS             5297..6385
                     /gene="apbC"
                     /locus_tag="PKW44_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HQR50816.1"
                     /translation="MPLSELQIQTALKEIADPNTGKDFISSKSARNIRIDGNNVALDI
                     VLGYPANSVLAGVQALVQDKLKTLPEMGSVFVNVTSKIVPHSVQRGVQLIPGVKNIIA
                     VASGKGGVGKSTTAVNLALALAAEGARVGILDADIYGPSQPQMLGISARPESTDGKSM
                     EPLERYGIQAMSIGFLIDMDTPMVWRGPMVTGALEQLLRETNWRDLDYLVIDLPPGTG
                     DIQLTLSQKVPVTGAVIVTTPQDIALLDARKGLKMFEKVGVPIIGIVENMSTHICSQC
                     GHEEHIFGTGGGAQMCQDYDVELLGSLPLDIRIREQADSGMPTVATEPDGKIAAVYKQ
                     IARRAAIKLSQMALDHSSKFPNIVIQNT"
     gene            6552..7640
                     /gene="aroG"
                     /locus_tag="PKW44_04185"
     CDS             6552..7640
                     /gene="aroG"
                     /locus_tag="PKW44_04185"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019897007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HQR50817.1"
                     /translation="MNTPHKPATDDVRIREIKELTPPSRILAELTASAAASDNIVATR
                     QEIHRILHGADDRLLVIIGPCSIHDHDAAIEYAKLLLEQRKRLSADLLIVMRVYFEKP
                     RTTVGWKGLINDPHLDGSFRINEGLRLARRILIEVNELGMPAGNEFLDVITPQYTADV
                     VSWGAIGARTTESQVHRELASGLSCPVGFKNGTDGNTRIAVDAIKAAASAHHFLSVTK
                     AGHSAIVSTAGNEDCHLILRGGKQPNYDTASVDAACKELGIAGVAPRVMIDCSHANSR
                     KDFRLQLDVARDVAGQVAAGDQRIFGLMVESHLKEGRQDLEPGCALEYGKSITDACLG
                     WEDSVRLLEILAEGVRARRRLYEAEEED"
     gene            7721..8293
                     /gene="dcd"
                     /locus_tag="PKW44_04190"
     CDS             7721..8293
                     /gene="dcd"
                     /locus_tag="PKW44_04190"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HQR50818.1"
                     /translation="MSIKSDKWIRRMAEQHGMIAPFEPGQVKEANGHKLVSYGTSSYG
                     YDIRCSSEFKLFTNINSTIVDPKNFDPNSFVEVDADYCIIPPNSFALARTVEYFRIPR
                     NVLTVCLGKSTYARCGIIVNVTPFEPEWEGYVTLEFSNTTPLPAKIYANEGCAQVLFF
                     EADADDVCETSYKDRGGKYQGQHGVTLPKI"
     gene            8298..8609
                     /locus_tag="PKW44_04195"
     CDS             8298..8609
                     /locus_tag="PKW44_04195"
                     /inference="COORDINATES: protein motif:HMM:NF018151.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeA/HdeB family chaperone"
                     /protein_id="HQR50819.1"
                     /translation="MTTRLLCCFAMVMLNITATFADDKPKNIDLSNFTCKEFLHSMQN
                     GHEQEMRSLAMWLDGYLGGISGNTEVDWQELKQYTDELITYCRNNPASVMREAARKAG
                     M"
     gene            8636..>9752
                     /locus_tag="PKW44_04200"
     CDS             8636..>9752
                     /locus_tag="PKW44_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Orn/Lys/Arg decarboxylase N-terminal
                     domain-containing protein"
                     /protein_id="HQR50820.1"
                     /translation="MKFRFPVIIIDEDFRSENASGLGIRVLAKAIEDEGMEVLGVTSY
                     GDLTSFAQQQSRASAFILSIDDEEFVEESKTALDSLRKFVEEIRYRNEEIPIFLHGET
                     RTSRHIPNDVLRELHGFIHMNEDTPEFVARYIAREARTYLDSLPPPFFRALTHYAADG
                     SYSWHCPGHSGGVAFLKSPVGQMFHQFFGENMLRADVCNAVEELGQLLDHTGPVAASE
                     RNAARIFNADHLYFVTNGTSTSNKIVWHSTVAPGDIVVVDRNCHKSVLHSIMMTGAVP
                     IFLMPTRNNFGIIGPIPKAEFAWESIRRKIAANPFATDKNAKPRVLTITQSTYDGIIY
                     NVEEIKKELDGRIDTLHFDEAWLPHAAFHDFYGDYHAI"
BASE COUNT         2139 a         3036 c         2710 g         1867 t
ORIGIN      
        1 caatgccggc actaccagtc tgggggcgct gctcaaggcc aagcttgacg gtaaaaacaa
       61 cgaataaggc gttgatgcca tgaccaaatc cgagctcatc gccaaactgt cggagcgctt
      121 cccgcagctg gtcgtcaagg atgcagaatt gtcggtcaag gcaattctgg acgccatgac
      181 cgacaagctt tctgccggtg agcgcatcga gatccgcggg ttcggtagtt tcagtctgaa
      241 ctaccgcccc ccccgtctcg ggcgcaaccc caagactggt gcaaaggtgc aggtaccgga
      301 gaaatatgtc ccgcacttca aggccggcaa ggaactacgc gaacgcgtgg atggcgagtc
      361 ctgaaagttg tattgtccac aaaacggcag aacgcaagtt ctgccgtttt tttattccga
      421 tttaggtaaa atgtgctcat gcgctacttt tactccgcat tcggcttttt cgtattcctg
      481 ctggcactca gcttcggtct caagaatgcc cagcctatca ccctgcacta ttatctcggc
      541 atcgcctggg atgccccgct gatcctggta ttggttttca cttttttttg cggcgtcatt
      601 gcaggattgc tggcctgcct ttcgctctat gtcaggcatc gcaaacaact gatggcgatg
      661 caaaaagaac tgagagccct ggaatccaac caggaataaa caacatgctg gaattcgaat
      721 actggtggct gttggcgcta cccctgtttt tcgcacttgg atggctaacc gcccgtttcg
      781 acattgacca gctgctatcc gaatccagcg ccttacctgc cgcctacttc agggggctga
      841 atttcctgat ccgcgagcag cacgacaagg ctatcgaggc ttttatcgag gccgtgcagg
      901 ccaacaccga atcgctggag ctgcatttcg ctctgggcag cctgttccgc cgccgcggtg
      961 aaatcgagcg cgctatccac ctgcaccagg gattgctgga acgcccccag ctttccgacc
     1021 tgcaaaaaac cgccatcacg gccgaactgg ccaacgactt cctcaaggcc gggctgttcg
     1081 atcgcgccga gcaattgttc aaggcgctat gcgcggcgcc tggctaccag cactcggcgt
     1141 tgcgctcgct gctggacatc tacatccgcg aacgcgagtg gtcacgcgcc attgagactg
     1201 ccgacgagct ggaacgtctt tccggcgtct cgttccgcaa ggaagtcgcc cagtttcatt
     1261 gcgaactggc ggccaaggcc atccatgcag tggacatcgg gacagccagc caggaactgg
     1321 aaaccgcgct gcatatcaac aagcattgca tccgcgccac tgtcatgcag ggcgaaatcg
     1381 aagcggccgc cggcaacacg gccaacaccc tcgcctattg gcagcgcatc gaaaagcaac
     1441 ggccgcagta tctcggcttg gttgctgcgc gcgtactgga tggctatcgt tccctcgccc
     1501 agggcgaaca aggtctgcaa ctgctgcatg gctgggtgga aaaatacaaa ctgccttccg
     1561 tgctgaatgt ggtgtacgaa gcgactctgc aggagcaagg cgccgaagcg gcagcacaat
     1621 tggcgcgagt ggagttggca aagaaaccca gcctcaacat tctcgaacga cttctgcagg
     1681 cacgggccat ggagaagcaa ggcgacgacc aggatgtgaa gctgatgcag aatacggtgc
     1741 atcatttcct cggcaacagc cgcagctact gctgcgagca gtgcggcttc aaggcgcgtc
     1801 gctactactg gcaatgcccc ggctgcaacg gctgggaaac attcccgccg gaaccgaggg
     1861 agcagcccct gcgatgaacg acccgaaaat cattgtcgca ctcgactacg ccgaagccag
     1921 gcaggcgttg caactggtcg gccaactcga ccccgcacta tgccgcctca aggttggcaa
     1981 ggaattgttc actgtcgccg ggccacgcct ggtcgagcaa ttgatcgaga aaaacttcgg
     2041 cgtttttctc gacctcaaat ttcacgatat ccccaacact gcagccaaag cctgccaggc
     2101 agcggcgcaa cttggcgttt ggatgctgaa cgtccatgca tccggcggac gcgccatgat
     2161 gcaagcggca cgcgaagcgg tggataaaac ccccggcacg ccgctgctga tcgcagtgac
     2221 cgtactgacc agcatggatg aacccgcatt gcacgagatc ggcgtcgcca gcacgttgca
     2281 ggatcaggta tcacgtctcg ccagcctgac cagggactgt gggctggatg gcgtggtctg
     2341 ttcggcgcag gaagcaccca cgctccgcca gtccctcggc caggacttct gcctggtcac
     2401 ccctgggata cgccccgccg gcagcggcaa ggacgaccag tcccgcatcg tgacgccatc
     2461 caccgcgcta cgccagggct ccagttacct ggtcatcggc cgtcccatca cgcaagtgcc
     2521 tgacccattg caggcattgc atgccattca tcaggaaatc tcggtgattt agtttcgcta
     2581 ttggcaacca gccggcttga tgttagcttg ctggttgcac gatgtgttgg tagacactgc
     2641 agggaatttc tttttactcg aactgaaaag ggaaaaccat gaaaaaactg ttgatcgcaa
     2701 ttgccgtatc tctgatgttc actctgcctg catgggcagc ggtagacctc aacaccgcca
     2761 gccaggccga actggaaacc gtgaagggca tcgggccgac caaagccaag gccattattg
     2821 attaccgtaa aaagaacggt aatttcaagt ccgtggatga tctggacaac gtgcccggat
     2881 tcggcaaggc caacgtcgaa aaggtaaaaa aagaaatcac tgtaggcaat gcaaaggctg
     2941 ccagcgccgg caagccggaa aaaaccgtca agcctgcagc cccggcggca aaaccagtca
     3001 cccccgcgac tccagcacct gccgcaacgg acaagagcgc aaagaagaag taagcattct
     3061 gccgccttgg cgtacaaaaa agccccgcaa tgcggggctt ttacttgacc agtgcgctta
     3121 cttcacccgc attccaggtt ccgcaccaga gtctgctgac aggatgtacg gcccgccgcg
     3181 ctcatccgag gcggccagca ccatgccttc cgacatcccg aacttcattt tgcgcggcgc
     3241 gagattggcc accatgacgg tcagtcgccc aaccagtgcc gcaggatcat aggccgactt
     3301 gatgccggcg aacacttgcc gcggctgttc ctcgccgata tccaaggtca acctgagcag
     3361 cttctctgcc ccctcgacgt gctcggcact ggcaatgcgc gcaatgcgca ggtcgacctt
     3421 agcgaaatcg tcgatggaaa cataggcgcc ctcctccttg ccggtcacgg cagtttcggc
     3481 cacatgctgc tggtgcgcag catggcgctg ctcggaatgt tgttgcggca cttgcaaaga
     3541 ctccttgttg gcttcgacca tggcttcgat ctgctttggg tcgagacggg taatcaggtg
     3601 ttgatattcg ttaatcacgt gaccaactgg caatggtgac caaacattgc cccacatcaa
     3661 tggcggaata ttgaggaatt tttcaacttt ctcagccagc cccggtatga ctggtttgag
     3721 atacagggtc aggtcgcgga acaaggtcag tgcagtagaa caaacctcat gcaactccgt
     3781 ttcccgacca acctgcttgg ccatttccca aggtttccga tcagctatgt attgattggc
     3841 aaaatcagcc agcctcataa tttcgcgcaa agctcggcca taatcgcgat catcatagct
     3901 tcgggcaatt tcgtcactct ggaaagccga ttcaaattcg actgttgcac cagcatcaat
     3961 cgcccccaac tcccccccaa agcgcttgct gacaaacccc gcacagcggc tgtcaatatt
     4021 gacgtacttg cccaccaggt cggaattcac ccgcgccacg aagtcctcca ggttgaggtc
     4081 gatgtcttcc atgctgccgt tgagcttggc ggcgtagtag taacgcaggt attccgggtt
     4141 cctgacgtgc tccaggtagc tgcgtgccgt gatgaaggtg ccgcgcgact tggacatctt
     4201 ttcgccgttg acggtgagaa agccatgggc aaatactttg gtcggcttgc ggtagccgga
     4261 gtattccagc gtagcgggcc agaacagggc gtggaagtag agaatatcct tgccgatgaa
     4321 gtggtacagc tcggcctgac tatccttttg ccagtaggcg tcgaaattca gtccggtctt
     4381 ggcgcacagg ttcttgaagc tggccatgta gccgatcggc gcatccagcc agacatagaa
     4441 atacttgcca ggagcaccag gaatctcgaa accaaaataa ggcgcatcgc gcgagatgtc
     4501 ccagtccgac aggccggagg agaaccattc gcccatcttg ttggcggcct cttcctgcag
     4561 ggtgccggag cgcgtccagc ctttcaggaa ttcctcgcac tcggacaact tgaagaaata
     4621 atgctcggtt tcgcggcgct ccggcgcggc gccggaaacg gcggaatacg ggttgatcag
     4681 ctcggtcggg ctgtaggtgg cgccgcacac ttcgcaggaa tcgccgtact ggtccttggc
     4741 gtggcacttg gggcactcgc ccttgatgaa gcgatcgggc aggaacatct gcttgaccgg
     4801 gtcgtagtac tgctcgatgg tgcgcgtggc gatcttacca ttggccttga gtgcctggta
     4861 gatactgctc gccaactcgc gattttcctc ggagttggtc gaatagtaat tgtcgaactc
     4921 gaccagaaaa tcgcggaaat ccgcgctgtg ctcgccatgc acccgctcga tcagcgcttc
     4981 cggggtgatg ccctctttct cggcgcgcag catgatgggc gtgccatggg tgtcgtcagc
     5041 gcagacatag tggcacgcgt tgccctgcat cttctggaaa cgtacccaga tgtcagtctg
     5101 gatgtattcc accagatgtc caagatggat gctgccattg gcataaggca gcgcggaggt
     5161 gacgagaatt ttgcgggaca ttttcggcgg cggctatgta agaatttcgt attttaacag
     5221 cctgcatgca aatgacgcag cccagcttat cagctagaat tattacttga tcgtattgct
     5281 caggaatcac cccttcatgc cactctccga actgcaaatc cagaccgcgc tcaaggaaat
     5341 cgccgatccc aataccggca aggatttcat ctccagcaaa tcagcccgca acatccgcat
     5401 cgacggcaac aacgtcgcgc tcgacatcgt gctcggctac ccggccaatt ccgttcttgc
     5461 cggggtacag gcactggtgc aggacaagtt gaagacattg cccgaaatgg gcagtgtgtt
     5521 cgtcaacgtg accagcaaaa tcgtccccca ttcggtgcag cgcggtgtgc agttgattcc
     5581 gggtgtcaag aacatcatcg ccgtcgcctc cggcaagggc ggcgtcggaa aatccaccac
     5641 cgcggtcaac ctggcgctgg cgctggccgc agaaggtgca cgggtcggca tcctcgacgc
     5701 cgacatctac ggcccctcgc agccgcagat gctgggcatc agcgcgcgcc cggaaagcac
     5761 cgacggcaag agcatggagc cgctggaacg ctacggcatc caggccatgt ccatcggctt
     5821 cctgatcgac atggacaccc ccatggtgtg gcgcggtccc atggtcaccg gcgcgctgga
     5881 acaactgctg cgcgaaacca actggcgcga cctggattac ctggtcatcg acctgccacc
     5941 cggcaccggt gacatccagc tcacgctgtc gcagaaagtc ccggtcaccg gcgccgtcat
     6001 cgtcaccacg ccacaggaca ttgccttgct ggacgcacgc aagggactca agatgtttga
     6061 gaaggtcggc gtgcccatca tcggtatcgt ggagaacatg agtacgcata tttgcagcca
     6121 gtgcggccac gaggagcata tcttcggcac cggcggcggc gcgcaaatgt gccaggatta
     6181 cgacgtggag ctgttggggt cgctgccgct ggacatcaga atccgcgaac aggccgacag
     6241 cggaatgccc accgtagcca ccgagccgga tggcaagatc gccgccgtat acaagcagat
     6301 cgcccgccga gccgccatca agctgtcaca gatggcgctg gatcacagca gcaagttccc
     6361 gaacatcgtg attcagaata cgtaataccc gccccggcat ttaccggagg gcaggtattt
     6421 gcttcagcgg cggtaaaaaa ccctttccca tcaagccgca acctgtaatt ttaggtgtcc
     6481 acgcatgcct cgatgcgtta aaatgcgcct cttttatttt ccgcggcccc tggccgccga
     6541 cgagctggat catgaacacc ccgcacaaac cggcaaccga cgatgtccgc atcagggaaa
     6601 tcaaggagct gacgccacct tcgcgcatcc tggctgaact cacggccagc gccgccgcgt
     6661 cggacaacat cgtcgccacc cgccaggaaa ttcatcgcat cctgcatggc gcggacgacc
     6721 gtctgctggt catcatcggc ccctgctcga tccacgacca cgatgccgcc atcgaatacg
     6781 ccaaattgct gctggagcag cgcaagcgcc tgtcggccga cctgctcatc gtcatgcgcg
     6841 tgtacttcga aaagccgcgc accacagtag gctggaaagg actgatcaac gacccgcatc
     6901 tggacggcag tttccgcatc aacgaaggcc tgcgactggc gcggcgcatc ctgatcgagg
     6961 tcaacgaact cggcatgccc gccggcaacg agttcctgga cgtcattacg ccgcaataca
     7021 cggcggatgt ggtgtcatgg ggcgcgatcg gcgcacgtac cacggaaagc caggtgcacc
     7081 gcgaactagc ctccggcctg tcgtgcccgg tcggcttcaa gaacggcacc gatggcaaca
     7141 cccgcattgc cgtagacgcc atcaaggccg ccgccagtgc gcaccatttt ctgtcggtga
     7201 ccaaggccgg tcattcggcc atcgtctcca ccgccggcaa cgaggattgc cacctcatcc
     7261 tgcgcggcgg caagcagccc aactacgata ctgccagcgt ggacgccgcc tgcaaggaat
     7321 tggggattgc cggcgtagcg ccgcgcgtga tgatcgactg ttcgcatgcc aacagccgca
     7381 aggacttcag gctgcaactc gatgtcgccc gcgacgtcgc cgggcaagtc gctgccggcg
     7441 accagcgcat tttcggcctg atggtggagt cgcacctgaa ggaaggccgc caggatctgg
     7501 agcccggctg cgcgctggaa tacggcaaat ccatcaccga tgcctgcctc ggctgggaag
     7561 acagcgtgag gctgctggaa atcctggcgg aaggcgtgcg ggcacgacgt cggctgtatg
     7621 aagcggagga agaggattga ttaccaaaat tcatacaaaa accctgtcgt gcgccgtata
     7681 atgccccctc attttcagtg acttaggtag cgtagccacg atgagcatca aatccgacaa
     7741 gtggatccgc cgcatggcgg aacagcatgg catgatagcg ccgttcgagc ccggccaggt
     7801 gaaagaggcc aacggccaca agctggtgtc ctacggcacc tccagttatg gttacgacat
     7861 ccgctgctcc agcgaattca agctgttcac caacatcaac agcactatcg tcgaccccaa
     7921 gaatttcgac cccaactcct tcgtcgaagt cgatgccgac tactgcatca tcccgcccaa
     7981 ctcgtttgcc ctggcgcgca ccgtggaata cttccgcatc ccgcgcaacg tattgacggt
     8041 ctgccttggc aagtcgacct acgcacgctg cggcatcatc gtcaacgtga cgccgttcga
     8101 gccggaatgg gaaggctacg tcacgctgga attcagcaac accacgccgc tgccggccaa
     8161 gatttacgcc aacgaaggct gcgcgcaggt gctgttcttc gaggctgacg cagacgacgt
     8221 gtgcgaaacg tcctacaagg accgtggcgg caagtatcaa ggccagcacg gcgtcaccct
     8281 gccgaaaatc tgaagccgtg acgaccaggc tgctttgctg cttcgcgatg gtgatgctta
     8341 acatcacggc gacgttcgcc gacgacaagc caaagaatat cgacctctcc aatttcactt
     8401 gcaaggagtt cctgcacagc atgcagaacg gccacgagca ggaaatgcgg tcactggcca
     8461 tgtggctgga tggctacctc ggcggcatct ccggcaacac cgaagttgac tggcaggaac
     8521 tcaagcaata caccgatgag ctgatcacct attgccgcaa caaccctgcc agcgtgatgc
     8581 gcgaagcggc acgcaaagcc ggcatgtaag ccagccttca ccaggaaaaa accacatgaa
     8641 attccgcttc cccgtcatca tcatcgacga agacttccgc tccgaaaacg cctccggcct
     8701 gggcatacgc gtactggcca aggccatcga ggacgaaggc atggaagtgc tgggcgtcac
     8761 cagctacggc gacctgacct ccttcgccca gcagcagagc cgtgcttctg ccttcattct
     8821 gtccatcgac gacgaggaat tcgtcgagga gtccaagacc gcgctggaca gcctgcgcaa
     8881 gttcgtcgag gagattcgct accgcaacga ggaaatcccc attttcctgc acggcgagac
     8941 gcgcacctcg cgccacatcc ccaacgacgt gctgcgcgaa ctgcacggct tcatccacat
     9001 gaacgaggat acgccggaat tcgtggcgcg ctacatcgcg cgcgaagccc gcacctattt
     9061 ggattccctg ccgccgccgt tcttccgcgc gctgacccac tacgcggccg acggctccta
     9121 ctcctggcac tgtcccggcc actccggcgg cgtcgccttc ctgaagtcgc cggtgggcca
     9181 gatgttccac cagttcttcg gcgagaacat gctgcgcgcc gacgtctgca acgccgtcga
     9241 ggaactcggc cagttgctcg accacaccgg cccggtcgcc gcctccgagc gcaacgcggc
     9301 gcgcatcttc aatgccgacc acctgtattt cgtcaccaac ggcacgtcga cgtcgaacaa
     9361 gatcgtctgg cactcgacgg tggcgcccgg agacatcgtc gtcgtcgacc gcaactgcca
     9421 caagtcggtc ctgcattcga tcatgatgac cggcgccgtg ccgattttcc tgatgccgac
     9481 gcgcaacaac ttcggcatca tcgggccgat cccgaaggcc gagttcgcct gggagagcat
     9541 ccggaggaag atcgccgcga accccttcgc gaccgacaag aacgccaagc cgcgggtgct
     9601 caccatcacc cagtcgacct atgacgggat catctacaac gtcgaggaaa tcaagaagga
     9661 gctcgacggc cggatcgaca cgctgcattt cgacgaggcc tggctgccgc atgccgcctt
     9721 ccacgatttc tacggcgatt accacgccat cg
//
LOCUS       DAOMFA010000056         9701 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_758_length_9701_cov_4.914576, whole genome shotgun sequence.
ACCESSION   DAOMFA010000056 DAOMFA010000000
VERSION     DAOMFA010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 9701)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9701)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9701
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_758_length_9701_cov_4.914576"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(79..288)
                     /gene="rpoZ"
                     /locus_tag="PKW44_04205"
     CDS             complement(79..288)
                     /gene="rpoZ"
                     /locus_tag="PKW44_04205"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018412215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HQR50821.1"
                     /translation="MARITVDDCLERIPNRFHLTLAAAYRARQVANGSEPLVSGAQKD
                     KPTVVALREIAAGKVGLEILNRGQA"
     gene            complement(314..937)
                     /gene="gmk"
                     /locus_tag="PKW44_04210"
     CDS             complement(314..937)
                     /gene="gmk"
                     /locus_tag="PKW44_04210"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012777550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HQR50822.1"
                     /translation="MVPVKTSGNLFIVTAPSGAGKTSLVRALLAADSQVRLSVSYTTR
                     PMRPGEVGGLDYHFVTEEHFLKMLEEGDFLESAEVHGARYGTSQSTVETQLREGHDLV
                     LEIDWQGAAQVRGLHPNAINIFILPPSMEVLAQRLNRRGQDSQEVIARRLAGARAEIS
                     HVGEFDYVIINDQFETAIHDLLAIVRSQRLRVEKQLRRHQDLLQKFS"
     gene            complement(1001..1738)
                     /locus_tag="PKW44_04215"
     CDS             complement(1001..1738)
                     /locus_tag="PKW44_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR50823.1"
                     /translation="MAETFWPRFHKYGVLLCAAWLAACAAPAPPFKGKEVPQEGDRSS
                     FSQLGKTDIDRMADVEMRENTQSLQLLMTKLYKRNPRELKKSTSGTVEEMVGWVFEGD
                     HGWKFKRINEVQDTEALYLSFSPDYEGDRVLPFIVGLYTMMLKAHGGKTEFFLMDSID
                     PQSVYNAARNVEIADWKLTNARDDKGQLYLLTNELSETERNLSFEREFGKIVGRMDVF
                     AMTLAEKKQRFITRATHNVATALFLPF"
     gene            complement(1750..2616)
                     /locus_tag="PKW44_04220"
     CDS             complement(1750..2616)
                     /locus_tag="PKW44_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HQR50824.1"
                     /translation="MIYSMTGFSAQQREAAGGTLLLELRSVNHRYLELQLRLDETLRG
                     FETSIREVIAGRLGRGKVECRLSFTAASGAASAAQLDNAVLEQVALLGAAVQQRFPQS
                     QPLSVADILRWPGVLTGAEVSADKLASEVIALVEQGLQDLAEARAREGEKLKALILER
                     AAAMETQVAKVRPLLPALMQAYQDKLAARLREALQSVDEERVRQELTLFAQKIDVDEE
                     LSRLTTHLQEVKRILSAGGAVGKRLDFLMQELNREANTLGSKSASTEVSQVSMELKVL
                     IEQMREQIQNIE"
     gene            2868..3815
                     /locus_tag="PKW44_04225"
     CDS             2868..3815
                     /locus_tag="PKW44_04225"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011310987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HQR50825.1"
                     /translation="MGKRDQKHQALPAGYQLLDYKIDKVLNSGGFSFVYLAHDAQGNV
                     VAIKEYLPDKLASRGGDSRVHVSSPDTLVNFRIGLRSFFEEGRALAHINHRNVVRVLN
                     FFRANETVYMVMQYERGKTLQEYILLHPRPVKEVFVRRMFGELLSGLREVHTHKLLHL
                     DIKPTNIYLRHDGSPLLLDFGSVRQTLTDARPKLPPCYTPGFAAPEQYVNRKLLGPWS
                     DIYSIGASIYACLIRSTPVAADQRLKEDTLQPATKIGRGIYSQQLLETVDWCMQLDHM
                     KRPQSVLTLQKALLDYSPEKPRKKSLLTELREKLTKAIV"
     gene            3823..4758
                     /locus_tag="PKW44_04230"
     CDS             3823..4758
                     /locus_tag="PKW44_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012777542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HQR50826.1"
                     /translation="MKFTIFQASRNGPRPYNHDRVAYSYSKESLLMVLADGLGSNPHG
                     GLAAKIAVKTLTDAFQQTARPDIANPSKFLDDQIRQVHSTIQRYAVANELYELPRTTV
                     VAAIVQHDWLHCVHAGDSRLYHFRDGGLLYRTVDHSKVQLMHDKGEIEAEEMVAHPER
                     NKIYSCLGGDIPPRIEIMRKRILLGGDIVLLCSDGLWAMVNDREFCDTLERENVTRSV
                     PVLLNLAESRCSNGGDNMSAVAVNWGGHSGYPLSVSTVTMPMGLTTTIHNPVPQDASE
                     TLATTGARLSEEDIDRAISEIQAAIQKNPNKPDGL"
     gene            4785..5501
                     /gene="rph"
                     /locus_tag="PKW44_04235"
     CDS             4785..5501
                     /gene="rph"
                     /locus_tag="PKW44_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013440872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HQR50827.1"
                     /translation="MRPSLRAPGELRPVEIIRRYTKHAEGSVLVKFGDTHVICTASID
                     EKVPPFLRGKGQGWVTAEYGMLPRSTGSRMDREAARGKQSGRTQEIQRLIGRSLRSVI
                     DLDKLGERTVQIDCDVIQADGGTRTASITGGFVALHDAVSTLLEKQLLAESPLRDFVA
                     AISVGVFEGTPVLDLDYIEDSGCDTDMNVVMTGSGGFVEVQGTAEGAPFQRSEMDAML
                     GLAQAGIAELISRQKVALGL"
     gene            5503..6096
                     /gene="rdgB"
                     /locus_tag="PKW44_04240"
     CDS             5503..6096
                     /gene="rdgB"
                     /locus_tag="PKW44_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HQR50828.1"
                     /translation="MQKLVIATGNAHKLHEISTLLQPLGIKVWSQSAFSVPEAAEPHC
                     TFIENALAKARHAARLTGLPALADDSGLCVEALGGAPGVISAIYAGEPRSDARNNEKL
                     LAAMQGITDRRAHYHCALVLLRHADDPQPIIAEGQWHGVILAAARGSKGFGYDPLFLD
                     ATLNQTVAEISTENKNRVSHRGRALASLLEKLRGAPL"
     gene            6093..7244
                     /gene="hemW"
                     /locus_tag="PKW44_04245"
     CDS             6093..7244
                     /gene="hemW"
                     /locus_tag="PKW44_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HQR50829.1"
                     /translation="MIQFEQPPPLSLYIHIPWCVRKCPYCDFNSHEARQNIPEAQYVD
                     ALIRDLEQSVPQVWGRRVHSIFFGGGTPSLFSAEAIDHILSSVRALLPLEYEAEITLE
                     ANPGTVEAARFTGFRDAGVNRLSLGIQSFNDNHLKALGRIHDASEAHRAVELALATFD
                     NVNLDLMYALPGQIYEQALTDLRTALGYQPQHLSAYHLTLEPNTPFHRNPPALPDDDT
                     SAAMQEMLEQELAMHGYGHYETSAFSLKGRRCRHNLNYWQFGDYLGIGAGAHGKLSFH
                     DKVVRQARPRHPNAYLKAVEAGNGAEQEWEVTRQDIGFEFMMNALRLTEGFPVTLFQQ
                     RAGLQISVVDAALNEAERRGLLARNHLQIEPTLQGQRFLNELLQLFLPA"
     gene            complement(7257..8639)
                     /gene="argH"
                     /locus_tag="PKW44_04250"
     CDS             complement(7257..8639)
                     /gene="argH"
                     /locus_tag="PKW44_04250"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HQR50830.1"
                     /translation="MQEKSKAWSGRFSEPVAELVKKYTASVGFDQRLAEFDIQGSLAH
                     AHMLGAQGIIGADDVTAIERGLAEILAEIRAGKFEWSLDLEDVHLNIEKRLTDKVGDA
                     GKRLHTGRSRNDQVATDVRLWLRTTVDDVLAGIRKLQKSILDLAEQHADTVMPGFTHL
                     QVAQPVTFGHHLMAYYEMLKRDAGRFADARKRINYLPLGAAALAGTSYPIDREAVARE
                     LGFDGVCENSLDAVSDRDFAIEFTSDAALVMTHLSRFSEELILWSSPRFGFVDIADRF
                     CTGSSIMPQKKNPDVPELVRGKTGRVNGHLIALLTLMKAQPLAYNKDNQEDKEPLFDT
                     ADTLLVTLEIYADMLRGVNVDKAAMRNAAAEGFATATDLADYLVKKGLPFRDAHEAVA
                     RVVRHAVDKGCDLADLALADLQAFSPLVAEDIFGVLTLEGSLASRNHVGGTAPEQVRK
                     AIARARKTLD"
     gene            8670..9689
                     /locus_tag="PKW44_04255"
     CDS             8670..9689
                     /locus_tag="PKW44_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HQR50831.1"
                     /translation="MASIKQNLQPFPVSLPDFRNMGVNVRIMLLAIGMTVIATALRAQ
                     TPERTWLELVNLSSAIQPLLLLSLLVLYALNAKLSRLPYAWGVAAVFAVELALVCLSR
                     QLALRSLGEIEVAPLWHFLLFTAIMTGVTLFYFNLRYRALSPAITEARLQALQARIRP
                     HFLFNSINAVLSLIRSDPKRAETALEDMADLFRVLMADNRKLVPLHSELDLCRQYLAL
                     EKLRLGERLQLAWHIDDMPGDALIPPLLLQPLLENAVYHGIEPLHAGGEIDIRIYRNR
                     NEVHLVLTNPYHKESDHHNGNNMALSNIRERLSLHFDAEAQLTTHVTQDQYQIHIVIP
                     YREAA"
BASE COUNT         2003 a         3053 c         2737 g         1908 t
ORIGIN      
        1 atccatgtcg agtggcagca cgggatgcgg cgtagttacc gcagccttca gcgcgttgga
       61 ttggccgccc atcgcatttt acgcttgccc gcggttgagt atttccaacc ccaccttgcc
      121 ggcggcgatt tcgcgcaggg cgaccacggt cggcttatcc ttctgcgcgc cactgaccag
      181 cggctcggaa ccgttcgcca cctggcgtgc gcgataggct gctgccaagg tcagatgaaa
      241 gcggttgggg attctttcca gacaatcgtc tacggtaata cgtgccatta ggtaatcctt
      301 tatcgatgaa tttctagctg aatttctgca gcaaatcctg atgccgccgc agctgctttt
      361 ccacacgcag gcgctggctg cgaacgatag cgagcagatc gtgaatcgcc gtctcaaact
      421 ggtcgttgat tataacatag tcaaattcgc cgacatggct gatctcggca cgcgcgccgg
      481 ccagacggcg cgcgatgact tcctggctat cctgaccgcg ccggttcagc cgctgcgcca
      541 gcacctccat cgaaggaggc agtatgaaga tgttgatggc gttgggatgc agcccgcgca
      601 cctgcgctgc gccctgccag tcgatttcca gcaccagatc gtggccttcg cgcagttgcg
      661 tttcgaccgt gctctgcgaa gtaccgtaac gcgcaccgtg cacttccgcg ctctccagga
      721 aatcgccctc ctccagcatc ttgaggaaat gctcttcggt gacgaaatga taatccagcc
      781 cgcctacctc gcctggacgc atcggccgcg tggtgtagga aacagacagc cgcacctggc
      841 tgtcagctgc cagcagggcg cgcaccaagc tggtcttgcc ggcgccggaa ggagcggtga
      901 cgataaagag gttgccggac gttttcacag gaaccatgtc gttgaattga acagcgctga
      961 ttttcaccca attcccgtgc ggacggaagc cctgccaccc tcagaacggc aaaaacagcg
     1021 ccgtcgccac gttatgcgtc gcacgggtga tgaagcgttg tttcttttcc gccagcgtca
     1081 tggcaaacac atccatacga ccgacgatct tgccgaattc gcgctcgaaa ctaaggttgc
     1141 gctcggtctc gctcaattcg ttggtcagca aatagagttg ccccttgtcg tcacgcgcgt
     1201 tggtaagctt ccagtcggca atctcgacat ttcgcgccgc gttgtaaaca ctctgcgggt
     1261 ctatgctgtc catcaggaag aactccgtct tgccgccgtg cgccttgagc atcatggtat
     1321 acagtccgac gatgaacggc agcacccggt ctccctcgta gtcgggagaa aacgaaagat
     1381 aaagcgcttc cgtgtcttgc acctcattga tgcgcttgaa tttccagccg tggtcgccct
     1441 cgaacaccca gcctaccatc tcctccacgg tgccggacgt gctcttcttc aactcacgcg
     1501 ggttgcgctt gtacagcttg gtcatcagca actgaaggct ctgcgtgttc tcgcgcattt
     1561 cgacatcggc catgcggtcg atgtcggtct tgcccaactg gctgaatgaa ctgcggtcgc
     1621 cctcctgcgg aacctccttg cccttgaacg gtggcgcggg agcggcgcag gcagccagcc
     1681 atgcggcgca tagcagtacg ccatatttgt ggaaccgagg ccagaaagtc tctgccatgt
     1741 tgccgcgggc tattcgatgt tctggatctg ttcgcgcatc tgctcgatca acaccttgag
     1801 ttccatcgac acttgggaga cttcggtgga ggccgacttg gagcccaggg tattggcctc
     1861 gcgattgagc tcctgcatca ggaaatccag ccgcttgccg accgcgccgc cggcggagag
     1921 gatgcgtttg acttcctgca aatgggtggt gagacgggaa agctcttcgt cgacgtcgat
     1981 cttctgcgcg aacagcgtca gttcctggcg gacacgctcc tcatccacac tttgcaatgc
     2041 ctcgcgcaac cttgctgcca gtttgtcctg ataggcctgc atcagggcgg gcagcaacgg
     2101 ccgtaccttg gctacttgcg tttccatggc cgctgcgcgc tcgagaatca gcgccttcag
     2161 cttctcacct tcgcgagcgc gtgcctctgc cagatcctgc aacccctgct cgaccagagc
     2221 aatgacttct gacgccagct tgtcagcgct cacctctgcg cctgtcagca cgcccggcca
     2281 gcgcaggata tccgccacgc tcaacggctg gctttgcgga aagcgttgct gcacagcggc
     2341 accgagcaac gcaacctgct ccagcaccgc gttgtcgagc tgtgccgcag aagctgcacc
     2401 agacgctgcc gtgaacgaaa ggcggcattc caccttgccc cgccccagcc gaccggcaat
     2461 gacctcacga atggatgttt cgaaaccacg caacgtctcg tccaggcgca attgcagttc
     2521 gaggtagcgg tgattgaccg agcgtagttc cagtaacagt gtgccacccg cagcctcgcg
     2581 ctgttgtgcg gaaaagccgg tcatgctgta aatcatgccg tttatctcct aattttcatc
     2641 catgttatca aatccgtaat ggttcatgac agaatgcacc agtagtaaat ttcagtgtcc
     2701 ttaaaatttc aggtttccga acaaggtacg acacgagcaa aaaattgcaa cagcgcatat
     2761 agtaaccaac tgaaaaacgt tgaaactgaa tacttggcaa gttctgaccc cagggattga
     2821 cttgaggtta tccacaattc aattgtattt ggagagacgc gcaagatatg ggaaagcggg
     2881 accaaaaaca tcaagccctg ccagccgggt accagttgct ggactacaag atcgacaagg
     2941 tcttgaacag cggcgggttc agctttgttt atctggccca tgacgcccag ggaaatgttg
     3001 ttgccatcaa ggaatacttg ccggacaagc tggcttcgcg cggcggggat tcgcgggtcc
     3061 atgtcagctc cccagacacg ctcgtcaatt tccgcatcgg actgagaagt tttttcgagg
     3121 aagggcgtgc gctggcccac atcaaccatc gcaacgtggt gcgggtgctc aattttttcc
     3181 gtgccaacga gacggtttac atggtcatgc agtacgagcg cggcaaaacc ttgcaggaat
     3241 acattctcct gcacccgagg ccggtgaagg aagtcttcgt gcgccggatg ttcggcgaac
     3301 ttttgagcgg tttgcgcgaa gtacataccc acaagctgct gcatctcgac atcaaaccta
     3361 ccaacatata cctgcgtcat gatggctctc cgctactgct ggatttcggt tcggtacggc
     3421 agacgctgac cgatgccaga cccaagctgc cgccctgcta cacacccggc tttgcggcac
     3481 cagaacagta tgtcaaccgc aagttgctgg ggccgtggag cgacatctac agcatcgggg
     3541 cgtcgatcta cgcctgcctg atacgcagca caccggtcgc cgcagaccag cggctaaagg
     3601 aggataccct gcagcccgcc accaaaatcg ggcgaggcat ctattcccag caactgctgg
     3661 agactgtgga ctggtgcatg cagcttgacc atatgaaacg tccgcagagc gtgctgacat
     3721 tgcagaaagc cctgctggat tattccccag aaaagccgag aaaaaaatcc ttgctgaccg
     3781 agttgcgcga aaaattgacc aaagcgatcg tctgagccaa gaatgaagtt cactattttt
     3841 caggccagcc gcaatggtcc acggccttac aaccatgacc gggtggctta ttcctacagc
     3901 aaggaatcat tattgatggt gctggctgac ggcttgggca gcaaccccca tggtgggctt
     3961 gccgccaaaa tcgccgtcaa gacgctcact gacgccttcc agcagacagc acgacctgac
     4021 atcgccaatc catccaaatt tctcgacgac caaatccgcc aggttcatag cacgatccag
     4081 cgctatgcgg tggccaatga gttgtacgaa ttgccgcgca ccaccgtcgt ggcggctatc
     4141 gtgcagcatg actggctgca ttgcgtccac gccggagact ccaggcttta ccacttccgc
     4201 gatggcggcc tgctataccg taccgtagac cattccaagg tacaactgat gcacgacaag
     4261 ggtgaaatcg aagcggagga aatggtcgcg cacccggagc gaaacaaaat ctacagctgc
     4321 ctgggtggcg acatcccgcc caggatcgag atcatgcgca agcgcatatt gctcggcggc
     4381 gacattgttc tgctttgctc cgatggcttg tgggccatgg taaatgaccg agagttttgc
     4441 gacaccctgg aacgcgaaaa cgtgaccaga tccgtgcccg tcctgctcaa cctggccgag
     4501 tctcgatgca gcaacggcgg cgacaacatg agcgccgtcg cagtcaattg gggaggacat
     4561 tcgggatacc cgctctccgt ttccaccgtc accatgccta tgggtcttac caccacgatc
     4621 cacaatcccg tcccgcagga cgcgagcgaa acccttgcta caactggcgc caggctctcg
     4681 gaagaggata tcgacagagc catttcggaa atacaggcgg ctattcagaa aaacccgaac
     4741 aagcctgacg gtttataatc cgcttttttc tgtcaggaaa catcatgcgt cccagcctac
     4801 gcgcgcccgg ggaattgcgc ccggtcgaaa tcatccgccg ctacaccaag cacgccgaag
     4861 gttcggtgct ggtaaagttc ggcgacaccc atgtcatctg caccgccagc atcgacgaaa
     4921 aagtcccacc cttcctgcgc ggtaagggcc aaggctgggt caccgctgaa tacggcatgc
     4981 tgccgcgctc caccggcagc cgtatggatc gcgaggcggc acgcggcaag caatccggac
     5041 gcacgcagga aatccagcgc ctgatcggac gcagcctgcg ttctgtcatt gacctcgaca
     5101 agctgggcga acgcaccgtc cagatcgact gcgacgtcat ccaggccgac ggtggcaccc
     5161 gcaccgccag catcaccggc ggcttcgtcg ccctgcacga cgcagtaagc accctgctgg
     5221 aaaagcaatt gctcgccgaa tccccgctac gcgacttcgt cgccgccatc tcggtcggcg
     5281 tgttcgaggg cacgccggta ctggacctgg actacatcga ggattccggc tgcgacaccg
     5341 acatgaacgt cgtcatgacc ggcagcggcg gtttcgtcga ggtgcagggc acagcggaag
     5401 gtgcgccgtt ccagcgttca gagatggatg ccatgctggg cctggcccaa gccggcatcg
     5461 ccgaactgat ctccaggcag aaagtcgcac tgggtctgta gcatgcagaa actggtgatc
     5521 gccaccggca acgcccataa gctgcacgaa atcagcaccc tgctgcaacc gctgggcatc
     5581 aaagtatggt cgcaatcggc cttcagcgtg ccggaggctg ccgagccgca ctgcaccttc
     5641 atcgagaacg cgctggccaa ggcacgccac gccgcccgcc tcaccggcct gccggcgctg
     5701 gccgacgatt ccggcctgtg tgtagaggca ttgggcggcg cgccaggcgt catttccgcc
     5761 atctacgccg gcgagccgcg ctccgacgcg cgcaacaacg aaaaactgct ggcggcgatg
     5821 caaggcatca ccgaccgccg cgcgcattac cactgcgcgc tggtgctgct gcgccatgcc
     5881 gacgacccgc aacccatcat cgccgaaggc cagtggcatg gggtaatcct tgctgccgcg
     5941 cgcggcagca agggcttcgg ctacgacccg ctgtttctcg acgccacgct aaaccagacc
     6001 gtggccgaga tttccaccga gaacaagaac cgcgtcagcc atcgcggccg cgcgttagcg
     6061 agtctgctgg aaaaactgcg cggcgcgccg ctttgatcca attcgagcaa ccgccgccgc
     6121 tgagtctcta catccatatc ccgtggtgcg tgcggaaatg cccgtactgc gacttcaact
     6181 cgcacgaggc gcggcaaaac attcccgaag cgcagtacgt tgacgcgctg atccgtgacc
     6241 tggagcaatc ggtaccgcaa gtgtgggggc ggcgcgtgca cagcatcttc ttcggcggcg
     6301 gcacgcccag cttgttcagc gccgaagcca tcgaccacat cctcagcagc gtgcgcgccc
     6361 tgctgccgct ggaatacgag gccgagatca cgctggaagc caaccccggt acggtggaag
     6421 ctgccaggtt caccggtttc cgtgatgccg gggtaaaccg gttatcgctg ggcatccaga
     6481 gcttcaacga taaccatctc aaggccctcg gccgtatcca cgacgcgagc gaagcgcacc
     6541 gcgccgtgga gcttgcgctg gccacgtttg ataacgtcaa ccttgacctg atgtacgccc
     6601 tgcctggcca gatttatgag caagcgctga ctgacttgcg taccgcactc ggctaccaac
     6661 cgcaacacct ctccgcctac cacctcaccc tcgagcccaa caccccgttc caccgcaacc
     6721 cgccggcgct gcccgacgac gataccagcg cggccatgca ggaaatgctg gaacaggaac
     6781 tggccatgca cggctacggc cattacgaga cttcggcgtt ttccctcaaa ggccgccgct
     6841 gccgccacaa cctcaactac tggcagttcg gcgactacct cggcatcggt gcaggcgccc
     6901 acggcaagct gtcgttccac gacaaggtcg tgcgtcaagc tcggcccagg catcccaatg
     6961 cctacctgaa agcagtggaa gccggcaatg gcgccgagca ggaatgggaa gtcacgcgcc
     7021 aggacatcgg cttcgaattc atgatgaatg cgctgcggct aaccgaaggc ttcccggtga
     7081 ctttgttcca gcagcgcgcc gggttgcaga tcagcgtagt ggatgcagcg ttgaatgagg
     7141 cggaaaggcg cggcctgctg gcacgcaacc atctgcaaat cgagccgacg ctgcaagggc
     7201 agcggttttt gaacgagttg ctgcagttat ttttgcccgc ctgatggcaa gctggcttaa
     7261 tccagcgttt tccgcgccct ggcaatcgcc ttcctgacct gctccggcgc agtgccaccg
     7321 acgtggttac ggctggcgag agagccttcg agcgtcagca ccccgaagat gtcctcggct
     7381 accagcggcg agaaggcttg caggtcggct agcgccaggt cggccaagtc gcagcccttg
     7441 tccacggcat ggcgcaccac ccttgccacc gcttcgtgag catcacggaa tggcaggcct
     7501 ttcttgacca ggtagtcggc caagtcggtg gcggtggcaa agccttccgc cgcggcgttg
     7561 cgcatggcag ccttgtccac gttcacgccg cgcagcatgt cggcgtagat ttccaaggtc
     7621 accagcaggg tgtcggcggt gtcgaacagc ggctccttgt cttcctggtt gtccttgttg
     7681 taggccagcg gctgcgcttt catcagcgtc agcagggcga tcagatggcc gttgacgcgg
     7741 ccggtcttgc cgcgcaccag ttccggcacg tcagggttct ttttctgcgg catgatggag
     7801 gaaccagtgc agaagcggtc ggcgatatcg acgaagccga aacgcggcga cgaccataaa
     7861 atcagctctt cggaaaaacg cgagaggtga gtcatcacta gcgctgcgtc gctcgtgaat
     7921 tcaatggcaa agtcacggtc cgagacagca tccagcgagt tttcgcacac gccgtcgaag
     7981 cccagttcgc gcgctacggc ttcgcggtcg atgggatagc tggtgccggc cagtgcagca
     8041 gcgcccaggg ggaggtagtt gatgcgcttg cgtgcgtctg caaagcgtcc ggcatcgcgc
     8101 ttgagcatct cgtaataggc catcaggtgg tggccaaagg tgaccggctg ggctacttgc
     8161 aaatgggtga agccgggcat cacggtatcg gcatgctgtt cggcgagatc gaggatggat
     8221 ttttgcaact tgcggatgcc agccagcacg tcatccacgg tggtgcgcag ccacaggcgc
     8281 acatcggtcg ccacctggtc gttgcgcgag cggccagtgt gcagacgttt gccggcatcg
     8341 ccaaccttat ccgtcagccg tttttcgatg ttaagatgca cgtcctccaa gtcgagcgac
     8401 cattcgaatt tcccggcacg gatttccgcc aggatttcgg ccaaaccgcg ctcgatggcg
     8461 gtcacgtcat ccgccccgat gatgccctgc gcgcccagca tgtgagcatg ggccagcgaa
     8521 ccctgaatgt cgaactcggc caggcgctgg tcgaatccca ccgaagccgt gtattttttc
     8581 accagttccg ccaccggttc actgaaacgg ccggaccaag cctttgattt ttcttgcata
     8641 aggaaagcgc ttattcataa aattgaatca tggcaagtat aaagcaaaat ctccaaccct
     8701 ttcccgtgag tctgccggat ttccgcaaca tgggtgtcaa cgtgcgcatc atgctgctgg
     8761 cgatcggcat gaccgtcatt gccaccgcgc tgcgcgcgca gacgccggag aggacatggc
     8821 tggaactggt caacctgagc tcggccatcc agccgttgct gctgctcagc ctgctggtgc
     8881 tgtacgccct caacgccaaa ctctcccgcc tgccttacgc ctggggcgtg gccgcggttt
     8941 tcgccgtgga actggcgctt gtctgcctgt cacgccagct ggcgctgcgc tctctgggcg
     9001 aaatcgaagt ggcgccgctt tggcatttcc tgctgtttac cgccatcatg accggcgtta
     9061 ccctgttcta tttcaacctg cgctaccgcg cgctgtcgcc ggccatcacc gaggcccgcc
     9121 tgcaggcgct acaggcgcgc atccggccac actttttgtt caacagcatc aacgcagtgc
     9181 tatcgctgat ccgcagcgat cccaaacgtg ccgaaactgc tctggaagac atggccgacc
     9241 tgttccgcgt gctgatggcg gacaaccgca agttggtacc gctgcacagc gagctcgacc
     9301 tgtgccggca atatctggcg ctggaaaaac tgcgcctggg agagcgtctg caactggcgt
     9361 ggcacatcga cgacatgcct ggcgacgcgc tgattccgcc gctgctactg cagccgctgc
     9421 tggaaaacgc cgtttaccac ggcatcgaac cgctccatgc cggcggcgag atcgacattc
     9481 gcatttaccg caaccgtaac gaggttcacc tggtgctgac caacccgtat cacaaggaaa
     9541 gcgaccacca caacggcaac aacatggcat tatccaacat ccgcgagcgg ctctccctgc
     9601 attttgatgc cgaggcgcag ctgacgaccc acgtgacgca agaccagtac cagatccata
     9661 tcgtcatccc ttatcgggaa gccgcatgag cattgccccc c
//
LOCUS       DAOMFA010000057         9643 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_768_length_9643_cov_5.105236, whole genome shotgun sequence.
ACCESSION   DAOMFA010000057 DAOMFA010000000
VERSION     DAOMFA010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 9643)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9643)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9643
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_768_length_9643_cov_5.105236"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..339
                     /locus_tag="PKW44_04260"
     CDS             <1..339
                     /locus_tag="PKW44_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HQR50832.1"
                     /translation="NGSRQQADEFIKLGFKLGFGGAMTWPRALRIRELAAMLPLEAIV
                     LETDAPDMPPEWLGSGGRNNPQQLPHIAQTLAELRRVNVGEIIDATARNVVVALPKLP
                     QLFTSPHVLL"
     gene            529..1602
                     /gene="mtnA"
                     /locus_tag="PKW44_04265"
     CDS             529..1602
                     /gene="mtnA"
                     /locus_tag="PKW44_04265"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HQR50833.1"
                     /translation="MKIDGKDYRTIWFVDGGKAIEVIDQTLLPHRFAILRLGTLAEVA
                     HAIKNMNVRGAPLIGAAASCGIALAMREESDDATLAAAVDLLCATRPTAVNLHWAVER
                     MLRALRPLPPARRAEVAITEAAAICEEDVILNHGIGQHGLKLLQQHYAERQRPLNLLT
                     HCNAGWLATVDWGTALAPVYAAFDAGIPLHVWVDETRPRNQGAALTAWELGKHGVPHT
                     VITDNAGGHLMQHGKVDAVIVGADRVTSRGDVCNKIGTYLKALAAHDNGIPFYAAVPS
                     PSIDWRMADGLSEIPIEVRSAKEVAWIQGRLSNGEIAQVNLLPDGSTAANPAFDVTPS
                     RLVTGIITEHGVIHPDQLRSLFP"
     gene            1599..2243
                     /locus_tag="PKW44_04270"
     CDS             1599..2243
                     /locus_tag="PKW44_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HQR50834.1"
                     /translation="MTHLREQLLAVTRKLSELGLNRGTAGNASVRDGTGFLVTPSGMS
                     VEAMTPHDMVWVDFDGNAQGERKPSSEWRFHRDILHARPEVGAVIHTHAMFATTLACL
                     RRDVPPFHYMIAVAGGDSLRCAPYALFGTQALSDAALTALQDRKACLLANHGMIAVGR
                     DLEAALAMTQEVETLCEQYWRTLQVGGPTLLTPQEMDEALEQFKDYGRWRQESQ"
     gene            complement(2206..3600)
                     /locus_tag="PKW44_04275"
     CDS             complement(2206..3600)
                     /locus_tag="PKW44_04275"
                     /inference="COORDINATES: protein motif:HMM:NF017591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF839 domain-containing protein"
                     /protein_id="HQR50835.1"
                     /translation="MRFLILLCYLMCSLSLQAAPSVPYLHPLIGDAEVKPLLTVGEAA
                     GNGYRLAGVPDGLGALDNGDGTLTVLMNHEIPADKGVPRAHGGKGAFVSRWTIRLADL
                     AVLRGEDLVRQVQLWDAVSASHNVVPAVAFGRFCSADLAADPAFTGSEGEGFDGRLLL
                     NGEEEKDAGGRAFAHVVGGAQAGVSYELPHLGRVAWENLLALPQSGHRTVVVGMDDYP
                     GGHVYVYVGEKRRTGNPVEKAGLVGGALYAVRFEDRRFSLLPLGEVVGLDGKVLRARA
                     RQLGATPLLRPEDGAWDKRDRNVFWFATTDRIDGKSQLFRLAFDDSQHPEQGGHVDEV
                     LEAGEVGAQMFDNLAVDGDGRVLIEEDPGESDRLAAIWLYDPARPHTAQKLFESESRL
                     FTNGGAEFMTEDEEHSGIIEVTGLLKSAPWAKPDRRYFFGVTQAHTPQQDAELVEGGQ
                     LWLIAIPAASVRSP"
     gene            complement(3719..5230)
                     /locus_tag="PKW44_04280"
     CDS             complement(3719..5230)
                     /locus_tag="PKW44_04280"
                     /inference="COORDINATES: protein motif:HMM:NF019042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HQR50836.1"
                     /translation="MKSIKLRGVVASVSGAMLLGALPAMADSNDDIINALMAKGVLTE
                     EEGALLMKGRQGEKEAAEAKKKSEIKASFKDGIVFESGDKAFKMSINGRVQADYRTFH
                     PQQVNNQADTWDIRRAYLGVKGTFYDWIDFAAIANFANTSSQNSSAMYEYYLNLRPVD
                     YAQLQMGQFKQPMSLEELTSSRFINFTERSYAAQLAPGIDRGAMLQGIPVKGTTYAVG
                     YFNGLGSNINSSAQNFNENSIQNDGKAWTGRVTANIAELMGNPDMVAHLGVAYSDSWG
                     NNNTSGTANSTQVTLRSEGRGLDVFKSANLRDGFDLTRTGLEGAFAYNQFKLQSEYAH
                     ADYQNDKGLIVSGTATDDSKTIKTWNAALTWLVTGEKYADSYKNGKFDRIKPFNDFNP
                     RMEGGNKGWGAWELGLRYTNLDASDFETPYLAATVGPTPAAQTAKFNEAHAWTAGVKW
                     IINPNTRFLLDYIHTKLDCANGTTTAQTNANKAACDSVDDTENAMNFRAQFDF"
     gene            complement(5493..6080)
                     /locus_tag="PKW44_04285"
     CDS             complement(5493..6080)
                     /locus_tag="PKW44_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HQR50837.1"
                     /translation="MLSKQNIFKRNTEPSGAIPAKPISPAPLGSARPVSTSTLPGYPA
                     ESTPTVTTAPVSTEAKPAEAPKADQPSGSRLIVGPEVKLKGAEILNCDTLVVEGRVEA
                     TMDSRVIQIAAEGAFSGKVGIDVAEIHGKFDGELTARSQLIIHSTGKVSGKIRYGKIL
                     IEEGGELSGDVAAFTPDSSKAGGLRIAGQASAIEP"
     gene            complement(6323..6532)
                     /locus_tag="PKW44_04290"
     CDS             complement(6323..6532)
                     /locus_tag="PKW44_04290"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HQR50838.1"
                     /translation="MEKITLHVSGMACGGCASAIAQALLAQPGVVEAEVSHIEATAVI
                     AYDPARVQPEQLKMTVEQAGYKVVD"
     gene            complement(6534..8033)
                     /gene="lysS"
                     /locus_tag="PKW44_04295"
     CDS             complement(6534..8033)
                     /gene="lysS"
                     /locus_tag="PKW44_04295"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HQR50839.1"
                     /translation="MTEDQIPQDESHVIAERRAKLKAMREQGVAFPNDYRRSHLAADL
                     HAAHGEKPGEELEPHGIRVSVSGRMMLKRVMGKASFATIQDPSSRIQLFIARDNVSEE
                     VYDAFKKWDLGDIVAAEGVLFKTKTGELSVKVDALRLLTKSIRPLPEKFHGLSDQETK
                     YRQRYVDLIVSEDTRKTFVTRSKIVAAIRHFMLDHQFLEVETPMLHPIPGGAAAKPFK
                     THHNALDMQMFLRIAPELFLKRLIVGGFERVFEINRNFRNEGLSVRHNPEFTMMEFYA
                     AYTDYQWLMDFTEQCIRAAAIAAQGTATVIYQGREVDLSMPFERLTIVGAVRKYAPQY
                     TDAQLADSAFLRTELKRFGIDALPHLGLGALQLALFEETAEAQLWQPTYIIDYPVEVS
                     PLARASDQRPGITERFELFITGREIANGFSELNDPEDQAARFRQQVEAKEAGDDEAMY
                     YDADYIRALEYGMPPTGGCGIGIDRLVMLLTDSPSIRDVILFPHMRPEA"
     gene            complement(8058..8489)
                     /locus_tag="PKW44_04300"
     CDS             complement(8058..8489)
                     /locus_tag="PKW44_04300"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HQR50840.1"
                     /translation="MIYFDTSALVKRYVPERNSHAADLYFVENAPVAVSRLVLLELRC
                     TVARKRRNGEISSAAEQGVMDKARLDVQDGVLVIHPMTDAYFVEAMRLIEDYPQFALR
                     TLDATHLAAAHAIGVKELATADAVMRGVAQAMGMQVVYFGS"
     gene            complement(8492..8737)
                     /locus_tag="PKW44_04305"
     CDS             complement(8492..8737)
                     /locus_tag="PKW44_04305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR50841.1"
                     /translation="MKTLGVRQIRASYDVLVETLAAEGEVVLTHHGKPFARVLPFEEA
                     AVATRPKARSMAAFRDTMPFQEVPSEVLIREDRDARG"
     gene            complement(8801..>9643)
                     /locus_tag="PKW44_04310"
     CDS             complement(8801..>9643)
                     /locus_tag="PKW44_04310"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HQR50842.1"
                     /translation="AALLEADRLEQDAARDFLHGLGLAAPDEAKRRKAFAVRWSDCER
                     WGMEWNQQRISGTSPDVGKFPTVALGEAIDLVQYGTSEKANQDGNGLPVLRMNNVKAG
                     LLDVSDLKHINLPPKVAQGLLLKDGDILVIRTNGSRDLVGTCAVFHEVGEYVFASYLI
                     RLCVNASKLNPDFAAFFLNSALGRSQVNAVSRQIMQNNINSEELRGLRLPLPPLETQQ
                     TLVNAITAARQQTAAKRAEAGSLKTRAAAEIEAAILGESSLNNRRGRLPDDFRFDRDE
                     ANTR"
BASE COUNT         1955 a         2918 c         2755 g         2015 t
ORIGIN      
        1 aacggcagcc gtcagcaagc agatgaattc atcaagctgg ggttcaagct gggcttcggt
       61 ggtgccatga cgtggccgcg cgcgctacgc atccgggaac tggcggcgat gctgccactg
      121 gaagccattg tgctggaaac cgatgcgccg gacatgcccc cggaatggct gggatccggt
      181 ggacgcaaca atccgcaaca gctaccgcat attgcgcaga cattggcgga attaagacgg
      241 gtgaatgtgg gtgaaattat tgacgcaacg gcgcggaatg tggttgtggc gctgccaaaa
      301 ctgcctcagt tattcacatc acctcatgtg ttactttaac aaatgccaaa aaaccctagc
      361 cattcaaggg tgtgttgtaa gttattgatt tttaagtaat tgaaaattag ttcaattttt
      421 aggcgtttgt aaaaattcgt tatgccagct tgagttacat tttcaagtca cagatattca
      481 cagaattatc cacagatatt gtggattgag atatggatga ttttttaaat gaaaattgac
      541 ggaaaagatt accgaacgat ctggtttgtg gacggtggga aagccattga agtcattgac
      601 cagaccctgc tgccccatcg ctttgcgata cttcgcctgg gtacgctggc agaggtggca
      661 cacgccatca aaaacatgaa tgtacgtggc gcgcctctca tcggtgcggc ggcatcttgc
      721 ggcatcgcgc tggccatgcg cgaagagagt gatgatgcga ccctggccgc tgcggtcgat
      781 ctactgtgcg ccacccggcc gacggcggtc aacctgcatt gggcggtcga gcgcatgctg
      841 cgtgcgctgc ggccgctgcc gccggcacgg cgagccgagg tagcaataac ggaagcggcc
      901 gccatctgcg aggaagatgt catcctcaac catggcatcg gccagcacgg cttgaagttg
      961 ctgcagcaac actacgcaga acggcaacgt ccgctcaacc tgctcaccca ttgcaacgcc
     1021 ggctggctgg ccaccgtgga ctggggcacc gcgctggcac cggtgtacgc cgctttcgat
     1081 gccggcattc cgctgcacgt gtgggtggac gaaacccgtc cgcgcaacca gggcgccgcg
     1141 cttaccgcct gggagttggg caagcacggc gtaccgcata cggtcatcac cgacaacgcc
     1201 ggcggccacc tgatgcagca tggcaaggtg gatgccgtca tcgtcggcgc tgatcgcgtc
     1261 accagccgcg gcgacgtgtg caacaagatc ggcacctatc tgaaggcctt ggccgcgcat
     1321 gacaacggca tcccgttcta cgctgctgtg ccgtcaccct ccatagactg gcgcatggcc
     1381 gacggcctga gcgaaattcc catcgaggtg cgcagcgcca aagaggtggc ctggatacag
     1441 gggcggctat ccaacgggga aattgctcag gtcaacttgc tgccggacgg aagtaccgca
     1501 gccaacccgg cattcgacgt caccccgtcg cggctggtga ccggcatcat caccgagcat
     1561 ggcgtgatcc atcctgacca gcttaggagt ctgtttccat gacgcacctg cgcgaacagt
     1621 tgctcgccgt cacccgcaaa ctatccgaac tcggtctcaa tcgcgggact gccggcaacg
     1681 ccagcgtgcg cgatggtact ggcttcctgg tcacgcccag cgggatgtcc gtggaggcca
     1741 tgacgcccca cgacatggtg tgggtggatt tcgacggcaa cgcccaaggc gagcgcaaac
     1801 cctccagcga atggcgcttc caccgcgaca tcctgcatgc acgcccggaa gtcggcgcgg
     1861 tgatccacac ccatgccatg ttcgcgacca cgctggcctg tctgcgccgc gacgttccac
     1921 ctttccacta catgatcgcc gtggctggcg gcgatagcct gcgctgcgcg ccttatgccc
     1981 tgttcggcac ccaggcgctg tcggatgctg cgctgaccgc actgcaagac cgcaaagcct
     2041 gcctgctggc caaccatggc atgatcgcag taggccgtga cctggaggcc gccctggcca
     2101 tgacacagga agtcgaaacc ctgtgcgaac agtattggcg caccttgcag gtcggcgggc
     2161 cgacgctgct gacgccgcag gaaatggatg aggcgctgga acagttcaag gactacggac
     2221 gctggcggca ggaatcgcaa taagccacag ctggccgcct tctaccagtt ccgcatcctg
     2281 ctgaggagta tgcgcctgcg tcacaccgaa aaagtagcgc cgatccggtt tggcccaagg
     2341 ggcgcttttc agcaatccgg tcacctcgat gatgccggaa tgttcctcgt cctcggtcat
     2401 gaattccgca ccgccgttag tgaataaacg ggactcggac tcgaacaact tctgcgccgt
     2461 atgcggccgg gccgggtcgt atagccagat cgcggccagc ctgtcgctct cgcccggatc
     2521 ctcctcgatc agcacgcggc cgtcgccgtc caccgcaagg ttatcgaaca tctgcgcgcc
     2581 aacctcgccc gcctccagca cctcgtcgac atggccaccc tgttctggat gctgcgaatc
     2641 atcgaaagcc agacggaaca gctgactttt cccgtcaatc ctgtccgtgg tggcaaacca
     2701 aaacacgttg cggtcgcgct tgtcccacgc accatcctcc ggccgcagca agggggtggc
     2761 gcccaattgg cgggcacggg cgcgcagcac cttgccgtcc agcccgacca cctcgcccag
     2821 cggcagcagg gagaaacgcc ggtcctcgaa acgcacggca taaagcgcgc cccctaccag
     2881 tccggccttt tctaccgggt tgccagttct gcgcttttcg ccgacataga cgtagacatg
     2941 gccgccgggg taatcatcca tgccgaccac taccgtgcga tggccggatt gcggcagagc
     3001 cagcaggttc tcccaagcca cccgccccag atgcggcagt tcgtaactga ccccggcctg
     3061 cgcaccgccg accacgtgag cgaaggcgcg cccgccagcg tctttttctt cctcgccgtt
     3121 gagcagcaga cggccatcga agccctcacc ttcagaaccg gtaaatgccg gatcggccgc
     3181 gaggtcggcc gagcagaatc gcccgaaagc gactgccgga accacgttgt gggaagcgga
     3241 aacagcatcc cacaactgca cctgccgtac gaggtcctcg cccctcaaca cagccagatc
     3301 agccaaccgg atagtccagc gcgaaacgaa agcgcccttg ccaccatgtg ccctgggcac
     3361 ccccttgtcg gcagggattt catggttcat cagcaccgtc agggtgccgt cgccgttatc
     3421 cagcgcaccc agcccgtcag gcaccccggc cagccggtaa ccgttgccgg ctgcctcccc
     3481 aaccgtgagc aacggcttta cttcggcgtc ccctatcagg ggatgcaggt aaggaacgga
     3541 cggcgccgcc tgcagggaca gggaacacat cagatagcac agcaatatca ggaaacgcat
     3601 gcctaagctc cagcaacaat gactcttgaa agcagggata aagtaccacc ttgggccgcg
     3661 atctcaaaaa aaacagcccg cagagcgggc tggagtgagg agacgcagag aaatgattct
     3721 agaaatcgaa ctgggcgcgg aagttcatgg cgttttcggt atcatcaaca gaatcgcagg
     3781 ccgccttgtt cgcgttggtc tgcgctgtcg ttgtgccatt tgcacaatcc agcttggtat
     3841 ggatgtagtc cagcaggaag cgggtgttcg gattgatgat ccatttcacc cctgccgtcc
     3901 aggcgtgcgc ctcgttgaat ttggcagtct gcgcagcagg cgtcggacct acagttgcag
     3961 ccagatacgg tgtctcgaag tcgctggcat ccagattggt gtagcgcagg ccgagttccc
     4021 aggcgcccca acccttgttg ccgccttcca tgcgcgggtt gaagtcgtta aacggtttga
     4081 tgcggtcgaa tttgccgttc ttgtaggaat cggcatactt ctcgcccgtc accaaccagg
     4141 tcagcgcggc gttccaggtc ttgatggtct tgctgtcatc cgtggcagtc ccggagacga
     4201 tcagcccctt gtcgttctgg tagtcggcat gtgcatactc ggactgcagc ttgaactggt
     4261 tgtaggcgaa tgcaccttcc aggccggtgc gcgtcaggtc gaagccgtcg cgcaggttgg
     4321 cggacttgaa cacgtcgagc ccgcggcctt cactgcgcaa ggtgacctgg gtactgtttg
     4381 cggttccgct ggtattgttg ttgccccagg aatcggaata ggccacgcca aggtgcgcca
     4441 ccatgtccgg gttgcccatc agttcggcga tgttggcagt cacgcggccg gtccaggcct
     4501 tgccatcatt ttgaatggag ttttcgttga agttctgcgc ggagctgttg atgttgctgc
     4561 caagcccgtt gaaataaccc accgcatagg tcgtaccttt cacagggata ccctggagca
     4621 tggcgccgcg gtcgatgccg ggggcaagct gtgccgcata gctgcgctcg gtaaagttga
     4681 tgaagcggga actggtgagt tcttccaggc tcatcggctg cttgaactgg cccatctgca
     4741 actgcgcata gtctaccgga cgcaggttca ggtagtactc atacatggcg ctgctgttct
     4801 gcgaagacgt gttggcgaag ttcgcgatcg ccgcgaagtc gatccagtca taaaaggtgc
     4861 ccttgacgcc cagataggcg cggcggatgt cccaagtgtc agcctggttg ttgacctgct
     4921 gtggatggaa ggtacgatag tcggcctgca cacggccgtt gatggacatc ttgaaggcct
     4981 tgtcgccgga ctcgaacacg atgccgtcct tgaacgaggc cttgatctcg ctcttcttct
     5041 tagcctcggc agcctctttc tcgccttggc ggcccttcat cagcagcgcg ccttcctctt
     5101 cggtcagaac gcctttcgcc atcagcgcat tgatgatgtc gtcgttgctg tcggccatcg
     5161 ccggcagggc gcccagcagc atggcgccag aaaccgaggc cacgacgccg cgcagtttga
     5221 tgcttttcat agaattgctc ccagttgaaa ttgaacaaaa acatgattgg ccaatcaggt
     5281 tttacaacgg gagcgattct gggttccgaa tgttacggat ttatgacctg aaacaaattt
     5341 gagatgtcat gttgcgcaaa tgcgacagtc cttgctgcgc gcgaccaggt atcctctttc
     5401 tttgggtatc ctctttctct ggcatgaaga tcattgatga tggggcgaag agccgtcccc
     5461 aatggcacag gctctccact ctccccatgc atttatggct cgatcgcgct tgcttggccg
     5521 gcgatacgca agccaccggc cttggaacta tccggcgtga atgcagctac gtcgcccgac
     5581 aactccccac cttcttcgat caggattttg ccatagcgta tttttccgct caccttgccg
     5641 gtcgagtgaa tgatcaactg gctgcgggcc gtcaattcgc catcgaactt gccgtggatc
     5701 tcggcaacat cgatgccgac cttgccggaa aaagcaccct cggccgcaat ctggatgacg
     5761 cggctgtcca tggtggcctc cacgcgccct tccaccacca acgtgtcgca attcagaatt
     5821 tctgccccct tgagtttgac ctccggcccg acaatcagac ggcttccgga aggctgatcg
     5881 gctttcggag cctcggccgg cttggcttcc gtggagaccg gggcggtagt cacggtggga
     5941 gtggattcag ccgggtagcc aggcaacgtg gatgtcgaaa ccggcctcgc ggaacccagg
     6001 ggcgcggggg aaatgggttt ggcaggaatg gcgccactgg gttcagtgtt gcgcttgaag
     6061 atattttgct tggatagcat ggggttacct cgaagtaatg atgacgaaaa gggtgttgat
     6121 atacacacca cggcgtctac ctcagcaact cacatgccag atactgctaa tccttatttt
     6181 tcatggtgtt acaaaattat tgcgatatcg ggacaacaga acttccatac aaagcgtcgc
     6241 cggatcacga cagattttgg tgcggaacgc ctactccatt gattcaatat gattatttat
     6301 gtcgctgttg cccaacgggc attcagtcca cgaccttgta tccggcctgc tctaccgtca
     6361 ttttcaattg ttccggctgc acccgcgcag gatcataagc aatcacggcg gtcgcctcga
     6421 tgtgtgaaac ctcggcttcc accacgccgg gctgcgccag caaggcttgg gcgatggcgc
     6481 tggcacaacc accacaggcc attccggaga cgtgcagggt gatcttttcc acgctaggcc
     6541 tcaggccgca tatgcggaaa caggatcaca tcgcgaatgc tggggctgtc ggtcagcagc
     6601 atgactaaac gatcaatccc gataccgcag ccgccggtgg gcggcatgcc gtattccagg
     6661 gcgcggatgt agtcggcgtc gtagtacatg gcttcgtcgt cgcccgcctc cttggcctcc
     6721 acctgctggc ggaagcgcgc agcctggtct tccgggtcgt tgagctctga aaatccattg
     6781 gcaatttcac gcccggtgat gaacagctcg aagcgctcgg tgatcccggg gcgctgatcg
     6841 gaagcgcgcg ccagcggcga cacttcgacc gggtagtcga tgatgtaggt cggctgccac
     6901 aactgcgctt cggccgtttc ctcgaacagc gccagttgca acgcgcccag ccccaagtgc
     6961 ggcagggcgt cgatgccgaa cctcttcagt tcggttcgca gaaaggcgct gtccgccagt
     7021 tgcgcatcgg tatattgcgg cgcgtatttc cggacggcgc cgacgatggt caggcgttcg
     7081 aacggcatgg acaaatccac ttcgcgacct tgatagatga cggtcgccgt accttgcgct
     7141 gcaatcgccg ccgcgcggat gcactgctcg gtgaagtcca tcagccactg gtaatcggtg
     7201 taggcggcgt agaactccat catggtgaat tccgggttgt ggcgtaccga aagaccttcg
     7261 ttgcggaagt tgcggttgat ttcgaacacg cgctcgaaac cgcccacgat caagcgcttg
     7321 aggaatagtt ccggcgcgat gcgcaggaac atctgcatgt ccagcgcgtt gtggtgcgtc
     7381 ttgaacggct tggctgccgc accgccggga atcgggtgca gcatcggcgt ttccacttcc
     7441 agaaactggt ggtccagcat gaagtggcgg atcgccgcca cgatcttgct gcgcgtaaca
     7501 aaagtcttgc gggtatcctc ggaaacaatc aaatccacgt agcgctggcg gtacttggtt
     7561 tcctggtcgc tcaagccgtg gaacttctcc ggcagcggac gaatcgactt ggtgagcagc
     7621 cttaaagcat ccaccttgac cgacagctcg ccggtcttgg tcttgaacag cacgccctcg
     7681 gcggcgacga tgtcgcccag atcccatttc ttgaaggcat cgtagacttc ctcgcttacg
     7741 ttatccctgg caataaacaa ctggatgcga ctgctcgggt cctggatggt ggcgaaactg
     7801 gccttaccca tcacccgctt cagcatcatg cggccggata cgcttacccg gatgccatgc
     7861 ggctccagtt cctcgcccgg tttctcgccg tgcgcggcat gcaagtccgc cgccagatgg
     7921 ctgcgccggt aatcgttggg aaaagcgacg ccctgctcgc gcatggcttt cagcttggcg
     7981 cgacgctcgg cgatgacgtg gctttcgtcc tggggaattt gatcttctgt cataaaaata
     8041 accgggtaat tgataagtta acttccaaaa taaaccacct gcattcccat cgcttgagca
     8101 acgccacgca tcactgcatc tgcagtcgcc agctctttaa ctccgatagc atgcgctgcg
     8161 gcaagatggg tggcatccag tgtacgcaat gcaaattggg gataatcctc gatcaagcgc
     8221 atcgcctcga caaaataggc atcggtcatg gggtgaatca ccaaaacgcc atcctgcaca
     8281 tccaaccgcg ccttatccat caccccttgc tcggcggcgc ttgaaatctc gccattgcgt
     8341 cgcttgcgcg ccacggtgca tcgcaactcc agcagcacca ggcggctcac tgctaccggc
     8401 gcgttttcca caaaatacaa atccgccgcg tggctatttc tttcgggaac gtaccgcttg
     8461 accagcgcgc tggtgtcaaa atagatcaaa gttagccccg cgcatcgcga tcttcacgga
     8521 tcaacacttc gctgggcact tcctgaaaag gcatggtgtc acgaaacgcc gccatcgaac
     8581 gcgcttttgg ccgcgtcgct accgccgcct cttcaaacgg caacacccgc gcaaacggct
     8641 tgccgtggtg cgtcagcacc acctcgccct ccgccgccag cgtctccacc agcacatcat
     8701 aactggcgcg aatctggcga acgcctaatg ttttcatggt tggctccttg ggtaatcggt
     8761 aatttgtgac acatcattat aaaatgtgtc acaaatcggg tcaacgcgta tttgcctcat
     8821 cccggtcaaa gcggaaatcg tcgggcaagc gtccgcgccg gttgttgagg cttgattcac
     8881 ccaaaatcgc cgcctcgatt tcggcggcgg cgcgtgtttt gaggctgccg gcctcggcgc
     8941 gcttggcggc ggtctgctgg cgggcggcgg tgatggcgtt gaccagggtt tgttgagttt
     9001 cgagcggggg cagcggcagg cgtaaaccac gtaattcttc gctgttgatg ttgttctgca
     9061 taatctgccg acttacagcg ttgacctgag atcgtcccaa agctgagttc agaaaaaagg
     9121 ccgcgaaatc cggatttagt ttggaggcat taacacacag tcgaatcaga taagaggcaa
     9181 acacatattc accaacctca tggaataccg cgcaagttcc taccaaatca cggctaccgt
     9241 ttgttcgaat gaccagaata tccccatcct tcagcagcaa tccttgagca actttggggg
     9301 gtaggttgat gtgctttaga tcggaaacat ccagcaaccc agccttgacg ttgttcatgc
     9361 gcagcaccgg cagcccattt ccatcttggt tggctttttc gctggttcca tattgcacaa
     9421 ggtcgatcgc ctcgccgagc gcaacggtag gaaattttcc aacatcaggg ctggtgccgg
     9481 aaatccgttg ctgattccac tccatccccc aacgttcaca atcgctccag cgcacggcga
     9541 aggccttgcg gcgtttggcc tcatcggggg cggccagtcc cagcccgtgc aggaaatccc
     9601 gcgcggcgtc ctgctccagc cggtcggcct ccagcagcgc ggc
//
LOCUS       DAOMFA010000058         9205 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_837_length_9205_cov_5.129508, whole genome shotgun sequence.
ACCESSION   DAOMFA010000058 DAOMFA010000000
VERSION     DAOMFA010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 9205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9205
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_837_length_9205_cov_5.129508"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1191)
                     /gene="purB"
                     /locus_tag="PKW44_04315"
     CDS             complement(<1..1191)
                     /gene="purB"
                     /locus_tag="PKW44_04315"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018077792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HQR50843.1"
                     /translation="MNLTALNALSPLDGRYQSKLDALRPYFSEYALIKHRAQVEVAWL
                     KALAAASQLGEIAPFSDATLKELNDAISSFGETEAGEVKAIEARTNHDVKALEYWLKE
                     RFYANPEIRKASEFIHFACTSEDINNLSHALMLKGARDDVLLPMLDQIIARLTAQAHD
                     LADAAMLAHTHGQPASPTTLGKELANVVYRLRRQRKQLAGTEILGKINGAVGNFNAHL
                     SAYPDFDWEAFAQQFVEGLGLSYNPYTIQIEPHDYMAELYDAINRINTILIDLDRDIW
                     GYISMGYFKQKVKEGEVGSSTMPHKVNPIDFENSEGNLGLANAVLGHLSEKLPISRWQ
                     RDLTDSTVLRNMGVAFGYSLLGYDSLLKGLNKLEANRAKLAKDLDASWEVLAEPIQTV
                     MRRYG"
     gene            complement(1375..2667)
                     /locus_tag="PKW44_04320"
     CDS             complement(1375..2667)
                     /locus_tag="PKW44_04320"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HQR50844.1"
                     /translation="MVTLLHRSEQAHDDSHVASRMLRRLLRGYPTALVVHLWDGRTLH
                     IGEGSPAFTLSLQHPGVLRDLVLYRDPVRLAEAYCSGEVDVTGNFDAAVNLRYYLERL
                     QLTFRERLLLAWRAFGLGSAERQLAREAAAASHLRHNSSESIAFHYDLSNAFYRLWLD
                     ERMVYSCAYFETPEQSLELAQRNKLDHICRKLRLRPGESLLDVGCGWGALVCWAARHY
                     GVRAHGITLSRNQFEHAQEEVRRQGLEAQVTIELRDYRHLPAEACYDKVVSVGMFEHV
                     GLKNLPGYFATVRRILKPDGLFLNHGITSDEPGWKRSVSSKFVNRHVFPDGELDTVSN
                     IQGVMEDSGFEIFDVEGLRPHYALTLRHWVDRLDAKREAAVRLVGERNYRVWRLYMTA
                     SAQQFENGATGIYQILAARKESRPPPLPLTRRDLYPGN"
     gene            complement(2883..3158)
                     /locus_tag="PKW44_04325"
     CDS             complement(2883..3158)
                     /locus_tag="PKW44_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR50845.1"
                     /translation="MIHHITDVPGNDTSRIVERPDGFYWIDYETEVEFGPFATAALAI
                     ADMTPALESSYEPGETLQEAEAELGICDWIDPETGAPAEDSVHRLED"
     gene            3278..3868
                     /locus_tag="PKW44_04330"
     CDS             3278..3868
                     /locus_tag="PKW44_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HQR50846.1"
                     /translation="MAMGDLELLLREVRACTVCAPYLPNPPKPVLRAGKSARLLIVGQ
                     APGRRVHESGIPWNDPSGDKLREWLQLSREQFYDESRVAIIPTGFCYPGKGAHGDLPP
                     RPECAPLWHPRLRAALPEVRLTLLVGSHAQAYYLGKRGKSTMSDTVCDYRTYLPQFFP
                     LPHPSPRNRLWLSRRPWFAAEVLPELQRRVNMALNQ"
     gene            3931..4170
                     /locus_tag="PKW44_04335"
     CDS             3931..4170
                     /locus_tag="PKW44_04335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR50847.1"
                     /translation="MLLLTLTLPLAALAENPTPSVEDNIRAMDTDRDGMVTVAEVRAY
                     LEARNGKGYKHELLVDMEAKANARSCASPFSQSFY"
     gene            4247..5755
                     /locus_tag="PKW44_04340"
     CDS             4247..5755
                     /locus_tag="PKW44_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HQR50848.1"
                     /translation="MTVIREQDFVDSIADALQYISYYHPADYIQALATAYQREQSPAA
                     KDAIAQILTNSRLCAEGKRPICQDTGIVVAFVKVGMAVRWDSRLSLEQMVNAGVRRAY
                     TNPDNPLRASMVTDPAGARNNTHDNTPAVVHVSLVPGDTVEVSIAAKGGGSENKAQLA
                     MLNPNDSIVDWILEVVPQMGAGWCPPGMLGIGIGGTAEKAVLLAKESLMAPIDMHELQ
                     GRGASNRLEQLRLEIYEKVNALGIGAQGLGGLTTVLDVKILDYPTHAASLPVAIIPNC
                     AATRHAHFVLDGSGPVQLEPPSLSDWPDVHWAPSRQARRVDLDTITHQQVASWQPGET
                     LLLNGRLLTGRDAAHKRIATLLAKGEPLPVDFRNRFIYYVGPVDPVRDEVVGPAGPTT
                     ATRMDGYTEMMLGQAGLLGMVGKAERGPEAIEAIRRHGSAYLMAVGGAAYLVSRAIKS
                     SRVVAFADLGMEAIYEFEVADMPVTVAVDSRGVSVHQTGPSQWVKIAATPGH"
     gene            5814..8381
                     /gene="acnB"
                     /locus_tag="PKW44_04345"
     CDS             5814..8381
                     /gene="acnB"
                     /locus_tag="PKW44_04345"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HQR50849.1"
                     /translation="MLNAYRTHVAERAALGLPPLPLNPEQTAALVELLKRPPAGEETL
                     LQDLLENRIPAGVDQAAYVKAAFLTDVASGTAKSPLVSPQKAVQLLGTMLGGYNVPAL
                     VALLDGPLAAEAVAALSHTTLMFDAFHDVQEKMLAGNAHARKLIESWANAEWFTSRPA
                     LPQQIRCVVFKVPGETNTDDLSPAQEAWSRPDIPQHARAMLVSKMPDGLATIETLKQK
                     GLPLAYVGDVVGTGSSRKSAINSVQWYMGEDIPHIPNKRTGGIVIGGKIAPIFFNTAE
                     DSGALPIQCDVAAMNTGDVIVIHPYAGKITDESGTTLSSFDLAPLTLPDEVRAGGRIP
                     LIIGRGLTAKAREALGLPSSDLFVLPAAPADTGKGYTLAQKMVGRACGVEGVRPGTYC
                     EPRITTVGSQDTTGAMTRDELKELACLGFSADLVMQSFCHTAAYPKPVDIKLQHTLPE
                     FMSSRGGVSLRPGDGVIHSWLNRMILPDTVGTGGDSHTRFPIGISFPAGSGLVAFAAA
                     MGAMPLDMPESVLVRFMGTMQPGITLRDLVNAIPYAAIRKGDLTVGKTGKKNVFNGRI
                     LEIEGLPDLKVEQAFEFADASAERSANGCTVHLNKAPVIEFLNSNIALMENMIENGYQ
                     DARTLRRRIEAMQAWLAKPELLQPDVDAEYAYVLDIDLNEIKEPLVACPNDPDDIKPL
                     SAVVGDRIDEVFIGSCMTNIGHYRAAAKVLDDAGLIPTRLWIAPPTRMDEAELREEGA
                     YAVFGRAGARTEVPGCSLCMGNQARVADKATVFSTSTRNFDNRMGKDARVYLGSAELA
                     AVCARLGKMPTPEEYLATVGDKLSRNAGEVYRYLNFDQMAEYQPKKVIPISEIGLGL"
     gene            8381..8803
                     /locus_tag="PKW44_04350"
     CDS             8381..8803
                     /locus_tag="PKW44_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019899217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR50850.1"
                     /translation="MAKRPTQQPAQLSEEELFAKPARASWKVMLPVWALLLGLVWLGL
                     HFGVDEHLVAGGAVLFGLLSSAFVWLVGVVGLVPFIGPLIVKVLAIPIIWLLNALGYL
                     VSFVAIRRGYSQDVLTYRGLTIAVIVGIVVGYILGKLF"
     gene            complement(8815..9093)
                     /locus_tag="PKW44_04355"
     CDS             complement(8815..9093)
                     /locus_tag="PKW44_04355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR50851.1"
                     /translation="MSEIEHFGSELVELDKQIVGLGTLCGLDTTQDAVINAILRDEIL
                     PEQLPQDQRGMFTLLKALLSMRYKVEKQCLDDFGPGKCREILDTTQLK"
BASE COUNT         1690 a         2855 c         2869 g         1791 t
ORIGIN      
        1 gccgtagcgg cgcattacgg tctggatcgg ctcggccaga acctcccacg aagcatccag
       61 gtccttcgcc agctttgccc ggttggcttc cagcttgttg aggcccttga gcagcgaatc
      121 gtagcccagc agcgaatagc cgaacgccac gcccatgttg cgcaggaccg tggagtcggt
      181 gaggtcgcgc tgccagcggg agatcggcaa tttttccgac aggtggccga gcacggcatt
      241 ggccaggccg aggttgcctt cggagttctc gaagtcgatc gggttgacct tgtgtggcat
      301 ggtggaggag ccgacttcgc cttccttgac cttctgcttg aagtaaccca tggagatgta
      361 gccccagatg tcacggtcga ggtcgatgag gatggtgttg atgcggttga tggcatcgta
      421 cagttcggcc atgtagtcat gcggctcgat ctggatggtg taggggttgt agctcaggcc
      481 cagaccttcg acgaactgct gcgcgaacgc ctcccagtca aaatcggggt aggcggacag
      541 gtgggcgttg aaattgccga ccgcgccgtt gatcttgccg aggatttcgg tcccggccag
      601 ttgtttgcgc tgacggcgca ggcggtacac gacgttggcg agctccttgc ccagcgtggt
      661 gggggatgcc ggctggccgt gggtatgcgc cagcatggcg gcgtcggcca gatcgtgcgc
      721 ctgcgcggtg agccgggcga tgatctggtc cagcatgggc agcagcacgt cgtcgcgcgc
      781 gcctttcagc atcagggcgt gcgacaggtt gttgatgtct tcggaagtgc aggcgaaatg
      841 gatgaattcg ctcgctttgc ggatttccgg attggcgtag aaacgctctt tcagccagta
      901 ttccagcgcc ttgacgtcgt ggttggtacg cgcctcgatg gccttgacct cacctgcctc
      961 ggtttcgccg aagctgctaa tggcatcgtt cagttccttc agcgtggcgt cgctgaaagg
     1021 tgcgatttcg cccagttgcg atgcagccgc cagtgccttc agccatgcca cttcgacctg
     1081 ggcgcgatgc ttgatgaggg cgtattcgct gaaataaggg cgcagcgcat ccagcttgga
     1141 ctggtagcgg ccgtcgagcg gagaaagggc attgagagcg gtgaggttca tggcgaaatg
     1201 atcgagtgtg cttgaagatt tgccatttta ccggtcttgg gcaacttttt cataaagatt
     1261 gccgttatgg tacccaagcc gtgcaggctt accttcccgc cctggtgtgg gagcgccgcc
     1321 ctcggcgcga tattctctat gatcgcggcg cggcgccgct cctgcagcca gactttaatt
     1381 gcctgggtaa agatcgcgcc gggtgagtgg cagcggcggc ggccggcttt ccttgcgtgc
     1441 ggccagaatc tgatagatgc cggtggcgcc gttttcgaac tgctgtgcgc tggctgtcat
     1501 atacagccgc cacacccggt aattacgttc accgaccagc cgcaccgcgg cctcgcgctt
     1561 ggcgtcgagg cgatccaccc aatggcgcag ggtgagcgcg taatgcggtc gcagcccttc
     1621 gacatcgaag atctcgaatc cgctgtcttc catgacgccc tggatgttgc tcacggtgtc
     1681 cagttcgccg tccgggaata cgtggcggtt gacgaacttg ctcgacacgc tccgtttcca
     1741 gcccggctcg tcgctggtga tgccatggtt gagaaacagg ccgtccggtt tcagtatgcg
     1801 gcgcacggtg gcaaagtagc ccggcagatt cttcagcccg acatgctcga acatgccgac
     1861 gctgactacc ttgtcgtagc aagcttcggc gggcaggtgg cgatagtcgc gcaactcgat
     1921 ggttacctgc gcctccagcc cttgccgccg cacttcttcc tgcgcatgct cgaactggtt
     1981 tcggctcagc gtaatgccgt gcgcacgtac gccgtagtgc cgtgccgccc agcacaccag
     2041 ggcgccccag ccacagccca catcgagcag ggattcgccc gggcgcaggc gcagcttacg
     2101 gcagatgtgg tcgagcttgt tgcgttgcgc aagttccagg ctctgctccg gcgtctcgaa
     2161 ataggcgcag gaatacacca tgcgttcatc cagccaaagc cggtaaaacg cgttggaaag
     2221 atcgtaatgg aaagcgatgc tctcgctgct gttgtggcgc aggtggctgg ccgctgccgc
     2281 ctcccgggcc aattgccgtt cagcggagcc cagcccgaat gcgcgccatg ccagcaatag
     2341 cctttcgcgg aacgtcagtt gcaggcgttc caggtaataa cgcaggttga ccgccgcgtc
     2401 gaaattcccc gtcacatcga cctcgccgct gcaatatgcc tccgccaggc gcaccgggtc
     2461 gcggtatagc accaagtcgc gcaacacccc gggatgttgc aagctcagcg taaacgccgg
     2521 ggacccttcg ccgatgtgca atgtcctgcc gtcccacaga tgcacgacca acgccgtggg
     2581 gtagcctctg agcaggcgcc gcagcatgcg gctggccaca tggctgtcgt catgcgcctg
     2641 ttccgaacga tgcaggagag tgaccatcgc tttattccac ggcgacgctg cgccgcttgg
     2701 aggattgcgt tgattccagg gagccatcca tgaagatggg tgtggcagga gtattccaga
     2761 tgctttcttt ttccatgact gtctccttgt ggatgcgcct tggatttcga acttccggaa
     2821 aaaggataac aaattgtaag ttaaagggaa acaggctttc tgcagatgca ggttcgcggc
     2881 tgtcagtctt ccaatcggtg tactgaatct tccgccggag cgccggtttc cgggtcgatc
     2941 cagtcgcaga tgccaagctc ggcttccgcc tcctgcaggg tttcccccgg ttcgtagctg
     3001 gattcaaggg ccggtgtcat atcggcaatg gccaaagccg ctgtcgcaaa agggccgaat
     3061 tcaacttcgg tttcgtaatc gatccagtag aagccatctg ggcgctcgac aatgcgcgaa
     3121 gtgtcatttc ccggtacgtc ggtgatgtgg tggatcatgt catttccccc gtctggcaag
     3181 cgatttcagt ataggctcga cttgcaagct tgtcattgat tgtcctgctc cggtgatgtc
     3241 actatcatgg cgctgtatcc atggaaaaag cgaggccatg gcaatgggcg acctggaact
     3301 actgctgcgc gaggtgcgtg cctgtaccgt gtgcgcccca tacctgccga atccgccgaa
     3361 acccgtgctg cgtgcaggga aaagtgcccg cctgctgatc gtcggacagg cgccggggcg
     3421 gcgggtgcat gaaagcggga tcccgtggaa cgatccttcg ggtgataaat tgcgcgagtg
     3481 gctgcaactg tcgcgcgagc agttttacga cgagagtcgc gtcgcgatta ttcccaccgg
     3541 tttctgctat ccgggcaaag gtgcgcacgg cgatctgccg ccgcgcccgg aatgtgcgcc
     3601 gctgtggcat ccccgtttgc gcgcggcatt gcccgaggtc cgtctgaccc tgctggttgg
     3661 cagccatgcc caggcgtatt accttggcaa gcggggaaaa tcgacgatgt cggacacggt
     3721 gtgcgactat cgcacctacc tgccgcaatt ctttccgctg ccacacccca gtccgcgcaa
     3781 ccggctgtgg ctgagccgcc gcccgtggtt tgcggcggaa gtgctgccgg aattgcagcg
     3841 gcgggtcaat atggcgctga accaatgagc ttgcgcgcgc tctattcggt atcggcttct
     3901 gcttatacaa tacacaccat gcctgctctc ttgctgctcc tgacactgac actgcccttg
     3961 gccgcgctgg cggagaaccc cacgccaagc gtcgaggaca acattcgtgc catggatacc
     4021 gaccgcgacg gcatggtcac cgtggccgag gtgcgtgcct acctggaagc caggaacggc
     4081 aagggctaca agcacgagtt gctggtcgat atggaggcca aggccaacgc cagaagctgc
     4141 gcctcgccct tcagccagtc cttttactaa gtccaacagg gcgaatgccg ttaccgatgc
     4201 caaccatgca tgccaggctg cggccgtatt cccttgggaa cccctcatga cggtgatacg
     4261 agagcaggat ttcgtcgaca gcatcgctga cgcgctgcaa tacatctcct attaccaccc
     4321 ggcggactac attcaagcgc tggcaacggc ctaccagcgc gagcaatcgc ccgccgccaa
     4381 agacgccatc gcgcagatcc tcaccaactc gcgcctgtgc gccgagggca agcgcccgat
     4441 ctgccaggac accggcatcg tggtggcctt cgtcaaggtc ggcatggcgg tgcgctggga
     4501 tagccgcctg agcctcgagc aaatggtcaa cgcgggggtg cgccgcgctt acaccaaccc
     4561 ggacaacccg ctgcgcgctt ccatggtcac tgacccggcc ggcgcgcgca acaacaccca
     4621 tgacaacacg cccgccgtgg tgcatgtatc gctggtgccg ggcgatacgg tggaggtcag
     4681 catcgccgcc aagggcggtg gttcggaaaa caaggcacag ctcgccatgc tcaaccccaa
     4741 cgacagcatc gtcgactgga tattggaagt ggtgccgcaa atgggcgccg gctggtgccc
     4801 gccgggaatg ctgggcatcg gtatcggcgg cacggcggaa aaggccgtgc tgctggccaa
     4861 ggaatcgctg atggcaccca tcgacatgca cgaactgcaa gggcgcggcg ccagtaatcg
     4921 cctggaacag ctacggctgg agatttatga aaaagtgaat gcgctgggta ttggcgcaca
     4981 ggggctgggc gggctgacca cggtgctgga cgtcaaaatc ctcgattacc ccacccacgc
     5041 cgcctcgctg ccggtcgcca tcatccccaa ctgcgccgcc acgcgccacg cgcatttcgt
     5101 gctggacggc agtggtccgg tgcagctgga accgcccagc ctgtccgatt ggccggacgt
     5161 gcactgggcg cccagccggc aggcacggcg tgttgatctc gacaccatca cgcaccaaca
     5221 agtggcgagc tggcagccgg gcgaaacgct gctgctgaac ggccgactgc tcacaggacg
     5281 cgatgccgcg cacaagcgca tcgccaccct gctggcaaaa ggcgagccgc tgccggtgga
     5341 ttttcgcaac cgcttcatct actacgtcgg cccggtggat ccggtgcgcg acgaagtggt
     5401 cggtccggct ggcccgacca ccgccacccg catggatggc tacaccgaaa tgatgctggg
     5461 acaggccggc ctgctcggca tggtgggcaa ggcggagcgc ggccccgagg cgattgaagc
     5521 catccgcagg cacggctctg cctacctgat ggcggtgggc ggcgcggcct acctggtctc
     5581 cagggcgatc aagtcctctc gcgtggtggc gttcgccgac ctcggcatgg aagccattta
     5641 cgaattcgag gtggcagaca tgccggtgac ggtcgcggtg gacagccgcg gcgtctccgt
     5701 gcaccagacc ggcccgtcgc aatgggtgaa aatagccgct acgcctgggc actgaaattg
     5761 cttctccaag gctataattc ctcatcccta tttacatcaa gcgataccac atcatgctca
     5821 atgcctaccg tacccatgtc gccgaacgcg cagcgctcgg cctgccgccg ctgccgctca
     5881 acccggagca gaccgccgca ctggtggaac tgctcaagcg cccgcccgcc ggtgaagaaa
     5941 ccctgctgca ggatttgctg gaaaaccgca tccccgccgg cgtcgaccag gctgcctatg
     6001 tcaaggccgc cttcctgacc gatgtcgcca gcggcacggc gaagtcgccg ctggtttcgc
     6061 cgcagaaggc ggtgcagctg cttggcacca tgctgggcgg ttacaacgtg ccggcactgg
     6121 tggcgctgct ggacggtccg ctggccgcgg aggcggttgc ggcgctgtcg cataccacgc
     6181 tgatgttcga cgccttccac gacgtgcagg aaaaaatgct ggccggcaac gcccatgcca
     6241 gaaagttgat cgaatcctgg gccaatgcgg agtggttcac ttctcgcccc gcgttgccgc
     6301 agcaaattag atgtgtggtg ttcaaggtgc cgggcgaaac caataccgac gacctgtcgc
     6361 cggcgcagga agcctggtcg cgccccgaca ttccacagca tgccagggcc atgctggttt
     6421 ccaagatgcc ggatggactg gccaccatcg aaacacttaa gcagaagggt ctgccgctgg
     6481 cctatgtcgg cgacgtggtc ggcaccggct cctcgcgcaa gtccgccatc aattcggtgc
     6541 agtggtacat gggcgaggac atcccgcata tccccaacaa gcgcaccggc ggtatcgtca
     6601 tcggcggcaa gatcgcgccc atcttcttca acaccgccga agattccggc gcgctgccga
     6661 tccagtgcga cgtggcggcc atgaacaccg gcgacgtcat cgtcatccat ccctatgccg
     6721 gcaagattac cgacgagtcg ggcacaaccc tcagcagttt cgacctcgcg ccgctgacgc
     6781 tgccggacga ggtgcgtgcc ggcggccgca tcccactgat tatcgggcgc ggtctgaccg
     6841 ccaaggcgcg cgaagcgctg ggcctgccgt cctcggattt gttcgtgctg ccagccgcgc
     6901 cggcggacac cggcaagggc tacaccctgg cgcagaagat ggtgggccgt gcctgtggtg
     6961 ttgaaggcgt gcgccccggc acctactgcg aacccaggat caccaccgtc ggctcacagg
     7021 acaccactgg tgccatgacg cgcgacgagc tgaaggaact ggcctgcctc ggcttctctg
     7081 ccgacctggt gatgcagagt ttctgccaca ccgcggccta cccgaagccg gtagacatca
     7141 agctgcagca caccctgccg gaattcatgt ccagccgcgg cggcgtcagt ctgcgtcccg
     7201 gcgacggcgt gatccactcc tggctcaacc gcatgatcct gccggatacc gtcggcaccg
     7261 gcggcgattc gcacacgcgc ttcccaatcg gcatctcgtt cccggccggc tccggacttg
     7321 tggcttttgc tgccgccatg ggcgccatgc cgctcgacat gccggaatcg gtgctggtgc
     7381 gcttcatggg caccatgcag cccggcatca cgttgcgcga cctggtcaat gccatccctt
     7441 acgcggcgat ccggaagggc gatctcacgg ttggaaagac gggcaagaaa aacgtgttca
     7501 acggtcgcat cctggaaatc gaagggctgc ccgacctcaa ggtggagcag gcgtttgagt
     7561 tcgccgatgc ttctgccgag cgctcggcga atggctgtac cgtgcacctc aacaaggcgc
     7621 cggtgatcga gttcctcaac tccaacatcg cgttgatgga aaacatgatc gaaaacggtt
     7681 accaggatgc ccgtaccctg cgccgccgca tcgaggccat gcaggcctgg ctggccaagc
     7741 cggaactgct gcaaccggat gtcgatgccg aatacgccta cgtgctggac atcgacttga
     7801 acgaaatcaa ggaaccgctg gtggcctgcc ccaatgatcc ggacgacatc aagccgctgt
     7861 ccgccgtggt gggcgacagg atcgatgaag tgttcatcgg ttcctgcatg accaatatcg
     7921 gccactaccg cgccgcggcg aaagtgctgg acgacgccgg gctgatcccg acccgcctgt
     7981 ggatcgcccc gccgacgcgc atggacgaag ccgagctgcg agaggagggc gcctatgccg
     8041 tattcggccg cgccggcgcc cgcaccgaag tgccgggctg tagtctgtgc atgggcaacc
     8101 aggcacgcgt ggcggacaag gctacggtgt tctccaccag cacccgcaac ttcgacaacc
     8161 gcatgggcaa ggacgcccgc gtctatctcg gctcggcgga attggccgcg gtgtgtgccc
     8221 gcttgggcaa gatgccgacg cctgaagaat acctggcgac ggtgggcgac aagctctcca
     8281 gaaacgccgg agaagtttac cgctacctca atttcgacca gatggcggaa taccaaccaa
     8341 agaaagtgat cccgatttcg gaaatcggcc tgggactttg atggcgaagc ggcccacaca
     8401 acaaccggcc caactgtctg aagaggaact attcgccaag cccgcgcggg ccagctggaa
     8461 agtcatgctg ccggtgtggg cgctgctgct cggcctggtc tggctggggc tgcatttcgg
     8521 cgtggacgag catctggtgg ccggcggagc ggtgctgttt ggcctgctgt ccagcgcctt
     8581 cgtctggctg gtgggcgtgg tcggactggt gccattcatc ggcccgctca tcgtcaaggt
     8641 gctggccatc cccatcatct ggctgctcaa cgcgctcggt tacctggtct ctttcgttgc
     8701 catccggcgc ggctattccc aggatgtgct gacctaccgc ggcctcacca ttgccgtcat
     8761 tgtcggcatc gtcgttggct acatccttgg caagctgttc tagtgactgg ctgattattt
     8821 gagctgggtg gtgtccagga tttcccgaca tttgccgggg ccgaaatcgt ccagacactg
     8881 cttttctacc ttatagcgca tcgacagcag cgccttgagc agcgtaaaca tcccgcgttg
     8941 atcctgcggg agttgttccg gcaggatctc gtcgcgcagg atggcgttga tgaccgcatc
     9001 ctgcgtggta tccagcccgc aaagtgtgcc aaggccgacg atttgtttgt cgagctcgac
     9061 gagctcgctg ccgaagtgtt ctatttcgct catatgggtg tccggaagtt caaaagccat
     9121 tcaggcaatg gcatattcaa gagtatgttt ggctgtcgta tccaggccca gtatacggga
     9181 gaggtagggc atggctgctt cgagt
//
LOCUS       DAOMFA010000059         9154 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_845_length_9154_cov_4.978459, whole genome shotgun sequence.
ACCESSION   DAOMFA010000059 DAOMFA010000000
VERSION     DAOMFA010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 9154)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9154)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9154
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_845_length_9154_cov_4.978459"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(233..309)
                     /locus_tag="PKW44_04360"
     tRNA            complement(233..309)
                     /locus_tag="PKW44_04360"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(273..275),aa:Pro,seq:ggg)
     gene            complement(404..838)
                     /locus_tag="PKW44_04365"
     CDS             complement(404..838)
                     /locus_tag="PKW44_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HQR50852.1"
                     /translation="MAEQAAKVTLPPIPAKRYFTIGEVSELCGVKPHVLRYWEQEFTQ
                     LKPVKRRGNRRYYQHHEVLLIRRIRELLYEQGFTISGARNRLGEADTRDHHAAEAQEI
                     SVVPNSLPPDPDDDADELPAFDFTQLRAQLRGVLDMLRSAAA"
     gene            complement(819..1121)
                     /locus_tag="PKW44_04370"
     CDS             complement(819..1121)
                     /locus_tag="PKW44_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HQR50853.1"
                     /translation="MTLTKADLADLLFEQVGLNKREAKDMVEAFFEEVRSSLEVGDSV
                     KLSGFGNFELRQKSERPGRNPKTGEEIPITARRVVTFHASQKLKFKVEETYGGAGS"
     gene            complement(1131..3488)
                     /gene="pheT"
                     /locus_tag="PKW44_04375"
     CDS             complement(1131..3488)
                     /gene="pheT"
                     /locus_tag="PKW44_04375"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HQR50854.1"
                     /translation="MKFSENWLRSFVNPPLDSAGLNHLLTMAGLEVEAQTAVAPAFSG
                     VVVAEILSAEKHPDADRLQVLKVDVGQTEPLQIVCGGANARAGLKSACALVGAKLPGF
                     DIRQAKVRGVESFGMMCSEKELGLATDSAGIIELPADASLGQDIRAYFDLDDQVFELK
                     LTPNRSDCLSVAGVARDVSALTGAGLCLLGIPEAAATSQKGKAVYVEAATACPRYCGR
                     VVNGVNANALTPGWMKRRLERSGLRSISALVDITNYVLLELGQPLHAFDLSKLTGDIV
                     VRFARSGEKIALLNEQLSELQTDMLVIADDSGPVALAGIMGGSVTAVSDTTTDVFLES
                     AFFAPAAIAGKARRLGLSTDSSFRFERGVDFAGTRQCLERATQLVLEICGGYAGEIIE
                     QVAKFPERAPVRLRVQRAAAVLGIALTEEKVAGLLNRLQFNFSVNGGVFEVTPPSYRF
                     DIAIEEDLIEEVVRLHGYEHLPALPPMSDGLAMLPSPEERLSLDSLRQTLAAADYFET
                     INYSFIDAAWERGLMGNADAISLRNPIASNMSVMRSGLWGGLLESLQYNLNRKQERVR
                     LFEVGACFARREQGYVETTRIAGLCYGDALPEQWGEAAREVDFYDVKADVEMLTRGRT
                     RFEAAQHQALHPGQSARVILDGQAIGWLGGLHPQWQQYYGLPRGAVVFELDAVPLLVR
                     KLPAYTEVAKFPLVRRDIALVVDETVSVQALLDVMLEARAEVVDTISLFDQYRGKGVG
                     DGKKSLAFLVVMQDTQRTLTDEEVDAAMARLVAAATKKYGAVLRS"
     gene            complement(3494..4477)
                     /gene="pheS"
                     /locus_tag="PKW44_04380"
     CDS             complement(3494..4477)
                     /gene="pheS"
                     /locus_tag="PKW44_04380"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HQR50855.1"
                     /translation="MQNLEEILKNAQGEFDACATLVDLEQAKARFLGKSGALTELLKG
                     LGKLSVEERPAAGAAINVVKQGVEQALQGRRNAILATEQAHKLGSETLDVTLPGRGRG
                     RGRLHPVTLTLQRIEELFRSIGFEVAEGPEIETDFYNFTALNIPEDHPARAMHDTFYV
                     DEQHVLRTHTSPVQVHFMQDRQPPLRIIAPGRVYRVDSDATHSPMFHQVEGLWVDEQV
                     SFANLKGVVQDFLQRFFERDDLQVRFRPSFFPFTEPSAEMDMSWNGGWLEIGGCGMVH
                     PNVFGHVGIDAEKYRGFAFGLGVERLAMLRYGVNDLRLFFESDLRFLKQFV"
     gene            complement(4546..4908)
                     /gene="rplT"
                     /locus_tag="PKW44_04385"
     CDS             complement(4546..4908)
                     /gene="rplT"
                     /locus_tag="PKW44_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HQR50856.1"
                     /translation="MPRVKRGVIARARHKKVLDAAKGYRGRRKNVYRIAKQAVMKAGQ
                     YAYRDRRQRKRQFRALWIARINAGARECGLTYSRFMNGLKKSAVEVDRKVLADLAVFD
                     KPAFAKFAEVAKSGLGAA"
     gene            complement(4921..5118)
                     /gene="rpmI"
                     /locus_tag="PKW44_04390"
     CDS             complement(4921..5118)
                     /gene="rpmI"
                     /locus_tag="PKW44_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008107656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HQR50857.1"
                     /translation="MPKMKTKSGAKKRFKFLGNGKVKRTHSHLRHILTKKTTKQKRKL
                     RGTAIISATDVKRVRAMMPTQ"
     gene            complement(5193..5720)
                     /gene="infC"
                     /locus_tag="PKW44_04395"
     CDS             complement(5193..5720)
                     /gene="infC"
                     /locus_tag="PKW44_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HQR50858.1"
                     /translation="MAQDKETRINGDITAPQIRLIGVDGEQLGIVSLSQALSLAEEAE
                     IDLVEIAPQAAPPVCRLMDYGKFKYHESKRQHEARLKQKQVQVKEVKFRPGTDEGDYQ
                     VKLRNLIRFLQEGDKAKITLRFRGREITHQELGLALLKRVEADLQEHAVVEQFPKMEG
                     RQMVMVLGPHKKQQK"
     gene            complement(5747..7663)
                     /gene="thrS"
                     /locus_tag="PKW44_04400"
     CDS             complement(5747..7663)
                     /gene="thrS"
                     /locus_tag="PKW44_04400"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018292422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HQR50859.1"
                     /translation="MPIIRLPDGSERQFDAPVSVAEIAASIGAGLARAALAGKVNGQL
                     VDTSHVIAEDADLAIVTERDADGLEIIRHSMAHLLAYAVKELFPEAQVTIGPVIDNGF
                     YYDFSYKRPFTPEDLEAIEKRMAELARRDIPVIRKVLPRDEAVTYFKSIGEHYKAEII
                     ASIPQGEDVSLYQEGDFTDLCRGPHVPSTGKLKVFKLMKVAGAYWRGDSKNEMLQRIY
                     GTAWAKKEDLDAYLHMLEEAEKRDHRKLGRQLDLFHMQDEAPGMVFWHPRGWTLWQEV
                     EQYMRAKFVEYGYQEVRTPLVMDRALWEKSGHWENYRDNMFTTASENRDYAVKPMNCP
                     GHVQIFNHGLHSYRELPLRLAEFGCCHRNEPSGALHGLMRVRGFTQDDAHIFCTEEQI
                     QCEVADFIVMLQRIYADFGFNDVLVKLSTRPEKRVGSDETWDKAEAALTAALQQNGLA
                     FDLQPGEGAFYGPKIEFTLKDSLGRLWQCGTIQLDFNLPVRLGAEYVAEDNSRKPPVM
                     LHRAIVGSMERFLGILIENHAGAMPLWLSPVQAVVMNITDAQAEYVEKVTAELKKNGF
                     RAQSDLRNEKINYKIREHSLQKMPYLLVAGEREMQSGQVAVRTRKGEDLGSMPLQAFI
                     ERLRGEVDSKGGAA"
     gene            7922..8812
                     /locus_tag="PKW44_04405"
     CDS             7922..8812
                     /locus_tag="PKW44_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HQR50860.1"
                     /translation="MQIVSRTNSPSEAKYSQMSTRFDSEQGILWTLLNPEAVPCFNLE
                     LLTELRQHHDSIESNGGQFMVAGQAYPIRYSVLASLTPGVFNLGGQLALFRQLIRNQD
                     RDSLLHYATKCIDVILQRVRHFESSLATITLIEGDALGGGFEAALTSDIIIAERSAQM
                     GFPEILFNLFPGMGAYSLVARKVGTKTAEKIILSGKMYSAEELHEMGIVDYVVEDGEG
                     VQAVYDYVKKQERRANGFLAVQKARHRFNPVTYQELMDITTVWADAALQLTEKDLKVM
                     DRFVRSQERLFVHPEMRHTA"
     gene            complement(8852..>9154)
                     /locus_tag="PKW44_04410"
     CDS             complement(8852..>9154)
                     /locus_tag="PKW44_04410"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HQR50861.1"
                     /translation="FLQTVVHVFISKTQKILDAMRTALANREYGALKELAHMVKGSSG
                     NVGAEALHEVCREILLCEQDKLELSGDELLRRANASFKSARILLLQYLGGANRISI"
BASE COUNT         1897 a         2768 c         2507 g         1982 t
ORIGIN      
        1 ccaacggcac ggaacaaatc acgcatactt tttacccggt catgtttccc ggctatgcca
       61 cgctgaagga attgcgcgag gatcagatca ggattgattt cacatatgaa acaatcgagt
      121 ttgaggatgg gaccagagct acgaaataag ggccattacc gaggatagcg cccacgaatt
      181 gagaaaggcc gcattaagcg gcctcttcat ttttacaacc tattgattta attggtcggg
      241 gcgagaggat ttgaacctcc gaccacctgc accccatgca ggtacgctac caggctgcgc
      301 tacgccccga accggcgcca tgagctacta ggctccacgg cgaaggcggc attatagcgg
      361 aagcagcgca gcctgccaaa ctgcaggaat cagcgcgaaa caatcatgca gcagcggagc
      421 gaagcatatc cagcacaccg cgcagttgcg cgcgcaactg ggtaaaatca aacgccggca
      481 gttcgtcggc gtcatcatcc ggatcgggtg gcagggaatt tggaacgacg gaaatttcct
      541 gcgcttcggc cgcatggtga tcgcgcgtat cggcctcgcc caagcggttg cgcgcgccgc
      601 tgatggtaaa tccctgctca tacagcaact cgcgaatacg acgaatcagc agcacttcat
      661 gatgctggta gtagcggcgg ttaccgcgcc gcttgaccgg cttgagctgg gtgaattcct
      721 gctcccaata gcgcagcaca tgcggcttga cgccgcacaa ctcgctcacc tcaccaatag
      781 tgaaatagcg tttggcgggg ataggcggca gggtgacctt agctgcctgc tccgccatag
      841 gtctcttcta ccttgaattt tagcttctgg ctggcgtgga aggtcaccac acgtcgggca
      901 gtaataggaa tttcctcgcc cgtcttgggg ttgcggcccg gacgctcgga cttctggcgc
      961 aactcgaaat tgccgaagcc agacagcttc acactgtcac caacctcaag gctgctgcgc
     1021 acttcttcaa aaaaagcctc gaccatgtct ttggcttcgc gtttattcaa ccccacctgc
     1081 tcgaacagca ggtcggccag atcagctttt gttagtgtca tgccaccctc ttaacttctt
     1141 aacacagcgc cgtatttttt tgttgccgcg gcgaccagcc gtgccattgc ggcatcgacc
     1201 tcttcatccg tcaacgttct ttgagtatct tgcataacta ccagaaacgc aagacttttc
     1261 ttgccatccc cgaccccttt tccgcggtat tggtcgaaca gggaaatggt atctaccacc
     1321 tctgcacgcg cctccagcat cacgtcaagc aaggcttgca cagagaccgt ttcatccacc
     1381 accagtgcga tgtctcggcg taccagtggg aattttgcca cctctgtgta tgcaggtagc
     1441 ttgcgtacca gcaaaggcac agcatccagt tcgaacacca ccgcgccgcg cggcaagccg
     1501 taatactgct gccattgcgg gtgcagtccg cccagccagc cgatggcctg gccatcgagt
     1561 atcacacgcg ccgactgtcc cggatgcagt gcctggtgct gcgccgcctc gaaacgggta
     1621 cggccacgtg tcagcatttc cacatctgcc tttacgtcat aaaagtccac ttcgcgcgcc
     1681 gcttcgcccc attgctcggg cagggcgtcg ccatagcaca acccggcgat gcgcgtcgtt
     1741 tccacgtaac cttgctcccg cctggcaaag caggcaccaa cttcgaacag gcgcacccgt
     1801 tcctgcttac ggttgaggtt gtattgcagg gattccagca ggccacccca caggccggaa
     1861 cgcatcacgc tcatgttgct agcgatcggg ttgcgcaagg agatggcatc cgcattgccc
     1921 atcagaccgc gttcccatgc agcatcaata aagctgtagt tgattgtttc aaagtagtct
     1981 gccgccgcca gtgtttgccg caggctatcc aagctcagcc gttcttcggg agacggcagc
     2041 atggccaagc catcgctcat cggcggcaag gctggaagat gttcgtagcc gtgcaagcgt
     2101 acgacctcct cgatcaggtc ttcctcgata gcgatgtcga agcgatagct aggaggcgtt
     2161 acttcgaata cgccgccgtt tacgctgaaa ttgaattgca ggcggtttag cagcccggca
     2221 actttttcct cggtcagggc aatgcccaat acagcggcgg ccctttgcac acgcagcctt
     2281 accggtgcac gctccggaaa tttagcgacc tgctcgatga tttcgccggc gtagccgccg
     2341 caaatctcca gcaccagttg cgtggcacgc tccagacatt gtctggttcc agcaaaatca
     2401 acaccgcgtt caaagcggaa ggaggaatca gtagaaagcc ctagtcggcg ggccttgcca
     2461 gcaatagcag caggcgcgaa gaaagcactt tccagaaaca cgtcagtggt ggtatcggaa
     2521 accgccgtga cactgccacc catgatgccg gccaaggcga cagggccact atcgtcggcg
     2581 atcaccagca tatcggtttg cagctcggac aattgctcgt tcagcagagc gatcttttcg
     2641 ccactgcggg cgaaacggac cacgatatca ccagtcagct tggagagatc aaaggcgtgc
     2701 aacggctgcc caagttccag cagaacgtaa ttggtaatat ccaccaacgc agaaatactg
     2761 cgcaagccgc tgcgctccag acggcgcttc atccagcccg gcgtaagcgc attggcgttg
     2821 acgccgttga ccacgcgacc gcagtagcgg ggacaggccg tggcagcttc cacgtacacg
     2881 gccttgcctt tttggctggt cgctgccgcc tcaggaatcc caagcaggca caacccggcg
     2941 cccgtcagcg ctgaaacgtc gcgcgccacg cctgccacgc tcaagcaatc gctgcggttg
     3001 ggggtaagct tgagttcgaa tacttggtcg tcaagatcga agtacgcacg aatgtcctgc
     3061 cccaacgatg catcggccgg cagttcgatg atgccggcgc tgtccgtggc caagcccagt
     3121 tccttttccg aacacatcat gccgaatgac tccacgccgc gcaccttggc ctgcctgatg
     3181 tcgaagccgg gcagcttggc gccgaccaag gcgcaggcgg atttcaaacc agcacgcgcg
     3241 ttagcaccac cacagacgat ctgcagcggt tcggtttggc ctacatccac tttcagcacc
     3301 tgcaagcggt cggcatcagg atgtttttca gcactgagga tttccgccac cacgacacca
     3361 ctgaaagcag gcgcgactgc agtctgcgct tcgacttcca gcccggccat tgtcaacaaa
     3421 tgattgagcc ccgcactatc aagtgggggg ttaacgaagc tgcgcagcca gttttcggag
     3481 aacttcatgg ttttcagaca aattgtttga ggaaacgcag gtcgctttcg aagaacaacc
     3541 tgaggtcatt aacgccgtag cgcaacatgg cgaggcgctc cacgcccaag ccaaaagcaa
     3601 agccgcggta tttctcggcg tcgataccga catggccgaa cacgttggga tgcaccatgc
     3661 cgcagccgcc gatttccagc cagccgccgt tccagctcat gtccatctcg gccgacggct
     3721 cggtgaacgg gaaaaacgac gggcggaaac gcacctgcaa atcgtcgcgt tcgaagaagc
     3781 gctgcaggaa gtcctgcacc acgcccttga ggttggcgaa gctgacctgc tcgtccaccc
     3841 acagcccttc cacctggtgg aacatgggtg aatgggtggc gtcggaatcg acgcggtaga
     3901 cccgccccgg cgcgataatg cgcagcggcg gctggcgatc ctgcatgaag tgtacctgca
     3961 ccggcgaggt gtgggtgcgc aacacgtgct gctcgtccac gtagaaagtg tcgtgcatag
     4021 cacgcgctgg gtggtcttcc gggatgttga gcgcggtgaa attgtagaaa tcggtctcga
     4081 tctccgggcc ttccgccacc tcgaagccga tggagcggaa cagctcctcg atacgctgca
     4141 gggtgagtgt gaccggatgc aatctccccc gccccctgcc gcgtccaggc agggtcacgt
     4201 ccagagtttc gctgcccagc ttgtgcgcct gctctgtcgc caggatggca ttgcgcctac
     4261 cctgcagcgc ctgttccacg ccctgcttga ccacgttgat ggcggcgccc gccgccgggc
     4321 gttcctctac ggacagtttg cccagcccct tgagcaactc agtcagtgcg ccactcttgc
     4381 ccaggaaacg cgccttggct tgctccaggt caaccagtgt tgcacaggca tcgaactcac
     4441 cttgggcgtt tttgagaatt tcttccagat tttgcatagt acggatcagt gtccctgccg
     4501 aaaaaaaagg aggccgctaa gcctcctttc ttcatttgct accgcttaag cagcgccgag
     4561 gccggacttc gccacttcag caaacttggc gaatgccggt ttgtcgaata cggccaggtc
     4621 agccagcacc ttgcggtcca cttccacggc tgacttcttc aggccgttca tgaaacggct
     4681 gtaggtcagg ccgcactcgc gggcgccggc gttgatccgg gcgatccaca gggcgcggaa
     4741 ctggcgcttg cgttgacgac ggtcgcggta ggcatattgc cccgccttca tcaccgcctg
     4801 tttggcgatg cggtatacat ttttgcgacg accacggtag cccttggctg cgtccagcac
     4861 cttcttgtga cgggcacggg cgatgacacc acgcttgact cttggcatgt ccttatctcc
     4921 ttattgggtc ggcatcatgg cgcgtacgcg cttgacgtcg gttgcggaaa tgatggccgt
     4981 gccgcgcagc ttgcgtttct gcttggtggt cttcttggtg aggatgtggc gcaggtgcga
     5041 atgggtacgc ttgaccttgc cgttccccag gaacttgaag cgctttttgg cgccgctctt
     5101 ggttttcatc ttgggcattt ctttactcct gtttttggat tactcaaaaa ggcgatcggg
     5161 catgccaggg ccgcgtcacc ttcggtttgg tgctacttct gctgcttttt atgggggccg
     5221 agcaccatca ccatctggcg gccttccatt ttcggaaact gttccaccac cgcatgctcc
     5281 tggagatcgg cctcgacgcg cttcagcaac gccaacccca actcctggtg ggtgatttca
     5341 cgaccacgaa aacgtagcgt aatcttggcc ttgtcccctt cctgcaggaa acggatcagg
     5401 ttgcgcagct tgacctgata gtccccttca tccgttcccg gacgaaactt cacttccttg
     5461 acctggactt gcttctgctt caggcgcgct tcgtgctgcc ttttgctctc gtggtatttg
     5521 aacttgccgt aatccatcaa gcggcaaacc gggggggctg cttgcggggc gatctcgacc
     5581 agatcgattt ctgcttcttc cgccagggac agcgcctggc tcaaactgac aatcccaagc
     5641 tgctcgccgt ccaccccaat caaacgaatc tgtggcgctg taatgtcacc gttgattcgt
     5701 gtttctttat cctgagctat cgcaaattct ccaagtcaat aaataatcag gccgcgccac
     5761 ccttgctatc gacctcgccg cgaaggcgtt caatgaacgc ctgcaacggc atggatccta
     5821 ggtcttcgcc tttccgggta cgcacggcca cttgtccgct ttgcatttca cgctcgcctg
     5881 ctaccagcaa gtaaggcatc ttctgcaagc tatgttcccg aattttatag tttattttct
     5941 catttctcaa gtctgattgt gcacggaagc cgtttttctt caattccgca gtcactttct
     6001 cgacgtattc cgcctgcgca tcggtgatat tcattaccac cgcctgcacc ggcgacagcc
     6061 acagcggcat ggcgccggca tggttctcaa tgaggatgcc gagaaagcgt tccatgctgc
     6121 cgacgatggc acggtgcagc atcaccggcg gcttgcgcga gttatcctca gccacgtatt
     6181 ccgcgcccag gcgcaccggc aagttgaagt cgagctggat ggtgccgcac tgccacaggc
     6241 ggccgagcga atccttcagc gtaaattcga tcttggggcc gtagaaagcg ccctcgcccg
     6301 gctgcaagtc gaacgccagg ccattctgtt gcaaggccgc ggtcaacgcg gcctcggctt
     6361 tgtcccaagt ttcatcggaa ccgacgcgct tttccgggcg ggtagagagt ttgaccagca
     6421 cgtcgttgaa accgaaatcc gcataaatcc gttgcagcat gacgatgaaa tccgccactt
     6481 cgcactggat ctgctcttcg gtgcagaaga tatgtgcatc gtcctgggtg aagccgcgca
     6541 cccgcatcag gccatgcagc gcgcccgaag gctcgttgcg gtgacagcag ccaaactcgg
     6601 ccaggcgcag cggcagttca cggtagctgt gcaggccgtg gttgaatatc tgtacgtggc
     6661 ccgggcagtt catcggcttc accgcatagt cgcgattttc ggaggccgta gtgaacatgt
     6721 tgtcgcggta gttttcccag tggccggact tttcccacag tgcgcgatcc atcaccagtg
     6781 gcgtacgtac ttcctggtag ccgtattcga cgaacttggc gcgcatgtat tgttcgactt
     6841 cctgccacag cgtccagccg cgcgggtgcc agaacaccat gccgggcgct tcgtcctgca
     6901 tgtggaacag gtcgagctgc ctgcccagct tgcggtggtc gcgtttttcc gcctcctcca
     6961 gcatgtgcag gtaggcgtcc aggtcttcct tcttcgccca ggccgtgcca tagatgcgct
     7021 gcagcatctc gttcttggaa tcgccgcgcc agtaggcgcc ggccaccttc atcagcttga
     7081 acaccttgag cttgccggtg gaaggcacgt gcgggccgcg gcagagatcg gtgaagtcgc
     7141 cctcctggta cagcgacaca tcctcgccct gcggtatgct ggcgatgatt tcggccttgt
     7201 agtgttcgcc gatggatttg aaataggtca ccgcctcatc gcgcggcagc accttgcgaa
     7261 tgacagggat gtcgcgccta gccagttccg ccatgcgctt ctcgatcgcc tccaggtctt
     7321 ccggggtaaa cgggcgtttg taggagaaat cgtagtagaa gccgttgtcg atcaccgggc
     7381 cgatggtcac ctgcgcttcc gggaacaatt ccttgaccgc ataagccagc aggtgcgcca
     7441 tggagtgacg gatgatttcc aggccgtcgg cgtcgcgctc ggtaacgatg gcgagatcgg
     7501 catcttcggc gatcacatgg gaggtgtcga ccaattggcc gttgaccttg ccggccaacg
     7561 ccgcgcgcgc caatccggcg ccgatgctgg cggcgatttc agccacgctg acgggagcat
     7621 cgaattgacg ctctgaacca tcgggtaaac gaatgatggg caatggatca gaatccgatt
     7681 cctaccggta gaaacaacaa aagccggaaa acggcttttg atcatcttta acgggtcgca
     7741 ttgtcgcaca aagcgccccc gcaggacaag gcggacctag ctgacaccca ttcctgcgac
     7801 gtcactatta attaagccaa aaaaaacatg cggaatgact cctactgcat tgattgggct
     7861 acaattcctt aagcctactg gctcagttaa acatcatcac ttgccaaatg aggggaaaaa
     7921 aatgcaaatc gtctctcgca ccaacagtcc gtctgaagcc aaatattccc agatgtctac
     7981 ccgcttcgat agcgaacagg ggatactctg gacactgctc aacccagagg cagtaccatg
     8041 cttcaatctg gaactcctga ccgaactccg gcagcatcat gacagcatcg aaagcaatgg
     8101 tggccagttc atggtggccg ggcaagcgta tcccatccgc tattccgttc tggcctcgct
     8161 gactccggga gtgttcaacc tcggcggcca gcttgccctg ttccggcaat taatcaggaa
     8221 tcaggatcgt gattcactat tgcactatgc caccaagtgc atcgatgtga tcctgcagcg
     8281 cgtgcgccac ttcgaatctt ctttggcaac catcacgctg atcgaaggcg atgccttggg
     8341 cggggggttt gaagccgcct tgaccagcga tatcattatt gccgagcgca gtgcgcagat
     8401 gggattcccg gaaatcctgt tcaatctttt tcccgggatg ggtgcttaca gcctcgtcgc
     8461 cagaaaagta ggcacgaaaa ccgccgaaaa aatcattctg agcgggaaga tgtacagcgc
     8521 ggaagaactc catgagatgg ggatcgtcga ttacgtggtc gaggatgggg aaggcgtaca
     8581 ggcggtctac gactatgtaa aaaagcagga gcgccgcgcc aatggcttcc ttgcagtgca
     8641 gaaagcccgc cacagattca atccggtgac ctatcaggag ctaatggaca ttaccacggt
     8701 ctgggccgac gcagcactgc aactcacgga aaaggatctc aaggtcatgg atcgtttcgt
     8761 gcgctcgcag gaaagactgt tcgtccatcc ggaaatgcgg cataccgcat aagcgcgtgc
     8821 gctgggccga tatgttgctt gtatcggccc cctaaattga aatacgattg gcgccgccca
     8881 agtactgcag cagcagtatt ctggccgact tgaagcttgc atttgcccgt cgcagcaact
     8941 catcgccaga gagttcgagc ttgtcttgtt cgcacagcag aatttcccgg catacctcat
     9001 gcaaggcttc cgcgccgacg ttcccgctac tccccttgac catgtgtgcc agttccttca
     9061 gcgcaccata ctctcggttt gccaaggcgg tccgcattgc atcgagtatt ttctgggttt
     9121 tcgagatgaa cacgtgcacc acagtctgca ggaa
//
LOCUS       DAOMFA010000060         9135 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_854_length_9135_cov_4.987665, whole genome shotgun sequence.
ACCESSION   DAOMFA010000060 DAOMFA010000000
VERSION     DAOMFA010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 9135)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9135)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9135
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_854_length_9135_cov_4.987665"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..198)
                     /locus_tag="PKW44_04415"
     CDS             complement(<1..198)
                     /locus_tag="PKW44_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein O N-terminal
                     domain-containing protein"
                     /protein_id="HQR50862.1"
                     /translation="MAIGGVARHDQQPVFVLHTYPFKETSLVVELFSRNFGRVAAVAK
                     GARRPRSAMRGMLQSFQPLLAA"
     gene            complement(198..1085)
                     /gene="era"
                     /locus_tag="PKW44_04420"
     CDS             complement(198..1085)
                     /gene="era"
                     /locus_tag="PKW44_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HQR50863.1"
                     /translation="MPEKAFHCGTVAIVGRPNVGKSTLLNHILGVKLSITSRKAQTTR
                     HRLLGIHTTDDTQFLFVDTPGFQLSHRSALNRVMNRTVQQVLAEVDVVLFVVESSRFG
                     QADRKVLELLPQNRPVLLVVNKADLLDDKAQLLPYIEQRSAEFQYTEIVPVSAKRSLN
                     LDDLLAAVRRHLPEQPAIYGEDELTDRNERFLAAELLREKVFRLLGEELPYSMTVEIE
                     KFEQEGALRRIHAAIIVDKEGQKPILIGKKGEKLKQISTEARQDMEKLFGGKVWLETW
                     VKVRGGWADDERALKSLGY"
     gene            complement(1078..1749)
                     /gene="rnc"
                     /locus_tag="PKW44_04425"
     CDS             complement(1078..1749)
                     /gene="rnc"
                     /locus_tag="PKW44_04425"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HQR50864.1"
                     /translation="MSLVELSRRLGHDFADQFLLQQAVTHRSHGQPNNERLEFLGDSV
                     LNCVVASLLYRRFSKLPEGDLSRLRAHLVKEPTLGGIALGLTLGDELRLGEGELKSAG
                     WRRPSVLADALEAIIGAVFLDGGFGAAEALVSRLYAPLLENLDPKSIGKDPKTLLQEY
                     LQGRRLALPEYVLLATEGEAHCQIFRMACRIPALNISAEGEGGSRRAAEQKAAEAAYH
                     EATHA"
     gene            complement(1753..2142)
                     /locus_tag="PKW44_04430"
     CDS             complement(1753..2142)
                     /locus_tag="PKW44_04430"
                     /inference="COORDINATES: protein motif:HMM:NF027463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4845 domain-containing protein"
                     /protein_id="HQR50865.1"
                     /translation="MRKQKGFTFWGLVFIVVLVVSAAIVAMKVIPRYTEYFTIKRALV
                     QIGKGNPAEMEDRDIRAAFERALDVNDIDIVTKNDIEIIRNANGQTELVVEYPAKVPL
                     VANVSLWLDFRASSDEKAVQSDSAANQ"
     gene            complement(2155..2901)
                     /gene="lepB"
                     /locus_tag="PKW44_04435"
     CDS             complement(2155..2901)
                     /gene="lepB"
                     /locus_tag="PKW44_04435"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HQR50866.1"
                     /translation="MSFALFMLLLLMLTGLLWLLDILVLRKRRPHGAPEPIAIEYAKS
                     FFPVILVVFLIRSFLVEPFKIPSGSMMPTLLTGDFILVNKYTYGLRVPVLNNVFFAVG
                     EPQRGDVMVFHFPKDPALDYIKRVVGLPGDRVAYRDKQLYINDKPVGLVFEDNYNYVA
                     PGLNQIAAKRYKEQLGEHRHDVLMVDEAPTMDGEVVVPQGYYFVMGDNRDNSNDSRFW
                     GFVPENNIVGKAFMIWWNFDNFGRIGDTIN"
     gene            complement(2961..4754)
                     /gene="lepA"
                     /locus_tag="PKW44_04440"
     CDS             complement(2961..4754)
                     /gene="lepA"
                     /locus_tag="PKW44_04440"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HQR50867.1"
                     /translation="MNNIRNFSIIAHIDHGKSTLADRIIQMCGGLSDREMEAQVLDSM
                     DLERERGITIKAQTAALSYKARDGKIYNLNLIDTPGHVDFSYEVSRSLAACEGALLVV
                     DASQGVEAQSVANCYTAIEQGVEVAAVLNKIDLPSADPERVIQEIEDIIGIEAIDAVR
                     CSAKTGEGVEEVLEAVVAKIPPPKGDPDAPLKALIIDSWFDNYVGVIMLVRVVDGSLK
                     PKDKIRLMATGDEYLCEQIGVFTPKSRQRESLSAGEVGFIIAGIKELLSAKVGDTVTL
                     AGRPAVEPLPGFKEIKPQVFAGLYPVESNQFEALRDALEKLRLNDSSLQFEPENSTAL
                     GFGFRCGFLGLLHMEIVQERLEREYDMDLITTAPTVVYELLMKNGEVVQIENPSRLPE
                     PSRVEEIREPIITVNLLMPQEYVGPVMTLCNGKRGVQRNMQYMGRQVMLTYEMPLNEV
                     VLDFFDKLKSVSRGYASMDYEFLEFRAADLVKLDIMVNGERVDALSLIVHRANSQYRG
                     RELCSKMRELIPRQMFDIAIQAAIGANIIARETVKAMRKNVLAKCYGGDITRKKKLLE
                     KQKEGKKRMKQVGNVEIPQEAFLAILRVEDK"
     gene            4970..5773
                     /gene="map"
                     /locus_tag="PKW44_04445"
     CDS             4970..5773
                     /gene="map"
                     /locus_tag="PKW44_04445"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HQR50868.1"
                     /translation="MAITLKDSNDIEKMRVAGRLAAEVLDYITPFVKPGVTTGELDKL
                     CHDYMVEVQGCIPAPLNYAPPGHQPYPKSICTSVNHQVCHGVPGEKILKNGDIVNLDI
                     TTIKDGYHGDTSRMFCVGTPSVQAKRLCDITHECMWLGIARVKSGATLGDVGHAIQQH
                     AEKNGYSVVREFCGHGIGKKFHEDPQVLHYGKPDTGLKLQAGMIFTIEPMINAGKRDI
                     RQLGDGWTIVTKDHSLSAQWEHTVLVTAEGYEVLTVSDGTPPAPTFLPR"
     gene            5770..8337
                     /locus_tag="PKW44_04450"
     CDS             5770..8337
                     /locus_tag="PKW44_04450"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HQR50869.1"
                     /translation="MTPLAPAIIATWRLQLQQRQALLRAEFERDADTARLLRYHCRLV
                     DQLLCDIWLETGLSGELCMVAVGGYGRGELFPHSDVDLLILLPDGHAEEFNSRLEPLI
                     GLFWDIGLAVGHSVRSLGECLDEAAADVTVRTNLLEARWLAGDRHAFQRMRKRLAEAM
                     NATEFLQAKQLEQEQRHMRFNDTAYNLEPNIKESPGGLRDLQNVLWISQGLGLGDNWG
                     ALARNGLITAPEARQIRRHELFLQMLRVRLHYLAGRREDRLLFDFQNELAAQLGLAGS
                     SRRRASEQLMQQYYRSAKFVRLMNEILLQNLQERIAEFPQKIIAINPRFQARNGLLEA
                     TSSSLLQREPRTILESFLLLEQHPELKGMSAPMLRTLWRVKGLVNREFRRDAGNKRLF
                     LEILRQPAGVTVALRRMNRYGILGRYIPAFGRISGQMQHDLFHVYTVDEHILNVLRNL
                     RRFATPKYGYEFPLCSQLITTFDKPELLYLGALFHDIAKGRGGDHSMLGTADAKRFCK
                     QHGLPRQDIELVAWLVEKHLLMSSTAQKCDLTDPSVIEGFARQMRDEYHLVALYLLTV
                     ADIRGTSPAVWNAWKAKLLENLFLATRRLLRGTSISVDAEVAARQQQAAITLSHYAIA
                     SDAYQELWNKLGRGYFLRHESQDIAWHTRVLLAHLNSTQPIVRSRLSPGGDGIQVLVY
                     SRDSDDLFARICGFFERINYTIVEARIHTTRHGHALDSFLVLDENEKGVRYRDLLSYI
                     EYELTQRIASPEMPQEPSYGRVSRQLKHFPMQPDISITPEANGQNHLLSITAGDRPGL
                     LSRISHIFLRHGVCLHTAKINTLGNRAEDTFLISGGQGNRLTDSVTQHLKKELSETL"
     gene            complement(8376..8465)
                     /locus_tag="PKW44_04455"
     tRNA            complement(8376..8465)
                     /locus_tag="PKW44_04455"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8429..8431),aa:Ser,seq:gga)
     gene            complement(8533..>9135)
                     /locus_tag="PKW44_04460"
     CDS             complement(8533..>9135)
                     /locus_tag="PKW44_04460"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HQR50870.1"
                     /translation="RLKNNESRIADSHEAVTILFADLVDFTHISSGMPPAALVDMLNQ
                     VFCRFDALTEKYGLEKIKTIGDAYMVVGGAPVPRLDHAEAVARLALEMQHVLAEVSAS
                     NGRGLKMRIGINTGPVIAGVIGSIKFSYDLWGDTVNMASRMEQYGLPNTIQISASTYE
                     LLKDKFVVKSRGIIAVKGKGDIETYILTGLAGDPGAPVRA"
BASE COUNT         1954 a         2809 c         2545 g         1827 t
ORIGIN      
        1 cgccgccagc agcggctgga acgattgcag catgccacgc atggcggagc gcggccgccg
       61 cgcacccttg gccaccgccg caacccggcc gaaattgcgg ctgaacagtt cgacgacgag
      121 actggtttcc ttgaacgggt aggtatgcag cacgaatacc ggctgctgat cgtggcgggc
      181 aactccccca atagccatca gtaccccagg cttttcagcg cccgttcgtc gtcggcccag
      241 ccgccgcgca ccttgaccca ggtttccagc cagaccttgc cgccgaagag cttttccatg
      301 tcctggcgtg cctctgtgga gatctgcttg agtttctcac ccttcttgcc gatcaggatg
      361 ggcttctggc cttccttgtc gacgatgatg gcggcatgga tgcggcgcag ggcaccttcc
      421 tgctcgaatt tctcaatctc taccgtcatc gaatacggca gttcctcgcc cagcagtcgg
      481 aacacttttt cgcgcagcag ttcggcggcc aggaagcgtt cattgcggtc ggtcagttcg
      541 tcttcgccgt agatggcggg ctgctccggc agatggcggc ggacagcggc aagcaggtca
      601 tcaagattga ggctgcgctt ggcgctgacc ggcacgattt cggtgtactg gaactcggca
      661 gagcgttgct cgatgtaggg tagcagctgt gccttgtcat ccagcagatc ggccttgttg
      721 accaccagca gcactgggcg gttttgcggc agcagttcca gcaccttgcg gtcggcctgg
      781 ccgaagcggc tggattccac gacgaacaac accacgtcga cctccgccag cacctgctgc
      841 acggtacgat tcatcacgcg gttgagcgcg ctcctgtggc tcaactggaa acccggcgta
      901 tcaacgaaca ggaactgcgt atcgtccgtg gtatggatcc ctaacaaacg gtggcgcgtg
      961 gtctgcgcct tgcgtgaagt aatgctgagc ttgacgccaa ggatgtgatt gagcagcgtg
     1021 gatttgccga cgttgggacg gccgacgatg gcgacggtgc cgcagtgaaa cgctttttca
     1081 ggcatgtgtg gcctcgtgat aagcagcttc ggccgctttt tgttcggccg cacgacggct
     1141 accgccctcg ccctcggcgc tgatattgag cgcaggaata cggcaggcca tacggaaaat
     1201 ctggcagtgc gcctcgcctt cggtggccag cagcacgtat tccggcagcg ccagcctgcg
     1261 accttgcagg tattcctgca gcaaggtctt gggatccttg ccaatcgatt tcggatcgag
     1321 attttccaac aagggcgcat acagccgact gaccagtgcc tcggcggcgc caaaaccacc
     1381 atcaaggaac actgcgccga taatcgcttc cagcgcatcc gccagcaccg aagggcggcg
     1441 ccagccggcg ctcttcagtt cgccctcgcc caggcgaagt tcgtcgccaa gggtaaggcc
     1501 caacgcaatt ccgcccaagg taggctcctt gaccagatgc gcacgcagcc ggctcaagtc
     1561 gccctccggc aatttggaga aacgcctgta gagcagactg gcgacaacgc aattgagcac
     1621 gctgtcgcca aggaattcca gccgctcgtt gttaggctgg ccatggctgc ggtgggtaac
     1681 tgcctgctgc agcaggaact gatcggcaaa gtcatgaccc agacgccggg acagttcaac
     1741 taaagacatg ggtcactgat tcgcagcact atcgctctgc actgcctttt catcggaact
     1801 ggccctgaaa tcgagccaca ggctgacatt ggcaaccaga ggtaccttgg ccgggtattc
     1861 gacgaccagc tcggtttgac cattggcatt cctgatgatc tctatgtcat tcttggtcac
     1921 gatatcgata tcgttgacat cgagtgcgcg ttcaaaggca gcgcggatat ctctgtcctc
     1981 catttccgcc gggttgcctt tcccgatctg gaccagcgcc cgcttgatcg taaaatactc
     2041 cgtgtagcgc gggataacct tcatggccac gatggcagcg gacacaacca gcacgactat
     2101 gaaaaccaag ccccagaaag taaagccttt ttgtttgcgc atcgttatcg tcctctagtt
     2161 gatggtatcg ccaattctgc cgaaattatc gaaattccac cagatcatga aggccttgcc
     2221 gacgatattg ttttccggta cgaaacccca gaaacggctg tcgttgctgt tatcccggtt
     2281 gtcgcccatg acgaaataat acccttgcgg taccactacc tcgccatcca tggtaggtgc
     2341 ctcatcgacc atcagaacat cgtgccggtg ttcgcccaat tgctccttgt agcgcttggc
     2401 ggcgatctgg ttgagcccgg gagcaacgta gttgtagttg tcttcaaaca ccagccctac
     2461 cggcttatcg ttgatataca actgcttgtc gcggtaagcg acccggtcgc caggcaggcc
     2521 gactacgcgt ttgatgtaat ccagcgcggg atccttggga aaatggaata ccatcacgtc
     2581 gccgcgttgc ggctcaccca ccgcaaaaaa tacgttgttc aggacaggta cgcgcaagcc
     2641 gtaagtgtat ttgttgacca ggataaagtc gcccgtgagc agggtcggca tcatcgaacc
     2701 ggaaggaatc ttgaacggct ccaccaggaa cgagcgtatc aggaacacca ccagaatcac
     2761 cggaaaaaaa ctcttggcat actcgattgc gatcggctcc ggggccccat ggggacggcg
     2821 cttgcgcaag accaggatgt caagcagcca cagcaaccct gtcaacatca gcagcagcag
     2881 catgaacagg gcaaagctga tgatgctctg gtacaccagc accagcccca gggccaccaa
     2941 tccaaaaaat acgtacgcca tcatttatcc tccacgcgca ggatggcgag gaaagcctcc
     3001 tgcggaatct ccacgttgcc cacctgcttc atgcgcttct tgccttcctt ctgtttttcc
     3061 agcagtttct tcttgcgcgt gatgtcgccg ccgtaacact tggccagcac gttcttgcgc
     3121 atggctttca ccgtttcacg cgcgatgatg ttggcgccaa tggcggcctg aatggcaata
     3181 tcgaacatct ggcgcgggat cagttcacgc atcttgctgc acaactcgcg gccgcggtac
     3241 tggctattgg cgcggtgcac gatcagcgac agcgcatcca cgcgctcgcc gttcaccata
     3301 atgtccagct tgaccagatc ggcggcacga aattccagga attcgtaatc catcgatgcg
     3361 tagccgcgcg agaccgactt gagcttgtcg aagaaatcca gcaccacttc gttcaacggc
     3421 atttcgtagg tcagcatcac ttgccgcccc atatactgca tgttgcgctg cacgccgcgc
     3481 ttgccgttgc atagcgtcat caccgggccg acatactcct gcggcatcag gaggttgacg
     3541 gtgatgatag gctcgcggat ttcctccacg cgagaaggct cgggcaggcg cgagggattc
     3601 tcgatctgca cgacctcgcc atttttcatc agcagttcgt acaccaccgt cggcgcagtg
     3661 gtgatcaaat ccatgtcgta ttcgcgctcc agccgctcct gcacaatttc catgtggagc
     3721 aggccaagga agccacaacg gaaaccgaac cccagggcgg tcgaattttc cggctcgaac
     3781 tgcaaggacg agtcgttcag gcgcaatttt tccagcgcgt cgcgcagcgc ctcgaactgg
     3841 ttggattcga ccggatagag accggcgaac acctgcggct tgatctcctt aaaaccgggc
     3901 aatggttcca cagccggccg gccggccagg gtcacggtgt cgcctacctt ggcactcagc
     3961 aattccttga taccggcgat gatgaatccc acttcgccag ccgagagact ctcgcgctgg
     4021 cgcgacttgg gtgtgaacac gccgatctgc tcgcacaggt actcgtcgcc ggtcgccatc
     4081 agcctgatct tgtccttggg cttgaggctg ccgtccacca cgcgcaccag catgatgacg
     4141 ccgacgtaat tatcgaacca ggaatcgatg atgagcgcct tcagcggcgc atcggggtcg
     4201 cccttgggcg gcgggatttt cgccaccacc gcctccagta cctcttccac gccctcgccg
     4261 gtcttggccg aacagcgcac cgcatcgata gcctcgatac cgatgatgtc ctcgatttcc
     4321 tgaatcacgc gctccgggtc ggcagacggc aggtcaatct tgttcagcac cgccgccact
     4381 tccacgccct gctcgatggc ggtgtagcag ttggcaacgc tctgcgcttc caccccctgc
     4441 gaggcatcca ccaccagcag cgcgccttcg caggcggcca gcgagcgcga gacctcgtag
     4501 gaaaagtcca catgccccgg ggtatcgatc aggttgagat tgtagatttt gccatcgcgc
     4561 gccttgtagc tcagcgccgc ggtctgtgcc ttgatggtga tgccacgttc acgctccaga
     4621 tccatggaat cgagcacctg cgcctccatt tcccgatcgg acagtcctcc gcacatctga
     4681 atgatgcggt cggccagcgt ggatttaccg tggtcaatgt gggcgatgat ggagaaattt
     4741 cggatattgt tcatgggtgc caatagcaag ggcgcctaag gcgccctttt gtatgtaaca
     4801 gactgttagg ccgcggattt taccggaaac cggcaaactc cgcacgcccg cgcttaagaa
     4861 ttatcgttgc ataaaattac tggcagctga cgccatggca agggatgact gaaaaagacg
     4921 cggaagcata taatgctcga ttgaataaca gcagtttatc gaagaaatca tggccattac
     4981 cctcaaggac agtaacgata tcgaaaaaat gcgcgtggcg ggaagactgg ccgccgaggt
     5041 gctggactac atcacaccct tcgtcaagcc gggtgtcacg accggcgaac tggacaagct
     5101 gtgccacgat tacatggtcg aggtgcaggg ctgcatcccg gcaccgctca actatgcgcc
     5161 gcccggccac cagccctacc ccaagtcgat ctgcacttcg gtgaatcatc aggtctgcca
     5221 cggcgtgcca ggtgagaaaa tcctgaagaa cggcgatatc gtcaacctcg acatcactac
     5281 catcaaagat ggctatcacg gcgataccag ccgtatgttc tgcgtcggca cgccttcggt
     5341 acaggcaaag cgactgtgcg acatcaccca cgaatgcatg tggttgggca tcgcccgggt
     5401 caagtcgggg gccaccctgg gcgacgtcgg ccacgccatc cagcagcatg ccgagaaaaa
     5461 cggctattcc gtcgtgcgcg agttctgcgg tcatggcatc ggcaagaaat tccacgaaga
     5521 cccgcaggtg ctgcactacg gcaagcccga caccggactc aagctgcagg ccgggatgat
     5581 cttcaccatc gaacccatga tcaatgccgg aaaacgcgac attcgccagc tcggcgatgg
     5641 ctggaccatc gtcaccaagg accacagcct gtcggcgcag tgggaacaca cggtgctggt
     5701 cacggcagag ggctacgaag tattgactgt ctccgacggc acacctccgg caccaacatt
     5761 cctccccaga tgacaccgct ggcgccagcc atcatcgcca cctggcgcct gcagctgcaa
     5821 cagcggcaag cattattgcg cgcagaattc gaacgcgatg ccgacaccgc gcgcctgctc
     5881 agatatcact gccgtctggt ggaccagctg ctatgcgata tatggctgga aactggcctg
     5941 tccggcgaac tgtgtatggt ggccgtgggt ggttatgggc gcggcgagct gtttccgcat
     6001 tccgatgtcg atctgctgat cctgctgccg gatggccacg cagaagaatt caattcgcgt
     6061 ctggaaccgc tgatcggcct gttctgggac atcggcctgg cagtcggcca cagcgtgcgc
     6121 tcgttagggg aatgcctgga tgaggctgcc gccgatgtga cggtgcgcac caacctgctg
     6181 gaagcacgct ggctggctgg cgacaggcat gccttccagc gcatgcggaa aaggcttgcc
     6241 gaagccatga acgcgaccga gttcctgcag gccaagcagt tggagcagga gcagcgccac
     6301 atgcgcttca acgatactgc ctacaacctg gaacccaaca tcaaggaaag ccccgggggg
     6361 ctgcgcgacc tgcaaaacgt gctgtggatc agtcaggggc tgggattggg cgacaactgg
     6421 ggagcgctgg cgcgcaacgg cctgattacc gcgccagagg cccgacaaat ccgccgccac
     6481 gagctgttcc tgcaaatgct gcgggtacgc ttgcattacc tggcggggcg gcgcgaggat
     6541 cgcctgctat tcgatttcca gaacgagctg gccgcacagc taggtctggc tggaagttcc
     6601 cgccgcaggg ccagcgaaca actgatgcag caatattacc gcagcgccaa gttcgtccgc
     6661 ctgatgaacg aaattctgct acagaacctg caggaacgca ttgccgaatt tcctcagaaa
     6721 atcattgcca tcaacccccg tttccaggcg cgcaatggcc tgctcgaagc aacatcatcc
     6781 agtctgctgc agcgcgaacc gcgcaccata ctggaaagct tcttgctgct ggaacagcat
     6841 ccggaactca aaggtatgag cgcccccatg ctgcgcacgc tatggcgggt gaagggcctg
     6901 gtcaatcgcg agttccgcag ggatgcgggt aacaagcgcc tgttcctgga aatcctgcgc
     6961 cagccagccg gcgtgaccgt cgcgctgcgc cgcatgaacc gctacggcat actgggccgc
     7021 tacatcccgg ctttcggccg catcagcggg caaatgcagc acgacctgtt ccatgtctac
     7081 actgtggacg agcacattct taacgtgttg agaaatctgc gccgtttcgc cacgccaaaa
     7141 tacggctatg aattcccgtt gtgcagccag ctcatcacca ctttcgacaa gccggaactg
     7201 ttgtatctgg gtgcgctgtt ccacgacatc gccaaggggc gaggcggcga ccattccatg
     7261 ctggggacgg cagatgcaaa acgcttctgc aagcagcatg ggctgccacg gcaagacatc
     7321 gaattggtcg cctggctggt agagaaacac ctgttgatgt ccagcacggc acaaaaatgc
     7381 gacctgaccg acccgtcagt gatcgagggc ttcgcgcgcc agatgcggga tgagtaccat
     7441 ttggtcgcgc tttacctgct gaccgtggca gacatccgcg gcaccagtcc tgctgtgtgg
     7501 aacgcatgga aagccaagct gctggaaaac ctgtttctcg ccacccgccg cctgttgcgt
     7561 gggaccagca tcagcgtcga tgccgaggtt gccgcacgcc agcagcaggc tgcaatcacg
     7621 ctcagccact acgccatcgc ctctgacgca taccaggaat tatggaacaa gctcggccgc
     7681 ggctattttc tgcgtcatga aagccaggac atcgcctggc acacgcgggt gctgctggcc
     7741 cacctcaaca gcacgcagcc catcgtacgc agccgtctca gccccggtgg ggacggtatc
     7801 caggtcctag tttactcgcg cgacagcgac gacctgttcg cgcgtatttg cggctttttc
     7861 gagcgcatca attacaccat cgtcgaggca cgcatccata ccacccgcca cggccacgcg
     7921 ctggacagct tcctggtact ggacgaaaac gaaaaaggcg tgcgctatcg cgacctgctg
     7981 agttatatcg agtacgaact gacgcaaaga atcgccagcc cggaaatgcc acaggagcct
     8041 tcatacggac gcgtcagtcg ccaactcaag catttcccca tgcagcctga catcagcatt
     8101 acgccggaag cgaacggcca gaaccatctg ctttcgatca cggcaggtga ccggcccggc
     8161 ctgctgtcgc gcatctccca tatcttcctg cggcatggcg tatgtctgca taccgcaaaa
     8221 atcaacactc tgggaaatcg ggccgaggat actttcctga tttccggtgg ccaagggaat
     8281 agattgaccg acagcgtcac ccagcacttg aaaaaagaac tgtcggaaac gctatgaaat
     8341 gaaaaaggca cccactgaga tgccttttat tgatttggcg gagagggagg gattcgaacc
     8401 ctcgacagag ataaccctgt gccacctttc cagggtggtg acttaaacca ctcatccacc
     8461 tctccgctag cggacgcgca ttatagccga actagccgcg cattccaaac cggaaaacga
     8521 acgccttcac catcaagccc tcactggggc gcccggatct ccagccagcc ccgtcagaat
     8581 ataggtttcg atgtcacctt tgcccttcac agcaatgatg ccgcgagatt tgacaacaaa
     8641 cttgtctttc aacagttcgt aggtggatgc ggaaatctgg atggtattgg ggagcccgta
     8701 ttgctccatc ctgctggcca tattgacggt gtcgccccat agatcgtagc tgaatttgat
     8761 gctaccgatc acgccagcga tgaccggccc ggtattgatg ccgatgcgca tcttgagtcc
     8821 ccggccattg cttgcggaaa cctccgccag tacgtgctgc atttccagcg ccagccgcgc
     8881 cacggcctcg gcgtggtcaa gtctcggcac cggcgcgcca ccgaccacca tgtaggcatc
     8941 accgatggtc ttgatctttt ccaggccata cttctcggtc agggcatcga acctgcagaa
     9001 cacctggttc agcatatcca ccaacgctgc aggcggcatg ccggaggaaa tatgcgtgaa
     9061 atctaccagg tccgcaaaca ggatcgtgac cgcctcatga ctgtcggcga tacggctttc
     9121 gttgttcttg agacg
//
LOCUS       DAOMFA010000061         8917 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_883_length_8917_cov_4.847439, whole genome shotgun sequence.
ACCESSION   DAOMFA010000061 DAOMFA010000000
VERSION     DAOMFA010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 8917)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8917)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8917
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_883_length_8917_cov_4.847439"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(473..548)
                     /locus_tag="PKW44_04465"
     tRNA            complement(473..548)
                     /locus_tag="PKW44_04465"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(513..515),aa:Lys,seq:ctt)
     gene            complement(671..1942)
                     /locus_tag="PKW44_04470"
     CDS             complement(671..1942)
                     /locus_tag="PKW44_04470"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HQR50871.1"
                     /translation="MAHVVVLGAGIGGMPMAYELREQLRTDDRITVVSDFPYFHFVPS
                     NPWVAVNWRSREDISFPIAPYFARKNIQFLPVGAKRVHPEENRVELADGSSLAYDILV
                     IATGPRLAFEEVEGLGPEGHTHSICHVEHAENAGKAWNEFIADPGPVVVGAVQGASCY
                     GPAYEFAFIMDTDLKRRKLRDRVPMTYVTAEPYIGHLGLGGVGDSKGLLESAMRERHI
                     KWICNAKVSKVEAGKMFVIEHNEDGTPKKEHELPFKYSMMLPAFKGIDAVIGIEGLTN
                     PRGFILIDPYQRNTKFSNIFAVGVCVAIPPVEVTPVPTGAPKTGYMIESMVTAAAHNI
                     RAVLDGKEPDTKPTLNALCLADFGDKGAAFVALPQIPPRNVTWYKMGRWVHWAKVAFE
                     KYFLLKMKRGSTEPVYEKYVLKLLGVNKLKG"
     gene            complement(2147..2404)
                     /gene="grxC"
                     /locus_tag="PKW44_04475"
     CDS             complement(2147..2404)
                     /gene="grxC"
                     /locus_tag="PKW44_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HQR50872.1"
                     /translation="MPSITIYTTATCPYCVHAERLLQSKGVTEIHKVRIDLDPQKRAE
                     MMARTGRRTVPQIYIGERHVGGFDDLRALDQAGELEQLLAG"
     gene            complement(2407..2820)
                     /locus_tag="PKW44_04480"
     CDS             complement(2407..2820)
                     /locus_tag="PKW44_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HQR50873.1"
                     /translation="MKFLTQNLWLIGLAIGSAAMLVWPMLRRGAAGATDVSPSEAVLL
                     LNRENALMLDVRNEAEFATGHIAEASNIPLSQLEERLAELARYKDQPLLVNCQGGVRS
                     ANACAMLKKGGFTRVYNLKGGINAWTQANMPVIRE"
     gene            complement(2851..3054)
                     /locus_tag="PKW44_04485"
     CDS             complement(2851..3054)
                     /locus_tag="PKW44_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HQR50874.1"
                     /translation="MTINRAVRIIAGFFILLSLVLAHINGQADLSHLSWLWFTAFVGA
                     NLLQSGFTNFCPMDKILARCGLK"
     gene            complement(3057..6266)
                     /locus_tag="PKW44_04490"
     CDS             complement(3057..6266)
                     /locus_tag="PKW44_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HQR50875.1"
                     /translation="MNKLGISGRIARFFLQSQLTPLLAILAMLLGLFAGLGTPLEEEP
                     QINVTMANVLVPFPGASAKEVENLISIPAEQVLSRIAGVEHVYSVSRPGQAIITVQYE
                     VGQDQTKALVNLYDTIFSKRDWLSPNLGAGEPIIKPIGIDDVPIVALTLWTADDKRGA
                     FDLERVAHALEIELKRVPGTREVATIGGPDNVVRVLLDADRLSAFGIAAQDVRDALQV
                     ANASQPAGKLVADNREILVQTGTFLANSDDVRKLMIGVHNGKPVFMTDVAQILDGADQ
                     PSRYVWLGKGAASKVQGEYPAVTVSVSKKPGENAVDIAHAITQRVEQLRGTLIPEGVE
                     VTVTRDYGQTANEKAQKLIGKLIFATASVVLLVFFALGRREAVVVGAAVMLTLAATLF
                     ASWAWGFTLNRVSLFALIFSIGILVDDAIVVVENIHRRRQLEPQRPFADIIPEAVDEV
                     GGPTILATFTVIAALLPMAFVTGLMGPYMSPIPINSSFGMLISLAIAFIVTPWFCFRL
                     LGRQHVGAGHGEAVVDDKLHRLFVRMLTPFLNRAAGGRKRRLLGLAVMLLIAFSVSLA
                     AFKLVVLKMLPFDNKSEFQVVVDMPVGTPVEKTAAVLREMGAYLATVPEVTDYEAYAG
                     TASPINFNGLVRQYFLRAMPELGDIQVNLVDKHHRSLKSHEIAGKLPPQLQKVADRHG
                     ADLKVVEVPPGPPVLSPIVAEVYGPDYEGQMGVAKQVRAGFEGNADIVFIDDSVEDHA
                     DKVLLRVLQSKATLMGVSQKDIVETMKMALGGEYVTPTHSPGAKYEVPVRISLPPERL
                     NSLDELLKLRVRARDGALVPLSELVQTDNTVREKTIYHKDLLPVVYVVGDMAGKLDSP
                     LYGMFGIRSAVNGMALAEGGTLGEYFIAQPQDPYAGYAIKWDGEWQITYETFRDMGLA
                     YAVGLILIYLLVVAEFKSYLTPLIIMAPIPLTVIGVMPGHALLGSQFTATSMIGMIAL
                     AGIIVRNSILLVDFINQQVVQGVEFQQAVIRAGSVRAKPIVLTGLAAMIGAFFILDDP
                     IFNGLAISLIFGILVSTLLTLVVIPVLYYAFNYKRLQGV"
     gene            complement(6268..7284)
                     /locus_tag="PKW44_04495"
     CDS             complement(6268..7284)
                     /locus_tag="PKW44_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HQR50876.1"
                     /translation="MKQALTLLMLFPGLAAAALPFATAEVNYRPVDDGYAAEAVVEAG
                     RESTLAAQVAGRILMLSVDAGDAVKQGQLLARIDDSEARNSIAGNQAQVAQAQANLVN
                     AQAQLDRTKQLVARKFMSASAMDKAQADYDAAQAQVAAAKAGAAQSSTTRGFTSITAP
                     FSGLVSERLAQVGEMASLGRPLLTLFDPTTLRVVASIPQENLAEVRKAGRASIEFTAT
                     GKWIESTVVTVLPAADSRTHISQVRIELPKDVTGIYPGMFARAHFSTGQAKKMLLPAG
                     AILRRSEVTGVYVVQGDKIQFRQVRLGKELPSGVEVLSGLEVGERVALEPVKAGIYLK
                     QQAR"
     gene            complement(7402..7722)
                     /locus_tag="PKW44_04500"
     CDS             complement(7402..7722)
                     /locus_tag="PKW44_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HQR50877.1"
                     /translation="MQKTDQTNLIEHDEHIEQASHALKAMSHPLRLKVLCVLGDREMS
                     VQDIVELVGTTQSNISQHLAILREKDVLRTRKDANRVFYRVSDPRTLKLIALMRDVFC
                     GFSK"
     gene            7857..>8917
                     /gene="gpmI"
                     /locus_tag="PKW44_04505"
     CDS             7857..>8917
                     /gene="gpmI"
                     /locus_tag="PKW44_04505"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HQR50878.1"
                     /translation="MKVTPVLLLILDGFGHGEDAADNAIALAHKPNWDALLAKYPHTL
                     INASEHSVGLPAGQMGNSEVGHLNIGAGRVVYQDFERINLAIGNGEFHTNPALREAVQ
                     TAKDNSTALHIFGLLSDGGVHSHEQHLHAMLDMAARAGVSRIYVHAFLDGRDTPPRSA
                     AAYLRRMEDQCARSGAGRIASIVGRYYAMDRDKRWARVEAAYKLIAQGRGEFHSDTAS
                     EALQAAYARGENDEFVKATTVGEAVRVKDGDVIVFMNFRSDRARELTTAFLDPAFDGF
                     AREHQPKLGGYYALTAYGLDSLPIKTAFQPQPIHNGFGEYIAGLGLRQLRIAETEKYP
                     HVTFFFNGGEETVYPGEDR"
BASE COUNT         1799 a         2725 c         2640 g         1753 t
ORIGIN      
        1 ccacacgagc cgccagcgcc tggatggcgc cgtcatttgc caggacttcg gctggaatcc
       61 agtcgatgtc ggcggcatcg ggctttctca ctacctcgaa gccactgcga tggtctggat
      121 catcacggcg ttcacgggcg gctattggaa ccaggcgttc aagctgctga gaaagtgagc
      181 ggcttggtag gacgcttgcg gatattcttc gccgccccgg cattggcttc atcaagctgg
      241 acagcgacaa cccagccgac agccaggtgc gcaaatcacg ctcgcaaaag gccatgtgcg
      301 tttgccaagg cgaagaagtt agcgccgatt tcgagagaaa agagggcaac tgaggatgtt
      361 cccgccaggc tataggaccc tggccgcaca cgcagaatga ccgggaaccc cgcggatcgt
      421 gcgggaagca caaatgaaaa cgcccaactg agaacagttg ggcgctgaat tttggtgggg
      481 gatgacagga tcgaactgtc gacaaacgga ttaagagtcc gctgctctac cagctgagct
      541 aatcccccga ggcgcgccat tatcctgatg cgcattgccc ttgtcaactt gtttgcctgc
      601 caatccgcca gcaggcgctt tcagtcaaac gggtctgact caaagtgccc aaatcgtcat
      661 tgctcgacac tcaacctttt agcttgttga cccccagcag tttgagcacg tacttctcgt
      721 agaccggctc ggtgctgccg cgcttcattt tcaacagaaa gtatttctcg aacgccactt
      781 tggcccagtg cacccagcgc cccattttgt accaggtcac gttgcgcggc ggaatctgcg
      841 gcagggcgac gaaggcggcg cccttgtcac cgaagtcggc caggcagagg gcgttgaggg
      901 tgggcttggt atccggctcc ttgccgtcca gcacggcgcg aatgttgtgc gcggcggcgg
      961 tgaccatgga ttcaatcatg tagccggtct tgggtgcacc ggttggcacc ggagtgactt
     1021 ccaccggcgg gatggcgacg cacacgccga cggcgaagat attgctgaat ttcgtgttgc
     1081 gctggtaggg gtcgatcagg atgaagccgc gtgggttggt caggccctcg atgccgatga
     1141 cggcgtcgat gcccttgaag gcaggcagca tcatggagta tttgaacggt agttcgtgct
     1201 ccttttttgg cgtgccatct tcgttgtgct cgatgacgaa catcttgccg gcttcgacct
     1261 tgctcacctt ggcattacag atccacttga tatggcgctc gcgcatggcg gattccagca
     1321 gccccttgga atcacccacg ccgcccagtc cgaggtggcc gatgtaaggt tcggcggtaa
     1381 cataggtcat tggcacgcgg tcgcgcagct tgcggcgctt gaggtcggta tccatgatga
     1441 aggcaaactc ataagcaggg ccgtagcagg aggcgccctg caccgcacca accacgactg
     1501 ggccgggatc ggcgatgaac tcattccatg ccttgccggc gttctcggcg tgttcgacgt
     1561 ggcagatcga gtgggtatgc ccttcggggc caagaccttc gacctcttcg aacgccaggc
     1621 gcgggccggt ggcgatcacc aggatgtcat aagcgaggct gctgccgtcg gccagttcca
     1681 cgcggttttc ctccggatgc acgcgctttg cgccaaccgg caggaactgg atgttcttac
     1741 gcgcgaagta aggggcaatg gggaaggaaa tatcctcacg gctacgccag ttgaccgcca
     1801 cccagggatt agacggcaca aagtggaaat agggaaagtc ggaaacgacg gtgatgcggt
     1861 cgtccgtccg cagttgttcg cgcagttcgt aagccatcgg catgccgccg atgcccgcgc
     1921 ccagtaccac gacgtgtgcc atgtgatctc ctcgatgaac cagttagggc gcgttcacgc
     1981 tcattttgtc atgcaacttt ggccgtgcat gctcgggttg actatagcaa caaaagatat
     2041 atcagtaaat tctaatttat atgctgccga caagcactcc aggcaggtga gcttgccaac
     2101 tgcgcctcga agcggcccgc caaaacatct tcgatgacat cgagcgctaa ccagccagca
     2161 gttgttccaa ctcgccagcc tggtctaaag cgcgcaaatc atcgaagcca ccgacgtggc
     2221 gctcgccaat ataaatttgc ggtacggtac ggcgaccggt tcttgccatc atttcggcac
     2281 gcttttgcgg gtcgagatcg atacgcacct tgtggatttc cgttacgccc ttactctgca
     2341 gcaggcgttc cgcatgcacg cagtaggggc aggtggcggt ggtatatata gtgatgctgg
     2401 gcaggttcat tctcttatta ccggcatatt ggcttgtgtc caggcgttga tgccaccctt
     2461 gaggttgtag acacgggtaa atccgccttt tttcagcatg gcgcaggcgt tggccgagcg
     2521 cacgccgcct tggcagttga ccagcagcgg ctggtccttg tacctggcca gttcggccag
     2581 acgctcctct agctgggaca gcgggatgtt gctcgcctcg gcaatatggc cggtggcgaa
     2641 ttccgcttcg ttgcgcacgt ccagcatcag ggcgttctcg cggttgagca gcagcaccgc
     2701 ctcgctgggc gagacatcag tagccccagc cgcgccgcgc cgcagcatag gccacaccag
     2761 catggcggcg gaaccgatag ccaggccgat cagccagagg ttttgggtca ggaatttcaa
     2821 atcaagctcc tttgtttatt tgcgtatggt ttatttcaga ccacatctgg ccaggatttt
     2881 atccatcggg cagaagttgg tgaaaccact ctgcaacagg ttagcgccga cgaaagcggt
     2941 aaaccacagc caggacaggt gcgacaggtc tgcctggccg ttaatatggg ctagcaccaa
     3001 cgataaaagg atgaaaaagc cagcgatgat gcgcacagcg cgattgatgg tcatggtcaa
     3061 actccttgca gacgtttgta gttgaaagcg taatacagca ccgggatcac caccagcgtc
     3121 agcagggtgg acaccaggat gccaaaaatc agcgaaatgg cgagcccgtt gaagatcgga
     3181 tcgtccagga tgaagaaagc gccgatcatg gcggccagtc cagtgaggac aataggcttg
     3241 gcacgcacgc taccggcgcg gatcaccgcc tgctggaact caacgccctg caccacctgc
     3301 tggttgatga agtccaccag caggatggaa ttacgcacga tgatgccggc cagcgcgatc
     3361 atgccgatca tggaggtagc agtgaactga gatcccagca gggcatggcc gggcatcacg
     3421 ccgatcacgg taagagggat cggcgccatg atgatgagcg gcgtcaggta ggacttgaac
     3481 tcggccacca ccagcaggta gatcaggatc agccccaccg cgtaagccag tcccatgtcg
     3541 cggaaggttt cgtaggtgat ctgccattct ccgtcccact tgatggcata gccagcatac
     3601 gggtcctgcg gctgggcaat gaaatattcg cccagggtgc cgccttctgc cagcgccata
     3661 ccgttgacgg cggagcggat gccgaacatg ccatataaag gggaatccag cttgccggcc
     3721 atgtccccca ccacgtacac caccggcagc agatccttgt ggtaaatagt cttctcgcgc
     3781 acggtgttgt cggtctgcac cagctcagac aacggcacca gtgcgccgtc tctggcacgc
     3841 acgcgcagct tgagcagttc gtccaggctg ttgagccgct ccggcggcag cgagatacgc
     3901 accggcacct cgtactttgc tcccgggctg tgcgttggcg ttacgtattc gccgcccagc
     3961 gccattttca tggtttcgac gatgtccttc tgcgacaccc ccatcaacgt agctttgctt
     4021 tgcagcacgc gcaacagcac cttgtcggca tggtcttcca cgctgtcatc gatgaacacg
     4081 atgtcggcat tgccttcgaa acctgcgcgc acctgcttgg ccacgcccat ctggccttcg
     4141 taatccggcc cgtagacctc ggccacgata ggcgacaaca ccggcggacc cggcggcacc
     4201 tctaccacct tgaggtcggc gccgtggcga tcggcgactt tctgcagttg cggtggcagc
     4261 tttccggcga tctcgtggct cttgagggag cgatggtgct tgtcgaccag gttcacctgg
     4321 atgtcgccca gctccggcat ggctcgcagg aagtactgcc gcaccaaacc gttgaagttg
     4381 atgggcgagg cggtgccggc gtaagcctcg tagtcggtca cttccggcac ggtggcgagg
     4441 taggcgccca tctcgcgcag taccgctgcg gttttttcca ccggggtgcc gaccggcatg
     4501 tccaccacta cctggaattc cgacttgttg tcgaacggaa gcatcttcag caccaccagc
     4561 ttgaatgcgg cgagcgacac cgagaaagcg atcagcagca tgacggccaa gcccagcagg
     4621 cggcgcttgc gcccgcctgc ggcgcgattg aggaagggcg tcagcatcct tacgaacagg
     4681 cggtgtagct tgtcgtccac caccgcttcg ccatgcccgg cacccacatg ctggcggccc
     4741 agcaaccgga agcagaacca aggcgtgacg atgaaggcga tagccaggga aatcagcatg
     4801 ccgaagctgg aattgatcgg gatggggctc atgtacggac ccatcaggcc agtgacgaag
     4861 gccatcggca gcagcgcagc gatcaccgtg aacgtcgcga ggatggttgg cccgccgact
     4921 tcgtctaccg cctcagggat gatgtcggcg aatggccgct gcggctccag ctggcggcgg
     4981 cggtggatgt tctccacgac gacgatcgca tcgtctacca ggatgccgat ggaaaaaatc
     5041 agcgcgaaca gcgacacccg gttaagcgtg aagccccacg cccaggaagc gaacagcgtg
     5101 gcggccagcg tcagcatcac cgccgcgccc actaccacgg cctcacgccg gcccagcgcg
     5161 aaaaatacca gcagcaccac ggaggcggtg gcaaaaatga gcttgccgat cagcttctgt
     5221 gccttttcgt tggctgtctg cccataatcg cgcgtgaccg tgacttcgac gccctccggg
     5281 atcagtgtgc cccgcaattg ctcgactcgc tgggtgatgg cgtgggcgat atccaccgcg
     5341 ttctctcccg gcttcttgga aacggacacc gtcaccgccg ggtactcgcc ctgcaccttc
     5401 gatgccgcgc ccttgcccag ccagacgtag cgtgacggct ggtcagcgcc gtcgagaatc
     5461 tgcgcgacgt cggtcatgaa taccggcttg ccgttgtgca cccctatcat cagctttctg
     5521 acgtcgtcgc tgttagcgag gaaggtgccg gtctgcacca ggatttcacg gttatccgcc
     5581 accagcttcc cggccggctg cgatgcgtta gccacctgca acgcatcgcg tacgtcctgc
     5641 gcggcgatgc cgaaggcaga cagcctgtca gcatccagca gcacgcgcac cacattgtcc
     5701 gggccgccga tggtagccac ttcgcgcgtg ccggggacgc gcttcagctc gatttccagc
     5761 gcatgcgcca cgcgctccag atcgaaggcg ccgcgcttgt cgtccgccgt ccacagcgtc
     5821 agcgcgacga tgggcacgtc gtcgatgcct ataggcttga taatcggctc acccgcgccc
     5881 agattgggcg acaaccagtc acgcttggaa aagatggtgt catagaggtt gaccagcgct
     5941 ttggtctggt cctgcccgac ttcgtactgc acggtgatga tggcctggcc ggggcgcgac
     6001 accgaataga cgtgctccac tccagcgatg cgggagagca cctgctccgc tgggatgctg
     6061 atcaggttct ccacttcttt ggccgaggcc ccagggaacg gcaccagcac gttggccatg
     6121 gtaacgttga tctgcggttc ttcctcgagc ggcgtcccca gcccggcgaa caggccgagc
     6181 agcatagcga gtatcgccag cagcggcgtt agttgcgatt gcaggaaaaa acgcgcgatg
     6241 cggccggaaa taccgagctt gttcacgtca gcgtgcctgc tgtttcagat aaatccctgc
     6301 cttgaccggc tccaatgcaa ccctttcgcc aacctccagg cccgaaagca cctcgacgcc
     6361 cgagggcagt tccttgccca gtcgcacctg gcgaaactgt atcttgtcgc cctgcaccac
     6421 gtagacgccg gtgacttcgc tgcggcgcag gatggcgccg gccggcagca gcatcttttt
     6481 cgcctgacca gtggagaagt gcgcgcgggc gaacatgccg gggtaaatgc cggtcacgtc
     6541 cttgggcaat tcgatgcgca cctgcgaaat atgcgtgcgg ctgtcggcgg ccggcagcac
     6601 cgttaccacc gtgctttcta tccatttgcc ggtggccgtg aactcaatgc tcgcacggcc
     6661 ggccttgcgc acttcggcca gattttcctg tgggatggaa gcgactacac gcagcgtggt
     6721 ggggtcgaac agggtcaaca gcgggcgacc cagactggcc atttcgccca cctgcgccag
     6781 gcgctcggag accaggccgc taaacggcgc cgtaatggat gtaaagccgc gcgtagtgga
     6841 ggactgtgcc gcgccggcct tggcggcggc gacctgtgcc tgcgccgcat cgtaatctgc
     6901 ctgcgccttg tccatggcgg aagcgctcat gaatttgcgg gcaaccagct gtttggtacg
     6961 gtcgagctgt gcctgggcat tgaccaggtt ggcctgcgcc tgcgccacct gcgcctggtt
     7021 gccggcgatg ctgttacgcg cttcgctatc gtcaatgcgt gccagtagct ggccttgctt
     7081 gacagcgtca ccggcatcca cgctaagcat caagatgcgc ccggccacct gtgccgccag
     7141 ggtggattcg cgcccggctt ccactaccgc ctcggccgca tagccatcat ccaccgggcg
     7201 gtagttcacc tccgccgtgg caaacggcag tgcagccgca gccaggccgg ggaacagcat
     7261 caacagggtc agcgcttgct tcataatcac cagcatctca agaatgtaaa ttagtatatt
     7321 ctaatatact ggctatttca agccgctgtt gcgcagcgcc actattcccg agggcaaaac
     7381 cagtggcaag cggggtgagt gctatttgct gaacccgcag aacacatcgc gcatcaacgc
     7441 aatcagcttc agcgtacgcg gatcactgac gcggtagaag acgcggttgg catccttgcg
     7501 ggtacgcagc acgtcttttt cccgcaggat ggcgaggtgt tgggaaatgt tactttgcgt
     7561 ggtgccgacc agctcgacga tatcctgtac gctcatttcc cggtcgccca gcacacacag
     7621 gaccttcagc cgcaatggat gcgacatcgc cttgagcgca tgggaggcct gctcgatatg
     7681 ctcgtcatgc tcgatcagat tggtttggtc tgttttttgc atctttgtgc tggaagttcc
     7741 cgataaaccc acgcccaagg gcatggctat atcagggaag tataatgtga aagcccttaa
     7801 aacatcataa aatagcgaaa gtttacgcga tgaaatatca ggaactgcct gccaagatga
     7861 aagtcacccc cgttctactg ctgatcctcg acggcttcgg tcatggcgaa gatgccgccg
     7921 acaacgccat cgcattagca cacaagccca actgggacgc cctcctggcc aagtacccgc
     7981 ataccttgat caacgcctca gaacactcgg ttggcttgcc ggcgggtcaa atgggtaatt
     8041 ccgaggttgg acatctcaat atcggcgccg gtcgcgtggt gtatcaggat ttcgagcgca
     8101 tcaatctggc tatcggcaac ggcgagttcc ataccaaccc ggctttgcgg gaagcggtac
     8161 aaaccgccaa ggacaatagc actgccctgc atatctttgg cctactgtcg gatggtggcg
     8221 tgcacagcca cgagcagcat ctccacgcca tgctggacat ggcagcgcgc gcaggcgtct
     8281 cccgcattta cgtacatgcc tttctggatg gccgcgacac gccgcccaga agcgccgcag
     8341 cctacttgcg gcgcatggag gaccagtgcg ccaggtcagg cgccggccgt atcgccagca
     8401 tcgtcggtcg ctactacgcc atggatcgcg acaagcgctg ggcgcgcgtg gaagccgcct
     8461 acaaactgat cgcgcaaggc cggggcgagt tccattcaga cacggcatcg gaagccctgc
     8521 aagccgctta tgcgcgcggc gagaacgacg aattcgtcaa ggccaccacc gttggcgaag
     8581 cggtgcgcgt gaaagacggc gatgtcatcg tgttcatgaa cttccgctcc gaccgcgcgc
     8641 gcgagttgac cactgctttc ctcgatcccg ccttcgatgg cttcgcccgc gaacaccagc
     8701 ccaaactcgg cggctattac gcgctgaccg cttacgggct ggatagcttg ccgatcaaga
     8761 ccgcattcca gccacaaccc attcacaacg gttttggcga atacatcgcc ggactcggcc
     8821 tcaggcaact acgcatcgcg gaaaccgaaa aatacccgca cgtcaccttc ttcttcaacg
     8881 gcggagagga aaccgtctat cccggtgaag accgcat
//
LOCUS       DAOMFA010000062         8890 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_889_length_8890_cov_6.005093, whole genome shotgun sequence.
ACCESSION   DAOMFA010000062 DAOMFA010000000
VERSION     DAOMFA010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 8890)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8890)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8890
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_889_length_8890_cov_6.005093"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(21..689)
                     /locus_tag="PKW44_04510"
     CDS             complement(21..689)
                     /locus_tag="PKW44_04510"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HQR50879.1"
                     /translation="MTRVDPANPLAVSAVVIDLDGTLLHTAPDLAEAGRRMMLELGLP
                     PVPEATVQTYIGNGVSRLIKRILSGSLDGEPDAALYARAKPIYEKHYAEVVSLHSRPF
                     PDVVEGLGVLKAAGYRLACITNKAEKFTVPLLQDTGLFDYFELILSGDSLPRRKPDPL
                     PLLHACEVFGITPDQMLLIGDSLNDTQAARAAGCHVFCVPYGYNRGRHVNELDLDAVV
                     DSML"
     gene            complement(694..1392)
                     /gene="rpe"
                     /locus_tag="PKW44_04515"
     CDS             complement(694..1392)
                     /gene="rpe"
                     /locus_tag="PKW44_04515"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013149246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HQR50880.1"
                     /translation="MSKQFRIAPSILSANFAKLGQEITDVIASGADIIHFDVMDNHYV
                     PNLTIGPLVCQAIRPLTDAIIDVHLMVKPVDRIIPDFAKAGANIITFHPEGSEHIDRS
                     LALIKDQGCKAGLVFNPATPLDYLDHVMDKIDMVLLMSVNPGFGGQKFIPQALEKLKQ
                     ARARIDAHTEKTGREIWLEIDGGVNTANIAEIARAGADTFVAGSALFGAPKDTDPHRY
                     DSIVADLRAELAKV"
     gene            1499..2743
                     /gene="hemA"
                     /locus_tag="PKW44_04520"
     CDS             1499..2743
                     /gene="hemA"
                     /locus_tag="PKW44_04520"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HQR50881.1"
                     /translation="MHLFTLGVNHTTAPLSIREHVVFHSESLGQALHDLVAHQPVKEA
                     AILSTCNRTELYCNTDQPQAALDWLAQYHRLQPDAIRPYSYTLSSGDAARHAFRVAAG
                     LDSMVLGEPQILGQMKQAVKVAEHAGTLGTLLHKLFQRTFAVAKEVRTTTDIGASSVS
                     MAAASVKLAQRIFGDLKPLKVLFIGAGEMIELCAEHFAAQRPAAIMVANRTLERGAAL
                     ASRFGGKAILLAELPEQLADYDIVVTCTASQLPIVGLGLVERAVKARKRRPMFMVDLA
                     VPRDVETEVAALDDVFLYTVDDLAQIVQEGLGNRQQAATEAEAIIDIRVDNFMHWLQT
                     REAVPTIRALRDQAERVRRHEVEKALKQLARGEDPQRVLEQFSNTLTNKFLHAPSHAL
                     NTTTGDDRDALESLLRQLYQIL"
     gene            2740..3825
                     /gene="prfA"
                     /locus_tag="PKW44_04525"
     CDS             2740..3825
                     /gene="prfA"
                     /locus_tag="PKW44_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HQR50882.1"
                     /translation="MKPSIQKKLAGLSDRLEELNRLLSSPDVTADMDNYRKLSREHAE
                     LGPIVEQYHVYKQIEADIAAAQEMAADPEMREFAQEEIEAGKERLEANALELQKLLLP
                     KDPNDERGIFLEIRAGTGGDESALFAGDLFRMYSRYAERQRWKVEVVSASESELGGYR
                     EIIAKVDGFGAYSKLKFESGGHRVQRVPETETQGRIHTSACTVAIMPEVDEIADVELN
                     PADLRIDTFRASGAGGQHINKTDSAVRITHLPTGIVAECQDGRSQHSNKASAMKVLAA
                     RIKDVQVREQQSREAATRKSLVGSGDRSERIRTYNFPQGRVTDHRINLTLYKIDAIMQ
                     GDMDELVNALVAEHQAEQLAALSEDAI"
     gene            3828..4685
                     /gene="prmC"
                     /locus_tag="PKW44_04530"
     CDS             3828..4685
                     /gene="prmC"
                     /locus_tag="PKW44_04530"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HQR50883.1"
                     /translation="MGLQALLVQARQRLADTLDLPGNEAALEAQILLRHMLGGVSRVW
                     LISNEGQALTGDQQARFDTLLSRRLQGEPIAYILGRREFYGLEFGITPDVLIPRPDTE
                     TLVEAALQRIPEGQPCRVLDLGTGSGAIAIAIAAHRLLAMVTAVERSPAALEVARGNA
                     ARLSAGNLRLLQSNWFSALEGEVFDVIVSNPPYIAAADPHLSQGDLRFEPASALASGC
                     DGLDDIRLIIAQASAYLVLGGWLLLEHGYDQAPRVAALLRDAGFGEIGHAADLAGIAR
                     VTLGRRPEV"
     gene            complement(4693..5262)
                     /locus_tag="PKW44_04535"
     CDS             complement(4693..5262)
                     /locus_tag="PKW44_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR50884.1"
                     /translation="MRTEVHVHGYLELVEGITNRQVEDALRPLLQYLDVEHLKEVQSL
                     EPDQAGFAFNDRNYTLEMCCTLEVGRNFLPSLEQAMQGLGKLVETATAIEIITYHEDE
                     DRDETQLLFVGPTPEAILDAQRRYMLEDVSALLSRHFRQEAVNEVLALVGDLFQREQN
                     TVTKSAIEEEPEEFSALRMPQVPGRHRLH"
     gene            complement(5305..5856)
                     /locus_tag="PKW44_04540"
     CDS             complement(5305..5856)
                     /locus_tag="PKW44_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HQR50885.1"
                     /translation="MSDPSNITPYLGMAPRLGDRVYVHPAAQVIGDVVLGDDASVWPG
                     TVIRGDVNSICIGAGTNVQDLSVLHVSHKSSWDPGGGPLVIGDNVTIGHKVILHGCTI
                     GDECLVGMGAIIMDKVIIEPRVLVGAGCLVPEGKVLESGYLYLGSPARRARPLTEQEL
                     AHFAYSAQHYIRLKNNYLGVPNG"
     gene            5877..7001
                     /locus_tag="PKW44_04545"
     CDS             5877..7001
                     /locus_tag="PKW44_04545"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HQR50886.1"
                     /translation="MTDISSVGIVTPQTAHFSEPLALKSGAVLPAYDLVYETYGALNA
                     DKSNAVLVCHALSGTHHVAGKYSEDDKHPGWWDNMIGPGKPLDTNRFFVIGVNNLGGC
                     HGSTGPASINPATGKRWGSAFPVVTVEDWVQAQARLADHLGVKSFAAVVGGSLGGMQA
                     LQWTLTFPDRIRNALVIASAPNLTAQNMAFNEVARQAIITDPEFHGGDFYGHGVVPRR
                     GLRIARMLGHITYLSDDAMGAKFGRKLKHDEVQYSFDVEFEMESYLRYQGDKFAGEFD
                     ANTYLRMTRALDYFDPAAEYGGDLSSALSNATADFLVVSFTSDWRFSTARSREIVKAL
                     LDNELSVTYAELTAAHGHDAFLMPDQHYHDIVRAYMENVE"
     gene            6998..7633
                     /gene="metW"
                     /locus_tag="PKW44_04550"
     CDS             6998..7633
                     /gene="metW"
                     /locus_tag="PKW44_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019896885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HQR50887.1"
                     /translation="MTHVPAQAHAHFSEAAAERRDFTAIAQWITPGAKVLDLGCGDGA
                     LLGYLRDTLNVRGYGVEKDDANVLACVKNGINVIQMNLEEGLSGFEADSFDVVILSQT
                     LQAVYQTERIVLEMLRVGKEAIVTFPNFGYWKHRLQLFSGGRMPVSRTLPHQWYNTPN
                     VHLCTIADFDAFCRERNIPVSERLVITEGKTIHLFPNLLGNLAMYRFGQVG"
     gene            complement(7790..8089)
                     /locus_tag="PKW44_04555"
     CDS             complement(7790..8089)
                     /locus_tag="PKW44_04555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR50888.1"
                     /translation="MCEYYDDAGRTDAVRFELPKGNYTIHIEWHGEDPPRQVENSGTA
                     ALPRPIDDRLSLAVLPFANMSSDPEQEYFADGFTDCLITEKSRQAFGLTFAMTVL"
     gene            complement(8082..8330)
                     /locus_tag="PKW44_04560"
     CDS             complement(8082..8330)
                     /locus_tag="PKW44_04560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR50889.1"
                     /translation="MNWTQTEIAEAEACLEKILSSALFAQAERQQRFLRYVVTETLSG
                     RGELLKGYTIVTKVFDREQTFDPAFDSIVRVEAAREIV"
     gene            complement(8419..>8890)
                     /locus_tag="PKW44_04565"
     CDS             complement(8419..>8890)
                     /locus_tag="PKW44_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HQR50890.1"
                     /translation="ASGCFLKHVLPVQKVLYALASLMLVVLGLYLAGIWQGVLVLERA
                     GGLLWRRLQPLSRKLLPLHSTPQAFTLGLLWGWLPCGLVYSVLVAALATGSALQGGLL
                     MLAFGLGTLPTLLAMGMAAVRLKALLQNLWVRRLSGLLVLGFGVFGLLRLAVPA"
BASE COUNT         1732 a         2772 c         2689 g         1697 t
ORIGIN      
        1 ttttttatca acttggcttc ctacagcatg ctgtccacca ccgcgtccaa atccagttcg
       61 ttcacgtgac gaccgcgatt atagccatat ggcacgcaaa acacatgaca gccggccgcc
      121 cgcgctgcct gggtatcgtt gagggaatcg ccgatcagca gcatctggtc cggcgtgatg
      181 ccgaacacct cgcaggcatg cagcagcggc agtgggtcgg gcttgcgcct gggcagcgaa
      241 tcgccggaca ggatcagttc aaagtaatcg aacaggccgg tatcctgcaa cagcggcacg
      301 gtgaacttct ctgccttgtt ggtgatgcag gccaggcggt agccggctgc tttcagcact
      361 cccagccctt ccaccacgtc cggaaacggc cggctgtgca ggctcaccac ttcggcgtaa
      421 tgtttctcgt aaatcggctt ggcgcgcgcg tacagcgctg catccggttc gccatccagg
      481 ctgccgctga ggatgcgttt gatgaggcgc gacacgccgt tgccgatgta agtctgcacg
      541 gtcgcctccg gcaccggcgg cagacccagt tccagcatca tacgcctacc cgcctcggcg
      601 aggtcgggcg cggtgtgcag cagcgtgccg tcgaggtcga tgacgacggc gctgacggct
      661 aacgggttgg cagggtcgac gcgtgtcaaa ttattacacc ttggccagtt cggcgcgcag
      721 atcggcgacg atgctgtcgt aacggtgcgg gtcggtatcc ttgggggcgc cgaacagggc
      781 ggagccggcg acgaaggtgt cggcgccggc gcgagcgatc tcggcgatgt tggcagtgtt
      841 gacgccgccg tcgatttcca gccagatttc acggccggtc ttctcggtgt gggcgtcgat
      901 tctggcgcgg gcttgcttga gcttttccag tgcttgcggg atgaacttct ggccgccgaa
      961 gccggggttg accgacatca gtagcaccat gtcgattttg tccatgacat ggtccaggta
     1021 atccaggggt gtggccgggt tgaataccag gccggccttg cagccctgat ccttgatcag
     1081 cgccagcgaa cggtcgatgt gctcggagcc ttccgggtgg aaggtgatga tgttggcgcc
     1141 ggccttggca aaatccggga tgatgcggtc taccggcttg accatcaggt gcacgtcgat
     1201 aatggcatcg gtcagtggac ggatcgcttg gcaaaccagc gggccgatgg tcaggttggg
     1261 tacgtaatgg ttatccatca catcgaagtg gatgatgtcg gcgccggagg cgatgacgtc
     1321 ggtgatttcc tggcccagct tggcgaaatt ggcggaaaga atgctggggg cgatgcggaa
     1381 ttgcttgctc atgatggctc catcggaaag tcgcaaaaat tgcattttaa ccgcaatcgg
     1441 caggctctaa cagcctgaaa ttgttgctta agcacggccg atactggaaa attgcaaaat
     1501 gcatctgttc acccttggcg tcaatcacac caccgccccc ctctccatcc gcgaacatgt
     1561 cgtcttccac agtgagagcc tggggcaggc gttgcatgat ctggttgcac accagccggt
     1621 gaaggaggcc gccatccttt ccacctgcaa ccgcaccgag ctgtactgca acaccgacca
     1681 gccgcaggcg gcgctggact ggctggcgca ataccatcgc ctgcagccgg atgcgatccg
     1741 gccttattct tacaccctgt ccagcggaga tgccgccagg cacgccttcc gcgttgccgc
     1801 cgggctggat tccatggtgc tgggcgaacc gcagatcctc ggccagatga agcaggcggt
     1861 aaaggtcgcc gaacatgccg gcacgttggg tacgctgctg cacaaattat ttcagcgcac
     1921 tttcgccgtg gccaaggaag tgcgcaccac taccgacatt ggcgccagtt cggtatccat
     1981 ggccgctgcc tcggtcaagc tggcgcagcg catcttcggc gacctcaagc cgctcaaggt
     2041 gctgttcatt ggcgccggcg agatgataga actgtgcgcc gaacacttcg ccgcgcaacg
     2101 tccggccgcc atcatggtcg ccaaccgcac tctggagcgc ggcgcggcgc tggcctcgcg
     2161 cttcggcggc aaagccatcc tgctggccga actgccggag caattggccg actacgacat
     2221 cgtcgtcacc tgtaccgcca gccagctgcc catcgtcggc ctggggctgg tcgagcgcgc
     2281 ggtaaaggcg cgcaagcgcc gccccatgtt catggtggac ttggccgtgc cgcgcgacgt
     2341 ggaaaccgaa gtggccgcac tggacgacgt gttcctctat accgtggacg atctcgccca
     2401 gatcgtgcag gaaggcttgg gcaaccgcca gcaggccgcg accgaagccg aagccatcat
     2461 cgacatccgc gtcgacaact tcatgcactg gctgcaaact cgcgaggccg tccccaccat
     2521 ccgcgctttg cgcgaccagg ccgaacgcgt gcgccgtcat gaggtggaaa aagccctcaa
     2581 gcaactggcg cgaggcgaag acccgcagcg cgtgctggag caattcagca acaccctcac
     2641 caacaaattc ctgcatgcgc ccagccatgc gctcaacacc acgactggcg atgatcgcga
     2701 tgccttggag tcgctgctgc gccagcttta ccagatccta tgaaaccctc gatacagaaa
     2761 aaactggcgg gtctgagcga ccgtctggaa gagctcaatc gcctgctgtc cagccccgac
     2821 gtgacggctg acatggacaa ctaccgcaag ctgtcgcgtg agcacgccga gcttggccct
     2881 attgtcgagc agtaccatgt ttacaagcag atcgaagctg acatcgcagc cgcgcaggag
     2941 atggcggcag acccggagat gcgcgagttc gcgcaggaag aaatcgaggc cggcaaggaa
     3001 aggctggaag ccaatgcgct ggaactgcaa aagctgctgc tgcccaagga ccccaacgat
     3061 gagcgcggca tttttctgga aatccgcgcc ggcaccggtg gcgacgaatc ggcgctgttc
     3121 gccggcgacc tgttccgcat gtattcgcgc tacgccgaac gccagcgctg gaaggtcgag
     3181 gtggtgtcgg ccagcgaatc ggaactgggc ggctaccgcg agatcatcgc caaggtcgac
     3241 ggtttcggcg cctattcgaa gctgaaattc gagtccggcg gccatcgcgt gcaacgtgta
     3301 ccggagactg agacgcaggg ccgcatccat acctcggctt gcactgttgc catcatgccg
     3361 gaggtggatg agatcgccga cgtggaactc aacccggccg acctacgtat cgacaccttc
     3421 cgcgccagcg gtgccggcgg ccagcacatc aacaagaccg actccgcagt gcgtatcacc
     3481 cacctgccca ccggcatcgt cgccgaatgt caggacgggc gctcgcagca ttccaacaag
     3541 gcctcggcca tgaaggtact tgcggcccgc atcaaggacg tgcaggtgcg cgagcagcag
     3601 tccagggagg ccgctacgcg caagagcctg gttggcagcg gcgaccgctc cgagcgcatc
     3661 cgcacctaca acttcccgca aggccgcgtt accgaccacc gcatcaacct taccctgtac
     3721 aagatcgacg ccatcatgca aggcgacatg gacgagttgg tcaacgcgct ggtcgccgag
     3781 catcaggccg agcagttggc ggcattgtcg gaagacgcca tctagccatg ggcttgcaag
     3841 ccctgctggt gcaggcgcga cagcggctcg cagacacgct cgacctgccg gggaacgaag
     3901 ctgccctcga ggcgcagata ttgttgcgcc atatgcttgg cggtgtctct cgcgtctggc
     3961 ttatttcaaa cgaagggcaa gcacttaccg gtgatcagca ggcccggttt gacaccttgc
     4021 tgtcgcgtcg tctgcagggc gaacccattg cctacatcct cggacgacgc gagttctatg
     4081 ggctggaatt tggcatcacg cccgacgtgc tgatcccgcg cccggatacg gaaacactgg
     4141 tcgaagccgc gctgcaacgg attcccgaag gtcagccctg ccgggtgctc gatctcggta
     4201 ccggcagcgg cgccatcgct atcgccattg ccgcgcatcg gctgctggcg atggtcacgg
     4261 cagtggagcg ttccccagcg gcgctggagg tggcgcgtgg caatgcggca aggttgtctg
     4321 ccggaaacct gcgcttgctg caaagtaact ggttcagtgc actggaaggc gaagtcttcg
     4381 atgtgatcgt cagcaaccca ccttacatcg cggcagccga cccgcatctg agccagggcg
     4441 acctgcgctt cgagccggcc agcgcgctgg cgtcgggatg cgacgggctg gatgacattc
     4501 gcctcattat cgcgcaagca agtgcctatc tggtgctagg cggctggctg ctgctggagc
     4561 atggctatga ccaggcgcca agggtggcgg ctctgctgag ggacgcgggg tttggagaga
     4621 ttggccatgc ggccgatctg gcagggatag cgcgggtgac gctggggagg cggccggagg
     4681 tttaatgcag gtttaatgca ggcgatgccg tcccggcact tgcggcatcc gcaatgcgga
     4741 aaactcttca ggctcttcct cgatcgctga tttggtcacg gtgttctgct cgcgctggaa
     4801 caggtcgcca accagcgcca gcacttcgtt caccgcctcc tgtctgaaat ggcgcgacag
     4861 cagcgcggaa acgtcttcca gcatatagcg gcgctgtgcg tccagaatgg cttccggcgt
     4921 cgggccgacg aacagcaact gggtttcgtc gcggtcctca tcttcgtggt aggtgatgat
     4981 ttcgatggca gtagcggttt ccaccagctt gccgagcccc tgcatggcct gttccagcga
     5041 tggcaggaaa ttgcggccga cttccagcgt gcagcacatc tccagggtgt agttgcggtc
     5101 gttgaaggca aaaccagcct gatccggctc caggctctgc acttccttca ggtgttcgac
     5161 gtcgaggtat tgcagcaaag gacgcagcgc gtcttccacc tggcggttgg tgatgccctc
     5221 gaccagttcg aggtagccgt gcacatggac ttcggtgcgc atggggattc cttcaattga
     5281 gtttcgaggc gggattatac ccgatcagcc attcggcacg cccaagtagt tgtttttgag
     5341 gcggatgtag tgctgggcgg aataggcgaa atgtgccagc tcctgctcgg tcagcggcct
     5401 tgcccggcgc gctgggctgc caaggtaaag atagccggat tccagcacct tgccttccgg
     5461 caccaggcag ccggcgccga ccagtacacg cggttcgatg atgaccttgt ccataatgat
     5521 ggcgcccatg ccgaccaggc attcatcgcc gatggtgcag ccgtgcagga tcaccttatg
     5581 gccgatggtg acgttgtcgc caatcaccag cggcccaccc cccggatccc agctcgactt
     5641 gtggctgaca tgcagcaccg acaggtcctg tacgttagtg cccgcaccga tgcagatgct
     5701 attgacgtct cctcgtatca ccgtgcccgg ccacacggaa gcgtcgtcgc ccagcacgac
     5761 atcgccgatg acctgcgcag cagggtgtac atagaccctg tcaccaaggc gcggcgccat
     5821 tccaaggtag ggcgtgatgt tggatggatc gctcatgcct ctagtataat cccgccatga
     5881 ctgatatttc ctctgtcggc atcgttaccc cgcagactgc gcacttcagc gaaccactcg
     5941 ccctgaagtc gggcgccgtg ctgccggcct acgatctggt ttacgaaacc tacggcgcgc
     6001 tcaacgccga caagtccaac gccgtgctgg tctgccacgc actgtccggc acgcaccacg
     6061 tcgccggcaa gtacagcgag gacgacaagc accccggctg gtgggacaac atgatcgggc
     6121 cgggcaagcc gctcgacacc aatcgctttt ttgtcatcgg cgtcaataac ctgggcggct
     6181 gccacggctc caccggcccg gccagcatca atccggctac cggcaaacgg tggggctccg
     6241 cctttccggt ggtcacggtg gaagactggg tacaggcgca ggcgcggctg gccgaccatc
     6301 ttggcgtcaa gagttttgcc gccgtagtcg gcggcagcct gggcggaatg caggcgttgc
     6361 aatggacgct cacctttcct gatcgcatcc gaaatgcgct ggtgattgcc tccgctccca
     6421 acctcactgc acagaacatg gcgttcaacg aggtggcgcg ccaggccatc atcaccgacc
     6481 cggagtttca tggcggcgat ttctacgggc acggcgtggt gcctcgccgt ggcttgcgca
     6541 tcgcccgcat gctgggccat atcacctacc tctccgacga tgccatgggc gccaagtttg
     6601 gccgcaagct caagcacgac gaggtccagt acagtttcga cgtggaattc gagatggagt
     6661 cctacctgcg ctaccagggc gacaagttcg ccggcgagtt cgacgccaac acctacctgc
     6721 gcatgacgcg cgcgctggat tatttcgacc cggcggcaga atatggcggt gacctctcat
     6781 cggcgttgtc caatgccacg gcggattttc tggtggtgtc gttcaccagt gactggcgct
     6841 tctcgaccgc aaggtcgcgc gaaattgtca aggcgctgct ggacaacgaa ctgtcggtga
     6901 cctacgccga actgactgca gcgcatggcc acgacgcctt cctgatgccg gatcagcact
     6961 accacgacat tgttcgtgct tatatggaga atgtggaatg acacacgttc ccgcgcaagc
     7021 tcatgctcac ttcagtgagg ccgccgccga acggcgtgac tttaccgcca tcgcccagtg
     7081 gattacgccg ggcgccaagg ttctcgacct cggctgcggt gatggtgctc tgctcggcta
     7141 cctgcgtgac acccttaacg tgcgcggcta cggcgtggaa aaagacgatg ccaatgtgct
     7201 ggcctgcgtc aaaaacggaa tcaacgttat ccagatgaac ctggaagaag gcctgtccgg
     7261 cttcgaggcc gattcgttcg acgtggtgat cctgtcgcag acgctgcaag ccgtttacca
     7321 gaccgagcgc atcgtgctgg aaatgctccg tgtgggcaag gaagccatcg tcaccttccc
     7381 caatttcggc tattggaagc accgcctgca gctgttctcc ggcggccgga tgccggtgtc
     7441 gcgcaccttg cctcaccagt ggtacaacac ccccaacgtg catttgtgca ccattgccga
     7501 ctttgacgct ttttgccgcg agcgcaacat cccagtgagc gagcgcttgg tcatcaccga
     7561 aggcaaaacc atccacttat tcccgaacct gctaggcaac ctggcgatgt accgcttcgg
     7621 gcaggtgggc tgaaccttag ctccggcagt ttttactccc ctttctccca aggcaggcct
     7681 gacaggtcgg gattagtccc gcttgtcagg ctagtgatcc tgtctgatcc atcccgccgc
     7741 tcccaatagt tactaactgt aagaaactac attaatcctt gcggcaagtt cacagtacag
     7801 tcatcgcaaa tgttaggccg aatgcctgac gcgatttctc cgtaatcagg caatccgtga
     7861 aaccgtcggc gaaatattcc tgctcgggat cgctgctcat gttggcgaac ggcagtaccg
     7921 cgagcgacag cctgtcgtcg atcggtcgcg gaagagcagc ggttcctgaa ttctcgactt
     7981 gacggggggg atcttcccca tgccactcga tgtggatcgt atagttgcct ttcggcaact
     8041 cgaatcgcac ggcatcggta cggcctgcgt catcgtagta ttcacacaat ttcccgcgca
     8101 gcctccacgc gcacgatcga atcaaacgcc ggatcaaagg tctgctcgcg gtcaaacacc
     8161 ttggtgacaa tcgtgtagcc cttgagcaat tcgccgcgcc cggaaagcgt ttcggtcaca
     8221 acatagcgca gaaatcgttg ctgccgctcg gcctgcgcaa acagcgcgct tgacaggatt
     8281 ttttcgagac aggcttcggc ttcggcgatc tctgtttgcg tccagttcat gtgggtaata
     8341 ttcagggcat ccaatgcttg tcgtcaagcc gctgaattat gtcatcaaca ttcgttgata
     8401 ttgaacgcac catccgggtc aggccggtac cgcaaggcgc aacaagccga aaactccgaa
     8461 tcccagcacc agcaagccgc tcagccgacg cacccagaga ttctgtagca gcgctttcag
     8521 tctgaccgcc gccatgccca tcgccagcag cgtgggcagg gtgccgaggc cgaaggccag
     8581 catcagcagg ccgccctgca gcgcgctgcc ggtggcaagc gccgcgacca gcaccgaata
     8641 caccagcccg cacggcagcc agccccacag cagccccagc gtgaaggcct gcggagtgga
     8701 gtgcagcggc agcagttttc tggaaagcgg ctgcaaccgc cgccacagca gcccgccggc
     8761 gcgttccagc accagcacgc cctgccagat gccggccagg tagaggccga gcacgaccag
     8821 catgaggctg gccagtgcgt acagcacctt ttgcaccggc agcacgtgct tgagaaaaca
     8881 gccggaggcc
//
LOCUS       DAOMFA010000063         8826 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_898_length_8826_cov_4.827044, whole genome shotgun sequence.
ACCESSION   DAOMFA010000063 DAOMFA010000000
VERSION     DAOMFA010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 8826)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8826)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8826
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_898_length_8826_cov_4.827044"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1664
                     /locus_tag="PKW44_04570"
     CDS             <1..1664
                     /locus_tag="PKW44_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HQR50891.1"
                     /translation="GGRPLHVIEGPLMAGMNVVGDLFGQGKMFLPQVVKSARVMKQAV
                     AHLIPFIEREKQEEEQRTGVVAKPKGKMVIATVKGDVHDIGKNIVSVVLQCNNFEVVN
                     MGVMVPATEILAMAKAENADIIGLSGLITPSLEEMAHVAREMQRDPYFRSVKMPLLIG
                     GATTSRAHTAVKIAPHYDGPVVYVPDASRSVAVMQSLLTPEQRESYIDEIATDYERVR
                     TQHANKKGTPLLSLAEARENRMQVAFSGERAPVRPKFIGRRELKNIDLALLAQYIDWG
                     QFFQPWDLAGAFPGILEDKVVGEAARKVYGEAQAMLKKIIDGRWLTANGVLALLPANS
                     VRDDDIEIYTDDSRSEVAFTWYGLRQQSVKPAIDGVQRPNQCLADFIAPKGTPDYIGI
                     FAVTAGLGIDKHEQRFQNAHDDYNSIMLKSLADRLAEAFAEYLHQRVRKDFWGYAADE
                     QLSNEELIKEKYCGIRPAPGYPACPDHTVKRDMFRLMQCEKVGITLTESLAMLPAAAV
                     SGFYLSHPESRYFSVDKIGQDQLQDMAARRGVDKEYLQRWLAPNL"
     gene            1731..3104
                     /gene="mpl"
                     /locus_tag="PKW44_04575"
     CDS             1731..3104
                     /gene="mpl"
                     /locus_tag="PKW44_04575"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HQR50892.1"
                     /translation="MHIHILGICGTFMGGIAALAQTAGHRVTGCDANVYPPMSTQLEA
                     QGMELIEGFGVEQLALKPDVFVIGNVVTRGNPLMEEILNRGLPYISGPQWLAENVLQD
                     KWTLAVAGTHGKTTTSSMLAWVLEYAGLSPGFLIGGVPENFGVSARLPVAPRQEPGGQ
                     SPFFVIEADEYDTAFFDKRSKFVHYHPRTVVLNNLEFDHADIFPDLAAIETQFHHLVR
                     TVPGNGLIVANGREGSLDRVLQRGCWTPVERFGTNDGWQAGVPGADGSFDVLLDGNPQ
                     GRAQWELLGEHNRMNALAVLAAARHVGVPVEIGIAALGEFRNVKRRMEIRGTVNGITV
                     YDDFAHHPTAIQTTIAGLRAKVGSARVLAVLEPRSNTMKLGVMKAALPVSLHDADRVF
                     CYAANLGWDAAAALRPLGDKSTTRDDLGALVEMIAAEVHPGDHILVMSNGSFGGIHEK
                     LLARLAS"
     gene            3167..4057
                     /locus_tag="PKW44_04580"
     CDS             3167..4057
                     /locus_tag="PKW44_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HQR50893.1"
                     /translation="MHAVILAIKFAPEIQKQIKDNLPALEVVLVNTKSQSQPEKAEAL
                     AQANLDRGGNTDDDRRLKSPLPVPKNQPLEVAARPGAEAGQKATKSAPHQAEEIQVQQ
                     RVAELERQAQEMMTRLQATQAVESKPVQTNAAPKPENGRQENMPTTTNTADLAARSLE
                     AARLEAEISKDWDNYQKWPKRKFVGARVREYRFASYVESWRQKVEKIGNLNYPEAAKE
                     QKLYGQLRMTVNIKADGSIESIELNESSGHQVLDEAARRIVEMAAPYAAFPADISKDT
                     DILSITRTWTFTREDTLASQ"
     gene            4067..4381
                     /locus_tag="PKW44_04585"
     CDS             4067..4381
                     /locus_tag="PKW44_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HQR50894.1"
                     /translation="MDSSYYRYHVFFCLNRRDDGSPCCCDHDAEGMFDYAKKRVKALG
                     LNGAGKVRINRAGCFDRCAEGPLLVVYPEAVWYTFIDQADIDEIIESHLQNGKVVERL
                     IV"
     gene            complement(4378..4776)
                     /locus_tag="PKW44_04590"
     CDS             complement(4378..4776)
                     /locus_tag="PKW44_04590"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011310731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HQR50895.1"
                     /translation="MACELCANLGGELIWQNDFCRVALVDDTFYPGFCRVILNRHIKE
                     MTELDEGERSRLMAVVFAVESAVRDVLKPDKINLASLGNVVPHLHWHVIPRFADDRHF
                     PNPIWGEPKRDGQSHPLDRQGLIQAIRSRL"
     gene            complement(4783..5916)
                     /locus_tag="PKW44_04595"
     CDS             complement(4783..5916)
                     /locus_tag="PKW44_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HQR50896.1"
                     /translation="MNALACYAANPQQSRGRKHPEVPPQGRNEFQRDRDRIIHSTAFR
                     RLEYKTQVFVNHEGDLFRTRLTHSLEVAQIGRGIARNLGLHEDLVEAIALAHDLGHTP
                     FGHAGQDALNECMREYGGFEHNLQSLRVVELLEQRYAEFDGLNLTFEVREGILKHCSQ
                     QNARQLGELGERFLNKQQSSLEAQVTNLADEIAYNNHDVDDGLRSGLITLEQLSEVEA
                     FARHLHEVRQLYPQIAGRRLVHETVRRMINSQVADLCQQSTSNIAVAQPGDINAVRQR
                     DAALIAFSPEMQRQQRELKQFLRANLYRHYRVNRMSSKAQRIIRELFTAFMRDPGLLP
                     EDFQARDTADQPRAVADYIAGMTDRYASREYRRLFSVEEESLL"
     gene            complement(5913..6989)
                     /gene="aroB"
                     /locus_tag="PKW44_04600"
     CDS             complement(5913..6989)
                     /gene="aroB"
                     /locus_tag="PKW44_04600"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HQR50897.1"
                     /translation="MQTLTVDLGERSYPIHIGQHLLTRAELILPHLKRKQAAIITNTT
                     VAPLYLEQLAAPLITQGVAVLPIILPDGEQYKNAETLNTVYDALLANRCERSTTLIAL
                     GGGVIGDLTGYAAATYLRGVPFIQIPTTLLSQVDSSVGGKTGINHPRGKNMIGAFYQP
                     QLVLADTATLDSLPERELSAGLAEVIKYGLIRDAEFFDWLEVNMAKLVAREPEALAYA
                     IHRSCANKARVVAADERESGERALLNLGHTFGHAIENGMGYGVWLHGEAVAAGTMLAA
                     DLSRRMGWLNEADIARIGCLFQAANLPTQAPALGVERYLDLMGLDKKVVDGKIRFILL
                     QAIGKAVMTGDFPADALQQTLALE"
     gene            complement(6970..7503)
                     /gene="aroK"
                     /locus_tag="PKW44_04605"
     CDS             complement(6970..7503)
                     /gene="aroK"
                     /locus_tag="PKW44_04605"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020183031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HQR50898.1"
                     /translation="MQVTGNIFLVGLMGAGKTTVGKALAKHLGKTFFDSDHEIESRTG
                     VNIPLIFELEGEAGFRKREASVIEELTHMRDIVMASGGGAVLLKQNRENLSQHGTVIY
                     LRANVAELWLRTRNDKHRPLLQTENPRAKLEQLFAERDPLYCEIADIIADTGGQPVSA
                     IVHHLEEALKQYANTHR"
     gene            complement(7546..>8826)
                     /locus_tag="PKW44_04610"
     CDS             complement(7546..>8826)
                     /locus_tag="PKW44_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ family protein"
                     /protein_id="HQR50899.1"
                     /translation="KPGFNGEKLSLNFQNIEVRSVLQVIADFTGLNVVTSDTVNGSIT
                     LRLKDVPWDQAMDIIMQSKGLSQRKIGNIIWVAPSDELAAKEKQQLEAQQQVEDLEPL
                     YTEVFTLKYQKANDLKAILTDSKQKILSKRGSAVVDPRTNTLFVQDTAKYIAGVQRII
                     NKIDIAVRQVLIESRIVIADTGFSKELGVRFGIVQNPYLVCGQPGNGWNSMISGSTRS
                     NYNTAQTGTTTTPTDLNVNLPATSPTAGSFAFSIFKLPAGILLNLELSAAEADSKSKL
                     ISSPRVITMNQQKATIEQGVEIPYQQASSSGATNVSFKKATLSLAVTPQITPDDKISM
                     DLDVHKDSVGQIYNGVPSINTRQVTTQVQVDNGETAVLGGIYEEAKSESVDKVPFFGD
                     LPVLGHLFKRNSQTAQKNELLIFITPKLMQDSVL"
BASE COUNT         1842 a         2589 c         2648 g         1747 t
ORIGIN      
        1 agggcggccg gccgctgcac gtgatcgaag ggccgctgat ggccggcatg aacgtggtcg
       61 gcgacctgtt cggccagggc aagatgttcc tgccgcaggt ggtaaaatcc gcgcgcgtga
      121 tgaagcaggc ggtggcgcac ctgatcccgt tcatcgaacg ggaaaagcag gaagaggaac
      181 aaagaaccgg agttgtcgcc aagcccaagg gcaagatggt gatcgccacc gtcaagggtg
      241 acgtgcacga catcggcaag aacatcgtct cggtagtgct gcaatgcaac aacttcgagg
      301 tggtcaacat gggggtgatg gtgccggcca cggaaatcct ggccatggcc aaggccgaga
      361 atgccgacat catcggcctc tccggcctga ttacgccgtc cctggaagaa atggcccacg
      421 tcgccaggga aatgcagcgc gacccctatt tccgcagcgt gaagatgccg ctgttgatcg
      481 gcggcgccac cacctcgcgc gcccataccg cagtcaagat cgcgccgcat tacgacggcc
      541 cggtggtcta cgtgccggac gcctcgcgct cggtggcggt gatgcagtcg ctgctgacgc
      601 cggaacagcg cgagtcctac atcgacgaaa tcgccaccga ttacgaacgg gtgcgcaccc
      661 agcacgccaa caagaaaggc acgccgctgc tgagcctcgc cgaggcgcgc gagaacagga
      721 tgcaggtggc gttcagcggt gaacgcgctc cggtcaggcc caagttcatc ggccgcaggg
      781 agttgaaaaa tatcgacctc gccctactag cccagtatat cgactggggc cagttcttcc
      841 agccatggga tctggcgggt gctttccccg gcatcctgga ggacaaggta gtgggcgagg
      901 cggcgcgtaa ggtctacggc gaagcccagg ccatgctgaa gaaaatcatc gacggccgct
      961 ggctgacggc caatggcgtg ctcgccctac tgcctgccaa cagcgtgcgc gacgacgaca
     1021 tcgagattta caccgacgac agccgtagcg aagtcgcctt tacttggtac gggctacgcc
     1081 agcaatcggt gaaacctgcc atcgacggcg tgcagcggcc caaccaatgc ctggcggatt
     1141 tcatcgcgcc caagggcacc cctgactaca tcggcatctt tgccgtcacc gccggcctcg
     1201 gcatcgacaa acacgaacag cgcttccaga atgctcacga cgattacaac agcatcatgc
     1261 tgaagtcttt ggccgatcgc ctggcggaag cctttgccga atacctgcac cagcgggtgc
     1321 gcaaggattt ttggggctat gccgccgacg agcagttgag caatgaggaa ctgatcaagg
     1381 aaaaatactg cggcatccgt ccggcaccgg gctatccggc ctgtccggat catacggtca
     1441 agcgcgacat gttcaggctg atgcaatgcg aaaaggtggg aataacgctg accgagtcgc
     1501 tcgccatgct gccggcggcg gcggtatccg gcttctatct gtcgcacccg gaatcgcgat
     1561 atttcagcgt cgacaagatc ggccaggacc agttgcaaga catggcggcg cggcgcggcg
     1621 tggacaagga atatctgcaa cgctggctcg cacccaacct ttagcacgca tggccgccac
     1681 taggcagcca tgagtttgct acaatcaaca aacgaagctt gatcaagcct atgcatattc
     1741 acattctcgg catctgcggc accttcatgg gcggcatcgc ggcgttagcg caaaccgccg
     1801 gacaccgtgt caccggctgt gacgccaatg tctacccgcc catgagcacg caattggaag
     1861 cgcaaggcat ggaactgatc gagggcttcg gcgtcgagca gctggcgctc aaaccagacg
     1921 tcttcgtcat cggcaacgtg gtaacgcgcg gcaacccgct gatggaagaa atcctcaacc
     1981 gcggcttgcc ttatatctcc ggcccgcaat ggctggcgga gaacgtactg caggacaaat
     2041 ggacgctagc cgtggccggc acccacggca agaccaccac cagctccatg ctggcctggg
     2101 tgttggaata tgctggtttg agcccgggat ttttgatcgg cggcgtgccg gagaatttcg
     2161 gcgtttccgc gcgcctgccc gtcgcgccgc gccaggagcc gggcgggcaa tcgcccttct
     2221 tcgtcatcga ggccgacgaa tacgacaccg ccttcttcga caaacgctcc aagttcgtgc
     2281 attaccaccc gcgcacggtc gtcctcaaca atctggaatt cgaccacgcc gatatcttcc
     2341 ccgatctcgc cgccatcgaa acccagtttc accacttggt gcgcaccgtg cccggaaacg
     2401 gcctgatcgt cgccaacggg cgggaaggca gcctggatcg cgtactgcaa cgcggctgct
     2461 ggacgccggt ggaacgcttt ggcacgaacg acggctggca ggcgggcgtg ccgggtgcgg
     2521 acggcagctt cgatgtgctg ctggacggca atccgcaagg ccgtgcgcaa tgggaactgc
     2581 tgggcgagca caatcgcatg aacgcgctgg cggtgctggc cgctgctcgc cacgtcggcg
     2641 tgccggtgga gataggcatc gccgcgctgg gcgagttccg taacgtcaag cgccgcatgg
     2701 aaatccgcgg cacggtcaac ggcatcaccg tctacgacga cttcgcccat caccccactg
     2761 ccatccagac caccatcgcc ggcctgcgcg ccaaggtcgg cagcgcacgc gttctggcgg
     2821 tgctggagcc gcgctccaac accatgaagc tgggagtgat gaaagccgcg ctgccagtca
     2881 gcctgcatga tgcagaccgg gtcttctgct acgcggccaa cctgggctgg gatgccgccg
     2941 ccgcactgcg tccgctggga gacaaaagca ccacccgcga cgatctgggc gcgctggtcg
     3001 aaatgatcgc cgcagaagtg caccccggcg accacatact ggtcatgagc aatggcagct
     3061 ttggcggcat ccacgagaag ctgctggcga ggttggctag ttgaaagacc ggcaagctta
     3121 tcttcgaacc cacgctttag gcgtagccct gatattttca ctggccgtgc acgcagtcat
     3181 cctggccatc aagttcgcac cagagatcca gaagcaaatc aaggacaacc tgccggccct
     3241 ggaagtggtg ctggtcaaca ccaaatcgca gtcacagccg gagaaggcgg aggcgctggc
     3301 acaagccaac ctggatcgtg gcggcaatac cgatgacgac cggcgcctga aatcaccgtt
     3361 gccggtaccg aaaaatcagc cgctggaggt tgctgccagg cccggcgcag aagccgggca
     3421 gaaagccacc aaatccgcac cgcaccaagc cgaagaaatt caggtgcagc agcgcgtagc
     3481 cgagctggaa aggcaggcgc aggaaatgat gacgcggctc caggccacgc aggcggtaga
     3541 aagcaagcca gtgcaaacca acgcggcgcc caagccagaa aacgggcgac aagaaaatat
     3601 gccaaccacc accaacaccg ctgatctcgc tgccaggagc ctggaagccg cgcgcctgga
     3661 agcggaaatt tccaaggact gggacaatta ccagaagtgg ccaaagcgca aatttgtcgg
     3721 ggcaagggta cgggaatatc gcttcgcctc ctatgtcgag tcctggcggc aaaaagtcga
     3781 aaaaatcggc aacctgaatt acccggaagc cgccaaagaa caaaagcttt atgggcagct
     3841 gcgcatgacg gtcaatatca aggcggacgg cagcatcgaa agcatagaac tgaatgagag
     3901 ctcaggccac caggtactgg acgaagccgc caggcgcatc gtcgaaatgg cggcacccta
     3961 tgccgcattc cccgctgaca tcagcaagga cactgacatt ctgagcatta cccgaacctg
     4021 gacgtttacc cgtgaggaca ccctggcttc gcaataggag ggcgctatgg actcaagcta
     4081 ttatcgctac cacgttttct tctgcctcaa ccgccgcgat gacggtagcc cttgctgttg
     4141 cgaccatgac gccgaaggca tgttcgacta tgccaagaaa cgggtaaaag ccttggggct
     4201 aaacggtgca ggcaaggtgc gcatcaatcg cgccggctgc ttcgaccgtt gcgcggaagg
     4261 cccgctactg gtggtttacc cggaggcagt ctggtacacc ttcatcgacc aggccgacat
     4321 cgacgagatc atcgaatcgc atctgcaaaa tggcaaggtg gtggaacggt tgatcgttta
     4381 gaggcgggaa cggatcgcct gtattagtcc ctgacggtcg agcggatggc tttgcccatc
     4441 tcgcttaggt tcgccccaga tcgggttggg aaagtgcctg tcatcagcaa aacgcgggat
     4501 aacgtgccag tgcaggtgcg gcaccacgtt gccgaggctg gcaaggttga tcttgtccgg
     4561 ctttagcacg tcgcgcaccg ccgactcaac ggcaaacacc accgccatca gccgtgagcg
     4621 ctcgccttcg tcaagttccg tcatttcctt gatgtgccgg ttgaggatca cgcggcaaaa
     4681 accgggatag aaagtgtcgt ccaccagcgc cacacggcaa aaatcgttct gccagatcag
     4741 ttcgccgcca aggtttgcac agagttcgca ggccatgacc atttacagca aggactcttc
     4801 ttccaccgag aacaggcggc ggtattcgcg gctggcataa cggtcggtca tgccggcgat
     4861 atagtcggcc acagcacgcg gctggtcggc tgtatcccgc gcctggaaat cctctggcag
     4921 aagcccagga tcccgcatga aggcggtgaa tagttcgcga atgatgcgct gcgccttgct
     4981 gctcatgcgg ttgacgcgat agtgacgata gaggttggca cgcaggaact gcttgagttc
     5041 gcgctgctgg cgctgcatct ccgggctgaa ggcgatgagc gcggcatctc tctggcgtac
     5101 ggcgttgata tctccgggtt gcgctacggc gatattagat gtgctttgct gacacaagtc
     5161 ggccacttgc gagttaatca tgcggcgcac ggtttcatgc accaggcggc ggccagcaat
     5221 ctgcggatat agttgcctga cctcgtgcag gtggcgcgcg aaagcttcca cctccgacag
     5281 ttgttccaat gtaatgaggc ccgagcgcag cccgtcgtcg acgtcgtgat tgttgtaggc
     5341 aatctcgtcg gccaggttgg tgacctgcgc ttccagcgat gactgctgct tgttgaggaa
     5401 acgctcgccg agttcgccga gttgccgcgc attctgctgg gagcaatgtt tgaggatgcc
     5461 ttcgcgcacc tcgaaagtca ggttaaggcc gtcgaattcg gcatagcgct gctccagcaa
     5521 ctccaccacg cgcaaggatt gcagattgtg ctcgaagcca ccgtactcgc gcatgcattc
     5581 attgagggca tcctgtccag catggccgaa tggcgtgtgg ccgaggtcat gtgccagtgc
     5641 gatggcttcc accaggtctt cgtgcaggcc aaggttgcgc gcgatgccgc ggccgatctg
     5701 cgctacttcc aggctgtgag taaggcgcgt gcggaacagg tcgccttcgt ggttgacgaa
     5761 cacctgggtt ttatattcca gccggcggaa agcggtggag tggataatgc ggtcgcggtc
     5821 gcgctgaaat tcgttgcggc cttgcggcgg cacttccgga tgctttctgc cgcgcgactg
     5881 ttgcggattt gccgcgtagc aggccaatgc gttcattcca gcgccagcgt ttgctgcagt
     5941 gcgtccgcgg gaaaatcgcc ggtcatgacc gccttgccaa tggcttgcag caggatgaag
     6001 cgtattttgc catccaccac tttcttgtcc agtcccatca ggtcgaggta tcgctccacg
     6061 cccagagcgg gtgcctgtgt tggcagattg gctgcctgga acaggcaacc gatgcgtgct
     6121 atgtcggctt cattcagcca gcccatgcga cgcgacaggt cagccgccag catggtgccg
     6181 gcagccactg cctcgccatg cagccagacg ccatagccca tgccgttctc aatggcgtgg
     6241 ccaaaggtgt ggccgaggtt gagcagggcg cgctcgccgc tttcgcgttc gtcggcggct
     6301 accacccgcg ccttgttggc acaggagcga tggatggcat aagccaaagc ctcgggctcg
     6361 cgcgccacca gcttggccat gttaacctcc agccagtcga agaattccgc atcgcggatc
     6421 aggccgtact tgatgacttc ggccaggccg gcagaaagct cgcgctcggg caggctgtct
     6481 agcgttgcgg tatcagccag taccagctgc ggctggtaga aagcgccgat catgttcttg
     6541 ccgcgcggat ggttgatgcc ggtcttgcca ccaaccgagg aatcgacctg tgacaacaat
     6601 gtagtgggga tctggatgaa cggcacacca cgcaaatagg tggccgccgc atagccggtc
     6661 aggtcgccga tcacgccacc accgagggca atcagcgtgg tgctgcgctc gcagcgattg
     6721 gccagtaggg catcgtaaac cgtattgagc gtctcggcat ttttgtactg ttcaccatcc
     6781 ggcagaatga tgggcagtac tgcaacgcct tgtgtgatta atggtgctgc aagctgctcc
     6841 aggtagagcg gcgccaccgt ggtattggtg atgatggctg cctgcttgcg cttgaggtgt
     6901 ggcaggatca gctctgcccg ggtcagcaga tgctggccga tatggatagg ataggagcgc
     6961 tcgcctaaat caacggtgag tgtttgcata ttgtttcagt gcttcctcaa gatggtgcac
     7021 gatggcactg accggttggc cgccggtatc ggcgatgata tcggcaattt cgcagtacag
     7081 tgggtcgcgc tcggcaaaca gctgttccag tttggcccga gggttctcgg tttgcagcaa
     7141 ggggcggtgc ttgtcgttgc gggtgcgtag ccaaagttcc gccacgttgg cccgcaggta
     7201 gattaccgtc ccgtgctggc tgagattctc tcggttctgc ttcagtagca cggcaccgcc
     7261 accgcttgcc atcacgatgt cgcgcatatg cgtcagctct tcgatgacgc tggcctcgcg
     7321 cttgcggaat cctgcttcgc cctccagctc gaatatcagg ggaatgttga ccccggtacg
     7381 gctttcgatt tcgtgatcgg agtcgaagaa agtcttgccc aagtgctttg cgagcgcctt
     7441 gccaaccgtg gtcttgccgg cgcccatcag tccgaccaga aaaatattgc ccgtcacttg
     7501 catgacgggc attttaaccg atggcggtca gcgaaaggaa tacgcttaca gcacgctgtc
     7561 ctgcatcaac tttggggtaa tgaaaatcag cagttcattc ttctgcgccg tctggctgtt
     7621 acgcttgaac aaatgtccga gtaccggcaa atcgccgaag aacggcacct tgtccaccga
     7681 ttcggactta gcctcttcgt aaatcccgcc cagcacggca gtctcgccgt tatccacctg
     7741 aacctgggtc gtgacctgac gggtgttgat ggagggtacg ccgttataga tctggccaac
     7801 cgagtccttg tgtacatcca gatccatgct gatcttgtca tccggtgtaa tctgcggcgt
     7861 cacggcaaga gataacgtgg cttttttgaa tgacacgttg gtagccccac tgctggaagc
     7921 ctgctgataa ggaatttcca caccttgctc aatagttgcc ttttgctggt tcatggtaat
     7981 gacgcgtggg ctggaaatca gtttgctctt gctgtcagct tcggctgcag aaagttccaa
     8041 gtttagcaat atccccgcgg gcagcttgaa aatggaaaag gcaaagctgc cggcagtcgg
     8101 agaggtggcc ggcagattca cattgaggtc agttggggtc gtggtagttc cggtttgtgc
     8161 cgtgttgtag ttgctacgtg tagagccact aatcatgctg ttccagccat tgccgggttg
     8221 cccgcaaacc aaatacgggt tttgaacaat gccgaacctg acgcccagtt ctttcgagaa
     8281 accagtatcc gctatcacga tccggctttc gatcagtacc tggcgcactg caatgtcgat
     8341 cttgttgatg atacgttgta cgccggcaat atacttggcc gtatcctgca caaacagggt
     8401 attggtgcgt ggatcaacga cagcgctgcc gcgcttggag agtatcttct gcttggagtc
     8461 ggtcaggatg gccttgagat cattggcctt ttgatacttg agcgtgaaca cctcggtata
     8521 aagcggctcc agatcctcga cttgctgctg cgcctcgagc tgctgcttct cttttgccgc
     8581 caactcgtca gatggcgcca cccagatgat gttgcctatt ttacgttgcg acaggccctt
     8641 gctttgcatg atgatgtcca tggcctgatc ccatggcaca tctttcaggc gcagggtgat
     8701 actgccattc accgtatcac tggtcaccac attgaggccg gtaaagtcgg cgatcacctg
     8761 cagtaccgaa cgcacttcga tgttctggaa attaagcgac agcttttcac cgttgaagcc
     8821 aggctt
//
LOCUS       DAOMFA010000064         8797 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_902_length_8797_cov_5.014299, whole genome shotgun sequence.
ACCESSION   DAOMFA010000064 DAOMFA010000000
VERSION     DAOMFA010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 8797)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8797)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8797
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_902_length_8797_cov_5.014299"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..249)
                     /locus_tag="PKW44_04615"
     CDS             complement(<1..249)
                     /locus_tag="PKW44_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018076359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1841 family protein"
                     /protein_id="HQR50900.1"
                     /translation="MFNPSRDQVRQFFFDAWRKYRAGEALTPLEAMALQVMQMHPEYH
                     AVLDAPQRYLEQEYFPEMGETNPFLHMSLNLSILEQLST"
     gene            complement(242..1324)
                     /locus_tag="PKW44_04620"
     CDS             complement(242..1324)
                     /locus_tag="PKW44_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019897344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST C-terminal domain-containing protein"
                     /protein_id="HQR50901.1"
                     /translation="MAVATGLAVGREPYPSLARAAVDQAMQRAGLKVAQGVLLFLTSE
                     FARDPLPSLREAAKAASCTQVMGCSVPGVFTEQDWVLDAPAAAAMVFGDSISLRTNAA
                     PRDGDLLLALAAPSAINITWLAAPGMRFGGVSGDATGQGPFSVWQYGRGTPGGHCEAI
                     LSGTQGAVSASHGVRILSEPMPVTAVQRYDLLSLEGRPALEILQQSYGIVHGGGEALP
                     VHRLMLGIAETPQALICGDYRLIPLVCINEAERSVTVATQLEPGQQVFWLLRETEAAL
                     RDLEETTLRLQERLPRHPDFGLLFSCQGRGPYFYDGVDRDLELLQSMLPGMPLIGFYG
                     NGEIAPVNGVNELLQYSAVLGLFSHV"
     gene            complement(1315..1959)
                     /gene="nth"
                     /locus_tag="PKW44_04625"
     CDS             complement(1315..1959)
                     /gene="nth"
                     /locus_tag="PKW44_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013149050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HQR50902.1"
                     /translation="MNAEQRREIFRRLAVAISNPTTELNHSSTFELLVAVILSAQATD
                     KGVNLATARLFAVANTPQAILGLGIEGLENYIRTIGLYHAKAKNILATCGILVEQHGG
                     EVPDTRAALEKLPGVGRKTANVILNTAFGQPTIAVDTHIFRLGNRIGLAPGKTPLEVE
                     QKLLKGTPKEYLHDAHHLLILHGRYVCLARKPKCGECVIRDLCEYQAREFSPWP"
     gene            complement(1973..2647)
                     /locus_tag="PKW44_04630"
     CDS             complement(1973..2647)
                     /locus_tag="PKW44_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020182877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HQR50903.1"
                     /translation="MNSREIIENGLWKQNPGVVQLLGLCPTLAVTSTAINGFSLGLAT
                     ALVMAASNGSVSPIRKFVPDEIRIPVFILIIAVLVTVIDLGMHAWFHGLYVALGIFIP
                     LIVTNCIVLARVEAFAAKNDTAPSILDGLFMGLGLAMVLTVLGGMREVVGKGTLFSGL
                     DLVFGPAAKQFMLTVVPDYHGFLLAILPPGAFIGLGLLIAGRNWMESRKAAAQPAPPA
                     ALAAGH"
     gene            complement(2640..3290)
                     /gene="rsxG"
                     /locus_tag="PKW44_04635"
     CDS             complement(2640..3290)
                     /gene="rsxG"
                     /locus_tag="PKW44_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HQR50904.1"
                     /translation="MSESVFKHALGTAGIMIVFTVVGTVLLAYTFFTTRELIARSEAE
                     ARMELFRQILPESLHDNDMLQDTLKIPAGGELHNRAETLAYRARLNGQPSAIILETVA
                     PDGYSGDIKLLVAIDVNGAISGVRVLANKETPGLGDYIDIGHSDWIKKNFDGQSLAKT
                     PDDAWRVKKDGGSFDYMAGATITPRAVVKAVHGVLKYYAVHRDELFATPAKEVAHE"
     gene            complement(3302..4321)
                     /gene="rsxD"
                     /locus_tag="PKW44_04640"
     CDS             complement(3302..4321)
                     /gene="rsxD"
                     /locus_tag="PKW44_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013149047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HQR50905.1"
                     /translation="MDNPPYLSGSTSVSVIMLKVLLALVPAIAAYVWVYGSGILVTLT
                     LASLTALASEAIMLKLRKRPVVPFLLDGSALVTAWLLALALPPLAPWWLVVVGTLFAI
                     VIAKQLYGGLGYNPFNPAMIGYAVLLISFPAIMTQWPAPLELAKAHLGFADQLHYIFG
                     RLLPDGVSLDAVSSATPLDTMKTQLKLQHSVVEIARAPIFGALGGKGNEIVVAAYLLG
                     GLYLVQQRIISWHIPAAFLLALAAMAGLFHLAAPAHYAGPVFHLFSGASMLCAFFIAT
                     DPISGPTTPKGKLIFAASIGVLTWLIRTYGGYPDGVAFAVLLMNICVPLIDAYTQPRV
                     FGHSK"
     gene            complement(4321..5961)
                     /gene="rsxC"
                     /locus_tag="PKW44_04645"
     CDS             complement(4321..5961)
                     /gene="rsxC"
                     /locus_tag="PKW44_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018076364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HQR50906.1"
                     /translation="MISKLFKFHGGVHPPEHKLESTTGAIRPLPLGSPLVLPLRQHVG
                     NLPKIRVQAGDRVLKGRLLAEAEGNISAAVHAPTSGTVVSIDEQLIPHPSGLPDVCIT
                     LQPDGEDAWLERKPVDWRRTDRRVLIASLRESGIVGMGGAAFPTQIKLRPSGEPGIKV
                     LIINGAECEPYITCDDLLMRERADQIVKGIEVAQYLLGAERCIVGIEDNKPQAAEAMR
                     AACSRSVIKIEVAVVPTLYPSGDARRLIHLLTHIEVPSDKRSTDVGVQVFNVATVVAV
                     YRYFEFGEPALSRIVTVTGNVRHPGNFEVPLGTSVAKLLEAAGGALSDTNDLIMGGPM
                     MGFSLPSSQVPVTKATNCIIAGAPKLFPALPPALPCIRCARCAEACPVSLQPQELYWF
                     AKSDNFEKARDYQLFDCIECGCCSYVCPSHIPLVQYYRYAKSEIIALDRAKQAADLAR
                     ERNEFRLARIEREKQERAAKHAQKTAGTKAETAGDEAKKAAIAAALERAKAQKAEVVP
                     QNTESLAPAVQAEIAEIDARRGEALAEPEKAAQEKQES"
     gene            complement(5958..6509)
                     /gene="rsxB"
                     /locus_tag="PKW44_04650"
     CDS             complement(5958..6509)
                     /gene="rsxB"
                     /locus_tag="PKW44_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019918964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HQR50907.1"
                     /translation="MLTAILVMAGLALALGVVLGYAALKFKVEGDPLVDKIDAILPQT
                     QCGQCGYPGCKPYATAIAKGEADINQCPPGGEAGMRALADLLGVEPKPLNAEHGLPKP
                     KSVAVIDESLCIGCTLCIQACPVDAILGAAKHMHTVIASECTGCELCVAPCPVDCIDM
                     VPIPEKVDNWKWRYPVFSLKQVA"
     gene            complement(6509..7084)
                     /gene="rsxA"
                     /locus_tag="PKW44_04655"
     CDS             complement(6509..7084)
                     /gene="rsxA"
                     /locus_tag="PKW44_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HQR50908.1"
                     /translation="MQSYILILVSTVLVNNIVLVKILGLCPFMGVSKKLEASMAMGFA
                     TTFVLTVGSGSSYLINEYLLGTDLFYLRTLSFIVVIAAVVQFTEMVVEKTSPVLYQLL
                     GIYLPLITTNCAVLGIPLLNVQANHNFAESLLFGFGGSVGFSLVLVLFASMRERMEAA
                     DVPGPFKGSAIAMVTAGLMSLAFMGFAGLIR"
     gene            7381..8268
                     /gene="dapA"
                     /locus_tag="PKW44_04660"
     CDS             7381..8268
                     /gene="dapA"
                     /locus_tag="PKW44_04660"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HQR50909.1"
                     /translation="MLKGSLVAIVTPMHADGSLDIASLRSLIDFHIEQGTDGIVIVGT
                     TGESPTVDFDEHCLLIRATVEQVAGRIPVIAGTGANCTREAIELTQQAKEAGADACLL
                     VAPYYNRPTQEGLYQHFKTVAEAVDIPQILYNVPGRTGCDIANDTVLRLAAIPNIVGI
                     KDATGSIERGTDLLLRSPLNFAVYTGDDATALALMLLGGHGVISVTANAAPKLMHEMC
                     EAAFSSDLQTARDINRKLFPLHQKLFIEANPIPVKWVLAEMGLIGPSLRLPLTPLSLQ
                     HHDTLRQAMRSAHLIQETA"
     gene            8265..>8797
                     /gene="bamC"
                     /locus_tag="PKW44_04665"
     CDS             8265..>8797
                     /gene="bamC"
                     /locus_tag="PKW44_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HQR50910.1"
                     /translation="MTVRKLLLSTLFVPAILSLAGCDSVPFMDNSSDYKAAGRGKPLE
                     VPPDLTSVSTSDVYTVPGATYSEYSKEQVQQNQEADKILADPGKIKLERAGAQRWLVV
                     PATPEKVWPVVREFWTDMGFAVVVENPQTGVMETQWTDATDLNKDNKGYLDRFQNGLD
                     KMGALNKRIKFRTRFDQ"
BASE COUNT         1851 a         2695 c         2574 g         1677 t
ORIGIN      
        1 ggtggaaagc tgctcgagga tggacagatt caggctcatg tgcaggaagg ggttggtttc
       61 gcccatctcg gggaaatatt cctgctccag gtagcgttgc ggggcatcca gaacggcatg
      121 gtattcggga tgcatctgca tgacctgcag cgccatagct tccagcggcg tcagcgcttc
      181 gccggcgcgg tatttgcgcc aggcgtcgaa aaagaactgc cgcacttggt cgcggctggg
      241 gttaaacatg gctgaacagc cccagtacgg cggagtattg cagcaactcg ttgacgccat
      301 tgaccggcgc gatttcgccg ttgccgtaaa agccaatcag cggcataccg ggcagcatgg
      361 attggagtaa ttccaggtca cgatccacgc cgtcgtaaaa atacgggcca cgcccctggc
      421 aggagaacag caaaccgaaa tccggatgtc tgggcaatct ttcttgtagc cgcagggtgg
      481 tttcttccag atcgcgcagc gcggcttcag tttcccgcaa cagccagaat acctgttgcc
      541 caggctccaa ctgggtggcc acggtgaccg atcgttccgc ctcgttgatg cagaccaggg
      601 gaatcagccg gtaatctccg caaatcagcg cctgcggcgt ttcggcaatg cccagcatca
      661 ggcggtgcac cggcagcgcc tccccgccgc catgcactat gccataagac tgctgcagta
      721 tctccagcgc gggcctgcct tccaacgaca acaggtcata acgctgtact gccgtgaccg
      781 gcatgggttc tgataggatg cgcaccccgt gagaggcgct aacggcgccc tgggtgccac
      841 tcaatatggc ttcgcaatgc ccccccggcg ttccccggcc atattgccag accgaaaatg
      901 gcccctgacc ggtcgcatcg ccggaaacac cgccaaaacg catgcccggg gcagccagcc
      961 aggtaatatt gattgcgctg ggcgcagcca gggccagcag caaatccccg tcccgtgggg
     1021 ccgcattcgt gcgcaagctg atactgtcac cgaataccat ggcagcggca gcgggcgcat
     1081 ccagcaccca atcctgctcg gtaaaaacac caggcacgga acagcccatg acctgggtgc
     1141 agctggccgc cttggcagcc tcgcgcaggc taggcagcgg atcgcgggca aactcggaag
     1201 tcaggaatag cagcacaccc tgcgccacct tgagtccggc gcgttgcatc gcctgatcga
     1261 cggcggcgcg cgcaagggat gggtagggtt cccggccaac cgccagacca gtcgctacgg
     1321 ccatggagaa aactccctgg cctgatattc gcacaaatca cggatcacgc attccccgca
     1381 cttcggcttg cgcgccaggc acacgtaacg gccatgcagg atcaacaggt ggtgggcatc
     1441 atgcagatat tccttcggcg tgcccttgag cagcttctgc tctacttcca gcggcgtctt
     1501 gcctggcgcc agaccgatgc ggttgccgag gcggaaaatg tgggtatcga cggcaatggt
     1561 gggctggccg aaggcagtgt tgaggatgac attggcagtc ttgcgcccca cgcccggcag
     1621 cttttccagc gccgcgcgcg tgtccggcac ctcgccgcca tgctgctcaa ccaggatgcc
     1681 acaggttgcc aggatgtttt tggctttggc gtggtaaagg cctatggtgc gaatgtagtt
     1741 ctccaatcct tcgatgccga gtccgagaat ggcctgcggc gtattggcaa ccgcgaacag
     1801 tctggcggtg gcgagattga cgcccttatc ggttgcctgc gccgacagga tcaccgccac
     1861 caacagctcg aaggtactac tatggttgag ttcggtcgtg gggttagaga tggcgacggc
     1921 aagccggcga aaaatctcgc ggcgttgctc ggcattcatc cgttaacgcg cctcaatgtc
     1981 cggcggccag agcggccggc ggcgcaggct gggcggcggc cttgcggctc tccatccagt
     2041 tgcggccggc gatcagcaat cccaaaccga tgaacgcgcc aggcggcagg atagccagca
     2101 ggaagccgtg gtaatcgggg acgacagtga gcatgaactg cttggccgcc ggaccgaaca
     2161 ccagatccag cccggaaaac agtgtgccct tgccaaccac ctcgcgcatg ccacccagca
     2221 ccgtgagcac catggccaaa cccagcccca tgaacagtcc atccaggatc gagggcgcag
     2281 tgtcgttctt ggcggcgaac gcttccaccc gtgccagcac aatgcagttg gtgacgatga
     2341 gcgggatgaa gatgcccaga gccacataca agccgtggaa ccaggcgtgc atgccgaggt
     2401 cgatcaccgt caccagtaca gcgatgatga ggatgaacac cgggatgcgg atttcgtccg
     2461 gcacgaattt gcggatcggg gacacgctgc cattgctggc cgccatcacc aacgcggtcg
     2521 ccaagcccag gctgaagcca ttgatcgcgg tgctggtcac cgccagcgtc gggcatagcc
     2581 cgagcagttg caccacgccg gggttctgct tccacaggcc gttttcgatg atttccctgc
     2641 tattcatgtg ctacctcctt cgccggcgtg gcgaataatt cgtcgcggtg caccgcgtaa
     2701 tacttcaata caccatgcac cgccttgacc acggcgcgcg gggtgatggt ggcgccggcc
     2761 atatagtcga aactgccgcc gtccttcttc actctccaag cgtcgtcagg agttttggca
     2821 agcgactggc cgtcaaagtt cttcttgatc cagtcgctgt gtccgatgtc gatgtaatcg
     2881 cccaaccccg gcgtttcctt gttcgccagc acgcgcacgc cggagatggc gccgttgaca
     2941 tctatggcga ccagcagctt gatatcgcca ctgtagccgt ccggcgccac ggtttccagg
     3001 ataatcgccg atggttgccc gttgaggcgg gcgcgatagg ccaaggtttc cgcgcggttg
     3061 tggagttcgc cgccggccgg gatttttagc gtgtcttgca acatatcatt gtcgtgcagg
     3121 ctttccggca gaatctggcg gaacagttcc atgcgcgcct ccgcctcgct tctggcgatc
     3181 agttcgcgcg tggtgaagaa ggtgtaagcc agcagcaccg tgccaaccac ggtaaaaacg
     3241 atcatgatgc cagccgtgcc caaggcatgc ttgaaaacag attcgctcat gttgacttcg
     3301 ctcatttgct atgcccgaac actctaggtt gggtataagc atcaatcagc ggcacgcaga
     3361 tgttcatcag cagcacggcg aaggccacgc catccgggta cccgccataa gtgcggatca
     3421 gccaggtcag cacgccgata ctagcggcga atatcagctt acccttgggc gtagttggac
     3481 cactgatggg gtcagtggcg atgaagaacg cgcacagcat agacgccccg ctgaacaggt
     3541 ggaaaaccgg gccggcgtag tgtgccgggg cggccagatg gaacagcccg gccatggccg
     3601 ccaaggccag cagaaacgca gccgggatat gccagctgat gatgcgctgc tgcaccaggt
     3661 acagcccgcc gagcagatag gcggccacca cgatctcgtt gcccttgcct cccagcgcac
     3721 cgaaaatcgg cgcccgtgcg atttcgacga cactgtgttg cagcttgagc tgggtcttca
     3781 tggtgtccaa cggcgtagcc gaactgacgg catccagact caccccgtcc ggcagtaacc
     3841 ggccaaaaat ataatgcaat tgatcagcaa accccagatg cgctttcgcc aactccagcg
     3901 gcgccggcca ttgcgtcatg atcgccggga aagaaatcag cagaaccgcg tagccgatca
     3961 tggccgggtt aaacgggtta tagcccaagc cgccatagag ttgcttggcg atgacaatag
     4021 cgaacagagt acccaccacc accagccacc agggtgccag cggtggcaac gccagcgcca
     4081 gcagccaagc cgtcaccaag gcactgccat ccagcaggaa cggcactact ggccgcttgc
     4141 gcagcttgag catgatagct tcggatgcca atgccgtaag cgaagccagc gtcagcgtga
     4201 ccaggatgcc actgccatac acccagacat aggcggcaat ggccggcacc aaggcgagca
     4261 gcactttcag catgatgacg ctgacgctgg tgctgcctga aaggtagggg gggttgtcca
     4321 tcagctttcc tgcttttctt gtgcggcctt ttctggttca gccaaggcct cgccgcgtct
     4381 ggcgtcaatc tcggcaattt ccgcctgcac cgcgggtgcc aggctctcgg tattttgcgg
     4441 cacaacttcg gccttttgcg ccttggcgcg ctccaaggca gcggcgatgg cagccttttt
     4501 ggcctcgtcg ccggcagtct cggccttggt gccggcggtt ttctgcgcgt gcttggccgc
     4561 gcgttcctgc ttttcgcgct cgatgcgtgc caggcggaat tcgttgcgtt cgcgcgccag
     4621 gtcggccgcc tgcttggccc gatcaagcgc gataatttcg cttttggcgt aacgataata
     4681 ctgcaccagc gggatatggc tggggcagac atagctgcaa caaccgcact cgatgcagtc
     4741 gaatagctgg tagtcacgcg ccttctcgaa attgtccgac ttggcgaacc agtagagttc
     4801 ctgcggttgc aggctgaccg ggcaggcctc ggcacagcgc gcgcagcgga tgcagggcaa
     4861 ggccggcggc agcgcgggga acaattttgg cgcgccagcg ataatacagt tggtagcctt
     4921 ggtcaccggc acctggctag acggcaagct aaaacccatc atgggcccgc ccataatcag
     4981 gtcgttggtg tccgacagcg cgccgccagc ggcttccagc agtttggcca ccgaggtgcc
     5041 aagcggcacc tcgaagttgc cgggatgacg aacattgccg gtcacggtga cgatgcgtga
     5101 caatgccggc tcgccgaact cgaaatagcg atataccgcc accacggtcg ccacgttgaa
     5161 cacctgcacg ccgacatcgg tggaacgctt gtcgctcggc acttcgatgt gcgtgagcag
     5221 gtggatcagg cggcgcgcat caccgctcgg gtagagcgtc ggcaccaccg ccacctcgat
     5281 cttgatcacg ctgcgggaac aggccgcgcg catcgcctcg gcggcctgcg gcttgttatc
     5341 ctcgatgccg acgatgcagc gctccgcacc gagcaaatac tgggcgacct cgatcccttt
     5401 gacgatctgg tcggcacgct cacgcatcag taggtcgtcg caagtgatgt agggttcgca
     5461 ttcagcgccg ttgatgatca gtactttaat gccgggctcc ccgctcggac gcagcttgat
     5521 ctgggtgggg aacgcggcgc cgcccatgcc gacgatgccg gattcacgca gactggcgat
     5581 gagcaccctt cgatcggtcc ggcgccagtc gaccggcttg cgctccagcc aggcatcttc
     5641 gccatcgggt tgcagcgtga tgcatacgtc cggcagcccg gacggatggg gaattaattg
     5701 ctcatcaata gacacaacgg ttccggaagt tggcgcatgc acggcagcgg atatattgcc
     5761 ttcggcctct gccagcaatc ggcccttgag tacgcggtcg cctgcctgca cgcggatttt
     5821 cggcaaattg ccaacatgct ggcgcaatgg cagcaccagc ggagacccca gcggcaaagg
     5881 acggatcgcg ccggtggtgg actccagctt gtgttctggc gggtgcacgc cgccgtggaa
     5941 tttgaatagt ttggaaatca tgcgacctgt ttcaacgaaa acaccgggta acgccatttc
     6001 cagttatcca ccttttccgg aatcggcacc atgtctatgc aatccaccgg gcaaggcgcc
     6061 acacacaatt cgcagccggt gcattctgag gcgatcacgg tgtgcatgtg cttggctgca
     6121 cccaggatgg cgtccaccgg gcaggcctgg atgcataggg tgcagccgat acagaggctt
     6181 tcgtcgatta ccgccaccga tttcggcttg ggcagcccat gctcggcatt gagcggcttg
     6241 ggctccacgc ccagcagatc ggccagtgct cgcatgccgg cttcgccacc cggagggcac
     6301 tggttgatgt cggcctcgcc cttggcgatg gctgtcgcgt atggcttgca gccgggatag
     6361 ccgcactggc cacactgggt ttgcggcagg atggcatcaa tcttgtcgac cagcggatcg
     6421 ccttcgacct tgaacttgag cgcggcataa cccagcacaa cgcccagcgc cagcgccaag
     6481 ccagccatca cgagaatcgc agtcagcatc agcgtatgag tccggcgaat cccatgaaag
     6541 ccaggctcat cagccccgcc gtgaccatgg cgatggccga gcctttgaac gggccgggca
     6601 cgtccgctgc ttccatgcgt tcacgcatgg aagcaaacag caccagcacc agcgagaaac
     6661 ccaccgagcc accgaaccca aacagcagtg actcggcaaa gttatggttg gcctggacgt
     6721 tgagcaacgg gatacccagc acggcacagt tggtggtgat tagcggcagg taaatgccca
     6781 gcaattgata gagcaccggg ctggtctttt ccaccaccat ctcggtaaac tgcaccactg
     6841 cggcgatgac cacgatgaaa gacagcgtgc gcaggtagaa caagtcggtg cccagcaggt
     6901 attcattgat gagataacta ctgccggagc cgacagtcag cacgaaggtg gtggcgaacc
     6961 ccatggccat cgaggcttcc agttttttgg aaacccccat gaaagggcat agccccagaa
     7021 tcttgaccag cacgatattg ttgaccagta ccgtgctgac caagatcagg atatagcttt
     7081 gaatcatgcc gctagcctgt caaataaatg tgcaattatc atcttcgcgg tggtttcgtt
     7141 caacctcaaa ccagatacgt gcatttgatc cagactgaac tttcaaagtc agaacctgac
     7201 taaccagtcg tttgcgacaa gtactatagc gattcgtgac tacgaaacac aaagaatatg
     7261 ttgttctatt tcataatcag ggattattta aacgcgggcg ggcggttaag caaaaaactt
     7321 actgactgac aactttcggt taaaatacca aggtttaaca acttccaatt caggttaaaa
     7381 atgctgaaag gcagtcttgt cgccatcgtt acgcccatgc atgcggatgg ctcgctggat
     7441 atcgcatccc tgcgttcgct gatcgacttt cacatcgagc agggtacgga cggcatcgtc
     7501 attgtcggca ctaccggcga atcccctacc gtggatttcg acgaacattg cctgctgatc
     7561 cgtgccacgg tggaacaggt cgctggccgc atcccggtga tcgccggtac cggcgccaac
     7621 tgcacgcgcg aagctatcga actgacgcag caagcaaagg aggcgggggc tgacgcctgc
     7681 ctgctggtcg ccccatatta caaccgtccc acacaagaag gtctctatca gcatttcaag
     7741 accgtggccg aagcggtaga cattccacaa atcctgtaca acgtacccgg ccgcaccggc
     7801 tgcgacatcg caaacgacac tgtgctgcgc cttgctgcca tccccaacat cgtcggcatc
     7861 aaggatgcca ccggcagcat tgagcgcggc actgacttgc tactaaggtc gccgctgaat
     7921 ttcgccgtct acaccggcga tgacgccacg gcgctggcgt tgatgctgct gggcgggcat
     7981 ggcgtgattt ctgttactgc caacgcggcg ccgaaactga tgcacgaaat gtgcgaagcc
     8041 gccttcagca gcgacctgca aaccgcccgc gacatcaacc gcaagctgtt cccgctgcat
     8101 cagaaactat ttatcgaggc caacccgatt ccggtcaagt gggtgctggc cgaaatgggc
     8161 ctgatcggcc ccagcctgcg cctgccactg accccgctta gcttgcaaca tcacgatact
     8221 ctgcgccagg ctatgcgcag cgcacacctt atccaggaaa ccgcatgact gtacgtaaac
     8281 tgcttctctc aaccctgttc gttccggcca tactctctct tgccggctgc gactccgttc
     8341 catttatgga taattcatcc gactataagg cagctgggcg cggcaagcca ctggaagtgc
     8401 ctcccgacct gacttccgtc tccacgtctg acgtctatac cgtgcccggg gccacgtatt
     8461 ccgaatacag caaggaacag gttcagcaaa accaagaggc tgacaagatt ctggccgatc
     8521 cgggaaaaat caagctggaa cgcgctggcg cccagcgctg gctggtagta ccggccacac
     8581 cggagaaggt gtggccggtg gtgcgcgaat tctggacgga catgggcttt gcggtcgttg
     8641 tcgaaaatcc ccagaccggt gtcatggaaa cgcaatggac ggacgccacg gatcttaaca
     8701 aggataacaa gggctacctc gacaggttcc agaacgggct ggacaagatg ggcgccctca
     8761 ataagcgcat caagttccgc acccgtttcg atcagga
//
LOCUS       DAOMFA010000065         8776 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_905_length_8776_cov_5.529641, whole genome shotgun sequence.
ACCESSION   DAOMFA010000065 DAOMFA010000000
VERSION     DAOMFA010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 8776)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8776)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8776
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_905_length_8776_cov_5.529641"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..654)
                     /locus_tag="PKW44_04670"
     CDS             complement(<1..654)
                     /locus_tag="PKW44_04670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR50911.1"
                     /translation="MPLSLSVPPLDENLSPPAETSAKKIQELIATLPSANPGLAAGMI
                     IEQLHSLNRQQVPADARVKAMELYRPAISEVTLGLARQYCNQPLPLSSSAIESARAAR
                     ALYGELAYGYKHAILAEEQKLFSFGSEKRLARLIQRAMEALGNLLLVAYHSYSTAPAG
                     VWAEMHHLYIHGLQQSLQDIEVDIGYNTSSVNLTYKRALLLPLADLHHLTSTEINWVV
                     "
     gene            776..1285
                     /locus_tag="PKW44_04675"
     CDS             776..1285
                     /locus_tag="PKW44_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HQR50912.1"
                     /translation="MKRWSGFVKTGGCAELFSARTLKSWLAVALVALAVQAQAAEGFS
                     VKDMAGKTHALSAYKGKWVVVNYWATWCPPCLEEIPDLIALHETRKDLAVIGIALEYD
                     NAREVARFVDDNLMSYPVVLGDDKVTAQIGPASVLPTTYIYNPQGKLVKTKRGMITRK
                     DVEQVISEK"
     gene            complement(1296..1595)
                     /locus_tag="PKW44_04680"
     CDS             complement(1296..1595)
                     /locus_tag="PKW44_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3579 domain-containing protein"
                     /protein_id="HQR50913.1"
                     /translation="MSSDINEIIIEGLTRAGKPFRPSDWVDRMCSTYASFGADRKLRY
                     SPYLKPQLLNGVRCLAVDMRLREINPEGFKQLMLFVEENQLNVKDTEGNNISTPI"
     gene            complement(1703..2278)
                     /locus_tag="PKW44_04685"
     CDS             complement(1703..2278)
                     /locus_tag="PKW44_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HQR50914.1"
                     /translation="MRIILMLALAATLSMNLNGCVPVVAGGAAAGGLAAADRRTTGAY
                     VGDEEIEIKALDRINKALGDKIHVNVTSYNGNVLLTGEAVDEASKAKAESIAKEVRDV
                     RSVTNELSIGSASSLTSRSNDAYITSKVKARMVSENLFPANYVKVVTENSVVYLMGMV
                     TRKEAEDAVQIARGTEGVEKVVTVFEYVVVE"
     gene            complement(2278..2868)
                     /locus_tag="PKW44_04690"
     CDS             complement(2278..2868)
                     /locus_tag="PKW44_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020163706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoheptose isomerase"
                     /protein_id="HQR50915.1"
                     /translation="MELTNRITSHFEQSARLQLEAGELLAPAIAAAAEIIVAALLNEN
                     KILACGNGGSASLAQYFAACMLNRFELERPGLAAIALPADNSTLTSIANDSHFDQIFS
                     RQVLALGQSGDVLLALSMSGNSPNVLNAVQAAHEREISIIAFTGVDGGALLELLGTCD
                     IHIGVPHENAARIQEAYIVALHCLCDAVDCLLLGVE"
     gene            complement(2859..3209)
                     /locus_tag="PKW44_04695"
     CDS             complement(2859..3209)
                     /locus_tag="PKW44_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013149106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HQR50916.1"
                     /translation="MNDSGQEAEKLAAIYLQRHGLKLLETNYRCRFGEIDLIMQDGRE
                     VVFVEVRLRSHATFGGAGESITRDKQQKLARTASHYLAAHGSPPCRFDAMLLSAPSTQ
                     RFEWIRNAFDCAWN"
     gene            complement(3206..4843)
                     /locus_tag="PKW44_04700"
     CDS             complement(3206..4843)
                     /locus_tag="PKW44_04700"
                     /inference="COORDINATES: protein motif:HMM:NF016251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HQR50917.1"
                     /translation="MAQRLLALFLLLNCLLPCNAPAAESAKTSTPAAAKPQKSPEQRY
                     LDGLACMQQADTPCAQVALAGINPASPYAKILEAQIAAASQDYDTVLRLLIPLRTDTG
                     LLPQASASLHATLAQAYEIQDNPLRALEQFSRAGSYLSAPADIEANQSQIWQMLAGQP
                     KETLLQMRGESQDTVTQGWIDLALAVSYSPQPEHDLTRWRSAYPGHPASSALLEKISA
                     GATVDAPAPASSVEGKVALLLPLENPAYAAVAEALRGGFLTAFATAKSTAELQVYSTH
                     GNKDQIVPSYQLAVQEGAGYVVGPLVREEVSTLAASGAVTATTLALNQPEGKPVDKLI
                     LFGRPVEAEARQVAGIGRRLGMQSALVVVADTPLAKRMAEAFASEWKAQQGTLDLELA
                     FSANTHLAELKAQAISHPADMIFLAADAEQARRVRPYLDQATPTFGTSHLYDGDAGNP
                     LNLALDAVHFVDMPWLIEPAAPQFAPYRQVALKFPGGEMQRWFALGVDAWGLLSGGSG
                     TPLQGLTGDIRWEQGQPVRELSMAQFRGERVVSEPLP"
     gene            4835..5683
                     /gene="rsmI"
                     /locus_tag="PKW44_04705"
     CDS             4835..5683
                     /gene="rsmI"
                     /locus_tag="PKW44_04705"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HQR50918.1"
                     /translation="MSHSGTLYVVATPIGNLGDITLRALETLKTVDVIAAEDTRHSAH
                     LLAHFGISAKLVALHEHNERRAGEKLLDELRSGKSVALVSDAGTPGISDPGAVLVSMA
                     RQAGLKVVPLPGACAAVTALSAAGLLMPGFLFVGFLPAKSSHRKQVLKTLCSVPVTLV
                     FYEAPHRVLESVADLAETLGGNRSITFARELTKTFETFHACRLDEALDWLRADPNQQR
                     GEFVLLVEGAPVVEEQAVSDEARRILETLLAELPLKQAVKLATDITGAKKNVLYPIAL
                     ELKSNE"
     gene            5676..6710
                     /gene="pyrC"
                     /locus_tag="PKW44_04710"
     CDS             5676..6710
                     /gene="pyrC"
                     /locus_tag="PKW44_04710"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HQR50919.1"
                     /translation="MNKLTITRPDDWHLHLRDGAMMRDVLPDTARRFGRAIVMPNLRP
                     PVTTTRLAEEYRARILAALPAGMAFQPLMTLYLTDNTSAAEIAAAKASGIVHGVKLYP
                     AGATTNSDSGVTDLANCHNALEAMQQHGLPLLVHAEVTDADVDVFDREKVFIDRHMTP
                     LTRDFPALKVVFEHITTRDAAQFVASASANVAATITAHHLLMNRNAMFAGGIRPHHYC
                     LPVLKREEHRQFLVVAAISGSPKFFLGTDSAPHARSAKETACGCAGMYTAHAAIELYA
                     EAFGRAGALDKLEAFASFYGADFYGLPRNTDRMTLIKESWMVPDSLPFGGEVLVPLRA
                     GQDVAWKIEW"
     gene            7085..8272
                     /locus_tag="PKW44_04715"
     CDS             7085..8272
                     /locus_tag="PKW44_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HQR50920.1"
                     /translation="MSQAPRISSFQPPNRILLGPGPSDVHQRVLNAMARPTVGHLDPC
                     FSDMMDEMKTLLRYAFQTENALTFPISAPGSVGMESCFVNLVEPGDKVIVCANGVFGR
                     RMAENVRRTRGELVLVEDAWGKPVDPQKVEDAFKAHPETRILAFVHAETSTGVESDAR
                     LLTEIAHRHGALVIVDAVTSLGCVPLLVDEWGIDAIYSGSQKGLSCTPGLSPASFGER
                     VIEKLKARRVPVQSWFMDLNLVLGYWAEAKRTYHHTAPINALYGLHEALVMLAEEGLQ
                     ASWARHRRNHLALLAGLQALGLELLVEEKARLPQLNAVVVPQGLSEPQVRADMLNLHG
                     IEIGAGLGDLAGKIFRIGLMGNSSTPANVRVCLTALESVLAAQHWQVVPGRALAAAEQ
                     VWA"
     gene            8291..8610
                     /gene="rnpB"
                     /locus_tag="PKW44_04720"
     ncRNA           8291..8610
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PKW44_04720"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
BASE COUNT         1694 a         2590 c         2744 g         1748 t
ORIGIN      
        1 aacgacccag ttgatttcag tggaagtcag atggtggagg tcggccaggg gcagcagcag
       61 cgcccgcttg tatgtcagat tgacgctgga ggtgttgtag ccgatatcaa cctcgatgtc
      121 ctgcagggac tgttgcagac catggatata taaatggtgc atttctgccc aaaccccggc
      181 cggcgcggtc gagtagctgt ggtaagcgac cagcaacaga ttgcccagtg cctccatggc
      241 gcgctggatc agccgggcca gccgcttttc gctgccgaag ctgaacaact tctgctcttc
      301 ggccaggatt gcgtgcttgt agccgtaagc cagttcgcca tacagcgccc gtgcggcgcg
      361 cgccgactcg atggctgaac tggacagcgg caatggctgg ttgcaatact ggcgtgccaa
      421 gcccagcgtg acttcgctga tggccgggcg gtaaagctcc atggctttca cccgggcgtc
      481 agcgggaacc tgctggcgat taagggaatg cagctgttcg ataatcattc ctgcagccag
      541 tccgggattg gcgctgggca gtgtggcgat cagttcctgt attttttttg cacttgtctc
      601 ggcaggcggc gacaggtttt catcaagcgg gggcactgat aggctaagcg gcatggccgg
      661 cactccaaac agaattagta tgatgttaat tacaaacccc ggctttgtca ccttgaagca
      721 aggcaaatgc tagacttggt acgatgagaa atcgctgacg gatgtcgggg tgttcatgaa
      781 acgatggtca ggttttgtca aaacaggggg ttgcgctgag ctgttttcgg cccggacgct
      841 gaagtcatgg ctcgcggtag cactggtcgc actggcagtg caggcgcagg cggccgaagg
      901 gttcagcgtc aaggatatgg cggggaaaac gcatgcactg agtgcttaca agggcaagtg
      961 ggtggtggtt aattactggg ccacttggtg cccgccctgt ctggaagaga tacccgacct
     1021 gatcgccctt cacgagacgc gcaaggatct ggccgtgatt ggtatcgcgc tggagtatga
     1081 caacgccagg gaggttgcca ggttcgtgga cgacaaccta atgtcctatc cggtggtgct
     1141 gggggatgac aaggtgactg cccagatcgg gccggccagc gtgctgccga ccacctatat
     1201 ttacaacccg cagggaaagc tggtcaaaac caagcgcggg atgattaccc gcaaggatgt
     1261 ggagcaggtg atctcggaaa aataaagcta tccgctcaaa tgggggtgga aatgttgttt
     1321 ccctcagtgt ccttgacgtt gagttgattt tcctcgacga acagcatgag ctgcttgaac
     1381 ccctccgggt tgatctcccg cagacgcatg tccaccgcca ggcaacgcac gccgttgagc
     1441 agttgcggct tgagataagg cgaataacgc agtttgcggt ctgctccaaa gctggcgtag
     1501 gtgctgcaca tgcggtccac ccagtcgctc gggcggaaag gcttgcccgc acgcgtcagg
     1561 ccctcgatga tgatttcgtt gatgtcgctg ctcataaaat tatccgtggc aatccaaaaa
     1621 aggcgcgatt atatcctcta agcatctata gtttctgctg gtttcatcgt ccgcggaacc
     1681 ggaaacctgc caagaggttc tcttattcca ccacgacgta ttcaaacacc gtgaccactt
     1741 tttccacgcc ttccgtgccg cgcgcgattt gcaccgcatc ctcggcttcc ttgcgcgtca
     1801 ccatgcccat caggtaaacc accgagtttt ccgtgaccac cttgacgtaa ttggctggga
     1861 acagattttc actgaccatg cgcgccttga ccttggaggt gatgtaggcg tcattgctgc
     1921 gcgaagtcag cgagctggcg ctgccgatgc tgagttcatt ggtgacgctg cgcacgtccc
     1981 gcacctcttt ggcgatactc tccgccttgg ccttgctggc ttcgtcgacg gcctcgccgg
     2041 tcagcagcac gttgccgttg tagctggtga cattgacgtg gatcttgtct cccagcgctt
     2101 tgttgatccg gtccagcgcc ttgatttcga tttcctcatc tcctacatag gcgcctgtgg
     2161 tgcggcggtc ggcagccgcc aggccgcccg ccgcggcacc gccggcaact acagggacgc
     2221 aaccgttcag gttcatggac agggtggcgg ccagcgccag catgagtatt attcgcatca
     2281 ttcaactcct aacaaaagac aatcaacggc atcgcacagg cagtgcaagg ccacaatata
     2341 cgcttcctgg atacgcgcag cgttttcgtg cgggactccg atatgaatgt cgcaggtgcc
     2401 aagaagttcc agcagggcgc cgccgtcgac gccggtaaag gcgatgatgc ttatttcccg
     2461 ctcgtgcgcg gcctggaccg cattcagtac gttcggtgag tttccgctca tgctgagcgc
     2521 cagcagcacg tctcccgact gtcccagcgc cagtacctgt cgggagaaaa tctggtcgaa
     2581 atggctgtcg ttggcgatgg aggtcagtgt ggaattgtct gcgggcaggg cgatggccgc
     2641 cagaccgggg cgctccaatt cgaaacggtt gagcatgcag gcggcgaaat actgtgccag
     2701 cgaggctgat ccgccgttgc cgcaggccag tatcttgttc tcgttcagca gcgcggcgac
     2761 gatgatttcc gcggctgcgg cgatcgccgg tgccagcaat tctccggctt ccagctgcag
     2821 gcgcgcgctt tgttcgaagt ggctggtaat gcggttagtc agttccatgc gcagtcgaaa
     2881 gcgttcctga tccattcgaa gcgttgtgta gagggggcgc tcagcagcat agcgtcgaag
     2941 cggcatggcg gcgatccatg cgctgccagg tagtggctgg cggtacgtgc cagtttttgc
     3001 tgcttgtcgc gtgtgatgct ctccccggcg ccgccgaagg tggcatggct gcgcaggcgc
     3061 acttcgacaa acacgacttc ccggccatcc tgcatgatga ggtcgatttc accaaagcgg
     3121 caacggtaat tggtttccag cagtttgagg ccgtgtcgtt gcaggtaaat ggcggccagt
     3181 ttctcggcct cttgtcccga atcgttcaag gcagcggctc gctgaccacg cgctcgccac
     3241 ggaactgcgc cattgaaagt tcgcgtactg gctggccttg ctcccagcgg atatcgccgg
     3301 tcaagccttg caatggcgtt ccgctaccac cagacaggag accccaggca tccaccccca
     3361 gggcaaacca gcgctgcatc tctccgcctg ggaatttcag ggcaacctgc cggtacgggg
     3421 cgaattgcgg cgctgccggc tcgatcagcc atggcatgtc gacgaaatgg acggcatcga
     3481 gtgccaggtt gagcgggttg ccggcatcgc cgtcgtacag gtgtgaggta ccgaaagtag
     3541 gggtggcctg gtcgagatag ggacgtactc gccgcgcctg ttccgcatcg gcggcgagaa
     3601 aaatcatgtc ggcgggatgc gagatcgctt gtgctttcag ctcggccaag tgagtgttgg
     3661 cagaaaacgc cagctcaaga tcgagcgtgc cttgctgcgc cttccactcg ctggcaaaag
     3721 cctccgccat gcgcttggcc agcggggtat cagctaccac caccagggcg gattgcatac
     3781 cgaggcgacg gccgataccg gctacctggc gcgcctctgc ctccaccggc cggccgaaca
     3841 ggatcagctt gtcgaccggt tttccctcgg gctggttaag cgccagcgtg gttgccgtca
     3901 ctgcccccga agctgccagt gtgctcactt cctcgcgcac cagcggaccg acgacatacc
     3961 ctgcgccttc ctgcacggca agctggtagc tgggaacgat ctggtccttg ttgccgtgag
     4021 tggaataaac ctgtaattct gctgtgcttt ttgccgttgc gaatgccgtc aggaagccgc
     4081 cgcgcagcgc ttcggcgaca gcggcatagg ccgggttttc cagcggcagc agcagggcaa
     4141 ccttgccttc cacggaggaa gcgggggctg gggcatcgac ggtcgcgccg gctgaaattt
     4201 tttccagcag ggcagacgag gccggatgcc ccggataggc gctgcgccac cgcgtgaggt
     4261 cgtgctcggg ctgcggcgag taggacacag ccagcgccaa gtcgatccag ccctgcgtca
     4321 cggtgtcctg gctttcaccg cgcatttgca gcagcgtctc cttgggctgt ccggccagca
     4381 tttgccatat ttgcgactga ttggcttcga tatcggctgg cgcggaaagg taggagccgg
     4441 ctcggctgaa ctgctccagt gcgcgcagcg ggttgtcctg gatttcatag gcttgcgcca
     4501 gggttgcgtg taaactcgca ctggcttgcg gcagcaggcc ggtgtcagtg cgcagcggaa
     4561 tcagcaggcg cagcacggtg tcgtaatcct ggctggcggc ggcgatctgc gcttcgagga
     4621 tcttggcgta cggtgaagcc gggttgatgc cagccagtgc cacttgcgcg cacggcgtat
     4681 cggcctgttg catgcaggcc agtccgtcaa ggtagcgctg ctccgggctt ttctgcggtt
     4741 tggcggcggc tggcgtggac gtcttggcgg actccgctgc cggcgcattg cacggcagca
     4801 ggcaattcaa caacaggaag agggcgagaa gacgttgagc catagcggca cattatatgt
     4861 ggtggccacg ccgattggaa atctgggcga tatcaccctg cgcgcgctgg aaaccctgaa
     4921 gacggtggat gtgatcgcgg ccgaggatac ccgtcatagc gcccacctgc tcgcccattt
     4981 tggcatctcc gccaaactgg tcgctctgca cgaacacaac gagcgtcgtg ccggcgagaa
     5041 actcttggac gaactgcgtt ccggcaaatc ggtggcgctg gtgtcggacg ccggcacccc
     5101 cggcatcagc gaccccggtg cggtgctggt cagtatggcg cggcaggccg gcctgaaggt
     5161 ggttccgctg cccggcgcct gtgccgcagt gacggcgctg tcggcggccg gcctgctcat
     5221 gccggggttc ctgttcgtcg gcttcctgcc ggccaagagt tcccatcgca agcaggtgct
     5281 gaaaacgcta tgctccgtgc cggtgacgct ggtcttttac gaagccccgc accgcgtgct
     5341 ggaaagcgtg gctgacctgg ccgaaacctt gggcggaaac cgtagcatca ccttcgcgcg
     5401 cgaactcacc aagacctttg aaaccttcca tgcctgccgt ctggatgagg cgctggactg
     5461 gctgcgggcc gaccccaacc agcagcgcgg cgaattcgtc ctgttggtgg agggcgcgcc
     5521 tgtcgtcgag gagcaagccg tttcagacga agcccgccgc atcctcgaaa ccctgctggc
     5581 ggaactgccg ctcaaacagg cggtaaaatt ggcgaccgac atcaccggcg cgaaaaagaa
     5641 cgtgctctac ccaatagccc tggaactgaa atcgaatgaa taagctcact atcacccgcc
     5701 ccgacgactg gcacctgcac ctgcgcgacg gcgccatgat gcgcgacgtg ctgcccgata
     5761 ccgcgcgccg cttcggccgc gccatcgtca tgcccaacct gcgcccgccg gtgacgacga
     5821 cccggttggc ggaagagtat cgcgcacgca ttctggctgc gctgccggcc ggtatggcgt
     5881 tccagccgct gatgacgctc tacctcaccg acaacaccag cgccgccgag atcgccgccg
     5941 ccaaggccag cggcattgtg cacggcgtca aactgtatcc ggcgggcgcc accaccaact
     6001 ccgattctgg cgtgaccgac ctcgccaact gccacaatgc gctggaggcc atgcaacagc
     6061 acggtctgcc attgctggtg catgccgagg tgaccgatgc cgatgtcgac gtgttcgacc
     6121 gcgagaaggt attcatcgac cgccacatga ccccgctgac gcgcgacttc ccggcattga
     6181 aagtggtgtt cgagcacatc accacgcgcg acgcggcgca gtttgtcgct agcgcatccg
     6241 ccaatgttgc cgccaccatc accgcacatc acctgctgat gaaccgcaat gccatgtttg
     6301 ctggaggcat ccgcccgcac cactactgcc tgccggtgct gaagcgtgag gagcatcgac
     6361 aattcctggt tgttgccgcc atttccggca gccccaaatt tttcctcggc accgacagcg
     6421 cgccgcacgc cagatcggcc aaggaaactg cctgcggctg cgccggcatg tacactgccc
     6481 acgccgccat cgagctctat gccgaggctt tcgggcgcgc tggcgcgctg gataaactgg
     6541 aagccttcgc cagtttttac ggggcggatt tctatggcct gccacgcaac acggacagga
     6601 tgaccttgat caaggaaagc tggatggtgc cggacagcct gccgtttggc ggggaagttc
     6661 tggtaccgct gcgtgccggc caggatgtgg cgtggaaaat tgaatggtag gacggacaaa
     6721 ggccgataag ccgtacccgc catgcaaaat ccttgggggt tctacccggg gtacaccagc
     6781 gtataggtga agggcttcga ccaggccggg gaatcggcgc tcatgctcag agcggcctac
     6841 gatttgcgcg ccacgggcta gaggagctga gcgcctatgc gcttctggtt cagagtacag
     6901 gggtcaaggg gcccgagtac aacaaaaatg aaaccgatct aagcttggag tgggtgccgg
     6961 cgccagccaa atagcggggt gtgccatcgc cgcatcttgc cagcctgaaa gacctagcgt
     7021 taaactcaac aattatgcga ccgggcatta cagacccgcg cagacattgt ttcgaggaga
     7081 cttcatgagc caggccccgc gtatttcttc cttccagccg cccaaccgca tcctgctcgg
     7141 ccccggcccg tccgacgtgc accagcgcgt gctgaatgcc atggcgcggc ccacggtagg
     7201 gcatctcgac ccgtgttttt ccgacatgat ggacgagatg aagaccctgc tgcgctacgc
     7261 ttttcagact gaaaacgcgc tgaccttccc gatctcggcg ccgggttcgg tgggcatgga
     7321 aagctgcttc gtcaacttgg tggagccggg cgacaaggtg atcgtatgcg ctaacggcgt
     7381 gttcggccgc cgcatggcgg aaaacgtcag gcgcacgcgc ggtgaactgg tgctggtgga
     7441 agacgcctgg ggcaagccag tcgacccgca aaaggtggag gatgccttca aggcgcatcc
     7501 cgagaccaga attctggcgt tcgtgcatgc cgagacctcc accggggtcg aatccgacgc
     7561 caggctgctg accgagatcg cccatcgcca cggcgcgctg gtgatcgtgg atgcggtgac
     7621 ttcgctcggt tgcgtgccgc tgctggtgga cgagtggggc atcgacgcca tttattccgg
     7681 cagccagaag ggcttgtcgt gcacgcccgg cctctctcct gcctcctttg gcgagcgggt
     7741 catcgaaaag ctgaaggcac gccgggtgcc ggtacaaagc tggttcatgg atttgaacct
     7801 ggtgttgggc tactgggccg aggccaagcg cacctatcac cataccgcgc cgatcaatgc
     7861 cctctacggg ctgcacgaag cgctggtgat gctggcggag gaggggttgc aggcttcctg
     7921 ggcgcgccat cgccgcaacc acctggcgct gctggctggg ttgcaggcgc tagggctgga
     7981 attgctggtg gaggaaaagg cgcgcttgcc gcaattgaac gctgtggtgg tgccgcaagg
     8041 gttgagcgaa ccgcaggtgc gtgccgacat gctgaatttg cacggcatcg agatcggcgc
     8101 cgggctgggc gacctcgcag gcaagatttt ccgcatcggc ctgatgggca acagttccac
     8161 gccagccaac gtgagagtat gcctgacagc gctggaatcg gtgcttgcag cgcagcactg
     8221 gcaggtggtg ccgggcaggg ccttggctgc tgccgagcag gtgtgggcct gaactggtag
     8281 aatggtcgcc gaagctggcc agacagtcgc cgcccgtgca agcgggggga ggaaagtccg
     8341 ggctccgcag agcaggatga cggctaacgg ccgtacgcca taagtcgtaa gactgaaaag
     8401 ccttatgaca aaagcgagga acagggccac agagacgagc gtattaagtt acggtgaaac
     8461 gcggtaacct ccatccggag caagaccaaa taggggaaca atggcgtggc tcgcgccgtt
     8521 cccgggtagg ttgcttgagc ccatgagcaa ttgtgggcct agaggaatga ctgtccacga
     8581 cagaacccgg cttatcggcc agcttctttt tctttcaagt ccccgcccac caccagatca
     8641 ggctgttgca aagcactcct tacaagtcac tttcacttag ttttctatcc cattaatttt
     8701 taaggacaat tttttatcac aaaaaaccgc gtaagtcttt gacctgaaaa ggaaaaacat
     8761 caaaaaaggg cttgca
//
LOCUS       DAOMFA010000066         8614 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_937_length_8614_cov_6.005959, whole genome shotgun sequence.
ACCESSION   DAOMFA010000066 DAOMFA010000000
VERSION     DAOMFA010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 8614)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8614)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8614
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_937_length_8614_cov_6.005959"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..709)
                     /locus_tag="PKW44_04725"
     CDS             complement(<1..709)
                     /locus_tag="PKW44_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018229603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bc complex cytochrome b subunit"
                     /protein_id="HQR50921.1"
                     /translation="MSMEKYLTGVLNWVDARFPLTANWKAHLSEYYAPKNFNFWYYFG
                     SLAMLVLVIQIVTGIFLTMHYKPDANLAFASVEYIMRDVSWGWLIRYMHSTGASMFFI
                     VVYLHMFRGLLYGSHRNPRELIWLFGVAIFLCLMGEAFFGYLLPWGQMSFWGAQVIVN
                     LFTSIPFIGRDISLWLRGDFTISDATLNRFFAFHVIALPLVLLGLVAAHIIALHEVGS
                     NNPDGIEIKKHKDPKTGI"
     gene            complement(706..1299)
                     /gene="petA"
                     /locus_tag="PKW44_04730"
     CDS             complement(706..1299)
                     /gene="petA"
                     /locus_tag="PKW44_04730"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045275 - respiratory chain complex III
                     [Evidence IEA];
                     GO_function: GO:0008121 - ubiquinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HQR50922.1"
                     /translation="MANQEVDQSKRRFLVAATAAMGGVATVAVAVPFVKSMLPSERAK
                     AAGAPVEVDVAKIESGAMITVEWQGKPVWIVNRSKEMLELLTNHNDKLTDADCSVPQQ
                     PEYCKAPTRSIKPEYMVVIGICTHLGCSPSPKLKPGEEGMGADWPGGFFCPCHGSKFD
                     LAGRVFQGSPAPTNLIVPQYKYLSDTRVLIGEDGKGA"
     gene            complement(1375..2121)
                     /locus_tag="PKW44_04735"
     CDS             complement(1375..2121)
                     /locus_tag="PKW44_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HQR50923.1"
                     /translation="MKLGELLDYTGQLLQVDRFRDYCPNGLQVEGRSDVGSIVTGVSA
                     SLALLQAAAELGADLVLVHHGWFWKSEDARVTGIKRDRLKFLLQRDISLVGYHLPLDA
                     HPELGNNARLAAVLGFETEGWFGEQPIAAHGRLSQPASLAALGQSVAQKFDRTPLLVG
                     DGSATIRRIAWCSGAAQDYLAQAIALGVDAFLTGEVSEHTVHLARESGVAFIAAGHHA
                     TERYGVQALGEHLAERFGIGHQYIEIPNPV"
     gene            2247..3401
                     /locus_tag="PKW44_04740"
     CDS             2247..3401
                     /locus_tag="PKW44_04740"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HQR50924.1"
                     /translation="MKKLWLVFAQTVTISLAILFVVQTFKPGLLGLGERVIAHQVQGT
                     TPTTETVSSYSSAARKAMPSVVNIFTSKEATAGNPQIMDDPQFRHFFGDQFDDQPQRE
                     NSLGSGVIVSPDGLILTNHHVIESADEIEVALADGRTAPARVVGSDPETDLAVVKVDL
                     KNLPAVTFGQSDKLDVGDVVLAIGNPFGVGQTVTQGIVSALGRNHLGINTFENFIQTD
                     APINPGNSGGALVDASGNLIGVNSVIYSRSGGSMGIGFAIPVSLARQVMEQIIRQGSV
                     TRGWIGIEAQDISPELAESFKLKEARGALIAGVMRGGPADKAGIKPGDVLLEINGNKV
                     MDGSSMLNLVAALKPEHPATLKIARNQTELNLPVVVAKRPKPLTPPREQH"
     gene            complement(3403..4131)
                     /gene="tatC"
                     /locus_tag="PKW44_04745"
     CDS             complement(3403..4131)
                     /gene="tatC"
                     /locus_tag="PKW44_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HQR50925.1"
                     /translation="MSESETFIAHLIELRDRLLRMVVGIIITFICLFPFANRLYNTLA
                     QPLLAKLPAGGQLIATAVTTPFLVPMKVAMMAALVVSLPYTLYQVWSFIAPGLYAHEK
                     RFIAPLVVASTLLFLLGMAFAYFLVFPVVFGFIVSTAPEGVQVMTDIGNYLDFVITLF
                     IAFGIAFEVPIVVVALVKFGLVSVAQLKEARSYVIVGAFILGAIFTPPDVVSQVMLAV
                     PLWLLFEAGVVVAGLIKRDEASQP"
     gene            complement(4128..4427)
                     /gene="tatB"
                     /locus_tag="PKW44_04750"
     CDS             complement(4128..4427)
                     /gene="tatB"
                     /locus_tag="PKW44_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HQR50926.1"
                     /translation="MFDIGFSEILVIAVVALVVIGPERLPKVARTLGLLTGRMQRYVS
                     TVKADIDRELHLDELRKLEGEARHTIASVESQVAAESGDVERTIKSTVDDVAKSP"
     gene            complement(4444..4659)
                     /gene="tatA"
                     /locus_tag="PKW44_04755"
     CDS             complement(4444..4659)
                     /gene="tatA"
                     /locus_tag="PKW44_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043953 - protein transport by the Tat
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HQR50927.1"
                     /translation="MGSFSIWHWLIVLVVVLLIFGTKKLRNIGSDLGGAVKGFKDAMR
                     EEQTTPKVEGRGGRTVEGEVTQKSKSE"
     gene            complement(4670..5005)
                     /locus_tag="PKW44_04760"
     CDS             complement(4670..5005)
                     /locus_tag="PKW44_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HQR50928.1"
                     /translation="MSDCIFCKIANGAIPSKKVYEDDEVLVFHDIHPIAPVHFLLVPK
                     AHVDSLSECELQHQVLLGKLLLLAPRLAAEQGLDGFRTMINTGRSGGQEVFHLHVHVF
                     GGSSTLPKV"
     gene            complement(5007..5327)
                     /locus_tag="PKW44_04765"
     CDS             complement(5007..5327)
                     /locus_tag="PKW44_04765"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018508752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HQR50929.1"
                     /translation="MSDILDRLAGTLEERKSADPQSSYVAKLYAKGSDAILKKVGEEA
                     AETIIAAKGGDRQQIIYETADLWFHCLVMLAHNNLHPSDVLNELARREGLSGLDEKAS
                     RQEG"
     gene            complement(5320..5712)
                     /gene="hisI"
                     /locus_tag="PKW44_04770"
     CDS             complement(5320..5712)
                     /gene="hisI"
                     /locus_tag="PKW44_04770"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HQR50930.1"
                     /translation="MPDSWLNKVNWTHEGLVPVIAQEAGSGQVLMFAWMDREALRLTV
                     ETGQAVYWSRSRKKLWRKGEESGHVQVVKEIRLDCDEDVILLTVEQLGGIACHTGRHS
                     CFFQKLDCRQWVVDQPVLKKPEEIYRYE"
     gene            complement(5712..6470)
                     /gene="hisF"
                     /locus_tag="PKW44_04775"
     CDS             complement(5712..6470)
                     /gene="hisF"
                     /locus_tag="PKW44_04775"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HQR50931.1"
                     /translation="MGLAKRIIPCLDVTDGRVVKGVNFVELRDAGDPVEIARRYDEQG
                     ADELTFLDITASSDNRGLIFHIIEQVASQVFIPLTVGGGVRAVEDVRNLLNAGADKVS
                     INTAAVLNPQLVADAAGRFGSQCIVVAIDAKRVGDHWEVFTHGGRKPTGFDAVEWAKR
                     MVGLGAGELLLTSMDRDGTKSGFDLELTRAISDAVEVPIIASGGVGNLDHLVDGVKRG
                     GADAVLAASIFHFGEYTVRQAKQYMADHGIEVRL"
     gene            complement(6544..7293)
                     /gene="hisA"
                     /locus_tag="PKW44_04780"
     CDS             complement(6544..7293)
                     /gene="hisA"
                     /locus_tag="PKW44_04780"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019919456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HQR50932.1"
                     /translation="MLIIPAIDLKDGHCVRLKQGLMEDATVFSEDPGAMARHWVDKGG
                     RRLHLVDLNGAFAGKPVNEGAIKSIVDAVGGDIPIQLGGGIRDLDTIERYLDDGISYV
                     IIGTAAVKNPGFLHEACDAFPGHIMVGLDAKDGKVAVDGWSKLTGHDVVDLAKRFEDY
                     GVEAVVYTDIGRDGMLSGVNIEATVALAQGLRIPVIASGGITDLDDVKRLCAVEHEGI
                     LGAITGRAIYEGTLDFAAAQTLADELNGITA"
     gene            complement(7311..7952)
                     /gene="hisH"
                     /locus_tag="PKW44_04785"
     CDS             complement(7311..7952)
                     /gene="hisH"
                     /locus_tag="PKW44_04785"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HQR50933.1"
                     /translation="MADIAVIDYGMGNLRSVEQALRHVAGDQSVLVTSDPEEVLAADR
                     VVFPGQGAMPDCVRELDARGLRQPLLVAARNKPFLGICIGLQMLFSHSEEGDTLGLGI
                     FPGRVRRFPSGMHDAAGSKLKVPHMGWNEVRQARSHPLWAGIALDARYYFVHSYYVEP
                     TDPALETAYTEYPSRFTSAVGHDNIFAVQFHPEKSQQAGLSLLYNFVNWDGRP"
     gene            complement(7989..8576)
                     /gene="hisB"
                     /locus_tag="PKW44_04790"
     CDS             complement(7989..8576)
                     /gene="hisB"
                     /locus_tag="PKW44_04790"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HQR50934.1"
                     /translation="MRTAQITRNTLETKITASINLDGSGKAELASGIGFLDHMLDQIA
                     RHGLLDIGVAAKGDLHIDAHHTVEDIGITLGQAFAKAIGDKKGIRRYGHAYVPLDEAL
                     SRVALDISGRPGLEFGVEFSRARIGDFDVDLIHEFFQGFVNHALVTLHIDNLRGDNAH
                     HQAETIFKAFGRALRVAVEADPRMAGTMPSTKGSL"
BASE COUNT         1900 a         2683 c         2340 g         1691 t
ORIGIN      
        1 ggatgccggt cttggggtcc ttgtgtttct tgatttcgat gccgtcgggg ttattcgacc
       61 ccacttcgtg cagcgcgatg atgtgggcgg ccaccaatcc aagcagcacc agcggcagag
      121 caatcacgtg gaaagcgaag aagcggttga gggtggcatc ggaaatggtg aagtcgccgc
      181 gcagccaaag tgaaatatcc cgcccgatga acggaatgga ggtaaacagg ttgacgatca
      241 cctgcgcacc ccagaacgac atctgccccc aaggcagcag gtagccaaag aaggcctcac
      301 ccatcaggca gaggaaaatg gccacgccga acagccagat cagttcgcgc gggttgcgat
      361 gcgagccata cagcaagccg cggaacatgt gcagatagac cacgatgaag aacatcgagg
      421 cgccggtgga atgcatgtag cggatcagcc agccccagga cacgtcgcgc atgatgtatt
      481 cgaccgaagc gaacgccaga ttggcgtccg gcttgtaatg catggtgagg aaaatgccgg
      541 tgacgatctg gatcaccagc accagcatcg ccagcgagcc gaagtagtac cagaagttaa
      601 agttctttgg cgcatagtat tcagaaaggt gggctttcca gttggcagtc aacgggaagc
      661 gcgcatctac ccagttcagc acgccggtta ggtatttttc catgctcatg ccccctttcc
      721 gtcttcgccg atcaatacgc gggtatcgct cagatatttg tattgcggca cgatcaggtt
      781 ggtcggtgcc ggcgatccct gaaatacgcg gccagcaagg tcgaacttcg aaccatggca
      841 gggacagaaa aagccacccg gccagtcggc gcccatgccc tcctcgcccg gcttgagctt
      901 gggtgatggc gaacagccca aatgcgtgca gatgccaatc accaccatgt attccggctt
      961 gatcgagcgc gttggcgctt tgcaatactc cggctgctgc ggcaccgaac aatccgcatc
     1021 ggtgagcttg tcattatggt tggtcagaag ttccagcatt tccttgctgc gattcacgat
     1081 ccacaccggc ttgccctgcc actccacagt aatcatggcg ccggattcga ttttcgccac
     1141 atccacctcc accggggcgc cggctgcctt ggcgcgctca ctcggcagca tgcttttgac
     1201 gaaaggaaca gccacggcaa ccgtcgccac ccctcccatc gccgccgtcg cggcaaccaa
     1261 aaaccgccgc ttgctctgat ccacttcctg atttgccatc acgatccctt ggtgaatgtg
     1321 atctaaaagg gcgtaattgt aacgatttcc aagggaaact aaaagctgca agcactagac
     1381 cggattggga atttcgatat attgatgacc aataccgaaa cgctccgcaa gatgctctcc
     1441 cagtgcctgc acgccatagc gctcggtcgc atggtggccg gcggcaatga aggccacgcc
     1501 cgattctcgt gccaggtgta cggtatgctc cgacacctca ccagtgagga aagcatccac
     1561 ccccagcgca atcgcctgtg ccagataatc ctgcgctgcg ccgctgcacc aggcgatgcg
     1621 gcggatcgtc gcacttccat ccccgaccag cagtggcgtg cggtcaaatt tttgcgcaac
     1681 tgactgcccc aatgctgcca gacttgcagg ctggctcagc ctgccatgcg cggcaatcgg
     1741 ctgctcaccg aaccagccct cggtttcgaa accaagcaca gcggccagac gggcattatt
     1801 gcccagttct ggatgcgcat ccaacggcag gtggtagcca accaagctga tgtcacgctg
     1861 caacagaaac ttcaaccgat cgcgcttgat ccctgtcacg cgcgcgtcct cgcttttcca
     1921 gaaccagccg tgatgcacca gcaccaggtc ggcacccaat tccgcagcgg cttgcagcag
     1981 cgccagactg gcagaaaccc ctgtgacgat gctgccgacg tcactccggc cctctacctg
     2041 caagccgttg ggacaatagt cgcggaatcg gtcaacctgc agcaattgtc cggtataatc
     2101 aagcagttcc cccaatttca tggcatatac ctcgatttca gcccactcaa catgtgcaaa
     2161 ctctggctca ttttcagttc cggcatatcg catcctgacg cattagcctg ctctgaaatc
     2221 tcgccagaga aatggcttac tctatcatga agaaactctg gctcgttttt gcacaaaccg
     2281 tcaccatcag tctcgccatt ctgttcgtgg tgcagacctt caagccgggt ctgctcggcc
     2341 ttggcgaaag agtcatcgct catcaagtgc agggaacaac gccaacaaca gagactgttt
     2401 cttcatatag cagcgctgcc agaaaagcga tgccttctgt ggtcaacatt tttaccagca
     2461 aggaagccac tgctggcaat cctcagatca tggacgaccc gcagttccga catttctttg
     2521 gtgatcagtt tgacgaccag cctcaacgcg agaacagcct tggctccggc gtcatcgtca
     2581 gcccggatgg actgatcctc accaatcatc acgtgatcga gtccgccgac gaaatcgagg
     2641 tggcacttgc ggacgggcgc accgctcctg cccgcgtggt aggcagcgac cctgaaactg
     2701 atctggcggt agtcaaggta gacctgaaga acttgccggc cgtcactttc ggccagtcag
     2761 acaaacttga cgtcggtgac gtggtactgg caatcggcaa tccgttcggc gtcggacaga
     2821 ccgtcaccca gggcatcgtc agcgcactgg ggcgcaatca tctgggcatc aacaccttcg
     2881 aaaattttat ccagaccgac gcccccatca accccggcaa ttcgggtggc gcactggtcg
     2941 atgccagcgg caacctgatt ggcgtgaaca gcgtcatcta ttcacgcagc ggcggatcaa
     3001 tgggcatagg cttcgccatc ccggtcagcc tggccaggca ggtaatggaa cagatcatcc
     3061 gccaaggcag cgtcacgcgc ggctggatcg gtatcgaggc gcaagacatc tccccggaac
     3121 tggcggaatc gttcaaactc aaggaggcgc gcggcgccct gatcgccggc gtgatgcgcg
     3181 gcggcccggc cgacaaggcc ggcatcaagc cgggcgacgt gttgctggag atcaacggga
     3241 acaaggtgat ggacggctcg tccatgctca acctggtcgc ggcactcaaa ccggaacatc
     3301 ctgccactct gaaaattgcg cgcaaccaga ctgagctcaa tctgccggtc gtcgtcgcca
     3361 agcggcccaa acctctgaca ccgccgcgcg agcagcatta gactatggct ggctcgcttc
     3421 atcgcgcttg atcagtccag caacgaccac gcccgcctcg aacagcaacc ataacggcac
     3481 tgccagcatg acctgcgata ccacgtccgg cggggtgaat atggcaccga ggataaaggc
     3541 gccgacgatg acgtaagaac gggcttcctt aagctgcgca acactgacca agccaaattt
     3601 caccaacgcc accaccacga tcggcacctc gaacgcaatg ccgaaagcga tgaacagcgt
     3661 gatgacgaaa tccaggtagt tgccgatatc agtcatcacc tgcacccctt ccggcgcggt
     3721 ggagacgatg aagccgaata ccaccggaaa caccaggaaa taggcaaatg ccatccccag
     3781 caggaacagc agcgtactgg ccaccaccag cggcgcgata aagcgcttct cgtgcgcata
     3841 cagtccaggc gcgatgaaac tccagacttg atacagcgtg tatggtagcg aaaccactag
     3901 cgctgccatc atcgccacct tcatcggcac cagaaacggc gtggtgacag cagtggcgat
     3961 caattggcct cctgccggca gtttggcgag caaaggctgg gccagggtgt tgtagagtcg
     4021 gttggcaaac gggaacaggc agatgaaggt gatgatgatg ccgaccacca tgcgcaacag
     4081 ccggtcgcgc agctcgatca ggtgggcgat aaaggtttcc gactcgctca tggcgacttg
     4141 gctacatcgt caaccgtcga cttgattgtg cgctcaacat cgccagactc ggcggcaacc
     4201 tggctttcga ccgaggcaat ggtatgcctg gcctcacctt ccagcttgcg cagctcatcc
     4261 agatgcaatt cacggtcgat gtcggccttg accgtagaaa cgtagcgctg catacgtcca
     4321 gtcaacaacc ccagcgtacg cgccaccttg ggcagccttt ccgggccgat gacaaccagc
     4381 gccaccacgg cgatgaccag gatttcggaa aaaccaatgt cgaacaacta gggcgcgccc
     4441 aaactattcg ctcttggact tctgcgtaac ttcgccctcg acggtacggc cgccccggcc
     4501 ttccaccttg ggcgtcgtct gctcttcgcg catggcgtcc ttgaagcctt tgaccgcacc
     4561 gccgagatcg ctgccgatgt tacgcaattt cttggtgccg aatatcagca acaccaccac
     4621 caacacaatc aaccagtgcc agatactgaa agaacccatg tcgtttctcc taaactttgg
     4681 gcagcgtgct gctaccacca aacacatgca catgcaggtg aaacacctcc tgccccccgg
     4741 aacgcccggt gttgatcatg gtacgaaaac catccagccc ctgctccgca gccagcctgg
     4801 gcgcgagcag cagcagtttg cccagtagca cctgatgttg cagttcgcat tccgataagc
     4861 tatctacgtg cgccttgggc accagcaaga aatgcactgg cgcaatagga tggatatcgt
     4921 gaaaaaccag aacctcatca tcctcgtaaa cttttttact cggaattgcg ccgttggcaa
     4981 tcttgcagaa aatacagtcg ctcatgctag ccctcctgac gagacgcttt ctcgtccagg
     5041 cccgacaaac cctcacgccg tgccagctcg ttcaatacgt cactcgggtg caggttatta
     5101 tgcgccagca tcaccaagca gtggaaccac aaatcagcgg tttcatagat aatctgctgg
     5161 cggtcgccac ccttggcggc gatgatggtt tcggcggcct cttcacccac cttcttcagg
     5221 atggcgtcgg aacccttggc atacagcttg gctacgtagg aagactgcgg atcagccgat
     5281 ttgcgttcct ccagcgtccc agccagacga tcaagtatgt cactcatagc gatagatctc
     5341 ttcaggtttt ttgagcacgg gctggtcgac aacccactgc cggcagtcca gtttctggaa
     5401 aaaacaactg tggcggccgg tgtggcaggc aatgccgcca agctgctcca cggttagcag
     5461 gatcacatct tcgtcgcagt ccagtcggat ttccttgacc acctgcacat ggccggactc
     5521 ctcgcctttg cgccacaatt ttttacgtga gcgcgaccaa tacaccgcct ggccagtttc
     5581 cacggtgagc ctgagcgctt cgcggtccat ccaggcgaac atcagcacct gcccgctgcc
     5641 cgcctcctgc gcgatcaccg gcaccagccc ttcgtgcgtc cagttgacct tgttgagcca
     5701 gctatccggc atcatagcct cacttcgatc ccgtggtctg ccatgtactg cttggcttgc
     5761 ctcacggtgt attcgccaaa atggaaaata ctggcagcca gcaccgcatc tgcaccacct
     5821 cgttttaccc catcaaccaa atgatccaga ttgccaacac cgccggaggc gatgatgggc
     5881 acttccactg catcgctgat ggcacgcgtc agctccaggt caaaaccgct ttttgtgcca
     5941 tcccgatcca tgctggtcag cagcagctct cccgctccca accctaccat gcgcttcgcc
     6001 cattcgaccg catcaaaacc ggttggtttg cgcccaccgt gggtgaacac ttcccagtgg
     6061 tcgccgacac gcttggcgtc tatcgccacc acgatgcact gcgagccgaa gcgaccggcg
     6121 gcatccgcca ccagttgcgg gttgagcaca gcagcagtgt taatgctaac cttatcggcg
     6181 ccggcgttga gcaagttgcg cacatcttcc accgcacgaa cgccaccgcc cacagtcagc
     6241 ggaatgaaca cctgcgaagc gacctgctcg atgatatgga aaatcagccc gcggttgtcg
     6301 gagctggcgg tgatgtcgag gaaggtgagc tcgtccgcgc cctgttcatc gtagcggcgg
     6361 gcgatttcca ccggatcgcc ggcgtcgcgc agttcgacga aattgactcc cttgactacc
     6421 cggccgtcgg tgacgtccag gcaaggaatg atgcgcttgg ctaaccccat agaaactctt
     6481 taaccacgga gaacacggag gccactgaga aagaccgctt atcttctccg tgcacggtat
     6541 agttcatgcc gtaatgccgt ttaattcgtc cgccagcgtc tgcgcggcgg cgaaatccag
     6601 cgtgccttcg tagatggcgc ggccggtaat ggcgcccaag atgccttcgt gctccaccgc
     6661 gcacagccgc ttgacgtcat ccaaatcggt gatgccgccg ctggcgatga ccgggatacg
     6721 caagccctgt gccaaggcta cggtagcctc gatattgacg ccgctaagca taccgtcgcg
     6781 cccgatgtcg gtatacacca cggcttccac gccgtaatcc tcgaaacgct tggcgaggtc
     6841 aaccacatcg tggccagtca gcttggacca gccgtccacc gccaccttgc cgtccttggc
     6901 gtcgagaccg accatgatgt ggccgggaaa ggcatcgcac gcctcatgca ggaagccggg
     6961 gttcttcacc gcggcggtgc cgatgatgac gtagctgatg ccgtcgtcca gatagcgctc
     7021 tatggtgtcc aggtcgcgga tgccgccgcc gagctggatg gggatgtcgc cccccaccgc
     7081 atcgacgatg gacttgatgg cgccttcgtt aaccggcttg ccggcgaagg cgccgttgag
     7141 gtcgaccaga tgcaggcggc gtccgccttt atccacccag tggcgcgcca tggcgccggg
     7201 gtcttcggaa aacacggtgg catcttccat gagcccctgc ttgaggcgca cgcaatggcc
     7261 gtctttaaga tcgatcgcag gaattatcag catgatatgg aattgatagt ttagggacgt
     7321 ccgtcccagt taacaaagtt gtataacaag gaaagtcccg cctgctggct tttctcgggg
     7381 tggaattgca cggcgaaaat gttatcgtgc ccgaccgcgc tggtaaagcg cgacggatat
     7441 tcggtatagg cggtttccag cgcgggatcc gtcggctcca cgtaatagct gtggacgaaa
     7501 taatagcgcg catccagcgc gatcccggcc catagtggat ggctacgcgc ctggcgtacc
     7561 tcgttccagc ccatgtgcgg aactttcaac ttgctgccgg cagcatcgtg catgccggaa
     7621 gggaagcgcc tgacccgccc cgggaaaatt ccaaggccga gcgtatcacc ttcttcactg
     7681 tgcgagaaca acatctgcag gccgatgcaa atgccaagga aaggcttgtt acgcgccgca
     7741 accagcagtg gttggcgcag gccacgtgca tccagttcgc gcacgcagtc cggcatcgcg
     7801 ccttggccgg gaaacacgac cctgtccgca gccagcactt cttccggatc gctggtgacc
     7861 agcacggact ggtcccccgc cacgtggcgc aaagcctgct ctacggaacg caaattgccc
     7921 ataccgtaat cgatgactgc aatgtctgcc atagttggac ggcggttatg acgcctggta
     7981 aggaaaaatt aaaggctgcc cttggtggac ggcattgtcc cggccatgcg cggatcggct
     8041 tccaccgcca cgcgcaatgc ccggccaaaa gccttgaaga tggtttctgc ctgatgatgt
     8101 gcgttatcgc cgcgcaaatt gtcgatgtgc agcgttacca gggcatggtt gacgaagccc
     8161 tggaaaaact catgaatgag atcaacatcg aaatcgccga tacgggcccg gctgaattcg
     8221 accccgaact ccagtccggg acggccggag atgtccagtg ccacgcgcga caaggcttca
     8281 tccagcggca cataggcatg gccgtaacga cgaatgcctt tcttgtcgcc gatggccttg
     8341 gcgaacgcct gccccagggt gatgccgatg tcttccacgg tgtgatgcgc atcaatatgc
     8401 aggtcgccct tcgcggccac gccgatatcc agcaaaccat ggcgggcaat ttgatccagc
     8461 atgtgatcga ggaacccaat accactcgcc agttcagcct taccgctgcc gtccagattg
     8521 atggaggcag tgatcttggt ttccagagta ttgcgtgtga tttgtgcggt gcgcatggat
     8581 tgatcactga aaaggtggcc tattctaccg ccca
//
LOCUS       DAOMFA010000067         8505 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_956_length_8505_cov_5.140710, whole genome shotgun sequence.
ACCESSION   DAOMFA010000067 DAOMFA010000000
VERSION     DAOMFA010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 8505)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8505)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8505
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_956_length_8505_cov_5.140710"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..731
                     /locus_tag="PKW44_04795"
     CDS             <1..731
                     /locus_tag="PKW44_04795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR50935.1"
                     /translation="GSADFNGGKTYWIRTGAGAEWRSGSWQMSGEVNAGEQEDPGILA
                     TLRWQPDDYWKFDVHGESKTNDIPLQAVNAGVTASRIGLGIDWRAHEARKLAVEGNVL
                     NFTDGNLRKTVIGTWFERWLSGPRWSFETTIGADASNNSIGYRADYFNPPNDHSYWVT
                     GAVEQLAWRDYNYSFRQRLALSLGSYWQSGYGAGSTESLEYQHSWELGRAAWLRYSIG
                     RTLRPYDGEREQRTFGTMTLLWRF"
     gene            719..2287
                     /gene="pgaB"
                     /locus_tag="PKW44_04800"
     CDS             719..2287
                     /gene="pgaB"
                     /locus_tag="PKW44_04800"
                     /inference="COORDINATES: protein motif:HMM:TIGR03938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     N-deacetylase PgaB"
                     /protein_id="HQR50936.1"
                     /translation="MAILNGIRILQFAVLMMACSCFASTAAPGDFVVLCYHEVESDRT
                     PTLTGTAVHASDLAAQFAWLKENGYHPVSLQQILDSRQNGPALPDKAVLLTFDDGKKD
                     VYTRVFPLLKLFHYPAVVAVVGRWLDVPEGGLADYDGKLLPRSEFVTWDEIREMQRSG
                     LVEIASHSYDLHRGVIANPQGNTQPATTTRLYSEGNYETDTVYVDRLESDMRRNRDLI
                     ERETGHAPRAMVWPYGRTNLVAQQLSTKLGMPVTMTLDDGVNTVSTPLSAMKRYLIEQ
                     SPSLQSFAEAMRKVWSADPARSVRIHPGEWPVLEKDLSLTLDRLQRLSVNIVFVDPET
                     KSGPQPSPLFPSERHPSAADELNHIAWQIERRAGSPVFIDIPAAWLKDLDLLSELARQ
                     VNFAGVRMPVLPGDEAARRALAAMERWCWPLRVAYAPEGTVPASAWQMLLPGDLVVMP
                     ATKENLVAVPVTEAGNVLFEFDPTSQPASEIAREMRRLEADGFRQFGLADFPQAGYDA
                     VWKAISLRSQPLLP"
     gene            2284..3573
                     /gene="pgaC"
                     /locus_tag="PKW44_04805"
     CDS             2284..3573
                     /gene="pgaC"
                     /locus_tag="PKW44_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013146753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008375 - acetylglucosaminyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042710 - biofilm formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine synthase"
                     /protein_id="HQR50937.1"
                     /translation="MTHPDLTLPALLGNFWGIMLSYVFLYPVLMSCLWMIGGLAFYFR
                     HERHPHRIVNQPPERDEYPLVAILVPCFNEEANARETIDALMALRYPNFEVIAINDGS
                     KDRTGEMLDALADEYPRLRVIHNIKNQGKAVGLNTAALLTHAEILVGIDGDARLDPWC
                     LHWLVRHFDVPEVGAVTGNPRVRTRSTLLGRIQVGEFSSIVGLIKRAQRSIGLIFTVS
                     GVIAAFRRSALFEAGFWSPDKMTEDVDISWKLQLADWEVRFEPRALCWILMSETLKGL
                     WNQRLRWATGGAQVIFDYWLEIWHRPRMWPVFVEYSMSIVWAFCMALLLLYRIADLVI
                     YKVDLQSVPVLMLGWTGILIGMICMVQMLLSLWLDRPYDQGLLKNYFWMIWYPIIYWL
                     LSAATAVAALPAALTRKSGKRATWISPDRGLQQNNDL"
     gene            3528..4001
                     /gene="pgaD"
                     /locus_tag="PKW44_04810"
     CDS             3528..4001
                     /gene="pgaD"
                     /locus_tag="PKW44_04810"
                     /inference="COORDINATES: protein motif:HMM:TIGR03940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008375 - acetylglucosaminyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0042710 - biofilm formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     biosynthesis protein PgaD"
                     /protein_id="HQR50938.1"
                     /translation="MDQSGPRVAAEQRPLIIERPDLAHPVRRVLAFVLTLLAWTGFVA
                     LWLPALDVAAAALGLPDVPWVFTGTRNLMALDELFDVFPLAIGLMLLVLATNGLISRI
                     YRRFRKPVHHRFVGMEQLATGMALDQNKLAAWQAARILHVEHGPLGRVINANVIR"
     gene            4267..6930
                     /locus_tag="PKW44_04815"
     CDS             4267..6930
                     /locus_tag="PKW44_04815"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HQR50939.1"
                     /translation="MNNIWLLALFLFASETALMFFLDHVKIVEHGGTLHNLLDGLLLV
                     AIMLPIFYKLSRGRSTLEGEKRRLTENEERLRQLGDNLPGGFIYQIMQEADGSPRFIY
                     LSAGVVPLTGVTIDEAMKDANAIRGLLDPAQAPRILAIEAACVRDLSIFEAELRMRRR
                     DGEWRWLQFRSRPRRLDQGKIIWDGVAFDITERKLVEDKLHLAARVFSEAHEGIIITD
                     TAGVIVDVNPTFCNITGYSRDEVIGKNPNLLNSGKQTAEFFASLWDALNRGGYWQGEL
                     WNRKKNGELFAERLTISVLRDEQGAVVHYLGLFSDITQTKQQQQTLELMAHYDVLTRL
                     PNRVLFADRFAQAIAHGRREETLLAVCYLDLDGFKHINDTLGHEAGDRLLVEVANRIK
                     ATLRQEDTVSRLGGDEFALLLGDIGTLDQGEQALARIIQAVAQPHLLDGQEVSISASI
                     GVTIFPLDDADPDTLLRHADQAMYQAKLDGRNRYHLFDSSHDRQAKTRQHLLDEIRQA
                     FARNEFCLYYQPMVNMKTGAVLGVEALLRWQHPERGVILPGQFLPLLEGSAMEITFGN
                     WVIEQALRQLGYWNSRGLKLEVSVNISPNHLQWSGFLAQIEAALAQHPGIASSQLELE
                     VLESSVPGDLAAASEVIRNCRNALGVSVALDDFGTGYSSLTQLRRLPTNTIKIDQSFI
                     SDMIDDPNDFAIVGGVIGLAGAFRRDVVAEGVESMEHGLILLTMGCVYGQGNGIARPM
                     PADGLMSWVQSYQPYPEWKNYAVNRLDRGAEQLLLAKIESDQWLRRLEECLQSDPGSP
                     AHWPIMDHRKCHCGRWVNQAKQEQLFDATLLDRLDVAHEELHRVGNALMREFQAGNVD
                     KARVGIEDLRAVYRKVCQILEQCGATELLKP"
     gene            complement(6948..7418)
                     /locus_tag="PKW44_04820"
     CDS             complement(6948..7418)
                     /locus_tag="PKW44_04820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR50940.1"
                     /translation="MTQLEHGFSALSAADRQLIEDEHARLELFLQDLRDTCCEYETTQ
                     SCQGCGKEKAACCQGRLTSFFYDFLDLIEQHFENEENIMRRLTPAAEDGLYFIQHQQA
                     HAGLMLKVRAAMHEAGALNKQGKTAEAIRLLYRQVPAMFGEHACRFDDAFLRAA"
     gene            7515..8030
                     /gene="greB"
                     /locus_tag="PKW44_04825"
     CDS             7515..8030
                     /gene="greB"
                     /locus_tag="PKW44_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HQR50941.1"
                     /translation="MAAEYKNYITPEGYTRLKDEFDQLYRVERPRVVADVAWAASNGD
                     RSENADYIYGKRRLRQIDSRLKFLMKRMDLAEVVDPARQQGLDKVFFGAWVRLYSITH
                     DSEHTYRIVGQDELDLSRGYISWISPLARALMKKGEGDSVKLETPGGEEEYEVLDISY
                     STPPLSSAHQA"
     gene            complement(8061..>8505)
                     /locus_tag="PKW44_04830"
     CDS             complement(8061..>8505)
                     /locus_tag="PKW44_04830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR50942.1"
                     /translation="AAVVWLLWAKMPPQSMSMPVSAAPSTSLSATHKEEAIAKPEAIP
                     PASPAPIHPDRVGAKPAGKESKILEQEKQAQDSGSDKPEREASSETHTQPAGSEPQKP
                     EQEKQVQGSGLEKLGLDKLKQLKQYLPQGQKAPCGDSERALGQCQ"
BASE COUNT         1741 a         2591 c         2472 g         1701 t
ORIGIN      
        1 gcggcagcgc agatttcaat ggcggcaaga catactggat tcgcaccggc gccggtgcgg
       61 aatggcgttc cggaagctgg cagatgagcg gggaagtcaa tgcaggcgaa caggaagacc
      121 ctggcatact ggcgaccctg cgctggcagc ctgatgatta ctggaagttt gatgtacatg
      181 gcgaaagcaa gactaacgat atcccgctac aggcggtgaa tgccggggtc accgccagcc
      241 gcatcggact gggcatcgac tggcgggcac acgaggcgcg gaaactcgcc gtggaaggca
      301 atgtgctgaa cttcaccgac ggcaacctgc gtaaaacagt catcggcact tggttcgagc
      361 gctggctcag cggcccccgt tggagcttcg agaccactat cggcgcggac gcctcgaaca
      421 attcgatcgg ctaccgggcc gactatttca accctccgaa cgaccactca tactgggtga
      481 cgggggccgt ggaacagctc gcctggcgtg actacaacta ttcgttcaga cagcgtctgg
      541 ccctatcgct gggcagctac tggcaatccg gctatggcgc cggcagcacc gaatcgctcg
      601 aataccagca cagctgggaa cttggacgtg ccgcatggct gcgctattct atcggccgaa
      661 ccctgcggcc ttacgacggg gagcgcgaac agcgcacttt tgggacaatg accttgctat
      721 ggcgattctg aacggcatcc ggatactgca gtttgccgtc ctgatgatgg catgttcgtg
      781 cttcgcgtcg acagctgctc cgggcgactt cgtcgtactt tgctaccacg aggtggaaag
      841 cgacaggacg ccaaccctga ctggcaccgc tgtccacgcg agcgacttgg ctgcacagtt
      901 cgcatggctg aaggaaaacg gctatcaccc ggtcagcctg caacaaatac ttgactcccg
      961 gcagaacggc cctgccctgc cagacaaagc agtactgctg accttcgatg acggcaaaaa
     1021 ggatgtctac actcgcgtct tcccgctgct caagctattc cactatcctg ccgtagtcgc
     1081 tgtggtcggc cgctggctcg acgtgcctga aggaggcttg gcggattacg atgggaaact
     1141 cttgccacgc agcgaattcg tcacttggga tgaaattcgc gaaatgcagc gctcggggct
     1201 ggtcgagatc gcctcgcatt cttatgacct gcaccgcggc gtcattgcca atccacaggg
     1261 aaatacccag ccggccacga caacgcgtct ctactccgaa ggaaattacg aaaccgatac
     1321 ggtttatgtc gatcgcctgg agagtgacat gcgccgcaac cgtgacctga ttgaacggga
     1381 gaccgggcat gcgccgcgcg ccatggtctg gccttatggt cgcaccaacc tggtagcgca
     1441 acaactttcc acaaagctgg gtatgccagt aaccatgacg ctcgatgacg gcgtcaatac
     1501 cgtgtctacg ccgttatcgg cgatgaaacg ctatctgatc gaacaaagcc catcgctgca
     1561 aagctttgcc gaggccatgc gaaaagtctg gtctgcagac cctgcacgca gcgtaagaat
     1621 ccacccgggg gaatggcccg tgctggagaa agatctgtcg ctgacgctgg accggctgca
     1681 gcgattgagc gtgaacatcg tctttgtcga ccccgagaca aaaagtggcc cccagccatc
     1741 ccccctgttc ccatccgagc gccaccccag cgccgccgac gaattgaacc acattgcatg
     1801 gcagatcgag cgtcgtgccg gaagccctgt gtttatcgac ataccggctg cctggctgaa
     1861 ggatctggac ttgctcagcg agcttgcccg ccaagtcaat tttgccggcg tccggatgcc
     1921 tgtgctgccg ggcgatgagg ccgccagacg cgcgctggcg gcaatggagc gctggtgctg
     1981 gccgctacgc gtcgcttatg cgccggaagg caccgttcct gccagtgcgt ggcagatgct
     2041 tctcccgggc gatctggtgg tcatgcccgc caccaaggaa aatttggtcg ctgtcccggt
     2101 aactgaggca gggaacgtac tcttcgaatt cgatccgact tcgcagcccg cctctgaaat
     2161 tgcccgggaa atgcgccgac tcgaagccga cggattccgc cagttcggcc tcgccgactt
     2221 cccgcaagcg ggttacgacg ccgtctggaa agccatctcg ctgcgttcgc aaccgctcct
     2281 gccatgacgc atcctgacct gacgcttccc gcacttctag gcaacttctg gggcatcatg
     2341 ctctcgtacg tattcctcta cccggtgctg atgtcgtgcc tgtggatgat cggcgggctg
     2401 gcattctatt ttcgtcacga acggcatccg caccgcattg tcaaccaacc gccggaacgg
     2461 gatgaatacc cactggtcgc gattctagtt ccttgtttca acgaggaagc gaatgcgcgc
     2521 gaaaccatcg atgcgctgat ggcgctgcgc taccccaatt ttgaagtcat cgccatcaac
     2581 gatggcagca aggaccgcac aggggaaatg ctggatgcgc tggccgacga atacccacgc
     2641 ctgcgcgtga ttcacaatat caagaaccag ggcaaggcag tcgggctgaa caccgcagca
     2701 ctactgacgc acgccgaaat tcttgtcggc atcgacggcg atgcacgcct cgatccctgg
     2761 tgtctgcatt ggctggtgcg tcattttgat gtaccggaag tcggcgcggt cacgggcaac
     2821 ccacgggtac gaacgcgctc gacactgcta ggacgcattc aggtcggtga gttctcgtcc
     2881 atcgtcggcc tgatcaaacg ggcgcagcgc tcaatcggtc tgattttcac cgtttccggc
     2941 gtaatcgccg cattccgccg cagcgccctg ttcgaagccg gcttctggag cccggacaaa
     3001 atgacggaag acgtcgatat cagctggaag ctgcaactgg ccgattggga agtgcgtttt
     3061 gagcctcgcg ccctgtgctg gatcctgatg tcggagacgc tcaagggctt gtggaatcag
     3121 cgtttgcgct gggcgaccgg cggcgcgcag gtcattttcg attactggct ggaaatctgg
     3181 caccggcccc gcatgtggcc agtgttcgtc gaatattcga tgagcatcgt ctgggcattc
     3241 tgcatggcct tgctgctgct ctaccgcatt gccgacctgg tgatttacaa ggttgacctg
     3301 cagagcgtgc cggtactcat gctgggctgg acgggcattc tcatcggcat gatctgcatg
     3361 gtgcagatgc tgctcagcct gtggctcgac cgcccctacg atcaaggatt gctgaaaaat
     3421 tacttctgga tgatctggta tccgattatc tactggttat tgagcgctgc taccgctgta
     3481 gcagcattgc cggcagcact cacgcgcaaa tcaggaaaga gggcaacatg gatcagtccg
     3541 gaccgcgggt tgcagcagaa caacgacctc tgatcatcga gcgccccgat ctcgcgcacc
     3601 cggtgcggag ggtgcttgcc ttcgtcctga cactgctggc ctggacaggt ttcgtcgcac
     3661 tgtggctgcc ggcacttgac gtggccgcag cagccctcgg cctgcccgac gtgccttggg
     3721 ttttcacggg cacacgcaac ctcatggcgc tggatgaact ctttgatgta tttcccctgg
     3781 ccatcggact gatgctgctg gtactcgcca ccaacgggct gatcagccgg atctatcggc
     3841 gtttcagaaa accggtgcat caccggtttg tcggcatgga acaactggct acagggatgg
     3901 cgcttgacca gaacaaactt gcagcttggc aggccgcccg tatcctccac gtcgagcatg
     3961 ggccattggg gcgtgtcatc aatgccaacg ttatccgttg aggctacgac ggcaagcacc
     4021 tttccgcacc tttccacacc ttgtcggcat cagcgcaccg gccatcgaca aaccatcaaa
     4081 atcacctgat ttcgtgttgt ccggacgcca gttttgtata cactgtcagc aacggccttt
     4141 tctttcattc gcagacatgc ctatcagacc catggtgaac gcctgcctct ccctgccggc
     4201 cgcgcagctg caggtagccg tcgtgcgcgc ctttccttgc ttgtcgtgcc gcagcattct
     4261 ctcctcatga acaacatctg gctgctggcg ttattcctgt tcgcaagcga aaccgcgctg
     4321 atgtttttcc ttgatcacgt caagatcgtg gaacacggcg gcacgctgca caacctgctc
     4381 gatggattgc tgctggttgc catcatgctg ccgatcttct acaagctgtc gcgcggcaga
     4441 agcaccctgg aaggggaaaa acggcgcctg acggaaaacg aggaacggtt gcgtcaactc
     4501 ggcgacaacc tgcccggcgg ctttatttac cagatcatgc aggaagctga cggcagcccg
     4561 cgttttatct atctcagcgc cggggtggtg ccgttgaccg gcgtcactat cgatgaggcg
     4621 atgaaggacg ccaatgccat acgcggcctg ctggatccgg cacaggcgcc gcgcatcctc
     4681 gcgatcgaag ccgcatgtgt acgcgatctc agcattttcg aagccgaatt gcgcatgcgg
     4741 cggcgcgatg gcgaatggcg ctggcttcaa ttcagatcac gcccgcgccg cctcgaccag
     4801 ggaaagatta tctgggatgg cgtcgctttc gacatcaccg aacgcaaact ggtggaggac
     4861 aagctgcacc ttgccgcccg cgttttcagc gaggcgcatg aaggcatcat catcaccgac
     4921 actgccggcg tgatcgtcga cgtcaacccg acgttttgca acatcaccgg ctacagccgt
     4981 gacgaagtca tcggaaaaaa tcccaacctg ctcaattcgg gcaagcagac tgccgaattt
     5041 ttcgccagcc tgtgggacgc gctgaaccgt ggcggctact ggcaaggcga actatggaac
     5101 cgcaagaaga acggggaatt gtttgccgaa cggctcacca tttccgtatt gcgcgacgag
     5161 caaggcgccg tcgtacatta cctcggactg ttttccgaca tcacgcagac caagcagcag
     5221 cagcagacgc tggaactgat ggcgcactac gacgtactga cccggctacc caaccgggtg
     5281 ttgttcgccg accgcttcgc ccaggcaatc gcccacggcc ggcgggaaga gaccttgctg
     5341 gccgtatgct acctcgatct cgacggcttc aagcatatca atgacacgct gggccacgaa
     5401 gcgggtgacc gccttctggt cgaggtggcg aaccgcatca aggctaccct gcggcaggaa
     5461 gacacggttt cgcgcctggg cggggacgag ttcgccctgt tgctggggga catcggcacc
     5521 ctagaccagg gcgagcaggc gctggcacgc atcatccagg cggtagcgca accccacctg
     5581 ctggatgggc aggaggtttc catttccgcc agcatcggcg tcaccatttt cccgctggac
     5641 gatgccgacc cggacaccct gctacgccac gccgaccagg ccatgtatca agccaaactc
     5701 gacgggcgca accgttacca cctgttcgac agttcgcacg acaggcaggc taaaacccgc
     5761 cagcacctgc tggacgagat taggcaggcc ttcgctcgca acgagttctg cctgtattac
     5821 cagcccatgg tcaacatgaa aacgggcgcc gttctgggcg tcgaggctct gctacgctgg
     5881 caacaccctg aacgcggcgt cattctgcca gggcaattcc tgccgctgct cgaaggctcg
     5941 gccatggaga tcaccttcgg caactgggtg atcgagcagg cattgcgcca gctgggatac
     6001 tggaactccc gggggctgaa gctggaagtc agcgtcaaca tttcgcccaa tcacctgcaa
     6061 tggagcggct ttctcgccca gatcgaggca gcgctggcac agcacccggg catcgcttcc
     6121 agccagctgg agctcgaagt tctggaaagc agcgtacctg gcgatttggc tgctgccagc
     6181 gaagtcatca gaaactgtcg caacgcgctc ggcgtcagcg tggcgctgga tgatttcggc
     6241 accggttatt cgtcgctgac ccagttgcgg cggctgccta ccaataccat caagatcgac
     6301 cagagcttca tctccgacat gattgacgat cccaacgact tcgccatcgt cggcggggtg
     6361 atcggcctcg ccggcgcttt ccggcgcgat gtcgtcgcag aaggagtgga gtctatggag
     6421 cacggcctca tcctgctgac catgggctgc gtttacggcc agggcaacgg cattgcccgc
     6481 cccatgccgg ccgacgggct gatgagttgg gtccagagct accagccgta cccggagtgg
     6541 aaaaattatg ccgtcaatcg gctcgaccgc ggtgccgaac aactactgct ggcgaagata
     6601 gagtcagatc agtggctgcg acgattggaa gaatgcctgc aatcggatcc cggctcgccg
     6661 gcgcactggc ccatcatgga tcacaggaaa tgccattgcg gccgctgggt caatcaggcc
     6721 aagcaggagc agttattcga tgcgacgctg ctggatcggc tggatgtggc tcacgaggaa
     6781 ttgcaccgtg tcggcaacgc cctcatgcgc gagtttcagg ccggaaacgt cgacaaggca
     6841 cgcgtgggaa tcgaggacct gcgcgcagtc taccgcaagg tctgccagat tctggaacaa
     6901 tgcggggcta cagaattgct taagccctga aaccgcttca ggcttgctca ggcagcacgc
     6961 aagaaagcat catcgaagcg gcacgcgtgc tcgccgaaca tggccggaac ttgccggtag
     7021 agcagccgga tcgcttccgc cgtcttgcct tgtttgttca gcgccccggc ctcgtgcatc
     7081 gccgcccgca ccttcaacat gagtccggca tgcgcttgct ggtgctggat gaaataaagc
     7141 ccgtcttccg cggcgggtgt cagcctgcgc atgatgtttt cttcgttttc gaaatgctgc
     7201 tcgatcagat cgagaaaatc ataaaaaaaa gaagtcaatc gcccttggca gcaggcggct
     7261 ttctcttttc cacaaccttg acacgactgc gtcgtctcgt actcgcaaca ggtgtcgcgc
     7321 agatcctgca ggaacaattc cagacgagcg tgctcatcct cgatcaactg acgatctgcg
     7381 gccgaaagcg cgctgaaacc atgctccagt tgcgtcatga attctcccgg ccgtgaagga
     7441 atcggagagc gtatcctacg ccgctgatgt tcggagtcaa taggacggaa aattcgcgct
     7501 gtataatccg ccacatggca gcagagtata aaaactacat cacgcccgaa ggctacacgc
     7561 ggctcaagga cgagttcgat cagctttacc gggtggagcg ccccagggtg gtcgccgatg
     7621 tcgcctgggc cgccagcaac ggggaccgca gcgaaaacgc cgactacatt tatggcaaac
     7681 gccgtctgcg ccagatcgac tcgcgcctca agttcctgat gaagcgcatg gatctggcgg
     7741 aagtcgtcga ccccgcgcgg cagcagggcc tggacaaggt cttttttggt gcctgggtca
     7801 ggctctattc cattacccac gacagcgagc acacttaccg catcgtcggc caggacgaac
     7861 tcgacctgtc caggggctat atcagctgga tctcccccct ggccagggcg ctgatgaaaa
     7921 aaggcgaagg cgacagcgta aaactggaaa cgcctggggg cgaagaggag tacgaggtac
     7981 tggacatcag ctacagcact ccgccgcttt catccgcgca tcaagcatga cacgacaaag
     8041 cgacggcgac ctcgcaggaa ttactggcat tggccaagcg cacgctcgga gtcaccgcag
     8101 ggcgccttct gtccttgcgg aaggtactgc ttcaactgct tcaacttatc caagccaagt
     8161 ttttccagac cacttccctg cacctgtttt tcctgttccg gcttttgcgg ctcgctgccg
     8221 gcaggctgtg tatgcgtttc cgaggacgct tcccgttcag gtttatctga accgctgtcc
     8281 tgcgcctgtt tttcctgctc caatattttc gactccttgc cggcaggctt ggccccaacc
     8341 cggtctggat gtatgggggc gggtgatgcg ggcgggatgg cttcgggctt ggctatcgct
     8401 tcctctttgt gcgtagcgga cagcgaggta gaaggcgcgg ccgacactgg catgctcatc
     8461 gactgtggcg gcatcttcgc ccacagcaac cagacaacgg cagct
//
LOCUS       DAOMFA010000068         8427 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_976_length_8427_cov_5.077520, whole genome shotgun sequence.
ACCESSION   DAOMFA010000068 DAOMFA010000000
VERSION     DAOMFA010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 8427)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8427)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8427
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_976_length_8427_cov_5.077520"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..315
                     /locus_tag="PKW44_04835"
     CDS             <1..315
                     /locus_tag="PKW44_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HQR50943.1"
                     /translation="LVATENGYGKRTVLADFRHSGRGTQGVIAIATSARNGKVVAAKL
                     VNDDDEIMLITTGGVLIRTRVKEIRELGRATQGVTLINLGEGEKLSGLEKVVETDDEE
                     EA"
     gene            316..1413
                     /gene="serC"
                     /locus_tag="PKW44_04840"
     CDS             316..1413
                     /gene="serC"
                     /locus_tag="PKW44_04840"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HQR50944.1"
                     /translation="MSRVWNFSAGPAVLPQEVLEQVRDEMLEWHDAGMSVMEMSHRGK
                     AFTGIIEQAEADLRALLNVPANYKILFMQGGAIAENAIIPLNLLGDKTAADYVVTGSW
                     SEKSHQEARRYCNANLAASSGPDYTCVPKPGSWQLSQDPAYLFVCTNETIGGVEYAFV
                     PDMTALGKPDVPVAADMSSHILSRPVEVSRYGLIFGGAQKNIGPAGLTIVIVRDDLLG
                     RTSPICPSAFNYKTVADNGSMYNTPPTFGIYVAGLVFQWLKRQGGLAAIEQANLRKAR
                     LLYGAIDGSGGFYHNPIEVESRSRMNVPFTMQDDALDAVFLKQAEAAGLHSLKGHKSV
                     GGLRASIYNAMPLQGVQALVDFMQEFARTHG"
     gene            1416..2483
                     /gene="pheA"
                     /locus_tag="PKW44_04845"
     CDS             1416..2483
                     /gene="pheA"
                     /locus_tag="PKW44_04845"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HQR50945.1"
                     /translation="MSDELKKCREQIDAIDDQLLKLISERAGLAHAIGSLKNGGPIYR
                     PEREAQVLRRLLEQNPGPLSDEAVTAIFRTVMSNCRALEKELAVAFLGPLGTYSEEAA
                     NKQFGGLSSPVQCATIDEVFRTVETGHADYGVVPVENSTEGAIGRTLDLLLQTDLQVC
                     GEIDLPVHHNLLSGAADKSSVRIVYSHAQSLAQCHEWLNANLPGVERQAVSSNSEASR
                     LAAENPASAAIASKRAGELFGLNLLAENIEDDPRNTTRFLVVADHDVAPSGRDRTSLA
                     MSTRNVPGAIADLLAPLAANGISMSKLESRPSHIGMWEYVFFVDIEGHRSDPKVVAAL
                     EMLRDKAAFLKVLGSYPVAVV"
     gene            complement(2566..3378)
                     /locus_tag="PKW44_04850"
     CDS             complement(2566..3378)
                     /locus_tag="PKW44_04850"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004177368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HQR50946.1"
                     /translation="MPKRSVFFISDWTGITAETLGMSLLAHFDGVEFRQVRLPFIDSP
                     DKAQDALARIAAAQAEDGTRPILVLTLVNPKIRQMFEDSGALCLDLFGTFIQPLSVEF
                     GQLPTQTVGMSHRAVGSEYLNRIDAINYTLSHDDGLTPASLEKADVVLVGVSRSGKTP
                     TCLYLAMQFGVRAANCPLIPEDLERMVLPGDLASYRSKLFGLTIDPERLHRIRGERRP
                     DSHYASLNNCRNEVRQAETLMRREGIPWLDSTTRSVEEISTMILQQLKLKGR"
     gene            3407..6124
                     /gene="glnE"
                     /locus_tag="PKW44_04855"
     CDS             3407..6124
                     /gene="glnE"
                     /locus_tag="PKW44_04855"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HQR50947.1"
                     /translation="MITPSAFPKTAGSDEAIIARSRELSPFFHRTLEADALLLPALQS
                     GLHQVFDAEQMRATLAEGAGDDEDALKRKLRRLRKQVMLHLIARDLNGLADLAEVVET
                     CTVLAEVTVISALKHLSHWQRQLYGDPVGESGTVQQLLIAGMGKLGGRELNVSSDIDL
                     IFAYEEDGETNGARQISNHEYFARLGKKLIAAIGETTADGFVFRVDMRLRPYGDSGPL
                     VGSFAALEEYYQTQGREWERYAWIKGRVIAGPEQALQDLLRPFVFRKYLDYGAFASMR
                     DLKAQIMREVARRDMHDNIKLGPGGIREIEFIAQVFQLIRGGQDRELQIRPTLQVLQL
                     LEKKSLLPPETVAELSAAYVFLRNLEHRLQYLQDAQTQMLPADDENRARIAAGMGFAD
                     WAGFLQELGRHRASVERHFSQVFDEPEGEQTHSLAGLWEGSSSEEGLQQQLAALGYRN
                     TAEAWQRLNGLRSASRYKQLPAASRQRFDALVPRALEASARHSNADETLARMLTLLEA
                     ICRRASYLALLAEYPQALDAVARLCSASPWLAAYLTQHPILLDELLDTRSLYAAPDFS
                     ALRDDLESRLADCGEDVERKLDVLRHFKHAQTFRFAAQDLTGELPLETLSDDLSALAD
                     LVLDAVIRHAWPGLRNRHREAPAFSIIGYGKLGSKELGYASDLDIVFLYDDVADNAGE
                     VYARFGQRINNWLNSLTPAGLLYETDLRLRPDGASGLLVSSVEAFTEYQKNKAWTWEH
                     QALTRARFCAGAPAIGNAFEQVRLDILRQPRDLAKLREEVVAMRQKIHDGHPNNTPLF
                     DIKHDAGGIVDVEFIVQTLVLAYARQYPQLAENVGNLALLGRAGEVGLILPDLARSAA
                     DAYRDYRNAQHVQRLQGAEKSRVDREPFVRAIAAVKTLWDQVLGDGLSK"
     gene            complement(6172..7236)
                     /locus_tag="PKW44_04860"
     CDS             complement(6172..7236)
                     /locus_tag="PKW44_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018410189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR50948.1"
                     /translation="MKFHPFAKRLPITLFLALLGITNVLADSKGVAYVSNQDGGVTVI
                     NLATMETTGTLDIGAKGPRGIGITADGKWLVTANKDDGNISIIDTASGKVVKHIPIGK
                     NPEFVRVYGDRAYVTYEPSSKGGPPPKPGAEPAKDNDDEDDLPGHIAIVDLKKGKVVK
                     DITGKPETEGVEFSLDGKRMIVTNESDNSITVHHMKSGKLLKSIPVGSYGERPRGIKT
                     SPDGKFYVSTLEMSDKLLILDNTLNPVKSIDTGKSPYGVSFDRDGKRLYVASSKEKAL
                     QVYDAGTFEKIKDIPTGDRCWHFSFTPDDQNILLACGKSNEVVVIDTQKLEVTKRIGD
                     KDMPWGIVTHPKSMGSLDRP"
     gene            complement(7501..8262)
                     /locus_tag="PKW44_04865"
     CDS             complement(7501..8262)
                     /locus_tag="PKW44_04865"
                     /inference="COORDINATES: protein motif:HMM:NF025389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4239 domain-containing protein"
                     /protein_id="HQR50949.1"
                     /translation="MSSWSVFIVTLICIIAGIVLGAILRDRLPDHHLREDSRDAIKLA
                     SGLIATLVALVLGLLVGSTKSNFDEINNQLVQSGAKIIALDRALVRYGPETSEIRAQL
                     REGLVGAIQRIWPEDGLEKKGTESSTMAQSLGKMADGLGALTPASDQQRQFQPQLQQL
                     VSDLLQSSLLLTEESQSSVPPVFLLVLILWLGILFMSFSLLSQHNLTSTLALIISGAS
                     ISAALFLVIEMSHPFEGIIKVSDAPLLKALAIMAK"
BASE COUNT         1686 a         2404 c         2600 g         1737 t
ORIGIN      
        1 ctggtcgcca ccgaaaacgg ttacggcaag cgcaccgtgc tggccgactt ccgtcactcc
       61 ggtcgcggca cgcagggtgt gattgccatt gccacctcgg cacgcaatgg caaggtcgtg
      121 gccgccaagc tggtcaacga cgacgacgag atcatgctca tcaccaccgg cggcgtactg
      181 atccgtaccc gcgtcaagga aatccgcgaa ctcggccgtg ccacgcaggg cgtgacgctg
      241 atcaacctgg gagagggcga aaaactttcc ggtctggaaa aagtggtcga gactgacgac
      301 gaggaagaag cgtaaatgtc acgcgtgtgg aacttcagcg ccgggccggc ggttctgccg
      361 caggaggttt tggagcaagt ccgcgacgaa atgctggagt ggcatgacgc cggcatgtct
      421 gtgatggaaa tgagccaccg tggcaaggct ttcaccggca tcatcgagca agccgaggcc
      481 gacctgcgcg cgttgctgaa tgtcccggcc aattacaaaa tcctgttcat gcagggcggc
      541 gccatcgccg agaacgctat catcccgctt aacctgctgg gagataaaac agcggctgat
      601 tatgtggtga ccggttcctg gtcggagaag tcgcaccagg aagcgcgccg ctattgcaac
      661 gccaacctag cagccagttc cggacccgac tatacctgcg ttcccaaacc tggaagctgg
      721 caattgtcgc aagacccggc ctatttattt gtctgcacca acgagaccat aggcggcgtg
      781 gaatacgcct tcgtgccaga catgactgcg ctgggcaagc ctgatgtgcc ggtggcggcg
      841 gacatgtctt cgcatatcct gtcacggccg gtggaggtgt cccggtacgg cctgattttc
      901 ggcggtgcgc agaaaaacat cgggcctgct ggactgacga tcgtcatcgt gcgtgatgac
      961 ctgctgggtc gcacctcgcc gatctgcccc tcggccttca actacaagac cgtggctgac
     1021 aacggctcca tgtacaacac gccgcccacc tttggcattt atgtggccgg gctggtgttc
     1081 cagtggctga aaaggcaggg cgggctggcc gccatcgaac aggccaacct gcgcaaagca
     1141 cgtttgctct atggcgccat cgatggcagc ggaggcttct accacaaccc gatcgaagtg
     1201 gagagccgtt cgcgcatgaa cgtgccgttt accatgcagg acgatgcgct cgacgccgta
     1261 ttcctcaaac aggcggaagc cgccgggctg cattcgctga aaggccacaa atcggtaggc
     1321 ggcctgcgcg cctcgattta caatgccatg ccgctgcaag gtgtgcaagc gctggtggat
     1381 ttcatgcagg aatttgcccg gacccacgga taggaatgag cgacgaactc aaaaaatgtc
     1441 gtgaacagat cgacgcgatc gatgaccagt tgctcaagct gatcagtgaa cgcgccgggc
     1501 tggcacatgc catcggcagc ctgaagaacg gcgggccgat ctatcgtccg gagcgcgagg
     1561 cgcaggtgct gcgccggctg ctggagcaga atccagggcc gctttcagac gaggcagtaa
     1621 ccgccatctt ccgtaccgtg atgtccaatt gccgcgcgct ggaaaaagaa cttgccgtgg
     1681 cctttctcgg tccgctcggc acctatagcg aggaggccgc caacaagcag ttcggcgggc
     1741 tttcatcgcc ggtgcagtgt gccaccatcg acgaggtatt ccgcacggtg gaaaccggac
     1801 atgccgacta tggtgtggtg ccggttgaga actccaccga gggcgccatc ggccgtacgc
     1861 tggacctgct gttgcagacc gatttgcagg tgtgcggcga gatcgatttg ccggtgcacc
     1921 ataacctgct gtccggggct gccgacaaat cttctgtccg catcgtctat tctcacgcac
     1981 agtcgttggc gcagtgccac gaatggctca acgccaacct gcccggtgtc gagcggcagg
     2041 cggtatcgag caactcggaa gcttcgcgcc tggcagcgga gaatcctgcc agcgcagcca
     2101 ttgccagcaa gcgggcaggg gagttgttcg gtctcaacct gctggcggaa aacatcgagg
     2161 acgacccgcg caataccacg cgctttctgg tcgtcgctga ccacgatgtg gcaccgtccg
     2221 gcagggacag gacctcgctc gccatgtcta cacgcaacgt accgggcgcc attgctgacc
     2281 tgttggcgcc tttggctgcc aacggcattt ccatgagcaa gctggaatcg cggccttccc
     2341 acatcggtat gtgggaatac gtgttcttcg tggatatcga aggccaccgc agcgacccca
     2401 aggtggttgc ggcgctggag atgctgcgtg acaaggctgc tttcctgaag gtgctcggct
     2461 cctatccggt cgcggtggtt tgatgttgca ggagcgccgc cctcggcgcg atttttccat
     2521 gatcgcggcg cgccgccgct cctgcatcag cctttacgaa ccctcttaac gccctttcag
     2581 tttcaactgt tgcaagatca tggtcgaaat ctcttccacc gagcgggtgg tggaatccag
     2641 ccagggaatg ccttcgcgac gcatcagggt ttccgcctgg cgtacttcat tccggcagtt
     2701 gttcagagag gcgtagtgac tgtcgggacg gcgttcgccg cggatgcggt gcaggcgctc
     2761 cgggtcgatg gtgaggccga acagtttgct gcgataactg gccagatctc ccggcagtac
     2821 catgcgttcc agatcttccg ggatcagcgg gcagttggcg gcgcgcacgc cgaactgcat
     2881 ggcgagatag aggcaggtgg gagtttttcc gctacgtgag acgccgacca ggaccacgtc
     2941 ggctttttcc aggctggcgg gggtcaggcc atcgtcgtgg ctgagggtgt agttgatggc
     3001 gtcgatgcgg ttgagatatt cgctgccgac agcgcggtgc gacatgccga ctgtctgtgt
     3061 cggcaactgt ccgaactcga ccgacagcgg ctggatgaag gtgccgaaca gatcaaggca
     3121 aagcgcgccg ctgtcttcga acatctggcg gattttcggg ttaaccagcg tcagcaccag
     3181 gatggggcgg gtgccgtctt cggcttgcgc ggcggcgata cgggcgagcg catcctgcgc
     3241 tttgtccgga ctatcgatga aaggcagccg tacctgacgg aactcgacgc cgtcgaagtg
     3301 cgccagcaga ctcataccca aggtttcggc agtgatgccg gtccagtcgg agatgaagaa
     3361 aacgctgcgt ttgggcatgg gctaatcttt aggcataatc gcattcatga taacaccatc
     3421 cgcttttccc aagacggccg gctccgacga ggcgatcatt gcgcgcagtc gtgaactgag
     3481 cccttttttt caccgcacgc tggaggccga cgctttgctg ttgccggcgc tgcagtccgg
     3541 cttgcatcag gttttcgatg cggaacaaat gcgcgccact ctggcggagg gcgcaggtga
     3601 cgacgaagat gcgttgaaac gcaagctgcg ccggctgcgc aagcaagtca tgctgcacct
     3661 gattgcgcgc gacctcaacg gtctggccga tctggcggag gtggtagaga cctgtacggt
     3721 gctggctgaa gtcacggtca tttccgccct gaaacacctc agccactggc agcgccagct
     3781 ttatggcgat cctgttggcg aaagtggcac ggtacagcag ttgctgattg ccggcatggg
     3841 caaactgggc gggcgcgaac tcaacgtgtc gtccgacatc gatctcatct tcgcctacga
     3901 agaggatggc gaaaccaacg gcgcgcggca gatcagcaac catgagtatt ttgcccgcct
     3961 tggcaagaag ctgatcgccg ccatcggcga gaccacggcg gatggcttcg tttttcgggt
     4021 agatatgcgg ctccgtccct atggcgactc cggcccgctg gtgggaagtt ttgccgcgct
     4081 ggaggagtat taccagacgc agggacgcga atgggagcgc tacgcctgga tcaaggggcg
     4141 ggtcatcgcc gggccggagc aggcgttgca ggatttgctg cggccgtttg tattccgcaa
     4201 gtatctggac tacggcgcct ttgcttccat gcgcgacctc aaggcgcaga tcatgcgcga
     4261 agtggcacgg cgcgacatgc acgacaacat caagctcggc ccaggcggca tccgcgagat
     4321 cgagttcatc gcccaggtgt tccaactgat tcgcggcggc caggatcgag aattgcaaat
     4381 ccgccctacg ctgcaagtgc tgcaactcct ggagaagaaa agcctgctgc cgccggaaac
     4441 tgttgcggag ctgagcgcgg cctacgtgtt cctgcgcaac ctggaacatc gtcttcaata
     4501 tctgcaggat gcgcagacgc aaatgctgcc ggctgatgac gagaaccgcg cgcgcatcgc
     4561 ggccggtatg ggctttgccg actgggctgg ttttctgcag gagctcggcc gccaccgtgc
     4621 cagcgtcgaa cgccacttca gtcaggtgtt cgacgaaccg gagggcgaac agacccattc
     4681 cctcgccggt ttgtgggaag gctcgtcgag cgaggagggg ctgcagcagc agttggcggc
     4741 gctcggctac cgcaatacag ccgaagcctg gcagcgactc aacgggttac gctctgcttc
     4801 ccgctacaag cagttgcctg ccgccagccg ccagcgattt gatgcgctgg tgccgcgcgc
     4861 gctggaagct tctgctcggc acagcaatgc tgacgaaacg ttggcacgga tgttgaccct
     4921 gctggaagcg atctgccgcc gggccagtta tctggcgctg ctggctgaat atccgcaggc
     4981 gctggacgcg gtggcacggc tgtgttctgc cagcccatgg ctggcggcct acctcactca
     5041 gcatcccatc ctgctggatg aattgctgga tacgcgcagc ctttatgccg cgccggattt
     5101 ctccgcgctg cgcgacgatc tggaaagtcg tctggccgac tgcggcgagg acgtggagcg
     5161 caagctggac gtgcttcgcc acttcaagca cgcgcagact ttccgcttcg ctgcgcagga
     5221 tctgactggc gagttgccgc tggaaacact gtcggacgac ctcagcgcat tggccgacct
     5281 ggtgctggat gccgtcattc gccatgcctg gccggggctg cgcaaccggc accgggaagc
     5341 gccagccttt tccatcatag gctacggtaa actggggagc aaggagctgg gctacgcctc
     5401 cgacctcgat atcgtgtttc tctacgacga tgtggcagac aatgcgggtg aggtctatgc
     5461 ccgttttggc cagcgcatca acaactggct gaacagcctg accccagccg ggctgttgta
     5521 tgaaaccgat ctacgcttgc gtccggacgg cgccagcggg ctgctggtga gttcggtcga
     5581 ggcgtttact gaatatcaga aaaacaaagc ctggacttgg gaacaccaag cgctgacgcg
     5641 ggcacgtttt tgtgccggag ccccggccat tggtaatgct tttgagcagg ttcgcctcga
     5701 catcctgcgc cagccgcgcg acctcgccaa gttacgcgaa gaggtcgttg ccatgcggca
     5761 gaaaattcac gatggtcacc ccaataacac tccgctattc gacatcaaac acgatgccgg
     5821 cggcatcgtg gacgtggagt ttatcgtgca gacgctggtg ctggcttacg ccagacaata
     5881 cccccaactg gctgaaaacg tcggtaactt ggccctgtta ggtcgtgctg gggaggttgg
     5941 tctgatcctg cctgatctgg cgcgaagcgc agctgatgct taccgtgact atcgcaacgc
     6001 acaacatgtg cagcgcctgc agggcgcgga aaaatcacgg gtggatagag aaccattcgt
     6061 gcgggcaatc gcggctgtaa aaacgctatg ggatcaagtt ctgggtgatg ggctgtcaaa
     6121 atagaacggc ccgcgatcgc gggccgttgc aaagaatatt aacagtgttg cttaaggccg
     6181 atccaggctg cccatggact tgggatgggt cacgatgccc cagggcatgt ccttgtcgcc
     6241 gatgcgcttg gtgacttcca gtttctgtgt atcgatcacc accacctcgt tcgatttccc
     6301 gcaggccagc agaatgttct ggtcgtccgg ggtgaagctg aagtgccagc aacggtcgcc
     6361 ggtggggatg tccttgatct tttcgaaggt gcccgcgtca tatacctgta gcgccttttc
     6421 cttggaggag gcgacataca ggcgcttgcc atcgcggtca aaggacacac cgtaaggcga
     6481 cttgccggtg tcgatggatt tcaccgggtt cagcgtgttg tccaggatca gcagcttgtc
     6541 cgacatctcc agggtggaca catagaactt gccgtccggc gaagtcttga tgccgcgcgg
     6601 acgctcgccg taggagccta ccgggatgct cttcagcaat ttgcccgact tcatgtgatg
     6661 tacggtgatg gaattatcgg attcgttggt gacgatcatg cgcttgccgt ccaaggagaa
     6721 ttccacgccc tctgtttccg gcttgccggt gatatctttg acgaccttgc cctttttcag
     6781 gtcaacgatg gcgatatggc cgggcaggtc atcttcatca tcgttatcct tggcaggctc
     6841 tgcgccgggc ttgggaggcg ggccgccctt ggagctcggc tcgtaggtaa cgtaggccct
     6901 gtcgccgtag acgcgcacga attcggggtt cttgccgata ggtatatgct tgaccacctt
     6961 gccgctggcg gtatcgatga tggagatatt gccgtcgtcc ttgttggcgg tgaccagcca
     7021 tttgccgtcg gcggtgatgc cgataccgcg cggacccttg gcaccgatgt ccagagtgcc
     7081 ggtggtttcc atggtggcca ggttaatgac ggtgacgccg ccatcctgat tggatacata
     7141 ggccacgccc ttgctatcgg ctagcacatt ggtaataccc agaagggcaa gaaaaagggt
     7201 gatcgggagc ctcttggcga aggggtggaa cttcatattg tgcttctcct cacaaatgtt
     7261 gaaattgcgc cggacggcga gtttaacgta attcacgttg attgggcgct accagcaagc
     7321 caaagttcca ttctggccat gaccagcaga gcaaaaactt gtgcaaaatt cgagccaggg
     7381 agatcaagaa gaaaagtatt ataataaaac aacatgttat ataaaatttt gcgaggatgg
     7441 aggtggcgag ctggtcgata tcagccgccg gctggttgca gccaaccttg atgtcagcct
     7501 tcatttggcc atgatggcca gggctttcag tagcggcgca tccgaaacct tgatgatccc
     7561 ctcgaacggg tggctcattt caatcacgag aaaaagcgcc gccgagatcg aggctccgct
     7621 gattatcaag gccaaggtgc tggtcaggtt gtgttgcgac aacaggctga aactcatgaa
     7681 caggatgccc agccataaga taagaaccag caggaatacc ggggggaccg agctttgcga
     7741 ttcctcggtc agcagcaagc tggattgcag aaggtcgcta accagctgct gtaactgtgg
     7801 ctggaattgg cgctgctggt cggaggccgg ggtgagcgca cccaggccgt ctgccatttt
     7861 ccccagactc tgcgccatgg tggacgattc agtgcctttt ttctccagac cgtcctccgg
     7921 ccagatgcgc tgtatggcgc cgacaaggcc ctcgcgcaac tgagcccgaa tctcgctggt
     7981 ttcgggtccg tagcgcacca gggcgcgatc cagcgcgatt atttttgcgc cactctggac
     8041 aagttgatta ttgatctcgt cgaagttgga cttggtcgag ccgaccaaca agcccagcac
     8101 cagcgctacc agggtggcga tcagaccgga cgccagcttg atggcgtccc tcgagtcttc
     8161 gcgcagatga tgatccggca agcgatcgcg caggattgcg cccaagacga tgccggcgat
     8221 gatgcagatc agggtaacga taaaaacact ccaagagctc atctatttct ctcctgatag
     8281 ttaagattgc aaccgacttg aggcgttcgt ggcatttttc aatgcttttt ctgtaatatc
     8341 gcgacacagg ctgatcaggc gctggtaaaa caattgccag ttttcacggg catcaacttt
     8401 tgccttgtct taggtaagtc gttgcaa
//
LOCUS       DAOMFA010000069         8395 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_982_length_8395_cov_5.076259, whole genome shotgun sequence.
ACCESSION   DAOMFA010000069 DAOMFA010000000
VERSION     DAOMFA010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 8395)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8395)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8395
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_982_length_8395_cov_5.076259"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2250)
                     /gene="ileS"
                     /locus_tag="PKW44_04870"
     CDS             complement(<1..2250)
                     /gene="ileS"
                     /locus_tag="PKW44_04870"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HQR50950.1"
                     /translation="MTDYKDTLNLPDTPFPMRGDLARREPAWVKGWQERKLYQKIREA
                     RRGAKKFILHDGPPYANGDIHIGHAVNKILKDIIVKSKTLSGFDAPYVPGWDCHGLPI
                     ELVVEKNHGKNIEPAKFRELCREYAQAQVERQKADFIRLGVLGDWDHPYLTMDFRTEA
                     DIMRALGQIYANGYLYQGSKPVHWCVDCGSALAEAEVEYEDKNSPAIDVGFRAVDNAA
                     VCKAFDIGSTPEPISAVIWTTTPWTLPANQAVCVHPELTYDLIQTPHGLLILARELAE
                     AAIRRYGFETVEVIANCQGAALEHLKLQHPFFERQVPVICGDHVTTDAGTGLVHTAPA
                     HGLEDYAVGMRYDLPVDNPVDDNGKFFASIPLFGGMSVWDANKAVIEALAANHNLLAS
                     ARLNHSYPHCWRHKTPVIFRATHQWFIGMGQHGKDGQTLRGIANAAVEHTQFYPAWGR
                     ARLEAMIKNRPDWCVSRQRNWGVPMPLFVHKESGLPHPDTLALLEKVCLQVEQQGIEA
                     WFSLDGAPFLAQHAPQDAGQYKKVTDTLDVWFDSGATHAAVLKRRAELQHPADLYLEG
                     SDQHRGWFQSSLLTGCAMDGRAPYEALLTHGFVVDGSGHKMSKSKGNVIAPQKVMDTY
                     GADILRLWVASTDYSGELTISDEILKRVVESYRRIRNTLRFLLANIADFDAQSDMLPV
                     EQWLEIDRYALHLTQRLQDGILADYERYEFHLGMQKFVGFCSEDLGGFYLDILKDRLY
                     TAGADSRARR"
     gene            complement(2247..3161)
                     /locus_tag="PKW44_04875"
     CDS             complement(2247..3161)
                     /locus_tag="PKW44_04875"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HQR50951.1"
                     /translation="MQIFRHIPATASGPVALTIGNFDGIHCGHRAILDALRQKAQVRG
                     LTPSVMTFEPHPREFFSPATAPSRLTSLREKLELFEELGMERVYLLRFNHRFAAISAQ
                     DFMQHILRTLDTRLILVGEDFRFGAARAGDVAGLRAAGFGVESVSTVELDGQRISSTA
                     VREALAAGELAQAAALLGRAYSISGHVAHGDKLGRLLGYPTANIHMLHERPPLYGIYA
                     VKLRGIDDEDLPGVASLGVRPTMKHNGRPTLEVHLFDFNRDIYGRHVRVQFLKKLRDE
                     QKFPDLETLKRQIEADERAARDYFKNEK"
     gene            complement(3172..4707)
                     /gene="murJ"
                     /locus_tag="PKW44_04880"
     CDS             complement(3172..4707)
                     /gene="murJ"
                     /locus_tag="PKW44_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004177124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HQR50952.1"
                     /translation="MNLLKALATVGSMTFLSRILGFVRDTLIARVFGAGVYTDAFFIA
                     FKIPNLLRRLFAEGAFSQAFVPVLAEYKNRRGYEETHTLVNHVATLLGLAVFAVTVLG
                     VLAAPWVVYLSAPGFHTSPDKFQLTVDLLRITFPYIFFISLVSLAGGVLNTWSRFSVP
                     AFTPVLLNLSFIVAALFFAPYFDPPVMVLAWAVFAGGILQLAFQIPFLLKIGLVPRFS
                     LNLHDEGVWRILRLMGPAVFGVSIAQLSLLINTIFASFLETGSVSWLYYADRLMEFPT
                     GMLGVALGTILLPSLSKSYADKSHSEYSQLLDWGLRLTFILAVPAAAALAVLAVPLIA
                     TLFRYGEFNIHDLWMTRQALMAYSLGLLGLILIKVLAPGFYARQNIKTPVKIAIFTLL
                     ATQVMNLIFIWPFKHAGLALAISLGACLNAGLLFYHLRRHDIYQPQPGWLPFLLKLAT
                     AVGAMGLVLWAGMGSTDAWLQYHVMQKLLHLSLLVTGGVGTYFGVLWLLGFRLQHFTR
                     RAT"
     gene            4670..5095
                     /gene="rpsT"
                     /locus_tag="PKW44_04885"
     CDS             4670..5095
                     /gene="rpsT"
                     /locus_tag="PKW44_04885"
                     /inference="COORDINATES: protein motif:HMM:TIGR00029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HQR50953.1"
                     /translation="MLPTVASALRRFIKPVEKRGGLSYHGNADTALQKPVRVCMIARF
                     EFCTISGVFMANSAQARKRARQAVKQRAHNASLRSELRTAIKKVIKAVEAGDKAAAQA
                     AYKDNVSVIDSIADKKIIHKNKASRHKSRLSAAVKAMAA"
     gene            5487..6551
                     /locus_tag="PKW44_04890"
     CDS             5487..6551
                     /locus_tag="PKW44_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase/NADH oxidase"
                     /protein_id="HQR50954.1"
                     /translation="MAKLFTPFHLRGLELKNRIVVSPMCQYSAEGGMPNLWHLVNYGS
                     RAVGGAALVVVEATAVCPEGRITPGCLGLWSDAHTAAFRPITEFIKQQGAVPAIQIAH
                     AGRKAACDAPWNGGKFLPIGCGGWQTVAPSAIPFTPAHGTPHELSLHEIDGVVELFAD
                     AAHRALAAGFEVAEVHCAHGYLLNEFLSPLSNKRDDKYGSSLENRCRLPLKVAKTIRD
                     IWPQQWPVLVRISATDWVDGGWDLEQSVQFARWLKEIGIDLIDCSTGGLVLDAKIPVG
                     PGYQVPFAAGVRHGAGIATGAVGMITSPQQAERILLDEQADLIFLARELLRDPYWPLH
                     AAHKLGVDVAWPNQYVRAKL"
     gene            6548..6742
                     /gene="iscX"
                     /locus_tag="PKW44_04895"
     CDS             6548..6742
                     /gene="iscX"
                     /locus_tag="PKW44_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009238557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HQR50955.1"
                     /translation="MKWTDVQRIAEELHDKFPGTDPARINFVDLHNRVVELDGFDDDH
                     HRGGEKVLEAIQQAWIDEAG"
     gene            6795..7601
                     /locus_tag="PKW44_04900"
     CDS             6795..7601
                     /locus_tag="PKW44_04900"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR50956.1"
                     /translation="MKLLLVLLLMISCQAFAQQEVEVIPLQHRTLEQMLPVVTPFVEP
                     GGVASGMNGQLIIRASRQNIEEIKALLARLDTPAKRLKITVSNNIAADRAAEEASVSG
                     RVIVGDRTEAHIQGEFSASTGNADRHLKQSLQVLEGSSGYIVVGQSLPIPLHQVVFNP
                     NGAVITDTLEYHDIGSGFYATPHLNGDRVTLSISPQMETPIKGTHGAVGLQRLSTTVS
                     GKLGEWIELGGTLADGNSRRSEIASYSNSSASENRSVWLKVEEVIKPPHQ"
     gene            complement(7768..8235)
                     /locus_tag="PKW44_04905"
     CDS             complement(7768..8235)
                     /locus_tag="PKW44_04905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR50957.1"
                     /translation="MAAHFEAVHAHGNSEKALRGYTAGLRLLAAYSAAELLGEAIGSN
                     VTSWKIPDSDLSAALRRVMLRPSADSNGLFAQKGLREKLQLFMDGDIDVRIPATALRV
                     MVAHGSFTPTGTDSLTKAGAEALQRLSDVLLQECEKRFSVWLHEMLVEHGEAA"
BASE COUNT         1778 a         2527 c         2501 g         1589 t
ORIGIN      
        1 gcggcgtgcg cgggaatcgg caccagcggt gtagagccgg tctttcagga tatcaaggta
       61 aaaaccgccc aggtcttccg agcagaaacc gacgaacttc tgcatgccaa ggtggaattc
      121 gtagcgttcg taatcggcca gaatgccgtc ctgcaagcgc tgcgtcaggt gcagggcgta
      181 gcggtctatc tccagccact gctccaccgg caacatgtcg ctctgggcat cgaagtcggc
      241 gatgttggcc agcaggaaac gcaaggtatt acgaatccgg cggtagcttt ccaccacgcg
      301 cttgaggatt tcgtcggaga tggtcaactc accggagtag tcggtggaag cgacccacag
      361 gcgcaggatg tcggcaccgt aggtatccat taccttctgc ggcgcgatga cgttgccctt
      421 cgatttgctc atcttgtggc cggaaccgtc caccacgaag ccgtgcgtca gcaaagcctc
      481 ataaggcgcg cggccatcca tggcgcaacc agtgagtaag ctggactgga accagccgcg
      541 atgctggtcg gagccttcca gatacagatc ggccgggtgc tgcagctccg cacgacgctt
      601 cagcacagcg gcatgggtgg cgccggagtc gaaccacaca tcgagggtat cggtgacttt
      661 cttgtactgc ccggcatcct gcggcgcatg ctgggcgagg aagggggcgc catccagact
      721 gaaccaggct tcgatgccct gttgttcgac ttgcagacag accttttcca gcagcgccag
      781 ggtgtcggga tgcggcaaac cgctttcctt gtgcacgaac agcggcatcg gcacgcccca
      841 gttgcgctgg cgcgatacgc accagtccgg gcggttcttg atcatggctt ccagccgcgc
      901 cctgccccat gccgggtaga actgcgtatg ctccactgcc gcgttggcga tgccgcgcaa
      961 ggtctggcca tccttgccgt gctggcccat gccgatgaac cactggtgcg tggcgcggaa
     1021 gatcaccggc gtcttgtggc gccagcagtg cggatagctg tggttaaggc gagcggaagc
     1081 cagcaggttg tggttcgcag ccaaggcttc gatcaccgcc ttgttggcat cccaaacgct
     1141 catgccaccg aacagcggga tagaagcgaa gaacttgccg ttgtcatcta ccggattgtc
     1201 caccggcagg tcgtagcgca tgccgacggc atagtcttcc aggccgtgcg ccggcgccgt
     1261 atgtaccagg ccggtaccag catcggtggt gacgtggtcg ccgcagatca ccggcacctg
     1321 gcgctcgaag aacgggtgct gcagcttgag gtgttccagc gccgcaccct ggcagttggc
     1381 gatcacttcc accgtctcga agccgtagcg ccggatggca gcctcagcca gctcgcgcgc
     1441 caggatcagc agaccatgcg gggtctggat caggtcgtag gtcagttcgg gatgcacgca
     1501 caccgcctgg ttggccggta gcgtccacgg cgtggttgtc cagattaccg cggagatcgg
     1561 ttccggtgtg ctgccgatgt caaaggcctt gcacacggcg gcgttgtcca ccgcgcggaa
     1621 gccgacgtcg atggccggcg aattcttgtc ttcgtactcg acctcggctt cagccagtgc
     1681 cgaaccgcaa tccacgcacc agtgcaccgg cttggagccc tggtacaggt agccgttggc
     1741 gtagatctga ccgagcgcgc gcatgatgtc ggcctcagtc ctgaagtcca tggtcaggta
     1801 cggatggtcc caatcgccca gcacgccgag gcggatgaaa tcggccttct ggcgctcgac
     1861 ctgggcctgc gcgtactcgc ggcaaagctc gcggaatttg gcgggctcga tattcttgcc
     1921 gtggttcttt tccaccacca gttcgattgg caggccgtgg cagtcccagc ccggcacgta
     1981 gggcgcgtcg aagccgctca aggtcttgga tttgacgatg atgtccttga gtatcttgtt
     2041 gaccgcgtgg ccgatgtgga tgtcgccatt cgcatagggc ggaccgtcat gcaggatgaa
     2101 tttcttcgcg cctctgcgtg cttcgcgtat tttctggtac agcttccttt cctgccagcc
     2161 cttgacccag gccggttcgc gcctggcgag gtcgccgcgc atgggaaaag gagtgtcggg
     2221 caggttcagc gtgtctttgt aatcggtcat ttttcgttct tgaaataatc tctggccgcg
     2281 cgttcgtcgg cctcgatctg ccgtttcagc gtttccaggt cgggaaactt ttgctcgtcg
     2341 cgcaactttt tcaggaattg cacgcgcacg tgccggccat aaatatcgcg gttaaagtcg
     2401 aacaaatgca cttccagcgt cggccgaccg ttgtgtttca tggtcggccg cacgcccagg
     2461 ctggccacgc cgggcaaatc ctcgtcgtca atcccgcgca attttaccgc atagataccg
     2521 taaagcggcg ggcgctcgtg cagcatgtgg atgttggcag tcggatagcc gagcagtcgc
     2581 cccagtttgt cgccatgtgc aacgtggccg ctgatgctat aggcgcggcc cagcaacgcc
     2641 gccgcctggg cgagctcgcc ggccgccagc gcttcacgca cggcggtgct ggaaatacgc
     2701 tggccgtcca actctaccgt cgacacactc tccacgccga agcctgccgc gcgcagacca
     2761 gccacatcgc cggcccgcgc cgcgccaaaa cggaaatcct cgccgaccag aatcagtcgg
     2821 gtatcgagcg tgcgcaggat atgctgcatg aagtcctgcg cgctaatggc ggcgaaacgg
     2881 tggttgaacc ggagcagata aacgcgctcc atgcccagtt cctcgaacaa ctccagcttt
     2941 tcgcgcagcg aggtaaggcg cgacggagcc gtggcgggcg agaagaactc gcgcggatgc
     3001 ggttcgaagg tcatcacgct cggcgtcagc ccccgtacct gcgccttctg tcgcagtgca
     3061 tcgagaatag cgcgatgtcc gcaatggatg ccatcgaaat tgccgatggt cagggcgacc
     3121 gggccggaag ctgtggcagg gatgtggcga aaaatctgca taccaaaacc gttatgtcgc
     3181 acggcgggta aagtgctgca atcggaaccc caatagccac agcacgccaa aataggtacc
     3241 gacacctccc gtcaccagca gcgacaggtg cagcagtttt tgcatgacat ggtattgcag
     3301 ccaggcatcc gtgctgccca tgcctgccca gagcaccagc cccatggcgc cgaccgccgt
     3361 ggccaacttg agcaggaacg gcagccagcc gggctgcggc tggtagatgt cgtggcgacg
     3421 cagatggtag aacagcagcc cggcgttgag gcaggcgccc agcgaaatag ccagcgccag
     3481 cccggcgtgc ttgaacggcc agatgaagat gaggttcatc acctgtgtgg ccagcagggt
     3541 gaagatggcg atcttgaccg gcgtcttgat gttctgccgc gcatagaatc cgggcgccag
     3601 caccttgatc aggatcagcc cgagcaggcc gaggctgtag gccatcagcg cctggcgcgt
     3661 catccacagg tcatgaatgt tgaactcgcc ataacggaac agcgtggcga tcagcggcac
     3721 cgccagcacc gccagcgccg cggcagccgg cacggcgagg atgaaggtca ggcgcagccc
     3781 ccagtctagc aactgggaat actcgctgtg ggatttgtca gcatagctct tggacaggct
     3841 gggcagcagg atggtcccca gcgccacgcc cagcatgccg gtggggaact ccatcaggcg
     3901 gtcggcgtaa tacagccagg acacgctgcc ggtttcgagg aacgaggcga aaatggtgtt
     3961 gatgagcagc gacagctgtg cgatggaaac gccgaacacc gccggcccca tcagtctcaa
     4021 tatgcgccac accccttcgt cgtgcaggtt gaggctgaag cgcggcacca ggccgatctt
     4081 gagcaggaac ggtatctgga acgccagttg cagtatgccg ccggcgaata ccgcccacgc
     4141 cagcaccatc accggagggt cgaaataagg agcgaaaaac agtgcggcga cgatgaaaga
     4201 gagattgagc agcaccgggg taaacgccgg caccgagaaa cggctccagg tgttgagcac
     4261 cccgcccgcc agcgacacca gcgagatgaa gaagatgtag gggaaagtga tgcgtagcag
     4321 atcgacggta agctggaact tgtccgggct ggtgtggaag ccgggcgcgc tgagatacac
     4381 cacccacggc gcggcgagca cccccagcac cgtcactgca aatactgcca gcccgagcag
     4441 ggtggcgacg tggttcacca gcgtgtgggt ttcctcgtag ccacgccggt tcttgtattc
     4501 cgccagcacc ggcacgaaag cctgcgaaaa tgcaccctcc gcgaacagcc ggcgcagcag
     4561 gttgggaatc ttgaaggcga tgaaaaaggc gtcggtatag accccggcgc cgaacacccg
     4621 cgcgatcagg gtgtcgcgca cgaacccgag gatgcgggaa aggaacgtca tgctgccgac
     4681 cgtggccagc gctttgagaa gattcataaa accggttgaa aaacgaggtg ggctatctta
     4741 tcacggcaat gcggatacag ccttgcaaaa gccggtgcgt gtctgtatga ttgcgcgttt
     4801 tgaattttgc acaatttcag gagttttcat ggctaacagc gcacaagcaa gaaaacgcgc
     4861 ccgtcaagcg gtcaagcaac gcgcccacaa cgcgagcttg cgttccgaac tgcgcaccgc
     4921 gatcaagaag gtcatcaagg ccgtcgaagc cggcgacaag gctgccgccc aagctgctta
     4981 caaagacaac gtcagcgtga tcgacagcat cgccgacaag aagatcatcc acaagaacaa
     5041 ggcctcccgc cacaagagcc gcctgtccgc cgccgtcaag gcaatggctg cttaattctt
     5101 gtagttgccg ttccagcaaa aagccaaccc gcgggttggc ttttttgttg ccctcattgc
     5161 cacttacctc ggcttccgtc tgcaaacccc ggcaacctgg atatttgtgg tcgaatgcaa
     5221 ccattcggct gtattaagcc atgtcattta ccggcacgca cccatcataa tggcatacca
     5281 tattgtttta taacgattat accgttatgg cacggggatt gcgtattgaa tatcagcaac
     5341 agctttctcc aatgtgctcc ccttgagccc gccgtgcgca aatgtcacaa tccaccggcg
     5401 ggtctttttt cgcccaatgc acgctataat tttcagcagc agtcatgcgc ggcgatcccc
     5461 caagcacatc accctaggat tccacattgg caaaattgtt cacccctttc cacctgcgcg
     5521 gcctcgagct gaaaaatcgc atcgtggtct cgcccatgtg ccagtactcg gcggagggcg
     5581 gcatgcccaa cctctggcat ctggtcaatt acggcagccg cgcggttggc ggcgcggcgc
     5641 tggtcgtggt ggaagcgacc gccgtttgcc ccgaaggtcg catcacgccg gggtgcctgg
     5701 gattgtggtc ggacgcgcat acggccgcat ttcgcccgat taccgagttc atcaagcagc
     5761 aaggcgcagt gcctgccatc cagatcgctc acgccggacg caaggccgcg tgcgacgcgc
     5821 cctggaatgg cggcaaattc ctgcctatcg gttgcggtgg ctggcagacg gtagcgcctt
     5881 ccgccattcc gttcactccg gcgcatggca cgccacacga actgagcctt cacgaaatcg
     5941 acggcgtggt ggaactgttc gccgatgccg cgcatcgcgc gctggccgcc ggtttcgaag
     6001 tggccgaggt gcattgcgca catggctatc tgctcaacga attcctgtcg ccgttgtcga
     6061 acaagcgcga tgacaaatac ggaagcagtc tggaaaaccg ctgccgactc ccattgaagg
     6121 tagccaaaac tatccgcgat atctggccac agcaatggcc ggtgctggtg cggatttcag
     6181 cgacagactg ggtagacggc gggtgggatc tggaacaatc tgtccagttc gcgcgatggc
     6241 tgaaagaaat cggcatcgac ctcatcgatt gctcgaccgg cggccttgta ctggatgcca
     6301 aaattccggt cggcccaggt tatcaggtgc catttgctgc cggtgtgcgc cacggcgcag
     6361 gcatcgccac cggcgccgtg ggcatgatca cctcgccgca gcaagccgag cggatcctgc
     6421 tggacgaaca ggccgatctg attttcctcg cgcgcgaact gttgcgcgac ccctactggc
     6481 cgctgcacgc cgcgcataaa ctgggcgttg acgtagcctg gcccaaccaa tatgtgagag
     6541 caaaattatg aagtggacgg atgtacaacg catcgcagaa gaactccatg acaagttccc
     6601 gggaaccgat cctgcccgca tcaatttcgt cgacctgcac aacagggtcg tcgaactgga
     6661 cgggttcgac gacgaccatc accgcggcgg cgagaaggtg ctggaagcca tccagcaagc
     6721 ctggatcgac gaagccggct agaatttctc acggcagcct tgattgcggc acaaactgct
     6781 tgggaactcc aaacatgaaa cttctgcttg tgttgctgct catgatttcc tgccaggctt
     6841 ttgcccagca ggaggtggaa gtcattccgc tgcaacaccg gacgctggag cagatgttgc
     6901 cggtagtgac cccgttcgtc gaacccggcg gcgtggcttc agggatgaat ggccagctca
     6961 tcattcgagc cagccgccag aacattgagg aaatcaaggc actgctggcc aggctggata
     7021 cccctgcgaa acgtctgaag atcaccgtca gcaacaatat tgctgccgac cgcgcagcgg
     7081 aggaagccag cgtgtcgggc cgcgttatcg tcggcgatcg caccgaggct cacattcaag
     7141 gggaattttc cgcatcaacc ggcaatgcag accgccatct caagcagagc ctgcaagtgc
     7201 ttgaaggcag cagcggctat atcgtcgtcg gccaatcgct acccatcccg ttgcatcaag
     7261 tggtttttaa ccccaacggg gcagtcatca ctgacacgct ggagtatcac gatatcggca
     7321 gcggtttcta cgcgacccct caccttaatg gcgacagggt gacgctttcc atcagtccgc
     7381 agatggaaac cccgataaag ggcacccatg gtgccgtggg cctccagagg ctatccacga
     7441 ccgtctcagg caaactggga gaatggatcg aactgggtgg cacgctcgcg gatggcaaca
     7501 gccgcaggag tgaaatcgcg tcatactcca actccagcgc ttcggaaaac cgaagcgtct
     7561 ggctcaaggt ggaggaggtc attaaaccgc ctcaccaata aatttggcgt tccagcgcag
     7621 gcgaatccgg ctacaacttg actacggtaa caacaaaata aaaacggccc acatcactgt
     7681 gggccgcctg gatactggtg gtggagtcta gcactcggtc gaactttcct caataacgcc
     7741 tgcgggttgg tgcggaagcg cacggagtca tgcggcctca ccatgctcca ccagcatctc
     7801 atgaagccag acggaaaagc gcttttcgca ttcctgaagc aaaacatctg acaaccgctg
     7861 tagcgcttcg gcacccgctt ttgtcaggga gtccgttcct gtcggcgtga agcttccatg
     7921 ggccaccatg acgcgcaacg ctgtggcagg aatgcggaca tcaatatctc cgtccatgaa
     7981 cagctggagc ttttctcgca gtcccttttg tgcgaaaagc ccgttggaat ctgcggaagg
     8041 tcgcagcatt actcgtcgca acgctgccga caagtcggaa tcggggatct tccaagatgt
     8101 gacgttgctc ccaatggctt cgccaagcag ttcggcggca ctgtaagccg caagcaaacg
     8161 cagtccagcg gtgtagccgc gcagggcttt ttcgctgttc ccatgtgcat ggactgcttc
     8221 aaagtgcgca gccaagcgat gtcttgcata aaagcacact ttttcagtgg cttattggca
     8281 aacagcaaga gatcgaggcg atcattcacc ttggcgcctg aagcagcacg gtagaacaag
     8341 acgcaaacta cctgcttgaa aataactacc gctacacccg cacacttgct gaata
//
LOCUS       DAOMFA010000070         8248 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_1013_length_8248_cov_4.590382, whole genome shotgun sequence.
ACCESSION   DAOMFA010000070 DAOMFA010000000
VERSION     DAOMFA010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 8248)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8248)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8248
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1013_length_8248_cov_4.590382"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..193)
                     /locus_tag="PKW44_04910"
     CDS             complement(<1..193)
                     /locus_tag="PKW44_04910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR50958.1"
                     /translation="MRRVLLLLRDLLLVLCVRLVIRLLFIALPGATHHRTPDRTNRRT
                     LAGIPGNRPNHSTSERATGC"
     gene            434..1255
                     /locus_tag="PKW44_04915"
     CDS             434..1255
                     /locus_tag="PKW44_04915"
                     /inference="COORDINATES: protein motif:HMM:NF018352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HQR50959.1"
                     /translation="MNKSKLIYGSIGLAAVLLAPVAHAEITIPMEGVDVNMVGIAVGS
                     VPDYWGSSHNEVAAGPYGRYQFEGTQRYIQLLGPVFTANLLNDTNWRLGPLIKYRSAR
                     DSGVDDKVVSEMDKVDSAIEGGVFVGYKLALSDTPMHQVNFNADFESGKNGAEGHLSA
                     NYFQPFSKTIIGNIGLGLTYGNDKFVETYFGVTSAHDVSLYPSLGGRPYNTSSGVVGY
                     NIPFGISAFLTEEWLLSVGGRYERLVGDAKDSPVVDQRGDANQWIGGLGLAYVFK"
     gene            1442..5332
                     /gene="purL"
                     /locus_tag="PKW44_04920"
                     /gene_synonym="purI"
     CDS             1442..5332
                     /gene="purL"
                     /locus_tag="PKW44_04920"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HQR50960.1"
                     /translation="MLRLRGSAALSPFRLDKILAALKVAAPRISHLYTEFWHFAWAEG
                     ELSVDQQDTLQKILTYGPTMAEEAPIGEMFLVIPRPGTISPWSSRATDIARHCGLEMM
                     QRLERGVAYYAATADGSPLTEEEKRALRPLIHDRMTEAVFPTLDDAARLYHTAEPAPL
                     SSVDILGGGKAALQAANSDMGLALSPDEVDYLVENFTRIGRNPTDVELMMFAQANSEH
                     CRHKIFNADWVVDGVEQSQSLFDMIRNTHKLNPGKTVVAYSDNASIVQGEKTRRFYPQ
                     PDGRYEYVEEEMHFLMKVETHNHPTAIAPFAGAATGAGGEIRDEGATGSGSKPKAGLT
                     GFSVSNLNIPGFKQPWEADYGKPGRIASALQIMMDGPLGGAAYNNEFGRPNIAGYFRT
                     LELAAAGEVRGYHKPIMLAGGIGSISAQHSKKNPIPPGAALIQLGGPAMLIGLGGGAA
                     SSMDTGSNAENLDFDSVQRGNPELQRRAQEVIDRCWQLGERNPILSIHDVGAGGLSNA
                     FPELVNDAGRGAVFQLRDIHNEEPGMSPRAVWCNEAQERYVLAVSKDDLYRFKEICER
                     ERCPFAIVGEATEERHLAVEDSHFNNKPVDMDLAVLLGKPPKMTRDVAHLKRQLPEFD
                     AASIDLAEAVDRVLRLPGVADKTFLITIGDRSVTGLIARDQMVGPWQVPVADVAVTLA
                     GYETYRGEAFAIGEKAPLALIDAPASGRMAIGEAITNIAASLIEDIGEIKLSANWMAP
                     AGHPGEDAALYDTVRAVGMELCPQLGISIPVGKDSMSMKTVWEEGDEKKAVTAPISLV
                     VTAFAPTPDARKTLTPQLRTDLGPTKLILVDLGAGRNRLGGSALAQVYGAVGSVAPDV
                     DDAARLKAFFAAVQQLNRDGKILAYHDRSDGGLVATLCEMAFAGHCGLDIVLDGIVGN
                     TSVNAALFSEELGAVLQVRAGDMEMVCTSLDAAGLGGCVYVIGATSEGEHVRIQHAGR
                     EVFGERRVVLQRRWSETTFHMQSLRDNPACARQEYDRLFDVRDPGLHAQLSFDLKEDV
                     AAPYINTGHRPAMAILREQGVNGQVEMAAAFDRAGFETFDVHMSDIITGRVSLRDFKG
                     FVACGGFSYGDVLGAGEGWAKSILFNPRASDEFAAFFQRGDSFALGVCNGCQMMSNLH
                     SIIPGTEHWPHFVRNGSEQFEARFTMVEVLDSPSLFFGGMAGSRMPIAVAHGEGYAEF
                     SSQEAVERALAAQLVSVRYVDNYGNPTERYPANPNGSPQGITGLTTVDGRFSIMMPHP
                     ERVFRAVQHSWHPDGWGEEGPWLRMFRNARKFVA"
     gene            5290..5835
                     /locus_tag="PKW44_04925"
     CDS             5290..5835
                     /locus_tag="PKW44_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HQR50961.1"
                     /translation="MVAHVPQCTKICSLIIMTEIKMRLIQMFFMAAMLMLSSTVFAIT
                     DDEQVELSYAIEKGDAATVKKFLDNGLNPDDTAFAWSWLQVAANKNQLEIVKLMVERG
                     ANVNYKHPITKMTSVAFAAYNGNKEMVTYLISKGADPNIKLRGGVSLVRMSRDMGNQE
                     MADFLMQNGAKDDGCKEEKCF"
     gene            5876..6214
                     /locus_tag="PKW44_04930"
     CDS             5876..6214
                     /locus_tag="PKW44_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HQR50962.1"
                     /translation="MKARFRMLVLLLFAPLPSLAVSEGGHQRVLAASCAACHGTNGNS
                     VGGTPVLAGLDRSYFVLQMQAFRTGERSSTVMHTHAKGLTEEEIGLLAEYFSVEKRTA
                     SSSPKPLGDL"
     gene            6214..7476
                     /locus_tag="PKW44_04935"
     CDS             6214..7476
                     /locus_tag="PKW44_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HQR50963.1"
                     /translation="MMQRREFLKVLGVGAMASAVPGFASAAGKAQGRVVIVGAGYAGA
                     TAAKYLRLWGGDQLEIILVEKGTAFISCPLSNLVLGGSKRIGDLTFNYSGLDRYNIKR
                     LYAEVSGIDAAQRKVYVGDGDFLEYDRLIIAPGIDFMYESLPMLEIASAQEVVPHAWK
                     AGPQTVNLRKQLEAMPDGGVFLLSVPKAPYRCPPGPYERVSQVAFYLRNAKPKSKIIV
                     LDANPEIVSKKGLFTKAWAELYPGMVDYRPNSQVVEVDVAARTVKTEFETVKADVLNV
                     IPPQRAGRIAQQVGAANVDRRWCEVDFLTYESKAVPGIHIIGDSVSAALPKSAHMATS
                     QAKVCASAITTLLAGDTPDPQPVFANTCYSFVSDKLAMHVAHVYRYDPAKKIMLPAEG
                     GGVSDRPTELEGDYAQWWAKNIWSDVLS"
     gene            7476..>8248
                     /locus_tag="PKW44_04940"
     CDS             7476..>8248
                     /locus_tag="PKW44_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HQR50964.1"
                     /translation="MRRLPAFVLLTLIACLARADMASVVPVPNDPAVALQVPADFSIQ
                     AFARVNPAGGGYFRGPRFMAFGPDGNLYLSLGLDNKVVMLPDRDHDGRADEIATVADK
                     LNGPQGLVFVEGMLYVANQDGVVRLEQGDGKWPASEVKPIIGNLPTGGHTMKSLKLGP
                     DGLLYLNVGSSCNVCEESDPLRATLLRYSVDGKPAGALPSVGRHPSSPIFASGLRNTQ
                     GFAWHPATGALYATNNGADMRSDTKGGKPVDYLPPEHLNL"
BASE COUNT         1671 a         2343 c         2612 g         1622 t
ORIGIN      
        1 cgcagcccgt ggcgcgctcg gaggtgctgt gattggggcg attgccgggg ataccggcaa
       61 gggtgcggcg attggtgcgg tcgggggtac gatggtgggt ggcgccaggc aacgcaataa
      121 acagcaggcg aatgaccagg cgaacgcaca ataccagcaa caagtcgcgc aacagcagca
      181 aaacgcggcg caacagcaac aggactttca gcgcgcttat gcggcctgct tcgaaggtcg
      241 gggatatacc gtcaagtaag aatgatgacc gcgtgccgcg atgacgccat gcaaagattg
      301 agcgtcaggc ggacgcaaaa ctgcaacaat ttagcggaat aatccagtgg ggttgtttaa
      361 ttggtgctgg tcaaggatta agtaatataa tgcaggaaaa attggtaaga aattctactg
      421 aacggagcgt ttgatgaaca agagtaaatt gatttacggc agtatcggtc ttgcggcagt
      481 gctgctggcg ccggtcgccc acgcggaaat cacgattccg atggaaggtg ttgatgtcaa
      541 tatggtgggg attgccgttg gatccgtgcc ggattattgg ggctcttcgc acaacgaggt
      601 tgcagcaggc ccttatggcc gctaccagtt cgaaggcacg caacgctata tccagctctt
      661 ggggccggtc ttcacggcca acttgctcaa tgacaccaac tggcgtctcg gtccgctgat
      721 caaatatcgg tctgcccgcg atagtggcgt ggatgacaag gtcgtcagcg aaatggacaa
      781 ggtggacagt gcaatcgagg gtggcgtgtt tgtcggatac aagctggcct tgagtgacac
      841 gcctatgcat caggtcaatt tcaatgccga tttcgaaagc ggcaaaaatg gcgcggaagg
      901 gcatctgagc gcgaactact tccagccatt cagcaagacg atcatcggca acatcggcct
      961 gggtctgact tacggcaacg acaagttcgt ggaaacctat ttcggcgtca ctagtgcgca
     1021 tgatgtctcg ctttacccca gccttggcgg cagaccatac aatacctcca gtggcgtcgt
     1081 tggatataac atcccattcg gcatttccgc attcctgacc gaggaatggc tgctgtcggt
     1141 cggtggtcgt tacgaacggc tggtgggcga tgccaaagac agccccgtgg ttgatcagcg
     1201 cggggatgct aaccagtgga tcggcggtct cggtctggct tacgtcttca agtaaagcga
     1261 ctgatgcggt agtcaaaaaa cggcggcttt gcccgccgtt tttgtttggg gccggattga
     1321 gagaggtcga ttttccgaga tcgggcgcag tcccgggcat ggctttaagc tccgcataca
     1381 aattgccgaa gtcgggtggc tttcgggtaa aatccaaagt tttaaaacca acgaatttcc
     1441 catgctccgt ctccgtggca gtgccgcact ttccccgttt cgtctcgaca agattctggc
     1501 cgcgcttaag gtcgcggcgc cacgcatttc acacctgtat accgagttct ggcacttcgc
     1561 ctgggccgag ggcgaactaa gcgtagacca gcaggacacg ctgcaaaaaa tccttaccta
     1621 tggccccacg atggctgagg aagcacccat tggcgagatg ttcctggtca tcccgcggcc
     1681 cggcaccatt tcgccttggt cgtcgcgcgc caccgatatt gcgcggcact gtggcctgga
     1741 gatgatgcag cggttggagc gcggtgtcgc ctattacgcg gcgaccgccg atggcagccc
     1801 gctgactgaa gaggaaaagc gcgcgctgag gccgctgatc catgaccgca tgacggaagc
     1861 ggtattcccc acgctggatg atgccgcacg cctttaccac actgccgagc cggctccttt
     1921 gtccagtgtg gacatcctag gcggcggcaa ggcggcgttg caagcggcca acagcgacat
     1981 gggcctggcg ctgtcgccgg acgaggtgga ttacttggta gaaaacttca cccgcattgg
     2041 ccgcaatccg accgacgtcg agctgatgat gttcgcccag gccaattccg agcactgccg
     2101 gcacaagatt ttcaacgccg actgggtggt cgacggagtg gagcagtcgc agtcgctgtt
     2161 cgatatgatt cgcaacacgc acaaactgaa cccgggaaaa acggtggtcg cctattctga
     2221 caacgcttcc atcgtgcagg gcgagaagac caggcgtttc tatccgcagc cggacgggcg
     2281 ctacgagtac gtcgaagagg agatgcattt cctgatgaag gtggagacgc acaaccaccc
     2341 gaccgcgatt gcaccttttg ccggcgcggc tacgggtgct ggtggcgaaa tccgcgacga
     2401 aggggcgacc ggcagcggct ccaagcccaa ggccgggttg actggttttt ccgtatccaa
     2461 cctcaacatt cctggcttca agcagccatg ggaagccgat tacggcaagc cggggcgcat
     2521 tgcgtctgcg ctgcagatca tgatggacgg cccgttgggg ggcgctgcct acaacaacga
     2581 attcggccgg cccaacattg ccggctattt ccgcacgcta gaactggcgg cagcggggga
     2641 agtgcgcggc taccacaagc ccatcatgct ggcgggcggc attggcagca tttccgccca
     2701 gcactcgaag aagaatccca tcccgccggg tgcggcgttg atccaactgg gcggaccagc
     2761 catgctgatc ggactgggcg gtggcgcggc ttccagcatg gatactggat ccaatgcaga
     2821 gaacctggat ttcgactcgg tgcagcgcgg caacccggaa ttgcagcggc gcgcgcagga
     2881 agtcatcgac cgttgttggc aactgggaga gcgcaacccc atcctgtcca tccacgacgt
     2941 cggcgccggc gggctgtcaa atgccttccc ggaactggtc aacgatgccg gacgtggcgc
     3001 cgtcttccaa ctgcgtgata tccacaatga agagcctggc atgagcccca gggcggtgtg
     3061 gtgcaacgag gcgcaggagc gctacgtgct ggctgtgtcc aaggatgacc tgtaccgctt
     3121 caaggaaatc tgcgagcgcg agcgctgccc gttcgccatc gtcggcgagg ccaccgaaga
     3181 acgccatctg gcggtggagg acagccactt caacaacaag ccggtggaca tggatcttgc
     3241 cgtgctgctg ggcaagccgc ccaaaatgac gcgtgacgtg gcgcatctga agcgccaact
     3301 gcccgaattt gatgccgcca gcatcgacct ggccgaggcg gtcgaccgcg tgctgcgact
     3361 gccaggcgtg gcagacaaga ctttcctcat caccatcggc gatcgcagcg tgaccggcct
     3421 gattgcgcgc gaccagatgg tcgggccgtg gcaggtaccg gtggccgacg tcgcggtgac
     3481 gctggccggc tatgaaacgt atcgcggcga ggcgttcgcc atcggcgaga aagcgccgct
     3541 ggcgctgatc gacgccccgg cttccggccg catggcgatc ggcgaagcca ttaccaacat
     3601 cgcggcaagc ctcatcgagg acattggtga gatcaagctg tccgccaact ggatggcgcc
     3661 tgccggccat ccgggggagg acgccgcgct gtatgacacg gtcagggcgg ttggcatgga
     3721 actgtgcccg caactcggca tcagcatccc ggtaggcaag gattccatgt cgatgaaaac
     3781 agtgtgggag gagggcgacg agaagaaggc ggtgaccgcg ccgatttcgc tggtggtgac
     3841 cgctttcgca ccgacgccgg atgcgcgcaa gacgctgacg ccgcaactgc gtaccgacct
     3901 cggaccgacc aagctcatcc tcgtcgatct cggtgctggc aggaaccgcc ttggcggttc
     3961 ggcgctggcg caggtgtacg gcgcagtagg cagcgtggcg ccagacgtgg atgatgccgc
     4021 gcggctcaag gcattcttcg ccgcggtgca gcaactgaac cgcgacggca agattctggc
     4081 ttaccacgac cgctctgacg gtggtctagt ggccaccctg tgcgagatgg cgtttgccgg
     4141 ccattgcggc ctggatattg tgctggatgg catcgtcggc aatacctccg tcaatgctgc
     4201 gctgttcagc gaggagctgg gtgcagtgct gcaggtgcgc gccggcgaca tggaaatggt
     4261 ctgtaccagt ctcgatgctg ccgggctggg tggctgcgtg tacgtaatcg gcgccaccag
     4321 tgagggcgag cacgtgcgta tccagcatgc gggacgcgag gtattcggcg agcgccgcgt
     4381 ggtattgcaa cggcgatggt cggaaaccac tttccacatg cagagcctgc gcgacaaccc
     4441 agcctgtgcg cggcaggaat acgaccgatt gttcgacgta cgtgatcccg gcttgcacgc
     4501 gcaactgagt ttcgatctca aggaagacgt tgccgcgccc tacatcaaca ccggccatcg
     4561 tcccgcgatg gcgatcctgc gcgagcaggg cgtcaacggc caggtggaaa tggcggcggc
     4621 gttcgaccgc gccggcttcg agaccttcga cgtgcacatg agcgacatca tcaccggacg
     4681 tgtcagtctg cgcgatttca agggtttcgt tgcctgcggc ggcttttcct acggcgatgt
     4741 gctcggcgca ggcgagggct gggcgaagtc catcctgttc aacccgcgcg ccagtgacga
     4801 gttcgccgcc ttcttccagc gtggtgacag cttcgctctt ggcgtgtgca atggttgcca
     4861 gatgatgagc aacctgcata gcattatccc cggaacggag cattggccgc atttcgtgcg
     4921 caacgggtcg gagcagttcg aggcgcgctt taccatggtc gaagtgctgg attcgccctc
     4981 gctgttcttc ggtggcatgg ccggtagccg tatgccgatt gccgtggcgc atggcgaggg
     5041 ctatgcggaa ttttcttctc aggaagcggt cgaaagggcg ctggctgctc aactggtcag
     5101 cgtgcgttat gtggacaact atggaaaccc gaccgaacgc tacccggcca accccaacgg
     5161 gtcgccgcag ggcattaccg gcctgactac ggtggatgga cgcttcagca tcatgatgcc
     5221 gcatccggag cgggtgttcc gcgcggtgca gcattcctgg catcctgatg ggtggggcga
     5281 ggaggggcca tggttgcgca tgttccgcaa tgcacgaaaa tttgtagctt aatcataatg
     5341 acggagataa aaatgagatt gatccagatg ttctttatgg cagcaatgct gatgttatcc
     5401 agcacggttt ttgcaattac cgatgatgag caggtggagt tgtcctacgc gatcgaaaaa
     5461 ggcgatgccg caacggttaa gaaatttctc gataacggcc tcaacccgga cgataccgcc
     5521 tttgcctgga gctggttgca ggtggctgcc aacaagaatc agctggaaat tgtcaagctc
     5581 atggttgagc gcggcgctaa tgtgaattac aagcacccca tcaccaagat gacctcggtg
     5641 gcattcgctg cttacaatgg caacaaggag atggtgacgt acctgatttc caagggtgca
     5701 gatcccaaca tcaagttgcg cggcggcgtt tccttggtca ggatgtcgcg cgacatggga
     5761 aaccaggaga tggcagattt tctgatgcaa aacggcgcca aggatgacgg gtgcaaggaa
     5821 gagaagtgtt tctgacgtac taacctgcta aagacgggtt gcctgggagg gcgagatgaa
     5881 agcacggttc cggatgttgg tcttgctgtt gttcgccccc ttaccgtcgc tggccgtgag
     5941 tgagggcggc catcagcgtg tgctggcggc ctcctgcgcc gcctgccatg gcaccaacgg
     6001 caacagtgtg ggcggcacgc ccgtgctggc cggactggac cgttcctact tcgtgttgca
     6061 gatgcaggca ttccgtactg gcgagcgcag ctccactgtc atgcatacgc atgccaaagg
     6121 tttgaccgaa gaagaaatcg ggctactggc cgaatacttt tccgtagaaa agcgtacggc
     6181 gtcctcatcc cccaagccgc tgggggatct gtgatgatgc agcgccgcga atttcttaaa
     6241 gttctgggtg ttggcgccat ggccagcgcc gttcccggat tcgccagtgc cgcgggcaag
     6301 gcgcagggac gggtcgtcat tgtcggcgcc ggttatgccg gggcgacggc agccaaatac
     6361 ctgcgcttgt ggggtggcga ccagcttgag atcatcctgg tcgagaaagg tactgccttt
     6421 atttcctgcc cattgagcaa tcttgtgctg ggcggcagca agcgcatcgg cgacctcacc
     6481 ttcaattatt ccggcctgga tcgctacaat atcaagcgtc tttatgcgga agtgagcggt
     6541 atcgatgccg cgcaacgcaa ggtgtacgtg ggggatggcg attttctcga atacgaccgc
     6601 ctgattatcg cgcccggcat cgatttcatg tatgaatcct tacccatgct ggagatcgct
     6661 tcggcgcagg aggtagtgcc gcatgcctgg aaggccgggc cgcagaccgt caatctgcgc
     6721 aaacaactgg aagcgatgcc ggacggcggc gtgtttctgc tctccgttcc caaggcgccc
     6781 taccgttgtc cgcccgggcc gtatgaacgg gtgagccagg tggcgttcta tctcagaaat
     6841 gccaagccga aatcgaagat tatcgtgctg gatgccaacc cggaaatcgt gtccaagaaa
     6901 ggcctgttca ccaaggcatg ggcggagctt taccctggca tggtggatta ccgccccaac
     6961 agccaggtgg tcgaagtcga cgttgcggcg cgcactgtca aaaccgaatt cgagacggtc
     7021 aaggccgacg tgcttaatgt cataccgccg cagcgcgccg gcaggatcgc ccaacaggtg
     7081 ggggcagcca acgtcgaccg gcgctggtgc gaagtggatt tcctgactta cgaatcgaaa
     7141 gcggtccccg gcattcacat tattggggat tcggtttccg ccgccctgcc caagtcggcg
     7201 cacatggcga cttcgcaggc caaggtgtgc gcctcggcga tcaccaccct gctggctggt
     7261 gatacgcccg acccgcaacc ggtgttcgcc aatacctgct acagctttgt cagcgacaag
     7321 ctggcgatgc acgtcgccca tgtctaccgc tacgacccgg ccaaaaagat catgctgccg
     7381 gcggaaggcg gcggcgtatc cgacaggcca accgaactgg agggcgacta cgcccaatgg
     7441 tgggcgaaga atatctggtc cgacgtgctg agctgatgcg ccggctgccc gcgtttgttc
     7501 tgttaaccct gattgcctgt ctggcgcgtg cggatatggc atcggtggtg ccggtgccga
     7561 acgaccctgc cgtcgcgttg caggttccgg ccgatttcag catacaggca tttgcccgcg
     7621 tcaacccggc cggtggcggc tatttccgcg ggccgcgttt catggccttt ggcccggacg
     7681 gaaatctcta cctctccctc gggctcgaca acaaggtggt catgctgccg gatcgcgacc
     7741 atgatggccg ggcggatgaa atagccacgg tggcggacaa actcaacggt ccgcagggac
     7801 tggtattcgt ggaaggcatg ctctatgtcg ccaaccagga tggcgtggtg aggctggagc
     7861 agggcgacgg caaatggcct gcctccgagg tgaaacccat tatcggcaac ttgccaactg
     7921 gcgggcatac gatgaaaagc ctaaagctgg ggccggatgg cttgctctat ctcaatgtcg
     7981 gttccagctg caatgtttgc gaggaaagcg atccattgcg cgccaccctg ttgcgctaca
     8041 gcgtggacgg caagccggca ggggcgttgc cttccgtcgg gcgtcaccct tcctcgccaa
     8101 ttttcgcctc ggggctgcgc aatacgcagg gtttcgcctg gcatccggca acgggcgcgt
     8161 tatacgcaac caataacggg gctgacatgc gttccgacac caagggcggt aagccggtgg
     8221 actacctgcc gcccgaacat cttaacct
//
LOCUS       DAOMFA010000071         8182 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_1031_length_8182_cov_5.410114, whole genome shotgun sequence.
ACCESSION   DAOMFA010000071 DAOMFA010000000
VERSION     DAOMFA010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 8182)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8182)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8182
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1031_length_8182_cov_5.410114"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..675
                     /locus_tag="PKW44_04945"
     CDS             <1..675
                     /locus_tag="PKW44_04945"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HQR50965.1"
                     /translation="LFSVVLSVLAGLVITPLFIGPIRVGTVIATIIIPGITVPPIIWL
                     TMRILYELDLAERKLQILSVTDDLTGIYNRRHFVRAAEMALAYAKRYSENLAMILIDL
                     DLFKNVNDNFGHQSGDQVLIEVVRRTSDCLRASDTFARYGGEEFVVLLPSTDLEGAKS
                     LAERIRMRIGQCPVHVDGKTIPLTISAGVTILEPHDDGIDPLLQRADKALYTAKQGGR
                     DRVEIA"
     gene            complement(676..4563)
                     /gene="hrpA"
                     /locus_tag="PKW44_04950"
     CDS             complement(676..4563)
                     /gene="hrpA"
                     /locus_tag="PKW44_04950"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HQR50966.1"
                     /translation="MTNLLSRQLTTQLEARLVQCLLADRYPLQRKLRQAAEALQAGRP
                     ADRLLAEATTRIDISMQRYAARLAALPKPEFVQDLPVTARKDEIAAAIAKHQVVIVCG
                     ETGSGKTTQLPKICLELGRGVGGLIGHTQPRRIAARSVASRIAQELKSPLGEAVGYKV
                     RFNDRTHDTSYIKLMTDGILLAETQGDRFLNAYDTIIIDEAHERSLNIDFLLGYLKQL
                     LPKRPDLKVIVTSATIDADRFSRHFNGAPVIEVSGRMYPVEVRYRPLQETEEDEQEET
                     MEAAILDAVDDLSRLGGGDILVFLPGEREIRDTAEHLRKHHPRGAEILPLYARLSIED
                     QQKIFRPSGGRRIVLATNVAETSLTVPGIKYVIDTGLARINRYSPRAKVEQLQIEKIS
                     QAAARQRAGRCGRVSNGICVRLYAEEDFNNRPEFTDPEILRSSLASVILRMASLKLGD
                     VDDFPFIEAPHSRLVADGYQLLQELGAVDEQRRLTDIGSQLARLPLDPRVGRMILAAK
                     KEGCLKEILIIGAALAIQDPRERPMDKREAADQAHAKFADERSDFMGFLKLWAFYDEA
                     LKHKKSNKELLTKCHQNFLSFLRLKEWRELHGQLAGIVGEMDFHTNSEDASYDQIHRA
                     LLAGLLGNVGFKDGEADTYLGARGIRFSIAPGSALKKQRPKWVMAAELVETAKLYARC
                     VARLDPDWIEPLARGLTQSHYSDPRWDRKPAQVVAWERVSLYGLTIVPKRRVHYGPID
                     PTESREIFIREALANMEFDTRAPFFGHNQKLITEVEELEHKARRQDVLVDEHALFAFY
                     DARIPAGIHNGITFEKWRKEAEQASPGLLYLTRDDLMRHAASGITEAQFPETFSLDGV
                     EVPLKYRFEPGHPLDGVTATIPLALLNQLDPTKAEWLVPGMLREKITYLVKSLPKTIR
                     RVCVPVPEFVTGSLEQAGIGEGELLPALAAYIQQRTGLKLSASDWDEAIPSHLLMNFR
                     VVDDAGRELAMGRDWVALRNQLGQAAQLTFRNASPDIEKSGLKQWDFGDLPKKLDFSR
                     NGQKMTGYPALEDSGDSVAVKLFDTAAAALESHRRGVRRLMRFELKEQMKQLEKGLPG
                     FNQYALLLRNIMNPDELREDMLTAIADRAFIGEDELPRSNADFMQLKQRARARLPAVV
                     EGAGRLAQAIAAEIQPLMQKLAGLPPPMARLKKEIEEQLARLLPKHFFSITPWERLQH
                     LPRYLKALRLRLEKYPSSIERDSRHAQAVQQLWARWEERLATQRKQGEISDGLADFRW
                     LIEELRVSLFAQELKTPFPVSVKRLEKAWSELS"
     gene            4745..4816
                     /gene="pqqA"
                     /locus_tag="PKW44_04955"
     CDS             4745..4816
                     /gene="pqqA"
                     /locus_tag="PKW44_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013440845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone precursor peptide PqqA"
                     /protein_id="HQR50967.1"
                     /translation="MWTKPEVTEMRFGFEVTMYVCNR"
     gene            complement(4876..5097)
                     /locus_tag="PKW44_04960"
     CDS             complement(4876..5097)
                     /locus_tag="PKW44_04960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR50968.1"
                     /translation="MIVFVAAIVLCACSRHYEPLEDSKKARLVSELINDAPRCKTFKD
                     QLASPSMDDDKIDDVYHAAVKAQCTNRHS"
     gene            complement(5191..5616)
                     /locus_tag="PKW44_04965"
     CDS             complement(5191..5616)
                     /locus_tag="PKW44_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR50969.1"
                     /translation="MMKIALLLTALLAPVATQAQPAFTGPDYSGVYDCTGNDAHEGPY
                     TGTVTMELDAAQSTGSYGAYKFRLDVPGFGAYLGEAVSEGDRIAMYFALQDPTTHDFG
                     TGLATVSRGADGRTSFSKFYYEPEFKGGNYGTEECMLRK"
     gene            complement(5632..6453)
                     /locus_tag="PKW44_04970"
     CDS             complement(5632..6453)
                     /locus_tag="PKW44_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HQR50970.1"
                     /translation="MTLIPEIKPGQSVELLKELHILTRDGKLNQDSRRKLKQVYHLYH
                     FIEPLLNELLAGSENPILADHGAGKSYLGFILYDLFFKQQAGGHIYGIETRPELVEKS
                     RELAQRLGFARMSFLNLSVEESIHSAELPETIDIVTALHACNTATDDAIRFGLHKQAK
                     FLVLVPCCQAEVAEALRRHKNESFGMTPLSELWRHPLHTREFGSQITNVLRCLQLEAH
                     GYQVTVTELVGWEHSMKNELIVARRTGMPRRNAQERLEHILRELNLGELRERFVS"
     gene            6545..7453
                     /gene="ylqF"
                     /locus_tag="PKW44_04975"
     CDS             6545..7453
                     /gene="ylqF"
                     /locus_tag="PKW44_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HQR50971.1"
                     /translation="MAIQWFPGHMTSARKKAAETMEFIDIVVEVLDARLPAASSNPMI
                     EELRLFRQRPCLKILNKADLADPAATQAWLNFYNKQPGVKAVALSCKKPGDAGKVVGL
                     SQSIAPHRGSNLKPLRMMIMGIPNVGKSTLMNALLNRRVAKVGDEPAVTKSQMRIDLN
                     DQVSIIDTPGMMWPKIQYDSDGYMLAASHAIGRNAVIDEDVAIFLADLLLARYPALLA
                     ARYKLDVSGMDGVDVVEAVGRKRGYRLKGGDIDLEKAAMTLLTDYRSGALGRISLETP
                     VTRQAMLAASKPAEPAESEEEPEDVD"
     gene            7446..7856
                     /locus_tag="PKW44_04980"
     CDS             7446..7856
                     /locus_tag="PKW44_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family metal-binding protein"
                     /protein_id="HQR50972.1"
                     /translation="MTKPATPCPCGGKSYATCCAPYHDGTPALNAEALMRSRYSAYVL
                     DLEPYLLATWHPDTRPAAADPKGDGGRWIGLEVKRHTVESADSATVEFVARYKVGGRA
                     QRLHEISRFVREHGRWLYVDGRFPDNQSPTTQRE"
     gene            7860..>8182
                     /locus_tag="PKW44_04985"
     CDS             7860..>8182
                     /locus_tag="PKW44_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 family protein"
                     /protein_id="HQR50973.1"
                     /translation="MAASSLLALIDDIASILDDVALMTKVAAKKTAGVLGDDLALNAQ
                     QVSGVDADRELPVVWAVAKGSLVNKLILVPAALATSALAPWAIVPLLMLGGAYLCFEG
                     FEKLA"
BASE COUNT         1579 a         2529 c         2426 g         1648 t
ORIGIN      
        1 ctgttcagcg ttgtgctgtc ggtgctggcg ggtctggtga tcaccccttt gtttattggt
       61 ccgatcaggg tcggcaccgt catcgccacc atcatcattc ccggaatcac cgtaccgccg
      121 atcatctggc tcaccatgcg catcctgtat gaactggacc tggccgagcg caaactgcaa
      181 atcctctccg taacagacga tctgaccggc atctataatc ggcgacattt tgtcagggcg
      241 gcagaaatgg cgcttgccta tgcaaagcgc tactccgaaa atctggccat gatcctgatc
      301 gatctggatc tcttcaagaa cgtcaatgac aacttcgggc atcagtcggg cgatcaggtg
      361 ctgatcgagg ttgtgcgccg cacttcggac tgcctgcgcg cctcggacac cttcgcacgt
      421 tacggcggag aggagtttgt ggtactgctg cccagcaccg atctcgaggg tgccaagtcg
      481 cttgcggaac gtattcgcat gagaatcgga cagtgcccag tgcatgtcga cggcaaaacc
      541 atccccttga ccatcagcgc gggagtaacc atcctggagc cgcatgacga cgggatagat
      601 ccattgctgc agcgggcgga caaggcgctc tatactgcca agcaaggcgg gcgcgatcgc
      661 gtcgagatcg cctgatcagg acagctctga ccaggctttc tccagtcgct tcaccgacac
      721 cggaaatggc gtcttgagtt cctgggcaaa tagcgacacc cgcagctctt cgatcagcca
      781 acggaaatcc gccagaccat cgctgatttc accttgcttg cgctgtgtcg ccagccgctc
      841 ctcccagcgc gcccacagtt gctgcacggc ctgggcgtgg cggctgtcgc gctcgatact
      901 gctgggatat ttttccagcc tgagccgcag cgccttgaga tagcgcggca ggtgctgcaa
      961 gcgctcccag ggcgtgatgc tgaagaagtg ttttggcagc aggcgtgcga gctgttcctc
     1021 aatctctttt ttcaggcgcg ccatcggcgg cggcaaacca gcaagctttt gcatcagagg
     1081 ctggatctct gcggcgatag cctgcgccag ccgcccggca ccttccacca cggcgggcag
     1141 ccgggctctg gctcgctgct tgagttgcat gaagtcagcg ttgctgcgcg gcaattcgtc
     1201 ttcgccgatg aaggcgcggt cggcaatggc cgtcagcatg tcttcgcgca gttcgtccgg
     1261 attcatgatg ttgcgtagca gcagcgcata ctgattgaaa ccaggcaagc ctttctccag
     1321 ctgcttcatc tgctctttca gttcgaagcg catcagacgg cgtacgcccc ttcggtgcga
     1381 ttccagcgca gcagctgcgg tatcgaacag cttgaccgcc acactgtcac cgctgtcttc
     1441 cagcgcagga tagccggtca ttttctggcc gttgcggctg aagtccagtt tcttcggcag
     1501 gtcgccgaaa tcccactgct tgaggccgga cttctcaatg tccggcgagg cgttacgaaa
     1561 cgtcaactgc gcggcctggc caagctggtt tctgagcgca acccagtcgc gtcccatcgc
     1621 cagttcgcgc ccggcatcgt ccaccacgcg gaagttcatc agcagatggg acgggatcgc
     1681 ctcatcccag tccgaggcgg aaagcttcag ccccgtgcgc tgctggatgt aggcggccag
     1741 tgccggcagt agttcaccct caccgatccc ggcttgctcc agggaaccgg tgacaaactc
     1801 cggcaccggt acgcacacgc ggcggatagt tttcggcagc gatttcacca ggtatgtgat
     1861 cttctcgcgc agcatgcccg gtaccagcca ttcggccttg gttgggtcaa gctggttgag
     1921 cagcgccaac ggaatagtgg cggtcacgcc gtccaggggg tgccctggct cgaagcgata
     1981 tttgagcggc acttccacgc catccagact gaacgtctca gggaattgcg cctcggtgat
     2041 gccggaagcc gcatggcgca tcaagtcatc gcgagtcaga tacagcagtc cgggactagc
     2101 ctgctcagcc tccttccgcc atttctcgaa ggtgatgcca ttgtggatac ccgccgggat
     2161 gcgcgcatcg tagaaggcaa acagcgcgtg ctcgtccacc agtacgtcct gccggcgcgc
     2221 cttgtgctcc agttcctcga cctcggtgat gagtttctgg ttatggccga aaaacggcgc
     2281 gcgggtgtcg aactccatgt tcgccagcgc ttcgcggatg aagatttcgc gcgactcggt
     2341 cgggtcgatt ggcccgtaat gcacgcggcg cttgggcacg atggtgagac cgtacaggct
     2401 gacgcgctcc cacgccacca cctgcgccgg cttgcggtcc cagcgcggat cgctgtaatg
     2461 gctttgcgtc aggccacgcg ccaacggctc gatccagtcc ggatcgagcc tggctacaca
     2521 gcgtgcatac agcttggcgg tttccaccag ttcggccgcc atcacccact tggggcgctg
     2581 cttcttcagc gccgaaccag gcgcaatact gaagcggata ccgcgggcgc cgaggtaggt
     2641 gtcggcttcg ccatccttga agccaacgtt acctagcagg ccggccagca gggcacgatg
     2701 gatctggtcg tagctcgcat cttcgctatt ggtatgaaaa tccatctcgc cgacgatgcc
     2761 agccagctgc ccgtgcaact cgcgccactc tttcagacgc agaaacgaca ggaagttctg
     2821 gtggcatttg gtcagcagtt ccttgttgga cttcttgtgc ttgagcgctt cgtcgtaaaa
     2881 cgcccacagc ttgagaaagc ccatgaaatc ggaacgctcg tcggcgaact tggcatgcgc
     2941 ctgatcagcc gcttcgcgct tgtccatcgg acgttcgcgc ggatcctgga tcgctagcgc
     3001 cgcgccgatg atcaaaatct ccttaagaca gccttccttc ttcgccgcca gaatcatgcg
     3061 gccgacgcgc ggatcgagcg gcagcctggc gagctgcgag ccgatatcgg tcagccggcg
     3121 ctgctcatcc accgcgccca gctcctgcag caactggtag ccgtcggcga ccagccttga
     3181 gtgtggcgcc tcgatgaaag gaaaatcgtc cacgtcgccc agcttgagcg acgccatgcg
     3241 cagaatgacc gaagccagcg aactgcgcag gatttccggg tcggtgaact caggacgatt
     3301 gttgaaatcc tcctcggcat acaaacgcac gcaaatgccg ttggacaccc gaccgcaacg
     3361 cccggctcgt tgccgtgccg ccgcctgcga aatcttctcg atctgcagtt gctcgacctt
     3421 ggcgcgcggg ctatagcggt tgatgcgcgc caagccggta tcgatcacat acttgatgcc
     3481 cgggacggtg agcgaagttt cagcgacatt ggtcgccagc acgatgcgcc gaccgcctga
     3541 tggacgaaaa atcttctgct ggtcttcgat agacaggcga gcgtacaaag gcaaaatttc
     3601 cgcgcccctg ggatggtgtt tcctcaaatg ttcggcggta tcgcgtatct cgcgctcgcc
     3661 gggcaggaac accaggatgt cgccgccgcc gaggcgggac agatcatcca ccgcatccag
     3721 aatcgcggct tccatggttt cttcctgctc gtcttcttcc gtttcctgca acggccggta
     3781 gcgcacttcg accgggtaca tgcggccgga aacctcgatg accggcgcgc cgttgaagtg
     3841 ccgggagaaa cggtcggcat cgatggtggc cgaggtgacg atgaccttga gatcgggccg
     3901 cttgggtaat aactgtttaa ggtagcccag cagaaagtcg atattcaggc tgcgctcgtg
     3961 cgcctcgtcg atgatgatgg tgtcgtaggc attgaggaag cggtcgccct gcgtctcggc
     4021 cagcaggatg ccgtccgtca tcagcttgat gtagctggtg tcgtgggtcc tgtcgttgaa
     4081 acgcaccttg tagccgaccg cctcgcccag cggcgatttc agctcctgcg caatacggga
     4141 agccaccgag cgcgcggcaa tgcggcgcgg ctgcgtgtgg ccgatcaggc cgccgacacc
     4201 acgccccagt tccaggcata tcttcggcag ttgcgtggtc ttgccggagc cggtttcgcc
     4261 acagacgata accacctgat gcttggcgat agctgcggca atctcatctt tgcgcgccgt
     4321 caccggcaaa tcctggacaa actccggttt gggcagtgca gccaggcgtg cggcatagcg
     4381 ctgcatcgaa atatcgatgc gggttgtcgc ctcggccagc agacggtccg ccggcctgcc
     4441 ggcctggagc gcctccgccg cctgccgcaa tttgcgttgc aacgggtaac ggtcagccag
     4501 caggcactgc acgagtctgg cttcgagctg agtggttaat tggcgggaaa ggagattggt
     4561 catcggtgga ggcggatttt atcatgccgc cagcctcgct ccggtgtacc ggatatcatg
     4621 aaaatcacta gtcgcgattc atgaatggac cgaataccag attgaaatgc ttgggtatag
     4681 tgggaaccgt gcaaggaagc gaaagcaacc atgccaaacc gctaaaactt tcaaggagac
     4741 tgccatgtgg accaagccag aagtgactga aatgcgtttc ggttttgaag tgacgatgta
     4801 cgtctgcaac cgctaggacg ggcgtttttt caagggatta aaacgggagc tacggctccc
     4861 gttcgcgttt tcagttcagg aatgcctgtt ggtgcattgc gccttgacgg cggcgtggta
     4921 gacgtcatca atcttgtcat catccattga tggtgaagcc aactgatcct tgaaggtttt
     4981 gcatctggga gcgtcgttta tcagctcgct gaccagcctc gcctttttac tgtcctccag
     5041 cggttcgtaa tgcctggagc aggcgcagag cacaatggcc gcgacgaaaa cgatcatgag
     5101 tttttgcaca tgcgtaaggt acacgcttaa actttcctga aacttacgga ttgataaaga
     5161 ttttgttggt tgcattcccc acacccggcg ctactttctc agcatgcact cttccgtacc
     5221 atagttgccg cccttgaact ccggctcgta gtaaaacttg ctgaaacttg ttcgcccgtc
     5281 cgctccccgg ctcacagtgg caaggcccgt gccgaaatca tgggtggttg gatcctgcag
     5341 ggcaaaatac atggcgatcc tgtcgccttc ggataccgct tcgccaagat aagcgccaaa
     5401 gcccggcaca tccaggcgga atttgtatgc gccgtagctc cccgtgctct gggcggcgtc
     5461 gagttccatc gtcacagtcc cggtatacgg cccttcgtgc gcgtcgttgc cggtacagtc
     5521 atagacgccg ctgtaatccg gacccgtgaa cgccggctgg gcctgggtcg ctacgggagc
     5581 aagcaaggcc gtcagcaaca aggcaatttt catcatcatt tcgttccttg atcagctaac
     5641 aaaacgttcg cgcagttcgc ccagattaag ctctcgcaag atatgctcca gtcgctcctg
     5701 cgcattgcgc cgcggcatgc cggtgcggcg cgcgacgatc agttcatttt tcatcgaatg
     5761 ttcccagcca accagttccg tcaccgtgac ctggtagccg tgtgcttcga gctgcaggca
     5821 cctcagcaca ttggtgatct ggctgccgaa ctcgcgtgta tggaggggat ggcgccagag
     5881 ttccgacagc ggcgtcatgc cgaacgattc gttcttgtgg cggcgcaagg cctcggccac
     5941 ctccgcctga cagcacggca ccagcaccag gaatttggcc tgcttgtgca gcccgaaccg
     6001 gatcgcatcg tcggtagccg tgttgcaggc gtgcagcgcg gtgacgatat cgatggtctc
     6061 cggcagttcc gcactatgaa tggattcctc caccgagaga ttgaggaaac tcatgcgcgc
     6121 gaagcccaac cgctgggcca gttcgcgcga tttctccacc agttccggcc ttgtctcgat
     6181 gccgtagata tggccgccag cctgctgctt gaagaacagg tcgtaaagga tgaaacccag
     6241 gtacgacttg ccggcaccat ggtcagccaa aatggggttc tcgctcccgg ccagcagttc
     6301 gttcagcaac ggctcgatga aatggtagag gtggtagacc tgcttcagct tacggcggct
     6361 atcctggttg agcttgccgt cgcgcgtcag gatgtgcagc tccttgagca gttcgacgga
     6421 ctggccgggc ttgatttctg gaataagggt cacggcatgg ttgaaagctg aaatttttgc
     6481 cattttagcg gctatcggtg ccttgcgtgg gataatgacg cactttcaac tgactgattc
     6541 gtctatggct atccagtggt tccccgggca catgacctcg gcgcgcaaga aagcggccga
     6601 gaccatggaa ttcatcgaca tcgtggtcga ggtactggac gcgcgcctgc cggcggccag
     6661 cagcaacccc atgatcgagg agctgcgcct gttccgccag cgaccctgcc tgaaaatcct
     6721 caacaaggcc gacctcgccg accccgccgc cacccaggcc tggctcaatt tctacaacaa
     6781 gcagccggga gtgaaggcgg tcgcgttgtc gtgcaagaag ccgggcgatg ccggcaaggt
     6841 ggtcggcctg agccaatcca tcgccccgca tcgcggcagc aacctcaagc cgctgcgcat
     6901 gatgatcatg ggcatcccca acgtcggcaa atccacgctg atgaacgccc tgctcaatcg
     6961 tcgcgtggcc aaggtcggcg acgaaccggc cgtgaccaag agccagatgc gcatcgacct
     7021 caacgaccag gtcagcatca tcgacacgcc aggcatgatg tggcccaaga tccagtacga
     7081 cagcgatggc tacatgctcg ccgccagcca cgccatcggc cgcaacgcgg taatcgacga
     7141 ggatgtcgcc atcttcctgg ccgacctgct gctggcgcgc taccccgccc tgctcgccgc
     7201 acgctacaag ctcgatgtca gcggcatgga tggcgtggac gtggtcgagg ccgtcggcag
     7261 gaagcgcggc tatcgcctca agggcggcga cattgacctg gagaaagccg caatgacctt
     7321 gttgaccgac taccgctccg gcgcgctcgg ccgcatcagc ctggaaacgc ctgttacccg
     7381 gcaggcaatg ctggccgcct ccaaaccggc agagccggca gaaagcgaag aagaaccgga
     7441 agacgttgac taagcctgcc accccctgcc cctgcggcgg caaatcctac gccacctgct
     7501 gcgcgcccta ccacgacggc acacccgctc tgaatgccga ggccctgatg cgttcgcgct
     7561 acagcgccta cgtgctcgat ttggaaccct atctgctcgc cacttggcac ccggatacgc
     7621 gcccggcagc ggctgatccc aaaggtgacg gcggccgctg gatcggcctg gaagtcaagc
     7681 ggcatacggt ggaatcggcc gacagcgcca cggtggagtt cgtcgcccgc tacaaggtcg
     7741 gcggccgcgc ccagcggctg cacgaaatca gtcgtttcgt gcgcgagcac gggcgctggc
     7801 tatacgtgga cggccgcttc cccgataatc agtccccaac aacccaacgg gagtaagttt
     7861 tggccgcaag cagcctgcta gccctgatcg acgacatcgc cagcatcctg gacgacgtgg
     7921 cgctgatgac caaggtcgcc gccaagaaaa ccgccggcgt actgggcgac gaccttgcgc
     7981 tgaacgcgca gcaggtctcc ggggtcgatg ccgaccgcga actgccggta gtgtgggcgg
     8041 tggccaaggg ctcgctggtg aacaagctga tcctggtgcc ggcagcgctg gccaccagcg
     8101 cgcttgcacc ctgggcgatc gtcccgctgc tgatgctggg cggggcctat ctgtgcttcg
     8161 aaggctttga gaagctggcg ca
//
LOCUS       DAOMFA010000072         8105 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_1048_length_8105_cov_4.700373, whole genome shotgun sequence.
ACCESSION   DAOMFA010000072 DAOMFA010000000
VERSION     DAOMFA010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 8105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8105
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1048_length_8105_cov_4.700373"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(83..973)
                     /locus_tag="PKW44_04990"
     CDS             complement(83..973)
                     /locus_tag="PKW44_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydromethanopterin dehydrogenase"
                     /protein_id="HQR50974.1"
                     /translation="MEKPTILHFITPAKNVSPFDINMATDAGYNTIASYTNVLPNEVM
                     GLVQDAIFSRGPRGVKRTGMLIGGRDIDVAMDMLDTAKKSMVPPFEISVFADPSGAFT
                     TAAGMMAAVERQLKSRFGGDLSGRKVSIFGATGPVGGCTAVIAAKFGATVELVAHRSL
                     ADVQVKTEVYNKRYGTSIGYTDGSSDELKKNILHSTDVALCAAAAGVRVITLAQMAGS
                     PTLKVVADVNAVPPTGAEGVDVMADGVAIPGTNAVGVGALAIGNIKYQTQHNLFKKML
                     ETEKPVYLDFLAAFELARNL"
     gene            complement(983..1993)
                     /locus_tag="PKW44_04995"
     CDS             complement(983..1993)
                     /locus_tag="PKW44_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHMP kinase"
                     /protein_id="HQR50975.1"
                     /translation="MPGLPSLVKVATTARLHMGFLDLNGGLGRRFGSIGLALDQPATS
                     LKAWRADGFAATGPGAQRALACARDFAERAGLSGGAHLKLEEAIPEHAGLGSGTQLAL
                     AVGVALARLYGLPLSAREIAAVTARGARSGIGIGAFEQGGLLLDGGRGAQTVVPPLVA
                     RMDFPESWCVLLIFDQSATGVHGGHEIEAFRTLPEFPPGVSADLCRRVLMQALPAVVE
                     QNLPAFGEAIHEIQCRVGDHFAAVQGGGRYTSAAVSSVLQWLREQGVVCVGQSSWGPT
                     GFAVLENESVALALQKKLETRYAGHGQLSFMQCRARNQGSEVQQFYEDGSVHAEHTNS
                     HC"
     gene            2183..3463
                     /locus_tag="PKW44_05000"
     CDS             2183..3463
                     /locus_tag="PKW44_05000"
                     /EC_number="1.2.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran dehydrogenase subunit B"
                     /protein_id="HQR50976.1"
                     /translation="MENFAQPGTVESVTCPFCGLLCDDLAVDRDAAGNLKVSKNGCQK
                     SVTFFGRPPQTPSPRIAGKPANLDAAIARAAGILGNARLPLIAGLGTEVQGVRAVMSL
                     ADKAGAVIDHMNSNGFMRNIQVIQNSGWQITTLAEVRNRVDLLVIVGTDIVSVFPRFF
                     ERQVWNKESMFGQDTSTREVVYLGGRNIDTAAGVAPDGRQPGYLPCDLEQLPDVIAAL
                     RALLLGKKLHAADVAGITAADLAKLAGRLQAAKYSVVAWAAAPLNFTHAELTVQNITE
                     LVKELNKTTRSNGLPLGGIEGDMNANQVSTWISGYPMRTSYARTYPEHDPYHFSTDRL
                     LASGEADALLWVSSFNPERTPPKTGLPTVVFGHADMQFAQEPDVFIPVGMPGADHKGI
                     MFRTDNVVSLPLEQLRNSALPSLSEVLTAIEQAL"
     gene            3465..5141
                     /locus_tag="PKW44_05005"
     CDS             3465..5141
                     /locus_tag="PKW44_05005"
                     /EC_number="1.2.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran dehydrogenase subunit A"
                     /protein_id="HQR50977.1"
                     /translation="MLIKLTGGKVYDPAHNIDGQVRDIYVRDGRIVERPRDGERVDQE
                     YHLSGKVVMAGAIDMHTHIGGGKVNIARTLLPEDHRQDPVPHSAICRAGCGHAVPSTL
                     TTGYRYAEMGYTAGFEPAVLPMNARQAHLEMGDVPIIDKGGYVMLGSDDQLLRMLAAK
                     KDQNAINDYVAWTIHASQAMGIKVVNAGGINAFKFNQRMLDLDEQNSYYQVTPREVLK
                     TLARAVHELNVPHPLHVHGNNLGVPGNIETTLNTMAGVEGYPIHLTHIQFHSYGTEGD
                     RKFSSGAAQIAEAINKRKNVSVDVGQILFGQTVTASGDNMAQYRNHAAGSPHKWVVMD
                     IECDAGCGVVPFKYKDQNFVNALQWAIGLEIFLLVDDPWRIFLTTDHPNGAPFTSYPH
                     LIKLLTNKTFRNEVFATINPDAQKMSTLASIDREYSLYEIAIMTRAGAAKLVGLHDRG
                     HLGVGAAADITVYTDDADREKMFAKPDYVFKDGELVVKDGSVVKVTWGATHTVRPEFD
                     RAIEKEINKYYDTYHTLKADHVKVTDQHIADDGRGRVQIHACQGRREKTA"
     gene            5138..6046
                     /gene="fhcD"
                     /locus_tag="PKW44_05010"
     CDS             5138..6046
                     /gene="fhcD"
                     /locus_tag="PKW44_05010"
                     /EC_number="2.3.1.101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran--tetrahydromethanopterin
                     N-formyltransferase"
                     /protein_id="HQR50978.1"
                     /translation="MIINGVTIDDTFAEAFGMRGTRVIITAQNHKWANTAAAAMTGFA
                     TSVIACGVEAGIECELDPSETPDGRPGVSILMFAMGSAVLMKQLETRMGQCVLTCPTT
                     AAFSGIDEGYPEITRIALCKNLRFFGDGFQISKQFGGKRYWRVPVMDGEFLGEETTGM
                     VRAVGGGNFLILAESQPQALAACEAAIAAMKQVPNVIMPFPGGVVRSGSKVGSKYKSL
                     IASTNDAFCPTLKGITKTDLSPEIESVMEIVIDGLSADDINLAMRAGIAAACELGTAN
                     GIKRISAGNYGGKLGPYHFKLREIMA"
     gene            6043..6849
                     /locus_tag="PKW44_05015"
     CDS             6043..6849
                     /locus_tag="PKW44_05015"
                     /EC_number="1.2.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019918601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018493 - formylmethanofuran dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran dehydrogenase subunit C"
                     /protein_id="HQR50979.1"
                     /translation="MSALALTLKTQPQQRVDCSPLTPDNLAGKTVAEIAAIELQCGKH
                     KLRADAVFDITGTDAANIVIRNSSSKLDYIGHGIKSGRITIHGSAGSYLGFQMKKGEI
                     VLHGNADAYAASGMAGGTLHIHGNVGDFLAAAIPGDRKGMKGGLVIITGNAGDRVGDQ
                     MRRGIVLIEGNAGAYCASRMLAGTIGVMGSVGDYVGFGMRRGTLLLFSTPKLHSTIQD
                     CGSHTLPFINLMFKSFRGLPSQFARLDQNRVRRYAGDIANDGKGEILIFA"
     gene            6880..>8105
                     /locus_tag="PKW44_05020"
     CDS             6880..>8105
                     /locus_tag="PKW44_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018229405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HQR50980.1"
                     /translation="MSEQVIKIDEINKKRGKPKGRTVDPAAVAEIKALLGAESRAPDL
                     LIEHLHKIQDKFSYISSRHIVALAAEMKLSQTEVYEVATFYHHFDVIKEGQIPPPPLT
                     VRVCESLSCDMAGAHGLIDSLKKALGGNVRVQPVPCVGRCQNAPVAVVGQNPIDHAEP
                     QAVLKAVQAKATEAPLTDYISYEQYKAEDGYRLYQDCIAGKRSAEELMGIMENSGLRG
                     LGGAGFPVGRKWRIVSGQPEPRLMAVNIDEGEPGTFKDRHYLELDPHRFLEGMLIAAW
                     AVGINKIYIYLRDEYAGCRKILQREIRNLQNDRPAPLPEIHLRRGAGAYICGEESAMI
                     ESIEGKRGMPRLRPPFVAQVGLFGRPTLEHNMESVFWVRDIVEKGPEWFAAHGRHERK
                     GLRSFSVSGRVKKPGV"
BASE COUNT         1660 a         2661 c         2410 g         1374 t
ORIGIN      
        1 atccgcggca accacccggt agccggccgc agcggccgcc cggacgaacg ggcgggcact
       61 ggcggcggcg atgacaagag gtttagagat tgcgcgccaa ctcgaatgcg gccaggaaat
      121 cgaggtacac cggcttctcg gtttccagca tcttcttgaa caggttgtgc tgggtctggt
      181 acttgatatt gccgatggcc agcgcgccca cgcccaccgc attggtgccg gggatggcga
      241 cgccgtcggc catcacgtcc acgccctcgg cgccggtcgg tggcacggcg ttgacgtcgg
      301 ccaccacctt gagcgtcggc gaaccagcca tctgcgccag ggtgatgacg cgcacgccgg
      361 cagcggcggc acacagggcc acgtctgtgc tgtgcaggat gttcttcttc aattcgtcgc
      421 tgctgccgtc ggtgtagccg atactggtac cgtagcgctt gttgtagacc tcggtcttga
      481 cttgcacgtc agccaacgaa cggtgcgcca ccagttccac cgtggcgccg aacttggcgg
      541 cgatcaccgc ggtacagccg cccaccgggc cggtggcgcc gaagatgctg accttacggc
      601 cggacaggtc gccgccgaag cggcttttga gctgacgttc caccgccgcc atcatgcccg
      661 cggccgtggt gaatgcgccg gaaggatcgg cgaacacgga aatctcgaac ggcggcacca
      721 tggacttctt ggcggtgtcc agcatgtcca tcgccacatc gatgtcacgc ccgccgatca
      781 gcatgccggt gcgtttgacc ccgcgcggcc cgcgcgaaaa aatggcgtcc tgcaccagcc
      841 ccatcacctc gttcggcagc acgttggtgt agctggcgat ggtgttgtag ccagcgtcgg
      901 tggccatgtt gatgtcgaac gggctgacgt tcttggccgg cgtgatgaaa tgaagaatgg
      961 ttggcttttc catgagttac ccttaacaat gtgaatttgt atgctccgca tgcacggagc
     1021 catcctcgta aaactgctgc acttcgctgc cctgattgcg cgcccggcac tgcatgaagc
     1081 ttaattgccc gtgtccggcg tatctggttt ccagcttctt ttgcaaagcc agtgccacac
     1141 tttcgttttc cagtacggcg aaccccgtcg gcccccagga actctggccg acgcagacca
     1201 cgccttgctc ccgcaaccat tgcagcacgc tgctcacggc cgcgctggta tagcgccctc
     1261 cgccctgcac cgcggcgaaa tggtcgccca cgcggcactg gatttcgtgg atcgcttcgc
     1321 cgaacgccgg caaattctgc tcgaccacgg ccggcagcgc ctgcatcaat acccggcggc
     1381 acaaatcggc agacaccccg gggggaaact ccggcaaggt gcggaaagcc tcgatctcgt
     1441 gaccaccatg cacgcctgtc gcgctctggt cgaagatcag cagcacgcac cagctttctg
     1501 gaaaatccat gcgtgccacc agcggcggca ccacggtctg cgcgccacgt ccgccatcca
     1561 gaagcagtcc accctgctcg aaggcgccga taccgatgcc ggaacgcgcg ccgcgcgccg
     1621 tcacagcagc aatttcccgg gccgacagcg gcaagccgta caggcgtgcc agcgccacgc
     1681 ccaccgccag cgccaactgg gtgccggaac ctaatccggc atgttcggga atggcttcct
     1741 ccagcttcag gtgcgcaccg ccggacaatc ccgcccgttc ggcaaaatcc ctggcacagg
     1801 cgagcgcgcg ctgcgcgccc gggcccgtgg cagcgaaacc gtcggcacgc caagctttca
     1861 ggctcgtcgc aggctggtcc agcgccaggc cgatgctgcc gaagcgtcgc cccaaaccgc
     1921 cgttcaggtc cagaaagccc atgtgcaggc gcgccgtggt cgcgactttg acaaggctag
     1981 gcaagcccgg catggttaat ccattcatga ggcgggcatt ttacccgaaa tcggacgtca
     2041 ttcgggacgg tgatacaccc aaaatttatc gcgcaccggc ttcgcaggag tgcttaatcc
     2101 cctgtaaaat taaccttcga aaccaggcga tgcccaaccg ttggcatgat ccgcctgaat
     2161 taaaactgga aacgagattg ggatggagaa ttttgcacag cctggcacgg ttgaatcggt
     2221 gacctgtccg ttttgcggcc tgctttgcga tgacctggca gtcgaccgcg atgccgcggg
     2281 caacctcaag gtcagcaaga acggctgcca gaaaagcgtc actttctttg gtcgcccgcc
     2341 gcaaaccccc tctccacgta tcgccggaaa gccagccaat cttgatgccg ccattgccag
     2401 ggctgccggc atcctcggca acgccaggct gccgctgatc gccggcctcg gtaccgaggt
     2461 gcagggcgtg cgcgccgtga tgagcctggc cgacaaggcc ggcgcagtaa tcgaccacat
     2521 gaactccaac ggcttcatgc gcaacatcca ggtcatccag aattcgggct ggcagatcac
     2581 cacgctggcc gaggtgcgca accgcgtcga cctgctggtc atcgtcggca ccgatatcgt
     2641 cagcgtgttc ccgcgcttct tcgagcggca ggtgtggaac aaggaatcca tgttcggcca
     2701 ggacacttcc acgcgcgaag tggtctatct gggcggacgc aacatcgaca ccgcagccgg
     2761 cgtcgccccc gacggccgcc aacccggcta cctgccgtgc gatctggaac agttgccgga
     2821 tgtcatcgcc gcgctacgcg ccttgctgct gggcaagaag ctgcacgccg ccgatgtggc
     2881 cgggattacc gctgccgacc tggcaaaact ggccgggcgc ctccaggccg ccaaatacag
     2941 cgtggtcgcc tgggccgccg ccccgctcaa tttcacccat gccgagctga cggtgcagaa
     3001 catcacggaa ctggtcaagg aactgaacaa gaccacccgc tccaatgggc tgccgctggg
     3061 cggcatcgag ggcgacatga acgcgaacca ggtcagtacc tggatttccg gctacccgat
     3121 gcgcacttcc tatgcacgca cctacccgga gcacgaccct taccattttt ccaccgaccg
     3181 cctgctggcg agcggcgaag cggatgcgct gttgtgggta tcgtccttca acccggaacg
     3241 cacgccgccc aagacaggcc tgccgacggt ggtgttcggc cacgccgaca tgcaattcgc
     3301 acaggagccg gacgtgttca tcccggtagg catgcccggc gccgaccaca agggcatcat
     3361 gttccgcacc gacaacgtgg tctcgctgcc gctggaacaa ctgcgcaata gcgctttacc
     3421 aagcctgagc gaagtgctca cggccattga acaggcgctc tgacatgctg atcaaactca
     3481 caggcggcaa agtttacgac cccgcgcaca acatcgacgg ccaggtgcgc gacatttacg
     3541 tgcgtgacgg ccgcatcgtc gagcgtccgc gcgatggcga acgcgtcgac caagaatatc
     3601 acctgtccgg caaggtggta atggcaggcg ccatcgacat gcacacccat atcggtggcg
     3661 gcaaggtcaa catcgcccgc accctgctgc cggaagacca ccgccaggat ccggtaccgc
     3721 acagcgccat ctgccgtgcc ggctgcggtc atgccgtgcc ttccaccctg actaccggct
     3781 accgctatgc cgagatgggc tacaccgccg gcttcgagcc cgccgtgctg ccgatgaatg
     3841 cgaggcaggc ccatttggaa atgggcgacg tgcccatcat cgacaagggc ggctacgtca
     3901 tgctgggcag cgacgaccag ttgctgcgca tgctggccgc aaagaaggac cagaacgcca
     3961 tcaacgacta tgtggcatgg acgattcatg ccagccaggc catgggcatc aaggtggtca
     4021 acgccggcgg catcaacgcc ttcaagttca accagcgcat gctggacctc gacgagcaga
     4081 attcgtacta ccaggtgacc ccgcgcgaag tgctgaagac gttggcccgc gcggtgcatg
     4141 aactcaacgt gccgcacccg ctgcacgtgc atggcaacaa cctgggcgtg ccaggcaata
     4201 tcgaaaccac gctcaacacc atggcgggcg tggaaggcta ccccatccac ctcacccaca
     4261 tccagttcca cagctacggc accgagggcg accgcaagtt ttcttccggc gcggcacaga
     4321 tcgccgaggc catcaacaag cgcaagaacg tgtcggtcga cgtcggccag atcctgttcg
     4381 gccagaccgt caccgcctcc ggcgacaaca tggcgcaata tcgcaaccat gccgccggca
     4441 gcccgcacaa gtgggtggtg atggacatcg agtgcgacgc cggctgcggc gtggtgccat
     4501 tcaagtacaa ggaccagaat ttcgtcaacg cgctgcagtg ggcgatcggc ctggaaatct
     4561 tcctgctggt ggacgacccg tggcgcatct tcctcaccac cgaccaccca aacggcgcac
     4621 ccttcaccag ctacccgcac cttatcaagc tgctgaccaa caaaaccttc cgcaatgaag
     4681 tgttcgccac catcaacccg gatgcgcaga agatgagcac gctggcctcg atagaccgcg
     4741 aatactctct gtatgaaatc gccatcatga cccgcgccgg cgccgccaag ctggtcggcc
     4801 tgcatgaccg cggccacctg ggcgtgggcg ccgccgccga tatcaccgtg tacaccgacg
     4861 atgccgaccg cgaaaaaatg ttcgccaagc cggattacgt gttcaaggac ggcgaactgg
     4921 tggtcaagga cggcagcgtg gtcaaggtta cctggggcgc gacgcatacc gtccgcccgg
     4981 aattcgaccg cgccatcgaa aaggaaatca acaagtatta cgacacctac cacacgctca
     5041 aggccgacca cgtcaaggtc actgaccagc acattgccga cgacgggcgc ggacgggtgc
     5101 agattcacgc atgccagggc cgcagggaga agacggcatg atcattaatg gcgtcaccat
     5161 cgacgatact ttcgccgaag ccttcggtat gcgcggcacg cgcgtcatca tcactgcgca
     5221 gaatcacaag tgggccaata ctgccgctgc agccatgact ggctttgcca cctccgtcat
     5281 cgcctgcggc gtggaagccg gcatcgagtg cgaactcgac ccctccgaga cccccgatgg
     5341 ccgccccggc gtttccatcc tgatgttcgc gatgggcagc gcggtgctga tgaagcagct
     5401 ggagacccgc atggggcaat gcgtgctgac ctgccccacc acggcggcgt tctccggcat
     5461 cgacgagggc tacccggaga tcacccgcat cgccttgtgc aaaaacctgc gcttctttgg
     5521 cgacggcttt cagatttcca agcagttcgg cggcaagcgc tactggcgcg taccagtaat
     5581 ggacggcgaa ttccttggcg aagaaactac cggcatggtg cgcgcggtgg gcggcggcaa
     5641 tttcctcatc ctcgccgagt cgcagccgca ggcgctggcg gcttgcgaag cggcgatcgc
     5701 agcgatgaag caagtaccca acgtcatcat gccattccca ggtggcgtgg tgcgctccgg
     5761 ctccaaggtc ggctccaaat acaagtcgct gatcgcttcc accaacgacg ccttctgccc
     5821 gacgctgaag ggcattacga aaacggattt gtcgccggag atcgaatcgg tgatggaaat
     5881 cgtcatcgac ggcctgagcg cggacgacat caacctggcc atgcgcgccg gcatcgccgc
     5941 cgcctgcgaa ctggggacgg ccaacggcat caaacgcata tccgccggca attatggcgg
     6001 caaactgggg ccataccact tcaaactacg ggagatcatg gcatgagcgc cttggccctg
     6061 accctcaaaa cccaaccgca gcagcgcgtg gactgttcgc cgctgacgcc ggacaacctg
     6121 gctggcaaga ccgttgccga aatcgctgcc atcgaattgc agtgcggcaa gcataaactg
     6181 cgtgccgatg ccgtgttcga catcaccggc accgatgctg ccaatatcgt catccgcaac
     6241 agtagcagca agctggacta tatcggccac ggcatcaaga gcggcagaat tacgatccac
     6301 ggcagcgccg gttcctacct cggcttccag atgaagaagg gcgagatcgt tctgcacggc
     6361 aacgccgacg cctatgccgc cagcggcatg gcgggtggca cgctgcacat tcacggcaat
     6421 gtgggcgact tcctcgcagc cgcgatcccc ggcgaccgca agggcatgaa aggcggcctc
     6481 gtgatcatca ccggcaacgc aggggaccgc gtcggcgacc agatgcggcg cggcatcgtg
     6541 ctgatcgaag gcaatgccgg cgcctattgc gcctcgcgca tgttggccgg taccatcggc
     6601 gtgatgggtt cggtcgggga ttacgtcggg ttcggcatgc gccgcggcac gctgttattg
     6661 ttcagcacgc ccaagttgca ttcgaccatt caggattgcg gcagccatac gctgcccttc
     6721 atcaacctga tgttcaaatc gttccggggt cttccgagcc agtttgccag acttgaccag
     6781 aacagggtac ggcgttatgc cggggatatt gccaacgatg gcaagggcga aattctgatt
     6841 ttcgcctgat tgcaaagctg aaaggcacgg aggaaaggca tgtcggagca ggtaatcaag
     6901 attgatgaaa tcaacaaaaa gcgcggcaag cccaaggggc gcaccgtcga tcctgccgcc
     6961 gtggccgaga tcaaagcctt gctcggcgcc gagtcgcgtg cacccgacct gctgatcgaa
     7021 cacctgcaca aaatccagga caagttcagc tacatttcct ccaggcacat cgtcgcgctg
     7081 gctgccgaaa tgaagctgtc gcagaccgaa gtctatgaag tcgccacctt ctatcaccac
     7141 ttcgacgtga tcaaggaagg ccagatcccg cccccaccat tgaccgtcag ggtgtgcgaa
     7201 tccctgagct gcgacatggc aggcgcgcat ggcctgatcg attcactgaa aaaagcactg
     7261 ggtggcaacg tgcgcgtaca gccggtacct tgcgtcggcc gctgccagaa cgcccccgtg
     7321 gcagtagtcg ggcagaatcc catcgaccac gccgaaccgc aagccgtact caaggcggtg
     7381 caagccaagg cgacggaagc cccccttacc gactacatct cctacgaaca atacaaggca
     7441 gaggacggct accggctcta ccaggactgt atcgccggca aacgctctgc agaagagctg
     7501 atgggcatca tggaaaactc cggcctgcgc ggcttgggcg gcgccggttt cccggtcggg
     7561 cgtaaatggc gcattgtcag cggccagccg gagccgcgcc tgatggcggt gaatatcgac
     7621 gagggcgaac ccggaacttt caaggacagg cactacctgg aacttgatcc gcaccgcttc
     7681 ctggaaggca tgctgatcgc cgcctgggcg gtcgggatca acaagatcta catctacctg
     7741 cgcgacgaat acgccggctg ccgcaagatt ctgcagcgcg agatcaggaa tctgcaaaac
     7801 gaccgccctg ccccgctgcc ggaaatccat ctgcggcgcg gtgccggtgc ctatatctgc
     7861 ggcgaggaat cggcgatgat cgagtccatc gagggcaagc gtggcatgcc acgtctgcgc
     7921 ccgccgttcg tcgcgcaggt cggcctgttc ggtcgcccca cgctggaaca caacatggag
     7981 tcggtgttct gggtgcgcga catcgttgaa aaaggcccgg aatggttcgc cgcccacggc
     8041 cgccacgagc gcaagggcct gcgctcgttc tcggtcagcg gccgggtgaa gaagccgggg
     8101 gtaca
//
LOCUS       DAOMFA010000073         7855 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_1092_length_7855_cov_4.806154, whole genome shotgun sequence.
ACCESSION   DAOMFA010000073 DAOMFA010000000
VERSION     DAOMFA010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 7855)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7855)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7855
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1092_length_7855_cov_4.806154"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..638
                     /locus_tag="PKW44_05025"
     CDS             <1..638
                     /locus_tag="PKW44_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HQR50981.1"
                     /translation="LAGTEVSALLGRMPSAVGYQPTLADEMGKLQERITSTKVGSITS
                     IQAVYVPADDLTDPSPATTFQHLDSTVVLSRDIAALGIYPAVDPLDSTSRQLDPLVVG
                     EEHYNVARSVQQTLQRYKELRDIIAILGMDELSPEDKLAVARARKIQRFLSQPFHVAE
                     VFTGSPGKYVTLKDTIAGFKAIVNGDYDHLPEQAFYMVGSIEEAVEKAKTL"
     gene            662..1093
                     /locus_tag="PKW44_05030"
     CDS             662..1093
                     /locus_tag="PKW44_05030"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HQR50982.1"
                     /translation="MAMTIHLDVVSVEESIFSGLAEFVIAPAVMGEVGIYPFHAPLIT
                     RIKPGAVRVKQPDVEEETVIYVSGGLLEVQPGVITVLADTAIRGHDLDEAMALDSKRK
                     AEEAMRVKGAQIDYAKAQAELIEATAQLRAIQKLRKGGGQH"
     gene            1160..2533
                     /gene="glmU"
                     /locus_tag="PKW44_05035"
     CDS             1160..2533
                     /gene="glmU"
                     /locus_tag="PKW44_05035"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019896786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HQR50983.1"
                     /translation="MQTSKLNVVILAAGKGTRMVSSLPKVLHQVAGSSLLSHVIACAE
                     QLLADNIMVVYGYGGETVRQAIPQDNITWVSQAEQLGTGHAVQQAMPHLSADGVCLIL
                     LGDVPLITADTCRSLLRKAEQGLALLTVEKPDPTGYGRILRGFDGHVQAIVEEKDATP
                     EQRAVREVNSGIMAMPIQLLKDWLGRLDNRNAQGEFYLTDIVGMAVQQGVKVQSELAE
                     DEWEVAGINSKLDLAAVERVYQRSHARRLMQAGVTLRDPERLDVRGELTTGHDVEIDV
                     GCVFEGKVQLGDNVSIGPYCVIKDAVLATGSVIAAYSHIDSAQVGENSRIGPYARLRP
                     GTELMADTHIGNFVEIKNSQVGQGSKINHLSYVGDSTVGKQVNIGAGTITCNYDGVNK
                     YRTVIGDCAFIGSDTQLVAPVTVEAGATIGAGSTITRDAPADTLTLSRSKQITVAGWR
                     RPQKKAK"
     gene            2539..4368
                     /gene="glmS"
                     /locus_tag="PKW44_05040"
     CDS             2539..4368
                     /gene="glmS"
                     /locus_tag="PKW44_05040"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HQR50984.1"
                     /translation="MCGIVGAVAQRNVVPILIEGLSRLEYRGYDSAGIAVLDGGEIRR
                     VRAIGRVAGMQQKAQAEKLDGRLGIGHTRWATHGGVTEANAHPHVSCGEIAVVHNGII
                     ENHDEQRNRLRALGYVFDSQTDTEVIAHLIHYYTRQGNNLLDATQHAVRELVGAYAIG
                     VISLLEPHHLVCARMGCPLLIGLGESENFIASDVSAVLSVTRRVIYLEDGDVADLSSD
                     AVNVFDQTGNPVQRRVRVSDVSLASMELGPYAHFMQKEIHEQPKAISDTIDKILEDRF
                     SPTLFGDDAEQRLGSIEGVKILACGTSYYAGLVAAYWLEAIAGIQTSVEIASEYRYRK
                     AIANPRHLIVTLSQSGETLDTMEALKYAIELGQHQTLSICNVQESAIPRASSMVFYTR
                     AGAEIGVASTKAFTTQLVALFTLCITLAKLRGRITPEQEEQFLEDLRHLPGNVQYALN
                     LEPQIREWAKVFAGKHHALFLGRGVHYPIALEGALKLKEISYIHAEAYPAGELKHGPL
                     ALVDNEMPVVVIAPNDALLEKVKSNMQEVSARGGELFVFADLYSNFGEQQGIHVIRAP
                     RHVGVLSPVVHAIPVQLLAYHTALLKGTDVDKPRNLAKSVTVE"
     gene            complement(4422..4670)
                     /locus_tag="PKW44_05045"
     CDS             complement(4422..4670)
                     /locus_tag="PKW44_05045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR50985.1"
                     /translation="MQPRTLMFFLASLLISSCSGPYVMEEVNVQDGVVVGEKEVDVGA
                     NGQITGESEEGYVPNDRYLDDYQDGGQNLGPNAPGYVP"
     gene            4799..5128
                     /locus_tag="PKW44_05050"
     CDS             4799..5128
                     /locus_tag="PKW44_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HQR50986.1"
                     /translation="MTDTSADDKNIAVLTHLGGIFFSIFPGLIVWLLKKDDNAFIGEQ
                     AREALNFQITVLIGYLIAGLLILVLVGILLWPVIYVANIILCIIAAVRASKGEYYRYP
                     LTLRLIN"
     gene            5143..7188
                     /locus_tag="PKW44_05055"
     CDS             5143..7188
                     /locus_tag="PKW44_05055"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HQR50987.1"
                     /translation="MQNPLLHFSGLPHFDQVKPEHVTPAIEQLLTEGRALVEQLVAAS
                     EAPSWDNFARPLEDMEERISRAWSQVSHMNSVVNSPELREAYNANLPKLTAFYADLSQ
                     HDGLYARFRALRADAGFEQLSPAQRKIVENELRDFRLGGAELPEDQKARFKAIQEELS
                     TLSAKFEENLLDTTNDFSLLVENAAELAGIPADALQAAREAAEADSKPGWKFTLHFPS
                     YMPVLQYADNRALREKLYHAYATRASEFGKQEWDNTALITQILKLRLEAAQLLGFDDY
                     AQLSLATKMAQASAEVMDFLRTLGLRGKPFAEKDMLELRAHAADQLGLAQLEAWDIAY
                     ASEKLREEKYAFSDQEVKQYFPELQVLHGLFKVTETIFGVQVRKIQAPVWHQDVSFYE
                     ITDRNGRLVGQFYLDLYARNGKRGGAWMDEAITRRRKAHGIETPVAFLTCNFSAPVGG
                     KPALFTHDEVITLFHEFGHGLHHLLTRVEEYGVSGIKGVEWDAVELPSQFMENFCWEW
                     EVLNHMTRHVDTGAHLPRVLFDKMVAAKNFQAGMQTVRQIEFSLFDMRLHCEFDPSGN
                     KTPLQLLEEVRDEVAVVRPPQWNRFPNNFSHIFAGGYAAGYYSYKWAEVLSADAYSLF
                     EENGVLSEEAGHRFWSEILAQGGTRPALESFVAFRGRQPTIDALLRHNGMTETADAA"
     gene            7185..>7855
                     /locus_tag="PKW44_05060"
     CDS             7185..>7855
                     /locus_tag="PKW44_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013031049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HQR50988.1"
                     /translation="MKLATWNVNSLKVRLPHVLEWLAANQPDVLCLQETKLEDANFPI
                     AEIEAVGYRPIFSGQKTYNGVALLGRTGGMDVVGAIPGFEDEQKRVLGATYGDTRVIC
                     AYFPNGQSLDSDKYQYKLHWLEAITAWLKKEVKTYPKLALLGDYNIAPEDRDVHDPKA
                     WEGNVLVSPAERAAFAALEALGLRDAYRLFDQPEAGFSWWDYRMAAFRRNLGLRIDHI
                     LLTSA"
BASE COUNT         1722 a         2251 c         2302 g         1580 t
ORIGIN      
        1 ccctggccgg taccgaagtg tccgcgctgc tgggccgtat gccttccgcc gtgggttacc
       61 agccgacgct ggccgacgaa atgggcaagc tgcaggaacg cattacttct accaaggttg
      121 gttccattac ctccattcag gccgtatatg tgccggcgga tgacttgacc gacccgtctc
      181 cggctaccac cttccagcat ttggattcga ccgtggtgct gtcgcgtgac atcgccgcgc
      241 tgggtattta ccctgctgtg gatccgcttg actcgacctc gcgccagctc gatccgttgg
      301 tggtgggtga agagcattac aacgttgccc gtagcgtgca gcaaaccctg caacgctaca
      361 aggagctgcg cgacattatc gccattctgg gcatggacga actgtcgccg gaagacaagc
      421 tggccgtggc gcgagcacgc aagatccagc gtttcctttc gcagccgttc cacgtggcgg
      481 aagtgtttac cggttcgcca ggcaagtacg tgacgctgaa ggacaccatt gcaggcttca
      541 aggcaatcgt caacggtgat tacgaccacc tgccggagca ggcgttctac atggtcggca
      601 gcattgaaga ggccgtggaa aaagccaaga cgctgtaata acttgaaggc gaaaaaccat
      661 tatggcaatg accatccatt tagacgtggt aagtgttgaa gagtcgatct tctcgggctt
      721 ggcggagttt gtcattgccc cggcggtgat gggcgaagtc ggcatttacc cgtttcacgc
      781 accgctgatc accaggatca agccaggtgc agtacgggtg aagcaaccgg acgtcgagga
      841 ggaaacggtg atttacgttt ccggcggcct gctggaagtt cagcccggcg ttatcacggt
      901 gctggcggat accgccattc gtgggcacga tctggatgag gcgatggcgc tggactccaa
      961 gcgcaaggcg gaggaagcca tgcgcgtcaa gggcgcccag atcgattacg ccaaggctca
     1021 ggccgagctg atcgaagcca cggcgcaatt gcgcgccatt caaaaactgc gcaagggcgg
     1081 cggacagcac taactttgtg ccatcgaccc gtttaaccaa ggcggctttt gggccgcctt
     1141 ttgttttata cttccgccca tgcaaaccag caaactcaat gttgtgatcc tcgccgcagg
     1201 caagggaacg cgcatggttt ccagcctgcc caaggtgctg caccaggtgg ctggaagctc
     1261 cctcctctcg cacgtcatcg cttgtgccga acagttgctg gcggacaata tcatggtcgt
     1321 ctatggctat ggtggcgaaa ccgtgcgcca ggcgattccg caggacaaca ttacctgggt
     1381 cagccaggct gaacaacttg gtaccgggca tgccgtgcag caggccatgc cgcacctgtc
     1441 ggcagatggc gtgtgtctga ttctgctggg tgacgtgcca ctcatcacgg cggacacctg
     1501 tcgcagcttg ctaaggaagg cggaacaggg gttggcgctg ctaaccgttg aaaagccgga
     1561 tccgaccggt tatggccgca tcctgcgtgg atttgatggc catgtccagg ccatcgtgga
     1621 agaaaaggac gccacaccgg aacagcgcgc ggtgcgcgag gtgaacagcg gcatcatggc
     1681 catgccaatc cagttgctca aggactggct gggccggctg gataaccgca atgcccaagg
     1741 tgaattttat ttgaccgata tcgtcggcat ggcggtacaa cagggtgtaa aggtgcagag
     1801 cgaactggca gaagacgaat gggaagttgc cgggatcaat tccaagcttg acttggcggc
     1861 agtagagcga gtttaccagc gcagccacgc cagacgcttg atgcaagcag gggtgacgct
     1921 gcgcgacccg gaacggctgg atgtgcgtgg tgagctgact accgggcacg atgtggaaat
     1981 tgacgttggc tgcgtgttcg aaggcaaggt gcaactcggc gacaacgtca gtataggccc
     2041 ctactgcgtg atcaaggatg ccgtactggc cacgggcagc gtcattgccg cctacagcca
     2101 catcgacagc gcccaggtcg gcgaaaacag tcgcatcggc ccttatgccc gcctgcggcc
     2161 tggaacagaa ctgatggcgg atacccatat cggcaacttc gtcgaaatca agaacagtca
     2221 agtcggccaa ggtagcaaga tcaaccacct gagttatgtt ggcgacagca cggtgggcaa
     2281 acaggtcaat atcggtgctg gcaccatcac ctgcaattat gacggggtca acaaataccg
     2341 taccgttatc ggagactgcg ccttcattgg ttcggatacc cagttggtgg cgccggtcac
     2401 tgtcgaagct ggtgccacca ttggcgctgg ctctacgatc acccgtgacg ccccggcaga
     2461 taccctcacc ctgtcgcgct ccaaacagat cacggtcgcg ggctggcgac gaccgcaaaa
     2521 gaaagcaaag taaaccaaat gtgtggaatc gttggcgcag ttgcccaaag aaatgtcgtc
     2581 cccatcctga tcgaaggcct gagccggctg gagtatcgtg gctatgactc ggccggcatc
     2641 gcggtgctgg acggcggtga aatcaggcgc gtgcgtgcga tcggccgcgt ggccgggatg
     2701 caacagaaag cacaggcaga gaaacttgat ggccggctcg gcatcggcca tacgcgctgg
     2761 gccacccatg gcggcgttac agaagccaat gcgcatccgc atgtgtcctg cggcgaaatc
     2821 gcggtggtgc acaacggcat catcgaaaac cacgacgagc agcgtaaccg cctgagagcc
     2881 ttgggctatg tatttgactc gcagaccgac acggaagtca tcgcgcatct gatccattat
     2941 tacaccaggc agggcaataa cctgcttgat gctacccagc atgcggtgcg ggaactggtc
     3001 ggtgcttatg cgattggcgt gatatcgctg ttggaacccc accacctggt atgcgcgcgc
     3061 atgggctgtc cgctgttgat cggactgggc gaaagcgaga atttcatcgc ctccgatgtt
     3121 tccgccgtgc tatcggtgac gcgccgcgtg atttacctgg aagatggcga tgttgccgac
     3181 ctaagcagcg atgccgtgaa cgtgttcgat caaaccggta acccggtgca gcgccgcgtg
     3241 cgtgtcagcg acgtatcgct cgcctcaatg gaactgggtc cctacgccca cttcatgcaa
     3301 aaggaaatcc acgaacaacc caaagccata tcagacacca tcgacaagat cctcgaagat
     3361 cgtttctctc caacgctgtt cggtgatgat gccgaacaaa gactgggcag cattgagggc
     3421 gtaaagattc ttgcctgtgg caccagctac tatgcaggct tggtggcagc ctactggctt
     3481 gaagccattg cagggataca gaccagcgtt gaaattgcca gcgagtatcg ctacagaaag
     3541 gccatagcca accctagaca ccttatcgtg acgctctcgc agtcaggcga aacgctggac
     3601 accatggaag ccctgaaata cgcgattgag cttggtcaac atcagacgct atccatttgc
     3661 aacgtgcaag aaagcgcgat accacgcgct agcagcatgg tgttttacac ccgagccggt
     3721 gccgagatcg gcgtggcatc caccaaagcc ttcacgacac aactggtggc tttgttcacc
     3781 ctttgcatca cgctcgcgaa gctgcgtggt cggatcactc cagaacagga agaacaattt
     3841 ctggaagacc tgcggcatct gccaggcaac gtacagtacg cgctgaatct ggaaccgcaa
     3901 atccgtgagt gggccaaggt tttcgccggc aagcaccatg cgctgttcct cggtcgcggc
     3961 gtgcactacc cgatcgcctt ggaaggcgcc ctcaaactca aggaaatttc ctacatccat
     4021 gcagaggcct accctgcagg cgagctaaag cacggccctc tggcgctggt ggataatgaa
     4081 atgccggtgg tggtcattgc gcccaacgat gccctgctgg aaaaagtcaa atccaacatg
     4141 caggaagtca gcgcccgtgg cggcgaactg ttcgtttttg ccgaccttta tagcaatttc
     4201 ggcgagcagc agggcatcca cgtcatccgc gcaccgcgcc acgttggtgt gctgtcacct
     4261 gtggtacacg ccatcccggt gcaactgctg gcctaccata ccgccctgct caaggggacg
     4321 gacgttgaca agccgagaaa cctggccaag tcagtcacgg tggagtagca acaaaaggca
     4381 ggaaatcgca gttattcagg acagtcggac tggcagattt ctcatggaac atagccgggt
     4441 gcattcggcc ccagattttg accgccatcc tgatagtcgt ccagatagcg atcattcgga
     4501 acgtagcctt cttcgctctc cccggtgatt tgtccattgg cgccaacatc aacctccttt
     4561 tcgccaacga ctacgccgtc ctgaacatta acttcttcca ttacatatgg gccactgcag
     4621 ctactgatga gcaagctggc cagaaaaaac atcagggtgc gtggttgcat gtgtgcgttc
     4681 cagaatatcg aaaattggtt ggatattaac ggattttagc gatttttgcg ccgaattccg
     4741 ctttattccc atgtaaacta cgttttattg agtatgactt actcttcagg agatttccat
     4801 gacggacacc agcgcggatg acaagaacat tgctgtattg acccacctgg ggggtatttt
     4861 tttcagcatt ttccccggcc tgatcgtctg gctgctgaaa aaggacgaca atgcctttat
     4921 cggcgaacag gcacgcgaag cgctcaactt ccagatcacg gtgctgatcg gctacctgat
     4981 agccgggctg ctgattctgg tgctggttgg catcctgctg tggccggtga tttatgtcgc
     5041 caatatcatt ctgtgcatca tcgcagcggt cagggccagc aagggtgagt attaccgcta
     5101 cccgttgacg ctgcggctca tcaattaagg attttaagtt tcatgcaaaa cccattgctg
     5161 catttctccg gcctgccgca tttcgaccag gtcaaacccg aacacgtcac ccccgccatc
     5221 gagcagctgc tgaccgaagg acgcgccttg gtggaacagc tggtcgccgc cagcgaagcg
     5281 ccgagctggg acaacttcgc ccgcccgctg gaggacatgg aggagcgtat ctctcgtgcc
     5341 tggtcgcagg tgtcgcacat gaattccgtg gtcaacagcc cggagctgcg cgaggcttac
     5401 aacgccaatc tgcccaagct gaccgcgttt tatgccgatc tttctcagca cgatgggctg
     5461 tacgcccggt tccgtgccct gcgtgccgat gcagggttcg agcagctcag cccggcgcag
     5521 cgcaagatcg tggaaaacga gctgcgcgat ttccgtctgg gcggcgccga attgccggaa
     5581 gaccagaaag cgcgcttcaa ggcaatccag gaggagcttt ccaccttgtc tgccaagttc
     5641 gaggaaaacc tgctggatac caccaatgac ttttcactat tggtagagaa tgcggccgag
     5701 ctggcgggta tcccggccga tgctctgcag gccgcgcgcg aagcggccga ggctgacagc
     5761 aagccgggct ggaaattcac cctgcatttt ccttcctaca tgccggtgct gcaatacgct
     5821 gacaaccgag cgctgcgcga aaagctgtac cacgcttacg ccacgcgcgc ctcggaattc
     5881 ggcaagcagg aatgggataa taccgcgctg attacgcaaa tcctcaaact tcgcctggaa
     5941 gcagcgcagc ttctgggctt cgacgactac gcgcaactct cgctggccac caagatggcg
     6001 caagcctcgg ccgaggtgat ggattttctg cgtacgctag gcctgcgtgg caaaccattc
     6061 gcggaaaagg acatgctgga gttgcgcgct catgctgccg accaattggg gctggctcag
     6121 ctggaagcct gggacattgc ctatgcctcg gaaaagctgc gtgaagagaa atacgccttc
     6181 tccgaccagg aggtgaagca gtactttccg gaattgcaag tgttgcatgg cctgttcaag
     6241 gtcactgaaa ccatcttcgg cgtgcaggtc aggaaaatcc aggcaccggt atggcaccag
     6301 gatgtcagct tttacgaaat tactgaccgc aacggccgct tggtcggcca gttctatctc
     6361 gacctctatg cccgcaacgg caagcgtggc ggggcctgga tggacgaggc catcacccgc
     6421 cgccgcaagg cgcatggcat cgaaacgccg gtggctttcc tcacttgcaa cttttccgcg
     6481 ccggtgggcg gcaagccggc gctgttcaca catgacgaag tcatcaccct attccatgaa
     6541 ttcggccacg gcttgcatca cttgctgacc cgggtcgagg aatacggcgt gtccggcatc
     6601 aagggcgtgg aatgggacgc ggtggaactg cccagccagt tcatggaaaa cttctgctgg
     6661 gaatgggagg tgctgaacca catgaccagg catgtagata ccggcgcgca cctgccccga
     6721 gtgctgttcg acaagatggt cgccgccaag aacttccagg ctggcatgca gaccgtgcgc
     6781 cagatcgagt tctcgctgtt cgacatgagg ctgcattgcg aattcgaccc gagtgggaac
     6841 aagacgccat tgcaacttct tgaggaagtg cgcgacgagg tcgccgtggt gcgtccgcca
     6901 cagtggaacc gcttccccaa taacttctcg cacatcttcg ccggtggcta tgcggccggt
     6961 tattacagtt acaagtgggc ggaagtgctg tcggccgacg cctacagcct gttcgaggaa
     7021 aacggcgtgc tgtcggagga agccggccac cgcttctgga gcgaaatcct ggcgcagggc
     7081 ggcacacggc cggcgctgga atctttcgtc gcctttcgtg gtcggcagcc gaccatagat
     7141 gccctgttac gccacaacgg catgaccgaa accgcagacg cggcatgaag ctcgccacct
     7201 ggaacgtcaa ttcgctgaaa gtgcggctgc cgcacgtgct cgaatggctc gccgccaatc
     7261 agcctgacgt attgtgcctg caggaaacca agctggaaga tgccaatttt cccatcgccg
     7321 aaattgaggc ggtaggctac cggccgattt tcagcgggca gaaaacctac aacggtgtgg
     7381 cgcttctggg cagaaccggg gggatggatg tcgtcggtgc gatccccgga ttcgaggacg
     7441 agcagaagcg cgtgctgggc gccacctatg gtgatacgcg cgtgatctgc gcctacttcc
     7501 ccaatggcca aagcttggac tcggacaagt accagtacaa gttgcactgg ctggaagcca
     7561 ttactgcgtg gctcaagaag gaagtaaaga cttacccgaa gctcgccctg ctgggggact
     7621 acaacatcgc gccggaagac cgcgacgtgc atgaccccaa ggcctgggaa ggcaacgtac
     7681 tggtcagccc ggccgaacgt gccgcgttcg ctgcgctgga agctttaggg ttgcgagatg
     7741 cctaccgtct gttcgatcag cctgaagctg gcttcagctg gtgggattac cgcatggccg
     7801 ctttccgccg caaccttggg ctgcgcatcg accacattct gctaacttct gcctt
//
LOCUS       DAOMFA010000074         7826 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_1100_length_7826_cov_5.097156, whole genome shotgun sequence.
ACCESSION   DAOMFA010000074 DAOMFA010000000
VERSION     DAOMFA010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 7826)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7826)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7826
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1100_length_7826_cov_5.097156"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            56..808
                     /locus_tag="PKW44_05065"
     CDS             56..808
                     /locus_tag="PKW44_05065"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acyltransferase"
                     /protein_id="HQR50989.1"
                     /translation="MLNQRVVNVLQQEPSALYSSMARTSADVMEAQRLRYKIFAEEMG
                     AKLSGHDGLDRDGFDPFCDHLLVREREYGKVVGTYRILSPAMANEVGGYYSSGEFDLN
                     RIEHLFDHTVEVGRACVHRNYRSGSAIALLWAALARYMQTNRYDTLIGCASISMADGG
                     HLAASLYNRLKHEYLSPPEYRVFPRCPLPLDALRSDIEDAPCPALVKGYLRLGAWVCG
                     EPAWDPDFNTADLLVMLPMSRINRRYAAHFLK"
     gene            811..1617
                     /locus_tag="PKW44_05070"
     CDS             811..1617
                     /locus_tag="PKW44_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HQR50990.1"
                     /translation="MQGAATSFATRLFRLIRLALHIFHGVLTAATVLDRMEQGRRQEV
                     ILRWSRQLLDILKVRTTLHGQLPSPGQRGTLFVANHVSWLDIYALKATHPMHFVAKSE
                     IRGWPVVGWLAHITDTIFIERDRRHATGQAVTALEQALRQGECLCFFPEGTTTDGTQL
                     KPFKASLLQAAINAEAALWPLAIRYLNADGSANTAVAYHGEMTMLQSLWEVLKQRETR
                     VELHFAAPISATGGERRHLSQQARHAISSLLHLPRHKAPGTHDGPPGASR"
     gene            complement(1532..2365)
                     /locus_tag="PKW44_05075"
     CDS             complement(1532..2365)
                     /locus_tag="PKW44_05075"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="symmetrical bis(5'-nucleosyl)-tetraphosphatase"
                     /protein_id="HQR50991.1"
                     /translation="MATYAVGDIQGCYTSFTHLLQHIGFSPVRDRLWLVGDIINRGPD
                     SLAVLRWAMQHQDSLEVVLGNHDLHALALAEGFVEAHRYDTVQALFEAPDREELLTWL
                     RGRRMAHAEGEYLMVHAGVLPQWDASLTLTLAAEVETVLRSDDYRYFLSHMYGNHPNR
                     WDGELRGMSRLRVITNALTRLRVCTLTGDMDFRFKGELSNIPAGYLPWFELENRNSSG
                     STLLFGHWSALGLLVRDNLIGLDTGCLWGGQLSAMRLEDRRVFQVPCAAEDAIKKIWR
                     A"
     gene            complement(2413..2943)
                     /locus_tag="PKW44_05080"
     CDS             complement(2413..2943)
                     /locus_tag="PKW44_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HQR50992.1"
                     /translation="MSTQHHDFPKTTVLQFILALLGGLLMPGLVIFLIVKLVLGIQAT
                     HIEDTDPAIAAARVVERIQPVGQVNLADSSGPRVDKSGEQVVKEVCAMCHAAGLLGSP
                     KIGDKSAWAPRLSQGYDTLVKHALEGIRQMPARGGAADLSDGEVAAAVAYMANQSGAS
                     FKTPEPARAEAEVARK"
     gene            3052..3128
                     /locus_tag="PKW44_05085"
     tRNA            3052..3128
                     /locus_tag="PKW44_05085"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3086..3088,aa:Arg,seq:ccg)
     gene            complement(3269..3907)
                     /locus_tag="PKW44_05090"
     CDS             complement(3269..3907)
                     /locus_tag="PKW44_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018229714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HQR50993.1"
                     /translation="MQQILVYADSLSWGIIPLTRQRLPFDRRWPGVLEMALCASGRNV
                     RVIEDCLNGRRTVWDDPYKPGRNGLLGLAQRIEIHSPLTLVILMLGTNDFQSMHEHNA
                     WHASQGISALVSAIRAAPIEPGMPIPNVLVVSPPAIQTPQGPIAPKFAGAERKCVGLA
                     DAYQKVSKELKCYFFDAGCITTTSTIECVHLDAEQHMALGNALSKIVMPLLS"
     gene            4203..6329
                     /locus_tag="PKW44_05095"
     CDS             4203..6329
                     /locus_tag="PKW44_05095"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013291999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HQR50994.1"
                     /translation="MKNRFLWLLLAIALSAQAATPNTATPSAQLKPLPEHAQAAHMAA
                     EVLTRYHYRHIPLDDKSSSKIFDNYLKALDEEKLFFTQSDIDRFSIARTWLDDAIFNE
                     NLSAPFAIHNAFQQRASERIAYARSLLKKGFDFDAKESSQITREKAAWAKSEAELNDL
                     WRKRVKNDWLRLKLAGKDDKGIVETLDKRYENSLNSSLKIKSDDVFQNFMNAYATAID
                     PHTNYFGIRASEDFDISMRLSLVGIGAVLQDKEGYTTIRELMAGGPAALSGKLKVGDR
                     IVGVGQGENGTPTDVVGWRIDDTVARIRGAEDSVVVLDILPAEAGLDGKHKLIPLVRK
                     KISLDKQAAKKSVIEVKDAGATRRVGVISLPGFYQDFAARQKGDKDFKSATRDVARLL
                     EELKKDNVDGVLVDLRNNGGGSLDEAVELTGLFIDKGPVVQQRDSEGKISVEGDTNAG
                     QAWSGPLGVLINRASASASEIFAAAIQDYGRGIVIGEQSYGKGTVQSVVDLDQLAKNE
                     KPEYGELKMTIAQFFRINGGTTQLRGVTPDIGLPTMIDAEEFGESSYDNALPWTQIRA
                     VDYAPAGDLTAILPKLKATHESRISRDKDFSYLREDIAEADAQRKKNLISLNETERRK
                     EREAREARLKLQEKDSEADKGAITSRLQDDGLQSSERDLNDDLALEKARKDAKDIFLD
                     EAAHILSDEVGLLKASPRLSASVLPK"
     gene            complement(6396..7784)
                     /gene="cysG"
                     /locus_tag="PKW44_05100"
     CDS             complement(6396..7784)
                     /gene="cysG"
                     /locus_tag="PKW44_05100"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041353255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HQR50995.1"
                     /translation="MNQFPIFIQVRDRRCLVVGGGEIATRKISLLLKAEAQVAVVAPD
                     LCQSLAELYAAEKIFWEKALFDPRHLDQAWLVIAATDDKAINAAVSQAAQARSIPVNV
                     VDQPALCTFTMPSIIDRSPIVVAISSGGAAPVLARLLRARLETLIPAAYGRLAALAGK
                     FRKQVQQQLPEGSARRIFWENVFEGPIAEQMLAGREEAAFSALQQSLKQAVNHPPGGE
                     VYLVGAGPGDPDLLTFRALRLMQKADVVLYDNLVSEAVMDLVRRDAERIYVGKKSSIH
                     TLPQEEINMLLVRLAKEGKRVLRIKGGDPFIFGRGGEEIETLAEHGIPFQVVPGISAA
                     NGVSSYAGIPLTHRDYAQAVTFVTGHLKDGTVNLDWPALVRPNHTVVIYMGLEGLPEI
                     CKKLIEHGLPPQHPIAVVQQGTTRHQRVMTGTLSDLPGRVAAEGFKAPTLTIVGEVVG
                     LREKLAWFDPRG"
BASE COUNT         1662 a         2284 c         2274 g         1606 t
ORIGIN      
        1 ctacggtaac gtacgctgct taaagtttct cccaacattc cagttgggag aaaccatgtt
       61 gaatcaacgc gttgtcaatg tgctgcagca ggagccgtcc gctctttatt caagcatggc
      121 ccgcacttcg gccgacgtca tggaggcgca acgtttgcgc tacaagatat tcgccgaaga
      181 gatgggtgcc aagctgtccg gccatgacgg gctcgaccgg gacggtttcg acccgttttg
      241 cgatcatctg ctggtgcgcg agcgggaata cggcaaagtc gtcggcacct atcgcatcct
      301 cagccccgcc atggccaacg aggttggggg ctattattcc tccggcgaat tcgatctgaa
      361 ccggatcgaa catctgttcg accacacagt agaggtcggc cgcgcctgcg tgcatcgcaa
      421 ttaccggagt ggcagcgcca ttgccctgct ctgggccgca ttggccagat acatgcagac
      481 caaccgttat gacacgctaa tcggctgcgc cagcattagc atggccgacg gtggacacct
      541 tgctgccagt ctctataata ggctgaaaca tgaatacctt tccccgcccg aataccgagt
      601 tttcccgcgt tgcccgctac cgctggatgc cttgcgcagc gacatcgagg atgcgccctg
      661 tccggcgctg gtcaaaggct atctgcgcct cggcgcctgg gtttgcggcg aaccggcctg
      721 ggatcccgac ttcaataccg cggacctgct ggtgatgttg ccgatgtcgc gcatcaaccg
      781 ccgctacgcc gcgcatttcc tcaaatagcg ttgcaaggcg cggccacttc ctttgccacg
      841 cgcctgttcc gcctgatacg gctggcgctg catatttttc acggcgtcct gaccgccgcc
      901 accgtgctgg accgtatgga gcaggggcgc cgccaagagg tgatcctacg ctggtcgcgg
      961 cagttgcttg atattctcaa ggttcgtacc acgctgcacg gacaacttcc gagtccgggg
     1021 cagcgcggca cactgttcgt cgccaaccac gtttcctggc tcgacatcta tgcgctcaag
     1081 gccacgcacc ccatgcattt tgtcgccaag tcggaaatcc gcggttggcc agtggtcggc
     1141 tggctggcgc acatcaccga caccattttc atcgaacgtg atcgccgtca tgcgactggg
     1201 caagcggtga cggcgctgga gcaggcgctg cggcagggcg aatgcctgtg ttttttcccg
     1261 gagggcacca ccaccgatgg cacgcagctc aagccgttca aggccagctt gctacaggcc
     1321 gccatcaatg ccgaggcagc gctgtggccg ttggcgatcc gttacctgaa tgcggatggc
     1381 agcgccaaca ctgctgtcgc ctatcacggc gagatgacta tgctgcaatc gttatgggaa
     1441 gtcttgaaac agcgtgaaac gcgggtggaa ctgcactttg ccgcgccgat cagcgccacg
     1501 gggggcgaac gccgtcacct gtcacagcag gctaggcacg ccatatcttc tttattgcat
     1561 cttccgcggc acaaggcacc tggaacacac gacggtcctc caggcgcatc gcgctgagtt
     1621 ggccgcccca gaggcagccg gtatccagcc cgatcaggtt gtcgcgcacc agcaaaccca
     1681 gcgccgacca gtgcccgaac aacagcgtgc tgccgctgct gttgcggttc tcaagctcga
     1741 accaaggcag gtagccggcc gggatgttgg atagctcgcc cttgaaacgg aaatccatgt
     1801 cgcccgtcaa tgtgcaaacg cgcaaccggg tgagggcgtt ggtaatcacg cgcaggcggc
     1861 tcatgccgcg cagttcgcca tcccagcggt tagggtggtt gccatacatg tgcgagagaa
     1921 aataacgata gtcgtcgctc ctgagcacag tctcgacttc ggccgctagg gtaagggtaa
     1981 ggctggcgtc ccattgcggc agcaccccgg catgtaccat caggtattcg ccctcggcat
     2041 gggccatacg cctgccgcgt agccatgtca gcagttcttc gcggtccggt gcctcgaata
     2101 gcgcttgcac ggtatcgtaa cggtgggcct cgacgaaacc ttcggccagc gccagggcgt
     2161 gcaggtcgtg gttgcccagt accacctcca gcgaatcctg gtgctgcatc gcccagcgca
     2221 agacggccag cgaatccggc ccgcggttga tgatgtcgcc caccagccac aaacggtcgc
     2281 gtacagggct gaagccgata tgttgcagca ggtgggtaaa ggaggtgtag cagccctgaa
     2341 tgtcgccgac cgcgtaggtt gccattgtat tggactctca gggaatcagg ctggcggcag
     2401 gctcggcaaa aactatttcc tggcgacttc ggcttcggcc cttgccggct ccggagtctt
     2461 gaagctggcg ccagactggt tggccatgta ggccaccgcg gcggccactt cgccatcgct
     2521 aagatccgca gcgccgccgc gcgccggcat ctggcggatg ccctccagcg catgcttcac
     2581 cagtgtgtcg tagccttgcg aaaggcgtgg cgcccaagca ctcttgtcgc caattttcgg
     2641 cgaacccagc aagccggccg catggcacat ggcgcagact tccttgacga cctgttcgcc
     2701 actcttgtct acgcgcggac cgctgctgtc ggccagattc acctgcccca ccggctggat
     2761 gcgttctact accctggccg cggcgatcgc cgggtcggta tcctcaatat gggtggcctg
     2821 aatgcccagc accagtttga cgatcaggaa gatgaccagc cccggcatca gcaatccgcc
     2881 cagtaacgcc aggatgaact gtagaaccgt ggttttcggg aagtcgtgat gctgcgtgct
     2941 catggcaaaa gtcctggtcg agatttataa aacacaaatt ataccggcaa gcacctcgcg
     3001 gacaaaggac agcgaggaca ttgacggggg tgtttgctat aattcgcccg tgcgcccgta
     3061 gctcagttgg atagagtatt ggtttccgaa gccaagggtc actggttcga atccagtcgg
     3121 gcgcgccaca gtcaacgaaa ggccgattcc tctacgaaat cggccctttt gcatttcaat
     3181 cgcaggcgat gaatcttggc actcagcctg ctgtaaattc attaagtctg aatttggccc
     3241 atttagccat tcaacttttc tttttacttt atgaaagtag aggcattact atcttagata
     3301 gagcatttcc aagtgccatg tgctgctcgg catcaagatg cacacactct atagtactgg
     3361 tggttgttat gcagccagca tcaaaaaaat aacattttag ttctttactg accttctggt
     3421 atgcatctgc caatccaaca catttccttt cagcgcctgc aaatttagga gcaatcggtc
     3481 cttggggagt ctgaatggcc ggtggtgaaa caaccaagac gttgggtata ggcattcccg
     3541 gttctatcgg cgctgcacga attgcggata caagcgcaga aatcccttga gaagcgtgcc
     3601 acgcgttgtg ctcatgcatg gattgaaaat cgttggttcc cagcataagt attacaagcg
     3661 taagtggcga atgaatttcg atgcgctggg caagtcccaa taggccattc cgtccgggct
     3721 tgtagggatc atcccatacg gttctgcggc cattaagaca atcttctatg acgcgaacgt
     3781 tcctgcctga agcacaaagt gccatctcta atacaccggg ccagcgccga tcaaatggca
     3841 agcgttggcg ggtaagcgga atgatgcccc aggacagtga atccgcataa acaagaattt
     3901 gctgcataca gcaccatgcc ttgaagattt gatgtttgtt tatgctgcca tttttttaga
     3961 atgtccgcta ttcgccaata gtcgtcatta ccaaatatag agctacctcc tgtttccagt
     4021 gtccgggttt atcgactcgg gcgcgccatc aagctcatat tcccagcgat ggtgcttgtc
     4081 gcctgatcga gaacttatac ggtaatttag ctgtctcttc tgcgaggcgg cgggtctgat
     4141 acactcacgg gaacatctcc tgccgattct gctgattgat gaccaacttc cgggactgac
     4201 tgatgaaaaa cagattcttg tggctattgc ttgcgattgc cctgagcgcg caagcggcaa
     4261 caccgaatac agcaacgcca tccgcgcaac tcaagccatt gccggaacat gcgcaggccg
     4321 cacacatggc ggctgaagtg ctgacccggt atcactacag gcacattcct ctggatgaca
     4381 agagctcttc gaaaatattc gacaattatc tgaaggcgct ggatgaggaa aaattatttt
     4441 ttacgcaatc ggacatagat cggttctcga ttgcgcgaac ttggcttgac gacgccattt
     4501 tcaacgagaa cctgagcgcc cccttcgcca tacacaacgc ctttcagcag cgcgcctccg
     4561 aacgcatcgc ctatgcgcgc tccctgctca agaaaggatt tgatttcgac gccaaggaga
     4621 gctctcagat tacccgcgag aaggctgcgt gggcgaagtc cgaggccgaa ctgaacgact
     4681 tgtggcgcaa gcgcgtcaag aacgactggc tgcgtctaaa actggccggc aaggatgaca
     4741 aaggcatcgt cgagaccctg gataaacgat atgagaattc cttgaacagc agcctcaaga
     4801 tcaagagcga cgacgtcttt cagaacttca tgaacgccta cgccacggca atcgacccgc
     4861 acaccaatta tttcgggatc cgtgcttcgg aagatttcga tatttccatg cgcttgtcgc
     4921 tggtcggcat aggcgcagtg cttcaggaca aagaggggta caccacgatc agggagctga
     4981 tggcaggcgg gccggcagcg ctttccggca agttgaaggt gggtgaccgg atcgtgggtg
     5041 tcgggcaggg cgaaaacggc acgccgaccg acgtggtggg ttggcgcatc gacgacacgg
     5101 tagccaggat tcgcggtgcc gaggattcgg tggtcgtcct cgatatcctg cccgcggagg
     5161 ccggcctgga cggcaagcac aagctaattc cgcttgtccg caagaaaatc agcctggata
     5221 aacaagccgc gaagaaatct gtcatcgagg tgaaagacgc tggtgcgaca cgtcgtgtcg
     5281 gcgtgatctc cctgcccggg ttctatcagg atttcgcggc tcgccaaaaa ggcgacaagg
     5341 atttcaaaag cgcgacccgc gacgtggcgc gcttgctgga ggagctcaaa aaagacaacg
     5401 tggatggcgt gctggtcgat ctgcgcaaca atggcggcgg ttcgctggat gaggctgtcg
     5461 agctgacggg gctgttcata gacaagggtc cggtcgtgca gcaacgtgac tccgaaggca
     5521 agatttcggt ggaaggcgac actaacgccg gccaagcctg gagcggaccg ctgggtgtgc
     5581 tcatcaaccg cgcctcggcg tcggcctccg aaattttcgc ggcggcgata caggattacg
     5641 ggcggggtat cgtgatcggc gagcaaagct acggaaaagg cacggtacag tccgtggtag
     5701 acctggatca gctcgcaaaa aacgaaaagc cggaatacgg tgaactcaag atgacgatcg
     5761 cccaattctt ccgcatcaac ggcggcacca cccagttgcg tggcgtcacg ccggatatcg
     5821 gtctgccgac gatgatcgac gccgaggagt tcggcgagtc cagctatgac aatgccttgc
     5881 cctggacgca gattcgggcg gttgactatg cccccgccgg cgacttgacg gccattctgc
     5941 ccaaactgaa ggcgacccac gagtcgcgga taagccggga caaagacttc agttatttgc
     6001 gggaagacat cgccgaggcc gatgcccagc gcaagaaaaa cctgatttca ctgaacgaaa
     6061 ccgagcggcg caaggagcgt gaagcgcgag aggccaggct gaagctgcag gaaaaggaca
     6121 gcgaagccga caaaggtgcc ataacttcca ggctccagga tgatggcctg cagtccagcg
     6181 aacgcgacct caacgacgat ctggcgctcg aaaaagcaag gaaagatgcc aaagacatct
     6241 ttctggacga ggccgcgcat atcctcagcg atgaagttgg tttgctgaag gccagtccca
     6301 gactttcggc cagcgtgcta cccaaatagg cgcaacccgc tttcggcccc aagtcggggg
     6361 ggctgttgtc gtggctgggc ggctctatcg cgagatcacc cccgaggatc gaaccacgcc
     6421 agtttttccc ggagccctac cacttcgcca acgatagtca gcgtcggcgc cttgaatccc
     6481 tccgctgcca cccgcccggg cagatcggac agcgtgccgg tcatcacgcg ctggtggcgt
     6541 gtggtgcctt gttgaacaac ggcaatgggg tgttgcggcg gcaggccgtg ttcgatcagc
     6601 ttcttgcaaa tttcaggcag cccttccagc cccatgtaga tcactaccgt gtggttcggc
     6661 cgcaccagcg caggccagtc gaggttgacc gtgccgtcct tgagatgacc ggtaacgaaa
     6721 gtcacggcct gggcataatc acggtgagta agcgggatgc cggcatagct ggaaaccccg
     6781 ttggccgcgg agatccccgg caccacctgg aacgggatgc cgtgttcggc cagcgtttcg
     6841 atttcctcgc cgccccgtcc gaagatgaac gggtcgccgc ccttgatgcg cagtacccgt
     6901 ttgccttcct ttgccagtcg taccagcagc atattgattt cctcctgcgg cagggtgtgg
     6961 atgctgcttt tcttgccgac atagatgcgc tctgcatcgc gtcgtaccaa gtccatcacg
     7021 gcctcggaga cgaggttgtc gtagagcacc acatcggctt tctgcatcag ccttaacgca
     7081 cgaaaagtca gcaggtcggg gtctccaggg cctgccccga ccagatagac ctcccctccc
     7141 ggaggatggt tgacggcctg tttgagcgac tgctgcaagg cgctgaatgc cgcctcttca
     7201 cgcccggcca gcatttgctc ggcgatcggg ccttcgaaca cgttttccca gaaaatgcgc
     7261 cgggccgaac cttccggcag ttgctgctgg acctgcttgc ggaatttccc ggctagcgcg
     7321 gccagccgtc cgtaagccgc cgggatcagc gtttccagcc gcgcgcgcaa caggcgcgca
     7381 agcacgggtg ccgcgccacc gctggaaatg gcgacgacga tgggcgagcg gtcgatgatg
     7441 gaaggcatgg taaaggtgca gagcgcgggc tggtccacca cattgaccgg gatgctgcgc
     7501 gcctgcgcgg cctgcgagac ggctgcgttg atggccttgt catccgtggc ggcaataacc
     7561 agccatgcct gatcaagatg ccgggggtcg aacagcgctt tttcccagaa tattttttcc
     7621 gcggcataca gttcggcgag gctttggcag agatcaggcg ccactacggc aacttgtgct
     7681 tctgctttca gcagcagcga aatcttgcgc gtggcgatct ctccgccgcc gacgaccagg
     7741 cagcgccggt cacggacttg aatgaatatg gggaactgat tcatgtgtgt aatttatcct
     7801 gaattcattc ttgtgaaact tgattc
//
LOCUS       DAOMFA010000075         7463 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_1197_length_7463_cov_5.093683, whole genome shotgun sequence.
ACCESSION   DAOMFA010000075 DAOMFA010000000
VERSION     DAOMFA010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 7463)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7463)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7463
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1197_length_7463_cov_5.093683"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(240..824)
                     /locus_tag="PKW44_05105"
     CDS             complement(240..824)
                     /locus_tag="PKW44_05105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR50996.1"
                     /translation="MRKPALPLPILILLPLLLPGCLGAPVARQLASSAFTQGADHITG
                     KIVENQEEKQRQQSRDLTFKDSVGDEYWAIFAVSQFQDAPPTPPAAEAATPAPPSATA
                     SRLVPVEIWGVVVGQEKTSVLERMRRNGSQIVPSQEEWDQWQLATGGLEGKSGTQLYL
                     LLPPDFGRVRSGDRAIAEIAGVGGVHIARYRAEN"
     gene            complement(882..1232)
                     /locus_tag="PKW44_05110"
     CDS             complement(882..1232)
                     /locus_tag="PKW44_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS/SEC14 domain-containing protein"
                     /protein_id="HQR50997.1"
                     /translation="MITINNESQNIIVAAALGEFTLADFQELEKATEHVFKFQGKARL
                     LVDLRDMIDFTVDVAWEEIKLMRGHPHDFERIAIVTDNQWLQWSGWIGRIFTDAEVLV
                     FDDYDTALAWVGEH"
     gene            complement(1252..2154)
                     /locus_tag="PKW44_05115"
     CDS             complement(1252..2154)
                     /locus_tag="PKW44_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HQR50998.1"
                     /translation="MKPTTYGSLWMLAAGVFFACMGALVKIGAQKFTSAELVFYRSLF
                     GLLAILLLSLAGRKRLATRHWKPHLSRSLFGFAALVLFFYAIGKLPLATAVTLNYTSP
                     LFLAALSLIYLRGEARPALLLAIVIGFGGVALLLQPSFSGDDWLAALLGLASGLLAGV
                     AYLQVTQLGRMGEPEWRTVFYFTLVCTIGSGLWMLIHQFHALTLPDLLLLLAIGACAT
                     LAQLAMTRAYRKGDPLVAGSLAYSTVVLASLLGILLWGETLSLASWLGVALITGSGVI
                     SVRSQPLPRGDAGDFDKTIQAPTL"
     gene            complement(2174..3280)
                     /locus_tag="PKW44_05120"
     CDS             complement(2174..3280)
                     /locus_tag="PKW44_05120"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HQR50999.1"
                     /translation="MSLAQITPIKRQPRAQLPVSWYVDPAIYELERQALFPHAPAYVG
                     HALTVPNPGDYHALEWMNHAKALVRNDSGIELISNVCRHRQALMLKGKGNAQNIVCPL
                     HRWTYDLQGELMGAPHFEQQPCLNLSKTPLQNWNGLLFDSRRDVARDLGKLGFLKDFD
                     FSGFMLDRVMVDEYQFNWKTFIEVYLEDYHVAPFHPGLGNFVDCDDLKWEFGEWYSVQ
                     SVGINHALQKSGSPAYQRWQEQVLRYGNGELPKYGAIWMVYYPFLMIEWYPHVLVVSN
                     IIPRGPNACTNVVEFYYPEDIALFEREFIEAEQAAYTETAIEDEDICQRMHDGRQALF
                     AQDLDEYGPYQHPMETGMEHFHLWMRAQLEPHLK"
     gene            3645..4136
                     /locus_tag="PKW44_05125"
     CDS             3645..4136
                     /locus_tag="PKW44_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate hydroxylase PuuD"
                     /protein_id="HQR51000.1"
                     /translation="MEFAPAIARWIHLLAGVAWVGLLFYFNLVQVPALKAAAADGTAA
                     GITRHVAPRALLWFRWAAVVTWLAGASLLGANFTAAFTLRPDFAPIGIGAWLGTIMLF
                     NVWVLIWPNQQKVLGMKPASDEEKNRARRVALLASRTNMMLSIPMLFFMAGGLTHRAA
                     FGF"
     gene            4206..5387
                     /locus_tag="PKW44_05130"
     CDS             4206..5387
                     /locus_tag="PKW44_05130"
                     /EC_number="2.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine transaminase"
                     /protein_id="HQR51001.1"
                     /translation="MSQHLMNTYGRQPVTFVKGEGVWLWDQQGKRYLDGLAGVAVNGL
                     GHAHPRLTAAICEQASRLIHVSNIYRIDEQAKLADRLAEISGMDRIFFCNSGCEANEA
                     AIKLARLYGHSKDIEHPEIVVMDKAFHGRTLATLSATGNRKVQAGFEPLVPGFVRVPF
                     DDMDAVRQVARHNPNVVAVLVEPVQGEGGINIPHDGAGYLRGLREICDANDWLLMLDE
                     VQSGIGRTGTWFAFQHAGIVPDVMTLAKGLGSGVPIGACLARGAAAEVFTPGKHGSTF
                     GGNPLACAAALATLDAIEQEKLRENAETIGNFICDGFRQAFAGVAGVVTIRNAGLMIG
                     IELDRPCGELVKQALEAGLLINVTADTVVRLLPPLVMNREEAQQLVDILAPLVKGFLG
                     R"
     gene            5406..6326
                     /gene="argF"
                     /locus_tag="PKW44_05135"
     CDS             5406..6326
                     /gene="argF"
                     /locus_tag="PKW44_05135"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HQR51002.1"
                     /translation="MAIKHYLQFKDLNRDELDYLFERTRWIKQQFKSYQQYWPLTDRT
                     LVMIFEKASTRTRLSFEAGIQQLGGSAIYLNTRDSQLGRGEPVEDAAQVISRMSDIVM
                     IRTFEQEIIERFASHSRVPVINGLTNEYHPCQILADIFTYIEHRGPIQGKTVAWIGDS
                     NNVCNTWLQAAEVLDFNVHVSTPPGYEVEAERAGLYGHDHYEEFADPMEAAQGADLVT
                     TDVWTSMGFEAENEERLRDFADWQVDADMMRVAAKDAVFMHCLPAHRGEEVAAEVIDG
                     PQSVVWDEAENRLHVQKALMEYLLLGKVSQ"
     gene            6323..>7463
                     /locus_tag="PKW44_05140"
     CDS             6323..>7463
                     /locus_tag="PKW44_05140"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HQR51003.1"
                     /translation="MSQINKVVLAYSGGLDTSVILKWLQDTYQCEVVTFTADIGQGEE
                     LEPARAKALKFGIKPEQIYIDDLREEFVRDFVFPMFRANTVYEGEYLLGTSIARPLIA
                     KRLIEIARETSADAISHGATGKGNDQVRFELGAYALNPNIKVIAPWREWDLLSREKLM
                     AYAETHGIPVDMKHKQGGSPYSMDANLLHISYEGRHLEDPAAEAEESMWRWTVSPEQA
                     PDAPEYLDIEYVNGDPVALNGKKLAPHALLAELNRLGGKHGIGRLDLVENRYVGMKSR
                     GCYETPGGTIMLKAHRAIESLTLDREVAHLKDDLMPRYASLIYNGYWWSPERVALQTL
                     IDHTQKSVNGFVRVKLYKGNVIVVGRDSKTDSLFDPTIATFEDDAG"
BASE COUNT         1440 a         2188 c         2376 g         1459 t
ORIGIN      
        1 ttgcgcgcgc cctcgctggt cttttgtttt gtatggaaat tttgttcagt cgggcgtatg
       61 aatcactacg ccgctccttc acaaaattcc cctacaaaac aaaatcctgc acgatcatca
      121 cgcgaattca tgaaacatgc gggtcagatg atagggtagg ggggtgcctc gccccgatca
      181 aggtcagctg ccagggctgt aatgcaatcg cgccgaggcg gcgctcctgc gctgcttgcc
      241 taattttcgg cccggtagcg ggcgatatgc acgccgccga ccccggcgat ttcggcaatg
      301 gcccggtcgc cgctgcggac gcggccgaaa tccggcggca gcagcaaata aagctgcgtg
      361 cctgacttgc cttcaagccc gccggttgcg agctgccact gatcccactc ttcctggctg
      421 gggacgatct gtgatccgtt gcggcgcatc cgttccagca cgctggtttt ttcctgtccg
      481 accacgactc cccatatttc caccggaacc aggcggctgg ccgttgcact tggcggggcg
      541 ggtgtggccg cttcggcggc gggaggcgtg ggcggcgcgt cctgaaactg tgatacggca
      601 aagatggccc agtattcgtc ccccacgcta tccttgaacg tcaggtcgcg cgattgttgg
      661 cgctgcttct cttcctgatt ctcgactatt tttccggtaa tgtggtccgc gccttgcgtg
      721 aaagccgaag aggcaagctg gcgcgcgacc ggcgcaccca gacatcccgg cagcagcagg
      781 ggcaggagga tcagtattgg cagcggtaat gccggtttgc gcatggatgt ccgcgattca
      841 ggtagccgtc agctaagttt gatggctgcg gacgaacacg gttagtgctc ccccacccac
      901 gccagggcgg tatcgtaatc atcgaacacc agcacctcgg cgtcggtgaa gatgcggccg
      961 atccagccgc tccattgcag ccactggttg tcggtgacga tggcgatacg ctcgaaatcg
     1021 tgcgggtgcc cgcgcatcag cttgatttcc tcccacgcca cgtctaccgt gaagtcgatc
     1081 atgtcgcgca ggtcgaccag caggcgcgcc ttgccctgga acttgaagac atgctcggtg
     1141 gctttttcca gttcctgaaa gtcggccagg gtgaattcgc ccaatgcggc ggcgacgatg
     1201 atgttttgcg actcgttatt gatggtaatc atgcgggttc ccttgagttg gctatagtgt
     1261 aggcgcttgt atggttttgt caaaatctcc ggcatcgcct cgcggcaatg gctggctgcg
     1321 tacgctgatg acgccgctgc ctgtgatcag cgccacgccc agccaactgg cgagagaaag
     1381 cgtctctccc cacagcagga tgccgagcag gctggccagc accacggtgc tgtaggccag
     1441 gctgcccgct accagcgggt cgcccttgcg gtaggcgcgc gtcatggcca gttgtgccag
     1501 ggtggcgcag gcgcctatgg ccaaaagcag cagcaggtcg ggcagggtga gggcgtgaaa
     1561 ctggtggatc agcatccaca ggccgctgcc gatggtgcag accagcgtga aataaaatac
     1621 ggtacgccat tccggctcgc ccatgcgccc cagttgggta acctgcaggt aggcgacgcc
     1681 ggccagcagg ccggaggcca ggccgagcag ggcggccagc cagtcatcgc cggaaaagct
     1741 gggttgcagc agcagggcga cgccaccgaa gccgatgacg atggccagca gcagggcagg
     1801 cctggcttcg ccgcgcaggt agatcagcga taatgccgcg agaaacaggg gcgaggtgta
     1861 attgagcgtg accgcggtag ccagcggcaa tttgccgatg gcgtagaaga acagcaccag
     1921 cgccgcgaag ccgaacaggc tgcgcgacag gtgcggcttc cagtggcgcg tggcgagcct
     1981 tttgcggccg gcgaggctga ggaggaggat ggccagcagc ccgaacagcg agcggtagaa
     2041 caccagttcg gcgctggtga atttttgcgc gccgatcttg accagcgccc ccatgcaggc
     2101 aaagaacacg ccggctgcca gcatccacag gctgccgtag gtggtgggtt tcatcagcaa
     2161 ggccggcgcc gggctatttc aggtgcggct cgagctgcgc gcgcatccac aggtggaaat
     2221 gctccatgcc ggtttccatg gggtgctggt aggggccgta ttcatccagg tcttgtgcga
     2281 acagggcttg ccgcccgtcg tgcatgcgct ggcagatgtc ctcgtcctca atggcggttt
     2341 cggtgtaggc ggcctgctcg gcctcgatga actcccgttc gaacagcgcg atgtcttccg
     2401 ggtaatagaa ctcgaccacg ttggtgcagg cgttggggcc gcgcgggatg atattggaga
     2461 ccaccagcac gtgcgggtac cactcgatca tcaggaacgg gtagtacacc atccagatgg
     2521 cgccgtattt cggcagctcg ccgttgccat agcgcagcac ctgttcctgc cagcgctggt
     2581 atgccggcga gccggatttc tgcagggcgt ggttgatgcc gacggactgc acgctgtacc
     2641 actcgccgaa ttcccacttg aggtcgtcgc agtcgacgaa attgccgagg ccggggtgga
     2701 agggcgcgac gtggtagtcc tccaggtaga cctcgatgaa ggtcttccag ttgaactggt
     2761 attcgtcgac catcacgcgg tcgagcatga agccgctgaa gtcgaaatcc ttgaggaagc
     2821 cgagcttgcc caggtcgcga gcaacatcgc gccggctgtc gaaaagcagg ccgttccagt
     2881 tttgcagcgg ggtcttggac aggttgaggc agggctgctg ctcgaagtgc ggcgcgccca
     2941 tcagttcgcc ttgcaggtcg taggtccaac gatgcagcgg gcagacgatg ttttgggcat
     3001 ttcccttgcc tttcagcatc agggcctggc ggtgacggca gacgttgctg atgagctcga
     3061 tgccgctgtc gttgcgtacc agtgccttgg cgtggttcat ccattccagc gcgtgatagt
     3121 ctccgggatt gggcaccgtc agcgcgtggc cgacgtaagc cggcgcgtgc ggaaacagcg
     3181 cctgacgttc gagttcgtag atcgcgggat cgacatacca ggaaacaggc aactgggccc
     3241 ggggctggcg tttgatgggc gtgatttgag caagtgacat aatttcgagc gtcctccgaa
     3301 ttgctggccg aggaacatga tcaggcctta cgcctgaatt tcaattagag gcggtatttt
     3361 gccccaacct gcaatttttt caaaacttgc atatcgagag gtttcgagtc catggtcagg
     3421 tatgccatcg caaacgcttg catatggctt ttgccgaggc gtcattgctt gcccgtacgg
     3481 gcgatttgcg ataaccttgc cgcttcaacg agttggcaaa aaaactgggg gcgatcatga
     3541 aaaatccgct ggactccatc tccggaacca ttgtttccgg cctcgtgctg acgctcgtcc
     3601 tgtatttctt tctgaaaaac ttcattctgg cgggggtgta gatcatggaa ttcgcgcctg
     3661 caatcgcccg ctggatacat ttgctggccg gggttgcctg ggtcggcctg ctgttctatt
     3721 tcaacctggt gcaggtgccg gcattgaagg ccgccgcagc tgacggcact gccgccggca
     3781 ttaccaggca cgtcgccccg cgcgcactgc tgtggttccg ctgggctgcc gtggtaacct
     3841 ggctggccgg agcctctctg cttggcgcga atttcaccgc ggcctttact ttgcggccgg
     3901 actttgcgcc gatcggcatc ggtgcctggc tgggcaccat catgctgttc aatgtgtggg
     3961 tgctgatctg gccgaaccag cagaaagtgc tggggatgaa gccagcgagc gacgaagaga
     4021 agaacagggc gcgacgtgta gcgttgctgg cctcgcgaac caacatgatg ctgtcgatcc
     4081 cgatgctgtt tttcatggct ggcggcttga cacatcgagc tgcattcggc ttctagcaaa
     4141 ggttctgact attcggcaaa cggcggcgtt ggccgccgtt cttgtttttt ggaattgagt
     4201 aaaccatgtc acaacatctg atgaacacct acggcagaca gccggtcacc tttgtcaaag
     4261 gcgagggcgt atggctgtgg gatcagcaag gcaagcgcta tctggatggt ctggctgggg
     4321 tcgcagtgaa cggtctcgga catgcccacc caaggctgac ggctgccatc tgcgaacagg
     4381 cttcccgcct gatccatgtt tccaatattt accgcatcga cgagcaggcc aagttggcag
     4441 atcgcctggc ggaaatctcc ggcatggacc ggattttctt ctgcaactcc ggctgcgagg
     4501 ccaacgaggc ggcgatcaag ctggcacgcc tgtatggcca cagcaaggac attgagcacc
     4561 cggaaatcgt ggtcatggac aaagccttcc atggccggac attggcgacg ctgtcggcca
     4621 ccggcaaccg caaggtgcag gccggtttcg agccgctggt gccgggcttc gtgcgcgtgc
     4681 cgttcgatga catggacgca gtacgccagg tggccaggca caaccccaac gtggtggcgg
     4741 tgctggtgga accggtgcag ggcgagggcg gcatcaatat ccctcatgac ggcgccggct
     4801 acctgcgcgg cctgcgcgag atttgcgacg ccaacgactg gctgctgatg ctggacgagg
     4861 tgcaaagcgg catcggccgt accggcacct ggtttgcctt tcagcatgcc ggcatcgttc
     4921 ccgacgtgat gacgctggcc aagggcctgg gctcgggcgt gccgatcggc gcctgtctgg
     4981 ccaggggcgc cgcagccgag gtgtttaccc cgggcaagca cggctcgacc ttcggcggca
     5041 atcctctggc ctgcgccgcc gcgctggcga cgctggacgc catcgagcag gaaaagctgc
     5101 gcgagaatgc cgagactatc ggaaacttta tatgcgacgg cttccgccaa gcctttgccg
     5161 gggttgccgg ggtggtgacg attcgcaacg ccggcttgat gatcggcatc gagttggatc
     5221 gcccatgtgg cgaactggtg aagcaggcgc tggaagcagg gctgctgatc aacgtcaccg
     5281 ccgacacggt ggtgcgcctg ctgccgccac tggtgatgaa ccgggaggag gcgcagcagc
     5341 tggtcgacat cctggcgccg ctggtgaaag gctttttggg tcgttaatcc tgacgaatat
     5401 tacttatggc catcaaacac tatttgcaat tcaaggattt gaaccgcgac gagctggatt
     5461 acctgttcga gcgcacgcgc tggatcaagc agcagttcaa gtcgtaccag caatactggc
     5521 cgctgacaga tcgtactttg gtgatgatct tcgagaaggc cagcacgcgc acccgcctgt
     5581 cgttcgaggc cggaatccag cagctgggcg gctcggccat ctacctcaac acgcgcgatt
     5641 cgcaactcgg gcgcggcgaa ccggtggagg acgcggccca ggtgatttcg cgcatgtcgg
     5701 acatcgtcat gatccgcacc ttcgagcagg agatcatcga gcgctttgcc tcgcattcgc
     5761 gcgtgccggt gatcaacggc ctgaccaacg aataccaccc gtgccagatc ctggccgata
     5821 tcttcaccta catcgagcat cgcggcccga ttcagggcaa gacggtggcc tggatcggcg
     5881 attccaataa cgtctgcaac acctggctgc aggcggcgga ggtgctggat ttcaacgtgc
     5941 acgtttccac gccgccgggc tacgaggtgg aagcggaacg cgccggactg tacggccacg
     6001 accattacga ggagtttgcc gatcccatgg aagccgcgca aggcgccgat ctggtgacca
     6061 ccgacgtgtg gacctcgatg ggcttcgagg cggaaaacga agagcgcctg cgcgacttcg
     6121 ctgactggca ggtcgatgcc gacatgatgc gcgtggcagc caaggatgcc gtgttcatgc
     6181 attgcctgcc ggcgcaccgc ggcgaggaag tcgcggcgga agtcatcgac gggccgcaga
     6241 gcgtggtgtg ggatgaagca gagaaccgcc tgcacgtgca gaaggcgttg atggaatatt
     6301 tattgttagg aaaagtgagt caatgagcca aatcaacaaa gtcgtgctcg cctactccgg
     6361 cgggctggat acctcggtta tcctcaagtg gttgcaggat acctaccagt gcgaggtggt
     6421 gacctttacc gccgacatcg gccagggtga agaactggaa ccggcgcgtg ccaaggcgtt
     6481 gaaattcggc atcaagccgg agcagatcta catcgacgac ctgcgcgaag agttcgtgcg
     6541 cgatttcgtg ttcccgatgt tccgcgccaa cacggtgtat gaaggcgaat acctgctcgg
     6601 cacctccatc gcccgtccgc tgatcgccaa gcgcctcatc gaaatcgccc gcgaaaccag
     6661 tgccgacgcc atttctcacg gcgccaccgg caagggcaac gaccaggtgc gcttcgagct
     6721 cggcgcttat gcgctcaacc ctaatatcaa ggtcatcgcg ccgtggcgcg aatgggatct
     6781 gctgagccgc gaaaagctga tggcctatgc cgaaacccac ggcatcccgg ttgacatgaa
     6841 gcataagcag ggcggttcgc cgtattccat ggatgccaac ctgctgcaca tctcctacga
     6901 gggccgccat ctggaagacc cggcggccga ggccgaggaa tccatgtggc gctggacggt
     6961 cagcccggaa caggcgcctg atgccccgga atatctggat atcgaatacg tcaacggcga
     7021 cccggttgcg ctgaacggca agaagctggc cccgcacgca ctgctggccg agctcaaccg
     7081 cctgggcggc aagcacggca tcggccgcct cgacctggtg gaaaaccgtt acgtcggcat
     7141 gaagtcgcgc ggctgttacg aaactcccgg cggcaccatc atgctcaagg cacaccgcgc
     7201 catcgagtcg ctgaccctgg accgtgaagt cgcgcacttg aaagatgatt taatgccgcg
     7261 ctacgccagc ctgatctata acggctactg gtggagcccg gaacgcgttg cgctgcaaac
     7321 cctgatcgac cacacgcaga agagcgtcaa cggcttcgtg cgggtcaagc tatacaaggg
     7381 caacgtgatc gtggtcggcc gcgacagcaa gaccgattcg ctgttcgatc cgaccatcgc
     7441 caccttcgag gacgatgccg gcg
//
LOCUS       DAOMFA010000076         7404 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_1206_length_7404_cov_5.131583, whole genome shotgun sequence.
ACCESSION   DAOMFA010000076 DAOMFA010000000
VERSION     DAOMFA010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 7404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7404
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1206_length_7404_cov_5.131583"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..467
                     /locus_tag="PKW44_05145"
     CDS             <1..467
                     /locus_tag="PKW44_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Asd/ArgC dimerization domain-containing protein"
                     /protein_id="HQR51004.1"
                     /translation="TPILFAEAGDNFKAYGVAGHRHLPEISQGLAAMSGQKVGLTFVP
                     HLTPLIRGIHATLYARLQKDVDLQQLFEQRYQAERFVDVLPAGAHPETRSVRGYNQCR
                     IAVHRPQGGDTVVVLSVIDNLVKGAAGQAVQNMNIMFSFAENAGLELVPLLP"
     gene            560..1171
                     /locus_tag="PKW44_05150"
     CDS             560..1171
                     /locus_tag="PKW44_05150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR51005.1"
                     /translation="MALLLLGLGYGLGYWNHAGSNRNQLEQTMQRLQAENQAMQAGIA
                     QVERRLQVEHAAQSNLAREMAALQDEGIRLKEDVAFYQSILAESGATGVPRLHSVKLS
                     KGIHAGEYQYRILLVQSGRHDRTVQGNLLLLLNATQSGRPITERIEPAGQGKEIKVDF
                     KYFQRIEGTFSVPSGMDAQSVQVQFTETGGAKAKLSQTVNLPV"
     gene            1195..1611
                     /locus_tag="PKW44_05155"
     CDS             1195..1611
                     /locus_tag="PKW44_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HQR51006.1"
                     /translation="MFNRNSHKPQNRIDTLIGAEAVVEGNVSFSGGLRVDGCIRGNVN
                     EVGAKAGTLVLSEHGRIEGAINVSHVVINGIVQGPVSARAYIELQAKARVTGDVCYKT
                     LEIHMGGVVEGKLIYLAESQQGTTGEVYASKIDPLA"
     gene            1653..2003
                     /gene="erpA"
                     /locus_tag="PKW44_05160"
     CDS             1653..2003
                     /gene="erpA"
                     /locus_tag="PKW44_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HQR51007.1"
                     /translation="MNAVNEIPSPLIFTDNAAKKVKELIDEEGSPDLKLRVFVTGGGC
                     SGFQYGFTFEDTVNEDDTSVDRDGVTLLIDPMSLQYLVGAEIDYQDSLQGSQFVIRNP
                     NATTTCGCGSSFSA"
     gene            complement(2061..3353)
                     /gene="gltA"
                     /locus_tag="PKW44_05165"
     CDS             complement(2061..3353)
                     /gene="gltA"
                     /locus_tag="PKW44_05165"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019896524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HQR51008.1"
                     /translation="MKPTKATLTFDNGEKPIELPVLSGNLGADVIDISSLGKHGYFTY
                     DPGFLSTASCGSGVTYIDGDKGLLYYRGYPIEQLAEHCDFPEVAYLLINGELPNAEQK
                     QQFTDTVHSHTMLHDQMSNVFRGFRRDAHPMAVMVSVVGAMAAFYPENSDVSDPKQRE
                     IAAFRLLAKVPTVAAWSYKYNIGEPFAYPQNRLNYAENFLHMLFATPCESYTPNPVLA
                     NALERILILHADHEQNASTSTVRVVGSSGANPYACIAAGIASLWGPLHGGANEATLQM
                     LEEIGDVVRIGNYIKRAKDKTDSFRLMGFGHRVYRNMDPRAKIMRQTCHQVLDELGLH
                     DDPMFKLALKLEQIALEDEYFVSRRLYPNVDFYSGIVMRAMGIPNSMFTAIFALARTA
                     GWIAQWSEMKSSPEHRIARPRQLYTGEVRREFVPLVQR"
     gene            complement(3478..4569)
                     /locus_tag="PKW44_05170"
     CDS             complement(3478..4569)
                     /locus_tag="PKW44_05170"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HQR51009.1"
                     /translation="MTRYIGLMSGTSLDGVDATLVSFCPSGAARLEAQAYLPFPDELQ
                     QHLLALNNPGENEIERGCVLANRLAKLYAESVAILLQQTGLKATDIAAIGSHGQTIRH
                     RPDLGFTVQIGNSALLAELSGIAVVSDFRSRDIAAGGQGAPLVPAFHQAVFSHPAIHR
                     VIVNIGGISNLTDLPPGGVVTGFDCGPGNLLMNAWTQRHTGQNYDLGGAWAASGSLIP
                     ALLQALLSHPFLRQAPPKSTGRDTFHTAWLNALLQAEYAPVDVQATLLEYSARCIGDA
                     IDQYCTTATEIYLCGGGAHNHALCMRLQQLLGSRTVMLTDNLGIDADWVEAAAFAWLA
                     RQTLLGQPGNLPTATGARGLRIVGAIYPG"
     gene            complement(4589..5923)
                     /locus_tag="PKW44_05175"
     CDS             complement(4589..5923)
                     /locus_tag="PKW44_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HQR51010.1"
                     /translation="MQQGKSGILAQNPIAQERKLRLRWLLGVSSIPLFGIITAFGIAP
                     QTADQNIPVATVVENISLPAQSAAIDTAIPMNHSYWQADQVRKDDTLGSLLERLNIRN
                     NDAIDFLRHASEARGLASQLRPGRNILAKTSEDGTLHELHYQLSADTSMSVKLIDGVY
                     QAEQNQIALENRTQMGSAEIHSSLFAATDDAGIPDSVAMQLVEIFSSDIDFHLDLRKG
                     DHFSVVYEGSYNNGELVKAGRVLAAEFINDGNTYQAVLYQGEDGQSNYYTPNGKSLHK
                     AFLRSPLEFSRISSGFSLARFHPVLQKFRAHKGVDYAAPTGTGVKATADGTVAFVGTQ
                     GGYGNVIMLQHQGGATTVYGHLSRFAPGLRNGQKIAQGEIIGQVGMSGMATGPHLHYE
                     FRVNGVHRDPLKVALPNVPPIAAHNLAAFQQQAQPRLSQLALLRGALTASLD"
     gene            5999..7198
                     /gene="tyrS"
                     /locus_tag="PKW44_05180"
     CDS             5999..7198
                     /gene="tyrS"
                     /locus_tag="PKW44_05180"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HQR51011.1"
                     /translation="MSQMNDALAKIKRGCDELLIEQELVDKLKAGRPLRVKAGFDPTA
                     PDLHLGHTVLLNKLRQLQDLGHHVLFLIGDFTGMIGDPTGKSATRPPLTQEQVQENAK
                     SYQDQVFKVLKPEQTEVVFNSAWMGKLDAAAMIRLAATHTVARMLERDDFNKRYRNNQ
                     PIAIHEFLYPLLQGYDSVEIKADLELGGTDQKFNLLMGRELQKHFGQAPQCILTMPLL
                     EGLDGVNKMSKSLGNYIGIAESSQEIFGKLMSVSDELMWRYIELLSFESLETIRQWKG
                     EVEAGANPRNIKVRFAQEIVERFHSRAAAEQALADFEARFQRGAIPDDMVEVSVQCQG
                     GELMVTQMLKQAGLVASTSEAVRMIEQGGVKLDGEKVSDKGLKLLSGAVVVAQVGKRK
                     FARVTLI"
BASE COUNT         1592 a         1951 c         2259 g         1602 t
ORIGIN      
        1 agacccctat tctgtttgcc gaggccggtg acaatttcaa ggcctacggc gtggccggac
       61 atcgccattt gccggaaatt agtcaagggc tggcggcgat gtccggccaa aaagtcgggc
      121 tgacgttcgt gccgcacctg acgccgctga ttcgcggtat tcatgctacc ttgtatgcgc
      181 ggctgcaaaa ggacgttgac ctgcagcagc tgttcgagca acgctatcaa gcggagcgct
      241 tcgtggatgt gttgcccgcc ggcgcacatc cagaaacgcg ctcggtgcgc ggctacaacc
      301 agtgccgcat cgccgtgcat cggccgcagg gcggtgatac cgtggtggtg ttgtcggtga
      361 tcgataacct ggtcaagggc gctgcaggac aggcggtgca aaacatgaat atcatgttca
      421 gttttgctga gaacgccgga ctggagctgg tgccgctgtt gccatgaggc ggcttcggcg
      481 cggtctgaga cgccactccg gcatcgctgc acggcgtgtc tccgtccgca ccgcgtggcc
      541 ttggcaatgg cgtctggcaa tggcgctgtt gctgcttggg ctgggctacg gtttaggcta
      601 ctggaaccat gccggcagca acaggaatca gctggagcag acgatgcagc gcctgcaggc
      661 ggaaaatcag gcaatgcagg caggcatagc acaggtggag cgtcggctgc aggttgagca
      721 cgccgcgcaa agcaacctgg cgcgagaaat ggcggcgcta caagacgaag gcatacgcct
      781 gaaagaggat gtcgccttct accagagcat tctggccgag agcggcgcca ctggcgtacc
      841 caggctgcat agcgtcaagc tgagcaaggg catccatgcc ggtgaatacc agtaccgtat
      901 actgttggtt cagtccggcc gacacgacag gacggtgcag ggaaacctgc ttttgctact
      961 gaacgcgacc cagtccggaa ggccgataac cgaacgtatt gaacccgccg gccaaggcaa
     1021 ggaaatcaag gtggatttca agtatttcca gcggattgaa ggaacgttca gtgtaccgtc
     1081 agggatggac gcacaatccg tgcaggtgca gttcaccgaa acgggcgggg caaaggccaa
     1141 gctttcacaa accgtcaatc tgccggtctg attgattttt agtctagggt agccatgttt
     1201 aacagaaaca gtcacaaacc acagaaccgt atcgacaccc tgatcggcgc cgaagccgtc
     1261 gttgagggta acgtcagttt cagcggaggg ctgcgcgtgg atggctgcat ccgcggcaac
     1321 gtcaacgaag tcggcgccaa ggcggggaca ctggtactga gcgagcatgg ccgtatcgaa
     1381 ggcgcgatca atgtttccca tgtggtcatc aacggcattg tgcaggggcc ggtgtccgcg
     1441 cgcgcttaca ttgaacttca ggccaaagcc cgggtgactg gcgatgtctg ctacaagacc
     1501 ttggaaatcc atatgggcgg ggtggtggaa ggcaagttga tttatcttgc tgaatcgcag
     1561 cagggaacta caggcgaggt ttacgcatca aagattgacc cgcttgcctg agccgcggat
     1621 aatggttcgc ctaaagtgtt aaggagttgg aaatgaatgc tgtcaatgaa atccccagcc
     1681 cgctgatctt taccgacaac gcggcgaaaa aagtgaaaga gctgattgat gaagaaggct
     1741 cgccggatct gaagctacgc gtattcgtca ctggcggcgg ctgttcaggt ttccagtacg
     1801 ggttcacttt tgaagacacg gtaaacgagg atgacacctc agtagatcgt gacggtgtga
     1861 ccctgctgat cgacccgatg agcctgcaat atcttgtagg cgcagaaatc gattatcagg
     1921 acagcttgca aggttcgcag tttgtcatcc gcaacccgaa cgctacgacc acttgcggtt
     1981 gcgggtcttc tttctccgcg taaccgccgg agtcggcaac aaaaaaggag gctcatcgcc
     2041 tccttttttg cttctggtgc ttagcgctgt acgagaggca cgaattcgcg gcggacttcg
     2101 ccggtatata actggcgcgg gcgtgcgatg cggtgttccg ggctggattt catttcactc
     2161 cactgcgcga tccagcctgc agtacgtgcc agcgcgaaga tggcggtgaa catcgagttt
     2221 ggaatcccca tggcacgcat tacgatcccg gagtagaaat ccacattggg atagagccgc
     2281 ctgctgacga aatattcgtc ttccagcgca atctgttcca gcttgagcgc cagcttgaac
     2341 atcgggtcgt catgcagacc aagctcatcc agcacctggt ggcaggtctg gcgcatgatc
     2401 ttggcgcgtg gatccatatt tcggtaaacg cgatggccaa accccatcag acggaacgag
     2461 tcggtcttgt ccttggcgcg cttgatgtag ttgccgatgc gcacaacatc gccgatttcc
     2521 tccagcattt gcagcgtagc ctcgttggcg ccgccatgta gcggccccca caatgacgcg
     2581 atgccagcgg cgatgcaggc gtaaggattg gcgccactgg aacccaccac acgaactgtg
     2641 gaggtggaag cattctgctc atggtcggca tgtagaatca ggatgcgttc cagcgcattc
     2701 gccagtacag ggttgggggt atacgattcg cacggtgtgg cgaacagcat gtgcaggaag
     2761 ttttccgcgt agttgagacg gttctgcgga taggcaaacg gttcgccaat gttgtacttg
     2821 tagctccatg cggcgaccgt tggtaccttg gccagcaggc ggaacgccgc gatttcacgc
     2881 tgcttcgggt cggaaacatc agaattttcc gggtagaacg cggccattgc gcccactacc
     2941 gacaccatca ccgccatcgg atgcgcgtcg cggcgaaagc cgcggaatac gttgctcatc
     3001 tggtcgtgaa gcatggtatg gctgtgcacg gtatcggtga actgctgctt ctgttccgca
     3061 tttggcagtt ccccgttgat cagcagataa gccacttccg ggaaatcgca gtgctccgcc
     3121 aattgctcga tcgggtagcc acggtaatag agcaggccct tgtcgccgtc gatgtaggtg
     3181 acgccagagc cgcagcttgc tgtggacagg aagccggggt cataggtgaa atagccatgc
     3241 ttgccaaggc tgctgatgtc gatcacatcc gcgccgagat tgccggacag caccggcaac
     3301 tcaatgggtt tttctccgtt gtcgaaagtc agggtggctt tggtcggctt catagggtga
     3361 ttttgcaatg aatcgttaga ggggttaata ctatgggctg ttgcagaatg tggcaagtga
     3421 ttcgcgggtg gcctgcatgt caaaaggggc agattgattt accccctgta gtgctactta
     3481 accgggatat atcgcaccca ctattcgcag ccctctggcg ccggtagctg tcggcaggtt
     3541 gcctggttgg cccagcaagg tttgcctggc cagccaggcg aaggcggcag cttcgaccca
     3601 gtccgcatct atcccaagat tgtcggtcag catgactgtc cttgaaccca gcaattgctg
     3661 caggcgcatg catagcgcgt gattatgggc gccgccaccg cataggtaaa tttccgtcgc
     3721 ggtcgtgcag tactggtcga tagcgtcacc gatgcagcgt gccgaatatt ccagtagcgt
     3781 cgcctgtacg tccacgggcg catattccgc ctgcagcagt gcgttcagcc aagccgtgtg
     3841 aaaagtgtcg cgcccggtgc ttttcggcgg ggcttggcgc aggaacgggt gtgacaacag
     3901 agcctgcagc agggctggga taagactgcc ggaggccgcc caagcaccgc ccaggtcata
     3961 attttgtccg gtatgccgct gtgtccaggc attcatcagc agattgccgg ggccgcagtc
     4021 gaagcctgtc acgacgccac ccggcggcag atcggtgagg ttgctgatac ccccgatgtt
     4081 gacaatgaca cggtggatcg cgggatggct gaaaactgcc tgatgaaaag ctggcacaag
     4141 tggcgcaccc tgcccgccgg cggcgatatc gcggctgcgg aaatcggaaa ctacggcaat
     4201 gccgctcaat tcggctaaca aggcggagtt gccaatctgc acggtaaaac cgaggtcggg
     4261 acggtgccga atggtttgcc cgtggctgcc gatggcagca atatccgtag ccttgaggcc
     4321 ggtttgttgc agcagtatag ctacgctttc ggcataaagt ttggcgagtc gattggccag
     4381 cacgcaaccg cgctcgattt cattctcacc ggggttgttg agagcgagta aatgctgttg
     4441 cagctcatcc gggaagggca ggtaggcctg agcttcaagt cgtgcggcac ctgatggaca
     4501 gaaggaaacc agcgtggcat caacgccgtc cagactggtg ccggacatca gcccgatgta
     4561 acgggtcaat tctggtgagg ggttgttgtt agtcaagcga cgcggttagg gcgccgcgca
     4621 acaatgctag ctgggagagg cgcggttgag cctgctgttg gaacgctgcg agattgtgcg
     4681 ccgcaatcgg cggcacgttt ggcaatgcga ccttgagcgg atcacggtgt acgccattga
     4741 cgcggaattc gtaatgcagg tgcgggccgg tcgccatacc gctcatgcca acctgaccaa
     4801 taatttctcc ctgcgcaatt ttctgaccgt tgcgtagccc tggcgcaaac cgcgacaaat
     4861 ggccataaac ggtagtggcg ccaccctgat gttgcagcat gatcacgttg ccgtaacctc
     4921 cctgggtgcc gacgaaagcc acggtgccat ccgcggttgc cttgacgccg gtaccggtag
     4981 gcgcggcata gtccacaccc ttgtgcgcac gaaatttttg cagaacggga tgaaagcgtg
     5041 ccagcgaaaa gccggaactg atacgtgaaa actccagcgg agaacgcaga aaagccttgt
     5101 gcaagctctt gccgttaggt gtgtagtaat ttgactggcc gtcctcgcct tggtagagca
     5161 cggcctgata ggtgttgcca tcattgataa attcggctgc cagcacccgt ccagctttga
     5221 ctagttcgcc gttgttgtag ctaccttcat acacgacgga gaagtgatcg cccttgcgca
     5281 ggtcgaggtg aaaatcgata tcggaagaaa agatttcgac cagttgcatg gccacactat
     5341 ctgggatgcc ggcatcgtcc gtggcggcaa acagcgagct gtggatttcg gccgatccca
     5401 tctgggtgcg gttttccaga gcgatctggt tttgctcggc ttggtaaacg ccatcaatca
     5461 gcttgacgct catcgaggtg tcggcgctta gctggtagtg caattcgtgt agcgtgccgt
     5521 cttcactggt ttttgccaga atgttgcgtc ctggccgtaa ttgcgaggcc agcccacgcg
     5581 cttccgacgc atggcgcagg aagtcgatgg catcgttgtt acggatgttg aggcgctcca
     5641 gtaagctgcc cagcgtgtcg tccttgcgca cttggtcggc ttgccagtag gaatgattca
     5701 tcgggatggc ggtatcgatg gctgcagatt gtgccggcag gctaatgttt tccacgacgg
     5761 tggcaaccgg gatgttctga tcagcggtct gtggtgcaat gccgaaggcg gtgatgatac
     5821 cgaacagcgg gatggaggaa acgccaagca gccaacgcag gcgcagcttt cgttcttggg
     5881 ctatcggatt ttgcgctaaa atgccgcttt ttccttgttg cacaggcgtt ccttagaaga
     5941 tatttcttac gacccgcacg atgctaacac aaaactttaa aattggtgaa ggcttgccat
     6001 gtcacaaatg aatgatgctc tggctaaaat taaacgcggc tgtgacgaat tgctgattga
     6061 gcaggaattg gtcgataagc tcaaagccgg tcggccattg cgggtcaagg ccgggtttga
     6121 tcccaccgcg ccggacttgc atcttggcca cacggtatta ctcaacaaat tgcggcagtt
     6181 gcaggatctt ggccatcatg tgctgttcct gatcggtgac tttactggca tgatcggcga
     6241 ccccaccgga aaaagcgcta cccgtccgcc gctcacccag gagcaggtgc aggaaaatgc
     6301 caagtcctac caggatcaag tgttcaaggt tctcaagccc gagcagaccg aggtagtgtt
     6361 caattcagcc tggatgggca aactggatgc cgctgccatg atccggctgg ctgccactca
     6421 tactgtcgcc cgcatgctgg agcgagacga cttcaacaag cgctatcgca acaaccagcc
     6481 gatcgcgatt catgaatttc tttatccttt attacaaggg tacgactcgg tcgaaatcaa
     6541 ggccgacctg gagctgggcg gcaccgacca gaaattcaac ctgctgatgg ggcgggaatt
     6601 gcagaagcat ttcggccaag cgccgcaatg tatcctgacc atgccgctgc tggaagggct
     6661 ggatggcgtc aacaagatgt ccaagtcgct gggcaactac atcggcatcg ctgaatcgtc
     6721 gcaggaaatt ttcggcaagc tgatgtcagt ttctgatgag ctgatgtggc gctatatcga
     6781 actgctgtcg ttcgaatctc tggaaaccat ccgccaatgg aaaggcgagg tcgaagccgg
     6841 cgccaatcca cgcaacatca aagtgcgttt tgcgcaggag attgtggagc gatttcattc
     6901 aagggcggct gccgaacaag ctttggcgga ttttgaggcg cgtttccaga gaggcgctat
     6961 tccggatgac atggtcgagg tcagcgtcca gtgccaaggt ggtgagttga tggtcacgca
     7021 aatgcttaag caggcgggcc tggtggccag tacttccgaa gcggtgcgca tgatcgagca
     7081 gggcggcgtc aagctcgatg gtgagaaagt tagcgacaag gggttaaagc tgctgtccgg
     7141 cgcagttgtt gttgcgcagg tgggcaagcg caaatttgcc cgcgtcaccc ttatatagat
     7201 agaaggtggg caacggggtg cggggaattt tgttgcgcca cctcttgacc gttctgccac
     7261 gatctgtaga atgcgcgcct tcgctgtgaa ggcgaatctg ctgccgggta acaaatttac
     7321 atgttccccc ttgaccgact gaaatcggcc tgtataatgc gcggctctcg ttactttggt
     7381 agcggggttt gaagtaactg ctct
//
LOCUS       DAOMFA010000077         7285 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_1231_length_7285_cov_5.991010, whole genome shotgun sequence.
ACCESSION   DAOMFA010000077 DAOMFA010000000
VERSION     DAOMFA010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 7285)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7285)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7285
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1231_length_7285_cov_5.991010"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..639
                     /gene="recO"
                     /locus_tag="PKW44_05185"
     CDS             <1..639
                     /gene="recO"
                     /locus_tag="PKW44_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HQR51012.1"
                     /translation="VELFSRNVGRVAAVANGARRPRSAMRGMLQSFQPLLAAWSGKSE
                     LRNLHTLDWGEGLLLLQGEALMCGFYLNELLLRLLPREDPHEALYDYYAQALRQLAAG
                     DPAAPTLRRLELRLLQELGYAVPLEQQPDGTQVEAGRDYCYLPEHGALPPEGKETGVQ
                     LTGKTLLDMACDDYSDALTLQQSKQLMRTLLAHYLGDKPLHTRQLLIDLQEL"
     gene            636..1367
                     /gene="pdxJ"
                     /locus_tag="PKW44_05190"
     CDS             636..1367
                     /gene="pdxJ"
                     /locus_tag="PKW44_05190"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HQR51013.1"
                     /translation="MIRLGVNIDHVATIRQARGTRYPSVVQAALRAEQAGADSITLHL
                     REDRRHIQDVDVHTLRSLLQTRMNLEMAVTDEMVDIALQVRPQDVCLVPERREELTTE
                     GGLDVIGHFEKVQAACRRLRDAGIRVSLFIDPDEAQLEASKAAGASVVELHTGCFADA
                     EDDATQEQELGRIKNAVVHGVSLGLRVNAGHGLHYHNVHQIAAIPGVEELNIGHAIVA
                     HALFVGWDAAVREMKALMVVAAPGR"
     gene            1364..1741
                     /gene="acpS"
                     /locus_tag="PKW44_05195"
     CDS             1364..1741
                     /gene="acpS"
                     /locus_tag="PKW44_05195"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HQR51014.1"
                     /translation="MIHGIGTDIVEVSRIEESLVRFGETFAQRILSEREWLDFEQSQI
                     KARFLAKRFAAKEAFAKALGTGLRAPATFGNIGIAHDDLGKPVFDLAPELQACLEQRS
                     ICACHLSISDEKALAVAFVVLEA"
     gene            1738..2508
                     /gene="tcdA"
                     /locus_tag="PKW44_05200"
     CDS             1738..2508
                     /gene="tcdA"
                     /locus_tag="PKW44_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HQR51015.1"
                     /translation="MTQEADFERRFGGIRRLYGNHALERFRQAHVCVVGVGGVGSWAA
                     EALARSAVGRITLIDLDNVAESNVNRQIHALGNEFGKAKVTAMAERIHAINLQCQVTQ
                     VEDFVTPDNLDAMLSGGYDFIIDAIDSAKTKAAMIAWCKRHKVKLIVSGGAGGRVDPS
                     RIQLADLSRTIQDPLLSRVRTLLRREFGFPKDPKKKFGVECVFSSEPLQQPKTGAACD
                     VDVGTQHPTGLNCAGYGSSMTVTATVALLAVASGLRRL"
     gene            2694..4121
                     /locus_tag="PKW44_05205"
     CDS             2694..4121
                     /locus_tag="PKW44_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HQR51016.1"
                     /translation="MTENISKAFDEMRAVGGETRAVYREFSDWLDGTNHAHLTQKTRE
                     AELLFRRVGITFNVYGEEAGAERLIPFDVIPRILSATEWQRLSAGAIQRVRALNAFLD
                     DLYHGQEIIKAGIMPAGILTNEMYRPEMQGVDVPGGIYAHIAGIDIVRTGENQFYVLE
                     DNLRTPSGVSYMLEDRKMMMRLFPELFRRYSVAPVEHYPQVLLNNLRAVAQPGVQDPT
                     VVLLTPGAYNSAYFEHAFLAQQMGIELVEGQDLFVRDDAVFMRTTEGPKRVDVIYRRI
                     DDDFLDPLAFNPKSMLGVQGLMSVYRNGGVTLANAIGTGVADDKATYTYVPEMVRFYL
                     GEEPILSNVPTYRLSIEDDRSYVLDHLQELVVKEVQGSGGYGMLVGPAASKAEVEEFR
                     ARIIADPDNYIAQPTLALSTCPTLVEQGVAPRHVDLRPFVLSGKTVTLVPGALCRVAL
                     REGSLVVNSSQGGGTKDTWVLNYAE"
     gene            4111..5058
                     /locus_tag="PKW44_05210"
     CDS             4111..5058
                     /locus_tag="PKW44_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HQR51017.1"
                     /translation="MLSRTADHLYWMARYIERAENMARVLDVTYRMSLVANCDHSEAD
                     LWKPPVLIADDVAAFERDYGDYNAANVMRYMALDERNQSSIVSCLGAARENARSVRVA
                     MSSEMWETVNALWLELRQRTAQDITEADIGEFCDWVKSRSHLFRGVTFGTMLRDDGYK
                     FVRLGSFIERADNTARLLDAKYHLLMAAGAGAEGAVDYYEWSSLLRSVSSFEAYQKVY
                     RDAIEPSKVAELLVLRDDMPRSLHACYDELTPILDQICIVRGYECLRQAGEIHARLHF
                     GRMEDIFNSGLHQFLQDFIIHNNTLGREIQHAFLNAPAD"
     gene            5253..6488
                     /locus_tag="PKW44_05215"
     CDS             5253..6488
                     /locus_tag="PKW44_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013146740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR51018.1"
                     /translation="MINSLIYPKQGDKANSPWFEEFLVRLLEDRDRIGLTEMIREIDA
                     MMITVEPGCSVAYVSELALMTPYHYLVTLESESHWTHVLRIDMNSPDILVREVRDPGK
                     HGIFRSLNEVYPIGAHKPNSRYMGEIFRVTNLHDVVELQKAREIRFFNQDQIRKLELP
                     GNMALVKPSPYTHNIVGYWERPPEDIRVYALGNSVIRDDVNRGYQEAKETQAKLGLDK
                     LILPIDHLATRVYSQNREVAILEYLTLSSYYYWGSYDIADQNSSTNVTKSIHYPEESI
                     SPAKEFTAANHPYFVRHLVGLPSPTESFVRNYGPRLHHIALSVADGEVEGKANIDYVV
                     DAIKAKGKDFLLDVIGSKEEGLKQIFSSASEHSSLIIEYVQRFGDFDGFFTKQNVAEL
                     TEAAGVEENLRLLQAESTA"
     gene            6485..>7285
                     /locus_tag="PKW44_05220"
     CDS             6485..>7285
                     /locus_tag="PKW44_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013146739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HQR51019.1"
                     /translation="MTIKVAIHHKTAYHYDHPVQLTPHVFRLRPATHSRTPISAYSLK
                     IKPEKHFINWQQDPFGNYLARVVFPEKSRELSIEVDMVAEMTVINPFDFFLEEYAEHF
                     PFSYPEQLQKELTPYLEVREDGPLLRQFLAGADHGKQGIVDFLVGINQRVNQTVNYSI
                     RMEPGVQTCEETLEKKLGSCRDSGWLLVQALRQMGLAARFVSGYLVQLVADVKSLDGP
                     SGTDKDFTDLHAWAEVYIPGAGWIGLDPTSGLFAGEGHIPLACTADYVS"
BASE COUNT         1470 a         2221 c         2195 g         1399 t
ORIGIN      
        1 gtcgaactgt tcagccggaa tgtcggccgt gttgcggcgg tggccaatgg tgcgcggcgt
       61 ccgcgctccg ccatgcgtgg catgctgcaa tcgttccagc cgctgctggc ggcgtggtcg
      121 ggaaagtccg agctgcgcaa tctgcataca ttggactggg gcgagggctt gctgctgttg
      181 cagggcgagg cgctgatgtg cggcttctac ctcaacgaac tgttgctgcg cctgctgccg
      241 cgtgaagacc ctcacgaagc cctgtacgac tactacgccc aggcactgcg ccagttggcg
      301 gcgggtgatc cggcagcccc cacgctgcgc cggctggagc tgcgcctgtt gcaggagctg
      361 ggctacgccg tgccgctgga gcaacagccg gatggcacgc aggtcgaggc cgggcgcgac
      421 tactgctacc tgccggagca tggtgcactt ccgccggagg gcaaggaaac gggtgtacaa
      481 ttgaccggaa aaaccctgct cgacatggcc tgcgacgact actccgatgc gctcaccctg
      541 cagcagagca agcagttgat gcgcaccttg ctggcgcatt acctcggcga caagccgctg
      601 catacccgac aactacttat cgacctacag gaactatgat cagactcggc gtcaacatcg
      661 accacgtcgc caccatccgc caggcgcgcg gcacgcgcta ccccagcgtg gtgcaggcag
      721 cgttgcgcgc ggaacaggcc ggcgcggatt ccatcacttt gcatctgcgc gaagaccgcc
      781 gtcacatcca ggatgtggac gtgcataccc tgcgcagctt gctgcaaact cgcatgaacc
      841 tggaaatggc ggtaaccgac gagatggtcg acatcgcgct gcaagtcaga ccgcaagatg
      901 tctgcctggt gcctgagcgg cgcgaagagc tgaccaccga gggcgggctg gacgtgatcg
      961 gccatttcga gaaagtacag gctgcctgcc gcaggctgcg tgatgccggc atccgggtat
     1021 cgctgttcat cgatcccgac gaggcgcaac tggaggcgtc caaggccgct ggcgcgtcgg
     1081 tggtggaact gcacactggt tgctttgccg acgccgagga cgatgcaacg caggagcagg
     1141 aactcgggcg catcaagaat gccgtggtgc atggcgtttc cctggggctg cgcgtcaatg
     1201 ccgggcacgg cctgcattac cacaacgtgc accagatcgc tgccatcccc ggcgtggaag
     1261 aactcaacat cggccatgcc atcgttgccc acgcactgtt cgtcggctgg gacgccgccg
     1321 tgcgcgagat gaaggcgctg atggtggtcg ccgctcccgg tcgatgatcc acggcatcgg
     1381 caccgatatc gtcgaagttt cccgcatcga ggaatcgctc gtccgcttcg gcgaaacttt
     1441 cgcgcagcgc atcctgagcg aacgcgaatg gctggatttc gagcaatcac agatcaaggc
     1501 cagattcctc gccaagcgct tcgctgccaa ggaggctttc gccaaggcgc tggggaccgg
     1561 cctgcgggcg ccggccacgt tcggcaacat cggcattgct cacgatgacc tgggtaagcc
     1621 ggtattcgac cttgcaccgg agttgcaggc ctgcctggag caacgcagta tttgcgcctg
     1681 ccacctttcc atcagcgacg aaaaagcgct ggcagtcgcg ttcgtcgttc tggaagcatg
     1741 acccaggaag ctgatttcga gcgccgcttc ggcggcattc gtcgcttgta cggaaaccat
     1801 gcgctggagc gcttccgtca ggcgcatgtg tgcgtagtcg gcgtcggtgg tgtcggttcc
     1861 tgggcggcgg aggcactcgc tcgcagcgcc gtcggccgca tcacgctgat cgatctcgac
     1921 aacgtcgccg agtccaacgt caaccgccag atccatgcgc tcggcaacga attcggcaag
     1981 gccaaggtga cggcgatggc cgagcgcatc cacgctatca accttcaatg ccaggtcacg
     2041 caggtcgagg atttcgttac cccggacaac ctcgacgcca tgttgagcgg cggctacgat
     2101 ttcatcatcg atgccatcga cagcgcaaaa accaaggccg ccatgatcgc ctggtgcaaa
     2161 cgccacaagg tcaagctgat tgtttccggt ggtgccggcg ggcgagtcga cccgagcaga
     2221 atccagttgg ccgatctctc caggaccatt caagacccgc tgctttcccg cgtgcgaacg
     2281 ctgctgcggc gagagttcgg ctttcccaag gatcccaaaa agaagttcgg tgtggagtgc
     2341 gtgttttcca gcgagccatt gcagcagccg aaaaccggcg ctgcctgcga tgtcgacgtc
     2401 ggcacgcaac accctaccgg cctcaactgc gccggctatg gttccagcat gaccgtcacc
     2461 gcgacagtgg cgctgctggc cgtggcaagt gggctgcgcc gactgtgaac tccaattgcc
     2521 cgctattagg gcaatggccg caacggtctc atgcagcctc aagatggggc accctgtcaa
     2581 tcaccgtgcc caaattcggt gcacaaacac atacgtaggg caaggatcgc tgttaacctg
     2641 ttgaaatacc atcatattga cgttggcatg accattgctg atatctattc atcatgaccg
     2701 aaaatatcag caaagcattc gacgagatgc gtgcggtcgg aggagaaacg cgggcggtat
     2761 accgcgagtt ttccgactgg ctggacggca ccaaccacgc gcatctcacg caaaaaaccc
     2821 gcgaggcgga actgctgttc cgccgcgtcg gcatcacttt caacgtctat ggcgaggagg
     2881 ccggcgccga gcgtttgatc ccgttcgacg tcattccgcg cattctgtcc gcgaccgaat
     2941 ggcagcgtct ttccgccggc gccatccagc gcgtgcgggc gcttaatgct tttctcgacg
     3001 acctttacca cgggcaggag atcatcaagg caggcattat gccggccggc atcctgacca
     3061 acgaaatgta ccgtccggaa atgcagggcg tggacgtgcc gggcggcatc tatgcgcaca
     3121 ttgccggcat cgacattgtg cgcaccggcg agaaccagtt ttacgtgctg gaagacaatc
     3181 tgcgtacgcc gtccggcgtt tcctacatgc tggaagaccg caagatgatg atgcggttgt
     3241 ttccggagtt gttccgtcgc tattcggtgg ccccggtcga gcactatccg caggtgctgc
     3301 tcaacaatct gcgcgccgtg gcccagcccg gcgtgcagga cccgaccgtg gtgctgctca
     3361 cccccggcgc ctataacagc gcctatttcg agcatgcctt cctggcgcaa cagatgggta
     3421 tcgaactggt ggaagggcag gatctgttcg tcagagacga cgcggtgttc atgcgtacta
     3481 ccgaaggccc caagcgcgtg gatgtgatct accgccgcat cgacgacgat tttctcgatc
     3541 cgctggcgtt caacccgaaa tccatgctgg gagtgcaggg cctgatgtcg gtctatcgca
     3601 acggaggggt cacattggcc aatgccatcg gcactggcgt ggcggacgac aaggcgacct
     3661 atacctatgt gccggagatg gtgcgcttct acctcggcga ggaacctatc ctttccaacg
     3721 ttccgaccta caggctgagc atcgaggacg atcgcagcta cgtgcttgat cacttgcaag
     3781 agctggtggt gaaggaggta cagggttccg gcggctacgg catgctggtc ggccccgccg
     3841 ccagcaaagc cgaggtcgag gaattccgcg cgcgcatcat cgccgatccg gacaactaca
     3901 tcgcccagcc tacgctggcg ctgtccacct gcccgacact ggtggagcag ggtgtggcgc
     3961 cgcgtcatgt tgacctgcgg ccatttgtgc tgtccggcaa gaccgtgacg ctggtgccgg
     4021 gcgcgctgtg ccgggtggcc ctgcgcgaag gttcgctggt agtcaattcc tcgcagggtg
     4081 ggggcaccaa ggatacctgg gtgttgaatt atgctgagta gaaccgccga tcacctctac
     4141 tggatggcgc gctacatcga gcgcgccgag aacatggcgc gcgtactgga cgtgacctac
     4201 cgcatgtcgc tggtggccaa ctgcgatcac agtgaggcag atctgtggaa gccgccggta
     4261 ctgatcgccg acgatgtggc cgcgttcgag cgcgactacg gtgactacaa tgccgccaac
     4321 gtgatgcgct acatggcgct ggacgagcgc aaccagtcca gcatcgtcag ttgcctcggt
     4381 gcggcgcgtg agaatgcgcg ctcggtgcgg gtagcgatgt cttctgaaat gtgggaaacg
     4441 gtcaacgcgt tgtggctgga actccggcag cgcaccgccc aggacataac ggaagcggat
     4501 atcggcgaat tctgcgactg ggtcaagtcg cgctcgcacc tgttccgcgg cgttaccttt
     4561 ggcaccatgc tgcgcgatga cggctacaag ttcgtgcggc tcggctcctt catcgagcgc
     4621 gccgacaaca ccgcccgcct gttggatgcc aaatatcatc tgctgatggc tgcgggcgcc
     4681 ggcgctgagg gtgcggtgga ttactacgaa tggagttcgc tgctgcgctc ggtgtcgtcg
     4741 ttcgaggcct atcagaaggt ctatcgcgac gccatcgaac cttccaaggt cgccgagttg
     4801 ctggtgctgc gcgacgacat gccgcgctcg ctgcacgctt gctacgatga actcacgccc
     4861 attctggatc agatctgcat cgtgcgtggt tacgagtgtc tgcgccaggc aggcgagatc
     4921 catgcgcgcc tgcatttcgg ccgcatggaa gacattttca acagcggcct gcaccagttc
     4981 ctgcaggatt tcattatcca caacaatacg ttgggcaggg aaattcagca tgccttcctc
     5041 aatgcgccgg cggactgaga tgcagcgaaa aaatggagtg caggcaatat cgcctgcaag
     5101 tggtcatcag gccgcattca caaacatgcg ggcagaggtg cttgcacacc cccctgaact
     5161 gttgcgacgt gccaaccagt ctgcaatggg gcaggatgat aaacggcatg ccatgcaaca
     5221 atcccaacaa taatctcttg taacgagtcg aaatgataaa ttcactgatt tatcccaagc
     5281 aaggcgacaa ggccaactcg ccctggttcg aagaatttct ggtcaggctg ctggaagacc
     5341 gcgatcgtat cgggcttacg gagatgatcc gggaaatcga cgcgatgatg attaccgtcg
     5401 aacccggctg ttcggtggcc tacgtcagtg aactggcgct gatgacgcct taccactacc
     5461 tggtcacgct ggaatcggag tcgcactgga cgcacgtgct gcgcatcgac atgaattcgc
     5521 cggacattct ggtgcgcgag gtgcgcgacc ccggcaagca cggtattttc cgcagcctca
     5581 acgaagtcta tccgatcggc gcgcacaagc ccaattcgcg ctacatgggc gagatcttcc
     5641 gcgtcaccaa cctgcacgac gtggtcgagt tgcaaaaggc gcgcgaaatc cgctttttca
     5701 atcaggacca gatccgcaag ctggaactgc cgggcaacat ggcgctggtg aaaccctcgc
     5761 cctacaccca caacatcgtc ggctactggg agcgcccgcc ggaagacatc cgggtctacg
     5821 cgctgggtaa cagcgtaatc cgcgacgacg tcaatcgcgg ctaccaggaa gccaaggaaa
     5881 cacaggccaa gctgggcctg gacaagctga tcctgcccat tgatcacctc gccacccgcg
     5941 tctacagcca gaaccgcgaa gtggcgatcc tggaatacct caccctgtcg agctattact
     6001 actggggctc ctatgacatc gccgaccaga attcatcgac caacgtcacc aagagcattc
     6061 attaccccga ggaaagcatc agcccggcca aggagttcac tgccgccaat cacccatatt
     6121 tcgtccgcca tctggtcggc ctgccatcgc ctactgaaag cttcgtgcgc aattacggcc
     6181 cgcgtctgca ccacattgcg ctgtcggtgg ccgacggcga agtcgagggc aaggccaaca
     6241 tcgattatgt ggtggacgcc atcaaggcca agggcaagga tttcctgctg gacgttatcg
     6301 gctccaaaga ggagggcttg aagcagattt tttccagtgc ctccgagcat tcctctctga
     6361 tcatcgaata cgtccagcgt ttcggcgatt tcgacggctt cttcaccaag cagaacgtag
     6421 ccgaactgac cgaagccgct ggcgtcgagg agaatctgcg attgctgcag gctgaaagca
     6481 cggcatgacc atcaaagttg cgattcacca caaaactgcc taccactacg accatccggt
     6541 ccagctcacc ccgcacgtgt tccggctgcg tccggcgacg cattcgcgca ccccgatctc
     6601 agcctattcg ctgaagatca agccggaaaa gcatttcatc aactggcagc aggatccgtt
     6661 tggcaactat ctggcgcggg tggtgttccc ggagaagtcc agggaactgt cgatcgaggt
     6721 ggatatggtt gccgaaatga cggtgatcaa cccgttcgat tttttcctgg aagagtacgc
     6781 cgagcatttt ccattcagct atccggaaca gctgcaaaaa gaactcacgc cctatctgga
     6841 ggtgcgcgag gatggtccgc tgctgcggca gttcctggcc ggagccgacc acggcaagca
     6901 gggtatcgtc gattttctgg tagggatcaa ccagcgcgtc aaccagactg tcaattattc
     6961 catccgcatg gagcccggcg tgcagacttg tgaggaaacg ctggaaaaga agctcggctc
     7021 ctgccgtgat tccggctggc tgctggtcca ggcgctgcgc caaatgggcc tggccgcacg
     7081 tttcgtgtcg ggttatctgg tgcagctggt ggcagacgtc aaatcgctgg acgggccttc
     7141 cggcaccgac aaggatttca ccgacctgca cgcctgggcc gaagtctaca ttcccggcgc
     7201 tggctggatc ggcctcgacc cgacttccgg gctgttcgcg ggcgagggtc atatcccgct
     7261 cgcctgcacc gccgattacg tcagc
//
LOCUS       DAOMFA010000078         7281 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_1232_length_7281_cov_5.246609, whole genome shotgun sequence.
ACCESSION   DAOMFA010000078 DAOMFA010000000
VERSION     DAOMFA010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 7281)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7281)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7281
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1232_length_7281_cov_5.246609"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..328)
                     /locus_tag="PKW44_05225"
     CDS             complement(<1..328)
                     /locus_tag="PKW44_05225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR51020.1"
                     /translation="MTTKSIMAAAIVVALVSTVASAQMIIYPAKGQSAQQQQKDEAEC
                     YTWAKGKTGYDPVQATQTVAPPPPQQKVGGERLGGAARGALGGAVIGAIAGDTGGGAA
                     IGAAGGT"
     gene            complement(400..939)
                     /locus_tag="PKW44_05230"
     CDS             complement(400..939)
                     /locus_tag="PKW44_05230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR51021.1"
                     /translation="MKKYSLNLFSSIGFVLATLLALPAFAADNAVAGSADKSAASSDS
                     SGSAEKMEILRQKLSADKKLVVANNMNLTDSEAKAFWPVYEAYQQDLYQINLRMAKVI
                     NDYALAYNKGAVLNDTAKKLLDESIAIELAEAKLKQSYVPKLSKVLPAAKVARYIQIE
                     NKIRAIIRYELAEAIPLVD"
     gene            complement(1034..1681)
                     /locus_tag="PKW44_05235"
     CDS             complement(1034..1681)
                     /locus_tag="PKW44_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4197 domain-containing protein"
                     /protein_id="HQR51022.1"
                     /translation="MTVPAFAQGLDDLSNKDAVGGLKAALTQAADTAVAELGVENGFF
                     DNAKVKIPLPDTLKKAEKAMRMFGMGKEADELVLRMNRAAEAAVPEAKALLTDAVKKM
                     SVQDAKDILTGGDDAATQYFRKTTSGPMAQKFLPIVKKATQDVQLAQQYNQFAEAGAR
                     FGLVRKDQASLEQYVTQKTLDGVYTMMAEEEKAIRQDPMGQASSLIKKVFGAVGN"
     gene            complement(1752..2738)
                     /locus_tag="PKW44_05240"
     CDS             complement(1752..2738)
                     /locus_tag="PKW44_05240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR51023.1"
                     /translation="MALLPVSAWAWGHAGGGGFRGGGGGFHGGGFGGASHDDGASHSG
                     SFGGESHSGQTESHSGEYGSTTHSEGSTTHTNNYGDTTTHTQGQGTTATGAYGGTATH
                     QEGSDQTTMSNSYGSATHTRDQGTTATNNYGDTATHNEGSGSTTVTNSYGDSATHYAG
                     GGTYASTPYGTAYHPPTYAYPPPTTVAYYGDGCYDCVSPAGAAAAGAMVGMAAGAAME
                     SSSASAANSNAYSAGYNAGTTNTSAAYSAGYNAGATNTAAASAAGYNAGVTAGQAAPA
                     QPVYTVGMTVASLPAGTIPVETNGTHYFLSGNTWFLPAYGANGVYYRVVPAP"
     gene            complement(2790..3614)
                     /locus_tag="PKW44_05245"
     CDS             complement(2790..3614)
                     /locus_tag="PKW44_05245"
                     /inference="COORDINATES: protein motif:HMM:NF021391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2092 domain-containing protein"
                     /protein_id="HQR51024.1"
                     /translation="MSRTIWALLVALGMMCVGVGALAGQPDAPGSQVASQSVRPALEA
                     RAIDLLKAMSGRLAAARSLSFTAVISYESSSRLGPPLVYTTKSDVLLQRQDKLRVITL
                     GDGPASEFYYDGKTMVAFAPAENLAATAEAPPDIDEALKAAFDSHGTYFPFADVIVAD
                     PYADMADGMQRAFYMGQSRIVGGTTTDMVGVVTNGVFEQIWIGAEDKLPRRLRAVYLD
                     DPAQLRHDMELSNWKINPAVPAGAFASKKAAKAQPMPFVHPDSLPAPTTTTKPEMP"
     gene            complement(3611..3892)
                     /locus_tag="PKW44_05250"
     CDS             complement(3611..3892)
                     /locus_tag="PKW44_05250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR51025.1"
                     /translation="MKPQYLYALLPALLVAGTAQADYPMVNIAADKVIAKYQNATCEQ
                     LWEQKGQPKTDEEQNVIQILHDDPQVRTVFLDKIAAPVVNKLFECGLIP"
     gene            complement(3985..4671)
                     /locus_tag="PKW44_05255"
     CDS             complement(3985..4671)
                     /locus_tag="PKW44_05255"
                     /inference="COORDINATES: protein motif:HMM:NF014025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HQR51026.1"
                     /translation="MNTSSILSTFAATSFALLNPLGMLPIFMGYSASLKGGAQRWLAL
                     LVSLTVMGLTLVFLLTGNAIIDFFGITLDSFRVAGGILLLLIGINLVMGDPAKAAKEM
                     VVEAQSSELGTAKSVYNQIVVPFAMPLLVGPGVIANLILYAGEARTLHNGELFFGLIA
                     ITIGMALVTLIILLSGRALRRVLGDVGLSILTRILGLLVASIGVQFVITGGTDIIVHS
                     IAPAVLGIPR"
     gene            complement(4715..5347)
                     /locus_tag="PKW44_05260"
     CDS             complement(4715..5347)
                     /locus_tag="PKW44_05260"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQR51027.1"
                     /translation="MPLINILVLCFCLALAVQPAFAGFDEAAAAFASGDYDRALKEVR
                     PLAEKGDPRSQYAMGVLYENGFAVAKDLKQAAAWYLKAAKQGNSDAQYNLGAMYEHGV
                     GMPVNYPEAARWYRPAAEQGDIDALSNLGVLYQNGHGVTQNKVLALALYNVSVVYAGT
                     GKTQAAQNRQILANQMPLNDVKKAQSLTEELLKPGNLKPGLEAYLKQPQN"
     gene            complement(5424..>7281)
                     /locus_tag="PKW44_05265"
     CDS             complement(5424..>7281)
                     /locus_tag="PKW44_05265"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HQR51028.1"
                     /translation="ANQWQPNLFRNTTQIYPFAGKPGWNLITGMADDAIDYLNRINQT
                     MPSKPFFVKYAPGATHAPHHPTKEWVDKIHNMHLFDQGWNKLREQIFENQKKLGVIPQ
                     NTQLEPWPTKVLPNWDSLTPEEKKLYIRQVEVFAAYAAYSDHEIGRVVQAIEDMGKLD
                     NTLIIYINGDNGTSAEGGPLGTPNEVAFFNGVSVPVADQMKWYDVWGTEQTYNHMSAG
                     WSWAFDTPFTWFKQNASRLGGIRQNMAVSWSGHIKDQGALREQFMHIIDVVPTILEVA
                     GIKAPMMVDGIKQAPIEGTSFAYTFDAKNAKAPSRHKTQYFEMMGQWALYHEGWLLST
                     QVNRAPWEVMGAANPDPLNNQVLELYNLNTDFSQSQNIAAKHPDKVKQMKQMFIAEAR
                     KYQVFPLDASVAGRIVAPRPNITAGRSEFVYTKPMTGLPQGDSPFLLDSSYTITADIE
                     VPKGGAEGMILTSGGRFAGYGFYLLKGKPVFLWNLIDLKRIKWEGPDALTPGKHLLEF
                     DFKYDGLGVGTLAFNNMSGLGRPGTGTLKVDGKAVQTITMPHTLPMILQWDESFDVGS
                     DTLTGVNDADYQPPFALTAKLNKLTIKVDRPQLSPEDIKKLEAAMRNNKASE"
BASE COUNT         1345 a         2222 c         2109 g         1605 t
ORIGIN      
        1 ttgttccccc cgccgcacca atcgccgctc ccccgcccgt atccccggca atcgccccaa
       61 tcacagcacc tccgagcgcg ccacgggctg cgccgcccaa acgttcgccg ccaactttct
      121 gctgcggtgg cggtggcgcc acggtctggg tcgcctgcac cggatcatag ccagttttcc
      181 ccttggccca ggtgtagcat tcggcctcat ccttctgctg ctgctgggct gactgaccct
      241 tggccgggta aataatcatc tgcgcactcg ccaccgtgct gacgagcgcc acgacgatcg
      301 cagccgccat gattgatttt gtagtcatct ttatcgcctt tcgtacactt caataaattc
      361 tgttgttcca gccaaatcca gcgatgtcaa agctgtggct caatcgacca gcgggatcgc
      421 ctccgcaagt tcgtagcgga tgatggctcg aatcttgttc tcgatctgga tgtagcgcgc
      481 caccttggct gccggcaaca ccttgctcag ctttggcaca tacgactgtt tcagcttagc
      541 ctcagccagt tcgatcgcaa tggattcatc cagcagcttc ttcgccgtgt cgttcagcac
      601 cgcccccttg ttgtaggcca gggcatagtc attgatcacc ttggccatgc gcaggttgat
      661 ctggtacaga tcctgctggt aggcctcgta gaccggccag aatgccttgg cctcactgtc
      721 ggtcaggttc atgttgttcg ccaccaccag cttcttgtct gcgctcagct tctgccgcag
      781 gatttccatc ttttctgccg agccggaact gtcactactg gccgcgcttt tatcagcact
      841 accggccact gcattatcgg cagcaaatgc tggcagggcc agtaacgtag cgaggacaaa
      901 ccctatggaa ctgaacagat taagggaata tttcttcatc attgcttact ccaaaagttg
      961 gttagattcg aatcgcctgc aattgaccgg cctcctccaa gggtggcggc ccgacatgcc
     1021 ttgggcagct ggattaattg ccgaccgcac caaacacctt cttgatcagg ctgctggcct
     1081 gccccatggg atcctgacgg atagcctttt cctcttcggc catcatggtg tatacgccgt
     1141 ccagcgtctt ctgggtgaca tactgctcca ggctggcctg atccttcctg accaggccga
     1201 acctggcgcc ggcttcggcg aactggttgt actgctgggc cagttgcaca tcctgggtgg
     1261 cctttttcac gatgggcagg aacttctgcg ccatcgggcc ggaagtggtt ttcctgaaat
     1321 actgggtcgc cgcgtcatcg ccaccggtca ggatatcctt ggcgtcctgc acgctcattt
     1381 tcttcaccgc atccgtgagc agcgccttgg cttccggcac ggcagcctcg gcggcgcggt
     1441 tcatgcgcag caccagttcg tcggcctcct tgcccatgcc gaacatgcgc atggcctttt
     1501 cggctttctt cagcgtgtcc ggcaacggga tcttgacctt ggcgttgtcg aaaaatccgt
     1561 tttccacgcc aagctcggcc accgcggtat cagcagcctg ggtcaaggcc gccttcaggc
     1621 cgcccacggc gtccttgttg ctcaggtcat ccaagccctg cgcaaaagcg gggaccgtca
     1681 ttactactga tatcagaaat ccaaccatcc aacgcacgtc actctcctaa aaaatgcagg
     1741 gctccgccct gtcacggggc aggcactacg cggtagtaaa ccccgttcgc cccataggcc
     1801 ggcaggaacc aggtattgcc gctcaggaag taatgcgtgc cgtttgtttc gacgggaatc
     1861 gtgccagcgg gcagtgacgc aacggtcatg cctactgtgt agacgggctg cgctggcgcc
     1921 gcctggccgg cagtcacgcc ggcgttgtag cccgccgcgc ttgccgcagc cgtgttggtg
     1981 gcaccagcgt tatagcctgc cgagtaagcg gcggacgtat tggtcgtgcc ggcattgtag
     2041 cctgccgaat aggcattcga gttcgcagcg ctggcgctgg atgactccat ggcagcgccg
     2101 gcggccatgc caaccatcgc accggctgcc gccgcacccg ccggagacac gcaatcgtag
     2161 caaccgtccc cgtagtaagc caccgtggta ggcggagggt atgcgtaagt gggcgggtgg
     2221 taggcagttc cgtagggcgt gctcgcatag gtgccgccac cggcatagtg cgtcgcgctg
     2281 tcgccatagg agttggtaac ggtggtagag cccgatcctt cattgtgcgt agcggtatcg
     2341 ccgtaattgt tggtggcggt cgtgccctga tcccgggtat gcgtcgcgct accgtaggaa
     2401 ttggacatcg tggtctggtc actgccctcc tgatgcgtcg cggtaccgcc ataggcgccg
     2461 gtcgccgtcg taccctgccc ctgggtgtgc gtcgtggtgt cgccgtagtt gttggtgtgg
     2521 gtagtggagc cttcgctgtg cgtcgtgctg ccgtattcgc cgctatggga ttcggtctgg
     2581 ccgctgtgcg actcaccacc aaaactgccg gaatgcgatg ccccatcgtc gtgcgatgcg
     2641 ccgccaaaac ctccaccatg aaacccgcca ccaccgccgc ggaatccgcc gccgcccgca
     2701 tgcccccacg cccatgctga aacaggtaac agcgccatgc acagcaacca aaaaaagcga
     2761 atgagattat ttctgaccat gatgccccct tatggcattt caggcttggt tgtggtggtc
     2821 ggggccggca ggctgtccgg gtggacaaac ggcatgggct gcgccttggc ggcctttttc
     2881 gaggcgaagg cacccgctgg aacagcaggg ttaatcttcc agttggataa ttccatgtca
     2941 tggcgcaatt gcgcaggatc gtcgaggtaa accgcgcgca gcctgcgcgg cagcttgtcc
     3001 tcggcgccta tccagatctg ttcgaagacc ccgttggtga ccacgccgac catatcggtc
     3061 gtcgttcccc cgacaatacg ggattgcccc atgtaaaaag cgcgttgcat gccatcggcc
     3121 atatctgcat agggatcagc tacgatgaca tctgcgaacg ggaaataggt gccatgactg
     3181 tcgaaagccg ccttcagggc ttcgtcaata tcgggagggg cctccgcggt ggcggccagg
     3241 ttctcggccg gcgcgaatgc caccatggtc ttgccgtcat agtagaactc ggaagccggg
     3301 ccatcgccga gagtaatcac ccgcagcttg tcttgtcgct gcagcagcac gtccgacttc
     3361 gtcgtataca cgaggggcgg tcccaaccgg ctcgaactct cgtaactgat gaccgcagtg
     3421 aacgaaagcg atcgagctgc tgccaggcgg ccgctcatgg ccttcagcag atcaatggcg
     3481 cgcgcctcca gtgccggcct gacggattga gacgccacct gacttcccgg agcatctggt
     3541 tgacctgcaa gcgcgccgac acccacgcac atcatcccca gcgccaccaa caacgcccat
     3601 atggtacgac tcatggaatc agcccgcatt cgaacagctt gttgacgacc ggcgccgcga
     3661 tcttgtcaag aaaaaccgtg cgcacctgtg ggtcgtcgtg cagtatctgg atcacattct
     3721 gttcttcatc cgttttgggc tggcctttct gttcccacaa ctgctcgcag gtcgcattct
     3781 ggtacttggc aatcaccttg tcggccgcga tattcaccat cgggtaatcc gcctgggcgg
     3841 ttccggcaac aagcagcgca ggcaacaatg cgtacaagta ttgtggtttc atggttttct
     3901 cctgttgttg agttaaatac aggtacatct ttttcacttg ataattctag tcttggactt
     3961 ctgttcgtgg ccactcgcag gtattcagcg cgggattccc aatacggccg gggcaatcga
     4021 gtgaacgatg atgtcggttc cccccgtaat gacgaactgt accccgatag aggctaccag
     4081 caaaccaagg atgcgggtca ggatactgag gccgacgtcg cccagcacgc gccgcaacgc
     4141 cctgcccgag agcaggataa tcagcgtgac cagggccatg ccgatggtga tggcgatcag
     4201 tccgaaaaag agttccccat tatggagtgt gcgcgcctcg cccgcataca gaatcaggtt
     4261 ggcgatgacg ccggggccca ccagtagcgg catggcaaat gggaccacga tctggttata
     4321 tacggatttt gccgtgccca actcgctcga ctgcgcctcg accaccatct ccttcgccgc
     4381 ctttgcggga tcacccatga caagattgat gccgatcaac agcagcagga tgccgccggc
     4441 cacgcggaag gaatccagcg tgatgccgaa aaaatcgatg atggcattac ccgtcagcag
     4501 aaacaccagg gtcaggccca tgaccgtcaa cgatacgagc agcgcaagcc aacgctgcgc
     4561 tcctcccttc agactagcgc tataacccat gaaaatgggg agcatcccca gcggattcag
     4621 caatgcgaag gaagtggcgg cgaaggtact cagaatgctg cttgtattca ttggatgtcc
     4681 tctagggtgt gtctagacta acttcgcatg ccgttcaatt ttgcggttgt ttcaggtagg
     4741 cctccagccc tggcttaagg ttgccaggct tgagcaattc ttccgtgagc gactgggctt
     4801 tcttcacatc gttgagcggc atctggttag ccaggatctg gcgattctgc gccgcctgag
     4861 tcttgccggt gcccgcatag accaccgaaa cattatatag cgccagggcc agcaccttgt
     4921 tctgcgttac accatggcca ttctggtaga gcacacccag gttgctcagc gcatcgatat
     4981 ccccctgttc cgcggctgga cggtaccagc gagcggcctc ggggtaattc accggcatgc
     5041 ctacgccatg ctcgtacatc gcgccgaggt tgtactgggc gtcgctgttg ccttgtttcg
     5101 ccgccttcag ataccaggcc gccgcctgtt tcaggtcttt tgccaccgcg aagccgtttt
     5161 cgtagagcac gcccatggcg tactggctgc gcggatcgcc cttttcggcc agcgggcgga
     5221 cttccttcag tgccctgtcg tagtcgccgg atgcgaaggc cgcagcggct tcgtcgaagc
     5281 cagcgaacgc cggctgcacg gccagcgcca gacaaaagca gagtaccaga atgttgatca
     5341 acggcatatg aggcttccat tgaacccgaa ttacgggccg ccccgtgagg gacggcccgt
     5401 gtggttaaca acaaaaacga cctctactcg ctggctttgt tgttgcgcat cgcggcctca
     5461 agcttcttga tatcctcggg cgacaactgc ggccggtcga ccttgatcgt cagcttgttg
     5521 agcttggcgg tcagggcgaa cggtggctgg tagtcggcgt cgttcacgcc ggtcagcgtg
     5581 tcggagccaa cgtcgaagct ctcgtcccat tgcaggatca tgggcagggt gtgcggcatg
     5641 gtgatggtct gcacggcctt gccgtccacc ttgagcgtac cggtgccggg acgcccgagg
     5701 ccgctcatgt tgttgaaggc gagcgtgccc acgccgaggc cgtcatactt gaagtcgaac
     5761 tccagcaggt gcttgccagg ggtgagcgcg tccgggcctt cccacttgat gcgcttcagg
     5821 tcgatcaggt tccacaggaa caccggcttg cccttgagca gataaaagcc atagccggca
     5881 aagcgcccgc cggaggtcag gatcatgcct tccgccccgc ctttcggcac ctcaatatcc
     5941 gccgtgatgg tgtaggaact gtcaagcagg aacggcgaat cgccctgcgg cagaccggtc
     6001 atcggcttgg tgtagacgaa ctcgctacgg ccagcggtga tgtttggacg cggggccacg
     6061 atacgcccgg cgaccgaggc atctagcggg aaaacctgat atttcctggc ttccgcgatg
     6121 aacatctgtt tcatctgctt gactttgtca gggtgcttgg ccgcgatatt ctgcgactgg
     6181 ctgaagtccg tgttcaggtt gtagagttcg agcacctgat tgttgagcgg gtcgggattg
     6241 gcggcgccca ttacctccca cggcgcacgg ttgacctggg tactcaacag ccagccttcg
     6301 tgatacaggg cccactgtcc catcatctcg aagtactggg tcttgtgccg cgacggcgcc
     6361 ttggcgtttt tcgcgtcgaa ggtataggcg aaactggtac cttcgatcgg cgcctgcttg
     6421 atgccgtcga ccatcatcgg cgccttgatg ccagcgactt caaggatggt gggcaccacg
     6481 tcgatgatat gcatgaactg ctcgcgcagg gcaccctggt ccttgatgtg gccagaccag
     6541 gataccgcca tgttctggcg gatgccgccg agcctggagg cattttgctt gaaccaggta
     6601 aaaggcgtat cgaaggccca ggaccagccc gccgacatgt ggttgtaggt ctgctcggta
     6661 ccccacacgt cataccattt catctgatcc gcaaccggca cactgacgcc attgaagaac
     6721 gcgacttcgt tgggcgtgcc aagcgggccg ccctcagcgc tggtaccgtt gtcgccgttg
     6781 atgtagatga tgagcgtgtt gtcgagcttg cccatgtctt cgattgcctg cacgacccgc
     6841 ccgatttcgt ggtcgctgta ggcggcatag gcggcgaaga cttccacctg cctgatatag
     6901 agcttctttt cttccggcgt gaggctatcc cagttcggca gcaccttggt cggccaaggc
     6961 tccagttggg tattctgcgg aatcacgccc agtttcttct ggttctcgaa tatctgctcg
     7021 cgcagcttgt tccagccctg gtcgaacagg tgcatgttgt ggatcttgtc cacccactcc
     7081 ttggtcgggt gatgcggcgc gtgcgtggca cccggtgcat atttgacaaa gaacggcttg
     7141 ctgggcatgg tctggttgat gcggttgagg taatcgatag cgtcatcggc catgcccgtg
     7201 atcagattcc agcccggctt gcccgcgaac ggatagatct gcgtggtgtt gcggaacagg
     7261 ttcggctgcc actggttggc g
//
LOCUS       DAOMFA010000079         7271 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_1235_length_7271_cov_5.372367, whole genome shotgun sequence.
ACCESSION   DAOMFA010000079 DAOMFA010000000
VERSION     DAOMFA010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 7271)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7271)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7271
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1235_length_7271_cov_5.372367"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..776)
                     /gene="hemH"
                     /locus_tag="PKW44_05270"
     CDS             complement(<1..776)
                     /gene="hemH"
                     /locus_tag="PKW44_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HQR51029.1"
                     /translation="MACFKPEPPYIHGTSPRIGILLLNLGTPDAPTAPALRRYLKQFL
                     SDRRVVEIPRAVWWLILNGIILNTRPRKSAEKYAQIWTKDGSPLLVHARKQATLLQGF
                     LGVRGVSVEVEFGMRYGNPSTTSALERLKAGNCDRILVIPLYPQYAASSTASALDAVW
                     AALQHTRNVPEIRTVRHYHDHPAYIAALAASVRQHWTVHGRPDKLLISFHGIPRKSLE
                     RGDPYHCECHRTGLLLAEALGLESGQYHICFQSRFGRAEW"
     gene            complement(779..1795)
                     /gene="hrcA"
                     /locus_tag="PKW44_05275"
     CDS             complement(779..1795)
                     /gene="hrcA"
                     /locus_tag="PKW44_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HQR51030.1"
                     /translation="MDKRAQILLKTLVEHYISDGQPVGSRTLSKCSGLDLSPASIRNV
                     MSDLEEMGFIVSPHTSAGRIPTPRGYRLFVDTLLTVQPLHQEEIRQLEHRLSSPDPQE
                     LIASAAGLLSNLTRFAGLVMIPKRRSIAFRQLEFIPLSDKRILLIIVTTDGNVQNRII
                     STERNYSPSELVQAANFFNQNYAGSTFEAVQKKLHEELKQMQTDMTRLMTAALEASGK
                     ALADDHDDVVISGERNLLQVDDLSTNVTSLRKLFDVFERRTTLLQLLDTSHRAEGVQI
                     FIGGESGHLPLDECSLVTAPYEVDGQVVGTLGVIGPTRMAYERVIPIVDITAKLLSNA
                     LSVN"
     gene            complement(1822..3735)
                     /locus_tag="PKW44_05280"
     CDS             complement(1822..3735)
                     /locus_tag="PKW44_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HQR51031.1"
                     /translation="MLNSSDQRRLLHYFFLATWLVLVFVTRDTVNFAAARAHGIPVLA
                     FLLTAWLSYCFIYLLPAMLLAKLADWLVHRKRGPNEAVGRRIVYGVAVLTTGLTTLFL
                     YANTKLYALYGIFVNGFVVNLITTPGGIQSLGGSGSTNLTFAAVALAFVLAQALLLAL
                     VHAIYQKTLHRQIMPKPLFRMLLVVFAVAAVGERAAYAYSEAVAETSVLTLSEGIPFY
                     NGITARSFFKKIGMKVKRQDKFQEVKGRLRYPLQPLNITAPQKPYNIIWLAAESWRAD
                     TLDAEIMPHTWAFAEQANRYTHNYSGGNGTRVGIFTMFTGIPGNYWFPFMDEHQGAAV
                     IDVMQRQNYQMNLFTSAKFSYPEFDRTIFRHIPAAQLHEADESQPGWRNDQINVGKML
                     DFIGQRDPGRPFFSFMFFESPHARYYFPPESVIRRPYRDDINYATLDKDELAKNIIPI
                     KNRYLNAVHHLDSQFGRIFDYLQQNNLLDSTIVILLGDHGEEFMEHGYWGHNSTFVDP
                     QIRTPLVLWIPGKKSGVHDKMTSHMDIIPTIMPLLGVSNPVGDYSTGLNLLSDKQRSS
                     TVLADWSRMAYVDNDVKIVMPIKFNGLMRSRISTASDEDIPPARNGEIFATKNPQLVQ
                     LMQDMGHFMEKKR"
     gene            3862..4725
                     /locus_tag="PKW44_05285"
     CDS             3862..4725
                     /locus_tag="PKW44_05285"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0019674 - NAD metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HQR51032.1"
                     /translation="MKNAFQRVALIGKITNPAIGEHVLALAHFLRERGIAVLVEEKTA
                     AALSIESYATVHIDAIGTHVDLAVVLGGDGTMLAVARGLVEYGVPLVGVNKGRLGFLT
                     DLTTEAIHQAMSDILDGEYTLEQRILLGATIVRDGVAVSAGRALNDVVISKGAMARLI
                     ELELIIDGEFVHRQRSDGLILATPTGTTAYALSAGGPLLHPTLEAIALVPVCPHTLSN
                     RPFAISSASTVEVTLVQADDARVHFDGQLHAELKCGDKVVVHRLQKAVTLLHPRNHSH
                     YAMLRQKLHWG"
     gene            4830..6482
                     /gene="recN"
                     /locus_tag="PKW44_05290"
     CDS             4830..6482
                     /gene="recN"
                     /locus_tag="PKW44_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HQR51033.1"
                     /translation="MLTSLSLRDFVIVDRLELEFAAGFSVLTGETGAGKSILIDALSL
                     ALGERGESGVVRAGCDKADISAEFDIAALPELQAWLVDNALDDGANCLLRRVVHADGR
                     SRAFINGVSATMQQLREAGDFLVDIYSQHAHHSLLKAATQRALLDDFGGLADLAVQVA
                     AAYKRWRELHERRVEMERNAAAYAEELANLRDEVRELAQLNPSAEEWDSLQQEHGRLA
                     HAASLLEGSEQCHELLAEAEVSTLRQLYLVQHKLQELREYDPAMQEALDSLDAAEIQL
                     AEADRFLKKYLSRAELDPERLNEVESRIQAIHAAARKHRVRPEELPELLAKWQQRVQE
                     LESATGDGALARQEEAAREAYQVLAAELGRGRVKAAGELSTKVSAEMQRLALSGGRFE
                     VALQALPEGTAQGLEQVEFLVAGHAGMEPRPLSKVASGGELSRISLAIRVITAQQGET
                     PTMIFDEVDVGIGGGVAEIVGQLLQRLGETRQVLVITHLPQVAAQGAQHYRVSKSTAN
                     GATLSQIEALGQPARTEEIARMLGGVEITETTRKHAAEMLGG"
     gene            6541..7254
                     /locus_tag="PKW44_05295"
     CDS             6541..7254
                     /locus_tag="PKW44_05295"
                     /inference="COORDINATES: protein motif:HMM:NF019500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel"
                     /protein_id="HQR51034.1"
                     /translation="MKTTEQIRLEGTEKDNSTCRHVTCPPRLAQVLENRCFLLLLALI
                     VLLVATPFLAGSVGGKKIIGLLNVIILVTAVVAVERSRLSFAIATFLGLPTLAFQILA
                     LQSGLQGHFALCWGFAAAIYAFTIASLLHYVLRRDIMTADKLYGAAAAYIMIAILWAF
                     LYGVLQYFYPGAYTYQGVPKVLDMTSELIFFSFTVLTTAGFGDITPMAIFSRYLTILE
                     AVTGVMYVGNPPIFNWGQK"
BASE COUNT         1348 a         2102 c         2364 g         1457 t
ORIGIN      
        1 aaccattcgg cgcggccgaa tcgcgactgg aagcagatgt gatactggcc ggattcgagt
       61 cccaaggctt ccgccagcag gaggccggtt ctatggcatt cgcagtgata ggggtcgccc
      121 cgctccagcg acttgcgcgg aatgccgtga aagctgatca gcagcttgtc gggacggcca
      181 tgtaccgtcc agtgctggcg cacgctggcg gccagtgccg cgatgtaggc ggggtggtcg
      241 tggtaatggc gcacggtgcg gatttccggc acgttgcgtg tatgctgcaa tgccgcccac
      301 accgcgtcca gcgccgaggc ggtgctgctg gcagcgtatt gcgggtatag cgggatgacc
      361 aggatgcggt cacagtttcc tgccttgagc cgctccagcg ccgaggtagt ggaagggttg
      421 ccgtagcgca tgccgaattc gacttcgacg gaaactccac gcacgccgag aaagccttgc
      481 agcagggtgg cttgcttgcg cgcgtgcacc agcaggggcg atccatcctt ggtccagatt
      541 tgcgcatatt tttctgccga cttgcgcggg cgggtattga ggatgatgcc attcaggatc
      601 agccaccaga ccgcgcgcgg gatttcgacg acgcgccggt cgctgagaaa ctgcttgaga
      661 tagcggcgca aggccggtgc cgtcggagca tccggggtgc ccaggttgag cagcaggatg
      721 ccgatgcgcg gcgacgtgcc gtggatatag ggcggctcgg gtttgaaaca tgccattttc
      781 agttgacgga caaggcgttg gaaagcagtt tggcggtgat gtccacgatc gggattaccc
      841 gttcgtaggc catgcgggtc gggccgatca cgcctagcgt gccgacgacc tgaccgtcga
      901 cttcataggg cgctgtgacc aggctgcact cgtccagcgg caggtggccg gattcgccgc
      961 caatgaaaat ctgtacgcct tccgcgcgat ggctcgtatc cagcaactgc agcagcgtgg
     1021 tgcggcgctc aaacacgtcg aacagtttgc gcaacgaggt gacgttggtg gagaggtcat
     1081 ccacctgtag caggttgcgt tcgccggaaa tcaccacgtc gtcgtggtcg tccgccagcg
     1141 ccttgccgct ggcttccagc gctgcggtca tcaggcgcgt catgtcggtc tgcatctgtt
     1201 tcagttcttc gtgcagcttc ttctgtaccg cctcgaatgt actgccggca tagttctgat
     1261 tgaagaagtt ggccgcctgc accagttccg acgggctgta gttgcgctcg gtgctgatga
     1321 tgcgattctg cacgttgcca tcggtagtga cgatgatcag caggatgcgc ttgtccgaca
     1381 gtgggatgaa ttcgagctgg cgaaaagcga tactgcggcg cttgggaatc atcaccagtc
     1441 cggcgaaccg ggtcagattg gaaagcaggc cggcagcgct ggcgatcagc tcctgcgggt
     1501 caggcgagga cagtcggtgt tccagttgcc ggatttcctc ctggtgtagc ggctgcacgg
     1561 tgagcagggt gtccacgaac agccggtaac cgcgcggggt ggggatgcgg ccggccgagg
     1621 tgtgtgggct gacgatgaag cccatctctt ccagatcaga catcacgttg cggatgctgg
     1681 ccggcgacaa atcgagtccg gaacacttgg acagcgtgcg cgaacccacc ggctggccgt
     1741 cgctgatgta gtgctcgacc agggttttca gcaggatttg tgcgcgcttg tccatcgcca
     1801 ccattatatt actgttgccg ctcagcgttt cttttccatg aagtgcccca tgtcctgcat
     1861 cagctgcacc agctgcgggt tcttggtggc gaagatttcg ccgtttctcg cgggggggat
     1921 gtcctcgtcg ctggcggtcg agatgcggct gcgcatcagg ccgttgaact tgatcggcat
     1981 gacgatcttg acgtcgttgt ccacgtaggc catgcgtgac caatcagcca ggacggtgga
     2041 actgcgctgc ttgtccgaga gcaggttgag gccggtggag tagtcgccga ccgggttgct
     2101 gacgccgagc agcggcatga tggtagggat gatatccata tggctggtca tcttgtcgtg
     2161 cacgccggac tttttgccgg ggatccacag caccagcggc gtgcggatct gtggatcgac
     2221 gaaggtggag ttgtgtcccc agtagccatg ctccatgaat tcctcgccat gatcgccgag
     2281 caggatgacg atagtgctgt ccagcaggtt gttctgctgc aggtaatcga agatgcggcc
     2341 gaactggctg tccaggtgat gtaccgcgtt gagatagcgg ttcttgatgg gaataatgtt
     2401 cttcgccagt tcgtccttgt ccagcgtggc gtagttgatg tcgtcgcggt aaggacggcg
     2461 gatcacgctt tccggcggga agtagtagcg cgcatgcggc gattcgaaaa acatgaagga
     2521 aaagaacggc cggccggggt cgcgctggcc gatgaagtcg agcattttgc cgacgttgat
     2581 ctggtcgttg cgccatcccg gctgggactc gtcggcctcg tgcagctggg cggcggggat
     2641 gtggcggaaa atggtgcgat cgaactccgg gtaggaaaat ttcgcgctgg tgaacaggtt
     2701 catctggtaa ttctgccgct gcatcacgtc gatgaccgcg gcgccctggt gctcgtccat
     2761 gaacgggaac cagtagttgc cgggaatgcc ggtgaacatg gtgaagatgc cgacgcgggt
     2821 gccgttgccg ccgctgtagt tgtgcgtata gcggttggcc tgctcggcga aggcccaggt
     2881 gtgcggcatg atttccgcgt ccagcgtatc ggcacgccag gattccgccg ccagccagat
     2941 gatgttgtag ggtttttgcg gcgcggtgat gttcagcggc tgcaggggat agcgcaaacg
     3001 gcccttcact tcctggaact tgtcctggcg cttgaccttc atgccaattt tcttgaagaa
     3061 gctgcgcgcg gtaatgccgt tgtagaacgg gatgccttcc gacagggtca gcacgctggt
     3121 ctccgccacc gcttcgctgt aggcgtaggc ggcacgctcg cccacggctg caactgcgaa
     3181 cacgaccagc agcatcctga acaatggctt cggcatgatc tggcgatgca gggtcttttg
     3241 gtagatggca tgcacgagcg ccagcagcaa ggcctgcgcc agcacgaagg cgagggcgac
     3301 cgcagcgaag gtcaggttgg tcgagccgct cccgcccagc gactggatgc cgcccggcgt
     3361 ggtaatcagg ttgaccacga agccgttgac gaaaatgccg tacagcgcat acagcttggt
     3421 gttggcgtac aggaacaggg tggtcaggcc ggtcgtcagc acggcaacgc catagacgat
     3481 acgccggccg acggcctcgt ttggtccgcg cttgcggtgg acgagccagt ccgccagttt
     3541 ggccagcagc atagccggca acaggtaaat gaagcagtag ctcagccagg ccgtcagcag
     3601 gaaagccagc accggaatgc catgcgcacg cgccgcggcg aaattgacgg tgtccctggt
     3661 gacgaacacc agcaccagcc aggtggcgag gaaaaagtaa tgcagcaggc ggcgctggtc
     3721 gctggagttc aacatacgga atggggtgcc tcggtaaagg agtgaattgt aactgttttc
     3781 catgcccgtc tgtgactggt gcgagtgctg attagttctt tctggctgcc gcaacagagt
     3841 gatttatgct ttaattcgcc catgaaaaac gcattccaga gggtcgccct cattggcaag
     3901 atcaccaacc cggccatcgg cgagcatgtg ctcgccttgg cgcacttcct ccgcgagcgc
     3961 ggtatcgccg tgctggtgga ggaaaaaact gccgcagcct tgtccatcga gagttacgcc
     4021 accgtgcaca tcgatgccat cggcacccat gtcgatctgg ccgtggtgct ggggggtgac
     4081 ggcaccatgc tggcggtggc gcgcggattg gtcgagtacg gcgtgccgct ggtcggggtg
     4141 aacaaggggc ggctcggttt tctcaccgat cttacgaccg aggccattca tcaggccatg
     4201 tccgatatcc tcgacggcga atatacgctg gagcagcgca tcctgctcgg cgccaccatc
     4261 gtgcgcgacg gcgtggcggt cagcgccggg cgcgcgctca acgacgtggt tatcagcaag
     4321 ggagccatgg cacggctgat cgagctggag ctcatcatcg acggcgagtt cgtgcatcgc
     4381 cagcgttccg acggcctgat cctcgccacc cccaccggca ctaccgccta tgccctgtct
     4441 gccggcggcc cgctgctgca ccctacgctg gaggccatcg ccctggtgcc ggtctgcccg
     4501 cacacgctgt ccaatcgtcc gttcgccatc agcagcgcca gcacggtgga agtcaccctg
     4561 gtgcaggccg acgatgcccg cgtgcatttc gacggccagc tgcacgccga gctcaagtgc
     4621 ggtgacaagg tggtggtgca tcgtctgcag aaagctgtca ctctgctgca tccgcgcaac
     4681 cacagccatt acgccatgct gcgccagaag ctgcactggg ggtagggcgt tttgacagca
     4741 ttgctcaggt ttgtaggagg ggcgccctcg ccccgattgc atcggcggat ttatcgcgcc
     4801 gagggcggcg ttcctacggt tcgatttgaa tgctgacctc tctctccctg cgtgacttcg
     4861 ttatcgtcga ccggctcgaa ctggagttcg ccgccggttt cagcgtactc accggcgaaa
     4921 ccggcgccgg caaatccatc ctgatcgatg ccctgtcgct agccttgggt gagcgcggcg
     4981 aatccggcgt ggtgcgggcg ggttgcgaca aggccgacat cagcgccgaa ttcgacatcg
     5041 ccgccctgcc ggaattgcag gcttggctgg tcgacaacgc gctggatgac ggcgccaatt
     5101 gccttttgcg tcgcgtcgtc catgccgatg gacgctcgcg cgccttcatc aacggcgtgt
     5161 ctgcgactat gcagcagctg cgggaggccg gcgatttcct ggtcgatatc tatagccagc
     5221 acgcccacca ctccctgctc aaggctgcga cccagcgcgc cttgctggac gacttcggcg
     5281 gccttgccga cctggctgtg caggttgccg ccgcctacaa gcgctggcgc gagctgcacg
     5341 agcgccgcgt ggaaatggaa cgcaacgccg ccgcctatgc tgaggaactg gccaacctgc
     5401 gcgacgaagt gcgcgaactg gcgcagctta acccctcggc ggaagagtgg gacagcttgc
     5461 agcaggaaca tggccgtctc gcccatgccg ccagcctgct ggaaggatcc gagcagtgcc
     5521 acgaactgct ggcggaggct gaagtttcta ctttacgcca gctgtacctg gtgcagcaca
     5581 agttgcagga attgcgcgaa tacgacccgg ccatgcagga agcgctggac agcctggatg
     5641 ccgccgaaat ccagctcgcc gaggctgacc gcttcctgaa gaaatacctc agccgcgccg
     5701 aacttgaccc ggaacggctg aatgaagtcg aaagccgtat ccaggctatc cacgctgctg
     5761 cgcgcaaaca ccgcgtgcgg ccggaagaac tgcccgaact gctggccaaa tggcagcagc
     5821 gcgtgcagga gctggaaagt gccaccggcg acggcgcact ggcgcggcag gaagaagccg
     5881 cgcgcgaggc gtaccaagtc ctggccgccg aactgggccg cggtcgtgtc aaggctgccg
     5941 gagaactaag cacaaaggtc agcgctgaaa tgcaacgcct ggcgctgtcg ggtggccgct
     6001 tcgaggtggc gctgcaggcg ctgccagaag gaacggcgca aggcctggag caggtggaat
     6061 tcctggttgc tggccacgcc ggcatggagc cgcggcctct atcgaaagtc gcctctggtg
     6121 gcgagttgtc gcgcatcagc ctcgctatcc gcgtcattac cgcgcagcag ggcgaaaccc
     6181 cgaccatgat tttcgatgag gtggatgtcg gcatcggtgg cggcgtggcc gagatcgtcg
     6241 gccagttgct gcagcgtctg ggtgaaaccc ggcaggtgct ggtcatcacc cacttgccgc
     6301 aggtggccgc gcagggtgcg caacattacc gtgtcagtaa gagtaccgcc aacggcgcca
     6361 ccctcagcca aatcgaagcg ctgggccaac ccgcgcgtac cgaggaaatc gcccgcatgc
     6421 tgggcggggt ggaaattacc gaaaccacgc gcaaacatgc ggcggagatg ctaggaggat
     6481 gaagttgatg tgatccgctt tgatagggca catgtggatg cacaaaagac ggacgtaaaa
     6541 ttgaaaacca ctgaacagat cagacttgaa ggaacggaaa aggataattc gacatgtcga
     6601 cacgtaacgt gtcccccgcg cttggcgcag gttctcgaaa atcgctgctt tctcctgctc
     6661 ctggcgctga tcgttctgct ggtcgcgacg ccgtttctgg ccggcagcgt cggtggcaaa
     6721 aagatcattg gtctcctcaa cgtcatcatt ctggtgaccg cggtggtggc ggttgagcga
     6781 tcgaggctct cgtttgctat tgcgactttt ctgggattgc cgacgctcgc ttttcagata
     6841 ctggcgttgc agtcaggctt gcagggacac tttgcgttgt gctggggctt tgccgcggcc
     6901 atctacgctt tcaccatcgc cagtctgctg cattacgtgc tgcgtcggga catcatgacc
     6961 gcggacaaac tgtatggcgc tgctgccgca tacatcatga ttgcgatact gtgggcattc
     7021 ctgtacggcg tgctgcaata cttctaccct ggcgcctata cgtaccaagg cgtgccaaaa
     7081 gttctcgaca tgacatcgga actgatcttt ttcagcttca ccgtgctcac gaccgcgggt
     7141 ttcggtgaca ttactcccat ggcgatcttt tcccgctacc tgacaatcct ggaggcagtg
     7201 accggcgtga tgtacgtggg taatcccccc atttttaact ggggtcagaa gtagagtcat
     7261 gcggccatgg c
//
LOCUS       DAOMFA010000080         7196 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_1253_length_7196_cov_5.072119, whole genome shotgun sequence.
ACCESSION   DAOMFA010000080 DAOMFA010000000
VERSION     DAOMFA010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 7196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7196
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1253_length_7196_cov_5.072119"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..181)
                     /locus_tag="PKW44_05300"
     CDS             complement(<1..181)
                     /locus_tag="PKW44_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HQR51035.1"
                     /translation="MQTSDFDFDLPPELIAQFPSAERSASRLLCLDGNSGALRDAMFR
                     ALPSLIQPGDLLVFND"
     gene            263..349
                     /locus_tag="PKW44_05305"
     tRNA            263..349
                     /locus_tag="PKW44_05305"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:297..299,aa:Leu,seq:caa)
     gene            complement(532..933)
                     /locus_tag="PKW44_05310"
     CDS             complement(532..933)
                     /locus_tag="PKW44_05310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR51036.1"
                     /translation="MRNFLILLLGFSCAPLLSAEGNELIIPETPEQMEQTMGDDDPCD
                     NCGVVTDVRHRNREVGPDPSLEKPGTLTIAEVTPDGVALEHIATHEESTGPWLVTVRY
                     GDEFIVHEQNMRPAVEIGDRVQLVEGKIVPR"
     gene            complement(1001..1675)
                     /locus_tag="PKW44_05315"
     CDS             complement(1001..1675)
                     /locus_tag="PKW44_05315"
                     /inference="COORDINATES: protein motif:HMM:NF023197.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HQR51037.1"
                     /translation="MKNRLRTSLAIFLVVAASAWGQENSSGSPPHSGFLSDYSKLEPA
                     ADREGVWLYLNKTADYSEYKKIMFDPIEVYLVPNPDYNGVQPGVLKRMTDGFYGSFLG
                     ALTPDYQIVHEEGPDVLRVRAAITGVQLAKSPLKPSDFIPIKAVYNLGRDVAGDAPMV
                     AEMTAEMEVLDPDGNRLATAIANRKGNKTLKQGDQVTWEDLQSITDYWAAGFRQRLDQ
                     LRDVSQ"
     gene            1939..2595
                     /locus_tag="PKW44_05320"
     CDS             1939..2595
                     /locus_tag="PKW44_05320"
                     /EC_number="2.1.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008119 - thiopurine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiopurine S-methyltransferase"
                     /protein_id="HQR51038.1"
                     /translation="MLHDFWHDRWQNNQIGFHQGEANAWLQQFWPALAVAPGSRVFVP
                     LCGKSSDMLWLRAQSYAIVGVEISRLAVEAFFAENGMPVTVSTQGEFSVYESDGIRLY
                     CGDYFALTAQHLDGVCAVFDRASLIALPPEMRAGYARHMRQLLPPGSQTLLVAFEYPQ
                     AEMQGPPFSVQEEEVRALYAAHCDITLLHVADILAQEPRFRDKGLSRLQEKVYALTYN
                     "
     gene            2767..3186
                     /locus_tag="PKW44_05325"
     CDS             2767..3186
                     /locus_tag="PKW44_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HQR51039.1"
                     /translation="MHQLTHTLATQTRGRRIYDITDEVRRWAESNSMATGLLTLYIQH
                     TSASLLINENYDPDVVADLERFFARLVPDGDRLFVHTVEGPDDMPAHVRTALTQTHLS
                     IPLVGGQLALGTWQGIFLYEHRHEPATRRVVLHLMGE"
     gene            complement(3236..5521)
                     /gene="clpA"
                     /locus_tag="PKW44_05330"
     CDS             complement(3236..5521)
                     /gene="clpA"
                     /locus_tag="PKW44_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013149031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HQR51040.1"
                     /translation="MIAQELEVSLHMAFMDARQKRHELITVEHLLLAMIDNPTAAQVL
                     RSCGANLDALRRELTGYIEEHTPTVTGSDDVDTQPTLGFQRVIQRAILHVQSSGKNEV
                     TGANVLVAIFSEKDSHAVFFLHQQGVTRLDVVNFISHGIAKVTEDNAKRTETEQETDG
                     EAATPGPLESFTQNLNALVAAGKIDPLIGRAPELERVVQTLCRRRKNNPLLVGEAGVG
                     KTAIAEGLARRIVEKNVPEILADATIYALDMGALLAGTKYRGDFEQRLKAVLKKLSDN
                     PAAILFIDEIHTLIGAGAASGGTLDASNLLKPALSNGSLKCIGATTYQEYRGVFEKDH
                     ALSRRFQKVDVVEPSIAETVEILKGLKSRFEAHHSVKYTASALNTAAELSAKYINDRH
                     LPDKAIDVIDEAGAAQRILPKSRQKKVIGNKEIEDIIAKIARIPPKNISSDDRNALKT
                     LDRDLKAVVFGQDKAIEVLAAAIKMARSGLGNTQKPIGAFLFSGPTGVGKTEVARQLA
                     YTLGIELVRFDMSEYMERHAVSRLIGAPPGYVGFEQGGLLTEAITKHPYCVLLLDEIE
                     KAHPDIFNILLQVMDHGTLTDNNGRKADFRNVTIIMTTNAGAEAISKGGIGFTKAASG
                     GDELAEIKRLFTPEFRNRLDATVSFSPLDHAVIMRVVEKFLMQLEDQLHEKKVEVAFT
                     DKLKEYLAKTGFDPLMGARPMARLIQDTIRRALADELLFGRLANGGLVTVDVDEHDKI
                     ILQFPEIAERASAELAVSHNA"
     gene            complement(5533..5844)
                     /gene="clpS"
                     /locus_tag="PKW44_05335"
     CDS             complement(5533..5844)
                     /gene="clpS"
                     /locus_tag="PKW44_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HQR51041.1"
                     /translation="MARTKHQEGALAEPTTTRQKPPPMYKVLLLNDDFTPMDFVVQVL
                     QQFFAKNREQATQIMLKVHNEGAGVCGIYPHDIAETKVSRVTVFAREHQHPLQCVMEE
                     A"
     gene            complement(5905..7164)
                     /locus_tag="PKW44_05340"
     CDS             complement(5905..7164)
                     /locus_tag="PKW44_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HQR51042.1"
                     /translation="MDDIPLSWQFGALAALLLIAAFFSIAETSLMTLNRYRLRHLANS
                     GHHGAKLATALLAKTDKLLGVILLGNNFANAGSATLVAIITVRLFGEEEWTLAAGTLA
                     VTFAILVFAEISPKVIAATHPEKLAFACSYLLYPLLKLAYPAVWFVNLFVSGFLRLLR
                     IKPNFGESTQAISMEELRTIVTEAGLYIPQKHKSILLNLFDLEKITVDDVMTAHTQIE
                     AVDFDAPIEAIVEQIATSHHTRMLVRQGANEEVIGILHIRKVMYQAQNGDLTQDMLRE
                     VLMEPYYVPSGTPLYTQLQQFQEKRERLALVVDEYGELKGLVTLEDILEEIIGDFTTQ
                     SPSRSAGYHQQADGSWLVDGSAQLRDINRKLGFSFPLDGPKTMNGLVLEHFEDIPEPG
                     TSFKIGHHAVEILQTHERIVKSVRIVL"
BASE COUNT         1443 a         2100 c         2087 g         1566 t
ORIGIN      
        1 tatcgttgaa caccagcagg tcaccaggct ggatcaggga gggcagggcg cggaacatcg
       61 catcgcgcag cgcgccgctg ttgccatcca ggcacagcag ccggctggcg ctgcgctcgg
      121 ccgaggggaa ctgggcaatc agctccggcg gcaggtcgaa atcgaagtcg cttgtttgca
      181 taaaatctgg ctgactaaaa acgaaatgcc cgaatgctat agaaaaccgc gcagattcga
      241 tataatgccg gctcctttcc cagccgggat ggcggaatcg gtagacgcag cggattcaaa
      301 atccgccgct ggtaacagtg tgggggttcg agtccccctc ccggcaccac ccatgtaagt
      361 ctctgctttt tgttagcaaa cgcgccaaat tgcataaggc caagacaacc tctcatactc
      421 ggagccaaca ggggtagttg gccaagtaca gactctaata gctactgaag cagcaattct
      481 tccagaagat tgacgaaacc ctacagaaat catgcctcta gtcgggggcg gctatcgtgg
      541 aacgattttt ccctccacta attgcacacg atccccaatc tctacggcag gtctcatgtt
      601 ctgctcatgg actataaatt catcaccata gcgcactgtc accaaccacg gacccgtaga
      661 ctcttcgtgt gttgcaatat gctccagagc cacgccatcc ggggtcacct ccgcgatagt
      721 taaagtgcct ggcttctcca gcgaaggatc cgggccgact tcccgattcc tatgccggac
      781 gtcggtcacc acaccacagt tatcgcaagg gtcgtcatcc cccatggttt gctccatttg
      841 ctccggggtt tcggggatga tcagttcgtt tccctcggcc gaaagtagcg gagcgcagga
      901 aaaaccaagt aataaaatta gaaagtttct cattcaaaac cctcaactat attaagtgcc
      961 aggaaagcca ctcggccgct ggtcatcagc cgtttataac tcactgagac acgtcccgca
     1021 actggtccag ccgttgccgg aatccggcgg cccagtaatc cgtgatcgat tgcaagtctt
     1081 cccatgtgac ctgatcgccc tgcttcagcg ttttgtttcc cttgcgattc gctatggcgg
     1141 tagcgaggcg gttgccatcc gggtcaagga cctccatctc cgccgtcatt tccgcgacca
     1201 tcggcgcatc accggccacg tcccgtccaa ggttgtacac agccttgatc gggatgaaat
     1261 cggatggttt gagcggcgac ttggccagct gcacaccggt aatcgcggcc ctgacccgca
     1321 gcacgtccgg gccttcctcg tggacgatct ggtaatccgg cgtgagcgcc ccgaggaacg
     1381 aaccgtaaaa gccgtctgtc atgcgcttca acacccccgg ctgcaccccg ttatagtcgg
     1441 gattcggcac cagataaacc tctatcgggt caaacatgat tttcttgtat tcactatagt
     1501 ctgcggtctt gttgagatat agccataccc cctcacggtc ggcggctggt tccagcttgg
     1561 aataatccga gagaaagcca gaatgcggcg ggctgcctga ggagttctcc tggccccaag
     1621 cggatgccgc gactacaaga aaaattgcca gcgacgttct taacctgttt ttcatatagc
     1681 ttctcttttt gacgccccta gagtgatgcc atctgatcta tttcaaggca ctcacctgct
     1741 gcttgcgttc atgcaagacg gtgccaccga cacacagggt tgaacactat cagccgcgac
     1801 ctttcaatca actggattcg gcccggaaat atcatatcgg caccgtagat ccattgtaac
     1861 tggaaacacg aggatagccc cgcatccaca ccgctttccc ggcatagtat tattcagcag
     1921 acacttagga gaaaatccat gctacatgac ttttggcatg atcgctggca aaacaaccag
     1981 atcgggtttc atcagggcga ggcgaatgcc tggttgcaac agttctggcc ggcgttggcc
     2041 gtggctccgg gcagccgggt gtttgtgccc ttgtgcggga aaagtagcga catgctgtgg
     2101 ctgcgcgcgc agagctacgc cattgtcggc gtggaaatca gccggttggc ggtagaggca
     2161 ttttttgccg agaacgggat gccggtgacg gtttcgaccc aaggcgagtt tagcgtctac
     2221 gaaagcgatg gcatccggct ctactgcggc gactatttcg cactgaccgc acaacatctg
     2281 gacggggtat gcgccgtgtt cgaccgcgca tctctcatcg ccctgccgcc ggaaatgcga
     2341 gcgggttatg cccgccacat gaggcagctg ctcccgccgg gcagccagac cttgctggtg
     2401 gcgttcgagt atccgcaagc ggagatgcag ggcccgccat tcagcgtgca ggaagaggag
     2461 gtgcgggcgc tttacgccgc gcattgcgac atcacattgc tgcacgtggc agatatcctc
     2521 gcgcaggaac cccgcttccg cgacaagggc ttaagccgtc tgcaggaaaa ggtttacgcg
     2581 ctgacgtaca attaaactcg gatagttcat tagcccgtca ttcccgcgca ggcgggaatc
     2641 cagtcgattg aaacccccga gtagcgagac aaaattgtgg tcttgtccgc tacgcggagt
     2701 atttttttgc tggatttccg cctgcgcggg aatgacgcgg ggttacctca acaaacagag
     2761 attgccatgc accaactcac ccacaccctg gccacccaaa cccgtggccg ccgcatatac
     2821 gacatcaccg acgaggttcg gcgctgggcg gaaagcaaca gcatggcgac tggcctgctc
     2881 acgttgtaca tccagcacac ttcggccagc ctgctgatca acgaaaacta cgaccctgat
     2941 gtggtcgccg atctggaacg cttcttcgcc cgattggtgc cggacggcga ccgtctgttc
     3001 gtgcataccg ttgaaggccc ggacgacatg ccggcgcatg tgcggacggc actgacccag
     3061 acccatctct caattccact ggtcggcggt cagctcgcgc tgggcacatg gcagggaatc
     3121 ttcctgtacg aacaccgcca cgagccagcc acgcgccgag tggtgctgca tttgatgggg
     3181 gaatgaggct gacgagcgcg ggcgggatcg cttggactgc gcggtgttgc gacgatcagg
     3241 cgttatggct gacagccagt tccgcggaag ccctctccgc aatttccgga aattgcagga
     3301 tgatcttgtc gtgttcgtcc acatctaccg tcaccaagcc gccgttggcc agccggccaa
     3361 acagcagctc gtcggccagt gcacggcgga tggtgtcctg aatcaggcgc gccatgggac
     3421 gggcgcccat caatgggtcg aaaccggttt tggcgagata ttccttgagc ttgtcggtaa
     3481 aggctacttc caccttcttc tcatgcagtt gatcttccag ctgcatgagg aatttttcca
     3541 ccacgcgcat gatgaccgcg tgatcgagcg ggctgaagga aaccgtggca tccaggcggt
     3601 tgcggaactc tggggtgaac agccgcttga tctcggccag ttcatcgcca ccgctggccg
     3661 ccttggtgaa accgattcca cccttggaaa tagcctccgc gccggcattg gtggtcatga
     3721 tgatggtgac gttgcggaaa tccgccttgc gcccgttatt gtcggtcagc gtgccgtgat
     3781 ccatcacctg tagcaggatg ttaaaaatgt ccgggtgcgc cttctcgatc tcatcaagca
     3841 acagcacgca ataagggtgc ttggtgatgg cctccgtcag caggccgccc tgctcgaagc
     3901 cgacatagcc gggcggcgcg ccgatcaggc gtgacaccgc atggcgttcc atgtattccg
     3961 acatgtcgaa gcgtaccagt tcgatgccca gcgtgtaggc cagttgccgt gcaacttctg
     4021 tcttgcccac gccggtaggg ccggagaata agaatgcgcc gatcggtttc tgcgtgttgc
     4081 cgagcccact gcgcgccatc ttgatggcgg cggccaatac ttcgatggct ttgtcctggc
     4141 caaacaccac ggctttgagg tcgcgatcca gcgttttcag cgcattgcga tcgtcgctgg
     4201 aaatattctt gggcgggatg cgcgcgatct tggcgatgat gtcctcaatc tccttgttgc
     4261 cgatcacttt cttctgccgc gattttggca gaatgcgctg cgcggcaccg gcctcgtcga
     4321 tgacgtcgat ggccttgtcc ggcaggtggc ggtcgttgat gtatttggcc gacagctccg
     4381 ccgccgtatt cagcgcggaa gcagtgtact tgacgctgtg gtgcgcctcg aagcgcgatt
     4441 tcagcccctt gaggatttcc accgtctcgg cgatgctggg ttcgaccacg tccactttct
     4501 ggaaacggcg tgacagcgca tggtcctttt cgaagacgcc acgatattcc tgataggtag
     4561 tggcgccgat gcacttgagc gagccgttgg aaagcgccgg cttgagcagg ttggaggcat
     4621 ccagcgtacc gccactggcg gcaccggcgc caatcagggt atggatttca tcgatgaaca
     4681 ggatggcggc agggttgtcg gacagcttct tcagcactgc cttcaggcgc tgctcgaagt
     4741 caccgcggta tttggtgccc gccagcaagg cgcccatatc caaggcatag atggtggcat
     4801 cggctaggat ctccggcaca ttcttttcca cgatgcggcg agccaaacct tcggcaatgg
     4861 cggtcttacc gacgccggct tcgcccacca gcagcgggtt gttcttgcgg cggcggcaca
     4921 gagtctgcac cacgcgctcc agctccggtg cgcgtccgat cagcgggtcg atcttgccgg
     4981 cggctaccag cgcgttcaga ttctgggtga agctctccag cggaccgggt gtcgcggctt
     5041 cgccatccgt ttcctgctct gtctcggtgc gtttggcgtt gtcttcggtg accttggcga
     5101 taccgtgcga aatgaagttg accacatcca gccgggtcac gccctgctgg tggaggaaga
     5161 acacggcgtg tgaatctttc tcgctaaaaa tggcaaccag cacattggca ccggtgactt
     5221 cgttcttgcc ggaggactgc acatgcagga tggcccgctg gatcacgcgc tgaaaaccga
     5281 gcgtgggctg ggtgtcgacg tcatcgctgc cggtaacggt cggcgtgtgt tcctcgatgt
     5341 agccggtcaa ttcacgccgc agggcatcca gattggcacc gcaagagcgc agcacctgcg
     5401 ctgcggtggg gttgtcgatc atcgccagca gcaggtgctc caccgtaatc agctcgtgac
     5461 gcttctggcg cgcatccatg aacgccatgt gcagggaaac ctctaactct tgcgcaatca
     5521 tgttggcacc tttcaagctt cttccatcac acactgcagc ggatgctgat gttcccgggc
     5581 aaatacggtc acccggctga ccttggtttc ggcaatatcg tgcgggtaaa taccacacac
     5641 ccccgcaccc tcattatgca ctttgagcat gatttgcgtc gcctgttcgc gattcttcgc
     5701 aaaaaactgc tgcaagacct gtaccacgaa atccatcggc gtgaagtcgt cgttcagcag
     5761 cagcaccttg tacatcggtg gcggcttttg ccgggtggtg gtcggttcgg caagagcgcc
     5821 ttcttggtgt ttcgtcctag ccatgcttgt agtctatttt gaccctaatc atggattttt
     5881 caagggccat gaggattggg ggtatcaaag aacaatgcgc acgctcttca cgatacgttc
     5941 atgggtttgc aggatctcca ccgcatgatg accaatcttg aaactggtgc cgggctccgg
     6001 gatgtcctcg aaatgctcca gtaccagacc gttcatggtt ttcggcccgt ccagcgggaa
     6061 gctgaagccc agcttgcggt tgatgtcgcg cagctgcgcg ctgccgtcca ccagccagct
     6121 gccgtcggct tgctggtggt agccggcact gcgcgatgga gactgcgtgg tgaaatcacc
     6181 gatgatttct tccagaatgt cttccagcgt caccagcccc ttcagctcgc cgtattcgtc
     6241 caccaccagc gccagccgtt cgcgtttttc ctgaaattgc tgcaattggg tgtaaagcgg
     6301 tgtgccggag ggcacgtagt aaggttccat gagaacttcg cgcaacatgt cctgggtcag
     6361 atctccattc tgcgcctgat acatcacctt gcggatgtgc aggatgccga tcacttcctc
     6421 gttggcgccc tgccgcacca gcatgcgcgt gtggtggctg gtagcgatct gttccacgat
     6481 agcctcgatc ggcgcatcga aatcgactgc ctcgatctgg gtatgggccg tcatgacatc
     6541 gtccacggtg attttttcca ggtcgaacag gttgagcagg atgctcttgt gcttttgcgg
     6601 gatgtaaaga ccggcttccg tgacgatggt gcgcaactcc tccatgctga tggcctgcgt
     6661 gctttcgccg aagttcggct tgatgcgcaa caggcgcagg aaaccgctga cgaacaggtt
     6721 gacgaaccat acggcggggt aagccagctt caacaagggg tacagcaggt agctgcacgc
     6781 gaaagccagc ttttccgggt gggtggcggc gatcaccttg ggcgagattt cggcgaacac
     6841 caggatggca aaggtcaccg ccagcgtgcc cgcggccagc gtccattctt cctcgccgaa
     6901 caggcgcacg gtgatgatgg cgaccagggt tgccgagccg gcattagcga agttgttgcc
     6961 gagcaggatg acgccgagca gcttgtcggt cttggccagc agcgcggtgg ccagtttcgc
     7021 tccatggtgc ccgctgttgg ccagatggcg caggcggtag cggttgagcg tcatcaggct
     7081 ggtttccgcg atcgagaaga aagcggcaat cagcagtagc gcggcaagcg cgccgaactg
     7141 ccaggataag ggaatatcgt ccaagaggtg cggggaaaac cgataagcga agggga
//
LOCUS       DAOMFA010000081         7132 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_1275_length_7132_cov_4.970044, whole genome shotgun sequence.
ACCESSION   DAOMFA010000081 DAOMFA010000000
VERSION     DAOMFA010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 7132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7132
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1275_length_7132_cov_4.970044"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1757)
                     /gene="ppk1"
                     /locus_tag="PKW44_05345"
     CDS             complement(<1..1757)
                     /gene="ppk1"
                     /locus_tag="PKW44_05345"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HQR51043.1"
                     /translation="MATFKLTPDHFLNRELGILEFNRRVLAQAEDERNPLLERLKFLS
                     IVSSNMDEFFEVRVAGLKEQIQYNQSSTAPDGLTPHQIMGRVSAEAHQLIARQYQILN
                     DVVLPRLAEQDIRFLRRSQWNEAQKEWIRSYFFQQLMPILTPIGLDPSHPFPRLLNKS
                     LNFIVELEGKDAFGRKSGNAIVQAPRSLPRVIQLPAEVAGGKYAFVFLSSILHAHVDE
                     LFSGMTVKGCYQFRVTRNSDLSVDDEDTQDLRQALQGELPQRQYGDEVRLEIADTCPA
                     AMSEFLLAKFGLDQDDLYQVHGPVNLIRLMQVPDWVDRPDLKFPPFTPAIPRETSKYK
                     DIFKAIRKSDILLHHPFQSFNPVVEFIQQATDDPQVLAIKMTVYRTSADSTLMKSLIE
                     AAQRGKEVTVVVELMARFDEEANINWAAKLENAGAHVVYGVVGHKTHAKMAMVVRRED
                     DKLRRYVHLATGNYHQRTARLYTDFGLLTCQEDMCSDVNDLFAQLTGLGKASKLKYLW
                     QSPFTLHQKLVKAILNEAEIAKSGKKAHIIAKMNALLDPDIIEALYQASMAGVRIDLI
                     VRGVCALRPGMPGISENIRV"
     gene            complement(1813..2082)
                     /locus_tag="PKW44_05350"
     CDS             complement(1813..2082)
                     /locus_tag="PKW44_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HQR51044.1"
                     /translation="MARTVNCVKLGREAEGMDFPPYPGALGKRIFENVSKEGWAGWLK
                     HQTMLVNENRLSLADPHARKYLMEQTEAYFFGSGAEQAEGYIPPR"
     gene            complement(2099..2371)
                     /locus_tag="PKW44_05355"
     CDS             complement(2099..2371)
                     /locus_tag="PKW44_05355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR51045.1"
                     /translation="MERKRSITLHFTDGTKITFDFPEQTTNVAGKNLRIEDVLTGDHV
                     LIEADGSFLVFPISNIKYVQFSINEAFDLDVVKLPRSVIRNATIRK"
     gene            complement(2402..2845)
                     /locus_tag="PKW44_05360"
     CDS             complement(2402..2845)
                     /locus_tag="PKW44_05360"
                     /inference="COORDINATES: protein motif:HMM:NF017269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HQR51046.1"
                     /translation="MRQHYRSLLLAVSFLAMLAGCASPGGSPSQPAEIRQGKIEQITE
                     IEINENNHPGLGAIIGGIAGAGIGSLIGQGTGKDVAIAVGAIGGAVGGNYLQNKHDKQ
                     PGQQIVVRLRSGVLVVVSQPTNANLYVGQPVYVEGAGMNATVVPQ"
     gene            complement(2897..3184)
                     /locus_tag="PKW44_05365"
     CDS             complement(2897..3184)
                     /locus_tag="PKW44_05365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR51047.1"
                     /translation="MADADQDAGVIQALLDRFNEQKLPRALELKERLQRGEKLNELDL
                     HFLDETFSGFIGMRTLYERHPEFQPLVAKIIGMYNEITRLALENEQKAENS"
     gene            complement(3282..3959)
                     /gene="queC"
                     /locus_tag="PKW44_05370"
     CDS             complement(3282..3959)
                     /gene="queC"
                     /locus_tag="PKW44_05370"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HQR51048.1"
                     /translation="MKKAVVLLSGGLDSATALAIARNEGYACYCLSFDYHQRHVAELQ
                     AAERVTQALGAVEHRVFHLDLAAFGGSALTDARIAVPQAPTEGIPVTYVPARNTIMLS
                     LALAWAEVLGALDIFIGVNALDYSGYPDCRREYIDAFERMANLATRAAVEGSKLRLHT
                     PLIDLTKAEIIQRGYALGVDYAMTVSCYQADNEGRACGVCDSCRFRRTGFQQAGVPDP
                     TAYRRLN"
     gene            complement(3956..4594)
                     /gene="queE"
                     /locus_tag="PKW44_05375"
     CDS             complement(3956..4594)
                     /gene="queE"
                     /locus_tag="PKW44_05375"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019897165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HQR51049.1"
                     /translation="MSLRIHEIFFSLQGEASRVGLPTVFVRLTGCPLRCGYCDTAYAF
                     QGGESLELDEILKRVASHGARHITVTGGEPLAQKDCLPLLNRLCDAGYDVSLETGGSL
                     DIAGVDPRVSVILDIKTPGSDEAGKNLWKNLDLLKPKDEVKFVLCSREDYDWARRVLA
                     DRAIVAKCPVLFSPVYGQLKGRELAEWILADHLPVRFQLQLHKLLWGEEPGR"
     gene            complement(4611..5459)
                     /gene="ybgF"
                     /locus_tag="PKW44_05380"
     CDS             complement(4611..5459)
                     /gene="ybgF"
                     /locus_tag="PKW44_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HQR51050.1"
                     /translation="MKPLVLAFCLLLGGIHAAQAAFFSDDEARKKITELQQQVSQLQG
                     KLDQEAKQRQATDERVVALENQLKNQGMDLLNQIERLNAEISKLKGQMEVTAHDVEVT
                     QKRQRDLYTDTDGRLRKLESGAATATEAAAGAPAQAAAQAATSATMASNTENEQKEYD
                     AAYALTKAGKHAEAVAAFQQFTEHYPNGKLVPNAYYWTGTSQFSLRDYKSAIATQQSL
                     VKQYPDHEKAPDALLNIANSQIQLADVDGARQTLKTLIAKYPQSRAAALGKKRLSAIE
                     SLKSKN"
     gene            complement(5466..6020)
                     /gene="pal"
                     /locus_tag="PKW44_05385"
     CDS             complement(5466..6020)
                     /gene="pal"
                     /locus_tag="PKW44_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HQR51051.1"
                     /translation="MNKQIIGAIVLALFLAACSSKQVKPEPAAPVENKDTTQSAPKPA
                     GKSTDDSAQTKPVDQSKLAVNPLTDPANILSKRSIFFDFDKDEVKPEFRPLVEAHAKY
                     LTSHPNAKVVLQGNADDRGTREYNLALGQRRAASVKKALNLLNASDKQIETVSYGEEK
                     PRCTEQTETCWSQNRRVDIVYEGE"
     gene            complement(6054..7112)
                     /gene="tolB"
                     /locus_tag="PKW44_05390"
     CDS             complement(6054..7112)
                     /gene="tolB"
                     /locus_tag="PKW44_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020182994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HQR51052.1"
                     /translation="MTQRASPAPRRRATPHRHPGLDAGIGRQRAPGRRLAFRTDRRHS
                     LRPGCPPRQLAGLEYDVTPAQLRQTAHKIADIIYEKLTGEPGVFATRITYVTKNGKRF
                     ALVVADADGYNPQTVLASNEPIISPAWSPDGTKLAYVSFEKKKPVIFVQSLVSGQRTV
                     LANYKGNNSAPAWSPDGRRLAIVLTHGENSQVYLINADGTGLQQLSGNRAIETEPDWS
                     PDGKWLYFTSDRGGGPQIYRMSPLGGDAQRVTFEGSYNASPHLSPDGKQLAYIRRDGG
                     KFQVAVQDLASGQVQVLSDSAQDESPSFAPNGRLIIFATSIRGKGVLSVVSADGRVKQ
                     RLTEAADVREPSWSPAAK"
BASE COUNT         1293 a         2069 c         2176 g         1594 t
ORIGIN      
        1 acccggatgt tttccgaaat gccgggcatc ccgggccgta gcgcgcatac gccacgcacg
       61 atgagatcga tcctcacccc tgccatggag gcttgataca gcgcttcgat gatgtccggg
      121 tcgagcaggg cgttcatctt ggcgatgatg tgcgccttct tgccggattt ggcgatttcc
      181 gcttcgttca ggatggcctt gaccagcttc tggtgcaggg tgaacggcga ttgccacagg
      241 tacttgagct tgctggcctt gcccagcccg gtgagttggg cgaacaggtc gttgacatcc
      301 gagcacatgt cctcctgaca ggtcagcaag ccgaaatcgg tatacaggcg cgcggtgcgc
      361 tgatggtagt tgccggtggc aaggtgcaca tagcggcgca gtttatcgtc ttctcttcgc
      421 accaccatgg ccatttttgc atgggtcttg tggccgacca cgccataaac gacatgggct
      481 ccggcgttct ccagcttggc tgcccagttg atgttggctt cttcgtcgaa gcgtgccatc
      541 agttccacca ccaccgtgac ttccttgccg cgctgggccg cctcgatcag agacttcatc
      601 agtgtcgaat cggcgctggt gcggtacacg gtcatcttga tcgccagcac ttgcgggtcg
      661 tcggtcgcct gctgaatgaa ctccactacc ggattgaatg actggaacgg atggtgcagc
      721 aggatatcgc tcttgcggat cgccttgaaa atatccttgt atttgctggt ctctctggga
      781 atcgccgggg tgaacggcgg gaacttcaag tccgggcgat cgacccagtc cggcacctgc
      841 attaggcgta tcaggttgac cgggccgtgc acctgataga gatcgtcctg atccagcccg
      901 aatttggcaa gcaagaactc ggacatggcg gccgggcagg tatcggcgat ctccagccgc
      961 acttcgtcgc cgtattgccg ttgtggaagc tcgccctgca gcgcctggcg caaatcctgg
     1021 gtgtcctcgt catcgacgga aaggtcgctg ttgcgtgtca cgcggaactg gtagcaaccc
     1081 ttcaccgtca tgccggaaaa caattcgtcc acgtgggcat gcaggatgga ggacaggaac
     1141 acgaaagcat atttgccacc ggctacctcc gccggtagct ggatcactcg cggcagcgag
     1201 cgcggcgcct gcacgatggc gttgccggat ttacgaccga aggcgtcctt gccctccagt
     1261 tccacgatga aattcaggct cttgttgagc aggcgcggga acggatgaga gggatccagg
     1321 ccgatagggg tgaggatggg catcagttgc tggaagaagt aggagcgtat ccactccttt
     1381 tgcgcctcgt tccactggct gcgacgcagg aagcggatgt cctgttccgc cagccgaggc
     1441 agcacgacgt cattcaggat ctgatactgg cgggcgatca actggtgtgc ctcggcgctg
     1501 acgcgcccca tgatctggtg cggcgtcagg ccgtccggcg cggtgctgga ctggttatac
     1561 tggatctgct ccttgaggcc ggccacccgc acttcgaaga actcgtccat gttgctgctg
     1621 acgatgctga gaaatttgag ccgttccagc aacgggttgc gttcgtcctc cgcctgcgcc
     1681 agtacgcggc ggttgaactc gagaataccg agttcgcggt tgagaaaatg atctggtgtc
     1741 aatttgaagg tggccaagtc aaaactccca gactagcctg gcagggtgac agtttgatgc
     1801 cgcaatgggt tattacctgg gtgggatgta gccttcggcc tgttccgcgc cgctgccgaa
     1861 aaagtaggcc tcggtctgct ccatcaggta tttgcgtgcg tgtgggtcgg ccagactcag
     1921 gcggttctcg ttcaccagca tggtctggtg cttgagccag ccggcccagc cttccttgga
     1981 cacgttttca aaaatacgct tgcccagcgc accaggatag ggaggaaaat ccatcccttc
     2041 tgcttcgcgt ccgagtttta cgcagtttac tgttcttgcc atgtaagttc ctctgatcct
     2101 attttctgat cgtcgcgttc cgaatcacgc tgcgcggcag tttgaccaca tccaggtcaa
     2161 atgcctcatt gatgctgaat tgtacgtact tgatgttact gatcgggaag acgagaaaac
     2221 tgccatcagc ttcgatcagt acatggtcgc cggtcagaac gtcttcaatg cgcaggtttt
     2281 tgccggcgac gttggtggtc tgctccggga aatcgaaagt gattttggtg ccatcggtaa
     2341 aatgcagggt aatcgatctt ttacgttcca tcatgtcctc ctcgagtttc gggttgggta
     2401 tctattgcgg aacgaccgtc gcgttcatac cggcgccttc gacatagacc ggctggccga
     2461 cgtaaaggtt ggcattggtc ggctgcgaca ctaccaccag tacgccactc ctgaggcgga
     2521 ccacgatctg ttggcccggc tgcttgtcat gcttgttctg caggtagttg ccaccgacgg
     2581 cgccaccgat ggcaccgact gcaattgcca cgtccttgcc ggtgccctgg ccgatcagac
     2641 tgccgatacc ggcgccggcg atgccgccga tgatggcacc cagtccgggg tggttattct
     2701 cgttgatttc tatttcagta atctgttcga tcttgccctg gcggatctcg gcgggctgcg
     2761 acgggctgcc gccgggactg gcacaaccgg ccagcatggc gagaaatgaa acggcaagca
     2821 gcagcgagcg atagtgctgt ctcatgatga ttccttgctg agtaaaaatt aggtgtgact
     2881 gcattgccgg cctgggctag gaattttccg ctttctgctc gttttccagc gccaggcggg
     2941 tgatctcgtt gtacatgccg atgatcttgg cgaccagagg ttggaattcg ggatggcgct
     3001 cgtataaagt tcgcatgcct atgaatccgg agaaagtttc gtcaagaaaa tgcaaatcga
     3061 gctcattgag tttctcgccg cgctgcagcc gttccttcag ttcgagcgca cgcggcagct
     3121 tttgttcgtt gaacctgtcc agtaaagcct ggatgactcc tgcatcctga tctgcatcgg
     3181 ccatgattac ccccttaatt tagaaaccat gaacacgata atataacaaa gtggcgcact
     3241 atgctaagcg cgcgctgaac tgccgccttt tcagcaccgc cctaattgag gcggcggtag
     3301 gcggtcgggt caggtacgcc cgcctgctga aagccggtcc gccggaaacg gcaggagtcg
     3361 cagacgccgc aggcgcgtcc ttcattatcg gcctggtagc aagataccgt catcgcgtag
     3421 tccactccca gcgcgtaccc gcgctggatg atctccgcct tggtgagatc gatcagcggt
     3481 gtgtgcaggc gtagcttgct gccttccacc gcggcccggg tagcaaggtt ggccatgcgc
     3541 tcgaaagcgt cgatatattc gcgccggcag tcgggatagc cggaataatc cagcgcgttg
     3601 acgccgataa agatgtcgag cgcgcccagc acctccgccc aggccagagc cagcgacagc
     3661 atgatcgtgt tgcgcgcggg aacgtaggtg accgggatgc cctcggtcgg cgcttgcggc
     3721 acggcgatac gggcatcggt gagggcggag ccgccgaagg cggcaaggtc gagatggaac
     3781 acacgatgct ccacggcgcc gagtgcctgc gtcactcttt cggcggcctg cagttcagcc
     3841 acgtggcgct ggtggtagtc aaagctcagg cagtagcagg catagccctc gtttctggca
     3901 atagcgagcg cggtggcgga atccaggccg ccagacagca ataccaccgc ctttttcatc
     3961 gtccaggctc ctcgccccac agcagcttgt gcaactgcaa ctggaagcgc accggcaggt
     4021 ggtcggcgag tatccattcc gccaactcgc gccctttgag ctggccgtaa accggtgaaa
     4081 acagcaccgg gcatttggcg acgatggcgc gatcggccag cacccgcctt gcccagtcgt
     4141 agtcctcgcg gctgcacagc acgaacttga cctcgtcctt tggtttgagc aggtcaagat
     4201 ttttccacag gttctttccc gcttcgtcgg agcctggggt cttgatatcg agaatgacgg
     4261 aaacacgcgg atcgacgccc gcaatatcca ggctgccgcc ggtttccagc gagacgtcgt
     4321 aaccggcgtc gcacagccgg ttcagcagcg gcaggcaatc tttttgtgcc agcggctcgc
     4381 cgccggtgac ggtgatatgc cgcgcaccat ggctggccac gcgcttgagg atttcatcca
     4441 gctccagcga ttcaccgccc tggaaagcgt aggcggtgtc gcaatagccg cagcgcagcg
     4501 ggcagccggt caggcgtacg aataccgtgg gcaagccgac acgcgaggcc tctccttgca
     4561 gggagaagaa tatttcgtgg atcctgaggg acaatttgcc gcgaacgcgc ctaattcttg
     4621 gatttgagcg actcgatcgc cgacaggcgc ttcttgccca acgctgcggc gcggctctgg
     4681 ggatacttgg cgatcagtgt tttcagcgtc tggcgggcac cgtccacatc ggccaactgg
     4741 atctggctgt tggcgatgtt aagcagggcg tcaggcgctt tttcgtgatc cggatactgc
     4801 ttcaccagac tctgctgggt ggcgatggcg gacttgtaat cacgcaggga gaattgcgaa
     4861 gttcccgtcc agtagtaggc gttgggaacc agcttgccgt tagggtaatg ctcggtaaac
     4921 tgctggaatg ccgccaccgc ttcggcgtgc ttgccagcct tggtcagcgc gtaggcggca
     4981 tcgtattcct tctgctcgtt ttcggtattg ctcgccatgg ttgccgacgt ggcggcctgg
     5041 gccgcagctt gtgctggcgc gccagcggct gcctccgttg cggtagcggc gccgctttcc
     5101 agcttgcgca gacgtccatc ggtatcggtg tagagatcac gctggcgttt ttgcgtgacc
     5161 tcgacgtcat gtgccgtcac ttccatctgc cccttgagct tgctgatttc ggcgttcaga
     5221 cgctcgatct ggttgagcag atccatgccc tggttcttga gctggttttc cagcgcgacg
     5281 acacgttcat ccgtggcctg gcgctgtttg gcttcctgat ccagcttacc ctgcaactgg
     5341 ctcacctgct gctgaagttc ggtgattttc ttgcgcgctt cgtcatcgct gaaaaacgcc
     5401 gcctgggcgg catggatgcc gcccagcaac aggcaaaaag ccagaaccag tggtttcatc
     5461 aagccttact cgccttcgta aacgatatcg acacggcgat tttgcgacca gcaagtctcg
     5521 gtctgttcgg tgcagcgcgg tttttcttca ccgtagctga cggtctcgat ctgcttgtcg
     5581 ctggcgttga gcaggttcag cgcctttttc accgaggccg cacgacgctg gccgagcgcc
     5641 aggttgtact cgcgcgtgcc gcggtcatcg gcgttgccct gcagcaccac ttttgcgttg
     5701 ggatgcgaag tcaggtactt ggcatgggct tccaccagcg gcctgaattc cggcttgact
     5761 tcatccttgt cgaaatcgaa aaaaatgctg cgtttggaca ggatgttggc ggggtccgta
     5821 agcggattga ccgccaattt cgattgatct accggcttgg tttgtgccga gtcgtccgtg
     5881 gattttcccg ccggcttcgg cgcggactgt gtcgtgtcct tgttttccac gggcgcagcc
     5941 ggctccggct tcacttgctt gctggaacaa gcggctagaa agagcgccag tactatggcg
     6001 ccgataattt gtttgttcat cattgctctc ctaaagttgt gttgctgaaa aaattacttt
     6061 gccgcggggc tccaggaagg ctcccgcacg tctgctgcct cggttagtcg ttgcttgacg
     6121 cgaccatcgg cggaaaccac cgacaataca cccttgccgc ggatactggt ggcaaaaata
     6181 atcaggcgcc cgtttggggc gaagcttggc gattcgtctt gtgccgagtc ggacagtacc
     6241 tgcacctggc cggaagccag atcctgcacc gccacctgga acttgccgcc gtcacgacga
     6301 atgtaagcaa gttgcttgcc gtctggtgat agatgcggac ttgcattata gctgccttca
     6361 aaggtcacgc gctgcgcatc gccgcccagc ggcgacatgc ggtaaatttg cgggccgccg
     6421 ccacgatcgg aggtgaaata gagccacttg ccgtccggcg accagtccgg ctcggtttcg
     6481 atggcacggt tgccggaaag ctgctgcaac ccggtgccgt cggcattgat cagatagacc
     6541 tgcgaattct ccccgtgcgt cagtacgatg gccagccgcc tgccgtctgg agaccacgcc
     6601 ggcgcgctgt tgttgccctt gtagttggcc agcaccgtgc gctggccgga aaccagcgac
     6661 tggacgaaga tcaccggctt ctttttttcg aacgatacat acgccagctt ggtgccatcc
     6721 ggcgaccatg ccggcgagat gatcggttcg ttgctggcca gcacggtctg cggattgtag
     6781 ccatcggcat ccgccaccac cagggcaaaa cgcttgccgt ttttggtgac gtaagtgatg
     6841 cgggtggcga acacgcccgg ctcgccggtc agcttttcgt agatgatgtc ggcgatcttg
     6901 tgcgcggtct ggcgcagctg cgccggcgtg acgtcgtatt ccagcccggc cagctggcgg
     6961 ggcgggcatc cggggcggag agaatggcgc cgatcagtcc gaaaagcgag gcgccgacca
     7021 ggagcccgct gccggccgat gccagcatcc aggccaggat ggcgatgggg cgtcgcacgg
     7081 cggcgggggg cgggtgaggc gcgctgggtc aattcgagcc tctagccatc cg
//
LOCUS       DAOMFA010000082         7085 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_1294_length_7085_cov_4.636558, whole genome shotgun sequence.
ACCESSION   DAOMFA010000082 DAOMFA010000000
VERSION     DAOMFA010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 7085)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7085)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7085
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1294_length_7085_cov_4.636558"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            97..636
                     /locus_tag="PKW44_05395"
     CDS             97..636
                     /locus_tag="PKW44_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR51053.1"
                     /translation="MDHFESIISTLLEAEGYWVRRSFKVNVTKEEKRQIGKHSIPRPE
                     IDLLALNFSKNEVLALEAKSFLDSPGVNLEQLREEHEVPEGRYKLFTSERYRTVVFSR
                     LLSDLIACGMANSKTKIVLGLAAGKVYQGRSGLIREFMSGKNWFFWSPEDIKQKVVAL
                     AERGYENDPAIITAKILMR"
     gene            complement(793..1386)
                     /gene="rnhB"
                     /locus_tag="PKW44_05400"
     CDS             complement(793..1386)
                     /gene="rnhB"
                     /locus_tag="PKW44_05400"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HQR51054.1"
                     /translation="MNVGHCILCGVDEAGRGPLAGAVYAAAVILDPLRPIVGLADSKT
                     LSKSKREVLAAVIRRDALAWAIASASVEEIDRINILQASLLAMRRAVEALSVAPGKVL
                     VDGLHCPPVACPAEAVVKGDSKVAEISAASILAKTARDEEMRQLDALYPQYAFARHKG
                     YPTAEHMRLLLQHGVSPVHRRSYAPVRKLLCESMKGG"
     gene            complement(1383..2519)
                     /gene="lpxB"
                     /locus_tag="PKW44_05405"
     CDS             complement(1383..2519)
                     /gene="lpxB"
                     /locus_tag="PKW44_05405"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HQR51055.1"
                     /translation="MPTIGIVAGEASGDLLGSHLIKSLKQHRPDLQFVGVAGPRMQAE
                     GAVSLFPMEKLAVRGLLEVLRHLPSILGLRRQLVKHFRQNPPDLFIGIDAPDFNLGLE
                     RKLKRRGIKTIHYVSPSIWAWRRGRVNKIRRAVSHMLVLFPHEFPLYEQAGIPATYVG
                     HPLADSLPLEPDREGARRELKLPADKLVIAMLPGSRQSELHYMADLFVKTALQIHQQI
                     ENVQFLVPVISRETRWMFEEVLHRNNADQLPLSTLFGHAHLAMEAADVVLAASGTATL
                     EAALLKRPMVIAYRVSQLSWQIVRHMLYLPFIGLPNILAGRKLVPELLQKDATPEKLA
                     EALMQLVHDKQAVQEIQREFTALHESLRQNTEEKAAQAVLKYLP"
     gene            complement(2529..3299)
                     /gene="lpxA"
                     /locus_tag="PKW44_05410"
     CDS             complement(2529..3299)
                     /gene="lpxA"
                     /locus_tag="PKW44_05410"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HQR51056.1"
                     /translation="MIHSTAIIDPRAELAADVEVGPYSVIGADVRIGSGTRVGSHVVI
                     QGPTTIGRNNQIFHFCSLGEAPQDKKYRGEPTTLEIGDGNTIREYCTLNRGTTQDAGV
                     TRVGSDNWIMAYVHIAHDCQIGDHTIFANYSGLAGHVQVDDYAILGGGTLVHQFCKIG
                     AHVMTGGATVLFKDIPPYVMAAGQDAQPHGINAEGLKRRGFSPETIAHIKRGYKTLYK
                     SGLTLEEAKLQLAEQARECAEIGLLVDFLQGSTRGIIR"
     gene            complement(3296..3742)
                     /gene="fabZ"
                     /locus_tag="PKW44_05415"
     CDS             complement(3296..3742)
                     /gene="fabZ"
                     /locus_tag="PKW44_05415"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HQR51057.1"
                     /translation="MNSMDIHEILEYLPHRFPFLLLDRVISIELGKEIVAIKNVSINE
                     PFFPGHFPHHPVMPGVLIIEAMAQAAAVLSFKSAGIKTSDDSVYYFAGIDRARFKKPV
                     QPGDQLQLRMTVDRVIRGIGKYKGQALVNGEVVAEADVMCILKTIK"
     gene            complement(3739..4773)
                     /gene="lpxD"
                     /locus_tag="PKW44_05420"
     CDS             complement(3739..4773)
                     /gene="lpxD"
                     /locus_tag="PKW44_05420"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HQR51058.1"
                     /translation="MRSGFSLREIVSQLGGEVVGNADIVVRRMASLSNAGSGDLSFLA
                     DAKYRPLLDNTRASAVVVQSAALTTLPCIVTDNPYAYFARVSTLLNPQTPAGLGVHPG
                     AFVDASTTVPASASIAAGSVIGSNVQIGENVVIGPGCAVGNNVVLGDHCRLYANVTIY
                     DRCELGRRCIVHSGVVIGADGFGYAQDGGKWLKIPQVGRVLIGDDVEIGANTTIDRGA
                     LDDTLIGEGVKLDNLIMVAHNVQIGAHTAVAACAGIAGSAKIGRNCRIGGSTNIVGHL
                     EIADGVTVTSCSMITKSLDKAGTYTGIMPFQTHDKWLRTAVNIRNLDKMLERITQLEH
                     EIEALKKGAS"
     gene            complement(4790..5269)
                     /locus_tag="PKW44_05425"
     CDS             complement(4790..5269)
                     /locus_tag="PKW44_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HQR51059.1"
                     /translation="MAGALAVFSMTASAAELKVGYVQVDKILQDAPQTAESGKKLERE
                     FGPRSQELERMQKQIKDLETSLDKDGLTMSESDRRNKERDIAERKLEFQRKQRELRED
                     INLRKNEELSGLQDRINKAVTKVAETEGYDLVVYSGVAYANKKIDITDKVLANLGKK"
     gene            complement(5325..>7085)
                     /gene="bamA"
                     /locus_tag="PKW44_05430"
     CDS             complement(5325..>7085)
                     /gene="bamA"
                     /locus_tag="PKW44_05430"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HQR51060.1"
                     /translation="FDEAELLGLFALTTPNWLSWWNKDDQYSKQKLTADLESLRSFYM
                     NRGYLEFNIDSTQVSITPDKRDIYITINLTEGEKYTVTDVKLAGEMLVPEDELRKLII
                     VQPGEIFDRQKITESSKAIGDRLGNDGFAFANVNAVPEVDKEKHTAAFTFFVDPGRRV
                     YVRRISLTGNNRTRDKVLRREMRQLEAAVYDAGKINRSKERLDRLGYFSDVNLETPAV
                     AGTADEVDVNVNVTEKSTGSIMVGAGLSSSEGIVFNFSVNQSNFLGTGNRVNTSVNTG
                     KVNTTYALSFTDPYFTVDGISRGFDLYRRDVDTNSLDVGDYKTSSYGAGVNFGIPLSE
                     GDRFTAGLTMDFTDINLSANSPQNYINFCNSNSGCTNNSLLNDLAWIHDTRDNVLYPN
                     RGVLQKLSSQIALPVGLDAEYYKLGYQHTWFKDVRKNVVVMLNGEAGYGNSYGNGQFP
                     FFKNYFVGGVSTVRGYQTSSIGPRELDTDGQDYAVGGTKKVVGNAEILVPVPFIKDSK
                     QFRLSAFLDAGSAWGNESSADLAPDSCSGMSDCMRYSTGVGVSWYSPFGPIRVYLAKP
                     LNEKTGDQTQFFQFQMGSQF"
BASE COUNT         1426 a         2095 c         2030 g         1534 t
ORIGIN      
        1 gctcatgctg tcgaaccttc cgctccagag ggacgctccg ccggcaagcc ggctccgcgc
       61 ccctgagcta gaacgttatg tttcagtgag ccgctgatgg atcatttcga gagcatcatt
      121 tctacactgc ttgaagccga gggctattgg gttcgtaggt cattcaaggt caacgtgacg
      181 aaggaagaaa aacgtcagat cggaaagcac tcaattccta ggccggagat tgatctcctt
      241 gcgcttaatt tttccaagaa cgaagtgctt gcccttgaag cgaagtcatt tcttgattcg
      301 ccgggcgtaa atctcgaaca actacgggaa gagcacgaag tcccggaggg gcgatacaag
      361 ctttttacct ccgagcgcta tcgcacggtc gtcttctcgc ggttgctttc agatcttatc
      421 gcctgcggca tggcgaattc caaaacaaag atcgtccttg gccttgctgc tggcaaggtc
      481 taccaaggcc gaagtgggct aatcagagag ttcatgtcgg gtaagaactg gttcttttgg
      541 tcgccagagg acatcaaaca aaaagttgtg gctcttgcgg aacgcgggta tgagaacgac
      601 cctgccatca tcactgccaa gattcttatg cgatgaaaca taaccctgcg ctcaaccgcg
      661 ctcccttcgg tcgctggacg ctgcgcgata aagccgcgca gcgccggtta gctctacgtt
      721 agtgttcatg agccgcactg agtttcgggg tcaggtcttg caaaaacaca ctccaatacg
      781 ccccggcccc tttcagccac ccttcataga ctcacaaagc agcttcctca ccggcgcgta
      841 gctgcggcgg tggacggggg aaacgccgtg ctgtaggagc agccgcatgt gttccgcggt
      901 gggatagccc ttgtgtcggg cgaaggcgta ttgcgggtaa agcgcgtcga gctggcgcat
      961 ttcctcgtcg cgcgcggttt ttgccaggat ggaggcggcg gagatctcgg cgaccttgct
     1021 gtcgcccttg actacggctt ccgccgggca ggcaaccggc ggacagtgca ggccatccac
     1081 cagcaccttg ccgggcgcaa cggataatgc ttccaccgcg cgccgcatgg caagcaggct
     1141 ggcctgcagg atattgatgc ggtcgatttc ttccacgctg gcgctggcga tggcccaggc
     1201 cagggcatcg cgccggatga cggcagccag cacttcacgc ttgcttttgg acagggtttt
     1261 ggaatcggcc aggcccacta tcggtcgcag cgggtcgagg atcacggcag cggcatagac
     1321 ggcgccggcg agcggccccc ggcccgcctc gtccacgccg cacaggatgc agtgcccgac
     1381 gttcacggga ggtatttcag gacggcctgg gcggcctttt cttcggtgtt ctgccgcagc
     1441 gattcgtgga gggcggtaaa ctcgcgctgg atttcctgta ctgcctgttt gtcatgcacc
     1501 agttgcatca gggcttcggc cagtttttcc ggcgtggcgt ccttctgcag cagttccggc
     1561 acgagcttgc gccccgccag gatgttgggc aggccgataa aaggcaggta aagcatgtgg
     1621 cgcacgatct gccagctcaa ttgcgacacg cggtaggcga tcaccatcgg gcgcttgagc
     1681 agcgccgctt ccagcgtggc ggtgccggac gcagccagca ccacgtccgc cgcttccatg
     1741 gccaggtggg cgtggccaaa caatgtgctg agcggcagtt gatccgcgtt gttgcggtgc
     1801 agcacctctt cgaacatcca gcgcgtttca cgcgaaatga ccggcaccag gaactgtacg
     1861 ttttcgatct gctggtggat ttgcagcgcg gtttttacaa acaggtcggc catgtagtgc
     1921 aattcgctct ggcggctgcc cgggagcatg gcgatgacca gcttgtcggc aggcagcttg
     1981 agctccctgc gcgcaccctc gcgatccggc tccagcggca ggctgtccgc cagcggatgt
     2041 ccgacatagg tggcaggtat cccggcttgt tcgtaaagcg gaaactcgtg cgggaacagc
     2101 accagcatgt gggatacggc tcgcctgatc ttgttgacgc ggccacggcg ccaggcccag
     2161 atggaggggc tgacataatg aatggtcttg atgccgcggc gcttgagctt gcgctccagc
     2221 cccaggttga aatccggtgc atcgataccg ataaacagat cgggcggatt ttgccggaag
     2281 tgcttcacca gttggcgccg caagccgaga atggaaggca ggtggcgcag cacctcaaga
     2341 aggccgcgca cggccagctt ttccatgggg aacagcgaaa ccgcgccttc cgcctgcatc
     2401 ctggggccgg caacaccgac gaactggagg tcgggacggt gctgcttgag ggatttgatc
     2461 aggtggctgc caagcagatc gccggaggcc tctccggcca cgataccgat agtgggcata
     2521 acgaaaacct agcggatgat gccgcgcgtg gaaccctgga gaaagtccac cagaaggccg
     2581 atctcggcac attccctggc ctgttcggca agctgcagct tggcttcttc cagagtcagc
     2641 ccggatttgt agagggtctt gtagccgcgc ttgatgtggg cgatggtttc aggcgaaaac
     2701 ccccggcgct tcagcccttc ggcgttgatg ccgtgcggct gcgcatcctg ccccgcggcc
     2761 atcacgtagg gggggatgtc cttgaacagc accgtagcgc caccagtcat cacgtgggcc
     2821 cctatcttgc agaactggtg caccagggtg ccgccgccga gaatggcata gtcatccacc
     2881 tgcacatgcc cggccaggcc ggaatagttg gcgaagatgg tatggtcgcc aatctggcaa
     2941 tcgtgcgcaa tgtgcacata ggccatgatc cagttgtcgc tgccgacccg ggttacgccc
     3001 gcatcctgcg tggtgccgcg gttcagcgtg caatactcgc gaatggtgtt gccgtcaccg
     3061 atctcgagcg tggtcggctc gccccggtac tttttgtcct gcggggcttc gcccagcgag
     3121 cagaaatgga aaatctggtt gttgcggccg atggtggtcg gcccctggat caccacatgc
     3181 gacccgacgc gggttccgct gccgatgcgc acatcggcgc cgatcacgga ataagggccg
     3241 acctcgacat cggctgccag ttcggccctg gggtcaataa tggcggtgga atgaatcatt
     3301 tgatcgtttt cagaatgcac atcacatcag cctcggcaac cacttcgccg ttgaccagcg
     3361 cctgcccctt gtacttgccg atgccgcgaa tgacccgatc caccgtcatt ctcagctgca
     3421 gctggtcgcc cggttgcacc ggcttcttga agcgtgccct gtcgatgccg gcaaaatagt
     3481 agacggaatc atcggaggtc ttgatcccgg cgctcttgaa cgaaagcacg gcggccgcct
     3541 gggccatggc ctcgataatc agaacaccgg gcatgaccgg atgatgagga aagtggccgg
     3601 gaaagaacgg ttcgttgatg ctcacattct tgatagcgac aatctctttt ccgagttcaa
     3661 tggatatcac gcggtccagc agcaggaatg gaaagcggtg cggcagatat tcaagtatct
     3721 cgtggatgtc catgctgttc atgatgcccc ctttttcaac gcttcaattt catgttcaag
     3781 ttgtgtaatg cgttccagca ttttatccag attccgaata ttgacggccg ttctcagcca
     3841 cttgtcgtgg gtctggaatg gcatgatgcc ggtgtaggta ccggccttgt ccagcgactt
     3901 ggtaatcatg ctgcaggacg tcaccgtcac gccatcggct atttccagat gccctacgat
     3961 attggttgac ccgccgatcc ggcaattgcg gccaatcttg gcgctaccgg caataccggc
     4021 gcaggcagca acggcagtat gtgcgccaat ctggacgttg tgtgcgacca tgatcagatt
     4081 gtccagtttg acgccttcgc cgatcaaggt atcgtccagc gcgccacggt cgatagtggt
     4141 gttggccccg atttccacat cgtcgccgat cagcactcgg cccacttgcg gaatcttcag
     4201 ccattttccg ccgtcctggg cgtagccgaa cccgtcggcg ccaatgacca cgccggaatg
     4261 tacgatgcag cgccggccca gttcacagcg gtcataaatg gtgacattgg catacagccg
     4321 gcaatggtcg ccaagcacca cattattgcc aactgcacac ccgggcccga tgacgacgtt
     4381 ctctccaatc tgcacgttgc tgccaatcac gctgccggca gcgatactgg ctgaagcggg
     4441 gacggtcgtg gaagcatcga cgaaagcacc ggggtgcaca ccaagcccgg ccggcgtttg
     4501 cggattcaac aacgtcgaca ccctggcaaa ataggcatag ggattgtcgg tcacgataca
     4561 gggcaacgtc gtcaatgccg cagactgcac gaccacagcg ctcgccctgg tgttatccag
     4621 caaaggacgg tattttgcat ccgccagaaa gctcaggtcg ccgctcccgg cattgcttaa
     4681 cgaggccatg cgccgcacga cgatatcggc attgcccacc acttcgccgc ccagctgcga
     4741 cactatctcc cgcagagaaa atccgctacg catcagaggc agctatgact tatttcttgc
     4801 ccagattggc gagaaccttg tcggtaatat cgattttctt gttggcgtag gcaacgccac
     4861 tatagaccac cagatcatag ccttccgttt ccgccacctt ggttaccgcc ttgttgatgc
     4921 gatcctgcaa cccggacagc tcttcgttct tgcgcagatt gatatcctcg cgcaactcac
     4981 gctgcttgcg ctggaactcc agtttgcgtt cggcaatatc gcgttccttg ttgcgccgat
     5041 cgctttcgga catggtcaaa ccatccttat ccagactggt ttccagatcc ttgatctgtt
     5101 tttgcatgcg ttcgagctcc tgcgagcggg gaccgaactc ccgctccagt ttcttgccgc
     5161 tctcggcggt ttgcggcgca tcctgcagaa tcttgtcgac ctgcacgtac cctaccttga
     5221 gttcggcagc cgaggcggtc atggaaaaca ctgccagcgc acctgccatt aagtaatgcg
     5281 aaaatttgtt caattggctt ctcctaaaat tcatgattga ttcgttagaa ctgtgacccc
     5341 atctggaact ggaagaactg ggtctgatcg ccggtctttt cgttaagtgg cttggccaga
     5401 tacacccgga tcggtccaaa cggtgaatac cagctcaccc ctacgccagt cgaatagcgc
     5461 atgcagtcgc tcatgccact gcaggaatcc ggcgcaagat cagcactgct ttcattgccc
     5521 caagccgagc cggcatccag aaacgccgac agccgaaact gcttcgaatc cttgataaac
     5581 ggcactggca ccaggatctc ggcattgcct accacctttt tggtgccgcc aacggcataa
     5641 tcctgcccgt cggtatccag ttcgcgcggc ccgatggagc tggtctggta gccacgaaca
     5701 gtactcacgc caccgacaaa gtagttcttg aagaagggga actgcccgtt accataactg
     5761 ttaccgtaac ccgcttcccc attcagcatc accaccacgt tctttctgac atccttgaac
     5821 caggtatgct ggtaacccag cttgtaatac tcggcatcca gcccgacagg cagcgcgatt
     5881 tgactggaca gcttctgcaa cacgccacga ttcgggtaca gcacgttatc gcgcgtgtcg
     5941 tgaatccatg ccaggtcgtt cagcaggctg ttgttggtac agccgctgtt actgttacag
     6001 aaattgatgt aattctgcgg gctgtttgcc gacagattaa tatcggtgaa gtccatcgtc
     6061 agcccggccg tgaaccggtc gccctcgctg aggggaatgc caaagtttac accggcacca
     6121 taggatgaag ttttgtagtc accgacatct agggaattgg tgtccacatc ccggcggtac
     6181 aggtcgaaac cgcggctgat gccatctacc gtaaagtagg ggtcggtaaa cgacagcgcg
     6241 taagtggtat tgaccttgcc agtattgacc gaggtattga cccggttgcc ggtgcccagg
     6301 aaattggact ggttgacgct gaaattgaac acgatacctt ccgagctgga cagcccggcg
     6361 ccaaccatga tgctgcccgt ggacttctcg gtgacgttga cgttgacgtc cacctcgtcc
     6421 gccgtcccgg caacggccgg cgtctccaga ttgacgtcgg agaaataccc caaccgatcc
     6481 aggcgctcct tggagcggtt gatcttgccc gcatcgtaga cagcggcttc cagctggcgc
     6541 atctcccgac gcagcacctt gtcgcgggta cgattgttgc cggtcaggct gatgcggcgc
     6601 acatacacgc ggcggccggg gtcgacaaaa aatgtgaaag cggccgtgtg cttttccttg
     6661 tccacttccg gcactgcatt gacgttggcg aaggcaaagc cgtcattgcc cagacggtca
     6721 ccaatcgcct tgctggactc ggtaatcttc tggcgatcga atatctcccc cggctgcacg
     6781 atgatgagtt tacgcagctc gtcttccggc accagcatct cgcccgccag cttgacgtcg
     6841 gtcacggtgt atttctcacc ctcggtgagg ttgatggtga tgtagatgtc gcgcttgtcc
     6901 ggagtaatgg atacctgtgt ggaatcaatg ttgaactcca gataaccccg attcatgtag
     6961 aaggagcgca atgattccag atcggccgtc agcttctgct tggaatattg gtcgtccttg
     7021 ttccaccagc tcaaccagtt gggcgtggtc agggcaaaca ggccaagcag ctcagcctcg
     7081 tcaaa
//
LOCUS       DAOMFA010000083         7026 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_1311_length_7026_cov_4.669201, whole genome shotgun sequence.
ACCESSION   DAOMFA010000083 DAOMFA010000000
VERSION     DAOMFA010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 7026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7026
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1311_length_7026_cov_4.669201"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..256)
                     /locus_tag="PKW44_05435"
     CDS             complement(<1..256)
                     /locus_tag="PKW44_05435"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018508727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HQR51061.1"
                     /translation="MSQETVFADLGLAQPLLRAIADVGYTTPTPIQAKAIPLVLQGGD
                     LLAGAQTGTGKTAGFTLPILQLLSENPVTLKAGRPRCLVLV"
     gene            547..1647
                     /locus_tag="PKW44_05440"
     CDS             547..1647
                     /locus_tag="PKW44_05440"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HQR51062.1"
                     /translation="MSAQQDISQEKLTPRNLLDVLIRAGMVAVLVVLCFQIFAPFLNM
                     MLWALILAVALYPLHRMIAARIGGRQGVAATLLVLVGLVLIVAPAILLTSSMAEWVKG
                     LVEGVRQNGIVIPPPTEAVAAWPLVGGKIYGLWQQAATDLPGLLQTLHLNYGVLAKSA
                     LGFAAGMGGSMLVFIFSLIVAGIIMAYGESGSKAAVDIANRFVGKKKGPALAALSTAT
                     IRSVAQGVIGVAFIQALLVGIGLVWAGIPAAGLLAIILLVLGIAQLPALIITLPAIAY
                     IWMVGGHSSLMAVLMTVYLIAAGMADGFLKPVLLGRGVDAPMPVILLGALGGMVWAGI
                     IGMFVGAVFLALGYQIFMAWVHEGEEVNAASK"
     gene            complement(1720..2457)
                     /locus_tag="PKW44_05445"
     CDS             complement(1720..2457)
                     /locus_tag="PKW44_05445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR51063.1"
                     /translation="MKKLGLALLVLIVIVASALLWLRGNLDGLVRDAIAKYGSEMTQA
                     RVSVDGVKISAASGKGIISGLAIGNPKGFKTPRALNIQEFTVAIDPATIAGDVVTVKK
                     IAIIAPNVVYEKGETMTNFDAIQNNIANYLGPSDNKTSEGGKKLIVEKLTIRGARAEV
                     SAAFMDGKTVTVDLPDLTLRNMGKAKGGITPGELGQEIAGALQKQLAHAVNFDKIGKA
                     IGESASATAKEAAEASGKAVDKVKSLF"
     gene            complement(2486..3136)
                     /locus_tag="PKW44_05450"
     CDS             complement(2486..3136)
                     /locus_tag="PKW44_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HQR51064.1"
                     /translation="MLLPASLQLAAVKRVKAVLFVIALLPLARLLVLGFADQLGANPI
                     EFVIRSNGTWALSFVLITLAVTPLRRLAGAGWLAGLRRMLGLYAFFYACLHVLSYIWL
                     DQWFDWAAIGKDIVKHPFILAGFTAFLLLVPLAVTSTDAMLRRLGKRWKKLHQTLYLI
                     APIAVLHYFWLVKKDITQPVIYALLLALLLGLRLLWKVLERPGRHSRLLQKSPIIR"
     gene            complement(3136..4107)
                     /gene="msrP"
                     /locus_tag="PKW44_05455"
     CDS             complement(3136..4107)
                     /gene="msrP"
                     /locus_tag="PKW44_05455"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HQR51065.1"
                     /translation="MLIRSLPDIAPSEIAPREVYEGRRHFLGQAGGIALAGATLGLLG
                     ENALAAAPATSGQKIAGIVKTRYGMEEKPTPYEDVTSYNNFYEFGTGKADPAEYAHTL
                     RTRPWTVGIDGEVKKPKRIAIEDIYKLAPLEERLYRMRCVEGWSMAIPWVGFSLAELI
                     KWAEPTGNARFVEFISLADQQQMPGVRSTVLDWPYNEGLRMDEAMHPLTLLAVGLYGE
                     VLPNQNGAPLRLVVPWKYGFKGIKSIVRIRFTEQMPVSSWMKANAREYGFYSNVNPDV
                     DHPRWSQATERRIGTGLFQRRQKTLMFNGYAEQVAQLYTGMDLRKNF"
     gene            4281..5387
                     /gene="hisC"
                     /locus_tag="PKW44_05460"
     CDS             4281..5387
                     /gene="hisC"
                     /locus_tag="PKW44_05460"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HQR51066.1"
                     /translation="MSLSDLAPDYVRSIAPYQPGKPISELARELGLQESSIVKLASNE
                     NPLGVSPKAKIAMRAALEDVALYPDGNGFALKDAIKRKLGVGHDRIVLGNGSNDVLEL
                     AARAFLAPSLEAVYSQHAFAVYPLVTQAIGATGICTPARDFGHDLAAMRAAVAPKTRL
                     LFIANPNNPTGTLLDKTELHAFLGDLPRDVLVVLDEAYDEYLADDLKSDSIAWLQEFD
                     NLIVTRTLSKAYGLAGLRVGFALAHPQVADMMNRVRQPFNVNSVAQAAALAALADDDF
                     VRRSRDLNDAGMAQVTSGFAHLGLNYIPSFGNFVSVCVGDAAAIYRRLLGKGVIVRPV
                     AGYEMPQYLRVSIGLESENARLLRALEESLALGS"
     gene            complement(5411..6286)
                     /locus_tag="PKW44_05465"
     CDS             complement(5411..6286)
                     /locus_tag="PKW44_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HQR51067.1"
                     /translation="MPIKSRTRNSKPAKAGKADSAPGIIKIAGIQMASGPQVSANLSE
                     AERLIEIAVAQGAKLVALPEYFAIMGMKDTDKIKACEEEGNGPIQRFLSRMAKKHQIW
                     LVGGSVPLKSSSPKKVYNSCLVFDPSGKAAARYDKIHLFNLDLGNEHYHEDDTIEPGE
                     GVVVLKTPFGLLGLSICYDLRFPELYRAMGEVDLIVVPSAFTDTTGKAHWETLVRARA
                     IENLCYVLAPAQGGYHLSGRETHGNSMIVDPWGVVLDRLPRGSGVVIAGINPAYQASL
                     RQSLPALKHRKLHAC"
     gene            complement(6333..>7026)
                     /locus_tag="PKW44_05470"
     CDS             complement(6333..>7026)
                     /locus_tag="PKW44_05470"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HQR51068.1"
                     /translation="GKLQNWKSNPATSFNLTWDISDIGKTMERYGKTDAIRGGKGTIS
                     GQLKWPGPIHAYDASILDGTLDLTAEKGQILKINPGVGRLFSILSLQNLPRRLTFDFR
                     DVFSEGFSFDKIDSRLIARQGVVQIDKFQMDGPVAKVEMKGETNLVKETQNLHVKVTP
                     AISDSLSLAAFAGGPAVGAAAWLAQKILRGPADKLAAYEFDVTGTWADPQEVSSKKTP
                     IEPAPGVAPFGN"
BASE COUNT         1258 a         1966 c         2251 g         1551 t
ORIGIN      
        1 ggacgaggac gaggcagcgc gggcgtccgg ccttgagtgt tacggggttt tcgctcagca
       61 gctgcagaat cggcagggtg aagccggcgg tcttgccggt gccggtctgc gcgccggcca
      121 gcaggtcgcc gccttgcagt accagcggga tggccttggc ctggatcggg gtgggggtgg
      181 tgtagccgac atcggcgatg gcgcgcaaca agggttgtgc cagaccgagg tcggcaaaga
      241 ctgtttcttg tgacaagtgt aatgctcctg cgacggcctg ccgttcacgc tgaacagcac
      301 caatcgaggc agacgaatta agaggtcgaa agaccaggtg agaaaaatga ccggtacgct
      361 gattggtggg cgtacggaag cggcgaatta tacgcagtcg gcaaataaaa gccagcgcgt
      421 tgttgcgggg cgtcccggat cgttgttagc ttgaccgctg ggatggaaga attgatactc
      481 ccagatggtt gaaatgcatg cttttcaaat agccgcctga ccttaacgca tcaatggaga
      541 aaaacaatga gtgcccaaca agatatttcc caggagaaac tgacaccacg caacttgctg
      601 gatgtgctca ttcgcgccgg catggttgcc gtgctggtag tgttgtgctt tcagatattc
      661 gcgccgtttc ttaacatgat gctgtgggcg ttgattctgg cggtggcgct ttacccattg
      721 catcggatga ttgcggccag aatcgggggg cggcagggtg tggcggcaac cctgctggtg
      781 cttgttggcc tggtgctcat cgtggcacct gcaatcttgc tgacgagctc tatggccgag
      841 tgggttaaag gcctggttga gggagtgcgc cagaacggga tcgtgattcc gcctcctacc
      901 gaggcggtag ccgcttggcc gctggtgggt gggaagatct atggactgtg gcagcaggcg
      961 gccactgact tgccgggcct gctgcagacg ttgcatctga attacggcgt tctggcaaaa
     1021 tcggccctgg gttttgcggc cggcatgggc gggtcgatgc tggtgttcat cttttcgctg
     1081 attgttgccg gtatcatcat ggcctacggt gaatccggct ccaaggcggc ggtcgacatt
     1141 gccaaccggt ttgtcggcaa aaagaaaggt ccggctctcg ccgcgctcag tactgccacc
     1201 atccgttccg tggcgcaggg ggtgatcggc gtcgcgttta tccaggcgct gctggtcggg
     1261 atcggactgg tctgggcagg cattcctgcc gccggtctgc tggcgatcat attgctggtg
     1321 ctcggcatcg cccagcttcc tgcacttatt atcacgctgc cggccattgc ctatatctgg
     1381 atggtgggcg ggcattcctc gctgatggcg gtgctgatga cggtttacct gattgccgcc
     1441 ggcatggctg acggtttcct caagccggtc ctgttggggc gcggtgtgga tgcgccgatg
     1501 ccggtcatcc tgctgggtgc cttgggcggc atggtctggg cggggattat cgggatgttt
     1561 gtcggtgcag tgttccttgc cctgggctac cagatattca tggcgtgggt gcatgaaggg
     1621 gaagaggtga acgccgccag caagtagggc tgttttttcc cgccagcctc atcggtcggc
     1681 gcgtcttgct actctcccgc cgtcgtcagg catgggtcgc tagaacaggc ttttgacctt
     1741 gtctaccgcc tttccggatg cttccgcggc ttccttcgcc gtggcgctgg cgctttcacc
     1801 gattgccttg ccgatcttgt cgaagttgac ggcgtgagcg agttgtttct gcagggcgcc
     1861 ggcaatctcc tggcccagtt cgccgggcgt gatgccaccc ttggccttgc ccatgttgcg
     1921 gagagtgagg tcaggcagat cgacggtgac ggtcttgccg tccatgaatg cggcagagac
     1981 ttccgccctg gctccccgga tggtcagttt ctcgacaatc agtttcttgc cgccttcgga
     2041 agtcttgttg tccgatggtc cgaggtaatt ggcgatattg ttctggatgg catcgaaatt
     2101 ggtcatcgtc tcgccttttt cgtagacgac gttcggagcg atgatggcga tcttctttac
     2161 cgtcaccacg tcgccggcga tggtggccgg gtcgatggcc acagtgaact cctggatatt
     2221 gagtgcccga ggcgtcttga aacccttggg attgccgatg gctaggccgc tgatgatacc
     2281 tttaccactg gccgcactga tcttgacgcc gtccacgctg accctggcct gggtcatttc
     2341 gctgccgtac ttggcgatgg cgtcgcgcac cagcccgtcc aggttgccgc gcagccagag
     2401 cagagcgctg gcgacgatca cgatcaggac aagtagggca aggcccagtt ttttcatggg
     2461 gtgctcctcg gtgcggaatg ttggactagc ggattatggg tgatttttgc agaagccgcg
     2521 agtgccgccc tggtctttcc agtaccttcc acaacaacct gagtcccagc aacagcgcca
     2581 gtagcagcgc gtaaatgacc ggctgggtga tgtccttctt caccagccag aaataatgca
     2641 gcacggctat cggggcaatg aggtagagag tttgatgcag cttcttccag cgtttcccca
     2701 ggcgacgcag catggcgtcg gtggaagtca cagcgagcgg aaccagcagc aaaaaagcgg
     2761 tgaacccggc caggatgaaa gggtgcttga cgatgtcctt gccgatggcg gcccagtcga
     2821 accactgatc cagccagatg taggacagca catgcaggca cgcgtagaaa aatgcataca
     2881 gccccagcat gcggcgcaag ccagcaagcc agccagcgcc ggcaaggcgg cggagcgggg
     2941 tcactgccag cgtgatgagt acgaagctca gtgcccacgt gccgttagag cggatgacga
     3001 actcgatggg gttggcgccc agctggtcgg caaatcccag caccagcagg cgtgccagcg
     3061 ggagcagggc gatgacgaag aggacggctt tcacccgttt gacggcggcg agctggagcg
     3121 aggcgggtag cagcatcaga agttcttgcg cagatccatg ccggtataaa gctgtgccac
     3181 ctgctcagca tagccgttga acatcagtgt cttctgccgg cgctggaaca ggccggtgcc
     3241 gatgcgtcgt tcggtggcct ggctccagcg tgggtgatcc acgtcagggt tgacgttgga
     3301 atagaagcca tactcgcgtg cattggcttt catccaggaa ctgaccggca tctgctcggt
     3361 gaagcggatg cgcacgatgg acttgatgcc tttgaagccg tatttccacg gtaccaccag
     3421 ccgtagcggg gcgccgttct ggttgggcag gacttcgccg tacagcccga cggcgagcag
     3481 ggttagcggg tgcatcgcct cgtccatgcg caggccttcg ttgtagggcc agtccagtac
     3541 ggtcgagcgc acgcccggca tctgctgctg gtcggccagc gagatgaatt cgacgaatct
     3601 ggcgttgccg gtcggctctg cccatttgat cagttctgcc agtgagaagc cgacccaggg
     3661 gatcgccatc gaccaaccct cgacgcagcg catgcggtac agacgttctt ccagcggtgc
     3721 cagtttatag atgtcctcga tggcgatacg cttgggcttt ttcacttcgc catcgatgcc
     3781 gaccgtccac ggccgtgtcc gcagggtatg ggcatactcg gcggggtcgg ccttgccggt
     3841 gccgaattcg tagaagttgt tatagctggt gacgtcctcg taaggcgtgg gcttttcctc
     3901 catgccatag cgggttttca cgatgccagc gattttttgt cccgaagtgg cgggcgccgc
     3961 ggccagggca ttctcgccca gaagtccgag cgtggcgccc gccagggcaa tcccgccggc
     4021 ctgcccgagg aaatggcgac gaccctcgta gacctcgcgc ggcgcgatct cggaaggcgc
     4081 gatgtcgggt agggagcgaa tcaacatggt caatgcctca ttcagtgtat acgggaaagt
     4141 gccagcccgg aatcattgcg aaatgatcgc atcccggtta ccgcttggtc gtggtaagac
     4201 cgcaaaactt tacatgtgtt ttaggctaga atgtaaccca tcatgcggcg caagccgcgt
     4261 ttttgtttac ggagtgtgtc ttgagcttat ctgacctggc gccggattac gtgcgctcca
     4321 tcgctcctta ccagccgggc aagccgattt cggaactggc gcgcgaactg gggctgcaag
     4381 agtcttccat cgtcaagctg gcctcgaacg aaaacccgct gggcgtcagc cccaaggcca
     4441 agatcgcgat gcgggcagcg ctggaagatg tggcgctgta tcccgatggc aacggcttcg
     4501 cgctgaagga tgccatcaag cgaaaacttg gcgtggggca cgatcgcatc gtgctcggca
     4561 acgggtccaa cgacgtgctg gaactggccg cacgcgcatt cctcgcgccg agtctggagg
     4621 cggtttattc gcagcatgct tttgccgtct acccgctggt gacgcaggcg attggcgcta
     4681 ctggcatctg tacgccggcg cgtgatttcg gccatgatct tgccgccatg cgcgcggcag
     4741 tcgcgccgaa gacgcgcctg ctgttcatcg ccaaccccaa caatcccacc ggtaccttgc
     4801 tggataagac cgagttgcat gcctttctgg gcgatttgcc cagggacgta ctggtggtgc
     4861 tggacgaggc ttatgacgaa taccttgcgg atgatctgaa atcggacagc attgcctggc
     4921 tgcaagagtt cgataacctg atcgtcaccc gcaccttgtc caaagcgtac ggcttggccg
     4981 gactgcgcgt cggctttgcg ctggcacatc cgcaggtggc ggacatgatg aaccgggtgc
     5041 ggcagccgtt caacgtcaac agcgtggcgc aggcggcagc gttggcggcg ttggcggatg
     5101 atgacttcgt gcgtcgtagt cgcgacctta acgatgcggg catggcacag gtcacgtcag
     5161 gatttgcgca tctggggctg aactacatcc cgtcgttcgg aaactttgtc agcgtgtgcg
     5221 tcggcgatgc ggcagcgatt taccgccggc tattgggaaa aggggtgatc gtacgtccgg
     5281 tggccggcta cgaaatgccg caatacctgc gggtcagcat cggcctggaa agcgaaaacg
     5341 cccgcttgtt gcgggcgctg gaagaaagcc tggcgctggg cagctaagcg ttcaggcaaa
     5401 gattactgcg tcagcacgcg tgcagtttgc ggtgcttgag cgccggcagg ctctggcgca
     5461 ggctggcctg gtaggccggg ttgatgccag caatgaccac accggagccg cgcggcagac
     5521 ggtccagtac cacgccccaa gggtcgacga tcatgctgtt gccgtgggtt tcgcggccgg
     5581 agaggtggta gccgccctgc gccggtgcca gcacgtagca cagattttcg atggcacggg
     5641 cgcgcaccag ggtttcccag tgcgccttgc cggtagtgtc ggtaaatgcg gacggcacca
     5701 cgatcaggtc gacctcgccc atggcgcgat agagttccgg gaagcgcagg tcgtagcaga
     5761 tggaaagccc gagcaggccg aacggggttt tcagcaccac caccccttca cccggttcta
     5821 tggtgtcgtc ttcgtggtaa tgctcattac cgaggtcgag gttgaacagg tgaatcttgt
     5881 catagcgtgc tgccgccttg ccgctggggt cgaacaccag gcaggagttg tagactttct
     5941 tgggtgagga tgacttcagg ggcaccgagc cgccaaccag ccagatctgg tgctttttcg
     6001 ccattctcga caaaaagcgc tggatcggcc cgttgccctc ttcctcgcat gccttgatct
     6061 tgtcggtatc cttcatgccc atgatggcaa agtattccgg taacgctacc agcttggctc
     6121 cctgtgccac ggcaatctcg atcaggcgct ccgcctcgct caggttggcc gacacctgcg
     6181 gcccagaagc catctggatg ccggcgatct tgatgatgcc gggagcggag tcggccttgc
     6241 cagctttggc cggtttgctg tttctggtgc gcgatttgat gggcatgata atttttttgt
     6301 actgcgtttg ttagtgtttg ttaaatgata cctcaattgc caaatggcgc tactcctggg
     6361 gcgggctcga tcggggtttt tttggagctg acttcctgtg gatcagccca ggtaccggta
     6421 acatcgaact cgtaggcagc cagtttgtcg gccggtccac gcaatatttt ttgcgccagc
     6481 catgcggcgg cacccaccgc cggtcctccg gcgaacgctg ccagcgagag gctgtcactg
     6541 atggccggcg tcaccttgac gtgcaggttt tgcgtttcct tgaccagatt ggtctcgccc
     6601 ttcatttcca ccttggccac cgggccgtcc atttggaact tgtcgatttg cacgacgccc
     6661 tgcctggcga tcagcctgga gtcgatcttg tcgaagctga acccctcgct gaacacgtcg
     6721 cggaaatcga acgtcaggcg gcgcggcagg ttttgcaggc tgaggatgct gaacagcctg
     6781 cctacgccgg gattgatctt gagaatctgg cccttttcag cggtcagatc gagagtgccg
     6841 tccagaatcg acgcgtcata ggcatgaata ggacccggcc atttaagttg tccgctgatc
     6901 gtacccttgc cgcccctgat ggcgtcagtc ttgccgtaac gctccatggt cttgccgatg
     6961 tcgctgatgt cccaggtcaa gttgaagctg gtggccgggt tgcttttcca gttctgcaat
     7021 ttgcca
//
LOCUS       DAOMFA010000084         6975 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_1336_length_6975_cov_5.481358, whole genome shotgun sequence.
ACCESSION   DAOMFA010000084 DAOMFA010000000
VERSION     DAOMFA010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 6975)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6975)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6975
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1336_length_6975_cov_5.481358"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..548)
                     /gene="holB"
                     /locus_tag="PKW44_05475"
     CDS             complement(<1..548)
                     /gene="holB"
                     /locus_tag="PKW44_05475"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HQR51069.1"
                     /translation="MKLYPWQKALWGKLTATRERLSHALLLQGREGVGKFDFAALLAQ
                     SLLCENTAADQLACGNCQSCVWFAQGNHPDFRLVEPEEGDANTEDEGAAPRTTRKTQI
                     AVDQVRELTEFLGLSSHRSGLRIVLLHPAEALNQASANALLKMLEEPPSGVLFMLVSH
                     QPQRLLATIRSRCSKVDMPIPPR"
     gene            complement(554..1159)
                     /gene="tmk"
                     /locus_tag="PKW44_05480"
     CDS             complement(554..1159)
                     /gene="tmk"
                     /locus_tag="PKW44_05480"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HQR51070.1"
                     /translation="MAGKFITLEGVDGAGKSTHIPAIAALLRARGNEVLVTREPGGTP
                     LGEKLRELLLHHPMHPETETLLMFAARREHLEQVILPALARGIDVLSDRFTDASFAYQ
                     SGGRGLGENKITQLERWVQQDFQPDLTLLFDVPVSISLERLAGTRDPDRFEREQADFF
                     ERIRRAYLKRAGQFPARFRIIDSADTLENINKRLEEIISTL"
     gene            complement(1159..2160)
                     /gene="mltG"
                     /locus_tag="PKW44_05485"
     CDS             complement(1159..2160)
                     /gene="mltG"
                     /locus_tag="PKW44_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HQR51071.1"
                     /translation="MRLLRRLLVSGLFGLLLLAGGWLYHFASQPLELPRTPYDMVLKH
                     GSSLRGIAQQLVSERVLKEPWSFMLLVRAMGKASEIKAGNYQLEQGITGFALFQMMTE
                     GSTVQNSITFIEGWTFMQMREALNAHESVRHLSMPLTDAQIMEQIGATETMPEGLFFP
                     DSYYFSSGMSDLDILKRAYQTMRQKLDLAWQGRDTGLPYATPYQALIMASIIEKETGR
                     GGERPLIARVFLNRIKLGMRLQTDPTVIYGMGEAFDGNLRKRDLLRDTPYNTYTRDGL
                     PPTPIAMPGQAAIEAALHPARGGALYFVGRGDGSHAFSNSLVEHNNAVARYQLRRRD"
     gene            complement(2144..2971)
                     /gene="pabC"
                     /locus_tag="PKW44_05490"
     CDS             complement(2144..2971)
                     /gene="pabC"
                     /locus_tag="PKW44_05490"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020181881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008696 - 4-amino-4-deoxychorismate lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HQR51072.1"
                     /translation="MQNQFLINGRHDGLTPLDRGFAYGDGVFRTLPVYRGHPHCWERH
                     YHRLSDDCNALGIVCPPAELLLGDITRLCGDDEDAAFKIIVTRGNGMRGYAVPPLAQP
                     NRVLLKSGLPIWPESNFSYGVSLHLCQLALSSQPRLAGIKHLNRLENVLARMEWTDAN
                     IADGLLLDIAGNVIECTMSNVFIRQNDILLTPDLTQCGVAGVTRQRIVELATTLGFEV
                     RVCSFKLDSLLSADEVVLCNSLFGAWQVRELAGKTWPAGELASRLRTLLKEDHAPTA"
     gene            complement(3025..4263)
                     /gene="fabF"
                     /locus_tag="PKW44_05495"
     CDS             complement(3025..4263)
                     /gene="fabF"
                     /locus_tag="PKW44_05495"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HQR51073.1"
                     /translation="MSKRRVVVTGLGIVSPVGIGVANAWADIIAGKSGITRITKFDPS
                     TFSSQVAGEVKGFDVSQYLPAKDARRMDTFIQYGLAAGIEAVRDAGIEATEANAERIG
                     VSIGSGIGGLQLIEDTDKIYRESGPRKISPFFIPGTIINMISGNLSILYGFKGPNIAI
                     VTACTTATHAIGDAARLIEYGDADVMVAGGAEAAITELGVGGFAAARALSTRNDDPAT
                     ASRPWDVDRDGFVIGEGAGVLVLEEYEHAKKRGAKIYCEVAGFGMSADAYHMTSPSEN
                     GEGAARCMRNALRNAGINADEVNYVNAHGTSTPAGDVAETQAAKGFFGDHARKIAMSS
                     TKSMTGHLLGAAGGIEAVFSALAVRDQIAPPTINIFNQDPQCDLDYVANEARSMKIDV
                     AISNSFGFGGTNGTLVFRKV"
     gene            complement(4292..4528)
                     /gene="acpP"
                     /locus_tag="PKW44_05500"
     CDS             complement(4292..4528)
                     /gene="acpP"
                     /locus_tag="PKW44_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HQR51074.1"
                     /translation="MSDIEARVKKIVSEQLGANEADVKNESSFVDDLGADSLDTVELV
                     MALEEEFDCEIPDEDAEKITTVQQAIDYVNAHLK"
     gene            complement(4618..5358)
                     /gene="fabG"
                     /locus_tag="PKW44_05505"
     CDS             complement(4618..5358)
                     /gene="fabG"
                     /locus_tag="PKW44_05505"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HQR51075.1"
                     /translation="MLEGQIALVTGASRGIGAAIARELGQQGAIVIGTATSAGGAEQI
                     GQALQQAGIKGAGMALDVNDAAQVEAVLKEISEKFGVISILVNNAGITRDTLLMRMSE
                     DDWDAVLSTNLKSVFRLSQAVLRPMMKARYGRIISISSVVGHMGNAGQTNYSAAKAGM
                     AGFTKSLAQEVGSRGITVNCVAPGFIDTDMTRDLPEEQRNKLLQHIPLGRLGQVEDIA
                     AAVSFLASARAGYVTGETLHVNGGMYMA"
     gene            complement(5370..6296)
                     /gene="fabD"
                     /locus_tag="PKW44_05510"
     CDS             complement(5370..6296)
                     /gene="fabD"
                     /locus_tag="PKW44_05510"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HQR51076.1"
                     /translation="MKFAFLFPGQGSQSVGMMQGFGDLAVIRDTFVEASDVLGQDFWR
                     MATEPNEELNKTANTQPLMLTAGIATWRAWQQAGGTQPTMMAGHSLGEYTALVAAGAL
                     DFASALPLVRYRAEVMQGAVAEGVGAMAAILGLDDDTVRAVCAESAQGEVLEAVNFNS
                     PGQVVIAGNKAAVERGMEAAKARGAKRALILPVSVPSHCALMKPAAEKLQACLADVAV
                     SVPQVPVLHNADVAAYNEAAQIKEALVRQLYSPVRWVETVQTMAAQGITQAAECGPGK
                     VLAGLNKRIVAEMPCVALIGGDALAETKLQFI"
     gene            complement(6306..>6975)
                     /locus_tag="PKW44_05515"
     CDS             complement(6306..>6975)
                     /locus_tag="PKW44_05515"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HQR51077.1"
                     /translation="QHKLGIAGCPAFDVQAVCSGFVYALTTADQFIKSGMAKCALVVG
                     AETISRITDWTDRSNCILWGDGAGAVILAASDEQGIISTHLHADGSYNDLLHVPRGAT
                     NQDCKPTIQMEGNAVFKMAVNTLDAIVDETLGANGMEKAHVDWLVPHQANIRIIQATA
                     KKLGMSMEQVVVTVGEHGNTSAASIPLALDVAVRDGRIKRGETILMEAFGGGFTWGSV
                     LLKY"
BASE COUNT         1376 a         2110 c         2019 g         1470 t
ORIGIN      
        1 cgcggcggta tcggcatatc caccttgctg caacggctgc gtatggttgc cagcaagcgc
       61 tgcggctgat ggctgactag catgaacagc acgccggagg gcggttcctc caacattttc
      121 agcaaggcgt tggcggaggc ctgattcaat gcttccgctg gatgcagcag gacaatgcgc
      181 aacccggaac gatggctgga cagccccaga aactcagtca gttcccgcac ctggtctacc
      241 gcgatctggg tttttctggt ggttctgggg gcagcacctt catcttcggt gttggcatca
      301 ccctcctctg gttccaccag cctgaaatcc gggtgattgc cctgcgcaaa ccagacgcag
      361 ctctggcagt tgccgcaggc gagttgatcg gctgcagtgt tctcgcacag cagcgattgc
      421 gccagcagtg cggcaaaatc aaatttgccg accccctcac gaccttgtag cagcaaggcg
      481 tgagacagac gctcccgcgt tgcggtcagc ttgccccata acgctttctg ccacggatat
      541 aatttcatga taattatagt gttgaaatta tttcttcaag tcttttatta atattttcca
      601 gggtgtcagc ggaatcaatg atgcgaaatc ttgccgggaa ctgcccggca cgtttgaggt
      661 aagcccggcg tatacgctca aaaaaatcag cctgctcgcg ttcaaaacgg tcagggtcac
      721 gtgtgccggc cagacgctcc aggcttatgg ataccgggac gtcaaacaac agcgtcaaat
      781 ctggctgaaa atcctgttgc acccagcgtt ccaactgggt gatcttgttc tcgcccaagc
      841 cgcgtccgcc actctggtag gcgaaactgg cgtccgtgaa gcgatctgac agcacatcaa
      901 taccgcgcgc cagcgccggc aggatcacct gctccagatg ttcacgccgc gcggcaaaca
      961 tgagcaaggt ctcggtttcc gggtgcatgg ggtggtgtaa cagcagttca cgcagttttt
     1021 ctccgagcgg tgtaccgccg ggttcgcgcg ttaccagcac ttcattgcct cgcgcacgca
     1081 gcaaggcggc gatggccgga atgtgcgtgc tttttcctgc gccgtcgaca ccttccagcg
     1141 taatgaactt gcctgccatc aatctctccg tcttaattgg taacgtgcta ccgcgttatt
     1201 atgctcgact aaggaattgg agaaggcatg gctgccgtca cctcgtccga cgaaatacag
     1261 tgcaccacct cgcgccggat gcagtgccgc ctcgatggcg gcttggccgg gcatggcgat
     1321 aggtgtcggc ggcaggccgt cccgggtgta ggtgttgtaa ggggtgtcgc gcagcagatc
     1381 gcgcttgcgc aggttgccgt caaaagcttc gcccatgcca tagatcacgg tcgggtcggt
     1441 ttgcaggcgc atgccgagtt tgatgcggtt gaggaagacg cgcgcaatca acggccgttc
     1501 accgccgcgg ccggtttctt tttcgatgat ggaagccata atcagtgcct gatagggtgt
     1561 ggcgtacggc aagccggtat ccctgccctg ccaggccagg tcgagtttct gccgcatggt
     1621 ctggtaggcg cgcttgagga tgtcgagatc gctcataccg ctgctaaaat agtagctgtc
     1681 ggggaagaac agaccttccg gcatggtttc ggtcgcaccg atctgctcca tgatctgtgc
     1741 gtctgtcagc ggcatcgaca agtggcgcac cgactcgtgt gcattcaggg cctcacgcat
     1801 ttgcatgaag gtccagcctt cgatgaaggt gatgctgttc tgcactgtgc tgccttcagt
     1861 catcatctgg aacagagcaa acccggtgat gccctgctcc aactggtagt tgccagcctt
     1921 gatctcactt gccttgccca tggcgcgcac cagcagcatg aaactccatg gctctttcag
     1981 cactctctcg ctgaccagtt gctgcgcgat gccgcgcagg ctactgccat gtttcaacac
     2041 catgtcatag ggtgtgcggg gtagttccag gggctgcgaa gcaaaatgat atagccagcc
     2101 gccggccagc aacagcaggc cgaacaggcc tgataccagc agtctacgca gtaggcgcat
     2161 ggtcttcctt caacagcgtt cgtagccggc tcgctaattc gcccgcgggc caggttttcc
     2221 cggccagttc acgcacctgc caggcaccga acaggctgtt gcacagcacg acttcatccg
     2281 cgctcagcaa gctgtccagc ttgaagctgc ataccctgac ctcgaaacca agcgtcgtcg
     2341 ccaactccac gatacgctgg cgggtcacgc cggcaacgcc gcattgcgtg aggtcgggcg
     2401 tcaacaggat atcgttctgg cggatgaaga cattgctcat ggtgcactcg atgacattgc
     2461 cggcgatatc gagcaacagg ccgtcggcaa tgttcgcatc cgtccattcc atacgcgcca
     2521 gcacgttttc cagtcgattg aggtgcttga ttcccgccag ccgaggttgg gaggaaagcg
     2581 ccagttggca aagatgaagg gaaacgccat aggaaaaatt ggattccggc cagatcggca
     2641 gcccggattt gagcagcacc cggttgggtt gtgccagtgg tggcactgca tagccgcgca
     2701 tgccatttcc ccgcgtgacg attattttga aggccgcatc ctcgtcatcg ccgcatagcc
     2761 gggtaatgtc gccgagcagc aattctgcag gcggacagac gatgccaagc gcattgcagt
     2821 cgtcactcaa acggtgatag tggcgctccc agcaatgcgg atgcccccga tataccggca
     2881 aggtgcgaaa aaccccatcg ccataggcaa agccacggtc caagggcgtc aggccgtcgt
     2941 gtcggccgtt aatcaggaac tggttctgca tgaggctgcc atcctagcgc ataggcgccc
     3001 cgtcgcaaag caattgtcgg tctgctaaac cttgcggaac acgagggtgc cgttggtgcc
     3061 gccaaacccg aaggaattgg aaatggcgac gtcgatcttc atgctgcgcg cttcgttggc
     3121 cacgtagtca agatcgcact gcggatcctg gttgaagatg ttgatggtag gtggcgcaat
     3181 ctggtcgcgc actgccagtg cggaaaatac cgcctcgata ccgcctgcag cacccagcag
     3241 gtggccggtc atggatttgg tggaactcat ggcgattttt cgcgcatggt cgccgaagaa
     3301 gcccttggcc gcctgagttt ctgccacgtc gccagccggc gtggaagtgc cgtgggcatt
     3361 gacgtagtta acctcatcgg cgttgatgcc ggcgttgcgc aaggcgttgc gcatgcagcg
     3421 cgcggcacct tcgccgtttt cacttggtga cgtcatgtgg tatgcatcgg cactcatgcc
     3481 gaaaccggcg acctcacagt aaattttggc gccgcgtttt ttggcatgtt cgtattcttc
     3541 cagcaccagc acgccggcac cttcgccgat gacgaagcca tcgcgatcca cgtcccacgg
     3601 gcggctggca gtggccgggt cgtcattgcg ggtagaaagg gcgcgcgccg cggcaaatcc
     3661 ccccacccca agctcggtaa tggcggcctc ggcacccccc gccaccatca cgtcggcatc
     3721 gccatactcg atcaagcgtg ccgcatcacc aatggcatgg gtagccgtgg tacaggcggt
     3781 gacaatggcg atgttgggcc ccttgaaccc gtacaggatc gacaggttgc ccgaaatcat
     3841 gttgatgatg gtgcccggaa tgaagaacgg cgaaatcttg cgcggaccgg attcccggta
     3901 gatcttgtcg gtatcctcga tcagttgcag gccgccgatg ccggagccga tactgacgcc
     3961 gatgcgttcg gcattggctt cagtggcttc gatcccggca tctctgacag cctcgattcc
     4021 cgccgccagg ccgtactgga tgaaagtatc catgcggcgc gcatccttgg cagggaggta
     4081 ctggctgaca tcgaaccctt tcacctcgcc agcgacctga gacgaaaagg tgctcggatc
     4141 gaacttggtg atgcgagtga tgccggattt accggcgatg atgtcggccc aggcgttcgc
     4201 cacaccgata cctaccggcg aaacgatacc taggccggta acgactaccc tacgtttgga
     4261 caaggcggct ccggggaaaa ttgaggttgg attacttcag gtgcgcgttg acgtagtcga
     4321 tcgcttgctg gacggtagtg atcttctcgg catcttcgtc cggaatttcg cagtcgaact
     4381 cttcttccag cgccattacc agttcaacag tgtcgagaga atcggcaccc aggtcatcca
     4441 cgaaggaaga ttcgttcttg acgtcggctt cattggcgcc cagttgctcg gaaacaatct
     4501 tcttgacgcg cgcttcgata tcggacattt ggttcccctt taaagtaagt tacagcattt
     4561 gagtacagca aaaactcggg ctattttagc aaaagtcccg aggaattcaa aaaggcatta
     4621 cgccatgtac atgccaccgt tgacatgcag cgtctcgccg gtgacatagc cggctcgtgc
     4681 cgatgccagg aaagacaccg ccgcggcaat gtcttccacc tgccccagcc gccccagcgg
     4741 aatgtgctgc agcaacttgt tgcgctgttc ttccggcagg tcgcgcgtca tgtcggtgtc
     4801 gatgaagcca ggcgccacgc agttgacggt gatgccgcgg ctgccgacct cctgcgccag
     4861 cgacttggtg aagcccgcca tgccggcctt ggcggcggaa taattggtct ggccggcatt
     4921 gcccatgtgg ccaaccaccg aggaaatact gatgatgcgg ccgtagcgcg ctttcatcat
     4981 cggacgcagc acggcctgcg acaggcggaa taccgacttg agattggtgg acagcaccgc
     5041 atcccagtcg tcctcgctca tgcgcatcaa cagggtgtcg cgtgtgatgc cggcattgtt
     5101 gaccagaatg gagatcacac cgaatttttc tgaaatttcc ttgagtacgg cctcgacctg
     5161 cgcagcatca ttgacatcca gcgccatgcc ggcacccttg atgccggcct gctgcaaagc
     5221 ctggccaatc tgttccgcgc cgcccgcact ggtggcggtg ccgatcacga tagcgccctg
     5281 ctgacctagt tcccgggcaa tagcggcgcc gatgccacgg ctggcgcctg tcaccagcgc
     5341 aatctgtcct tcgagcatgt tcattcccct caaataaatt ggagtttggt ttcggccaag
     5401 gcgtcgccgc cgatcagcgc cacgcagggc atttccgcca caatgcgctt gttgaggcct
     5461 gccagcacct tgccggggcc gcattctgcc gcctgggtga tgccttgtgc cgccatcgtc
     5521 tggaccgtct cgacccagcg caccggactg taaagctggc gcaccagggc ttctttgatc
     5581 tgtgcggcct cgttataagc cgccacgtcg gcattatgca gaaccggaac ctgcggtacc
     5641 gaaacggcga catctgccag gcaggcttgc agtttttctg ccgccggctt catcagcgcg
     5701 caatgcgacg gtacactgac cggcaggatc agcgcgcgtt tggcgcccct ggccttggcg
     5761 gcttccatgc cgcgctccac ggctgccttg ttgccggcaa tgactacctg ccccggcgaa
     5821 ttgaaattga cggcttccag cacttcgcct tgggcggatt ctgcacatac cgcgcgcacc
     5881 gtgtcgtcgt ccagaccaag aatcgccgcc atagccccta ccccctcggc aaccgctccc
     5941 tgcatcacct cggcacgata gcgaaccaga ggcagagccg aagcgaaatc cagtgcgccg
     6001 gcagcaacca gtgcggtata ttcgcccagg ctgtggccgg ccatcatggt cggctgtgtg
     6061 ccacccgcct gctgccaggc gcgccatgtg gcgataccgg ctgtcagcat caaaggctgg
     6121 gtattggcgg tcttgttcag ttcttcgttg ggctcggtcg ccatgcgcca aaaatcctga
     6181 ccgagtacgt cggaggcctc gacaaaagta tcacggatga cggccaagtc gccgaaaccc
     6241 tgcatcatgc cgaccgactg ggaaccctgt cccggaaaaa gaaaagcaaa tttcatcgtc
     6301 atatcctagt acttgagcaa taccgaaccc caggtgaacc cgccaccgaa agcttccatc
     6361 aggatcgttt ctccgcgctt gatccggccg tcacgcacgg cgacgtccag tgccagcgga
     6421 attgacgctg ctgaagtgtt gccgtgttca cccacggtga ccaccacctg ctccatgctc
     6481 atgccgagtt tcttggccgt ggcctggata atgcggatgt tggcctgatg tggcaccagc
     6541 cagtctacat gcgccttttc catgccattg gcgcccaggg tctcatcgac gatggcgtcc
     6601 agcgtattga ccgccatctt gaatacggcg ttgccttcca tctggatggt aggcttgcag
     6661 tcctggtttg tggcgccacg cggaacgtgc agcaggtcgt tgtagctgcc atcggcatgc
     6721 aggtgggtgg aaatgatacc ctgttcgtca ctggcggcaa gaatcacagc gccggcgccg
     6781 tcgccccaca ggatgcagtt gctgcgatcc gtccagtcag taatgcgcga aatggtctcg
     6841 gcgcccacga ccagcgcaca ttttgccata ccgctcttga tgaactggtc ggcggtggtc
     6901 agcgcataga caaagccgct gcacacagcc tgcacgtcaa acgcggggca gcctgcgatg
     6961 ccgagcttgt gctgc
//
LOCUS       DAOMFA010000085         6864 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_1380_length_6864_cov_4.369070, whole genome shotgun sequence.
ACCESSION   DAOMFA010000085 DAOMFA010000000
VERSION     DAOMFA010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 6864)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6864)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6864
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1380_length_6864_cov_4.369070"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1140
                     /locus_tag="PKW44_05520"
     CDS             <1..1140
                     /locus_tag="PKW44_05520"
                     /inference="COORDINATES: protein motif:HMM:NF016086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HQR51078.1"
                     /translation="DADSDANSDINPDIHCHVELDADIDADIHLDFHRYADSHGNHNN
                     ALPSRAADNIYWVGRYAERAEGSIRLLRATVKKLYINPDRGNPDYEGSLHNLLRGVTN
                     ITGTWPGFFSEQPEALLQSPEPELLSVALDEQRAGSIAHSLRCLVQAGYAVRDLWSSD
                     TWRVMDEMEEHLTLSQQLGDSTLWQMQEHMDRLITTLCAFSGLVMENMTRGNGWLFLD
                     IGRRLERSLLLISTLRTAFSVRQSDGMETQLIESLLDTSDNTICYRQHYRSYFELPSF
                     LELLLLDTCNPRSLAYQIANLNEHVTKLPGSPHRQRLSTEQRLVLEASSMLNLTHLDD
                     LLVVSKDGVRENLDQQLSRLYYLLGTLSEAVTADYFRHGNAPQSL"
     gene            1158..2039
                     /locus_tag="PKW44_05525"
     CDS             1158..2039
                     /locus_tag="PKW44_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013146735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HQR51079.1"
                     /translation="MRYLVTHRTEYEYVETVGHSYNEVRLLPRFCEHQNLISARLEIT
                     PGEADYRVRTDFFGNQVAYFSIPEPHREFSVTAVSEVQIFNQPGQLDFAQGASWESVK
                     QTLRLNRLPEVLHHHQYTLDSPFIAAGAELAAYANPSFPPGRPLVEAVHDLMQCIHRD
                     FIFDPEFTTLATPLAAVLEHQRGVCQDFAHLAIDCIRSHGLAARYVSGYIETLPPPGK
                     QKLVGADASHAWFSVYLPDIGWMTFAPTNNQLPGDQHITVAWGRDYGDVTPLKGVIFG
                     GQNHELKVSVEVERLGT"
     gene            complement(2188..2379)
                     /locus_tag="PKW44_05530"
     CDS             complement(2188..2379)
                     /locus_tag="PKW44_05530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR51080.1"
                     /translation="MGLVGSQFDIDLVFAIGAAVGLAFFYQFLPPRLQVGHFVRVLSC
                     GERRRGKAGKRQQHRGDGK"
     gene            complement(2480..3685)
                     /locus_tag="PKW44_05535"
     CDS             complement(2480..3685)
                     /locus_tag="PKW44_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HQR51081.1"
                     /translation="MDSLKSRALEYHEYPSPGKLSVESSKPCATQDDLSLAYTPGVAA
                     PVREIHRDPEQAYRYTNKGNLVAVITDGTAVLGLGNVGALAGKPVMEGKAVLFKKFAG
                     IDVFDIEINAPSVEDFIETVVNIAPTFGGINLEDIAAPHCFRIEKELKARLDIPVFHD
                     DQHGTAVIISAALLNALELQGKSLATAKIVFLGAGAAGAATARLLRVMGAKRITMVDK
                     DGVLHKGRPDLDKLPADLAITTAARTLADVMPGADVFIGVSAANALPAALLKRMADRP
                     VVFALANPDPEILPSVARSVRDDLIMATGRSDFPNQVNNALCFPYLFRGALDARAKNI
                     SVEMQIAAAMALADLAREPVPDEVLKAYNLTELCFGKDYIIPKPFDPRLIERIPPRVA
                     EAAKRQANV"
     gene            complement(3780..4226)
                     /locus_tag="PKW44_05540"
     CDS             complement(3780..4226)
                     /locus_tag="PKW44_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2721 domain-containing protein"
                     /protein_id="HQR51082.1"
                     /translation="MLSTPSDIHVSFAIRDAVAPVFLLTGIGSILSVLVNRLGRSIDR
                     ARTLNALKPEQRQAFLDEFDIIVRRTRWMRWSVGLFIFAGLCVALSIASVFIGVAVGV
                     HLTSFVLFTFITAMFSLIIGLLCFLREIVLASQEVITPVRQDLNAQ"
     gene            complement(4230..6864)
                     /locus_tag="PKW44_05545"
                     /pseudo
     CDS             complement(4230..6864)
                     /locus_tag="PKW44_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829276.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transglutaminase family protein"
BASE COUNT         1366 a         2129 c         2047 g         1322 t
ORIGIN      
        1 gacgcagaca gcgacgcgaa ctccgacatc aaccccgaca tccactgcca cgtcgagctc
       61 gacgccgaca tcgacgccga catccacctc gacttccacc gttacgccga cagccacggc
      121 aaccacaaca acgcgctgcc cagccgcgct gcggacaaca tttactgggt cggacgctac
      181 gccgagcgcg ccgagggcag catccgtcta ttgcgcgcca ccgtcaaaaa actttatatc
      241 aaccccgacc gcggcaaccc ggattacgaa ggtagtctgc acaatctgct gcgcggggtg
      301 accaacatca ccggcacctg gcccggcttt ttctcggaac agccggaagc gttgctgcag
      361 tcgccggagc cggaactgct gtcagtcgcc ctggacgagc agcgcgccgg cagcatcgcc
      421 cactccctgc gttgtctggt gcaggccggc tacgcagtgc gcgacctgtg gtcgtcagac
      481 acctggcgcg tgatggacga gatggaagaa catctgaccc tctcccaaca gctcggcgac
      541 agcaccctgt ggcagatgca ggaacacatg gacaggctca tcaccaccct gtgcgccttc
      601 agcggcctgg taatggaaaa tatgacgcgc ggcaacggct ggctgttcct tgatatcggc
      661 cgccggctgg aacgcagcct gctgctgatc tcgacgctgc gcaccgcgtt ttcggtgcgc
      721 cagagcgatg gcatggaaac gcagctgatc gaatcgctgc tggataccag cgacaacacc
      781 atctgctacc gccagcacta ccgcagctat ttcgagctgc cttcgttcct ggaattgctg
      841 ctgctggata cctgcaatcc gcgctcgctg gcctaccaga tcgccaacct gaacgagcat
      901 gtcaccaaac tgccaggcag tcctcatcgc caacgcctca gcacagagca gcgtctggta
      961 ctggaagcca gctccatgct caacctgact cacctggacg acctgctggt cgtgtcgaag
     1021 gacggtgtac gcgagaacct ggaccagcag ttgagccgat tgtattacct gctcggtacg
     1081 ctgtcggaag ccgttaccgc cgattatttc cgccacggca atgcgccgca atcattgtag
     1141 atgtagaggc tgattcaatg cgctacctcg tcacgcacag aacagaatac gaatacgttg
     1201 aaaccgtcgg acactcctac aacgaagtcc gcctgctgcc gcgcttctgc gaacaccaga
     1261 acctgatctc ggcacggctg gaaatcacgc cgggcgaggc tgactaccgc gtccgcacgg
     1321 atttcttcgg caaccaggtc gcctattttt ccatcccgga accacaccgc gagttcagcg
     1381 tcaccgccgt cagcgaagtg cagattttca accagcccgg acagctcgat ttcgcccagg
     1441 gggcctcatg ggagtcggtc aagcagacac tgcggctgaa ccgcctgcca gaagtgctcc
     1501 accaccacca gtacactctg gattccccct tcatcgccgc cggcgccgag ctggcggcct
     1561 atgccaaccc ttcgttcccg ccgggccggc cgctggtcga ggcggtacac gacctgatgc
     1621 agtgcatcca ccgcgacttc atcttcgacc ccgaattcac caccctcgcc accccgctcg
     1681 ccgccgtact ggagcaccag cgcggtgtat gtcaggactt cgcccacctt gccatcgatt
     1741 gcatccgctc gcatggcctg gctgcccgct acgtcagtgg ctatatcgaa accctgccgc
     1801 cgccaggcaa gcagaagctg gtcggcgccg atgcctcaca tgcctggttc tcggtctatt
     1861 tgccagacat cggctggatg accttcgccc ccaccaacaa ccaacttccc ggcgaccagc
     1921 acatcaccgt cgcctggggc cgcgactacg gtgacgtgac accgctgaaa ggcgtcattt
     1981 tcggcgggca gaaccacgag ttgaaagtgt cagtggaagt ggagcgactg ggtacataaa
     2041 acgcttgcat tgtgatccag gcaggtcaac aatcaaacac actctggcgt tgccgggtgt
     2101 cgtttgatct gttgcgtttg gcggtggaaa actggctgaa cactctctgc ggaaaggctc
     2161 atcatgaaac ctgcccacca aaatcttcta tttcccatcc ccgcggtgtt gttgacgctt
     2221 gcctgccttg ccgcgccgtc tttcgccgca gctcaagact ctgacgaaat ggccaacctg
     2281 caggcgcggc ggcaggaatt gataaaaaaa tgcgaggcca accgcggcac cgattgcgaa
     2341 cacgaggtcg atgtcgaact gggagccgac cagacccaca gtcccagtgt gtacatcccg
     2401 ccggaatatg atagaacccg gccaatccgg ccggttccca gacctgcccc ccgcagcaga
     2461 tccagatgag ctggatgcgt cagacgtttg cttgacgctt cgccgcctcg gcgacgcggg
     2521 gcgggatacg ctctatcagc cgtgggtcga agggtttggg aataatgtaa tccttgccga
     2581 agcacagctc ggtcaggttg tacgccttta gcacctcgtc aggaaccggt tcgcgcgcca
     2641 ggtctgccaa cgccattgcc gcggcaatct gcatctccac tgagatgttt ttcgcgcgtg
     2701 catcgagagc gccacggaac aggtaaggga aacatagggc attgttgacc tgattgggga
     2761 agtccgaacg tccggtagcc atgatcaggt catcccgtac tgacctggcc acagaaggca
     2821 gaatttccgg gtctggattc gctaaagcga acactacagg tctgtctgcc atgcgcttga
     2881 gcaacgccgc gggcaatgca ttggcagcag aaacaccgat gaacacgtcg gcacccggca
     2941 tgacatcggc cagcgtgcgc gcggcggtag tgatggcgag atcggcaggc agcttgtcca
     3001 gatcggggcg acctttatgc aacacgccat ccttgtccac catggtgatg cgcttcgcgc
     3061 ccatgaccct cagtagtctc gcggtggcgg cacccgctgc gcctgcaccg agaaaaacga
     3121 tttttgcggt cgccagtgat ttcccctgca actccagcgc gttcagcagt gctgcgctga
     3181 tgatcacggc ggtgccgtgc tggtcgtcgt ggaataccgg tatatccagc cgcgccttca
     3241 attccttctc gatgcggaag caatgcggcg ccgcaatgtc ttcaaggttg atgccaccga
     3301 aggtcggggc gatattgacc acggtttcga tgaagtcctc gacgctggga gcattgatct
     3361 cgatatcgaa gacatcgatg ccggcaaatt tcttgaacag taccgctttg ccttccatca
     3421 ccggcttgcc ggcaagggca ccgacattac ccagccccag cacggcggtg ccatcggtga
     3481 tgaccgccac caggttacct ttgttggtgt agcgatatgc ctgctcggga tcgcggtgga
     3541 tctcccgcac cggggcggcg acacccggcg tataggccaa ggacaggtca tcctgcgtgg
     3601 cacagggttt ggaggattcg accgagagct tacccggaga ggggtactcg tgatattcca
     3661 gagcgcgtga cttgagagag tccatgaggg gtgtgctctt aaggtgaatg gatggaaact
     3721 acattatcga taactggtac acgaatgatg tcagctttaa ctgtgaaacg ccatgcgatt
     3781 cattgcgcat tcaaatcttg tcgaacaggc gtaatcactt cttgtgatgc cagaacaatc
     3841 tctctaagaa agcagagcag gccaataatc agtgaaaaca tcgccgtgat aaaggtgaat
     3901 aaaacgaagc tcgtcaaatg cacgccgacg gccaccccga tgaataccga tgcaatcgac
     3961 aaagccacgc atagcccggc aaagatgaac aggccgaccg accatcgcat ccatcgcgtt
     4021 cttctgacaa tgatatcgaa ttcatccagg aacgcttgtc gctgctcagg ctttaaggca
     4081 ttcaaagttc tggcacgatc gatggacctc cccaaccgat tgacaagcac cgagagaatc
     4141 gagccgattc cggtcagtaa aaaaaccggg gcaaccgcat cacgtatagc aaaagagaca
     4201 tggatatctg atggcgtgct cagcatcttt tatcgggcgt tccagcgcaa atcgagcgta
     4261 aacggagtgt ccttgttggg ctcttccggc ggcaggtcca cggcgctgtc gcccggcgtg
     4321 tggccgtggt cccagaagcg gctgacacgg cggccttcgg cctcgtaggc gttgaccggc
     4381 aggctgtcgt agttgcggcc gccggggtgg ctgacgtggt aagtgcagcc gccaatggcg
     4441 cggctgttcc aggtatcaac gatgtcgaac accagcgggg cttgcacgcc tatggtggga
     4501 tgcagcgcgg aaggtggatt ccaggcgcgg taacggatgc cggcgaccat ttcgccgggc
     4561 gtggcggtcg cccgcagcgg gacgcggcgg ccgttgcagg tcagggcata gcgttcaccc
     4621 atcgcgccgg tgagcttgac ttgcaggcgc tccaccgacg aatccacata gcgtgccgtg
     4681 ccgctgctgg taatttcctc gcccagtaca tgccagggtt cgatggcctg gtgcagttcc
     4741 agtttgaggt cgccgacttg cagcgcgccc atgtgcggga agcggaattc caggaacggc
     4801 gcgaaccatt cggccttgaa ggcatagcca gcgctttgca ggtcgcgcgc cacgtcatgg
     4861 atgtcggact caatgaaatg cggcagcata aagcggtcat gcagttgcgt gccccagcgc
     4921 accagcggct tcttgtatgg ctgcttccag aaccaggcga ccagcgtgcg cagcagcagc
     4981 atctgcatca gcgacatctg cgcgtgcggc ggcatctcga aggcgcgcag ttccagcaac
     5041 cccagccgcc cggcatccgt gccgggcgcg tagagcttgt cgatgcagaa ttcgctgcgg
     5101 tgggtgttgc cggtcatatc caccagcagg ttgcgcagca gccggtccac cagccagggg
     5161 gcggggactt cgccgtccgg catctgctgg aaggcgattt cgagctcgta caggctgtcg
     5221 ttgcgcgctt cgtccacgcg cggagcctgg ctggtcgggc cgatgaacag gccggagaac
     5281 aggtaggaca gcgccgggtg gtgctgccag taggtcacca ggctgcgcag cagatcggga
     5341 cggcgcagga tgggactgtc ggccggcgtg atgccgccca gggtgacgtg gttgccgccg
     5401 ccggtgccgg tgtggcgtcc gtccagcatg aatttctcgg tacctagcct ggtcagccgc
     5461 gcctgctcat acagcgtcgt ggtgttgtgc accagttcct tccaactggc cgccggatgg
     5521 atgttgacct cgatcacgcc ggggtcgggc gtcaccggca ggcgcgtcaa tcgcagatcg
     5581 tgcggcggct ggtagccctc gatcacgact ggcatcttga gcagcttagc ggtagcctcg
     5641 accgaagcga tgaggtcgag gtaatgctcc agcgcgttgg tgggtgggac gaaaatgtgc
     5701 aggcgtccct cgcgcgcttc caccgtcaat gcagtatgcg gcgtccactt atgttcatcc
     5761 agagtttcgt cgtcggccgc ctccatctcg gtggcatgcg tagctttttt gctggccttg
     5821 ctatatctgc ggctgaccag ggaatcgatg tcacccagcg catcggcctg ttcatacagg
     5881 ctgcgttcgg gttcatggtc acggtcttcg aacggcaccc agggcaggct gttgagcggc
     5941 aggcgcaggc ccatggcgga atcgcccggc accaggaaca tctcgccgcg gcggaattcc
     6001 cacgggctgc tgctccaggt attgatcttg tggctccagt gcagcggtag cacgtaaccc
     6061 accggcgcgc cgaggtcacc ggccagcacc cgggcgaggc gcttgcgctc gagatcgtcc
     6121 ttcaaatcca ccttgagtgg gtcgaggttc tgcggcagga caccttcctg caacaggtaa
     6181 tagagcggat cctcaaagcc ggggcggata tagctgccct tcaggccgag gcgtttcgcc
     6241 agctcccgga tgaacttgtc ggcatgtgga cgcttgaagc cgtagtcttt ggtttcctcg
     6301 gccagcaggt cggggttctg ccatatcggt ttgccgtcct tgcgccagaa gcaggccagc
     6361 tgccaacgcg gcagcggctc gcccggatac cacttgccct ggccgaaata tagcaagccg
     6421 ccgggggcga aattgtcgcg cagccgtcgc accagctctc cggccagttt gcgcttctgt
     6481 tcgccgtcag catcggtatt ccactccggc gcgtccatgc tgtcgatcga aacgaaggtc
     6541 ggctcaccac ccatggtgag acgcacgtcg cccttcttca gcgccttgtc caccttgtcg
     6601 cccagtttct cgatcgccgc ccattgctgc tcgctgtagg gcttggtgac gcgcgggtcc
     6661 tcgtggatgc gggtcacgtg gttgccgaag ctgaaggtag tattggcctg gccgacaaag
     6721 ccgccagtaa ccggcgcggc gctgacgtaa tcggcggtgc aggcgagcgg gatatgatct
     6781 tcgccagaga acagcacgga agtcgggtcg aggccgatac agcaagcgcc gggaatgtag
     6841 acttagaacc tggcgtgcag gtcg
//
LOCUS       DAOMFA010000086         6818 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_1396_length_6818_cov_4.719799, whole genome shotgun sequence.
ACCESSION   DAOMFA010000086 DAOMFA010000000
VERSION     DAOMFA010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 6818)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6818)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6818
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1396_length_6818_cov_4.719799"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(248..1780)
                     /locus_tag="PKW44_05550"
     CDS             complement(248..1780)
                     /locus_tag="PKW44_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome D1 domain-containing protein"
                     /protein_id="HQR51083.1"
                     /translation="MRILLAFLAVLVCHNGLADAQQLFQQHCAGCHGSDRLGGAGPAL
                     LPESLERLKKPEALKTIAEGRIATRMAGFADKLSVDELQALVDYVYTPLPQAPVWGDA
                     EIRSSRIEIHKPGSLPDKPAFHADMLNLFIVVETGDHHATLLNGDSFEPIRRFPTRFA
                     LHGGPKFSPEGRYVYFASRDGWVSKFDIWNLEYVAEIRVGINTRNIAVSGDGRYVMAA
                     NYLPHTLAVLDAADLSLIKTIPAQDENGHSSRVSAVYDAAPRKSFVVALKDIPELWEV
                     SYDDNAKPIYPGYVHDYKMAEAIAIPGKLNPRRTPLETVLDDFFFDQSYAYLIGASRE
                     GKGQVVNLDVRRKVADLPLPGMPHLGSGITWEWQGRPVLATHNLKDGVVTVIDMQNWQ
                     VVKQIKTPGPGFFMRSHENTRYAWTDVFNGATKDTLQLIDKQTLEIAATITPVPGKTA
                     AHVEFTRDGRYALVSVWEDEGALVVYDAETLREIKRMPMRRPSGKYNVYNKITRSTGT
                     SH"
     gene            complement(1780..2982)
                     /gene="nirJ"
                     /locus_tag="PKW44_05555"
     CDS             complement(1780..2982)
                     /gene="nirJ"
                     /locus_tag="PKW44_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011310584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme d1 biosynthesis radical SAM protein NirJ"
                     /protein_id="HQR51084.1"
                     /translation="MFRISQYMQEIADPKPLGPRRNPPGPVVIWNLVRRCNLTCQHCY
                     SISADKDFPGELSTEEVFKVMDDLKAFRVPVLILSGGEPLLRPDIYDIARRAKAMGFY
                     VGLSSNGTLITEANIGVIAGIGFDYVGVSLDGIEATHDKFRRKPGAFKESLHGIRLCR
                     DAGIKVGVRFTLTQDNAHDLPALLQLVKDEGIDKFYLSHLNYAGRGNKNRGHDAFLET
                     TRKAMDLLFDTCWNDTKQGRHREFVTGNNDADGVYLLFWVRKNFPHLEPHMRAKLAQW
                     GGNSSGVNVANIDNLGVVHPDTFWWHYPLGNVRERPFSEIWQDTSDPLMAGLKATPRT
                     IKGRCGECSCFDICGGNTRVRAQQLTGDAWQEDPACYLSDEEIGVNSDRERIAVTPYR
                     KITLQQVA"
     gene            complement(2982..3461)
                     /locus_tag="PKW44_05560"
     CDS             complement(2982..3461)
                     /locus_tag="PKW44_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AsnC family transcriptional regulator"
                     /protein_id="HQR51085.1"
                     /translation="MSLKLDVVDRAIVQATQSGLPLTARPYHDIAEQLGVPPEVVMRR
                     MQAMLDDGVIRRIGAVPNHYALGWSANGMTVWDVINDQVDELGKQVGALEFVSHCYRR
                     PRRLPDWRFNLFAMVHGRDRAEVEAKARQIAALLGTNCRAHSILYSTKILKKSGLRI"
     gene            complement(3458..3901)
                     /locus_tag="PKW44_05565"
     CDS             complement(3458..3901)
                     /locus_tag="PKW44_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AsnC family transcriptional regulator"
                     /protein_id="HQR51086.1"
                     /translation="MDATDRDIVNGLQGGFPICERPYREMAQQFGISEEELIARLENL
                     LAEKVLTRFGPMFQIERMGGAFSLAAMKVPEEDYDRVTGIVNALPEVAHNYRREHEFN
                     MWFVLATETPEGISQAIAKIETESGLAVYNMPKETEYFVGARFTA"
     gene            complement(3888..4874)
                     /locus_tag="PKW44_05570"
     CDS             complement(3888..4874)
                     /locus_tag="PKW44_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HQR51087.1"
                     /translation="MNGFNLIEQRLLNEYQHDFPLVKRPFLAIGDQLGIEEDEVIERL
                     GQLSARGAVSRVGPVFSPGCIGASTLAALAVPPGKLDEMAHWINALPEVNHNYEREHN
                     FNLWFVAAAPGQAWLDSVLKKIASESGCPMLVLPLVERFHIDLGFDLNGRSCPSRRSS
                     GSSTRVQLDSAQTAIVAGLQSGIKLERQPFAAIGERVNLDEEDVIRQVSAWRGEGIIK
                     RFGVVVRHHELGYRENAMAVWDVPDGQVSALGRLAAQQEGVTLCYRRPRQLPDWPFNL
                     FCMVHGTDRDVVRRLIETLAANTGLDAFQTQVLFSRRRFKQCGARYVADGRN"
     gene            complement(4877..6043)
                     /locus_tag="PKW44_05575"
     CDS             complement(4877..6043)
                     /locus_tag="PKW44_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome D1 domain-containing protein"
                     /protein_id="HQR51088.1"
                     /translation="MCNKWCLAILLLALVGCAGAPVRGTGDLGVVIERDVGRVQVVDT
                     SARKSLATITGLGDLSHASVVFSRDGRYAYVFGRDGGLTKVDLLKRDIDKRILQAGNS
                     IGGAISQDGRYVAAQNYTPGGVKVFDAATLDLVADIPAVYGDGQLSKVVGLTDAPGNR
                     FVFRLFDAGEIWVADISDPSSPEIQKFAAGKQPYDGLITPDGRYYIAGLFGEDGLALL
                     DLWHPEQGTRRILDGYGHGEEKLPVFKMPHLRGWAVAGTRAYLPAIGRHELLVVDTQS
                     WQEAGRIAVAGQPVFAIARPDGRQVWVSFAFPDNGQVQVIDTESQQVRQTLQPGRAVL
                     HFEFTPRGEAAWVSSRDDNKVVIYDTGTLEQVAELPASSPSGIFFTYRSGKIGF"
     gene            complement(6033..6386)
                     /locus_tag="PKW44_05580"
     CDS             complement(6033..6386)
                     /locus_tag="PKW44_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018076712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HQR51089.1"
                     /translation="MSVREISRGLGPAFFLGWIAFFSVGADAGEEVNQQRQRELVRLV
                     RNDCGSCHGLKLGGGLGPSLLPAALAGKAPGALRETIQQGRAGTAMPPWSRFLSEAEA
                     NWIVEQLMKGFPDVQ"
     gene            complement(6403..>6818)
                     /locus_tag="PKW44_05585"
     CDS             complement(6403..>6818)
                     /locus_tag="PKW44_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome D1 domain-containing protein"
                     /protein_id="HQR51090.1"
                     /translation="DQAWKVVRTLNGQGGGSLFIKTHPKSKNLWVDTPLNPEAKVSQS
                     VAVYDIDNLDKGFTMLPIGEWAGLGEGAKRIVQPEYNKAGDEVWFSVWSAKDKESAIV
                     VVDDKTRTLKAVIKDPKLITPTGKFNVYNTQHDVY"
BASE COUNT         1303 a         2052 c         2009 g         1454 t
ORIGIN      
        1 gagactgcag cggtgatgtt atgactattg tcgcgttttg gaagcttctg cgtggtgtcg
       61 aatgtaagcg caagaaaaag taggaaaaaa aacaacgacc caccgaaaaa gatgttgcgt
      121 gccatggatg ttgtaaagtt tttagccatc gcaatccccc tttaatttga gttaatcacc
      181 aagcgtacac tctacgccga accaaaaagt cgaaccttga tatgtatcaa gtttacatac
      241 ctgcatgtca gtggctggtg ccagtcgaac gtgtaatctt gttatacaca ttgtatttac
      301 ctgacggcct ccgcattggc atccgtttga tttcacgcag cgtttccgca tcgtacacca
      361 ccagtgcgcc ctcgtcttcc cacacactga ccagcgcgta ccggccatcg cgggtaaatt
      421 cgacgtgtgc cgcagtcttg ccaggcaccg gggtgattgt ggcggcgatt tccagcgtct
      481 gcttgtcgat cagttgcagg gtgtccttgg tcgctccgtt gaatacgtct gtccaggcgt
      541 agcgggtgtt ctcgtggctg cgcatgaaga agccggggcc gggagtcttg atctgcttca
      601 ccacctgcca gttctgcatg tcgatcaccg tcaccacgcc gtccttgagg ttgtgcgtgg
      661 ccagcactgg cctgccctgc cattcccagg tgatgccgga accgaggtgc ggcatgccgg
      721 gcagcggcag gtcggccacc ttgcgccgga cgtcgaggtt gaccacctgc cccttgcctt
      781 cgcgcgaggc gccgatcagg taggcgtagc tctggtcaaa gaagaaatcg tcgagtacgg
      841 tttccagcgg cgtgcggcgc gggttgagct tgccgggaat ggcgatggct tcggccatct
      901 tgtaatcgtg cacatacccc ggataaatgg gcttggcgtt gtcgtcatag ctcacttccc
      961 acagttccgg gatgtctttc agcgccacca cgaagctctt gcgcggcgcg gcgtcataca
     1021 ccgcggagac gcgcgagctg tggccattct cgtcctgcgc ggggatggtc ttaatgagcg
     1081 agagatcggc ggcatcgagc accgccagcg tgtgcggcag gtagttggcg gccatcacgt
     1141 agcgcccgtc gccggaaacg gcgatattgc gcgtgttgat accgacgcgg atttcggcga
     1201 catattccag gttccagata tcgaacttgg aaacccagcc gtcgcgcgag gcgaaataga
     1261 cgtagcgccc ttccggcgag aacttgggtc cgccgtgcag ggcgaagcgc gtggggaagc
     1321 gcctgatcgg ctcgaaactg tcgccattga gcagcgtggc gtgatggtcg ccggtttcca
     1381 ccacgatgaa caggttgagc atgtcggcat ggaaggccgg cttgtcgggc aggctgccgg
     1441 gcttgtggat ttcgatgcgg gaggagcgga tttcggcatc gccccatacc ggcgcctgcg
     1501 gcagcggcgt gtagacgtaa tcgaccagtg cctgtaactc atcgaccgac agcttgtcgg
     1561 cgaacccggc catgcgcgtg gcgatacggc cctcggcgat ggtcttgagc gcctcgggct
     1621 tcttcagccg ctccaggctt tccggcagca gcgccggccc ggctcctcct aatctgtcgc
     1681 taccgtggca gccggcacaa tgctgctgga acagttgctg cgcatcagca aggccgttgt
     1741 gacagaccag tacggccaga aaggccagca ggatgcgcat cacgcgacct gttgcagcgt
     1801 tatttttctg tagggcgtga ctgcgatgcg ttccctgtcc gagttcacgc cgatctcctc
     1861 gtcactcagg tagcaggcgg ggtcttcctg ccacgcgtcc ccggtgagtt gctgcgctcg
     1921 tacccgggtg ttaccaccgc agatatcgaa gcaggagcac tcaccgcagc gccctttgat
     1981 ggttcttggg gttgccttga gcccggccat caacggatcg gaagtgtcct gccagatttc
     2041 cgagaacggc cgttcgcgta cgttgccaag aggatagtgc caccagaagg tatcggggtg
     2101 gaccacgccc agattgtcga tgttggcgac gttgacaccg ctgctgttgc cgccccattg
     2161 cgcgagtttg gcgcgcatgt ggggttccag gtgcggaaaa ttcttgcgta cccagaatag
     2221 cagatagacc ccgtcggcat cgttgttgcc ggtgacaaat tcgcggtgcc gtccttgctt
     2281 tgtatcgttc cagcaggtgt cgaacagcag gtccatcgct tttctggtgg tttccagaaa
     2341 agcatcatgg ccgcgattct tgttgccgcg tccggcgtag ttgaggtggg acagatagaa
     2401 cttgtcgatg ccttcgtcct tcaccagctg caacagtgcc ggcaggtcgt gggcattgtc
     2461 ctgcgtgagg gtgaagcgta cgccgacctt gataccggca tcacggcaga gacggatgcc
     2521 atgcagggat tccttgaacg cgcctggctt gcggcggaat ttgtcgtggg tagcctcgat
     2581 gccatccagg ctgacgccga cgtaatcaaa gccgatcccg gcaatgacgc cgatattggc
     2641 ttcggtgatg agcgtgccgt tggatgacag cccgacatag aatcccatgg ctttggcgcg
     2701 acgtgcgatg tcgtaaatgt ccgggcgcaa caatggttct ccacccgaca ggatcagtac
     2761 cggcacgcgg aaagccttga ggtcgtccat caccttgaac acttcttccg tgctcaattc
     2821 accggggaaa tccttgtcgg cggaaatcga atagcagtgc tggcaggtga ggttgcagcg
     2881 gcgtaccaga ttccagatca ccaccgggcc gggggggtta cgcctgggac cgagcggctt
     2941 gggatcggcg atttcctgca tgtactggct gattctgaac attagattct caatccggat
     3001 tttttaagga ttttcgtgct gtagagaatg gagtgggcac ggcaattcgt tccaagcagg
     3061 gccgcaatct gtctggcctt ggcttccact tccgcacggt cgcgtccgtg caccatggcg
     3121 aacagattga agcgccagtc cggcagacgg cgtggacggc ggtagcagtg gctgacaaat
     3181 tccagtgccc cgacctgctt tcccagttcg tcgacttgat cgtttatcac gtcccatacc
     3241 gtcatgccgt tggcgctcca acccagcgca tagtgattgg gcaccgcacc gatgcgacgg
     3301 atcacgccgt cgtccagcat cgcctgcatg cgccgcatca ctacctcggg cggcacgccg
     3361 agctgttcgg caatgtcatg ataaggccgc gccgtcagcg gtaatccaga ctgcgtggcc
     3421 tgcacgatgg cgcggtcgac aacgtccagt ttgaggctca tgcggtgaac ctcgcgccga
     3481 cgaaatattc tgtttctttc ggcatgttat agacagccag tccgctctcc gtttcgattt
     3541 tggcaatggc ctggctgatg ccttcaggcg tttccgtagc cagcacgaac cacatgttga
     3601 attcatgctc gcgccggtaa ttgtgcgcca cttccggcag cgcattgacg atccccgtca
     3661 cgcggtcgta atcctcttcc ggtactttca ttgccgccag gctgaaagcg ccgcccatgc
     3721 gctcgatctg gaacatgggg ccgaagcggg tcagcacttt ctccgccagc agattttcca
     3781 gccgggcaat caactcttct tcgctgatgc cgaactgctg cgccatttcc cgatacggcc
     3841 gctcgcaaat cggaaagccg ccctgcaggc cgttgacgat atcgcggtca gttgcgtcca
     3901 tctgccacat agcgtgctcc gcattgtttg aagcggcggc ggctgaacag cacctgtgtc
     3961 tgaaatgcat ccaggccggt gtttgccgcc aatgtttcga tcagtctgcg cactacatcg
     4021 cggtcggtgc catgtaccat gcagaacagg ttgaacggcc agtccggcag ctggcgcgga
     4081 cgccggtagc acagggtgac gccttcctgc tgagctgcca accggcccaa agcgctgacc
     4141 tgcccgtccg gcacatccca taccgccatg gcattttctc tgtacccaag ttcatggtgg
     4201 cgcactacca cgccgaaacg tttgatgatg ccttcgccac gccacgccga aacctggcgg
     4261 atcacgtctt cctcatccag attgacgcgt tcgccaatgg cagcgaacgg ctggcgctcc
     4321 agtttgatgc ccgattgcag cccggcgacg atggcagtct gggcgctgtc caattgcacg
     4381 cgggtgctgc tgccagatga cctgcgcgag gggcaggatc ggccgttgag atcgaaaccc
     4441 agatcgatat gaaagcgctc aaccagcggt aggaccagca tgggacaacc agattcgctg
     4501 gcgatctttt tcagcacgct atccagccag gcttggccgg gggcggcagc gacgaaccac
     4561 aggttaaagt tgtgctcgcg ttcgtaatta tggttgacct cgggcagggc gttgatccag
     4621 tgggccattt cgtccagttt tccgggcggg acggccagcg ccgccagcgt gctggcgccg
     4681 atgcagccgg gactgaacac cggcccgaca cggctcaccg caccccgtgc gctcaactgg
     4741 ccgaggcgtt cgatcacttc gtcttcttcg atgcccaact ggtcgccaat agcgagaaac
     4801 ggccgtttga ccagcgggaa atcgtgctgg tattcattca acagacgctg ttcgataagg
     4861 ttgaatccgt tcatggtcag aagccgatct ttccggaacg ataggtgaag aaaatgccgc
     4921 ttggggagga ggcgggaagt tcggcgactt gttccagtgt gccggtgtcg taaatgacga
     4981 ccttgttgtc gtcgcgtgat gacacccatg ccgcctcgcc gcgcggggta aattcaaaat
     5041 gcagcaccgc ccggcccggc tgcagcgtct gtctgacttg ctgagactcg gtgtcgatca
     5101 cctgcacctg accgttatcg ggaaaggcga aactcaccca gacctgccgt ccatccggcc
     5161 tggcgatggc gaataccggc tggccggcca ctgcaatccg gccagcctcc tgccagcttt
     5221 gcgtgtcaac caccaataac tcgtggcgcc ctatggccgg caaataggca cgtgtaccgg
     5281 ctactgccca gccgcgcaga tgtggcatct tgaataccgg cagcttttcc tcgccgtgac
     5341 catagccatc cagaatgcgc cgcgttccct gctcgggatg ccacaggtcg agcagggcca
     5401 gcccgtcctc tccgaacagt cccgcgatat agtagcggcc gtcgggcgtg atcagtccat
     5461 cgtatggctg tttgccggcg gcgaatttct gtatttccgg tgaggatgga tcggaaatat
     5521 ccgctaccca gatttcaccg gcgtcgaaca gtctgaaaac aaatctgttt cccggcgcat
     5581 cggtcaggcc gacgactttg gataactggc catcgccgta aactgccgga atatccgcga
     5641 ccagatcaag agtggctgca tcgaatacct tgacgccgcc gggcgtgtaa ttttgcgcag
     5701 caacgtagcg gccatcttgg gagatggcgc cgccgatgct gttgccggcc tgcaggatgc
     5761 gcttgtcgat gtcccgtttg agcaaatcga ccttggtcag cccgccgtcg cgtccaaaaa
     5821 cgtaggcata gcggccgtcg cgactgaata ccacggaggc atgggacaaa tcgccgaggc
     5881 cggtaatggt cgccaggctt ttgcgggcgg aagtatccac tacctgcacg cgcccgacat
     5941 cacgttcgat aacgaccccg aggtcgcccg tgccgcgtac cggtgcgccg gcacagccga
     6001 caagtgccag cagcaaaatc gccaggcacc acttattgca catccgggaa ccctttcatc
     6061 agttgctcca caatccagtt cgcttccgct tcactcagaa accttgacca gggcggcatc
     6121 gcagtgccgg cacgcccttg ctggatggtt tcgcgcaaag ctcccggcgc ctttcctgcc
     6181 aatgccgccg gcagcagcga aggccccagc ccgccgccga gcttcaagcc gtggcaggag
     6241 ccgcagtcgt tgcgcaccaa ccgcaccagt tcacgctgcc gctgctggtt gacttcttcg
     6301 ccagcatcag cacccacact gaaaaacgct atccagccga gaaaaaaagc ggggccaagg
     6361 ccccgcgaaa tttcacgaac agacatactg cgtaatcccc ggtcagtaaa cgtcgtgctg
     6421 ggtgttgtag acgttaaatt tgccggtcgg cgtgatcagc ttgggatcct tgatcacggc
     6481 cttgagcgtg cgggttttgt catccactac cacgatggcg gattccttgt ccttggcgct
     6541 ccataccgag aaccagacct catcgccggc tttgttgtat tccggctgta cgatgcgttt
     6601 ggcgccttcg cccaagccgg cccactcgcc gatcggcagc atggtgaagc ccttgtcgag
     6661 gttgtcgatg tcataaacag ctaccgactg actgaccttg gcttccgggt tgagcggagt
     6721 atccacccac agattcttcg acttgggatg ggtcttgata aacagcgaac ccccaccctg
     6781 gccattgagc gtgcgcacca ccttccatgc ctgatcct
//
LOCUS       DAOMFA010000087         6800 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_1401_length_6800_cov_5.062565, whole genome shotgun sequence.
ACCESSION   DAOMFA010000087 DAOMFA010000000
VERSION     DAOMFA010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 6800)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6800)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6800
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1401_length_6800_cov_5.062565"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..396)
                     /locus_tag="PKW44_05590"
     CDS             complement(<1..396)
                     /locus_tag="PKW44_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HQR51091.1"
                     /translation="MIETDRLIAADPLGKEEEALERALRPKVLDEYIGQEKARGQLEI
                     FINAARGRAEALDHVLLFGPPGLGKTTLAHIIAREMGVSLRQTSGPVLERAGDLAALL
                     TNLEPNDVLFIDEIHRLSPVVEEILYPAME"
     gene            complement(393..977)
                     /gene="ruvA"
                     /locus_tag="PKW44_05595"
     CDS             complement(393..977)
                     /gene="ruvA"
                     /locus_tag="PKW44_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HQR51092.1"
                     /translation="MIGRLSGVLLEKNPPQVLVDVNGVGYEVEVPMSTFYNLPDTGAK
                     VTLLTQFVVREDAQLLYGFFSQRERETFRQLVKISGIGAKSALGILSGLSVDDLAHAI
                     AAQDAALLVRIPGIGKKTAERLLLELRDKLPVSGTAAILAGSQPHAAGNDVLNALLAL
                     GYHEREALAAIKQLPAGASVSDGIRQALKVLSKS"
     gene            complement(974..1927)
                     /locus_tag="PKW44_05600"
     CDS             complement(974..1927)
                     /locus_tag="PKW44_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018292539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HQR51093.1"
                     /translation="MKAAAVTLEPTPERYANPVARYFAATRPPFLSATLVACLLGLAI
                     AAYSGIAIDKATALITALLALLVHAAVNVLNDYYDALNGTDDANTQRVFPFTGGSRFI
                     QNGVLTPSQTACFGYALLALSVLGGLWLAWQSGEGLFALGVAGLLIGWAYSAAPLRLN
                     SRGLGELCVLAGFLGVVVGADYVQRHTFSAQPWLVGLPYALLVTNLLYINQFPDRVAD
                     SAAGKRHWVVRLPLPVAARIYPLLVGLALGMLLALAQRGLIPDIALASGLPMLLSFKA
                     AAILRRHTAQPAELLPAIRLTIAAMLAHGILLAILLWWGAA"
     gene            complement(1924..2466)
                     /gene="ruvC"
                     /locus_tag="PKW44_05605"
     CDS             complement(1924..2466)
                     /gene="ruvC"
                     /locus_tag="PKW44_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HQR51094.1"
                     /translation="MKTRILGIDPGLQITGFGVIDKVGEKLAYVASGTIKTAGGKSGK
                     TEELPARLKIILTGLTEVIQTYQPTQVAIEKVFVNVNPQSTLLLGQARGAAISAAVLH
                     DLPVAEYTALQVKQAVVGNGHAAKEQVQEMVKRLLGLPAVPKPDSADALACAICHAHG
                     GQGLGKLATAGFRVRGGRLV"
     gene            complement(2466..3191)
                     /locus_tag="PKW44_05610"
     CDS             complement(2466..3191)
                     /locus_tag="PKW44_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HQR51095.1"
                     /translation="MAGHSKWANIQHRKGRQDAKRGKIFTRLIKEITVAAKLGGGDPA
                     INPRLRMAIEKAKAESMPKDNIENAVKRGSGQLEGVNYEEIRYEGYGLNGAAVMVDCL
                     TDNRQRTVADVRHAFSKYGGNLGTDGSVAFLFKHCGILLFAPGADEDKVLEAALEAGA
                     EDVVSDEDGSIEVITAPNDFSAVKEALEAAGFKPEVAEVTMKAANETVFTGDDAVKMQ
                     KLLDALDDLDDVQDVYTSALIEE"
     gene            complement(3251..4168)
                     /locus_tag="PKW44_05615"
     CDS             complement(3251..4168)
                     /locus_tag="PKW44_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HQR51096.1"
                     /translation="MPEKSAHQTRAMIALLVLAIIWGYNWVVMKVALQYSTASQFAAM
                     RTLGGGLFLLALLAAMKKPLRPREIPSTIVLGLLQTTGFTGFIIWALVSGAAGKTSVL
                     SFTMPFWVLLLAWPMLGERIRGMQWGAVALALIGLVCILEPWRMHGSMHSTILAVLAG
                     VSWALSVVFHKKLTRRVPDLDLLSFTTWQMLFGSVPIVLFALGDLDQPIQWTPSYTVA
                     IIYSVVLANALGWMAWLYALQRLPAGVASMSSMLIPVIAVLAAWLQLGERPSPQEATG
                     MVCIIAALLLISWVSIRRHERVDAAMGQD"
     gene            4286..4720
                     /locus_tag="PKW44_05620"
     CDS             4286..4720
                     /locus_tag="PKW44_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HQR51097.1"
                     /translation="MKTVRQLLESKPVGILSITSCTTVYDALVVMAENHVGALLVMDN
                     GDLAGIFSERDYARSVALKGKSSRDTPVSEIMTPRECLITVGLGDTVEYCLSLISDKR
                     IRHLPVLDHDKVAGILSIGDLVQATIEYQQFLIQQLESYIKS"
     gene            complement(4730..6100)
                     /gene="yegQ"
                     /locus_tag="PKW44_05625"
     CDS             complement(4730..6100)
                     /gene="yegQ"
                     /locus_tag="PKW44_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HQR51098.1"
                     /translation="MNNSAPELLAPAGDLDRMRAAYDFGADAVYAGQPRYSLRARNND
                     FTLQNLALGIEEAHARGKLFFVASNLMPHNAKVKTYLADMEPVIAMQPDALIMADPGL
                     IAMVREKWPEVPVHLSVQANTVNYATVKFWQALGLTRVILSRELSLDEIEEIRQQCPD
                     MELEVFVHGALCIAYSGRCLLSGYFNHRDPNQGTCTNSCRWDYKMHDAAEDAAGVIRI
                     HELDFRAEMEKSSLSDCGSLQRHPAADQVYLIEEKNRPGELMPILEDEHGTYIMNSKD
                     LRAVQHVERLVKIGVDSLKIEGRTKSRYYVARASQVYRRAIDDAVAGRPFEPALLGEL
                     EGLASRGYTDGFYQRHSTVEHQNYLRGYSESSRSQYVGDVVSYDTATGLAEIEVKNRF
                     AVGDRLEIIHPAGNREVVVESLLNEEGIPVDTAPGSGHHVRLALPPGNVEKALLARFL
                     NPSQKP"
     gene            complement(6093..6602)
                     /locus_tag="PKW44_05630"
     CDS             complement(6093..6602)
                     /locus_tag="PKW44_05630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR51099.1"
                     /translation="MKMFLQWLFLVLGIVTGQASLADEKWYRYFPQGGACLELWQPHP
                     LLKQEAKKSPVQLYYGIFKIPVPDNPPKKLPASPAEIVDFLNTGYAGKVALEPLADLF
                     RKELAQDETFKDISAEEQSKMLKKLENNAYLLTGSYHPNNAVDDSVELEKLGLVLVTQ
                     QVCEWSHSE"
     gene            complement(6654..6782)
                     /locus_tag="PKW44_05635"
     CDS             complement(6654..6782)
                     /locus_tag="PKW44_05635"
                     /inference="COORDINATES: protein motif:HMM:NF014009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HQR51100.1"
                     /translation="MGPAVLVPTSGGPLDFGPWEQIFYGEFDGRRRKRVLVKVIGE"
BASE COUNT         1294 a         2056 c         2116 g         1334 t
ORIGIN      
        1 ctccatcgcc ggatagagga tttcctcgac gacaggcgaa agacgatgaa tttcgtcgat
       61 gaacagcaca tcgttgggtt cgaggttggt cagcagcgcc gccaggtcgc ccgcgcgttc
      121 cagcaccggc cccgaagtct ggcgcaggct gacgcccatt tcacgcgcga tgatatgcgc
      181 cagcgtggtc ttgcccagcc ccggcgggcc gaacagcagg acgtggtcga gcgcttccgc
      241 acgaccgcgc gcggcgttga tgaagatttc cagctgcccg cgcgcctttt cctggccgat
      301 atattcatcc agcaccttgg ggcgcagggc gcgttccagg gcttcttctt ccttgcccaa
      361 cgggtcggcg gcgatgaggc ggtcggtttc gatcatgact tggacaatac tttcagtgcc
      421 tgccggatgc cgtccgacac cgaagcgccg gccggcagct gcttgatagc cgcaagcgcc
      481 tcgcgctcgt ggtaacccag cgccagcagg gcgttgagca cgtcgttgcc ggcagcgtgc
      541 ggctggctgc cggcaagaat ggcggcggtg cctgacaccg gcagtttgtc gcgtagttcg
      601 agcagcaggc gctcggcggt tttcttgccg atgccgggaa tgcgtaccag cagggcggcg
      661 tcctgcgcgg cgatggcgtg ggcgaggtcg tccacagata agccggagag aatgccgagt
      721 gccgacttgg cgccgatgcc gctgatcttg actaactggc ggaatgtctc gcgttcgcgc
      781 tggctgaaga agccatacag cagttgcgcg tcctcgcgca ccacgaactg ggtcagcagc
      841 gtcaccttgg cgccggtgtc cggcaggttg tagaaagtgc tcattggtac ctcgacctcg
      901 tagccgacac cgttcacatc caccagcacc tgcggcggat ttttttccag caatacgccg
      961 gacaagcgac cgatcatgca gcaccccacc acaacaaaat tgccagcagg ataccatgcg
     1021 cgagcatggc ggcgatggtc aggcggatgg ccggtaacag ttcggcaggc tgggcggtat
     1081 ggcggcgcag aatggcggcg gccttgaacg agagcagcat gggcagcccc gacgccaggg
     1141 caatgtccgg gatcaatccg cgctgcgcca gcgccagcaa catgcccaac gccaacccga
     1201 ctagcaatgg gtaaatgcgc gctgcgaccg gcagtggcag gcgcactacc cagtggcgct
     1261 tgccggcggc ggagtcggca acgcgatcgg gaaactggtt gatgtagagc aggttggtga
     1321 ccagcagtgc atacggcagg ccgaccagcc agggctgcgc ggagaaggtg tggcgctgca
     1381 cgtagtcagc gccaaccact acgccgagga aaccggccag cacgcacagt tcgcccaatc
     1441 cgcgtgaatt cagcctcagc ggcgcggcgg aataagccca gccgatcagc aatcctgcga
     1501 cccccaaggc aaacaagccc tcgccggact gccatgccag ccacagtcca cccagcacag
     1561 agagcgccag cagggcatag ccaaaacagg cggtctggga gggcgtcagc acgccattct
     1621 ggatgaagcg actgccgccg gtgaatggga acacgcgctg tgtgttggcg tcatccgtgc
     1681 cattgagggc atcgtagtag tcgttgagca cgttgactgc ggcatgtacc agcaaggcca
     1741 gtaatgcggt aatcaatgcg gtcgccttgt cgatggcgat accactgtat gccgcaatcg
     1801 ccagcccgag cagacaggca acgagcgttg cggagaggaa cggcgggcgg gtggcggcga
     1861 aatagcgcgc gaccgggttg gcgtaacgct ccggggtggg ttccagtgta acggcagcag
     1921 ccttcatacc agacgtcctc cgcggacacg gaatcctgcg gtcgccagtt tccccaagcc
     1981 ctgcccgcca tgggcgtggc agatggcgca ggccagcgca tcggcagaat ccggtttggg
     2041 tacagccggc aggccgagca ggcgcttgac catctcctgt acctgttcct tggcggcgtg
     2101 gccgttgccg accacggcct gtttgacctg cagcgcggta tattccgcca ctggcaggtc
     2161 gtgcagcacc gcggcgctga tggccgcgcc gcgtgcctgg ccgagcaaca gggtggattg
     2221 cgggttgacg ttgacgaaca ctttttcgat ggccacctgc gtaggctgat aggtctgaat
     2281 gacttcggtc aggccggtga gaatgatctt gaggcgcgct ggcaattcct cggtcttgcc
     2341 gctcttgccg ccggcagtct tgatggtacc gctggccacg taggcgagtt tttcaccgac
     2401 cttgtcgatc acgccaaagc cggtgatttg caggccggga tctatgccga ggatgcgcgt
     2461 cttgattact cctcgatcag cgcgctggtg tagacatcct gcacgtcgtc caagtcgtcc
     2521 agcgcatcca gcagcttctg catcttcacc gcatcatcgc cggtgaatac ggtttcgttg
     2581 gcggccttca tggtgacctc ggccacttcc ggcttgaagc cggcggcctc cagcgcttcc
     2641 ttaaccgcgg agaaatcgtt gggcgcggtg ataacttcga tactgccatc ctcgtcgctg
     2701 acgacatctt cggcgccggc ttccagcgcg gcttccagta ccttgtcctc atccgcaccg
     2761 ggggcgaaca gcaggatacc gcagtgcttg aacaggaacg ctaccgagcc gtcggtgccg
     2821 aggttgccgc cgtacttgct gaaagcgtgg cgcacgtcgg ctacggtgcg ctggcggttg
     2881 tcggtgaggc aatccaccat caccgccgcg ccgttgaggc cgtagccttc gtagcgaatt
     2941 tcttcgtaat tgacgccttc gagctggcct gagccacgct tgacggcatt ctcgatgttg
     3001 tccttgggca tggattcggc cttggccttt tcgatcgcca tgcgcaggcg cgggttgatc
     3061 gccgggtcgc cgccaccgag cttggcggcc acggtgattt ccttgatcaa ccgggtgaag
     3121 atcttgccgc gcttggcatc ctgacggcct ttacggtgct ggatattggc ccatttggaa
     3181 tgacctgcca tgtgtgatgg tccttacaat tacctgaaaa agtgcggaat tttaccacag
     3241 gcaaggggga ctaatcctgc cccatggcgg cgtccacgcg ctcatgccgt cgaatgctga
     3301 cccaggaaat cagcagcagg gcagcaatga tgcaaaccat accagtggcc tcctgcgggg
     3361 aggggcgctc acccagttgc agccaggccg ccagcacggc gattaccggg atcagcatgg
     3421 aactcatgct ggcaactccg gcaggcaggc gttgcagcgc atacagccag gccatccagc
     3481 ctagcgcgtt ggccagcacc acgctgtata tgatggccac ggtataactg ggcgtccact
     3541 ggatgggctg gtccaggtcg cccagcgcga acagcacaat gggcaccgag ccgaacagca
     3601 tctgccaggt ggtgaacgat agcaggtcga ggtcgggcac gcggcgcgtc agcttcttgt
     3661 ggaacaccac cgatagcgcc cagctgacgc cggccagcac ggccaggatc gtgctgtgca
     3721 tgctgccatg catgcgccac ggctccagaa tacagaccag accgatcagc gccagcgcca
     3781 cggcgcccca ctgcatgccg cggatgcgct cccccagcat cggccaggcc agcagcaaca
     3841 cccagaatgg catggtaaag ctgagtacgg aagtcttgcc tgcggcgccg ctgaccagcg
     3901 cccagatgat gaagccggta aaaccggtgg tctgcaacag gccaagcacg atggtgctgg
     3961 gtatttcgcg cggcctgagc ggcttcttca tcgccgccag caaggctagc aggaacaggc
     4021 cgccacccaa ggtgcgcatg gcggcgaact gggaggccgt ggagtattgc agcgccactt
     4081 tcatcaccac ccagttgtag ccccagatga tggcgagcac cagcaaggca atcatagcgc
     4141 gggtttggtg agcggatttt tcaggcattc agaggtgctc ctgagaaatt gaatggaacg
     4201 ctttattgca atgaagaatt tgtctacact gatcaatcaa ggccgcagtt taccatcgac
     4261 ggccattcat ccgaggagaa gcaccatgaa aaccgtgcgt cagttgcttg aatccaagcc
     4321 ggtaggaata ctttccatca cctcctgcac taccgtttac gatgccttgg tggtcatggc
     4381 ggaaaaccat gtcggcgcct tgctggtcat ggataacggc gatctggccg gcatcttctc
     4441 cgaacgcgac tatgcacgta gcgtcgcgct caagggcaag tcgtccagag acaccccggt
     4501 cagtgaaatc atgacgccgc gggaatgcct tatcaccgtc ggccttggcg ataccgtgga
     4561 gtattgcctc agcctgatct ccgataaacg catccgccac ctgccggtac tggatcatga
     4621 caaggtcgcc ggcattcttt ccatcggcga tctggtgcag gccaccatcg aataccagca
     4681 attcctgatt caacagttgg agagctatat caaaagctga gggttggttt tacggttttt
     4741 gcgatggatt caaaaaccga gccaacagcg ctttttccac attgccgggt ggtagcgcca
     4801 gtctgacatg gtggccgctg ccaggggcag tgtccacagg tataccttct tcattcaaca
     4861 ggctttccac caccacttcg cggttaccgg cgggatggat gatttccaga cggtcgccga
     4921 cggcgaagcg attctttact tcgatttccg ccagtccggt tgccgtgtcg taactcacca
     4981 cgtcccccac gtactggctg cggctggatt ccgaatagcc gcgcagatag ttctggtgtt
     5041 ccacagtgga atggcgctgg tagaagccgt cggtgtagcc gcggctggcc aggccttcca
     5101 gttcgcccag cagggcgggc tcgaacggtc ggccggccac ggcatcgtca atggcgcggc
     5161 ggtatacctg gctggcacgt gccacgtagt agcgcgactt ggtacggcct tcgatcttga
     5221 gcgaatcgac gccgatcttc accaggcgtt cgacgtgctg caccgcgcgc aggtccttgc
     5281 tgttcatgat gtaggtgccg tgctcgtctt ccaggatggg catcaactcg ccggggcggt
     5341 tcttttcctc gatcaggtag acctgatctg cggccgggtg gcgctgcagg ctgccgcaat
     5401 ccgacagcga ggacttttcc atctcggcgc ggaagtcgag ttcgtggatg cggatgacgc
     5461 cggcggcgtc ttccgctgca tcgtgcatct tgtaatccca gcggcaggag ttggtacacg
     5521 ttccttggtt cggatcgcga tggttgaaat agccggacag cagacagcgc ccggaatagg
     5581 cgatgcaaag cgcgccgtgg acgaacactt ccagttccat gtccgggcac tgctggcgga
     5641 tttcttcgat ttcgtccagc gacagttcgc gcgacaggat cacccgcgtc agccctagcg
     5701 cctgccagaa tttgaccgtg gcgtagttga cggtattggc ctgcaccgac agatgcaccg
     5761 gcacttccgg ccatttctcg cgcaccatgg cgatcagtcc cgggtcggcc atgatcagcg
     5821 cgtccggctg catggcgatg accggttcca tgtccgccag gtaagtcttc actttggcgt
     5881 tgtgcggcat caggtttgac gcgacgaaga acagcttgcc gcgagcatgc gcttcttcga
     5941 tgccaagagc cagattctgc agggtaaagt cgttgttacg cgcccgcagg gaatagcgcg
     6001 gctggccggc atacaccgcg tcggcgccga agtcataggc ggcgcgcatg cggtcgaggt
     6061 cgccggcggg ggcgagaagt tcgggggcgg aattattcac tgtgtgacca ctcgcaaacc
     6121 tgctgcgtga ccagcaccag tcccagtttt tccagctcca cgctgtcatc gacggcgtta
     6181 ttggggtggt agctgccagt cagcaggtag gcgttgtttt ccagtttttt caacatcttg
     6241 gactgctcct cggcactgat atccttgaac gtctcgtcct gcgccagctc tttacgaaac
     6301 aagtccgcca gtggttccag cgccaccttc ccggcatagc cggtattgag gaagtccacg
     6361 atttcagccg gactggccgg tagctttttg ggcgggttgt caggaacggg tattttaaaa
     6421 atgccgtaat acagttgcac cggactcttt ttcgcctcct gcttgagtag cgggtgcggc
     6481 tgccacagtt cgagacaggc accgccctgg ggaaaatagc gataccactt ttcgtcggcc
     6541 aggctggcct gcccggtcac aatccccaga accagaaaca gccattgcag gaacattttc
     6601 atgatgcgtg atccgccggg cttcgacttg cggctcagca ccagcctgag gttttactcg
     6661 ccgatcacct tgaccagcac gcgcttgcgg cggcggccgt cgaactcgcc gtagaatatc
     6721 tgctcccagg gaccgaaatc gagggggccg ccactggtgg ggaccaggac tgcggggccc
     6781 atggtgtggc gcgggggtag
//
LOCUS       DAOMFA010000088         6764 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_1416_length_6764_cov_4.500969, whole genome shotgun sequence.
ACCESSION   DAOMFA010000088 DAOMFA010000000
VERSION     DAOMFA010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 6764)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6764)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6764
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1416_length_6764_cov_4.500969"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..498
                     /locus_tag="PKW44_05640"
     CDS             <1..498
                     /locus_tag="PKW44_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HQR51101.1"
                     /translation="AFNIDANANLHVSAKDKATGKESNITIKASPGLSEEEIRRMEED
                     AAAHADEDKKLRELVDARNTADGMVHSVKKSLTEYGDKLEAGEKEKIEAAIKEVEEVL
                     KSDDKYAYDAKTNALMEASHKLAEKMYAQQPQEGGAEPQPQGEKTVDAEVVDAEFEEV
                     KDDKK"
     gene            588..1712
                     /gene="dnaJ"
                     /locus_tag="PKW44_05645"
     CDS             588..1712
                     /gene="dnaJ"
                     /locus_tag="PKW44_05645"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HQR51102.1"
                     /translation="MAKRDYYEVLGVNRDASDDDIKKAYRKLAMKHHPDRNPDNPKAE
                     EHFKEAKEAYEILSDEQKRAAYDQYGHAGVDPQAGMGGGGFGGAGNFADAFGDIFGDI
                     FGGGGGNRRSNVYRGADLRYNMEISLEDAARGTETKIRIPVMAECETCHGSGARPGTS
                     PVTCTTCGGHGQVRMQQGFFSIQQTCPKCHGSGKMVKEPCPTCHGDGRVKQHKPLAVK
                     IPAGVDEGDRIRLSGEGEAGVNSGPPGDLYVVVHLKPHRIFQRDGGDLHCEMPISFTV
                     AALGGEIEIPTLDGHAKMKIPAETQTGAKFRLRGKGIKPLRTSEHGDLYCHVVVETPV
                     KLTERQKELLREMEEINQQDAQKHSPRAKSWMEKVKDFFQ"
     gene            1869..2594
                     /locus_tag="PKW44_05650"
     CDS             1869..2594
                     /locus_tag="PKW44_05650"
                     /inference="COORDINATES: protein motif:HMM:NF025625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HQR51103.1"
                     /translation="MLSREESDSSGIYFLTGADPESGKSAIYIGEAENIRDRIKAHLE
                     KDFWNQIVFFTSKDENLTKAHIRYLEGKLIDQARKAGRAVVTNGQSSGARLPESDRED
                     MEIFLEKINQLLPVLGVELLVPAVARAVGESEHKKLFCEIKGIKAEGHLAPNGFVVLK
                     GSQAVLTDRASSQKWPWAMNMRQRLKDEGVLETKGDALVFTSDAEFTSPSSAAAVIHG
                     GHANGLTAWKDKSGRTLKEIESA"
     gene            2706..2894
                     /locus_tag="PKW44_05655"
     CDS             2706..2894
                     /locus_tag="PKW44_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HQR51104.1"
                     /translation="MKVNVGGIERVLRVAVGLGLVAWALTGGPVWAWIGVVPIVTGAI
                     GNCPAYSIFGISTCSAKK"
     gene            complement(3195..3410)
                     /locus_tag="PKW44_05660"
     CDS             complement(3195..3410)
                     /locus_tag="PKW44_05660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR51105.1"
                     /translation="MRIIFLLVLAMLAGCASMTKSHSDRTDHKVPVCHKGKTLYVDKA
                     AVDAHIKHGDYGRACYGEKKQGDDQKD"
     gene            complement(3458..3853)
                     /gene="gloA"
                     /locus_tag="PKW44_05665"
     CDS             complement(3458..3853)
                     /gene="gloA"
                     /locus_tag="PKW44_05665"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019915435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004462 - lactoylglutathione lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0051596 - methylglyoxal catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HQR51106.1"
                     /translation="MRILHTMLRVGDLDRSIKFYSEILGMQVLRREDYPDGKFTLAFV
                     GYGGERDHTVLELTYNWGVEKYDLGSAFGHIAIEVDDAYKTCEQVRQSGGKVIREAGP
                     MKHGTTVIAFIEDPDGYKIEFIQKGTAGY"
     gene            3910..4683
                     /locus_tag="PKW44_05670"
     CDS             3910..4683
                     /locus_tag="PKW44_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018507595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent peptidase"
                     /protein_id="HQR51107.1"
                     /translation="MGLFRNWRRKRTLRQHPIPHDAWHAAVEALPLLYGLDGIELVRL
                     RELATLFLHEKSLEPVQGLQLDDAMRYDLAAQAVLPILHLGINWYDGWQSLVLYPDEF
                     VTRQQWTDEAGLVHSRREIRIGEAWERGPVVLSWADVAASGNRDGYNCVIHELAHKLD
                     YSNGAMDGCPALHDGMRVRDWRLAFEPAFEDMRRRVERGQETALDPYAVEAPEEFFAV
                     MSEHFFETPHLLTAEYPAVYEQLRQFYRQDPAARLISWP"
     gene            complement(4667..4918)
                     /locus_tag="PKW44_05675"
     CDS             complement(4667..4918)
                     /locus_tag="PKW44_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhL family 4Fe-4S dicluster ferredoxin"
                     /protein_id="HQR51108.1"
                     /translation="MALMITDECINCDVCEPECPNEAIFQGQEIYEIDPDKCTECVGH
                     YAKPQCQEVCPIDCIPFNPEYRESKDQLMEKYSRLTAKK"
     gene            complement(5065..5550)
                     /gene="coaD"
                     /locus_tag="PKW44_05680"
     CDS             complement(5065..5550)
                     /gene="coaD"
                     /locus_tag="PKW44_05680"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HQR51109.1"
                     /translation="MKPKAVYPGTFDPLTRGHEDIVRRAAGLFSEVVVAVAENTSKQS
                     FFNLQERVEMAMAVLADCPNVRVTGFSGLLMQFVREQGAGVVVRGLRAVSDFEYEFQL
                     AGMNRKLFPEVETLFLTPAEQYMFISASLVREIAVLGGEVSQFVSPLVEARLKQKIRG
                     A"
     gene            complement(5540..6082)
                     /gene="rsmD"
                     /locus_tag="PKW44_05685"
     CDS             complement(5540..6082)
                     /gene="rsmD"
                     /locus_tag="PKW44_05685"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HQR51110.1"
                     /translation="MNTVRIGAGEWRSRLLRFPDALGLRPTPDRVRQTLFNWLGQEMY
                     GKACLDLFAGSGALGFEALSRGASSVVLVEQNPAVHRALTANAALLKAERARILRMDA
                     LQFLAQDTQAFDVIFLDPPFGQDWLQKLLPQLAGHLAPDGAVYVEAEAPLAEPAGWEI
                     HKHGRAGNVCYHLLKLRHET"
     gene            complement(6137..>6764)
                     /locus_tag="PKW44_05690"
     CDS             complement(6137..>6764)
                     /locus_tag="PKW44_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HQR51111.1"
                     /translation="NLPVGAAQSVTPPVPLPTAAVEQRIAHPAAQSHILLGYPGMRRG
                     DPDYFPLYVGNYVLGGGGFVSRLTEEVREKRGLAYSVYSYFLPLRQDGPFQIGLQTRR
                     EQTAQALALVRETLKKFIQDGPSAKELKAAKDNIIGGFPLRIDSNGKILDYLAVIGFY
                     QLPLTYLDDFNKQVDKVTTAQIRDAFKRRIDPDKMVTVIVGDSGPESK"
BASE COUNT         1436 a         2009 c         2045 g         1274 t
ORIGIN      
        1 gcttttaaca ttgatgccaa cgccaatctg cacgtgtcgg ccaaagacaa ggcgactggc
       61 aaggaaagca acatcaccat caaggccagc cccggcctga gcgaggaaga gattcgtcgc
      121 atggaggaag atgcggcggc ccacgccgac gaggacaaga aactgcgcga actggtggac
      181 gcccgcaata ccgccgacgg catggtgcat agcgtcaaga agtcgctcac cgagtacggc
      241 gacaagctgg aggccggcga aaaggaaaag atcgaagccg ccattaagga agtggaagaa
      301 gtcctcaaat ccgatgacaa gtacgcctac gacgccaaga ccaacgccct gatggaagcc
      361 tcgcacaagc tggcggaaaa gatgtacgcc cagcagccgc aggaaggcgg cgccgagccc
      421 caaccgcaag gcgagaagac ggtcgacgcc gaagtggtgg acgccgagtt cgaggaagtc
      481 aaagacgaca agaagtaatc cttttaaccc gaaccgggcg cggagcacgc ggcaatacgc
      541 cgttaggggt tccgtgcccg ctgtcttaac ggcgacataa ccacaacatg gcaaaaagag
      601 attactacga agtcctcggc gtcaatcgcg acgcttcgga cgatgacatc aagaaggcct
      661 accgcaaatt ggccatgaag caccatcccg accgcaatcc ggacaatccc aaggccgagg
      721 aacacttcaa ggaagccaag gaagcctacg aaatcctgtc cgacgagcag aagcgcgcgg
      781 cctatgacca gtacggccac gccggggtcg atccgcaggc cggcatgggc ggcggcggtt
      841 tcggcggggc aggcaatttt gccgatgctt tcggcgacat cttcggcgac atcttcggcg
      901 gcggcggtgg caacaggcgt tccaacgtct atcgcggcgc cgacctgcgc tacaacatgg
      961 aaatctcgct ggaggacgcc gcacgcggta ccgagaccaa gatccgcatc ccggtcatgg
     1021 ccgagtgcga aacctgccac ggctccggtg cgcgacccgg cacctcgccc gtcacctgca
     1081 ccacctgcgg cgggcacggc caggtgcgca tgcagcaggg cttcttctcc atccagcaga
     1141 cctgccccaa gtgccacggc agcggcaaga tggtcaagga gccgtgcccc acctgccatg
     1201 gcgacggccg cgtcaagcag cacaagcccc tggcggtcaa gattcccgcc ggtgtggacg
     1261 agggcgaccg catccgcctg tcaggcgagg gcgaagccgg cgtcaacagc ggtccgcccg
     1321 gagatctgta cgtggtggtg cacctcaagc cgcacaggat cttccagcgc gacggcggcg
     1381 atctgcactg tgaaatgccg atcagcttca ccgtggcggc tttgggcggc gaaatcgaaa
     1441 tccccaccct cgacggccac gccaagatga agatcccggc ggaaacccag accggcgcca
     1501 agttccgtct gcgcggcaag ggcatcaaac ccctgcgcac cagcgaacac ggcgaccttt
     1561 actgccacgt cgtggtggaa accccggtca agctcaccga acggcagaag gaactgctgc
     1621 gcgaaatgga agaaatcaac cagcaggacg cgcagaaaca cagcccacgg gccaagtcgt
     1681 ggatggagaa ggtgaaggat ttttttcagt agaatgatgg ctattccacg ttaatatgaa
     1741 tttcgtgcat gcctagtgca acaatcaaaa tattcctagt tcacggagat cccaagcgac
     1801 tccgaactgc ggaattgtcg aactggactg ggaaggctgt tgccggcccc agaagtgagt
     1861 ttgacggtgt gctgtcccgt gaagagtcag acagttcggg aatctatttc ctaactggag
     1921 ctgatcccga atctggaaag tcagcaatct atattggcga agctgaaaac attcgtgacc
     1981 gaatcaaggc gcacttggaa aaggacttct ggaaccaaat tgtctttttt accagcaagg
     2041 acgagaactt aactaaggcg cacatacgct acctcgaggg aaagctaatc gaccaggcgc
     2101 gtaaagccgg gcgggccgtg gttacaaacg gtcaaagtag cggggctcga cttcccgagt
     2161 ctgatagaga ggacatggag atatttctag agaaaatcaa ccagttgtta cctgttcttg
     2221 gggttgaact gcttgtaccg gctgtagcaa gggctgtagg cgagtctgaa cataagaagt
     2281 tattctgcga aatcaaggga atcaaagctg aggggcatct ggcaccaaat ggctttgtag
     2341 tacttaaggg atcacaagcc gttctaaccg accgggcgtc atcgcaaaaa tggccttggg
     2401 caatgaatat gcggcagcga ttaaaggacg aaggcgtgtt ggagacaaaa ggcgatgctt
     2461 tggtgttcac gagcgatgca gagttcacta gcccaagttc agctgcggcg gtgattcatg
     2521 gaggacatgc gaacggcctt accgcttgga aagacaaaag tgggagaact ctgaaagaaa
     2581 tagaatccgc ataaccaatc attcccccgg aaatcccttt caaatatttg ccactgcagc
     2641 cacacctcgc tttcccaaac aatggtctaa tacccttacc ttcattaccc ccaaggagtc
     2701 tagccatgaa agtgaatgtc ggcggtatcg agcgtgtctt gcgcgtcgca gtcggtctgg
     2761 gattggtcgc ctgggcattg acgggcggcc cggtctgggc atggatcggc gtggtgccta
     2821 tcgtgaccgg cgcgatcggc aattgcccgg cctattccat tttcggcatc agcacctgct
     2881 cggccaagaa ataggcacgg tcctaccggc aaacaaaaag gccaaaccct gcggtttggc
     2941 cttgtagtca ctgcgtcccg gcgtttacgc cagcttgatg atccagccgg cgaccagcac
     3001 tgccagcacc cccaccagcg tatagatgaa ataccagact tcgtgcggga tcggctgcgg
     3061 gccctgcatc tgatggtcag acatatgcgc ctccttcttt caggttttat ccaggaagaa
     3121 aacagctaat gcactgacta tactagtaaa aatagcctcg ttccgcaacc tggaatcacc
     3181 ggccaggctg agcatcagtc tttctggtca tcgccttgtt tcttctcccc gtagcatgcc
     3241 ctgccataat ctccgtgctt tatatgggcg tccaccgcgg ctttgtcgac gtagagtgtt
     3301 tttcccttgt ggcagactgg tactttgtga tccgtacgat ccgagtggct cttggtcatc
     3361 gaggcacagc cggcaagcat cgccaggacg agcagaaaaa taattctcat gggttactcc
     3421 tgaattcaga taacgacagg gttaatgcag gcggcgatca gtagcccgcc gtgcccttct
     3481 ggatgaactc gatcttgtag ccgtccgggt cttcgatgaa ggcgatcacc gtggtgccat
     3541 gtttcatcgg gccggcttcg cggatcacct tgccgccgga ttgtctgact tgttcgcaag
     3601 tcttataggc gtcgtccacc tcgatggcga tatggccgaa cgcgctgccg aggtcgtatt
     3661 tttccacgcc ccagttatag gtcagctcca gcacggtgtg gtcgcgctcg ccgccgtagc
     3721 cgacgaaggc cagggtgaac ttgccgtccg ggtagtcctc gcgccgcagc acctgcatgc
     3781 cgaggatttc gctgtagaac ttgatggaac ggtccaggtc gccgacccgg agcatggtgt
     3841 gcaggatgcg catggtgttt tcctcaacga tacaaaacga tgtgcatatt atcgccgtaa
     3901 aataggcgca tgggtttatt caggaactgg cgccgcaagc gcactctgcg gcagcacccg
     3961 atcccgcacg atgcctggca cgcagcggtg gaggcgctgc ccttgctgta cggcctggac
     4021 ggcatcgagt tggtgcggct gcgcgagctg gccacgctgt tcctgcacga aaagagcctg
     4081 gagccggtgc aggggctgca actcgatgac gccatgcgct acgacctggc cgcgcaggcg
     4141 gtgctgccca tcctgcatct tggcatcaac tggtacgacg gctggcaatc gctggtgctg
     4201 tacccggacg aattcgtcac ccgccagcag tggacggacg aggccggcct ggtgcattcg
     4261 cgccgcgaaa tccgcatcgg cgaagcttgg gagcgcggcc cggtggtgct gtcctgggcg
     4321 gacgtggcgg cttccggcaa ccgcgacggc tacaactgcg tgattcacga actggcgcac
     4381 aagctggatt attccaacgg cgccatggac ggctgcccgg cgctgcatga cgggatgcgg
     4441 gtgcgcgact ggcgcctggc gttcgagccg gcattcgagg atatgcgcag gcgcgtggag
     4501 cgcgggcagg agacagcgct cgatccctac gcagtcgagg cgccggagga gttcttcgcc
     4561 gtgatgagcg agcatttctt cgaaacaccg cacttgctca cggcggaata cccggcggtc
     4621 tacgaacaac tgcggcagtt ttaccggcag gacccggcgg cccggcttat ttcttggccg
     4681 tgaggcggga atacttctcc atcagctggt ccttgctctc ccggtattcc ggattgaatg
     4741 ggatgcagtc gatggggcag acttcctggc actgtggctt ggcgtagtgg ccgacgcatt
     4801 cggtacactt gtcgggatcg atctcgtaga tttcctgccc ctggaaaatc gcctcgttcg
     4861 ggcactccgg ctcgcacaca tcgcagttga tgcattcgtc ggtaatcatc aacgccatat
     4921 gggtgcccta aaaatccatt ttacgggcgc atcgcagcgt tgcgcggtgc tcgcccgctc
     4981 gccgtactcc atgtactgtc tcacgttctg cgctccgtgc gccttgcgct gcatcccgca
     5041 aaatgaattt atcgaggcac ccattcaagc cccacggatc ttttgcttca gccgcgcttc
     5101 gaccagcggc gaaacgaact gcgacacctc gccgcccagc acggcgatct cgcgcaccag
     5161 gctggcggag atgaacatgt attgttcggc cggggtgagg aacagggttt cgacttcggg
     5221 aaagagcttg cggttcatgc cggccagctg gaactcgtac tcgaagtcgg acacggcgcg
     5281 caggccgcgt accaccacgc cggcgccctg ttcgcgcacg aactgcatca acaggccgga
     5341 aaatccggtc acgcgtacgt tggggcagtc ggccagtacc gccatcgcca tttccacgcg
     5401 ctcctgcagg ttgaaaaagg attgcttcga ggtgttctcc gccaccgcga cgactacttc
     5461 ggaaaacagc ccggcggcac ggcgcacgat atcctcgtga ccgcgggtga gggggtcgaa
     5521 ggtgccggga tagaccgctt taggtttcat ggcgcaattt tagcaggtga tagcaaacat
     5581 tgcccgccct gccgtgcttg tggatttccc agccggccgg ttccgccagc ggcgcctcgg
     5641 cttccacgta caccgcgccg tccggcgcca gatggccggc tagctgtggc agcagctttt
     5701 gcagccagtc ctggccgaag ggcgggtcga ggaagatgac gtcgaaggct tgcgtatcct
     5761 gcgccaggaa ctgcagggca tccatgcgca ggatgcgtgc ccgctcggcc ttgagcaggg
     5821 cagcgttggc cgtcagcgcg cggtgcactg cagggttctg ttccaccagc accaccgagc
     5881 ttgcgccgcg cgacagcgcc tcgaagccca gcgcgccgct gcctgcgaac aggtcgaggc
     5941 aggccttgcc gtacatttcc tgccccagcc agttgaacag cgtctgccgc acacgatccg
     6001 gcgtcgggcg caggccgagc gcgtcgggaa agcgcagcag gcggctgcgc cattcaccgg
     6061 cgccgatgcg tacagtattc atccggctcg caatcaaaat cagggcaagg cgctgaagac
     6121 gaagccaact cgaccatcat tttgattccg ggccggagtc gccgacaatc accgtcacca
     6181 tcttgtccgg gtcgatgcgg cgcttgaacg catccctgat ctgcgccgtc gtcactttgt
     6241 cgacctgttt gttgaaatcg tccaggtagg tcagcggcag ctggtagaag ccgatcaccg
     6301 ccaggtagtc gaggatcttg ccgttgctgt cgatacgcag cgggaagccg ccgatgatat
     6361 tgtccttggc cgctttcagt tccttggcgc tggggccatc ctggatgaat ttcttcagtg
     6421 tttcgcgtac cagcgccagc gcctgggcgg tctgctcgcg cctggtctgc aggccaatct
     6481 ggaacggccc atcctgccgc agtggcagga agtagctgta gacgctgtag gccagaccgc
     6541 gcttctcgcg cacttcttcg gtcaggcgcg agacaaagcc gccaccgccg aggacgtaat
     6601 tgcccacgta cagcgggaaa tagtccgggt cgccgcgccg catgccggga tagcccagca
     6661 ggatgtgcga ctgtgcggcc gggtgggcga tgcgttgctc caccgcggcg gttggcagcg
     6721 gtaccggcgg ggtcacgctc tgcgctgcgc cgaccggcag attc
//
LOCUS       DAOMFA010000089         6746 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_1427_length_6746_cov_5.217157, whole genome shotgun sequence.
ACCESSION   DAOMFA010000089 DAOMFA010000000
VERSION     DAOMFA010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 6746)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6746)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6746
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1427_length_6746_cov_5.217157"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..913)
                     /locus_tag="PKW44_05695"
     CDS             complement(<1..913)
                     /locus_tag="PKW44_05695"
                     /inference="COORDINATES: protein motif:HMM:NF013646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HQR51112.1"
                     /translation="MNPGHEFDVLVVGGGLVGAACALALGQGGLRVGLLESAAPLALP
                     RDDSWDSRIYAVTPGNVSWLRGMGTWDTLEAARIAPIHRMEVWGDDGHSKLEFDAYES
                     GAAELGVIAESRLMLDGLWRQLQACGRVELLCPARPAALEIGADAAVLTLEDGRMLHA
                     KLLVGADGAQSWVRQQAGIEPLFARDYGQWGVVANFACEKPHGNVARQWFSKDGILAW
                     LPLPGDRISMVWSTPEPHARELLALEAQALCAAVAEAGGMALGALQLLTPAAGFPLKL
                     QYNESLIRPRLALIGDAAHRVHPLAGQG"
     gene            complement(910..2091)
                     /locus_tag="PKW44_05700"
     CDS             complement(910..2091)
                     /locus_tag="PKW44_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQR51113.1"
                     /translation="MGRARGQDTDLVIVGGGPVGATLALALAGSGLDVTVLEARPDAA
                     PPTDPRAIALSYGSRLILQRLGVWDALAPATTPIRAIHISQQARLGRSVLTAQESGQP
                     ELGCVVDYGALYGALSGALEASGIPVLYGAQATALATSTDAATVTFTRQGVEQQRSAA
                     LAVVADGGRSLKEIPGIRKEAHDYGQTALVARVETELPHGNVAYERFTPRGPLALLPW
                     GERAFALVWTETPEAAQALTTLNKADFLQQLHTHFGDRQGRFVKVQDRGVFPLKLARA
                     RPVATQRLAVIGNAAQTLHPVAGQGFNLGLRDAWELAQILAATPQGQLGGSTMLARYQ
                     SGRRADTGGGILFTDFLVRTFSNDIPGFGAVRGYGLAALELLAPAKRFVVGKMSFGAR
                     G"
     gene            complement(2147..3445)
                     /locus_tag="PKW44_05705"
     CDS             complement(2147..3445)
                     /locus_tag="PKW44_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P N-terminal domain-containing
                     protein"
                     /protein_id="HQR51114.1"
                     /translation="MSHQPYSSRRARLLAQLGDGIAVLPTAPEKLRNRDAHHPYRFDS
                     YFWYLTGFPEPESVLVLVGGKRPQSILFCRDKNEEREIWDGFRHGPQGACEAFGFDAA
                     YPIAELDTRLGELLADRPALWYSLGHDVEWDARITAALNAVRAQARAGKRAPAEIRDL
                     RVPLDAMRLTKDAHELDAMRRAAAISSAGHARAMRACRPGIAEYELEAELTYEFRRRG
                     AEAHAYTPIVAGGLNACVLHYVANDKLLNDHELVLIDAGCEVEGYASDITRTFPVNGR
                     FSAPQRDAYEIVLAAQAAAIAATKPGNHFMDPHQAALRVLAQGMLDLKLLTGSLDGAL
                     ESEAYKRFYMHRTGHWLGLDVHDAGEYKEGDAWTTLAPGMTLTVEPGLYIRPAADVPP
                     HLAGIGIRIEDDVLVTPAGCEVYTSAPRTVAEIEEVMRRD"
     gene            complement(3442..4107)
                     /locus_tag="PKW44_05710"
     CDS             complement(3442..4107)
                     /locus_tag="PKW44_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HQR51115.1"
                     /translation="MKAMILAAGRGERMRPLTDHMPKPLLPVGGKPLIAWHIERLAAA
                     GFRELVINHAHLGIQIESALGNGSRFNVSIQYSPEGEALETAGGIANALPLLGEAPFL
                     VVNGDIFTDYDFARLNHSLGEGLLAWLVLVDNPDHNGNGDFSLWGNRLTEQGREMLTF
                     SGIGVYHPALFASVQHGAKAKLAPLLREAMRSGKVGGEYYGGRWTDVGTPQRLAELDA
                     TLK"
     gene            complement(4107..5111)
                     /locus_tag="PKW44_05715"
     CDS             complement(4107..5111)
                     /locus_tag="PKW44_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018507027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HQR51116.1"
                     /translation="MQRLEQLQRWLRNALHGESFSLAPASADASFRRYFRVTLPDRTL
                     IAMDAPPQHENCEPFLHVAELFAAAGVNVPRILDQDLAQGFLLLTDLGNTTYLTALNE
                     QTAPGLYRDATEALVRIQQGSRPDTLPPYDEALLTREMRLFPEWYVGKHLGTTLDDKQ
                     QAVLQQTFDVLNRNILAQGQVYVHRDYHSRNLMVCDDATGSNPGILDFQDAVYGAITY
                     DLVSLLKDAYIRWEEAQVLDWVVRYWQQARKTGLPVPDDISTFYRDFEWMGAQRHLKV
                     LGIFARLYHRDGKEGYLKDLPLVMDYLRRACERYIELRPLLHLLDQLENRQIQVGYTF
                     "
     gene            5291..>6746
                     /gene="lptD"
                     /locus_tag="PKW44_05720"
     CDS             5291..>6746
                     /gene="lptD"
                     /locus_tag="PKW44_05720"
                     /inference="COORDINATES: protein motif:HMM:NF040057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0010033 - response to organic substance
                     [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HQR51117.1"
                     /translation="MQTFRNLAHAFVLLTAMSGQTQAAENSPAEAPDADEITIDAQRL
                     EVYLDRKMSAIGNAEVHRSDQVLYGDRIDYDMINEELHVSGNSRMVQGGTVVTGPDLR
                     MKLGEREGEMQDPVFTMRNTKALVGASTRSGKTARPGDISFARGSAKSVTLDGPDRER
                     FTDARYTSCEEGVDDWYLRAKDLELDHSTDTATAKNASIEFKGVPILYTPWIDFPFSG
                     QRKSGFLSPTFGTTSLNGAEFALPYYWNIAPDMDATITPRYMSKRGTMLGGDFRYLGE
                     QYSGRDNLDYLQHDDLTGQTRWFADLEHKQNFGNGWSGSYDIKRVSDDKYLSDMTTNI
                     VATSTVNLPQQGILSYGTSSLNSSFNFNAIAQEFQTLDNKSYPYFRLPEFNLSASRDW
                     DNVTGNMYAQWVSFEQSNDAPDNVKGSRLTSYPSFSLPLNSSYGYIVPKLGAKYWTYN
                     LSGNTVIPGTTENYQSDSTALPIFSVDSGVYFDRN"
BASE COUNT         1242 a         2137 c         2125 g         1242 t
ORIGIN      
        1 cgccctgtcc ggccagcgga tgcacgcggt gggcggcatc gccgatcagc gccaggcgcg
       61 gccggatcag gctttcgttg tattgcagct tgagtgggaa ccccgccgcc ggtgtcagca
      121 gttgtagtgc tcccagcgcc atgccgccag cctcggccac cgccgcgcac agcgcctgtg
      181 cttccagcgc gagcagttcc cgcgcgtgcg gctccggcgt cgaccacacc atcgaaatgc
      241 ggtcgcccgg cagcggcagc caggccagga tgccatcctt gctgaaccac tgccgcgcaa
      301 cattgccgtg cggcttttcg caggcgaagt tcgccactac accccattgc ccgtagtcgc
      361 gcgcgaacag tggttcaatc ccggcctgct gtcgcaccca ggactgcgcg ccgtcggcgc
      421 cgaccagcag tttggcgtgc agcatgcggc cgtcttccag cgtcagcacg gcggcatccg
      481 ctccgatttc cagcgctgcc ggacgcgccg ggcacagcag ctcgacccgg ccgcaagcct
      541 gcaactgccg ccacaagcca tccagcatca gccggctttc ggcgatcacg cccagttccg
      601 ccgcgccaga ttcgtaggcg tcgaattcca gcttggaatg gccatcgtcg ccccagactt
      661 ccatccggtg aatgggcgcg atgcgcgcgg cctccagcgt atcccacgtg cccatgccgc
      721 gcagccagct cacgttgccg ggcgttaccg cgtagatgcg gctgtcccag ctgtcgtccc
      781 gcggcagcgc caatggcgcg gcgctttcca gcagcccgac gcgcaggccg ccctgcccca
      841 gcgccagcgc acaggcggcg ccgaccaggc cgccgccgac caccaatacg tcgaattcgt
      901 gtccggggtt catccgcgtg ccccgaagct catcttgccc accacgaagc gcttggctgg
      961 cgccagcagt tccagcgccg ccagcccgta gccgcgcacg gcgccgaagc cggggatgtc
     1021 gttggaaaag gtgcgcacca ggaaatcggt gaacaggatg ccgccgccgg tatccgcgcg
     1081 gcggccggac tggtagcgtg ccagcatggt gctgccgccc aattggcctt gtggtgtcgc
     1141 ggcgagtatc tgcgccagtt cccaggcatc gcgcaagccg aggttgaagc cttgcccggc
     1201 caccgggtgc agggtctgcg ccgcgttgcc aatgaccgcc agccgctgtg tcgccaccgg
     1261 cctggcgcgc gccagtttca gtggaaatac gccgcgatcc tgtactttga cgaagcgccc
     1321 ctggcggtcg ccgaaatgcg tatgcaactg ctgcaaaaaa tccgccttgt tcagtgtggt
     1381 cagggcttgc gccgcttccg gagtttccgt ccataccagg gcgaaggcgc gctcacccca
     1441 tggcagcagc gccagcggcc cacgtggagt gaaacgctcg taggccacat tgccgtgcgg
     1501 cagttcggtt tccacccgcg ccaccagtgc tgtctggccg taatcgtggg cttctttccg
     1561 gatgccggga atttccttca ggctgcgacc gccgtcagct actaccgcca gtgccgcact
     1621 gcgctgctgc tccactccct gccgggtaaa agtgacggtc gccgcatcgg tgctggtcgc
     1681 cagggccgtg gcctgtgcgc cgtacagaac gggaatgccg gacgcttcca gcgccccgga
     1741 cagggcgccg tagagcgcac cgtagtccac cacgcagccc agttccggct ggccggactc
     1801 ttgtgcggtc aacacgctgc gtcccagccg cgcttgctgc gaaatgtgga tggccctgat
     1861 cggcgtagtg gcgggcgcca gtgcatccca tacccccagc cgttgcagga tcaggcggct
     1921 gccgtaggac agggcaatcg cgcgcggatc agtgggcggc gcggcatcgg gacgggcttc
     1981 gagtacggtg acatccagcc cgctgccggc cagcgccaag gccagcgtgg cgccgaccgg
     2041 gccgccgccg acgatcacca gatcggtgtc ttgccccctc gccctgccta tggcacccct
     2101 ctccccatgg gtggggagag gggtggaggt gaggggggtt tgtttttcag tcacgccgca
     2161 tcacctcttc gatttcggcc acggttctcg gcgcgctggt gtagacctcg cagccagccg
     2221 gcgtcactag cacatcgtcc tcgatgcgga tgccgatgcc ggccagatgt ggcggaacgt
     2281 cggcggccgg gcggatatag aggccgggtt ccaccgtcag tgtcatgccg ggcgcgagcg
     2341 tcgtccaggc gtcgccctcc ttgtattcac cggcatcgtg cacgtccagc cccagccagt
     2401 ggccggtgcg gtgcatatag aagcgcttgt aggcttccga ttcgagtgcg ccgtccaggc
     2461 tgccggtaag cagcttcaaa tccagcatgc cttgcgccag cacccgcagc gcggcctggt
     2521 gcggatccat gaagtggttg ccgggcttag tcgcggcgat cgccgccgcc tgcgctgcca
     2581 gcacgatctc ataggcgtcg cgctgcggcg cgctgaatcg accgttgacc gggaaggtgc
     2641 gggtgatgtc ggaggcatag ccctcgacct cgcagccggc gtcgatcagc actagttcgt
     2701 ggtcgttgag cagcttgtcg ttggcgacgt aatgcagcac gcaggcattg agaccgccgg
     2761 caacgatagg ggtgtaggcg tgcgcctcgg cgccgcggcg gcggaattcg taagtcagtt
     2821 cggcttccag ttcgtactcc gcgataccgg gacggcaggc gcgcatggcg cgtgcgtggc
     2881 cggcagagga gatcgcggcg gcgcgacgca tggcgtccag ttcatgggcg tccttggtca
     2941 gccgcatggc atcgagcggc acgcgcaggt cgcgaatttc cgccggtgcg cgcttgccgg
     3001 cacgcgcctg ggcacgtaca gcgttgagcg cagcggtgat gcgggcatcc cactccacgt
     3061 catggcccag cgaataccac agggcagggc ggtcggccag cagttcgccc aatcgggtgt
     3121 ccagttcggc aatggggtaa gccgcatcga aaccgaatgc ctcgcatgct ccctgcggcc
     3181 cgtggcggaa gccatcccag atttcgcgtt cctcgttctt gtcgcggcag aacaggatgg
     3241 attgcggccg ttttccaccg accagcacca gcaccgactc cggctcgggg aagccggtca
     3301 ggtaccagaa atagctgtcg aaacgatagg gatggtgcgc gtcgcggttg cgcagtttct
     3361 ccggcgcggt gggcaggacg gcgatgccgt cgccgagctg cgccagcagg cgcgcgcggc
     3421 gggaagaata gggttgatgg ctcatttcag ggtggcatcc agttcggcca ggcgttgcgg
     3481 ggtgccgaca tcggtccagc gcccgccgta gtattcgccg cccactttgc ccgaacgcat
     3541 ggcctcgcgc aacagcggtg ccagtttggc tttggctccg tgctggacac tggcaaaaag
     3601 tgcagggtgg taaacaccaa tgccgctgaa agtgagcatc tctcgacctt gctccgtgag
     3661 gcgatttccc cacagggaaa aatcgccgtt gccgttgtgg tcggggttat ccaccagcac
     3721 cagccaggcc agcaaccctt cacccaggct atgattcagt cgggcgaaat cgtagtcagt
     3781 aaaaatatca ccattcacca ccaggaacgg tgcttcgcct aacaaaggca gggcgttggc
     3841 gataccgccc gcggtttcca gcgcctcacc ctcgggcgag tactggatgg acacgttgaa
     3901 gcggctgccg ttccccagtg cggattcgat ctgtataccg agatgggcat ggttgatgac
     3961 gagttcgcgg aagccggcag cggccagacg ctcgatatgc caggcgatca gcggtttgcc
     4021 acccaccggc agcaaaggct tgggcatatg gtcggtcagc ggccgcatgc gctcgccgcg
     4081 gccggcggcc aggatcatgg ctttcatcaa aacgtgtatc caacctgtat ctgacggttt
     4141 tccagctggt cgagcaggtg cagcagcggc cgcagttcga tatagcgttc acaggcgcgg
     4201 cgcaggtaat ccatcaccag cggcaggtcc ttgaggtagc cctccttgcc gtcgcgatgg
     4261 tagaggcggg cgaaaatgcc gagcaccttc aggtggcgtt gtgcgcccat ccattcgaaa
     4321 tcgcggtaga aagtcgagat gtcgtccggc accggcaggc cggtttttct ggcctgctgc
     4381 caatagcgca ccacccagtc cagcacctgc gcctcttccc agcggatata ggcatccttg
     4441 agcagcgaca ccaggtcgta ggtgatggcg ccgtagacgg cgtcctggaa atccaggatg
     4501 ccggggtttg accccgtggc gtcgtcgcat accatcaggt tgcgcgagtg gtagtcgcga
     4561 tgcacgtaaa cctggccttg cgccagaata ttgcggttaa ggacgtcgaa cgtctgctgc
     4621 agcaccgctt gctgcttgtc atccagtgtc gtgcccaggt gtttgcctac ataccattcc
     4681 gggaacaggc gcatttcacg cgttagcagc gcttcgtcgt atggcggcag ggtgtcgggg
     4741 cgactcccct gctggatgcg caccagggct tccgtggcgt cgcgatagag tccgggggcg
     4801 gtctgttcgt tcagagcagt caggtaggtg gtattgccaa ggtcggtcag cagcaggaag
     4861 ccctgcgcca ggtcctggtc caggatgcgc ggcacgttga cgcctgcagc ggcgaacaat
     4921 tctgccacat gcaggaacgg ctcgcagttt tcatgctgcg gtggcgcatc catggcgatc
     4981 agggtgcgat ccggcagcgt tacccggaag tagcggcgga aactggcatc tgccgaggcg
     5041 ggggccagcg aaaaggattc accatgcagc gcgttgcgca gccagcgttg taattgttcc
     5101 agtctttgca cgttcaaacc aatcgattag tggttggggc atcgcattgt aaacaaaaga
     5161 gaatcgccta ataaaacgcg cttgggcgcg gctatcggca gtattttcgg attgcttaaa
     5221 gggcggattt gtgagatttt atcacctgaa aaatcaaacc ggcacttacc ttccctctcc
     5281 gttggaatca atgcaaacct ttcgcaacct tgcccatgct tttgtcttgc tgacggcaat
     5341 gtccgggcag acgcaggctg cggaaaacag tcctgccgag gcgcctgatg cggacgaaat
     5401 cacgatcgac gcccagcgcc tggaagtcta tcttgaccgg aaaatgagcg cgatcggcaa
     5461 tgccgaggtg catcgtagcg atcaggtact ctatggcgac cgtatcgact acgacatgat
     5521 caacgaggaa ttgcatgtct ccggcaatag ccgcatggtg cagggcggta ccgtggtcac
     5581 cggccccgac ttgcgcatga agctaggtga acgcgagggc gaaatgcagg atccggtatt
     5641 taccatgcgc aataccaaag cgctggtggg tgccagcacg cggtcaggca agaccgcgcg
     5701 gccgggagat atcagttttg cgcgtggcag cgccaaatcg gtaaccctgg acggcccgga
     5761 tcgcgagcgc tttacggatg cgcgctatac ctcttgtgag gaaggcgtgg acgactggta
     5821 cttgcgtgcc aaggatctgg agctcgacca ttccaccgat accgcaacag cgaaaaatgc
     5881 cagcatcgaa ttcaaggggg tccccatcct ctatacgccc tggatcgatt tccctttcag
     5941 cggccagcgc aaaagcggtt tcctttcacc tacgttcggt acgaccagtc tcaacggcgc
     6001 cgagttcgcc ttgccctact actggaatat cgcgcctgac atggatgcga ctattacgcc
     6061 gcgttacatg agcaagcgcg gcacgatgct ggggggggat ttccgctacc tgggggagca
     6121 gtattcaggc cgcgataacc tggactacct gcagcacgat gatttgaccg gccagacccg
     6181 ctggtttgca gacctggagc acaagcagaa cttcggcaat ggctggtcgg gcagctacga
     6241 tatcaagcgg gtttcggacg acaagtattt atccgacatg accaccaaca tcgtggccac
     6301 cagtacggtc aacctgccgc aacagggcat actcagctat ggcaccagca gcttgaacag
     6361 cagcttcaat ttcaacgcca tcgcacagga attccagacc ctggacaaca agtcctatcc
     6421 ctatttccgc ctgccggaat tcaacctgag cgccagcagg gactgggata acgtgacggg
     6481 caacatgtat gcgcaatggg tcagctttga gcaaagcaac gatgccccgg acaacgtcaa
     6541 gggcagccgt ttgaccagct accccagctt cagtctgccg ttgaacagct cctatggcta
     6601 tatcgtgccc aagctggggg cgaaatactg gacctacaat ctgagtggca acaccgtcat
     6661 ccccggcacc acggaaaact accaatccga cagcaccgcg ctgcccattt tcagcgtgga
     6721 cagcggggtg tatttcgacc gcaatt
//
LOCUS       DAOMFA010000090         6743 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_1428_length_6743_cov_4.795455, whole genome shotgun sequence.
ACCESSION   DAOMFA010000090 DAOMFA010000000
VERSION     DAOMFA010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 6743)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6743)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6743
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1428_length_6743_cov_4.795455"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..657
                     /locus_tag="PKW44_05725"
     CDS             <1..657
                     /locus_tag="PKW44_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011310733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 family protein"
                     /protein_id="HQR51118.1"
                     /translation="VEGLEKRAHKFLHGEAGDQSGHDATAAALNDPAIDLVAYEKEKV
                     RGAIRTDFVLSAEIIVIALGSAKDAALATQATVVAGIAFVMTVGVYGVVAGIVKLDDL
                     GLHLLRTGAENALSGLRRRIGSAILRAAPLLMKTLSVVGTAAMFMVGGGILLHGLPQA
                     HDLLHHAEQAMHGIPAVGGALGAVSPALLGGLAGVLAGALVLGLVSIGTFFISKMRRA
                     "
     gene            662..1699
                     /locus_tag="PKW44_05730"
     CDS             662..1699
                     /locus_tag="PKW44_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HQR51119.1"
                     /translation="MEYRRLGSSDLEASAVCLGTMTFGQQNTEAEAHAQLDYAFARGI
                     NFIDTAEMYPVPPRAETCTRTETIIGSWLSRQPRDKVILATKAAGPRRNMQWIRGGPA
                     SLDRTNLRQAVEGSLRRLQTDYIDLYQLHWPERNVPMFGQYQFDPAQETDALPIRAQL
                     EALAELVQEGKIRAVGVSNEQPWGVMEFLRIAKESGLPRIATIQNCYNLINRGLEFGM
                     AEIVYRENLSLLAYSPLAFGHLTGKYLPGGSADGRATLFKGFAQRYDKPNVQPACAAY
                     AELAARHGMSPATLALSFVMRRWFVASTIVGATSLAQLEENLDAWEKPLSEEVLKEIE
                     ALHLRYMNPAP"
     gene            complement(1789..3726)
                     /locus_tag="PKW44_05735"
     CDS             complement(1789..3726)
                     /locus_tag="PKW44_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HQR51120.1"
                     /translation="MIDRHYLELLAPAKNAEIGIEAVNHGADAVYIGGPAFGARANAE
                     NTVADIARLAAYAHKFNARVFVALNTILHDNELEAARQVTWQLYEAGADALIVQDMGL
                     LEMDLPPIQLHASTQTDIRTVEKAAFLDQVGFSQIVLARELDLSTIQSIRDNTSCNLE
                     FFVHGALCVAFSGQCFISHAHTGRSANRGECSQACRLPYTLQDAEGRILANDKHLLSM
                     KDNDQSANLRALIGAGISSFKIEGRYKEMPYVKNATAHYRLLLDEILEDLPGYDRASS
                     GRCTFTFTPQPEKTFNRSATDYFVNGRKGDIGAFDTPKFAGEEIGPVTKVGKDFVEVE
                     SGVELHNGDGISFFDSHKELVGLRVNVVDGKRLYPNEMPADLRRGMTLYRNRDHAFMR
                     LLEKPSAERRMQVAMRFAETASGFALTLTDEDGCTATAELPQEKQPAQQAERAEAALR
                     EGLGKLGNTDFAAGEITLDWTQPWFIPASGINALRREAIEQLQANRTAAFQRLPRRKP
                     VEPPAIYPEDTLSYLANVYNYKARAFYEKHGVRAIAAAYEANEELGEVSLMITKHCLR
                     YSYALCPKEAKGVLGVQGTITAEPMTLINGKEKLTLRFDCKKCEMHVMGKIRKPVLQM
                     AQPQVVEFHAQRLGRPLSTEV"
     gene            complement(3763..4005)
                     /locus_tag="PKW44_05740"
     CDS             complement(3763..4005)
                     /locus_tag="PKW44_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HQR51121.1"
                     /translation="MQPLEFSLKGEFVELNQLLKLNGLCDSGGAGKALVASGAVSVDG
                     QVELRKTCKIRTGQVVTVGDVAIRCLADTGQAPTKL"
     gene            4093..4368
                     /locus_tag="PKW44_05745"
     CDS             4093..4368
                     /locus_tag="PKW44_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET transporter"
                     /protein_id="HQR51122.1"
                     /translation="MTTEISNLVGYPAAFLTTIAFVPQAWKSWRTRDLSGILLPMYAL
                     FTVGVALWLAYGVMIGSIPIIVANAITFLLAFMVLWLKIVEAQSERD"
     gene            4451..4741
                     /locus_tag="PKW44_05750"
     CDS             4451..4741
                     /locus_tag="PKW44_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HQR51123.1"
                     /translation="MSKITVVHNPSQEKLNELGVSKWAIWEKEVSRFPIDFGSTECAY
                     VLEGEILVTPKGGEPVRIVAGDLVAFHAGLDSQWEVVKPLRKHYSYDFPNGF"
     gene            complement(4850..5467)
                     /locus_tag="PKW44_05755"
     CDS             complement(4850..5467)
                     /locus_tag="PKW44_05755"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQR51124.1"
                     /translation="MSANVFIIDDDEAVRDALALLCDSAGFIVETFDSAQAFLDVCGQ
                     YTEGCLVLDLNMPDMSGIELQENLVGRGVALPIIFLSAYGDVPTTARAMKAGAMDFLT
                     KPVAGGVLLTHIREALSLSARLRVQSETQRSILENVAKLTEREKEVLVRVATGASNKE
                     IAQDMGISYRTVEIHRANAMHKVGAANLLELARIVEVAKLHSRTP"
     gene            complement(5464..>6743)
                     /locus_tag="PKW44_05760"
     CDS             complement(5464..>6743)
                     /locus_tag="PKW44_05760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQR51125.1"
                     /translation="PSPEGPAKNALLENRDLRVKIASGEELPIKLSLNPLPYSDTVYT
                     LATLHDLSWLRKTENALRLTEATYQSAIETSQDSFLLLDMDGLILEGNDEFCQRAGCS
                     REDLKGMNVRQLIFPQTITRLGTPYAVEMLTDKQNSMFKAKLRYKNDTSWYAEIKKTY
                     WPMQGGRIFVFIRDLTDQLVTMRKLRENRRKMLGLLKKQVASQTAAAIAHELNQPLNA
                     ISTYSTAALTILKSENPSSKKLETAIAGSAEQALRAGRVVKELLAFLTFGEVPQENLD
                     LNQAVLDALEYVDYDGFAGFAAVLEFDPDLPPVRGNRIQIQKVLTNLLFNSIEAAHEA
                     GISSTALTIRLKTFKSAPMAQFTIRDNGPGISSEVAQHLFEPFVTSKSNGTGFGVYVS
                     RTLIESMGGRLWADFGDGSGAAFHFTLPLAAPL"
BASE COUNT         1257 a         2068 c         2042 g         1376 t
ORIGIN      
        1 gtcgaaggct tagagaagcg ggcgcacaag ttcctgcatg gcgaggccgg ggaccagtcc
       61 gggcacgatg caaccgccgc cgcgttgaac gaccccgcca tcgaccttgt ggcgtatgaa
      121 aaggaaaagg tccggggcgc gatccgcacc gatttcgtcc tttccgccga aatcatcgtc
      181 attgccctcg gcagcgccaa agatgctgcg ttggcgaccc aggccacggt cgttgccggt
      241 atcgccttcg tcatgaccgt cggcgtctac ggcgtggtcg ccgggatcgt caagctggat
      301 gaccttggcc tgcacctgct caggactggt gccgaaaatg cactttccgg cctcaggcga
      361 cgaatcggca gcgccattct gcgtgccgca ccgctgctga tgaagaccct ctccgtcgtc
      421 ggcactgccg ccatgttcat ggtcggcggc ggcatcctgc tgcacggcct gccgcaggcg
      481 catgacctgc tgcaccacgc ggaacaggcc atgcacggca tccctgccgt cggcggcgcg
      541 ctgggagcgg tcagccccgc cctgctgggc ggtctggctg gcgtgctggc cggcgcgctc
      601 gtgctggggc tggtcagcat cggaacgttt tttatctcga agatgcggag ggcctgattc
      661 catggaatac cgtcgtttgg gaagcagcga tctcgaagct tccgccgtct gcctgggcac
      721 catgaccttc ggccagcaga acaccgaggc cgaagcccac gcccagctgg attacgcctt
      781 cgcgcgcggc atcaacttca tcgacacggc ggaaatgtac ccggtaccgc cacgcgcgga
      841 gacctgcacc cgcaccgaga ccatcatcgg cagctggctg agccggcagc cgcgcgacaa
      901 ggtcatcctc gccaccaagg ccgccggccc tcgccgcaac atgcagtgga tacgcggcgg
      961 gccagcctcg ctggatcgca ccaacctgcg ccaggcggtg gaaggttcgc tcaggcgttt
     1021 gcaaaccgat tacatcgacc tctaccagct gcactggccg gagcgcaacg tgcccatgtt
     1081 cggccagtac cagttcgatc cagcgcagga aaccgacgcc ctgcccatcc gcgcgcaact
     1141 cgaggcgctg gccgaactgg tgcaggaagg gaaaatccgc gccgtcggcg tttccaacga
     1201 gcagccctgg ggcgtcatgg aattcctgcg catcgcgaag gaatccggcc tgccgcgcat
     1261 cgccaccatc cagaactgct acaacctcat caaccgcggc ttagagttcg gcatggccga
     1321 gatcgtctac cgcgagaacc taagcctgct cgcctactcg ccgctcgcct ttggccacct
     1381 caccggcaaa tacctgcccg gcggcagcgc cgacggccgc gccaccctgt tcaagggctt
     1441 cgcccagcgc tacgacaagc ccaacgtgca acccgcctgc gccgcctatg cggaactggc
     1501 cgcaaggcac ggcatgtcgc cggccaccct ggcgctctcc ttcgtcatgc gccgctggtt
     1561 cgtcgccagc accatcgtcg gcgcgaccag cctggcacag ttggaggaga acctggatgc
     1621 ctgggaaaaa ccgctgtcag aggaagtgct gaaggaaatc gaagcgctgc atctgcgcta
     1681 catgaacccg gcgccttgag gctggccttt gccttcggcc aacagcgcgc attcataatt
     1741 gataagttcg agcctcactc ggggagccac cccaggctcg aaccagggtc agacttcggt
     1801 cgatagcggg cgcccaagcc gttgcgcgtg aaactccacc acctgaggct gggccatctg
     1861 tagcaccggt ttgcggattt ttcccatcac gtgcatctcg cacttcttgc agtcgaagcg
     1921 cagggtcagc ttttccttgc cgttgatgag cgtcatcggt tcggcggtga tggtgccctg
     1981 cacgccaagc acgcccttgg cttcctttgg gcagagggca tagctgtagc gcaggcagtg
     2041 cttggtgatc atgagcgaaa cctcgcccag ctcctcgttg gcctcgtagg ccgccgcgat
     2101 ggccctgacc ccgtgctttt cgtagaacgc ccgcgccttg tagttgtaga cgttggcgag
     2161 gtagctcagg gtatcttccg gatagattgc gggcggctcc accggcttgc ggcgcggcag
     2221 gcgttggaag gctgcggtgc ggttggcctg cagttgttcg atggcctcgc ggcgcagggc
     2281 attgatgcca gatgcgggga tgaaccaggg ctgcgtccag tccagcgtga tctcgccggc
     2341 agcgaaatcg gtgttgccga gctttcccaa cccttcgcgc agggccgctt cggcacgctc
     2401 cgcctgctgc gctggctgtt tttcctgcgg cagttccgcc gtggcggtgc agccgtcttc
     2461 gtcggtgagg gtcagggcga atccgcttgc ggtttcggca aagcgcatcg ccacttgcat
     2521 gcggcgctcg gcggaaggct tttccagcag gcgcatgaaa gcatggtcgc ggttgcggta
     2581 gagcgtcatg ccgcgcctta gatccgccgg catttcgttg ggataaaggc gcttgccgtc
     2641 cacaacgttg acgcgcaggc cgaccagttc cttgtggctg tcgaaaaagc tgatgccgtc
     2701 gccgttgtgc agctcgacgc cgctttccac ctccacgaaa tccttgccca ccttggtgac
     2761 cgggccgatt tcctcgccgg cgaacttggg ggtgtcgaag gcgccgatgt cgcccttgcg
     2821 gccgttgacg aagtaatccg tggcactgcg gttgaaggtc ttttccggct gcggggtgaa
     2881 ggtaaacgtg cagcggccgg aggaagcgcg gtcgtagccc ggcaggtcct ccaggatttc
     2941 gtccagcagc aggcggtaat gtgcggtggc gttcttgacg tagggcatct ccttgtagcg
     3001 cccctcgatc ttgaacgagc tgatgccggc gccgatcagc gcacgcaggt tggcgctctg
     3061 gtcgttatcc ttcatggaga gcaggtgttt gtcattcgcc aggatgcgcc cctctgcatc
     3121 ctgcagcgta taaggcaggc ggcaagcctg cgaacactcg ccgcggttgg cgctgcgccc
     3181 ggtgtgggcg tggctgatga agcactggcc gctgaaggcc acgcacagcg cgccatgcac
     3241 gaaaaactcc agattacatg aagtattatc tctaatgctt tgaatcgttg acaaatcaag
     3301 ttcgcgtgcg agcacaatct gggagaagcc gacctggtcg aggaaggccg ccttttccac
     3361 cgtgcggatg tcggtctggg tactggcatg caactggatc ggcggtaaat ccatctccag
     3421 cagccccatg tcctggacga tcagcgcatc ggcgccggct tcgtagagct gccaggtgac
     3481 ctggcgtgcg gcttccagct cgttgtcgtg caggatagta ttgagcgcga cgaacaccct
     3541 ggcattgaac ttgtgggcgt atgcagccaa ccgggcgatg tcggccaccg tattttcggc
     3601 attggcgcgc gcgccgaacg ctgggccgcc gatgtaaacg gcatccgccc cgtgattgac
     3661 ggcctcgatg ccgatttcgg catttttggc gggggcgagc aactcgaggt aatggcggtc
     3721 aatcatggga aaagctgtta aatcaaaagg gtgcgcacta tatcacaact tcgtgggtgc
     3781 ctgccccgta tcggccagac agcggattgc cacatcgccc acggtcacca cctgccctgt
     3841 ccgtatcttg caggttttac gcaattccac ctggccgtcg accgaaaccg cgccgctggc
     3901 gaccagcgcc ttgcccgcac cgccgctgtc gcacaggccg ttgagtttaa gaagctggtt
     3961 gagctcgacg aactcgcctt tgaggctgaa ttccagtggt tgcatgcgta ctccaaagta
     4021 tcggtgaaat ggacttgggg cggcgacgcc attaaacttg cggctctcga cctgccaggc
     4081 gccaatactg acatgaccac cgaaataagc aacctggtcg gctacccggc cgccttcctg
     4141 accaccatcg catttgtgcc gcaggcctgg aaatcctggc gcacgcgcga cctgtccggc
     4201 atcttgctgc ccatgtacgc gctgttcacc gtcggcgtgg cgctgtggct ggcctacggc
     4261 gtcatgatcg gcagcatccc tatcatcgtc gccaatgcca tcaccttcct gctggcgttc
     4321 atggtgttgt ggctgaagat tgtggaagcc cagtccgagc gcgattgatt ggcaaaagcc
     4381 agccaaaaca agtatagtcg cgcccgacga aacaacgtct tttcaggatt cattgattaa
     4441 ggatagattc atgagcaaaa ttaccgtggt acacaatccg agccaggaaa agctgaacga
     4501 actgggcgtc tcgaagtggg cgatctggga aaaggaagtc tccaggttcc cgatcgattt
     4561 cggttccacc gagtgcgcct acgtactgga aggcgaaatc ctggtcaccc ccaagggcgg
     4621 cgaaccggtg cgcatcgtcg ccggcgacct ggtggccttc cacgccggcc tggacagcca
     4681 gtgggaagtg gtcaagccac tgcgcaagca ctacagctac gatttcccca acgggtttta
     4741 attcgtagca ggcgtttcag caagcaggcc gggagcaggg aaaacccggc ctgcgcgcac
     4801 gaaaaaatgc caaccgatcg cggttggcat tttttcgccc tgactcaggt caaggcgtgc
     4861 ggctatgcaa cttcgccact tccacaatac gtgcgagttc cagcaggttg gcggcgccga
     4921 ctttgtgcat ggcgttcgcg cgatggattt ctaccgtccg gtagctgatt cccatatcct
     4981 gtgcgatctc cttgtttgac gccccggtgg cgacgcgcac caggacctct ttttcccgct
     5041 cggtaagttt ggcaacgttt tcaagaatgg accgctgcgt ttcggactga actcgcagcc
     5101 tggcggagag gctcagtgcc tcccggatgt gcgtcagcaa tacgccgccc gccaccggct
     5161 tggtcagaaa atccatggcg cccgccttca tggcccgcgc cgtggtagga acatccccat
     5221 aggcgctgag gaaaatgatg gggagcgcca cccctcgccc gacaagattt tcctgcagtt
     5281 caatcccgct catgtccggc atgttcagat cgagcaccag acagccctct gtgtattggc
     5341 cgcaaacatc cagaaacgcc tgtgcgctgt cgaatgtttc cacgatgaac cctgcgctgt
     5401 cgcagagcag cgccagcgcg tcgcggaccg cctcgtcgtc gtcgataatg aatacgtttg
     5461 cgctcaaagg ggggcggcga gaggcaaggt gaaatgaaag gccgcgccgg agccgtcacc
     5521 aaaatcggcc cacaggcgcc cgcccataga ctcgatcagg gtgcgactga cataaacccc
     5581 gaagccggtg ccgttggact tgctcgtgac aaacggttcg aacaggtgct gggcgacttc
     5641 gctgctgatg ccggggccat tgtcgcggat ggtgaactgc gccatgggcg cacttttgaa
     5701 agttttcagt ctgatggtaa gggcggtact ggaaatgccg gcttcgtgcg ccgcctcgat
     5761 gctgttgaac aacagattgg tgagcacttt ctggatctgg atgcgattgc cgcgcaccgg
     5821 gggcaagtcg gggtcgaact caagtactgc agcaaatccg gcaaagccat cgtaatccac
     5881 gtattccagg gcatcgagca ctgcctgatt gagatccagg ttttcctggg gtacttctcc
     5941 aaaggtgaga aaggcgagca attccttgac cacgcggccc gcccgcaggg cctgctccgc
     6001 actgccggcg atcgccgttt ccagcttttt cgagctgggg ttttccgact tcaggatggt
     6061 aagtgcagcc gtactgtagg tggagatggc gttgagcggc tggttcaact catgcgcaat
     6121 ggccgccgcc gtctgcgatg cgacctgctt cttgagcaga ccgagcattt ttctgcgatt
     6181 ttcccgcagc ttgcgcatgg tgacgagttg atcggtaaga tcgcggatga acacaaaaat
     6241 gcgcccgccc tgcatcggcc agtaggtttt tttgatttcc gcataccatg aagtgtcatt
     6301 cttgtagcgc aacttggcct tgaacatcga attctgctta tccgtcagca tttccaccgc
     6361 atatggcgta cccagcctgg tgatggtctg agggaagatc agctgccgca cgttcatgcc
     6421 tttcagatct tcccggctgc atcctgccct ctggcagaat tcatcattgc cttccagaat
     6481 caatccgtcc atgtccagca ataaaaaact gtcctgcgag gtttcgattg cagactgata
     6541 ggtggcttcc gtaagcctca gggcattttc ggtttttctg agccagctca gatcatgcag
     6601 cgtggccagc gtgtacacgg tgtcggaata cgggagaggg ttgaggctga gcttgatcgg
     6661 cagctcttcc ccggatgcga tctttacgcg caggtcacgg ttttcgagca aggcattttt
     6721 agcggggcct tcgggagatg gtt
//
LOCUS       DAOMFA010000091         6558 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_1489_length_6558_cov_4.717669, whole genome shotgun sequence.
ACCESSION   DAOMFA010000091 DAOMFA010000000
VERSION     DAOMFA010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 6558)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6558)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6558
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1489_length_6558_cov_4.717669"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="PKW44_05765"
     CDS             <1..228
                     /locus_tag="PKW44_05765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HQR51126.1"
                     /translation="ILRENYQSSNQQITAKTEITTAPTNPEHQTQVATSQTQSPGSHE
                     QELKELKQLYAQGLITQEVYVERQKAILGSK"
     assembly_gap    298..307
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(380..844)
                     /gene="trmL"
                     /locus_tag="PKW44_05770"
     CDS             complement(380..844)
                     /gene="trmL"
                     /locus_tag="PKW44_05770"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HQR51127.1"
                     /translation="MFDIVLFQPEIPPNTGNIIRLCANTGARLHLVKPLGFTLEDKQL
                     KRAGLDYHEFASLLVHENWQDCLDALADRRLFAASTRGSTPYDRVAFQAGDVFVFGPE
                     TRGLPPEILETFSPEQRIRLPMLPHSRSLNLSNSAAVIVYEAWRQNGFGGGA"
     gene            complement(859..1518)
                     /locus_tag="PKW44_05775"
     CDS             complement(859..1518)
                     /locus_tag="PKW44_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HQR51128.1"
                     /translation="MPQQCLLCGAASGATALCGGCHADLPWQDQAHACPRCGLPAPVG
                     AVCGACLKHPPQFDATLAVLHYRFPADAMLRRYKYHGLLAVADLLGGLLAARAADIPR
                     PDLLVPMPLYRQRLHERGFNQAAEIARVAAGKLGLALDLQACQRTRPTPPQAGLKLRE
                     RVKNMRGAFACNSSLQGKHVALVDDIMTSGASLNELARTVKDAGAARVECWVVARTPL
                     D"
     gene            1611..2036
                     /locus_tag="PKW44_05780"
     CDS             1611..2036
                     /locus_tag="PKW44_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR51129.1"
                     /translation="MPVKTVTAAEQRRAAQIFNICSCVAIFLLPLIIPALLWIAASIF
                     VHASIAHHPNQRVRDFLYPGGYRFYGLVGSLVVVLNFSPKMSSWVPGVVNLGPLHLPM
                     FWVWVWVVSLLVVLPLGIRDIVRANRENWQDMTVEVEHA"
     gene            2029..3018
                     /gene="bioB"
                     /locus_tag="PKW44_05785"
     CDS             2029..3018
                     /gene="bioB"
                     /locus_tag="PKW44_05785"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HQR51130.1"
                     /translation="MLETTIEFVKPAVAKPEAERWSVAQIAELFELPFSDLLYRAQSV
                     HRQHFDPNAVQISTLLSIKTGGCSEDCGYCPQSARYHTGVENEDLLALDDVVAAARSA
                     RDSGASRFCMGAAWRGPKQRDLEPVLKMIEEVKALGLETCATLGMLKEGQAEQLRDAG
                     LDYYNHNLDTAPEFYGEVISTRTYQDRLETLDRVRDAGIHVCCGGIVGMGESRNQRAG
                     LIAQLANMEQPPESVPINLLTQVEGTPLDGTEELDPFEFVRTIAAARITMPQSYVRLS
                     AGRERMSDELQALCFLAGANSIFYGEKLLTTSNPDVSRDQALFAKLGIRPENH"
     gene            3029..4201
                     /gene="bioF"
                     /locus_tag="PKW44_05790"
     CDS             3029..4201
                     /gene="bioF"
                     /locus_tag="PKW44_05790"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HQR51131.1"
                     /translation="MPADSPWAGLAADLEARAAEGLLRQRRLADGPQQPLLQVDGREL
                     LSFCSNDYLGLASHPALVAALQQAAGEAGVGSGASHLITGHHRLHHELEIALARFVDL
                     PRALLFSTGYMANLGVVSALVGRGDAVFADRLHHASLNDAAVLSRAELHRYPHNNLST
                     LEKRLAGSTAKRKLVVADAVFSMDGDLAPVPQLLELCERHDAWLLLDDAHGFGVLGQH
                     GRGVLEHFGLHSPRIIYMATLGKAAGVFGAFVAGEPELVETLIQRARTYIYTTAMPPA
                     LAAALLVALPLIEQESWRRTQLQTLIETLKGRLKLQRWRLEPSPTPIQPLVIGSNEEA
                     LAVSERLREQGTLVPAIRPPTVPNGTARLRISLSAAHSVADVERLVAALHAAEARA"
     gene            4198..4962
                     /gene="bioH"
                     /locus_tag="PKW44_05795"
     CDS             4198..4962
                     /gene="bioH"
                     /locus_tag="PKW44_05795"
                     /EC_number="3.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HQR51132.1"
                     /translation="MKPHVETVGSGSDLVLIHGWGMHGGIWDGVRDALSQQHQLHVVD
                     LPGMGHSPLCAPYHLQQLAQLVAEAIPPQADVCGWSLGGQIAMQLALDDPKKVRRLLL
                     VGSTPRFVNAADWRHGIDAEVFRQFARNVATDYEETMTRFLSLQAFGGEASRNQMREL
                     RERFVQRPLPDPEALRQALDVLLHTDLRQQLARLHLPVMLVHGERDTLAPVAVAHWMA
                     QHIPRAQLRVIAGASHAPFLSHPDSFLDAVTSFLEH"
     gene            4962..5840
                     /gene="bioC"
                     /locus_tag="PKW44_05800"
     CDS             4962..5840
                     /gene="bioC"
                     /locus_tag="PKW44_05800"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HQR51133.1"
                     /translation="MQEDVYTLDKARVRASFDRAAHSYDAAAVLQKEVRERMSARLDL
                     VRIAPRNVLDLGAGTGHAARALAARYPGSHVVALDIALGMLRLANAGQPWWRRFTQKQ
                     QSTLCADMESLPLAAASMDLAWSNLAIQWSNDLDRVFAEMARVLRPEGLLTFSTFGPD
                     TLKELRAATTADPQHVHVSRFIDMHDIGDALVRAGFSAPVLDVERFVLTYDDALALMR
                     DLKAIGAHNATQGRRRGLEGKGFLQGLTGRYERFREQGKLPATYEVIYGHAWKAEEKT
                     IAASAGASIIRLHPRK"
     gene            6101..>6558
                     /locus_tag="PKW44_05805"
     CDS             6101..>6558
                     /locus_tag="PKW44_05805"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HQR51134.1"
                     /translation="MDSVTVAGLLQKFSLFGELTPEQLAEVAREAQYQFAARGQILFN
                     RGDSAHGMFLLLDGQVKLAVSSPQGAEKVIGIIGAGESFGEAIIFLDRPFFPISAQTT
                     TDSKLLMIPKHVIFGLLERDSSIALKMLAGLSMRNHQLVQDIESVALLTC"
BASE COUNT         1280 a         2061 c         2030 g         1177 t
ORIGIN      
        1 attttacggg agaattacca atcaagtaac caacagatca cagcaaaaac agaaatcaca
       61 actgcaccga caaatccaga gcaccagact caagtagcta cgtctcaaac acaatcccct
      121 ggtagtcatg aacaagagtt aaaagaattg aaacaactat atgcccaagg attaatcacc
      181 caagaagttt acgtggaacg tcagaaggcg attttaggat caaaataagc tggaaatatt
      241 actgagagac aaaccacatc catcacgagt cggcaaggta ggaacttcct tgccactnnn
      301 nnnnnnnatg caccgtggcc gggtgcggga aatttcattt accagaagat tcaacataat
      361 taggccggct tggaccacat tacgcaccac ccccaaaccc attttgccgc caggcttcat
      421 agacgatcac cgccgccgag ttggataaat tgaggctgcg gctgtgcggc agcatcggca
      481 gccggatgcg ctgttccgga ctgaaagttt ccaatatctc cggcggcagc ccccgcgttt
      541 ccgggccgaa cacgaacaca tcccccgcct gaaaagccac ccggtcgtag ggcgtactgc
      601 ccctggtgct ggccgcgaac agccggcggt cagccagcgc gtccaggcag tcctgccagt
      661 tctcatgcac cagcagcgag gcgaattcgt ggtagtccag ccccgccctt ttcaattgct
      721 tgtcctccag cgtaaacccc agcggcttga ccaggtgcag gcgcgcgccg gtgttggcgc
      781 acaggcggat gatgttgccg gtgttgggcg ggatttccgg ctggaagagg acgatgtcga
      841 acatgctcat ttttagattc aatccagcgg cgtacgggcg accacccagc actcgacgcg
      901 cgctgcgccg gcatctttga cggtgcgcgc cagttcgttg aggctggcgc cgctggtcat
      961 gatgtcgtcc accagcgcca cgtgcttgcc ttgcagactg gaattgcagg cgaacgcccc
     1021 gcgcatgttt ttgacgcgct cgcgcaattt cagtccggcc tgcggcgggg tcggccgggt
     1081 gcgctggcag gcttgcaggt cgagcgcgag gcccaacttg ccggcggcga cgcgggcgat
     1141 ttcggcggcc tggttgaagc cgcgctcatg tagccgctgc cggtacaaag gcatgggtac
     1201 cagcaggtcc gggcgcggaa tgtccgccgc acgcgctgcc agcaggccac ccagcaagtc
     1261 cgccaccgcc agcaggccgt ggtatttgta gcgtcgcagc atggcgtcgg ccggaaatcg
     1321 ataatgcagc acggcgaggg tggcgtcgaa ctgcggcggg tgcttgaggc aggcgccgca
     1381 cactgcgccg accggcgccg gcaggccgca gcgcgggcag gcgtgcgcct gatcctgcca
     1441 gggcaggtcg gcatggcagc cgccgcacaa ggctgttgct ccagaagcgg cgccgcatag
     1501 caggcattgc tgcggcaaca gaaactgctt aatttttgag ccaatgtcaa tcaattgccg
     1561 ccatccggtt tgacagaata taatgtgccc ttccaacagg gggttccatt atgccagtga
     1621 aaaccgtgac tgccgccgag cagcggcgcg ccgcgcaaat cttcaacatc tgttcgtgtg
     1681 tagcgatttt cctgctgccg ctgattatcc ccgccctgct atggattgcc gcatcgattt
     1741 ttgtccacgc gtcgatcgcg caccacccca accagcgcgt gcgcgacttc ctctaccccg
     1801 gcggctaccg cttttatggc ctggtcggct cgctggtggt ggtgctcaat ttcagcccga
     1861 agatgtcgtc gtgggtgccc ggtgtggtca acctggggcc gctgcatctc cccatgttct
     1921 gggtatgggt gtgggtggtc agcctgctgg tagtcctgcc gctcggcatc cgcgacatcg
     1981 tgcgtgccaa cagggagaac tggcaggaca tgaccgtgga ggtggagcat gcttgagacg
     2041 accatcgaat tcgtgaagcc ggctgtcgcc aaacccgaag ccgagcgctg gtcggtggcg
     2101 cagatcgccg aactgttcga actgccgttc agcgacctgc tgtaccgcgc gcagagcgtg
     2161 catcgccagc atttcgaccc caacgcggtg cagatcagca ccctgctgtc gatcaagacc
     2221 ggcggctgct ccgaggattg tggctattgc ccgcagtcgg cgcgctatca caccggcgtg
     2281 gaaaacgaag acctgctggc gctggacgac gtggtggccg cggcgcgttc cgccagggat
     2341 agcggcgcct cgcgcttctg catgggcgct gcctggcgcg gccccaagca gcgcgatctc
     2401 gaaccggttc tgaaaatgat cgaggaagtg aaggcgctgg ggctggaaac ctgcgccacg
     2461 ctgggcatgc tgaaggaagg ccaggccgag cagttgcgcg acgccggcct cgactactac
     2521 aaccacaacc tggataccgc gccggaattc tacggcgagg tgatttccac gcgcacctac
     2581 caggaccgcc tggaaacgct ggatcgcgtg cgcgacgccg gcatccacgt ctgctgcggc
     2641 ggtatcgtcg gcatgggcga atcacgcaac cagcgcgccg gcctcatcgc ccagctcgcc
     2701 aacatggaac agccgccgga aagcgtgccg atcaacctgc tcacccaggt cgaaggcacg
     2761 ccgctggatg gcaccgagga actcgaccca ttcgagtttg tgcgcaccat cgccgccgca
     2821 cgcatcacca tgccgcaatc ctatgtccgg ctttccgccg ggcgcgagcg catgtcggac
     2881 gaattgcagg cgctatgctt cctggctggg gcaaactcga ttttctacgg cgagaagctg
     2941 ctgaccacca gcaaccctga cgtcagtcgc gaccaggcgc tgttcgccaa actgggcatc
     3001 cgccccgaaa accactaggg caacaggcat gcccgccgac tccccatggg ccgggctggc
     3061 ggccgatctc gaagcgcgtg ccgcggaagg gctgttgcgc cagcgccgtc tggcagatgg
     3121 gccacagcaa ccattgctgc aggtggatgg ccgcgagttg ctgtccttct gcagcaacga
     3181 ctatctcggc ttggccagcc acccggcgct ggtggccgcg ctgcaacaag cggccggcga
     3241 ggccggcgtc ggcagtggcg cctcgcacct gattaccggc caccatcgcc tgcatcacga
     3301 actggaaatt gcgctggcgc gctttgtcga cctgccgcgc gcgctgctgt tttccaccgg
     3361 ctatatggcc aatctcggcg tggtatcggc tctggtaggc cgcggcgacg cagtgttcgc
     3421 cgaccgcctc caccacgcct cgctcaacga cgcggcagtg ctatcgcgcg ccgagctgca
     3481 ccgctatccg cacaacaacc tctccacgct ggaaaagcga ctggccggca gcacggccaa
     3541 gcgcaagctg gtagtggctg acgccgtatt cagcatggac ggcgacctcg ccccggtgcc
     3601 gcaactgctg gagctgtgcg aacgccacga cgcctggctg ctgctggacg atgcgcacgg
     3661 cttcggcgtg ctgggccaac atggtcgcgg cgtgctggaa catttcggcc tgcacagccc
     3721 ccgcatcatt tacatggcca cgctgggcaa ggctgccggc gtgttcggcg ccttcgtcgc
     3781 cggagagccg gaactggtgg aaaccctgat tcagcgcgcc cgcacctaca tctacaccac
     3841 cgccatgccg cccgcgctgg ccgccgcgct gctggtggcg ctgccgctga tcgaacagga
     3901 aagctggcgg cgcacgcagt tgcaaaccct gatcgaaacc ttgaaaggcc gcctgaaact
     3961 gcagcgctgg cggctggaac cttcgccgac gccgatccag ccgctcgtca tcggcagcaa
     4021 cgaagaagct ctggcggtga gcgaacgctt gcgtgagcaa ggcaccctag tgcccgccat
     4081 ccgcccgccg accgtcccga atggcacggc gcggctgcgc atctcgcttt ccgccgcgca
     4141 cagcgtggcc gatgtcgagc gcctggtcgc ggcattgcat gcggcggagg cgcgcgcatg
     4201 aagccgcatg tcgaaaccgt cggcagcggc agcgatctgg tgctgatcca cggctggggc
     4261 atgcacgggg gcatctggga tggcgtgcgc gatgcgctgt cgcagcaaca ccagctgcat
     4321 gtggtggact tgccgggcat gggacacagc ccgctgtgcg cgccttatca cctgcagcaa
     4381 ctggcacagc tggtcgccga agcgatcccg ccgcaagccg atgtctgcgg ctggtcgctg
     4441 ggtggccaga tagcgatgca gctggcgctg gatgatccga aaaaggtgcg ccggctgctg
     4501 ctggtcggca gcacccctcg cttcgtcaat gcagcggact ggcgacacgg tatcgacgcc
     4561 gaagtgttcc ggcagtttgc ccggaacgtg gcgacggatt acgaggaaac catgacgcgc
     4621 ttcctcagcc tgcaagcctt cggcggagaa gcctcgcgca accagatgcg cgagctgcgc
     4681 gaacgcttcg tccagcgtcc actgcccgat ccggaggctt tacgccaggc cttggatgtc
     4741 ctgctgcaca ctgacttgcg ccagcaactt gcccgactgc atctaccggt catgctggtg
     4801 catggggagc gcgacacgct ggcgccggtg gcagtcgcac actggatggc gcagcacatt
     4861 ccgcgggcgc aactgcgcgt gatcgcaggc gcttcgcacg ccccgtttct ttcccatcca
     4921 gacagttttc tggatgcagt cacgagtttt ctggagcact gatgcaagaa gacgtttaca
     4981 ccctggacaa ggcgcgcgta cgcgcctcct tcgaccgcgc ggcgcacagc tacgacgctg
     5041 cagcggtact gcagaaggaa gtgcgcgagc gcatgtcggc gcggttggat ctggtcagaa
     5101 tcgccccgcg caatgtgctc gacctcggcg ccggcactgg ccacgccgca cgcgcgctgg
     5161 cggcacgcta tccgggcagc cacgtggttg cgctggacat cgcgctcggc atgctacggc
     5221 tggcaaacgc cggccagccc tggtggcggc ggttcacaca aaagcagcaa agcacgctgt
     5281 gcgcagacat ggaaagcctg ccgctggccg ccgccagcat ggatttggcg tggtccaacc
     5341 tcgcgatcca gtggagcaac gaccttgacc gcgtgtttgc cgaaatggcg cgcgttctac
     5401 gccccgaggg gttgctgacg ttcagcactt tcggcccgga tacgctcaag gaactgcgcg
     5461 cggcgacgac agccgacccg cagcatgttc atgtcagccg cttcatcgac atgcacgaca
     5521 tcggcgacgc cctggtgcgc gccgggtttt ccgcaccggt actggacgtg gagcgcttcg
     5581 tgctgaccta cgacgacgcg ctcgccctga tgcgcgacct caaggccatc ggcgcgcaca
     5641 acgcaacgca aggccgccgg cgcgggctgg aaggcaaggg ctttctgcag gggctgacgg
     5701 ggcgctacga gcgctttcgc gagcagggaa aattaccggc gacttacgag gtgatttacg
     5761 gccacgcctg gaaagcagaa gaaaaaacca ttgcagcatc ggccggcgcc agcattatcc
     5821 ggctgcaccc aagaaaatag gctggcccga tcccggaaca acctggacgg ttgccgacgg
     5881 ctattcaacg gggctggtta gcacccggaa aagcttggga ggtgcggatt ttccagaggg
     5941 gtggctagct tttgcatcaa gccgcacgca tttggttggt ttagccgatt ggctaaaaga
     6001 actagttaca ttcctaaaaa acctgtcctt aagaagaatg gacatatttg cactcgaaga
     6061 ataccctcca cgagcatatc gagaggagga gggtgtggac atggacagtg tcaccgttgc
     6121 cggcttgttg cagaaattca gcttgttcgg agagttaact cccgaacagc ttgcggaggt
     6181 tgcaagggaa gcgcaatacc agtttgcagc acgcggacaa attctgttca accgtggtga
     6241 ttcggcacat ggcatgtttc tgttgctgga tggacaggta aaactggcag tctcttcacc
     6301 gcaaggagca gaaaaagtta ttggcatcat cggggcggga gaaagcttcg gtgaagccat
     6361 tatctttctc gatcggcctt tcttccccat ttccgcgcaa acaacaacag attcgaagtt
     6421 gctgatgatc cccaagcacg tcatttttgg cttgctggaa cgcgacagtt ccatagcgct
     6481 gaaaatgctg gcggggcttt cgatgcgcaa ccaccagttg gtgcaggata tagaatccgt
     6541 cgccctgctc acatgtag
//
LOCUS       DAOMFA010000092         6554 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_1490_length_6554_cov_4.407755, whole genome shotgun sequence.
ACCESSION   DAOMFA010000092 DAOMFA010000000
VERSION     DAOMFA010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 6554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6554
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1490_length_6554_cov_4.407755"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..955
                     /gene="ilvA"
                     /locus_tag="PKW44_05810"
     CDS             <1..955
                     /gene="ilvA"
                     /locus_tag="PKW44_05810"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HQR51135.1"
                     /translation="AAYVKRLRPEIRVIGVEAVDADAMTRSLAAGKRVVLEQVGLFAD
                     GAAVKQVGEETFRLCQQYVDEMILVDNDAICAAIKDVFEDTRSILEPAGALAVAGAKA
                     YVKQHKLHGKTLVAIGSGANMNFDRLRFVAERAEIGEQREALLAVTIPEKPGAFRSFC
                     KLLGNRNITEFNYRFADAKQAHIFVGVTTHQRGESQRLVSMLERHELPALDLTDNEMG
                     KLHLRHLVGGHAPQVQNEQVYRFEFPERPGALMNFLEHMSAGWNISLFHYRNHGADYG
                     RVLVGIQVPPEEKQAFRSFLDKLGYAWWDESANPAYKLFLG"
     gene            complement(961..1416)
                     /locus_tag="PKW44_05815"
     CDS             complement(961..1416)
                     /locus_tag="PKW44_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HQR51136.1"
                     /translation="MRYWLMKSEPGDVSIDDLASFPDQSVDWYGVRNYQARNFMRDQM
                     KVGDGVLFYHSNCAEPGIAGIAEVSKPAYPDRTQFDSKHRYFDPKATPENPRWFNVDV
                     KLVRKTRLLSLKELRDTPELASLRILQRGNRLSITPVDPREWEFILSIL"
     gene            complement(1527..1706)
                     /gene="ssrS"
                     /locus_tag="PKW44_05820"
     ncRNA           complement(1527..1706)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="PKW44_05820"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(1722..2027)
                     /locus_tag="PKW44_05825"
     CDS             complement(1722..2027)
                     /locus_tag="PKW44_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013440984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HQR51137.1"
                     /translation="MSESKGIDVSIMGREFRVSCTDDERAALMQAVSYLDRKMCEIRD
                     SGKVASVERIAIMAALNIAHELLTTRTGGFDIGDFKRRIDLMQEQIDQAVDEQNALF"
     gene            complement(2020..2202)
                     /locus_tag="PKW44_05830"
     CDS             complement(2020..2202)
                     /locus_tag="PKW44_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011313234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR51138.1"
                     /translation="MEAKLTQLIALYQQLRNENLGLRQDLAQAQDDVRQLKENMILAG
                     DRLEALIESLPGESDE"
     regulatory      2325..2533
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            2655..4511
                     /locus_tag="PKW44_05835"
     CDS             2655..4511
                     /locus_tag="PKW44_05835"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQR51139.1"
                     /translation="MKRTILAGIACVLVSLSAHAQESIYSDEVVVTANRVPQSRENVL
                     ADVSVIEREEIDRAGQSTLAELLRSQPGVEIDSYGGPGSLSNVRLRGTSSQSIVVLVD
                     GMRVSSATTGTTAFNQIPPEQIERIEIVRGPVSSLYGADAVGGVIQIFTRQGDGKNRV
                     SGYAGTGSYNTHQFGGGVSGGNESTRFALNLGKINTDGISSFHTHQGYDADDDGFSNI
                     SYSGHLSHVLTEGHDVALQAYGSNGHNQVDGNDFPAYADLRQYSYALTSNNRFTDYWL
                     SHIKLGQSMDGNKGVGLYYDTWVRTYQRQYSWQNDLTLPLGMLTLAYDRLEQHVESNT
                     DYSRTHRSDNGWLANYLLEQGPHAFRAGLRTDDDSQFGRHNTGNIGYGYRFDQYWRAA
                     GNYGTAFRAPTFNDLYWPYQNYGFGYSYQGNPDLKPETSRNKEISLVYDQGHHRISAT
                     AFHNKVDDLIIGFQGIAADTPANVGSATIKGLTLTYEGWFGTYHLRANGDVQSPKDDD
                     TGKVLPRRARQYASLVASKTWGELEVGTEMNASGQRYNDTDNTIKLDGYALVNLIANY
                     TLNADWSLNARFNNVFDREYALATTAFSFAPDAPAYNTPGANLFVGIRYQPK"
     gene            4561..5127
                     /locus_tag="PKW44_05840"
     CDS             4561..5127
                     /locus_tag="PKW44_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR51140.1"
                     /translation="MTPDNRKQFGIAAILALLVATTHGHHFASTLHLPPATWAVFFMA
                     GFYVRRARLFAALLAEVVLIDCVAVTAGGVSAFCLSPAYGFLLPAYGALWLAGRWYAT
                     RYRFVWNTLPALGASLLSGVVLAEIFSSGGFYFFSGRFADTNLAEFGNRLLMFFPYSL
                     QSFAFWTGVAIVVHVVMALSQSGRQHHA"
     gene            5141..5746
                     /gene="cobO"
                     /locus_tag="PKW44_05845"
     CDS             5141..5746
                     /gene="cobO"
                     /locus_tag="PKW44_05845"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019899848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HQR51141.1"
                     /translation="MTGPDASARHADRMARKKAVIDQAIARADAEKGLLLVLTGNGKG
                     KSSSAFGMAVRALGHGMKVGVAQFIKGRTDTGEEAFFRGCKNIEWHVLGEGFTWDTQD
                     RERDIQKAQEGWEIARKMLADPSFKLVILDELTYTMNYGYLDPEQVLRDISARPAMQH
                     VVVTGRAASAELIAAADTVTELVDVKHAWRAGIKAQPGIDL"
     gene            5910..5985
                     /locus_tag="PKW44_05850"
     tRNA            5910..5985
                     /locus_tag="PKW44_05850"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5943..5945,aa:Phe,seq:gaa)
     gene            complement(6298..>6554)
                     /locus_tag="PKW44_05855"
     CDS             complement(6298..>6554)
                     /locus_tag="PKW44_05855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR51142.1"
                     /translation="EEFDHGDLVRCCTWLYLAEMLGSDLTKDDYHAIHEDGSPYDDDV
                     GGPIFADGRDGVRLEPISADQQATARQNAAEMFRVIRDDE"
BASE COUNT         1405 a         1968 c         1831 g         1350 t
ORIGIN      
        1 cgccgcctac gtcaagcgcc tgcgcccgga aatcagggtg atcggcgtcg aggcagtgga
       61 tgccgacgcc atgacgcgct cgctggcggc gggcaagcgc gtggtgctgg agcaggtagg
      121 cctgttcgcc gacggcgctg cggtcaagca ggtcggcgag gaaactttcc gcctgtgcca
      181 gcaatacgtg gacgagatga tcctggtaga caacgacgcc atttgcgccg ccatcaagga
      241 tgtattcgag gacacgcgct ccatcctgga acccgcgggc gcacttgccg tcgcgggagc
      301 taaagcgtat gtaaagcagc acaagctgca cggaaaaacg ctggtcgcca tcggcagcgg
      361 cgccaacatg aatttcgacc gtctgcgctt tgtcgccgaa cgtgctgaga ttggcgaaca
      421 gcgcgaggca ttattggcag tgacgatccc cgaaaaaccg ggtgcattca ggagtttctg
      481 caaactactc ggcaaccgca acatcacgga attcaactat cgttttgccg atgcgaagca
      541 ggcgcacatt ttcgtcgggg tcactacgca ccagcggggg gaatcgcaac gcctggtgtc
      601 aatgctggaa cgacacgaac tgccggcgct ggatctgacc gacaacgaaa tgggcaagtt
      661 gcatttgcgg catcttgtcg gcggccacgc gccgcaggtg cagaacgaac aggtgtaccg
      721 cttcgaattc ccggaacgcc cgggcgcatt gatgaatttc ctggagcaca tgagcgccgg
      781 ctggaacatc agcctgttcc actaccgcaa ccacggggcc gattacggac gggtgctggt
      841 aggcatacag gtgcctcccg aggaaaaaca ggcattccgc tccttcctcg acaagctagg
      901 ctatgcttgg tgggatgaat cagccaaccc tgcctacaag ttatttctgg gctaacaagc
      961 ctagaggata gaaaggatga actcccactc gcgcgggtcc accggcgtga tcgacaaccg
     1021 gttaccgcgc tgcaggatgc gcagactggc cagttcaggc gtatcgcgca actccttcaa
     1081 actcaacaac cgcgtcttgc gcaccagctt cacatccacg ttgaaccagc gcgggttttc
     1141 cggcgtggcc ttggggtcga aatagcgatg cttggaatcg aactgggtgc ggtcgggata
     1201 ggccggcttg ctgacttcgg cgatgccggc gatgcccggc tcggcgcaat tggagtggta
     1261 gaacagaacg ccatcgccga ccttcatttg atcacgcata aaattgcgcg cctgatagtt
     1321 gcgcacgcca taccagtcca cgctctggtc aggaaaggaa gcgaggtcat caatcgacac
     1381 atcgccgggt tcggacttca tcagccagta acgcatagac tattcaatca gttcgggttg
     1441 cattcggatg aagacgaagc gggggtgaga ggttcaggct atagggcaac gatttgttcg
     1501 ttcgaatgca acagaagcca aatgagaagt tccccgcaag cgaagccaga ccggcatcct
     1561 gaacctgctg aacaggtggt cgcagcggga gcgcatcagg cacgcccgct gacggggcac
     1621 gcacaacagc actcgtttga gccactaccg agatgcttga atattggttc aaggaatcta
     1681 tggtctgaca aacaccgcag ggaacaaacc ggaaaaactt tttagaacag cgcgttttgc
     1741 tcgtctaccg cctgatcaat ctgttcctgc atgagatcga ttctacgctt gaagtcaccg
     1801 atgtcaaatc ccccggtgcg ggtcgtcagc agctcgtggg caatattcag cgcggccatg
     1861 atggcgatgc gctcgacact ggccaccttg ccgctgtcac gaatctcgca cattttacgg
     1921 tcgagatagg acaccgcctg catcagcgcg gcgcgctcgt cgtcggtaca ggaaacgcgg
     1981 aactcgcgcc ccatgatgct gacatcgatc cccttggatt cactcatcgg attcccctgg
     2041 caggctttcg atcaaggctt ccagacggtc gccggcaagg atcatgtttt ctttcagttg
     2101 ccgcacatcg tcctgcgctt gggccagatc ctggcgcagt ccgagatttt cgttgcgcaa
     2161 ctgctggtag agggcaatga gttgtgtgag ctttgcttcc aaagctttta aatccgcatc
     2221 catggcgcaa ctataagtgg aaaatctctt gccggtcaac cgctaacgat aaattatgaa
     2281 agtattacaa catgttactg gtgtccgccg gaatttattg ctaaaatttt cccacgttgt
     2341 caggtgccga aattgcgatt ccgcgatttt ggtgaaacgg gaaacaggtg cgttttcttt
     2401 aaagccaagc ctgtgctgcc cccgcaacgg taagcaagtg tgggttcacc atcatgccac
     2461 tggaatttat cccgggaagg cggtgaatca agacttgtga gcccggagac cggcctgatg
     2521 accacgttgg aaataccgcg gggtgacggt gttcggtccg gcaaagcatc cgctttccct
     2581 actgatccct ttattcattg cggtttccat attttgtttc ggcatgcggg gtcgcatgcc
     2641 caaaggggaa gctcatgaaa agaactattc tggctggcat cgcttgcgtg ctggtgtcct
     2701 tatccgccca cgctcaggag tccatttact cggatgaggt ggtggtcacc gccaatcgcg
     2761 tgccgcaatc ccgcgaaaac gtgctggcgg acgtatccgt catcgagcgc gaagaaatcg
     2821 accgcgccgg acaatctacg ctggccgagt tgttgcgcag ccagccgggt gtcgagatcg
     2881 actcctacgg cggtccggga agcctgtcca acgtgcgttt gcggggcacc agctcgcaat
     2941 ccatagtggt gctggtagac gggatgcgcg tcagctccgc cactaccggt accaccgcgt
     3001 tcaaccagat tcccccggaa cagatcgaac gcatcgagat cgtacgcggc ccggtttcca
     3061 gcctctatgg cgcggacgcg gtcggtggcg tgatccagat attcactcgc cagggagacg
     3121 gaaaaaaccg ggtcagcggc tatgccggca ccggcagcta caacacccac cagttcgggg
     3181 gcggcgttag cgggggcaac gaaagcaccc gctttgcgct caatctcggc aaaatcaaca
     3241 ccgatggcat ttcctcgttc catacccacc agggttacga cgctgatgac gacggcttca
     3301 gcaatatctc ctattccggc catctcagcc atgtcctgac cgaaggccat gacgttgcgc
     3361 tacaggccta tggcagcaac ggccacaacc aggtcgacgg caatgatttt cctgcctatg
     3421 cggacttgcg gcagtatagt tatgcgctga ccagcaacaa ccgcttcacg gactactggc
     3481 tgagccacat caagctgggc caaagcatgg atggaaacaa gggcgtcggc ctctactatg
     3541 acacctgggt caggacttac cagcgccagt actcctggca gaacgacctt accctgccgc
     3601 tgggtatgct cacgctggcc tatgaccggc tggaacagca tgtcgagagc aatacggact
     3661 actccaggac gcatcgcagc gacaacggct ggctggccaa ttacctgctg gagcaggggc
     3721 cacacgcctt ccgcgccggc ctgcgcaccg acgacgattc tcagttcggc cggcacaaca
     3781 ccggcaacat cggttatggc taccgtttcg accagtactg gcgcgccgca ggtaactacg
     3841 gcaccgcttt ccgtgcccca accttcaatg atctgtattg gccctaccag aattacggct
     3901 tcggctactc ctaccagggc aatcccgatc tcaagccgga aacttcacgc aacaaggaaa
     3961 tctctctggt gtacgaccaa gggcatcatc gcatctccgc caccgccttc cacaacaagg
     4021 tggacgacct catcatcggc ttccagggga tcgccgccga tacccccgcc aacgtcggat
     4081 cggccaccat caagggcctt acgctgactt acgaaggctg gtttggcacc tatcatctgc
     4141 gcgccaacgg cgatgtgcaa agcccaaagg atgacgatac cggcaaggtg ttgccacgcc
     4201 gggcgcgcca gtatgcctct ctggtggcga gcaaaacttg gggtgaactg gaagtcggca
     4261 ccgaaatgaa tgccagcgga cagcgttaca acgatacgga caacaccatc aagctcgacg
     4321 gctatgcctt ggtcaacctc atcgccaatt acacgctgaa tgcagactgg tcgctcaacg
     4381 caaggttcaa caacgtgttt gaccgggaat acgcgctcgc caccaccgca ttcagctttg
     4441 ccccggatgc acctgcgtac aatacacccg gcgccaatct gtttgtcggc attcgctacc
     4501 aaccgaaata atcgcgcgat gggttacaat tctgtcaaag tgaatttaag gagtacatcc
     4561 atgaccccgg acaaccgcaa gcaattcggc attgccgcca tcctcgccct gctggtcgcc
     4621 accacccacg gccaccattt cgccagcacc ctgcacctgc cgcctgctac ctgggcggta
     4681 ttcttcatgg ccggcttcta tgtccgccgt gccaggctgt tcgcagcact gctggccgag
     4741 gtggtcttga ttgactgcgt tgccgtcact gcgggcggcg tcagcgcctt ctgcctcagc
     4801 cccgcctacg gcttcctgct gccggcttac ggcgcgctgt ggctggccgg acgctggtac
     4861 gccacgcgct accgctttgt atggaacacg ctgcccgcgc tcggcgccag tttgctgtcc
     4921 ggcgtggtgc tggccgaaat attcagcagc ggcggcttct atttcttctc cggccgcttc
     4981 gccgatacca acctagctga attcggcaac cgcctgctga tgtttttccc ctacagcctg
     5041 caatcctttg ccttctggac tggcgtagcg atagtggtgc acgttgtcat ggccctatcg
     5101 caatccggca gacagcatca cgcctgaacc ttccaaactg gtgaccggtc ccgatgcaag
     5161 cgcgcgccat gccgaccgca tggcgcgtaa aaaagctgtc attgaccagg ccatcgcccg
     5221 cgccgatgcc gaaaaggggc tactgctggt gctgaccggc aacggcaagg gcaagtcttc
     5281 ctctgccttc ggcatggcgg tgcgtgcact tgggcacggc atgaaagtcg gcgtcgcaca
     5341 gttcatcaag gggcgcacgg ataccgggga ggaggccttt ttccgcggct gtaaaaacat
     5401 cgaatggcat gtcctgggcg aggggttcac ttgggacacc caggaccgcg aacgcgatat
     5461 ccagaaagcg caagaaggct gggagatcgc gcgcaagatg cttgccgacc ccagcttcaa
     5521 actggtcata ctggacgaac tgacctacac catgaactat ggctacctgg atccggaaca
     5581 ggtgttgcgc gacatcagcg cccgcccggc catgcagcac gtagtggtta ccggccgcgc
     5641 cgcttctgcg gaactcattg ccgcagcaga cacagtcacg gaactggttg atgtaaaaca
     5701 tgcatggcgg gcaggaatca aggcgcagcc cggcatcgac ttgtaacccc actgcctcga
     5761 gataaatact tgccgaagga gcgcccgcag tgagtttgcc ctcctgccgg tccgctgccg
     5821 gcatacaaca gttggaaatt tcgttttgca ggtgcggcga ttttgctata atcgcgcctt
     5881 cttgacgctt ggcaacaggc aaaagacacg gccaggtagc tcagttggta gagcagcgga
     5941 ttgaaaatcc gcgtgtcggc agttcgattc tgcccctggc caccaaaatt caaaccccaa
     6001 ccactctcgg ttggggtttt ttattgcctg tttgcgccaa ctcccgcgtg ttctcgcggg
     6061 ttccttcgga gtcttgcgga cttcgccttc gccaccgcta ggcaggttcg gcgcccggtt
     6121 ctctctctat tggccctttc tctctgcggg cgggactttg tgttttttcc cgaagtccgc
     6181 aaaggtcacg acccaccccc tataaagatc aatgggttac gagttgtcga atcgactggt
     6241 ttgttttaca gattggtagg cgagtaattc gtcacccacc aaccccaggc gttgctacta
     6301 ctcgtcgtcc ctgatcacac ggaacatctc cgctgcgttt tgccgtgcag ttgcctgttg
     6361 atctgcactg atcggttcaa gcctgacacc atcgcggccg tctgcaaaga tggggccacc
     6421 gacgtcatcg tcgtaagggg acccatcttc gtggatagcg tggtagtcgt ccttggtcag
     6481 atcgctacca agcatttctg cgaggtagag ccatgtgcaa caacgaacca gatcaccatg
     6541 gtcaaactct tcaa
//
LOCUS       DAOMFA010000093         6347 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_1566_length_6347_cov_4.667514, whole genome shotgun sequence.
ACCESSION   DAOMFA010000093 DAOMFA010000000
VERSION     DAOMFA010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 6347)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6347)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6347
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1566_length_6347_cov_4.667514"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..368
                     /locus_tag="PKW44_05860"
     CDS             <1..368
                     /locus_tag="PKW44_05860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR51143.1"
                     /translation="ATPLRAFAGPMQRTPASSPSLVAAFDQYLAPPALLYLRGPQEKL
                     SAWKSRLDQMWLPGAIVIALSEAAPGLPQALQHPLRDEVNAWLCRGVECLPVVESWGA
                     LLDKLQVGGMNSTQQGRPV"
     gene            544..873
                     /locus_tag="PKW44_05865"
     CDS             544..873
                     /locus_tag="PKW44_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HQR51144.1"
                     /translation="MKHLINLFAAAGLVLVAGQASAAVDNAAGQALAQKSGCLLCHAV
                     DKKVMGPSYKDIAAKYKGQADAEAKLITKVSKGGSGTWGPIPMPANSPKVSDADIKTL
                     VQWVLAQ"
     gene            complement(919..2277)
                     /locus_tag="PKW44_05870"
     CDS             complement(919..2277)
                     /locus_tag="PKW44_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HQR51145.1"
                     /translation="MTQTSRRSYWTNRWTFILATAGSAIGLGNIWKFPYMAGVNGGSA
                     FVLIFLACIALVGIPLMMCEIMLGRRAQRNPVDGMQALAQEAGASGLWRAIGIMGLVA
                     GLLILGFYSVIAGWVLDYVGRAASGAFHGMDGKMAQEQFGAFLAAPLELILWHTLFIA
                     MTMGVVARGVNAGLEKANKILMPALFAILLVLLGYSMAQGDFTRSAQFMFTPDFSRIT
                     PGAVLSALGHAFFSLSLGMGAVMVYGSYLQPHISIARTTLYVAIADTAVGLLVGLAIF
                     ALVFSNGLEPAAGPGLIFQTLPIAFGSMPAGGLMATLFFLLVAFAAWTSAISLVEPAI
                     AWITENTRITRKRAALLIALGDWLLGVAVALSFNAWREVKLAFGFNIFDTLDKLTTNV
                     MLPLGGLLMALFAGWVMRTHHVREELGLAVRPYAAWRFTIRFVSPLAIIAIFLYLFGL
                     VK"
     gene            complement(2284..3606)
                     /locus_tag="PKW44_05875"
     CDS             complement(2284..3606)
                     /locus_tag="PKW44_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HQR51146.1"
                     /translation="MQAIETLIAILSGWVWGPPMLALLVGTGIYLTVLLKGVQFRALP
                     LAFRLIFHKDHGHAGDISHFAALMTALAATVGIGNIVGVATAITLGGPGAVFWMWMTG
                     LVGMATKYSEAVLAIKYREKGEHGMRGGPMYYLAKGAGLPWLGTLFAVFTALAAFGIG
                     NMTQANATAKILVTTFHVDTLVTGLVLMVLTGLVILGGIRSIGRFTSLLVPFMIVGYV
                     AAALLVLVLNAAEIPSALGLIFQHAFSPAAASGGFAGATIAAAMRFGIARGVFSNESG
                     LGSAPIAAAAARTNDPVKQALVSMTQTFIDTLVVCTMTALVILTATSWTHGVGAGELT
                     SASMSETLGKAGAIIVALSTALFAYSTLIGWNYYGEKAVEYLFGERSIRIYRIFFIAA
                     VIVGATTSLEFVWNFSDLMNGMMAIPNLIGLLLLSKVIKAETERYFKA"
     gene            3706..4266
                     /locus_tag="PKW44_05880"
     CDS             3706..4266
                     /locus_tag="PKW44_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018987642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HQR51147.1"
                     /translation="MIFQLEFMRMRYPGYILALCFALGGGLAHAEGTAASQQSDVVAQ
                     PATQWPNVAQEVLLNALSLTGVQYKYGGKSPETGFDCSGFVRYVFQQAASLTLPHGAR
                     AISQLGQAIPLDQLQPGDLVFFNTLKSAFSHVGIYMGEGRFIHAPSSGGDIRIVDMND
                     SYWAKRFNGARRLPVSARQLAEVSAE"
     gene            complement(4205..5146)
                     /locus_tag="PKW44_05885"
     CDS             complement(4205..5146)
                     /locus_tag="PKW44_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HQR51148.1"
                     /translation="MNIRKHSFGFLITLLLTSCAIHPSIEQRPVTSPPAEPSTAKPEA
                     KPTPPKVALVLGGGAVRGFAHVGVIKVLEAQGIVPDMVIGTSAGSVVGALYSAGYSGF
                     ELQKIAFKLDEDSVGDWAIPGRGFIKGEALQNYINKAVRGQPIEKLKKPFAAVATDLQ
                     SGEQMVFRRGNTGQAVRASSSVPGVFQPVTINGREYVDGGLTSPVPVRVAREMGADVV
                     IAIDIAGKPKYRKVEGLTDVLLQTFAIMGQSISSRELLDADVVIRPETGSFGSADFDK
                     KHEAILEGEKAAQALLPLIRQKLQQAAERKPANGAPR"
     gene            5283..5600
                     /locus_tag="PKW44_05890"
     CDS             5283..5600
                     /locus_tag="PKW44_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HQR51149.1"
                     /translation="MSRIALVTLLFPVTVFAADTDFTLTIRNHAFQPAQLEVPAGKKI
                     KLLVVNQDATPEEFESYPLNREKVIAGNSSASMYIGPLEPGRYPFFGEFHESTAQGVI
                     IAK"
     gene            5617..>6347
                     /locus_tag="PKW44_05895"
     CDS             5617..>6347
                     /locus_tag="PKW44_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HQR51150.1"
                     /translation="MFGTAIIVFREVLEAAIIIGIVAAATRDIPGRKRWIAAGLLTGL
                     AGSGLVAASTDTIAAMASGIGQELFNAIVLGIAVLMLAWHNIWMASHGAALAFDARSV
                     GRNIREGRSECSILLVVVGLAVLREGSETVLFLYGIAASDGTGVSAMLLGGLIGVAAG
                     IVVGYALYAGLLKIPIRWFFAATAALVLLLAAGMASQAARFLIQADLLPSLAAPLWDT
                     SVLLPEHSVAGMLLHSLTGYDARPAG"
BASE COUNT         1288 a         1860 c         1932 g         1267 t
ORIGIN      
        1 ccgcgacgcc gctgcgcgcc ttcgccggcc ctatgcagcg caccccggcc tctagtccga
       61 gccttgtggc tgcgtttgac caatacctgg cgccccccgc cttgctatat ctgagggggc
      121 cgcaggaaaa gctatctgcc tggaaatcaa ggctagacca gatgtggctg cccggggcga
      181 tcgtgatcgc cctgtcggaa gccgccccag gcttgccaca ggcgctgcag cacccgttgc
      241 gggacgaagt caacgcctgg ttgtgccggg gcgttgaatg tctgccagta gttgaaagct
      301 ggggtgcatt gctggataaa ttgcaggttg gcgggatgaa tagcacacag caggggcggc
      361 ccgtgtaacc cgctcatgaa tcgtaagggc tttgcaagtg ccggcaaact ggataagatt
      421 gtgcgaagct gtccttagag gctgcgtgac aagcccttat gccgatgaat caaggatcaa
      481 gatcattttc gattctgata gactgggttt tatctcatta atcacgttta ggaggagtta
      541 ttcatgaaac atctgatcaa tctgtttgcc gccgccggtc tggttctggt tgccggccaa
      601 gcttccgcag ccgtcgacaa tgcggctggt caggcgctgg cgcaaaagag cggctgcctg
      661 ctgtgccacg ccgtggacaa gaaggtgatg gggccgtcct acaaggatat cgccgctaag
      721 tacaaaggcc aggctgatgc cgaagccaag ctgatcacca aggtgtccaa gggtggtagc
      781 ggcacttggg gtccgatccc gatgccggcc aacagcccca aggttagcga tgccgacatc
      841 aagaccctgg ttcagtgggt gctcgcacag taaccgggcg atttgaacca gacgaaggcc
      901 ggcgtgagcc ggcctttttt atttcactag tccgaacagg tataggaaga tggcgatgat
      961 ggccagcgga gaaacgaaac ggatggtgaa gcgccatgcc gcataaggcc tgaccgcgag
     1021 tcccagttct tcgcgcacat gatgggttcg catcacccag ccggcgaaca gcgccatcag
     1081 gagcccaccc aggggcagca tgacgttggt ggtaagtttg tccagggtgt cgaaaatgtt
     1141 gaagccgaac gccagtttca cctcccgcca ggcattgaat gacagggcta ccgcgacgcc
     1201 gagcagccag tctccgagcg cgatcagcaa ggctgcgcgc ttgcgtgtga tccgtgtgtt
     1261 ttcggtgatc cacgcgattg ctggctccac cagcgagatg gccgaagtcc aggcggcgaa
     1321 agcgaccagt aggaaaaata gggttgccat caggccgccg gctggcatgc tgccgaaggc
     1381 gatgggcagg gtctggaaaa tcagacctgg cccggcggca ggctccaagc cgttggagaa
     1441 caccagcgcg aagatggcaa gccctaccaa caaccccact gcggtgtcgg cgatggccac
     1501 gtacaaggtg gtgcgggcga tggagatgtg cggctgtaaa taggagccat acaccatcac
     1561 cgcgcccatg cctagactga gcgagaagaa ggcatggccc agtgctgaca gtactgcgcc
     1621 tggcgtgatg cgggagaaat ccggggtgaa catgaattgg gcggagcggg tgaagtcgcc
     1681 ctgcgccata ctgtagccga gcagcaccag caggatggcg aacagcgccg gcatcagaat
     1741 cttgttggct ttctccagtc cggcgttgac accgcgagcc accacgccca tggtcatggc
     1801 gatgaacagc gtatgccaca gtatcagctc cagaggcgcg gcgagaaagg cgccgaactg
     1861 ctcctgcgcc atctttccgt ccatgccgtg gaatgcaccg ctggcggcac ggcctacgta
     1921 atccagcacc cagccggcga tgacgctgta gaagccgaga atcagcaggc cggcgaccag
     1981 tcccatgatg ccgatggctc tccacagccc ggacgccccg gcttcctgtg ccagtgcctg
     2041 catgccgtcc accgggttgc gctgggcgcg tcgccccagc atgatttcgc acatcatcaa
     2101 gggaatgccg accagcgcga tacaggcgag gaaaatcagg acgaaggcgc taccgccgtt
     2161 gactccggcc atgtagggga atttccagat gttgcccagc ccgattgccg atccggccgt
     2221 agccaggatg aaggtccacc ggttggtcca gtagctacgg cgcgaggtct gggtcatggg
     2281 agtttatgcc ttgaaatatc gttcagtctc tgccttgatg accttggata gtaacagcag
     2341 gccgatcagg ttgggaatcg ccatcatgcc gttcatcagg tcggagaaat tccagacgaa
     2401 ttccagactg gtggtcgcgc cgacaatcac cgccgcgatg aagaagatac ggtagatgcg
     2461 gatggagcgt tcgccaaaca ggtattcaac cgctttttcg ccatagtagt tccagccgat
     2521 cagggtggag tatgcgaata gcgcggttga cagggcgacg atgatggccc cggccttgcc
     2581 aagcgtttcg gacatgctgg cgctggtgag ctcaccagcg ccaacgccat gcgtccatga
     2641 ggtggcagtc aggatgacca aggcggtcat ggtgcagacc acaagggtgt cgatgaaggt
     2701 ctgcgtcatg ctgaccagtg cctgtttcac cggatcgttg gtgcgcgccg ccgctgccgc
     2761 gatgggggcc gagccgaggc cggattcgtt ggaaaatacg ccgcgcgcga tgccgaaacg
     2821 catggcggcg gcaatggtgg cgccggcgaa accgccgctg gccgcggcag gactgaaggc
     2881 atgttggaag atcaggccta gcgcactcgg gatttcggcg gcgttgagca ccagcaccaa
     2941 tagtgcggcg gcgacgtagc cgacgatcat gaacggcacg agtaacgagg tgaagcggcc
     3001 tatggagcgg atgccaccca gtatcaccag ccccgtcagc accatcagta ccagcccggt
     3061 gacgagggtg tcgacgtgga atgtagtcac gaggattttt gcggtggcgt tggcctgcgt
     3121 catattgccg atgccgaacg cggccagcgc ggtgaatacg gcgaacaggg tgcccagcca
     3181 tggcaggccg gcgcccttgg ccagatagta cattggcccg ccgcgcatgc cgtgctcgcc
     3241 tttttcgcgg tatttgatcg ccagcactgc ttcggaatac tttgtggcca tgccgaccag
     3301 cccggtcatc cacatccaga ataccgcacc ggggccgccc aaggtgattg cggtggccac
     3361 gccgacgatg ttgccgatgc cgaccgtggc cgccagtgcc gtcatcagcg cggcaaaatg
     3421 gctgatatcg ccggcgtgtc catgatcctt gtggaagatt aggcggaatg ccagcggcag
     3481 ggcacgaaac tgcacccctt ttaacagtac cgtcagatag attccggttc ccaccagcag
     3541 ggccagcatc ggcggccccc agacccagcc ggacaatatg gcgatcagcg tttctatggc
     3601 ttgcatgttt tttcctctca gtcacgcgat tatatgcaga aacgcggtct acgccagcca
     3661 tgaaatccga gcgcgcagaa aatgcgtcgt aatgtttaca acatcatgat ttttcaatta
     3721 gaattcatgc gcatgcgcta ccccggatac attcttgcac tgtgtttcgc ccttggcggt
     3781 ggccttgccc atgccgaagg cacggctgcc agtcagcaaa gtgatgttgt tgcccagcct
     3841 gccacgcagt ggccgaacgt cgctcaggaa gtgctgctca acgcgctgag tctgaccggg
     3901 gtgcagtaca aatatggtgg aaagtcgccg gaaaccggct tcgattgcag cggtttcgtc
     3961 aggtacgtat tccagcaggc cgccagtctg acactgccgc acggcgcgcg cgccatcagc
     4021 cagttggggc aggccattcc gctcgatcag ttgcagcccg gtgatctggt gttcttcaat
     4081 actttgaaaa gtgcgttctc ccacgtcggg atctatatgg gagaaggtcg tttcatccat
     4141 gcgccgtcca gcggcggcga tatccgtatc gtggatatga acgatagcta ctgggctaag
     4201 cgctttaacg gggcgcgccg tttgccggtt tccgctcggc agcttgctga agtttctgcc
     4261 gaataagcgg cagcagggcc tgcgccgctt tctccccttc caggatcgcc tcgtgctttt
     4321 tgtcaaagtc ggctgagcca aagctgcctg tctccggccg tatcaccacg tcagcatcca
     4381 gcagttcacg gctgctgatg gattgcccca tgatggcgaa ggtttgcagc aggacatcgg
     4441 tcaggccttc aaccttgcga tatttgggct tgccggcgat gtctatcgcg atcactacgt
     4501 ccgcgcccat ttctcgcgct actctgaccg gtaccggact ggtcaggccg ccgtccacat
     4561 attcccgccc gttgatggtc accggctgaa atacgcccgg tacgctgctg gaggcacgca
     4621 ccgcctggcc ggtgttgccg cgccggaaca ccatctgctc accgctctgc aagtcggtgg
     4681 cgacagcagc gaatggtttt ttcaacttct ctatgggttg gccgcgcacc gccttgttga
     4741 tgtagttctg tagagcctcg cccttgatga acccacggcc ggggatggcc cagtcgccca
     4801 cgctatcctc gtccagtttg aacgcgattt tctgcaactc gaaaccgctg taaccggccg
     4861 agtaaagcgc ccccaccacg ctgcctgcac tggtaccaat caccatgtcc ggcacgatgc
     4921 cctgcgcctc cagcaccttg atcacgccca catgggcaaa cccgcgcact gccccgccgc
     4981 ccagcaccag tgccactttt ggaggtgttg gcttggcttc cggcttagcc gttgaaggct
     5041 cggccggtgg cgacgtaact gggcgctgct ctattgacgg atggatggcg caacttgtca
     5101 gcagcagtgt gatgagaaat ccgaagctat gtttgcgtat gttcataacg ggtgagcata
     5161 aattctgtta gtggagagcg ccagcagaaa aaagttgcaa gcacggggca aattagaaaa
     5221 gccttgcata tataaatgcg aatcgttatc attaataaaa aatgaacgag aggaatccaa
     5281 tcgtgagccg tatagcactt gtcaccttgc tgtttccagt cactgtattt gccgctgata
     5341 ctgatttcac gctgaccatc aggaatcatg cgttccagcc ggcgcaactc gaagttccgg
     5401 caggaaaaaa gatcaagttg ctggtcgtga accaggacgc gacgccggaa gaattcgaaa
     5461 gctacccgct caaccgcgag aaagtaatcg ccggcaacag ctccgctagc atgtacatcg
     5521 gaccgctgga accgggccgt tacccgttct ttggcgagtt ccacgagagc accgcccagg
     5581 gcgtcatcat cgccaaataa tagatcggag ccatccatgt tcggaactgc cattatcgtt
     5641 tttcgcgaag tgctggaagc cgccatcatt atcggcatcg ttgccgccgc cacacgcgat
     5701 attccaggcc gcaaacgctg gatcgcggct ggccttctga cggggctggc cggctctggc
     5761 ctggtcgccg catccaccga caccattgcc gccatggcca gcggcatcgg ccaggaactg
     5821 ttcaacgcca tcgtactcgg cattgcggta ctgatgctgg cctggcacaa catctggatg
     5881 gcctcgcacg gggcggcact ggcattcgat gcgcgcagcg tcggaagaaa tatccgcgag
     5941 ggacgcagcg aatgctccat cttgctggtg gtcgtcgggc tggcggtgct gcgggagggc
     6001 tcggaaaccg tgctgttcct gtatggcatc gccgcatcgg atggcaccgg agtatccgcc
     6061 atgctgctgg gaggcctgat cggtgtggca gccggcatcg tggtcggtta tgcgctatat
     6121 gccggcctgc tgaagatccc gatacgctgg tttttcgccg ccaccgctgc tctggtgctg
     6181 ctgctggctg ccggcatggc ctcgcaggct gcacgctttc tgattcaggc cgatctgctg
     6241 cctagcctgg ccgcgccatt gtgggacacc tcggtattgc tgcccgagca ttccgttgca
     6301 gggatgctgc tgcacagtct gaccggttac gacgcgcgtc cggccgg
//
LOCUS       DAOMFA010000094         6325 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_1573_length_6325_cov_5.088198, whole genome shotgun sequence.
ACCESSION   DAOMFA010000094 DAOMFA010000000
VERSION     DAOMFA010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 6325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6325
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1573_length_6325_cov_5.088198"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(184..1197)
                     /locus_tag="PKW44_05900"
     CDS             complement(184..1197)
                     /locus_tag="PKW44_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-6-deoxy-delta-3,4-glucoseen reductase"
                     /protein_id="HQR51151.1"
                     /translation="MSFQVTIQPSGHQFSIQDDETVLSAALDAGFNLPYGCRNGACGA
                     CKGKVLDGVIDYGDYQTHALSQQEKDDGKALFCCATPQTDLVIECREISVGGAIRARI
                     MPCRVQKLEHVAHDVIVMWLKLPANERLQFLAGQYIEFLLKDGKRRAFSLANAPHNDD
                     LLELHLRLIPGGQFTEYVFNEMQEKTILRLEGPLGSFYLREDSDKPVIFIAGGTGFAP
                     IKGIIEHALHLKLQRDMRLYRGSLALRDLYLPELAPHWQAERGIAYVPVLSDPRPEDD
                     WSGRTGLVHKAVLEDIADLSGYQVYCCGAPAMVEAAHKDFVAAGLPEDEFFSDAFTFA
                     PRK"
     gene            1414..2211
                     /locus_tag="PKW44_05905"
     CDS             1414..2211
                     /locus_tag="PKW44_05905"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HQR51152.1"
                     /translation="MYWRYLLVTLEVLGITLLNYWMAGTYYSFEVLYCLPIIQTAHLS
                     SLRARRSSDKYLADFFGILSAVVWSLGEAAIAWPDFPMLALGLNIFTRSVTLAVLGRV
                     MTKLWRDRAYSRKDALTELANRLALFDRLEIEQTRSMRSGHPYSLMFIDIDQFKRLND
                     NQGHHTGDAALKVLAHVLLENSRTVDTVCRFGGDEFVLLFPETDEEGCSVLSRRISSA
                     AALEFWKKGWPISVSIGYTTEIGKNRSADELLRAADQKMYLLKKARQ"
     gene            complement(2268..2651)
                     /locus_tag="PKW44_05910"
     CDS             complement(2268..2651)
                     /locus_tag="PKW44_05910"
                     /inference="COORDINATES: protein motif:HMM:NF016102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HQR51153.1"
                     /translation="MYLLLLLLILLAFPALELVLLFKLAALIGGWLALYLLVSAVLGF
                     LLIQDERVVVFGRLVQAVQQGRHPLLALFASARKMIAGLLLIFPGIVSDVLALLLLIV
                     PLPSFRQPMPREEDGVIEGEWRRED"
     gene            2667..2984
                     /gene="cutA"
                     /locus_tag="PKW44_05915"
     CDS             2667..2984
                     /gene="cutA"
                     /locus_tag="PKW44_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019896579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HQR51154.1"
                     /translation="MNVILVLTNLPDRESAGKIAAHLIEKRLAACVNILAPCTSIYHW
                     QGKTESTQEIPLLIKTTEERYTELEQAIRALHPYELPEIIHVPVTGGLPAYLNWVSEE
                     VSS"
     gene            2984..4810
                     /gene="dsbD"
                     /locus_tag="PKW44_05920"
     CDS             2984..4810
                     /gene="dsbD"
                     /locus_tag="PKW44_05920"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047134 - protein-disulfide reductase
                     (NAD(P)) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HQR51155.1"
                     /translation="MLRILVLLFGLLAAPAFAADISSGKNALFDEPQGAILEPDQAFK
                     LAVGAVDGQTLKASFTIAPGHYLYRDRISFKLKPGSAGSVASVDLPPGEAKHDPNFGD
                     TAVYHHSFDATIHLQHGAAAPAAIALLASYQGCSATGLCYAPIRKTFDIALPVAGDTG
                     VAPAEPDENDRIESLLQGGRLWLVALGFFGFGLLLSLTPCVLPMIPILSSIIVGQGAH
                     PTRLHAFNLSLAYTLGMALAYTLAGIAAGLSGHLISSALQNPWVLGFGALLFVLLALS
                     MFGLYELQLPSGLESRVVGLTNRIKGGHFAGVFVMGMLSALILSPCVAAPLAGALLYI
                     GQTHDVLLGGVALFFLSLGMGVPLLAIGASAGALLPKTGPWMNAVRNFFGVVMLGMAV
                     WLIRPVIPAAVQLALWAALLIVPAIYLHALEALPPRASRWAKLWKGVGIIALVCGVAL
                     LLGALSGSKSPLQPLAGLYGATAEKPRALPFQRVESLSELEATIKQAKGKLIMLDFYA
                     DWCVSCKEYEQFTFSDPRVGRLLKDVILLQADVTANSPEDNAMLQRFGLFGPPGIIFF
                     DREGAEIPQVKTVGYQDADRFLVTLNTLYSRKDGECAPTLDC"
     gene            4786..5325
                     /locus_tag="PKW44_05925"
     CDS             4786..5325
                     /locus_tag="PKW44_05925"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HQR51156.1"
                     /translation="MRTDARLLIALLLAVMTTLAAVKALTPDRSKPANISSSEALFAA
                     SFPDASGKMQPLGQWRNRVLVVNFWATWCPPCREEMPELSGLQEKYQSGGLTVIGLAT
                     DDVDKMREYVRKNSLAYLLLAGDFEAASLAASLGNDKNVLPYTVVIGQDGRVVATYFG
                     RLDMRVLEQVLQPLLSISR"
     gene            5443..5889
                     /gene="aroQ"
                     /locus_tag="PKW44_05930"
     CDS             5443..5889
                     /gene="aroQ"
                     /locus_tag="PKW44_05930"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HQR51157.1"
                     /translation="MAEKSIIVLHGPNLNLLGLREPQHYGSATLDSINQRLTARAKSA
                     GATLEAFQSNSEAELVNKVQSLAGHEVDFILINPAAFTHTSVALRDALAAVKIPFIEV
                     HLSNVHARESFRHHSYFSDIAVGVICGLGAQGYELALEYAINNLQD"
     gene            5904..>6325
                     /gene="accB"
                     /locus_tag="PKW44_05935"
     CDS             5904..>6325
                     /gene="accB"
                     /locus_tag="PKW44_05935"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020163261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HQR51158.1"
                     /translation="MDLRKLKKLIDLVEESGISELELTEGEEKVRISRQPAGGQVQYA
                     PAYMQHPAMHTAPSAAPAAAPAAEPAAPVIDGHVVKSPMVGTFYRASSPEAKPFVDVG
                     TSVAVGDTLCIIEAMKLLNEIESDHGGVIKAILVENGQP"
BASE COUNT         1289 a         1938 c         1878 g         1220 t
ORIGIN      
        1 ggcaggaact gaaaagggcg agcgtgttca ggaaggcgct gacggcagaa acggctctgc
       61 tagccgcgct ggtactggtc gaagaaatag tgaacgaaat cgagaagctg aaaaaggcgc
      121 aggcagacgc ggagaaaacg aagcgctgaa tgcggcgcct gcccgatccc ccttgttgcc
      181 gccctacttt ctcggggcga aggtgaacgc gtcggagaag aactcgtctt ccggcaggcc
      241 ggcggcgaca aaatccttgt gcgctgcttc gaccatggcc ggggcgccgc agcagtagac
      301 ttggtagccg gagaggtcgg cgatgtcttc cagcacggcc ttgtggacca gtccggtgcg
      361 gccgctccag tcgtcctcgg ggcgcggatc ggacagcacc ggcacgtagg cgatgccgcg
      421 ctcggcttgc cagtgcggcg ccagctcggg caggtagagg tcgcgcaagg ccagcgagcc
      481 gcgataaagc cgcatgtcgc gctggagctt gaggtgcagc gcgtgctcga tgatgccctt
      541 gatgggagcg aagccagtgc caccggcgat gaagatgacg ggcttgtcgc tgtcctcgcg
      601 caggtagaag ctgccaaggg ggccttccag gcgcagaatg gttttttcct gcatttcgtt
      661 gaacacgtat tcggtgaact gcccgcccgg aatcaggcgc aggtgcagtt ccagcaggtc
      721 gtcgttgtgc ggggcgttgg ccagcgagaa ggcgcggcgc ttgccgtcct tgagcaggaa
      781 ttcgatgtac tggccggcga ggaactgcag gcgctcgttg gcgggcagct tgagccacat
      841 gacgatgacg tcgtgggcga catgttccag tttctgcacc cggcacggca tgattctggc
      901 gcgaatggcg ccaccgacgc tgatttcccg gcattcgatg accaggtctg tttgcggtgt
      961 ggcacagcag aacagggcct tgccgtcatc cttttcctgt tggctcagcg catgcgtctg
     1021 atagtcaccg tagtcaatca caccatccag caccttgccc ttgcacgcgc cacaggcacc
     1081 gttgcggcag ccgtagggca gattgaagcc ggcgtcgagc gcagcgctca ataccgtttc
     1141 atcgtcctga atggaaaact ggtggccgct gggctggatg gtgacttgga aggacatgtt
     1201 gggatattgg tactgaaaaa tggctggttc gcgcaaagcg cgcagtttac cataggcggc
     1261 cagaacaacg gccttgcttg cattgtggct gttccggcca aaataatcgc tggatttatc
     1321 catccatagg caagtagaat ccagtagacg gcaaaaaaac gatgtgaaaa cggcctcttg
     1381 catcccggcc cgagtcaaat taaggtaacg aacatgtact ggcgttattt actggttacg
     1441 ctcgaagtgc tgggcattac cctcctgaat tactggatgg cggggaccta ctattccttc
     1501 gaagtacttt actgcctgcc aatcatccag accgcacatt taagctccct gcgcgcgcgg
     1561 cgcagctccg ataaatatct ggctgatttt ttcggaatac tctccgctgt ggtgtggagc
     1621 ttgggcgagg ccgccattgc ctggccggat ttccccatgc tggcgctcgg cctgaacatt
     1681 tttacacgca gtgtgacgct ggccgttctc ggccgggtca tgaccaagct gtggcgcgac
     1741 cgcgcctatt cccgcaagga tgcgctgacg gagctggcca atcgcctggc gctgttcgac
     1801 aggctggaaa tcgagcagac gcgcagcatg cgctccggcc atccttactc cctgatgttc
     1861 atcgacatcg accagttcaa gcgcctgaac gacaatcagg gccaccatac cggggatgcg
     1921 gcgctgaagg ttctggcaca tgtcctactg gagaattcga gaacggtcga taccgtctgc
     1981 cgcttcggtg gcgacgagtt cgttctgctc tttcccgaaa cggatgaaga aggatgcagc
     2041 gtgctcagcc gtagaatcag ttcggccgcc gcgctcgaat tctggaaaaa aggctggccg
     2101 atctcggttt ccatcggata tacgacggaa atcggcaaga accgcagcgc ggacgaattg
     2161 ctgcgcgccg ccgatcaaaa aatgtacttg ctcaagaaag ccaggcaata gccgcggcct
     2221 gcaacacgag ggagcggccc tgcacaaccg gctgcgcgga gacttcccta atcctcccgc
     2281 cgccactcgc cctcgatcac gccgtcttct tccctgggca ttggctgcct gaaggatggc
     2341 agcgggacga tgagcagcag cagcgccagc acgtcgctga cgatgccggg aaaaatcagc
     2401 agcagcccgg caatcatttt tctggcactg gcaaacaggg ccagcagagg gtggcggcct
     2461 tgctgcacag cctgcaccag acgtccgaac accacgacgc gctcgtcctg aatcagcagg
     2521 aatccaagga cggcactgac cagcaaataa agcgccagcc acccgccgat caacgcagcc
     2581 agcttgaaca gcagcaccag ctccagcgcc gggaatgcga gtaaaatcag cagcagcaat
     2641 agcaaataca caatcaaacg atccttatga acgtcattct cgtcctgacc aacctgcccg
     2701 accgggaaag cgccgggaaa atagcggccc acctgataga aaagcgccta gccgcctgcg
     2761 tcaacatcct ggctccttgc acttcaatct accactggca gggcaaaacc gaaagcacgc
     2821 aggaaatccc gctgctgatc aaaaccaccg aagagcgtta caccgagctg gaacaggcca
     2881 tacgcgcgct tcatccctac gaacttcccg aaatcatcca tgtcccagtg actggcggcc
     2941 tgccagccta tttgaactgg gtatccgaag aggtttcgtc ctgatgctgc gtatcctagt
     3001 attgctgttc ggcctgctgg ccgcaccggc gtttgccgcc gatatttctt ccggcaaaaa
     3061 cgcgctcttc gacgaaccgc agggcgcgat tctggagccg gatcaggcgt tcaagctggc
     3121 agtcggcgct gtcgacggcc agaccctgaa ggccagcttc accatcgccc ccggccatta
     3181 cctctaccgc gaccgcatca gcttcaaatt gaagcccggc tcggcgggta gcgtcgcaag
     3241 cgtcgatcta ccgccgggcg aggccaagca tgaccccaat ttcggcgata ccgcggtcta
     3301 ccaccacagc ttcgatgcca cgatacacct gcaacatggt gcggctgccc cagccgcgat
     3361 cgccctgctg gcgagctacc aggggtgcag cgcaaccggg ctgtgctatg cgcccattcg
     3421 caagaccttc gacatcgccc tcccagtcgc cggcgatacc ggcgtggcgc ctgcggaacc
     3481 cgacgagaac gaccgcattg aatccctgct gcaaggcggc aggctgtggc tggtcgcgct
     3541 cggctttttc ggtttcggct tgctgctatc gctgacgccg tgcgtgctgc cgatgatccc
     3601 catcctgtcc agcatcattg tcggccaggg cgcgcacccc acccgtctgc acgccttcaa
     3661 cctgtcgctg gcgtacacgc tgggcatggc gctggcgtac acgctggcag gcatcgccgc
     3721 cgggctgtcc ggccatctca tcagttccgc cctgcagaat ccgtgggtgc ttggtttcgg
     3781 cgccttgctg ttcgtcctgc tggcgctttc catgttcggc ctctacgaac tgcaattgcc
     3841 gagcggcctg gaaagccgcg tggtcggcct gaccaaccgc atcaagggcg gacacttcgc
     3901 cggggtgttc gtcatgggca tgttgtcggc gctgatcctc agcccgtgcg tggcggcgcc
     3961 gctggcgggc gcattgctat acatcggcca gacccatgac gtgctgctgg gcggcgtggc
     4021 gctgttcttt ctgtccctgg gcatgggcgt accgctgctg gcaataggtg catcagccgg
     4081 cgccttgcta ccgaaaaccg ggccgtggat gaatgcagtg cgcaatttct tcggcgtggt
     4141 gatgctgggc atggcggtgt ggttgatcag gccggtgatt ccagcggcgg tgcaactggc
     4201 gctgtgggca gcgttgctga tcgtgccggc catctacctg catgcgttgg aggcgctacc
     4261 gcccagagcg agccgctggg ccaaattgtg gaagggtgtc ggcatcatcg cgctggtgtg
     4321 cggcgtggcg ctgctgctgg gggcgttgtc tggcagcaag agcccgttac agccgctggc
     4381 cggactatac ggagccactg ccgaaaaacc cagggcactg ccgttccagc gcgtggaatc
     4441 cttgagcgaa ctggaggcga cgatcaagca ggccaagggc aagttgatca tgctggactt
     4501 ctacgccgac tggtgcgtgt cctgcaagga gtacgagcag ttcaccttca gcgacccgcg
     4561 cgtcggcagg ctgctgaagg acgtgatcct gttgcaggcc gatgtcacgg caaattcgcc
     4621 ggaagataac gccatgctgc agcggttcgg cctgttcggc ccgcccggca tcattttctt
     4681 cgaccgggaa ggtgctgaaa tcccgcaggt caaaaccgtg ggctatcagg atgcggaccg
     4741 cttcctggtc acgctcaaca cgctttacag ccgcaaggac ggcgagtgcg caccgacgct
     4801 agactgctga tcgcccttct gctggcggtc atgacgactt tggcggcggt caaggcattg
     4861 actcccgacc gaagcaaacc cgccaatatc agcagcagcg aggcactgtt tgccgccagt
     4921 tttcccgatg ccagcggcaa aatgcaaccg ctcggccagt ggcgtaaccg ggtattggtg
     4981 gtcaacttct gggcgacctg gtgtccgccc tgccgcgaag aaatgccgga gctttccgga
     5041 ttgcaggaaa aataccagtc cggcggacta accgtgattg gcctcgctac cgatgatgtc
     5101 gacaaaatgc gggaatatgt gcggaaaaac tcgcttgcct acctgctgct cgcaggcgat
     5161 ttcgaagccg ccagcttggc tgccagcctc ggcaacgaca aaaacgtcct gccttatacc
     5221 gtggtgatcg gacaggatgg cagggtggtc gcaacttact tcggtcgcct cgatatgcgg
     5281 gttctggaac aagtcctcca gcccttgctc agcatctccc gctaaccata tgaaaaaact
     5341 ggaatttcgc taaatttcgg caattctctg gacaattacc acctatttgc ggcaaacttg
     5401 cacccttgca accacgcctg cgtgttgaaa aaacgactta aaatggccga aaaatccatc
     5461 atcgtgctgc atggccccaa cctcaatctg ctcgggttgc gggaaccgca acattacggc
     5521 agcgccacac tggacagtat caaccagcgc ctgacggcac gggcgaaatc cgccggcgcg
     5581 accctggaag ctttccagag caacagcgaa gccgaactgg tcaacaaggt gcaatcgctg
     5641 gccggtcacg aagtcgattt cattctcatc aatcctgctg ccttcaccca tacctcggtc
     5701 gctctgcgag atgcgctggc agcagtcaag ataccgttta tcgaagtaca cctctccaac
     5761 gtgcatgctc gcgagtcttt ccgccaccac tcctatttct ccgacatcgc tgtcggcgtc
     5821 atttgcggtc tgggtgcgca aggctatgag ctggcgctgg agtatgccat caataaccta
     5881 caagactgaa tctggggaca tccatggatc tgcgcaaact caagaaactg atcgacctgg
     5941 tcgaggaatc cggcatctcc gagctggaat tgaccgaagg cgaagaaaaa gtccgcatca
     6001 gccgccaacc ggccggcggc caagtgcagt acgctccggc ctacatgcag caccccgcga
     6061 tgcacacggc gccgtcggct gcacctgccg ctgcccccgc cgccgaaccg gctgcgccgg
     6121 tcattgatgg ccacgtggtc aagtcgccca tggtcggcac cttttaccgc gcctcgtcac
     6181 cggaagccaa gcccttcgtc gatgtcggca ccagcgtcgc cgtcggcgac acgctatgta
     6241 tcatcgaagc catgaagctg ctgaacgaga tcgaaagcga tcatggcggc gtcatcaagg
     6301 ccatcctggt cgagaacggg cagcc
//
LOCUS       DAOMFA010000095         6230 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_1606_length_6230_cov_4.542186, whole genome shotgun sequence.
ACCESSION   DAOMFA010000095 DAOMFA010000000
VERSION     DAOMFA010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 6230)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6230)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6230
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1606_length_6230_cov_4.542186"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..185
                     /locus_tag="PKW44_05940"
     CDS             <1..185
                     /locus_tag="PKW44_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HQR51159.1"
                     /translation="TPDQMLLIGDSLNHTQAARAAGCHVFCVPYGYNRGRHVNELDLD
                     AVVDSLLQAAKLIKKA"
     gene            297..1778
                     /gene="trpE"
                     /locus_tag="PKW44_05945"
     CDS             297..1778
                     /gene="trpE"
                     /locus_tag="PKW44_05945"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HQR51160.1"
                     /translation="MLHTITEQEFNDLARQGYNRIPLVAETYADLDTPLSLYLKLANQ
                     PYSYLLESVQGGERFGRYSFIGLPARTRIIAHGRNIRVERDGETLESVNDANPLDFVQ
                     AYQARFKPAPLGGLPRYTGGLAGYFGYDTVRYIERRLADKQKPDLLGTPDVLLLLTEE
                     LAVVDNLADKLTFIVYADPAQPSAYAAARDRLGTLIRSLRQPAEIPAAPALAPATAVS
                     EFGEAGFKAAVEQAKRYIFDGDIMQVVLSQRMSQAFPAPPLSLYRALRSLNPSPYMFY
                     YDMGDHHIVGSSPEILVRLEGDIVTARPIAGTRPRGKTREEDVALAEELLADPKELAE
                     HVQLMDLGRNDVGRVAQTGSVKVTDNMVIERYSHVMHIVSNVEGKLKPGMDAIDVLKA
                     TFPAGTVSGAPKVRAMEIIDELEPSKRGIYAGAVGYLGFNGDMDVAIAIRTAVIKAGT
                     LYVQAGAGIVADSVPESEWVETQNKARAVIRAAELALAGLDNS"
     gene            1785..4481
                     /locus_tag="PKW44_05950"
     CDS             1785..4481
                     /locus_tag="PKW44_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HQR51161.1"
                     /translation="MNQMLDWPESVNAEQVRQLYAGVPTSLITSSLLAILLAYVQSGA
                     VDGMNAFIWLILIELSFLARALLYRSWLLAPEKKDSSLWLRRFRIGTISSGVLWGLSA
                     FLLFPAHDVTYQATLAFALAGVTAGAMVSYCMDLVSVIGYTAPLMLPLIVQLFTESSD
                     AGLLMSGMTLLFLFFIVASAKRQHRHLIENVALRLQADAGKAQLLQKDADLKLLTERL
                     TAVIEAIPDAIFFKDGKGRWLITNKPAKRLYKLHGFRWRGKTGTELAKVRPELRTAHE
                     ASLVDDEYAWQAGKLTICQQLLVGTDATPHHYEIRKMPLFGSDGRRKALVVIARDVTE
                     LHAAERELRIAAAAFDVQEGIMITDAGNRILRVNRAFVTLTGYSAEEAVGNTPSLLRS
                     GRQSEEFYQSMWETLNRSRYWQGELWNRRKNGELFPVWLTITALIDTDGKVRNYIAAF
                     SDITQYKKAEEEIHNLAFYDYLTGLPNRRLLLDRVQQALVTSARHDQHGAILFIDLDN
                     FKTLNDTRGHAVGDLLLIEVAKRLSECVRAEDTVSRLGGDEFVVMLEGLSEVAWQAGG
                     QAELVGEKILDVLNQPYLLGEYEHHSTPSIGISLFRGSTESVEEILKHADTAMYQAKQ
                     SGRNTMRFFDPAMQKALESHILIEADLRQALPQQQLILYYQTQVDDARRVIGAELLIR
                     WNHPVHGMISPLEFIPVAEESRLIISIGTWVLQTACAQLKQWEQHPQASQLQLAVNVS
                     ARQFRQADFVAQVSAALQQHSANPALLKLELTESLVLDNVSDTVEKMQALKALGVGFA
                     MDDFGTGHSSLAQLKRLPLDQVKIDQSFVRDIATDPNDATIVQTIIAMTGALGLDVIA
                     EGVETEAQLQFLRENGCRKFQGYLFGQPVPVAEFEQLLITLH"
     gene            4544..5059
                     /gene="fabA"
                     /locus_tag="PKW44_05955"
     CDS             4544..5059
                     /gene="fabA"
                     /locus_tag="PKW44_05955"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019171 -
                     3-hydroxyacyl-[acyl-carrier-protein] dehydratase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-[acyl-carrier-protein] dehydratase
                     FabA"
                     /protein_id="HQR51162.1"
                     /translation="MQKQNSFTFEELLACGRGEMFGPGNAQLPLPPMLMFDRIISVTE
                     DGGEYGNGQVIAELDLRPDLWFFGCHFEGDPVMPGCLGLDAMWQLVGFFLGWMGGPGR
                     GRALGAGEVKFSGQVLPTAKKLTYRINMKRVIMRKLVMGIADATVEVDGREIYAAKDL
                     RVGLFTRTDNF"
     gene            5072..>6230
                     /gene="fabB"
                     /locus_tag="PKW44_05960"
     CDS             5072..>6230
                     /gene="fabB"
                     /locus_tag="PKW44_05960"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HQR51163.1"
                     /translation="MRRVVVTGLGIVSSLGNNKSEVLDSLREGRSGITFQQEYADRGL
                     RSHVAGSVKLNVQELIDRKLMRFMASGHAYAWIAMQEAIADAGLPPELVSNIRTGLIV
                     GAGGASHESILEGIDTLREKGVRRVGPYMVTKAMASGVSACLATGAQIKGVNYSISSA
                     CATSAHCIGNGVEQIQLGKQDIVFAGGAEEEHWTLSFMFDAMGALSSKYNDTPDKASR
                     TYDANRDGFVISGGGGILVLEEYEHAKARGAKIYAEIVGYGATSDGYDMVAPSGEGAV
                     RCMQMALQGVEGPVDYINTHGTSTPVGDVKELEAIRAVFGENGPAIASTKSLSGHALG
                     AAGVNEAVYTLLMMENNFITASANIETLDPAAEGLNIVRQRIDNANIQTAVS"
BASE COUNT         1277 a         1919 c         1877 g         1157 t
ORIGIN      
        1 tcacgccgga ccagatgctg ctgatcggcg attccctcaa ccatacccag gcagcgcggg
       61 cggccggctg tcatgtgttt tgcgtgccat atggttataa tcgcggtcgt cacgtgaacg
      121 aactggattt ggacgcggtg gtggacagcc tgctgcaggc agccaagttg atcaaaaaag
      181 catgaacccg gaattcttca atctgttgcg agccaactgg cgatggtggc gctgatctag
      241 ccgccctccg ccggcggtgc atgatggcca tcgcaaccta aatattggag atattcatgc
      301 tgcacaccat taccgaacaa gaattcaacg acctcgcccg ccagggctac aaccgcatcc
      361 cgctggtggc ggagacgtac gccgacctcg acacgccgct gtcgctctat ctcaagctgg
      421 ccaaccagcc ctattcctac ctgctggaat cggtacaggg tggcgagcgt ttcgggcgct
      481 actcgtttat cggcctgccg gcgcgcaccc gcatcatcgc ccatggccgt aacatccgcg
      541 tggagcgcga cggcgagaca ctggaatccg tcaatgacgc caacccgctg gattttgtcc
      601 aagcctacca ggcgcgcttc aagccggcgc cgctgggtgg gctgccacgc tacaccggcg
      661 gcttggccgg gtacttcggc tacgacacgg tacgctacat cgagcgccgc ctggccgaca
      721 agcaaaagcc ggacctgctg ggcacgccag acgtgctgct gttgctgacc gaagaactgg
      781 cggtggtgga caacctggcc gacaagctca ccttcatcgt ctatgccgac ccggcacagc
      841 ccagtgccta tgcggccgcg cgcgatcgtc tgggcacgct gatccgcagc ctgcgccagc
      901 cggctgagat tcccgccgcg cccgccctgg cgccggccac ggcggtttcc gaattcggcg
      961 aagccggttt caaggcggcg gtagaacagg ccaagcgcta cattttcgat ggcgacatca
     1021 tgcaggtggt actcagccag cgcatgtcgc aagccttccc cgcaccgccg ctgtcgctgt
     1081 accgcgccct gcgcagcctc aacccttcgc cctacatgtt ctactacgac atgggcgatc
     1141 accacatcgt cggctcctcg ccggaaattc tggtgcggct ggagggcgac atcgttaccg
     1201 cccgccccat tgccggcaca cgtccgcgcg gcaagacgcg cgaggaagat gtcgcgctgg
     1261 ccgaggaact gctggccgat ccgaaggagc ttgccgagca cgtacaattg atggatcttg
     1321 ggcgcaacga tgtcggccgc gtggcgcaga ccggctcggt caaggtcacc gacaacatgg
     1381 tgatcgagcg ctactcgcac gtcatgcaca tcgtctccaa cgtcgagggc aaactcaagc
     1441 ccggcatgga cgccatcgac gtgctcaagg ccaccttccc ggccggcacc gtatcaggcg
     1501 cacccaaggt gcgggccatg gaaatcatcg acgaactgga accaagcaag cgcggcatct
     1561 atgccggcgc ggtagggtat ctcggcttca acggcgacat ggacgtggcc atcgccatcc
     1621 gcaccgcggt catcaaggcc ggcacgctgt acgtgcaagc cggcgccggt atcgtggccg
     1681 attcagtgcc cgagagcgaa tgggtggaaa cgcaaaacaa ggcgcgtgcc gtgatccgtg
     1741 cggctgagct ggcgctggcc gggctggata atagctagac gaccatgaac cagatgctcg
     1801 actggcccga atccgtcaat gccgagcagg ttcggcaact ctatgccggc gtgccgacat
     1861 cgctgattac cagctccttg ctggcgatac tgctggccta tgtccaatcc ggcgctgtcg
     1921 atggcatgaa tgccttcatc tggttgatat tgatcgagct ttcatttctg gcccgcgccc
     1981 tgctttaccg ttcgtggctt ctggcaccag aaaagaaaga ttcctccctc tggctgagac
     2041 gtttccgcat cggcacgatt tcctccggtg tgctgtgggg gctgtcggcc tttttattgt
     2101 ttcccgcaca tgatgtgact taccaggcca cactcgcatt tgcgctggcc ggggtgactg
     2161 caggcgccat ggtgagttat tgcatggacc tggtcagtgt cataggctat accgccccac
     2221 tgatgctgcc gctgatcgtg caactgttta ccgagagcag cgatgccggc ctgctcatgt
     2281 cgggcatgac cttgctgttc cttttcttta ttgtcgcctc tgcaaagcgt cagcatcgtc
     2341 atctgattga aaacgtagcg ttgcgtctgc aggctgatgc aggcaaggca caactgttac
     2401 aaaaggacgc ggacctcaag ctgctcaccg agcgccttac cgcggtgatc gaggcaattc
     2461 cggatgccat tttcttcaag gatggtaaag gacgctggct gatcaccaac aagccagcca
     2521 aacgcctgta caagctgcac ggcttcagat ggcgtggcaa gacgggcacg gagcttgcca
     2581 aggtgcgccc cgagctccgt accgcacacg aggcaagtct ggtcgacgac gagtacgcct
     2641 ggcaggcggg caagctgacc atctgccaac agctgctggt gggcaccgat gccacgccgc
     2701 atcactacga aatacgcaaa atgccgctgt tcggcagcga tggccggcgc aaggcgctgg
     2761 tcgtaatcgc gcgcgatgtc acggagctcc atgcggcaga acgcgagttg cgcatcgcgg
     2821 cggccgcgtt cgatgtgcag gaaggcatca tgattacgga cgccggaaac cgcatcctgc
     2881 gcgtcaatcg cgcttttgtc acactgaccg gctacagcgc cgaagaggcc gtggggaata
     2941 ctccgagcct gctcaggtcc ggtcgtcaga gcgaggagtt ttaccagagc atgtgggaga
     3001 cgctcaatcg cagccgatac tggcaaggcg agctctggaa ccgtcgcaag aacggtgagc
     3061 tatttccggt atggctgacc atcacggcac tgatcgacac cgacggcaag gtgcgcaatt
     3121 atattgcggc cttttccgac atcacccaat acaaaaaagc ggaagaggaa atccacaacc
     3181 tggcctttta cgattatctc accgggctac ccaaccgccg ccttctgctt gaccgggtgc
     3241 agcaggcgct ggtaacttcg gcgcgccacg atcagcacgg cgccatcctg ttcatcgacc
     3301 tggacaactt caagacgctc aacgataccc gcggccacgc ggtgggggac ttgctgctga
     3361 tcgaggtggc gaaacgcctg agtgaatgcg tgcgtgcgga ggacacggtt tcccggttgg
     3421 gcggcgacga attcgtggtc atgctggagg gactgagcga ggtggcgtgg caggcaggcg
     3481 ggcaggcgga gctggtgggt gaaaaaatcc tcgatgtatt gaaccagcct tacttgctgg
     3541 gggaatatga gcaccacagc acacccagca tcggcatcag cctgttccgg gggagtaccg
     3601 aatcggtgga agaaatactc aagcatgccg ataccgccat gtaccaggcc aagcaatccg
     3661 gccgcaacac catgcggttt ttcgatccag ccatgcagaa ggcgctcgaa tcgcacatcc
     3721 tgatcgaggc ggatttgcgc caggcgctgc cacaacagca gctgattctc tactatcaga
     3781 cacaagtgga tgacgcgcgc cgtgtgatcg gtgcagaatt gctgatacgc tggaaccacc
     3841 cggtgcatgg catgatttca ccgctggaat tcatcccggt ggccgaagag tccagactga
     3901 ttatttccat cggtacatgg gtgctgcaaa ccgcctgcgc gcaactcaaa cagtgggaac
     3961 agcacccgca ggccagccaa ttgcagcttg ccgtgaacgt cagcgcgcgc cagttccgcc
     4021 aggcggattt cgtcgcccag gtcagcgctg ccctgcagca acattccgcc aacccggcgc
     4081 tgctcaagct ggagctgacg gaaagcctgg tgctggacaa tgtcagcgat accgtcgaaa
     4141 aaatgcaggc gctgaaagcg ctcggcgtgg gctttgccat ggatgatttc ggtaccggcc
     4201 actcttcgct ggcgcaactg aaacgactgc cgctggatca ggtcaagatc gaccagagct
     4261 tcgtacgcga catcgccacc gaccccaacg acgccaccat cgtgcagacc atcatcgcca
     4321 tgaccggcgc actgggcttg gatgtgattg cggaaggtgt agaaaccgaa gcccagttgc
     4381 aattcctcag ggagaatggt tgccggaaat tccaggggta cctgtttgga cagcccgtgc
     4441 ccgtcgccga attcgagcaa ttgctcatca cgctgcatta gggctttcgc ttggcgccgt
     4501 ttccggcgaa aatagcagcg aaattcaaac catggcgacc acaatgcaaa aacaaaacag
     4561 ctttaccttc gaggagctcc tcgcctgcgg gcgcggcgag atgtttggcc ccggcaacgc
     4621 acagctgccg ctgccgccga tgctgatgtt cgaccgcatc atttccgtca ccgaggacgg
     4681 cggcgagtat ggcaatggcc aggtcatcgc cgaactggac ctacgaccgg acctgtggtt
     4741 tttcggctgc cattttgaag gcgacccggt catgccgggc tgcctgggcc tggatgccat
     4801 gtggcagctg gtcggcttct tcctcggctg gatgggcggc cccggccgcg gccgggcact
     4861 gggcgcaggc gaggtcaaat tcagtggcca ggtgttgccg accgccaaaa agttgaccta
     4921 ccgcatcaac atgaagcgcg tcatcatgcg caagctggta atgggcatcg ccgatgccac
     4981 ggtggaggtc gatggccgcg aaatctatgc cgccaaggat ctacgtgtcg gcctgtttac
     5041 cagaacggat aatttttagg agggcgtgaa tatgcgtcgc gtcgtcgtca ccgggctggg
     5101 cattgtatcc agtctcggta acaacaaatc cgaggtgctc gacagcttgc gcgaggggcg
     5161 ctccggcatc acctttcagc aggagtacgc ggatcgcggc ttgcgctccc acgtggccgg
     5221 ctcggtcaag ctcaatgtgc aggaactgat cgaccgcaag ctgatgcgct tcatggccag
     5281 cggccatgcc tatgcctgga tcgccatgca ggaagccatc gccgatgccg gtctgccgcc
     5341 ggaactggtt tccaatatcc gcaccggcct catcgtcggt gccggcggcg cctcccacga
     5401 aagcatcctg gaaggcatcg acacattacg cgaaaaaggc gtgcgccgcg ttggcccgta
     5461 catggtgacc aaagccatgg ccagcggcgt ttctgcctgt ctggccaccg gcgcacagat
     5521 caagggtgtg aattattcca tcagctccgc ctgcgccacc agtgcccact gtatcggcaa
     5581 cggcgtggaa cagatccagt tgggcaagca ggacatcgtg ttcgccggcg gcgcagagga
     5641 agaacactgg acgctgtcgt tcatgttcga cgccatgggc gcactgtcca gcaagtacaa
     5701 cgatacgccg gataaggctt cgcgcaccta cgatgccaat cgtgatggct tcgtcatctc
     5761 cggcggcggc ggcattctgg tgctggaaga atacgagcac gccaaggcgc gcggcgccaa
     5821 gatctatgcc gaaatcgtcg gctatggcgc cacctcggat ggctacgaca tggtggcacc
     5881 gtccggcgaa ggcgcggtgc gctgcatgca gatggcgctg caaggcgtgg aagggccggt
     5941 ggactacatc aatacccacg gcaccagcac cccggtcggc gatgtcaagg aactggaagc
     6001 gatccgcgcc gtgttcggcg aaaacggccc ggccatcgcc tccaccaaat cgctgtccgg
     6061 ccacgcgctg ggcgcggcgg gggtcaacga ggcggtatac accctgctga tgatggaaaa
     6121 caacttcatc accgcttcgg ccaatatcga aaccctggac ccggcggcgg aaggcctcaa
     6181 tatcgtgcgt cagcgcatcg acaacgccaa catccagacc gccgtgtcga
//
LOCUS       DAOMFA010000096         6210 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_1613_length_6210_cov_4.797888, whole genome shotgun sequence.
ACCESSION   DAOMFA010000096 DAOMFA010000000
VERSION     DAOMFA010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 6210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6210
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1613_length_6210_cov_4.797888"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            307..1479
                     /locus_tag="PKW44_05965"
     CDS             307..1479
                     /locus_tag="PKW44_05965"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HQR51164.1"
                     /translation="MRCLLIFLMASLLAACAKEEAKPQTRPPTEVSVIKVVPQDTPVS
                     SEFVGQSESSRQVQIVARVNGFLDKRVYTEGSLVKAGQVMFLQDPKPLQATLDANKAA
                     LAEQRARLQTANADLDRVKPLATLNALSQKDLDDATGRQEAAAAAVQMAEADVEQAKL
                     NLGYTTITTPVTGLSSYARVQEGAYLNPANSLLTYVAQVDPIWLNFTLSENQLLERRT
                     EIDSGKLRVPPNKDFVVEAVLADGSVFPQKGHISFADAEFNKETGTYLLRATLPNPDA
                     TLRPGQFVRVRVSGDVRPNAILVPQQAVLQGAKGHFVFFIGKDGKAEIRGVKVGEWYG
                     KDWFISEGLETGDIVVTEGVMKLAPGAAVKIMNTAAAQAAATELKPAAASDTQPQK"
     gene            1495..4680
                     /locus_tag="PKW44_05970"
     CDS             1495..4680
                     /locus_tag="PKW44_05970"
                     /inference="COORDINATES: protein motif:HMM:TIGR00915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HQR51165.1"
                     /translation="MFTNFFIDRPIFSAVISIVISIAGLVAMVNLPIAQYPQITPVQI
                     QVTATYPGANAELVAQNVAAPIENQVNGANNMIYMSSTNSSTGNYTLTVYFTIDTDPA
                     LAQVDVQNRVSMAMAQLPQAVQVQGINVQQKTQSFLMIVALYSPDGRYDSDYIANYAN
                     VNVLDSIKRINGANQASIFGIPDYAMRIWLKPDRMAQLHVSTSDVANAVKQQNQQFAV
                     GRIGQSPTPYPVTQTFPATTNALTEPRQFEDIILRAENNDASIVRIRDVGYADLGRKD
                     YSIRSKYQGKPATLIVVYQQPGANAIEVSKNIKKMMVEQSKNFPKGLEYDIALDTTLF
                     VQASIQEVIHTFFEAVVLVVLVVFIFLHSLRLTIVPTLAVPVSILGALIGMLLFGFSI
                     NMLTLFGMILAIGIVVDDAIVVVENTERNMVEFGLSANAAAKKAMGEVAGPVIAIVLV
                     LNAVFIPVAFLGGITGALYKQFAVTIAISVVFSGIVALTLSPALAALLIQAKHGEKKG
                     FFKWFDQTFDRVTNGYVSLVKLSMRHWAISLIAFAFVIVGAVGLFKTLPTAFVPQEDQ
                     GYIFVPYFLPDAASLDRVDAVGAKAADFLNKNPAIANVTEVSGYSLLDSQNKTNYGLI
                     FTSFKDYEERKLPSMKADAIIADARKAYANIQEGLVMPVNPPAIPGLGITAGFQIWVQ
                     QKGGGNYAQLAEVVKQIIEKAKTDPRLAGVNTTISATGQQLLVEVDREKSEVLGVPIQ
                     DVYNTMQTMFGSLYVNQFPKDSRLWQVILQAEPKYRMTPEDMTRFYVKDREGKMVPLS
                     ALVNTRYVAGPDLATRFNNYPAIAINGAPAPGVSSGTALEAIREIAEQVMPESYGYEW
                     AGEAREEVSSGSTSALAFVFGLIFVFLILAAQYESWSLPVGVMMAVPFALLGALVAIA
                     MRGIANDIYFQIGLLTLIGLAAKNAILIVEFAVELRHKEGLSYFDAAAEAARLRLRPI
                     VMTSFAFILGVVPLAIASGASANSRHSIGTGVIGGTLAATMIAIVFIPMFYYVLSTMS
                     EKVFGSNKGAESHNTSQVQDDGNSTTKQEGH"
     gene            4682..6127
                     /locus_tag="PKW44_05975"
     CDS             4682..6127
                     /locus_tag="PKW44_05975"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HQR51166.1"
                     /translation="MRLRLLCVVVAAALGGCMVGPNYEKPVIETPNAYLYEPKEVAAE
                     ANIAWWQQYGDPVLDQLITDALAENKSVKIAAARVEQAAGVLTTQRAPLYPQVGYQGG
                     AGRERLSEESGAVSGLKNPDNYYQALVGVSWELDLWGRIRRLTEAAQANLYATQEARR
                     GVVLSLVSSVAFNYIQLRSLDEQLDISQRTLATYAESLRLMELQFQYGRVSLMNVEQA
                     KTRYQTAAAAIPQIRQQIVERENAISILLGRNPGPVPRGKSLSALALPEVPPGLPSEL
                     LEQRPDLLAAEQQLIAANAQIGAAKALYYPSISLTAALGGISTELGQLFSGAARVWNY
                     GGAVTGPIFTAGSIEGQVAQAEASHKALLLAYQQAIQNAFADVENALNSRTELSKQVD
                     AQEKLVKAQQNYARLAQLLYDGGYAPYSTVLQAEEQLFPSELNLATIRGQQLASLVNI
                     YKAMGGGWVDNATGMTEPAQVSESETQQNQN"
BASE COUNT         1366 a         1896 c         1724 g         1224 t
ORIGIN      
        1 tgaccaccat cccttacaag cagattgtca tcgccctggg ggacggcgca aaggcagcac
       61 tcggggcatt cgaccacctt atccgccatt gaagcaacgg cgtgagcacc gcaacaacgg
      121 gggcaatgcc cccgttgttt tttccagctt ttgaaaactc agttcaagtg cataattttc
      181 acaagcccca gtacccatcc tgatatacat tctgttatat ttcgcatggg tagagctcgg
      241 ccatccaggg aggatgacgg gtgcctcaat gcaatacgtt caacggtaat aacaaggatg
      301 tccgacatgc gctgcttatt gattttcctg atggcctccc tgctggcggc ctgcgccaag
      361 gaagaggcca agccccagac gcggccaccc accgaagttt ccgtcatcaa ggtcgtgccg
      421 caggatacgc cagtcagttc cgaatttgtc ggccaatctg aaagttcgcg tcaggtgcag
      481 atcgtggcgc gcgtcaacgg cttcctggat aagcgcgtct ataccgaagg cagtctggtc
      541 aaggcaggac aagtcatgtt cctgcaggat ccgaaaccgc tccaggccac actggatgcc
      601 aacaaagctg cgctggccga acagcgggca cgcctgcaaa cagccaacgc cgatctggat
      661 cgggtcaagc cactggccac gttgaatgcg ttgtcgcaaa aggacctcga cgatgccacc
      721 ggccggcagg aagcggcggc ggcggcggta cagatggccg aggcggatgt ggagcaggcc
      781 aagctcaacc tcggctatac caccatcacc acgccagtga ccggtctttc cagctatgcg
      841 cgcgtacagg aaggcgccta cctcaacccg gcaaacagcc tgctcaccta tgtcgcacag
      901 gttgacccga tatggctgaa tttcaccctg tcggaaaacc agttgctcga gcgcagaacc
      961 gaaattgaca gcggcaaatt gcgtgttccg ccgaacaagg attttgtggt ggaagccgtg
     1021 ctcgccgacg gcagcgtgtt cccgcaaaaa gggcacatca gcttcgccga tgcggagttc
     1081 aacaaggaaa ccggcactta tctcttacgg gccacgctcc ccaatcctga tgccaccttg
     1141 cgtcccgggc agttcgtgcg cgtgcgcgtt tccggcgatg tccgtcccaa cgccatcctg
     1201 gtgccgcagc aagcggtact gcaaggagcg aaggggcatt tcgtgttttt catcggcaag
     1261 gacggcaagg cagagatccg cggcgtcaaa gtcggcgagt ggtatggcaa ggactggttc
     1321 atatcggagg gactggaaac cggtgacatc gtagtgaccg aaggtgtgat gaaactggcg
     1381 cccggcgcag cggtaaagat catgaacacc gccgccgccc aagctgctgc gactgaactc
     1441 aaacctgccg ccgccagcga cacgcagccg caaaaatagc caggggctgc aacgatgttt
     1501 accaattttt ttatcgaccg tccgattttc tcagcggtga tttccatcgt catctcgatt
     1561 gccgggctgg tggccatggt caacctgccc attgcccagt atccgcagat taccccggtg
     1621 caaatccagg tgaccgccac ctaccccggc gccaatgccg aactggttgc acagaatgta
     1681 gcagcaccca tcgagaacca ggtcaacggc gccaacaaca tgatctatat gtcctcgacc
     1741 aactcgtcga cgggcaacta cacactgacg gtctacttca ccatcgacac cgaccctgct
     1801 ctggcgcagg tggacgtgca aaaccgggtc agcatggcca tggcacaact gccccaggca
     1861 gtacaggtgc aagggatcaa cgtccagcag aaaacacaaa gcttcctcat gatcgtggcg
     1921 ctgtactcgc ctgacggacg ctatgactcg gattacattg ccaattacgc caatgtgaat
     1981 gtgctggatt cgatcaagcg catcaacggt gccaaccagg cctcgatttt cggtatcccg
     2041 gactacgcca tgcgcatctg gctcaagccg gaccgcatgg cacaactgca cgtttctacc
     2101 agtgatgttg ctaatgcagt caagcagcag aaccagcagt tcgcggtcgg ccgcatcggc
     2161 caatcgccaa cgccctatcc ggtaacccag acctttccgg caaccacgaa tgcgcttacc
     2221 gagccgaggc agttcgagga catcattctc cgcgccgaga acaacgatgc ctctatcgtg
     2281 cgcatcaggg atgtgggcta tgccgacctc ggacgcaagg actactcgat acgctcaaag
     2341 taccagggta aaccggctac cctaatcgtc gtttaccagc aaccgggcgc caatgccatc
     2401 gaggtttcaa aaaacataaa aaaaatgatg gtggagcagt cgaagaattt tcccaagggg
     2461 ttggaatacg acatcgcact tgacacgacc ttgttcgtcc aggcttccat ccaggaagtc
     2521 atccacacct tcttcgaggc cgtggtgctg gtggtgctgg tggtattcat cttcctgcac
     2581 agcctgcggc tgaccatcgt ccccaccctg gcagtgccgg tttccattct gggcgccctg
     2641 atcggcatgc tgctgttcgg cttttccatc aacatgctga ccttgttcgg catgatcctc
     2701 gctatcggca tcgtggtaga cgatgccatc gtggtggtcg agaacaccga gcgcaacatg
     2761 gtggagttcg ggctgagtgc aaacgccgcc gcaaagaaag cgatggggga agtggccggt
     2821 ccggtcattg ccatcgtgct ggtactcaat gccgtgttca tcccggtcgc tttcctcggc
     2881 ggcattaccg gagcacttta taaacaattc gccgtcacca ttgccatttc ggtggtgttc
     2941 tctggcatcg tcgcgctgac cctgtctccc gccctggcgg cactgctgat ccaagccaag
     3001 catggcgaga aaaagggctt cttcaaatgg tttgaccaaa cctttgaccg cgtgaccaac
     3061 ggctatgtca gcctggtgaa gctgtccatg cgccactggg cgatcagcct gatcgccttt
     3121 gccttcgtca tcgtcggcgc tgttggtctg ttcaaaacgc tgccgacggc cttcgtaccc
     3181 caggaagacc agggctatat ctttgtccct tattttctgc ctgatgccgc cagtctcgac
     3241 cgtgtcgacg cagtcggcgc caaagcggca gatttcttga acaagaaccc tgccatcgcc
     3301 aacgtcaccg aggtcagcgg ctacagtctg ctggattcgc agaacaaaac caattatggc
     3361 ctgatcttta cctcattcaa ggattatgag gagcgcaagc tgccgtcgat gaaggctgat
     3421 gcgatcatcg ccgatgcgcg caaagcctac gccaacatcc aggaaggact ggtcatgccg
     3481 gtcaacccac cggcgatccc cggcctaggc attaccgccg gtttccagat ctgggttcag
     3541 caaaaaggcg gtggcaacta tgcgcaactg gccgaggtgg tgaaacagat tatcgagaaa
     3601 gccaagaccg acccccggct cgccggcgtc aatactacga tcagcgccac cggccaacag
     3661 ctgctggtcg aggtcgaccg tgagaaatcg gaagtattgg gcgttcccat ccaggacgtt
     3721 tacaacacga tgcagaccat gttcggctcc ctgtacgtca accagttccc caaggactcg
     3781 cgcctgtggc aggtgattct tcaggcggaa cccaagtacc gcatgacgcc cgaagacatg
     3841 acgcgctttt acgtcaagga ccgtgaaggg aaaatggttc ccctgtctgc gctggtcaat
     3901 acccgctacg tcgccgggcc cgatctggcg acccgcttca acaactatcc ggccattgcc
     3961 atcaatggtg cccccgcccc tggggttagt tccggtaccg cgctggaggc tatccgagaa
     4021 atcgctgagc aagtcatgcc ggaaagctac ggctacgaat gggcgggcga agcgcgcgag
     4081 gaagtgtctt cgggttcaac ctcggcactg gctttcgtct ttggtctgat cttcgtgttc
     4141 ctcatccttg cggcacagta cgaaagctgg tcgttgcctg tgggcgtcat gatggccgtg
     4201 ccgttcgcgc tgctgggggc gctggtcgca attgccatgc gcggcattgc caacgacatc
     4261 tatttccaga tcggcctgct gaccctgatc ggcctggcgg cgaagaacgc catcctgatc
     4321 gtggaatttg ccgtagaact gcgccacaag gagggtctgt cctactttga tgcggcggcc
     4381 gaggctgccc gcctgcggct gcgtcccatc gtcatgacct ccttcgcctt tattcttggc
     4441 gttgttccgc tggccatcgc cagcggcgcc tcggccaaca gccgccactc catcggcacc
     4501 ggggtcatcg gcggcacact ggcagccacg atgatcgcca tcgtctttat cccgatgttc
     4561 tattacgttc tgtccaccat gagcgagaag gttttcggca gcaacaaggg cgcggagtcg
     4621 cataacacat cgcaagtaca ggatgacgga aactcaacaa ccaagcagga ggggcactga
     4681 catgcgactt cgtctcctct gtgttgtcgt agccgctgcc ttgggcggtt gcatggtcgg
     4741 ccccaattac gaaaagccgg tgatcgaaac acccaatgcc tacctgtacg aacccaagga
     4801 agtcgcggca gaagccaaca tcgcgtggtg gcagcagtac ggcgacccgg tactggatca
     4861 actgatcacc gacgcactgg ctgaaaacaa gagcgtcaag atcgcagcgg cacgcgtgga
     4921 acaagcggct ggcgtactca cgacccaacg cgccccactc tatccacagg tcggctatca
     4981 gggtggcgcc ggacgcgagc ggctttcgga agaaagtggg gctgtgtcgg ggctcaagaa
     5041 cccggataat tactatcagg cactggttgg cgtcagctgg gagctcgact tgtgggggcg
     5101 catccgccgc ctgactgaag ccgcccaggc taatctgtac gccacgcaag aagcacgccg
     5161 cggagtcgtg ctcagcctgg tttcctcggt tgccttcaac tacatccagt tgcgcagtct
     5221 ggacgagcaa ctcgacatat cgcaacgtac actggccact tatgccgaat cgctgcgtct
     5281 gatggaactg caattccagt atggccgcgt ttccttgatg aacgtggagc aggccaagac
     5341 acgctatcaa actgcagcag cggctatccc tcagatcagg caacagatcg tggagcgcga
     5401 gaacgccatt tccatcctgc tgggacgcaa ccccggcccg gtcccgcgcg gcaagagcct
     5461 gtctgcacta gccctgccag aggtaccccc cgggctgcct tcggagctgc tggaacagcg
     5521 ccccgacctg ctggcggcag agcagcaact gatcgccgcc aatgcccaga tcggcgccgc
     5581 caaggcgctt tactacccga gcatctcact caccgcggca ctaggcggca tcagcacgga
     5641 actgggtcag ttattctccg gcgcggcgcg tgtctggaac tatggtggcg ctgtcaccgg
     5701 accgattttc accgccggct cgatcgaggg tcaagtagct caggccgagg cttcgcacaa
     5761 ggcccttctg ctcgcttacc agcaggccat ccagaacgct tttgccgacg tcgaaaatgc
     5821 gttgaattcg cgtaccgaat tgagcaagca ggtcgatgct caggagaagc tggtcaaggc
     5881 gcaacaaaac tatgcccggc tggcacagct tttatacgat ggcggctacg ctccctactc
     5941 caccgtcttg caggcagaag agcagctgtt cccttcggaa cttaacctgg ccacgatacg
     6001 cggccaacag ctggcttcgc tggtcaacat ctacaaggcc atgggcggcg gctgggtaga
     6061 caatgccacc ggcatgaccg aaccggcgca agtcagtgaa tcagaaacgc aacaaaatca
     6121 gaattagcag gcctatagag ttcacgaggg aaattgcatg agcgagcaca cccccgacgg
     6181 cgtcacccaa gccatgatta tgcattttca
//
LOCUS       DAOMFA010000097         6147 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_1638_length_6147_cov_6.048424, whole genome shotgun sequence.
ACCESSION   DAOMFA010000097 DAOMFA010000000
VERSION     DAOMFA010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 6147)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6147)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6147
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1638_length_6147_cov_6.048424"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(310..1473)
                     /locus_tag="PKW44_05980"
     CDS             complement(310..1473)
                     /locus_tag="PKW44_05980"
                     /inference="COORDINATES: protein motif:HMM:NF033085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase"
                     /protein_id="HQR51167.1"
                     /translation="MKNRLFTVLLTAAVFGLGSAVNAAEISSAEAMKAISAAVLPAME
                     KNQIPGMAVALVLGDKTYVLNYGVSDVEKQTPVTDETIFEIGSISKTFTATLATHAEA
                     TGKLKLTDTAAKYLPELSGTDFGNLQLFHLGTHTVGGIPLQVPDEVQTREQLLEYLRT
                     WKPAYKTGTMRTYANPSIGALGWITAKSLGQDFSTAMTQTIFQPLGLTSTYLSIPAQK
                     MSSYAWGYNNDKKPVRVNPGMLDSEAYGIKTTATDLITFVKANMGMLPLDSMLQSALN
                     NTRKSYFSVGQMTQDLIWEEYAMPVDLPMLQEGNAPKLILNPTPVSAKVPAATPNSNV
                     WVNKTGATNGFGAYVAFVPEKRFGVVLLANKNYPNAERVEIAHKIYSNLTGKQ"
     gene            complement(1691..1975)
                     /locus_tag="PKW44_05985"
     CDS             complement(1691..1975)
                     /locus_tag="PKW44_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011342942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin, RelE/StbE family"
                     /protein_id="HQR51168.1"
                     /translation="MRVVWTPEAQQDRADVWDYIAADNPRAAARMDEIFSDAAARLIQ
                     HPMLGKPGKIPGTRELIPHESYRLVYQIDGETVWILTLVHTARLWPPVRD"
     gene            complement(1972..2250)
                     /locus_tag="PKW44_05990"
     CDS             complement(1972..2250)
                     /locus_tag="PKW44_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011342941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin of toxin-antitoxin stability system"
                     /protein_id="HQR51169.1"
                     /translation="MSKQAVFTMKLDPELRAEFMAEAEAAHRPASQVLRELMREFIQS
                     QRKSREYDEFLRRKVEAGRASMRAGLGRSNDEVEAEFSARRASVASQS"
     gene            2489..3079
                     /locus_tag="PKW44_05995"
     CDS             2489..3079
                     /locus_tag="PKW44_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HQR51170.1"
                     /translation="MLVGYMRVSSDSDRQSTDLQRDALLAAGVDARHLFEDHASGAKD
                     DRAGLARALEFVRPGDVLVVWKLDRLGRSLSHLLALVTSLKDKRVAFRSLTENLDTTT
                     PSGEFLFQVFGALAQYERALIQERVVAGLAAARKRGRIGGRPQAITGEKLDAIIAALE
                     GGMSKAAVCRNFGVKRTTLIETLTRAGWPASVPPGA"
     gene            3084..6047
                     /locus_tag="PKW44_06000"
     CDS             3084..6047
                     /locus_tag="PKW44_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003118422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HQR51171.1"
                     /translation="MADGGGSTEDQWVLAPAERELVMTKNRANRLGFAILLTFFRDRG
                     RFPRDETEVEVQGIAALAKQLDAPAPLDGEAFLTGRTAERLRGEIRVRFGFREATVAD
                     AEMLTEWLRDHVAGEVGGDIEPMIERLEARCRELAIEPPTPERMERLARSALRAHEDR
                     FHKSVFERLPPPTRERLDALLRPEKTDSEESATDAQGEAAGSAPAVLLKLRGSPGRPG
                     LASMRDELEKLELIRGIELPADLFDQASSRDLERCRQRVSVEVPRDLRRHPDGARLTW
                     LAAFVYLRARSLTDDLVDLLIETIHQIGARAERKVERELLEDLKRVSGKQNLLFNLAD
                     ATLAQPDGVVRDVVFPVVGEQTLRDLVKEWKATGPTYRITLRTVIRNSYQGHYRRMVP
                     TLLAALEFRSNNDRHRPVMDALDLVKRFADTKVHTFPADMEVPLDGVVRGLWREAVME
                     RDAAGRDRVNRVTYEIAVLEALRERLRCKEIWVVGANRYRNPDDDLPADFEQNREDYY
                     RALNLPLDVERFIADLQAEMRAALSTFDAGLKKNPSVRLSSKGGGWITLTPLDAQPDP
                     PNLTALKAELNVLWPMTSLLDMVKEADLRLSFTDALKSPTSYESMDRSVLQPRLLLCL
                     HGLGTNAGLQRMAGLDSGTTARDLAYVRRRYISVDAMRRAIAIVADGTLQARNPAIWG
                     SGTTACASDSKHFGAWDQNLTTQWHVRYGGRGVMFYRHVERSSLCIHSQLKSPSSSEV
                     ASMIEGVIHHCTEMEVDRQYVDSHGQSTVAFAFCRLLGFQLLPRLKAIHSQKLYRPET
                     GKADAYANLQQILTKPIDWDSVRQQYDQMVKYATALRLGIGETEAILRRFTKKNVQHP
                     TYKAFAELGKAIKTIFLCRYLHDEALRREINEGLNVVEQWNGATDFVFFARRGEMASN
                     RREDHEVSMLALHLIQNCMVYINTLMIQKVLAQPHWQGRFTPRDYAALTPLIWEHVNP
                     YGRFDLDMNTRLDLP"
BASE COUNT         1212 a         1886 c         1849 g         1200 t
ORIGIN      
        1 cgacccgcaa caccgccacc ttagccgcaa gtccatcagc acccgaaacc cgttttgcta
       61 ggggtagatc aagcagtcgt gtcaccagcc gccatttcga tcacggcaat ggcaccctgg
      121 cgttcacgtc caaattgttc cggtttggat gaggccgcct gacgtctagg atggcgcgtg
      181 acatacgagc gctgtgactc ctgcagggga gactcaacag ccggaccgga cggcgagcat
      241 cggcccatct cacgtatttg tcatgtcagc tttgacactg cacacgcgac ggcagccgac
      301 ccaaagctgt cattgtttcc cggttagatt gctataaatt ttgtgggcaa tttctacccg
      361 ttcagcattg gggtagtttt tattggccag caacacaacc ccgaagcgct tttcaggcac
      421 aaacgcaaca taagcaccga agccgttggt agcaccagtc ttgttaaccc acacattgct
      481 gttaggtgtg gctgcaggaa ccttagcgct tactggggtt ggattcagga taagtttggg
      541 agcattgcct tcttgcagca taggcaagtc aacaggcatt gcatactcct cccagatgag
      601 gtcttgagtc atctggccta cgctgaaata gctcttgcgg gtattgttta aagccgattg
      661 cagcatgcta tccaagggca acatgcccat gttggccttc acaaaggtga tcaggtcagt
      721 agccgtggtt ttgatgccgt aggcttcact gtccaacatg cctggattga cacgcacagg
      781 cttcttatcg ttgttgtagc cccaagcgta gctggacatc ttctgtgcag ggatgctcag
      841 ataagtgcta gtcaaaccga ggggctggaa aatggtttga gtcatggcgg tgctgaaatc
      901 ttgccccaaa cttttcgccg ttatccaccc taaagcaccg atgcttgggt ttgcataagt
      961 acgcatcgtg ccggttttgt acgcaggctt ccaggtgcgg aggtattcca gaagctgctc
     1021 ccgagtttgg acttcatcag gcacctgcag aggaatgccg ccaacggtgt gcgttccaag
     1081 atggaaaagc tgcaagttac cgaagtccgt accagacaac tcaggcaaat actttgctgc
     1141 tgtatcggtc agtttcagct tcccagtggc ttctgcgtgc gtcgccagtg tggctgtgaa
     1201 ggtcttactg atagagccaa tctcaaaaat cgtctcgtca gttaccggtg tttgtttctc
     1261 aacatctgac acgccatagt ttaagacata agtcttgtcc cccaatacca atgccacagc
     1321 cataccagga atctggttct tttccatggc aggcagcacg gctgcagaga tggctttcat
     1381 tgcttcagca ctggaaattt cagcagcatt gacagccgag ccaagaccaa agacagcggc
     1441 tgtcagaagg acagtaaaca gacggttttt caatttttac cttacgggtg aaggaggaaa
     1501 gccaacgctg gcacttgcga tgcaattgag tgctgtagcg ctacgagctg gggattctaa
     1561 aaaatttgat agcaaattgg ctcggcatcg cggagcgatg agtgactgct tctggccgcc
     1621 tgccgccgac cgcggtctgg ctcgaaagca gtctgtcaac gcgccgtttc attgttgcca
     1681 tgatcatctc ttaatcgcgc acaggcggcc acaggcgggc cgtatgaacc agcgtcagta
     1741 tccacaccgt ttcgccgtcg atctggtaca ccaggcgata gctttcgtgc gggatcaact
     1801 cgcgggtccc gggaatcttt cccggcttgc ccagcatggg gtgctggatc aaccgggcgg
     1861 ccgcgtcgct gaaaatctca tccatccggg ccgccgcgcg cggattgtcg gctgcgatgt
     1921 agtcccacac atcggcacgg tcttgctgcg cttcgggcgt ccaaacaact ctcacgactg
     1981 gctcgccaca ctggcacgcc gtgcggagaa ttcggcctca acttcatcgt tcgatcgccc
     2041 caatccagcg cgcattgaag cccggccggc ctcgaccttg cggcgcagga actcgtcgta
     2101 ctcgcgcgac ttgcgctggc tctgaatgaa ctcgcgcatc aactcgcgca gcacttgcga
     2161 cgccgggcga tgggccgcct cggcttcggc catgaactcg gcgcgcaact cgggatccag
     2221 cttcatcgtg aaaacagctt gttttgacat gatcggggcc tcctgccact tgatactaac
     2281 aaagtatata cgccgtcatt actaagcgct attcatagaa cgctgcaagg cgggcgtgcg
     2341 ctatgccaag gcctgtcgga aaacatttgt ttgtcgacag atcttcaaca gccctctgca
     2401 ccaagctccg agtggccgca aaattgtgcg gaaaactctg tcgccagacg ctaccatgcg
     2461 gaaacctctt cttgatggtt ttccgcttat gctggtaggt tacatgcgtg tgtcgtcgga
     2521 ttccgaccgg cagagcacgg atttgcagcg cgatgcgctg ctcgccgcgg gcgtcgatgc
     2581 gcggcatctg ttcgaggatc atgcttccgg cgcgaaggat gaccgtgcgg gcctggcgcg
     2641 ggcgctcgaa ttcgttcgcc ccggcgacgt gttggtcgtg tggaagctcg accggctcgg
     2701 ccgttcactg tcgcatctgc tcgccctggt gacctcactc aaggacaagc gggtggcgtt
     2761 ccgctcactg acggagaacc tggacaccac cacgccatcg ggcgagtttc tgttccaggt
     2821 gttcggcgcg ctcgctcagt acgagcgcgc cttgatccag gagcgtgtcg ttgccggcct
     2881 ggctgctgcc cgcaaacgtg gccggatcgg cggccggccg caggcaatca ccggcgagaa
     2941 gctggatgcc attatcgccg cgctcgaagg tggcatgtcc aaggcggccg tgtgccgcaa
     3001 cttcggcgtg aagcggacca ccttgatcga aactctgacg cgggccggct ggcccgcgtc
     3061 agtgccccca ggagcgtagc ggtatggcgg acggcggcgg cagtacggaa gatcagtggg
     3121 tattggcgcc agccgaacgc gaactggtga tgaccaaaaa ccgggcgaac cggttgggct
     3181 ttgccatcct gctgaccttc ttccgcgatc gcggccgttt cccgcgcgac gaaaccgaag
     3241 tcgaggtaca gggcatagcc gcgctcgcca aacaacttga cgcacccgcg cccctcgacg
     3301 gcgaagcctt cctcacgggc cgcactgccg agcggctgcg cggcgaaatc cgtgtgcgtt
     3361 tcggcttccg cgaagcgacg gtggccgatg ccgagatgct gacggagtgg ctgcgtgatc
     3421 atgttgccgg agaagttggc ggtgacattg agccgatgat cgagcggctg gaagcgcgct
     3481 gccgcgaact cgccatcgag ccgccaacgc ccgagcggat ggagcggctt gcgcgcagcg
     3541 cgttgcgtgc ccacgaagac cgtttccaca aaagcgtctt tgaacgcttg ccgcccccga
     3601 cccgcgagcg tctggatgcc ttgctgcgcc cagagaagac cgacagcgag gaaagcgcta
     3661 ctgatgctca aggggaagcc gccggcagcg ccccggccgt tctgctgaag ctgcgcggca
     3721 gtcctggccg tcccggcctt gccagcatgc gggacgaact ggagaagctg gaactgatcc
     3781 gtggcatcga gctgcccgcc gatctcttcg atcaagcgtc atcgcgcgac ctggaacgct
     3841 gccggcagcg cgtatcggtc gaggttcccc gcgacctgcg ccgacatccc gatggagcgc
     3901 gcctcacctg gctggccgca ttcgtctacc tgcgcgcccg cagcctgacc gacgacctgg
     3961 tggacttgct gatcgagacc atccaccaga tcggcgcgcg tgccgaacgc aaggtcgaac
     4021 gcgaactgct ggaggacctc aagcgcgtgt ccggaaagca gaacctgctg ttcaatctgg
     4081 ccgacgccac cttggcccag ccggacggcg tggtgcgcga cgtggtgttt ccagtggtcg
     4141 gcgagcagac gctgcgcgat ctggtcaagg agtggaaggc caccggccca acctaccgca
     4201 tcaccctgcg cactgtgatc cgcaattcct accagggcca ctaccggcgc atggtaccga
     4261 ccttgctggc cgcgctggaa ttccgctcca acaacgaccg ccaccgcccg gtgatggacg
     4321 cgctcgacct ggtgaagcgc tttgccgaca ccaaggtgca taccttcccg gccgacatgg
     4381 aggtgccgct cgatggcgtg gtacgtggcc tgtggcgaga agccgtcatg gagagggacg
     4441 ccgccggccg ggatcgggtc aaccgcgtca cctatgaaat cgccgtgctg gaagccctgc
     4501 gcgagcggct gcgctgcaag gaaatctggg tggtcggcgc gaaccgctac cgcaaccccg
     4561 acgacgatct gccggctgac ttcgagcaaa accgcgagga ctactaccgg gcgctgaacc
     4621 tgcctctcga tgtggagcgc ttcatcgccg acttgcaggc cgaaatgcgc gcggcgctgt
     4681 ccaccttcga cgctggcttg aagaagaacc catccgtccg gctgagcagc aagggcggtg
     4741 gctggatcac gctgacgccg ctcgatgcgc aacccgatcc ccccaatctg accgcgctaa
     4801 aggccgaact caatgtcctc tggccgatga ccagcctgct cgatatggtc aaggaagccg
     4861 atctgcggtt gagctttacc gatgccctga aaagcccgac ctcctacgag tcgatggatc
     4921 gctcggtgtt gcagccgcgc ctgctcctgt gtctgcacgg cctgggcacc aatgctggct
     4981 tgcagcgcat ggccgggctg gattccggca ccacggcccg cgacctggcc tatgtgcgcc
     5041 gccgttacat cagcgtggac gcgatgcgcc gcgcgattgc catcgtcgca gacggcacgc
     5101 tgcaagcccg caacccggcg atctggggta gcggcaccac cgcttgcgcg tcggactcga
     5161 aacacttcgg cgcgtgggat cagaacctca ccacgcaatg gcacgtccgc tacggcgggc
     5221 gcggcgtgat gttctacagg cacgtcgagc gcagctcgct gtgcatccac tcgcagctca
     5281 agtcgccgtc gtcatcggag gtggcgtcga tgatcgaggg cgtgatccac cattgcaccg
     5341 agatggaggt ggatcggcag tatgtcgatt cgcacggcca gagcacggtg gcgttcgcct
     5401 tctgccgcct gctgggcttc cagttgctgc cacggctgaa ggccatccac tcacagaagc
     5461 tgtaccggcc agagaccggc aaggccgacg cctacgcgaa cctgcaacag attctgacca
     5521 agcccatcga ctgggactcg gtgcggcaac agtacgacca gatggtcaag tacgccaccg
     5581 cgctgcgcct ggggataggg gaaaccgaag ccatcctgcg ccgcttcacc aagaagaacg
     5641 tgcagcaccc gacctacaag gcattcgctg agctgggcaa ggcgatcaag actatcttcc
     5701 tgtgccgcta cctgcacgac gaggcgttgc gccgggaaat caacgaaggg ctgaacgtag
     5761 tcgagcagtg gaacggcgcg accgacttcg tgttcttcgc ccgccggggc gagatggcga
     5821 gcaaccgccg cgaggatcac gaggtcagca tgctcgcgct gcacctgatc cagaactgta
     5881 tggtctacat caacacgctg atgatccaga aggtgctggc acagccgcat tggcagggca
     5941 ggttcacacc acgcgactat gccgccctga cgccgctgat ctgggaacac gtcaacccgt
     6001 atggtcggtt cgatctcgat atgaacaccc ggctcgacct gccgtgatcg agatggcggc
     6061 tggtgacacg actgcttggt ctacccctgc tagggggatt cgatgcacgc taggggggga
     6121 ttcgatgcac gctagggggg gattcga
//
LOCUS       DAOMFA010000098         5997 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_1698_length_5997_cov_5.598788, whole genome shotgun sequence.
ACCESSION   DAOMFA010000098 DAOMFA010000000
VERSION     DAOMFA010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 5997)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5997)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5997
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1698_length_5997_cov_5.598788"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..536
                     /locus_tag="PKW44_06005"
     CDS             <1..536
                     /locus_tag="PKW44_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HQR51172.1"
                     /translation="HMVIIGLFLGGLIPYLFGAMAMEAVGRAASSVVVEVRRQVKEIP
                     GIMNGTEKPDYSRAVDMLTKSAIKEMIVPSLLPVLVPILVGKFLGAQALGGLLIGTIV
                     TGLFVAISMTVGGGAWDNAKKHIEDGHHGGKGSEAHKASVTGDTVGDPYKDTAGPAIT
                     SLIKIINIVALLIVSIL"
     gene            complement(625..1041)
                     /locus_tag="PKW44_06010"
     CDS             complement(625..1041)
                     /locus_tag="PKW44_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HQR51173.1"
                     /translation="MRRLDALFKDNAELQALANQTGRLAELQKTWSEVVPQPLQPFTR
                     AGGLQHRRITVFADNGAVAAKLKLLAPNLLKSLQIKGLEVTSIRVEVQVKSQARASAG
                     VARHLSSKASASLEKLAEKLPDSPLRTALERLAKRG"
     gene            1098..1988
                     /locus_tag="PKW44_06015"
     CDS             1098..1988
                     /locus_tag="PKW44_06015"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013149060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HQR51174.1"
                     /translation="MNIIFVSNNMAKARTLSLRQALALLAGLLIVPPLLTAALILPQP
                     GAEQQGMQALLPMRLIQAVRDNSQAHLDALAMQLGQIQARVMRLDALSERLAKLAGVD
                     DKEIESDSQTPGQGGPLLQARSLTADEIGRQMAELTRQLEERNDRLGVLEALLLQQNL
                     KHTTIPNSSPVEAAYNSSSFGWRNDPFTGQMAFHEGLDFTAEKGTPIYAAADGIVSQA
                     ESTPDYGNIVKVDHGSGLETRYAHASRILVKPGERVEKGQLIAEVGNTGRSTGAHLHF
                     EVRLNGVALDPRKYLQNRNS"
     gene            2047..>3933
                     /gene="secA"
                     /locus_tag="PKW44_06020"
     CDS             2047..>3933
                     /gene="secA"
                     /locus_tag="PKW44_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HQR51175.1"
                     /translation="MLSTVFKKIFGSRNERLIKQYAQVVRTINALEEATQALSDDQLR
                     ARTEEFKQRYRNGESLDKLLPEAFAVVREGGRRALGMRHFDVQMIGGMALHYGKIAEM
                     RTGEGKTLVATLPVYLNAIAGKGVHVVTVNDYLASRDAAWMGKLYNFLGLSYGVNLSQ
                     MPHDAKQQAYAADITYGTNNEFGFDYLRDNMVYTAAERVQRSLNYAVVDEVDSILIDE
                     ARTPLIISGQAEDSVQLYSAINAVALKLVRQKTEEGEGDYWVDEKSQQVLMSEAGHEH
                     AEALLTEAGLLVAGSLYDAANITLVHHLYASLRAQSLFHRDQHYVVRDGEIVIVDEFT
                     GRMMAGRRWSEGLHQAVEAKEGVPIQKENQTLASITFQNYFRMYAKLSGMTGTADTEA
                     FEFNQIYGLETVVIPTNNAMIRQDAMDKVYRTAREKYQAVIEDIQECQKRGQPVLVGT
                     TSIENSELISGLLDKEKLPHQVLNAKQHEREAHIIAQAGRPNMITIATNMAGRGTDIV
                     LGGNPEADLLKVEEDDSLSMEQKAERIALLKAEWQKVHDQVLASGGLHIIGTERHESR
                     RVDNQLRGRSGRQGDPGSSRFYLSLEDSLLRIFASDRVSAIMERLKMPEGEAIEHPWV
                     TRAIE"
     assembly_gap    3934..3943
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3944..4799
                     /locus_tag="PKW44_06025"
     CDS             <3944..4799
                     /locus_tag="PKW44_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020163714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HQR51176.1"
                     /translation="PLVTRAPEPAQRKVEPRNFDIRKQLLEYDDVANDQRKVIYQQRN
                     ELLEATDIADTIVAMRNDVITDFIAQHIPPGSMEEQWEVPALEKSLQAEIGLELPIQQ
                     WLEKEPDLHEETLRERVITAADEAYQSKVEQAGADVMRQFESAVMLQSLDNHWREHLA
                     ALDHLRQGIHLRSYAQKNPKQEYKREAFELFSNLLDTIKREVTQITMMVQIRSEADVE
                     AVEKPAELENVQYQHADYDQALADEAAAGEAAKAEPFERTGLKVGRNDLCPCGSGKKY
                     KQCHGRLT"
     gene            4827..5009
                     /locus_tag="PKW44_06030"
     CDS             4827..5009
                     /locus_tag="PKW44_06030"
                     /inference="COORDINATES: protein motif:HMM:NF018630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HQR51177.1"
                     /translation="MLTIPSPCIGNCRINLKAGLCEGCYRTMDEITAWPKLRDDQRHQ
                     VILVVEQRKTKMARPE"
     gene            5059..>5997
                     /locus_tag="PKW44_06035"
     CDS             5059..>5997
                     /locus_tag="PKW44_06035"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HQR51178.1"
                     /translation="MTDLSRYFAPDGPLAESISGYRLRAQQLEMAQAIAQAIHDNSVL
                     VAEAGTGTGKTFAYLVPALLSGGKVIISTGTKTLQDQLFNRDLPAVRDALKVPVTVAM
                     LKGRANYVCHFHLDRAAKEGRFMNREDARYVHVIRNFAETSASGDKSELPEVPENATI
                     WPAVTSTRDNCIGQECAHYKECFVMEARKRAMAADVVVVNHHLFFADVMLRDEGVSEL
                     LPAANTVIFDEAHQLPETAGLFFGEDISTSQIMELARDAHAEYLTSAKDCAALPEATT
                     ALEKAVRDFRLVFGLEGARMPVQRALERRGFSEAYDG"
BASE COUNT         1254 a         1874 c         1822 g         1037 t
ORIGIN      
        1 accacatggt catcatcggc ctcttcctcg gtggcctgat tccctacctg ttcggtgcca
       61 tggcgatgga ggcggtcggc cgcgccgcca gttccgtggt ggtggaagtg cgtcgccagg
      121 tcaaggaaat tccgggcatc atgaatggca ccgaaaagcc ggactattcc cgtgcggtgg
      181 acatgctgac caagtctgcc atcaaggaaa tgatcgtgcc ttcactgctg ccggtgctgg
      241 tgcccattct ggtgggcaag ttcctcggcg cgcaggcgct gggtggcctg ctgatcggca
      301 ccatcgtcac cggcctgttc gtggcgattt ccatgacggt cggcggcggt gcgtgggata
      361 acgccaagaa gcacatcgaa gacggccacc acggcggcaa gggttccgaa gcgcacaagg
      421 cttcggtgac cggcgatacc gtcggcgatc catacaagga taccgccggc cctgcgatca
      481 cctcgctgat caagatcatc aacattgtcg cgctgctgat cgtctcgatc ctgtaagcaa
      541 tagcagtcat caaaaagccc gccggcgcat cagcgctggc gggctttttt ctgccgcttt
      601 tgcgggctcg ggaatttgtc tgtttcagcc ccgttttgcc agccgttcca gtgccgttcg
      661 cagaggagaa tcgggcaatt tttccgccag tttctccagg ctggccgagg ccttgctact
      721 gaggtggcgc gctacgccag cactggcgcg ggcttgagat ttgacttgca cttcgacccg
      781 aattgaagta acttccagac ctttgatttg caggcttttg agcaagttcg gcgccagtaa
      841 tttgagcttc gccgcgactg cgccgttatc ggcaaagacg gtgatgcgcc ggtgctgaag
      901 tccgcctgca cgagtaaatg gctgcagcgg ttgcggcacg acctcggacc aggttttctg
      961 cagctcggca agtctgccgg tctggttagc caaagcctgc agctcggcat tgtccttgaa
     1021 cagcgcgtca agacgccgca tgccgggagc cttggggcct gcttcgcgag aaacgggttc
     1081 ttgaaaaggg gaaatgcatg aatatcattt tcgtatccaa caacatggcg aaggccagga
     1141 cgctgtcgct acggcaggct ttggcgctgc tggcagggct gctgatcgtc ccgcccctgc
     1201 tgactgccgc cctcattttg ccacagccgg gcgccgagca acagggcatg caggcattgc
     1261 tgccgatgcg cctgattcag gcggtgcgcg acaattcgca ggcacacctc gatgcgctgg
     1321 cgatgcaact gggccagatc caggcgcgcg tgatgcgatt ggatgccctg agcgagcgcc
     1381 tcgccaaatt ggcgggggtc gatgataagg aaattgaaag cgactcacag acgccggggc
     1441 agggcggccc gctgctgcag gcccgcagtc ttaccgccga tgaaatcggc cggcagatgg
     1501 cggaattgac gcgccaactg gaagagcgga acgaccgcct gggtgtgctg gaagccctgc
     1561 tgctgcaaca gaacctcaag cacaccacga ttccgaacag ctctccggtc gaggcggcct
     1621 acaactcttc cagttttggc tggcgcaatg atccctttac cggccagatg gcgtttcatg
     1681 aagggttgga ctttaccgca gaaaagggca cccccatcta cgctgcggcg gatggcatcg
     1741 tcagccaggc cgagagtacg ccggattatg gtaatatcgt caaagtcgat catggctccg
     1801 gccttgagac gcgatacgcg catgcctcgc gcattctggt caagccgggt gagcgggtgg
     1861 agaaagggca attgatcgcc gaggttggca ataccgggcg ttccaccggc gcgcacctgc
     1921 atttcgaggt gcgcctcaac ggcgtggcgc tggatccgcg aaagtacctg caaaatcgta
     1981 atagttagca atttccccat ttaaatttca gaaagttccg cccaggcgga aagtcggcct
     2041 ccccctatgt tatccacggt attcaagaaa attttcggca gtcgcaacga gcggctgatc
     2101 aagcaatatg ctcaggtggt gcgcaccatc aacgcgctgg aagaagccac ccaggcgctt
     2161 tccgacgacc agctgcgcgc caggacagag gagttcaagc agcgttaccg gaatggcgaa
     2221 tcgctcgaca agctgctgcc ggaggctttt gctgtggtgc gcgaaggcgg ccgccgtgcc
     2281 ttgggcatgc gccacttcga cgttcagatg attggcggca tggcgctgca ctacggcaag
     2341 atcgccgaaa tgcgcaccgg tgaaggcaag accttggtgg cgacgctgcc ggtctacctc
     2401 aacgctatcg ccggcaaggg cgtgcacgtg gtgacggtga acgactatct ggccagccgc
     2461 gatgccgcct ggatgggcaa gctgtacaac ttcctcggcc tgtcctatgg cgtcaacctg
     2521 tcgcagatgc cgcatgacgc caagcagcag gcctatgccg ccgacatcac ctacggcacc
     2581 aacaacgaat ttggcttcga ctacttgcgc gacaacatgg tctacactgc ggccgagcgc
     2641 gtgcagcgca gcctcaacta tgccgtggtc gacgaagtgg actccatcct catcgacgag
     2701 gcgcgtacgc cgctgatcat ttccggccag gccgaagaca gcgtgcaatt gtattccgcc
     2761 atcaacgccg ttgcactcaa gctcgttcgc cagaagaccg aggagggcga aggcgactac
     2821 tgggtggatg aaaaatcgca gcaggtgctg atgtccgaag ccgggcacga gcatgccgag
     2881 gcgctgctga cggaagccgg cctgctcgtg gcgggcagcc tgtacgatgc cgccaacatc
     2941 accctggtgc accatctgta tgcttcgctg cgcgcgcagt cgctgttcca ccgcgaccag
     3001 cactacgtgg tgcgcgatgg cgagatcgtc attgtcgacg aattcaccgg ccgcatgatg
     3061 gccggccgcc gttggtcgga aggcctgcac caggcggtgg aagccaagga aggcgtgccg
     3121 atccagaagg agaaccagac actggcctcc atcactttcc agaactattt ccgcatgtac
     3181 gccaagctgt ccggcatgac cggcaccgcc gacaccgaag ccttcgagtt caaccagatc
     3241 tacggcctgg aaacggtggt gatccccacc aacaacgcca tgatccgcca ggatgcgatg
     3301 gacaaggtct accgcaccgc gcgcgaaaaa taccaggcgg tgatcgagga catccaggaa
     3361 tgccagaagc gcggccagcc ggtgctggtc ggtactacct ccattgagaa ctccgaactg
     3421 atttccgggt tgctggacaa ggaaaagctg ccgcatcagg tactcaacgc caagcagcac
     3481 gagcgcgagg cgcacatcat cgcccaggcc ggcaggccga acatgatcac catcgccacc
     3541 aacatggccg gccgtggtac cgacatcgtg ctgggcggca atccggaggc cgatctcctc
     3601 aaggttgagg aggacgacag cctgtcgatg gaacagaagg ccgaacgcat cgccttgctc
     3661 aaggccgaat ggcaaaaggt acatgaccag gtgctggcct ccggcggcct gcatatcatc
     3721 ggcaccgagc gccacgaatc ccgccgggtc gacaaccagc tgcggggacg ctccggccgc
     3781 cagggcgacc ccggctccag ccgcttctac ctgtcgctgg aagattctct gctgcgcatt
     3841 tttgcctccg accgcgtctc tgccatcatg gagcggctga agatgccgga aggcgaggcc
     3901 atcgagcatc cctgggtgac gcgcgccatc gaannnnnnn nnncccgctc gtcacccgcg
     3961 cgcccgaacc ggcacagcgc aaggtcgaac cccgcaactt cgacatccgc aaacagttgc
     4021 tggaatacga cgacgtcgcc aacgaccagc gcaaggtcat ctaccagcaa cgcaatgaat
     4081 tgctggaagc caccgatatt gccgacacca tcgtcgccat gcgcaacgac gtcattaccg
     4141 acttcatcgc acagcacatc ccgcccggca gcatggaaga acagtgggaa gtgccggcgc
     4201 tggagaaatc gctgcaggcc gaaatcggtc tcgagctgcc tatccagcaa tggctggaaa
     4261 aggaacccga cctacacgag gaaaccctgc gcgagcgtgt tatcacggcc gccgacgagg
     4321 cttaccagag caaggtggaa caggccggtg ccgatgtcat gcgccagttc gagagcgccg
     4381 tcatgctgca aagcctagat aaccactggc gtgaacacct ggccgcgctc gaccacctgc
     4441 gccagggcat ccacctgcgc tcctacgcgc agaagaatcc caagcaggaa tacaagcgcg
     4501 aggcgttcga gctgttctcc aacctgctgg acaccatcaa gcgcgaagtg acgcagatca
     4561 ccatgatggt gcagatccgc agcgaagccg acgtcgaggc ggtggaaaag cccgccgaac
     4621 tggaaaacgt gcagtaccag cacgccgatt acgaccaggc gctggcggat gaggcggcgg
     4681 caggcgaagc ggcgaaggcg gagccgttcg agcgcactgg cctcaaggtt ggccgcaacg
     4741 atctgtgccc ctgcggttcc ggcaagaagt acaagcaatg ccacggcagg ctgacctgaa
     4801 caagtttcgc gttggactgg aagcatatgc tgacgatccc gtctccctgc atcggcaact
     4861 gccgcatcaa cctcaaggca gggttgtgcg aaggctgcta ccgcaccatg gacgaaatca
     4921 cggcctggcc caagctgcgc gatgaccagc gccaccaggt gatcctggta gtggagcagc
     4981 gcaagaccaa aatggccaga cccgaatgac aagcgccggg gaaacccggc atttgcattt
     5041 gggtggctgc tgccctgaat gaccgacctc tcccgctatt tcgctcccga tggcccgctg
     5101 gccgaatcca tctccggcta ccggctgcgc gcgcagcagc tggaaatggc gcaggccatc
     5161 gcccaggcga tccatgacaa cagcgtgctg gtggccgaag ccggcaccgg caccggcaag
     5221 accttcgcct acctcgtccc cgcgctgctg tccggcggca aggtcatcat ctccaccggc
     5281 accaagaccc tgcaggacca gctgttcaac cgcgacctgc cggcggtgcg cgatgccctg
     5341 aaagtcccgg ttaccgtggc catgctcaag ggccgcgcca actacgtctg ccacttccac
     5401 ctcgaccggg cggcgaaaga ggggcgcttc atgaatcgcg aagacgcccg ctacgtgcac
     5461 gtcatccgca atttcgccga aaccagcgcc agcggcgaca agagcgagct gccagaagtg
     5521 ccggaaaacg ccaccatctg gccggcggtg acctccacgc gcgacaactg tatcggccag
     5581 gaatgcgcgc attacaagga atgcttcgtg atggaggcgc gcaagcgggc aatggcggcc
     5641 gacgtggtgg tagtcaacca tcacctgttc ttcgccgacg tcatgctgcg cgacgaaggc
     5701 gtttccgaac tgctgccggc ggccaacacg gtgatcttcg acgaagccca ccagctgccg
     5761 gaaaccgccg gcctgttctt cggcgaggat atctccacca gccagatcat ggagctggcg
     5821 cgcgatgctc acgccgaata ccttaccagc gccaaggatt gcgcggcgct gcccgaggcc
     5881 accaccgcgc tggaaaaagc cgtgcgcgat ttccgcctgg tgtttggcct ggaaggcgct
     5941 cgtatgccgg tgcagcgggc gctggaacgc cgcggtttca gcgaggctta cgatggc
//
LOCUS       DAOMFA010000099         5886 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_1738_length_5886_cov_5.135140, whole genome shotgun sequence.
ACCESSION   DAOMFA010000099 DAOMFA010000000
VERSION     DAOMFA010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 5886)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5886)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5886
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1738_length_5886_cov_5.135140"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..241)
                     /locus_tag="PKW44_06040"
     CDS             complement(<1..241)
                     /locus_tag="PKW44_06040"
                     /inference="COORDINATES: protein motif:HMM:NF014066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HQR51179.1"
                     /translation="MSRCILKPLLGAMLIGLLASVPFSHAEDIGLLWKAESPAGKTSY
                     LFGTMHSDDARINDFSPALLKAIADSEIFMAEVLPS"
     gene            complement(330..1853)
                     /locus_tag="PKW44_06045"
     CDS             complement(330..1853)
                     /locus_tag="PKW44_06045"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HQR51180.1"
                     /translation="MWQSVNAWQGGIAQLALLTAIVGALLLRFRNADRRSIGLTLGVF
                     AASLAGIFVSGMMASTGFPALGAWLHEIFMILQGVAVIRLGGMFVFRLLLPLVRLQSP
                     RILEDIVVFITYLGWGFVRLHDAGLDLSGIITTSAVITAVIAFSMQDTLGNILGGLAL
                     QLDNSIEVGDWIKIEDIIGKVVDIRWRHTAIETRNWETVVVPNSQLMKTKFSVLGRHG
                     EDPVQWRRWIWFNVGYQTSPAHVIETVQRALHDTDIRNVARQPQAHCVLMEFDQSYGR
                     YAVRYWLTDLMADDLTDSEVRDHVYAALQRAGIRLALPEHSVHMTKESEKHEQARQMR
                     RIQERMEALRKVELFNGFTEEELLDIAQRLKYAPFAKGDVITRQGAVAHWLYMLSEGE
                     AEVYLETPGQERRKLSTLHPGNFFGEMGLMTGAPRTATVVAATDAECYLLDKTSFETV
                     LSKRPELAEEISKTLVSRRFGLDSLQQDVDADTRAQQMAQQHQDILARIRHFFGLGQ"
     gene            2052..2123
                     /gene="pqqA"
                     /locus_tag="PKW44_06050"
     CDS             2052..2123
                     /gene="pqqA"
                     /locus_tag="PKW44_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013440845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone precursor peptide PqqA"
                     /protein_id="HQR51181.1"
                     /translation="MWTKPEVTEMRFGFEVTMYVCNR"
     gene            2210..3124
                     /gene="pqqB"
                     /locus_tag="PKW44_06055"
     CDS             2210..3124
                     /gene="pqqB"
                     /locus_tag="PKW44_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008059648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis protein
                     PqqB"
                     /protein_id="HQR51182.1"
                     /translation="MHIHVLGAGAGGGFPQWNCNCHNCDGLRKGTIKARPRTQSSICV
                     SGDGANWVLFNASPDVLKQFQEFPPLQPARAIRDTGMVGIVLIDAQIDHTTGLLMLRE
                     GTVKREIFCTEMVRNDLTSGNPLFNILDHYCGVNWHNVPIDGSSFEIPGAANLRFTTV
                     ALKSKAPPYSPHRYDSHDGDTIGVLVEDTSNGKKLFYAPGLGEIEPHLPPLFAQADCI
                     MVDGTFWTNTEMIELGLMDKTARDIGHLPQSGEGGMMEVLSAYPKARRVLIHINNTNP
                     ILNEESPQRRELEATGIEVSYDGMDIIL"
     gene            3137..3895
                     /gene="pqqC"
                     /locus_tag="PKW44_06060"
     CDS             3137..3895
                     /gene="pqqC"
                     /locus_tag="PKW44_06060"
                     /EC_number="1.3.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline-quinone synthase PqqC"
                     /protein_id="HQR51183.1"
                     /translation="MSDNLQPWSREEFEQKLRERGRGYHIHHPFHVLMYEGKLSQKQM
                     QCWVANRYYYQIAIPIKDAAILSNCWDRELRRGWIQRIFDHDGSGHINEGGEGGIEAW
                     IQLGAAVGLTREEVTSLKLVSPAARFAVDAYINFARQRPWQEAVCSSLTELFAPHIHQ
                     QRIDAWPEKYPWVKPEGMQYFKNRLTQARRDVKQGLDITLDYFGKSREMQERACEILQ
                     FKLDVLWALADAIMLQCCDVKIEGGTQYVRQDQV"
     gene            3873..4157
                     /gene="pqqD"
                     /locus_tag="PKW44_06065"
     CDS             3873..4157
                     /gene="pqqD"
                     /locus_tag="PKW44_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009852447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis peptide
                     chaperone PqqD"
                     /protein_id="HQR51184.1"
                     /translation="MSDKIKSDAVFCLARHYRFQWEEAQGGYVLLFPEGMIKLNGGAG
                     EVIKRVDGKKTVGEIVADCKAAFPDVDGLEVDVLAMLELAHEKSWIALKE"
     gene            4170..5354
                     /gene="pqqE"
                     /locus_tag="PKW44_06070"
     CDS             4170..5354
                     /gene="pqqE"
                     /locus_tag="PKW44_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018149 - peptide cross-linking [Evidence
                     IEA];
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis protein
                     PqqE"
                     /protein_id="HQR51185.1"
                     /translation="MAQQNNGAVLHHAPKPFWLLAEITYRCPLHCAFCYNPTDYVTHT
                     QNELSTEQWIKVLRDARKMGALQLGISGGEPLLRDDIEEIVAEAGSLGYYSNLITSGV
                     GLTEKRIAEFKRGGLDHIQLSMHDITKEINNFITDTNTFELKKKVAAMIKEYDYPMVL
                     NVVIHRYNIDHIGEILEMAEQIGANYVELANTQYYGWSLLNRAQLMPTREQLDKAEAI
                     TNAFRKKVGSKMQIFFVVPDYYADRPKKCMNGWGEVFMIVTANGDVLPCHSARSLPGL
                     TFPNVKDGTDVEWIWNESPAFNKYRGDDWMKEPCRSCPEKEKDLGGCRCQAYLLTGDA
                     EIADPVCSLSPAHGVILQAIEDAKNPQLAGQPILFRNDKNSRRLVEGELKEVASPFHA
                     LP"
     gene            5469..>5886
                     /locus_tag="PKW44_06075"
     CDS             5469..>5886
                     /locus_tag="PKW44_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQR51186.1"
                     /translation="MPSPARFAIILAALAALAPFAIDTYLPAFPVLETALGGSALELQ
                     QSLTFYLLPYALMTLWHGAISDEIGRLAAIRWGLSVFILASVGCAFAPNVETLWFFRA
                     LQGISGGAGHVVARAMVCDLFQGAQAQRVMATVLMLF"
BASE COUNT         1217 a         1688 c         1775 g         1206 t
ORIGIN      
        1 tcgacggcag cacctcggcc atgaaaatct cgctgtcggc gatggctttc agcagcgccg
       61 gggagaaatc gttgatgcgc gcatcgtcgg aatgcatggt gccgaacagg tagctggttt
      121 tgccggcggg ggattcggct ttccacagca ggccgatgtc ttcggcgtgt gaaaacggca
      181 cggatgccag caggccaatc agcatggcgc ctagcagggg tttcagaatg cagcgtgaca
      241 tcatacttcc cggtctgggt gttcagtggt tcgacacgcc ggctacggtt ccggttcagc
      301 agggccacag tttcacatgg gctgtggcgc tactggccca ggccgaaaaa atgccggatc
      361 cgtgccagga tatcctggtg ctgctgcgcc atctgttggg cgcgcgtgtc ggcgtccaca
      421 tcctgctgca ggctgtccag gccaaaacgg cggctgacca gagtcttgga aatctcctcg
      481 gcgagttccg ggcgcttgct cagcaccgtc tcgaacgagg tcttgtccag caggtagcac
      541 tcggcatccg tggcggccac gacggtggcg gtacgcgggg cgcctgtcat caagcccatt
      601 tcgccgaaga agttgcctgg gtgcagggtg gaaagcttgc ggcgctcctg gccgggggtt
      661 tccagataaa cctcagcctc accttcggaa agcatgtaca gccagtgtgc cacggcgccc
      721 tggcgcgtga tgacgtcgcc cttggcgaaa ggggcatatt tcaggcgttg cgcgatgtcg
      781 agcagttcct cctcggtgaa gccgttaaac agttccacct tgcgcagcgc ttccatgcgt
      841 tcctgaatgc ggcgcatttg cctggcctgc tcgtgctttt cgctttcctt ggtcatgtgc
      901 acgctgtgct caggcagcgc gaggcggatg ccagcacgtt gcagggcggc gtagacatgg
      961 tcgcgcactt cggagtcggt caagtcgtct gccatcaggt cggtcaacca gtaacgcacg
     1021 gcgtagcgcc cgtagctctg gtcgaattcc atcaatacgc aatgggcttg cggctgcctg
     1081 gcgacgttgc ggatgtcggt gtcgtgcagg gcacgctgca cggtttcgat gacatgtgcc
     1141 ggggaggtct ggtagccgac gttgaaccag atccaccgac gccactgcac gggatcttcg
     1201 ccatggcgcc cgagcacgga gaacttggtt ttcatcagct ggctgttggg gactaccacg
     1261 gtttcccagt tacgggtctc gatggcggta tgccgccacc ggatgtcgac cactttgccg
     1321 atgatatcct ctatcttgat ccagtcgccg acttcgatgg agttgtccag ctgcagcgcc
     1381 agcccgccca ggatgttgcc cagggtatcc tgcatggaaa aggcgatcac ggcggtgatg
     1441 acggccgagg tggtgatgat gccggacagg tccagcccgg catcatgcag ccggacgaag
     1501 ccccagccca ggtaggtgat gaagacgacg atatcttcca ggatgcgcgg cgattgcagc
     1561 cttaccaggg gcagcagcag gcggaagacg aacatgccgc ccagacggat gacggcaaca
     1621 ccctgcagga tcatgaagat ttcgtgcagc catgcgccca aggcgggaaa tccggtcgag
     1681 gccatcatgc cgctgacgaa tatgcctgcc aagctggcgg cgaatactcc cagggtaagt
     1741 ccaatggaac gccgatccgc attgcggaag cgtagcagca gtgcgccgac gatagcggtg
     1801 agcagggcca gttgggcaat gcccccctgc caggcattga cactttgcca catggattga
     1861 agcaggttca tcggaccatt ttaacggtca aggcccgttg tcgccagtat cggagagctc
     1921 atgataatca tgagcgctat ttactgattt ttgcgctgtt ggctgccggg tgtccggcgc
     1981 aaaatgcatc tcgaacgtgc tgcatgtgta gtgtgttcga ccaaccaaaa actttaacca
     2041 ggagattcaa catgtggacc aagcctgagg tgaccgaaat gcgttttggc ttcgaagtga
     2101 ccatgtacgt ctgcaaccgc taagttgctg cgtcatggaa ggccgccgag tgcggccttc
     2161 ttgccctgaa taatagggac tttcctgtcc ttttactggt tttaatttca tgcatattca
     2221 cgttctaggt gccggtgccg gcggcggttt cccccagtgg aactgcaatt gccataactg
     2281 cgacggcctg cgcaagggca ccatcaaagc gcgtccgcgc acccagtctt ccatttgcgt
     2341 cagtggtgat ggtgccaact gggtattgtt caatgcttca cccgatgtgc tcaagcagtt
     2401 ccaggagttc ccccccctgc agccggcgcg tgccattcgc gacaccggca tggtcggcat
     2461 cgtgctgatc gacgcgcaga tcgaccatac taccggcctg ctgatgttgc gcgagggaac
     2521 ggtcaagcgc gaaatcttct gtacagagat ggttaggaac gacctgacca gcggtaatcc
     2581 gctgttcaac atcctggacc attactgcgg cgttaactgg cataacgtgc cgatcgatgg
     2641 ctccagcttc gaaattcctg gcgccgccaa tctcagattc accacggtgg cattgaagag
     2701 caaggcgccg ccgtattcgc cgcatcgcta cgacagccat gacggggaca ccatcggcgt
     2761 gctggtcgag gacaccagca acggcaagaa gctgttctac gcgccgggcc tcggcgagat
     2821 cgagccgcac ctgccgccgt tgttcgccca agccgactgc atcatggtgg acggcacctt
     2881 ctggaccaac accgaaatga tcgaactcgg cctgatggat aaaacggccc gcgacatcgg
     2941 ccacctgccg caatccggcg aaggcggcat gatggaagtg ctgtcggcct accccaaggc
     3001 gcgccgcgtg ctgatccata tcaacaacac caaccccatc ctgaacgagg aatccccgca
     3061 gcgccgcgaa ctggaagcga ccggcatcga ggtttcctac gacggcatgg acatcattct
     3121 gtaaatggag caagaaatga gcgataacct gcaaccctgg agccgtgaag aatttgaaca
     3181 gaagctgcgc gagcgcggcc gtggctatca tatccatcac ccgtttcacg tgctgatgta
     3241 cgaaggcaag ctttcccaga agcagatgca gtgctgggtg gccaaccgtt actattacca
     3301 gatcgccatc ccgatcaaag atgcggcgat cctttccaac tgctgggatc gcgaactgcg
     3361 tcgcggctgg attcagcgta ttttcgacca tgacggcagc ggtcatatca acgagggggg
     3421 cgaaggcggc atcgaggctt ggatccagtt gggtgcggca gtcggcctca cgcgcgagga
     3481 agtcacttcg ctcaagctgg tatcgccggc agcgcggttc gcagtggatg cctacatcaa
     3541 ctttgcacgc cagcgtccgt ggcaggaagc cgtttgttcc tcgttgaccg agctgttcgc
     3601 gccgcatatc caccagcagc gcatcgatgc ctggccggaa aagtacccct gggtgaagcc
     3661 cgaaggtatg cagtatttca agaaccgcct gacgcaggcg cgtcgcgatg tgaaacaggg
     3721 gctggacatt acgctggatt acttcggcaa gtcgcgcgaa atgcaggagc gcgcctgcga
     3781 gatcctgcag ttcaaactgg acgtgctgtg ggcgctggcc gacgccatca tgctgcagtg
     3841 ctgtgacgtg aagatcgagg gtggcacgca atatgtccga caagatcaag tctgatgccg
     3901 tattctgcct cgcgcgccac taccgatttc agtgggagga agcgcagggc ggctacgtgc
     3961 tgctgtttcc cgaaggcatg atcaaactca acggcggggc gggcgaggtc atcaagcgcg
     4021 tagacggaaa aaagacggtg ggggaaattg tcgctgattg caaggccgct tttccggatg
     4081 ttgatggctt ggaagtcgac gtgctcgcca tgctggaact tgcgcacgag aaatcctgga
     4141 ttgcattgaa ggaataaaca gaggtcatta tggctcaaca aaataacggt gccgtactcc
     4201 atcacgcccc caagccgttc tggctgctgg cggagatcac ctatcgctgt ccgctgcact
     4261 gtgcgttctg ctacaacccg acggattacg tcacgcacac gcagaacgag ctttccaccg
     4321 aacagtggat caaggtgctg cgcgacgcgc gcaagatggg ggcactgcag ctcggcatct
     4381 ccggcggcga accgctgctg cgcgatgaca tcgaagagat cgtggccgag gcgggcagtc
     4441 tgggctacta cagtaatctg atcacttccg gtgttgggct gaccgaaaag cgcatcgctg
     4501 aattcaagcg tggcggactg gatcatatcc agctttccat gcacgatatc accaaggaaa
     4561 tcaataactt catcactgac accaacactt tcgagctgaa aaagaaggtg gcagccatga
     4621 tcaaggaata cgattacccc atggtgctca atgtggtgat ccatcgctat aacattgacc
     4681 atatcggcga aatcctcgaa atggccgagc agatcggcgc caattatgtc gaattggcta
     4741 atacccagta ttacggctgg tctctgctca accgcgcaca actgatgccc acccgcgagc
     4801 aattggacaa agctgaggcc atcaccaatg cattccgtaa aaaggtcggc agcaagatgc
     4861 agatattctt cgtggtgccg gactattatg ccgaccgccc gaagaagtgc atgaacggct
     4921 ggggcgaggt gttcatgatc gtcaccgcca acggcgacgt gctaccgtgc cattccgccc
     4981 gttcgctgcc tgggctgaca ttccccaacg tcaaggacgg taccgatgtc gagtggatat
     5041 ggaacgaatc gcctgccttc aacaaatatc gcggcgacga ctggatgaaa gagccgtgcc
     5101 gttcctgtcc ggaaaaggaa aaagacctcg ggggctgccg ctgccaggcc tatctgctga
     5161 ccggcgacgc cgaaatcgct gatccggtgt gcagcctgtc gccggcgcac ggtgtcatcc
     5221 tgcaggccat cgaggatgcc aagaacccgc agttggccgg gcagcctatc ctgttccgca
     5281 acgacaagaa ctcgcgccgg ctggtcgagg gtgagttgaa agaagtcgcc tcgccgttcc
     5341 acgcactgcc atagatcaga gtcgtagatc aaaccacccc ggcgtagccg ccggggtggt
     5401 tgcttgttgg aaggctgccc tagttgtgcg tcagtcaatg acaccccatc ccgtgccata
     5461 atccgtctat gccatctccc gctcgttttg ccattattct ggcggccctc gccgcgttgg
     5521 cgccatttgc catcgacact tatctgccgg ccttcccggt gctggaaacg gcgctcggcg
     5581 gcagcgcgct ggagttacag caaagcctga cgttttacct gctgccgtac gcgttgatga
     5641 cgctttggca tggcgccatc tccgacgaaa tcggccggtt ggcggcgatt cgatggggtt
     5701 tgagtgtgtt catcctggct tcggtgggct gtgcgtttgc accgaacgtg gagacattgt
     5761 ggttctttcg cgctttacaa ggcatctcgg gtggcgcagg ccatgtggtg gcgcgcgcca
     5821 tggtgtgcga tttattccag ggcgcacagg cacagcgcgt gatggcaact gtgctgatgt
     5881 tgttcg
//
LOCUS       DAOMFA010000100         5879 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_1743_length_5879_cov_6.308551, whole genome shotgun sequence.
ACCESSION   DAOMFA010000100 DAOMFA010000000
VERSION     DAOMFA010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 5879)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5879)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5879
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1743_length_5879_cov_6.308551"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1120
                     /gene="glgB"
                     /locus_tag="PKW44_06080"
     CDS             <1..1120
                     /gene="glgB"
                     /locus_tag="PKW44_06080"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HQR51187.1"
                     /translation="GTALYEHEDPRLGEHQDWGKLIFNYGRNEVRNFLLANAHYWMHE
                     FHIDGLRVDAVASMLYLDYSRKAGEWLPNKFGGRENLEAIDFMKELNVMVHEDFPGAL
                     TIAEESTAWPMISRPVYLGGLGFSMKWNMGWMNDTLAYFKQDPVHRRYHHNQLTFGQL
                     YAYTENFVLPFSHDEVVHGKRSLLDKMPGDGWQKFANLRLLLTYQMTYPGKKLNFMGN
                     EIGQGREWNATASLDWHLLDTHWHQGVHQLTGDLGKLYLGTPALHELDFTHEGFAWID
                     CHDADQSIISYQRRARNGSCVLVLLNFTPVPREGYRIGVPQAGRYIELLNSDAACYGG
                     GNLGNGAGLQSEDRPWMGYPHSVVVTLPPLAGLILKLE"
     gene            1139..2788
                     /gene="pgi"
                     /locus_tag="PKW44_06085"
     CDS             1139..2788
                     /gene="pgi"
                     /locus_tag="PKW44_06085"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018286888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HQR51188.1"
                     /translation="MSKLTTSEIWQTLIKHQQEIAPKHMREMFADDPARFIRFSLQFN
                     DILLDYSKNRITEETLTRLLQLARESGVEEWRDRMFSGEKINFTEQRAVLHTALRNRS
                     KRPVLVDGKDVMPDIRRVLAHMRQFTERVRSGEWKGYTGKAITDVVNIGIGGSDLGPA
                     MVCKALHPYSRADLRAHFVSNVDGAHLSIVLQQCDPETTLFVVASKTFTTQETLINAH
                     SARRWFLEKSGSESHIAKHFVALSTNAEEVKKFGIDTANMFEFWDWVGGRYSLWSAIG
                     LSIALHVGMDAFEQLQQGAFEVDEHFRSAPLEQNMPVILAMIGIWYNNFFGAQTHATL
                     PYDESIGPYPAYFQQGDMESNGKAIDREGRRVDYSTGPIIWGEPGTNGQHAFFQLIHQ
                     GSKMVPCDFLMPIETHYPIGEHHQILFSNFLAQTKALMQGKSEPDARTELEAAGIKGD
                     ALEKLLPHKIFRGNRPTNSILFNKLDPKTLGSLIAIYEHKIFVQGIVWHINSFDQWGV
                     ELGKQLAQAILPQLQDNKPISENDSSTNGLINHCKKLRQNS"
     gene            2785..3249
                     /locus_tag="PKW44_06090"
     CDS             2785..3249
                     /locus_tag="PKW44_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MEKHLA domain-containing protein"
                     /protein_id="HQR51189.1"
                     /translation="MSNYPWLVRHVRMLRVSYLHWVGKHLVEPSLDDEAAVTALELLP
                     FAVVSHDTGHDPVFNYANQAALQLFEMSWEQFTVLPSRLSAEPMLREERERLLQRVAE
                     SGFIDDYRSIRISASGKRFLICNATVWNLLDDQGRPYGQAALLREWQALNNS"
     gene            complement(3265..3663)
                     /locus_tag="PKW44_06095"
     CDS             complement(3265..3663)
                     /locus_tag="PKW44_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HQR51190.1"
                     /translation="MDTEQGTQKVRLDKWLWAARFFKTRSLAADAVDSGKVHVDGDRA
                     KPAKEVRLGAVLRIRRGSEEMEVEVRGLSMQRKGAPEAVLLYEETEASRARREEAAIT
                     READHAQRDRGLGRPTKRQRRDIKRFTGNW"
     gene            complement(3704..5035)
                     /locus_tag="PKW44_06100"
     CDS             complement(3704..5035)
                     /locus_tag="PKW44_06100"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HQR51191.1"
                     /translation="MGNNKHCHDWPLHRVLIFRIGATALVMAVAAAAIVYWIERQKLH
                     DTAVELATRRAAEFVAEAGNVFTAPGAAEKGEVQTRLDRFVREHLPPREGSIVAAMVF
                     DAAGTPVARFARAEFAQRSAAEAFLTAQHRASDGIANARARPAEIGGARVYFVQVPLD
                     ARDGRMLGRLFALYAPSANYVAEFDRRLQRAIIFAIAAVLASAAVLYPVVLRLMRRVT
                     TLSASLLDANLETIQVLGGAIAKRDADTDAHNYRVTIYSVRLAEVVGLDDGDIQALIK
                     GAFLHDVGKIGIHDQILLKPGRLTPEEFVEMKTHVEHGLDIVRRASWLADAAAVVGSH
                     HEKMAGDGYPNGLAGESIPLAARIFAIADVFDALTSRRPYKEPLSCDEAMNILAQGRG
                     SHFDARLFDAFEKIAPALHAQFSSNDDSAAKDTLAQITERYFRQDVGMLLG"
     gene            complement(5111..>5879)
                     /locus_tag="PKW44_06105"
     CDS             complement(5111..>5879)
                     /locus_tag="PKW44_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent protease"
                     /protein_id="HQR51192.1"
                     /translation="VLLESIVDGELMISVHGREVGQINGLTQADLGDLRFGSPVRISA
                     RAYAGEEGVINIDREVEMTGPNHDKGVMILSGWLSATFSQQAPLCLSASLVFEQEYHG
                     VEGDSASCAELYALLSALSGLPLPQGIAVTGALNQHGEVMPIGGINEKIEGYFRACQK
                     LGLDGGHGVLIPARNRQHLLLDDAVVEAVARGEFRIYTISHVLEGVELLTGVSAGSPD
                     ASGNYRQDTVLGRVQRALEGFRKACDTSSHHHDREHG"
BASE COUNT         1239 a         1833 c         1642 g         1165 t
ORIGIN      
        1 cggcaccgcc ctgtatgaac acgaagaccc gcgactgggc gagcatcagg actggggcaa
       61 gctgattttc aactacggcc gcaacgaagt gcgcaacttc ctgctggcca acgcgcacta
      121 ctggatgcac gagttccaca tagacggcct gcgtgtggat gcagtcgcct ccatgctcta
      181 cctggattat tcgcgcaagg ccggcgaatg gctgcccaac aagttcggcg gccgcgaaaa
      241 cctggaagcc atcgacttca tgaaagagtt gaacgtgatg gtgcacgagg atttccccgg
      301 cgcgctcacc atcgccgagg agtccaccgc ctggccgatg atctcgcgtc cggtctatct
      361 gggtggcctg ggcttctcga tgaagtggaa catgggctgg atgaacgaca ctctggccta
      421 tttcaagcag gatccggtgc accggcgcta ccaccacaac cagctcactt tcggccagct
      481 gtatgcttat accgagaatt tcgtgcttcc gttctcgcat gacgaggtgg tacacggcaa
      541 gcgttcgttg ctggacaaga tgccgggcga tggctggcag aagttcgcca atctgcgttt
      601 gttgctgaca taccagatga cttaccccgg caagaagctc aatttcatgg gcaacgaaat
      661 cggtcaggga cgcgaatgga acgccaccgc cagcctggat tggcatctgc tggacacaca
      721 ttggcaccag ggcgtgcacc agttgaccgg tgacttgggc aagctttatc ttggcacgcc
      781 ggcgctacac gagctggatt tcacccatga aggctttgcc tggatcgact gccacgatgc
      841 cgaccagtcc atcatcagct accagcgtcg cgcacgcaat ggatcatgcg tgctggtact
      901 gctaaacttc acccccgtgc cgcgtgaggg ttatcgcatc ggcgtgccgc aggccgggcg
      961 ctatatcgaa ctgctcaaca gcgatgccgc ctgctacggc ggcggcaacc taggcaacgg
     1021 cgcgggcctg caatccgagg atcgaccctg gatgggttat ccgcattccg tggtggtgac
     1081 gctaccgcca ctggctgggc tgatacttaa attggaataa ggacaacaag ctttagtcat
     1141 gtcgaaactg accacttccg aaatctggca aacgctgatc aaacatcagc aggaaatcgc
     1201 gcccaaacac atgcgcgaga tgttcgccga cgatccggca cgttttatca ggttttcgct
     1261 gcaattcaac gatattctgc tggattattc caaaaaccgc attaccgagg aaaccctgac
     1321 ccgactactg cagctggcac gcgagtccgg agtggaggaa tggcgcgacc gcatgttctc
     1381 cggtgaaaaa atcaatttca ccgagcagcg tgcggtgctg cataccgcgc tacgcaaccg
     1441 ttccaagcga ccggtgctgg tggatggcaa ggatgtcatg ccggacatca gacgcgtgct
     1501 ggcgcacatg cgccagttca ccgaaagggt gcgttcgggc gaatggaagg gttacaccgg
     1561 caaggccatc actgacgtcg tcaacatcgg catcggcggc tccgatctcg gtccggccat
     1621 ggtttgcaag gcgttgcatc catactcccg cgccgatctg cgcgcccact tcgtatccaa
     1681 tgtcgatggc gcccatctga gcatcgtcct gcaacaatgc gacccggaaa caacactctt
     1741 cgtcgtcgcg tcaaaaactt tcaccacgca ggaaaccctg atcaacgccc attccgcgcg
     1801 ccgctggttt ctcgaaaaga gcggaagcga atcgcatatc gccaaacact tcgtcgcact
     1861 ttccaccaat gcagaagagg taaagaagtt cggcatcgac actgccaaca tgttcgaatt
     1921 ctgggactgg gtcggcggcc gttattcgct gtggtcggcc atcggcctct ccatcgccct
     1981 ccacgtcggc atggacgcct tcgaacagct gcaacagggc gcgttcgagg tggacgaaca
     2041 tttccgtagc gcgccactgg aacagaacat gccggtcatt cttgccatga tcggtatctg
     2101 gtacaacaac ttctttggcg cgcaaacgca cgccaccctt ccctacgacg aatcgattgg
     2161 gccttacccc gcatacttcc agcaaggcga catggaaagc aacggtaaag ctatcgaccg
     2221 cgaggggcgg cgtgtggatt actccaccgg ccctatcatc tggggcgagc cgggcaccaa
     2281 tggccagcat gcgttcttcc aactcatcca tcagggtagc aagatggttc cctgcgactt
     2341 cctgatgccg atcgaaactc attatcccat aggtgagcac caccagatcc tgttctccaa
     2401 cttcctcgcc cagactaagg cgctgatgca gggcaagagc gagccggatg cgcgcaccga
     2461 actcgaggcg gccggcatta agggggacgc cctggaaaaa ctgctgccac acaaaatttt
     2521 ccgcggcaat cgtcctacca actccatcct gttcaacaag ctggacccga aaaccctggg
     2581 ttcgctgatt gcgatctacg agcacaagat tttcgtccaa ggcatcgtct ggcacatcaa
     2641 ctccttcgat cagtggggcg tagaactggg caaacaactg gcgcaggcta tcctgccgca
     2701 gttgcaggac aacaagccga tatcggagaa cgacagctcc accaacggcc tgatcaacca
     2761 ctgcaagaaa ttgcgccaga attcttgagc aactatcctt ggctggtacg ccatgttcgc
     2821 atgttgcgtg tcagttatct gcactgggtc ggcaagcatc tggtcgagcc gtcactggac
     2881 gacgaagcgg cagtaaccgc gcttgaactg ttgccatttg ccgtcgtttc ccacgatacc
     2941 ggccacgatc cggtattcaa ttacgccaac caggccgcgc tgcaactgtt cgagatgtcg
     3001 tgggaacagt tcaccgtcct gccctcgcgc ctgtccgccg agccgatgct acgtgaagaa
     3061 cgcgaacgcc tgcttcagcg cgtggcggaa agcggtttta tcgacgacta cagaagcatc
     3121 aggatttctg ccagcggaaa gcgcttcctt atctgcaacg ccacggtgtg gaatctgctg
     3181 gacgatcaag gacgaccgta tggccaggcc gcgctgttgc gggagtggca ggcgctgaac
     3241 aatagctaga aaaacagttt gtgtttacca gttaccggta aagcgtttga tatcgcgccg
     3301 ctggcgcttg gtaggacgtc ccaggccacg gtcacgttgc gcgtggtcgg cttcgcgggt
     3361 gatggcagcc tcttcgcgcc gcgcgcgact ggcttcggtt tcctcataca gcagcacggc
     3421 ttccggcgcg cccttccgct gcatggacaa tccgcgcacc tccacttcca tttcctcact
     3481 accgcggcgg atgcgcagca cggcccccag cctgacctct ttcgccggct tggcgcgatc
     3541 gccatcgaca tggaccttgc cgctatccac ggcatcggcg gccaagctgc gcgtcttgaa
     3601 gaagcgcgcc gcccacaacc atttgtcgag gcgtactttt tgcgtgccct gttccgtgtc
     3661 catcatgaat ccggtcgtga gtaacagcgt ttattgatct ggatcagccc aacagcattc
     3721 ccacatcctg ccggaaatag cgctcggtga tttgtgcaag tgtgtctttt gcggcactgt
     3781 catcattgct ggaaaactgt gcatgcaatg cgggcgcgat cttttcgaat gcatcgaaaa
     3841 gtcgcgcatc gaaatggctg ccacgccctt gcgcgagaat gttcatggcc tcgtcgcaac
     3901 tgagcggttc tttgtaagga cgacgcgaag tcagcgcatc gaacacatcg gcaatggcga
     3961 atatccgtgc ggccaaggga atggattctc ccgccagacc attcgggtac ccatcgcccg
     4021 ccatcttttc gtgatggctg ccgaccaccg ctgccgcatc cgcgagccag gaagcgcgcc
     4081 tgacgatatc gagcccatgc tcgacgtggg ttttcatttc cacaaactct tccggcgtca
     4141 ggcgcccggg cttgagcaga atttggtcgt ggatgccaat tttccccacg tcatgcagga
     4201 acgcgccctt gatcaacgcc tgaatatccc cgtcatcgag accgaccacc tcggccaggc
     4261 gtaccgaata gatcgtgacg cggtaattgt gggcatcggt gtcggcgtca cgcttggcga
     4321 tggcgccacc cagcacctga atggtttcga ggttggcatc gagcagactg gcggatagtg
     4381 tagtgacccg gcgcatcaga cgcagcacca ccggataaag tacggcggcc gacgccagca
     4441 ccgctgcaat ggcaaagatg atggcacgct gcaggcgccg atcgaattcc gcgacatagt
     4501 ttgcgctcgg tgcgtacaga gcgaaaagcc gacccagcat gcgcccatcc ctggcatcaa
     4561 gtggcacttg gacgaaatag acacgggccc cgccgatttc cgccggtcgc gcacgcgcgt
     4621 ttgcgatacc gtcagacgca cgatgctgtg ccgtcagaaa agcctctgct gcactgcgct
     4681 gcgcaaactc cgcccgcgcg aagcgcgcaa cgggcgttcc ggcagcgtca aacaccatgg
     4741 ctgccacaat gctgccctct cgtgggggca gatgctcgcg cacaaagcga tccagccgtg
     4801 tctgcacctc acctttttct gctgcaccgg gcgcggtgaa cacattgccg gcttctgcaa
     4861 caaactctgc cgcacgccgc gtggcaagtt ccactgccgt atcatgcagc ttctgccgct
     4921 cgatccagta gactatggca gcggccgcca cggccatcac caacgcagtc gcgccgatac
     4981 ggaagatcag cacccggtgc agcggccaat cgtggcaatg cttgttatta cccatcaagc
     5041 gacccttctg aacgtgcaaa gtggttgaag cgaacacgct tcagaccccc atcatctttt
     5101 tcctgaggca tcagccatgc tcccggtcgt ggtgatggct gctggtgtcg caggccttgc
     5161 gaaaaccttc caaggcgcgc tgcacgcggc ccagcaccgt atcctggcga tagttgccgg
     5221 aagcatccgg cgagcccgcc gacactccgg tcagcagttc gacaccttcc agcacgtggc
     5281 tgatggtgta gatcctgaac tcgccgcgcg ccacggcttc caccactgca tcgtccagca
     5341 acaaatgctg cctgttgcgg gcggggatga ggacgccgtg cccaccatct agccctagct
     5401 tctggcaggc acggaagtaa ccctcgatct tctcgttgat gccgccgatg ggcatgacct
     5461 caccgtgctg gttcaatgcg ccggtgaccg cgatgccctg tggtagcggc agcccggaca
     5521 aggcagacag cagggcgtaa agttcggcac aggaggccga gtcgccctcg acgccgtggt
     5581 attcctgttc aaacaccagc gaggcagaaa ggcatagcgg cgcctgttgc gagaacgtgg
     5641 ccgacaacca gcccgacagg atcatcacgc ccttgtcgtg gttggggccg gtcatttcca
     5701 cctcgcggtc gatgttgatc acaccttctt cgccggcgta agcgcgggcg ctgatgcgca
     5761 ccggcgagcc gaagcggaga tctcccaggt cggcttgggt cagcccgttg atctgcccta
     5821 cctctcgccc atgcacactg atcattaatt caccgtcgac aatcgactct agcagcacc
//
LOCUS       DAOMFA010000101         5841 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_1758_length_5841_cov_4.935534, whole genome shotgun sequence.
ACCESSION   DAOMFA010000101 DAOMFA010000000
VERSION     DAOMFA010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 5841)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5841)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5841
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1758_length_5841_cov_4.935534"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            112..>306
                     /locus_tag="PKW44_06110"
     CDS             112..>306
                     /locus_tag="PKW44_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR51193.1"
                     /translation="MIQDILLRHADSRNAPAALLAELVAALRPENPARVEQAANLVLS
                     LCHVLDTQPQARAVLRPALVS"
     assembly_gap    307..316
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <317..2182
                     /locus_tag="PKW44_06115"
     CDS             <317..2182
                     /locus_tag="PKW44_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific recombinase"
                     /protein_id="HQR51194.1"
                     /translation="DESISFHDSERAVLRQALLSLLAGRKPVSLYVDSGIQPNTGFFS
                     ELWRRVNRKLLPDDVNPAYLKDLLALIFPRASDEQWVLAVPEETWLELLEAMRFDEEQ
                     AREGWSPVVGGIADALLVLSYRISATGLEPELVRGHPELEDYASPFIAQNIETAKYLV
                     QFAEGEAVAEQDERQVLVMLDQCRQVIERTKRTTSQNGTSIRLTFLLQRLLQQIERQE
                     ALLAMLGALRTDPSGRGAYPLVIALFKALVAAECHKNDVGQHWRENMELLSLRVTENA
                     SRTGEHYITETRREYIGLMRSAMGAGCIIAVMAMFKLIAAGRHMAPLTEAIVFSLNYG
                     LGFVLIELLHFTVATKQPAMTAAAIAASIGESGGKTREMDGLITLIARTMRSQIVAIF
                     GNVMLAVPVAMLIAWSFFLIGGSHFVSPEKALHLLQDTDPVHSGALFYAAVAGVCLFL
                     SGLIAGYHDNLAVYNHIPQRLRGLSWLARLIGQRRLDRMAYYVENNLGALAGNFYFGL
                     LLGGMTGLGVLLGIPLDIRHIAFSSAYFGFSMVGLGFHAPAQATLLAALGVGLIGLTN
                     LLVSFGLALYVAMNARRVTFVQWQALVARLWQRFYRQPREFLLPPPRETSADISQ"
     gene            complement(2203..2718)
                     /gene="yjgA"
                     /locus_tag="PKW44_06120"
     CDS             complement(2203..2718)
                     /gene="yjgA"
                     /locus_tag="PKW44_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HQR51195.1"
                     /translation="MNPSDEITENEPLSKTKRKAAMLALQELGEELIALSSARLAQLD
                     LPEVLMDAVKETQRTTAHGALARQKQYIGRLMREIDTTAIADQLQRWKGTHQEENAHF
                     HQLEKLRTRLLEDDSALADYLHRHPQTDSQQLRTLIRNARREAAAGKPPKSSRELFKL
                     LRATAGEAQNR"
     gene            2799..4094
                     /gene="pmbA"
                     /locus_tag="PKW44_06125"
     CDS             2799..4094
                     /gene="pmbA"
                     /locus_tag="PKW44_06125"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HQR51196.1"
                     /translation="MSEDVLRHAKLSGASAAEAEVSLGIGQNVSVRLEEVETIEYNRD
                     KGMSVTVYFGQRRGHASTSDLSPQALKDTVAAACSIARYTAHDEFCGLADPGLLAREI
                     PELDLYHPWALSVEQAIELAKTCEAAALQVDARINNSEGAGVSTYEGLFAYANSHGFS
                     GGYPTSRHSLSCSVIAEDSDNMQRDYWYSSARAAEDLESAESVGRVAGQRTVRRLGGR
                     QLKTMQVPVLFEANIAAGLLSHFVSAISGGSLYRKSSFLQDSLEQPIFAPHIHISEQP
                     HLKKGLASSPFDNEGVATQARDLVSGGVLHGYMLSSYSARKLGMTTTGNAGGNHNLIL
                     LSGELDFAGLLESMHTGLLVTELLGHGINMVTGDYSRGAAGFWVENGEIQYPVEEITI
                     AGNLKDMFRQIVAVGSDVMVQGSKQTGSILVERMTIAGE"
     gene            complement(4160..4855)
                     /locus_tag="PKW44_06130"
     CDS             complement(4160..4855)
                     /locus_tag="PKW44_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019897455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HQR51197.1"
                     /translation="MGAIMATMVSGCATKGESVEGDPVQGFNRAMYTFNDKLDKYALK
                     PVAQGYKFVLPNFVQTGIYNFFNNLGTLTTIANDLLQFKFSKAMDDSGRFVINTTFGI
                     GGLFDWASKDGINYQKEDFGVTLGTWGWKNSSYLVLPFYGPSTVRDTGGLAVDTLYLD
                     PIYYIDYIPTRNQLLLTKIISTRASYLPASDLLDEAALDPYAFMRDAYLQRRTNLIYD
                     GNPPKENLDDSYN"
     gene            5069..>5841
                     /locus_tag="PKW44_06135"
     CDS             5069..>5841
                     /locus_tag="PKW44_06135"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HQR51198.1"
                     /translation="MHNLTITQFLIEEQRKVGSATGEFTLLLNDIVTACKKISSAVNK
                     GALVGTMGSLESENIQGETQKQLDVITNDIFLETNQWAGYIAGMASEEMDDPYDIPDK
                     YRQGGKYLLLFDPLDGSSNVNVNISVGTIFSILRCPAGVANPKVEDFLQPGTQQVCAG
                     YALYGSSTMLVLTTGNGVNGFTLDRELGEFFLTNPNMRIPADTCEFAVNMSNQRLWEA
                     PVQRYVNECLQGVEGPRAKDFNMRWVASMVAEVTRILIRG"
BASE COUNT         1103 a         1752 c         1763 g         1213 t
ORIGIN      
        1 gttgttttcg agcgtggcgg gcaggcgttt gtaaaaccct tgaagctggg aagccgtcag
       61 tagtttttgc gttacaatcg attccggaat gttcatcctc ccggatccca aatgattcaa
      121 gatattctgt tgcgccatgc cgatagtcgc aatgccccag cggcattgct ggccgagctg
      181 gttgccgcgc tgcgccccga gaatccggca cgtgtggaac aggcggccaa tctggtgctg
      241 tccctgtgcc atgtgctcga tacccagccg caagcccgcg ccgtgctgcg gccggcgctt
      301 gtgtccnnnn nnnnnngacg aaagcatttc cttccacgac tcggaaagag ccgtgctgcg
      361 gcaggcgctg ttgtccctgc tcgcaggccg caagccggtc tccctctatg tcgattccgg
      421 catccagccc aacaccggct tcttttcgga actgtggcgc cgcgtcaacc gcaagctgct
      481 accggacgat gtcaacccgg cctacctgaa agacctgctc gccctgatct ttccccgcgc
      541 ttccgatgaa cagtgggtgc tcgccgtgcc cgaggaaacc tggctggagc tgctggaagc
      601 gatgcgcttc gacgaggagc aggcgcggga aggctggtct ccggtcgtcg gcggaatcgc
      661 cgatgccctc ctggtgctgt cctaccgtat ctcggcgacc gggctggagc cggaactggt
      721 gcgtggccac ccggaactgg aggactacgc gtcgccattc atcgcgcaga acatcgaaac
      781 cgcaaaatac ctggtgcagt ttgcagaagg ggaggctgtc gctgaacagg acgagcgcca
      841 ggtgctggtg atgctagacc agtgccgcca ggtgatcgaa cgcaccaagc gcactacttc
      901 gcagaacggc accagcatcc gactcacctt cctgctgcag cgcctgctgc agcagatcga
      961 gcgccaggag gcgctgctcg ccatgctcgg cgcactgcgt accgatccca gcgggcgtgg
     1021 cgcctaccct ctcgtcatcg cgctgttcaa ggcgctggtg gcggccgagt gccacaagaa
     1081 cgacgtaggg cagcactggc gcgaaaacat ggagttgctg tccctgcgcg tgaccgaaaa
     1141 cgccagccgt accggcgagc attacattac cgaaacgcgc cgcgagtaca tcggcctgat
     1201 gcgctccgcc atgggcgcgg gctgcatcat cgccgtgatg gctatgttca agctcatcgc
     1261 cgccggcagg catatggcac cgttgaccga ggccatcgtg ttcagcctga attacggctt
     1321 gggcttcgtg ctgattgaac tgttgcattt caccgtggcc accaagcagc cggccatgac
     1381 tgccgctgcc atcgccgcca gcatcggcga aagcggcggc aagacgcgcg aaatggacgg
     1441 gctgatcacg ttgatcgccc gcaccatgcg cagccagatc gtcgccatat tcggcaacgt
     1501 gatgctggca gtgccggtgg cgatgctgat cgcctggagt tttttcctga tcggcggcag
     1561 ccatttcgtt tcaccagaga aagccctgca tctgctccaa gacaccgacc cggtgcacag
     1621 tggcgcgctg ttctatgccg ccgtcgccgg cgtctgcctg ttcctctccg ggctgatcgc
     1681 cgggtatcac gacaacctgg cggtttacaa ccatatcccg cagcgcctgc gcgggctgtc
     1741 ctggttggcc aggctgatcg gacagcgccg cctggaccgt atggcgtatt acgtggaaaa
     1801 caatcttggc gcactggccg gaaatttcta tttcggcttg ctgcttggcg gcatgacggg
     1861 gctgggcgtg ctgctcggga ttccgctgga tatccggcac atcgcgttct cctctgccta
     1921 tttcggcttt tccatggtcg ggctggggtt ccacgcgccc gcgcaggcca cgctgctggc
     1981 cgcgctcggg gttgggctga tcgggctaac caacctgctg gtcagtttcg gcctggcgct
     2041 ctacgtggcg atgaatgcgc gtcgggtgac tttcgtgcag tggcaggcac tggtcgccag
     2101 gctatggcag cggttttacc ggcagccgcg cgaattcctc ctgcccccgc cgcgggagac
     2161 aagcgcggac atcagccagt aacgctgacg ctacgaactg gcttagcggt tttgcgcttc
     2221 gccggccgtt gcgcgtagca gtttgaacag ttcgcgacta cttttcggcg gcttcccggc
     2281 agcggcttcc ctgcgggcgt tgcggatcag ggtgcgcagt tgctggctgt cggtctgggg
     2341 gtgccggtgc aggtaatccg cgagcgcgct atcatcttcc agcaggcggg tgcgcagttt
     2401 ttccagttgg tggaaatgcg cattttcctc ctgatgcgtg cctttccagc gttgcaactg
     2461 gtcggcgatg gcggtcgtgt cgatttcgcg catcagcctg ccgatgtact gtttctgccg
     2521 cgccagcgcg ccgtgcgccg tggtgcgttg ggtttccttg acggcatcca tcagtacttc
     2581 cggcaagtca agctgcgcca gccgggcgct actgagggcg atcagctctt cgccaagttc
     2641 ctgcaaggcc agcatggcgg ccttgcgttt ggtcttgctg aggggttcgt tttcggtgat
     2701 ttcgtctgac ggattcatgc tgcggtatga taacagcttt ttgaaagccc cccgtgagcg
     2761 aaccccgttt cagctattct cccgacgacc tgcgccagat gagcgaggac gtgctgcgtc
     2821 acgccaaatt gtcaggcgcc agcgccgccg aggcggaagt cagcctcggc atcggccaga
     2881 acgtgtcagt acggctggag gaagtggaga ccatcgagta caaccgcgac aagggcatgt
     2941 cggtcacggt gtatttcggc cagcggcgcg gccatgccag tacctccgac ctctcgccgc
     3001 aggcgctgaa ggacaccgtg gcagccgctt gcagcatcgc ccgctatacc gcgcatgacg
     3061 aattctgcgg cctggccgat ccgggactgc tggcaaggga gattcccgag ctcgacctgt
     3121 accacccgtg ggcgctgtcg gtggagcagg caatcgagct ggccaagacc tgcgaggctg
     3181 cagcgctgca ggtggacgca cggatcaaca actcggaggg cgccggcgtt tccacttatg
     3241 aaggcctgtt cgcctacgcc aacagccacg gcttcagcgg cggctacccg acttctcgcc
     3301 attcgttgag ctgttccgtc atcgccgagg atagcgacaa catgcagcgc gactactggt
     3361 acagctcggc acgtgccgcc gaagatctgg aaagcgccga gtccgtcggc cgcgtggctg
     3421 gccagcgcac cgtgcgccgt ctgggtgggc gccagctcaa gaccatgcag gtaccggtgc
     3481 tgttcgaggc caatatcgcc gctggcctgc tgtcgcattt cgtctcggcg atctccggcg
     3541 gcagcctgta ccgcaaatcc tcgttcctgc aggacagcct ggaacagccg atatttgcgc
     3601 cgcatatcca tatcagcgag cagccgcacc tgaaaaaagg cttggccagt tcgccgttcg
     3661 acaacgaggg cgtggcgacc caggcgcgtg atctggtcag tggcggcgtg ctgcacggct
     3721 acatgctgtc cagctactcg gcgcgcaaac tcggcatgac gactaccggc aacgctggcg
     3781 gcaaccacaa cctcatcctg cttagcggcg aactggattt cgccggattg cttgaatcca
     3841 tgcataccgg cctgttggtg acagaacttc tggggcatgg catcaacatg gtcaccggcg
     3901 actactcgcg aggcgcggcc ggcttctggg tggaaaacgg cgagattcag tacccggtgg
     3961 aggagatcac cattgccggc aatctcaagg acatgttccg tcagatcgtc gcggtcggca
     4021 gtgacgtcat ggtgcagggc tccaagcaga ccggctccat cctggtggag cgtatgacga
     4081 tcgccggcga gtgattgcac cctacaagta aaaagccccg catcgcgggg ctttttactt
     4141 gaggattttc ctggatgact tagttgtagg agtcgtccag gttttccttc ggcggattgc
     4201 cgtcatatat caaattggtg cggcgctgca agtaagcgtc acgcatgaac gcatacggat
     4261 ccagcgccgc ttcgtccaga agatcgctgg ccggcaggta ggaggcacgg gtgctgatga
     4321 tcttggtcaa tagcagctgg ttgcgggtgg ggatatagtc gatataatag atcgggtcga
     4381 ggtacagcgt atccactgcc aagccaccgg tatcgcgcac cgtgcttggc ccatagaaag
     4441 gcagcaccag atagctgctg ttcttccagc cccaagtgcc gagcgttacg ccgaaatctt
     4501 ccttctggta gttgatgccg tccttggatg cccagtcgaa caggccgccg atcccgaagg
     4561 tggtattgat gacgaagcga ccgctgtcat ccatcgcctt gctgaatttg aactgcaaca
     4621 aatcgttggc gatcgtggtg agggtgccga ggttgttgaa gaaattatag ataccggttt
     4681 ggacaaaatt cggcaataca aacttatagc cttgggccac cggtttcagg gcgtacttgt
     4741 caagcttgtc gttgaacgtg tacatggcgc ggttaaaacc ctgcaccgga tctccttcaa
     4801 cgctctcgcc cttggtggcg caaccgctca ccatggtggc catgatggca cccacgacta
     4861 aacccctgaa atccatctta tgcataggaa accttgttat tttgtgttga cgaaggcgga
     4921 ataagccttc cacgatgaat ggcgcaactt taacggacga ccatggccct gtctagcgct
     4981 tttgcacgtt atactgtggt tgtgttgtaa aaatgttaca acccatttca gggacttttc
     5041 ttaatccagg atttagcgag aatagagcat gcataacttg accattaccc agttcctgat
     5101 tgaggaacaa cgcaaagtag gcagtgccac cggtgaattt accttgctat tgaacgacat
     5161 cgtgaccgcc tgcaaaaaaa tctcctctgc cgtgaacaag ggggcgctgg taggcaccat
     5221 gggcagcctg gagtcggaaa acatccaggg tgaaacacag aagcaactgg acgtcatcac
     5281 caatgatatt ttcctggaaa ccaaccagtg ggcgggctac atcgccggca tggcgtcgga
     5341 ggagatggac gacccctacg acatcccgga caaataccgc cagggcggca agtacctgct
     5401 gctgttcgat ccgctcgacg gctcgtccaa cgtcaacgtc aacatctcag ttggcaccat
     5461 tttttccatc ctgcgctgcc ctgccggggt agccaacccc aaggtggaag atttcctgca
     5521 gccaggcacc cagcaggtct gcgccggcta tgcgctgtac ggctcctcca ccatgctggt
     5581 gctgaccact ggcaacggcg tcaacggctt cacgctggat cgcgaactgg gcgagttctt
     5641 cctcaccaat cccaacatgc gcattcctgc cgacacctgc gaattcgccg tcaacatgtc
     5701 caaccagcgc ttgtgggagg cgccggtaca gcgctatgtg aacgagtgct tgcaaggcgt
     5761 ggaaggccca agggctaagg atttcaacat gcgctgggtg gcctctatgg tggcggaagt
     5821 gacccgcatc ctgatccgcg g
//
LOCUS       DAOMFA010000102         5828 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_1765_length_5828_cov_5.536290, whole genome shotgun sequence.
ACCESSION   DAOMFA010000102 DAOMFA010000000
VERSION     DAOMFA010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 5828)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5828)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5828
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1765_length_5828_cov_5.536290"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..905)
                     /locus_tag="PKW44_06140"
                     /pseudo
     CDS             complement(1..905)
                     /locus_tag="PKW44_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020165288.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
     gene            complement(1016..2857)
                     /locus_tag="PKW44_06145"
     CDS             complement(1016..2857)
                     /locus_tag="PKW44_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019918993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQR51199.1"
                     /translation="MYRWFEKLVNPFPETVVAPPSKGFWAFAWSCTEGMRGYMLAMTL
                     LTASIGAFEALLFGILGRVVDWLSHVTPALLWTQERGKLLLLVAILLGSIVLVVLQTI
                     IKHQTLAGNFPMRLRWNFHRLMLNQSMTFYQDEFAGRIASKVMQTALAVRDMWFILTD
                     ILVFVVIYFVTLLAIVDSFDPWLMLPFFGWLVLYVGMQWVFVPRLGKVSQAQADARAS
                     MTGRIADAYTNIATVKLFSHAGREAGYAKSAMQEFIGTVHRQMRLVSSVEILNHVLIM
                     LLIIASAGIALRLWSLGTVGVGAVAAVTAMVLRLHGMSHWIMWEMTSLFEHVGTVQDG
                     INTLSTVQLVTDRAAARPLVVRQGDIRFEAVNFAYGGQQQVLDNFALHIWPGEKVGLV
                     GRSGAGKSTIVNLLLRFYDVEAGHIFIDGQDIADVMQNSLRENIGMVTQDTSLLHRSV
                     RENILYGRPDAIEEDMVSAARRAEAHDFILGLRDPKGRNGYDAHVGERGVKLSGGQRQ
                     RIAIARVMLKDAPILLLDEATSALDSEVEAAIQGSLYRLMEGKTVVAIAHRLSTIAAM
                     DRLVVLDQGRIIEEGDHETLLERGGLYAKLWVHQSGGFLGDDDKELN"
     gene            complement(3039..3365)
                     /locus_tag="PKW44_06150"
     CDS             complement(3039..3365)
                     /locus_tag="PKW44_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HQR51200.1"
                     /translation="MSDIIIEHKPNKTRLDALDVFQWPTWSKEVSVFPWVFHEQEIAW
                     ILEGECVITPENGAPVKFGKGDLVTFPAGLKCNWEVRQPLHKHYHLDGSKLGQIARRV
                     RAMLPF"
     gene            3509..4732
                     /locus_tag="PKW44_06155"
     CDS             3509..4732
                     /locus_tag="PKW44_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQR51201.1"
                     /translation="MTILLPAYLNPAFLRLLTFRVLIVLAYQITAVVVGWHIYELTHS
                     TLALGLVGLAEVIPYFSFALFAGYAVDHYSRRLFGVLAAVMLFFNALTLTAVAAGWLV
                     GDAALWIYASIAFTGVARAFIGPSYNSMFALVLPREHYARAAGIGSSAFQFGLVAGPA
                     LGGVLVGWASTTMAYAVAAILCLGAAMALLSLRVNEPRPAQSAPVFTSIAEGLRFVRG
                     NQIVLGAMSMDMIAVLFGGAVAMLPAFVQDVFHAGPEGLGILRAAPAVGAVVTGLVLA
                     RHPLSRHAGRWLLGAVGGFGLCIIGFALSPHFWIAGMLLLLSGAFDGVSVVMRTTILQ
                     LATPDAMRGRVSAINGIFIGSSNELGAFESGLAARLMGLVPSVIFGGTMTLVVVVATA
                     RLAPKLRRLDLRELH"
     gene            4822..5031
                     /locus_tag="PKW44_06160"
     CDS             4822..5031
                     /locus_tag="PKW44_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012777486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HQR51202.1"
                     /translation="MSAHIYKMIELVGSSTIGTDDAIRNAIAKAAQSVRHMDWFEVVE
                     TRGHIADGKVAHYQVTLKVGFRLED"
     gene            complement(5052..5297)
                     /locus_tag="PKW44_06165"
     CDS             complement(5052..5297)
                     /locus_tag="PKW44_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR51203.1"
                     /translation="MRLNDQLKSAITAALAGEWDKSHRIVQEFSDPTACRIHAVLHKI
                     EGDAWNSRYWYARTHGAQYEDFADPKAELEAILQELQ"
     gene            5337..5696
                     /locus_tag="PKW44_06170"
     CDS             5337..5696
                     /locus_tag="PKW44_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HQR51204.1"
                     /translation="MRNLDDLFAALAKSAFRRSFHLRGKDLDYLRSKGLPVVLTHAED
                     FVSKRLAPGFISNDGKQTPFRGHPVFVAQHATACCCRGCLEKWHRIPPERELTQEETA
                     YILMVLERWLREESAGE"
BASE COUNT         1018 a         1735 c         1834 g         1241 t
ORIGIN      
        1 gcgttatcgc agatgcggtc catgcgtaaa ggaagggttt caatgccttg aagtatgaga
       61 aatgcgttga aaggggagat tgccgcgccc atgttgcgga gcggaactac ccgagctctt
      121 gctatgtagg ctgcgggccc cattgtttca gtgtagacca cgccatggta gctaacgtct
      181 ggctagatca ggcgcttgaa tcgcgctttg tgttcagccc aggggaattt cccggaatcc
      241 acgatagctc cgccaaggct attgccatgg ccgccaaggt acttggtcag cgaatgtact
      301 acgatatcag caccatgctc aaacgggcgg cacaggtaag gactgggcac ggtgttgtct
      361 acaataagcg gtacgccgtg acggtgtgca atttcggcaa gcgcggtaat gtctgtgatg
      421 ttgccgcgag gattgcccaa cgattcggcg aagatggcct tggtcttgcc gtctatcagg
      481 gcttcgaaac tttttgggac atccgaattg gcaaagcgtg tttcgatccc gtactgaggt
      541 agcgtgtgtg caaacaggtt gtaggtgccg ccgtacaagg ccgtggaaga aacgatgttg
      601 tcgcctgctt ccgcaatggt ctggatggcg taggtgatgg ccgcctgtcc cgaggccagc
      661 gcgagcgcgg caatgccgcc ttccagggct gccactcgtt cctccagcac ggattgtgtc
      721 gggttcatga tgcgggtgta aatgttgccg ggcactttga ggtcgaacag gtcagccccg
      781 tgctgggcat tgtcaaaggc atatgcgact gtctggtaaa ttgggggcac caccgctttg
      841 gtcgttggat ctgggctgta gccggcatgg acagaacggg tttcaaactt ccagttttca
      901 gacatttgga tggctccttt ggctatggtg gatgttgcgg gctagctgcg taaattctag
      961 caaagctagc aggctgagtg tcactggaac aagcgctact acaccagcat ttcctctaat
     1021 ttagctcctt gtcatcgtcc cccaagaatc ctccgctctg gtgcacccac agcttcgcat
     1081 agaggccacc gcgttcgagc agggtttcgt ggtcgccttc ctcgatgatg cgaccctgat
     1141 ccagcacgac cagacggtcc atggcggcga tggtggagag acggtgcgcg atggcaacca
     1201 cggtcttgcc ttccatcagg cggtacaaac tgccttgtat cgcggcttcg acctcggagt
     1261 cgagcgcgct ggtggcctcg tccagcagca ggatgggcgc atccttcagc atcacccgcg
     1321 caatggcgat gcgttggcgc tggccgccgg aaagcttgac gccgcgctcg ccgacgtggg
     1381 cgtcgtagcc gttgcggccc ttggggtcgc gcaggccgag gatgaagtcg tgcgcctcgg
     1441 cgcgccgggc ggccgaaacc atgtcttcct cgatggcgtc ggggcggccg taaaggatgt
     1501 tctcgcgcac cgagcggtgc agtagcgagg tgtcctgcgt caccatgccg atgttttcgc
     1561 gcaggctgtt ctgcatgacg tcagcgatgt cctgcccgtc gatgaaaata tggccggcct
     1621 ccacgtcgta aaagcgcagc agcaggttga cgatagtgga tttgccggca ccggagcggc
     1681 cgaccaggcc gaccttctcg cccggccaga tgtgcagcgc gaagttgtcc aacacctgtt
     1741 gctggccgcc gtaggcgaaa ttgactgcct cgaagcggat gtcgccctgc cgcaccacca
     1801 gcggtctggc agcggcgcgg tcggtcacca attgcacggt ggagagcgtg ttgatgccgt
     1861 cctgcacggt gccgacgtgc tcgaacagcg aggtcatttc ccacatgatc cagtgcgaca
     1921 tgccgtgcag gcgcagcacc atggcggtca ccgccgccac cgcgcccacg cctacggtgc
     1981 cgaggctcca cagccgcaac gcgatgccgg cgctggcgat gatgagcagc ataatgagca
     2041 cgtggttgag gatttccacg ctgctcacca gccgcatctg ccggtgtacg gtgccgatga
     2101 actcctgcat cgcggatttt gcatagccgg cttcgcgccc ggcatgggag aacagcttga
     2161 cggtggcgat gttggtatag gcatcggcga tgcgcccggt catgctggca cgtgcgtctg
     2221 cctgcgcctg cgacaccttg ccgaggcgcg gcacgaaaac ccactgcatg cctacgtaca
     2281 gcaccagcca gccgaagaag ggcagcatca gccaggggtc aaaactgtct acgatggcga
     2341 gcagggtgac gaagtagatc accacgaata ccaggatgtc ggtgaggatg aaccacatgt
     2401 cgcgcaccgc cagcgcagtc tgcatcactt tcgatgcgat gcggccggcg aactcgtcct
     2461 ggtagaacgt catgctctgg ttcagcatga gccggtggaa attccagcgc agccgcatgg
     2521 gaaagttgcc ggccagcgtc tgatgcttga tgatggtttg cagtacgacc agtacgatgc
     2581 tgccgagcag gatggccacc agcaacagca gcttgccgcg ctcctgcgtc catagcaagg
     2641 ccggcgtcac gtggctcagc cagtccacta cgcggcccag gatgccgaac agcagcgcct
     2701 cgaaggcgcc gatagacgcg gtcagcagcg tcatcgccag catgtagccg cgcatgccct
     2761 cggtgcacga ccaggcaaac gcccagaagc ctttcgacgg cggtgcgacc acagtctccg
     2821 ggaacgggtt gaccagcttt tcgaaccagc ggtacatcgg ttttccgggt tgaatcagaa
     2881 agggttagtg cgcctaatct atatcggcag actcacacta ggtcaatgag agctcatcgg
     2941 tccggcgcac cggacttact gccttattca ctgcactaac ccccgatatt ctacccgtca
     3001 accgggccga ataaggcttt cttgccggga aggctgcttc agaacggcag catcgccctg
     3061 actcggcggg caatttgccc cagtttgctg ccgtccaggt ggtagtgctt gtgcagcggc
     3121 tggcgcactt cccagttgca tttgaggccg gcgggaaagg ttaccaggtc gcccttgccg
     3181 aatttcaccg gcgcgccgtt ttcgggcgtg atcacgcatt cgccttccag tatccaggcg
     3241 atttcctgct catggaaaac ccacgggaat acggaaactt ccttcgacca ggtcggccac
     3301 tggaaaacgt ccagtgcatc caggcgggtt ttgttcggtt tgtgttcgat gatgatgtcg
     3361 ctcatggtgt tgtcctcgga tggtcattgg gatggggcgc gatgttacca gcacgggcgg
     3421 gtttgtccta acagcgtggc gtgaagggat gcgataatca ctgcttttcg gctttgatgc
     3481 ggcctgatcc cgcaatctga cattgctcat gacaatcctg cttcccgctt acctcaaccc
     3541 cgcattcctg cgactgctga ccttccgcgt cctgatcgtg ctggcctacc agatcacggc
     3601 agtggtggtc ggctggcaca tttacgagct gacgcacagc acgttggcgc taggcctggt
     3661 cgggctggcc gaggtgattc cctatttcag ctttgccctg tttgccggct atgcggtgga
     3721 tcattattcg cgtcgcttgt tcggtgtgct ggcggcggtg atgctgtttt tcaacgcgct
     3781 gacgctgacg gcggttgccg ctggctggct ggttggtgat gccgcgctgt ggatttatgc
     3841 gtccattgcc ttcaccggcg tggcgcgcgc cttcatcggc ccatcctata actcgatgtt
     3901 cgcgctggtc ctgccgcgcg aacattacgc gcgcgccgcc ggcatcggca gttccgcttt
     3961 ccagttcggg ctggtggccg gccccgcgct ggggggcgtt ctggtcggct gggccagcac
     4021 cacgatggcc tacgcggtgg cggctatcct gtgcctgggt gccgccatgg cgctgctgtc
     4081 gctgcgggtg aatgagccgc ggccggcgca aagtgcaccg gtgttcacca gcattgccga
     4141 gggcctgcgc ttcgttcgcg gcaaccagat cgtgctcggc gccatgtcga tggatatgat
     4201 cgccgtgctg ttcggcggcg cggttgccat gctgcccgcg tttgtccagg acgtgttcca
     4261 tgctggcccg gaagggctgg gcatcctgcg cgcagcgccg gcggtcggcg ccgtggttac
     4321 cgggctggtg ctggcgcgcc atcccctcag ccgccacgca gggcgctggc tgctgggcgc
     4381 ggtggggggg tttggcttgt gcatcatcgg tttcgccctc agcccgcatt tctggattgc
     4441 cggcatgctg ttgctgctgt ccggtgcctt cgacggggtc tcggtggtga tgcgcaccac
     4501 catcctgcag ttggccacgc cggacgccat gcgcggccgg gtttcggcca tcaacggcat
     4561 tttcatcggc tcctccaacg aactcggcgc ctttgaatcc ggcctggcgg cgcggctgat
     4621 ggggctggtg ccttcggtca tcttcggcgg cacgatgacg ctggtggtgg tggtggcgac
     4681 tgcccggctg gcgcccaagc tgcgtcggct ggacttgcgg gagttgcatt aaacctgttg
     4741 ccctgttgta ggagcggcgc ctcgccgcga taatgatatc gcgctgggga gaacgcccca
     4801 gcctgaataa aggagaacga tatgtcagca cacatctaca aaatgatcga actggtgggc
     4861 tcctcaacca tcggtaccga cgacgccatc cgcaacgcca tcgccaaggc ggcgcaaagc
     4921 gtccgccaca tggactggtt cgaggtggtg gaaacccgcg ggcatatcgc ggatggcaag
     4981 gtcgcccatt accaggtcac gctcaaggtc ggcttccggc tggaggactg accgggcggg
     5041 aggtcatgcg cctactgcaa ctcctgcagg atcgcctcca gttcggcttt ggggtcagcg
     5101 aaatcttcat actgtgcgcc gtgcgtccgc gcataccagt agcggctgtt ccatgcgtct
     5161 ccctcgattt tgtgcaggac ggcgtggatg cgacacgccg tcgggtcgct gaactcctgc
     5221 acgatacggt gcgatttgtc ccattcgccc gccagtgcgg cggtgatggc tgatttcagt
     5281 tgatcgttca gacgcatttc ggcttctccg taacttgcca tgctctagtt taacgtatga
     5341 gaaacctcga cgatcttttc gccgcgctgg ccaaatccgc tttccgccgc agctttcatc
     5401 tgcgtggtaa ggatctcgac tatctgcgca gcaaaggcct gcccgtggtg ctcacccacg
     5461 ccgaggattt cgtctccaaa cgcctcgctc cgggctttat ttccaacgac ggcaagcaga
     5521 cgccgtttcg gggccatccg gtattcgtcg ctcagcacgc cactgcgtgc tgctgccgcg
     5581 gctgcctgga aaaatggcat cgcattccgc ccgaacgtga actcacgcag gaagaaacgg
     5641 cctatatttt gatggtgctg gaacgctggc tgcgggagga gtcggctgga gaataatgcc
     5701 tgctacaacc agtcccagtc cagccgcggc ttgtgcggcg ctttcctcat cgcccagtcc
     5761 cacagcgggg cggggatcag cttcatcagc cgccccagta gggccatctg ccaaggcagg
     5821 atgacgta
//
LOCUS       DAOMFA010000103         5750 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_1793_length_5750_cov_4.809658, whole genome shotgun sequence.
ACCESSION   DAOMFA010000103 DAOMFA010000000
VERSION     DAOMFA010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 5750)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5750)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5750
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1793_length_5750_cov_4.809658"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            148..1059
                     /gene="gnd"
                     /locus_tag="PKW44_06175"
     CDS             148..1059
                     /gene="gnd"
                     /locus_tag="PKW44_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HQR51205.1"
                     /translation="MKIGMVGLGKMGANMVRRLRRGGHEVVGYSLETGAVQQLADECG
                     MEKAGSLADVVRQLPAPRVLWLMLPAGEVTEDTLNQLKGLLSAGDVVIEGGNSYYKDS
                     QRRAEELKKIGVGYVDAGTSGGIWGLENGYALMVGGERDVVAKVEPVIKTLAPAPDKG
                     WLHCGPSGSGHFTKMIHNGIEYGMMQAYVDGFALMKGKAEFDLDLAAIAEMWRHGSVV
                     RSWLLDLTADFLAKDQELESIEPFVADSGEGRWTVLESVEQGVPTPVMTMALMRRFAS
                     QGKDIFPNKMLAMMRKGFGGHAVKTGE"
     gene            1065..2555
                     /gene="zwf"
                     /locus_tag="PKW44_06180"
     CDS             1065..2555
                     /gene="zwf"
                     /locus_tag="PKW44_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018076260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HQR51206.1"
                     /translation="MSSQETIPGPCHFVIFGATGNLATIKLLPCLYNLEVAGRIPGDM
                     SILALGRQEHTQESWLAHLSKVLHDKFGDKVDPAVAERFAKRFSYMFGDLREAAFYQR
                     LKDKLAEPREGSCSNIVFYLSIIPKDFNTVINFLDEFGFSQPRGLHRIVVEKPFGTDL
                     ESAQLLNRSLHKHFDEEQIYRIDHYLGKETVQNLLVYRFANTLIEPLWNRNYINHVQI
                     TVAEDFGIGTRADYYDKSGGAMRDMLQNHLMQLLTVVAMEPPATMEADAVRDEKVKVL
                     KSMRIIPEDKVNEHVVRAQYAAGTVRGQPVHGYMQESGVDPESSTETFTAAKFYIDNW
                     RWRGVPFYLRTGKRMAKTQSTIAIRFRHPPQHLFHDESFEHIEPNWIYLSLQPTEHMH
                     MEMHVRAPGLGMDTRVVSLNASYRQEEDEKPLEAYQALLLDVIRGDKTLFIRFDEVEW
                     AWRVVEPILKVWGAGNVPVHSYPAGTWGPEETSSLFDCHDQAWRND"
     gene            2563..2946
                     /gene="crcB"
                     /locus_tag="PKW44_06185"
     CDS             2563..2946
                     /gene="crcB"
                     /locus_tag="PKW44_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HQR51207.1"
                     /translation="MGGGGFIAVGVGAALGAWLRWGLGLLLNPVLPTLPLGTLAANLV
                     GGYLMGIAMGIISHYASLPAEARLLITTGFLGGLTTFSTFSAEAVALLSRQQYGWAAA
                     HISSHLLGSLAMTVLGVITINLLKG"
     gene            2949..3266
                     /locus_tag="PKW44_06190"
     CDS             2949..3266
                     /locus_tag="PKW44_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018077453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF190 domain-containing protein"
                     /protein_id="HQR51208.1"
                     /translation="MQGVYLKFYVAESSKHHGKLLYEWLLEEARRIGIPGGSASRAIA
                     GYGRHGRMHEEHFFELAGELPVMIEFVAEEEAISGLLERLQQEKIAIFYIKLPVQGGI
                     TTT"
     gene            3397..>5750
                     /gene="gyrA"
                     /locus_tag="PKW44_06195"
     CDS             3397..>5750
                     /gene="gyrA"
                     /locus_tag="PKW44_06195"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019898767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HQR51209.1"
                     /translation="MDQFAKETLPVSLEEEMRRSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMHELSNDWNRPYKKSARIVGDVIGKYHPHGDTAVYDTIVRMAQDFSLRYML
                     VDGQGNFGSVDGDNAAAMRYTEIRMARIAHELLGDLDKETVDFGPNYDGSEHEPLVLL
                     AKIPNLLMRGASGIAVGMATNIPPHNLNEVIDACLAVLQNVDITIDELIDIVPAPDFP
                     TAGIIYGTVGVKEGYRTGRGRVVMRGRTHFEDIGKGDRQAIIVDELPYQVNKKTLIEK
                     IAELVNEKKIDGISDLRDESDKSGMRMVIELKRGEVPEVVLNNLYKQTQLQDTFGMNM
                     VALVDGQPRLLNLKQMLEAFLRHRREVVTRRTVFELRKARNRGHVLEGLAVALANVDE
                     MIALIKAAPTPPDAKVALMARSWKSPVVEEMLKRAAADASRPEGLDPAYGLQANGYKL
                     SDVQAQEILQMRLQRLTGLEQDKIVSEYKEVIDVITDLLDILAKPERVTEIIAGELAA
                     IRQQFGDKRRSEIEHNAQDISLEDLITPQDVVVTLSHTGYVKSQPLDEYRAQRRGGRG
                     KQAAATKEDDFIDKMFVANTHDYILCFSSRGRVYWLKVYEVPQGSRISRGKPIVNLFP
                     LEEGEKINAILSVKEFDENHFIFMATAQGTVKKTALVEFSNPRKAGIIAINLDDGDYL
                     IGAEVTNGANDVVLVSDGGKAVWFDEEDVRHMGRATRGVRGMKLQAKQQVLSLLIAEH
                     DKQTVLVATENGYGKRTVLADFRHSGRGTQGVIAIATSARNGKVV"
BASE COUNT         1244 a         1644 c         1748 g         1114 t
ORIGIN      
        1 tgcaacggat ttttctttct tttgagggcc ccggccaatt ataattattc gattgtgggg
       61 cgcgaattat gctctacagc gttgatgata ctggtgggcg cgagcccgtg caaggctgta
      121 gggccaattt aacgaaaggg gagtctcttg aaaatcggga tggtaggatt gggcaagatg
      181 ggcgctaaca tggtgcgccg attgcgccgg ggcggtcatg aggtagttgg ctatagcttg
      241 gaaacgggtg cggtccagca acttgccgac gaatgtggga tggaaaaagc cgggtcattg
      301 gccgatgtag tcaggcaatt gccggctcca cgcgtcctct ggctgatgct gcctgccggc
      361 gaagtgaccg aagacacgct caaccaattg aaagggctgt tgtccgcagg agacgtcgtg
      421 atcgaggggg gcaactccta ttacaaggat tcgcagcgtc gcgccgagga attgaagaaa
      481 attggcgtcg gttacgtgga tgccggtact tccggcggta tctggggact ggaaaacggc
      541 tacgccttga tggtgggcgg tgaaagggat gtcgtggcca aggtagagcc tgtcatcaag
      601 acgctggcgc cggcgccgga caagggctgg ctgcactgtg gcccaagcgg ttccggccat
      661 ttcaccaaga tgatccataa cggcatcgag tacggcatga tgcaggctta tgtcgatggc
      721 ttcgctctga tgaagggcaa ggccgagttt gatcttgacc ttgccgccat cgctgaaatg
      781 tggcgtcacg gcagcgtggt acgcagctgg ttgctcgatc tgaccgccga cttcctggcc
      841 aaggaccagg agctggaatc catcgagccg ttcgtggccg attccggcga agggcgctgg
      901 acagtgctgg agtcggtaga gcagggcgtg ccgacgccgg tgatgaccat ggcgctgatg
      961 cggcgctttg ccagccaggg caaggacata ttccccaaca agatgctggc catgatgcgc
     1021 aagggctttg gcggccacgc ggtaaaaaca ggagagtaag cgttatgagc agtcaagaaa
     1081 ccatcccggg gccgtgtcat ttcgtcattt ttggtgccac cggcaatctg gccaccatca
     1141 agctgttgcc gtgcctgtac aacctggaag tcgccgggcg catccccggc gacatgagca
     1201 tcctggcgct ggggcgccag gagcacaccc aggaatcctg gctggcacac ctcagcaagg
     1261 tgctgcacga caagttcggc gacaaggtcg atccggccgt ggccgagcgt ttcgccaaac
     1321 gcttctccta catgttcggc gacctgcggg aagcggcgtt ctaccaacgc ctcaaggaca
     1381 agctggcgga gccgcgcgag ggctcctgca gcaacatcgt gttctacctg tccatcatcc
     1441 ccaaggattt caataccgtc atcaatttcc tcgacgagtt cggcttctcc cagccccgcg
     1501 gcctgcaccg catcgtggtg gaaaagccgt tcggcaccga cctggaaagc gcccagttgc
     1561 tcaaccgctc gctgcacaag cacttcgatg aagaacagat ctaccgtatc gaccattacc
     1621 tcggcaagga aacggtgcaa aacctgctgg tgtaccgctt tgccaacacc ctgatcgagc
     1681 cgttgtggaa ccgcaactac atcaaccacg tgcagatcac cgtggcggaa gacttcggca
     1741 tcggcacgcg cgccgactat tacgacaagt cgggcggtgc catgcgcgac atgctgcaga
     1801 accacctgat gcaattgctg acggtcgttg ccatggagcc tcccgccaca atggaggcag
     1861 atgccgtgcg cgacgaaaag gtcaaggtac tcaagtccat gcgcatcatt cccgaggaca
     1921 aggtcaacga gcacgtggta cgtgcgcagt atgccgccgg cacggtcagg gggcagcctg
     1981 ttcatggcta catgcaggag tcgggggttg atcccgaatc gagcactgaa accttcactg
     2041 ccgccaagtt ctacatcgac aactggcgct ggcgcggggt gccgttctat ctgcgcaccg
     2101 gcaagcgcat ggccaagacc caatccacca ttgccatccg cttccgccac ccgccgcagc
     2161 atttgttcca cgacgagtcg ttcgagcata tcgagccgaa ctggatttac ctgtccctgc
     2221 agccgaccga gcacatgcac atggaaatgc acgttcgggc gccggggttg ggcatggata
     2281 cccgtgtggt gtcactcaat gcatcctatc gccaggaaga ggacgaaaag ccgctggaag
     2341 cctaccaggc gctgctgctg gacgtgatcc gcggcgacaa gaccctgttc atccgttttg
     2401 acgaggtcga gtgggcgtgg cgcgtggtgg agcccatcct caaggtttgg ggcgcaggca
     2461 atgtgccggt tcactcttac cctgcaggca cctgggggcc ggaagaaacc agcagcttgt
     2521 tcgactgcca cgaccaagcc tggcgcaatg attagctgaa gcatgggtgg cggtggcttc
     2581 attgcggtag gtgtcggcgc cgccctgggt gcctggttgc gctgggggct ggggttgctg
     2641 ctgaatcccg ttctgcccac ccttccgctg ggtacgctgg cggccaatct ggtcggtggc
     2701 tatctgatgg gaattgccat gggcatcatc agccactacg ccagcttgcc agcagaagcc
     2761 cggctactta tcactaccgg cttccttggc gggctgacga ccttttccac tttttctgcc
     2821 gaggcggtgg cgttgctgtc gcgccagcag tacggctggg cggcggcaca tatttcctca
     2881 cacctgctgg gttcactcgc catgactgtg ctcggcgtta tcaccatcaa tctgcttaag
     2941 ggatgaccat gcaaggtgtt tatctcaaat tttatgttgc tgaatcatcc aagcatcacg
     3001 gcaagctgtt atacgaatgg ctgctggaag aggcacggcg tatcggcatt ccgggcggat
     3061 ccgcgtctcg cgcgattgcc ggctatggac ggcatggccg catgcacgag gagcattttt
     3121 tcgagctggc cggagagttg cccgtgatga tcgaatttgt cgctgaagag gaggcgattt
     3181 cgggattgct ggaacgcctg caacaagaaa aaattgctat tttttacatc aagttacccg
     3241 ttcaaggcgg catcactaca acttgagcgc cagcatgaga aaatccctgc catttcatcc
     3301 ttctccgggg tgctacaggg gctgctcccg tgctaaaatc caagccttaa tttcccggct
     3361 gaaaccgctg ccgttcacta caaaaatagg ccattaatgg atcagttcgc caaagaaacc
     3421 ctccccgtca gtctcgaaga agaaatgcgc cgttcctacc tcgattacgc catgagcgtg
     3481 atcgtggggc gtgcgctgcc agatgtccgc gatggcctga aaccggtgca tcgccgcgtg
     3541 ttgtatgcca tgcacgagct ttccaatgac tggaaccgtc cgtacaagaa atcggcacgt
     3601 atcgtcggcg atgtcatcgg taaataccac ccgcacggcg acacggcggt gtacgacacc
     3661 atcgtgcgca tggcacagga tttctcctta cgctacatgc tggtggacgg gcagggcaac
     3721 ttcggttcgg tggacggcga caacgcggct gccatgcgct acacagaaat ccgcatggcc
     3781 agaattgcac acgaactgct tggcgatctg gataaggaaa cggtcgattt cggacccaac
     3841 tacgacggct ccgagcatga accactggta ttgctggcca agatccccaa cctgctaatg
     3901 agaggtgcgt ctggtattgc ggtgggcatg gcgaccaata ttccgccaca caatctcaac
     3961 gaagtcattg acgcctgtct ggcggtattg cagaacgtag acatcactat cgatgaactg
     4021 atcgacatcg tgccggcacc ggacttcccg actgccggca tcatctacgg cactgttggc
     4081 gtgaaagaag gttaccgcac cggccgcggc cgcgtggtga tgcgcggccg cacccatttt
     4141 gaagacatcg gcaagggcga ccgccaagcg attatcgttg acgagttgcc gtaccaggtc
     4201 aacaagaaaa ccctgatcga gaagatcgcc gagctggtca acgagaagaa gatcgacggg
     4261 atctccgatc tacgcgacga atcggacaaa tccggcatgc gtatggtgat cgagctcaag
     4321 cgcggcgaag tgccggaagt ggtgctcaac aatctgtaca agcagacgca gttgcaggat
     4381 accttcggca tgaacatggt ggcgctggtc gacggccagc cacgcctgct caacctgaag
     4441 cagatgctgg aagccttcct gcgccaccgc cgcgaggtag taacgcgccg aactgtattc
     4501 gaacttcgca aggcgcgcaa ccgtggccat gtgctggaag gcttggcggt ggcactggcc
     4561 aacgtggacg aaatgatcgc gctgatcaag gccgcaccga caccgccgga tgccaaggtt
     4621 gcgctgatgg cgcgatcatg gaagtcgccg gtggtggagg agatgctgaa gcgcgccgca
     4681 gccgatgcct cgcgtccgga gggactggat ccggcttatg gtctgcaggc caacggctac
     4741 aagctgtccg acgtgcaagc gcaggaaatc ctgcaaatgc gcctgcagcg cctgaccgga
     4801 ctggaacagg acaagatcgt ttccgaatac aaggaagtga tcgacgttat taccgacctg
     4861 ctggacatcc tggccaagcc ggagcgcgtc accgaaatca ttgccggcga actggccgcg
     4921 atcaggcagc agtttggcga caagcgccgc agcgagatcg aacacaacgc ccaggacatt
     4981 tcactggaag atctgattac gccgcaggac gtggtggtga cgctgtcgca caccggttac
     5041 gtgaagtcgc agccgctgga cgaataccgt gcccagcgcc gcggcgggcg cggcaagcag
     5101 gccgccgcaa ccaaggaaga tgacttcatc gacaagatgt tcgtcgccaa tacccacgat
     5161 tacatcctgt gcttctccag ccgcggtcgc gtctactggc tcaaggtcta cgaagtgccg
     5221 cagggaagtc gcatcagccg cggcaagcct attgtcaacc tgttcccgct ggaagaaggc
     5281 gagaagatca acgccatcct gtcagtcaag gaattcgacg agaaccattt catcttcatg
     5341 gcgaccgcgc agggcacggt aaaaaagacc gcgctggtcg agttctccaa cccgcgcaag
     5401 gccgggatca ttgccatcaa cctggacgat ggcgattact tgatcggcgc cgaagtgacc
     5461 aatggcgcca atgacgtggt gctggtgtcc gacggcggca aggcggtgtg gttcgacgag
     5521 gaagacgtgc gccacatggg ccgcgccacc cgaggcgtgc gcggcatgaa gctgcaagcc
     5581 aagcagcagg tgctatccct gttgatcgcc gaacacgaca agcagaccgt gctggtcgcc
     5641 accgaaaacg gttatggcaa gcgcaccgtg ctggccgact tccgtcactc cgggcgtggc
     5701 acgcaagggg taatcgctat cgccacctcg gcacgcaatg gcaaggttgt
//
LOCUS       DAOMFA010000104         5695 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_1817_length_5695_cov_5.445035, whole genome shotgun sequence.
ACCESSION   DAOMFA010000104 DAOMFA010000000
VERSION     DAOMFA010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 5695)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5695)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5695
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1817_length_5695_cov_5.445035"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="PKW44_06200"
     CDS             complement(<1..222)
                     /locus_tag="PKW44_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQR51210.1"
                     /translation="MAGSTARIAFASFIGTAIEFYDFYIYGMAAALVIGSVFFPDSEP
                     AAQALNAFLTFGLAFLARRLGAVLFGHFGV"
     gene            complement(225..2006)
                     /gene="mutL"
                     /locus_tag="PKW44_06205"
     CDS             complement(225..2006)
                     /gene="mutL"
                     /locus_tag="PKW44_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HQR51211.1"
                     /translation="MSVIHLLPDHLISQIAAGEVVERPASALKELLENSLDAGADDIT
                     VHLQQGGVKQLRVADNGAGIAPDDLTLALTRHATSKITSLNDLENVASLGFRGEALAS
                     IASVSRTTLASRVKSERHAWRVAAEGGSITAREPAALDAGTVVEVSDLYYNTPARRKF
                     LKTEATEYGHCEEVFKRIALSRPQAGFLLQHNGRAQWRLAAGEPRKRFAEILGEEFAE
                     HALLLDESAAGLRLWGMVAKPAYSKLSRDAQYFYVNGRFIRDKLVAHAIRQAYQDVLH
                     HERHPAFVLFFELDPTLVDVNVHPTKTEVRFRDSQAVHRFIFHALHKALAAPVGAAQP
                     DASLPQTGASPSAGGGSFYPGYQPQIPLRANEGLGFYQTLFGSQPPSTPLQAGGVPSA
                     GADETEQEYPLGFAIAQLHGVYVLSQNRQGLVVVDMHAAHERIMYEQLKTALDTHAVA
                     TQPLLLPVSFNADRLEVATAEEQGAVLRDLGFEIATLSPATLVVRAVPAMLADADAVA
                     LARAVLADIREFGGSRVLTERRNELLATMACHAAVRANRSLTLTEMNALLRDMEATER
                     SEQCNHGRPTWLQVGMADLDKMFMRGK"
     gene            complement(2094..3419)
                     /locus_tag="PKW44_06210"
     CDS             complement(2094..3419)
                     /locus_tag="PKW44_06210"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HQR51212.1"
                     /translation="MLRAFISILLLSITLAAQAANSVVSARIWPAADYTRITLESPKP
                     IVHRMQTLKDPERLVLDLESVEINAVLKSLAGKILFSDPYIKQVRIANFKPGVVRMVI
                     DLKAEIKPSLFALQPAGEYQHRLVLDVYPAQDPLMAMLQEREKSSQPTESGGYVPEEQ
                     PRMAAVLSTPHQEPKAADMEQSMQGAAGSETENAPADGGKSEVMRLVTIAIDAGHGGE
                     DPGARGATGTHEKDVTLSIAKRLKAMVDAEPNMRGVLTRDGDFFIPLHGRVIKARKLQ
                     ADLFVSIHADAFVRPEARGSSVFALSERGASSASARYLAKRENESDLIGGVSLDDKDP
                     YLARTLLDLSQTATISDSLKLAKAVLGQLGEINDLHKRSVEQAGFAVLKSPDIPSILV
                     ETAFISNPEEEKRLNDDEYQDKLANAILSGIKRYFSSNPPLAKSKLALQ"
     gene            complement(3386..3868)
                     /gene="tsaE"
                     /locus_tag="PKW44_06215"
     CDS             complement(3386..3868)
                     /gene="tsaE"
                     /locus_tag="PKW44_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HQR51213.1"
                     /translation="MSHNPDHITLALGDEAATLALGASLAKVLQGGLSIWLQGNLGAG
                     KTTLVRGLLRGLGFLGKVKSPTYTLVEPYVISGLYLYHFDLYRFADSEEWDAAGFRDY
                     FNTQSVCLVEWPDRAGEMLPPADIEIHLTPYGIGRRAELHAGTRIGKQCLERLSRFCY
                     "
     gene            3860..4939
                     /gene="queG"
                     /locus_tag="PKW44_06220"
     CDS             3860..4939
                     /gene="queG"
                     /locus_tag="PKW44_06220"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HQR51214.1"
                     /translation="MGHDSYDLRQLAANIKSWGKALGFDQVGITDTDLSTAEPYYREW
                     LQSGFHGAMDYMAKHGTKRTRPSELVPGTLRIISARMDYQADNTKDSWEVMQDSRQAF
                     ISRYALGRDYHKVMRARLQTLCDRIADEIGTFGYRVFSDSAPVLEVQLAQKAGLGWRG
                     KHTLLLSREAGSWFFLGEIYTDLPLPVDKPGQNHCGTCRACLDICPTQAIVAPYSVDA
                     RRCISYLTIELKGSIPPDLRPLIGNRVYGCDDCQLACPWNKFAQVSKEPDFHVRHGLD
                     NVTLLELFRWKEEEFMTRMAGSAIYRIGWEQWLRNIAVGLGNAPASSAVMAALQQRAD
                     HPSALVREHVAWALQQHGAIAAGEN"
     gene            complement(4936..>5695)
                     /locus_tag="PKW44_06225"
     CDS             complement(4936..>5695)
                     /locus_tag="PKW44_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FimV/HubP family polar landmark protein"
                     /protein_id="HQR51215.1"
                     /translation="DFGLSGDASTIISPAVAEPASLLASNAIEGPEISFDLPQIGDVL
                     LQPPADDVKIDAPASVSSREEVPAVTMKDLEQAMHFDSLLNEEAGGPEQIVLEPAAEE
                     PAASSELIDFSFDLDQTVSAGADKKAEPELPAASLDIDLSGISLDFEESAPSDLPAPH
                     FEMPAKQADEIFPKVDMAAFGLEENQAAGTALQPSLGGAESESSDVETKLDLVTAYLD
                     MGDTAGARELLDEVLKEGGPGQRRRAQALVESIG"
BASE COUNT         1159 a         1676 c         1708 g         1152 t
ORIGIN      
        1 aacaccgaaa tgcccgaaca acaccgcgcc caggcgacgg gcgagaaagg caaggccaaa
       61 agtcaggaaa gcatttagcg cctgcgccgc cggttcactg tccggaaaga acacagaccc
      121 gatcaccagc gccgccgcca tgccgtagat gtagaaatcg tagaactcga tggcggtgcc
      181 gatgaagctg gcgaaggcga tgcgagcagt ggaaccagcc atggctactt accgcgcatg
      241 aacatcttgt ccaaatcagc catgccgacc tggagccagg tcgggcggcc gtggttgcat
      301 tgttccgagc gttccgtggc ttccatatca cgcagcaggg cgttcatttc ggtgagggtg
      361 aggctgcggt tggcgcgcac ggcggcgtgg caggccatgg tggcaagcag ttcgttgcgg
      421 cgttcggtga gcacgcggct gccgccgaat tcgcggatgt cggccagcac cgcccgcgcc
      481 agggcgacgg catcagcatc ggccagcatg gcggggacgg cgcgcaccac cagcgtggcg
      541 ggcgatagcg tggcgatctc gaaaccgagg tcgcgcagca cggcgccctg ctcttccgcc
      601 gtggcgactt ccagccggtc agcattgaag ctgaccggga gcaacagggg ctgggtggcg
      661 acggcgtggg tgtccagcgc ggtcttgagt tgctcgtaca tgatgcgttc gtgcgcggcg
      721 tgcatatcca ccaccaccag cccttgccgg ttttgcgata acacgtacac gccgtgcagc
      781 tgggcgatgg cgaagccaag cgggtattcc tgttcggttt cgtccgctcc agcgcttggc
      841 actccccctg cttgcaaagg cgtagagggt ggctgactgc cgaacagggt ttggtagaaa
      901 cccagcccct cgttagcccg gagcgggatt tgcggctggt agccggggta gaaactgcca
      961 cctccggcgc ttggcgacgc cccggtttgc ggcagtgagg catcgggttg cgcagcgccc
     1021 accggcgcgg ctagtgcctt atgcagggcg tggaagataa agcggtgcac tgcctgcgaa
     1081 tccctgaacc gcacctcggt cttggtcggg tggacgttga catccaccag cgtcgggtct
     1141 agctcgaaga acagcacgaa ggcggggtgc cgctcgtggt gcagcacgtc ctgataggcc
     1201 tggcggatgg catgggcgac cagtttgtcg cggatgaaac ggccgttgac gtagaaatac
     1261 tgcgcgtcgc gcgacagctt ggaataggcc ggcttggcca ccatgcccca caggcgcagg
     1321 ccggcggcgg attcgtccag caacagggcg tgttcggcga attcctcgcc caggatttcg
     1381 gcaaagcgct tgcgcggctc gcccgccgcc agccgccatt gggcgcggcc gttgtgctgc
     1441 agcaggaagc cggcttgcgg acgcgacagc gcgatgcgct tgaagacctc ctcgcagtgg
     1501 ccgtattcgg tggcctcggt tttgaggaat tttcttctgg ccggagtgtt gtaatagagg
     1561 tcgctgactt ccaccacggt gccggcatcc agcgcagccg gttcgcgcgc cgtgatgctg
     1621 ccgccctctg ccgccacgcg ccaggcgtgg cgctcgctct taacgcggct ggcaagcgtg
     1681 gtgcgcgaca ccgaagcaat gctggccagc gcctcaccgc ggaaaccaag actggcgaca
     1741 ttttccaggt cattcaggct ggtgatcttg ctggtggcat ggcgcgtcag cgccagagtg
     1801 agatcatccg gcgcgatgcc ggcaccgtta tccgccacac gcaactgctt gacgccgcct
     1861 tgctgcaaat ggacggtaat gtcgtcggcg cccgcatcca ggctgttttc cagcaactcc
     1921 ttcagcgccg aggcgggccg ctcgacgact tcgccggcgg cgatctggct gataagatgg
     1981 tcggggagaa gatgaatgac ggacataatc cggtcatttt acctgcttaa tcactggtaa
     2041 cgcagggctt cgatcagcag cggcgaactg actttggggc gactgcctga aagctactgc
     2101 agcgcaagct tggattttgc cagcggagga ttcgaggaga aatagcgctt gattccagac
     2161 aggatggcgt tggccaactt gtcctgatat tcatcatcgt tgagacgttt ttcttcctcg
     2221 ggattactga taaaggctgt ctcgaccaga atggaaggga tgtcgggtga tttcagcaca
     2281 gcaaagccag cctgctccac cgagcgttta tgcagatcat tgatctcgcc caattgcccc
     2341 agcaccgcct tggccaactt gaggctgtcg ctaatggtag ccgtttgcga caaatccagc
     2401 agcgtcctgg caaggtaggg atccttgtca tccagactga caccgccgat caggtcggac
     2461 tcgttttcgc gctttgccag gtagcgtgca gaggcgctgg aggcgccacg ctccgacagt
     2521 gcaaacaccg aagaaccacg cgcttccggc cggacaaagg catcggcatg aatggaaaca
     2581 aacagatctg cttgcagctt gcgtgccttg atcacgcgtc catgcaacgg aataaagaaa
     2641 tccccatcac gcgtcagcac gccgcgcatg ttgggttcgg catccaccat agccttgagc
     2701 cgcttggcga ttgacagggt gacgtctttt tcgtgagtac cggtagcgcc cctggcaccc
     2761 gggtcttctc ccccatgccc agcatctata gcgatggtga ccaagcgcat cacctcggac
     2821 ttgccgccat cagcgggggc gttttcagtc tcgctcccgg ccgcaccctg catggactgc
     2881 tccatgtcgg cagccttggg ttcctggtgt ggtgtggaaa gcacagcagc catgcggggt
     2941 tgttcctccg gtacatagcc gccactttcg gtaggttgtg atgacttctc gcgttcctgc
     3001 aacatggcca tcagcggatc ctgcgcaggg taaacgtcca gcaccagccg atgctgatat
     3061 tcacccgccg gttgcagggc gaacagggat ggttttattt ccgccttcag gtcaatcacc
     3121 atgcgcacca cgcccggctt gaagttggcg atgcgcacct gcttgatgta gggatcgctg
     3181 aagagaattt ttccggccag ggatttcagc accgcattga tctcgacgct ttcaagatcc
     3241 agcaccaggc gctcgggatc cttgagggtc tgcatcctgt ggacgatggg cttgggagat
     3301 tccagcgtga tacgggtgta atcggcagcg ggccagatgc gtgcggaaac aacggaatta
     3361 gccgcctgcg cggccagagt gatgctcaat aacagaatcg agataaacgc tctaagcatt
     3421 gctttccgat ccttgttcct gcatgaagtt ctgcgcggcg cccgattccg tacggtgtga
     3481 ggtggatttc gatatcagct gggggcaaca tttcgccagc cctgtccggc cattcgacca
     3541 gacatacaga ctgcgtattg aagtaatcgc gaaaccctgc ggcatcccac tcctccgagt
     3601 ctgcaaatct gtataaatca aagtgataca agtataaccc agaaattacg taaggttcaa
     3661 ccagcgtata ggtcgggctt tttaccttgc ctaaaaaacc caagccgcgc agcaggccac
     3721 gcacgagcgt ggtcttgcct gcaccaaggt tgccttgcag ccaaatgctc aagccgccct
     3781 gtaacacttt ggcaagactg gctcccagcg ccagagtagc ggcttcgtca ccgagcgcaa
     3841 gtgttatatg atcggggtta tgggacatga ttcatacgat ctaaggcaac ttgccgccaa
     3901 cattaagagt tggggcaagg cgctcggttt tgaccaggtc ggcatcaccg acactgactt
     3961 gagcacggcc gaaccgtact accgggaatg gctgcaatcg ggtttccatg gcgccatgga
     4021 ttatatggca aaacatggca ccaagcgcac gcggccgtcg gaactggtac ccggcacgct
     4081 acgcatcatc tcggcacgca tggactacca ggctgataac acgaaagaca gctgggaagt
     4141 catgcaagac agccgccaag cgtttatctc acgctatgcc ctggggcggg attaccacaa
     4201 ggtgatgcgc gcacgcctgc aaaccctgtg tgaccgtatc gctgatgaga tcggaacctt
     4261 cggctaccgt gtatttagcg acagtgcgcc ggtgctggaa gtgcaactgg cgcaaaaggc
     4321 cgggctgggc tggcgcggca agcacaccct gctgctctct cgtgaagccg gctcctggtt
     4381 cttcctggga gaaatctata ccgatctgcc gctgccggtg gacaaacctg ggcagaatca
     4441 ttgtggaacc tgccgcgcct gcctcgatat ttgccccacc caagctatcg tcgcgcccta
     4501 ttcagtggat gccagacgct gcatctccta cctcaccata gaactcaaag gtagcattcc
     4561 gccggacttg cgccccctga tcggtaaccg tgtttatggt tgtgatgact gtcagttggc
     4621 ctgtccatgg aacaaatttg cgcaggtctc caaggaaccg gactttcatg tccgccacgg
     4681 actggacaat gtaacgctgc tggaactatt ccgttggaag gaagaggaat tcatgaccag
     4741 gatggccggc agcgcgattt accgtattgg ctgggaacaa tggctgcgca atatcgcggt
     4801 aggactgggc aacgcccctg cttcatctgc agtgatggcg gcgctgcaac agcgtgccga
     4861 ccacccatca gcgctggtca gggagcacgt ggcatgggca ctgcaacagc atggcgccat
     4921 cgccgccggc gagaattagc cgatcgattc cactagcgcc tgtgcacgcc tgcgttgccc
     4981 cgggccgccc tccttgagca cctcgtcaag caactcacgc gcaccggccg tatcgcccat
     5041 atccagatag gcggtaacca ggtccagctt ggtctcgacg tcggacgact cagactctgc
     5101 gcccccgagg gatggctgaa gcgcggttcc agcggcctgg ttttcttcta acccgaacgc
     5161 tgccatgtca actttcggaa aaatttcatc agcctgcttt gctggcatct caaagtgcgg
     5221 ggcaggcaaa tcgctaggtg ccgattcttc gaaatcgaga ctgattcctg aaagatcgat
     5281 atccagcgat gctgctggca gttcaggttc ggccttcttg tcggcgccag cggaaactgt
     5341 ttgatccaga tcgaagctga aatcaatgag ttcactactg gctgcaggct cttccgcagc
     5401 gggttcgagt acgatctgtt cagggccacc cgcctcctca tttaataaag agtcgaagtg
     5461 catggcctgc tccaggtcct tcatcgttac agcgggaact tcttctcgtg acgatacgga
     5521 cgctggggcg tcaatcttga cgtcatcggc tggcggctgc aacaggacat ccccaatctg
     5581 tggaaggtcg aaggatatct ccgggccttc aatagcattg cttgccaaaa gtgaagccgg
     5641 ctcggctacg gctgggctga taatagtcga agcatcacca gacaagccaa aatcc
//
LOCUS       DAOMFA010000105         5566 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_1880_length_5566_cov_5.675377, whole genome shotgun sequence.
ACCESSION   DAOMFA010000105 DAOMFA010000000
VERSION     DAOMFA010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 5566)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5566)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5566
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1880_length_5566_cov_5.675377"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1062)
                     /locus_tag="PKW44_06230"
     CDS             complement(<1..1062)
                     /locus_tag="PKW44_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HQR51216.1"
                     /translation="MNEQHADYLGPILGRYGRSATNLMQILREAQEHFGWLHPDAIDH
                     LTCQLKVPRTKIEGVASFYSFFYLEPRGKYRVLFSDNITDRMLGNQALMERMCRNLWV
                     EPGKMSEDGLVSVGTTSCTGMCDQGPAILVNNIAITRMTEQRVDEICELIRQSVPMSD
                     WPAEYFQIIDNIRRKDMLLEDGFVTGSALKAAFARGAEGMLAEVKASGLRGRGGAGFS
                     TGMKWEFSRNAPGETHYVVCNADEGEPGTFKDRVLLTSYTDQVFEGMTLCGYVIGAKK
                     GMLYLRGEYRYLLDQLNAVLARRRQQNLLGANVLGQTGFDFDIEIHLGAGAYICGEES
                     ALIESPEGKRGTPRNRPPFP"
     gene            1146..2036
                     /locus_tag="PKW44_06235"
     CDS             1146..2036
                     /locus_tag="PKW44_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HQR51217.1"
                     /translation="MTTARIFDKLAIFGVGLIGGSVALALKRAGQTGTIVGVGRHHDA
                     MQEALHLGVIDQTASAAEAAKDADLILLATPVGQFPAILQTIAPHLKSTAVITDAGST
                     KGDVVAAARTNLGEFLPRFVPAHPIAGAEKSGVLAAKAELFDSKKVVLTPLPETDPDA
                     TAKAEAFWRACGARVSDMTVETHDRIFAAVSHLPHVLAFALVEDIACRPNADQLFGFA
                     AGGFRDFSRIAGSSPEMWRDICLANRDAMLQELDAYATQLAQIRSLIDSNDGVELAKL
                     FEHARNARSAWADAQQKQKK"
     gene            2041..3321
                     /gene="aroA"
                     /locus_tag="PKW44_06240"
     CDS             2041..3321
                     /gene="aroA"
                     /locus_tag="PKW44_06240"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020163940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HQR51218.1"
                     /translation="METLDLSPASKAAGTIKLPGSKSISNRTLLLAALAEGITQVRDL
                     LASDDTARMLEALKILGVPLEQTGPDDWKVGGQAGPFKVKDADLFLGNAGTAFRPLTA
                     ALALSGGHYQLSGVPRMHERPIGDLVDALRQAGADIRYLGNADYPPLEILPAVVNSGK
                     VNVKGNVSSQFLTALLMALPLTGQQTIIEVVGELISKPYIEITLNLMARFGVVVKRDG
                     WQAFTIPANSHYTSPGELWVEGDASSASYFLAAGAIGGGPVRVEGIGKESIQGDVRFA
                     EALEKMGARITMGNHWIEASASGRLNAIDLDCNHIPDAAMTLAIAALFADGTTTLRNI
                     ASWRVKETDRIAAMASELRKIGAVVEEGADYICVTPPQKLTPNAAIDTYDDHRMAMCF
                     SLVSLGGVPVTINDPRCVAKTFPDYFARFAQVVA"
     gene            3318..3974
                     /gene="cmk"
                     /locus_tag="PKW44_06245"
     CDS             3318..3974
                     /gene="cmk"
                     /locus_tag="PKW44_06245"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HQR51219.1"
                     /translation="MNMIPVIAIDGPSASGKGTVAQQVARRLGYHYLDSGALYRIVAL
                     AGLKQGISWQDEAALAQVAVGLAIHFDNGEIFLNGENVSDAVRSEEMSRGASEVAVHP
                     AVRSALFELQRSFRKAPGLVADGRDMGSVVFPDAATKIFLTASAEVRAERRYKQLMEK
                     GIAANMADILQDLQARDTRDRARIVAPLQQCADAILLDTDHLTIEQAVQAVLDFQSRH
                     "
     gene            4062..>5566
                     /gene="rpsA"
                     /locus_tag="PKW44_06250"
     CDS             4062..>5566
                     /gene="rpsA"
                     /locus_tag="PKW44_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HQR51220.1"
                     /translation="MATAAPQESFAALFEESLARQEMRSGEVITAEVISVDNDFVVVN
                     AGLKSESVIPTSEFRDDKGELEVAVGDFVKVAIDALENGFGETKLSREKAKRLAAWLD
                     LEDAMNEGRIIKGLINGRVKGGLTVMVNGIRAFLPGSLVDTRPVKDTTPYEFKEWDFK
                     VIKLDRKRNNVVVSRRAVMEVSMGADREALLANLKEGAVVKGIVKNITDYGAFVDLGG
                     IDGLLHITDLAWRRVKHPSEVLTVGEEVEAKILKFDQEKNRVSLGIKQLGDDPWVAMS
                     RRYPVSTRLFGKVTNLTDYGAFVEIEPGIEGLVHVSEMDWTNKNVHPAKIVQLGDEVE
                     VMILEIDEDRRRLSLGMKQCQSNPWDDFAASHKKGDKVSGQIKSITDFGVFIGLPGGI
                     DGLVHLSDLSWSQTGEEAIRNFKKGDELEAVVLSIDVEKERISLGVKQLDNDPFSSYT
                     TTHDKGALVKGTVKSLDAKGAVITLEGEIEGYLRASEVSREKVEDIRNHLK"
BASE COUNT         1157 a         1743 c         1629 g         1037 t
ORIGIN      
        1 cgggaacggc ggccggttgc gcggcgtgcc gcgcttgcct tccggcgatt cgatcagcgc
       61 cgattcttcg ccgcagatgt aggcgccggc gccgagatgg atttcgatgt cgaaatcgaa
      121 gccggtctgg ccgaggacat tggcgccgag caagttttgt tggcgcctgc gcgccagcac
      181 ggcgttgagc tgatccagca ggtagcggta ttcgccgcgc aggtagagca tgcctttttt
      241 cgcgccgatg acgtagccgc acagggtcat gccctcgaac acctggtcgg tgtaggaagt
      301 cagcagtacg cggtccttga aggtgccggg ctcgccttcg tcggcgttgc agacaacata
      361 atgggtttca cccggcgcgt tacggctgaa ttcccacttc atgccagtgg aaaacccggc
      421 gccgccgcgt ccgcgcaggc cagaggcctt gacctcggcc agcataccct ctgcgccgcg
      481 tgcgaacgca gccttgagcg ccgagcccgt cacgaagccg tcctccagca gcatgtcctt
      541 gcgccggatg ttgtcgatga tctggaagta ctcggccggc cagtcactca tcggcacgga
      601 ctggcggatc agctcgcaga tttcgtccac gcgctgctcg gtcatccggg tgatggcgat
      661 gttgttgacc aaaatggccg gcccctggtc gcacatgccg gtgcaggagg tggtccccac
      721 gctgaccagg ccgtcttccg acatcttgcc tggctccacc cacaaattgc ggcacatgcg
      781 ctccatcagc gcttgattgc ccagcatgcg gtcggtgatg ttgtcggaga acagcacgcg
      841 gtacttgccg cgcggctcca ggtaaaaaaa ggaatagaag ctggccacgc cttcgatctt
      901 ggtacgcggc accttgagct ggcaggtgag atggtcgatc gcatcgggat gcagccagcc
      961 aaagtgctcc tgcgcctcgc gcagtatctg catcagattg gttgccgaac gaccgtaacg
     1021 ccccaagatg gggcccaggt agtctgcgtg ttgttcgttc atgattcctc tgcttgtagt
     1081 gcaattatac gccacctcgc accaacagat aagcgagttc tgcgtgctac aatacgcgcc
     1141 attttatgac aaccgcgcgc attttcgaca agctcgccat cttcggcgtc ggcctcatcg
     1201 gcggctcggt cgcgctggcc ctgaagcgcg ccggccagac cggtaccatc gttggtgtcg
     1261 ggcggcacca tgatgccatg caagaagcac tccatctcgg cgtcatcgac cagacggcca
     1321 gtgctgccga ggcagccaag gacgccgacc tgatcctgct ggccaccccg gtcggccagt
     1381 tcccggccat cctgcaaacg atcgccccgc acctgaaatc cacggccgtc attaccgatg
     1441 ccggcagcac caagggtgac gtggtcgccg ctgcgcgcac caatcttggc gagttcctgc
     1501 cgcgttttgt gccggcccat cccatcgccg gggcagaaaa aagcggcgtt ctggcagcca
     1561 aggcggagtt attcgacagc aagaaagtag tgctgacccc tttgcctgaa accgaccctg
     1621 atgcaaccgc caaggccgaa gcgttctggc gcgcctgtgg cgccagagtc agcgacatga
     1681 cggtggaaac ccatgaccgt attttcgccg ccgtcagtca tttaccccac gtactagcct
     1741 ttgcgctggt agaagacatc gcgtgccgcc ccaatgccga ccaactgttc ggcttcgccg
     1801 ccggcggctt ccgcgatttc tcgcgtatcg ccggcagctc gccggaaatg tggcgcgata
     1861 tctgcctcgc caaccgcgac gccatgttgc aggagctgga tgcctacgcc acccagctcg
     1921 cgcaaatccg cagtctgatc gattccaatg acggcgtgga actggccaaa ttattcgaac
     1981 acgcccgcaa cgcacgtagc gcctgggcgg acgcccagca aaagcaaaag aaataaaata
     2041 atggaaacgc tcgacctttc ccccgccagc aaggctgctg gcacgataaa actgcccggt
     2101 tccaagagca tctccaaccg caccctgctg ctggcagcgc tggccgaagg catcacgcag
     2161 gtccgtgacc tgctggcctc ggacgacacc gcgcgcatgc tggaagcgct gaaaatcctg
     2221 ggcgttccgc tggagcagac cgggccggac gactggaagg tcggcggaca ggccgggcca
     2281 ttcaaggtta aagatgctga tttatttctc ggcaacgccg gcaccgcctt ccgcccgctg
     2341 accgccgcgc tggcattgtc cggcggccac taccagctgt ccggcgtgcc acgtatgcac
     2401 gaacgcccca tcggcgacct ggtggatgca ctgcgccagg ctggcgccga tatccgctat
     2461 ctgggcaacg cggactaccc accgctggaa atcctgcctg ccgtcgtgaa cagtggcaaa
     2521 gtcaacgtta agggcaacgt ttccagccaa ttcctcaccg cgctactgat ggcgctgccg
     2581 ctcaccggcc agcagaccat catcgaagtc gttggcgaac tgatctctaa gccctatatc
     2641 gaaatcacgc tcaacctgat ggcgcgcttc ggtgtcgtag tcaagcgcga tggctggcag
     2701 gcgttcacca tcccggctaa cagtcactac accagccctg gcgaactgtg ggtagaaggc
     2761 gacgcctcct cggcttccta tttcctcgcc gctggcgcta ttggcggcgg cccggtacgg
     2821 gtagaaggga taggtaaaga gagcattcag ggcgatgtgc gcttcgccga agcgctggaa
     2881 aaaatgggcg cgcgcatcac catgggcaac cactggatcg aagccagcgc cagcggcaga
     2941 ctgaatgcca tcgacctcga ctgcaaccac atcccggacg ctgccatgac cctggccatc
     3001 gccgcactgt tcgccgacgg caccactacc ctgcgcaaca tcgcgagctg gcgcgtcaag
     3061 gaaaccgacc gcatcgccgc catggcgagc gagctgagaa aaatcggagc agtggtggaa
     3121 gagggcgcag actatatctg cgtcacgcct ccgcagaaac tgacccccaa cgccgccatc
     3181 gatacctacg acgaccaccg catggcgatg tgcttttcct tggtcagcct gggcggggtt
     3241 ccggtcacca tcaacgaccc caggtgtgtg gccaagacct ttccggatta tttcgcgcgc
     3301 ttcgcgcagg tcgtcgcatg aacatgattc ccgtcattgc catcgatggt ccctccgcct
     3361 cgggaaaagg cactgtcgca caacaggtag cgcgcaggct gggctaccac tatctggatt
     3421 ccggtgcgct gtatcgcatt gtggcgctgg ccggactgaa gcaaggcatt tcctggcagg
     3481 acgaggccgc gctggcacag gtagccgtcg ggttggccat tcatttcgat aacggcgaaa
     3541 ttttcctcaa tggggaaaat gtctcagatg ccgtgcgttc cgaagaaatg agccgcggtg
     3601 cctcggaagt ggccgtgcat cccgccgtgc gcagtgcctt gtttgaattg cagcgcagct
     3661 tccgcaaggc gccggggctg gtagcagatg gccgcgacat gggctcagtg gtattcccgg
     3721 atgctgccac caagatattc ctcaccgcca gcgccgaagt gcgtgcggaa cgtcgatata
     3781 agcagttgat ggaaaaagga atcgctgcta atatggcgga catcttgcag gatctgcaag
     3841 cgcgcgatac gcgcgaccgc gccagaatag tggctccgtt gcagcaatgt gcagatgcaa
     3901 tactgctcga tacagaccat ctgaccatcg agcaagctgt acaggcggta ctggatttcc
     3961 agtcccgcca ttagttttta agtatcacgg atcgcctgat tacgtgatgt ttttcaacca
     4021 accccgccgc caggtgggat tttttgttag gtatgttatt aatggctact gctgcccccc
     4081 aagaaagttt tgccgccctg ttcgaagaaa gcctggctcg ccaggaaatg cgttcgggcg
     4141 aagtcatcac cgctgaagtc atctccgttg acaacgattt cgtcgtcgtc aacgccggcc
     4201 tcaaatccga aagcgtgatc cccacctccg aattccgcga cgacaaaggc gagctggaag
     4261 tcgctgtcgg cgatttcgtc aaggtcgcca tcgacgccct ggaaaacggc ttcggtgaaa
     4321 ccaagctttc gcgcgaaaag gccaagcgcc tcgctgcgtg gctggatctg gaagacgcaa
     4381 tgaacgaagg ccgcatcatc aagggcctca tcaacggtcg cgtcaagggc ggcctgactg
     4441 tcatggtcaa cggcatccgc gccttcctgc cgggctcgct ggtggatacc cgcccggtca
     4501 aggacaccac cccttacgaa ttcaaggaat gggacttcaa ggtcatcaag ctcgaccgca
     4561 agcgcaacaa cgtggtggtt tcccgccgcg cggtgatgga agtctccatg ggcgccgacc
     4621 gcgaagcact gctggccaac ctgaaggaag gcgctgtggt caagggcatc gtcaagaaca
     4681 tcaccgacta tggtgcattc gttgacctgg gtggcatcga cggcctgctg catatcaccg
     4741 acctggcctg gcgccgcgtc aagcacccgt ccgaagtgct caccgttggc gaagaagtcg
     4801 aagccaagat cctcaaattc gatcaggaaa agaaccgcgt ctccctgggc atcaagcaac
     4861 tgggcgacga tccgtgggtt gccatgagcc gccgctaccc ggtcagcacc cgcctgttcg
     4921 gtaaggttac caacctgacc gactacggcg cgttcgtcga aatcgaaccg ggcatcgaag
     4981 gcctggtcca cgtttccgaa atggactgga ccaacaagaa cgtgcatccg gccaagatcg
     5041 tccagctggg cgacgaagtc gaagtcatga tcctggaaat cgacgaagat cgccgtcgtc
     5101 tgtccctagg catgaagcaa tgccaatcca atccgtggga cgacttcgct gccagccaca
     5161 agaagggtga caaagtcagt ggccagatca agtcgatcac cgacttcggc gtcttcatcg
     5221 gcctgccggg cggcatcgac ggcctggtcc atctgtccga cctgtcctgg agccaaaccg
     5281 gcgaagaagc catccgcaac ttcaagaagg gtgacgaact ggaagccgtg gtgctcagca
     5341 tcgacgtcga gaaggagcgc atctctctgg gcgtgaagca gctcgacaac gatccgttca
     5401 gctcctacac caccacccac gacaagggtg cactggtcaa gggcacagtc aagagccttg
     5461 atgccaaggg tgccgtgatt acgctggaag gcgagatcga aggctacctg cgtgcttccg
     5521 aagtttcccg cgagaaggtg gaagacatcc gcaaccacct caagga
//
LOCUS       DAOMFA010000106         5532 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_1908_length_5532_cov_5.624247, whole genome shotgun sequence.
ACCESSION   DAOMFA010000106 DAOMFA010000000
VERSION     DAOMFA010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 5532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5532
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1908_length_5532_cov_5.624247"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..577
                     /locus_tag="PKW44_06255"
     CDS             <1..577
                     /locus_tag="PKW44_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HQR51221.1"
                     /translation="AETEKYPHVTFFFNGGEETAYPGEDRILVPSPHVATYDLKPEMS
                     AFEVTDKLEQAILSQQYDVIICNYANADMVGHTGILPAAIKAIEAVDACVGRVVKAMR
                     SIGGEVVITADHGNAECMEDHANRQPHTQHTTNLVPLLYVGRPAQLAPTGALSDVAPT
                     LLHLMGLPQPAEMTGHTLVDSADMPVAANAR"
     gene            579..1784
                     /locus_tag="PKW44_06260"
     CDS             579..1784
                     /locus_tag="PKW44_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020183888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HQR51222.1"
                     /translation="MRAHQRCALALTFALFFTPFPADAGKQEAARESLEQLQNRIESL
                     KKEMEKTEGAHAEAADALKQSEQAISEANRKLYELNQQHRENLATLQTLQQQKSGLEQ
                     TIGEQQRRLGELLYRQYLNSQQSYLQIVLTAHDPATIARDLYYLGYVARARADLIASL
                     RENLGQVAVLNEKTTSTLDQIAELKSEQENQRKELQTRQKARKSLLNKLSTQIKSQRS
                     EITKLKRDEKRLSNLVERLARIVPKTPKAKPASREKPRLNEAVPTTDPDSGSFAALKG
                     KLRLPIRGEIANRFGTPREDSGVFWKGLFIRSGEGTEVKSVAGGRVVFADWLRGFGNL
                     LIVDHGDGYMSLYGNNQALLKKAGEEVSAGDALATVGSSGGSAESGLYFELRYQSKPL
                     DPLAWSVTR"
     gene            1810..2535
                     /locus_tag="PKW44_06265"
     CDS             1810..2535
                     /locus_tag="PKW44_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011310944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HQR51223.1"
                     /translation="MIFLFTDFGASDLYVGQMKARLLTANPDARMVDLLHQAPNFDVR
                     AGAHLLAALAPQLAHGVTLAVVDAGVGSKRRPVAVQADNGWYVGPDNGLLSVLVARAQ
                     TSRCWEIIWQPPHLSHSFHGRDLFAPIAAMLESSAWWPHALREVSGLNIDLGAADLAE
                     VIYTDHYGNAMTGLRSGHLPQETEIIVAGHRIGYARVFAEAPQGAPFWYENSIGLVEI
                     AVNRGSAAQLLGVGPGSLVRVIA"
     gene            complement(2519..2743)
                     /locus_tag="PKW44_06270"
     CDS             complement(2519..2743)
                     /locus_tag="PKW44_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020183674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HQR51224.1"
                     /translation="MLNLYGTENCHLCEDAQLVLREAGLSWRDVEISEDDALLARYGT
                     KIPVLLRPDSGDELCWPFGVQEVQDFMQSP"
     gene            2858..4294
                     /gene="mgtE"
                     /locus_tag="PKW44_06275"
     CDS             2858..4294
                     /gene="mgtE"
                     /locus_tag="PKW44_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HQR51225.1"
                     /translation="MAENEQSKESLQEILQQVIHLLNKHRLVEGLVHKQDMPRHDLVE
                     SLVHKQNLSELQHKLDELHPADVAYILEALPIEQRLLVWDLVKTERDGDILLEVSDAV
                     RQTLIADMDSDELLAAAEQLDTDEIADLAPDLPRDVLQELLVSLDGQNRERLQSALSY
                     PEDTVGALMDFDIVTIRDDISLEVALRYLRRLGKLPDHTDKLFVVDRDDILRGVLPLK
                     RLVVYDPEASVAAVMADDAVIFHPEEQASDAAQAFRRYDLVTAPVVDSNNKLVGRLTV
                     DAVVDFIQDESESEMLSMAGLREEEDLFSSVWKSVQNRWTWLAINLMTAFIASRVIGV
                     FEGSIEKIVALAALMPIVAGIGGNSGNQTTTMIVRGLALGQISALNMQKLLLKELGVA
                     LLNGLVWGSVLGVAAFLLYHNLPLGLVMTGAMTLNLMLAALMGVGIPLAMTRLGRDPA
                     VGSSVLITAVTDSGGFFIFLGLATFFLL"
     gene            4303..>5532
                     /locus_tag="PKW44_06280"
     CDS             4303..>5532
                     /locus_tag="PKW44_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HQR51226.1"
                     /translation="MGSEIINMWTELAADLRTPAVYGQLIVLVLSLSLAWLLNRILHG
                     QIMHKAPESWKVGIGGAKRLLFPLSSLLFVHLGMLVLRHGGNHTSMLKLASSLLVAMA
                     VIRLTVYTLRYVFAPSNWLRTAESAIVGTVWGLVALHLVGFLPELLRALDEISFTMGK
                     HTISLWLILQAAAIVVVTIILALSASRFLENKLMRAEQIDMNMRVVLSKLLRVVLTLV
                     GVLTALSAVGFDITLLSVFGGALGVGLGLGLQKIASNYVSGFIILLDQSVHMGDVMTI
                     NDHYGVVYQIRSRYLVLRKLDGTEVIIPNETLIASAVINHSLSDRKARILMPVQISYD
                     SPLELAMQAMREVALRQPRVLKDPGPEVLVKGFGESGIDLNLSIWIEDPEEGSGSLQS
                     AIYLGIWREFQQQGVAGP"
BASE COUNT         1156 a         1697 c         1659 g         1020 t
ORIGIN      
        1 cgcggaaacc gaaaaatacc cgcacgtcac cttcttcttc aacggcggag aggagaccgc
       61 ctatcccggt gaagaccgca tcctggtgcc gtcaccacat gtcgccacct acgacctcaa
      121 gccggaaatg agtgctttcg aggtcaccga caaactggag caggccatcc tcagccagca
      181 gtacgacgtc atcatctgca actatgccaa cgccgacatg gtggggcaca ccggcatcct
      241 gccggcagcg atcaaggcca tcgaagccgt ggatgcctgt gtcggccggg tggtgaaagc
      301 gatgcgcagc atcggcggcg aggtcgtcat caccgccgac cacggcaacg cagaatgcat
      361 ggaagaccac gccaatcgcc aaccgcatac ccaacatacc accaatctgg tgccgctgct
      421 gtatgtgggc cgcccggcgc agcttgcgcc aaccggcgca ctgtccgacg ttgcccctac
      481 cctgctgcat ttgatggggc tgccacagcc ggcggagatg acggggcata cgctggtcga
      541 ctccgcagac atgccagtcg cggctaatgc ccgataacat gcgggcgcac cagcgctgtg
      601 cgctcgcact gacattcgcc ctgtttttca cgccatttcc tgcggacgcc ggcaagcagg
      661 aggccgccag ggagtcgctg gagcaactgc aaaatcgcat cgagtcgctc aagaaagaga
      721 tggaaaagac cgagggcgca cacgccgaag ctgctgacgc cctcaagcag agcgagcagg
      781 ccatcagcga agctaaccgc aaactgtatg aactcaacca gcagcaccgc gaaaacctcg
      841 ccactttgca aacgctgcaa caacagaagt ccggtctgga gcagacgatc ggcgagcagc
      901 agcgcaggct cggcgaactg ctctatcgcc agtatctcaa tagccagcaa agctatttgc
      961 aaatcgtgct gacagcgcac gaccccgcga cgattgcacg tgacctctac tacctcggct
     1021 atgtcgcccg cgcccgcgcc gacctgattg cctcgctgcg cgagaacctc gggcaggttg
     1081 ccgtgctcaa cgaaaaaacc acctctacgc tcgatcagat cgccgaactc aagtccgaac
     1141 aggaaaacca gcgcaaggaa ctgcaaaccc ggcagaaggc acgcaagagc ctgctcaaca
     1201 agctgtccac acagatcaaa tcgcagcgca gcgagattac caaactcaag cgcgacgaga
     1261 agcgtctgtc caacctggtg gagcgactgg cgcgcatcgt tccgaaaacg cccaaagcca
     1321 agccggccag ccgcgagaaa ccccgcctga atgaagccgt gccaacaact gatccggaca
     1381 gcggcagctt tgccgccctc aagggcaagc tgcgcctgcc gatacgcggc gaaattgcca
     1441 accgcttcgg caccccgcgc gaggacagcg gcgtcttctg gaaaggtctg ttcatcagga
     1501 gcggcgaggg gacagaagta aaatcagtcg ccgggggacg cgtggtgttc gccgactggc
     1561 tgcgcggttt cggcaatctg ctgatcgtcg accacggcga tggctacatg agcctgtatg
     1621 gcaacaatca ggcactgctg aaaaaagccg gcgaggaagt cagcgccggc gatgcactgg
     1681 ctaccgttgg cagcagcggc ggcagcgccg agtccgggct ctatttcgaa ctgcgctatc
     1741 agagcaagcc gctcgatccg cttgcctgga gcgtgaccag gtaagcccca tggtggccgc
     1801 acagacggta tgattttcct gttcacggat ttcggcgcca gcgacctcta tgtcggccag
     1861 atgaaggcac gcctgctgac ggccaaccct gatgccagga tggtcgacct gttgcatcag
     1921 gcgccgaact tcgacgtcag agccggcgca cacttgctgg ccgcgctggc gccgcaattg
     1981 gcacatggcg tgaccctggc ggtagtggac gccggcgtcg gtagcaaacg tcgcccggta
     2041 gcggtgcagg ccgacaatgg ctggtatgtc ggccccgaca acggcctgct gtcagtactg
     2101 gtagcgcgcg cacagaccag ccgttgctgg gagatcatct ggcagccgcc gcatttgtcc
     2161 cactccttcc acggccgcga cctgttcgca cccattgccg ccatgctgga atccagcgcg
     2221 tggtggccgc atgcgctacg cgaggtctcg gggttgaaca tcgatcttgg cgcggccgac
     2281 ctggcggaag tgatctatac cgaccattac ggcaacgcca tgaccggcct gcgcagcggc
     2341 catctgccgc aggagactga aatcatcgtg gccggccaca ggattggcta tgcgcgggtt
     2401 ttcgcagaag caccgcaggg agcgccgttc tggtatgaga acagcatcgg gctggtggag
     2461 atcgccgtca atcgtggcag cgcagcccaa ctgcttgggg tcggccctgg cagcctggtc
     2521 agggtgattg catgaagtcc tgaacctcct gcacgccaaa cggccagcac agttcatcgc
     2581 cgctatccgg ccgtagcagc accggtatct tcgtcccgta gcgcgccagc aaggcatcgt
     2641 cctcgctaat ctcgacatcc cgccaggaga gtcccgcctc gcgcaggaca agctgcgcgt
     2701 cctcgcacag gtgacagttc tcggtgccgt aaaggttcag catggctttg caccaattca
     2761 ttgagggaaa ataaatttta gccgaaccgg tgcggcggcc acgaattttc ttaaaatagc
     2821 gctttgtcac cacgcgcgct tactgaagcc tgacagcatg gccgaaaacg aacaatccaa
     2881 ggaaagcctg caggaaatcc tgcaacaggt gattcacctg ctgaacaagc atcgactggt
     2941 ggaaggtctg gtgcacaagc aggatatgcc gcgccacgac ctggtcgaat cgctggtgca
     3001 caagcagaac ctgtccgagc tgcaacacaa gctggatgag ctgcacccgg ccgacgtcgc
     3061 ctacatcctc gaggcgctgc cgatcgagca gcgtctgctg gtatgggatc tggtcaagac
     3121 cgagcgcgac ggtgacatcc tgctggaagt ttccgatgcc gtccgccaga cgctgattgc
     3181 cgacatggac agcgacgagt tgctggccgc ggccgagcag ctcgacaccg acgagatcgc
     3241 cgacctcgcg cccgacctgc cgcgcgacgt gctgcaagaa cttctggtca gcctggacgg
     3301 ccagaaccgc gagcgcctgc aatcggcatt gtcctacccg gaagacacgg tcggcgcact
     3361 gatggatttc gatatcgtca ccatccgtga tgacatcagc ctggaagtcg cgttgcgcta
     3421 cctgcgccgc ctcggcaagc tgccggatca tactgacaaa ctgttcgttg ttgacagaga
     3481 cgatatcctg cgcggcgtac tgccgctgaa acggctggtg gtatatgatc ccgaggccag
     3541 cgtggctgca gtgatggcag acgacgcggt gatcttccac cctgaggagc aggccagcga
     3601 tgcggcacag gccttccgcc gctatgacct ggtgacggcg ccggtggtag atagcaataa
     3661 caagctggtc ggccgcctga ccgtggatgc ggtagtggac ttcattcagg acgaatcgga
     3721 gagcgaaatg ctgtccatgg ccggcctgcg tgaagaggag gacctgttct cttcggtatg
     3781 gaagtcggtg cagaatcgct ggacctggct ggcaatcaac ctgatgacgg cattcatcgc
     3841 ttcacgcgtg atcggggtgt tcgagggctc gatcgagaag atcgtcgcct tggctgcctt
     3901 gatgccgatc gtcgccggta tcggcggaaa ttccggcaac cagaccacca ccatgatcgt
     3961 gcgcggcctc gctttgggac agatcagtgc actcaacatg caaaaactgc tgttgaagga
     4021 gcttggggtt gcgctgctca acgggctggt atggggcagc gtacttgggg ttgccgcatt
     4081 cctgctctat cacaatctgc cgcttgggct ggtcatgacc ggtgcgatga ccttgaatct
     4141 tatgctggcc gcactgatgg gggtgggtat tccgctggcc atgacccgtc taggacgcga
     4201 tccggcagta ggctccagcg tgctgattac ggcagtgact gacagcggcg ggttctttat
     4261 ctttcttggt ttggccacat tcttcctgct ctgaggcaat tgatgggatc cgaaataatc
     4321 aacatgtgga cagaactggc ggccgacctg cgcacgccag cggtatacgg gcaactgatc
     4381 gtcctcgtcc tcagcctttc cctggcatgg ctgctgaacc gcattctgca tggccagatc
     4441 atgcacaaag cgcctgaatc gtggaaagtc ggcatcggcg gcgccaagcg gctgttgttc
     4501 ccgctgagct cgctgctttt cgtccacctt ggcatgctgg tactgcgcca cggcggtaat
     4561 cacaccagca tgctcaagct ggcatcctcg ctgctggtcg ccatggcggt gatccggttg
     4621 acggtctata cgctgcgcta cgtgtttgcg ccaagcaact ggctgcgtac cgcggagagc
     4681 gccattgtcg gtacggtatg gggactggtg gcgttgcacc tggtaggctt cctgccggaa
     4741 ctgttgcggg cactggatga aatcagcttc accatgggca agcacaccat ctcgttatgg
     4801 ctgatcctgc aggccgccgc catcgtcgtg gtgaccatca tccttgcgct gtcggccagc
     4861 cgcttcctcg aaaacaagct gatgcgcgcc gaacagatcg acatgaacat gcgcgtggtg
     4921 ctgagcaagc tgctgcgtgt agtactgacc ctggtgggtg tgctcaccgc gctttcggca
     4981 gtcggcttcg acatcacgct gctatccgta ttcggtggag cgctaggcgt cggcctggga
     5041 ctcggcctgc agaaaatcgc cagtaactat gtcagcggtt tcatcatcct gctggatcag
     5101 tcggtacaca tgggcgacgt catgaccatc aacgaccatt acggtgtggt gtaccagata
     5161 cgttcacgct acctggtatt gcgcaagctg gatggcaccg aagtcatcat ccccaacgag
     5221 acgctgattg cctccgcagt cattaatcat tctctttccg accgcaaggc acgcatcctg
     5281 atgccggtgc agatcagcta cgacagtccg ctggaactgg ccatgcaggc aatgcgcgag
     5341 gtagcgctgc ggcagccgcg ggtattaaag gacccagggc cggaagtcct ggtcaagggc
     5401 ttcggcgaga gcggcatcga cctcaacctg tccatctgga tcgaggatcc ggaagaggga
     5461 tcaggcagcc tgcaatccgc gatatacctg ggcatctggc gcgaattcca gcaacagggc
     5521 gttgccggcc cc
//
LOCUS       DAOMFA010000107         5521 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_1918_length_5521_cov_4.794914, whole genome shotgun sequence.
ACCESSION   DAOMFA010000107 DAOMFA010000000
VERSION     DAOMFA010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 5521)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5521)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5521
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1918_length_5521_cov_4.794914"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..249
                     /locus_tag="PKW44_06285"
     CDS             <1..249
                     /locus_tag="PKW44_06285"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020184049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HQR51227.1"
                     /translation="ARMVKPFSDAVVGMKKGEYTNTPVQTQYGWHVIKLVDVRNTQPP
                     AYEKVKDGIQKELQQRQIEKMVSDLRAKAKIVDNSSTK"
     gene            383..610
                     /locus_tag="PKW44_06290"
     CDS             383..610
                     /locus_tag="PKW44_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018292809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HQR51228.1"
                     /translation="MKQPSSLASLQPGQHAVIRAIHAEEGLHHRLTALGFRIGKRIEL
                     VRRACFSGPLHVRIGSTDIILRQSEAHRIQI"
     gene            607..2394
                     /gene="feoB"
                     /locus_tag="PKW44_06295"
     CDS             607..2394
                     /gene="feoB"
                     /locus_tag="PKW44_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HQR51229.1"
                     /translation="MKRIALLGMPNTGKSTLFNRLSGASARVGNWPGITVDLMSAKIL
                     LGGHIAELVDLPGIYDLHGFSDDEQVVRHFLENNHVDLVLVVLNTCQIDRQLSLALQL
                     RGLGLPTLVLLNMADEAKRGGISVDLALMQQHLQLPVALLSAKYGDGCPQALALAAQT
                     LEAKPAPMPDALREQLQPDDRIEQEMNAILQHAVQLPTQFTDQTTTRLDHVLLHPWLG
                     LPLFFGALYLLFQFIFTAGKPLQDGVAWLLGYFRGGVLEPLLAGAPTWLHGLLLDGIY
                     NGVGTVAAFLPIIILFFLVMTLVEDSGYLSRAAFLMDAMMAKMGLDGRGFVMLLMGFG
                     CNVPALMGTRVMRSRPLRLLTMLVIPFSLCSARLQVFVFMTAALFTPQQAPLVLFALY
                     LTSFAATFASALLFKGRFTNSEPFILELPPYRLPTSHQILMRGWHETSQFLRRASKFI
                     IIGVVLVWALTHYPSDVPPASAYTFAGMIGNALAPALNPIGIDGQLAIALIFGFVAKE
                     IVIGSLAVIYGLQGDALIQVVAQKLDWVQAMSFMLFTLIYTPCLSTIATLRSESRSNG
                     FMWLSIGWSLLLAWSVSFIFYQTARGLGF"
     gene            2478..3053
                     /locus_tag="PKW44_06300"
     CDS             2478..3053
                     /locus_tag="PKW44_06300"
                     /inference="COORDINATES: protein motif:HMM:NF024901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HQR51230.1"
                     /translation="MKAQLLALVLVTAHATTTAAQTTVYESRDAQGNTVFSDKPSPGA
                     KAIELPQPNVIQTDLLPPPPAQAAPVSGAGKKASIDADATPVYSSLAIASPGDQDSIY
                     TNTGAFDVTLKIDPDLRTNQGDFIQLKLDGTLVPQQFSAPDISISSADWAASATDNTA
                     HTLQAAILDKQGKILIESAPVTFYAHRHSIL"
     gene            complement(3084..3437)
                     /locus_tag="PKW44_06305"
     CDS             complement(3084..3437)
                     /locus_tag="PKW44_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019919243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HQR51231.1"
                     /translation="MGSIPAWVQAAGLLILSNVFMTFAWYAHLKNLNDRPWWIAALVS
                     WGIALFEYQFQVPGNRIGYTELSLAQLKIMQEVIALAVFVPFAVLYMQQPLKLDYLWA
                     GLCLMGAIYFIFRAG"
     gene            complement(3460..3798)
                     /locus_tag="PKW44_06310"
     CDS             complement(3460..3798)
                     /locus_tag="PKW44_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018229815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HQR51232.1"
                     /translation="MKKIEAIIKPFKLDEVREALSEIGVNGLTVTEVKGFGRQKGHTE
                     LYRGAEYVVDFLPKIKLELVVAENLVEAAIEAIVKAAHTGKIGDGKIFVSSVEQVIRI
                     RTGETGEAAV"
     gene            complement(3812..5416)
                     /locus_tag="PKW44_06315"
     CDS             complement(3812..5416)
                     /locus_tag="PKW44_06315"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HQR51233.1"
                     /translation="MKLAIAQINSSVGDLDGNAEKIAEYARRAKADSASLLLTPELAL
                     AGYPPEDLLLRDDFYLACHRALQRLAAAADGITIVVGHPAERGGEHYNAASVLRDGKI
                     VATYFKHKLPNYGVFDEVRYFVPGDAPLVFEHEGIRIGLLICADVWEPGPAVQARDAG
                     AQILLALNASPYHLEKQSTRYEVLRARVAETGLPVVYANLVGGQDELVFDGASFVLNA
                     DGSLTHQFAAFEEALLMVEFEAAHPLSGKVMADSGLEASVYQALCLGVHDYIVKNGFP
                     GVLLGLSGGIDSALVLAIAVDALGADKVRAVMMPSRFTADISQTDAARVARNLGVDYT
                     EIPIERLFESFLDALAAEFKGQMFDLTEENLQARIRGTLLMAISNKFGAIVLTTGNKS
                     EMSVGYSTLYGDMAGGFAVLKDVPKTLVYRLARYRNTISPVIPERIITRAPSAELRPD
                     QTDQDSLPPYETLDGIMEAYVEHDKSPVQIMAMGYDREDVRRVVTLIDRNEYKRRQSP
                     VGVRITQRGFGKDRRYPITSKYRFEF"
BASE COUNT         1136 a         1788 c         1516 g         1081 t
ORIGIN      
        1 gcacgcatgg tcaagccttt tagtgacgca gtggttggca tgaaaaaagg cgagtacacc
       61 aacactccag tacaaaccca gtatggctgg catgtcatca agctggtcga tgtccgcaac
      121 acgcagcctc ccgcttatga aaaggtcaaa gatggcatcc agaaggagct gcagcagcgt
      181 cagatagaga aaatggtctc cgaccttcgc gccaaggcca aaatcgtgga taacagcagc
      241 accaaataat ttatttccag cccatcagcc gggcaacact aactaaaaag cctgtatcaa
      301 acccgataca ggctttttta cttacaacag aacatgcttg tgtaataata agaaccgtta
      361 tcatttgaaa agccggtcac tcatgaagca gccctctagt ctcgccagcc tgcaacccgg
      421 tcagcatgcc gtgatccgcg ctatccatgc cgaagaaggg ctgcaccacc gtcttaccgc
      481 ccttggtttc cgcatcggca agcgcatcga actggtacgc cgcgcctgtt tttccggccc
      541 actgcacgta cgcatcggca gcaccgacat catcctgcgc cagtccgaag cccaccgtat
      601 ccagatatga aacgtatcgc cctgctgggc atgcccaaca cgggcaagtc caccctgttc
      661 aaccggcttt ccggcgcctc ggcgcgcgtc ggcaactggc cgggcatcac ggtagatctg
      721 atgagcgcaa aaatcctgct cggcggccac atcgccgagc tggtggacct gcccgggatt
      781 tacgacctgc acggcttttc cgacgacgaa caggtggtgc gccactttct cgaaaacaac
      841 catgtcgatc tggtactggt cgtgctcaac acctgccaga ttgatcgcca actctcgctt
      901 gcattgcagc tacgcggcct cggcctgccc acgctggtgt tgctcaacat ggcggacgaa
      961 gccaaacgcg gcggtatcag tgttgacctg gcgctgatgc agcagcatct gcaactgccg
     1021 gtggcgctgc tcagcgccaa atacggcgac ggctgcccgc aggcgctggc actcgccgcg
     1081 caaaccctgg aggccaagcc cgcccccatg ccggatgcct tgcgcgagca actgcaaccg
     1141 gacgaccgca tcgagcagga aatgaacgcc atcctgcagc atgccgtaca actgccgacg
     1201 cagttcaccg accagaccac gacgcgcctg gaccacgtgc tgctacaccc ctggctgggg
     1261 ctgccgctgt tttttggcgc actttatctg ctattccagt tcatcttcac cgccggcaag
     1321 ccattgcagg acggcgtcgc ctggctgctc ggctatttcc gcggcggcgt gctggagccg
     1381 ctgctggccg gcgccccgac ctggctgcac ggcctgctgc tggacggcat ctacaacggc
     1441 gtcggcacgg tagcggcatt ccttcccatc atcattctgt tcttcctggt catgacgctg
     1501 gtggaagaca gcggctacct ctcgcgcgcc gccttcctga tggacgcgat gatggcgaaa
     1561 atgggcctgg acgggcgcgg cttcgtcatg ctgctgatgg gcttcggctg caacgtgccg
     1621 gcgctgatgg gtacgcgcgt catgcgttcg cgtcccttgc gtctgctcac catgctggtg
     1681 attcccttct cgctgtgctc ggcacggcta caggtgttcg tgttcatgac cgccgccctg
     1741 tttaccccgc agcaggcgcc actggtattg ttcgcgctct acctcaccag ttttgccgcc
     1801 acctttgcca gcgccctgct attcaagggg cgcttcacca atagcgagcc gttcatcctg
     1861 gaattgccgc cctaccgcct gccgacctcc caccagattc tgatgcgcgg ctggcacgag
     1921 accagtcagt tcctgaggcg ggcaagcaag ttcatcatca tcggcgtggt gctggtatgg
     1981 gcgctgaccc actaccccag cgacgtaccc cccgccagcg cctacacttt cgccggcatg
     2041 atcggcaacg ctctggcgcc ggcactcaat cccatcggca ttgacggtca acttgccatc
     2101 gcactcattt tcggctttgt cgccaaggaa atcgtcatcg gctcgcttgc cgtgatctac
     2161 ggcctgcagg gcgatgccct gatacaggtc gtggcgcaga aactggactg ggtacaagcc
     2221 atgagcttca tgctgttcac gctcatctac acgccctgcc tgtccaccat cgccaccctg
     2281 cgcagcgagt ccagaagcaa cggcttcatg tggctttcga tcgggtggtc gctgctgctg
     2341 gcatggagcg tcagcttcat cttttaccag accgctcgcg gtcttggctt ctagctcgga
     2401 tatttctggc tgctcgacac catgctagac tgcatgcgtg tcaacagcac gcatgcactg
     2461 atcatggaga atgcaggatg aaagcgcaat tactcgccct agttctggtc acggcgcacg
     2521 ccacaaccac tgcggcacag accaccgttt acgaatccag ggacgcgcaa gggaataccg
     2581 tattttcaga caagccttcc cccggtgcca aggcgatcga actgccgcag cccaacgtga
     2641 ttcagactga tctgctacct ccgccacctg cgcaagcagc gccagtttcc ggcgctggca
     2701 agaaagcgag catcgatgcc gatgcgacac ctgtttacag ttcgcttgca atagcttccc
     2761 ctggcgatca ggattcgatc tacaccaaca ccggcgcgtt cgatgtgacg ctgaaaattg
     2821 accctgattt gcgaaccaac cagggcgact tcatccagct gaaactagac ggaacgctgg
     2881 taccgcaaca attcagcgcc cccgacatca gcatcagctc agcggactgg gcagccagcg
     2941 cgaccgacaa caccgcgcac acgctgcaag ccgccatcct cgacaagcag ggcaagatac
     3001 tgatcgagtc ggcgccggtc acgttctacg cacaccggca ttccatatta tgaggatatt
     3061 ccggcatcgg cagggcggga ccttcacccc gcgcggaaga tgaagtaaat cgcccccatc
     3121 aggcacagcc ccgcccataa ataatcgagc ttgagcggct gctgcatgta gagcacggca
     3181 aaaggaacga acaccgccaa tgcgatcact tcctgcatga tcttgagctg tgcgagcgac
     3241 agttccgtat agccgatgcg attgcccggc acctggaact ggtactcgaa cagggcgatg
     3301 ccccagctca ccagcgcggc gatccaccag ggccggtcgt tgaggttttt gagatgggca
     3361 taccaggcga acgtcatgaa tacgttcgac aggatgagca ggcctgccgc ctgcacccag
     3421 gccgggatag agcccattgc cgcttattcc ggccggggat cagactgccg cttcacccgt
     3481 ttctccggta cggatacgga tcacctgctc caccgaactg acgaaaatct tgccatcacc
     3541 gatcttgccg gtgtgcgcgg ccttgacgat ggcttcaatg gcggcctcca ccaggttttc
     3601 cgccacgacc agttccagct tgatcttggg caggaagtcc accacgtact cggcgccgcg
     3661 atagagctcg gtatggccct tctggcggcc gaagcccttg acttcggtca cggtcaggcc
     3721 gttgacgccg atttccgaca gtgcttcgcg cacttcgtcg agtttgaacg gcttgatgat
     3781 ggcttcgatc tttttcatgg tttttcctcg gtcagaattc aaaacgatac ttggatgtta
     3841 tcggataacg cctgtccttg ccaaacccgc gctgagtgat gcgcacgccg acaggagact
     3901 ggcggcgctt gtactcgtta cgatcaatca gcgtcaccac gcgccgcacg tcctcacggt
     3961 catagcccat ggccatgatc tgcaccgggc ttttgtcgtg ctcgacatag gcttccatga
     4021 tgccgtccag ggtctcgtaa ggcggcagcg aatcctggtc agtctggtcg ggccgcaact
     4081 cggccgacgg cgcacgggtg atgatacgct ccgggatgac cggggaaatg gtattgcggt
     4141 agcgcgccag acgatagacc agcgtcttgg ggacgtcctt cagcactgca aagccgccgg
     4201 ccatgtcacc gtaaagcgtg gaatagccca cgctcatctc gctcttgttg cctgtcgtca
     4261 acacgatagc gccgaacttg ttggaaatcg ccatcagcag ggtaccgcga atccgtgcct
     4321 gcagattctc ttcggtaaga tcgaacatct ggcctttgaa ctcagcggcc agcgcgtcca
     4381 gaaaactctc gaacagccgc tcgataggga tttcggtata gtccacgcca aggtttctgg
     4441 ccactcgcgc agcatcggtc tggctgatgt cggcagtgaa gcgggaaggc atcatcacgg
     4501 cacgtacctt gtccgccccc agcgcatcga ccgcgatcgc caacactagt gccgaatcga
     4561 tgccgcccga caatcccagc agcacccccg gaaagccgtt ttttacgatg tagtcatgca
     4621 cacccaggca gagtgcctga tacaccgaag cctccaggcc agaatcggcc atcactttgc
     4681 ccgacaacgg atgcgcggcc tcgaactcga ccatcagcaa ggcctcctcg aacgcagcaa
     4741 actggtgcgt caagctgcca tccgcgttca gcacgaaaga ggcgccgtcg aacaccagtt
     4801 cgtcctggcc accgaccaga ttggcgtaga ccaccggcag tccggtttcg gcaacccgtg
     4861 cacgcaacac ctcgtagcgc gtcgactgct tctccaggtg gtaaggcgag gcgttgagcg
     4921 ccagcaggat ttgagcgccc gcatcgcgcg cctgcaccgc cgggccgggc tcccagacgt
     4981 cggcgcagat cagcaggccg atgcggatac cctcgtgctc gaacaccagc ggtgcatcgc
     5041 caggaacgaa atagcgcact tcatcgaaca cgccgtagtt gggaagcttg tgcttgaaat
     5101 aggtggcgac gatcttgcca tcacgcagca ccgaagccgc attgtagtgc tcgccacccc
     5161 gttccgccgg atgccccacc acaatagtga tgccgtcggc ggccgccgcc aggcgctgca
     5221 gtgcgcgatg gcaggcgagg taaaagtcgt cccgcagcag caggtcttcc ggcggatagc
     5281 cggccagcgc cagctccggc gtcagcagca acgatgcact atccgctttg gcacgccgcg
     5341 cgtattcggc aatcttttcg gcattgccgt caaggtcgcc gaccgagctg ttgatttgcg
     5401 cgatggccag tttcacacgg gcaccccatt ccatgccaaa aacttggtag aagcgccgcc
     5461 ttcggagcga caagaatcgg aaaatcgagt cgaagcggcg ctcctacgag agatcatggt
     5521 t
//
LOCUS       DAOMFA010000108         5413 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_1978_length_5413_cov_6.183837, whole genome shotgun sequence.
ACCESSION   DAOMFA010000108 DAOMFA010000000
VERSION     DAOMFA010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 5413)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5413)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5413
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1978_length_5413_cov_6.183837"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(91..1392)
                     /gene="hisD"
                     /locus_tag="PKW44_06320"
     CDS             complement(91..1392)
                     /gene="hisD"
                     /locus_tag="PKW44_06320"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HQR51234.1"
                     /translation="MLNIRRYNTKDSAFEASLHQLLAFEGAQDDKIDAVVAGILADVK
                     KRGDAAVLDYTQRFDRLGADRMSDLELPQVELGQALDSLPADQCDALKLAADRVRAYH
                     EKQLMPSWSYTEADGTMLGQQVTALDRVGLYVPGGKAAYPSSVLMNAIPAKVAGVQEL
                     IMVVPTPGGEKNQLVLAAAAVCGVDRVFCIGGAQAVGALAYGTQTVPQVDKIVGPGNA
                     YVAAAKRRVFGVVGIDMVAGPSEILVISDGNSDPDWTAMDLFSQAEHDELAQAILLSP
                     NGEFLDRVQASINRLLLEMPRKDIITASLEGRGALIQVRDLDEAAKIANYISPEHLEL
                     SVNDPVTLTKKIRHAGAIFMGRQTCEALGDYCAGPNHVLPTSRTARFSSPLGVYDFQK
                     RSSLIMVSEAGAQTLGRIASTLAHGEGLQAHARSAEYRLKG"
     gene            complement(1394..2038)
                     /gene="hisG"
                     /locus_tag="PKW44_06325"
     CDS             complement(1394..2038)
                     /gene="hisG"
                     /locus_tag="PKW44_06325"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HQR51235.1"
                     /translation="MSQITIALSKGRIFEETLPLLAAAGIEPAEDPESSRKLIIGTNR
                     DDVRLIIVRASDVPTYVQYGAADLGIAGKDVLVEHGGSGLYQPLDLQIAKCKMMVAVR
                     QDFDYAGSIRSGARLKVATKYVKIAREHFAEKGMHVDLIKLYGSMELAPLVGLADAIV
                     DLVSTGGTLRANNLTAVEEIMPISSRLVVNQASLKLKRSQIQPIIDAFSQAVGE"
     gene            complement(2039..3301)
                     /gene="murA"
                     /locus_tag="PKW44_06330"
     CDS             complement(2039..3301)
                     /gene="murA"
                     /locus_tag="PKW44_06330"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HQR51236.1"
                     /translation="MDKLVITGGQRLQGEVRVSGAKNAALPILCAALLTKEPLTLTNM
                     PALHDIQTMLKLLAQMGVKVAQDGDTVTLDASGLDNPAAPYEMVKTMRASVLVLGPLM
                     ARCGEAHVSLPGGCAIGARPVDQHIKGLQSMGAEIKVEQGYVNARVGKLKGARLFTDM
                     VTVTGTENLMMAACLADGETVIENAAREPEVVDLAQCLVSMGAQISGAGTDVIRIRGV
                     ERLHGATHRIMPDRIETGTYLCAAAITGGQVRLTGTSMGYLDAVVDKLMDAGCEITGE
                     KSPQFEAITLRAPKKLNAVSIRTAPYPAFPTDMQAQFMTINTVAEGAAVIRETIFENR
                     FMHAVELQRLGADIKIDGNTAVVRGVARLDGATVMATDLRASAGLVLAGLVAEGETTI
                     ERIYHLDRGYEHIEQKLSQLGAIIHRIN"
     gene            complement(3303..3614)
                     /gene="grxD"
                     /locus_tag="PKW44_06335"
     CDS             complement(3303..3614)
                     /gene="grxD"
                     /locus_tag="PKW44_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HQR51237.1"
                     /translation="MDVQERIRTQVTRNPVVLYMKGSPKFPQCGFSGVAAQILQACGV
                     KDYVAVDVLQDPDIREGIKTYANWPTIPQLYIKGEFVGGADIMREMYQSGELKKLLEA
                     A"
     gene            complement(3617..3862)
                     /locus_tag="PKW44_06340"
     CDS             complement(3617..3862)
                     /locus_tag="PKW44_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA/IbaG family iron-sulfur metabolism protein"
                     /protein_id="HQR51238.1"
                     /translation="MPSADQVRAYIMQGLPCEYVAVQGNDGQHFEAVIVSPQFVGKSK
                     VQQHQLVYQSLGERMRQEIHALSMRTFTPEIWASLEK"
     gene            complement(3865..4620)
                     /locus_tag="PKW44_06345"
     CDS             complement(3865..4620)
                     /locus_tag="PKW44_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009852951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQR51239.1"
                     /translation="MIGMWTLFKKEILRFWKVSFQTVAAPVITALLYLLIFSHVLEGR
                     VQVYSGVSYTAFLIPGLVMMSVLQNAFANSSSSLIQAKVMGNIVFLLLTPLSYREFFV
                     AFLAASVIRGLVVGLGVYLVALLFIPLPLAHPLAALAFAIAGSAMLGALGIIAGIWAD
                     KFDQMAAFQNFIIMPLSFLSGVFYSIHSLPPFWQKVSHLNPFFYMIDGFRYGFFGQGD
                     IDPGVSLAVVAISFFALSWATLRLLKSGYKLRG"
     gene            complement(4617..>5413)
                     /locus_tag="PKW44_06350"
     CDS             complement(4617..>5413)
                     /locus_tag="PKW44_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQR51240.1"
                     /translation="LGPNGAGKSTLINLLAGLLRPTSGKLSVMGHDVVNDYQLARKAL
                     GVVPQELVFDPFFNVREMLRFQAGYFGRGKENYAWIDEVIEGLGLLGQAGTNMRRLSG
                     GMKRRALIAQALAHKPPVIVLDEPTAGVDVELRQVLWEFIKQLNRAGHTIILTTHYLE
                     EAEALCSRVAMLKQGQIVALDDTRTLLQRYAGKSLRLRLDALHIPAGLRPLLRSEEGG
                     YCTLALHEVGQLEGVLAQLREAGIKVEDMQLIEADLEDVFMEIVRQ"
BASE COUNT         1087 a         1738 c         1536 g         1052 t
ORIGIN      
        1 tgagcttgat caggttggcg atcttcggct gctctcccgg cacgtagggg gtgagccgat
       61 tgacgacctc gctccaatag ttgctcaaga tcagcctttc agccggtact cggcagaacg
      121 tgcgtgcgcc tgcaggccct cgccatgcgc cagcgtggaa gcgattctgc ccagcgtctg
      181 cgcaccagct tccgacacca tgataaggct ggatcgcttc tggaagtcgt acacccccag
      241 cggcgaggaa aagcgcgccg tacgcgaagt cggcagcacg tggttggggc cggcacaata
      301 gtcgcccagc gcctcgcagg tctggcggcc catgaaaatg gcaccggcat gcctgatttt
      361 tttggtcagc gtaaccgggt cgttcacgga aagctccagg tgctccggcg aaatatagtt
      421 ggcgatcttg gcagcctcgt ccaggtcgcg cacctggatc agcgcgccac ggccttccag
      481 ggaggcggtg ataatatcct tgcgcggcat ttccagcagc agacggttga tcgatgcctg
      541 cacgcgatcg aggaattcgc cgttgggaga cagcaggatg gcttgcgcca gttcatcgtg
      601 ctcggcctgc gagaacaggt ccatcgccgt ccagtcgggg tcgctgttgc cgtccgaaat
      661 taccaggatt tccgacgggc cggccaccat gtcgatgccc accacgccga acacgcggcg
      721 cttggccgcg gccacatagg cgttgccagg cccgacgatc ttgtctacct gcggtaccgt
      781 ttgcgtaccg tacgccaagg ctccgacggc ttgcgcgccg ccaatgcaga acacgcggtc
      841 gaccccacac accgcggcag cggccagcac caactggttc ttttctccgc ccggcgtagg
      901 caccaccatg atcagctcct gcacccccgc caccttggcc ggaatcgcgt tcatcagtac
      961 cgacgacgga taagcggcct taccgcccgg cacgtagagg ccgacgcggt ccagtgccgt
     1021 cacctgctgg ccgagcatgg tgccatcggc ttcggtgtag ctccaggacg gcatcaactg
     1081 cttctcgtgg taggcgcgca cgcggtcggc ggccagttta agcgcatcgc actggtcggc
     1141 cggcaggctg tccagcgctt gccccaattc tacctgcggc agttccaggt ctgacatccg
     1201 gtctgcgccg aggcggtcga agcgctgcgt gtagtccagc accgcagcat cgcctcgttt
     1261 tttcacgtcg gccagtatcc cggccaccac ggcatctatc ttgtcgtctt gcgcgccctc
     1321 gaaagccaat aactgatgca gcgaagcctc gaaggcggaa tccttggtgt tgtagcgtct
     1381 gatattaagc atgttattcc ccgaccgctt gtgaaaaggc atcgataatc ggctggatct
     1441 ggctacgctt gagcttgagc gatgcctgat tgaccaccag ccgcgaactg atgggcatga
     1501 tttcctcgac cgcggtcagg ttgttggcgc gcaacgtgcc gccggtactg accaggtcga
     1561 cgatggcatc tgccagcccg accagcgggg caagttccat cgagccatag agcttgataa
     1621 gatcgacatg catacccttt tcggcgaagt gctcgcgcgc aatcttcacg tacttggtcg
     1681 ccaccttgag gcgcgcgcca ctgcgaatcg aacccgcata gtcgaaatcc tgacgcaccg
     1741 ccaccatcat tttgcacttg gcgatctgca ggtccaatgg ctggtacagg ccgctaccgc
     1801 catgctcgac cagcacgtcc ttgccggcga tgccgaggtc ggcggcgcca tactgcacat
     1861 aggtcggcac atcagaggcg cgcacgatga tcaatctgac atcgtcgcga ttggtgccaa
     1921 taatcagctt gcgcgaggat tccggatcct cggccggctc gatgcccgct gccgccagca
     1981 agggcagggt ttcctcgaag atgcgtccct tggacagggc aatcgtaatt tggctcattt
     2041 agtttatccg gtgaataatc gcacccagtt gcgacaactt ctgttcgata tgttcatagc
     2101 cacgatcgag atggtaaatg cgttctatcg tggtctcgcc ctctgctacc aacccggcca
     2161 gcaccaaccc tgccgacgcg cgaaggtcgg tcgccatcac cgtggcgccg tccaatctgg
     2221 ccacgccacg caccacggcg gtattgccgt cgatcttgat gtccgcaccc aaacgctgta
     2281 actcgacggc atgcatgaag cggttctcga aaatggtttc gcggatcact gcggcaccct
     2341 cggccacggt attgatggtc atgaactgcg cctgcatgtc ggtgggaaag gccggatacg
     2401 gcgcggtacg gatggacacc gcattgagct ttttcggcgc ccgcagggta atcgcctcga
     2461 actgcggcga tttttcacct gtgatttcgc agcccgcgtc catcagcttg tccaccacgg
     2521 catccagata acccatggag gtgccggtca agcgcacctg accgccggtg atggcggcgg
     2581 cgcagaggta ggtgccagtc tcgatacgat caggcatgat acggtgggtg gcgccgtgca
     2641 ggcgctccac gccgcggatg cggatgacgt cggtaccggc gccactgatc tgcgcgccca
     2701 tggacaccag gcactgcgcc agatccacca cttccggctc gcgcgcggca ttctcgatca
     2761 ctgtttcgcc atccgccagg caggcggcca tcatcaggtt ctcagtgccg gtcaccgtca
     2821 ccatatcggt aaacaggcgc gcgcccttga gcttgcccac ccgggcattg acgtagccct
     2881 gctcgacctt gatctcagcg cccatggact gcaggccctt gatgtgctgg tctaccggcc
     2941 gcgcaccgat ggcgcaaccg ccaggcaagc tgacatgggc ttcgccgcaa cgcgccatca
     3001 gcggccccag caccagcacc gatgcacgca tggtcttgac catttcatac ggtgcggcag
     3061 ggttgtcgag accggaagca tccagcgtaa cggtgtcgcc gtcctgtgcc accttgacgc
     3121 ccatctgtgc cagcagcttg agcatggtct ggatgtcgtg aagtgccggc atgttggtca
     3181 gcgttagcgg ttccttagta agcagcgccg cgcacaggat cggcagcgcc gcgtttttgg
     3241 caccggaaac ccgcacttcg ccctgcagcc gctggccgcc ggtgatgact aatttgtcca
     3301 aatcaggccg cctctagcaa tttttttagt tcgccgctct ggtacatctc acgcatgatg
     3361 tcggcgccgc cgacgaactc gcccttgatg tacaactgcg ggatggtcgg ccaattggcg
     3421 taagtcttga tgccctcgcg aatgtcggga tcttgcagca catccacagc aacataatcc
     3481 ttcacgccgc aggcctgcag gatctgcgca gccacgcctg agaaaccgca ttgcggaaac
     3541 ttggggctgc ccttcatgta aagcaccacc ggattccgtg tcacctgcgt gcgtattctt
     3601 tcctgtacgt ccattttcac ttctccagac ttgcccaaat ttcgggcgta aatgtgcgca
     3661 tcgaaagcgc atgaatttcc tgccgcatgc gctcgcctag tgactgatac accagttgat
     3721 gttgttgcac cttgctcttg ccgacgaatt gcgggctgac gatcaccgcc tcgaaatgct
     3781 ggccgtcgtt gccctgcacc gccacgtatt cgcaaggcaa tccctgcatg atataagccc
     3841 tgacttgatc ggcactcggc atgatcagcc tctcagcttg taaccgctct tgagcaatcg
     3901 caaagtcgcc catgataagg cgaaaaagct gatggcgaca acggcgaggc taacaccggg
     3961 atcaatatca ccctgaccga agaagccgta gcggaaaccg tctatcatgt agaaaaacgg
     4021 gttgaggtgt gacaccttct gccagaacgg tggcaacgag tggatcgagt agaacacacc
     4081 tgacaggaag gacagcggca tgatgatgaa gttctggaac gccgccatct gatcgaactt
     4141 gtccgcccag atacctgcaa taatgccgag cgcacccagc atggcgctgc cggcaatggc
     4201 gaaagccagc gccgccagcg gatgcgccag cggcaacggg atgaacagca gcgcgaccag
     4261 atataccccc agccccacca ccagcccacg aatgacggaa gcggcaagga atgcgacgaa
     4321 aaactcgcgg taagacaagg gcgtcagcaa caggaatacg atattgccca tgaccttggc
     4381 ctgaatcagg ctggacgaac tgttggcaaa ggcgttctgc agtaccgaca tcatcaccag
     4441 gccggggatc aggaaggccg tgtagctgac gccggaatac acctgcaccc ggccttccag
     4501 cacgtgcgag aaaatcagca gatacagcag cgcggtgatc accggcgccg ccaccgtctg
     4561 gaagctcact ttccagaagc gcaggatttc cttcttgaac agcgtccaca tgccgatcat
     4621 tgccgcacga tctccataaa cacatcctcc aggtcggcct cgatcagctg catgtcttcg
     4681 accttgattc cggcctcacg cagctgcgcc aagacaccct ccaactgccc gacctcatgc
     4741 agcgccagcg tgcagtagcc gccctcttct gagcgcagca agggccgcaa tccggccgga
     4801 atgtgcaacg catccagccg gaggcgcaag ctcttgccag cataccgttg caataaagtc
     4861 cgggtatcgt caagcgccac gatctggccc tgcttcagca tcgcaacccg cgagcacagg
     4921 gcttcggcct cttccaggta atgggtggta aggatgatgg tgtggccagc gcggttgagc
     4981 tgcttgatga actcccacaa cacttggcgc agttccacat ctaccccggc ggtcggctcg
     5041 tccagcacga tcaccggcgg cttgtgcgcc agcgcctggg cgatcagcgc gcgccgcttc
     5101 atgcctcccg acaggcggcg catattcgtg ccagcctgac cgagcaggcc gagaccctcg
     5161 atcacctcgt cgatccatgc gtaattttcc ttgccgcgcc caaaataacc agcctgaaag
     5221 cgcaacatct cgcgcacatt gaaaaacggg tcaaacacca gctcctgcgg caccacgccg
     5281 agcgcttttc tggcgagctg gtagtcattc accacatcat gccccatcac gctcaacttg
     5341 ccactggtag gccgtagcag gccggcaagc agattgatga gggtggattt tccggcgccg
     5401 ttcggtccca gaa
//
LOCUS       DAOMFA010000109         5403 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_1985_length_5403_cov_4.994203, whole genome shotgun sequence.
ACCESSION   DAOMFA010000109 DAOMFA010000000
VERSION     DAOMFA010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 5403)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5403)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5403
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1985_length_5403_cov_4.994203"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1138
                     /gene="rlmD"
                     /locus_tag="PKW44_06355"
     CDS             <1..1138
                     /gene="rlmD"
                     /locus_tag="PKW44_06355"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HQR51241.1"
                     /translation="SPRRVTPQCPHFGVCGGCVLQHMELSAQVAAKQRLLEDNLWHLA
                     KLKPETMLPPIQGPAWGYRHKARMRARYVPGKGGVLVGFNEKAKSFVAVMQQCEILPP
                     HISALIVPLQELLARLSIKDRMPQIEVAVGEHVSVLVLRILEPLQAADEPILREFADA
                     YGVQIWLQSKGPETATPFHPLEAPQLSYSLPEYDLVYPFMPTEFTQVNPDINRVLVRR
                     AMGLLDPRPGERIADFFCGLGNFTLPIARKGATVLGMEGSAALVCRARENAERNGLTG
                     VTEFREADLFEKTADSLAALGRFDKWLVDPPRDGALELIKAIEDHSAPQRIVYVSCNP
                     ATLARDAGVLVQVKGYRMLAAGVINMFPHTAHVESIALFEKQPS"
     gene            1388..2794
                     /locus_tag="PKW44_06360"
     CDS             1388..2794
                     /locus_tag="PKW44_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HQR51242.1"
                     /translation="MGCARSIDRPEIHFAIAQAPLNLDPRYATDAASERVNRLVYRPL
                     VDFDNASRAVPALADWERRDSSHYRFTLRPGGRTFHDGSHLVATDVVATYRSLLAIKD
                     TPHAAEFANIAAVEAVDANTVDFTLHQPDEQFPARLIVGILPERLVTSGHDFSHHPVG
                     SGPFKLSEWNGTLKLERVADHQIIVLDEVKDPTVRVLKLLRGEADLLQGDLPPELVVY
                     LKRQGGVTVREIPGANYSYLGFNLQDPWLKNPLVRRAVTLAIDRDAIIRDAMVGGSRA
                     ATAILPPEHWAGNPSLKPHFYDPQQAKSLLQQAGVQLPLKLVYKTSTDAQRVRLATMM
                     QAQMREAGIEVEIRSLDWGTFFDDVKHGKFQLYGLTWVGIKTPEIYRLAFHSTSVPPA
                     GANRGRLQDALLDGLIEKRDWPGATQRIHELLPYVPLWYEGQFAAMRSSVQGYAPAPD
                     GNWDALAMITRQQTGNAK"
     gene            2791..3162
                     /locus_tag="PKW44_06365"
     CDS             2791..3162
                     /locus_tag="PKW44_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HQR51243.1"
                     /translation="MIKINRMMHTGLIIADLQRSRAFYEGLLGFTPNPNRPKFGFEGV
                     WYDIGANQVHLMVVPNPYTGVVHPEHGGRDIHIAFSVDDVEQVRVELEKAGIPYSMSK
                     SGRAALFCRDPDGNALEFSAV"
     gene            3162..3641
                     /locus_tag="PKW44_06370"
     CDS             3162..3641
                     /locus_tag="PKW44_06370"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019918451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HQR51244.1"
                     /translation="MRIEVFITRQSLRLLDDERLVLADYPVSTALNGAGEAKGSGCTP
                     CGRHVVRARIGAGRPEGTVFIGRRPTGEIWSPQLAAQFPERDWILTRILWLSGCEPGI
                     NRLGDVDTMQRYIYIHGTPDTEPMGVPRSHGCIRMRNADVIDLFDKVPAGTPVLISA"
     gene            3647..4558
                     /locus_tag="PKW44_06375"
     CDS             3647..4558
                     /locus_tag="PKW44_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQR51245.1"
                     /translation="MLKRLVGVLPVVFGVLLLTFLLIHLVPGDPVEVMLGESAAAADR
                     AQLRADLGLDRPLPVQFADYLVRLAHGDFGRSIHSRDEISSLLAERLPATAKLAISAL
                     LVALCIGLPLGIAAALNHRGWPDRVATLTSLTLSAMPHFWLGPLLMLVFALWLGWLPV
                     SGMEGWTSIVLPSLTLGSGLAAILTRMTRASLLEVLHEDYVRTARAKGLPERTVILRH
                     ALRAALLPVVTVVGLQLGFLLTGAVITETVFGWEGVGRLLVESIQKRDYPVSQACVLV
                     IALIYVVVNLLTDLAYARLDPRVRFRE"
     gene            4555..5340
                     /locus_tag="PKW44_06380"
     CDS             4555..5340
                     /locus_tag="PKW44_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQR51246.1"
                     /translation="MKSLPAAVLVFWVAVALMAPLLGMQPDAIHLERILSTPDMHAWL
                     GNDDLGRDILARLLAGAQVSLAVAAIVTGVTMVFGTTLGLVAGYFGGGVDRALMHVTD
                     VFLAFPGILLAIAFAAVLGPGVDNLVLALCLTSWVGYARLTRAQALALKGRQHVLAAR
                     SLGASTSRIMFRHMLPLLAAPLIVESTYKIASLIIAEASLSFLGLGIQPPHASWGSML
                     RDGVRYLLVAPHYVLAVGVSLMSLVLAVNLLGDTLRDSWDVKR"
BASE COUNT          924 a         1595 c         1723 g         1161 t
ORIGIN      
        1 gtcccccaga cgggtgacgc ctcagtgtcc tcatttcggc gtatgtggcg gctgcgtctt
       61 gcagcacatg gagctttcag cgcaggtcgc ggccaagcaa aggctactgg aggacaacct
      121 gtggcatctc gctaagctga aacccgaaac catgctgccg ccgatccagg ggccagcctg
      181 gggctaccgc cacaaggcac gtatgcgggc gcgctatgtg ccgggcaagg gcggggtgct
      241 ggtcggtttc aacgagaagg ccaagagctt tgtggcagtg atgcagcagt gcgaaatcct
      301 gccgccgcac atttccgccc tgatcgtgcc gttgcaggaa ttgttggcca ggctctcgat
      361 caaggaccgc atgccgcaga ttgaggtggc ggtgggcgag catgtctcgg tgctggtgct
      421 gcgcatcctg gaaccgttgc aggcagcgga cgagccgatc ctgcgcgagt tcgccgatgc
      481 ctatggcgtg cagatctggc tgcagagcaa aggcccggaa accgctacgc cgttccaccc
      541 gctggaggcg ccgcaactgt cgtactccct gccggagtac gacctggtct atcccttcat
      601 gcccaccgag ttcacccagg tcaaccccga tatcaaccgg gtattggtgc gccgtgccat
      661 ggggttgctc gatccgcggc cgggcgagcg catcgccgat ttcttctgtg gtctgggcaa
      721 cttcaccctg cccatcgccc gcaaaggcgc gacggtgctg ggcatggaag gctcggccgc
      781 gctggtgtgc cgcgcccggg aaaatgccga gcgcaacggg ttgacaggcg taacggaatt
      841 ccgcgaggcc gacctgttcg aaaagaccgc cgatagcctg gccgcgctcg ggcgtttcga
      901 caaatggctg gtcgatccgc cgcgcgacgg tgcgctggaa ctgatcaagg ccatcgaaga
      961 ccacagcgcg ccgcagcgta ttgtctatgt gtcatgcaat cccgccacgc tggcgcgcga
     1021 tgccggtgtg ctggtgcagg tcaagggtta tcgcatgctg gccgcagggg tcatcaatat
     1081 gttcccgcat accgcgcatg tggaatcgat cgcattgttc gaaaagcagc catcataaga
     1141 gccgcgctat tcatacgctg atgggatttt tgaaaggcag gtagatcatg gtgagacgga
     1201 atcagagact gttatgggaa gaaaagaatc gcaggggcga tgaccggcgg cgtcttagtc
     1261 gccgaaagtc ggacaagcta aaaatttttc tcaagtatct ggcggttgtc gtgctgacag
     1321 cggccttgct cagcatcatt cactagtgct gcgggcattt tttgttctgc tcgcttgcct
     1381 catcacgatg ggctgtgcgc ggtctatcga ccgtccggag atccattttg ccattgctca
     1441 ggcaccgctg aatctggatc cgcgctatgc gacggatgcc gcttccgagc gcgtcaaccg
     1501 cttggtctat cggccgctgg tggatttcga taacgcctcc cgggccgttc ccgcactggc
     1561 tgactgggag cgccgggatt cttcgcatta ccgcttcacg ctcaggccgg gaggccgcac
     1621 gtttcacgat ggcagccatt tggttgccac ggatgtcgtc gccacctacc gttctttgct
     1681 ggctatcaag gacacgccgc acgctgccga atttgccaac attgccgcgg tagaggcggt
     1741 ggatgccaac accgtcgatt tcaccctgca ccagccggac gagcaattcc ctgcgcggct
     1801 gatcgtcggc atcctgccag agcggctggt tacctcgggt cacgatttct cccatcatcc
     1861 ggtgggcagc gggccgttca aactctcgga atggaacgga actctcaagc tggaacgtgt
     1921 tgccgaccac cagattattg ttctggatga ggtgaaagat ccgaccgtgc gcgtgctcaa
     1981 gttgctgcgc ggcgaggccg atctgctgca aggcgatttg ccgcccgaac tggtggtata
     2041 tctcaagcgc caggggggag tgacggtacg tgaaatcccc ggcgccaact attcctatct
     2101 gggcttcaac ctgcaggacc cgtggctcaa gaatccgctg gttcgccgtg ccgtcacgct
     2161 ggcaatcgac cgtgatgcca tcatccgcga tgcgatggtc ggcgggagcc gtgccgccac
     2221 tgccatcctg ccgcccgaac actgggccgg gaacccatcg ctcaagcccc atttctacga
     2281 ccctcagcag gccaaatcgc tgctgcagca ggcgggggtg caactgccgc tgaagctggt
     2341 ctacaaaact tccaccgacg cccagcgcgt gcggctcgcc accatgatgc aggcacagat
     2401 gcgcgaggcc ggcatcgaag tggaaatccg cagcctcgat tggggcacgt tcttcgatga
     2461 cgtgaagcat ggtaaattcc agttgtacgg cttgacctgg gtcggcatca aaacgccgga
     2521 gatttaccgg ttggcctttc atagtacttc ggtgccacct gcgggggcca accgcgggcg
     2581 cttgcaggac gcactgctgg acgggctgat tgagaaacgg gattggccgg gagcgacgca
     2641 acgcatccat gaactgctgc cttacgttcc gctctggtac gaaggccagt ttgccgccat
     2701 gcgtagcagc gtgcaaggct atgcgcctgc gccggatggc aactgggacg cattggcaat
     2761 gattaccagg caacagacag gaaacgcaaa atgatcaaga tcaaccgtat gatgcacacc
     2821 ggcctgatta ttgccgattt gcagcgctca cgcgccttct acgaaggctt gctgggattt
     2881 acgcccaacc ccaaccgccc caaattcggc ttcgagggcg tgtggtacga catcggcgcc
     2941 aaccaggtgc acctgatggt ggtgcccaac ccctataccg gggtggttca cccggagcat
     3001 ggtgggcgcg acatccacat cgccttttcg gtggatgatg tggagcaggt gcgcgtcgag
     3061 ttggaaaagg caggcatccc atacagcatg agcaagtccg gccgcgccgc gctgttctgc
     3121 cgcgaccctg acggcaacgc gctggagttt tcggcggttt gatgcggatc gaggttttca
     3181 ttactcgcca gtccttgcgc ctgctggatg acgagaggct ggtgctggcg gattatcccg
     3241 tttctacggc cctcaacggt gccggcgagg ccaagggcag cggctgcacc ccgtgcgggc
     3301 ggcacgtggt gcgcgccaga atcggcgccg gccggccaga gggcaccgta ttcatcggac
     3361 gccgccccac cggcgaaatc tggtcgccgc aactggccgc gcagttcccc gagcgcgact
     3421 ggatcctgac gcgcatcctg tggctgtctg gctgcgagcc gggcatcaac cggctgggcg
     3481 acgtggatac catgcagcgt tacatttaca tccacggcac gccggacacg gagccgatgg
     3541 gcgtgccgcg ctcgcatggc tgcatccgca tgcgcaacgc cgatgtcatc gatctgttcg
     3601 acaaagtccc ggccggcacg ccggtgctga tttccgcttg aattccctgc tgaaaagact
     3661 ggtcggcgtg ctgccggtgg tgttcggcgt gctgctgctg acctttctgc tgattcatct
     3721 ggtacccggg gatccggttg aagtcatgct cggtgaatct gccgccgcgg ccgaccgcgc
     3781 ccaactgcgc gccgaccttg ggctggatcg tccgttgccg gtgcagtttg ccgattatct
     3841 ggtccggctt gcgcacggag atttcggccg ttccatccat agccgggatg aaatttcctc
     3901 acttctggca gagcgccttc cggccacggc gaagcttgca atatcggcgc tgctggttgc
     3961 actatgcatc ggcttgccgt tgggcatcgc tgcagcgctg aatcatcgcg gctggccgga
     4021 ccgcgtcgcg acgctgacca gtctgacgct ttccgccatg ccgcatttct ggctggggcc
     4081 gttgctgatg ctggtgtttg ccctctggct tggctggctg ccggtgagcg gcatggaggg
     4141 ctggacatcc atagtgcttc catcgttgac gctgggttca ggactggccg ctatcctgac
     4201 ccgcatgact cgtgcaagcc tgctggaggt gttgcacgag gattatgttc gtactgcccg
     4261 tgccaaggga ttgcctgaaa gaacggtgat tctgcgccac gccttgcgtg cggcgttgtt
     4321 gccggtggtg acggtggtcg ggctgcagct cggttttctg ctcaccggtg cggtgatcac
     4381 cgaaaccgtg ttcggctggg aaggcgtagg tcgcctgttg gtggagtcta tccagaagcg
     4441 cgattatccc gtttcgcaag cctgcgtgct ggtgattgcg ctgatctacg tggtggtgaa
     4501 tctgctgacc gacctggctt atgcgcggct ggacccaagg gtgagattca gggaatgaag
     4561 tcgctgccag ctgctgtttt ggttttctgg gttgctgttg ccctgatggc gccgctgctt
     4621 ggcatgcagc ccgacgccat tcatctggag cgcatccttt ctacgcctga tatgcacgcc
     4681 tggctgggga acgacgatct cggtcgcgat attctggcgc ggttgctggc tggcgcgcag
     4741 gtgtctctgg cggtggctgc catcgtgacc ggagtcacga tggtatttgg cacgacgctg
     4801 ggactggtgg caggttattt cggcggcggg gtggatcgcg ccctcatgca tgtcaccgat
     4861 gtgtttctcg cttttccagg cattctgctg gcgattgcgt tcgctgccgt gcttggaccc
     4921 ggtgtggaca atctggtact ggcactttgt ctcacttcct gggtcggcta tgcacgcttg
     4981 acgcgcgcac aggcgctggc gttgaaaggc cgccagcatg tgctggcggc gcggtcgttg
     5041 ggggcctcca cttctcgcat catgtttcgc cacatgttgc cgttgctggc tgcgcctttg
     5101 atcgtggagt cgacctacaa aattgccagc ctgatcattg ccgaagccag cctgtccttc
     5161 ctcgggctcg gcatccagcc gccacatgct tcgtgggggt ccatgttgcg cgacggggtg
     5221 cgctacctgc tggtggcgcc gcattacgtg ctggcggtag gggtgagcct gatgtcgctg
     5281 gtattggcgg ttaatctgct aggggataca ctgcgcgatt cctgggatgt gaaaaggtaa
     5341 atagggcgtg ctcacattaa tttcgcgtct gcgttgccaa gataacgcga tggatgctgt
     5401 tct
//
LOCUS       DAOMFA010000110         5253 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_2055_length_5253_cov_4.336860, whole genome shotgun sequence.
ACCESSION   DAOMFA010000110 DAOMFA010000000
VERSION     DAOMFA010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 5253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5253
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2055_length_5253_cov_4.336860"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1091
                     /locus_tag="PKW44_06385"
     CDS             <1..1091
                     /locus_tag="PKW44_06385"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HQR51247.1"
                     /translation="GLLKHPTFGWSHLDGGGPGKTTCGVELYFLGLLLAAPRQRIENV
                     RFKHTRLRVTQAVLRHAAETRKAWAEALAAVQTTGLLGQVNELAQVEADLAARQRKAG
                     NISERDLLLRQAFAAETRTAQLRAAETANAVRERLNRLMGLGNQHAAWTLPPRMPGLP
                     AEQPRFDDLENYGLRQRLDLQMVQKEAEAFSTALGLTRSTRFINVLDLGVETETNVGE
                     KRITGPTLRIELPIFDQGQARISKQEAQYRQSEMRLYDSVVVARSEIREAQQRLATAW
                     QTATEAREVLSPLRRRIVEQSALQYNGMLIGVYELLAAARDQVNAAQVQITAVRDYWT
                     ALADLQMAVGGKLPPESAAMAGQEGYKK"
     gene            1088..2356
                     /locus_tag="PKW44_06390"
     CDS             1088..2356
                     /locus_tag="PKW44_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper oxidase"
                     /protein_id="HQR51248.1"
                     /translation="MTTRRQFIAGGAALIGAAFAERAAAAPDIVSRSDPATESPLWLA
                     GGKAYQPVVTLNGWTLPPRLVGGVKEFHLVAEPVKREFAPGMTVNCWGYNGSTPGPTL
                     EAVEGDRVRIYVTNRLPEHTSIHWHGIRLPNGMDGVGGLTQPHIPPGKTFVYEFLLKR
                     SGTFMYHPHSDDMLQVAMGMMGLFIVHPKNPRERPVDRDFAFLLHAWDIDPGTYTPKS
                     ATMTEFNLWTFNSRVFPAIDPLVVRKGDRVRIRIGNLSMHDHPIHLHGYDVQVTGTDG
                     GWLPKSAQFPEITVNLPVGAMRAFEFVADEPGDWALHCHKLHHAMGPMGHAVPNMLGV
                     GQDGIAGKIGKLVDGYMAMGETGMAEMQEMQMPLPENTLPMMAGEGPFGPLEMGGMVT
                     LLMVREGLSHDDYRNPGWYKHPPGTVAWEV"
     gene            2396..4495
                     /locus_tag="PKW44_06395"
     CDS             2396..4495
                     /locus_tag="PKW44_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018506825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HQR51249.1"
                     /translation="MQTPLALNSYRPYWAKRFGTAPFLPMSRAEMEALGWDSCDVILV
                     TGDAYIDHPSFGMALVGRLLEAQGFRVGIIAQPDWQSAEPFKALGKPNLYFGVTAGNM
                     DSLVNRYTADRKIRSDDAYTPEGVAGKRPDRAVLVYSQRCREAYPDATLVIGSIEASL
                     RRIAHFDYWSDKVRRSVLVDSKADILLYGNAERALVDLTHRLAKGEPVANITDLRGTA
                     FLRKSIPEGWVEMDSSDMDTPGAVHPHPDPYAMEAAGAAPRRDSGCAIVPGAALPLTF
                     QRKPKLDRAHTVVRLPSYELVSRDPVMYAHASRVLHLESNPGNARALVQRHGDRDVWL
                     NPPPIPLTTKEMDYVYDLPYARGPHPGYKGAKIPAWEMIRFSVNIMRGCFGGCTFCSI
                     TEHEGRIIQSRSEESILHEIEEIRDKVDGFTGIISDLGGPTANMYRLACKDPRIESAC
                     RKLSCVYPDICDNLNTDHSALVQLYRKARQIKGVKKILIGCGLRYELAVKSPEYVREL
                     VQHHVGGYLKIAPEHSEENVLSMMMKPGMGAYDKFKAMFEKFSKEAGKEQYLIPYFIA
                     AHPGTTDEDMLNLALWLKRNNFRLDQVQTFTPTPMAMATAMYHSGKNPLRKVTKTSED
                     VPIPKAGGKRRLHKAFLRYHDPVNWPLLREALKAMGRADLIGSGKKHLVPAWQPLAVA
                     KGTERRRPMTGRPGKPR"
     gene            4566..4641
                     /locus_tag="PKW44_06400"
     tRNA            4566..4641
                     /locus_tag="PKW44_06400"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4599..4601,aa:Arg,seq:cct)
     gene            complement(4788..>5253)
                     /locus_tag="PKW44_06405"
     CDS             complement(4788..>5253)
                     /locus_tag="PKW44_06405"
                     /inference="COORDINATES: protein motif:HMM:NF013555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HQR51250.1"
                     /translation="TVADFLLSMHRPLSRADSAVPESLPTLEITPAEMPGKGFVPKVG
                     AVCPQCEKHKLTRKSVARSNGTETDFLACEGYPSACKAIFALVAIARPPKKQAAESSI
                     TDSPAIIEPKYRENDPCPRCKKGTLVSRNGKTKFLGCSSYPKCKFTDYRDQG"
BASE COUNT         1056 a         1616 c         1638 g          943 t
ORIGIN      
        1 ctggcctgct gaagcacccg accttcggct ggagtcatct tgacggcggc gggccgggca
       61 agaccacttg cggcgtggag ctgtattttc ttggcctgct gctggccgcg ccccggcagc
      121 gcatcgagaa tgtgcgcttc aagcacacca ggctgcgcgt gacgcaagcg gtgctgcgcc
      181 acgcagcgga aacgcgcaag gcctgggccg aggcgctggc ggccgtacaa accaccggcc
      241 tgctgggtca ggtaaacgag ctggcgcaag tggaagccga cctggctgcg cgccagcgca
      301 aggccggcaa catcagcgag cgcgacctgc tgctgcggca ggcgttcgcc gccgaaacgc
      361 gcacggcgca actccgcgcc gccgaaactg ccaatgccgt gcgcgaacgc ctcaaccgcc
      421 tgatggggct gggcaaccag catgccgcct ggacactccc gccacgcatg ccgggactgc
      481 ccgccgaaca gccaaggttt gacgatctgg aaaactacgg gctgcggcag aggctggacc
      541 tgcagatggt gcagaaagag gcggaggcat tttccaccgc gctcgggctg acgcgcagca
      601 cgcgcttcat caacgtgctc gacctcggcg tggaaaccga aaccaacgtc ggtgagaagc
      661 gcatcaccgg cccgaccttg agaatcgaat tgccgatttt cgaccagggg caggcgcgga
      721 tttcgaaaca ggaagcgcag taccggcaga gcgagatgcg gttgtacgac agcgtggtgg
      781 tcgcgcgttc ggagattcgc gaggcgcagc agcgcctggc cacggcctgg cagacggcga
      841 ccgaagcgcg cgaggtactc tcgccgctgc gccgccgtat cgtggagcaa agcgctctgc
      901 agtacaacgg catgctgatc ggcgtctacg agctgctggc tgccgcccga gatcaggtga
      961 acgcagccca agtccagatc actgccgtgc gcgattactg gacggcattg gcggatttgc
     1021 agatggcggt gggcggcaag ttgccgccgg aatccgctgc aatggccggg caggaaggat
     1081 ataaaaaatg acgacacgca gacaatttat cgccggggga gccgccttga tcggcgccgc
     1141 ttttgccgag cgcgcggccg ccgcgccgga tatcgtcagc cgcagcgacc ccgcaaccga
     1201 gtcgccgttg tggctggccg gcggcaaggc ttatcaaccc gtcgttacgc ttaacggctg
     1261 gacactgccg ccgcgcctgg ttggcggtgt caaggagttc catctggtgg cagagccggt
     1321 caagcgcgag ttcgcgcccg gcatgacggt gaactgctgg ggctataacg gcagcacgcc
     1381 gggcccgacg ctcgaagcgg tggagggcga ccgcgtgcgc atctacgtca ccaacaggct
     1441 gccggagcat acctccatcc actggcacgg catccgcctg cctaacggca tggacggtgt
     1501 gggtgggctg acgcagccgc acatcccgcc gggcaagacc ttcgtctacg agtttctgct
     1561 caaacgcagc ggcaccttca tgtaccaccc gcattcggac gacatgctgc aagtggcgat
     1621 gggcatgatg ggtctgttca tcgtgcaccc gaagaacccc agggaacggc cggtggaccg
     1681 cgattttgcc ttcctgctgc acgcctggga catcgacccc ggcacctaca cgccgaaatc
     1741 tgccaccatg accgagttca atctgtggac gttcaacagc cgcgtgttcc cggccatcga
     1801 tccattagtg gtgcgcaaag gcgaccgcgt gcgcatccgc atcggtaatc tcagcatgca
     1861 cgaccatccc atccacctgc acggctacga cgtgcaggtg accggtaccg acggcggctg
     1921 gctgccgaag agtgcgcagt tccccgagat cacggtcaac ctgccggtgg gcgcgatgcg
     1981 ggcgtttgaa ttcgtggcgg acgagccggg cgactgggcg ctgcactgcc acaagctgca
     2041 ccacgccatg ggcccgatgg ggcatgccgt gcccaacatg ctgggggtgg gccaggacgg
     2101 catcgccggc aagatcggca aactggtgga tggctacatg gcgatgggcg agaccggcat
     2161 ggcagagatg caggaaatgc agatgccgct gccggagaac actctgccga tgatggcggg
     2221 cgaggggccg ttcggcccgc tggaaatggg cggcatggtc acgctgttga tggtgcgtga
     2281 agggttgagc cacgacgact accgcaatcc cggttggtac aagcatccgc caggcacggt
     2341 ggcgtgggaa gtttagcagg cggtcagggg atatttcctg taaaatcacg cacctatgca
     2401 aactccgctg gcactcaact cctaccgtcc ctactgggcc aagcgcttcg gcacggctcc
     2461 attcctgccg atgtcgcgcg ccgagatgga ggcgctcggc tgggattcgt gcgatgtgat
     2521 cctggttacc ggcgacgcct acatcgatca tcccagcttc ggcatggcgc tggtggggcg
     2581 gctgctggag gcgcagggtt tccgcgtcgg catcattgcc cagccggact ggcagtcggc
     2641 agagccgttc aaggcgctgg gcaagcccaa cctgtatttc ggcgtgacgg cgggcaacat
     2701 ggattcgctg gtcaaccgct acaccgccga ccgcaagata cgctccgatg acgcctacac
     2761 gccggaaggg gtcgcgggca agcggcctga ccgtgccgtg ctggtgtact cgcagcgctg
     2821 ccgcgaagcc tatcccgatg ccacgctggt gatcggcagc atcgaagcca gcctgcgccg
     2881 cattgcccac ttcgactact ggtcggacaa ggtgcggcgc tcggtgctgg tggactccaa
     2941 ggccgacatc ctgctctacg gcaacgccga gcgtgccctg gtggatttga cccaccggct
     3001 ggccaagggt gagccggtcg ccaacattac cgatctgcgc ggtacggctt tcctgcgtaa
     3061 aagcatcccc gaaggctggg tggaaatgga ttcctcggac atggatacgc cgggagcggt
     3121 gcatccgcat cccgatcctt atgcgatgga ggctgcggga gcggcgcctc gccgcgattc
     3181 aggttgtgca attgtgccgg gggcggcgct ccccctgaca tttcagcgca agcccaagct
     3241 ggatcgtgcc cataccgttg tgcgcctgcc atcctatgag ctggtgagcc gcgatccagt
     3301 catgtacgcc cacgcctcgc gcgtgctgca cctggagtcc aatcccggca acgccagggc
     3361 gcttgtccaa aggcacggcg accgcgacgt gtggctcaac ccgccgccga tcccgctgac
     3421 gacgaaggag atggattacg tctacgacct gccctacgcg cgcggcccgc atccaggcta
     3481 caagggagcg aagattccgg catgggagat gatccgcttc tcggtcaata tcatgcgcgg
     3541 ctgcttcggt ggctgcacct tttgctccat caccgagcac gaaggccgca tcatccagag
     3601 ccgctcggaa gaatccatcc tgcacgagat cgaggaaatc cgtgacaagg tcgatggctt
     3661 caccggcatc atctccgatc tgggtgggcc gactgccaac atgtatcgcc tggcgtgcaa
     3721 ggaccccagg atcgaatccg cctgccgcaa gctgtcctgc gtctacccgg acatctgcga
     3781 caacctcaac accgaccatt cggcgctggt gcagttgtac cgtaaagccc gccagatcaa
     3841 gggtgtgaag aaaatcctga tcggctgcgg gctgcgctac gagctggcgg ttaaatcgcc
     3901 ggaatacgtc agggagctgg tgcagcacca tgtcggcggc tacctcaaga tcgcccccga
     3961 acacagcgag gaaaacgtgc tgtcgatgat gatgaagccg ggtatgggcg cctacgacaa
     4021 gttcaaggcc atgttcgaga agttcagcaa ggaggccggc aaagaacagt acttgattcc
     4081 gtacttcatc gccgcccatc ccggtaccac ggacgaagac atgctcaatc tcgccttgtg
     4141 gctcaagcgc aacaacttcc gcctcgacca ggtacagacc ttcaccccga cgccgatggc
     4201 gatggccacc gccatgtacc attccggcaa gaacccgctg cgcaaggtga ccaagaccag
     4261 cgaggacgtg cccatcccca aggccggcgg caagcgccgg ctgcacaagg ccttcctgcg
     4321 ttaccacgac cccgtcaact ggccgctgct gcgagaggcg ctgaaagcga tgggccgggc
     4381 ggacctgatc ggtagcggca agaagcatct ggtgccggcc tggcagcctc tggctgtcgc
     4441 caagggcacg gaacgcaggc gcccgatgac aggccgcccc ggcaagccca gataacttaa
     4501 gccggcaggc aaaaagcgct cgccaaatag atttttaggt gtgcccgtga gaaaatgccg
     4561 gcctcgcccc cgtagctcag tggatagagc atccccctcc taaggggagg gtcacacgtt
     4621 cgattcgtgt cgggggcgcc aaataaaaca atggcttgca gattaacagc cgcaagccat
     4681 tttgcttatt tatttactct tcaagggtaa cccagataag cgcttggggg attaattcat
     4741 tctcccatcg cagtcaattt tcttcgatga aaatcagtgc ctcaaaatca gccttgatca
     4801 cgatagtcag taaatttaca tttcggatag gacgagcagc caagaaactt ggtttttcca
     4861 tttcgactga ctagagttcc ctttttgcac cttgggcaag ggtcgttttc cctgtattta
     4921 ggctcaatga tggcaggtga atcagtaatg ctagattctg ccgcctgttt ttttgggggc
     4981 cttgcaatag caaccaaagc gaaaatcgct ttgcatgctg atggatagcc ctcacaggcc
     5041 agaaagtcag tttcggtgcc attacttcgt gcaacacttt ttcttgtcag cttgtgcttt
     5101 tcacattgcg ggcagacggc tcctaccttt gggacaaagc cttttcctgg catttctgcg
     5161 ggcgtgattt caagagttgg caggctttcc ggcactgcac tgtctgcacg agataggggg
     5221 cgatgcattg aaagaagaaa atcagcaaca gtt
//
LOCUS       DAOMFA010000111         5202 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_2089_length_5202_cov_4.577035, whole genome shotgun sequence.
ACCESSION   DAOMFA010000111 DAOMFA010000000
VERSION     DAOMFA010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 5202)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5202)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5202
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2089_length_5202_cov_4.577035"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            63..1067
                     /gene="pbpG"
                     /locus_tag="PKW44_06410"
     CDS             63..1067
                     /gene="pbpG"
                     /locus_tag="PKW44_06410"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013146984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HQR51251.1"
                     /translation="MRKFLSLCCAVALAFPVYAAEAATQKPAKHKTVVKSKDKAGKHS
                     VSSRRIIHTIRSDSKYRVRTVNYQPRHNVFVEEGDGSELRLASSKALIINQDTGEVLY
                     AKSTDVPTPIASVTKLMTAMVVLDAHQPLTEPITISEAEVDTLKGTGSRLRVGTTLTR
                     GEMLQLALMASENRAAASLGNAYPGGRQAFVAAMNAKAVALGMTRSRFVDGTGLNSEN
                     VSTAEDLAKMVKAAYQYPEIRQVTTTSEYEVPVHGLSRPVEFRNTNILVRNSDWQIGL
                     SKTGFINEAGRCLVMQAQIADQPLIIVLLDSWGKFTRIGDAQRIRKWIESSNGLRKLG
                     "
     gene            complement(1129..3657)
                     /gene="topA"
                     /locus_tag="PKW44_06415"
     CDS             complement(1129..3657)
                     /gene="topA"
                     /locus_tag="PKW44_06415"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020183595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HQR51252.1"
                     /translation="MSKLLIVESPSKAKTLKKYLGKDFEVLASYGHVRDLIPKTGAVD
                     TEHDFAMKYEIIDRNARHVDAIAKAVKAADAIYLATDPDREGEAISWHIAEILKGKKL
                     LKDKLLKRVEFHEITKSAVEHAIAAPRDISMPLVNAQQARRALDYLVGFNLSPLLWKK
                     IRRGLSAGRVQSPALRLIVERELEIEAFKRQEYWTIHLQSRKKQQAFDAKLVQLAGSK
                     VEQFTVTSQDQQAEIVGKLLAASAGKTTVSRVEKKQKNRSPAAPFTTSTLQQEAVRKL
                     GFTTSRAMRIAQQLYEGVSIGAEGTVGLITYMRTDSFSMAAEAVMQIRDYIKGHFEAD
                     YLPKSPIMYKTKAKNAQEAHEAIRPTDIRRTPDSLRNHLTPEQFRLYDMIWKRALACQ
                     MAPARFDAVSVDLTVGSEANLFRATGQTMIFPGFIAVYMEGLDDTEEESESKLPVLEV
                     GEMLDVTKIWGDQHFTEPPPRYSEASLVKALEEYGIGRPSTYASIISTLQDREYVLLD
                     NKRFTPTDVGRIVNKFLTEHFTKYVDYDFTANLENELDEIAEGGREWIPVLDEFWQGF
                     NRQIADKAEVERPGTEVLDEACPKCGKPLSKRLGKRGSFIGCTGYPECDYTRNLDGDA
                     PAGNEPVTVGADPASGKDILLLNGPYGPYLQLGQTADDPKKKPKRVSIPKEIPLASVS
                     LDVALKLIALPREIGLHPETGKKITAGIGRFGPYLNHNGTFKSIPRSDSIFDVDLPRA
                     VELLAQAKAKAAPLRELGEHPTEGGNIAIYAGRYGPYVQHGKVNATLPKDEAVETVTL
                     EQALQLLAAKTAKGAPAKKTSSARKAPAKKPALKKPAAARKKAG"
     gene            3921..4049
                     /locus_tag="PKW44_06420"
     CDS             3921..4049
                     /locus_tag="PKW44_06420"
                     /inference="COORDINATES: protein motif:HMM:NF022790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3149 domain-containing protein"
                     /protein_id="HQR51253.1"
                     /translation="MLKVLFGSWVGIASLTAVVLTIVAVSAVLGFCIWNEEKTRNS"
     gene            complement(4046..>5202)
                     /locus_tag="PKW44_06425"
     CDS             complement(4046..>5202)
                     /locus_tag="PKW44_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HQR51254.1"
                     /translation="YAEKYKEILARQGITLELQTSTGSPQNLERLADEDNPVTIAFVQ
                     GGIDTPVGGEYNMTLGSMYYEPLWVFYRGDETYEQLTQLRGKRLAIGTVGSGNHKLVM
                     QILTANNMQGDSGNHLALGGDDAAKALLQGEVDAALIIAGADSAVVRKLLNTDHVRLM
                     SFAQADAYVTMFPFLYKVTLPRGGASLPHNLPGRDITLVATPANLIARHSTHPALVSL
                     IAGAAQEVHGKSGMFQRAGEFPAMKDKTFQVNTDAERYYKSGPPFLERYLPFWIAVLV
                     DRIWVMIIPLLALIPILRLLPGVYRWRVTSRINRWYGVLSALELEIRHQYDPSRQAEY
                     MAKIDVIEERANSYPIPVAYAHLGYTLREHINLVRAALERKSGKQNLPKG"
BASE COUNT          937 a         1436 c         1607 g         1222 t
ORIGIN      
        1 taaatgattt tgttatgatg gctcacgatt attggcttta acaatcggaa gggggttcaa
       61 ttctgcggaa atttctatcg ctttgctgcg cagtagcgct ggcattccct gtctacgcgg
      121 cagaagctgc cacgcaaaag ccggccaagc ataagactgt tgtaaagagc aaggacaagg
      181 ccggaaaaca ttcggtttcc agccgcagga tcatccacac catccgttcg gacagcaaat
      241 atcgggtacg tactgttaat tatcagccgc gacacaatgt ttttgtcgag gagggcgatg
      301 gtagcgagtt gcgccttgct tcctccaagg cgctgatcat caaccaggat acgggtgagg
      361 tgctctacgc caagagcacc gacgtcccta ccccgattgc ttcggttacc aaactgatga
      421 cggcgatggt ggtgctggat gcacatcagc ctttgactga accgattacc atctccgagg
      481 cggaggtgga tacgctgaag ggcaccggtt cgcgtttgcg tgtcggaacc acgctgacgc
      541 gcggcgaaat gttgcaactg gcattgatgg cttcagagaa tcgcgccgcg gcgtcgctag
      601 gtaacgctta tcccggcggg cgccaggctt tcgtcgctgc catgaatgcc aaggctgtgg
      661 cactcggcat gacgcgctcg cgttttgtag atggcaccgg ccttaacagc gagaacgttt
      721 cgactgcgga agacttggcc aaaatggtca aggctgccta ccagtacccg gaaatccgcc
      781 aggtcacgac aacctcggag tacgaagtac cagtacatgg cctgagccgg ccggtcgaat
      841 ttcgcaacac caatatcctg gtgcgcaaca gcgattggca gatcggcctg tccaaaaccg
      901 gcttcatcaa cgaagccggt cgttgtctgg tgatgcaggc gcagattgcc gaccagccgc
      961 tgatcatcgt cctccttgat tcctggggaa aatttacccg catcggcgat gcgcagcgca
     1021 ttcgtaaatg gatcgaaagc agtaacggct tacgtaagtt gggttgacgc catttctgag
     1081 ggggcaatag aaaagccggg actccccggc ttttttattg ccctgacact aaccagcctt
     1141 ttttcgagcc gcagctggtt tttttagtgc cggttttttg gctggagctt ttctggcgga
     1201 ggatgtcttt ttcgcgggcg cacctttggc ggtcttggca gcgagaagtt gcaatgcttg
     1261 ctcgagcgtc actgtctcga ctgcctcgtc tttgggcagc gtggcgttga ccttgccgtg
     1321 ctgcacgtag gggccgtaac ggccggcata aatggcgatg ttgccgcctt ctgtcggatg
     1381 ctcacccagt tcgcgcagcg gcgcggcctt ggctttggcc tgtgccagca gctctaccgc
     1441 gcgcggcagg tcgacgtcga aaatgctgtc cgacctggga attgatttga acgtgccgtt
     1501 gtggttcagg tatgggccga agcggccgat gccggcggtg atcttcttgc cggtctccgg
     1561 gtgcagaccg atttcgcggg gtagtgcgat cagtttcagc gcaacgtcca gagagacgct
     1621 tgccagcggg atttccttgg gaatgctgac gcgttttggt tttttcttgg ggtcgtccgc
     1681 agtctggcct aattgcaagt atggcccata cgggccgttg agcaacagga tgtccttgcc
     1741 gctggccggg tctgcaccaa cggtcaccgg ctcgttgcca gctggtgcat cgccgtccag
     1801 gttgcgtgtg tagtcgcatt ccgggtagcc ggtgcagccg atgaagctgc cgcgcttgcc
     1861 caggcgcttg gacagcggct tgccgcactt ggggcaggct tcgtccagca cttcggtgcc
     1921 ggggcgctcc acttcggcct tgtccgcgat ctggcggttg aagccctgcc agaattcgtc
     1981 gagcaccgga atccactctc gtccaccctc ggcgatttca tccagctcgt tttccaggtt
     2041 ggcggtaaag tcgtaatcga catacttggt gaaatgttct gtcaggaact tgttgacgat
     2101 gcggccgacg tcggtaggcg tgaatcgctt gttgtccagc agcacatact cgcgatcctg
     2161 cagcgtcgag atgatgctgg cgtaggtgga agggcggccg ataccgtatt cttccagtgc
     2221 cttgaccagg ctggcttcgg agtagcgtgg cggcggctcg gtaaagtgct ggtcgcccca
     2281 gattttcgtc acgtccagca tctcgcctac ctccagcacc ggcagtttgc tctcgctctc
     2341 ctcctcggtg tcgtccaggc cttccatgta gacggcgata aagccgggga aaatcatggt
     2401 ctggccggtg gcgcggaaca ggttggcttc actgcccacg gtgagatcga cgcttacggc
     2461 gtcaaatctg gcgggcgcca tctggcaggc cagcgcgcgc ttccagatca tgtcgtagag
     2521 gcggaactgc tccggcgtca ggtggttgcg caggctgtca ggcgtacgac ggatgtcggt
     2581 ggggcggatt gcctcgtgtg cttcctgcgc gtttttggcc ttggtcttgt acatgatcgg
     2641 cgatttcggc aggtaatccg cctcgaaatg gcccttgata tagtcgcgga tctgcatgac
     2701 cgcctccgcc gccatgctga aggagtcggt gcgcatatag gtgatgagcc ccacggtgcc
     2761 ctcggcgccg atgctgacgc cttcgtacaa ctgttgggca atgcgcatgg cgcggctggt
     2821 ggtgaagccc agcttgcgca ccgcctcctg ttgcagcgtg gaggtggtga atggtgccgc
     2881 ggggctgcgg ttcttctgct tcttctcgac gcgcgaaacc gtggtcttgc ctgcgctcgc
     2941 cgccagcagc ttgccgacga tctcggcctg ctggtcctgg ctggtgacgg tgaactgctc
     3001 caccttgctg ccggccagct gcaccagctt ggcgtcgaac gcctgctgct tcttgcggct
     3061 ttgcaggtga atcgtccagt attcctggcg tttgaatgcc tcgatttcca actcgcgctc
     3121 gacgatcagc cttagcgcag ggctttgcac gcgcccggca gaaaggccgc ggcggatctt
     3181 cttccatagc agcggcgaca ggttgaagcc caccaggtaa tccagcgccc ggcgtgcctg
     3241 ttgcgcgttg accagcggca tggaaatatc gcgcggcgcc gcgatggcat gctccaccgc
     3301 actcttggtg atctcgtgga actccaccct cttgagcagc ttgtccttga gcaacttctt
     3361 gcccttgaga atctcggcga tatgccagga gatggcctcc ccttcgcggt ccgggtcggt
     3421 cgccaggtag atggcgtcag ccgccttgac cgccttggcg atagcatcga catgccgcgc
     3481 gttgcggtcg atgatttcgt acttcatggc gaaatcgtgc tcggtatcca ccgcgccggt
     3541 cttgggaatc aagtcgcgca cgtggccgta ggaggccagc acctcgaaat ccttgcccaa
     3601 gtacttcttc agtgttttgg ccttggaagg cgattcgacg atcagcagtt tcgacattta
     3661 ttcagcgcga gtggggagca agagcggcag ataataagag caaacgccag caaagggcaa
     3721 gaaagtgttt tttgtgtcag gcggttcagc gcgtgaattc aaccaacgtt gtcccggttt
     3781 ctgggaagta ttgttggata taccggcttt tggacgtcca taatcgacga taaacgatca
     3841 tccagagtta gacctcttgc attaaagact aataagatta gaatatccta atattattat
     3901 tttactgaag gggcttgatc atgttgaaag tgctttttgg ttcttgggtt gggattgctt
     3961 ctctcacggc ggttgtgctc actattgtgg cagtcagcgc cgtgcttgga ttctgcattt
     4021 ggaatgaaga gaagacacgt aatagctaac ccttaggcag attttgcttg cccgacttcc
     4081 gctccagggc ggccctgacc agattgatgt gctcacgtag cgtgtagccg agatgggcat
     4141 aggccaccgg gatcgggtag ctgttggcgc gttcctcgat gacgtcgatt tttgccatgt
     4201 attcggcttg gcgcgatgga tcgtactggt gcctgatctc aagctcgagc gcggaaagca
     4261 ccccatacca gcggttgatg cgtgaagtga cgcgccatcg gtagacgccg ggtagcagcc
     4321 gcaggatggg gataagtgcc agcaggggga tgatcatgac ccagatccgg tccaccagta
     4381 ccgcgatcca gaacggcagg taacgttcca ggaagggtgg ccccgatttg taatagcgtt
     4441 cggcatcggt gttgacctgg aacgtcttgt ctttcatcgc ggggaattcg ccggcccgct
     4501 ggaacatgcc gctcttgccg tgcacttcct gcgcggcgcc tgcaattagg ctgaccagcg
     4561 ccggatgggt gctgtggcgt gctatcaggt tggcgggggt ggcgacaagg gtaatatccc
     4621 ggccaggcag gttatgtggc aggctcgctc cgccgcgtgg cagcgtgacc ttgtaaagga
     4681 aagggaacat ggtgacgtag gcgtcggcct gggcgaagct catcaggcgc acgtggtcgg
     4741 tattcagcag cttgcgtacc acggccgaat ccgcgccggc aatgatgagt gccgcgtcca
     4801 cttcgccttg cagcaaggct ttggcggcat cgtcgccacc caatgccaaa tggtttccgc
     4861 tgtcgccttg catgttgttg gcggtcagga tctgcatgac cagcttgtgg tttccgctgc
     4921 cgacggtgcc gatggccagg cgcttgcctc gcagctgtgt cagctgttca taggtttcgt
     4981 cgccccggta gaaaacccag agcggctcat agtacatgct gcccagcgtc atgttgtatt
     5041 cgccgcccac cggggtgtcg atcccgccct gtacgaaggc gatggtgact gggttgtcct
     5101 cgtccgccag ccgttccagg ttctgcgggg agccggtgga ggtttgcagt tccagggtga
     5161 tgccctggcg tgccaggatt tccttgtatt tttcggcata gg
//
LOCUS       DAOMFA010000112         5163 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_2104_length_5163_cov_4.595341, whole genome shotgun sequence.
ACCESSION   DAOMFA010000112 DAOMFA010000000
VERSION     DAOMFA010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 5163)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5163)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5163
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2104_length_5163_cov_4.595341"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            51..1811
                     /locus_tag="PKW44_06430"
     CDS             51..1811
                     /locus_tag="PKW44_06430"
                     /inference="COORDINATES: protein motif:HMM:NF023295.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3387 domain-containing protein"
                     /protein_id="HQR51255.1"
                     /translation="MNEDLYRLIEYDELDSDQEAKLEKVLGRQYHLITRDDRLETVAQ
                     DIVRHFLGRGFVGKAMVVSIDKATALKVHDKVKKYWVAETSRVQKELGELAYQPGSGM
                     TPEQARRDVRRAELKQRLSVLTSTDMALIVSPGQNEIQQMQKLGLDIEPHRRRMNESQ
                     PGLDEKFKDTDDPLRLVFVCAMWLTGFDAPSCSTVYLDKPMRNHTLMQTIARANRVFP
                     GKHSGVIVDYANVFASLEKALAIYGAGKDGKSPVKDKQQLVGELRKAVADATAFCAAH
                     KVMLAGIEALAAGSLERLQHIEDAMNALISPDPLRRDFFAHERLVSTLYQAVKPDPSA
                     LEFASPVACLTTLAEAIRARLNPNPPDISQVMGQIVGLLDESITGHEIREQGPPALDL
                     SRINFEALAQRFKQSKHKNTDLEVLKAAIRAQLEKMIQFNRTRADFAEKFEALIESYN
                     AGSRSIEELFDELVKLSNSLNDEQERHVRENMSEEELVIFDILTRPAPELSAEERAEV
                     KKAARELLVRLKALLVLNWRQKSTARSQIKLAIEDTLDTDLPRAYTPELYNQKCSAVF
                     EHVYESYQGDGRSVYDAVSA"
     gene            1753..3549
                     /locus_tag="PKW44_06435"
     CDS             1753..3549
                     /locus_tag="PKW44_06435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR51256.1"
                     /translation="MSTKAIRVTGGVFTMPYPRSDLLRALLATLACLAIAFPAFAVSR
                     IDIHVGGISYSGAEVENVAAKLDMDGGWQGKATLKQSDLAQLAKQQSLPVAVSKGNAQ
                     GQVSFSGVAGNLLQFRLEAALRDIAFSDAEGRRAGEALDGRISLAAQRSGERWHWQGL
                     LDWTAGELYWQPLYFANGGHVLNADGWLSADTLAVEQGELSLAEIGRVAFNGRLSLAD
                     KTLASLQLSANDLHAGNAYTLLLRPFLQKGTFGDLAMSGQVDFQADISNQRVKNFEVS
                     LRDLDVEDSRRRFALYKLNAHIPWGYDDKTRAWVRYDGGQLLGLRLGAARHQVTLERY
                     SLTAPHLDLPLLDGVLVMNDVAAALVNDRWHWRVRADMQPVSMAELSQALGWPRMEGK
                     VAMHIPMMYYTGGQLSADGDLRFEVFDGTVAVKGLAMQEPLGLAPRLSADLEMRNLDL
                     GLLTSTFSFGAIQGKLDGDVKGLALSSWQPVKFDASFRSSPGDYPKKISQRAVENISA
                     LGGAGAAVAIQRSFLRFFKEFNYASIGLSCKLDNGVCAMDGIAPAQSGYVIVKGSGIP
                     AITVLGYNRSVNWGELLERVQRITHGNKPVIK"
     gene            3562..3738
                     /locus_tag="PKW44_06440"
     CDS             3562..3738
                     /locus_tag="PKW44_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR51257.1"
                     /translation="MKNWVWLLPATLILPACVTINIYFPAAAAEKAADKIIDEVWQIK
                     PGEQQPAEKPAETK"
     gene            3763..4164
                     /locus_tag="PKW44_06445"
     CDS             3763..4164
                     /locus_tag="PKW44_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HQR51258.1"
                     /translation="MTVFVLLLALAAPLAMAAANLEINTPAITSLTASMQARYNQLSP
                     YYASGAIGLTRDGLVAVHDANTVPLKDRQVVNALVAAENNDRNALYKEIAIANGHPEW
                     EAEIRSTFAQRWVQKAQGGWWYQGAGGWAKK"
     gene            4161..4934
                     /locus_tag="PKW44_06450"
     CDS             4161..4934
                     /locus_tag="PKW44_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HQR51259.1"
                     /translation="MTPTLVFDIETIPDTDGLKKLLDLPAETPAADVASIAFHQRHQA
                     GQSEFLPLHVQRVAAISCALREGDNFRVWSLGGPEDGEGALIQRFFDGIDKYTPTLVS
                     WNGSGFDLPVLHYRGMIHNVVAPRYWDMGEDDREFKWNNYISRYHMRHTDLMDLLALY
                     QGRGNAPLDQLAQLCGFPGKLGMDGSKVWGAWQAGEIEAIRNYCETDVVNTWLVYLRF
                     QQMRGALSPAAYQREITLVRETLQDTPGQHWQEFLSNWA"
     gene            4985..>5163
                     /locus_tag="PKW44_06455"
     CDS             4985..>5163
                     /locus_tag="PKW44_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HQR51260.1"
                     /translation="MSFNPPTIVESLDQEGRGVTHVDGKAIFIEGALPGEKVTYSSYR
                     KKASYEFAHLESLLKP"
BASE COUNT         1044 a         1558 c         1644 g          917 t
ORIGIN      
        1 cgctgttcta cgagaaacgc cccccgtaat tgcagctcag caacccggac ttgaacgaag
       61 acctctaccg cctgatcgag tatgacgaac tagactcgga tcaagaggcc aagctggaga
      121 aggtgctggg gcggcaatac cacctcatca cccgcgacga ccgtctggaa acggttgcgc
      181 aggacatcgt gcggcatttc cttggtcgtg gcttcgtcgg caaggcgatg gtggtgtcca
      241 tcgacaaggc taccgcgctc aaggtgcacg acaaggtcaa aaaatactgg gtagcggaaa
      301 cgtcccgggt gcagaaagaa ttgggcgaac tggcctacca gcccggcagc gggatgacac
      361 ccgagcaggc gcgacgcgat gtgcgaaggg ccgagctgaa acagcgactg tcggtactga
      421 ccagcaccga catggcgctc atcgtttcac cggggcagaa cgaaatccag cagatgcaga
      481 agctgggact ggacatcgaa ccgcaccgca ggcgcatgaa cgagtcccag ccggggctgg
      541 acgagaaatt caaggacacc gatgaccctt tgcgcctggt gttcgtgtgc gccatgtggc
      601 tgaccggctt cgacgcgccg agctgctcga cggtgtatct cgacaagccg atgcgtaacc
      661 acacgctgat gcagaccatc gctcgcgcta accgcgtctt ccccggcaag cacagcggcg
      721 tgatcgtcga ttacgccaac gtcttcgcgt cgctggaaaa ggcgctggcg atctacggcg
      781 ccggcaagga cggcaagagc ccggtcaagg acaagcagca actggtcggg gaactacgca
      841 aggcggtggc ggatgccacg gctttctgcg ccgcacacaa ggtgatgctg gccgggatcg
      901 aggcgcttgc cgctggcagc ctggaacgtt tgcagcatat cgaggacgcc atgaacgcgc
      961 tgatctcccc cgatccgctg cgccgtgact tcttcgccca cgagcgactg gtgagcacgc
     1021 tctatcaggc tgtcaaacca gatccctcgg cgctggaatt tgccagcccc gtcgcctgcc
     1081 tgaccacact ggccgaggcc atccgcgcca ggctgaaccc caacccgccg gacatttccc
     1141 aggtgatggg tcagatcgtc ggcttgctcg acgaatccat caccggccac gaaatccgcg
     1201 agcaggggcc gccggcgctt gacctctcca ggatcaactt cgaggcgctg gcgcagcgct
     1261 tcaagcaatc gaagcacaag aacaccgacc tggaagtgct caaggcggcg atccgtgccc
     1321 aactggaaaa gatgatccag ttcaaccgca cccgcgccga cttcgccgag aagttcgagg
     1381 cgctgatcga gagctacaac gccggcagcc gcagtatcga ggagctgttc gatgaactgg
     1441 tgaagctctc caacagcctg aacgacgagc aggagcggca tgtgcgcgag aatatgagcg
     1501 aggaggaact ggtcatcttc gacatcctca cccgcccggc gccggagttg tcggcagagg
     1561 aacgcgccga ggtgaaaaag gctgcccgcg aactgctggt aaggttgaag gcactgctgg
     1621 tgttgaactg gcggcagaaa tccaccgctc gctcgcagat caagctcgcc atcgaagata
     1681 ccctcgacac cgacctgccg cgcgcctata ccccggagct ctacaaccag aagtgctcgg
     1741 cggtgttcga gcatgtctac gaaagctatc agggtgacgg gcggagtgtt tacgatgccg
     1801 tatccgcgta gtgatctgct ccgggcattg ctcgcaacac tggcttgttt ggcgatagct
     1861 tttccggcct ttgccgtcag ccgcatcgac atccacgtcg gtggaatctc ttactccggt
     1921 gccgaggtcg agaatgtcgc cgccaagctc gatatggacg ggggctggca gggcaaggcc
     1981 acgctcaagc agagcgatct ggcacaactg gcaaagcagc agtctctgcc ggtggcggtg
     2041 agcaagggca atgcgcaagg gcaggtcagc ttttccggcg tggccgggaa tctgctgcag
     2101 ttcaggctgg aggcggcgct gcgagacatt gccttcagtg acgcggaagg ccggcgtgcc
     2161 ggggaagcac tggacgggcg tatcagtctt gccgcgcagc gttccggcga gcgctggcat
     2221 tggcagggct tgctggactg gacggcgggc gagttgtact ggcagccgct gtatttcgcc
     2281 aacggtggcc atgtgctcaa tgccgatggc tggctatcgg ccgacacgct ggcggtggag
     2341 cagggcgagc tatcactggc cgagattggc cgcgtcgcgt tcaacggccg cctgagtctg
     2401 gccgacaaaa ctctggcgtc gctgcagctg tcggccaacg acctgcatgc cggaaacgcc
     2461 tacacgctgc tgctcaggcc gttcctgcaa aagggcacgt tcggcgacct ggcaatgtcg
     2521 gggcaggtcg atttccaggc tgatatctcc aaccagcggg tgaaaaattt cgaggtcagc
     2581 ctgcgcgatc tcgacgtgga ggacagccgg cgccgcttcg ccctctacaa gctgaacgcg
     2641 catatcccct ggggttatga cgacaagacc cgggcctggg tgcgctacga cggcggccaa
     2701 ctgctcggtt tgcgcttggg cgcggcgcgg catcaggtca ccctggagcg ctactcgctg
     2761 accgcaccgc acctcgacct gccgctgctg gatggtgtgt tggtaatgaa cgacgtggcg
     2821 gcggcgctgg tcaacgaccg ctggcactgg cgcgttcgtg ccgacatgca gccggtttcc
     2881 atggccgagc ttagccaggc actgggctgg ccgcgcatgg agggcaaggt cgccatgcac
     2941 atccccatga tgtactacac cggcgggcag ttgagcgccg atggcgacct gcgcttcgag
     3001 gtcttcgacg gcacggtggc ggtgaagggg ctggcgatgc aggagccgct ggggttggcg
     3061 ccgcgccttt ctgctgacct ggaaatgcgc aatctcgatc tgggcctgct gaccagcacc
     3121 ttttccttcg gcgctatcca gggaaaattg gacggcgacg tgaagggact ggcgctgtcg
     3181 agctggcagc cggtgaaatt cgatgccagt ttccgtagca gccccggcga ttacccgaaa
     3241 aagatttcgc agcgtgcggt ggagaatatc tcggcgctgg gcggcgctgg tgcggcggtg
     3301 gcgatacagc gcagtttttt gcgctttttc aaggagttca attacgccag catcggcctg
     3361 tcatgcaagc tggataacgg cgtctgcgcc atggatggca tcgcaccggc gcagtccggt
     3421 tatgtcatcg tgaaaggcag cggcatcccg gccatcacgg tgttgggtta caatcgcagc
     3481 gtcaattggg gtgagttgct ggagcgggtg cagcgcatta cgcatggcaa caaaccggtc
     3541 atcaagtaag gggttggagt catgaaaaac tgggtttggc tattgccggc gactttgata
     3601 ttgccggcct gtgtcaccat caacatctac tttccggcag cggcggcgga gaaagcggcc
     3661 gacaagatca tcgacgaagt gtggcagatc aagcccggcg agcagcagcc cgccgaaaag
     3721 cctgcggaaa ccaaatagga gcccaccgtg aacaaacgct taatgactgt atttgtcttg
     3781 ctgctcgcgc tggcggcgcc gctcgcaatg gcggccgcca acctggaaat caatactccg
     3841 gcgattacct ccctgaccgc cagcatgcag gcgcgctaca accagttgtc cccctactac
     3901 gccagcggcg ctatcggcct cacgcgcgac ggtctggtgg cagtgcatga tgccaatacc
     3961 gtgccgctca aggaccggca ggtggtcaat gcgctggtag ccgcggagaa caacgaccgc
     4021 aacgcgctat acaaggagat cgccatcgcc aacggccacc cggagtggga agccgaaatc
     4081 cgcagcactt tcgcccaacg atgggtgcag aaggcgcagg gcggctggtg gtaccagggt
     4141 gcaggcggct gggcgaaaaa gtgacgccaa cgcttgtctt cgacatcgag accatccccg
     4201 ataccgatgg cctgaaaaag ctgctcgacc tgccggccga aacgccggcg gcagacgttg
     4261 ccagtatcgc tttccaccag cgccaccagg ccgggcagtc ggaattcctg ccgctgcacg
     4321 tgcagcgcgt ggcggcgatt tcctgcgccc tgcgcgaagg tgacaatttc cgcgtgtggt
     4381 cgctgggcgg cccggaggat ggcgagggcg cgctgatcca gcgcttcttt gacggcatcg
     4441 acaagtacac gcccacgctg gtgtcgtgga acggcagcgg cttcgacctg ccggtgctgc
     4501 actaccgcgg catgattcac aacgtggtgg cgccgcgcta ctgggacatg ggtgaggacg
     4561 accgcgaatt caagtggaat aactacatca gccgctacca catgcgccat accgacctca
     4621 tggatttact ggccttgtac caggggcgcg gcaacgcgcc gctggaccaa ctggcgcaac
     4681 tgtgtggctt ccccggcaag ctgggcatgg acggttcaaa agtgtggggc gcctggcagg
     4741 ccggcgagat tgaggccatc cgcaactact gcgaaaccga cgtggtgaac acctggctgg
     4801 tgtacctgcg cttccagcag atgcgcgggg cgttgtcgcc ggcggcctac cagcgggaaa
     4861 tcacgctggt gcgcgagacg ttgcaggata cccccggcca gcactggcag gagtttctct
     4921 ccaactgggc ttgagcaggt catgctgatt ctttttattt tctgatactg tccgtttcaa
     4981 ccacatgtca ttcaatcccc ccaccatcgt cgaatcgctc gaccaggaag gccgtggcgt
     5041 cacccatgtc gacggcaagg ccattttcat cgaaggcgcg ctgccaggtg agaaggtgac
     5101 ctattccagc tatcgcaaga aagcttccta cgaattcgcc cacctcgaaa gcctgctcaa
     5161 gcc
//
LOCUS       DAOMFA010000113         5062 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_2179_length_5062_cov_5.294588, whole genome shotgun sequence.
ACCESSION   DAOMFA010000113 DAOMFA010000000
VERSION     DAOMFA010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 5062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5062
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2179_length_5062_cov_5.294588"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            146..604
                     /locus_tag="PKW44_06460"
     CDS             146..604
                     /locus_tag="PKW44_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HQR51261.1"
                     /translation="MGSLSEILGRAQARAREKGLPYAGSLTPEEAYKVLELAPVARLV
                     DVRSRAEWELVGHVPGAAHIEWAFYPGMTPNPDFLAQLTMQVDKEALVMFLCRTGARS
                     HNAAALAAQAGYAESYNILEGFEGETDYVANQRGNLNGWRKAGLPWTNIG"
     gene            608..1408
                     /gene="aroE"
                     /locus_tag="PKW44_06465"
     CDS             608..1408
                     /gene="aroE"
                     /locus_tag="PKW44_06465"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HQR51262.1"
                     /translation="MDRYAVIGNPIEHSKSPQIHAAFARQTGQDIDYGRILAPLGGFH
                     DAVDQFRREGGRGLNVTVPFKEDAYACANQLTQRAQLAGAVNTLNFLPDGTVFGDTTD
                     GAGLVRDITANLGVVLRGKRILLMGAGGAARGVIQPLLDEQPDALVVANRSVGKALAL
                     REPFPQIQAGGYAELAGRSFDIVINATASSLNNDLPPLPTGIFAPGALAYDMMYGRET
                     PFMQFAREQGAVLVADGLGMLVEQAAETFFIWRGVRPQTAPVIAQLRG"
     gene            1405..2097
                     /gene="mtgA"
                     /locus_tag="PKW44_06470"
     CDS             1405..2097
                     /gene="mtgA"
                     /locus_tag="PKW44_06470"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HQR51263.1"
                     /translation="MSLKRLIWRGLLWLLLLIVLYQAWIFLHICWWVNHNPDSSAFME
                     NRLEIMQEKNPDAQLRHKWVPYNRISMYLKRAVIAAEDAKFVDHEGFDWEGMQKAYEK
                     NMKKGKIVAGGSTISQQLAKNLFLSTKRTPWRKLEEAAITLMLEQMMTKRRIFEIYLN
                     VIEWGNGVFGAEAAARHYYKTSAAGLGPEQSARLAAMVPNPRYYDSHRGARGLARKTG
                     IILARMNYAEVP"
     gene            complement(2118..2393)
                     /locus_tag="PKW44_06475"
     CDS             complement(2118..2393)
                     /locus_tag="PKW44_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HQR51264.1"
                     /translation="MNTNSLICRPNCGACCIAPSINSPIPGMPNGKLAGVRCIHLAED
                     YRCALFGLQERPAFCVGLKPAVEMCGETRAQALTWLTRLEQATTPEI"
     gene            complement(2549..3163)
                     /locus_tag="PKW44_06480"
     CDS             complement(2549..3163)
                     /locus_tag="PKW44_06480"
                     /inference="COORDINATES: protein motif:HMM:NF012696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HQR51265.1"
                     /translation="MTSDHQPIYTAEEALNRLKAGNARFVSGHARFPTVQKEVLAELA
                     LGQQPYATILGCSDSRVPPELVFDAGFGELFVVRVAGNVLGPSILGTLQYAGTHLHTP
                     LFVVMGHEGCGAVQAAIASKFHGATQKSRIEVLLENILPALDDLDGTQPAEALLHSAV
                     EANVRHTIRELLATPEAQARLEEGKIRLVGALYDIESGHVRFLE"
     gene            complement(3271..>5062)
                     /gene="thiC"
                     /locus_tag="PKW44_06485"
     CDS             complement(3271..>5062)
                     /gene="thiC"
                     /locus_tag="PKW44_06485"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HQR51266.1"
                     /translation="VYVQGSRPDIRVPFREISLSDTPSAFGAEQNPPVVVYDTAGPYT
                     DPAVQIDIRNGLPAMRAKWIEERNDTETLSGLTSAYGNQRKSDPALAELRFNLTRTPR
                     RAKAGMNVSQMHYARKGIITPEMEFIAIRENQRREGMNELLQTQHAGQNFGASIPKTI
                     TPEFVRDEVARGRAIIPANINHPELEPMIIGRNFLVKVNANIGNSALASSISEEVEKM
                     VWSIRWGGDTVMDLSTGKNIHETREWIIRNSPVPIGTVPIYQALEKVDGKAEDLTWEI
                     FRDTLIEQAEQGVDYFTIHAGIRLQHIPLTAKRMTGIVSRGGSIMAKWCLAHHKESFL
                     YEHFEDICEIMKAYDVSFSLGDGLRPGSIYDANDEAQFAELMTLGELTKIAWKHDVQT
                     MIEGPGHVPMHLIKENMDLQLKHCDEAPFYTLGPLTTDIAPGYDHITSGIGAAMIGWY
                     GCAMLCYVTPKEHLGLPEKEDVRVGIVTYKIAAHAADLAKGHPGAQIRDNALSKARFE
                     FRWEDQFNLGLDPEKAKEFHDETLPQEGAKQAHFCSMCGPHFCSMKITQDVRDYAASQ
                     GLSEQEALQKGMQEKAVEFVKKGAEVYQKA"
BASE COUNT          958 a         1451 c         1572 g         1081 t
ORIGIN      
        1 ttcacacagg cgcttagaaa agaaaacgaa aatgaaatgc accaaaataa ggcgctacat
       61 cattaaatgc accattaagg tgcgctcgtg tgggagagca aataaagact tataatccac
      121 caataatatt gatggaggac atttagtggg tagcttgagt gaaatattgg gtcgcgcgca
      181 agcgcgtgcc cgcgagaaag ggctgccgta tgccgggagc ctgacgcctg aagaagcata
      241 caaggtgctg gaattggctc ccgtggcacg gctggttgat gtacgttccc gcgccgagtg
      301 ggaactggtt gggcatgttc ctggggctgc ccatatcgag tgggcgtttt atcctggaat
      361 gacgcctaat cctgattttc tggcacagct gacgatgcaa gtggataagg aggcgctggt
      421 catgtttctc tgccgcaccg gggcgcgttc gcataacgct gcggctctgg cagcgcaggc
      481 tgggtatgcg gaaagctaca acattcttga aggtttcgag ggtgagactg attatgtggc
      541 caaccagcgt ggcaacctca atggctggcg caaggccgga cttccatgga ccaacatcgg
      601 ctgaagcatg gaccgctacg ccgtcatcgg caaccccatt gagcatagca agtcaccgca
      661 gattcatgct gcgtttgctc ggcagacggg gcaggacatc gactatggtc gtatcctggc
      721 accactcggt ggctttcacg acgccgtaga ccagtttcgc agagagggtg ggcgtggtct
      781 caacgtcacg gtaccgttca aggaagacgc ctatgcctgc gccaatcagt tgacgcagcg
      841 cgcgcaactg gccggcgcgg tcaacaccct gaattttctg cctgacggca cggtgttcgg
      901 tgataccacc gacggtgctg ggctggtccg tgacatcacc gccaatctcg gcgttgtcct
      961 gcgcggcaag cgcatcctgc tgatgggggc gggaggagcc gcgcgcggcg tcatccagcc
     1021 gctgctggac gagcagcctg acgctctggt ggtagccaac cgcagcgtgg ggaaagccct
     1081 cgctttgcgt gaacctttcc cgcaaatcca ggctggcggc tatgccgagc tggccggacg
     1141 aagcttcgat atcgtcatca acgctactgc cagcagcctc aacaacgacc tgccgccgct
     1201 acctaccggc atcttcgccc ccggcgcact ggcctacgac atgatgtatg gtcgcgagac
     1261 gccattcatg cagttcgctc gcgagcaggg cgcggtgctt gtagctgatg gactcggcat
     1321 gctggtggaa caggcggcgg aaaccttctt catctggcgc ggcgtgcgtc cacagacggc
     1381 gccggtgatc gcccaactac gcggatgagc ttgaaacgcc ttatctggcg cggcctgctc
     1441 tggctgctcc tgctgatcgt gctctaccag gcgtggatat tcctccacat ctgctggtgg
     1501 gtaaaccaca accctgattc cagcgccttc atggaaaacc ggctggaaat catgcaggaa
     1561 aagaatccag acgcccagct gcgccacaag tgggtgccct acaaccgtat ctccatgtat
     1621 ctcaaacgtg ccgtcatcgc cgccgaggac gccaaattcg tcgaccatga aggctttgac
     1681 tgggaaggta tgcagaaagc atacgaaaaa aacatgaaga aaggcaagat cgtcgccggc
     1741 ggctccacca tcagccagca gctcgccaag aacctgttcc tctccaccaa gcgcacgcca
     1801 tggcgcaagc tggaagaagc ggcaatcacg ctgatgctgg aacagatgat gacgaagcgc
     1861 cgtattttcg agatttatct caacgtcatc gagtggggca acggcgtgtt cggcgccgag
     1921 gctgccgcgc gccactacta caaaacctct gctgctggtc tgggtccgga gcagtcggcc
     1981 agattggctg ccatggtgcc caacccgcgt tactacgaca gtcaccgcgg cgcgcgtggc
     2041 ctggcgcgca agaccggtat catactggca cgtatgaatt acgccgaagt gccttgattt
     2101 ctggtgatgg cgcctgctta tatctcaggc gtcgtggcct gttccaggcg ggtcaaccac
     2161 gtcagcgcct gtgcgcgggt ttctccgcac atttcaactg cgggctttag cccgacgcag
     2221 aaagccgggc gctcctgtag tccgaacagc gcgcaacgat aatcttctgc gaggtggatg
     2281 caacgcaccc cggcaagctt gccgttgggc atgccgggga tgggggagtt gatcgagggg
     2341 gcaatacaac aagcgccgca gttggggcgg caaatcaggg aatttgtatt catgctggcg
     2401 ttcgacaatt atcgtgtccg atcccattct ggtgacttgc tgcaccgaat ttctatggct
     2461 ggcgtggcgt gtagcgaagc ctaaccattt tagtaaccgt tgggcttcac ttcgttcagc
     2521 gccaacctac aagcccacga tagtggcgct attccagaaa cctgacgtgc cccgactcga
     2581 tgtcgtatag cgcgccgacc agcctgattt ttccctcctc aaggcgggcc tgtgcttcag
     2641 gcgttgcgag tagttcgcgt attgtatgcc ggacattggc ttcgaccgcc gaatggagca
     2701 gcgcctcggc aggctgtgtg ccatcaaggt catccagcgc cggcaggatg ttctctagca
     2761 gcacctcgat ccggcttttt tgcgtggcgc cgtggaattt cgaggcaatc gcggcctgca
     2821 ccgccccgca tccctcgtgg cccatgacga cgaaaagcgg tgtgtggaga tgcgtgcctg
     2881 catattgcag cgtacccagg atggaggggc cgagcacatt gcccgccacc ctcacgacaa
     2941 acagctcgcc gaacccggcg tcaaagacca gctccggtgg cacacggcta tcgctgcaac
     3001 ccagtatcgt ggcgtagggc tgctggccaa gggccagctc cgccagaacc tccttctgca
     3061 ccgtagggaa gcgcgcatgg ccgctgacga agcgcgcgtt gcccgctttc aggcgattga
     3121 gggcttcttc cgccgtgtaa atgggttgat ggtcagatgt catgattcgc gatatgcatt
     3181 atcaaagact ggtgtgctgt cgtagggcgc acccgcctgc agtgcgccgc atgttcgtca
     3241 tgcttatctt gcttgcatgg cgaaaaacca tcaagccttc tgataaacct cagccccctt
     3301 cttgacgaat tccaccgcct tttcctgcat gcccttctgt agcgcttcct gttcggaaag
     3361 cccctggctg gcggcatagt cgcgcacgtc ctgggtaatt ttcatggagc agaagtgggg
     3421 gccacacatg gagcagaagt gggcctgctt ggcgccttcc tgcggcaggg tttcgtcgtg
     3481 gaattccttg gctttttccg ggtcgaggcc gaggttgaac tggtcttccc agcggaattc
     3541 aaagcgggcc ttggagagcg cgttgtcgcg gatttgcgcg ccggggtggc ctttggcgag
     3601 gtcggcggcg tgggcggcga tcttgtaggt gacgataccg acgcgcacgt cttctttctc
     3661 cggcaggccc agatgttctt tcggagtgac gtagcacagc atggcgcagc cgtaccagcc
     3721 aatcatggcg gcgccgatgc cggaggtgat gtggtcgtaa cccggggcga tatcggtggt
     3781 gagcgggccc aaggtgtaga acggcgcttc gtcgcaatgt ttcagctgca ggtccatgtt
     3841 ctctttaatg aggtgcatgg gcacgtggcc ggggccttcg atcatggtct gcacgtcgtg
     3901 cttccaggcg atcttggtca gttcgcccag cgtcatcagc tcggcgaact gcgcttcgtc
     3961 gttggcgtcg taaatcgaac ccggacgcag gccgtcgccc agactgaagc tgacgtcgta
     4021 ggccttcatg atttcgcaga tgtcctcgaa atgttcgtag aggaaggatt ccttgtggtg
     4081 cgccagacac cacttggcca taatggagcc gccgcgcgag acgatgccgg tcatgcgctt
     4141 ggcggtgagc gggatgtgct gcaagcggat gccggcgtgg atggtgaagt agtccacgcc
     4201 ctgttcggct tgttcgatca gggtgtcgcg gaagatttcc caggtcaggt cctcggcctt
     4261 gccgtccacc ttttccagcg cttgatagat cgggactgtt ccaatcggta caggggagtt
     4321 gcggatgatc cactcgcgcg tctcgtggat gttcttgccg gtggacaggt ccatcacggt
     4381 gtcgccgccc cagcggatgc tccacaccat tttttccact tcctcggaaa tgctggaagc
     4441 cagcgcggag ttgccgatgt tggcgttgac cttgaccagg aaattgcggc cgatgatcat
     4501 cggctccagc tccgggtggt tgatgttggc ggggatgatg gcgcggccac gcgccacttc
     4561 gtcgcgcacg aattccggcg tgatggtttt cgggatggac gcgccgaaat tctgcccggc
     4621 gtgctgggtt tgcagcagtt cgttcatgcc ctcgcggcgc tggttctcac ggatggcgat
     4681 gaattccatt tccggcgtga tgatgccttt gcgcgcatag tgcatttgcg atacgttcat
     4741 gccggctttg gcgcggcgcg gcgtgcgcgt caggttgaag cgcaactcgg ccagtgccgg
     4801 atcggacttg cgctggttgc cgtaggcgga agtcagtccg ctcagcgtct cggtatcgtt
     4861 acgctcctcg atccacttgg cgcgcatggc cggaaggccg ttgcggatgt cgatctggac
     4921 ggccggatcg gtatacgggc cggccgtgtc gtataccacc accggcgggt tctgttccgc
     4981 accaaaggcg gatggcgtgt cggacaggct gatttcgcgg aatggcacgc gaatgtccgg
     5041 gcggctgccc tgcacataga cc
//
LOCUS       DAOMFA010000114         5056 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_2183_length_5056_cov_4.842032, whole genome shotgun sequence.
ACCESSION   DAOMFA010000114 DAOMFA010000000
VERSION     DAOMFA010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 5056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5056
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2183_length_5056_cov_4.842032"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(40..585)
                     /locus_tag="PKW44_06490"
     CDS             complement(40..585)
                     /locus_tag="PKW44_06490"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018076928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HQR51267.1"
                     /translation="MTDLTETELSSETVFQGSLLHVKRDTVRLPNGSETGREYITHPG
                     AVLVVPVLDDGRLVFERQFRYPLRKSFIELPAGKIDPSEDPLETGRRELLEETGYTAR
                     DWRYLAALHPCIGYSDEIIHIYLASGLQAGEHSRDHDESLEVFTLTLDEAMQALRRGE
                     ITDGKTMIALFWAEKNLAGSW"
     gene            complement(743..1033)
                     /locus_tag="PKW44_06495"
     CDS             complement(743..1033)
                     /locus_tag="PKW44_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2818 family protein"
                     /protein_id="HQR51268.1"
                     /translation="MSTTHLILILLGLLAANLPWFSERLFYLVPLKHKHGGWRLLELV
                     VLYFLVGGLALLAEHNAAGQIARQHWEFYATTACLFLVFAFPGFVYRYLWRK"
     gene            complement(1033..2490)
                     /gene="nuoN"
                     /locus_tag="PKW44_06500"
     CDS             complement(1033..2490)
                     /gene="nuoN"
                     /locus_tag="PKW44_06500"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HQR51269.1"
                     /translation="MNSAIVSDLYAALPEIFVLVMAMAVLLASVFLTERNRYVAYILT
                     QITLLVAAFITVSTHTSGVGYSFNGMFVDDPMADVLKMMIYLATSVMLIYSRSYIALR
                     GMYRGEFFALVLFAMLGMMVMVSGHNFLTLYMGLELLSLSLYTLVALDRDNARATEAA
                     MKYFVLGALASGMLLYGMSMIYGATGSLDISQISSSLMNGVPDHAVLILGLVFIVAGL
                     AFKLGAVPFQMWVPDVYQGAPTAITLLIGSAPKLAGFAIVIRLLVQGLEPLVIDWRGM
                     LVLMAVLSIVIGNVTAIAQTNIKRMLAYSTISHVGFLLLGFLSFNPLNYNGPASAMFY
                     ISVYVLMTLGAFGVILLLSRKGFEAENLDDFKGLSQRSPWYAFLMLLLMFSMAGVPPT
                     LGFYAKFAVLQAALQAGYVWLVVYAVLMAVIGAFYYLRVVKLMYFDEPLDHTPIAPQF
                     DVSIVLGVNGLAVLVLGLMPQRLMELCAYAIAHSL"
     gene            complement(2491..3984)
                     /locus_tag="PKW44_06505"
     CDS             complement(2491..3984)
                     /locus_tag="PKW44_06505"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020182345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HQR51270.1"
                     /translation="MFESNILSLSIWVPILAGILVLLTGGDRNATLARWLALAGSIAG
                     FVVTLPLWTRFDRADGGFQFQEGWGWIPSFNIHYHLGVDGIAVPLILLTSFTTVIVVL
                     AGWEVIQKRVAQYMAAFLIMSGLMIGVFSALDGILFYVFWEAMLIPMFLVIGVWGGAN
                     RVYATIKFFLYTLLGSLLMLVAFIYLFHQTNSFEIVDYYALPLPLTAQVLIFIAFFMA
                     FAVKIPMWPVHTWLPDAHVEAPTGGSVVLAAIMLKLGGYGFLRYTMPIAPDAAHYLSG
                     FMITLSLIAVVYIAFVALVQQDMKKLIAYSSISHMGFVTLGMFIFNPLGMEGSMVQMI
                     SHGFVSGALFLCVGVLYDRMHSRQIADYGGVVNTMPTFTALAVLFAMANAGLPGTSGF
                     VGEFMVILGALQENFWYAFLAATTLIFGAAYTLWMVKRVYFGELTNPHVAALKDVNKR
                     ELLILGVLALLVIGFGVYPQPITEMTHATVNQLLSHLANSKLPGGTF"
     gene            complement(3985..>5056)
                     /locus_tag="PKW44_06510"
     CDS             complement(3985..>5056)
                     /locus_tag="PKW44_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HQR51271.1"
                     /translation="NDINRVVAYSTLAQLGYMTVALGASAYSVAIFHLMTHAFFKALL
                     FLAAGSVIMGMHHDQDIRNMGGLRKYMPITWVTSLIGSLALIGTPFFSGFYSKDSIIE
                     AAELSHIPGSGFAYFAVLAGVFVTAFYSFRMYFLVFHGKERWLAAGHGHGSHDDHHHG
                     LGPHDKPHESPWVVTVPLIALAIPSVVIGYLTIGPMLFGGFFSGAIFYNLAAHPTMRE
                     LAHHFHGAAAMGLHAFATLPFLLAAAGVGLSWFLYLKRPDIPEAIKQRFSLIYRILDN
                     KYGFDAFNEKFFAGGARWIGGKLWQIGDVRLIDGFFVNGTARLIGRISAVVRHLQSGL
                     IYHYAFAMIIGVFVLLTFFMKN"
BASE COUNT         1163 a         1473 c         1588 g          832 t
ORIGIN      
        1 agcgggccaa cgccttgagc atttcgtcgc agtggagttt caccagcttc cggcgaggtt
       61 cttctctgcc cagaacaggg caatcatggt tttaccatcg gtgatttcac cgcggcgcag
      121 cgcttgcatc gcctcgtcca gcgtaagggt gaacacctcc aggctttcgt cgtggtcgcg
      181 gctgtgctca cctgcctgca gtccgctagc caggtagata tgaataattt cgtcggaata
      241 gccgatgcag ggatgtagcg ccgccagata gcgccagtcg cgggcggtgt agccggtttc
      301 ttccagcagt tcgcgcctgc cggtttccag cgggtcctcg ctggggtcga tcttgccggc
      361 ggggagctcg atgaagctct tacgcagcgg gtagcggaac tggcgctcga acaccaggcg
      421 cccgtcatcc agtactggta ccaccaacac ggcgccgggg tgagttatgt attcacgccc
      481 ggtctcgctg ccattgggca ggcgcaccgt gtcgcgcttg acgtgcagca ggctgccctg
      541 aaaaaccgtt tcggaagaaa gttcggtttc ggttaaatcc gtcatgaggt ttcctggtgc
      601 gctgtataac cgtagcgagc ggcctcaagt gcagggagcc gcgtagcgaa cgacgagaca
      661 tgccgtgcct ggcaggcaag gagtgagcga gtacgcgacg ccgcaattga ggtgcgcagt
      721 aggttaggca gtgtgccagc gttcatttac gccacaggta gcggtatacg aagccgggaa
      781 atgcaaacac caggaacagg caggcggtgg tggcatagaa ctcccaatgc tggcgggcga
      841 tctggccggc ggcattgtgt tcggccagca gcgccagtcc gccgacgagg aaatacagca
      901 ccaccagttc cagcagccgc cagccaccat gtttgtgttt cagcggcacc aggtagaaca
      961 agcgctcgga gaaccacggc agatttgcag ccagcaggcc aagcaaaatc aggatcaggt
     1021 gggtggtgct catcacaggc tatgggcgat agcgtaggcg cacagctcca tcaggcgctg
     1081 cggcatcagg cccagcacca gcacggcaag gccgttgacg ccaagcacga tgctgacgtc
     1141 gaactgcggc gcgatgggag tgtgatccag cggttcgtcg aaatacatca gcttgaccac
     1201 gcgcaggtag tagaaagcgc cgatcactgc catcagcacg gcgtacacca ccagccagac
     1261 atagccggcc tgcagcgccg cctgcagtac cgcgaacttg gcatagaagc ccagcgtggg
     1321 cggcacgccc gccatggaga acatcaacag cagcatgaga aaggcatacc acgggctgcg
     1381 ctggctcaag cccttgaagt cgtccaggtt ttccgcctcg aagcccttgc gtgacagcag
     1441 caggatgacg ccgaaggcgc ccagcgtcat cagcacatag accgagatgt agaacatggc
     1501 cgaagccggg ccgttgtagt tgagcggatt gaaactgagg aaaccgagca gcaggaaacc
     1561 gacatgcgaa atggtggaat aagccagcat gcgcttgata ttggtctgcg cgatggcggt
     1621 gacgttgccg ataacgatgg acagcaccgc catcagcacc agcatgccgc gccagtcgat
     1681 caccagcggc tccagcccct gcaccagcag gcggatgacg atggcgaagc cggccagctt
     1741 tggcgcagag ccgatcagca gggtaatcgc cgtcggcgcg ccttgataga catcgggcac
     1801 ccacatctgg aacggcaccg cccccagttt gaacgccagc ccggccacga tgaacaccag
     1861 tcccaggatg agtacggcat gatccggcac gccattcatc agggaggaac tgatctggct
     1921 gatatccagc gaaccggtgg cgccatagat catggacatg ccatacagca gcatgccgga
     1981 agccagtgcc cccagcacga aatacttcat cgccgcctcg gtggcgcggg cattgtcgcg
     2041 gtccagtgct accagcgtat acaggctgag ggagagcaac tccagcccca tgtacagggt
     2101 caggaagttg tggccggaca ccatcaccat catgcccagc atggcgaaca gcaccagcgc
     2161 gaagaattcg ccgcggtaca tgccgcgcag ggcaatgtag ctgcgcgaat agatcagcat
     2221 caccgaggtg gcgaggtaga tcatcatctt cagcacgtcc gccatcgggt cgtcgacgaa
     2281 catgccgttg aaagagtagc cgaccccgga agtatgggtg gagaccgtga tgaatgcggc
     2341 gaccagcaag gtaatctgcg tcaggatgta agcgacgtag cggttgcgct cggtcaggaa
     2401 cacgctggcc agcaacaccg ccatggccat gacgagcacg aaaatttccg gcagtgcggc
     2461 gtagagatca ctcacgattg cgctgttcat ttaaaaggtc cctcccggca gcttgctatt
     2521 tgccaggtgc gacagcaact ggttgacggt ggcatgggtc atttcggtaa taggctgcgg
     2581 atagacgccg aagccgatca ccagcaaggc cagcacgccc aggatcagca gttcgcgctt
     2641 gttcacgtct ttcaacgcgg cgacatgcgg gttggtcagt tcgccgaagt acacccgctt
     2701 gaccatccac aaggtgtagg cggcgccgaa gatcagcgtg gtggcggcga ggaaggcata
     2761 ccagaagttt tcctgcagcg cgccgaggat gaccataaac tcgccgacga agccggaagt
     2821 gccaggcagg ccggcattgg ccatggcgaa cagcactgcc agcgcggtaa aggtcggcat
     2881 ggtgttgacc acgccaccgt aatcggcgat ctggcgcgaa tgcatgcggt cgtacagcac
     2941 gccgacgcac aggaacaagg cgccggagac gaagccgtgc gaaatcatct gcaccatgct
     3001 gccttccatg ccaagcgggt tgaagatgaa catgccgagc gtgacgaagc ccatgtgcga
     3061 gatggacgaa taggcgatca gcttcttcat gtcctgctgc accagcgcta cgaaagcaat
     3121 gtagaccacg gcgatcagcg acagggtgat catgaatccg gacaggtaat gcgccgcgtc
     3181 tggcgcgatc ggcatggtgt agcgtaggaa accatagcca cccagcttca gcatgatggc
     3241 cgccagcacc accgatccgc cagtcggcgc ctccacgtgc gcatctggaa gccaagtgtg
     3301 caccggccac atcgggatct tgaccgcgaa cgccatgaag aaggcgatga agatcagcac
     3361 ctgtgccgtc agcggcagcg gcagcgcgta gtagtccacg atctcgaagc tgttggtctg
     3421 gtggaacagg tagatgaaag ccaccagcat cagcagcgag ccgagcaggg tatagaggaa
     3481 gaacttgatg gtggcataca cccggtttgc cccgccccac accccgatca ccaggaacat
     3541 cgggatcagc atggcttccc agaacacgta gaacaggatg ccatccagcg ccgagaacac
     3601 gccgatcatc aggccggaca tgatgaggaa ggctgccatg tactgcgcca cgcgcttctg
     3661 gatgacttcc caaccggcca gcaccacgat cacggtggtg aaactggtga gcaggatcag
     3721 cggcaccgcg atgccgtcca cgcccaggtg gtaatggatg ttgaagctgg gaatccagcc
     3781 ccagccctcc tggaactgga aaccgccgtc ggcgcggtca aaacgtgtcc acaaggggag
     3841 cgtgacgacg aagccggcga tgctgccggc cagcgccagc cagcgtgcca aggtggcgtt
     3901 gcggtcaccg ccggtaagca acaccaggat gcctgccagg atgggcaccc agatggaaag
     3961 gctgaggatg ttggactcga acatttaatt cttcatgaag aaggtgagca ggacgaacac
     4021 gccgataatc atggcgaagg catagtggta gatcaggccg gattgcagat ggcggaccac
     4081 cgccgagatg cgtccgatca ggcgcgcggt gccgttgacg aagaagccgt cgatcagcct
     4141 gacatcgccg atctgccaca gcttgccgcc gatccagcgc gcaccgccgg caaagaactt
     4201 ttcgttgaaa gcgtcgaagc cgtatttgtt gtctaggatg cggtagatca gcgagaaacg
     4261 ctgcttgatc gcttccggga tgtccggccg cttcaggtag aggaaccagg acagccccac
     4321 cccagcggcc gccagcagga acggcagcgt agcaaaggca tgcagaccca tcgctgcggc
     4381 accgtggaaa tgatgcgcca gctcacgcat ggtcggatgc gcggccaggt tgtagaaaat
     4441 cgccccgctg aagaaacctc cgaacagcat gggcccgatg gtcaggtagc cgatgacaac
     4501 cgatggaatc gccagtgcaa tcagcggcac cgtcaccacc cagggcgatt cgtgcggctt
     4561 gtcgtgcggg ccgagaccat ggtgatggtc gtcatgactg ccatggccat gtccggccgc
     4621 cagccagcgc tccttgccgt ggaacaccag gaaatacatg cggaacgagt agaacgcggt
     4681 gacgaacacg cccgccagca cggcgaaata ggcgaagccg ctgccgggga tgtgcgacag
     4741 ttcggccgcc tcgatgatgg agtccttgga atagaagccg gagaagaacg gcgtaccgat
     4801 cagcgccagc gacccgatca gtgaagtgac ccaggtaatc ggcatatatt tacgcagccc
     4861 gcccatgttg cggatgtcct ggtcgtggtg catgcccatg atgaccgaac cggcggcgag
     4921 gaacagcagc gccttgaaga aggcatgcgt catcaggtgg aagattgcca ccgagtaggc
     4981 tgaggcgcca agggcgacgg tcatgtagcc gagttgtgcc agggtcgagt aggcaacgac
     5041 gcgattgatg tcgtta
//
LOCUS       DAOMFA010000115         5014 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_2209_length_5014_cov_4.961081, whole genome shotgun sequence.
ACCESSION   DAOMFA010000115 DAOMFA010000000
VERSION     DAOMFA010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 5014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5014
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2209_length_5014_cov_4.961081"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..525)
                     /locus_tag="PKW44_06515"
     CDS             complement(<1..525)
                     /locus_tag="PKW44_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HQR51272.1"
                     /translation="MHTAFISHPDTLLHVMDGNHPESPARISAIKDRLMAARLYDHLQ
                     IHDAPLATDAQLARVHAPDYIRHIRAVAPQAGIVHLDQDTAMGPMTLSAVLHAAGAVV
                     LAVDLVMSGEAQNAFCCVRPPGHHAGHASAAGFCIFNNVAVGVAHAMAKHGIERAAII
                     DFDVHHGDGTEDIFR"
     gene            complement(536..1411)
                     /gene="rsgA"
                     /locus_tag="PKW44_06520"
     CDS             complement(536..1411)
                     /gene="rsgA"
                     /locus_tag="PKW44_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HQR51273.1"
                     /translation="MAINALQSGLVIAAFGKRYDIELANGRQIGCVTRGKKSDLACGD
                     RVEIKLTSGNEGVIENTIPRSSLLYRSNAFRSKLLAANVTQIIIVLAAVPSFYEDLLN
                     RCLIAAEAAGIRALILLNKSDLPETATALQRLQLYLSLGYAVQPLSAKQDALPLRHWL
                     QGQASVLVGKSGMGKSTLVNALLPEVNSRTQEISAVLDSGKHTTTSARLYHLDADSHL
                     IDSPGLQEFGLSHLSTDQMEHAFIEFRPYLGKCRFNNCRHLKEPGCAILGAVADGKIS
                     AIRLQCYQKLLAELS"
     gene            complement(1412..1726)
                     /locus_tag="PKW44_06525"
     CDS             complement(1412..1726)
                     /locus_tag="PKW44_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HQR51274.1"
                     /translation="MSRQLVHLTLLALAATAAHADPFAAGDPKVGRQMVEKNCIACHA
                     SRFGGDGSAIYTREDRKIKDAKGLIQQIRTCNTNLGLKWFEDEELNVASYLNKSYYKF
                     DK"
     gene            complement(1738..2982)
                     /locus_tag="PKW44_06530"
     CDS             complement(1738..2982)
                     /locus_tag="PKW44_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HQR51275.1"
                     /translation="MMTTFTILFVGLLLLTTFVRLWLGRRHVAFIQAHRAQVPAAFSA
                     TINLDAHQKAADYSSAKTRLVMLEGVVQALLLLALTLGGILQWLDQGWLAVMPESTLL
                     RGALVILSAFVITSLVDIPFDYYKAFVIDQRFGFNKMSRAMFFTDLAKHALIGLALGA
                     PLLFTALWLMQGAGNWWWLYLWMVWSVFNLLMLAIYPTFIAPLFNKFKPLENEALKTR
                     IEALLAKCGFKSQGLFVMDGSTRSSHGNAYFTGFGANKRVVFFDTLLERLDADEIEAV
                     LAHELGHFKCHHVIKRISLLFAVSFAGLALLGWLVNQPWFYTGLGVATPSNHMALLLF
                     LLVSPVFTFLLRPLFSGYARNNEFEADAYAAAHASAAHLVASLVKLYRDNASTLTPDP
                     LHSAFYDSHPPASLRIARLEAS"
     gene            3039..3590
                     /gene="orn"
                     /locus_tag="PKW44_06535"
     CDS             3039..3590
                     /gene="orn"
                     /locus_tag="PKW44_06535"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-exoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HQR51276.1"
                     /translation="MAQDNNNLVWIDLEMSGLLPDSDRILEMAVVVTDSQLDILGESP
                     VLVVHQADAVLDGMDAWNKGTHGKSGLIDKVKASTLNEAEAEAQMIEFLKQYVPASKS
                     PMCGNSICQDRRFLARYMPKLEAYFHYRNLDVSVFKELARRWRPKLYDGFKKASKHTA
                     LADIHESIDELKYYREHFIRLEE"
     gene            complement(3774..>5014)
                     /gene="glgA"
                     /locus_tag="PKW44_06540"
     CDS             complement(3774..>5014)
                     /gene="glgA"
                     /locus_tag="PKW44_06540"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HQR51277.1"
                     /translation="LMIGEMPDTGVPVIVIACPHLYERDSGPYLDTRGYEWLDNPLRF
                     GILSYVAALLGSKQSVLADWIPDVVHFNDWQGGLTPAFMRYAGKPSAKSVLGIHNLAF
                     QGNCAEDWVARLGLPLEAYHIDGLEYYGKLSFLKAGVYYADSITTVSPTYAREIQTPE
                     FGFGMQGLLSTRSKDIHGILNGIDTHEWNPAADHHLPHAYDGAHLENKQLNKLALQES
                     LGLAVDVSAPLLGVVSRLTHQKGLDLFAECAPTLLEQGCQISLLGGGEPSLEHAFGQL
                     ALQHPNQVSVNIGYNEALSHRIMAGADMFIMPSRFEPCGLNQMYGLRYGTPPVVRRTG
                     GLADSVCDTTPATLADGSATGFVFDTADAATLLETIQRALSSYHNPAQWKQIQSNGMA
                     RDLGWDASARAYRDIYRSLL"
BASE COUNT         1012 a         1383 c         1625 g          994 t
ORIGIN      
        1 gcggaaaata tcctcggtgc cgtcgccgtg gtgcacgtca aaatcgatga tggctgcacg
       61 ttcgatgccg tgcttcgcca tggcatgtgc cacgcctacc gcgacattgt tgaagataca
      121 gaagcccgcg gcgctggcat gccctgcgtg atgtcccggc gggcggacgc agcagaaggc
      181 gttctgcgcc tcgcccgaca tcactaggtc taccgcgagc acgacggcgc cagccgcatg
      241 caacacagca gaaagcgtca tcggccccat ggcggtatcc tggtccaggt gcacgatgcc
      301 ggcttgcggc gcgacggcgc ggatgtgccg gatgtagtca ggcgcatgca cacgcgccag
      361 ttgagcatcg gtggccagcg gcgcgtcgtg gatctgcagg tggtcataca ggcgtgccgc
      421 catcagcctg tccttgattg ccgagatgcg cgccggcgat tccggatgat tgccgtccat
      481 cacgtgcagc agggtgtcgg ggtggctgat gaaagcggtg tgcatctaag gggtctcagg
      541 aaagttctgc cagcagtttc tgatagcact gtagccggat tgcgctgatt tttccatctg
      601 ccacggcgcc gaggatggca caaccaggtt ccttgaggtg gcggcaattg ttgaagcggc
      661 atttgccgag atacggccgg aactcgatga aggcgtgctc catctgatcc gtgctcaggt
      721 ggctgaggcc gaactcctgc aagccggggg aatcgatcag gtggctgtcg gcgtccaggt
      781 ggtaaagtct tgccgaggtg gtggtgtgct tgccgctgtc cagcacggcg gagatttcct
      841 gcgtgcgcga gttgacctct ggcagcagcg cattgaccag cgtggacttg cccatgccgg
      901 attttcccac cagcacgctg gcctggcctt gcagccagtg gcgcaagggg agggcgtcct
      961 gtttcgccga gagcggctgc accgcgtagc ccagcgacag gtaaagctga agccgttgca
     1021 gcgccgtggc ggtttccggc aggtcgctct tgttgagcag gatcagggcg cggatgccag
     1081 ccgcctcggc ggcgatcagg cagcgattga gcaggtcttc gtaaaagctg ggcacggcgg
     1141 ccagcacgat gatgatctgg gtgacgttgg ccgccagcag cttgctgcgg aaggcgttgc
     1201 tgcggtagag caggctgctg cgcggaatgg tattctcgat gacgccttcg ttgccggagg
     1261 ttagcttgat ttcgacgcgg tcgccgcagg ccaggtcgct tttcttgccg cgcgtcacgc
     1321 agccgatttg ccgcccattt gccaactcaa tgtcatagcg cttgccaaag gcggcgatga
     1381 ccaagccaga ttgcaacgcg ttgatagcca actacttgtc gaacttgtag taactcttgt
     1441 tcaggtagct ggccacattc agctcctcgt cttcgaacca cttgaggccc aggttggtgt
     1501 tgcaggtgcg aatctgttgg atcaggcctt ttgcatcctt gattttgcgg tcttcacggg
     1561 tgtagatggc ggagccgtcg ccgccgaagc gcgaggcgtg gcaggcgatg cagttctttt
     1621 ccaccatctg cctgccgacc ttcggatcgc cggcggcaaa cgggtcggca tgggcggcgg
     1681 tggcggcaag ggcgagcagg gtaaggtgaa cgagttgtct ggacatgaga ttctccttca
     1741 agaggcttcc agcctggcga tacggaggct ggctggcgga tgcgaatcgt agaacgccga
     1801 atgcaacggg tcgggggtga gggtggaggc gttgtcgcgg tagagcttga ccagcgaggc
     1861 gaccaggtgc gccgcgctgg catgggcggc ggcgtaggcg tcggcctcga actcgttgtt
     1921 gcgcgcatag ccggaaaaca gcgggcgcag caaaaaggtg aacaccgggc ttaccagcag
     1981 gaacagcagc agcgccatgt ggttgctggg cgtggcgacg ccaaggccgg tgtaaaacca
     2041 tggctggttg accagccagc cgagcagcgc cagtccggca aagctgacgg caaatagcag
     2101 gctgatgcgc ttgatgacgt ggtggcactt gaaatgcccc agttcatgcg ccagcaccgc
     2161 ctcgatctcg tccgcatcca gacgttccag cagggtatcg aagaacacca cgcgcttgtt
     2221 ggcgccgaag ccggtgaaat aggcgtttcc gtggctgctg cgggtggaac cgtccatcac
     2281 gaacagcccc tgcgacttga agccgcattt ggccagcagc gcctcgatgc gggttttcag
     2341 cgcttcgttt tccaatggct tgaacttgtt gaacagcggc gcgataaagg tggggtagat
     2401 ggccagcatc agcaggttga acaccgacca gaccatccac aaatacagcc accaccaatt
     2461 gccagccccc tgcatcagcc acagcgcggt gaacagcagc ggcgcgccca gcgccaggcc
     2521 gatcagggcg tgcttggcga ggtcggtgaa gaacatggcg cgcgacatct tgttgaagcc
     2581 gaagcgctga tcgatgacga aggctttgta ataatcgaac gggatgtcca ccaggctggt
     2641 gatgacgaag gcggacagga tgaccagtgc gccgcgcagc aaggtggatt ccggcatgac
     2701 ggccagccag ccctgatcca gccactgaag gatgccgccc agagtcagtg ccagcagcag
     2761 cagggcctgc accacgcctt ccagcatgac cagccgggtc tttgcagaag aatagtcggc
     2821 cgctttctgg tgggcgtcca ggttgatggt ggcgctgaag gcggccggta cctgtgcccg
     2881 gtgtgcctgt atgaaggcga catggcggcg ccccagccac agacgaacga aggtggtcaa
     2941 cagcaacagg ccgacaaaca ggatagtgaa ggtggtcatg atcgattttc ggttaaactc
     3001 aacgcttttt tgaaacgcga atagcaggaa ggttcattat ggcacaggac aacaataatc
     3061 ttgtctggat cgacttggaa atgagcgggt tgctgcccga ttcggaccgg atactcgaaa
     3121 tggccgtggt agtcaccgat tcgcagctcg atatcctggg cgaatcgccg gtgctggtgg
     3181 tgcaccaggc cgatgcggtg ctggacggca tggatgcttg gaacaagggt acgcatggca
     3241 aatccgggct gatcgacaag gtcaaggctt cgaccctgaa cgaagccgaa gccgaagcgc
     3301 agatgatcga atttctaaag caatatgtgc cggccagcaa atcgccgatg tgcggcaatt
     3361 ccatttgcca ggatcggcgg tttctggcgc gctatatgcc gaagctggaa gcctacttcc
     3421 actaccgcaa cctggatgtc agcgtattca aggagctggc gcggcgctgg cgccccaagt
     3481 tgtatgatgg cttcaagaaa gccagcaaac atacggcgct ggcggatatt catgaatcca
     3541 tcgacgagct gaaatactac cgcgagcatt tcatccggtt agaggaatag agtctggaca
     3601 aaaaagaggg cgccaagggc gcccgaaggg aggagattta agcttggaga agctcgctta
     3661 ttcttaagca atatgtgtgc cataagctaa ttttttctgc ttttgtcagc cgggtttgac
     3721 gtgaaaagaa gatggcgcac cggaccggcg cgccatctta aagctgcgat tggttatagc
     3781 agtgagcggt atatgtcgcg ataggcacgt gcgctggcgt cccagcccag gtcccgcgcc
     3841 atgccgttgc tctggatttg cttccattgc gccgggttgt ggtagctgga cagcgcgcgc
     3901 tggatggttt caagcaaggt ggcggcatcc gcggtatcga atacgaagcc ggtcgcagaa
     3961 ccgtctgcaa gcgtggccgg ggtagtgtcg catactgagt ccgccaatcc gccagtacgg
     4021 cgtactacag gcggcgtccc atagcgcaag ccatacatct gattgagacc gcagggctcg
     4081 aagcgcgaag gcatgatgaa catgtcggcg cctgccatga tccggtgcga aagcgcttcg
     4141 ttgtagccga tattgacgct gacctggttc ggatgctgca gcgccagttg cccgaatgcg
     4201 tgttccaggg acggctcgcc gccgcccaga agggagatct gacagccttg ctccagcagg
     4261 gtcggggcgc attctgcaaa caagtccagc cctttctggt gcgtaagccg gctgaccacg
     4321 cccagcaatg gggcgctgac atctaccgcc agccccagac tttcttgcaa ggccagtttg
     4381 ttcaattgct tgttttcaag gtgcgcgcca tcataggcat ggggcaggtg gtggtcggct
     4441 gcgggattcc attcatgggt gtcgatgccg ttgaggatac cgtggatatc cttgctgcgc
     4501 gtgctcagca acccttgcat gccgaaaccg aattccggcg tctggatttc gcgggcgtag
     4561 gtcggactga ccgtggtgat gctgtcagcg tagtacaccc cggccttgag gaaggaaagc
     4621 tttccgtagt attccagtcc gtcgatatgg taagcctcca gaggcagccc cagcctggcc
     4681 acccagtctt ccgcacagtt gccctggaag gcgaggttat ggatacccag tactgacttg
     4741 gcgctgggtt tcccggcata gcgcatgaag gcaggcgtga gcccgccttg ccagtcgttg
     4801 aagtggacaa cgtccgggat ccagtcggcc agcacacttt gtttgctgcc aagcagcgct
     4861 gcgacatagg acaaaatgcc gaagcgcaag ggattgtcca gccactcgta ccccctggta
     4921 tcgagatagg ggccgctatc gcgttcgtaa agatgcggac aggcaatgac gatcaccggg
     4981 acgccggtat ccggcatttc gcctatcatc aggg
//
LOCUS       DAOMFA010000116         4933 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_2264_length_4933_cov_4.299713, whole genome shotgun sequence.
ACCESSION   DAOMFA010000116 DAOMFA010000000
VERSION     DAOMFA010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 4933)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4933)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4933
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2264_length_4933_cov_4.299713"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            241..474
                     /locus_tag="PKW44_06545"
     CDS             241..474
                     /locus_tag="PKW44_06545"
                     /inference="COORDINATES: protein motif:HMM:NF013677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HQR51278.1"
                     /translation="MSSRVERVEVLGPMQRRRRYSVDEKLRILEEAAVPGMTISFVAR
                     RHGISPSRLFHWRRRMAEGGKEAVRAVQSMAPG"
     gene            497..1975
                     /locus_tag="PKW44_06550"
     CDS             497..1975
                     /locus_tag="PKW44_06550"
                     /inference="COORDINATES: protein motif:HMM:NF014998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004144 - diacylglycerol O-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0045017 - glycerolipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HQR51279.1"
                     /translation="MIKMMPLDVAWFLLESKFVPVHGAFLYVFTPPVDARPDYAKRLL
                     ATMKKRAAGAPFNLLPRFRIAAMPCWEEVAVDLSRHVIETRLPAPGNDQQLLAAAAEL
                     AAPALDWKQPLWRIHWIDGLAGGRFAFMLVAHHSQWDGISIFRLMGETMSESASAKIV
                     RAPWQGVSTWIKLASGAAARQGKEGPHDDGQRQSALAQITDLLEDTASAATGMGRVFA
                     QQGWQALVRGRQVALPFAAAETRAERNGSSARTYGLAKIQAARVKAVAQATGASFNDV
                     MTSVADAAYVSYLAELGIVPGKRLVAMVPIALKIPGAGNQISNALVPLGAPGATPLAR
                     LAAVRTAMDNAKREIGGMGAASAKLFALVNTGIAAAPDLLGVGERLPVTANMMISNPY
                     GIPKALYLNGSRLDYFVPFVGPSLGTRLLVGIYTYAGETFVAITSLRSVVPTVERLSE
                     LVQVSFAELEQAAGVAQQPAAKRAAVAGQPAVRRRKAARTSV"
     gene            1977..2120
                     /locus_tag="PKW44_06555"
     CDS             1977..2120
                     /locus_tag="PKW44_06555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR51280.1"
                     /translation="MPAGFDVAATAMAGIAARGSDRENDPVTFFVATRGLQTVATLAA
                     SAA"
     gene            2117..2281
                     /locus_tag="PKW44_06560"
     CDS             2117..2281
                     /locus_tag="PKW44_06560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR51281.1"
                     /translation="MKRAFVLFLWSPAVVYYRQSNVPDLGAPAQRDGVKQSWSEQIWP
                     PSVSRRNERR"
     gene            2335..3894
                     /locus_tag="PKW44_06565"
     CDS             2335..3894
                     /locus_tag="PKW44_06565"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HQR51282.1"
                     /translation="MAIIDHFDRGWSINPDGIAYIQDERSFTFTEAGELSCQVAHALL
                     AGGYAAGTKGAVWSNNDVTAWICTLGLWRANLTWIPVGARNVIEDNQYILDMCDCEIM
                     FFQHYYSEAIAAIRPQLPKVKLWVCVDGEAEGAVSLDTWVKGQPKTNPKVPVNLDDVI
                     WLGSTGGTTGKPKGVMNTHRSVQTAMAHMIIVGNYEGDEKPVNLAAAPMTHTAGILSL
                     PVTMRGGTVVVITRPDPQLMLAAIPKHKVTELFLPPTVIYRLLEIPDLNKKVDLSSLK
                     YFFYGAAPMSVEKLKVALEVFGPVMTGSYGQTEAPGTISSLPPGDHFIGGKVAGDERL
                     SSVGKPNPLVRVEIMNDANEILPRGETGEICVRGDLVMKGYYKDSEKTAETIIDGWLH
                     TGDVGNIDAEGYLHITDRKKDMIISGGFNVYPGEVEQVIWGHPAVQDCAVIGMPDERW
                     GEAVKAVVELNKGQTVTAEEIIALCKKRLGSVKAPKMVDFIEQLPRSPVGKVLKRELR
                     AEYWQAANRKI"
     gene            complement(3995..>4933)
                     /locus_tag="PKW44_06570"
     CDS             complement(3995..>4933)
                     /locus_tag="PKW44_06570"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HQR51283.1"
                     /translation="IKKIIFNRYGSLDDLQMTDVQTPTFKSDELLIRVKVVSINPLDW
                     KRLEGQFKIITGFKFPKGIGIDFSGIVEKKGDAISGFQVGDAVFGGVDPMKDGALAEY
                     VVVKEAAIFKKPEAVSFETAAAAVTTGVTALHLSEKCHAQSGDSLLINGASGGVGMAV
                     LQMAKQRGIKVTAVASGEGLAFIQKWKPDATIDYKLQSVLEQKTAYNAIVELAGNIPF
                     GRAKAILKPQSIFASTLPSPVDMLKAFLNNLFSSKKFQIVIANTSPDYYRQLCELLTQ
                     KKLEIPIAKSFAMADFKEAYQFARKGGTVGKVVISI"
BASE COUNT         1033 a         1421 c         1474 g         1005 t
ORIGIN      
        1 tcctgacttt gagctcaagc gacctgacac ccaacttagc cccgttcgga gctagcagaa
       61 gtacgtggaa caagcacctg ccgcgcgctt gggcaatgac gaggatctca agggcctcat
      121 cctgttgcta gcgtcgcagg catcagacta catgagcggt caggccgggt ggagcagccc
      181 ccatttcaac cggacaccgc tgcatagcga gaggctaggt gtatgcctag gattacgact
      241 atgtcgagcc gtgtcgagcg agttgaggtt ttgggtccga tgcagcgtcg gcggcggtat
      301 tccgtcgacg agaagctgcg gatcctggaa gaggcagccg tgccgggcat gacgatttcc
      361 ttcgtcgccc gccggcatgg tatttctccc agccggctgt tccattggag acggcgcatg
      421 gccgaaggcg ggaaggaagc ggtccgcgca gtccaatcga tggcgcctgg ctgatccggg
      481 cgccacagga acgatgatga taaaaatgat gccgctggat gtggcctggt tcctgctcga
      541 atccaaattc gtgccggtgc atggagcctt tctgtacgtg tttacaccgc ctgtggacgc
      601 caggccggac tacgccaaac gtctgctggc gacgatgaaa aagcgtgcgg ctggcgctcc
      661 gttcaatttg ctgccgcgct tcaggatagc cgccatgccg tgctgggaag aggtggcggt
      721 cgatctctcc cggcatgtca tcgagacgcg cctgcctgca cccggaaacg atcaacagct
      781 gcttgcggcg gcggcggaac tcgccgcgcc ggcgctggac tggaagcagc cgctgtggcg
      841 cattcactgg atcgatggtc tggcgggcgg caggttcgcg ttcatgctcg tcgcgcatca
      901 ctcgcagtgg gacggcatca gcatattccg cctgatgggt gaaacgatgt ccgaatcggc
      961 cagcgccaag atcgtcaggg cgccctggca gggtgtttcc acctggatca agctggcatc
     1021 cggcgcggcg gcgcggcaag gcaaggaagg cccgcacgac gatggccagc ggcaatccgc
     1081 cttggcgcag atcactgatt tgctcgaaga cacggcaagc gcggcgaccg gcatgggccg
     1141 ggtgtttgcg cagcaaggct ggcaggcatt ggtacgtggc cgacaggtcg ccttgccgtt
     1201 tgccgctgcg gagacgcgcg ccgaacgcaa tgggtccagt gcgcgcactt atggcctggc
     1261 gaaaatccag gcggcgagag tcaaggcagt ggcgcaagcg accggggctt cattcaatga
     1321 cgtgatgacc tcggtggcgg atgccgccta tgtgagctat ctggccgaac tcggtatcgt
     1381 cccaggcaag cggctcgttg cgatggtgcc cattgcgctc aaaattcccg gcgcgggcaa
     1441 ccagatttcc aatgcgctgg tgccgctcgg cgcgcccggc gccacgccgc tggcgcgcct
     1501 tgcggcggtc aggactgcga tggacaatgc caagcgggaa attggcggga tgggcgcagc
     1561 gagcgccaag ttattcgccc tggtgaacac ggggatcgcc gccgcgccag acttgctggg
     1621 agttggcgag cgcctcccgg tgaccgcgaa catgatgata tctaatccct atggcatacc
     1681 gaaggcgctg tatctgaacg gcagccgcct agactacttt gtgccattcg tcggcccatc
     1741 gctgggcacg cggctgttgg tcggcatcta tacctacgcc ggggagacct tcgtcgcgat
     1801 cacctcgctg cgctccgtgg tgcccactgt cgaacgcttg agcgagttgg tacaggtgtc
     1861 gtttgccgaa ctcgagcagg ccgctggtgt ggcgcagcaa cccgctgcca agcgggcggc
     1921 cgttgcaggt cagcctgcgg tgcgccggag gaaggcggcg cggacgagcg tctgagatgc
     1981 cggccgggtt cgatgttgcg gcaacggcga tggccggtat cgccgcgcgc ggcagcgacc
     2041 gggagaacga tccggtcacg ttttttgtcg ccacgcgcgg gctgcaaacg gtggccacgc
     2101 tggcggcaag cgccgcttga aaagagcatt tgttctgttc ctgtggagcc cggcggtggt
     2161 ctattaccgg cagagcaatg tgcccgacct cggcgctccg gcacagcgtg acggcgtcaa
     2221 gcaatcgtgg tcggaacaga tctggccacc atccgtctcc cggcgaaatg aacggcgcta
     2281 atccgcaata ggcaatttga atttttgatc aactagaaat tcaaaggagg ggacatggca
     2341 atcatcgatc atttcgaccg cggctggagc atcaaccccg atggcatcgc ctacattcag
     2401 gacgagcgca gtttcacctt caccgaggca ggcgaactct cctgtcaagt ggcgcacgcg
     2461 ctgctcgccg gcggttacgc ggccggcacc aagggcgctg tctggtcgaa caacgacgtc
     2521 accgcctgga tctgcacgct gggtctgtgg cgcgccaacc tgacctggat tccggtcggc
     2581 gcgcgcaacg tcatcgagga caatcaatac atcctcgaca tgtgcgactg cgaaatcatg
     2641 tttttccagc actactactc cgaggccatc gccgccatcc gtccgcaact gcccaaggtc
     2701 aagctgtggg tctgcgtcga tggcgaagcc gaaggtgcgg tgtcgctgga cacctgggtc
     2761 aagggccagc ccaagaccaa cccgaaggtg ccggtcaatc tggatgacgt gatctggctc
     2821 ggctccaccg gcggcaccac cggcaagccc aagggcgtga tgaacacgca ccgcagcgtg
     2881 cagaccgcca tggcgcacat gattatcgtg ggtaattacg aaggcgatga aaaaccggtc
     2941 aatctggccg ccgcgccgat gacgcacacc gctggcattc tctcgctgcc cgtcaccatg
     3001 cgcggcggca ccgtcgtcgt gatcacccgg cccgatccgc agctgatgct ggcggcgatt
     3061 ccgaaacaca aggtgaccga actgttcctg ccgccgaccg tgatctatcg tctgctcgag
     3121 attcctgatc tgaacaagaa ggtcgatctg tcatcgctca agtatttctt ctacggcgcc
     3181 gcgcccatgt cggtggaaaa gctgaaagtc gcactagaag tcttcggtcc ggtgatgacc
     3241 ggcagctacg gccagaccga agcccccggc accatttcca gcctgccgcc cggcgaccat
     3301 ttcatcggcg gaaaggtggc cggcgacgag cgcctgtcgt cagtcggcaa gccgaacccg
     3361 ctggtgcgcg tcgagatcat gaacgacgcc aacgagattc tgccccgagg tgaaaccggc
     3421 gaaatctgcg tgcgcggcga cctggtgatg aagggctatt acaaagactc ggaaaagacc
     3481 gccgagacca ttatcgatgg ctggctgcac accggcgatg tcgggaatat cgacgccgaa
     3541 ggctatttgc acattaccga ccgcaagaag gacatgatca tcagcggcgg cttcaatgtt
     3601 taccccggcg aggtcgagca ggtgatctgg ggccacccgg cggtgcagga ttgcgccgtg
     3661 atcggtatgc ccgatgagcg gtggggcgaa gcggtgaaag ccgtggtcga actgaacaag
     3721 ggccagactg tcaccgccga ggaaatcatc gcgctgtgca agaaaaggct gggctcggtg
     3781 aaggcaccca agatggtgga cttcatcgaa caactgccgc gcagccccgt gggcaaggtg
     3841 ctgaagagag aactgcgtgc cgaatactgg caagccgcca atcgcaagat ctgaacttaa
     3901 aaaaccaacg tgaggaatgg gcccactcaa gcctatcctt cgacccgctt tttgggttgg
     3961 ggaggtatct gcgagccgag aatcgcccca gcagctaaat ggatatcacg actttgccca
     4021 ctgtgccgcc ttttcttgca aactgatagg cttctttaaa atcagccatt gcaaaactct
     4081 tggcaatcgg tatttccaat tttttttgtg tgagtaattc gcagagctgg cgataatagt
     4141 cgggcgaggt atttgctata acgatctgaa attttttgct cgaaaataga ttattgagaa
     4201 aggctttcag catatccaca ggactgggca gagtgctggc aaagatcgat tgaggtttca
     4261 aaatcgcttt tgctctgcca aacggaatat ttccagccaa ctcaacaatt gcgttgtagg
     4321 ctgttttttg ttccaaaaca ctttgtaatt tataatcaat cgtcgcatct ggtttccatt
     4381 tttgaatgaa ggcaagtcct tcgccactgg ctacagcggt cactttaatg cctcgttgct
     4441 ttgccatttg aaggactgcc ataccaacgc caccacttgc gccattgatg agcagactgt
     4501 caccgctctg agcatggcat ttctccgaaa ggtggagtgc cgtcacaccc gttgttacgg
     4561 ctgctgccgc cgtctcaaaa gacacagctt caggcttttt aaatatggcg gcctccttta
     4621 ccacgacata ctccgccaat gccccgtctt tcatgggatc tacgccacca aaaacagcgt
     4681 cacctacttg aaaacctgat atggcatcgc cttttttctc cacgattccc gaaaagtcaa
     4741 ttccgatacc ttttggaaat ttaaaacctg taatgatttt aaattgtcct tctagtcgct
     4801 tccaatctag agggttaata gaaacgacct taacccgaat caacagttca tcactcttga
     4861 atgtaggggt ctggacatca gtcatttgca gatcgtcaag actaccgtat cggttgaata
     4921 ttattttttt tat
//
LOCUS       DAOMFA010000117         4927 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_2267_length_4927_cov_7.741174, whole genome shotgun sequence.
ACCESSION   DAOMFA010000117 DAOMFA010000000
VERSION     DAOMFA010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 4927)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4927)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4927
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2267_length_4927_cov_7.741174"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            621..1508
                     /gene="prpB"
                     /locus_tag="PKW44_06575"
     CDS             621..1508
                     /gene="prpB"
                     /locus_tag="PKW44_06575"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046421 - methylisocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HQR51284.1"
                     /translation="MTALSPGQRFRNAVAEEHPLQIVGTINANHALLAKRAGFRAIYL
                     SGGGVAAGSLGVPDLGITGLDDVLTDVRRITDVCDVPLLVDVDTGFGSSAFNVARTVR
                     SMSKFGAAAVHIEDQVGAKRCGHRPNKEIVSLQEMVDRIKAAVDARIDNSFVIMARTD
                     ALAVEGLESALDRAAACIEAGADMIFPEAITELDMYSLFAQRVKAPILANITEFGSTP
                     LYTTEELASAGVSMVLYPLSAFRAMNKAAENVYTALRRDGTQKNVIETMQTRMELYDA
                     IGYHAFEQSLDALFAQKKG"
     gene            1623..2750
                     /gene="prpC"
                     /locus_tag="PKW44_06580"
                     /pseudo
     CDS             1623..2750
                     /gene="prpC"
                     /locus_tag="PKW44_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009851955.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050440 - 2-methylcitrate synthase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
     gene            2871..>4927
                     /gene="acnD"
                     /locus_tag="PKW44_06585"
     CDS             2871..>4927
                     /gene="acnD"
                     /locus_tag="PKW44_06585"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019921892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047456 - 2-methylisocitrate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe/S-dependent 2-methylisocitrate dehydratase
                     AcnD"
                     /protein_id="HQR51285.1"
                     /translation="MNTAFRKNLPGTALDYFDARAAVEAIKPGAYDNLPYTSRVLAEN
                     LVRRCAPATLDASLGQLIERKRDLDFPWFPARVVCHDILGQTALVDLAGLRDAIADKG
                     GDPAQVNPVVPVQLIVDHSLAVECGGFDPQAFDKNRAIEDRRNEDRFHFINWTKKAFK
                     NVDVIQPGNGIMHQINLEKMSPVIHSDRGVAYPDTCVGTDSHTPHVDALGVIAIGVGG
                     LEAENVMLGRASWMRLPEIVGVELTGKLAPNITATDLVLALTEFLRKQKVVGAYLEFH
                     GEGARALTLGDRATISNMAPEYGATAAMFAIDQQTIDYLKLTGREEQQVALVETYAKA
                     TGLWADSLANAEYERVLHFDLSSVVRNMAGPSNPHARVATSDLAAKGIAGAWEEVPGQ
                     MPDGAVIIAAITSCTNTSNPRNVIAAGLLARNANKLGLNRKPWVKSSLAPGSKAVQLY
                     LEEAGLEKELEQLGFGIVAFACTTCNGMSGALDPVIQQEIIDRDLYATAVLSGNRNFD
                     GRIHPYAKQAFLASPPLVVAYAIAGTIRFDIEKDVLGVVDGKEIRLKDIWPSDAEIDE
                     VVRAAVKPEQFRKVYIPMFAIEEDRGPKVTPLYEWRPMSTYIRRPPYWEGALAGERTL
                     RGMRPLAVLPDNITTDHLSPSNAILLDSAAGEYLAKMGLPEEDFNSYATHRGDHLTAQ
                     RAT"
BASE COUNT         1010 a         1597 c         1420 g          900 t
ORIGIN      
        1 tcatcattgc tctcgcgggt cattcatcca cttgtggtga ccttcccgta tttagcgctt
       61 tcattgctcc ggtttcagtg cattcccaaa caatgacccg acctctcatc acgtgggtac
      121 cagcgtcagc tgagacgtag acagcggaaa cgatccagta atggactggt atgccgggca
      181 acccgtttta gaccttgctc cctgatcaat cgatccctga tatcaccgtg ctgtgaccgc
      241 ccgggagtcc agcccagacc ttaattgtcg acattctgct tgagccaacc tagctaacgt
      301 gcccttacct acccgctgcg gcacgtatga cagtagatca gaaaatcaaa ataattattt
      361 ttgcgcgcct aatttgttga aaaatatgat gaaacgtcat ttttatctgc gtggctcttg
      421 tcttttcagg taggggggac ttgcacggca gaaatgggca agctaacaat gtgtcgacaa
      481 tttgattgtt tccaagcaag tacaagctgt tttttggctg tgttggcttg gtcagatgct
      541 gattgaaacg caccgcacgt gcaccttgga tcagctgtgt acgccatcca agcttgacct
      601 tatgagagag gacagcacat atgacggctc tttcccctgg ccaacgtttc cgaaacgcgg
      661 ttgctgaaga gcaccctttg caaattgtcg gcaccatcaa cgccaatcat gcgctgctag
      721 ccaaacgagc aggatttcgt gcgatctatc tctccggtgg tggcgttgca gcgggctcgc
      781 ttggggtccc ggatctcggc atcactgggc ttgatgatgt cttgacagac gtccgtcgca
      841 tcacggacgt ctgcgatgtt ccgcttttgg tagacgtgga cacgggcttt ggctcttcgg
      901 cattcaacgt cgcccgtacc gttcgatcca tgtcgaaatt tggcgccgct gctgtgcata
      961 tcgaggacca ggtaggtgct aagcgctgcg gtcaccgtcc gaataaagag atcgtctccc
     1021 tgcaggagat ggtcgaccgg attaaggctg ccgtggacgc ccggatagac aacagttttg
     1081 taattatggc tcgaaccgat gctttggcgg tcgagggtct agagtccgcc ctggatcgag
     1141 ccgctgcgtg catcgaagcg ggcgctgaca tgatctttcc agaggccatc acagagctgg
     1201 atatgtacag cctgttcgcg caacgcgtga aagcgccgat tctggccaac ataactgagt
     1261 tcgggtcgac gccgctctat accaccgagg aactcgcttc ggcaggcgtc tcgatggttc
     1321 tgtatccatt gtcagcattc cgcgccatga acaaggctgc cgaaaacgtc tacaccgcct
     1381 tacgccgcga cggcacgcaa aagaatgtaa tcgaaaccat gcagacccgc atggagcttt
     1441 acgatgccat tggctaccac gctttcgaac agagccttga tgcgctgttc gctcagaaga
     1501 agggctgaag aaatttagac cgagcggttg ggctcttagc gggtgcacca gccaagaagt
     1561 gaaacagaca aagccgatta gccagaaaga ttcctataag aagttcaaaa aaggagcaat
     1621 ccatggccca agcaaaagta ctcagtggcg ctggcctgcg tggccagatg gccggtcaga
     1681 ctgcactgtc gaccgtgggc caggccggtg ccggcctgac ctaccgcggc tatgacgtcc
     1741 gtgacctggc cgccggtgcc gaattcgaag aagtcgccta cctgctgctg tacggcgagt
     1801 tgccgagcaa gaccgaactg gccgactaca agcgcaagct caagggcctg cgtgacctgc
     1861 cacaggcact taaggaagtg ctcgagcgca tcccgcgcga cgcccacccg atggacgtaa
     1921 tgcgcaccgg ttgctcggtg ctgggcaccc tggagcccga gctgaccttc gaacaacagc
     1981 gcgacaagac cgatcgcctg ttggcgctgt tcccggcagt catgtgctaa tggtaccgct
     2041 tcacccatca tggcgtgcgc atcgattgca ccagcgacga agacaccctg ggcggccact
     2101 tcctgcacct gctgcacggc aagaagccga gcgaactgca cgtaaaggtg atgaacgtgt
     2161 cgctgatcct ctacgccgaa cacgaattca acgcctcgac cttcactgcc cgggtctgcg
     2221 cttcgaccct gtccgacctg tattcctgca tcaccgcggc catcggctcg ctgcgcggcc
     2281 cgctgcacgg cggcgccaac gaggcggcga tggaactgat cgagcgtttc cagagcccac
     2341 aggacgccac tgccgagctg ctgcgcatgc ttgagcgcaa ggacaagatc atgggcttcg
     2401 gccatgcgat ctacaaagag tccgacccgc gcaacgaggt gatcaagggc tggtcgaagc
     2461 agcttgccga ccaggtcggt gacacggtcc tgtacccggt ttccgaagcc atcgacaaga
     2521 ccatgtggga gcagaagcgc ctgttcccca acgccgactt ctaccatgcc tcggcgtacc
     2581 acttcatggg catccctacc aagctgttca cgccgatctt cgtctgctcg cgcctgaccg
     2641 gctgggccgc gcacgtgttc gaacagcgcg ccaacaaccg catcatccgc ccgagcgccg
     2701 agtatgtcgg cgtcgaacag cgccagttcg tgccgatcga acagcgctaa atcatagagt
     2761 gctggggctg ctgcgcagcc ctttcgcgac gcaaggccgc tcccacaggc gcagcgcccc
     2821 ctagggccag acctgcacac ccgaataccg tgaccgagcc tgataacgat atgaacacag
     2881 cattccgcaa gaacctgcca ggcacagccc tggactactt cgatgcccgc gccgccgtcg
     2941 aggccatcaa gcccggtgcc tacgacaacc tgccgtacac ctcccgcgta ctggccgaaa
     3001 acctcgtgcg ccgctgcgcg ccggccaccc tcgacgcctc gctgggccag ctgatcgagc
     3061 gcaagcgcga cctcgacttc ccctggttcc cggcccgcgt ggtgtgccat gacatcctcg
     3121 gccagaccgc cctggtcgac ctcgccggcc tgcgcgacgc catcgccgac aagggtggcg
     3181 acccggccca ggtcaacccg gtagtgccgg tgcagctgat cgtcgaccac tccctggccg
     3241 tggagtgtgg tggtttcgac ccgcaggcgt tcgacaagaa ccgcgccatc gaagaccgcc
     3301 gtaacgaaga ccgtttccat ttcatcaact ggaccaagaa ggcgttcaag aacgtcgacg
     3361 tgatccagcc cggcaacggc atcatgcacc agatcaacct ggagaaaatg tcgccagtca
     3421 tccacagcga ccgcggtgtg gcctacccgg acacctgcgt cggcaccgac agccacaccc
     3481 cgcatgtcga cgccctgggc gtgatcgcca ttggtgtggg cggcctcgaa gccgagaacg
     3541 tcatgcttgg ccgtgcctcg tggatgcgcc tgccggaaat cgtcggcgtc gagctgaccg
     3601 gcaagctggc gccgaacatc accgccaccg acctggtgct ggccctcacc gagttcctgc
     3661 gcaagcagaa agtggtcggc gcctacctcg agttccacgg cgaaggcgcc cgcgccctga
     3721 ccctgggtga ccgcgccacc atctccaaca tggccccgga gtacggcgcc accgccgcga
     3781 tgttcgccat cgaccagcag accatcgact acctcaagct gaccggccgt gaagagcagc
     3841 aggtcgccct ggtagaaacc tacgccaagg ccactggcct gtgggccgac agcctggcca
     3901 acgccgagta tgagcgggta ctgcacttcg acctgtcgag cgtggtgcgc aacatggccg
     3961 gtccgtccaa cccgcacgct cgcgtggcca ccagcgacct ggcggccaag gggattgccg
     4021 gggcctggga agaggtgccg gggcagatgc ccgacggcgc ggtgatcatc gccgccatca
     4081 ccagctgcac caacaccagc aacccgcgca acgtgatcgc cgccggcctg ctggcgcgca
     4141 atgccaacaa gctcgggctg aatcgcaagc cgtgggtcaa gtcgtcgctg gcgcccggct
     4201 ccaaggccgt acagctgtac ctggaagagg cggggctgga aaaggaactg gagcaactgg
     4261 gctttggcat cgttgccttc gcctgcacca cctgcaacgg catgtccggc gcgctggacc
     4321 cggtgatcca gcaggaaatc atcgaccgcg acctgtacgc taccgccgtg ttgtcgggca
     4381 accgcaactt cgacgggcgc atccacccct acgccaagca ggcgttcctg gcctcgccgc
     4441 cactggtggt ggcctacgcc atcgccggca ccatccgctt cgacatcgag aaggacgtgc
     4501 tgggcgtggt cgacggcaag gaaatccgcc tcaaggatat ctggccgagc gatgccgaaa
     4561 tcgacgaagt ggtgcgtgcg gcagtcaagc ctgaacagtt ccgcaaggtc tacatcccga
     4621 tgttcgccat cgaggaggac cgagggccga aggtgacgcc gttgtacgaa tggcgcccga
     4681 tgagcaccta catccgtcgt ccgccctact gggagggcgc cctggccggt gagcgcaccc
     4741 tgcgtggcat gcgcccgctg gcagtgctgc cggacaacat caccaccgac cacctgtcgc
     4801 cgtccaacgc catcctgctc gacagcgcgg cgggtgagta cctggcgaag atgggcctgc
     4861 cggaagaaga cttcaactcc tacgccaccc accgtggcga ccacctgacc gcccagcgcg
     4921 ccacctt
//
LOCUS       DAOMFA010000118         4876 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_2311_length_4876_cov_5.559428, whole genome shotgun sequence.
ACCESSION   DAOMFA010000118 DAOMFA010000000
VERSION     DAOMFA010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 4876)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4876)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4876
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2311_length_4876_cov_5.559428"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..594)
                     /locus_tag="PKW44_06590"
     CDS             complement(<1..594)
                     /locus_tag="PKW44_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQR51286.1"
                     /translation="MRVFITGASSGIGTALARHYAAQGARLGLAGRNRERLEALVASL
                     SADCTAYVLDVRDAEALRQAAADFIGKYGAPDIVIANAGVSRGTLTEHAEDASAFRAI
                     LDINLLGMVHTFQPFIAAMKAQGHGSLAGIASVAGLRGLPGAGAYSASKAAAISYLES
                     LRVELTGAGIRVTTICPGYIKTPMTDVNPYPMPFLMDA"
     gene            complement(601..1263)
                     /locus_tag="PKW44_06595"
     CDS             complement(601..1263)
                     /locus_tag="PKW44_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013149432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HQR51287.1"
                     /translation="MKVLLSALLLLVSSIAAADSPFDGRYEQTLQPIPTGNAAKIEVV
                     ELFWYGCPHCYDLEPALAAWVKKLPKDVEFKRVPGLPNPSWAPMAKAYYTMEALGIAD
                     KLHSPLFDAIHKQRTLKPNDEAGTIDWIIKQSGLDRKKVEDAYNSFSVNTKVTRAAQI
                     FRASGATGVPAIIVDGKYLTSIKLATKGNESISAAQANEQLFAVTDYLIDQLRKEKAA
                     KR"
     gene            complement(1275..1883)
                     /locus_tag="PKW44_06600"
     CDS             complement(1275..1883)
                     /locus_tag="PKW44_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HQR51288.1"
                     /translation="MTRDYKPAATRSKPAGKSSPFITGLLIGLILGIGVSIAVAIMLR
                     GGGNPFAVTTPPTVVTAPPDTGNATPPDALQQQQPAEKPRFDFYTILPGTETAVTEQQ
                     VQQSAAAQPGTPSEAGSKNYFLQVGAFQNEQDADNLKAQLALLGLEAVVQTANLPDKG
                     VSHRVRVGPLTDIEQIRKARTELTKNGFSPFLIQQNSMTPDQ"
     gene            complement(1900..3555)
                     /gene="argS"
                     /locus_tag="PKW44_06605"
     CDS             complement(1900..3555)
                     /gene="argS"
                     /locus_tag="PKW44_06605"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019880745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HQR51289.1"
                     /translation="MKAHLTELLAQAAKAVTPELADLSVVLERPKSAEHGDFATNLAM
                     TLARTLKQNPRAIAQSLITALPASPYVARTEIAGAGFINFFLNPAAKAGVVREILGHA
                     AWGRNNTGQGRKLQVEFVSANPTGPLHVGHGRGAAVGDCLCRLLEATGWQVTREFYYN
                     DAGAQIDNLTRSVQCRAKGLTPDDPAWPEDGYRGDYITDVAKAYMAKEKIDAEDKHVI
                     GAGDADDAAAIRAFAVAYLRREQDLDLRAFDVHFDVYSLESALYTEGKVEATVKSLIA
                     SGHTYEQDDALWLRTTSFGDDKDRVMRKREGGYTYFVPDVAYHLDKWQRGFSRVINEQ
                     GADHHSTITRVRAGLQALGIGIPQGWPDYVLHQMVTVMRGGEEMKISKRAGSYVTLRD
                     LIDMVGRDATRYFLAARRADSQLVFDIDLALAHTNDNPVYYIQYAHARICSVLEQWGG
                     DVSSLAEADLSPLQLPHELGLMQRLAEFPETLENAANELAPHLIAYYLKDLAGDLHSY
                     YNAEKFLVEDEAVKHARLALVGATRQVLQGGLQLLGVSAPNEM"
     gene            3628..>4876
                     /locus_tag="PKW44_06610"
     CDS             3628..>4876
                     /locus_tag="PKW44_06610"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HQR51290.1"
                     /translation="MTILRVALDVPLARLFDYLDTGSGVQVGQRVLVPFGRRRAVGIV
                     LERAASSDMATDKLKPIVRAFVEEPPLPLATLKLLKFCSEYYHYPLGQTILNALPSRL
                     REGEPACLPRRTGYRLTAAGIEAGERAIPGRAVLQKKLYLALQASGTLDAAAMAAIAG
                     GWRPALRQLLDQGWVEAVEIEAVLPDQIAMTATAPKLNTDQQAALEAIQAAGPDFGVW
                     LLHGITGSGKTEVYMHLLQAMLAGPGRQALVLVPEINLTPQLEARFRARFPHHPIVML
                     HSQMNERERLHGWLRAQSGEARIVIGTRLSVFTPMQQLAVIVVDEEHDASYKQQDGMR
                     YSARDVAIMRGKMVNVPVLLGSATPSLESWHNAQAGRYRLLTLRERAVSAAALPQIHC
                     IDTRPNPPQDGISTALIGALKLRL"
BASE COUNT          848 a         1460 c         1580 g          988 t
ORIGIN      
        1 ggcatccatc aggaagggca tggggtaggg gttgacgtca gtcatggggg tcttgatgta
       61 gccggggcag atcgtggtga cgcggatgcc ggcgccggtc agttccaccc gcaggctttc
      121 cagatagctg atggcagcag ccttggaggc ggagtaggcg cccgcgccgg gcaatccacg
      181 caggccggcg acgctggcga tgccggcgag cgaaccatgg ccttgcgctt tcatggcagc
      241 gatgaagggt tggaaagtgt ggaccatgcc cagcaggttg atgtcaagaa tggcacggaa
      301 ggcgctggcg tcttcggcat gttcggtgag ggtgccgcgc gacacgccag cgttggcgat
      361 gacgatgtcg ggggcgccgt atttgccgat gaaatccgcc gccgcctgcc gcagcgcctc
      421 ggcatcgcgc acatccagca cataggcagt gcagtcggcg gagaggctgg ccaccagtgc
      481 ttccagccgt tcccggttgc gtcccgccag accgagccgc gcgccctgcg ccgcgtaatg
      541 acgcgccagc gcggtgccga tgccgctgga agcgccggtg atgaagacgc gcatcccggt
      601 ttagcgtttg gccgcttttt ccttgcggag ctgatcgatc aggtaatccg tgacggcaaa
      661 cagttgctcg ttggcctggg cggcgctgat gctttcattg cccttggtgg cgagcttgat
      721 ggaggtcagg tatttgccat cgacaatgat ggccggcacg ccggtagcgc cagaggcacg
      781 gaatatctgc gccgcgcgcg tgaccttggt gttgacggag aaggagttgt aggcgtcttc
      841 cactttcttg cgatccagcc cgctctgctt gatgatccag tcgatagtgc cagcctcgtc
      901 gttgggcttg agcgtccttt gcttgtggat ggcatcgaac agcggactat gcagcttgtc
      961 cgcgatgccc agtgcttcca tggtgtagta ggccttggcc atgggtgccc aactcgggtt
     1021 gggcaggccg gggacgcgtt tgaattcgac atccttgggt agcttcttga cccaggcggc
     1081 cagcgccggc tcgaggtcgt agcaatgcgg gcaaccatac cagaacagct cgacgacctc
     1141 gatcttggcg gcattgccgg tggggatggg ttgcagggtc tgctcatagc ggccgtcaaa
     1201 tggggaatcg gcggcggcga tgctggaaac cagcagcagg agtgcggata aaaggacttt
     1261 catggggttt tcctctattg gtctggagtc atgctgtttt gctggatcag gaacgggctg
     1321 aagccatttt tggtcagttc ggtacgcgct tttctgattt gctcgatatc ggtcaaagga
     1381 ccgacgcgta cgcgatgcga tacgcccttg tccggcagat tggcggtttg caccacggct
     1441 tccaatccca gcagcgccag ttgtgccttc aggttatcgg catcctgctc gttctggaat
     1501 gcgcccacct gcaggaagta gtttttgctg cctgcttctg atggagtgcc tggttgcgcg
     1561 gcagcggatt gctgcacttg ctgctccgtg acggcggttt cggtgcccgg cagaatggtg
     1621 tagaaatcga aacgcggttt ttcggcaggc tgctgttgct gcaatgcgtc tggtggagtc
     1681 gcattccctg tgtccggcgg ggccgtcact accgtagggg gtgtcgttac ggcgaaaggg
     1741 ttgccaccac ctcggagcat gatggcaaca gcgatggaaa cgccgatgcc caggatcagc
     1801 ccgatcagca ggccagtgat gaaagggctg cttttgcctg ccggttttga cctggttgcg
     1861 gctggtttat agtcacgtgt cacgtgtgtt ttccttgagt tacatttcgt tcggggcact
     1921 tacgcccagc aattgcaaac cgccttgcag tacctggcga gtggcgccca ccaaggccag
     1981 gcgggcatgc ttgacggcct cgtcctcgac caggaatttt tcggcattgt agtagctgtg
     2041 caggtcgcct gccagatcct tgaggtagta ggcaatcagg tgcggcgcaa gttcattggc
     2101 ggcgttctcc agcgtttccg ggaattccgc caggcgctgc atcagcccca gttcgtgcgg
     2161 taattgcagc ggcgacaggt cggcctctgc aagactggat acatcgccgc cccattgctc
     2221 cagtacgctg caaatacggg cgtgggcgta ctggatgtag tacaccgggt tgtcgttggt
     2281 gtgggccagc gccagatcga tgtcgaacac cagttgcgaa tcggcgcggc gcgcagcgag
     2341 aaagtagcgc gtggcgtcgc ggccgaccat gtcgatcagg tcgcgcaggg tgacgtagct
     2401 gccggcacgc ttggaaatct tcatctcctc accgccgcgc atcaccgtca ccatctgatg
     2461 cagcacataa tccggccagc cctgcggaat gccgatgccc agtgcctgca ggccggcgcg
     2521 cacgcgggta atggtgctgt ggtggtcggc gccctgttcg ttgatgacgc ggctaaagcc
     2581 acgctgccac ttgtccaagt ggtaggccac gtccggcacg aagtaggtgt agccgccttc
     2641 gcgcttgcgc atgacgcggt ccttgtcgtc accgaaactg gtggtgcgca gccacagcgc
     2701 atcgtcctgc tcgtaggtat ggccgctggc gatcaggctc ttcacagttg cctcgacctt
     2761 gccctcggta tacagcgccg actccagcga atagacatcg aaatgcacat cgaaggcccg
     2821 cagatccagg tcctgctcgc ggcgcaggta ggccacggcg aatgcacgga tggccgcggc
     2881 atcgtccgcg tcgccggcac cgatgacatg cttgtcctcg gcgtctattt tttccttcgc
     2941 catgtaggcc ttggcgacat cggtgatata gtcgccgcgg tagccgtctt ccggccaagc
     3001 cgggtcgtcc ggtgtcagcc ccttggcgcg gcactgcacg gagcgggtca aattgtcgat
     3061 ctgcgcgcca gcgtcgttgt aatagaactc gcgtgtcacc tgccagccgg tggcctccag
     3121 caaccggcac aggcagtcgc ccactgccgc gccacgcccg tggccgacgt gcagcgggcc
     3181 ggtggggttg gcggagacga attccacctg cagcttgcgt ccttgcccgg tattgttgcg
     3241 tccccaggcg gcgtggccga gaatctcgcg caccacgcca gccttggctg ccggattcag
     3301 gaagaaattg atgaagccgg cgccggcgat ctcggttctg gcgacgtagg gcgaagccgg
     3361 caaggcagtg atcaggctct gcgcaatggc gcgcgggttc tgcttcagcg tgcgcgccag
     3421 cgtcatcgcc aggttggtgg caaaatcgcc gtgctcggcg gattttggac gttccaatac
     3481 gacggacagg tcagccagtt ccggcgtcac ggcttttgct gcctgtgcca gtaattcggt
     3541 aagatgtgct ttcaaggcaa ttctaacgac ggggaaaagg cgttatttta gcggtttccc
     3601 gacaataacg ctttcctaat aaatacgatg acaatccttc gcgtcgccct cgatgtcccg
     3661 ctcgctaggc tgttcgacta cctggacacc ggcagtggcg tgcaggttgg tcagcgtgta
     3721 ttggtgccgt tcgggcgacg gcgagcggtt ggcatcgtgc tggaacgtgc cgcctcgtcc
     3781 gacatggcca cggataagct caagccgata gtacgcgcct ttgtggagga gccgccgcta
     3841 ccgctggcaa cactcaagct gctcaaattc tgctccgaat attaccacta cccactcgga
     3901 caaacgatcc tcaatgcctt gccgtcccgc ttgcgcgagg gcgagcctgc ttgcctgccg
     3961 cgccgcactg gctatcggct gacagcagcc ggcatcgaag ccggagagcg cgctatcccg
     4021 ggccgggccg tcctgcagaa aaaactctat ctggcgctgc aagcatcagg caccctggat
     4081 gccgcggcaa tggcagccat cgccggcggc tggcgccccg cgctgcgaca attattggat
     4141 cagggctggg tcgaggcggt tgaaatcgaa gccgtgctgc cagaccagat agcaatgaca
     4201 gccacggctc ctaagctgaa caccgaccag caggcggcgc tggaggccat ccaggccgcc
     4261 ggtcccgatt tcggcgtctg gctgctgcat ggcatcaccg gcagcggcaa gaccgaggtt
     4321 tacatgcacc tgttgcaggc gatgctggct ggccccggcc gacaggcgct ggtgctggtg
     4381 ccggagatca acctcacgcc gcaactggaa gcacgtttcc gcgcccgctt cccgcaccac
     4441 cccattgtca tgctgcacag tcagatgaac gagcgcgagc gcctgcatgg ctggctgcgt
     4501 gcgcagagcg gcgaggcgcg tatcgttatc ggtacgcggc tgtccgtatt tacacccatg
     4561 cagcaactgg cggtgattgt cgtggacgag gagcacgacg cctcctacaa gcagcaggac
     4621 ggcatgcgct actcggcgcg cgatgtggcg atcatgcgcg gcaagatggt caatgtgccg
     4681 gtgttgctcg gctccgccac gcctagcctg gaaagctggc acaacgccca agccggccgc
     4741 taccggctgc tcacgctcag ggaacgcgcc gtcagcgccg ccgccctgcc gcaaatccat
     4801 tgcattgata cccgccctaa cccgccgcag gacggcatct cgaccgcctt gatcggcgcg
     4861 ctcaaactaa ggctgg
//
LOCUS       DAOMFA010000119         4845 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_2327_length_4845_cov_4.581420, whole genome shotgun sequence.
ACCESSION   DAOMFA010000119 DAOMFA010000000
VERSION     DAOMFA010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 4845)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4845)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4845
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2327_length_4845_cov_4.581420"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..582)
                     /locus_tag="PKW44_06615"
     CDS             complement(<1..582)
                     /locus_tag="PKW44_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008064088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HQR51291.1"
                     /translation="MQLERILHSQGFGSRKLCRILCRDSRVMVNGETCDDPFADIPTK
                     YLHFSVDGEPWEYREKAYLMLNKPAGYECSQKPSHHPGVYALLPSPLRERGVQSIGRL
                     DEDTTGLLLFSDDGQFIHKMSSPKWKVPKVYEVTTRHPVDTGLTAALVTGVQLIDEPA
                     PIAALACEAVAEHVLHLTLAEGKYHQVKRMVAAA"
     gene            complement(572..1096)
                     /locus_tag="PKW44_06620"
     CDS             complement(572..1096)
                     /locus_tag="PKW44_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HQR51292.1"
                     /translation="MRSKNPTTIRPLYADITTLAVDAIVNAAAPALLGGGGVDGAIHA
                     TAGPGLLEECRLLGGCTTGGAKITRGHQLPAKHVIHTVGPVWRGGSRNEVELLRACYL
                     NSLDLAVAAQARSIAFPCISTGVYGYPVVEASTIAVATVKEFCATNDTLHEIIFCCFS
                     TETLQIYRELLHAA"
     gene            1124..1924
                     /locus_tag="PKW44_06625"
     CDS             1124..1924
                     /locus_tag="PKW44_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019897995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HQR51293.1"
                     /translation="MTTTLILAMKSIISRDNPTYKQLKKLAESARERRKTGHALLDGA
                     HLVAACLAAGRLPEMLVVAESAAGNAEISGLLANTAGAQKIRLSDALFAEISPVETPT
                     GLLAVVEIPHFPMLTRPEFGLLLEDVQDPGNLGSILRSAAAAGVQAAWLSAGCADAWS
                     PRVLRAGMGAHFVLPVVERADLTAVATGFPGQVLAACLEGESLYRLDLAGPVAFMIGN
                     EGAGLSRELIAAAGQRFTIPMPGRVESLNAAAAAAVCLFERVRQTHGL"
     gene            2008..2283
                     /locus_tag="PKW44_06630"
     CDS             2008..2283
                     /locus_tag="PKW44_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HQR51294.1"
                     /translation="MSHKHINLIRAIFHDPVSGNIHWREIESLLHHLGASITPAHGAR
                     FHVKLNGFDEFLHHPHQSNVYGKQEVKHLREFLARAGVTPSLYEDGE"
     gene            complement(2361..3239)
                     /gene="hslO"
                     /locus_tag="PKW44_06635"
     CDS             complement(2361..3239)
                     /gene="hslO"
                     /locus_tag="PKW44_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HQR51295.1"
                     /translation="MKPDLDTLTRFLFEHAAIRGELVHLDATWQTVLEHHDYPAVLRQ
                     AMGELMAAAALLAATLKLKGSLVLQIQGKGPITLLVVECTGELTMRATAKWTGALPDG
                     GLAQLIGEGRFVITLDTKDGKQPYQGIVPLEGGSVAEILQNYMTRSEQLETRLILAAT
                     DQQAAGLLLQKLPQKAGQDEEAWTRIGHLADTLKSSELLDLPAQDILRRLFHEEDLRL
                     FEPQAVSFSCNCSRENVANMMRMLGRTEVDSVIAERGSLEAHCEFCNQRYVFDAVDVE
                     QVFSTEIAAPGSQTRH"
     gene            complement(3236..4129)
                     /gene="gluQRS"
                     /locus_tag="PKW44_06640"
     CDS             complement(3236..4129)
                     /gene="gluQRS"
                     /locus_tag="PKW44_06640"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HQR51296.1"
                     /translation="MADLYRGRFAPSPTGPLHFGSLVAAVGSFLEARRHGGKWLVRME
                     DLDPPREAPGAANLILHTLETYGFEWDGEVLYQSRRADAYEAALRQLEQASMLYPCAC
                     TRKEIADSTMRGIEGPVYPGTCRSGLHGRPARAWRVRTTAEEITFDDGIQGRQTQVLE
                     RDIGDFVLKRADGFHAYQLAVAVDDAAQGITHVVRGADLLDSTPRQVWLQRLLGLPAL
                     HYAHLPVAVNAVGEKLSKQTLATPLDPLHPAPVLWQALDFLGQQPPPELCDELSLLWQ
                     WAQANWRLEAVPKTRDKMPLL"
     gene            complement(4129..4455)
                     /locus_tag="PKW44_06645"
     CDS             complement(4129..4455)
                     /locus_tag="PKW44_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3301 domain-containing protein"
                     /protein_id="HQR51297.1"
                     /translation="MEITLLLVMLAAAWFWLDSLHKREIAMAAGRQAAERYGLQFLDE
                     TVAISRLRVGRDHNGRLQLRRTYGFEVSDTGADRLPCSVTLLGQRIEALDMPPYRDNV
                     IPFPLH"
     gene            complement(4462..>4845)
                     /locus_tag="PKW44_06650"
     CDS             complement(4462..>4845)
                     /locus_tag="PKW44_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pili twitching motility protein PilT"
                     /protein_id="HQR51298.1"
                     /translation="LVPKKGGGRCAAIEILLNSPLVADLILKGETNMIKEAMAKSVEL
                     GMQTFDQALFSLCEEGRITEEDALRNADSINELRLKFKLHGKNSNVKDADSRIDHLSL
                     HEDKEEEAPEEVPPGVPAAEAALVQ"
BASE COUNT          863 a         1432 c         1570 g          980 t
ORIGIN      
        1 ggcggcggcc accattcgct tcacctggtg atacttgcct tcggcgagcg tgagatgcag
       61 cacgtgttcg gcaacggctt cgcaggccag cgcggcgatg ggtgccggtt cgtcgataag
      121 ctgcacgccg gtaacgagcg cggcggtcag gcctgtatcg accggatgcc tggtggtgac
      181 ttcatagacc ttgggcactt tccacttcgg cgaactcatt ttatggatga actggccgtc
      241 gtcggaaaac agcagcagtc cggtagtgtc ttcgtccagt cgaccaatcg actgcacgcc
      301 gcgttccctc agcggcgagg gcagcagggc gtagacgccg gggtggtggc tgggcttttg
      361 cgagcattcg tagccggccg gcttgttgag catcaggtag gctttctcgc ggtactccca
      421 gggttcgccg tccacgctga aatgcaggta cttggtgggg atatctgcaa acgggtcatc
      481 gcacgtttcg ccgttcacca tgacacgact gtcgcggcag aggatgcggc acaacttgcg
      541 cgaaccaaag ccctgcgaat ggaggatgcg ttcaagctgc atgcaatagt tcccggtaaa
      601 tttgcaatgt ctcggtcgag aagcagcaga aaatgatctc gtgcagcgta tcgttggtgg
      661 cgcagaattc cttgaccgtg gcgactgcta tcgtggatgc ctccacgaca ggatagccgt
      721 aaacgccggt gctgatgcag gggaaggcaa tggagcgtgc ttgtgctgct acggccagat
      781 ccagcgaatt gagatagcag gcgcgcagca attccacttc gttgcggcta ccgccccgcc
      841 acaccggacc gacggtatgg atgacgtgtt tggccggaag ctggtggccg cgcgtgattt
      901 ttgctccgcc ggtggtacat ccgcccagca gccggcactc ctccagcagg ccgggcccgg
      961 cggtggcatg gatggcgccg tccacgccgc cgccgcccag caatgcaggt gcagccgcgt
     1021 tgacgatggc atcgactgcc agtgtagtga tgtcagcgta caaggggcgg atggtggttg
     1081 ggtttttcga tctcatgccg gctttctccg ccgtataatg gccatgacaa cgaccttgat
     1141 actggcgatg aaaagcatca tatcccggga caaccccacc tacaagcaac tgaagaagct
     1201 ggccgaatcg gcgcgcgagc ggcggaagac tgggcatgcg ctgctggatg gcgcacacct
     1261 ggtcgcagcc tgcctggctg ccggcaggct gccggaaatg ctggtggtgg cagaaagcgc
     1321 ggcgggcaat gccgagattt ccggcttgct tgcaaacact gccggtgcgc agaaaatccg
     1381 gttgtccgat gccttgttcg cggaaatctc gccggtagaa acgcctaccg gcctcctggc
     1441 cgtggttgaa ataccgcatt ttcccatgct gaccaggcct gaattcggcc tgctgttgga
     1501 ggacgtacag gaccctggca acctgggatc catcctgcgc tcggccgccg ccgccggggt
     1561 gcaggctgcc tggctttccg ccggctgcgc tgatgcctgg tcgccacgcg tgctgcgcgc
     1621 tggcatgggg gcgcatttcg tgctgcccgt cgttgagcgt gctgacctga cggctgtcgc
     1681 caccggtttt cccgggcagg tgctggccgc ctgtctggaa ggcgaatcac tttaccggct
     1741 cgacctcgcc gggccggtgg cgttcatgat cggcaacgag ggcgcgggat tgagccggga
     1801 gttgatcgca gcggccggcc agcgcttcac catccccatg ccggggcggg tggagtcgct
     1861 caacgctgct gctgctgctg cggtttgttt gtttgagcgg gtgcgacaga cgcacggact
     1921 atgatggcgt ggactgtgag tcccttgttg ctggaaaaag tctttgcact ggcggcataa
     1981 tgttgatact gaagggagag cctcaccatg agccacaagc atatcaacct gatccgtgcc
     2041 atcttccatg atccggtcag tggcaatatt cactggcgcg agattgaatc cttgctgcac
     2101 catctcggcg ccagcatcac acccgcgcat ggtgcgcgtt tccacgtcaa gctgaatggt
     2161 ttcgatgagt tcctgcatca cccgcatcag agcaacgtct acggcaagca ggaggtcaag
     2221 cacctgcgcg aattcctcgc ccgtgccggc gtcacgccat cgctttatga ggacggtgag
     2281 taaagctgtc cctgggcaag taattgatgg gaatcaacca tcagccggca tcgcgtcgat
     2341 gccatgcaat tgaacgggtt tcaatgccgc gtctggctgc cgggtgcggc gatctccgta
     2401 gaaaacacct gttcgacatc gaccgcgtca aacacgtagc gctggttgca gaattcgcag
     2461 tgcgcttcca ggctgccgcg ttcggcgatg acgctatcga cttccgtacg gcccagcatg
     2521 cgcatcatgt tggcgacgtt ttcccgtgag cagttacagg agaagctgac cgcctggggt
     2581 tcgaacaggc gcagatcttc ctcgtggaac aggcggcgca ggatgtcctg tgccggcaag
     2641 tccagcaatt ccgaggattt cagcgtgtct gcgaggtgac cgatacgtgt ccaggcctct
     2701 tcgtcctgcc cggctttctg aggcagtttt tgcagcagca ggccggcggc ctgctggtcg
     2761 gtagcggcga gaatgaggcg ggtttccagt tgctcggagc gggtcatgta gttttgcagg
     2821 atttcagcca cgctgccgcc ttccagcggc acgatgccct gataaggctg cttgccatcc
     2881 ttggtgtcca gcgtgatgac gaagcggcct tcgccgatca gttgcgccaa gccgccatct
     2941 ggcagggcgc cggtccactt ggcggtagcg cgcatggtca gttccccggt gcattccacc
     3001 accagcagtg ttatcggccc cttgccctgg atttgcagca ccagcgagcc tttgagttta
     3061 agggttgcgg ccagcagcgc ggcggcggcc atcagctcac ccatggcctg gcgcagcacc
     3121 gccgggtagt cgtgatgctc cagcacggtc tgccaggtgg cgtccaggtg caccagctcg
     3181 ccgcggatgg cagcgtgttc gaacaggaag cgggtgaggg tgtcgaggtc aggtttcata
     3241 ggagcggcat cttatcacgt gttttcggga ccgcttccaa ccgccagttg gcctgggccc
     3301 attgccacag cagagagagc tcatcacata attccggcgg cggttgctga ccgagaaaat
     3361 ccagcgcctg ccacagcacc ggggcaggat ggagcgggtc tagcggggtg gccagggtct
     3421 gcttggacag tttttcgccg acggcattga ctgccaccgg caggtgggcg tagtgcagag
     3481 cgggcagacc gagcaggcgc tgcagccaga cttggcgcgg agtcgaatcc agcaggtcgg
     3541 cgccacgcac cacgtgggtg atgccctgcg ccgcgtcgtc cactgcgacc gccaattgat
     3601 aggcatgaaa gccgtcggcg cgcttgagta cgaagtcgcc gatatcgcgc tccagcacct
     3661 gtgtctgacg gccctggatg ccgtcgtcga aggtgatttc ctccgccgtg gtgcgcaccc
     3721 gccaagcccg cgccggccgg ccgtgcaaac cgctacggca ggtgccaggg taaaccgggc
     3781 cttcgatccc ccgcatggtg gaatcggcaa tttccttgcg ggtacaggcg caagggtaga
     3841 gcatactggc ttgttccaac tgtcgcagtg cggcctcgta agcatcggcg cggcggctct
     3901 ggtacaacac ttcgccgtcc cattcaaagc cgtaggtctc cagggtgtgc aggatcaggt
     3961 tggcagcccc tggtgcttcg cgtggcgggt cgagatcttc catgcgtacc agccattttc
     4021 cgccatggcg cctggcttcc aggaagctgc cgacggcggc gaccagcgag ccgaagtgta
     4081 atggcccagt gggcgagggg gcgaaacggc cgcggtaaag gtcggccatc agtgcaaggg
     4141 gaagggaatc acgttgtcgc gataaggcgg catgtcgagc gcctcgatcc gctggccgag
     4201 cagggtgacg ctgcatggaa gacggtcggc gccagtgtca ctgacctcga agccataggt
     4261 acgccgcagt tgcagccggc cgttatggtc gcgcccgacg cgcagtctgg agatagcgac
     4321 agtttcatcg aggaactgca gcccatagcg ttccgcggcc tgtcggccgg cggccatggc
     4381 gatttcacgt ttatgcaggc tgtccagcca gaaccaggcc gccgccagca tgaccagcaa
     4441 cagggtgatt tccatcgtaa cctactgcac cagtgcggct tctgcggcag ggacacctgg
     4501 aggcacttct tccggcgctt cctcttcctt gtcttcgtgc agcgaaaggt gatcgatgcg
     4561 cgaatcggcg tccttgacgt tggaattctt gccgtgcagc ttgaacttga ggcggagttc
     4621 gttgatcgag tcggcattgc gcagcgcgtc ctcctcagtg atgcggcctt cttcgcacag
     4681 gctgaacagc gcctggtcga aggtctgcat gccgagttcg accgacttgg ccatggcttc
     4741 cttgatcatg ttggtttcgc ccttgaggat caaatcggct accagcggcg agttgagcag
     4801 tatttcgatg gcggcgcaac ggccaccacc ttttttggga accag
//
LOCUS       DAOMFA010000120         4808 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_2357_length_4808_cov_5.530823, whole genome shotgun sequence.
ACCESSION   DAOMFA010000120 DAOMFA010000000
VERSION     DAOMFA010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 4808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4808
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2357_length_4808_cov_5.530823"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..361
                     /locus_tag="PKW44_06655"
     CDS             <1..361
                     /locus_tag="PKW44_06655"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HQR51299.1"
                     /translation="LKTVHYEATRAGLDPQLVLGLIQVESGFNKYAVSTVGARGFMQV
                     MPFWVKSIGAPGQNLFHLRANLRYGCTILRHYLDIENGDLYRALGRYNGSVGKPEYPN
                     LVVGAWKRHWAYSPVRS"
     gene            543..1046
                     /locus_tag="PKW44_06660"
     CDS             543..1046
                     /locus_tag="PKW44_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020183761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HQR51300.1"
                     /translation="MNQLTALSLSIAVLGGLWVFLALGPLSGLVLVWAGFIAWGCYFH
                     TGGDAAALKKTIAGMIYGAILAGVALFLLGTNPLHLPAVAAAPVYVAVTVFFLVIVAG
                     CNLLSVVPANVYGYAATVAYAVQTANAPLVTDLIMANPVIVLSLSFVLGAVLGMLSGK
                     LAGVLGK"
     gene            complement(1142..1624)
                     /locus_tag="PKW44_06665"
     CDS             complement(1142..1624)
                     /locus_tag="PKW44_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2242 domain-containing protein"
                     /protein_id="HQR51301.1"
                     /translation="MILIPLLLASCGGPRGYAETEGFQSDSRYRRDFQVAVAPLCDAA
                     RRALLSDGYVVTKREGQSLSGGKEFQGEEKIHAILQVYVSCEQRAGGSTLFVTATEEH
                     FDVKASSESTLIGIPLLAPISFGTSSEADNQVKIRGETVTKRDFYERFYRAVQRELPH
                     "
     gene            complement(1917..2804)
                     /locus_tag="PKW44_06670"
     CDS             complement(1917..2804)
                     /locus_tag="PKW44_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019899614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR51302.1"
                     /translation="MNDKRWRLAIAGALAMILSLPAGAQVLAEALEGMRIPLAARPVS
                     RPMTVAYVPEQARYIIADGGFAAMADEYGNIASKSLIHVYDAKGNYVSSAKPGLNNKT
                     VYFNPNQNRLETVTYNVSSDVGMLPLCGVYSLELDKEGKLTNNSDMLSGPNPAFGDAA
                     TFPTYDPAENQYYAKQGRSDRVLVVKLDKREPVREIKLDLAAAGARFDDISDSYVAFT
                     GIPGEELAVLDVDHKAVLVFDLKGKFVGRSALPSAMKLRANNHYSGHGYANGMFFVYH
                     ESEGEFGTYYGFRISDQAK"
     gene            2852..3928
                     /locus_tag="PKW44_06675"
     CDS             2852..3928
                     /locus_tag="PKW44_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR51303.1"
                     /translation="MDKHPHLLVFISSHGFGHIAQTAPVLNALRKLIPGLALTIRSTA
                     PLGQLRSRIHGEFEYLPLAGDIGMLMSSALDVRAAESAAAYRELHGAWEARVASEATL
                     LRDIAPDFVLSNIGYLPLAGAQRAGIPCAAMCSLNWADIHSHYCQDPAITAQIHRAYA
                     GAQAFLRLTPGMAMVDLPNRILIDAVAEVGRNRREEINHRLGLGYEDKLVLVSLGGIS
                     GRLPIETWPRQPGVRWLVQTDWRAEHPNAVALESLQMEFSDLLASSDALVCKPGYGSF
                     VEAACSGVPALFASRADWPESSALTDWLKENGLCREVSRQALEGGDFATELQAMWDAT
                     RPEPVHPSGIAQAADWLAARLLPR"
     gene            complement(3921..>4808)
                     /gene="queA"
                     /locus_tag="PKW44_06680"
     CDS             complement(3921..>4808)
                     /gene="queA"
                     /locus_tag="PKW44_06680"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HQR51304.1"
                     /translation="QPSDLLVFNDTRVLKARLNGRKESGGKIEVLVERVLDDKRCLAH
                     IRASHAPRPGARLWLAGDAIEVEVLARQDDLYELGFAGDKALLDLLERHGSLPLPPYI
                     THAADDTDEERYQTVYARQPGAVAAPTAGLHFDAALLAQLTGIGVSTAFVTLHVGAGT
                     FQPIRTENVAEHKMHSEWFEIPPATVELVNATRARGGKVTAVGTTALRALESAARGGE
                     LAAGCGDTDIFITPGYRFRVVERLLTNFHLPRTTLIMLVSAFGGSDNIKRAYQHAIEQ
                     RYRFFSYGDAMLIERREAI"
BASE COUNT          922 a         1503 c         1372 g         1011 t
ORIGIN      
        1 cctcaagacc gtgcattacg aagccacgcg agcaggactc gatccacagt tggtgctggg
       61 gctgatccag gtggaaagcg gcttcaacaa atatgccgtg tccacggttg gcgcgcgcgg
      121 gttcatgcag gtgatgccgt tctgggtcaa atccatcggc gcgcccggcc agaacctgtt
      181 tcacctgcgc gccaacctgc gctacggctg cactattctg cgccattatc tggacatcga
      241 aaatggcgat ttgtaccgcg cactcgggcg ctacaacggc agcgtgggaa aacctgaata
      301 tcccaacctg gtggtggggg cctggaagcg ccactgggca tactctccag tgcgatcata
      361 ataatcggtt gttcggtcgc gatattctgc cgcactgcca tggtgcaatt tcaattgcac
      421 accacatcaa tccggtctaa catcccccga ggcaggtcag gcgctattga atgggcacag
      481 acccatgccc tgccggtttt gcaaacacag atgttttcca aatcgatttt tgggaggaac
      541 gtatgaatca gttaactgct ctgtcgctca gcattgctgt acttggtggt ctgtgggtgt
      601 ttctggcgct cggtccgctt tccggactgg ttctggtgtg ggcaggcttc atcgcttggg
      661 gttgttattt ccatactggc ggtgatgccg cggcattgaa aaagaccatt gccggcatga
      721 tttacggtgc catcttggcc ggagtcgcgc tgttcctgct cggcaccaat ccgctccatt
      781 tgcctgccgt tgccgctgcc ccggtatatg tagccgtgac cgtgttcttc ctggtcatcg
      841 tcgccggatg caacctgctg tccgtagtgc cggccaatgt ttacggctat gccgcaactg
      901 tagcctatgc agtgcaaacc gccaacgccc ctctggtgac ggatttgatc atggcaaacc
      961 cggtgatcgt gctgtcgctg tcctttgtgc tcggtgccgt tctcggcatg ctatccggca
     1021 aactggcggg cgtactgggc aagtagcctt gggctggatt tgtcacgctg catcagcgtg
     1081 acaagccaac ggcggacaag gtgtccgccg tttttttatt tagtccggat ttggaatgcc
     1141 ttcagtgagg cagttccctt tgcaccgccc gatagaagcg ttcgtaaaaa tcccgcttgg
     1201 tcaccgtctc cccccggatt ttcacctggt tgtcggcctc gctactggtg ccgaaggata
     1261 tgggcgccag caaagggatc ccgatcagag tgctctcgct gctggctttg acgtcgaaat
     1321 gctcttccgt agcggtcacg aacaaggtag aaccacctgc acgctgctca cagctgacat
     1381 atacctgcag tatggcgtga attttctctt cgccctggaa ttccttgcca ccggaaaggc
     1441 tttgaccttc tcttttcgta acaacgtagc catcgcttaa caatgcccgt cgggcggcgt
     1501 cgcacaaagg cgcgacggcc acctggaagt ccctgcgata tctggagtcg gactggaaac
     1561 cctcggtttc agcatagccg cgcgggccgc cacaggacgc cagaagcaga gggattaata
     1621 tcaacgataa ccttttgcac ctcatcttgc ccctcctgct ggagtaatcc gtcgcttcgc
     1681 gaaccggtga tcggcagaga aacagtatca gcaaggcctc tgacaaacaa gacgtcgata
     1741 cggattccaa ccgtcaaatc agatatcttt agggcgcctc taaaaattca agtttctaag
     1801 ccagacaagg cgcgagcgaa aaattatgac gccgcatatg tcggatatgt aaggaatcat
     1861 tttttgtgag caacgccgta tggcgacgaa aattggattt ttagaggtgc cctttattac
     1921 ttggcctggt ccgaaatcct gaagccgtaa taggtaccga attcgccttc agactcatga
     1981 tagacgaaga acatgccgtt ggcgtaacca tgcccactgt agtgattgtt ggcacgcaac
     2041 ttcatggcgg aaggcaaggc gctcctacca acgaacttgc ctttcaggtc gaacaccaga
     2101 accgccttgt gatcgacatc cagcacagcc agttcctcac cgggaatccc ggtaaatgca
     2161 acgtaggagt cgctgatgtc atcgaagcgc gctccggccg cggccaagtc gagcttgatc
     2221 tctctgaccg gctcgcgttt gtccagcttg accaccagca cccggtctga acgcccctgt
     2281 ttggcgtaat actggttctc cgccgggtcg taggtcggaa aggtggcagc atcgccaaac
     2341 gccgggttgg gcccgctgag catatcggag ttgttagtca gcttgccttc cttgtccagt
     2401 tcaaggctat acacaccgca cagtggcaac atccccacgt cgctggagac attgtaggta
     2461 accgtttcca gccggttctg gttagggttg aagtagaccg tcttgttgtt cagtcccggc
     2521 ttggcgctgc tgacgtagtt gcctttggca tcataaacgt ggatcagcga tttcgaggcg
     2581 atgttgccgt attcatccgc cattgcggca aaaccgccat ccgcgataat gtagcgcgcc
     2641 tgctccggca cgtaggccac cgtcatcgga cgcgaaaccg gccgcgccgc cagcggaatg
     2701 cgcatgcctt ccagcgcttc cgccaacacc tgcgccccgg ctggcagact taaaatcatg
     2761 gccagcgccc ccgctattgc caatctccat cgtttgtcgt tcatactgtt tcctcgatca
     2821 gtgaaagtgg agattttacc tgctacagcc tatggacaaa cacccgcacc tgttagtttt
     2881 catctcctcg catggcttcg gccatatcgc gcaaaccgcg ccggtgctca acgccctgcg
     2941 caagctcatc cccggcctcg cgctcactat ccgctccacc gcacccctcg gtcagttgcg
     3001 ctcgcgcatc cacggcgagt tcgaatacct gccgctggcg ggcgacatcg gcatgctcat
     3061 gtcctcggcg ctggacgtgc gcgcggcgga aagcgctgcc gcttaccgcg agctgcatgg
     3121 cgcatgggaa gcacgggtgg caagcgaagc gaccttgctg cgcgacatcg cgccggattt
     3181 cgtgctctcc aacatcggct acctgccgct ggccggggcg caacgcgcgg gaattccttg
     3241 cgccgcaatg tgctcgctca actgggcgga cattcattca cactactgcc aggatcccgc
     3301 aatcaccgca cagatccaca gggcctacgc cggagcccaa gcgtttttgc gactcacccc
     3361 cggcatggct atggttgacc tgcccaaccg tatcctgatt gacgccgtgg ctgaggtcgg
     3421 tcgcaaccgg cgcgaagaaa tcaaccatcg ccttggcttg ggatacgagg acaaactggt
     3481 gctggtcagc ctgggcggca tcagcggtcg cctgcccatc gaaacctggc cgcgccagcc
     3541 tggcgtacgc tggttggtgc agaccgactg gcgcgccgaa caccccaacg cggtggcgct
     3601 ggaatcactg caaatggaat tcagcgacct gctcgccagc agcgacgcgc tggtctgcaa
     3661 accaggctat ggcagcttcg tcgaagccgc ctgcagcggc gtgccagcgc tgtttgccag
     3721 ccgcgccgac tggccggaaa gttccgccct caccgactgg ctgaaagaaa acggtctgtg
     3781 ccgcgaagtc agccggcaag cgctggaagg tggcgatttc gccaccgaac tccaggccat
     3841 gtgggatgcc acccgcccag aacctgtgca cccctccggt atcgcccagg ccgccgactg
     3901 gctggccgcc aggctcctgc ctagatagcc tcccgccgct cgatcagcat ggcgtcgcca
     3961 tagctgaaaa agcggtagcg ctgctcgatg gcgtgctgat aggcgcgctt gatgttgtcc
     4021 gagccgccga acgcggacac cagcatgatg agcgtggtgc gcggcaggtg gaaattggtc
     4081 agcagccgct ctaccacgcg gaagcggtag ccgggggtga tgaaaatgtc agtatcgcca
     4141 cagccggcag ccagttcgcc gccacgtgcc gcgctttcca gcgcgcgcag cgccgtggtg
     4201 cccaccgccg tgaccttgcc accgcgcgcc cgggtggcat tgaccagttc caccgtcgcc
     4261 ggtggaatct cgaaccactc gctgtgcatc ttgtgttccg ccacgttctc ggtacggatc
     4321 ggctggaagg tgccggcgcc gacgtgcagg gtgacgaacg cagtgctcac ccctattccg
     4381 gtaagttgcg ccagtagcgc cgcgtcgaaa tgcagcccgg cggtgggtgc ggcgacggcg
     4441 cccggctggc gcgcatacac cgtctggtag cgctcctcgt cagtgtcgtc cgccgcgtgg
     4501 gtgatatagg gcggcagcgg caggctgccg tggcgctcca gcaaatccag caatgccttg
     4561 tcgccggcaa agccgagttc gtacaaatcg tcctgccgcg ccagcacctc gacctcgatg
     4621 gcgtcgccag ccagccacag ccgcgccccc ggcctaggcg cgtgcgaagc gcggatgtgc
     4681 gcgaggcaac gcttgtcatc cagcacgcgc tccaccagca cctcgatttt tccgccgctc
     4741 tccttgcggc cgttcaaccg cgccttaagc acgcgcgtat cgttgaacac cagcaggtca
     4801 ctaggctg
//
LOCUS       DAOMFA010000121         4791 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_2371_length_4791_cov_5.080659, whole genome shotgun sequence.
ACCESSION   DAOMFA010000121 DAOMFA010000000
VERSION     DAOMFA010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 4791)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4791)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4791
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2371_length_4791_cov_5.080659"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..400
                     /locus_tag="PKW44_06685"
     CDS             <1..400
                     /locus_tag="PKW44_06685"
                     /inference="COORDINATES: protein motif:HMM:NF021621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="host attachment protein"
                     /protein_id="HQR51305.1"
                     /translation="RLLEQECLVNLQQEASGKELWSDNSGGNRSTGGGGSHGYDDHRG
                     QHRAEYERRYAQQVAAATTQLARERKANSVVLAADSRLLGLMREELHRANGFDLKPVS
                     RDYSKLSALELHEQLAGLDLLPARRKPGGQ"
     gene            390..1136
                     /locus_tag="PKW44_06690"
     CDS             390..1136
                     /locus_tag="PKW44_06690"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HQR51306.1"
                     /translation="MASKRLAGLLALLGAMAMRGVLADEIVEREYAQRRAAMVEEVSE
                     MAREVAPSYGGTPLSPEVLEAMSRVPRHLFVPQQEQRYAYQNRPLSIGHGQTISQPYI
                     VALMSDLLAVKAGGRVLEVGTGCGYQAAVLAEMGVEVYSIEIVEPLARQAAETLQGAG
                     YGRVRVMAGDGYLGWPEHAPFDGVIVTAGASHVPQPLLDQLKPGGRMVIPLGEAYNVQ
                     DLLLIRKGENGDTHSQNVIPVRFVPLTGGH"
     gene            complement(1282..2577)
                     /locus_tag="PKW44_06695"
     CDS             complement(1282..2577)
                     /locus_tag="PKW44_06695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR51307.1"
                     /translation="MYRRVPLNILRASVVLCFPLTALAATNDDVEQLRQQMQDLQQRY
                     EAQNATLRSMAARLQQIEAGKGGQPRYVKTGGATSNAPVGEEAGSPDAAGAARGTAQA
                     AAGEEKVVREAPAARSAEAILLEQRALFQQTFSIEPGISYSHTDRRDLSLNGFLALDA
                     IFLGNINLDRIKSDTFTADVTARYGPTDRLQFDVNIPYLYRDTNFASGGAAGGAAGVT
                     SDHSVTDNDIGDISAGVYYSLFQESPGNPDTVISLRAKAPTGKDPYGIKLKQDPSNTN
                     LYYPTELPTGNGVWALTAGISFIRSLDPAVVFANLGYTYNIIESFNDISSTPGTQTSA
                     KVDLGNHYTLGAGIAFALNERMSLSMSYSQRITDTVRIKEKGQSWQDVSGSDTNSGAL
                     NFGVTYGLTDKLSALTSVAMGVTPDAPDVVVGIRFPYRF"
     gene            complement(2781..3533)
                     /locus_tag="PKW44_06700"
     CDS             complement(2781..3533)
                     /locus_tag="PKW44_06700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR51308.1"
                     /translation="MTATANAGLPPDLGVFDQAEELPDAALGHMRGKFVSSGQVMYFG
                     VEMVTRWQTAAGEVIRAGAEFGVNFAGHTPRVEFRPTITAEHNGTPSLQTAQGNSMAS
                     GAGGLDNVSGVVQNIQVAGTSNGVGNTIGMNVEMLSSPSAAPASAPAANGPETLSVQT
                     ASGTTATVSMAGNGIGVAVTVPDQGRAVQQIRNVAQGGGQVLQSVQLGGNLNQIHNLI
                     NLNVKMDATNGLSSHSAQVLSGLRMLPQSGTF"
     gene            complement(3724..4467)
                     /locus_tag="PKW44_06705"
     CDS             complement(3724..4467)
                     /locus_tag="PKW44_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019896804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HQR51309.1"
                     /translation="MFEYTQREDAMIGRNCKIACCSGLLTLLLGGFAFQASAGQAQLG
                     NVIPGAGVINKNLVSMRELHFVDMVPQQTDFSCGAAALATILRYAYRKDVTEEEVIKG
                     LLTVSDLEVVQQRGFSLLDLKNYVQTLGMRGRGYNMKPEMLEQIKIPTIALLDIRGYK
                     HFVVVKKTTEDKVYLADPALGNRIMNKGDFVAGWNQVIFAVIGQGFDRESILMRPKEP
                     LTARNIVDTWRPLTDSQLAEFGFNRGEFF"
     gene            complement(4515..>4791)
                     /locus_tag="PKW44_06710"
     CDS             complement(4515..>4791)
                     /locus_tag="PKW44_06710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR51310.1"
                     /translation="NGTYHGKEGGTLGFVEASAIGLSSTFSGVVTNVETLFRPHVNNA
                     SVSGNVLAHASGNIGVNVAAGTNNLQANHLAIAAALGNGGGGTGGGE"
BASE COUNT          922 a         1447 c         1467 g          955 t
ORIGIN      
        1 gcgcctgctg gaacaggaat gcctggtcaa tctgcagcag gaggcttccg gcaaggagct
       61 gtggtcggac aacagcggcg gcaaccgcag cacgggcggt ggcggttcgc acggctacga
      121 cgaccatcgc ggccagcacc gtgcggaata cgagcggcgt tatgcccagc aggttgcggc
      181 ggccaccacg caactggcgc gcgaacgcaa agccaacagc gtggtgctgg cggccgacag
      241 ccgcttgctg ggcttgatgc gcgaggaact gcatcgcgcc aacggcttcg atctgaagcc
      301 ggtttccagg gactacagca agctttccgc cctggaactg cacgagcagc tcgccggcct
      361 cgacctgctg ccggcaagac gcaagcctgg tggccagtaa aaggctggcc ggactgctgg
      421 cgctgctggg ggctatggcg atgcgtggcg tattggccga cgagatcgtg gagcgggagt
      481 acgctcagcg tcgtgcggcg atggtggaag aggtgtccga aatggcccgg gaagtcgccc
      541 caagctacgg cggcacgccg ttgtcgccgg aggtgctgga ggcgatgtcg cgcgtacccc
      601 gccacctgtt cgttccgcag caggagcagc gctatgccta ccagaaccgg ccgctgtcca
      661 tcggccatgg ccagaccatt tcccagcctt acatcgtggc gctgatgagc gacctgctgg
      721 cggtcaaggc gggcgggcgg gtgctggaag tcggcaccgg ctgcggctac caggccgccg
      781 tgctggcaga gatgggggtc gaggtctatt ccatcgagat cgtcgagccg ctggccaggc
      841 aggcggcgga aacgctgcaa ggcgcgggct atggccgggt gcgcgtcatg gcaggggatg
      901 gctacctggg ctggccggaa catgcgccgt tcgatggcgt gatcgtcacc gccggtgcca
      961 gccatgtgcc gcaacccttg ctcgaccagc tcaaaccggg tgggcgcatg gtgatcccgc
     1021 tgggcgaggc ttacaacgtg caggacctgc tgctgatccg caaaggcgag aacggcgaca
     1081 cgcacagcca gaacgtcatt ccggtgaggt tcgtaccgct gaccggcggg cattgaagga
     1141 tgcctctaaa aatccgatag caggagcgcg tcgccatcgg cgcgatcttt cctcacccca
     1201 tgcctctccc gaaggtatcg ggacgaaaaa aatgccaacc gcaaccggtt ggcatttttg
     1261 tgcgcgagat gagcgcgttt gtcagaagcg atacgggaac ctgatgccca ccacgacatc
     1321 cggcgcatcg ggggtgacgc ccatcgccac gctcgtcagc gcggaaagct tgtccgtcag
     1381 gccataggtt acgccgaaat taagcgcgcc ggaattcgtg tcgctcccac tgacatcctg
     1441 ccagctctgc cccttctcct tgatacggac ggtgtcggtg atgcgttgcg agtaggacat
     1501 gctcaagctc atgcgctcgt tcagggcgaa tgcaatgccg gcacccagag tgtaatggtt
     1561 gcccaggtca actttggcgg aggtctgtgt gcctggggtc gaactgatat cgttaaagga
     1621 ttcgatgatg ttgtaggtgt agcccaggtt cgcaaagaca accgccgggt ccagcgacct
     1681 gataaagctg atcccggcgg taagcgccca gaccccgttg ccggtgggca gttcggtcgg
     1741 gtaatacaga ttcgtgttac tgggatcctg cttgagtttg atcccatagg gatccttccc
     1801 ggtcggtgct ttcgcgcgca ggctgatgac ggtgtcgggg ttgcccggcg attcctggaa
     1861 caggctgtaa tagacgcccg cgctgatatc gccgatatcg ttgtccgtaa cggaatgatc
     1921 ggacgttaca ccagcggccc cgccggcggc tccgccggat gcaaaattcg tatcgcggta
     1981 cagataaggg atatttacat cgaactgcaa gcgatccgtc ggcccatagc gggcggtgac
     2041 gtcggcggta aaggtatccg acttgatgcg gtcgaggttg atgttgccca ggaaaatggc
     2101 atccagggca agaaagccgt tcaaggaaag atcgcggcga tcagtatgcg aatagctgat
     2161 gcccggctcg atggagaagg tctgctggaa cagggcgcgc tgctccagca ggatagcttc
     2221 ggcacttcta gcggccggcg cttccctgac cactttttcc tcgccagcgg cagcttgcgc
     2281 cgtgcctcgt gcagcacctg ccgcgtccgg agagcctgcc tcttcgccga ccggagcgtt
     2341 gctggtggcg ccacccgtct tcacataacg cggctggcca cccttgccgg cctcgatctg
     2401 ctgcaggcgg gccgccatgg agcgcagcgt ggcgttctgg gcctcgtagc gttgctggag
     2461 atcctgcatc tgctggcgca actgctcgac atcgtcattg gttgccgcaa gggcggtgag
     2521 gggaaaacag aggacgacag acgctctgag aatatttaga ggaacgcgac gatacatgaa
     2581 acccccttgg tcaatcgctg atacagcttg aattgtgcgg ctatcatgcc caaataatcg
     2641 cttcgggtca aacggatacc ggggtttttg ttgtataaaa cacacagtcc ttcaatatca
     2701 gccacgcaga ccaagggtgg tgagattggc acactataaa tcaagggcgg gagagaatgc
     2761 cgcccttgat cgcgccggac tcagaaagtg ccgctctgcg gcaacattct caaccccgac
     2821 aacacctgtg cgctatggga cgagaggcca ttcgtggcat ccatcttgac gttcagatta
     2881 atcaggttgt gaatctggtt caggttgccg cccagttgca cggattgcag cacttgtccg
     2941 cccccctgcg ccacgttgcg aatctgctga acggcccgtc cctggtcggg tacggtgact
     3001 gccacgccaa tgccgttgcc tgccatgctc actgtcgccg tcgtgccgct ggccgtttgc
     3061 actgacaggg tctccggccc attcgccgcc ggcgctgatg caggtgcggc cgatggggag
     3121 gatagcatct cgacattcat gccgatggta ttgcccacgc cattggacgt gcctgccacc
     3181 tgaatgttct gcacgacccc gctcacatta tcaagcccgc cggcgccact ggccatcgag
     3241 ttgccctgcg ctgtctgcaa ggagggcgtg ccgttgtgct ccgcggtgat ggtcggccgg
     3301 aactctaccc gtggggtgtg accggcaaaa ttgaccccga attcggcccc ggccctgatc
     3361 acctcgcctg cggcagtctg ccagcgggtg accatttcca cgccgaagta catgacctgc
     3421 ccggacgata cgaatttgcc gcgcatatgg cccagcgcgg catctggcaa ctcttccgcc
     3481 tggtcgaaca cgcccaggtc gggcggcagg ccggcgttgg cggtcgctgt catcgacaat
     3541 gcacctatca atgccattgc cagcggtttc ctgctccagc gccgcagctt gctgctcgta
     3601 cttaacttac tatgtaaccg cgcgacttcc tgcaaagaat taagttccat gacgtactcc
     3661 tcactgacct tgtgagccgg gagcgcaacc gcgcaaggca ctccctgccc aatgtgcgag
     3721 agttcagaaa aactctccac ggttaaaccc gaattccgcc aactgtgaat cggtgagcgg
     3781 ccgccaggta tctacaatat tcctggcagt gagcggctcc ttgggccgca tcagaatcga
     3841 ctcgcgatcg aagccctgtc cgatgaccgc aaagatcacc tgattccagc cggcgacaaa
     3901 atcccctttg ttcatgatcc ggttacccag tgccggatcg gcgagataca ccttgtcctc
     3961 cgtcgttttc ttgacgacga caaagtgctt gtaacccctg atatcgagca acgcgatggt
     4021 gggaatcttg atctgctcga gcatctcggg cttcatgttg tagcccctgc cgcgcattcc
     4081 cagcgtttgt acatagttct tgagatccag cagggagaac cccctttgct ggaccacctc
     4141 caggtcgctg accgtgagca ggcctttgat cacttcttct tcggtcacat ctttccggta
     4201 ggcatatctg aggatggtgg ccagtgcggc tgcaccgcaa ctgaagtcgg tctgctgtgg
     4261 caccatgtcc acgaaatgca gttcccgcat gctcaccagg ttcttgttga tgaccccggc
     4321 gcccgggatg acgttgccca gttgcgcctg tcctgccgag gcctgaaacg caaaaccacc
     4381 cagcagcagg gtgagtagtc cgctacaaca agctattttg cagttgcgac cgatcatggc
     4441 atcctcccgt tgggtgtatt caaacatcga aatctccggc cggagaaccc ggccggagaa
     4501 tcgttcagca gcgattattc accgccgccg gtaccgccgc cgccattacc cagtgccgct
     4561 gcaatggcga gatggttggc ttgcaggtta ttggtacctg ccgccacgtt caccccgata
     4621 ttgccggaag catgggccag aacgttgccg ctcacgctgg cattgttcac gtgcggacgg
     4681 aacagcgttt cgacgttggt aaccacgccg gagaaggtgc tgctgagtcc gatcgcagaa
     4741 gcttcgacga agccgagcgt gccaccttcc ttgccgtggt aggtaccatt t
//
LOCUS       DAOMFA010000122         4772 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_2388_length_4772_cov_5.894424, whole genome shotgun sequence.
ACCESSION   DAOMFA010000122 DAOMFA010000000
VERSION     DAOMFA010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 4772)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4772)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4772
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2388_length_4772_cov_5.894424"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            100..4713
                     /locus_tag="PKW44_06715"
     CDS             100..4713
                     /locus_tag="PKW44_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HQR51311.1"
                     /translation="MPAKQGLYDPANEKDACGVGFVAHIKGRKSHDIVSKGLEILRNL
                     THRGATGYDPKLGDGAGILIQLPDAFLRKEAAKLNIALPEVGQYACGTVFLPQSANGR
                     VACESAIARIIHEEGQNFLGWRDVPRDNRNIANAARAVEPVMRQAFIGSNAADQNAFE
                     RKLFVIRKRIEHAVRALKLEDASQFYIPSLSSRTLVYKGMLLAEEVGTYYQDLRDESM
                     VSALALVHQRFSTNTFPAWDLAHPFRMIAHNGEINTMRGNVNWMAARHEAMKSAVLGE
                     DLDKLWPLIAEGQSDSACFDNALELLVAGGYSLPHAMMMLIPEAWASNPLMDAERRAF
                     YEYHAALMEPWDGPAAVAFTDGRMIGATLDRNGLRPARYLITDDDYVMMASEMGVLTF
                     PEEKIIKKWRLQPGRMFLIDLEQGRIIDDEEVKRELAQAKPYHQWIEQSRYFLDDLPK
                     TECKLDLKADLLDAQQAFGYSQEDLKFILTPMAANGEEATGSMGTDSALPVLSDKSKT
                     LYSYFKQLFAQVTNPPIDPIREELVMSLVTFIGPKPNLLGGFESTPETSPPLRLEASQ
                     PVLTPDELAQLQHIDRLTQGRFRSLVADITYAASAGTKGMDDAIEALCKAAEKSVLDG
                     YNVLILSDRNVNRDHIAIPALLATAAVHHHLVKAGLRTSTGLVVDTGSAREVHHFALL
                     AGYGAEAVCPWLAFETIANLGLADAKAAQKNFVKAIGKGLYKVMSKMGISTYQSYCGA
                     QIFDAVGLNSAFVEKYFPGTSSNVEGIGLAQVAEESLRQHQAAFGDDPVLANALDAGG
                     EYAFRVRGEEHLWTPDSIAKLQNATRTGKYETYKEYAKLINDQSKRHLTLRGLFEIKP
                     AGAPVPLDEVESAKDIVKRFATGAMSLGSISTEAHTTLAIAMNRIGGKSNTGEGGEDP
                     VRFMPVAKDTTLSELIGKSRVEADIHLKAGDSMRSKIKQVASGRFGVTAEYLASADQI
                     QIKMAQGAKPGEGGQLPGGKVSEYIAKLRFSVPGVGLISPPPHHDIYSIEDLAQLIHD
                     LKNANPKASISVKLVSETGVGTVAAGVAKAKSDHIVIAGFDGGTGASPISSIKHAGSP
                     WEIGLAETQQTLVLNQLRGRVAVQVDGQMKTGRDVVIGALLGADEFGFATAPLVVEGC
                     IMMRKCHLNTCPVGVATQDPVLRKKFTGQPEHVVNYFFFVAEEVREAMAQMGIRKFSD
                     LIGRADLLDMRAGIDHWKASGLDFSKIFYQPKVGGNVARSHCEEQDHGLDKALDNKLI
                     AQAGAALERGEKVVIETPITNTNRTAGTMLSSAVANRYGQAGLPDNTITVKLSGTAGQ
                     SFAAFLTKGITFELTGEGNDYVGKGLCGGRIVIKPPKAFRGIPHDNIIAGNTVMYGAT
                     SGESYFNGVAGERFCVRNSGASAVVEGVGNHGCEYMTGGTVVVLGQTGLNFAAGMSGG
                     VAYVLDEDGLFAKRCNLGMVSLEKVEHVDSVSGDAVHHLDQPDEITLKTLIEKHAAYT
                     GSARAITILANWDEYRARFVKVMPNEYKRALIELSEQKLLEAA"
BASE COUNT         1034 a         1468 c         1448 g          822 t
ORIGIN      
        1 gttagcggct aagcgtagcg taaacaccct caatttacgt tgcgcacgac ggttttgtcg
       61 aacaggtggc aaaggatata caggacaggg gcaacgatca tgccagcaaa acagggttta
      121 tacgatccgg caaacgaaaa agatgcttgc ggggtcggtt ttgtagcaca catcaaaggg
      181 cggaagagcc acgatatcgt cagcaagggt ctggagatcc tgcgcaacct gacccatcgc
      241 ggcgcaacag gttacgaccc caagctgggt gacggtgccg gcatcctgat tcagctccca
      301 gacgcttttc tgcgcaagga agcggccaaa ctgaacattg cattacccga agtaggccag
      361 tatgcctgcg gcacggtgtt cctgccgcaa tccgccaacg gccgcgtcgc ctgcgaatcc
      421 gccatcgcgc gcatcatcca tgaagaaggc cagaatttcc ttggctggcg cgacgtgcca
      481 cgcgacaacc gcaatatcgc gaacgccgcc agggcggtcg aacccgtcat gcgccaggca
      541 ttcatcggca gcaacgctgc cgaccagaac gcctttgagc gcaagctgtt tgtcatccgc
      601 aaacgcatcg agcacgcggt gcgcgcgctg aaactggaag atgccagtca gttctacatc
      661 ccgtcactgt ccagccgcac ccttgtctac aagggcatgc tgctggcgga agaagtcggc
      721 acttactatc aggatttgcg tgacgaaagc atggtgtccg cactcgcatt ggttcaccag
      781 cgcttctcca ccaacacctt cccggcctgg gatctggcac acccgttccg catgatcgcc
      841 cacaacggcg aaatcaacac catgcgcggc aacgtcaact ggatggcggc gcgccacgaa
      901 gcgatgaagt cggccgtgct gggcgaagat ctggacaagc tgtggccgct gatcgcagag
      961 ggccagtccg actccgcctg cttcgacaat gcgctggaat tgctggtcgc cggcggctat
     1021 tcgctgccgc acgcgatgat gatgctgatt ccggaagcct gggccagcaa cccgctgatg
     1081 gatgcggagc gccgcgcctt ctacgaatac cacgccgccc tgatggagcc atgggacggc
     1141 cccgccgccg tcgctttcac cgacggccgc atgatcggcg ccacgctgga ccgcaacggc
     1201 ctgcgcccgg cgcgctacct gattaccgac gacgattacg tcatgatggc atccgaaatg
     1261 ggcgtgctaa ccttccccga ggagaagatc atcaagaaat ggcggctgca gccgggcagg
     1321 atgttcctga tcgacttgga gcagggccgc atcattgacg acgaggaagt gaagcgcgaa
     1381 ctcgcgcagg ccaagcccta tcatcaatgg atcgagcaat cgcgctactt cctcgacgac
     1441 ctgcccaaga ccgagtgcaa gctggacctc aaggccgatt tgctcgacgc gcagcaggct
     1501 ttcggctaca gccaggaaga tctcaagttc atcctgacgc caatggcggc caatggcgaa
     1561 gaagcgaccg gttccatggg caccgacagc gctctgccag tgttgtcgga caaatccaag
     1621 accctgtaca gctacttcaa gcaactgttc gcgcaggtga ccaatccgcc gatcgacccg
     1681 atccgcgaag agctggtgat gtcgctggtg accttcatcg gtcctaagcc caatctgctg
     1741 ggcggctttg aatcaacccc tgaaaccagt ccgccgttgc gcctggaagc cagccagccc
     1801 gtgctgacac cggacgaact ggcgcagctg cagcacatcg acagactgac tcaaggtcgc
     1861 ttccgttcgc tggtagccga catcacctat gccgcctccg ccggcaccaa gggcatggac
     1921 gacgccatcg aggcgctgtg caaggcggcc gagaagtcgg tgctcgacgg ctacaacgta
     1981 ttgatcctgt ccgaccgcaa cgtgaaccgg gatcacatcg ccattcccgc actgctggcg
     2041 accgctgccg tgcaccatca tctggtcaag gccggcctgc gcaccagcac cggactcgtg
     2101 gtcgataccg gctccgcccg cgaggtgcac cacttcgccc tgctggcagg ctatggcgct
     2161 gaagccgtgt gcccgtggct ggctttcgaa accattgcca acctgggctt ggccgatgcc
     2221 aaggccgcgc agaagaattt cgtcaaagcg atcggcaagg gtttgtacaa ggtcatgtcc
     2281 aagatgggca tttccaccta ccagtcctac tgcggcgcgc agattttcga tgccgtgggc
     2341 ttgaattcgg cttttgtaga gaagtatttc ccgggcacct ccagcaatgt ggaaggcatc
     2401 gggctggcgc aggtcgccga ggagtcgctg cgacagcacc aagccgcgtt cggcgatgac
     2461 ccggtgctgg ccaacgcgct ggatgcgggc ggtgaatatg cgttccgggt ccgtggcgaa
     2521 gaacacctgt ggacaccgga ttccatcgcc aagctacaga atgccacgcg caccggcaag
     2581 tacgaaacct acaaggaata cgccaaactc atcaacgatc agagcaagcg ccatctgacg
     2641 ctgcgcggcc tgttcgagat caagccggcc ggcgccccgg tgccgctgga cgaggtggag
     2701 tcagccaagg acatcgtcaa gcgcttcgcc accggcgcca tgtcgctcgg gtcgatctcg
     2761 accgaagcgc acaccacact ggcgattgcc atgaaccgca ttggcggcaa gtccaatacc
     2821 ggcgaaggtg gggaagaccc ggtgagattc atgccggtgg ccaaagatac gacgctgtcg
     2881 gaattgatcg gcaagagccg ggtggaagcc gacatccacc tcaaggccgg cgattccatg
     2941 cgttccaaga tcaaacaggt ggcctcgggc cgcttcggcg tgacggcgga atacctggcc
     3001 agtgccgacc agatccagat caagatggcg caaggtgcca agccgggcga aggaggtcaa
     3061 ttgccagggg gcaaggtgtc cgaatacatt gccaagctgc gcttctcggt gccgggcgta
     3121 ggtctgattt cgccaccgcc gcaccacgac atttattcca tcgaagacct ggcacagctg
     3181 attcacgacc tgaagaacgc caatcccaag gcctcgatct cggtcaagct ggtgtcggaa
     3241 accggtgtcg gcaccgtggc cgcgggcgtg gccaaggcca aatccgatca catcgtgatc
     3301 gccggcttcg acgggggtac cggcgcctcg ccgatttcct ccatcaagca tgccggcagt
     3361 ccgtgggaaa tcggtctggc tgaaacgcag cagacattgg tgctgaacca gctgcgcggc
     3421 cgcgtggcgg tacaggtgga cgggcagatg aagactggcc gtgatgtcgt tatcggcgcc
     3481 ctgctgggtg cggatgagtt cggcttcgcc accgcgccgc tggtggtcga aggctgcatc
     3541 atgatgcgca agtgccacct caatacctgt ccggtgggcg ttgccaccca ggatccggtg
     3601 ctgcgcaaga aattcaccgg tcagccggag cacgtagtga actacttctt cttcgtcgcc
     3661 gaggaagtgc gcgaagccat ggcgcagatg ggcatccgca agttcagcga cctgatcggc
     3721 cgcgccgact tgctggacat gagagccggc attgaccact ggaaggccag cggccttgac
     3781 ttcagcaaga tcttctacca gcccaaggtg ggcggcaatg tcgcgcgcag ccattgcgaa
     3841 gagcaggatc acggcctgga caaggcgctc gacaacaagc tgatcgcaca agccggggcg
     3901 gcgctggaac ggggcgagaa agtcgtgatc gaaacaccga tcaccaacac caaccgcacc
     3961 gccggcacca tgctgtccag cgccgtcgcc aaccgctacg gccaggccgg gcttccggat
     4021 aacactatta cggtcaaact cagcggtacc gctggccaga gcttcgcagc gttcctgacc
     4081 aagggcatca ccttcgaact gaccggtgaa ggcaacgact acgtcggtaa gggcctgtgc
     4141 ggcggccgca tcgtcatcaa gccgcccaaa gcgttccgcg gcataccgca cgacaacatc
     4201 atcgccggca acaccgtgat gtatggggct accagcggcg agagctactt caatggcgtg
     4261 gccggggagc gcttctgcgt gcgcaattcc ggcgccagcg cggtggtgga aggcgtgggc
     4321 aaccacggct gcgaatacat gaccggcggt accgtggtcg tgctggggca gaccggtctc
     4381 aactttgcgg ccggcatgtc cggtggcgtg gcctacgtgc tggatgaaga cggcctgttc
     4441 gccaagcgct gcaatctcgg catggtgtcg ctggagaagg tcgagcacgt ggacagcgta
     4501 tccggggatg ctgtacatca cttggatcag cccgacgaaa tcacgctgaa gacgctgatc
     4561 gaaaagcacg ccgcttacac cggcagcgcg cgcgccatta ccattctggc gaactgggat
     4621 gaataccgcg ccaggttcgt caaggtcatg ccaaacgaat acaagcgcgc tctgatcgaa
     4681 ctctccgaac aaaaactgct ggaggccgca taaatgggta agccgactgg atttatggaa
     4741 ttaaagagac tggaagaaga gtatatgtcg gt
//
LOCUS       DAOMFA010000123         4666 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_2470_length_4666_cov_4.935806, whole genome shotgun sequence.
ACCESSION   DAOMFA010000123 DAOMFA010000000
VERSION     DAOMFA010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 4666)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4666)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4666
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2470_length_4666_cov_4.935806"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..816)
                     /locus_tag="PKW44_06720"
     CDS             complement(<1..816)
                     /locus_tag="PKW44_06720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR51312.1"
                     /translation="MGRLRSCEAFHNDEAGHENFRHFLRQYKNIPVYLIVDAVEEDYR
                     LETLPHAIGQARRGLVERRLGQLYRNAKYRAASLIGREKDKRRDDIYLFQALNNADSL
                     NLWVLAIEELHAPLAGIYLLPMVSQALVNRLKLVAPDLLLTDWQGTGLRQTYFQQGRL
                     RVSRLAPAADPNQERLAELYEVETEKTRLYLLSQRLIARDTILNLLVLAPRQQGEVVC
                     RRMGVDPNIECKALDSVELAQRLGLEPQQLRAFPELLYMQVLAKGGGSVNLASQ"
     gene            complement(880..2085)
                     /locus_tag="PKW44_06725"
     CDS             complement(880..2085)
                     /locus_tag="PKW44_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HQR51313.1"
                     /translation="MPYFSYKAVDQAGHPAQGRMDAVNDVDLELRLNRMGLDLISFNL
                     AAQNRGLFSGQKVSLQDLVMFCFQMEQLARAGVPLMEGLTDLHDGTESPGFREVIGAL
                     ISEVEGGKQFSQALASHPKVFSNVFVSLVRAGEQSGRLPEVFENLASTLKWQDELISQ
                     TKRLLAYPVFLLLVVVGAITFLMIYLVPQMVTFLRNMGQELPLETKILIVVSEFFVNY
                     WWLIFSLPALVVLGLGIAIRYSAAARYRYDFVKLHLPVTGSVLQKIILARFARYFALM
                     YQAGIPILDAIKTCQDIAGNRVVADALDRVYQQINAGDSMSESFHNAGLFPPLVVRMI
                     RVGENTGVLDKSLLNISYFYDRDVKESVEKMLKMLEPALTVILGALLAMIMFAVLGPV
                     YDSMTKFKF"
     gene            complement(2091..3797)
                     /locus_tag="PKW44_06730"
     CDS             complement(2091..3797)
                     /locus_tag="PKW44_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HQR51314.1"
                     /translation="MAEQRRKLRLGELMVQQGLISQDQLRIALMEQDQNNIPLGRQLV
                     RLGFVTEAMVRDLVAHTIGQESVELSTVIADAEALKMVPEEFARRFHLLPIAYEESSK
                     TLVVAMADMFNVVAVDQLRAILGGQVQIKPVLAGEAQLEEHIDQFYGYELSVDGILRE
                     IETGEVDYQSLQGGGGGEEYTQPVVRLVGALLMDAVKRGASDIHFEPEYAFLRIRYRI
                     DGVLQQIRSLHKTYWPGIAVRLKVISGMDIAETRAPQDGRLQITPAGRLFDFRGPSHP
                     TLNGENILFRVLEREKSIIPLERIGLPDVTLEQLRLMMTRPEGILIVTGPTGSGKTTT
                     LYSLLAFINTEAVNIMTLEDPVEYPVTMMRQTSVNEVNKVDFANGIRSIMRQDPDIIL
                     VGEVRDNDTAEMAFRAAMTGHQVFTTLHTNSALGTLPRLLDIGIAPEIMAGNIIGVIA
                     QRLVRLLCSHCKEAYVPDEDECKLLGIKFKDDIQVYRHKGCKRCSYTGYRGRMAIMEL
                     LRIDGEIDAMIARRSHLDQIRAIAMEKGFVPLAEDGIRRILEGRTSLAEVARVIDLTS
                     RV"
     gene            complement(3895..>4666)
                     /locus_tag="PKW44_06735"
     CDS             complement(3895..>4666)
                     /locus_tag="PKW44_06735"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQR51315.1"
                     /translation="EEVTSNAAAITEAPAKKRVESEPPVLTEKPELAATSAASPAIGK
                     TVASVEDVSVKVTRNRTPAMVVNGSVVAAYQAYSVGDDAAAERLYRQALQSEPRNIDA
                     LLGLAAVSVRQNNADQATASYLRVLEVEPRNAAAQAGLISLTAQADPLASESRLKTML
                     AQQPDAAFLHATLGSLYAEQNQWPLAQQSYFQATRYDPGNAEYAFNLAVSLDQMGKGK
                     LALEHYQRALELLPKQGASALDRAGIETRIIQLRSAAE"
BASE COUNT          983 a         1427 c         1269 g          987 t
ORIGIN      
        1 ctgcgatgcc agattgactg accccccccc cttcgccaac acctgcatgt agagcaattc
       61 cggaaatgcc ctcagctgtt gcggttccag cccgagccgc tgcgcgagct ccactgagtc
      121 cagcgccttg cactcgatgt tagggtccac acccatgcga cggcaaacca cttcgccttg
      181 ctgccttggt gccagcacta acaggttcag aatggtgtcg cgcgcgatca ggcgctggct
      241 gagcagataa aggcgggttt tctcggtttc cacttcgtac agttccgcca gccgctcctg
      301 gttcggatcg gctgcgggtg ccaaccggct gacccgcaag cggccttgct ggaaataggt
      361 ctgccgcaag ccggtgccct gccagtccgt caacagcaga tcgggagcaa caagtttgag
      421 gcgattgacc agagcctggc ttaccatcgg cagcaggtat atcccggcca gtggcgcatg
      481 caactcttca atagccagca cccaaagatt gaggctatcg gcgttgttga gcgcctgaaa
      541 caaatagata tcgtcgcggc gcttgtcttt ttcgcgacca atcaagctgg cagcccggta
      601 tttggcgttg cgatacaact ggccaagacg ccgctccacc agacctcggc gtgcttgacc
      661 aatggcgtgc ggcaaggttt cgaggcgata gtcttcctcg accgcatcaa cgatcagata
      721 aaccgggata tttttatact ggcgcaggaa atgcctgaaa ttctcatgtc ctgcttcgtc
      781 gttgtggaac gcctcgcatg aacgcaaccg ccccatgcgc caaaaccctg cagtcaggcg
      841 attgggaaat gcagtcagaa ttatctttga aagcatgggc taaaatttga acttggtcat
      901 cgaatcatac accgggccca gcacagcaaa catgatcatg gccagcaatg cccccaggat
      961 cacggtcaac gccggctcca gcattttcag cattttttcc acagactctt tcacgtcgcg
     1021 atcgtagaag tagctgatat tcagcagaga tttgtccaac acaccggtat tttcacctac
     1081 acggatcatg cgcaccacca gaggcggaaa caatcccgca ttatggaaac tctcgctcat
     1141 gctgtcaccg gcattgatct gctggtatac ccgatccagc gcatccgcta cgacccggtt
     1201 gcccgcgatg tcctggcaag ttttgatggc gtcgagaatg gggatgccgg cctggtacat
     1261 caaggcgaaa tatctggcga aacgcgccag gatgattttt tgcagaacag acccggtgac
     1321 cggcaaatgc aattttacaa agtcgtaacg gtaacgtgcc gccgcactat acctgatggc
     1381 aatacccagc cccagcacca ccagcgccgg caaagaaaaa atcaaccacc agtaattaac
     1441 gaaaaattca gaaaccacga tcagtatctt cgtttccagc ggcaactcct gccccatgtt
     1501 gcgcaggaaa gtaaccattt gcggcaccag atagatcatc aggaaagtaa tcgctcccac
     1561 caccaccagc agcaagaaga cagggtaagc tagtagccgc ttggtctggg aaatcagttc
     1621 gtcctgccac ttcagcgtcg acgccaggtt ctcgaacacc tcgggcagcc tgccactctg
     1681 ctcgccggcc cgcaccaggc tcacgaaaac gttgctgaac actttggggt gcgaggcaag
     1741 cgcctgagaa aactgcttgc caccttcgac ctcggaaatc agggcgccga tgacttcacg
     1801 gaagcctggg ctctcagtac cgtcatgcag atcggtcagg ccttccatca agggaacgcc
     1861 tgcccgcgca agctgctcca tctggaaaca gaacatcacc agatcttgca ggctgacctt
     1921 ctgcccgctg aataaccccc ggttttgggc agcaaggttg aaactgatga gatcgagccc
     1981 catgcggttg aggcgcagtt ccagatccac atcgttcacc gcatccatcc tgccttgcgc
     2041 cgggtggccg gcctgatcaa ctgccttgta gctgaaatat ggcatgcggc ttatacccgg
     2101 ctggtcaggt caatcacgcg cgcaacctcg gccaggctgg tccggccctc gaggatgcgc
     2161 ctgataccgt cttccgccag cgggacaaac cccttttcca tggcgatggc acgtatttga
     2221 tccagatgcg aacggcgcgc gatcatcgcg tcaatctcgc cgtcgatgcg cagtagttcc
     2281 atgatggcca tgcgcccgcg gtagccggtg tagctacagc gcttgcatcc cttgtggcga
     2341 taaacctgga tatcgtcttt gaacttgatg cccagcagtt tgcattcatc ttcgtcgggc
     2401 acataagctt ccttgcaatg cgagcacagc aagcgcacca ggcgctgcgc gatcaccccg
     2461 atgatattgc ccgccatgat ttctggcgcg atgccgatgt ccagcaggcg cggcaatgtc
     2521 cccagggcag agttggtgtg caaggtagta aacacctgat ggccggtcat ggcggcgcga
     2581 aaggccattt ccgccgtatc gttgtcgcgc acttcgccca ccagaataat gtccgggtcc
     2641 tgacgcatga tggagcggat accgttggcg aaatccacct tgttgacttc gttcaccgac
     2701 gtctggcgca tcatggtgac cggatattcc accggatctt ccagggtcat gatgttgact
     2761 gcctcggtgt tgatgaacgc aagcagcgaa tacagcgtgg tggttttacc gctgccggtg
     2821 gggccggtga cgatcaggat accttccggc cgggtcatca tcaggcggag ttgctccagc
     2881 gtaacatcgg gcaaaccgat ccgctccagc ggaatgatgg acttttcccg ctccagtacc
     2941 cgaaacaaga tattctcgcc attgagcgtg ggatggctgg ggccccggaa atcgaacagg
     3001 cgccccgcag gcgtgatctg caggcgtcca tcctgggggg cgcgcgtttc cgcgatgtcc
     3061 atgccgctga tgaccttgag ccgtaccgcg attccgggcc agtaggtctt gtgcaggctg
     3121 cggatttgct gcaatacgcc atcgatacgg tagcggatgc gcagaaaagc gtattccggc
     3181 tcgaagtgga tatcggaagc gccacgcttg accgcatcca tcagcagcgc ccccaccagc
     3241 cgcaccacag gctgggtgta ttcttcgccg ccccctcccc cttgcaagct ctggtaatcg
     3301 acttcgccgg tttcgatctc gcgcaggatg ccgtcaacgg acagttcgta accgtagaac
     3361 tggtcgatgt gttcttccag ctgcgcttcg cccgccagta ccggtttgat ttgcacctgc
     3421 ccgcccaaga tggcgcgcag ctggtcgacc gccaccacat tgaacatgtc cgccatcgcc
     3481 accaccagcg tcttgctgga ctcctcatag gcgatgggga gcagatggaa acgccgcgcg
     3541 aactcttcgg gtaccatctt gagtgcttcg gcatcagcaa tgaccgtgga aagttccacg
     3601 ctttcctggc cgatcgtgtg agcgaccaga tcgcgcacca tggcctcggt gacaaatccc
     3661 agccgcacca gctggcggcc cagcgggata ttgttctggt cttgttccat gagcgcgatg
     3721 cgcagctgat cctgactgat caagccctgc tgcaccatca gctcgcccaa acgaagtttg
     3781 cgccgctgtt cggccatatt tttagttaat cacttgtctc ggacatgttt atgtatcaga
     3841 tttgcgggac agaagaaacc ctcgatgcgc aaaccatcgc gcgtcacgac agcattactc
     3901 ggccgcagat cgtagctgga tgatacgcgt ttcgatgccc gcccggtcta gagcagaagc
     3961 tccctgcttg ggcaacaatt ccaaagcgcg ctggtaatgc tccagggcaa gcttgccctt
     4021 gcccatctgg tcgaggctga ccgcaagatt gaaagcgtat tccgcgtttc cagggtcata
     4081 gcgggtggct tgaaaatagg actgctgtgc cagcggccac tggttttgct cggcatacag
     4141 gctgcctagc gtggcgtgaa gaaaagccgc atccggctgt tgcgccagca tggttttcag
     4201 tcggctttcg ctcgccagcg ggtcagcctg agcggtgaga ctgatcaaac ctgcctgtgc
     4261 ggcagcgttt ctcggctcca cttccagcac acgcaaataa ctcgccgtag cctggtcggc
     4321 attgttctgg cgcacagaaa ccgccgccag cccgagcagg gcgtcaatat tgcgcggttc
     4381 ggattgcagt gcctgacggt aaaggcgctc cgccgccgca tcgtcaccca cggaataggc
     4441 ctggtaagct gccacgacgc taccattgac taccattgcg ggtgtccggt tgcgagtgac
     4501 ctttaccgag acgtcctcca ccgacgccac cgtcttgcct attgccgggg atgccgcact
     4561 tgtagccgcc aattccggtt tttccgtcag caccggcggt tcagattcca cacgcttttt
     4621 ggccggagct tccgtaatgg cagccgcgtt cgaggtaact tcctcc
//
LOCUS       DAOMFA010000124         4637 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_2487_length_4637_cov_5.128328, whole genome shotgun sequence.
ACCESSION   DAOMFA010000124 DAOMFA010000000
VERSION     DAOMFA010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 4637)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4637)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4637
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2487_length_4637_cov_5.128328"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1475
                     /gene="groL"
                     /locus_tag="PKW44_06740"
     CDS             <1..1475
                     /gene="groL"
                     /locus_tag="PKW44_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110680.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HQR51316.1"
                     /translation="AKEIELKDKFENMGAQMVKEVASKTSDIAGDGTTTATVLAQAII
                     REGMKSVAAGMNPMDLKRGIDKAVIAAVEELKKLSKPCTTSKEIAQVGSISANSDSSI
                     GQIIADAMDKVGKEGVITVEDGSGLQNELDVVEGMQFDRGYLSPYFINNQDRQIALLD
                     NPFVLLYDKKVSNIRDLLPTLEQVAKAGRPLLIIAEDIEGEALATLVVNNIRGILKTC
                     AVKAPGFGDRRKAMLEDIAILTGGTVIAEELGLKLENVKLEDLGQAKRIEVGKENTII
                     IDGAGVEANIKSRIDQIKKQAEEATSDYDREKLQERVAKLAGGVAVIKVGATTEIEMK
                     EKKARVEDALHATRAAVEEGIVAGGGVALLRASKAIAATKGDNHDQDAGIGIVLRAIE
                     EPLRQIVTNAGAEASVVVNKVLEGSGNYGFNAANDTYGDMVEMGVLDPTKVTRYALQN
                     AASVAGLMLTTDCMVAELPKEDAPAMGGGMGDMGGMGGMM"
     gene            1879..4005
                     /locus_tag="PKW44_06745"
     CDS             1879..4005
                     /locus_tag="PKW44_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HQR51317.1"
                     /translation="MIDLLEGLNDKQLEAVTLPYQSALILAGAGSGKTRVLTTRIAWL
                     IQTGQVSPGGLLAVTFTNKAAKEMLARLSAMLPINTRGMWVGTFHGLCNRFLRAHHRE
                     ARLPALFQILDMADQLSAVKRVMKAMNVDDEKFPPKQVQAYINGAKEEGLRADHMEVY
                     DKHSEKLREIYAEYDAQCQREGVVDFAELLLRCYEVLARETALREHYQSRFKYVLVDE
                     FQDTNRLQYLWLKLLAGKDNCVFAVGDDDQSIYAFRGARVGNMLDFEQDFGVHKVIKL
                     EQNYRSHSNILDAANAIITHNRNRLGKNLWTAAGTGEPVRVFQGYNDIEEAGFIVEEV
                     KALHAEGQSLTDMALLYRSNAQSRVLEHALFSAGLPYRVYGGLRFFERAEIKHAMAYL
                     RLVANPEDDTALLRVINFPTRGIGARSLEQLQEAAKLANGSLWTAAEAAAAKGDGGRK
                     GIPGFVALVKHLQEQCSGLTLPEMIDVVTAQSGLRQHYQNEKEGEDRIANLDELVSAA
                     TAFINENDSSALVDFLTFASLEAGEHQADPGREALQLMTVHASKGLEFHSVFIVGVEE
                     GLFPHEQSMYESNGLEEERRLMYVAVTRARRRLYLTHAQQRMLHGQTRYGVPSRFLDE
                     IPEQLLKHLNTRAALKFSEPAYSAPCNPGAQHPWRIGQAVMHQKFGPGVVVSYEGNAD
                     DLRVQVNFGHAGMKWLALEYAKLEPI"
     gene            4073..>4637
                     /locus_tag="PKW44_06750"
     CDS             4073..>4637
                     /locus_tag="PKW44_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tim44 domain-containing protein"
                     /protein_id="HQR51318.1"
                     /translation="MKRLLMMLTVAFICLSLFSTVAEAKRFGGGSSFGKQRAAPTKQV
                     LQAPAPAPAAPAPGGNRWLGPLAGLAIGAGLASMFAGEGLGGSMGPILMALLVVGVLM
                     FLFRRFSPPRRPAMQYAGNAAPYQEPVPVQPPVYGGSATPLPATGNIPHDFPTAQFLR
                     NAKTSFIRLQAANDRKDLDDIREYTTPE"
BASE COUNT         1022 a         1353 c         1411 g          851 t
ORIGIN      
        1 tcgcgaaaga gattgagctg aaggacaagt tcgagaacat gggcgcgcag atggtgaagg
       61 aagtcgcttc caagacctcc gacatcgccg gtgacggcac caccaccgct accgtgctgg
      121 cacaggccat catccgcgaa ggcatgaagt ccgtggccgc cggcatgaac ccgatggatc
      181 tgaagcgcgg catcgacaag gccgtgatcg ctgcggtgga agagctgaag aagctgtcca
      241 agccctgcac caccagcaag gaaatcgccc aggtcggttc tatttccgcc aactccgatt
      301 cttccatcgg ccagatcatc gctgacgcca tggacaaggt cggcaaggaa ggcgtgatca
      361 ccgtggaaga cggttccggt ctgcaaaacg agctggatgt cgtcgaaggc atgcagttcg
      421 accgcggtta tctgtctcca tatttcatca acaaccagga ccgtcagatc gctttgctgg
      481 ataacccgtt cgtgctgttg tacgacaaaa aagtttccaa tatccgcgac ctgctgccga
      541 cgctggaaca agtcgccaag gccggccgtc cgctgctgat catcgccgag gacatcgaag
      601 gcgaagcgct ggccaccctg gtggtcaaca acatccgcgg catcctcaag acctgtgccg
      661 ttaaggcgcc gggtttcggc gaccgccgta aggccatgct ggaagatatc gccatcctga
      721 ccggcggcac cgtgatcgcc gaggaactgg gcctgaagct ggaaaacgtc aagctggaag
      781 atctgggcca ggccaagcgc atcgaagtgg gcaaggaaaa caccatcatc atcgacggtg
      841 ccggtgttga agccaacatc aagtcccgta tcgaccagat caagaagcag gccgaagaag
      901 cgaccagcga ctacgaccgc gaaaagctcc aagagcgcgt ggccaagcta gccggcggtg
      961 tggccgtgat caaggttggt gccaccaccg aaatcgagat gaaggaaaag aaagcgcgcg
     1021 tggaagatgc gctgcacgca acccgtgccg cggttgaaga aggtatcgtg gccggtggcg
     1081 gcgtggctct gctgcgtgcc agcaaggcga tcgctgctac caagggcgat aaccacgacc
     1141 aggatgctgg gatcggtatc gtgctgcgcg ccatcgagga gccgctgcgc cagatcgtca
     1201 ccaacgccgg cgccgaagct tcggtggtgg tcaacaaggt gctggaaggc tccggcaact
     1261 acggcttcaa cgccgccaac gatacctatg gcgacatggt ggagatgggc gtgctggatc
     1321 cgaccaaggt gacccgctac gcgctgcaga acgctgcatc cgtcgccggc ctgatgctga
     1381 ccaccgactg catggtggcc gagctgccga aggaagatgc cccggcaatg ggtggcggca
     1441 tgggcgatat gggtggcatg ggcggcatga tgtaagtaag cccgggtaac ccagatatgc
     1501 cttaggaaaa agccccgctt tgcggggctt ttttattgat atcaacaagt tattaaattt
     1561 cagtgaaaat agttcacttg ccctgtttgc gaagggcaaa acttgggcat atacttgccc
     1621 cagtctttct ttccggttga acagcctgcg tttacgacag gcaaaaagaa gccccggtta
     1681 aaggggatta aaaccggggc gtaagatgcc ttatcgagat aaggcgcccg ctcagggagg
     1741 agaagcggga gacggttaaa acgtctacga ataagatttg caccaagcaa attagttccc
     1801 gctgatatta agaaaattta atgcgtgcgc ctttgaggcg gcacgcattt tttatttgta
     1861 cataaatgaa atgtgcccat gatagatctc cttgaaggcc tcaacgacaa gcaactcgaa
     1921 gccgtcacgc tgccttacca gtccgccctg atcctggccg gtgccggcag tggaaaaaca
     1981 cgggttctga caacccgcat cgcctggctg atccagaccg ggcaggtcag tcccggtggc
     2041 ctgcttgccg tcactttcac taacaaggcg gccaaggaaa tgctggcgcg cctgtccgcc
     2101 atgctgccaa tcaacacccg cggcatgtgg gtcgggactt tccatggcct gtgcaatcgc
     2161 ttcctgcgcg cccatcaccg cgaggcgcgc ctgccggcgt tgttccagat tctggacatg
     2221 gccgaccagc tttctgccgt caagcgcgtg atgaaggcga tgaacgtcga tgacgaaaaa
     2281 ttcccgccaa agcaggtgca ggcctacatc aacggtgcca aggaagaggg gctgcgcgcc
     2341 gaccacatgg aggtctacga caagcactcg gaaaagctgc gagaaattta cgccgaatac
     2401 gacgcgcaat gccagcgcga aggcgtagtg gatttcgccg aactactgct gcgctgctat
     2461 gaagtactgg cgcgcgaaac tgccctgcgc gagcattatc aaagccgctt caaatacgtg
     2521 ctggtggacg agttccagga caccaaccgc ctgcaatacc tgtggctgaa actgctggcc
     2581 ggcaaggaca actgcgtgtt cgccgtaggt gatgacgacc agtcgatcta cgccttccgc
     2641 ggcgcgcgcg tcggcaacat gctggatttc gagcaggact tcggtgtgca caaggtcatc
     2701 aaactggaac agaactaccg ctcgcatagc aacattctgg atgccgccaa cgccatcatc
     2761 acccacaacc gtaaccgtct tggcaagaac ctgtggactg ctgccggtac cggcgaaccg
     2821 gtgcgcgtgt ttcagggata caacgatatc gaggaagccg gcttcatcgt tgaggaagtg
     2881 aaggcgctgc acgccgaagg ccagtcgctg accgacatgg cgctgttgta ccgttccaat
     2941 gcgcaatcgc gggtgctgga acatgcattg ttctcagctg ggctgccgta ccgggtctat
     3001 ggcggtctgc gctttttcga gcgcgccgag atcaagcatg ccatggctta cctgcggcta
     3061 gttgccaacc cggaagatga taccgcgttg ttgcgcgtca tcaactttcc tacccgcggc
     3121 atcggtgcgc gcagtctgga gcagttgcag gaagcggcga agctggccaa tggcagcctg
     3181 tggactgccg cggaagcggc cgccgcgaag ggcgatggcg gccgcaaggg gatcccaggc
     3241 ttcgtcgccc ttgtcaagca cttgcaggag caatgtagcg gcctaacatt gccggagatg
     3301 attgacgtgg tcactgcgca aagcggcctg cgccagcatt atcaaaacga aaaggaaggt
     3361 gaggaccgca tcgccaacct ggacgaactg gtcagcgccg ccaccgcttt catcaacgaa
     3421 aacgacagta gcgcgctggt ggattttctt accttcgcca gcctggaggc cggcgagcac
     3481 caagccgacc cgggacgcga ggcactgcaa ttgatgaccg tgcatgcgtc caagggtctg
     3541 gagtttcatt ccgtgttcat cgtcggcgtg gaggaagggc tgttcccgca cgagcagagc
     3601 atgtacgaat ccaacggact ggaggaggag cggcgcctga tgtacgtggc ggtgacgcgg
     3661 gcccggcgca ggctttacct gacgcatgcg cagcaacgca tgctgcatgg acagacccgc
     3721 tacggcgtgc catcgcgctt tctcgacgaa atcccggaac agttgctgaa gcatcttaat
     3781 acgcgcgctg cactgaagtt ctcggagccg gcttacagcg cgccgtgcaa ccctggcgcc
     3841 cagcatccct ggcgcattgg ccaggcggtg atgcaccaga aattcggccc gggcgtggtg
     3901 gtgagctacg aaggcaacgc cgatgacctg cgcgtgcagg tcaatttcgg ccatgccggc
     3961 atgaagtggc tggcgctgga atacgccaaa ctggagccaa tctagccggc ccatcagccg
     4021 ggaaactgtt agacttcgcc acgctttgat ttacctgccg atgaggaaac gcatgaaacg
     4081 ccttttgatg atgctaacgg ttgcttttat ttgcctgagc ctattttcca cagtagcgga
     4141 agccaagcgc tttggcggcg gcagcagttt cggcaagcaa cgcgcagcac ctacgaagca
     4201 ggtgctgcaa gcgccggccc ccgctccggc ggcgcctgcg ccaggtggta accgctggct
     4261 gggccccttg gccggcctgg ccattggcgc cggcctggcc tccatgttcg ccggggaggg
     4321 gctgggcggc agcatggggc ctatcctgat ggctctgctc gtggtcggcg tgctgatgtt
     4381 cttgttcagg cggttcagcc cgcctcggcg gcctgccatg caatatgccg gcaacgccgc
     4441 gccttatcag gagccggtcc ccgtgcagcc accggtctat ggcggcagtg ctacgccgtt
     4501 accggcaaca ggcaacatcc cgcatgattt cccgaccgcg caattcctgc gcaatgccaa
     4561 gacctcgttc atccgcctgc aggcggccaa tgaccgcaag gacttggacg acatccggga
     4621 atacaccacc ccggaag
//
LOCUS       DAOMFA010000125         4595 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_2525_length_4595_cov_6.099780, whole genome shotgun sequence.
ACCESSION   DAOMFA010000125 DAOMFA010000000
VERSION     DAOMFA010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 4595)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4595)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4595
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2525_length_4595_cov_6.099780"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1544
                     /locus_tag="PKW44_06755"
     CDS             <1..1544
                     /locus_tag="PKW44_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysine decarboxylase"
                     /protein_id="HQR51319.1"
                     /translation="FVTNGTSTSNKIVWHSTVAPNDIVVVDRNCHKSVLHSIIMTGAI
                     PVFLMPTRNHFGIIGPIPKEEFAWENIQKKIAKNPFATDKNAKPRVLTITQSTYDGVI
                     YNVEEIKEMLDGKIDTLHFDEAWLPHAAFHDFYGDYHAIGADRPRCRESMVFATQSTH
                     KLLAGLSQASQILVQDCETRKLDRVIFNEAYLMHTSTSPQYAIIASCDVAAAMMEAPG
                     GTALVEESISEALDFRRAMRKVDEEWGVDWWFKVWGPDVFSEEGVEDREAWMLKPGDR
                     WHGFGDLADGFNMLDPIKATIITPGLDVDGNFAADIGIPAAIVTKYLAEHGIIVEKCG
                     LYSFFIMFTIGITKGRWNTMVAALQQFKDDYDKNQPCWKVLPEFVTKHPRYERIGLRD
                     LCTQIHGVYKANDVARLTTDMYLSEMAPAMKPSDAFARMAHKEIDRVEIDALEGRITA
                     VLLTPYPPGIPLLIPGECFNATIVNYLRFAREFNTRFPGFETDVHGLVKSEDGKYYVD
                     CVAQP"
     gene            complement(1610..2101)
                     /locus_tag="PKW44_06760"
     CDS             complement(1610..2101)
                     /locus_tag="PKW44_06760"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HQR51320.1"
                     /translation="MARISIIAAIARNGVIGLDNTLPWHLPEDLKRFRALTMGHHILM
                     GRKTYDSLGRLLPGRTTVIVSRNPGYVVPGALVAGSLKQALQACGGDEEVFVIGGAQL
                     YHEALPLADRLYLTWIERDFEGDAHFPPLDLAQWHETGRETGISADGLGYAFVTYERE
                     ATL"
     gene            complement(2254..3012)
                     /locus_tag="PKW44_06765"
     CDS             complement(2254..3012)
                     /locus_tag="PKW44_06765"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HQR51321.1"
                     /translation="MLSPYRAKVLLALLLSLLLHTLIYATWPQLQAVSSMPVLVQGEL
                     VAPEIEEPPAPEPPPPQARPEPIPELPAKAREQKEPAPREKPDTGIALPLIAEQADDA
                     STNDYVVPQAPPLEADDKLPFASKTGTVPLEQYVPSSGAGAEPSSGNEEDAVDREVLA
                     EYGRILRDRAAQAGGYPPLAAKRGWQGRVKVLVRFSRYGLPRQIEVKESSGHKVLDER
                     ALEMVRQACAEAALPEALNRKAFSVVVPVDFKLM"
     gene            complement(3030..3446)
                     /locus_tag="PKW44_06770"
     CDS             complement(3030..3446)
                     /locus_tag="PKW44_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HQR51322.1"
                     /translation="MAIVSNENGMMSDINVTPLVDVMLVLLTVFIVTAPLLMNAVSVK
                     LPKAAATTAISKINSVRISITEDGKLYVDQNLVRGSLEAELRRMRGQGEFSVEILGDE
                     KVPYGYVARAMAQVQRAGISKFAFVMLPEKPQAAKQ"
     gene            complement(3449..4093)
                     /locus_tag="PKW44_06775"
     CDS             complement(3449..4093)
                     /locus_tag="PKW44_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020182733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HQR51323.1"
                     /translation="MDAVPDIVDAVLWLLAALSVLTWAIMFFKAYAFWADRRGNGRLL
                     EGFGRAGQVRDVRPVVAHASCTLANVGRAGLAELQALPHDASTLDIKDRREMLHHALR
                     QQIHLEQSRLENGLAVLASVGSTSPFIGLFGTVWGIMHALKAISASGSAGLDVVAGPI
                     GEALIATAMGIAAAIPAVLAYNYFLRKVRLSVAAMEHFADRFLHLAIKSGMKED"
     gene            complement(4114..4410)
                     /locus_tag="PKW44_06780"
     CDS             complement(4114..4410)
                     /locus_tag="PKW44_06780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR51324.1"
                     /translation="MTQSSIRLHDPASSARGLGSVERKIRDEIRIGEYLDAASRKAEQ
                     EVFALDASSRLTGYAQTIDSNVSRAFLYRNWRLVGLVYHNHSNGSMLLLEINYF"
     gene            complement(4382..>4595)
                     /locus_tag="PKW44_06785"
     CDS             complement(4382..>4595)
                     /locus_tag="PKW44_06785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR51325.1"
                     /translation="KHIMAVISLWLIVANNGCYQPDCWLQEKLVKIIFDKNQMDIKNK
                     IAACFLLNNKIRENLTGYDPIKHQVA"
BASE COUNT          950 a         1450 c         1286 g          909 t
ORIGIN      
        1 acttcgtcac caacggcacc tcgacttcca acaagatcgt ctggcactcc accgtcgccc
       61 ccaacgacat cgtggtggtg gatcgcaact gtcataaatc ggtgctgcac tccatcatca
      121 tgaccggcgc gatcccggtg ttcctgatgc cgacgcgcaa ccacttcggc atcatcgggc
      181 cgatcccgaa ggaagagttc gcctgggaaa acatccagaa aaagatcgcg aagaatcctt
      241 tcgccaccga caagaatgcc aaaccgcgcg tgctgaccat cacccagtcc acctacgacg
      301 gcgtgatcta taacgtcgag gaaatcaagg aaatgctgga cggcaagatc gacaccctgc
      361 atttcgacga agcctggctg ccgcatgccg ccttccacga tttctacggc gattaccacg
      421 ccatcggcgc cgatcgcccg cgctgtaggg agtcgatggt tttcgccacg cagtcgacgc
      481 acaagctgct ggccggcctc agtcaggcgt cgcagatact ggtccaggac tgcgaaacgc
      541 gcaagctcga ccgcgttatc ttcaacgagg cctacctgat gcatacctcg acctcgccgc
      601 agtacgcgat catcgcctcg tgtgacgtcg ctgcggcgat gatggaagca ccgggtggca
      661 ccgcgctggt cgaggaatcg atctccgaag ccctcgattt ccggcgcgcc atgcgcaagg
      721 tcgacgaaga gtggggcgtc gactggtggt tcaaggtctg gggtcccgac gttttttccg
      781 aggagggcgt cgaagaccgc gaagcctgga tgctcaagcc cggcgaccgc tggcacggct
      841 tcggcgatct ggccgacggc ttcaacatgc tcgacccgat caaggcgacg atcatcacgc
      901 cgggcctcga tgtcgatggc aattttgccg ccgacatcgg catcccggcc gccatcgtca
      961 ccaagtacct cgccgagcac ggcatcatcg tcgagaagtg cggcctctac agcttcttca
     1021 tcatgttcac catcggcatc accaagggcc gctggaacac catggtggcg gcgctgcaac
     1081 agttcaagga cgactacgac aagaaccagc cgtgctggaa agtgctgccg gaattcgtca
     1141 ccaagcaccc gcgctacgag cgcatcggcc tgcgcgacct gtgcacccag attcacggcg
     1201 tatacaaggc caacgacgtg gcgcgcctga ccaccgacat gtatctgtcg gaaatggccc
     1261 cggccatgaa gccttccgat gctttcgcca ggatggcgca caaggaaatt gaccgcgtgg
     1321 aaatcgacgc gctggaaggc cgcatcaccg ccgtgctgct gaccccctac ccgcccggca
     1381 ttccgctgct gattcccggc gaatgcttca acgccaccat cgtcaactac ctccggttcg
     1441 cgcgtgaatt caacacgcgc ttccccggct tcgaaaccga tgtgcacggg ctggtaaaaa
     1501 gcgaggacgg caagtactac gtggactgcg tggcacaacc gtaggagcgg cgcctcgccg
     1561 cgatagcatg gctaatcggg ccgtgggcgg cgctcctgca ccttgagccc tacaaagtcg
     1621 cctcacgctc gtacgtcacg aacgcatacc ccagcccgtc cgcgctgatg ccggtctcgc
     1681 ggccagtttc gtgccattgg gcaaggtcaa ggggagggaa gtgcgcgtca ccttcaaaat
     1741 cccgctcgat ccaggtcaga tatagcctgt ccgccagcgg cagtgcctcg tggtaaagct
     1801 gcgcgccgcc aatcacgaac acttcctcgt caccaccgca tgcctgcaat gcctgtttca
     1861 acgatccggc gacgagcgct cccggcacca cataccccgg attgcgactc acgatcaccg
     1921 tggtacgccc cggcaacaaa cgccccagag aatcataggt cttgcgcccc atgagaatgt
     1981 ggtgccccat ggtcagcgca cggaagcgct tgagatcctc cggcagatgc cagggcaggg
     2041 tattgtcgag gccgataacg ccgttgcgag cgatggcggc gatgatagag atgcgtgcca
     2101 tggcacacat tatacctccc tcgatcagga cattctccct ggtactttca ttcgccagaa
     2161 ttgccaaata acaagacgct ggaaggaagc gtttgccccc tgccgttggt ttctccacat
     2221 gagaaccgct tgcttcgatt gcaaacagag gtactacatc aatttgaaat ccaccggtac
     2281 cacaaccgaa aaggccttgc ggttcagcgc ttcgggcaga gccgcttcgg cgcacgcctg
     2341 cctgaccatt tccagcgcgc gctcgtccag caccttgtga ccgctcgact ccttgacttc
     2401 gatctgtcgc ggcaagccgt agcgggagaa tctgaccagc actttgactc tgccctgcca
     2461 accgcgcttg gccgccagtg gcggatagcc tccggcttgc gcggcccggt cgcgcaggat
     2521 gcggccgtat tcggcgagca cttcacgatc cactgcgtcc tcttcgttgc cagaagatgg
     2581 ctctgcaccg gctccggacg atgggacata ttgctccagc ggaaccgttc cggttttgct
     2641 ggcgaagggc agcttgtcgt ccgcctccag tggcggcgct tgtggcacta cgtaatcgtt
     2701 ggtactggcg tcatccgcct gttccgctat cagcggcagg gcgatgccgg tatcgggttt
     2761 ttccctgggc gcaggctctt tctgctcgcg cgccttggcg ggcagttctg gaattggctc
     2821 gggcctggcc tgcggaggcg gtggctcggg ggcagggggt tcctcgattt ctggcgcaac
     2881 cagttcgccc tgcaccagaa cgggcatgga gctcactgcc tgcagctgcg gccatgtggc
     2941 gtaaatcagt gtatgcagca gcagcgagag cagcagcgcc agcagcacct tggcgcgata
     3001 aggcgacaac atagcggcca tagataacgc tattgtttcg cggcctgcgg tttttccgga
     3061 agcatgacga aggcaaactt gctgattccg gctcgctgca cctgtgccat ggcccttgca
     3121 acgtagccgt agggcacttt ttcatccccc aggatttcca cgctgaactc gccctgtccg
     3181 cgcatgcgcc tgagttctgc ttccagactg ccgcgcacca gattctgatc gacgtaaagc
     3241 ttgccatcct cggtaatgct gatgcgcacg ctgttgatct tgctgatggc agtcgtcgcg
     3301 gcggccttgg gtagcttgac cgataccgcg ttcatcagca gcggcgcggt gacgatgaac
     3361 accgtcagca gcaccagcat cacgtccacc agcggggtca cgttgatgtc gctcatcatg
     3421 ccgttttcgt tggaaacgat cgccatgcct aatcctcctt catgccggac ttgatagcca
     3481 gatgcaggaa acggtcggcg aaatgttcca tggcggcgac cgacagccga accttgcgca
     3541 ggaaatagtt gtaggccagc accgccggaa tcgccgcggc aatacccatg gcggtggcga
     3601 tcagcgcttc gccgatgggg ccggccacca cgtcgaggcc cgccgatccc gaagcggaaa
     3661 tggccttgag cgcgtgcatg atgccccaca cggtaccgaa caggccgatg aaaggcgaag
     3721 tcgaccccac gctggcgagt accgccaggc cattctccag ccgcgattgc tcaagatgga
     3781 tctgctgccg caaggcatga tgcagcattt cacggcgatc cttgatatcc agcgtagagg
     3841 cgtcatgggg cagtgcctgc aattccgcca gccctgccct gccgacattg gccagggtac
     3901 agctggcgtg ggccacgaca ggccttacat ctcgcacctg cccggcgcgg ccgaagccct
     3961 cgagcaagcg cccgttaccg cgccgatctg cccagaaggc gtaagccttg aaaaacatga
     4021 tggcccaagt cagcaccgaa agcgcggcca gcagccataa cactgcatca acaatatcgg
     4081 gaacggcatc catggattgg tccttgcaaa aattcagaag tagtttattt ccagtaacag
     4141 catcgagccg ttgctgtggt tgtgatagac gaggccgaca agccgccagt tgcgataaag
     4201 aaacgcgcgc gacacgttgc tgtcgatggt ttgagcatag cctgtcagtc gtgatgacgc
     4261 atccagcgca aagacttctt gctcggcctt acgcgaggcg gcatccagat actcgccgat
     4321 ccggatttca tcgcggatct tgcgctcgac gcttcccagc ccgcgggccg acgatgcggg
     4381 atcatgcaac ctgatgcttg attgggtcat accctgtcag attttctcga attttattat
     4441 taagcaaaaa acatgctgct attttatttt ttatatccat ttgattctta tcaaaaatta
     4501 tttttaccag tttttcctgc agccaacaat ccggctgata acaaccatta ttggctacta
     4561 tcaaccacag gctgataaca gccattatat gttta
//
LOCUS       DAOMFA010000126         4580 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_2536_length_4580_cov_5.184530, whole genome shotgun sequence.
ACCESSION   DAOMFA010000126 DAOMFA010000000
VERSION     DAOMFA010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 4580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4580
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2536_length_4580_cov_5.184530"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..733)
                     /locus_tag="PKW44_06790"
     CDS             complement(<1..733)
                     /locus_tag="PKW44_06790"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HQR51326.1"
                     /translation="MSRPQNVTVLGSTGTIGVNTLDVIARHPDRFHAYALTANSRIEP
                     LLKQCLQFKPRYAVVLDTGAAETLRQQLRQAASETEVLCGAVALEQVSSAPEVDAVMA
                     AIVGAAGLKPAMAAARAGKRVLLANKETLVMSGRLFMEAVRKGGATLLPIDSEHNAIF
                     QVMPLQFDRSLDGNGIRKILLTASGGPFRCWSALELEQVTTEQALNHPNWVMGPKITI
                     DSSTLMNKGLEVIEAHWLFGAEADRI"
     gene            complement(730..1554)
                     /locus_tag="PKW44_06795"
     CDS             complement(730..1554)
                     /locus_tag="PKW44_06795"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HQR51327.1"
                     /translation="MLKNRLITALILGAVFLGALFLLTDLVWALFLLVFILVGAWEWG
                     GMVGYQRRGKLVFLAATALISVLLLPGMPGTMLWNVRISALLLLAATLFWVVLMPLWL
                     GHQWRIRRGLVAPVLIGWLLLLPPWIALLNLRHVSPQAVLLVVLTVALADSAAYFSGK
                     RFGRRKLAPQISPGKTWEGFFGALAGVTLFGFALCVWRSWSLWLVVGFIAIAVLSVMG
                     DLFESMAKRQAGIKDSGSIFPGHGGVLDRIDGLTSTLPLVAFCVYLPFYLSVLIPR"
     gene            complement(1547..2308)
                     /locus_tag="PKW44_06800"
     CDS             complement(1547..2308)
                     /locus_tag="PKW44_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HQR51328.1"
                     /translation="MARFASSTHDIPQSLDVPRHIAIIMDGNGRWAKKRFLPRVMGHK
                     RGVETVRDVVRRCVDMGVQYLTLFAFSSENWRRPPDEVKFLMGLFMEALKREVTRLHE
                     NQIRLVMIGDRSRFDPLLVKAIHDAEKLTQHNTRLTLTIAANYGGRWDIMQAMKRMLQ
                     ARPELAQDFTEQDLADFLSMHYAPEPDLFIRTGGEQRISNFLLWQLAYSELYYTDALW
                     PDFDDAAFKQAIQSYRQRERRFGRTSEQLVPDGHA"
     gene            complement(2377..2934)
                     /gene="frr"
                     /locus_tag="PKW44_06805"
     CDS             complement(2377..2934)
                     /gene="frr"
                     /locus_tag="PKW44_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HQR51329.1"
                     /translation="MINDVKKTAEQKMQKALEVLKADLGKVRTGRAHAGLLDQVMVEY
                     YGNMVPISQVGNVGLADARTITVQVWEKPMMAKVEKAIRDSDLGLNPANSGDMIRVPM
                     PPLTEERRRDLTKVVRAEGENAKVSVRNIRRDANDQLKKLLKDKEIGEDEERRAQDEI
                     QKLTDKFVAEVDKALQAKEADLMAI"
     gene            complement(2947..3663)
                     /gene="pyrH"
                     /locus_tag="PKW44_06810"
     CDS             complement(2947..3663)
                     /gene="pyrH"
                     /locus_tag="PKW44_06810"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HQR51330.1"
                     /translation="MTAPAFKRILLKLSGEALMGEDSYGINRATIERIVKEIKEVVDL
                     GVEVAVVIGGGNIFRGVAPAAAGMDRATADYMGMMATVMNALALQDAMRQVGLVSRVQ
                     SALTIEQVAEPYIRGKAMRYLEEGRVVIFGAGTGNPFFTTDTAAALRGMEMNADIVLK
                     ATKVDGVYTDDPKTHPDAMRYKDISFDEAIVKNLKVMDATALTLCRDQKLPICVFSIF
                     KQGALKRVVMGEDEGTRVLP"
     gene            complement(3671..4546)
                     /gene="tsf"
                     /locus_tag="PKW44_06815"
     CDS             complement(3671..4546)
                     /gene="tsf"
                     /locus_tag="PKW44_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HQR51331.1"
                     /translation="MAEITAGMVKELRERTDAPMMDCKKALSEADGDMARAEEILRVR
                     FGNKASKAAGRVAAEGSVGISISADGKTGAMIEVNSETDFCAKNDDFLAFVRDLAVVV
                     ANGKPADVAAVGGLKLGNGTVEDARTQLVGKIGENITPRRFLAVEAKGRLASYVHGGK
                     IGVLVDVVGGDEALAKDIAMHIAATKPKSLDSSGVAPELIEAERRVAIEKAREAGKPE
                     AMLDKIADGTVQKFLKEVTLLSQPFVKDDKQTIEQLLKSRGASVASFNMYVVGEGIEK
                     KVTDFAAEVAAAAKV"
BASE COUNT          922 a         1476 c         1246 g          936 t
ORIGIN      
        1 cgatacgatc ggcttcggcg ccgaacagcc agtgcgcctc aatcacctcc agccccttgt
       61 tcatcaaagt ggaggagtcg atggtgattt ttggccccat cacccaattg ggatgattca
      121 gcgcctgctc ggtcgtcacc tgctccagtt ccagagcgct ccagcaccgg aacggtccac
      181 cggaagcggt cagcagaatt ttgcggatgc cgttgccgtc caggctgcga tcgaactgaa
      241 gcggcatgac ctggaaaatg gcattgtgct cgctgtcgat cggcaacaaa gtggcgccac
      301 ccttgcgcac ggcctccatg aacaggcggc ccgacatcac cagcgtttcc ttgttggcca
      361 gcagcacgcg cttgccggca cgagctgcgg ccatggctgg cttcagcccg gcagcgccga
      421 cgatggcagc catcaccgca tccacctcgg gcgcagaaga aacctgctcc agcgccacgg
      481 cgccgcacaa cacttcggtt tccgatgctg cctgcctgag ttgctgcctg agcgtttctg
      541 cagcgccggt atccagcacc acggcgtatc tgggcttgaa ctgcaggcat tgcttcagca
      601 gtggctcaat gcgcgagttg gcagtcaggg cataggcatg gaaacggtcg gggtggcgcg
      661 cgatcacgtc cagcgtgttg acgcctatgg tgccggtgga accgaggacg gtgacattct
      721 gcggacggct catctcggga tcagcacgct gaggtaaaac ggcagataca cgcaaaaggc
      781 caccagtggc aaagtcgaag tcagcccgtc tatgcgatcc agcacgccgc catgccccgg
      841 aaaaatgctg ccgctatcct ttatgccggc ctgccgcttg gccatggatt caaacaggtc
      901 ccccatcacg gaaagcaccg caatggcgat aaaccccacc acgagccaca aactccagct
      961 gcgccagacg caaagtgcga atccaaacag cgtcacgcca gccaaggcgc caaaaaagcc
     1021 ctcccaagtc tttcccggac tgatttgcgg tgccagcttg cgcctgccga agcgcttgcc
     1081 gctgaaataa gcggcgctat cggccaaggc gaccgtcagt accaccagca gtaccgcctg
     1141 ggggctgaca tgccgcaagt tcagcaaagc gatccagggc ggcaacagca acagccatcc
     1201 aatcagtacc ggtgccacaa gacctctcct gatacgccat tggtgcccca gccacaacgg
     1261 catcagcacc acccagaaca gcgtcgccgc caatagcagc agcgcggaaa ttcgcacgtt
     1321 ccacagcatc gtcccgggca tgccgggcaa caacaacaca ctgatcaatg cggttgctgc
     1381 cagaaagacc agtttcccgc ggcgctggta accgaccatc ccaccccact cccaggcgcc
     1441 aaccagaata aacaccagca ggaacagcgc ccagaccagg tcagtcagaa gaaacagcgc
     1501 gccgagaaac acggcgccga gaatcagggc tgtaatcaag cggttcttaa gcatggccgt
     1561 caggaacaag ttgctcgctg gtacgtccga accgtcgttc gcgctgtcga taggactgga
     1621 tcgcctgctt gaatgccgca tcgtcaaaat ccggccacaa ggcatcggtg tagtaaagct
     1681 cggaatacgc caactgccac agcaggaagt tgctgatgcg ctgctcgccg ccggtgcgga
     1741 tgaacaggtc aggttcaggg gcatagtgca tggatagaaa atcggccaga tcctgctcgg
     1801 taaagtcttg tgccaactcc ggcctggctt gcagcatgcg tttcatcgcc tgcatgatat
     1861 cccagcgacc gccgtaattg gcagcaatgg tcaaggtcaa gcgggtattg tgctgggtca
     1921 gcttttcggc atcatggatc gccttgacca gcaaggggtc gaagcgggag cggtcgccga
     1981 tcatcaccag gcggatctgg ttctcgtgca gcctcgtcac ttcgcgcttc aacgcctcca
     2041 tgaacaggcc catgagaaat ttgacctcgt ccggcggacg ccgccagttt tcgctgctga
     2101 aggcgaacag ggtgagatac tgcacgccca tgtccacgca ccgcctgacc acgtcgcgca
     2161 ctgtctcgac accacgcttg tgacccatga cgcgaggcaa gaagcgcttc ttggcccaac
     2221 gcccgttgcc atccatgatg atggcgatgt gccgcggcac atcaagggat tggggaatgt
     2281 catgcgtgga actggcgaag cgtgccaacg tcagcctttc aacagaaagt gtttaccacg
     2341 gaagcgggat gataaagatg catcatccca actggcttaa atcgccatca ggtcggcttc
     2401 tttagcctgt agcgccttgt ccacttcggc gacgaacttg tcggtcagtt tctggatttc
     2461 gtcctgggca cggcgctctt catcctcgcc aatttccttg tccttgagca gcttcttcaa
     2521 ctggtcattg gcgtcacggc gaatgttgcg caccgacact ttggcgttct cgccttcggc
     2581 acgtaccacc ttggtcagat cgcggcgtcg ctcctcagtc agcggcggca tcggcacgcg
     2641 gatcatgtcg ccgctattgg ccgggttgag cccgaggtcg gaatcgcgga tggccttttc
     2701 gaccttggcc atcatgggtt tttcccatac ctgtacggta atggtacgcg cgtctgccaa
     2761 gccaacgttg ccaacctggc tgatcggcac catattgccg taatactcga ccatcacctg
     2821 gtccagcaga ccggcgtggg cacggccggt acgcaccttg cccagatcgg ccttcagcac
     2881 ctccagcgct ttctgcattt tctgttcggc agttttcttg acgtcgttga tcatgtccgg
     2941 tctcctttaa ggcagcacgc gcgtgccttc gtcctcgccc attaccacgc gtttcaacgc
     3001 gccctgcttg aagatgctga acacacagat cggcagcttc tggtcgcggc acagcgtcag
     3061 tgcagtggcg tccatcacct tgaggttctt gacgatagct tcgtcaaagc tgatgtcctt
     3121 gtagcgcatc gcgtctgggt gcgtcttcgg atcgtcggta tagacgccat ccaccttggt
     3181 ggccttgagt acgatgtcgg cgttcatttc catgccacgc agcgctgcag cggtgtcggt
     3241 agtaaagaac gggttgccgg taccggcgcc gaagatcacc acacgcccct cttccaggta
     3301 acgcatagcc ttgccacgga tatagggctc ggccacttgc tcgatggtca gcgccgattg
     3361 cacgcgactc accagcccaa cctggcgcat cgcgtcctgt agtgccaacg cattcatcac
     3421 cgtggccatc atacccatgt aatcggcagt ggcgcgatcc ataccggctg ctgccggcgc
     3481 cacgccacga aagatgttgc ccccgccgat caccaccgcc acctcgaccc cgagatcgac
     3541 cacctccttg atctccttga cgatgcgctc gatggtggcg cggttgatgc cgtagctgtc
     3601 ctcgcccatc agggcttcgc cggacagctt gagcagtatg cgtttaaagg cgggagcggt
     3661 catgcgctga ttacaccttg gctgcagcag ccacttcggc agcgaagtcg gtcaccttct
     3721 tctcgatgcc ctcacccact acgtacatat tgaaactggc tacgctggca cctctggact
     3781 taagcagttg ctcgatagtc tgcttgtcat ccttgacgaa aggctggctg agcagggtta
     3841 cttccttaag gaatttctgc accgtgccgt cggcgatctt gtccagcatg gcttccggct
     3901 tgccagcctc cctggccttc tcgatcgcca cgcggcgttc ggcttcgatc agttcgggag
     3961 caacaccgga ggaatccagc gacttcggct tggtcgccgc aatatgcatg gcaatatcct
     4021 tggccagtgc ttcgtctccg cccaccacat ccaccaacac accgatcttg ccaccatgta
     4081 cgtagctggc tagtctgccc ttggcttcaa ccgccagaaa acgacgtggg gtaatgtttt
     4141 caccaatctt gccgaccagt tgcgtacgtg catcttcaac cgtaccgttg cccagcttca
     4201 ggccgccgac tgcagcgaca tctgccggtt tgccgttggc gacaaccacg gccagatccc
     4261 gcacgaaagc caggaagtca tcattcttgg cgcagaagtc ggtttcagag ttgacttcaa
     4321 tcatcgcgcc ggttttacca tcggcgctga tgctgatgcc cacggagcct tccgcggcca
     4381 cgcggccggc agccttgctg gccttgttgc cgaagcgcac acgcaggatt tcttccgccc
     4441 gcgccatgtc gccgtcagct tccgacagcg ccttcttgca atccatcatc ggtgcatcgg
     4501 tgcgctcacg cagctccttc accatgcctg cggtaatttc cgccatgttc cactccttga
     4561 atatcacttg ccacgcgacc
//
LOCUS       DAOMFA010000127         4560 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_2553_length_4560_cov_4.047725, whole genome shotgun sequence.
ACCESSION   DAOMFA010000127 DAOMFA010000000
VERSION     DAOMFA010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 4560)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4560)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4560
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2553_length_4560_cov_4.047725"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            116..856
                     /locus_tag="PKW44_06820"
     CDS             116..856
                     /locus_tag="PKW44_06820"
                     /inference="COORDINATES: protein motif:HMM:NF025024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HQR51332.1"
                     /translation="MSISTTTLDRIIDDLARQGWCAIPDFVSTQTVAQLAEETQQLRQ
                     QQVMRPAGVGKGAERTVNAGIRGDFILWLDEPDLTAAQRDYLAGLEQLRLAANASLQL
                     GLFEFEGHLATYPAGSFYRKHIDRFHNDSLRTLTCILYLNQSWQASDGGQLRVYTNGH
                     GEYAIPDDESRRARLPHPYPLPLPGEGDAVSLHEFQVYEHDHFDVLPQGGTLMTFLSD
                     RFWHEVLPASRERMSVTGWFRTRGEVAL"
     gene            853..2283
                     /locus_tag="PKW44_06825"
     CDS             853..2283
                     /locus_tag="PKW44_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HQR51333.1"
                     /translation="MSRVEEIFDPTRFRHYGHELIDLLADYLAASLARQGKVLDWKAP
                     ALEDAQWQAPLPRGATLSPDQLLEKLRTDILPAGLAIHHPRNLGHQVATPLPMAALCD
                     LVAALTNQAMAVYEAGPAATLIERQVIRWLAELIGRDHAEGVLTSGGAQANLTALLAA
                     RQQTAGWDIWKEGAAAGPPLCILASEHAHYSVSRAAGIMGLGVDAVVKVAADPQGRME
                     PAALAASHRQAIASGARVMAVVATAGCTPTGSVDPLAAIGTYCREHGLWLHVDGAHGA
                     SALLSTRHRDAMRGIGLADSVVWDGHKLLYMPAPVSAVLFREPKTSYAAFAQEASYLF
                     QGQSNEEEAYNVGYRTLECTKRMMGLKLWAAFSLYGTEGLGELVDKVFASARLLADKL
                     QAAPDFELLMPPQTNIVCFRYRNGDQAAIRKALVESGAFHLTQVNLHGEVWLRTTVMN
                     PLTAESDLDALLETIRHDAQLSSEAC"
     gene            complement(2377..3852)
                     /locus_tag="PKW44_06830"
     CDS             complement(2377..3852)
                     /locus_tag="PKW44_06830"
                     /inference="COORDINATES: protein motif:HMM:NF018561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1254 domain-containing protein"
                     /protein_id="HQR51334.1"
                     /translation="MELRKLASFTMAAIFLTLAASCGRQEEAKVENSAAEAKATIVAD
                     TKEVEALALATEAYVYAYPLVTMEMTRRVMTNVASAEGTRAPMGQFVRMREYPNAKFR
                     DVTAPNADTLYTTAWLDVSKEPWILSIPDMKDRYFLFPMLDGWTDVFQVPGKRTTGTK
                     AQKYAITGPGWTGTLPEGVTEYKSPTGIVWILGRIYCTGTPQDYKEVHALQDQVSVVP
                     LSSYGKSYIPAAGSVDPAIDMKTAVREQVNALDGAAYFKLFADLLKTNPPAAEDAPMV
                     AKLAKLGIVPGQDFDAAKLEPAVAKGIDAAAKPAQEIIMGWMKAGIAAGDMKLENGWL
                     FTTKTGLYGTGYTQRALITAIGLGANRPQDAVYPTSEGPDIVKKYSGEKKYVMHFEKG
                     HLPPVNGFWSLTMYDAQYFFVDNPLNRYSISERNKLKANADGSVDLYIQNESPGKDKE
                     SNWLPAPKEQFILMLRMYWPKDEPPSIIDGTWKIPEVKEAS"
     gene            complement(4104..>4560)
                     /locus_tag="PKW44_06835"
     CDS             complement(4104..>4560)
                     /locus_tag="PKW44_06835"
                     /inference="COORDINATES: protein motif:HMM:NF014037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Mut7-C RNAse domain-containing protein"
                     /protein_id="HQR51335.1"
                     /translation="RKLVKLLCDEMLKGLARWLRTAGYDVAMEPDSTPNRALIEGALS
                     EHRLLLTRDRTLLEIRNAKEVVLLLESNDLEKCAREATRKLGIDWLLNPFSRCSLCNT
                     ILIETERPADFPSDIEQAFFCGTCNKYYWHGGHVERMRCKLERWQLAFR"
BASE COUNT          907 a         1402 c         1307 g          944 t
ORIGIN      
        1 aacgaccccc accatgtcaa ggtggtgctc taaccagctg agctacgcgc ctgaagagct
       61 gcgcattcta cagaaaaggc caccgcagcg caacgcgcta taattgcgcc gaaccatgtc
      121 gatctctacc accaccctcg accgcatcat tgacgacctc gcccggcaag gctggtgtgc
      181 aatacctgat ttcgtttcaa cgcagacggt cgcccaactg gctgaggaaa cgcagcagct
      241 ccgccagcag caagtcatgc ggcccgccgg ggtcggaaag ggtgcggagc gtaccgtcaa
      301 cgccggcatc cgcggcgatt tcatcctgtg gctggatgag cccgatctca ccgccgccca
      361 gcgcgattac ctggccggcc tggaacaatt gcgtctggcc gccaacgctt ctctgcaact
      421 cggcctgttc gagttcgaag gccacctggc cacctatccg gccggcagct tctaccgcaa
      481 gcacatcgac cgcttccaca acgacagcct gcgcaccctg acctgcatcc tctacctcaa
      541 ccagagctgg caggcttccg acggcggcca gctacgggtt tacaccaacg ggcatggcga
      601 atatgccatt cctgatgatg aatctcgccg tgcccgtctc cctcacccct accctctccc
      661 gcttccgggc gagggagacg cggtctcgct gcacgagttt caagtttacg agcatgacca
      721 tttcgacgtg ctgccgcagg gcggcaccct gatgaccttc ctttccgatc gcttctggca
      781 cgaagtgctg ccggccagcc gcgagcgcat gagcgttacc ggctggttca ggacacgtgg
      841 cgaggtggca ctgtgagccg ggtcgaagag atattcgacc cgacccgctt tcgccactac
      901 ggccatgaac tgatcgactt gctggcggat tacctggccg ccagcctggc cagacagggc
      961 aaggtgctgg actggaaagc gccggcgctg gaggatgccc agtggcaggc gccgctgcct
     1021 cgaggtgcca cgctctcgcc cgatcaattg ctggaaaagc tgcgtaccga catcctgccg
     1081 gcaggcctcg ccatccacca cccacgcaac cttggtcacc aggtagcgac gcccctgccg
     1141 atggccgccc tgtgcgactt ggtcgcggcg ctgaccaacc aggccatggc agtatacgaa
     1201 gccggcccgg ccgccaccct gatcgagcgc caggtcatcc gctggctggc cgaactgatc
     1261 ggccgggatc atgcagaggg cgtgctcacc tcgggcggcg cgcaagccaa cctcaccgcg
     1321 ttgctggccg cacgacagca gacggcggga tgggacatct ggaaagaagg cgctgccgcc
     1381 ggcccgccgc tgtgcattct cgccagcgaa cacgcacatt attcagtgag tcgcgctgcc
     1441 ggcatcatgg ggctgggcgt ggacgccgtg gtcaaggtcg cggcggatcc ccagggacgc
     1501 atggagccag cggcgctggc ggcatcccac cggcaagcga tagccagcgg cgcccgggtc
     1561 atggccgtgg tcgccaccgc cggctgcacc cccaccggca gcgtcgaccc gctggccgcg
     1621 atcggcacgt attgtcggga acacggcttg tggctgcacg tggacggcgc ccacggcgcc
     1681 tccgccctgc tctccactcg gcaccgcgat gccatgcgcg gcatcggact ggccgattcg
     1741 gtagtgtggg acggtcataa attgctctac atgcccgcac cggtttccgc tgtgctgttc
     1801 agggaaccca aaacgagtta cgccgccttc gcgcaggagg cttcctacct gttccagggc
     1861 cagtcgaacg aggaagaagc ctacaacgtc ggctaccgca cgctggagtg caccaagcgc
     1921 atgatgggcc tcaagctgtg ggcagcattt tcgctatatg gcacggaggg cttgggggaa
     1981 ctggtagaca aggtattcgc cagcgcacgg ctgctggccg acaagctgca agccgcgccg
     2041 gatttcgaac tgctgatgcc gccgcagacc aatatcgtct gcttccgtta tcgcaacggc
     2101 gaccaggcgg cgatcaggaa ggcattagtg gaaagcggcg ccttccatct gacgcaggtc
     2161 aatctgcatg gtgaagtctg gctgcgcact acggtgatga acccgctcac agcggagagc
     2221 gacctcgatg cgctactgga aaccattcgc catgatgccc aattgtcatc ggaagcctgc
     2281 taacgacctt ggattcaagc gtgagtactg tggccatcgt cgttgagcgt acttaaaaag
     2341 cacccccgag tgctgcttcg ggggtgctgc tggaagttaa gatgcctctt ttacttcagg
     2401 gatcttccaa gtaccgtcaa tgatcgaagg aggctcatct ttcggccagt acatcctcag
     2461 cataagaata aactgctcct ttggtgccgg cagccaattg gattctttgt ctttaccggg
     2521 cgattcgttc tggatataca aatcgacaga tccatcagcg tttgccttga gcttgttgcg
     2581 ctcgctgatg ctgtaacggt tgagggggtt gtcgacaaag aaatactggg catcgtacat
     2641 ggtcaacgac cagaacccgt tgaccggagg caaatgaccc ttttcgaagt gcatgacgta
     2701 cttcttctcg ccggagtact tcttgacaat gtccggcccc tccgaagtcg ggtacaccgc
     2761 atcctgcgga cgattggcgc caaggccgat cgcggtgata agcgcacgtt gcgtgtagcc
     2821 ggtaccatac agacccgtct tggtggtaaa taaccagccg ttctcaagct tcatgtcacc
     2881 tgcggcgata ccggccttca tccagcccat aatgatttcc tgtgccggct tggcagcagc
     2941 gtcgatgccc ttggcaactg cgggttcgag cttggcggcg tcgaaatcct gtccgggaac
     3001 gatgccgagc ttggcgagtt tggccaccat gggcgcatcc tcggccgcag gcgggttggt
     3061 cttcagcaga tccgcaaaca gcttgaagta agccgcgccg tccaatgcgt tgacctgttc
     3121 cctgacagca gtcttcatgt cgatcgcggg gtcaactgag ccagctgccg ggatataaga
     3181 tttgccgtag gaagagaggg gaaccaccga gacctgatcc tgcaaagcat gcacctcttt
     3241 gtaatcctgt ggcgtgccgg tgcagtagat gcgaccgagt atccagacga taccggtggg
     3301 cgatttgtat tcggtgacgc cttccggtag cgtgcccgtc cagcccggcc cggtgatggc
     3361 gtacttttgc gccttggttc cagtcgtgcg ttttcctgga acctgaaaga catcggtcca
     3421 gccatccagc atggggaaca ggaagtagcg gtctttcata tcaggaatgc tgagtatcca
     3481 gggttccttc gacacgtcga gccatgctgt cgtatagagc gtgtccgcat tcggcgcggt
     3541 cacgtccctg aacttggcgt tgggatattc ccgcatccgc acaaactgcc ccatcggagc
     3601 acgggtacct tcggccgagg caacattagt cataacccgc cgcgtcattt ccatggtgac
     3661 aagggggtag gcatagacat aggcttcggt ggccaacgcc aatgcctcga cttctttggt
     3721 atcagccaca attgtggcct tcgcctcggc ggcagaattt tctaccttgg cttcttcctg
     3781 cctgccacag gatgcggcca gggtgaggaa gattgctgcc attgtgaagc tggcaagttt
     3841 tcgcaattcc atcatgttta ttcccttctg tattgttcat gagggtgaga aattctttgt
     3901 gttttttcaa aacgccctgc cagtttattt cttgaacgaa atttctgaaa gaaagcaaaa
     3961 catctacatt gccctgagca gtactttaaa tatagtccag tcattctaat ttatctacag
     4021 gccaatatac gagtcatcgc ggacgtatgg gtgccagcaa ctaatgtccg cctctggccg
     4081 atagtcgcct tcggcagcca tgttcaccga aacgccaact gccaccgctc cagcttgcag
     4141 cgcatacgct ctacatgccc accatgccag taatacttgt tgcaggttcc gcaaaagaaa
     4201 gcctgctcga tgtccgacgg gaaatctgcc gggcgctcag tttcaatgag tattgtgttg
     4261 cacagcgagc agcggctgaa aggattcaac agccagtcga tgccgagttt ccgggtggct
     4321 tcccgcgcgc atttttccag atcgttgctc tccagcagaa gcacaacttc ttttgcgttg
     4381 cgaatttcca gcagggtacg gtcgcgcgtc aacagcagac ggtgttctga caatgcgcct
     4441 tcaatcagcg cgcgattcgg cgtggagtcg ggttccatcg cgacatcgta gcccgcggtg
     4501 cgcagccagc gcgccaaccc cttgagcatt tcgtcgcaga ggagtttcac cagcttccgg
//
LOCUS       DAOMFA010000128         4542 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_2568_length_4542_cov_5.227992, whole genome shotgun sequence.
ACCESSION   DAOMFA010000128 DAOMFA010000000
VERSION     DAOMFA010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 4542)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4542)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4542
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2568_length_4542_cov_5.227992"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            117..2105
                     /gene="tkt"
                     /locus_tag="PKW44_06840"
     CDS             117..2105
                     /gene="tkt"
                     /locus_tag="PKW44_06840"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019897311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HQR51336.1"
                     /translation="MATRKELANAIRALAMDAVQKANSGHPGAPMGMADIAEVLWNNH
                     LRHNPANPKWADRDRFILSNGHGSMLIYSLLHLSGYDLPMDEIKTFRQLHSKCAGHPE
                     YGYAPGVETTTGPLGQGITNAVGFAMAEKLMANQFNKPGHEIVDHRTWVFLGDGCMME
                     GISHEACALAGTWGLGKLTAFWDDNGISIDGHIEGWYTDDTAKRFEAYGWHVVPNVDG
                     HDPAAINAAIEEAKKVTDKPTLICTKTIIGWGSPNKSGSHDCHGSALGDAEVAATRAN
                     IGWAHEPFVIPKDVYEGWDARTKGQGHETLWNNKFAEYAKAYPAEAAEFKRRMAGELP
                     ANWKEVTSAFIKATNEKAEAIATRKASQNAINALAAVLPEFVGGSADLTGSNLTNWTG
                     AHHVHGKKPGNYISYGVREFGMAHIMNGMALHGGILPFGGTFHMFSDYMKSGIRMSAL
                     MHQRVIYVLSHDSIGQGEDGPTHQPVENTAGLRYIPRLDVWRPADSTETAVAWVAAVE
                     RSEGPAALVLSRQNVPGINHDNAQLDLIRKGGYVFSDAAGKADVILIANGSELDLAIK
                     AAAELTAAGKKVRVVSMPSTNVFDRQDQAYKDSVLTPGVKRVAVEAAHPDFWRKYVGL
                     DGAVVGINTFGESAPGGVLLKHFGFTVENVVNTAKSVM"
     gene            2184..3182
                     /gene="gap"
                     /locus_tag="PKW44_06845"
     CDS             2184..3182
                     /gene="gap"
                     /locus_tag="PKW44_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HQR51337.1"
                     /translation="MAIKVGINGFGRIGRMVFRAAVQDFPDIEVVAINDLLDPDYLAY
                     MLKHDSVHGRFKGDVSVSGNNLVVNGKTIRLTAEKDPANLKWNEVGADIIVECTGFFL
                     TKETCQKHIDAGAKKVVQSAPSKDDTPMYVYGVNHDTYNGEAIVSAASCTTNGLAPVA
                     KVLNDNFGIKRGLMTTVHAATATQKTVDGPSNKDWRGGRGILENIIPSSTGAAKAVGK
                     VIPSLNKKLTGMAFRIPTSDVSVVDLTCELVKEASYEDICKAMKAASEGALKGGVLGY
                     TEDKVVSTDFRGETCPSVFDAGAGIALDPTFVKVVAWYDNEYGYTCNMMRLVRHVA"
     gene            3275..4480
                     /locus_tag="PKW44_06850"
     CDS             3275..4480
                     /locus_tag="PKW44_06850"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HQR51338.1"
                     /translation="MSVIKMNDLDLKGKRLLIREDLNVPQNEDGTVADDTRIRASLPT
                     IIHAMNAGAKVMLMSHLGRPKEGPTEGRYSDVDSLRPVAEHLGKLLGKEVRLYQDWLG
                     NGRLYVQQMRDSEVAVLENVRFNIGENNNEDRLSWEMAQLCDIYVMDAFGTAHRAQAS
                     THGVAKFAPVACAGPLLAAELDALGKALKEPKRPLVAIVGGSKVSTKLTVLDSLSNIV
                     DQLIVGGGIANTFIKAAGFNVGKSLYEADLVEEAKRLMEAAKAKGSEIPVPVDVVVGK
                     KFAATEPAVVKKVSEVVDDDMIFDIGPETSAIYAEYIKKAGTIVWNGPVGVFEFDQFG
                     EGTRALGMAIAVSPAFSIAGGGDTLSAIAKYDIADRVSYISTGGGAFLEFLEGKKLPA
                     VAALAARAG"
BASE COUNT          956 a         1374 c         1381 g          831 t
ORIGIN      
        1 ccataaaaat ataattgcct gactccatgg ggaattccga aatgattttg aggtgcggct
       61 tctgcccgaa ggggtgggag tctttttctc tttgaagcta aacgatagga atgattatgg
      121 cgactcgtaa agaactggca aacgccatcc gcgcactggc gatggatgct gtccagaagg
      181 caaactcggg acacccgggc gccccgatgg gtatggcgga tatcgccgaa gtgctgtgga
      241 acaaccattt gcggcacaac ccggccaatc cgaagtgggc cgaccgcgac cgcttcattc
      301 tgtccaacgg ccacggctcc atgctgatct actcgttgct acacctgtcc ggctacgatc
      361 tgccgatgga cgaaatcaag acattccgcc aactgcactc caagtgtgcc ggccacccgg
      421 aatacggtta cgcccctggc gtggaaacca ccaccggccc gctgggtcag ggcattacca
      481 acgccgtcgg cttcgccatg gcagaaaagc tgatggccaa ccagttcaac aagcccggcc
      541 atgaaatcgt cgaccaccgc acttgggtgt tcctgggtga cggctgcatg atggaaggca
      601 tctcccacga agcctgcgcg ctggccggca cctggggcct gggcaagctg accgcattct
      661 gggatgacaa cggcatctcc atcgacggcc acatcgaagg ctggtacacc gatgacaccg
      721 ccaagcgctt cgaagcctac ggctggcacg tggtgccgaa cgtcgatggt catgatccag
      781 cagccattaa tgccgcgatc gaagaagcca agaaggttac cgacaagccg accttgattt
      841 gcaccaagac tatcatcggc tggggttccc ccaacaagtc cggcagccac gactgccacg
      901 gttccgcgct gggcgacgcc gaagtggcag ctacccgtgc caacatcggc tgggcccacg
      961 agccgttcgt gatccccaag gacgtctacg aaggttggga tgcccgcacc aagggtcaag
     1021 gtcacgaaac cctgtggaac aacaagttcg ccgaatacgc caaggcctac ccggcagaag
     1081 ccgccgagtt caagcgccgc atggcaggcg aactgccggc caactggaag gaagtgacct
     1141 ctgctttcat caaggccacc aacgagaagg ctgaagccat tgccacccgc aaggcttcgc
     1201 agaatgccat caacgcgctg gctgccgtgc tgccggaatt cgttggcggt tccgccgacc
     1261 tgaccggttc caacctgacc aactggacgg gtgcacacca cgtccatggc aagaagccgg
     1321 gcaactacat ctcctacggc gtacgcgagt ttggcatggc acacatcatg aacggcatgg
     1381 cgttgcatgg cggcatcctg ccgttcggcg gcaccttcca catgttctcc gactacatga
     1441 agtccggtat ccgtatgtcc gcgttgatgc accaacgcgt gatctatgtg ctgagccatg
     1501 actcgatcgg ccagggcgaa gacggcccga cccaccagcc ggtggaaaac actgccggcc
     1561 tgcgctacat cccgcgtctg gatgtatggc gtccggccga ttccaccgaa acggcagtgg
     1621 cctgggtggc cgcggtcgag cgtagcgaag gcccggctgc gctggtgctg tcgcgtcaga
     1681 acgtgcctgg catcaaccac gataatgcgc agcttgacct catccgcaag ggcggctacg
     1741 tattctccga cgctgcaggc aaggccgacg tgattctgat cgccaacggc tccgaactgg
     1801 acctggcgat caaggccgct gccgaactca ctgctgccgg caagaaggtg cgtgtggttt
     1861 ccatgccgtc taccaacgtg ttcgatcgtc aggaccaggc ctacaaggac agcgtgctga
     1921 ccccgggcgt gaagcgtgtg gccgtggaag cggcccaccc cgacttctgg cgcaagtatg
     1981 ttggcctgga cggcgctgta gtcgggatca acactttcgg tgaatccgct cccggtggcg
     2041 tgctgctcaa gcatttcggc tttaccgtcg agaatgtggt gaacaccgcg aagtctgtga
     2101 tgtaaccgta tgcagtaatt cttgaagagg gagcctgcaa attttttcgg gctccctttt
     2161 ttgtttctga gaggggtgta gcaatggcaa tcaaagttgg tatcaatggt ttcggccgta
     2221 tcggccggat ggtattccgc gccgccgtcc aggattttcc ggatatcgag gtggtggcga
     2281 tcaatgacct gctggatccg gattatctgg catacatgct caagcacgac tcggtgcatg
     2341 gccgcttcaa gggcgatgta tcggtcagcg gcaacaacct ggtggtcaac ggcaagacca
     2401 tccgcctgac cgccgaaaag gatcccgcca acctgaagtg gaacgaggtt ggcgcggata
     2461 tcattgtcga atgcaccggt ttcttcctga ccaaggaaac ctgccagaag cacatcgatg
     2521 ccggcgccaa gaaggtggtg caatccgccc cgtccaagga cgacacgccg atgtacgtct
     2581 acggcgtcaa ccatgacacc tacaacggcg aagccatcgt ttccgctgca tcctgcacca
     2641 ccaacgggct ggccccggtg gccaaggtgc tgaacgacaa cttcggcatc aagcgcggcc
     2701 tgatgaccac cgtgcatgcc gccaccgcca cacaaaagac cgtcgatggc ccctctaaca
     2761 aggattggcg cggcggccgc ggtattctgg aaaacatcat tccttcctcc accggcgccg
     2821 ccaaggcggt gggcaaggtg attccttcgc tgaacaagaa gctgaccggt atggcgttcc
     2881 gcatcccgac ttccgacgtg tcggtggttg acctgacctg cgaactggtt aaggaagcca
     2941 gctacgagga catctgcaag gcaatgaaag cggcgtccga aggcgcgctg aagggggggg
     3001 ttctgggcta caccgaagac aaggtggttt ccaccgactt ccgcggcgaa acctgcccgt
     3061 cggtgttcga cgccggtgcc ggcatcgcgc tggatccgac tttcgtcaaa gtggtggcct
     3121 ggtacgacaa cgagtatggc tacacctgca acatgatgcg tctggtacgc cacgtcgctt
     3181 gaaaatagcg caggcgagat cgcctgtatg gccgcgacag cggcctcata actaaatgca
     3241 ggcgacctcg cctgcgctgc atggggagct gaaaatgtcc gtaatcaaga tgaacgacct
     3301 ggatctgaag ggcaagcgtc tgctgatccg ggaggatctg aatgtgccac agaatgagga
     3361 cggcacggtg gctgatgaca cccgtatccg cgcttcgctg cccaccatca tccacgccat
     3421 gaacgccggc gccaaggtga tgctgatgtc gcatctgggg cgcccgaagg aaggcccgac
     3481 cgaaggccgc tattccgatg tcgacagcct gcggccggtg gccgagcatc ttggcaagct
     3541 gctcggtaag gaggtccggc tgtatcagga ttggctgggc aacggcaggc tctacgtgca
     3601 gcagatgcgc gacagcgagg tggcggtcct cgaaaatgtg cgcttcaata tcggcgagaa
     3661 caacaatgaa gaccgtctgt cctgggaaat ggcgcagttg tgcgacatct acgtgatgga
     3721 tgcgttcggg accgcccatc gcgcccaggc ctccacccac ggcgtggcga aatttgctcc
     3781 ggtcgcctgc gccggcccgc tgctggcggc tgaactggat gcgcttggca aggcgctcaa
     3841 ggagccgaag cgcccgctgg tggcgattgt cggcggcagc aaggtatcca ccaagctcac
     3901 cgtactcgat tccctgtcca atatcgtgga ccagctcatc gtcggcggcg gcatcgccaa
     3961 caccttcatc aaggccgccg ggttcaatgt cggcaagtcg ctttacgaag ccgacctggt
     4021 ggaggaagcc aaacggctga tggaagccgc caaggccaag ggcagcgaga ttccggtgcc
     4081 ggtggatgtg gtggtcggca agaaattcgc cgcgaccgag ccggcagtgg tcaagaaggt
     4141 gagcgaagtg gtcgatgacg acatgatctt cgatatcgga cccgagacct cggcaatcta
     4201 cgccgagtac atcaagaagg ccggcaccat cgtctggaac ggcccggtcg gcgtgttcga
     4261 attcgaccag ttcggcgagg gcaccagggc actgggcatg gcgattgccg taagcccggc
     4321 gttctccatc gctggcgggg gcgatacgct gtcggcgatc gccaagtacg acatcgccga
     4381 ccgcgtatcc tacatttcca ccggcggcgg cgccttcctc gagttcctcg agggcaagaa
     4441 actgccggcc gtcgcggccc tggcggcgcg cgccggctga tggcggtcct tttcttcgac
     4501 ctggacggca cgctgatcga ttcggagatc ggcatcaccc gc
//
LOCUS       DAOMFA010000129         4527 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_2578_length_4527_cov_4.726297, whole genome shotgun sequence.
ACCESSION   DAOMFA010000129 DAOMFA010000000
VERSION     DAOMFA010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 4527)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4527)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4527
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2578_length_4527_cov_4.726297"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..534
                     /locus_tag="PKW44_06855"
     CDS             <1..534
                     /locus_tag="PKW44_06855"
                     /inference="COORDINATES: protein motif:HMM:NF014640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HQR51339.1"
                     /translation="DFVADGRAPTPTAAAELVTPDGTALLRQIAQTATTLERQMVRNL
                     AERAQKLDLLGYRLIHPGEKIHRHQEQLLHLRTRITATLGRRLENIGNQFSSLSNALQ
                     RGMAQQLSLHRTQLLRVAQNLEHLSPQQVLNRGYSLVRTAEGVVVRSSVELKEDEAVH
                     LTFAQGGAEATITRTTP"
     gene            555..836
                     /locus_tag="PKW44_06860"
     CDS             555..836
                     /locus_tag="PKW44_06860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR51340.1"
                     /translation="MSRHILPTASTMVGVCMTVIGLIKLMPGGGGTTRIDEILALDSL
                     IFVTSAFFSYLSIRSSLKEATYERWADNTFMAALAVMALTTCVFALELL"
     gene            953..2833
                     /gene="kup"
                     /locus_tag="PKW44_06865"
     CDS             953..2833
                     /gene="kup"
                     /locus_tag="PKW44_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium uptake protein"
                     /protein_id="HQR51341.1"
                     /translation="MQTPDSNKSRLAALSLAALGVVFGDIGTSPLYTMKEVFASHHPM
                     PVTPDNVLGVLSLILWALIMVVTVKYVAFVMRADNHGEGGIMALIALASQQAGKNKRR
                     QRFIMLAGIVGACMFFADGMITPAISVLSAVEGLEIASPHLHKMIIPVTLGVLFVLFW
                     MQRHGTATVGTLFGPIMLLWFGTLATLGIASILQNPEVLRALNPLHAVHFFTGNHWLA
                     FVALGAVVLAVTGGEALYADMGHFGRRPIRLAWFGFVLPALILNYFGQGALILNNPEA
                     IKNPFYLLAPEWLLYPLVGLATVSTVIASQAVISGTFSVSRQALQLGYMPRVQVQHTS
                     ESEAGQIYLPGVNWWLMVAVMALVLGFGSSSNLAAAYGIAVTGDMVITTVLAALVFHG
                     VWGWSWARTLALAAMFLTVDLAFFGANILKIPAGGWFPILVGVVIFTLMLTWKRGREL
                     LLKRLREEAMELGPFIEAISGHPPARVHGTAIFMTPNPNGVPHAMLHNLKHNKVLHEQ
                     VVLVTVRVSDVPHVDSAERVAVNQLGNGFYRVIVNYGFKDEPDLPRDLDLCAVHGLKL
                     DMMDSSFFIGKETLIPRRHSEMAFWRERLFITMFRNADSVTNYFKLPPNRVVELGAQV
                     AL"
     gene            2903..3349
                     /locus_tag="PKW44_06870"
     CDS             2903..3349
                     /locus_tag="PKW44_06870"
                     /inference="COORDINATES: protein motif:HMM:NF024890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-hand domain-containing protein"
                     /protein_id="HQR51342.1"
                     /translation="MNKLTSLLLASTLAFALPALADHDGMGEHCDMHKKQHSPLAEAD
                     TNKDGSIDKAEAQAMHDKHFDKMDANHDGKLSKEEVAACKNDGMHDKGSMGFKKADKD
                     NDGTLDKKEAKSLPNVSKHFDEIDVDKDGTVSRDEVHNFMKDQHPH"
     gene            3376..4212
                     /locus_tag="PKW44_06875"
     CDS             3376..4212
                     /locus_tag="PKW44_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020182397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HQR51343.1"
                     /translation="MKKFLLPLFAAIALLAGCATTQTTQSGAVGVERKQFMMLSEKQV
                     DQMAAQSYLQTLKEAETKKTLNSNPQQTERVRAIANRLIAQTGVFRPDAANWKWEVNV
                     QESKDVNAYCMPGGKIMVYTGLIDKLNPTDDELANVMGHEISHALREHGRERMSRAYA
                     QQIGLVGLAVALGATSKDRNAAANTMMIGSTVTALALTLPNSREAEREADRMGLELSA
                     RAGYDPNAAITLWQKMGAQGGSKPPEWLSTHPSEESRMKELQSLIPTVMPLYQEARAK
                     KT"
     gene            4288..>4527
                     /locus_tag="PKW44_06880"
     CDS             4288..>4527
                     /locus_tag="PKW44_06880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR51344.1"
                     /translation="MNERIGEIINRIKALESELADELDRARERINYQLLDRKVRFARE
                     VLLQHRKLKIGLLRYALFPRLRHILTAPFVYAVFPP"
BASE COUNT          991 a         1358 c         1291 g          887 t
ORIGIN      
        1 gatttcgtcg ccgacgggcg agcgccaaca ccgaccgccg cagcggaact ggtcacaccc
       61 gacggtacag cgctgctgcg gcaaatcgcg caaacggcca caacgctgga gcggcaaatg
      121 gtacgcaact tggccgagcg tgcgcaaaag ctggacctgc tgggttaccg cctgattcat
      181 cccggcgaga aaatccaccg ccaccaagag cagttattac atctgcggac caggatcacc
      241 gccaccctgg ggcgccggct tgaaaacatc ggcaaccaat tctcgtcgtt gtcgaacgcg
      301 ctgcagcgtg gaatggcgca gcagctttcc ctgcatcgta cccagttgct gcgggttgcg
      361 caaaacctcg aacacctcag cccacaacaa gttctgaacc gtggatacag cctggtgcgc
      421 acggccgagg gcgtggtcgt gcgcagcagt gtggaactca aggaagacga agcggtccac
      481 cttacctttg cgcaaggtgg cgcggaagcc actatcacac gcaccacacc atgagtgagc
      541 ctataagaaa ccaattgtcc cgacacatcc tgccgacagc ctcgaccatg gtgggcgtct
      601 gcatgaccgt gattggcttg atcaagctga tgccgggcgg cggcgggacg accaggatag
      661 acgaaattct ggcgctggac agcctgatct ttgttaccag cgccttcttt tcctatcttt
      721 ccattcgtag ttcgctaaag gaagcaactt atgaacgctg ggccgacaac accttcatgg
      781 ctgccttggc ggtcatggcg ctgacgacct gtgtttttgc tctggagctg ctgtagaaac
      841 ggctccggga ctgccatatg gagttttgca ctgcatcacc tcattgaaga tagccgcggt
      901 tgacaagtag ggataaaatg ccaccttctt gccatcggtt caggtagcac acttgcaaac
      961 cccagactcg aacaaatcgc gccttgcagc gctctccctg gcggcactcg gcgtcgtgtt
     1021 cggcgacatc ggcaccagcc ccctctacac catgaaggaa gtctttgcca gccatcaccc
     1081 gatgccggtg acgccagata acgtgctggg cgtgttgtcc ctgatcctgt gggcactcat
     1141 catggtagtg acggtaaaat acgtcgcttt cgtaatgcgt gccgacaacc atggcgaggg
     1201 cggtatcatg gcgctgatcg cgctggccag ccagcaggcg gggaagaaca aacgccgcca
     1261 gcgattcatc atgctggccg gtatcgttgg tgcctgcatg ttcttcgcgg atggcatgat
     1321 taccccggct atttcggtgc tgtcggcggt ggaaggtctg gaaatcgcct ccccgcatct
     1381 gcacaaaatg attattcccg tcaccctggg cgtgctgttc gtgctgttct ggatgcagcg
     1441 gcatggcacg gccactgtcg gcaccctgtt cggtcccatc atgctgctgt ggttcggcac
     1501 tctggcaacg ctgggcatcg ccagcattct gcaaaaccca gaagtgctgc gcgcgctcaa
     1561 cccgctgcat gccgttcatt tctttaccgg caaccattgg ctggcattcg tcgcgctggg
     1621 cgcggtggtg ctggcggtca ccggcggcga ggcgctgtat gccgacatgg gacatttcgg
     1681 cagaaggccg atccgcctgg cttggttcgg tttcgtattg ccggcgctga tcctcaacta
     1741 cttcggccag ggcgcgctga tcctgaacaa cccggaagcc atcaagaatc cgttctacct
     1801 gctggctccg gaatggctgt tgtatccgct ggtaggactg gcaacggtat cgacagtcat
     1861 cgcgtcgcag gcggtgatct cgggcacctt ctcggtgtcg cgccaggcgt tgcaactcgg
     1921 ctacatgcca cgggtgcaag tgcagcatac ttcggaaagc gaagccggac agatttatct
     1981 gcccggcgtc aactggtggc tgatggtagc ggtgatggct ctggtgttag gcttcggctc
     2041 ctcgtctaac ttggcggcgg cctacggcat cgccgtcacc ggcgacatgg tcatcaccac
     2101 ggtactggcg gcgctggtgt tccacggtgt ctggggctgg agctgggcgc gcacgctggc
     2161 gctggcggcg atgttcctca ccgtggacct ggccttcttc ggcgccaata tcctgaaaat
     2221 ccccgccggc ggctggtttc ccatcctggt tggcgtagtg atctttaccc tgatgctgac
     2281 ctggaaacgc ggccgcgaac tgctgctcaa acgtttacgg gaagaggcga tggagctggg
     2341 tccgttcatc gaggccatca gtgggcatcc tcccgcacgc gtccatggca cggccatctt
     2401 catgaccccc aaccccaacg gcgtgccgca tgccatgctg cacaacctca agcacaacaa
     2461 ggtactgcac gagcaggtgg tattggtgac agtacgagtc agcgatgtgc cgcatgtcga
     2521 cagcgcggaa agggtcgccg tgaatcaatt gggcaacggc ttctatcgcg tcatcgttaa
     2581 ctatggtttc aaggatgagc ccgacctgcc gcgtgacctg gatctgtgcg cagtgcacgg
     2641 cctgaagctc gacatgatgg acagctcgtt cttcatcggc aaggaaacgc tgatccccag
     2701 aagacattcc gaaatggcgt tctggcgcga gcggctgttc atcaccatgt tccgcaatgc
     2761 cgacagcgtg accaactatt tcaagctgcc ccccaaccgg gtggtggaac tgggcgcgca
     2821 agtcgcgctc taaccagcgg cttgacaacc aaatatccac tgactcgtta agccccttgt
     2881 ttatacctac tggagatcaa ccatgaacaa actcacctcc ctgctacttg catccaccct
     2941 cgcctttgcc ctgccggcgc tagccgatca cgacggtatg ggcgagcact gcgacatgca
     3001 caagaagcag cactcccccc ttgccgaagc tgataccaac aaagatggca gcatcgacaa
     3061 ggctgaagct caggccatgc acgacaaaca tttcgacaag atggacgcca atcacgacgg
     3121 caagctaagc aaggaagaag tagctgcctg taagaatgat ggcatgcatg acaagggcag
     3181 catgggtttc aagaaggccg acaaggacaa tgacggcacc ctggacaaaa aagaggccaa
     3241 gtccctgccc aacgtcagca agcatttcga cgagatcgac gttgacaagg acggcaccgt
     3301 aagtcgcgac gaagtccaca acttcatgaa agatcagcac ccgcactaaa gctggatgat
     3361 ttgtcgctta tgaaaatgaa aaaattcctc cttccccttt tcgcggccat cgccttgctg
     3421 gccggctgcg ccaccacgca gaccacccag tccggcgcgg tcggcgtgga acgcaagcag
     3481 ttcatgatgc tttccgagaa gcaggtagac cagatggcgg cacaaagcta tctgcaaacg
     3541 ctgaaggaag cggaaaccaa gaagacgctt aactccaacc cgcagcagac cgagcgtgtg
     3601 cgcgccatcg ccaatcgcct gattgcccag accggcgtgt tccgcccgga cgccgccaac
     3661 tggaaatggg aagtcaatgt gcaggagagc aaggacgtca atgcttactg tatgccgggc
     3721 gggaaaatca tggtctacac cggcctgatc gacaagctga atcctactga cgacgaactg
     3781 gccaacgtca tgggacatga aatttcgcac gccttgcgcg aacatgggcg cgagcgcatg
     3841 tcgcgcgctt atgcccagca aatcggcttg gtggggttgg ccgttgcgct aggggcgaca
     3901 tcgaaagaca gaaatgccgc tgccaatacc atgatgatcg gctctaccgt caccgcgctg
     3961 gccttgaccc tccccaatag ccgggaggcg gagcgggaag cggatcgcat gggactggag
     4021 ctttccgctc gcgccggtta tgaccccaat gcggccatca cgctctggca gaaaatgggg
     4081 gcgcaggggg gctccaaacc gccagaatgg ctaagcaccc acccgtcaga ggagtcccgc
     4141 atgaaggaac tgcagagcct gattcccacc gtcatgccgc tatatcagga agcaagggcc
     4201 aagaaaacct gatttgatgt cacaatagcc acatcgcaag ggcgccttgg gcgcccttag
     4261 ttttttggaa tctccagaga cagtcatatg aacgaaagaa tcggcgaaat catcaaccgt
     4321 atcaaggcgc ttgagagcga actcgcagac gaactcgaca gggcgcgcga acgcatcaac
     4381 taccagttgc ttgaccggaa agtccgcttt gcacgcgaag tcctgctaca gcaccgcaag
     4441 ctgaaaatcg gtctgcttcg ctatgccctg tttccgcggc tgcgccatat actgacggcc
     4501 cctttcgttt atgcggtgtt tcccccg
//
LOCUS       DAOMFA010000130         4465 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_2631_length_4465_cov_5.356916, whole genome shotgun sequence.
ACCESSION   DAOMFA010000130 DAOMFA010000000
VERSION     DAOMFA010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 4465)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4465)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4465
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2631_length_4465_cov_5.356916"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..378
                     /locus_tag="PKW44_06885"
     CDS             <1..378
                     /locus_tag="PKW44_06885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR51345.1"
                     /translation="KIHGDWAMIGERVNAVLSRIEKMPIGEVIEDARKTLATLNDTLD
                     KFGKLAQRLDSEVAPEAVATLASAKHAADNMEKMLAADSPMQEDMRAALQELTRAAES
                     MRVLTDYLETHPEALIRGKKKEE"
     gene            381..974
                     /locus_tag="PKW44_06890"
     CDS             381..974
                     /locus_tag="PKW44_06890"
                     /inference="COORDINATES: protein motif:HMM:NF015823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqiC family protein"
                     /protein_id="HQR51346.1"
                     /translation="MIQNKNLYAALVMAALLGGCGSSPKPSFYTLSSESVPTADAKAS
                     YTIAVGPLSLPGMLDRPQLVVRVDANRVEIDEFHRWAGPLQQELVRVVAENVGQALGV
                     PTVLTSTQAGAGSADYRVALDVQRFDTSPGSGVTIEAAWTVRGKSGSQKAGHAMVQEP
                     VATKDYDALVAAHSRALARISRDIARDVQALATANPG"
     gene            1057..1737
                     /locus_tag="PKW44_06895"
     CDS             1057..1737
                     /locus_tag="PKW44_06895"
                     /inference="COORDINATES: protein motif:HMM:NF019500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HQR51347.1"
                     /translation="MANVIHHTGRALAPYPRCFYLFFMLVAMLVLLPFLAESRFGMEM
                     SVLFEFLIMAGAIVALGRSYRMRVIGVLLAVPAVGLRLLAVLHGMEAYFLLAKGATLI
                     FYGFILYHLMKYVFHPDVLSKDKLYGAASAYLLLALAWANLYILLQYFFPGAFAINGT
                     ATVLDMKEVLYFSFSVITTAGFGDITASLFQSRCFVMLEATTGVLYVAILIARLAGSY
                     QPEERSKP"
     gene            1734..2813
                     /gene="ydiK"
                     /locus_tag="PKW44_06900"
     CDS             1734..2813
                     /gene="ydiK"
                     /locus_tag="PKW44_06900"
                     /inference="COORDINATES: protein motif:HMM:NF008216.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HQR51348.1"
                     /translation="MTTASEDLARTVLSVLFIVGLMAASLWIIRPFLPSLIWATMIVV
                     ATWPVMRRVQQVCWGRRWLAVSVMTLLLLLLFVLPFLWAIGALVSNTLDVAEWAKALE
                     GLNLQPPAWLGQLPLVGEMLDAKWREIAGLGLDDLGKKLVPHSQEIVRWTLAKMGSIT
                     VVALQLLLIVVLSAILFSQGEVAAMGLRRFGWRLAGENGDQAVVLAGRAIRGVMLGVV
                     LTAVIQATLGGIGLAVAGVPFAALLTFIMFILSVAQIGVVPVMLCAVFWLYSKDVSGT
                     ATVLLVWTVFVGSIDNVIRPILIKKGADLPLLLVFAGVAGGLLAFGLIGLFVGPILLA
                     VSYTLLDAWVKMDSYDAAHAKRSVE"
     gene            2830..3747
                     /locus_tag="PKW44_06905"
     CDS             2830..3747
                     /locus_tag="PKW44_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HQR51349.1"
                     /translation="MSQHHHHSGHGHVPDVYSRAFAIGIALNVGFVLVEVFFGLRAHS
                     LALLADAGHNLSDVLGLVLAWAGMWAARFRPDARHTYGWQRASIMAALVNALILLVAM
                     GSMAWEAFHRFANPAPIQGQTVILVAAAGVLVNGVTAWLFLSGGRADLNIRGAFLHMA
                     GDALVSLGVVVAGALYLWTGWLWLDPLISIVIALLVVFGTWNLFSQSLHLAFDGVPAG
                     IDLNAVKTCLAGFDGVAEVHDLHIWAISTSETAMTVHLLLPGGHPGDAFFVDVTEALH
                     HPSGIEHATIQIEVGDTGQPCRLATHVTA"
     gene            complement(3744..>4465)
                     /locus_tag="PKW44_06910"
     CDS             complement(3744..>4465)
                     /locus_tag="PKW44_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HQR51350.1"
                     /translation="LPGFLFKTQANGDPNHRDRIAFFRVCSGQYTPGMKVKHRRSGKE
                     MKLANAVTFMANERARMEEAFAGDIIGIHNHGNLQIGDVLTEGEALTFKGIPYFAPEL
                     FSRARLRDPLKAKQLQKGLRQLGEEGAVQVFEPLADTAALLGAVGQLQFEVVEHRLKS
                     EYGVDAIFERAGIHTARWVTCEDNAHLNEFARANQARLAKDVDGNLVYLADNHVNLSL
                     AMERWPKVRFHATREHGQKLD"
BASE COUNT          722 a         1302 c         1470 g          971 t
ORIGIN      
        1 aaaatacatg gtgactgggc gatgattggc gagcgggtca acgccgttct gtccaggatc
       61 gagaaaatgc cgattggcga agtcattgaa gatgcgcgca agacgctggc cacgctgaac
      121 gataccctgg acaagttcgg caagctggcg cagcggctgg atagcgaagt ggctccggag
      181 gccgtcgcca ccctggcttc ggccaagcat gcggcggaca acatggagaa aatgctggct
      241 gcagattcgc cgatgcagga ggacatgcgt gcggcactgc aggagctgac gcgcgccgcc
      301 gagtcgatgc gcgtactgac cgattatctg gaaacccatc ccgaggccct gatccgcggc
      361 aagaagaagg aggaatgacc gtgatccaaa acaaaaatct gtatgccgcg ctcgtgatgg
      421 ccgccttgct tggtgggtgc ggcagttcgc ccaagccgag cttctatacc ctcagcagcg
      481 aaagcgtgcc gactgccgat gccaaggctt cctataccat cgcggtcggg ccgctgagcc
      541 tgccgggcat gctggatcgt ccgcagttgg tagtgcgggt ggatgccaac cgggtggaga
      601 tcgatgagtt ccaccgctgg gcgggcccgc tccagcagga actggtgcgc gtcgtcgccg
      661 agaacgtggg gcaggcgctc ggggtgccga cggtgctgac ctccacccag gcgggtgccg
      721 gtagcgccga ttaccgcgtg gcgctggacg tacagcgctt cgataccagt cccggcagcg
      781 gcgtcaccat cgaggcggct tggaccgtgc gcggtaaaag cggtagccag aaggccgggc
      841 atgccatggt gcaggagccg gtggcgacga aggattacga cgccctggtg gcggcgcaca
      901 gccgcgcgtt ggcgcgtatc agccgcgaca tcgcgcggga cgtgcaggcg ctggcaacgg
      961 ccaatcccgg gtaaacggtg caggctgtcc tgacctgcgt agcccaacac cccgccgttg
     1021 gcggcggggt gttcagtctc attaagggac tccgccatgg ctaacgtgat acaccatacc
     1081 ggacgcgcct tggcgcccta tccgcgctgc ttctatctgt ttttcatgct ggtcgccatg
     1141 ctggtgttgc tgccgtttct ggccgaaagc aggttcggca tggagatgag tgtcctgttc
     1201 gaatttctga ttatggcggg tgccatcgtt gctctcggtc gttcctacag aatgcgtgtt
     1261 atcggcgtac tgcttgccgt tccggctgtc ggcttgcggt tgctggccgt gctacacggc
     1321 atggaggcat attttctgct ggccaagggg gcgacgctca tattttatgg ctttattctg
     1381 taccacttga tgaaatatgt gttccacccg gacgtgttga gcaaggacaa actgtatggg
     1441 gcggcttcgg cctatctgct gcttgccctg gcatgggcca atctctatat attgttgcaa
     1501 tacttctttc cgggcgcctt cgccatcaac gggacggcaa ctgtactgga catgaaagag
     1561 gtactctatt tcagtttttc cgtcatcacc accgcgggtt ttggcgacat cacggcgtca
     1621 ctgttccagt cgcgctgttt cgtcatgctg gaagcgacaa cgggggtgct ctacgttgcc
     1681 atcttgattg cccgtctggc tggcagctat caacctgaag aaagatcaaa gccatgacaa
     1741 ccgcgtcaga agatctcgcc cgtaccgtgc tctcggtgtt gttcattgtc gggctgatgg
     1801 ccgcgtcgtt atggatcatt cgtccgtttc tgccttcgct gatctgggcg accatgatcg
     1861 tggtcgccac ctggccggtc atgcgccgcg tgcagcaggt gtgctgggga cggcgctggc
     1921 tggcggtgtc ggtcatgacc ctgctactgc tgctgctgtt cgtgctgcct ttcctgtggg
     1981 ccattggcgc gctggtttcc aataccctgg atgtcgcgga atgggccaag gcgctcgagg
     2041 ggctcaattt gcagccgccg gcctggctgg gacagttgcc gctggtggga gagatgctgg
     2101 atgcgaagtg gcgggagatt gcgggccttg gactggatga tctcggcaag aagctcgttc
     2161 cccattccca ggaaatcgtg cgctggacgc tggccaagat gggcagcatt accgtggtcg
     2221 cgttgcaact gttgttgatt gtggtgctgt cggccatcct gttttcccaa ggagaagtcg
     2281 ccgctatggg cttgcgccgc ttcggctgga ggctggccgg ggagaatggg gatcaagcgg
     2341 tggtgctggc cgggcgagcg atccgcggcg tcatgctggg ggttgtgctg actgccgtca
     2401 ttcaggcaac gctcggtgga atcgggctgg ccgttgcagg ggtgccgttt gccgctttac
     2461 tgacctttat catgttcata ctttcggtgg cacagatcgg cgtggtgccg gtgatgctgt
     2521 gcgcggtgtt ctggctatat tccaaggatg tcagcggcac ggcgaccgtc ctgctggtat
     2581 ggacggtgtt tgtcggcagt atcgacaatg tcatccgccc catcctcatc aaaaagggcg
     2641 cggatcttcc cttgctgctg gtgtttgccg gcgtcgccgg cggcctgctg gctttcggcc
     2701 tgatcggttt gtttgtcggc cccatcctgc tggccgtgag ctacaccctg ctcgacgcct
     2761 gggtcaagat ggattcctac gatgccgcgc atgccaaaag gtccgttgag tagctgactc
     2821 gaggcgcatt tgagccaaca ccaccatcat tccgggcacg gccatgtgcc ggacgtctac
     2881 agccgggcgt tcgccattgg catcgcgctc aatgtgggct tcgtgctggt cgaggtattc
     2941 tttggcttgc gcgcgcactc gctggcgctg ctggccgatg ccgggcacaa cctgtccgac
     3001 gtgctggggc tggtgctggc ctgggccggc atgtgggcgg cgcgtttccg cccggatgcg
     3061 cgccacacct atggctggca gcgtgcctcc atcatggcgg cgctggtcaa tgcgctgatc
     3121 ctgctggtgg cgatgggcag catggcgtgg gaggcgttcc accgctttgc caatccggcg
     3181 ccgatccagg gccagacggt gatcctggtg gccgctgccg gcgtgctggt caacggcgtg
     3241 acggcctggc tgtttctttc cggcggccgc gccgacctca acatccgcgg cgctttcctg
     3301 catatggcgg gcgatgccct ggtgtcgctg ggggtggttg tggcgggcgc gctttacctg
     3361 tggaccggct ggctgtggct ggatccgttg ataagtatcg tgatcgcatt gctggtggtg
     3421 ttcggcacat ggaatctgtt cagccagtcg ctgcatctgg ccttcgacgg cgtgccggcc
     3481 ggcatcgacc tgaatgcggt caaaacctgt ctggccggat tcgacggcgt ggccgaagtg
     3541 catgacctgc acatctgggc gatcagcacc tcggaaaccg cgatgaccgt gcacctgctg
     3601 ctgccaggcg gccatcctgg agatgccttt ttcgtcgatg tgaccgaagc cctgcaccac
     3661 ccctccggca tcgagcacgc cactatccag atcgaagtcg gcgacaccgg ccagccgtgt
     3721 cggctggcga cccacgtaac ggcttaatcc agcttctggc cgtgttcgcg ggtggcgtgg
     3781 aaacgcacct tcggccagcg ctccatggcc aagctgaggt tgacgtggtt gtcggccaga
     3841 tacaccaggt tgccgtccac gtccttggcc agtcgtgcct gattggcgcg ggcgaattcg
     3901 ttgaggtggg cgttgtcctc gcaggtcacc cagcgtgccg tgtggatgcc ggcgcgttcg
     3961 aagatggcat ccacgccgta ttccgatttc agccggtgct cgacaacttc aaactgcaac
     4021 tggccaaccg cacccaatag cgccgccgta tcggccagcg gctcgaacac ctgtaccgcg
     4081 ccttcctcgc ccagctgacg caggcctttc tgcagctgct tggccttgag ggggtcgcgc
     4141 aaccgcgcgc ggctgaatag ttccggcgcg aagtaaggga tgcccttgaa agtgagcgcc
     4201 tcgccctcag tcagcacatc gccgatctgc aggttgccgt ggttgtggat gccaatgata
     4261 tcgccggcaa acgcttcctc catgcgcgcc cgttcgttgg ccatgaaggt gacggcgttg
     4321 gccagcttca tttccttgcc gctgcggcgg tgcttgacct tcatccccgg cgtgtactga
     4381 ccggagcaga cgcggaagaa ggcgatacgg tcacggtgat tgggatcacc attggcctgg
     4441 gtcttgaaca agaagccggg caaag
//
LOCUS       DAOMFA010000131         4425 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_2676_length_4425_cov_5.721281, whole genome shotgun sequence.
ACCESSION   DAOMFA010000131 DAOMFA010000000
VERSION     DAOMFA010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 4425)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4425)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4425
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2676_length_4425_cov_5.721281"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..906)
                     /locus_tag="PKW44_06915"
     CDS             complement(<1..906)
                     /locus_tag="PKW44_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HQR51351.1"
                     /translation="MANEVCFRTLHLPNPGALATYRSIGGYDTLKRIVTDKVPATEII
                     SQVKLSGLRGRGGAGFPTGLKWSFMPRYFDGTKYLVCNTDEGEPGTFKDLDIIRYNPH
                     QLIEGMAIAAYTLGARVGYNYIHGEIWEDYMFFEKALDEARAAGLLGENLFGTNFSFD
                     LYAHHGYGAYICGEETALIESIEGKKGQPRFKPPFPASFGVFGKPTNVNNTESYASIP
                     FILQHGGQAFMDLGVANSGGTKLFAVSGHVEKPGNFEVSMGIPFPELLALAGGVWRGR
                     QLKAVIPGGPSTAVMPAAAIMGATMD"
     gene            complement(906..1379)
                     /gene="nuoE"
                     /locus_tag="PKW44_06920"
     CDS             complement(906..1379)
                     /gene="nuoE"
                     /locus_tag="PKW44_06920"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HQR51352.1"
                     /translation="MLSQESLARIDKELSKYPADRRQSAVMSALRIAQDELGWLSKDT
                     IAFIADYLGIPAIAALEVATFYNMYDLEPVGKYKITVCTNISCMLRESDKIVGHLEHR
                     LGIGFNETTPDGKFTLKEGECMGCCGGAPLFHINNKQMCEHLTPEKVDEILEGLK"
     gene            complement(1379..2632)
                     /locus_tag="PKW44_06925"
     CDS             complement(1379..2632)
                     /locus_tag="PKW44_06925"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014191758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HQR51353.1"
                     /translation="MAEIRNYTMNFGPQHPAAHGVLRLVLELDGEVIERADPHIGLLH
                     RGTEKLAEQRTFLQSVPYMDRLDYVSMMSNEHAYVMAIEKLMGIEVPERAQYIRVMFD
                     EITRILNHLLWLGAHALDVGAMTVFLYAFREREDLFDIYEAVSGARMHAAYYRPGGVY
                     RDLPDRMPQYQENSFRGKDEIKALNDNRQGSVLDFIEDFTNRFPAYVDEYETLLTDNR
                     IWKQRTVGIGVVSPERALALGLTGPMLRGSGFAWDLRKKQPYEVYDRLDFDIPVGVNG
                     DSYDRYLVRMEEFRQSNRIIKQCIDWLRRNPGPVIAADSKIAPPPREGMKQDMEALIH
                     HFKLFTEGFHVPAGEAYAAVEHPKGEFGIYLVSDGANKPYRLKIRAPGFAHLSALDEM
                     TRGHMIADLVAIIGTQDIVFGEIDR"
     gene            complement(2625..3221)
                     /locus_tag="PKW44_06930"
     CDS             complement(2625..3221)
                     /locus_tag="PKW44_06930"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HQR51354.1"
                     /translation="MSPRLEKLSASLKTALADQLVVMHSHVGEITIEASPADLVTVCS
                     TLRDHPELKFEQLIDLCGMDYSGYGEGAWQGARFAVVYHLLSVTLNQRVRLKVFAADD
                     DFPVLASVVGVWPAANWFEREAFDLFGLMFEGHPDLRRILTDYGFVGYPFRKDFPMIG
                     NVEMRYDPDQQRVIYQPVSIEERNNVPRIIRDEGFHHG"
     gene            complement(3231..3707)
                     /locus_tag="PKW44_06935"
     CDS             complement(3231..3707)
                     /locus_tag="PKW44_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018508319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HQR51355.1"
                     /translation="MGIEGLMEKGFVTTTLDTVINYTRTGSMWPMTFGLACCAVEMMH
                     AGASRYDLDRFGIVFRPSPRQSDVMIVAGTLVNKMAPALRKVYDQMAEPRWVISMGSC
                     ANGGGYYHYSYAVVRGCDRIVPVDVYVPGCPPTAEALLYGLIQLQNKIKRTNTIAR"
     gene            complement(3698..4054)
                     /locus_tag="PKW44_06940"
     CDS             complement(3698..4054)
                     /locus_tag="PKW44_06940"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018508318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HQR51356.1"
                     /translation="MLENYFPILLFILVGLGVGIASIAAGKIVSPSRPDSEKNSPYEC
                     GFEAFEDARMKFDVRYYLVAILFILFDLEIAFLFPWAVVIREIGLFGFASMMVFLGIL
                     VVGFVYEWMKGALEWE"
     gene            complement(4073..4157)
                     /locus_tag="PKW44_06945"
     tRNA            complement(4073..4157)
                     /locus_tag="PKW44_06945"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4121..4123),aa:Leu,seq:gag)
     gene            complement(4197..>4425)
                     /gene="secG"
                     /locus_tag="PKW44_06950"
     CDS             complement(4197..>4425)
                     /gene="secG"
                     /locus_tag="PKW44_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HQR51357.1"
                     /translation="GSLFGVSGSANLLSRATAAMVTVFFITSVTLAYLASHGNKHASV
                     VKLQQTTAPVADKAADAVKPAEPSKAKDVPQ"
BASE COUNT          921 a         1316 c         1309 g          879 t
ORIGIN      
        1 atccatggtg gcacccatga tggccgccgc cggcatcacc gcggtggaag gcccgccggg
       61 aatcaccgcc ttcaattgac gtccgcgcca cacgccgccg gccagtgcca gcagctccgg
      121 gaacgggatg cccatgctga cttcgaagtt gcctggcttt tccacatggc cagacacggc
      181 gaacagcttg gtaccgccgg aattggctac gcccaggtcc atgaacgcct ggccgccgtg
      241 ctgcaggata aacgggatag aggcgtagga ctcggtgttg ttgacgttgg tcggcttgcc
      301 gaacacgccg aagctggccg ggaacggcgg cttgaagcgc ggctgtcctt tcttgccctc
      361 gatggactcg atcaacgcgg tttcctcgcc gcagatataa gcgccgtagc catggtgggc
      421 gtacagatca aaagaaaaat tggttccaaa caggttttcg ccgaggagtc cggcggcgcg
      481 cgcttcgtcc agcgctttct cgaaaaacat gtagtcttcc cagatttcgc cgtggatgta
      541 gttgtagccg acgcgcgcac ccagcgtata ggccgcgatg gccatgcctt cgattagctg
      601 gtgcgggttg tagcggatga tatcgaggtc cttgaaagtc cccggctcgc cctcgtcggt
      661 gttacatacc agatacttgg tgccgtcaaa ataacgtggc atgaagctcc acttgaggcc
      721 agtggggaag cccgcaccgc cacggccgcg cagcccggac agcttgacct ggctgataat
      781 ttctgtcgcc ggcaccttgt ccgtcacgat acgcttgagc gtatcgtagc cgccgatgct
      841 gcggtaggtt gccagcgcac ccggattggg caggtgcagt gtcctgaagc aaacctcatt
      901 agccattact tgaggccctc cagaatttca tccacctttt ccggcgtcag gtgttcgcac
      961 atctgcttgt tgttgatgtg gaacagcggt gcaccgccgc agcagcccat gcactcgccc
     1021 tccttgaggg tgaacttgcc gtccggcgtg gtctcgttga agccaatgcc gaggcgatgc
     1081 tccaggtgcc cgacgatctt gtccgactcg cgcagcatgc aggagatatt ggtacaaacg
     1141 gtgatcttgt acttgcccac cggctccagg tcatacatgt tgtagaacgt tgccacctcc
     1201 agcgcggcga tggccgggat gccgagatag tcggcaatga aggcgatggt gtccttggac
     1261 agccagccca gttcgtcctg cgcgatgcgc aatgccgaca tcaccgcgga ctggcgacgg
     1321 tcggccggat acttgctcaa ctctttgtcg atcctggcga gagattcttg cgacaacatc
     1381 agcggtcaat ctccccgaaa acaatgtcct gcgtaccgat aatggccacc agatcggcga
     1441 tcatgtggcc gcgcgtcatc tcgtcaagcg cggaaaggtg ggcaaagccg ggcgcgcgaa
     1501 ttttcaggcg gtagggcttg ttggcgccat ccgacaccag atagatgccg aactcgccct
     1561 tgggatgctc caccgcggca taggcctcgc cggccgggac gtggaagccc tcggtaaaca
     1621 gtttgaaatg gtggatcagc gcctccatgt cctgcttcat gccctcacgc ggcggcgggg
     1681 cgatcttgct gtcggccgca atgaccgggc caggattcct gcgcagccaa tcgatacact
     1741 gtttgatgat gcggttggac tggcggaact cttccatgcg caccaggtag cggtcgtagg
     1801 aatcaccatt gacaccaact gggatatcga aatcgaggcg atcgtagact tcatagggct
     1861 gcttcttgcg caggtcccag gcgaacccgg agccacgcag catggggccg gtcaggccca
     1921 gcgccagcgc acgttccggc gacaccacac cgatgccgac ggtgcgctgc ttccagatac
     1981 gattgtcggt aagcagggtt tcgtattcgt ccacgtaggc cgggaagcgg ttggtgaaat
     2041 cctcgataaa atccagcacc gagccctggc ggttgtcgtt gagcgccttg atctcatcct
     2101 tgccgcggaa actgttttcc tgatactgcg gcatgcggtc cggcaggtcg cggtagacgc
     2161 cgcctggacg gtaataggcc gcgtgcatgc gggcgccgga tacggcctcg taaatgtcga
     2221 acaaatcctc gcgctcacgg aaggcataca gaaataccgt catcgccccc acgtccagcg
     2281 catgtgcgcc cagccacagc aggtgattga ggatgcgggt aatctcgtcg aacatgacgc
     2341 ggatatactg agcgcgctct ggtacttcaa tgcccatgag cttttcgatc gccatcacgt
     2401 aggcatgctc gttggacatc atggagacgt aatccaggcg atccatgtaa ggcacggatt
     2461 gcaggaaagt acgctgctcc gcaagcttct cggtgccgcg gtgcagcagt ccgatgtgcg
     2521 ggtcggcgcg ttcgatcacc tcaccgtcca gttccagcac caggcgcagc acgccatgcg
     2581 cggcaggatg ctggggaccg aaattcatgg tgtagttgcg aatctcagcc atgatggaag
     2641 ccctcgtcgc gaatgatgcg cggcacgttg ttgcgttcct cgatcgatac cggctggtag
     2701 atgacgcgtt gctgatcggg gtcataacgc atctcgacgt tgccgatcat ggggaaatcc
     2761 ttgcggaacg gatagccgac aaaaccataa tcggtaagga tgcggcgcaa gtcaggatgg
     2821 ccctcgaaca tcaggccgaa caggtcgaac gcctctcgct cgaaccagtt ggcagccggc
     2881 cacaccccga ccaccgaggc cagtaccgga aaatcgtcgt ccgcggcaaa caccttgagg
     2941 cgcacgcgtt ggttgagcgt caccgatagc aggtggtaca ccacggcaaa gcgcgcgccc
     3001 tgccaggcac cctcgccata gccgctgtaa tccatgccgc acaggtcgat cagctgctcg
     3061 aacttgagtt ccgggtggtc gcgcagcgtg ctacataccg tgactaggtc ggccgggctg
     3121 gcttcaatgg tgatttcgcc gacgtgcgag tgcatgacga ccagttggtc agccaaggca
     3181 gtcttcaggc ttgcggaaag tttttccagg cgagggctca tttttttgtt ttacctggca
     3241 atcgtattgg tgcgcttgat cttgttctgt agctggatga ggccgtacag cagcgcttcg
     3301 gccgtcggtg ggcagcccgg cacatagaca tccaccggca cgatacggtc acaaccgcgc
     3361 accacggcat aggaatagtg gtaatagcca ccgccgttgg cacaggaacc catagagatc
     3421 acccagcgcg gctcggccat ctggtcgtat accttgcgca gcgccggagc catcttgttg
     3481 accagggtgc cggcgacgat catcacgtcg gactggcgcg gactggggcg gaacacgatg
     3541 ccgaagcggt caaggtcgta gcgcgaagca ccggcatgca tcatttccac cgcgcagcag
     3601 gccagaccga aggtcatcgg ccacatggag ccggtgcgag tgtagttgat gacggtgtcc
     3661 agggtagtcg taacaaaccc cttttccatc aggccttcta ttcccattcc agggctccct
     3721 tcatccactc gtagacgaag cccaccacca gaataccgag gaacaccatc atggaggcaa
     3781 agccgaacag gccgatttcg cggatcacca ctgcccaagg aaaaaggaag gcgatttcca
     3841 ggtcgaaaag aatgaacagg atggcgacca ggtaatagcg cacatcgaac ttcatgcgtg
     3901 cgtcttcaaa cgcctcgaag ccacattcgt aaggggagtt tttttcgctg tcggggcggc
     3961 tgggggatac gatcttaccc gcggcaatag aggcaatacc gacgccaagc ccgacaagga
     4021 tgaataacaa aatcggaaaa taattttcca gcacgtcctg gatctttcat gttggtgccg
     4081 acgatgagac tcgaactcat acgactttcg tcactacccc ctcaagatag cgtgtctacc
     4141 aattccacca cgtcggcatc gcgggcgcct tgcgccccaa aacttcttac caaaaactac
     4201 tgaggaacat ccttggcctt ggaaggctcg gccggcttga cggcatcagc ggccttgtct
     4261 gccactggcg cggtcgtctg ctgcagcttg accacgctcg catgcttgtt accgtggctg
     4321 gcgagatagg ccagtgtcac gctggtaatg aagaacaccg tgaccatagc tgccgtcgcg
     4381 cggctcagca agttagccga cccggaaact ccgaacaggc tcccg
//
LOCUS       DAOMFA010000132         4351 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_2753_length_4351_cov_5.326117, whole genome shotgun sequence.
ACCESSION   DAOMFA010000132 DAOMFA010000000
VERSION     DAOMFA010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 4351)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4351)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4351
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2753_length_4351_cov_5.326117"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..489)
                     /locus_tag="PKW44_06955"
     CDS             complement(<1..489)
                     /locus_tag="PKW44_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HQR51358.1"
                     /translation="MPGLVDTHCHLDAAEFDADRDEVVQAAWDAGVTAIVVPAVERGN
                     FFVVEALCNATPRCLPAYGIHPMYVDRASQTDLTVLEALLARGQCVAVGEIGLDFFVE
                     GYDRELQEWFFVEQLKLAHQYDLPVLLHIRRAQDAILKHLRRIKVRGGIAHAFNGSRQ
                     QAD"
     gene            complement(473..1558)
                     /gene="ribD"
                     /locus_tag="PKW44_06960"
     CDS             complement(473..1558)
                     /gene="ribD"
                     /locus_tag="PKW44_06960"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HQR51359.1"
                     /translation="MFSAVDHIHMSHALRLAERGLHTTMPNPRVGCVIAKDGTVAGEG
                     WHERAGEPHAEIHALRQAGEQARGATVYVTLEPCSHHGRTPPCAEALVAAGVARVVAA
                     MRDPNPRVAGNGLELLRSKGIEVATGLMAVQARELNIGFILRMERGRPWVRSKIAASL
                     DGRTALENGVSQWITGAAARRDVQRWRARSCAILTGVGTVLADDPQLTVRDVDTGRQP
                     LRVVVDSRLRTPPSARIAQGGSTLLVAAMEDAAKTDTLRAAGAEVLALPGVDGRVDLP
                     ALFEELARREINEILVEAGTGLNGPLLQAGLIDELLLYYAPTLLGDAAHGMFALQPLR
                     EMSQRVELDILELDRIGQDIRLRARPR"
     gene            complement(1558..1959)
                     /locus_tag="PKW44_06965"
     CDS             complement(1558..1959)
                     /locus_tag="PKW44_06965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR51360.1"
                     /translation="MEQSPEPSLAFLKWAALAFAAVTVLITVVFFGWWAMKEHFVFGH
                     DRYDPVRWMAPMTRQSYECQRGDMVLDVRQRLLQPGMRKSEVTTLLGRPSWEETGQFE
                     YDLGVCLWVVHGLRLYFDPQDRLVHSAIVQH"
     gene            complement(1963..2451)
                     /gene="nrdR"
                     /locus_tag="PKW44_06970"
     CDS             complement(1963..2451)
                     /gene="nrdR"
                     /locus_tag="PKW44_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HQR51361.1"
                     /translation="MKCPFCGADDTQVIDSRVNDDGDSIRRRRRCASCEKRFTTYETA
                     DLQLPQVVKQNGKREEFSRDKLRLSFTRALHKRPVPTEYVDRAIDHIVQGILSRGERE
                     INARELGEAVMQELRRLDKVAYIRFASVYRSFQDVDDFREAIRDLNQAHRHNTDVPES
                     AE"
     gene            complement(2469..3719)
                     /gene="glyA"
                     /locus_tag="PKW44_06975"
     CDS             complement(2469..3719)
                     /gene="glyA"
                     /locus_tag="PKW44_06975"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018749062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HQR51362.1"
                     /translation="MFSASQTIAKFDPELFQAMEAERQRQEDHIELIASENYTSPAVM
                     EAQGSVLTNKYAEGYPGKRYYGGCEFVDIAEQLAIERAKKLFGAEYANVQPHSGSQAN
                     AAVYLSVLKPGDTILGMSLAHGGHLTHGASVNFSGKVFNAVTYGLHPDTEEIDYDEVQ
                     RLANEHKPKMIVAGASAYALVIDWKRFRKIADSVGAYLFVDMAHYAGLVAAGLYPNPV
                     GIADFVTSTTHKTLRGPRGGLILSSAEHEKALNSAIFPNIQGGPLMHVIAAKAVAFKE
                     AASKEFKHYQEQVMANARVMATVLVERGLRIVSGRTESHVFLVDLQPKNLTGKDAEAL
                     LGRAHITVNKNAIPNDPQKPFVTSGIRIGTPAMTTRGFKEAEAEKLANLIADVLDAPN
                     DEAVITRVLDEVKALTAKFPVYGA"
     gene            complement(3798..4307)
                     /locus_tag="PKW44_06980"
     CDS             complement(3798..4307)
                     /locus_tag="PKW44_06980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR51363.1"
                     /translation="MPEQASAENNLKQHLMLDGEVEYEQAIDQVIERAQRTLHIFDLD
                     LARGGYAGLGRYNALRDFLLRNHGNRLVIVLHDTDHITRYCPRLMSLLRIFSHSISIH
                     ATEDHARSASDPFVIADQAHYVHRFHSSGARFLLALHDHQGASQLEERFGQLVEASQP
                     ASAVHNLGL"
BASE COUNT          755 a         1349 c         1394 g          853 t
ORIGIN      
        1 atccgcttgc tgacggctgc cgttgaaagc atgggcgatg ccgccgcgca ccttgatgcg
       61 gcgcagatgc ttgaggatgg cgtcctgcgc gcggcggata tgcaacagca ccggcaggtc
      121 gtattggtgc gccaacttca gctgttcgac gaaaaaccac tcctgcaact cgcgatcata
      181 gccctcgacg aaaaaatcca ggccgatttc acccacggcg acacattggc ccctggccaa
      241 taacgcttcc agcaccgtca gatcggtttg cgacgcacga tcaacgtaca tgggatgaat
      301 gccgtaagct ggcaggcagc gcggcgtagc gttgcatagc gcttccacga cgaaaaaatt
      361 gccgcgttcc actgccggca ccacgatagc cgtgacaccg gcatcccatg cggcctgaac
      421 cacttcgtcg cggtcggcgt cgaactctgc cgcgtccagg tggcagtgtg tatcaacgag
      481 gccgggcacg gagacgaatg tcctgtccga tgcggtcgag ttcgaggatg tcgagttcga
      541 cgcgctgcga catttctcgt aacggctgca aggcgaacat gccgtgcgcg gcgtcaccca
      601 gcagagtcgg cgcgtaatac agcagcagtt cgtctatcag cccggcctgc agcagggggc
      661 cgttcagtcc agtgccggct tcgaccagga tttcattgat ctcgcgccgc gccaactcct
      721 cgaacagtgc cggcaagtcc acgcggccat cgacgcccgg cagtgccagc acttcggcgc
      781 ctgcggcacg caaggtatcg gttttggcgg catcctccat cgcggccacc agcagcgtac
      841 tgccgccctg cgcgatgcgc gccgatggcg gggtgcgcaa ccggctgtcc accaccaccc
      901 gtagcggttg acggccggtg tcaacgtcgc gcaccgtcag ttgcggatcg tccgccagca
      961 ccgtgcccac gcccgtcagg atggcacagg agcgcgcgcg ccagcgctgt acatcgcgcc
     1021 gcgccgccgc gccggtaatc cactggctga cgccgttctc cagcgcggtg cgcccgtcca
     1081 gactggcggc gatcttgctc ctgacccacg gccgcccgcg ctccatgcgc aagatgaagc
     1141 cgatattgag ttcgcgcgcc tgcaccgcca tcaggccggt ggcaacctcg atgcccttgc
     1201 tgcgcaacag ctccagtcca ttccccgcca cacggggatt ggggtcgcgc atcgcggcta
     1261 ccacgcgggc gacgccagcg gccaccagcg cttcggcaca aggcggcgtg cgcccgtgat
     1321 ggctgcaagg ctccagcgtg acgtaaaccg tggcgccgcg cgcttgctcg ccggcctggc
     1381 gcaaggcatg gatttcggca tgcggctcgc cggcgcgctc gtgccagcct tcgcctgcca
     1441 cggtgccatc cttggcgatg acgcagccga cgcgcgggtt gggcatggtg gtatggaggc
     1501 cgcgctctgc gaggcgtagc gcgtgggaca tgtggatgtg gtcgaccgcg gagaacatca
     1561 gtgctgcaca atagcgctgt gcaccagcct gtcctgcgga tcgaaataca gccgcaggcc
     1621 atggactacc cacaggcaga cgcctaaatc gtattcgaac tggccggttt cttcccagga
     1681 tgggcggccc agcaaggtgg tgacttcaga cttgcgcatg cccggttgca gcaggcgttg
     1741 gcgcacgtcc agtaccatgt cgccgcgctg gcattcgtaa gactgcctgg tcatcggcgc
     1801 catccagcgc accgggtcgt agcggtcatg gccgaaaacg aagtgttctt tcatcgccca
     1861 ccagccgaag aacaccacgg taatcagcac cgtcaccgcg gcaaacgcca gggcagccca
     1921 tttcaggaac gccagtgacg gttccggcga ctgctccatt gcttattctg cggattccgg
     1981 cacatccgtg ttgtgccgat gtgcttgatt gaggtcgcgg atggcttcgc ggaaatcgtc
     2041 cacgtcctgg aagctgcggt agaccgaggc aaagcggata taggccacct tgtccagccg
     2101 ccgcagttcc tgcatgaccg cttcgcccag ttcgcgtgcg ttgatttcgc gttcgccgcg
     2161 gctgaggatg ccctgcacga tatggtcaat agcgcggtcg acgtattcgg tcggcaccgg
     2221 acgcttgtgc aaggcgcggg tgaaggacag gcgcagcttg tcgcgcgaaa actcctcgcg
     2281 cttgccgttc tgcttgacta cctgcggcag ctgcagatcg gcggtttcgt aggtggtgaa
     2341 gcgcttctca caggaagcgc agcgccgtct gcggcggatg ctgtcgccat cgtcattgac
     2401 gcgggagtcg atgacctggg tatcgtcggc accgcagaac gggcatttca tgggcggcgc
     2461 ttagtggctc aggccccgta aaccgggaat tttgccgtca gcgccttgac ttcgtccagg
     2521 acgcgggtga tcaccgcctc gtcattgggt gcgtccagca cgtcggcgat caggttggcg
     2581 agcttctcgg cttcggcttc cttgaagccg cgcgtggtca tcgccggcgt gccgatgcgg
     2641 atgccgctgg tgacgaaggg tttctgcggg tcgttgggga tggcattctt gttgacggta
     2701 atgtgggcac ggccgagcaa agcctcggcg tccttgccgg tcagattctt gggctgcagg
     2761 tcgaccagga acacgtggct ctcggtgcgg ccggagacga tgcgcaagcc gcgttccacc
     2821 agtaccgtag ccatcacgcg ggcgttggcc attacctgct cctggtagtg cttgaattcc
     2881 ttgctggcgg cttccttgaa cgccacggcc ttggcggcga tgacgtgcat cagcggaccg
     2941 ccctggatat tcgggaaaat cgcagaattc agcgccttct cgtgctcggc ggaagacaaa
     3001 atcaagccac cgcgtgggcc gcgcaaggtc ttgtgggtgg tgctggtgac gaaatcggca
     3061 atgccgacgg ggttcgggta cagccctgca gccaccagtc cggcgtagtg cgccatgtcg
     3121 acgaacaggt aagcgccgac gctgtcggcg atcttgcgga agcgcttcca gtcgatcacc
     3181 agcgcatagg ccgaggcgcc agccacgatc atcttcggct tgtgctcatt ggccaggcgc
     3241 tgcacctcgt catagtcgat ctcctcggtg tcgggatgca ggccgtaggt gacggcgttg
     3301 aacaccttgc cggagaaatt gacggaggca ccgtgggtca ggtggccgcc gtgcgccagg
     3361 gacatgccga ggatggtgtc gcccggctta agcacggaca gatacaccgc ggcattggcc
     3421 tgcgagccgg aatgcggctg tacgttggcg tattcggcgc cgaacagctt cttggcgcgc
     3481 tcgatggcca gctgctcggc gatgtcgacg aattcacagc cgccgtaata gcgcttgccc
     3541 ggatagcctt cggcatactt gttggtcagt accgacccct gcgcttccat caccgccggg
     3601 ctggtgtagt tttccgaggc gatcagctcg atgtggtctt cctggcgctg acgttctgcc
     3661 tccatggcct ggaacagttc ggggtcgaat ttggcgatgg tctgggatgc gctgaacatg
     3721 atggtttccg gggtgaaaaa tgcgtaaatt ctaacataat tcgatgcccg gccgggatgc
     3781 ccgcttctgt ctgccggcta cagtcccagg ttgtgcacgg ccgatgccgg ctgcgatgct
     3841 tccacgagtt gcccgaaacg ttcttccagc tggctggcac cctggtggtc gtggagcgcg
     3901 agcagaaagc gcgccccact gctgtggaag cggtgtacat aatgcgcctg gtcggcgatg
     3961 acgaaagggt cgctggctga tcgggcgtga tcctctgtcg cgtggatgga aatggaatgg
     4021 ctgaagatcc tgagcaggct catcaggcgc gggcagtatc gcgtgatgtg atcggtatcg
     4081 tgtagaacga tgaccagccg gttgccgtga tttcgcaaca gaaagtcgcg cagggcattg
     4141 tagcggccaa gcccggcgta gccgcccctg gcaaggtcga ggtcgaaaat atgcagtgtg
     4201 cgctgtgccc gctcgatcac ctgatcgatc gcctgctcgt attccacctc gccgtccagc
     4261 atgaggtgtt gcttgagatt gttctccgca cttgcttgtt ctggcatgcc cccccccttt
     4321 tttttttatg ctaaaagtcc aagtagcccg c
//
LOCUS       DAOMFA010000133         4321 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_2782_length_4321_cov_5.103141, whole genome shotgun sequence.
ACCESSION   DAOMFA010000133 DAOMFA010000000
VERSION     DAOMFA010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 4321)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4321)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4321
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2782_length_4321_cov_5.103141"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..394
                     /locus_tag="PKW44_06985"
     CDS             <1..394
                     /locus_tag="PKW44_06985"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HQR51364.1"
                     /translation="FTGAVADAENYQDKLMQRFGKRFTQGEYIFREGDQGQEMYFIVS
                     GAVTVYHQGRTLRELGAGEYFGEMAVLSEVRRAADAIVQSRQAEILRVSADNFATLLL
                     QEPGVAMSLLREMARRYRRASGLDVPGQ"
     gene            complement(415..2016)
                     /locus_tag="PKW44_06990"
     CDS             complement(415..2016)
                     /locus_tag="PKW44_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQR51365.1"
                     /translation="MKAQFQMQRQPSQFEIQSLLNLFNAGRLVQSEETARALLKSYPG
                     AFILHNVLGVSLEGQRKFEEAAAVYRKALEIEPKIAEIHFNLGVVLGNLGRTEEAVTS
                     YRKAISLKPDLTVAYFNLGVILQEQGKFEEAVTCYRKAVAQQPGFYEAYGNLGVVLQK
                     QGRLEDAVVNYRKALAISPDPLGYFNLGTALRDQGKHEEAAQSYLKALEMNPNYADAH
                     NNLGEIFRDRGNMDEAIKCYLNAMAINPDHPNANYNMGEFHSLAQRYEEAIPYFERSR
                     FQDYQERAMECLYKIERYDEFREKLKAMVHSGKKSMMLATLSTHHAINFGVPDEYNFC
                     PDPMRFAWHTSIPELAAPDSQLLQEVLETIDRTPIAARMQGRLYSGMQSAGNLLKRPE
                     PAFQQLAALVKQKIVEYREHFANENCELIKSFPKEIDFSSSWYLKMKKGGNLGSHIHE
                     EGWISGCVYLKLPKQKLHVTDAGFEYGTDGDKYPRKHDNFPSHVVVQEVGDIVLFPSS
                     LFHRTIPFHSDEDRVCVAFDLKPAV"
     gene            complement(2089..2538)
                     /locus_tag="PKW44_06995"
     CDS             complement(2089..2538)
                     /locus_tag="PKW44_06995"
                     /inference="COORDINATES: protein motif:HMM:NF019301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HQR51366.1"
                     /translation="MLEKLKNLLLTLRCWIVAASPLADLALRLWVANVFWKSGLTKIA
                     SWDSTVALFENEYAVPLLPPEIAAYMGTATELSMPVLLVLGLGTRFGAFVLFVFNIIA
                     VVSYPDLSEAGRYNHVQWGLMILIIFAHGPGKLSLDYWLSCRFLRVE"
     gene            complement(2542..3324)
                     /locus_tag="PKW44_07000"
     CDS             complement(2542..3324)
                     /locus_tag="PKW44_07000"
                     /inference="COORDINATES: protein motif:HMM:NF021364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding domain-containing protein"
                     /protein_id="HQR51367.1"
                     /translation="MPSLREVQERFRDAVFEAPHGTPDFIVPGRLTAEWRMAAYRGSV
                     FGNLRSALRGVYPVTDRLVGEEFFDFAADRFIRDTLSPSGDVHQFGREFAEFLRAFPS
                     AASLPYLPDVARLEWLMHEIHYVAELAPLDLAALAALLAEDHGDLGFSLNPACRLLAS
                     PYPIDLIWQANQSEIAEPEQIDLDSGTARLLVRRRAGALETQRLDAGSYALLEQLHAG
                     ALFSMALQAALAVQADFDAASFLLQQVQLGTLVAFHQSEREG"
     gene            complement(3308..4153)
                     /locus_tag="PKW44_07005"
     CDS             complement(3308..4153)
                     /locus_tag="PKW44_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018412733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
                     /protein_id="HQR51368.1"
                     /translation="MSADDLSTIPVRVGIGLRAPHIREFLDTLPDVGWVEVHSENYFG
                     DGGQPLALLEQVRQHYPVSLHGVGLSLGSSDELNTEHLRRLKRLVDHITPGFVSDHIS
                     WSSVGGRYFNDLLPIPYTEESLNLVCRHVEQTQDYLGRRILAENASSYLQFSHSTIPE
                     WEFVEQVANRTGCGILLDINNVYVSASNHGFDASAYLRAIPKEMVQEFHLAGYTDTGS
                     LLLDTHGAPVFDAVWVLYGEALARFGAIPTLIEWDTDIPELAVLLDEAGKAAHMMEAE
                     RALAA"
     gene            complement(4153..4311)
                     /locus_tag="PKW44_07010"
     CDS             complement(4153..4311)
                     /locus_tag="PKW44_07010"
                     /inference="COORDINATES: protein motif:HMM:NF021559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2282 domain-containing protein"
                     /protein_id="HQR51369.1"
                     /translation="MVKAGKNDCATSTNSCAGTATKDGDKEAWVYVPKGTCSKIVGGT
                     ILADAKKK"
BASE COUNT          864 a         1265 c         1333 g          859 t
ORIGIN      
        1 atttaccggc gccgtggcag atgcggaaaa ctaccaggac aagctgatgc aacgctttgg
       61 caaaagattc acgcagggcg aatatatttt ccgcgaaggg gaccaggggc aggagatgta
      121 tttcatcgta tccggcgcgg tcaccgtcta tcaccaaggc cgcaccctgc gcgaactcgg
      181 tgccggcgaa tactttggtg aaatggctgt cctgagcgaa gtcaggcgcg ccgcagatgc
      241 cattgtacag tcacgtcagg cggagatcct gcgggtcagt gcggacaact ttgccacgct
      301 gctgctgcag gaacccgggg ttgccatgtc cctgctgcgc gaaatggccc gccgctaccg
      361 gcgggccagc ggcttggacg tccccgggca atagcatcca cggagcgccc gaaatcagac
      421 ggcgggcttc agatcaaagg cgacacacac ccgatcctcg tccgagtgga acggaatggt
      481 gcggtggaac agcgacgaag gaaacagcac aatatcgccc acttcctgca ccacgacatg
      541 cgacgggaaa ttgtcgtgct tgcgcgggta tttgtcgcca tcggtgccat attcaaaacc
      601 ggcgtccgtg acatgcagct tctgcttcgg cagcttgagg taaacgcagc cgctgatcca
      661 gccttcttcg tggatgtgcg aaccgaggtt accgcccttt ttcatcttca ggtaccagga
      721 gctgctgaaa tcgatttcct tggggaagga cttgatgagt tcgcagttct cgttggcaaa
      781 atgctcgcgg tattcaacga ttttctgttt gaccagtgcc gccagctgct gaaaagctgg
      841 ctccggccgc ttgagcagat tgccggccga ctgcatgccc gaataaagcc ggccctgcat
      901 gcgtgcggcg atcggggtac ggtctatggt ttccagcacc tcttgcagca actggctatc
      961 cggcgccgcc agctcgggga tgctggtgtg ccaggcaaag cgcatcggat ccgggcagaa
     1021 attgtattcg tccggtacgc cgaagttgat ggcatggtgc gtggataacg tggcaagcat
     1081 catcgacttt ttaccggaat gcaccatggc cttcagcttt tcacggaatt cgtcgtagcg
     1141 ctcgatcttg tacaggcatt ccatggcgcg ctcctggtag tcctggaaac gcgagcgctc
     1201 gaaataaggg atggcttcct cgtaacgctg ggcgaggctg tggaattcgc ccatgttgta
     1261 gttggcgttg ggatggtccg ggttgatcgc catggcgttg aggtagcact tgatggcctc
     1321 gtccatgttg ccgcggtcgc ggaagatttc gcccagatta ttgtgcgcgt cggcgtagtt
     1381 ggggttcatc tccagcgcct tgaggtagga ttgcgccgct tcctcatgct tgccctggtc
     1441 gcgcagcgcc gtccccagat tgaagtagcc cagagggtcc ggactgatcg cgagcgcctt
     1501 gcggtaattg accacagcat cttccagcct gccctgcttt tgcagcacca cgccgaggtt
     1561 gccgtaggcc tcgtagaagc ccggctgctg ggcgaccgcc ttgcggtagc aggtgacggc
     1621 ttcctcgaac ttgccctgct cctgcaggat gacgccgaga ttgaagtagg ctaccgtcag
     1681 atcgggcttg agggaaatcg ccttgcggta gctggtgacg gcctcctccg tccggcccag
     1741 attgcccagc accacaccga gattgaaatg gatttcggca atcttcggct cgatttccag
     1801 cgccttgcgg taaaccgcgg cagcctcctc aaacttgcgc tgcccttcca gcgagacccc
     1861 cagcacattg tgcaggataa acgcgccggg ataggacttg agcagtgctc tggcagtctc
     1921 ttcagactgc accagacggc cggcgttgaa caggttgagc agcgactgga tctcgaactg
     1981 gctgggttgc cgctgcattt ggaattgggc tttcatggcg cgtctctttg acgttattca
     2041 atcccgccaa ttatagtcaa acatcggcta tttgatagag gccagaaatt attccaccct
     2101 gagaaatctg catgacagcc agtaatccag ggacagtttg ccgggaccgt gagcgaagat
     2161 gatcaggatc atcaaacccc attgcacgtg gttgtatcgg ccagcctcgg aaagatcggg
     2221 ataggatact acggcaatga tattgaacac gaatagtacg aaggcgccaa agcgcgtacc
     2281 cagcccgagt accagcagca ccggcatgct gagttcggtg gcggtaccca tataggcagc
     2341 gatttccggc ggcagcaacg gcaccgcata ctcgttctcg aacaacgcca cggtggaatc
     2401 ccagcttgcg atcttggtca ggccggattt ccagaacaca ttggccaccc acagacgcag
     2461 cgccaggtcg gccagcgggc tggcggcgac aatccagcag cgcagggtaa gcagcaggtt
     2521 tttcagtttt tccagcatgg ctcagccctc cctttcagat tggtgaaaag cgactagtgt
     2581 ccccagttgc acttgctgca acagaaagga cgccgcgtcg aaatcggctt gcaccgccag
     2641 cgcggcctgc aaagccatgg aaaacagtgc gccggcgtgc agttgttcca gcaaggcata
     2701 gctgcctgca tccaggcgct gcgtttccag cgcgccggca cggcggcgta ccagcaggcg
     2761 cgccgtgccg ctatcgagat cgatctgttc cggctccgcg atttccgact ggttggcttg
     2821 ccagataagg tctatcgggt agggcgaagc caacagccgg caggccgggt tgaggctgaa
     2881 gccaaggtcg ccgtggtctt ccgccagcag cgcagccaga gcggcgaggt ccagcggtgc
     2941 cagttcggcc acgtaatgaa tttcgtgcat cagccattcc aggcgtgcca cgtccggcag
     3001 gtagggcagg ctggcggcgg acgggaaagc gcgcaggaat tcggcgaatt cgcggccgaa
     3061 ctggtgcacg tcaccgctgg gcgagagcgt gtcccggatg aagcgatccg cagcgaaatc
     3121 gaagaattct tcccccacca ggcggtcggt gacggggtaa accccgcgca gagcggagcg
     3181 caggttgccg aacacgctgc cgcgataggc ggccatgcgc cactcggcag tcaacctgcc
     3241 ggggacaatg aaatccggtg tgccgtgtgg agcctcgaac accgcatcgc ggaagcgctc
     3301 ctgcacttca cgcagcgagg gcacgctccg cctccatcat gtgcgcggcc ttgcctgcct
     3361 cgtccagcag caccgccagt tccggaatat cggtatccca ttcgatcaag gtcgggatag
     3421 cgccgaaacg cgccaacgcc tcgccgtaca gcacccacac cgcatcgaat accggagcac
     3481 cgtgggtatc gagcaacagg ctgccggtgt cggtatagcc agccagatgg aactcctgca
     3541 ccatttcctt ggggatggcg cgcaggtaag cactggcatc gaagccatgg ttgctggcgc
     3601 tgacataaac attattgatg tcgagcagga tgccgcagcc ggtgcggttc gccacctgct
     3661 ccacgaactc ccattccgga atggtggagt ggctgaattg caggtagctg gaggcgtttt
     3721 ccgccaggat gcggcggccg aggtagtcct gtgtctgctc gacgtggcgg cacaccaggt
     3781 tcagcgactc ctcggtgtag gggatgggca gcaggtcgtt gaaatagcgc ccacccaccg
     3841 atgaccagct gatgtgatcg gaaacgaaac cgggggtgat gtggtcgacc aggcgcttga
     3901 ggcgacgcag atgctccgtg ttcagttcat cgctggatcc cagcgacagg ccgacaccgt
     3961 gcagactgac cggataatgt tggcgcacct gttccagcag ggcgagcggc tggccgccgt
     4021 cgccgaaata gttttcactg tggacttcta cccagcctac atccggcaga gtgtcgagaa
     4081 actcgcggat atgcggtgca cgcaggccga tgcccacgcg caccgggatg gtagagaggt
     4141 cgtccgcgga cattacttct tcttggcgtc ggcgagaatg gtaccaccca cgatcttgct
     4201 gcaggtaccc ttgggcacat acacccaggc ttccttgtcg ccgtccttgg tggcggtgcc
     4261 ggcacaggaa ttggtgctgg tggcgcagtc gttcttgccg gccttgacga tgccggcgca
     4321 t
//
LOCUS       DAOMFA010000134         4310 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_2790_length_4310_cov_4.776263, whole genome shotgun sequence.
ACCESSION   DAOMFA010000134 DAOMFA010000000
VERSION     DAOMFA010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 4310)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4310)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4310
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2790_length_4310_cov_4.776263"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            3..761
                     /gene="zapD"
                     /locus_tag="PKW44_07015"
     CDS             3..761
                     /gene="zapD"
                     /locus_tag="PKW44_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HQR51370.1"
                     /translation="MASYDYPFNERIRTLLRLEDLFGKILHYADGKHELNHHCALLGI
                     FQVLDVIDRAELKTDLLQELDRQKIVMENLRGNPAIAEDVLDEVIVDIGETAHVMRNS
                     AGKIGQSLRDNEWLMGIKQRAGIPGGVCEFDVPSYHYWLGLPVDQRQQYLQSWMAPLM
                     PLHDTLRIILHILRGSGTTTKQVAPQGSFQQMLGGAKLAQMLRITIDDDLHCFPEVSA
                     NKYVINVRFNTLELAQKPRQCDFDVDFKLTLCNL"
     gene            complement(1153..1776)
                     /locus_tag="PKW44_07020"
     CDS             complement(1153..1776)
                     /locus_tag="PKW44_07020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR51371.1"
                     /translation="MSVKGAIKGNQTIDYILSAKSGQAMKVSLKTDNGANYFNVLPPG
                     SNDEAIFVGSTDGNEWKGTLQADGEYKVRVYLMRSAARRNEAANYTLTVGITGTPKAS
                     DLGKAPATDVKVKGTAYHATGPLPCRMGNEKPVQCEFGVIRGKPGNAEVHITPPGGLK
                     RVLTFMGDKVTTNPGEKVKAVKQGYDWLVEVNDYEHYTIPDAVISGG"
     gene            2134..2301
                     /locus_tag="PKW44_07025"
     CDS             2134..2301
                     /locus_tag="PKW44_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020182696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HQR51372.1"
                     /translation="MVACPTCGAQTEYTSANPYRPFCSRRCKLIDLGQWATENYRIPE
                     NQKPEPADEEK"
     gene            2303..2746
                     /locus_tag="PKW44_07030"
     CDS             2303..2746
                     /locus_tag="PKW44_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HQR51373.1"
                     /translation="MNKFFAAALAGMCFLIGMAAQAGDSLTVSNAWARATVPGQEVGA
                     AYMELRSAAGATLTGMESPASDSVEIHSMSMQGEQMQMRMLEALPLPAGKLVQLEPGG
                     LHLMLIGLKKPLKDGETIELTLHLKDGKGKTSKIKIEAPVRSGKN"
     gene            complement(2743..3147)
                     /locus_tag="PKW44_07035"
     CDS             complement(2743..3147)
                     /locus_tag="PKW44_07035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR51374.1"
                     /translation="MRRKIWLALLPVMLLLMLWLPPLWLKEQPVEIACPDIVAGCRSP
                     QAGLKVQFDRRPRPMQEFHVLVELSGAREVHASFSMHDMEMGLNRYRLLPDGPARWRA
                     GVMLPACVQGRQDWLMVLEVDGRRYQIPFASD"
     gene            complement(3156..4091)
                     /locus_tag="PKW44_07040"
     CDS             complement(3156..4091)
                     /locus_tag="PKW44_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nudix family hydrolase"
                     /protein_id="HQR51375.1"
                     /translation="MAEQVEAAVAVILREDGQVLLGQRPEGKPWAGWWEFPGGKIEDG
                     ETPFHALQRELHEELGIEAVEAYPWLTRSFAYPERTVKLRFFTVRQWRGEPHGREDQQ
                     VSWQEVANVSVGPLLPANAPVLDALQLPSVYAITNLAELGEQRFFAALERALQQGLRM
                     VQVREPQLDAAGLERFASRVVVACKPYTARILLNGDEAMSRRIGADGIHLSATRLMDL
                     PQRPQMLCAASCHNAAELAQAARLGLDFALLSTVLSTLSHPAAAPLGWAGFSGMVGEY
                     PLPVYALGGLRPGDLRTAWTYGAHGIAMQRAVWEA"
     gene            complement(4084..>4310)
                     /locus_tag="PKW44_07045"
     CDS             complement(4084..>4310)
                     /locus_tag="PKW44_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF815 domain-containing protein"
                     /protein_id="HQR51376.1"
                     /translation="SLSERFGLWLSFYPFDQEAYLDIVRHWLRRFGITRLAPQARQAA
                     LQFALQRGSRSGRVAWQFARDWAGQQGLRG"
BASE COUNT          961 a         1345 c         1218 g          786 t
ORIGIN      
        1 tagtggccag ctacgactac ccattcaacg aacgcatacg cacgctactg cggctggaag
       61 atcttttcgg caagatcctg cattacgctg acggcaagca cgaactcaat caccattgcg
      121 cgttgctggg cattttccag gtgctggacg tcatcgaccg tgccgagctg aaaacggact
      181 tgctgcagga actcgaccgg cagaagatcg tcatggagaa tctgcgcgga aatccggcga
      241 tcgccgagga cgtactcgac gaagttatcg ttgatatcgg agaaaccgcc catgtcatgc
      301 gcaacagcgc cggcaagatc ggccagtcgc tgcgggacaa cgaatggctg atgggcatca
      361 agcagcgcgc cggcattcct ggcggggtgt gcgagttcga cgtaccgtcc taccactact
      421 ggctgggact gccggtggac cagcgccagc agtacctgca aagctggatg gcgccattga
      481 tgccactgca tgacacgctg cgcatcatat tgcatatcct gcgcggcagc ggcacgacca
      541 cgaagcaggt tgcgccgcag ggcagcttcc agcaaatgct gggcggcgcc aaactggcgc
      601 agatgctgcg gattacgatc gacgacgacc tgcactgctt ccccgaagtc agcgccaaca
      661 agtacgtcat caacgtccgc ttcaatacgc tcgaactggc gcaaaagccg cgccagtgcg
      721 acttcgacgt cgacttcaaa ctgacgctgt gtaatctata gtcacgcttc accccacctc
      781 accccacccc acaaacaccc agaaaacccg cataaatact gggttttcat aatttcattg
      841 ttcaccatgc aaaaattact tatttggcgc tcttatggga aaaagtcaaa tacgccgata
      901 caagcgccca aatagttaga attgtgatga gcggccagat caggaggaga tcaactcgga
      961 tattggcctc tggcgtggac atctgaacca gccactccca cccagcgtag cccacccaag
     1021 ccaaagccgc catcaggatc aatctcccac gcggcctgac cacccaccat agcagtaaaa
     1081 gggcaacagc tacgccaagg atatgcgcag gctactgaac gagtagcgat gcaatccagc
     1141 ccatgaagat attcagccgc ccgagatcac cgcatcagga attgtgtagt gctcgtagtc
     1201 attgacttcg accaaccagt cgtagccctg cttcacggcc ttgaccttct cgccaggatt
     1261 ggtggtgacc ttgtcgccca tgaaggtcag cacgcgcttc aggccgcccg gcggagtgat
     1321 atgcacctcg gcattgccgg gttttccgcg gatcacgccg aactcgcact gcactggttt
     1381 ttcattgccc atcctgcacg gcaaaggtcc ggtggcatgg taagcggtgc ccttgacctt
     1441 gacatcagtg gccggggcct tgccgagatc ggaagccttg ggcgtcccag tgatgccgac
     1501 ggtcagcgtg tagttggccg cctcgttgcg tcgtgccgca ctgcgcatca gatacacacg
     1561 caccttgtat tcgccgtcgg cctgcagggt gcccttccat tcgttgccgt cggtcgaacc
     1621 gacgaagatg gcttcatcgt tactaccggg cggcaggacg ttgaagtagt tagccccgtt
     1681 atcagtcttc aacgagacct tcatcgcttg gcccgacttg gcgctcagga tgtagtcgat
     1741 ggtctggtta cccttgatcg cgcctttgac gctcacggaa gaatgaccct tggcaaactg
     1801 gactggtttg gcttgaatcc cttcggcatg cgatatcggg gccaaaacca gcaagaaggg
     1861 agcgattgct gcgattaatt tggcatagag tttcatattt ttttccttag aggtttgcag
     1921 attgaatcca gtctatcatg tggatgtagt cacacctgtg gcacttcatg tccaagcaaa
     1981 tttatgcagc atgctgcata atcccaccgc atcctaacgc actatttttg agtagcccca
     2041 cgagtagcta aaataaaaaa tgctgattaa tcttattgtt tggctttggt tcaagccatt
     2101 attagagctc acgctgtgca acctgtagtg aagctggtcg cgtgccctac ttgcggcgcg
     2161 caaaccgaat acacctcagc caacccttac cgcccgttct gctcccgacg ctgcaagctg
     2221 atcgacctcg gacaatgggc gactgaaaac taccgtatcc cggaaaatca gaagccggaa
     2281 cccgcagacg aggagaaatg acatgaacaa gttctttgca gccgcgctgg ccggcatgtg
     2341 ttttctgatc ggcatggcag cacaggccgg ggacagcctg accgtcagca atgcctgggc
     2401 gcgcgccacc gtgccggggc aggaagtcgg cgccgcttac atggagctcc ggagtgccgc
     2461 cggcgccacc ctgaccggga tggaaagccc cgcgtccgat tccgtcgaaa tccacagcat
     2521 gtcgatgcag ggcgagcaaa tgcaaatgcg catgctggaa gccctgccct tgcccgccgg
     2581 caagttggta caactggaac cgggcggatt gcatctgatg ttgatcggtt tgaaaaaacc
     2641 actgaaagac ggggaaacga tagaactcac gctccacctg aaagacggca agggcaagac
     2701 tagcaagatc aagatcgagg ccccggtcag gagcggcaaa aactagtcgc tggcgaacgg
     2761 tatctggtac cgcctgccat ccacttccag caccatcaac caatcctggc ggccctgcac
     2821 acaggctggc agcatcaccc cagcgcgcca gcgcgccggc ccatcgggca gcaggcggta
     2881 gcggttcaag cccatctcca tgtcgtgcat gctgaaactg gcatgtactt cacgcgcgcc
     2941 ggataattcg accagtacat gaaactcctg catcggccgt ggccggcgat cgaactgaac
     3001 cttgaggccg gcttgcggcg agcgacatcc cgcaacgatg tccgggcagg cgatctcgac
     3061 cggctgttct ttcaaccaca gaggcggcaa ccacagcatc aggagcagca tcaccggcag
     3121 caaggccagc caaatcttgc ggcgcaacgg catcgtcacg cttcccaaac cgcacgctgc
     3181 atggcgatac cgtgtgcgcc gtaagtccat gccgtgcgca agtcgcccgg ccgcaagccg
     3241 cccaaggcat aaaccggcag cggatactcg ccaaccatgc cggaaaaacc tgcccagccc
     3301 agcggcgcgg cagccgggtg gctgagcgtc gacagcacgg tggaaagcag cgcgaaatcc
     3361 agccccagcc tggccgcctg cgccagttcc gcagcattgt gacaggaggc cgcgcacagc
     3421 atttgcgggc gctgcggcaa gtccatcagg cgggtcgcgg aaaggtgtat tccatcggct
     3481 ccaatgcggc gggacatcgc ctcgtcgccg ttgagcagga tgcgcgcggt atagggcttg
     3541 caagccacca ccaccctgga ggcaaagcgc tccagtccgg ccgcgtcgag ctgcggctcg
     3601 cgcacctgca ccatccgcag cccctgttgc aatgcacgtt cgagcgcggc gaagaaacgc
     3661 tgctcgccga gttcggcaag gttggtgatg gcatacaccg aaggcaattg cagcgcatcc
     3721 agaacaggcg cgttagccgg cagcagcggg ccgacgctga cattggccac ctcctgccag
     3781 gaaacctgct gatcctcacg gccgtgcggc tcaccgcgcc attgcctgac agtaaagaag
     3841 cgcagcttga ccgtgcgctc cgggtaggca aagctgcgcg tcagccaggg ataggcctcg
     3901 accgcctcaa tgcccagctc ctcgtgcagc tcacgctgca gggcatggaa tggcgtttca
     3961 ccgtcctcga tcttgccgcc gggaaactcc caccatccgg cccacggctt gccttccggc
     4021 cgctggccga gcagcacctg gccatcttca cgcaggatga ccgcgacggc ggcttcaacc
     4081 tgttcagcca cgcaagccct gctggccagc ccagtcgcgg gcaaactgcc aagccacgcg
     4141 gccgctgcgt gaaccgcgtt gcaaagcgaa ttgcagcgcg gcttgacgtg cctgcggagc
     4201 aaggcgagtg atgccgaagc ggcgcagcca atggcgcacg atgtcgaggt aggcctcctg
     4261 atcgaacggg tagaacgaca gccacaggcc gaagcgctcc gacagcgaga
//
LOCUS       DAOMFA010000135         4254 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_2838_length_4254_cov_5.621100, whole genome shotgun sequence.
ACCESSION   DAOMFA010000135 DAOMFA010000000
VERSION     DAOMFA010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 4254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4254
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2838_length_4254_cov_5.621100"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..518
                     /locus_tag="PKW44_07050"
     CDS             <1..518
                     /locus_tag="PKW44_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HQR51377.1"
                     /translation="AVGPQSVAANGLRRQGVKGALVNIGGNIIAIGKHGERPWRVGIQ
                     HPRRPGPLATLELPDGWAIGTSGDYQRFFELDGQRYCHIIDPRSGRPAQGVQAVTILV
                     PPGPRAGTLSDVASKPLFISGPADWRKAASTLGIAHALLVDDQGGIHLTQAMKQRLNF
                     TEKNVALEELP"
     gene            523..876
                     /locus_tag="PKW44_07055"
     CDS             523..876
                     /locus_tag="PKW44_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019896863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HQR51378.1"
                     /translation="MPGDWLVFCICCVVVAVLFTTLWHNEAAGKLRIRAGDRVFATLS
                     LDQQRTLRVPGPLGISTIVIDRGRVRVASDPGRHQYCVRQGWLSKAGQVAMCLPNQVS
                     LELLGGEKSYDSLNY"
     gene            857..1408
                     /locus_tag="PKW44_07060"
     CDS             857..1408
                     /locus_tag="PKW44_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HQR51379.1"
                     /translation="MTRSTIEITLTPDDHRVARLAAIAIGLHLVESLVPSPLPGVKPG
                     IANIVTLYVLYRFNFATAAWVSLLRVVASSLLLGNFLSPAFALSLVGAISSLTILFFA
                     ARLPRKWYGPVTLSILAAFAHIAGQLLVVRLWLIPHAGVAYLIPVFAAAALFFGLVNG
                     LIAARLLRDNGVVSTDAEPEAST"
     gene            1405..1980
                     /locus_tag="PKW44_07065"
     CDS             1405..1980
                     /locus_tag="PKW44_07065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR51380.1"
                     /translation="MKLIYAPLLLLPLLLCSSVVRAADWVPVAVAGSENQYFYDRSKL
                     NIKDDEISYWQKVVYHAPQNVQGGEAASALLRERIDCAGHTAKRLNYLYYSEAGETLE
                     HVARDESEPVPIIPDTAGDAFEQALCPHVWQKQEELRIKAEQKTADSRPAEVSKQNKK
                     DVADSPGEANAEQAPQKTEPASLPQAMEQLY"
     gene            complement(1987..2598)
                     /locus_tag="PKW44_07070"
     CDS             complement(1987..2598)
                     /locus_tag="PKW44_07070"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HQR51381.1"
                     /translation="MKIPVVSAYSAEQRREMAEAKLDQFFVTVPTELKVRVNSLPARI
                     ARMNARVSVKLQEVLRTTDALFDHAAGFAACAKGCGHCCHVSVPIADFEARYIADSTK
                     IEPVALKHSIRHDLDEFSEKTPCPFLQDGACSIYAVRPLTCRLHVNFDVDNYWCLHEN
                     WKKPEAVIPRPTINALISAYHLLARKTEPVVADIRDFFPSGKQ"
     gene            complement(2595..3620)
                     /gene="trpD"
                     /locus_tag="PKW44_07075"
     CDS             complement(2595..3620)
                     /gene="trpD"
                     /locus_tag="PKW44_07075"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HQR51382.1"
                     /translation="MALTAAQALHKLLARADLTHGEMLEVMHQIMHGEYTSAQMAGVL
                     VALRMKGETVDEIAAAAEVMRALSTKVEVATDEHLVDTCGTGGDGIQTFNVSTVSAFV
                     AAAAGARVAKHGGRSVSSTCGSADVLEALGINVNLTPAQVARCVDDIGIGFMFAPNHH
                     SAMKHAAPVRRELGVRTLFNLLGPLTNPAGAKRQVMGVFDKNLTGKLARVLQQLGSEH
                     VLVVHGADGMDEISFAGDTYVAELKSGEVTEYVLNPQQFGMGLHQAESIRVQNAGESK
                     AMILAVLAGQPGPARDIVLLNAGAAIYVAGVADSLQAGIAKATQVIDNHAASERLKQL
                     VRLSAEL"
     gene            complement(3620..4186)
                     /locus_tag="PKW44_07080"
     CDS             complement(3620..4186)
                     /locus_tag="PKW44_07080"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HQR51383.1"
                     /translation="MLLMLDNYDSFTYNLVQYLGELGQDVRVFRNDEITLEEVAKLKT
                     RHIVISPRPCTPNEACISVPLIHEFAGKVPILGVCLGHQSIGQAFGGKIVHAKQLMHG
                     KTSPVFHHDVGVFRGLPNPFTATRYHSLVIERATCPDCLEITAWTEDGEIMGVRHKTL
                     PVEGVQFHPESILTEYGHELLDNFLKAY"
BASE COUNT          762 a         1279 c         1355 g          858 t
ORIGIN      
        1 aggcggtggg cccgcagagc gtggccgcca acggcctgcg ccgacagggg gtcaaggggg
       61 cgctggtcaa cattggcggc aacatcatcg ccatcggcaa gcacggcgaa cgcccctggc
      121 gcgtcggcat ccagcacccg cgcaggcctg gtccgctggc gacgctggaa ttgccggatg
      181 gctgggcgat cggcacctcg ggcgactacc agcgcttttt cgagctggat ggccagcgct
      241 attgccacat tatcgacccg cgcagcggcc ggccggcaca gggggtgcag gcggtaacca
      301 ttctggtgcc accgggtccc cgcgccggca cgctgtccga cgtcgcttcc aagcccttgt
      361 tcatctccgg gccggccgac tggcgcaagg cggcgtctac cctaggcatc gcccatgcgc
      421 tgctggtcga tgaccagggc ggcatccacc tgacgcaggc gatgaagcag cgcctgaact
      481 tcaccgagaa aaatgtcgct ctggaagaac ttccctaaac ccttgccagg agactggctg
      541 gtattctgca tctgctgcgt ggtggtggcg gtgctgttca ccacgctgtg gcacaacgag
      601 gcggcgggca agttgcgcat ccgcgccggg gatcgcgtgt ttgccaccct gagcctggac
      661 cagcagcgca cgctgcgagt acccgggccg ttgggcatta gtaccatcgt catcgaccgc
      721 ggccgtgtgc gcgtcgccag cgaccccggc cgccaccagt attgtgtcag gcagggctgg
      781 ctatccaagg ccgggcaggt ggcgatgtgt ttgcccaacc aggtgagtct ggaattgctg
      841 ggtggagaga agagctatga ctcgctcaac tattgaaatc acgctcaccc cggacgacca
      901 tcgcgttgcc cgcctggctg ccatcgctat cggcctgcac ctggtggagt cgctggtgcc
      961 ttcgcccttg cccggagtca agccgggcat cgccaatatc gtcacgctgt atgtgctgta
     1021 ccgtttcaac tttgccacgg cagcctgggt ttcgctgctg cgcgtggtgg ccagcagcct
     1081 gctgctgggc aatttcctct cgccggcgtt tgccttgagc ctggtcgggg cgatctccag
     1141 cctgacgatc ctgttctttg cggcacgcct gccacgcaag tggtacggcc cggtgacgct
     1201 cagcatcctg gccgcttttg cccatatcgc cgggcagttg ctggtggtgc gcctgtggct
     1261 gatcccccac gccggcgtgg cttacctgat cccggtattc gccgctgccg cgctgttctt
     1321 cggcctggtc aacgggctta tcgcggcgcg tctgctgcgc gataatggcg tcgtgtctac
     1381 cgatgctgaa ccagaggctt ccacatgaaa ctgatttatg ctcctttgct gctattgccg
     1441 ttgctgctct gcagtagcgt tgtccgcgcc gccgactggg tgccggtggc cgtcgccgga
     1501 tcggaaaacc agtattttta cgatcgcagc aaactcaaca tcaaggacga cgagatcagc
     1561 tactggcaaa aggtggtgta ccacgcgcca caaaatgtgc aagggggcga agccgcctct
     1621 gccttgctcc gggagcgcat cgattgtgcg ggacataccg ccaaacgact caattacctc
     1681 tattactccg aggccggcga gacgctggag catgtggcta gggatgaatc cgaaccggtc
     1741 cccatcattc cggacaccgc cggcgatgct ttcgagcagg cgctttgtcc gcatgtgtgg
     1801 caaaagcagg aagaattgcg catcaaagcg gagcagaaga cagccgacag ccgtccggca
     1861 gaggtgtcaa aacagaataa aaaggatgtg gccgattcgc ccggtgaagc gaatgccgaa
     1921 caggctccgc aaaagacgga accagcctcg ctgccgcagg cgatggagca gttgtattag
     1981 gagtggttac tgtttcccgc tggggaagaa gtcgcggata tccgcgacta ccggttccgt
     2041 tttcctggcc agcaggtggt aggcggaaat cagtgcgttg atggttggtc tggggatgac
     2101 ggcttcgggt ttcttccagt tctcgtgcag acaccagtag ttgtcgacgt cgaagttcac
     2161 atgcaggcgg caggtcagcg gtctgactgc gtagatcgaa caagcgccgt cctgcaggaa
     2221 cgggcacggt gttttctccg agaactcatc aagatcgtgg cggatgctgt gcttcaaggc
     2281 gacgggctcg atcttggtgc tgtctgcgat gtaacgggct tcgaagtcgg cgataggtac
     2341 cgagacgtgg cagcaatggc cgcagccttt ggcgcaggca gcgaaaccgg cagcatggtc
     2401 gaacagggcg tccgtggtgc gcagcacttc ctgtaatttt acggagactc gtgcgttcat
     2461 cctggcgatg cgagcaggca gggaattgac gcgcactttc aactcagtcg gtaccgtgac
     2521 gaaaaactgg tccagcttgg cctcggccat ttcgcgtcgc tgctcagcgg agtatgcgga
     2581 tacgacaggg attttcataa ttcggcggat aatctgacga gctgtttcaa ccgttcgctg
     2641 gcggcgtggt tgtctataac ctgcgttgcc ttggcgatgc ctgcttggag gctatccgca
     2701 acaccggcga cataaatcgc cgcccctgca ttgagcagca cgatatcgcg cgccgggcct
     2761 ggctggccag ccagcacggc taggatcatg gctttggatt ctcccgcatt ctgcacgcgg
     2821 atgctttccg cctggtgcag ccccataccg aattgctgcg gattgagtac gtactcggtg
     2881 acttcgccgc ttttcagttc tgccacgtag gtatcgccgg cgaacgagat ttcgtccatg
     2941 ccgtcggcgc cgtgcactac cagcacatgc tcgctgccga gctgttgcag cacccgtgcc
     3001 agcttgccgg tgaggttttt gtcgaacacg cccatgacct ggcgcttggc tccagccggg
     3061 ttggtcagtg ggccgagcag gttgaacagc gtgcgcacgc caagttcgcg ccgtaccggt
     3121 gcggcgtgct tcatggcgct gtggtggttg ggggcgaaca tgaagccgat gccgatgtcg
     3181 tccacgcacc tggcgacctg cgccggcgtc agattgacgt tgataccgag cgcttccagc
     3241 acgtcggcgc tgccgcaggt cgaagacacc gagcggccgc cgtgcttggc caccctggcg
     3301 ccagcggccg ccgcgacgaa agcgctgacg gtggaaacgt taaaggtctg gatgccgtcg
     3361 cccccggtgc cgcaggtatc gaccagatgc tcatccgttg ccacctcgac cttggtggaa
     3421 agcgcgcgca tcacctcggc ggcggcggcg atctcgtcca cggtctcgcc cttcatgcgt
     3481 agcgcgacca gcacaccggc catctgcgca gacgtgtact cgccgtgcat gatctggtgc
     3541 atcacctcca gcatctcgcc atgggtcagg tcggcgcggg ctagcagctt gtgcagcgcc
     3601 tgtgcagcag tcagcgccat cagtaggcct tgaggaaatt gtccagcagt tcgtggccgt
     3661 attcggtgag gatggattcc gggtggaact gcacgccctc gaccggtagc gtcttgtggc
     3721 gcacgcccat gatttcgcca tcttcggtcc aagcggtgat ttcgaggcaa tctggacagg
     3781 ttgcgcgttc gatcaccaga gaatggtagc gcgtagcagt aaagggattg ggtaggcccc
     3841 tgaaaacgcc gacatcgtgg tggaataccg gcgaggtctt gccgtgcatc agctgtttgg
     3901 catgcacgat tttgccgccg aacgcctggc cgatgctctg gtggccgaga cagacgccaa
     3961 ggatgggcac cttgccggcg aattcgtgga tcagcggcac cgagatacag gcttcgttgg
     4021 gtgtgcacgg gcgcggggaa atcacgatat ggcgcgtttt taacttagcc acttcttcca
     4081 gggtgatctc gtcgttgcgg aacacccgta cgtcctgccc caactcgccc agatactgca
     4141 ccaggttgta ggtgaaggag tcgtaattgt ccagcatcaa cagcatgagg cggcgcccaa
     4201 attacagccc ccgacgcgag aaagtcagcg tggcgttggt gccgccgaac ccga
//
LOCUS       DAOMFA010000136         4249 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_2842_length_4249_cov_5.009299, whole genome shotgun sequence.
ACCESSION   DAOMFA010000136 DAOMFA010000000
VERSION     DAOMFA010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 4249)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4249)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4249
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2842_length_4249_cov_5.009299"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..357
                     /locus_tag="PKW44_07085"
     CDS             <1..357
                     /locus_tag="PKW44_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HQR51384.1"
                     /translation="SKAGSSLGAGSVIVMDESTCMVQTLKRLSYFFFEESCGQCTPCR
                     EGTGWLYRVVKRIVDGQGRMEDLDLLTDVSQNISGRTICALGDAAATPVLSFIKHFRP
                     EFEYYIQHGKSMVTGK"
     gene            357..2741
                     /gene="nuoG"
                     /locus_tag="PKW44_07090"
     CDS             357..2741
                     /gene="nuoG"
                     /locus_tag="PKW44_07090"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HQR51385.1"
                     /translation="MLNIEIDGKPVQVEHGSTIIDAADKIGVAIPRFCYHKKLSVAAN
                     CRMCLVQVEKFAKPLPACATPVSEGMKIFTRSKAAVEAQKSVMEFLLINHPLDCPICD
                     QGGECELQDIAVGYGGVKSRYTEEKRVVLNKNIGPLVSTDMTRCIQCTRCVRFLKEVG
                     GMMELGLINRGEHAEITAYVDKSVQSELSGNIIDLCPVGALTSKPFRYSARSWELVRR
                     PSIAAHDGLGSQIEVQVKNNRVMRVLPREKESINECWISDRDRYSYEGLNSADRLTVP
                     MIKRGGQWVETDWQSALEFAASRIRQVVQKDGGEALGMLVSPNSTMEEGYLAKKIARG
                     LNCGNVDHRLRRSDFRLDGHFQGTPWMGCNIPDLQEMDRVLVIGSNLRNEHPLLTHRL
                     RHAVRMNGAELSLVNPLDDDPLMPVAHKAICAPNDIVNVLSQILKAMSGLQRLSLCLP
                     PSLSQLLEHVQVRDNARAMAESLAGHGKDYDLISPRSAVFLGNIAQHHPRYAEIYSLA
                     EAISALCGATFGALSEAANRVGMDLARVLPRNGALNARTMLENPRQAYLLVNVEPELD
                     CHNAQLANAALRQTGCVVALTAYKSDALRDADVLLPIAPFTETSGTFMSMEGRVQSFA
                     AAVKPLGECRPGWKVLRVLGNLLGLEGFDYDSSEQVKNEIFGGEKPSRVVWNRLNNNL
                     RHLVEVNVRIKSGVLQRVGEIPLTQSDAIVRRAPSLQKAGAASLDFVAMNSVLLARLG
                     LQAGDTARVTQSGASVDLPVRRDDGISDDSVRVPVCAATAALGDLYGEITVEKA"
     gene            2756..3805
                     /gene="nuoH"
                     /locus_tag="PKW44_07095"
     CDS             2756..3805
                     /gene="nuoH"
                     /locus_tag="PKW44_07095"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HQR51386.1"
                     /translation="MHFFQSLWGPLWEPIWGVLAPVWPLLWNLAKIIAIVAPIMGCVA
                     YLTLAERKVIGYMQVRIGPNRVGYYGLLQPIADGLKLLFKEIIIPTAANKFLFLLAPI
                     LVLGPALAAWAVVPFDLDMTLANIDAGLLYILAMTSVGVYGVIIAGWASNSKYAFLGS
                     LRSAAQIVSYEIAMGFALVGVLMAANSLNLGKIVMAQTGGFWQWYWLPLFPLFIVYFI
                     SAVAETNRAPFDVAEGESEIVAGFHVEYSGMAFAVFFLAEYANMILVATLAAIMFLGG
                     WLSPLPFLPDGFPWLAIKVAGLLFLFLWFRATFPRYRYDQIMRLGWKVFIPVTLVWIV
                     VIGAFMQTPLAYLFH"
     gene            3822..>4249
                     /gene="nuoI"
                     /locus_tag="PKW44_07100"
     CDS             3822..>4249
                     /gene="nuoI"
                     /locus_tag="PKW44_07100"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011634014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HQR51387.1"
                     /translation="MNRIKELFQSLMLLELLKGMSLTGRYLFARKITVQYPEERTPMS
                     ARFRGLHALRRYPNGEERCIACKLCEAVCPALAITIESEQRDDGTRRTTRYDIDLTKC
                     IFCGFCEEACPVDAIVETHIFEYHGETRGDLYYTKQMLLA"
BASE COUNT          798 a         1271 c         1329 g          851 t
ORIGIN      
        1 tccaaggccg gttccagcct gggtgctggt tcggtgatcg tcatggacga gtccacctgc
       61 atggtgcaga cactgaagcg cttgtcctat ttcttcttcg aggaatcctg tggccagtgc
      121 acaccctgcc gtgaaggtac cggctggctg tatcgtgtgg tcaagcgtat cgtcgacggc
      181 caggggcgta tggaagacct cgacctgctg accgacgtca gccagaacat ttccggccgc
      241 accatctgcg cgctgggcga cgcagcggcg acgccggtgc tgagtttcat caaacatttc
      301 cggccggagt tcgagtatta catccagcac ggcaagagca tggtgacggg taaataatgc
      361 tgaatatcga aatcgacggc aaaccggtac aggtcgaaca cggcagcacc atcattgatg
      421 cggccgacaa gatcggcgtg gcgatcccgc gtttctgcta ccacaagaaa ctgtcggtgg
      481 cggctaactg ccgcatgtgt ctggtgcagg tggaaaaatt cgccaagcct ttgccagcct
      541 gcgccacacc ggtttccgaa ggcatgaaga tttttacccg ctccaaggct gccgtcgaag
      601 cacagaagag cgtgatggag ttcctgctga tcaaccatcc gctcgattgc ccgatctgcg
      661 atcagggggg ggaatgcgaa ctgcaggata tcgctgtcgg ctatggcggc gtcaagtcac
      721 gctacaccga agagaagcgc gtggtgctga acaagaacat cgggcctttg gtttccaccg
      781 acatgacgcg ctgcatccag tgcacgcgtt gcgtgagatt cctgaaggaa gtcggcggca
      841 tgatggaact gggcctgatc aaccgtggcg agcatgccga gatcaccgct tatgttgaca
      901 agagcgtgca atccgaactg tccggcaaca tcatcgacct gtgcccggtg ggcgcgctga
      961 ccagcaagcc gttccgctac tcggcgcgca gctgggaact ggtgcgccgt ccgtccatcg
     1021 ccgcccacga cggcctgggc tcgcagatcg aggtacaggt caagaacaac cgcgtcatgc
     1081 gtgtgctgcc gcgcgagaag gaaagcatca acgagtgctg gatttccgac cgcgatcgct
     1141 attcctacga aggcctcaac agcgccgatc gtctgaccgt gcccatgatc aaacgcggcg
     1201 ggcagtgggt ggaaaccgat tggcagagcg cgctggaatt cgccgctagc cgtatccggc
     1261 aggtcgtgca gaaagacggc ggcgaggcct taggcatgct ggtatcgccc aacagcacca
     1321 tggaagaggg ctacctggcc aagaaaatcg cgcgcggcct gaactgcggc aacgtggatc
     1381 accgtctgcg tcgtagtgat tttcgtctgg acggtcattt tcagggcacg ccctggatgg
     1441 gctgcaacat cccggatctc caggagatgg accgcgtatt ggtgattggc agcaatcttc
     1501 gcaacgagca tccgttgctg acacaccgcc tgcgtcacgc ggtcaggatg aatggcgcgg
     1561 aactgtcact ggtcaatccg ctggacgatg atccgctgat gccggtggca cacaaggcga
     1621 tttgtgcgcc gaatgatatt gttaatgtgc tgtcgcaaat cctcaaggcc atgtccggcc
     1681 tgcagcgcct gtcactgtgc ctgccgcctt cgctcagtca actgctggag catgtgcagg
     1741 tgcgtgacaa tgcccgggcg atggcggaaa gcctggccgg ccacggcaag gattacgacc
     1801 tgatcagccc gcgctctgcg gtgttcctcg gcaacatcgc ccagcatcat ccgcgctacg
     1861 cggaaatcta cagtctggcg gaagccatct ccgcgctgtg cggcgccacc ttcggcgcat
     1921 tgtccgaggc tgccaaccgc gtcggcatgg acttggcgcg tgttttgccg cgtaatggcg
     1981 cgctgaatgc ccgcaccatg ctggaaaatc cgcgccaggc ctaccttctc gtcaacgtcg
     2041 agccggaact ggactgtcac aatgcgcaac tcgccaatgc tgcactcagg cagaccggtt
     2101 gcgtggtagc gctgaccgcc tacaagtcgg acgccttgcg cgatgccgac gtgctgttgc
     2161 cgatcgcgcc gttcaccgaa acctccggca cgttcatgag catggaaggg cgggtacaga
     2221 gcttcgccgc cgcggtaaag ccgctgggtg aatgccgtcc ggggtggaaa gtgctgcgcg
     2281 tgctgggcaa tctgctcggc ctggaaggct tcgattacga cagcagcgag caggtgaaga
     2341 atgaaatctt cggcggcgag aagccgtcca gagtggtgtg gaatcgcctc aacaacaacc
     2401 tgcgccacct ggtcgaggtc aatgtgcgga tcaagtcggg cgtgctgcag cgcgtcggcg
     2461 aaatcccgct gacccagtcg gatgccatcg tgcgtcgcgc gccgtccctg caaaaagccg
     2521 gcgcagcttc gctggacttt gttgccatga acagcgtgct gctggccaga ttgggcttgc
     2581 aggctggcga tacggcgcgg gtaacgcaat ccggcgccag cgtggatctg ccggtgcgcc
     2641 gcgatgacgg cattagcgac gacagcgtgc gggtgccggt atgcgctgcc acggcggcac
     2701 tgggcgatct ttacggcgaa attacggtgg agaaagcgta atggaagccc acgtcctgca
     2761 tttcttccag tcgttgtggg gccccttgtg ggagccgatc tggggcgtgt tggcgccggt
     2821 ctggccgctg ctgtggaacc tggccaagat catcgccatc gttgccccca tcatgggctg
     2881 cgtggcttac ctgacgctgg ccgagcgcaa ggtgatcggt tacatgcagg tgcgcatcgg
     2941 gccgaaccgc gtgggctatt acggcctgct gcaacccatt gccgacggcc tcaagctgct
     3001 gttcaaggaa attatcattc cgacggcggc caacaagttc cttttcctgt tggcgcccat
     3061 cctggttctg gggccggcgc tggcggcgtg ggcggtggtg ccgttcgacc tggacatgac
     3121 cctggccaac attgacgctg ggctgctgta catcctggcc atgacctcgg tcggcgtata
     3181 cggcgtcatc atcgccggct gggcctccaa ttccaagtac gccttcctcg gttcgctgcg
     3241 cagcgcggcg cagatcgtct cctacgaaat cgccatgggg ttcgccctgg tgggcgtgct
     3301 gatggcagcc aatagcctca acctgggcaa gatcgtgatg gcgcagacgg gtggcttctg
     3361 gcagtggtac tggctgccgc tgttcccgct gttcatagtg tatttcatca gcgccgtagc
     3421 cgaaaccaac cgtgcgccgt tcgacgtggc ggaaggcgaa tccgaaatcg tcgccggttt
     3481 ccacgtggaa tattccggca tggcctttgc cgtgttcttt ctcgccgagt acgccaacat
     3541 gatcctggtc gcgaccttgg ccgccatcat gttcctcggc ggctggctgt cgccgctgcc
     3601 gttcttgccg gatggcttcc catggctggc gatcaaggtg gcgggactgt tgttcctgtt
     3661 cctgtggttc cgcgccacct tcccgcgcta ccgctacgac cagatcatgc gtctgggttg
     3721 gaaggtgttc atcccggtga cgctggtgtg gatcgtggtg atcggcgcct tcatgcaaac
     3781 cccgctggct tacttgttcc attgaggggg tgcgagaaat gattaaccgc atcaaagaac
     3841 tgttccagag cctgatgctg ctcgaactgc tcaagggcat gtcgctcact gggcgctacc
     3901 tgttcgcgcg caagatcacc gtgcagtacc cggaagagcg cacgccgatg tcggcacgct
     3961 tccgcggcct gcatgcgttg cgccgctacc ccaatggcga ggagcgctgc attgcctgca
     4021 agctgtgcga ggcggtatgc ccggcgctgg cgattaccat cgagtccgag cagcgtgacg
     4081 acggtaccag aagaaccaca cgctacgaca tcgacctgac caagtgcatc ttctgcggct
     4141 tctgcgagga ggcctgcccg gtcgatgcca tcgttgaaac gcacatcttc gaataccacg
     4201 gcgaaacccg tggcgacctt tactacacca agcagatgct gctggccaa
//
LOCUS       DAOMFA010000137         4231 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_2863_length_4231_cov_5.288793, whole genome shotgun sequence.
ACCESSION   DAOMFA010000137 DAOMFA010000000
VERSION     DAOMFA010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 4231)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4231)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4231
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2863_length_4231_cov_5.288793"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..245)
                     /locus_tag="PKW44_07105"
     CDS             complement(<1..245)
                     /locus_tag="PKW44_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018507954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HQR51388.1"
                     /translation="MSLLLFSSCLMAQELYAGRVLTGGLFLYYVKAGDSLTSVGARFG
                     METKLLAQMNDLPAYASLKPGQTLEVDNRHIAPAVSD"
     gene            complement(292..1749)
                     /locus_tag="PKW44_07110"
     CDS             complement(292..1749)
                     /locus_tag="PKW44_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase family protein"
                     /protein_id="HQR51389.1"
                     /translation="MLATLKQYAAVADETTLEQPTHVLFVLPETRLAQLPYGPVLDAK
                     LKRLRAKYADLGKNPVNADLPCGGAASWVVLKAAMSPFERHDALRKALAPLLEEHPEK
                     LSIAVFGESEQRAIAAHDAIYCAWVNGTPLPTRKRKSEARSLKKVTLYGHAEADGYAA
                     LRARVEGNTLTRQLTVLPPNDLTPAIYRDKVKALAVEHGWVHEEYDLKRLRKLGAGAF
                     VAVAQGSDPEDAAIVHLSCAPKKAKKTIALVGKGICFDTGGHNLKPARYMHGMHEDMN
                     GSAVALGILLAATLARLPVKIDCWLAIAQNHISPRAYKQNDVVTALNGTTIEIIHTDA
                     EGRMVLADTLTLASRAKPDVMLDFATLTGSMATALGDRYSGILGNRPELLCKAVGAGR
                     AAGERLCAFPFDADYDTDLDSNIADIKQCTLDGGADHILAARFLSRFIEHDVPWLHVD
                     LSSSNRKGGLGAVASDVNGFGVGLGLELIRSLVTE"
     gene            1866..2807
                     /locus_tag="PKW44_07115"
     CDS             1866..2807
                     /locus_tag="PKW44_07115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HQR51390.1"
                     /translation="MLRSLWQCDGITHSGAVAGLLALLLTAAQASAGEIEPRSYVNTP
                     VGINFAIVGYAHTEGGLSTAASSPLQDAELKIDTEVLAFARSLDVGGNSGKFDVIVPY
                     SQLSGTAMVAGQPRERNVSGLNDPRFRFSVNFYGSPALSLQEFASYQQDVLIGASVQV
                     SAPLGQYDSSRLVNLGTNRWSIKPDIGISKALGALTLELSAGVTFFTDNNDFFGGQKL
                     EQDPVYSTQLHANYNFGNGVWAAISGNYDYGGRTTVDGVRKDDVQSNSRIGATLALPV
                     NRNNSIKIFASSGVSTRTGSDYDLFGAAWQYRWGGGL"
     gene            2899..3735
                     /gene="serB"
                     /locus_tag="PKW44_07120"
     CDS             2899..3735
                     /gene="serB"
                     /locus_tag="PKW44_07120"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HQR51391.1"
                     /translation="MRLVIQGPTLGSLQLQKIAGLCGSSQFTELESNAYSLRGDFPGQ
                     SEIKAYCSAQGIDCAFVPDGQVMENFGLVVMDMDSTLISIECIDEIADMQGLKPQVAA
                     ITESAMRGEIEFAESLRRRVALLQGLDESALQRVYDERLQLNPGAQKLIDTLKERTIK
                     TLLVSGGFNYFTDGLKQRLGLDYALGNRLEIVDGKLTGQALGDIIDAQGKADWLARTR
                     DALGLRKEQVIAIGDGANDLKMMAAAGVGIAYHAKPVVREQATYALNHVGLDGVLNLF
                     RS"
     gene            complement(3741..>4231)
                     /locus_tag="PKW44_07125"
     CDS             complement(3741..>4231)
                     /locus_tag="PKW44_07125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="gluconolaconase"
                     /protein_id="HQR51392.1"
                     /translation="LNDLEADGLGNLYVSDSGDLKGKGGAVYQVSKSGKVRLIASGDK
                     DARIQAPNGLLLGGRGTLLEVDFDSGILYRIKIKSGEMEKVAEGFGGGDGIVRAANGT
                     LYVSDWKGGRVFAVSKKGEVTEFHGGFMAAADIGLSHDGKYLLVPDMKAGELVWLPLE
                     SK"
BASE COUNT          837 a         1320 c         1243 g          831 t
ORIGIN      
        1 tcatcactca ccgcaggggc gatatggcgg ttatctactt ccagagtctg gccaggtttg
       61 agcgatgcat acgccggcaa atcgttcatc tgcgccagca gtttcgtctc catgccgaaa
      121 cgcgcgccaa cgctggtcag gctgtcaccc gcctttacgt aatagagaaa cagccccccg
      181 gtcagaaccc gcccggcata aagctcctgc gccatcaggc aggaagagaa cagcagcagg
      241 gacaaacacc ctatttgcct gatggccaga atcagcgcgg gattcagcat gctattcagt
      301 gaccagactc cgtatcagtt ctagccccaa ccccaccccg aaaccgttga catcgctggc
      361 taccgccccc aggccgccct tgcggttgct ggaggagaga tcgacatgca gccaggggac
      421 atcatgctcg ataaatctgg acaggaagcg cgcggcgagg atatggtcgg cgcccccatc
      481 cagcgtgcat tgcttgatat cggcgatgtt gctgtcaagg tcggtatcgt aatccgcgtc
      541 gaagggaaag gcgcacaacc gctcgcccgc tgcccggcca gcgccgaccg ccttgcacag
      601 cagttccgga cgattgccga ggatgccgct gtagcggtca cccagtgctg tcgccatgct
      661 gccggtaagg gtggcaaaat ccagcatgac atcgggcttg gcacgcgaag ccagggtgag
      721 cgtatcggct agcaccatgc ggccctcggc gtcggtgtgg atgatctcga tggtggtgcc
      781 gttgagcgcc gtcaccacgt cgttctgctt gtaggccctg gggctgatat ggttctgcgc
      841 gatggccagc cagcagtcga tcttcaccgg caatctggcc aacgttgctg ccagcaggat
      901 gccaagcgcc acggcagagc cgttcatgtc ctcgtgcatg ccgtgcatgt aacgcgccgg
      961 tttgagatta tggccgccgg tgtcgaagca gatgcccttg cccaccaggg caatggtttt
     1021 cttcgctttc ttcggtgcgc agctcagatg cacgatggca gcatcttccg ggtcggatcc
     1081 ttgcgccacg gcgacaaaag caccagcgcc cagcttgcgc agccgcttca gatcatattc
     1141 ctcatgcacc cacccatgtt cgaccgccag cgccttgacc ttgtcacggt agatagcggg
     1201 cgtcaggtcg ttgggtggca acacggtgag ttggcgcgtc agcgtgttgc cttcgacgcg
     1261 ggcccgcagc gcggcatagc catccgcctc ggcatggccg tataacgtca ccttcttcag
     1321 cgaccgcgcc tcgctcttgc gctttcgggt gggcagaggc gtcccattga cccaggcgca
     1381 gtagatcgcg tcatgtgcag cgatggcacg ctgctcactc tcgccgaaca ccgcgatcga
     1441 caacttctca ggatgttcct ccagcagcgg cgccaacgcc ttgcgcaacg cgtcatgacg
     1501 ctcaaaaggc gacattgccg ccttcagcac cacccaggag gccgctccgc cgcaaggcag
     1561 atcggcgttg accgggttct tgcccaagtc cgcatacttg gcacgcaagc gcttgagttt
     1621 ggcgtccagc accgggccgt atggcagttg cgcaaggcgc gtctcgggca gcacgaacag
     1681 cacatgcgtc ggctgttcca gcgttgtctc gtccgccact gcggcgtatt gcttcagggt
     1741 tgcaagcatg gttttctgta cctttgatga tttacaattc gattctctca cagtcaccgc
     1801 aaagtcttgc aacaagcaga ttcccgatca tgccaaagga tgccaccact aaagtatgct
     1861 gcgtaatgct gcgtagcctc tggcaatgtg acgggattac acattcaggc gcggtggcag
     1921 gcttgctggc gctgctgctc acggcggcgc aggcaagcgc cggggagatc gagccgagat
     1981 cttacgtcaa cacgccagtc gggatcaatt tcgcaatcgt gggctatgcc cataccgaag
     2041 gcggcctgtc gactgcagca tcgtcgccgc tccaggacgc agaactcaag atcgataccg
     2101 aggtgctggc ttttgcgcgc tcgctggatg taggcggcaa ttcgggaaag ttcgatgtca
     2161 tcgtgcccta ctcccagtta tcgggaaccg cgatggttgc agggcaaccg cgagagcgca
     2221 acgtttccgg gttgaacgat ccgcgcttcc gtttctcggt caatttctac ggctcccccg
     2281 cactgtcgct gcaagagttc gccagctacc agcaggatgt actcatcggg gcaagtgtcc
     2341 aggtatccgc cccgctcggc cagtacgact ccagccggtt ggtcaatctc ggcaccaacc
     2401 gctggtccat caaacccgac atcggcatct ccaaggcgct gggcgcgctg acgctggagc
     2461 tttccgccgg cgtgactttc ttcaccgaca acaacgactt cttcggtggt cagaagctgg
     2521 agcaggaccc ggtctattcg acgcaattgc acgccaacta taactttggc aacggcgtgt
     2581 gggctgcaat aagcggaaac tatgattacg gcgggcgtac caccgtagac ggcgtgcgga
     2641 aagacgatgt gcagagcaac tcgcggattg gcgccaccct ggcattaccg gtcaaccgga
     2701 acaactcgat caagattttc gccagcagcg gagtatccac ccgcaccggc agcgattacg
     2761 acctgttcgg cgctgcctgg cagtaccgct gggggggcgg attgtaaaga attccgacat
     2821 ccgccaccat gacggcacgc cagtcaggcg ggtcggccat gtataatcgc ggcttcacgt
     2881 aaaaccaacc atacggtcat gcgccttgtc attcagggcc ccacgctggg atccctccaa
     2941 ctgcagaaaa ttgccggatt atgcggatcc agccagttca ccgaacttga atccaatgcc
     3001 tatagcctgc gcggcgattt ccccggccag tccgagatca aggcttactg ttcggcgcag
     3061 ggcatagact gcgccttcgt tcctgacggg caggtaatgg aaaacttcgg cctggtagtg
     3121 atggacatgg attcgaccct gatttctatc gagtgcatcg acgagatcgc cgacatgcag
     3181 ggcttgaagc cgcaagtagc tgccattacc gagtcggcca tgcgtggcga gatcgagttt
     3241 gccgagagcc tgcgccgacg cgtagcgcta ctgcaagggt tggatgaatc ggcgctgcag
     3301 cgggtctacg acgagcgcct gcagctcaac cccggcgcac aaaagctgat cgacacgctc
     3361 aaggaaagaa ccatcaagac gctgctggtt tcaggcggct tcaactattt taccgacggg
     3421 ctgaaacagc gcctgggttt ggactacgcc cttggcaaca gactggagat tgtcgacggc
     3481 aagctcaccg gccaagcgct gggcgacatc atcgacgccc aaggcaaggc cgactggcta
     3541 gcgaggacac gcgacgcgct gggattgcgc aaggaacagg tgatcgccat cggcgacggc
     3601 gccaacgacc tcaagatgat ggcggcggcg ggggtgggca tcgcctacca cgccaagccg
     3661 gtagtccgcg agcaggccac ctacgccctc aaccatgttg ggctggacgg ggtgttgaac
     3721 ctgttccgca gttaagccgt ctatttgctc tccaggggta gccagaccag ttcacccgcc
     3781 ttcatgtcgg gcaccagcag gtacttgccg tcatggctca gtccaatatc ggcggcggcc
     3841 atgaagcccc catgaaattc cgtgacttcg ccttttttgc tgacggcgaa cacgcgcccg
     3901 cctttccagt cgctgacgta aagcgtgcca ttggcggcgc gcacgatgcc atcaccgccg
     3961 ccgaagcctt cggcgacttt ctccatttcg ccactcttga tcttgatgcg gtagaggatg
     4021 ccggagtcga aatcgacttc cagcagggtg cctctgccgc ccagcagcaa gccgttgggg
     4081 gcctggatgc gcgcatcctt gtcaccgctg gcaatcagcc tgaccttgcc gctcttgctc
     4141 acctggtaca cggcgccgcc cttgcctttc aggtcgccgc tgtcggaaac gtagaggttg
     4201 cccaggccgt cggcctccag gtcgttgagg a
//
LOCUS       DAOMFA010000138         4143 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_2951_length_4143_cov_5.947407, whole genome shotgun sequence.
ACCESSION   DAOMFA010000138 DAOMFA010000000
VERSION     DAOMFA010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 4143)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4143)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4143
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2951_length_4143_cov_5.947407"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1520)
                     /locus_tag="PKW44_07130"
     CDS             complement(<1..1520)
                     /locus_tag="PKW44_07130"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012777554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HQR51393.1"
                     /translation="MSDLTAIKGVTKPLLAKLAKLGIATLDDLLLHLPLRYQDATRLT
                     PVRDLRVGDQAQVEGEIVHAETQYRPRKALICQLQDDAGGLLHLRFLHFYPGQVAMLK
                     PGVRIRAFGEPRPGFFGAEMVHPQCRAVKEGMPLDEALTPVYPTTSGLSQATLRKLVA
                     AAMQQTDLGETLPADVYCALALPNFADSLNLLHHPPSDAALETLQGRSHAAWRRLAFD
                     ELLAQQLSMRWHHARRRDQGAPRLQPQQRLTTALLDSLPFTLTDAQQKVWTEINRDLG
                     QPHPMQRLLQGDVGSGKTVISALAALQAVENGWQAAIMAPTEILAEQHYRKLHDWLTP
                     LGINLAWLTGTQPKSARATALAAVASGTAQVVVGTHALFQELVQFARLGLAVVDEQHR
                     FGVHQRLALRQKGVQPHQLMMSATPIPRTLAMSYYADLDVSVIDQLPPGRTPVTTKLV
                     SESRREEVLTRVREACWQRRQAYWVCPLIEESEALQLKTALETYEKVTAALPEMKVG"
     gene            complement(1541..1924)
                     /locus_tag="PKW44_07135"
     CDS             complement(1541..1924)
                     /locus_tag="PKW44_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HQR51394.1"
                     /translation="MARQIISTDGAPKAIGTYSQAVKVGDTIYLSGQIGLDPASMQLM
                     EGIEAQVRRVFDNLSAVAAASGGSLADIAKLNIFLTDLGHFALVNSIMAEYFKEPYPA
                     RAAVGVASLPRGALVEADAVMVLGA"
     gene            complement(1935..4088)
                     /locus_tag="PKW44_07140"
     CDS             complement(1935..4088)
                     /locus_tag="PKW44_07140"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HQR51395.1"
                     /translation="MLPADSEDSQLSLRLKQYLKPEDIGQVMEAYRFSAAAHEGQLRK
                     SGDPYISHPVTVAGILAELHLDPPSLMAALLHDVMEDTEVTKQQLAEQFGKTVAELVD
                     GVSKLDKIEFQTATHAQAENFRKMLLAMSQDVRVILVKLADRLHNMRTLDVMAPEKRR
                     RIGRETLEIYAPIANRLGLNSIYQELEDLSFKNLYPMRFQVLNKAVKAARGNRKEVVG
                     KILEAIKHKLADAQIEAEVSGREKHLYSIYKKMAEKTGTFSQIFDIYGFRIVVGDTPT
                     CYLALGALHGLYKPIPGKFKDYIAIPKANGYQSLHTTLFGPFGTPIEVQIRSREMHNI
                     ADAGVAAHWLYKTSGADLSALQQQTHQWLQRLLEIQSESVDSVEFLEHFKVDLFPDEV
                     YVFTPKGKIMALPRGATAVDFAYAVHTDVGNRCVAVKINHELAPLRTELRNGDHVEII
                     TAAHSKPNPVWLNYVATGKARAHIRHFLKTMKSEESAVLGERMLNQALRALHADPGQI
                     DKVRWDKLVRDYGVRDREEILTDIGLGKRLNVMVARQLLAMAEAHGERSGKQLGSITI
                     RGTEGMAVQFAPCCRPIPGDPILGFINKGKGLVVHTHDCPAIRKFRMDPDKWLDVEWD
                     PDTRRLFKVNLKLAVANQPGVLAKIAAGIAAAGSNIDNVGMEESDDSAYTNMNFTVQV
                     EDREHLARLMRSLRKIPEVARINRVKGSANDHKHQ"
BASE COUNT          717 a         1230 c         1331 g          865 t
ORIGIN      
        1 ccgactttca tttctggcag ggctgcggtt actttttcgt aggtttccag cgcggtcttg
       61 agttgcagcg cttcgctttc ctcgattaac gggcacaccc agtaagcttg ccgcctttgc
      121 cagcaggcct cgcgtacgcg agtgagtact tcttcacggc gcgattcgga tacaagtttt
      181 gtggtaaccg gtgtacggcc aggcggcagc tggtcgatca ccgacacgtc gaggtcggcg
      241 taatagctca tcgccaacgt gcgcggaatg ggggtggcgc tcatcatcaa ctgatgtggc
      301 tgtacgcctt tttgccgtaa cgccaagcgc tgatgcacgc cgaaacggtg ctgttcgtcg
      361 accaccgcca gccccagtcg ggcaaactgc actagttcct ggaatagggc atgggtgcca
      421 accacaactt gcgccgtgcc gctggcaacc gctgccagtg cggttgcgcg tgctgacttg
      481 ggctgagtgc cggtgagcca ggcaaggttg atgcccagcg gcgtcaacca gtcgtgcagt
      541 ttgcggtaat gttgttcggc cagaatttcg gtgggtgcca taatggcggc ctgccagccg
      601 ttttccaccg cctgcagcgc ggccaatgcc gagatgaccg tcttgccgct gccgacgtcg
      661 ccttgcagca ggcgttgcat cggatgcggc tgaccaaggt cgcggttgat ttccgtccat
      721 actttttgct gggcatcggt gagggtgaac ggcaggctat ccagcagggc cgttgtcagg
      781 cgctgctgag gctgcagcct gggcgcaccc tgatcgcggc ggcgcgcatg atgccagcgc
      841 atggagagct gctgggccag caactcgtcg aacgccagcc gccgccaggc agcgtggctg
      901 cgcccttgca aagtttccag cgcggcatcg gatggtggat ggtgcagaag gttcaggctg
      961 tcggcaaaat ttggcaacgc cagcgcgcag taaacatcgg ccggcaacgt ttcgccgaga
     1021 tcggtctgtt gcatggccgc agcgacaagc ttgcgcaagg tggcctgcga cagtccggag
     1081 gtggtcgggt ataccggcgt caaggcctca tcgagcggca tcccctcctt caccgcccgg
     1141 cattgcggat gcaccatctc cgcgccgaaa aatcccggcc gcggttcgcc aaaggcgcgg
     1201 atgcgcacgc ccggcttgag catggccacc tggccaggat aaaaatgcag gaagcgcagg
     1261 tgcagcaagc caccggcgtc atcctgcagc tgacagatca gcgccttgcg cggacggtac
     1321 tgggtttctg catggacgat ctcgccctcc acctgcgcct gatcccccac ccgcaggtcg
     1381 cggacagggg tgaggcgggt ggcgtcctgg tagcgcagcg gcaggtgcag cagcaggtcg
     1441 tctagcgtgg cgatgccgag cttggccagt tttgccagca agggcttggt gacgcccttg
     1501 atcgcggtca ggtcggacac gacggcgggg cgcggcgaat tcaggcgccc agcaccatca
     1561 ccgcatcggc ttccaccagt gcgcctctgg gcagggaagc tacgccaaca gcggcgcgtg
     1621 ccgggtaggg ttccttgaaa tactcagcca tgatgctgtt aaccagagcg aagtgaccga
     1681 ggtcggtgag aaagatattg agcttggcga tgtcggccag cgagccgccg gaggctgcgg
     1741 ccacagcgga gagattgtcg aacacacgcc gcacctgcgc ttcgatgcct tccatcaatt
     1801 gcatgctggc ggggtcgagg ccgatctgtc ctgacaggta gatggtgtcg ccgaccttca
     1861 cggcctggga gtatgtgccg atagccttgg gagcgccgtc ggtgctgatg atttgtcttg
     1921 ccatgctgcc ttccttattg gtgtttatgg tcgttggcgc tgcccttgac ccggttgatg
     1981 cgggccactt ccggaatctt gcgcaggctg cgcatgaggc gcgcgaggtg ttcgcggtct
     2041 tccacctgca cggtgaaatt catgttggta taggcactgt cgtcactttc ttccatgccg
     2101 acattgtcga tgttggagcc ggcagcggcg atgccggcgg caatcttggc cagaacgccg
     2161 ggctggttgg ccaccgccag cttgagattg accttgaaca ggcggcgcgt atccgggtcc
     2221 cattccacgt ccagccactt gtccggatcc atgcggaatt tacggatggc gggacagtcg
     2281 tgggtatgga ccaccagccc cttgcccttg ttgataaagc ccagaatggg gtcgccgggg
     2341 atcggccggc agcaaggcgc gaactgcacc gccatgccct cggtgccgcg tatggtaatg
     2401 ctgccaagct gcttgccgct gcgctcgcca tgcgcttccg ccatcgccag cagctgtcgc
     2461 gccaccatca cgttgaggcg cttgccgaga ccgatgtcgg tgaggatttc ctccctgtcc
     2521 ctgacgccat agtcgcgcac cagcttgtcc catcgcacct tgtcgatctg cccggggtcg
     2581 gcatgcaggg cgcgcaaagc ctggttgagc atgcgctcgc ccagcaccgc cgactcctca
     2641 gacttcatgg tcttgaggaa atgccggata tgggcgcgcg ccttgccggt ggcgacgtaa
     2701 ttgagccaca ccgggttggg tttggaatgg gcggcggtga tgatttccac gtggtcgccg
     2761 ttgcgcagct cggtcctgag cggcgccagc tcatggttga tcttgaccgc cacgcagcgg
     2821 ttgccgacat cggtgtgcac ggcataggcg aaatccactg ctgtcgcccc gcgcggcagc
     2881 gccatgatct tgcccttggg ggtgaacacg tagacttcat ccgggaacag gtcgaccttg
     2941 aaatgttcca ggaattccac cgaatcgaca ctctccgatt gaatttccag caggcgttgc
     3001 agccactggt gggtctgctg ctgcaaggcc gaaagatcgg caccgctggt cttgtacagc
     3061 cagtgcgcgg ccacgccggc atcggcaatg ttgtgcattt cgcggctgcg gatctgcact
     3121 tcgatggggg tgccgaacgg gccgaacagg gtagtatgca gggactggta gccgttggcc
     3181 ttgggaatcg cgatgtaatc cttgaacttg ccggggatgg gcttgtaaag cccatgcagc
     3241 gcgcctagcg ccaggtagca ggtcggggtg tctccgacga cgatgcggaa accataaata
     3301 tcgaaaatct gcgagaaggt gccggttttt tccgccattt tcttatagat gctgtacagg
     3361 tgcttttcgc ggccgctgac ctcggcctcg atttgcgcat ccgccaactt gtgcttgatg
     3421 gcctccagaa tcttgccgac cacttccttg cggttgccgc gcgccgcctt caccgccttg
     3481 ttgagcacct ggaagcgcat cgggtaaagg ttcttgaagc tgagatcctc gagttcctgg
     3541 tagatggagt tgagtcccag ccggttagca atcggggcgt agatttccag cgtctcgcgg
     3601 ccgatgcggc gacgcttttc cggcgccatc acatccaggg tacgcatgtt gtgcaggcgg
     3661 tcggccagct tgaccaggat gacccgaaca tcctgcgaca tggccagcaa cattttgcgg
     3721 aaattttccg cctgcgcgtg tgtcgcagtc tggaactcga tcttgtccag cttgctgacg
     3781 ccatccacca gttccgccac tgtcttgccg aattgttcgg ccagttgctg cttggtgact
     3841 tcggtatcct ccatcacgtc atgcagcaag gccgccatca ggctaggcgg atcaagatgc
     3901 agctcggcca ggatgccggc taccgtaaca gggtgggaaa tatagggatc tccgctcttg
     3961 cgcagctgac cttcatgtgc agcggcactg aagcggtaag cttccatcac ctgcccgata
     4021 tcttcgggct tcaggtactg cttgagacgc agactgagct gcgagtcctc gctgtcggct
     4081 ggcagcaagg gagggggggg cggaaccgca tgggcggcgg agccgaggcg gtggtgtacc
     4141 tgc
//
LOCUS       DAOMFA010000139         4073 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_3023_length_4073_cov_4.358885, whole genome shotgun sequence.
ACCESSION   DAOMFA010000139 DAOMFA010000000
VERSION     DAOMFA010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 4073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4073
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3023_length_4073_cov_4.358885"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..690
                     /locus_tag="PKW44_07145"
     CDS             <1..690
                     /locus_tag="PKW44_07145"
                     /inference="COORDINATES: protein motif:HMM:NF014066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HQR51396.1"
                     /translation="FSPAPLKPIADSEIFMDEVLPAGDHSVYFMPEGKLSSLLSEQEH
                     EQVMQLADFHVMDRAAVNRMKPWLLAVMFDQPRPASIFSQDIMLRGIAQNHAKPLLAL
                     EENDRHFLALDSLSMEEQLTMLRTVLKRSQEDKERDFESLLSAYLSGNLAQIAGVDEE
                     MTGGLLPADIWQKIRVKLLDERNMEMADRIIQQAGTSTVFVAVGASHLAGSGGLLQRL
                     RSAGFRLSPVK"
     gene            700..1665
                     /locus_tag="PKW44_07150"
     CDS             700..1665
                     /locus_tag="PKW44_07150"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HQR51397.1"
                     /translation="MRAHSFSFVRILFLALLLHTAVTRHSHADDSFYDLPPPGQRVDI
                     GGYKLHIHCQGTGSPTVILDAGLGDWSSHWASVQALLKGDTQVCSYDRAGYGWSDPGP
                     RPRASAQLATELHALLKKAEIPPPYVLVGHSFGGFNMRLFSTTFDDEVVGLVLVDASH
                     AGSLPYRRDENGSGSNPAASNYRVLMVAAQADILKLPPEAIPAVHNDFLHTKSIATAR
                     SEYRALADSVSQVQQAPGLGDIPLIVISRGIHEWGESAEGAAREASWQAQQDELVRLS
                     RRGTHKIASRSGHHIHLDEPGLVADAIRELVVKAREEQVPTGGQP"
     gene            1783..3597
                     /locus_tag="PKW44_07155"
     CDS             1783..3597
                     /locus_tag="PKW44_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HQR51398.1"
                     /translation="MKSRQTTLIPPETAVTLDGLFHERARRFPNSTAYQYFDHHLCDW
                     RELTWGQVLQDIARWQAALIHEGFQPGDRVAIMLKNCPQWVIFDLAALGLGLVTVPLY
                     VSDRPENVAHVLQDSGARLLLIDSAEHWHPLHEACSQISTLTRVVTLKSPPEGDDDAR
                     LRSVDEWLPEAGGEFRHLVNAPDALATIIYTSGTTGKSKGVMLSHRNLLSNAWQALQT
                     FDVYPDDIFLSFLPLSHCLERMGGYIVPIMAGATVAYARSAQQLQEDLARIRPTVLVT
                     VPRIFERLQTSLRNKLDKGPAYARHLFALAVEVGYSRFEHAQGRAPWQRRHLLWPLLK
                     HAVAEKLAERLGGRLRLAVSGGAALSAENARTFIGLGITIIQGYGLTEASPVISVNRI
                     EDNHPTTVGNALPGVEVRLGSQDVLEVRGPNVMLGYWNNETATRTMFTTDGWLNTGDV
                     ARIDEYGHITITDRIKEIIVLNNGEKVPPTDIEAAILSDPLFEQVMLVGEGKPFLSLL
                     AVVNRERWEEAAHERDLPDIWPGGLQLQQARAFALSRVAHQMKAFPGYARIRRIVLLA
                     ELWSLDNGLLTPTLKIKRSKVLERFVHEYENLYEGYLQ"
     gene            3619..>4073
                     /locus_tag="PKW44_07160"
     CDS             3619..>4073
                     /locus_tag="PKW44_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020182972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HQR51399.1"
                     /translation="MRKTTESLAVSVALLGVCGIANAAGFGLIEQSGSGMGNAYAGAA
                     AVAEDASTIFFNPAGMTYLPDSQLVVAIHAIKPSADFSNQGSRSGVGTPMTGGDGGDA
                     GDWAFVPNLYFAKAINDSFRVGLGINAPFGLKTEYSDTLGEALDVMDQSA"
BASE COUNT          823 a         1316 c         1198 g          736 t
ORIGIN      
        1 ttctccccgg cgccgctgaa acccatcgcc gacagcgaga ttttcatgga cgaggtgctg
       61 ccggcgggcg accattccgt ttatttcatg ccggagggaa agctctccag cctgctgagc
      121 gagcaggagc acgaacaggt catgcagctg gcggacttcc atgtcatgga cagagcggcg
      181 gtcaaccgga tgaagccatg gttgctggcc gtgatgttcg accaaccgag gcccgcttcc
      241 attttctcgc aggacatcat gttacgcggg atcgcgcaga accatgccaa gccactgctg
      301 gcgctggaag agaacgaccg ccacttcctg gcgctggaca gcctcagcat ggaggagcag
      361 ctcaccatgc tgcgcaccgt cctcaagcgc agccaggagg acaaggaacg tgatttcgag
      421 tcgttgttga gcgcttattt gtccggcaac ctggcgcaga ttgccggagt ggatgaagaa
      481 atgaccggcg gcctgctgcc cgcggacatc tggcagaaaa tacgggtcaa actgctggac
      541 gaacgcaata tggaaatggc ggatcgcatc atccagcaag ccggcacaag cacggtcttc
      601 gttgccgtgg gcgcatcgca tctcgctggt agcggcggcc tattgcaacg cctgcgtagc
      661 gccgggttca ggctcagccc ggtcaagtga gcggcgatca tgcgcgcgca ttctttttct
      721 tttgtgcgga ttctcttcct cgccctgttg ttgcataccg ccgtcactcg ccacagccat
      781 gctgacgaca gcttttacga cctgcccccg cccgggcagc gtgttgatat cggcggctac
      841 aaactccaca tccattgcca aggcacaggc agcccgacag tgatcctgga tgcaggcctc
      901 ggcgactggt ccagccactg ggccagtgtt caggcactgc tcaagggcga cacccaggtt
      961 tgcagctacg accgtgccgg ctacggctgg agcgaccccg gcccacgccc ccgcgccagc
     1021 gcgcagctgg cgacggagct ccatgccctg ctgaaaaagg ccgaaatacc gccgccttac
     1081 gtcctggtcg gccattcctt cggcggcttc aacatgcgcc tgttcagcac cacgttcgac
     1141 gacgaagtcg ttgggctggt gctggtggat gcctcgcatg ccggcagcct gccttaccgc
     1201 cgcgacgaaa acggctccgg ttccaaccct gccgcctcca actaccgggt gctgatggtg
     1261 gccgcccagg ccgacatcct caagctgccg ccagaagcca ttccggccgt gcacaacgat
     1321 ttcctgcata ccaagtcgat cgccactgcc cggtcggaat accgcgccct ggcggatagt
     1381 gtcagccagg tgcagcaggc gccggggctg ggcgatattc cgctgatcgt gatttcccga
     1441 ggcatccacg aatggggaga aagcgcggaa ggcgccgcgc gtgaagcatc ctggcaggcg
     1501 cagcaggatg agctggtgcg gctctcccgc cgcggaacgc ataaaatcgc gtccaggagt
     1561 ggtcatcaca tccatctgga cgagcccgga ctggtcgcgg acgcaatacg cgaactggtg
     1621 gtcaaggcac gcgaagaaca ggttccaacc ggcggccagc cctaaggtgc agcccgcatg
     1681 gtacggcttt gcaagctgcc acgacggatt gacagctcgc agcccctcat gcacgatgcc
     1741 gtgtatccgc catcataaaa ttacaactaa aacagtaaag caatgaagtc gcggcaaact
     1801 accctgatcc cgccagaaac ggccgtcacc ctggacggcc tgtttcatga acgcgcgcgc
     1861 cgcttcccga acagcactgc ttatcagtac ttcgatcatc acctgtgcga ctggcgtgaa
     1921 ctgacctggg ggcaagtgct gcaggacatc gcccgctggc aggccgcact gatacacgaa
     1981 ggcttccagc ccggcgaccg cgtcgccatc atgctgaaaa actgtccgca atgggtgata
     2041 ttcgatctgg cggcgctggg actgggactg gtcacggtgc cgctgtatgt ttccgaccgc
     2101 ccggaaaatg tcgcccatgt gctgcaggat tccggcgcca ggctgttgtt gatcgacagc
     2161 gcggaacatt ggcacccgct gcatgaggcc tgttcgcaga tatccacgct tacccgcgtc
     2221 gtcaccctga agagtccgcc ggaaggcgac gatgatgcgc gactgcgcag cgtcgacgag
     2281 tggctgccgg aggctggcgg cgaattccgc cacctggtca atgctccgga cgcgctggcc
     2341 accatcatct acacttccgg caccaccggc aagtccaaag gcgtgatgct gtcgcaccgc
     2401 aacctgctga gcaacgcctg gcaagctttg cagactttcg acgtctaccc ggacgatatc
     2461 ttcctgtcct tcctgccgct gtcgcactgc ctggaacgca tgggcggcta tatcgtcccg
     2521 atcatggccg gcgccaccgt cgcctacgcc cgctcggcac agcaactgca ggaagatctg
     2581 gcgcgcatcc gtcccaccgt cctggtcact gtgccgcgca ttttcgaacg cctccagacc
     2641 agcttacgca acaagctgga caaggggcct gcttatgcac gccacctgtt cgcgctggcg
     2701 gtagaagttg gctacagccg cttcgaacac gcacaagggc gtgcgccgtg gcagcgcagg
     2761 cacctgctgt ggccactgct caagcatgcg gtcgccgaaa aactggcgga acggctgggc
     2821 ggcaggctac gcctggcagt ttccggcggc gccgcgctct cggcggaaaa cgcccgcacc
     2881 ttcattggcc ttggcatcac catcatccag ggttacggcc tcaccgaggc cagccctgtc
     2941 atcagcgtca accgcatcga agacaaccat ccgaccaccg tcggcaatgc cctgcccggc
     3001 gtggaggtgc ggctgggctc ccaggatgtg ctggaagtgc gcggccccaa tgtcatgctc
     3061 ggctactgga acaacgaaac cgccacccgc accatgttta ccaccgacgg ctggctcaac
     3121 accggcgacg tcgcacgcat cgacgaatac ggccacatca ccattaccga ccgcatcaag
     3181 gaaatcatcg tactcaacaa tggcgagaag gttccaccca ccgacatcga agctgcgatc
     3241 ctgagcgacc ccttgttcga gcaggtgatg ttggtgggcg aaggcaagcc cttcctgagc
     3301 ctgctggccg tggtcaaccg cgaacgctgg gaagaagcgg cgcatgaacg cgatctgccc
     3361 gacatctggc ccggcggcct gcaactgcaa caagccaggg ctttcgcgtt gtcccgtgta
     3421 gcacatcaga tgaaagcctt tcccggctat gcacgcattc gtcgcatcgt tttgctggcc
     3481 gagctgtgga gcctggacaa cggcctgctg acacctacgc ttaaaatcaa gcgcagcaag
     3541 gtgctggaaa ggtttgtaca tgaatacgaa aatttgtacg aaggttattt acaatagacg
     3601 tttacagagg gagtcagcat gagaaaaacc acagagagcc ttgcggtatc agtcgcatta
     3661 ctgggagtct gcggcattgc caacgccgcc ggcttcggat tgatcgaaca gagcggcagc
     3721 ggcatgggca acgcctatgc cggcgcggca gcggtagccg aagatgcctc caccatcttt
     3781 ttcaatccgg ccggcatgac ctatctgccg gatagccagc tggtggttgc catccacgcc
     3841 atcaagcctt ccgcggactt cagcaaccag ggctcgcgtt ccggcgtcgg cacgccgatg
     3901 actggcggcg acggtggcga tgccggcgat tgggccttcg tccccaacct ctatttcgcc
     3961 aaggcgatca acgacagttt ccgcgtcggc ctcggcatca atgccccttt cggcctcaag
     4021 accgaataca gcgacacgct gggcgaggcg ctggacgtca tggatcaatc agc
//
LOCUS       DAOMFA010000140         4046 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_3051_length_4046_cov_4.436983, whole genome shotgun sequence.
ACCESSION   DAOMFA010000140 DAOMFA010000000
VERSION     DAOMFA010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 4046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4046
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3051_length_4046_cov_4.436983"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(176..1117)
                     /locus_tag="PKW44_07165"
     CDS             complement(176..1117)
                     /locus_tag="PKW44_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HQR51400.1"
                     /translation="MHTLICGSLAYDTIMVFQDHFKHHILPEKIHILNVAFLVPEMRR
                     EFGGTAGNIAYNLNLLGGKPRIMATVGDDFANYSGWLLQNGLDTSHVREVAGSYTAQA
                     FITTDLDDNQITAFHPGAMNHSHQNRVADAADIKLGIIAPDGRDGMFQHAREFHVAGI
                     PFLFDPGQGMPMFSGEELLHFIDLADYMALNDYEAQLMQEKTGLGIEDLATRVKALIV
                     TLGAKGSVIYADGQLISIPCVEAEAVVDPTGCGDAYRAGLLYGLAQGWDWQTSGRLAS
                     LMGSIKIASRGGQNHRPSRAEIESRFKQAFATEISLA"
     gene            complement(1153..1563)
                     /locus_tag="PKW44_07170"
     CDS             complement(1153..1563)
                     /locus_tag="PKW44_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019899580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HQR51401.1"
                     /translation="MLWIKALHIIFVTSWFAGLFYLPRLFVNHAMVEDQAASERLQLM
                     ERKLYRFMTPLGVLALVFGFWLWLGYGFAGGWLHLKLILVVLLVAYHLYCGKLVQDFA
                     YGRNRHSHVWYRWFNELPVLMLFAVVILVVVKPF"
     gene            complement(1580..2128)
                     /locus_tag="PKW44_07175"
     CDS             complement(1580..2128)
                     /locus_tag="PKW44_07175"
                     /inference="COORDINATES: protein motif:HMM:NF017015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HQR51402.1"
                     /translation="MDFKQYFLYQTDYQHWANNVMLGALDRLDDEARQSTLKIYSGTI
                     HNSIDHLVFFHRKWLARLKGEQRATTYSCATHHDWRKLKENLRYELRQMQHWLERQPE
                     DFFCSSLNYQRSRSGEQQGVWVRDALTHIYTYAATERGSISLAGSMLGAPLVDMAYFT
                     YRQEMKGHVENMQKINPGSQLR"
     gene            complement(2128..2643)
                     /locus_tag="PKW44_07180"
     CDS             complement(2128..2643)
                     /locus_tag="PKW44_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HQR51403.1"
                     /translation="MTVASPNLRKWVAPLLLLILLGAMFFSFSEKKQAPEVVFTSLDG
                     QQVSMAQLRGKVVLVNFWATTCPGCIAEMPKLVQTYQRYHGKGFELVAVAMSYDPPAN
                     VASYAKNHALPFTVALDTQGDVAVAFDNVKLTPTAFLIDRQGRVVRNVVGELDFGKLH
                     QFLDKELGRAG"
     gene            2797..>4046
                     /locus_tag="PKW44_07185"
     CDS             2797..>4046
                     /locus_tag="PKW44_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3683 domain-containing protein"
                     /protein_id="HQR51404.1"
                     /translation="MTTRLREIPYNYTSFSDREIVIRFLGEDAWNTLNQLRAERKTGR
                     SARMLFEVLGDMWVITRNPYLQDDLLANRKRRDALIGALHHRLQAIDARSQNNPLVLK
                     LLEAAHRAVAAFEEEFRHTANLRKKVLARLTHITRKDNIQFDGLARVSHVTDATDWRV
                     EYPFVIVNPDTEEEVAPIVKACIELGLTLIPRGGGTGYTGGAVPLTKLSAVINTEKLD
                     RLGAIEQTGLPGVPHAVPTIRCGAGVVTRRVMEAASDAGLEFAVDPTSADACCIGGNV
                     AMNAGGKKAVLWGTALDNLASWRMVTPDATWLEVDRLDHNLGKIHDVEVARFRITRYK
                     EDGKTQTGQEILEIPGSHFRKLGLGKDVTDKFLAGLPGVQKEGCDGIITSARFILHRL
                     PPVTRTVCLEFYGQVREAVPAIVEV"
BASE COUNT          859 a         1222 c         1156 g          809 t
ORIGIN      
        1 tgcccgcgat gttcacattg cgcacactct ccaccacacc cattttcagg gtttgtgcct
       61 gctgtgcctg atcacgggtg taaacatcgc ctgccttctg gctggcgcag gctgcgagca
      121 ggctgcacag gccgagagct gcaaatagac gaaaattttt catacggata gctccttaag
      181 ccagtgatat ttctgttgca aacgcctgct tgaagcgtga ttcgatttcc gcccggctcg
      241 ggcggtggtt ttgcccgccg cggctggcga tcttgatgga ccccatcaac gatgccagac
      301 ggccgctggt ctgccagtcc cagccctgcg ccaggccgta cagcaatccg gcgcgataag
      361 catcgccgca accggtagga tcgacaaccg cttcagcctc aacgcaaggg atggagatca
      421 gttgaccgtc ggcatagatg acggagcctt ttgcccccag ggtcacgatc agcgccttca
      481 cccgcgtggc caagtcctca atgccaagac cggtcttttc ctgcatcagt tgcgcctcgt
      541 agtcattgag cgccatgtag tcagccaaat cgatgaaatg cagcagttct tcgccgctga
      601 acatcggcat gccctggccg ggatcgaaca ggaacgggat gccggccaca tggaattcac
      661 gcgcgtgctg gaacatgccg tcgcgcccgt ccggcgcgat gatgccgagc ttgatgtccg
      721 cggcatcggc cacccggttc tggtgcgaat ggttcatcgc gcccggatgg aaggcggtga
      781 tctggttgtc gtcgaggtcg gtggtgatga atgcctgcgc ggtgtagctg ccggcaacct
      841 cgcgcacgtg tgaagtatcc aggccgtttt gcagcagcca gccggaataa ttggcgaaat
      901 cgtcgccgac cgtcgccatg atcctgggct tgccacccag caggttgagg ttgtaggcga
      961 tgttgccggc ggtgccgccg aactcgcgcc gcatttccgg caccaggaag gcaacgttca
     1021 ggatatggat cttctccggc aggatgtggt gcttgaagtg atcctggaac accatgatgg
     1081 tgtcgtaagc gagcgagcca caaatgaggg tgtgcatgat gaaagttata gggttggttg
     1141 caaaaagtta agtcagaacg gttttaccac gaccaggatg accacggcga aaagcatcag
     1201 caccggcagt tcattgaacc aacggtacca gacatggctg tgccggttac ggccataagc
     1261 aaaatcctgc accagtttgc cgcagtaaag gtgataggcc accagcagta cgaccagaat
     1321 cagcttgaga tgcagccagc cgccagcgaa cccatagccc agccacagcc agaagccaaa
     1381 caccagcgcc agcacgccca gcggcgtcat gaagcggtaa agcttgcgct ccatcaactg
     1441 gagacgttca gaggctgcct gatcctccac catggcatgg ttgacgaaca ggcgaggcag
     1501 atagaacagg ccggcgaacc agctggtcac gaaaatgatg tgaagtgcct tgatccagag
     1561 catcgctagc caggatattt catcttaact ggctgccagg attgatcttt tgcatgtttt
     1621 ccacgtgccc cttcatttcc tggcgatagg tgaagtacgc catatcgacc aagggcgccc
     1681 ccagcatgct gccggccagt gaaatgctgc cgcgctcggt agcggcgtag gtatagatat
     1741 gggtcagcgc gtcacgcacc cagacgccct gctgttcgcc gctacgagaa cgctggtagt
     1801 tgaggctgct gcaaaaaaaa tcttccggct ggcgttccag ccagtgctgc atctggcgca
     1861 actcgtagcg caggttttcc ttcaatttcc gccagtcatg gtgtgtcgcg caggaatagg
     1921 tggtcgcgcg ctgttcgcct ttgagtctgg ccagccactt gcggtgaaaa aacaccagat
     1981 ggtcaatact gttatggatg gttccggaat agattttcag tgtactctgc cgcgcctcgt
     2041 catccagcct gtccagcgcc ccgagcatga cattgttggc ccaatgctgg taatcggtct
     2101 gatacaaaaa gtactgtttg aaatccatta cccagccctc cctagttcct tatccagaaa
     2161 ttggtgcagc ttgccgaaat caagctcgcc caccacgttg cgcaccaccc tgccctgccg
     2221 gtcgatcagg aacgccgtcg gggtcagctt gacattatcg aatgccacgg ccacatcacc
     2281 ctgagtgtcg agcgccaccg tgaacggcag cgcgtggttc ttggcatagc tggcgacgtt
     2341 ggcgggcggg tcgtaggaca tcgccaccgc caccagttca aaacccttgc catggtagcg
     2401 ttggtatgtc tgcaccagtt tgggcatttc ggcgatacag cccggacagg tcgtcgccca
     2461 gaagttgacc agcactacct tgccgcgcag ctgcgccatg ctcacctgct gcccatccag
     2521 gctggtgaac accacctctg gtgcttgttt tttctcggaa aaactaaaaa acatcgcccc
     2581 cagcagaatc agcagcagca gaggagcaac ccacttgcgt aaattaggag aggccacagt
     2641 cattaaggct tgattggatt gaggtatttt gttagctttc cggcaatttg tcacgccaaa
     2701 gccgtaatta aggtgaaaag gcatgatatt tcaaagggtt attcatgtat cttgatatgc
     2761 tataatactc cttttggcaa tttcctccat tcggccatga ccacccgcct gcgcgaaatc
     2821 ccatacaact atacctcttt ctccgaccgc gagatcgtca ttcgctttct tggcgaggac
     2881 gcctggaaca cactcaacca actgcgcgcc gaacgcaaga ccggccgctc ggcgcgcatg
     2941 ctgttcgagg tgctgggcga catgtgggtc attacccgta atccgtatct gcaagacgac
     3001 ctgctggcca accgcaaacg ccgcgacgcc ctgatcggcg cactgcacca ccgtctgcaa
     3061 gccatcgatg cgcgcagcca gaacaacccg ctggtgctca agctgctgga ggccgcgcac
     3121 cgggccgtgg ccgcgttcga ggaagaattc cgccacaccg ccaatctgcg caaaaaagtg
     3181 ctggcgcggc tgactcatat cacgcgcaag gataacatcc agttcgacgg gctggcgcgc
     3241 gtttcgcatg tgaccgatgc caccgactgg cgcgtcgagt accctttcgt catcgtcaat
     3301 cccgataccg aggaagaagt cgcccccatc gtcaaggcct gcatcgaatt ggggctgacg
     3361 ctgatcccgc gcggcggcgg caccggctat accggtggcg cagtgccgct gacaaaacta
     3421 tcggcggtca tcaatactga aaagctggat cgcctcggcg caatcgaaca aaccggactg
     3481 cccggcgtcc cccatgccgt gcctaccatc cgctgcggtg caggcgtagt cacgcggcgc
     3541 gtgatggaag ccgcctccga cgccggtctg gaattcgcgg tggatccaac ctcggccgac
     3601 gcctgctgca tcggtggcaa tgtggcgatg aacgccggcg gcaagaaggc cgtgctgtgg
     3661 ggcactgcac tcgacaacct ggcgtcctgg cgcatggtga caccggacgc cacttggctg
     3721 gaagtggatc gcctcgacca caacctgggc aagattcatg acgtggaggt ggcgcgcttc
     3781 cgcatcaccc gctacaagga agacggcaaa acccagaccg ggcaggaaat cctggaaatc
     3841 cccggttcgc acttccgcaa gctgggcctg ggtaaagacg tcaccgacaa gttcctcgcc
     3901 ggcctgcccg gcgtgcagaa ggaaggctgc gacggcatca tcacctcggc gcgcttcatc
     3961 ctgcaccgcc tgccgccggt gacgcgcacg gtctgcctgg agttctacgg tcaggtgcgc
     4021 gaggcggtgc cggccatcgt cgaggt
//
LOCUS       DAOMFA010000141         4038 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_3061_length_4038_cov_5.526237, whole genome shotgun sequence.
ACCESSION   DAOMFA010000141 DAOMFA010000000
VERSION     DAOMFA010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 4038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4038
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3061_length_4038_cov_5.526237"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            70..1371
                     /gene="phoR"
                     /locus_tag="PKW44_07190"
     CDS             70..1371
                     /gene="phoR"
                     /locus_tag="PKW44_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HQR51405.1"
                     /translation="MQDFRWRLLSLVIPSALLCLILWALLGESVAFCVFALILLGYLA
                     RHLYWLDKLHAWLKTPEPSTIPVGAGVWEDVFAALYQEQRSQARSQTQLTSALERFQR
                     AASALPDGVVVVNDANQIEWCNPPAELHFGLNLAQDAGKPISYMARQTEFIEYLNSQN
                     YAEPLRLKSLRHPDVTLEIQLVPFGDNQKLILCRDISLMEKLDTMRRDFIANVSHELR
                     TPLTVVGGFLETLEDMEGSVPDSTRYYFGLMQEQTNRMRRLVEDLLTLSQLESSQNVP
                     QESEVDVPALLNIVLAEAKSLSAGRHQLALQADEDLRLKGAMEELHSAFGNLVSNAIR
                     YTPEGGEIRLGWQARGAEAVFSVSDSGIGIEPQHLSRLTERFYRVDRSRSRATGGTGL
                     GLSIVKHILTRHQARLEIDSEAGKGSTFSAVFPAVRVLRKD"
     gene            complement(1368..>4038)
                     /locus_tag="PKW44_07195"
     CDS             complement(1368..>4038)
                     /locus_tag="PKW44_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HQR51406.1"
                     /translation="ILHRMPPAIRTVCLEFFGSVALAVPAIVEIKDYLDAHPHAILAG
                     LEHLDERYLKAVGYATKAQRAQRPKMVLVADIASDDENAVGEAASHIVRLANARGGEG
                     FIAVSAEARKKFWLDRARTAAIAAHTNAFKINEDVVIPLPRLGDYSDGIERINIELSI
                     NNKLKLLDFLGDFLRGDLPLVEDEDTQADAERVETRRQQALTLISDTRAHWQKVLRGL
                     DSPLQEFRLVAQELMAKAGLGEFAPLFRVVQEHHLRVSWKREVRKPLHQIFTGREYQK
                     ILERLDEIHQSVLKSRVFVALHMHAGDGNVHTNIPVNSDNYEMLREANAAVARIMQLA
                     TDLGGVISGEHGIGITKLEFLDEQTISTFADYKRKVDPEGHFNQGKLLPGADLHNAYT
                     PSFGLLELESLIMEQSEIGEIADSIKDCVRCGKCKPVCTTHVPRANLLYSPRNKILAT
                     SLLIEAFLYEEQTRRGVSLMHFDEFNDVADHCTVCHRCKNPCPVDIDFGDVSVAMRNF
                     LRKQGKKRFNPGTALGMAFLNAKDPATVKFIHTVMIQWGYKAQRTAYQVVKKLGLIGR
                     FKRRPPATLGKPEIKAQVIHFLNRPMPKNVPSKTSRALLGLEDATMVPVIRNPQKAGE
                     DANAVFYFPGCGSERLFSQVGLATQAMLYEVGAVTVLPPGYLCCGYPQTSAGNHDKGQ
                     QITTENRVLFHRVANTLNYLDIKTVIVSCGTCMDQLLKYEFEKIFPGCRLLDIHEYLM
                     EKGVKLEGVQGVRYMYHDPCHTPMKTHAPLKVANTLMGTDVALNDRCCGESGTFAASR
                     PDIATQVKMRKQEELEKGMKELGLTPGAPEQQVKILTSCPSCLQGLSRYQDDTGIEAD
                     YIVVEMAKHLLGDNWMQDYVAKASEGGIERVLL"
BASE COUNT          712 a         1204 c         1277 g          845 t
ORIGIN      
        1 gcgtcggcta ccgtttctct tcccgctagc ccataatggg cgtcctgatt atttaagcga
       61 tcaaaatccg tgcaagattt tcgctggcgc ttgttgtcgc tggtcatccc atccgctctg
      121 ctatgcctga ttctgtgggc gctattgggc gagagcgttg ctttctgcgt ttttgcgctg
      181 atcctgctgg gctacctggc acgccacctg tactggttgg ataaactcca tgcctggctg
      241 aaaacccccg agccttcgac cattccggtc ggtgccgggg tatgggaaga cgtatttgcc
      301 gcgctctacc aggaacagcg cagccaggcg cgcagccaga cccagcttac ctcggcgcta
      361 gaacgttttc agcgcgccgc aagtgctttg ccggatggtg tggtggtggt aaacgacgcc
      421 aaccagatcg aatggtgcaa cccgcccgcc gaactgcatt tcggtctcaa tctggcgcag
      481 gatgccggca agccgatcag ctacatggcc cgtcagaccg aattcatcga atatctgaac
      541 tcacagaatt acgccgagcc gctgaggctc aaatcgctgc gtcatcccga tgtcacgctc
      601 gaaatccagc tggtgccgtt cggcgacaac cagaagctga tcctgtgccg cgacatttct
      661 ctcatggaaa agctggacac catgcggcgc gacttcattg ccaatgtttc gcatgagcta
      721 cgcacgccgt tgacggtggt gggcggcttt ctggaaacgc tggaagacat ggaaggcagc
      781 gtgccggaca gcaccaggta ttattttgga ttgatgcagg aacagaccaa ccgcatgcgc
      841 cgcctggtgg aagacctgct cacgctgtcg cagctggaga gcagccagaa cgtgccgcag
      901 gaaagcgagg tcgacgtgcc ggcgctgctg aatatcgtct tggcggaagc gaaaagcctg
      961 agtgccggcc gccaccagct tgccctgcaa gccgatgaag acctgcgttt gaaaggcgcc
     1021 atggaagaat tgcacagtgc tttcggcaat ctggtcagca atgccattcg ctacacgccg
     1081 gaaggcggtg aaatcaggct cggttggcag gcgcgcggtg cggaagcggt gttcagcgtc
     1141 agcgacagcg gtatcggcat cgagccgcaa cacctctccc gcctcaccga acgcttctac
     1201 cgcgtcgacc gtagccggtc gcgcgccacc ggcggcaccg ggctgggcct gtccatcgtc
     1261 aagcacatcc ttacccgcca tcaggccagg ctggaaatcg acagcgaagc gggcaagggc
     1321 agcaccttca gcgccgtatt tccagcggtg cgggtgctac gcaaggatta gagcaacacc
     1381 ctttcgatcc cgccttcgct ggccttggcc acgtagtcct gcatccagtt atcgcccagc
     1441 agatgcttgg ccatttccac cacgatgtag tcggcttcaa tcccggtgtc gtcctggtag
     1501 cgtgacaggc cttgcaggca ggacgggcag gaggtgagga tcttgacctg ctgttccggc
     1561 gcgccggggg tgaggccgag ctctttcatg cctttttcca gttcttcctg cttgcgcatc
     1621 ttgacctggg tggcgatgtc cgggcgcgag gcagcgaagg tgccggattc gccgcagcag
     1681 cggtcgttga gggcgacgtc ggtgcccatc agggtgttgg ccaccttgag cggggcgtgg
     1741 gtcttcatcg gcgtgtggca ggggtcgtgg tacatgtagc gcacgccctg cacgccttcc
     1801 agcttgacac ctttttccat caggtattcg tggatgtcga gcaggcggca gccggggaat
     1861 atcttctcga actcgtactt cagcaattga tccatgcagg tgccgcagga cacgatgacg
     1921 gtcttgatgt cgaggtaatt gagggtgttg gccacgcggt ggaacagcac ccggttttcc
     1981 gtcgtaatct gctggccctt gtcgtggtta ccggccgaag tctgcgggta gccgcagcac
     2041 agatagcccg gcggcagtac ggtgacggcg ccgacttcgt acagcatggc ttgcgtggcg
     2101 aggccgacct gcgagaacag gcgctccgag ccgcagccgg ggaagtagaa caccgcgttg
     2161 gcgtcttccc ccgctttttg cgggttgcgg atcaccggca ccatggtggc gtcctccagc
     2221 cccagcagcg cgcgcgaggt cttggacggc acgttcttgg gcatcggtcg gttgaggaag
     2281 tggatcacct gcgccttgat ttccggcttg cccagcgtgg cgggcgggcg gcgcttgaag
     2341 cggccgatga gccccagctt cttgaccacc tggtaagcgg tgcgctgcgc cttgtagccc
     2401 cactggatca tcaccgtgtg gatgaacttg acggtggccg ggtccttggc gttgaggaag
     2461 gccatgccca gcgcggtgcc ggggttgaag cgcttcttgc cttgtttgcg caaaaagttg
     2521 cgcatggcga cggatacgtc tccgaagtcg atgtccaccg ggcacgggtt tttgcagcga
     2581 tggcacaccg tgcagtggtc ggctacgtcg ttaaactcgt cgaagtgcat cagcgacaca
     2641 ccgcgtcggg tctgctcctc gtacaaaaac gcctcgatca gcaggctggt cgccaagatc
     2701 ttgttgcgcg gtgaatacag caaattggcg cgcggcacgt gggtggtgca taccggcttg
     2761 cacttgccac agcgcacgca gtccttgatc gaatcggcga tctcgccgat ttccgactgc
     2821 tccatgatga gcgattccag ttccagcagg ccgaagctgg gcgtgtaggc gttatgcaag
     2881 tcggcgcccg gcagcagctt gccctggttg aaatggcctt ccgggtcgac cttccgcttg
     2941 tagtcggcga aggtactgat ggtctgctcg tccaggaatt ccagcttggt gataccgatg
     3001 ccgtgttcgc cggagatcac cccgccaagg tcggtggcaa gctgcatgat gcgcgccacc
     3061 gccgcgttgg cctcgcgcag catttcatag ttatcggaat tgaccgggat gttggtgtgc
     3121 acgttgccgt cgccagcatg catgtgcagt gccacgaata cgcggctctt cagtaccgat
     3181 tggtgaatct cgtccaggcg ctccagtatt ttctgatact cgcggccggt gaaaatctgg
     3241 tgcaagggtt tacgcacctc gcgcttccag gacacgcgca aatggtgctc ctgcactacg
     3301 cggaacagtg gcgcaaactc gcctagcccg gctttcgcca tcagttcctg cgctaccagg
     3361 cggaattcct gcagcggcga atccaggccg cgcagcacct tctgccagtg agcgcgggta
     3421 tcgctgatca gggtcagtgc ctgttgacgc cgggtttcca cccgctcggc gtcggcttgc
     3481 gtgtcctcgt cttccaccag cggcaggtcg ccgcgcagga agtctcccag gaaatccagc
     3541 agcttgagct tgttattgat cgacagttcg atgttgatac gctcgatgcc gtcggagtaa
     3601 tcccccaggc gcggcagcgg gatgaccacg tcctcgttga tcttgaaggc gttggtgtgg
     3661 gcggcgatgg cggcggtacg ggcgcggtcc agccagaatt tcttgcgcgc ctcggcgctg
     3721 acggcgataa aaccttcgcc gccgcgtgcg ttggccaggc gcacgatgtg cgaggcggcc
     3781 tcacccacag cgttttcgtc gtcgctggcg atgtccgcga ctagcaccat cttgggccgc
     3841 tgcgcacgct gcgctttggt ggcgtagccg acggccttca ggtagcgctc gtccagatgt
     3901 tccagtccgg ccaggatggc gtgcgggtgg gcatcaaggt aatccttgat ctcgacgatg
     3961 gcaggcactg ccagtgctac cgagccgaag aattccaggc acaccgtgcg gatcgccggc
     4021 ggcatgcggt gcaggatg
//
LOCUS       DAOMFA010000142         3981 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_3128_length_3981_cov_4.430718, whole genome shotgun sequence.
ACCESSION   DAOMFA010000142 DAOMFA010000000
VERSION     DAOMFA010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 3981)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3981)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3981
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3128_length_3981_cov_4.430718"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1189
                     /locus_tag="PKW44_07200"
     CDS             <1..1189
                     /locus_tag="PKW44_07200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR51407.1"
                     /translation="PSLAGPHYPEGIPIVEEVALEALCHERQVNQVVFAYSDLPHVEV
                     MHLASRALSCGADFVLLGPDRTMLQSRLPVIAVSAVRTGCGKSQTARWLSCLLREKGL
                     RVAVLRHPMPYGDLQREAVQRFASRADLDAADCTLEEREEYEPHLAIGNVVYAGVDYR
                     SILQLAEQHADLILWDGGNNDFPFLRPDLHIVLVDPLRHGDELLFHPGETVLRMADII
                     VVVKTDTARTDAIEQTEANARHANPHAAIVRAASPISLDGNPDLRNRRVLVVEDGPTT
                     THGGMAFGAGYLAASHAGADIIDPRPCAAPAIAAVYAQYPHIGPVLPAMGYSGEQLAA
                     LRQTIADTDAEVVVSATPIDLAALLELNKPVVRTRYEFSEASAPGLHERVERFLTERL
                     LLP"
     gene            1246..2319
                     /gene="corA"
                     /locus_tag="PKW44_07205"
     CDS             1246..2319
                     /gene="corA"
                     /locus_tag="PKW44_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HQR51408.1"
                     /translation="MKKSRRNRSKKVGLPPGALVSMGEVKLKHAQLSLFRYDETDIHE
                     QNELDLSGFDLQAPQNGTLWLNIHGLHDTALMAEIGRAFTLHPLVLEDILNTDQRPKL
                     DSYDDYLFFVTRFFHYDQAGMTISSEQVSLILGRNFVLTFQERPTGSFDPVRERLRAD
                     KGHIRKAGADYLAYALLDIIVDRYFVVLEQISDDCEALEEELLRKPGASMLQSIHKLK
                     RETMELRRAVWPLREVVNALIRNEPGFFQPGTLIYLRDVYDHTVHFIESLESLRDLLA
                     GMLDIYLSSISNRVNMELRALTVVAMLFMPATLIAGIFGMNFKVMPWLSDTNGFWFAM
                     GLMGGIASLMGMIFWRRQWLSRR"
     gene            complement(2306..2851)
                     /gene="msrA"
                     /locus_tag="PKW44_07210"
     CDS             complement(2306..2851)
                     /gene="msrA"
                     /locus_tag="PKW44_07210"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HQR51409.1"
                     /translation="MSNSQTITLAGGCFWCLEAVFRQIKGVRRAVSGYIGGHVADPDY
                     KGVCTGTTGHAEAVQLTFDPRLISLQELLEVFFSSHDPTTRNRQGNDIGTQYRSAIFY
                     HSAEQKQIARQFIAGLDAAHFWPNSVVTEIMPAGTFYPAEGYHQDYFANNAHQSYCQL
                     VVAPKVTKVRQYFSEKVISAD"
     gene            2942..>3981
                     /locus_tag="PKW44_07215"
     CDS             2942..>3981
                     /locus_tag="PKW44_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HQR51410.1"
                     /translation="MSLNALYVDFNSYFASVEQQLRPELRGCPIGVLPVMAETTCCIA
                     ASYEAKAFGVKTGTMVRDARKLCPDIILVGARPAIYIDFHHRLVDVVESCTPVGKILS
                     IDEMVCRLTGSQQSRDHALALAAQIKQSISKKVGSEIRCSIGIAPNTFLAKLASNMQK
                     PDGCVVIEQHELPDRLYSLELRDLTGIGRRMEQRLTAKSITTIRSLYALNRQQMRAAW
                     GSVEGEYIYDKLRGLEPPESGHGRSSLGHSHVLPPEMRNREAALSVLNRLLQKACARL
                     RSYQLYASMIHIRVKFPRRPTWEADGACSPTDDTLQLIHALQGLWRQYPDHTDTPYAV
                     GISLSGLVGARG"
BASE COUNT          801 a         1261 c         1132 g          787 t
ORIGIN      
        1 gccgtccctg gctggcccac attatccgga gggcatccct atcgtcgaag aagttgcgct
       61 ggaagccctc tgccacgaac gacaagtcaa ccaggtcgta ttcgcctaca gcgaccttcc
      121 gcatgtcgaa gtgatgcacc ttgcctcgcg cgcgctgtcc tgcggcgccg atttcgtgct
      181 gctggggccg gaccgcacca tgctgcaatc caggctgccg gtgatcgccg tttccgccgt
      241 gcgtaccggc tgcggcaagt cgcagaccgc gcgctggctg tcgtgcctgc tccgcgaaaa
      301 gggcctgcgc gtggccgtat tgcgccatcc catgccctac ggcgacctgc agcgcgaggc
      361 agtgcagcgc tttgccagcc gtgctgacct ggacgctgct gactgcacac tggaagaacg
      421 cgaggaatat gagccgcacc tggcgattgg caacgtcgtc tatgccgggg tggattaccg
      481 cagcatcctg cagctggcgg aacagcacgc cgatcttatc ctgtgggatg gcggcaacaa
      541 cgactttccc ttcctgcggc ccgacctgca tatcgtgctg gtcgatcccc tgcgtcacgg
      601 cgacgagctg ctgttccatc cgggcgaaac cgtgctgcgc atggccgaca tcatcgtggt
      661 ggtcaaaacc gataccgccc gaacagatgc catcgaacaa accgaagcca atgcgcgcca
      721 tgccaatcct catgccgcaa tcgttcgcgc cgcctcgccc atcagcctgg acggcaatcc
      781 ggacctgcgc aaccggcgcg tgctggtggt ggaagacggc ccgaccacta cacacggtgg
      841 catggcgttc ggcgccggct acttggccgc ctcacatgct ggcgccgaca tcatcgatcc
      901 gcgcccctgc gccgcaccgg ctattgctgc agtctatgcg caatatccac acatcggccc
      961 ggtgctgccg gccatgggct actccggcga gcaactggcg gcgctgcgcc aaaccatagc
     1021 cgacaccgat gccgaagtgg tggtgtctgc tacgccgatc gacctcgccg cgctgctcga
     1081 actcaataaa cccgtggtgc gcacccgcta cgaattctcc gaggcaagtg ctcccgggct
     1141 gcacgaacga gtggagcgct tcctgaccga gcgacttctg ctaccataag ccgatccatc
     1201 ccacgtcgat gacgaggcag ccgttatgac cctttgccac tccccatgaa aaagagccgt
     1261 cgcaaccgtt caaaaaaagt cggcctgcca ccgggcgcgc tggtgtccat gggtgaggta
     1321 aaactcaagc atgcgcaact gtcgctgttc cgctatgacg aaactgatat ccacgagcag
     1381 aacgagctgg atttatccgg cttcgacctg caggcaccgc aaaatggcac gctgtggctc
     1441 aacatccacg gcctgcacga caccgcattg atggccgaga tcgggcgcgc cttcaccctg
     1501 cacccgctgg tgctggaaga catcctcaat accgaccagc gtcccaaact ggacagctat
     1561 gacgactacc tgtttttcgt cacccgcttc ttccactacg accaagcagg catgaccatc
     1621 agttcggaac aggtcagcct gatcctcggc cgcaatttcg tgctgacctt ccaggagcgc
     1681 cccactggct ccttcgaccc ggtgcgcgag cgcctgcgtg ccgacaaagg ccacattcgc
     1741 aaggctggcg ccgactacct ggcttatgcg ctgctggata tcatcgttga ccgctatttc
     1801 gtcgtgctcg aacagatcag cgacgactgc gaagcgctgg aggaagaact gctgcgtaaa
     1861 cccggggcct ccatgttgca gagcatccac aaactcaagc gcgaaaccat ggagttgcgg
     1921 cgcgcggtat ggccgctacg cgaagtcgtc aatgcgctga tccgcaatga acccggcttc
     1981 ttccagccgg gaacgctgat ctacctgcgc gacgtctatg accacaccgt gcatttcatc
     2041 gagtcgctgg agtccctgcg ggatttattg gccggcatgc tggatattta cctgtccagc
     2101 atcagcaacc gcgtcaacat ggaattgcgc gcgctgaccg tggtcgccat gctgttcatg
     2161 ccggccacgc tgattgcggg catcttcggc atgaacttca aggtcatgcc gtggctctcg
     2221 gacactaatg gattctggtt cgccatgggc ctgatgggcg gcattgccag cctgatgggg
     2281 atgattttct ggcgccgcca gtggctcagt cggcgctgat gaccttttca gaaaagtact
     2341 gccgcacctt ggtcactttg ggcgccacaa ccagctggca ataagactga tgggcattgt
     2401 tggcgaagta gtcttggtgg tagccctcgg ccgggtaaaa ggtgcccgcc ggcatgattt
     2461 ctgtcaccac cgagttcggc cagaaatgtg ccgcatccag cccggcgatg aactgtcgtg
     2521 cgatctgttt ttgctccgca ctatggtaaa aaatagctga gcggtactgc gtgccgatgt
     2581 cattgccctg ccgattgcgg gtagtcggat cgtggctgga gaaaaatacc tccagcaatt
     2641 cctgcaggga aatcaggcgg gggtcgaagg taagctgcac cgcctcggca tggcctgtag
     2701 tcccggtgca gacccccttg tagtcggggt cggccacatg cccgccgata tagccggaga
     2761 ctgcgcgccg cacgcctttg atctggcgga ataccgcttc caggcaccag aagcagccac
     2821 ctgccagggt gatggtttga gaattggaca ttttgcaact cctgccttgt tgagaaccat
     2881 cccttagtcg cttcgggtac actttactga cattctgacc tgaaatttta catcgcacgc
     2941 catgtcgctc aacgcacttt acgtcgactt caactcctac ttcgcttcag tggagcagca
     3001 gttgcgcccg gaactgcgcg ggtgccccat cggcgtgctg ccggtcatgg ccgaaaccac
     3061 ctgctgcatc gccgccagtt acgaagccaa ggcgttcggc gtaaaaaccg gcaccatggt
     3121 gcgagatgcc cgcaaactct gccctgacat tattctcgtt ggggcacgcc ctgcaatata
     3181 catcgacttc caccaccgac tggtggatgt ggtggaatcc tgcacaccag taggaaaaat
     3241 cctgtccatc gacgaaatgg tatgccgtct gaccggtagc cagcaatcgc gcgaccatgc
     3301 actcgctctg gcggcgcaga tcaagcaatc tatctccaaa aaggtgggca gtgaaatccg
     3361 ttgttccatc ggcattgcac ccaacacctt tcttgccaag ctcgcttcca acatgcagaa
     3421 gccggacggc tgcgtggtca tcgaacagca cgaactgccc gacaggcttt attcactcga
     3481 attgcgcgac cttaccggca tcggcaggcg catggagcag agattgacgg ccaagagcat
     3541 tactacgatc aggtcgctgt acgcgctgaa ccgccagcaa atgagggccg cctggggcag
     3601 cgttgagggt gagtacatat acgacaagct gcgcgggctg gagccgcctg aatccgggca
     3661 tgggcgtagc agcttgggac attcacacgt attgcctcca gaaatgcgca accgggaagc
     3721 agctctttca gtattgaacc gcctgctgca aaaagcctgc gcgcggctgc gcagctacca
     3781 gctttatgcc tcgatgatcc atatccgggt caagtttccg aggcgcccga cgtgggaggc
     3841 agacggcgcc tgctcaccca ccgacgatac gctacagctg attcatgccc tgcagggtct
     3901 atggcggcaa taccctgacc acacggacac gccatacgca gtgggaattt cattgtccgg
     3961 cctggtcggg gcgcgggggt t
//
LOCUS       DAOMFA010000143         3954 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_3151_length_3954_cov_5.239036, whole genome shotgun sequence.
ACCESSION   DAOMFA010000143 DAOMFA010000000
VERSION     DAOMFA010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 3954)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3954)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3954
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3151_length_3954_cov_5.239036"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..609
                     /locus_tag="PKW44_07220"
     CDS             <1..609
                     /locus_tag="PKW44_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase large subunit"
                     /protein_id="HQR51411.1"
                     /translation="LPVVSKENLPRVWAALDMPSGYAYGKSIRTVKTCVGSEFCRFGT
                     QDSTRMGIELEKAFDHMWAPHKVKLAVSGCPRNCAESGIKDVGIIGVDSGWEIYVGGN
                     GGIKTEAAQFLCKVKTHEEVMEYSGAFIQLYREEARYLDRTVHWIARIGLDYVKQRVL
                     EDHENRKALYERLLYALQGATDPWAEQVRDANKRRQFEIIAV"
     gene            639..1844
                     /locus_tag="PKW44_07225"
     CDS             639..1844
                     /locus_tag="PKW44_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HQR51412.1"
                     /translation="MKSSFWNAGHKPTLFSAFLYFDLSFMVWVLLGPLGVQIAATLNL
                     TAAQKGMMVATPVLAGAVLRMLLGVLVDHIGPKKTGLIGQVVVMAALLAAWLHGIENY
                     HQALLLGVFLGMAGASFAVALPLASRWYPPEHQGTALGIAGAGNSGTALGALFAPGLA
                     VAYGWNNVFGLALIPLALVFVVYAIYAKDSPDTPPPKTLGQYLAVLKDRDAWWFMLFY
                     SVTFGGFVGMASSLTIYFHDQYGLSPVIAGFFTAACVFAGSLMRPLGGNVADRVGGIR
                     TLTLVYGFAALFLFLLSFNPADKLVGLAVVVAAMLSLGMGNGAVFQLVPQRFRKEIGV
                     MTGLVGMAGGIGGFYLASSLGYSKQLTGSYQIGLIAFACLAILAMIGLTSVKTRWRTT
                     WGAATSAKV"
     gene            1855..3549
                     /locus_tag="PKW44_07230"
     CDS             1855..3549
                     /locus_tag="PKW44_07230"
                     /EC_number="2.7.11.-"
                     /EC_number="3.1.3.-"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-serine/threonine
                     kinase/phosphatase"
                     /protein_id="HQR51413.1"
                     /translation="MLRLTIGERSDQGPRERNEDFYGVVTPENEQLVAKGALLAVADG
                     VSGHSGGREAAEMSVRSLLSDYYATPDTWEVTVALDKVLGAVNRWVLAQAAKQREMAG
                     MATTLSALVLRGRRYTLAHVGDSRVYRLRDGALQLLTTDHVWDREDMRHVLKRAIGLD
                     RQLTVDYAEGELQVGDVFALVTDGVWERLGTARIQKILALYQNPQLAADDLVKSALAE
                     GSEDNATALVTRVEQVGEENFADLLAGAKTLGLPPRLKPGERLDDFEVLDLLHESRAS
                     LIYKARHVETQQLCVIKTLQPLLAHDMESCTGLLNEEWLAKRVVAHFFPQVLPIPMER
                     RSCLYYVMSFHAGATLQKQLDSGVHFTAADVAGIGIRLVKGLAVLHRLNIVHRDIKPA
                     NVHMGEDGRLRILDLGVSLNAGMSTTSKGGSNPGTPSYMAPELFDGESATVQTDLYAV
                     GVTLYHLLTRKYPYGEIEPFQHPRFGDPVAPTRYRPEIPQWLENIVLKAVTADPARRF
                     ETAEEMLLALELGEARPILAPGRTPIAERRVSGGWQLVAMLSVLVNILLIYLLIIS"
     gene            3566..3877
                     /gene="nirD"
                     /locus_tag="PKW44_07235"
     CDS             3566..3877
                     /gene="nirD"
                     /locus_tag="PKW44_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008942 - nitrite reductase [NAD(P)H]
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HQR51414.1"
                     /translation="MSEWGKIVALEEIPRLGSRIIKRSGGSDIAVFRTADDKVFALHD
                     KCPHKGGPLSQGIVHGEKVTCPLHGWNIGLGDGQAIAPDVGSTPCVEAKLEDGMVYLK
                     L"
BASE COUNT          709 a         1160 c         1319 g          766 t
ORIGIN      
        1 ctgccggtcg tcagcaagga gaacctgccc agggtgtggg ccgccctcga catgccttcc
       61 ggctacgcct acggcaagtc gatccgcacc gtgaagacct gcgtcggctc agagttctgc
      121 cgcttcggca cgcaggattc gacgcgcatg ggcatcgaac tggaaaaagc cttcgaccac
      181 atgtgggcgc cacacaaggt caagctggcg gtgtccggct gcccgcgcaa ctgcgccgaa
      241 tccggcatca aggacgtggg catcatcggt gtcgattccg gctgggaaat ctatgtcggc
      301 ggcaacggcg gcatcaagac cgaggcggcg cagttcctgt gcaaggtgaa aacgcacgag
      361 gaagtgatgg agtattccgg tgctttcatc cagttgtacc gggaggaagc gcgctatctc
      421 gaccgtaccg tgcactggat cgctcgcatc gggttggact acgtcaagca gcgcgtgctg
      481 gaagaccacg aaaaccgcaa ggcactgtac gaacgtctgc tgtatgcgct gcagggtgcg
      541 accgatccgt gggccgagca ggtgcgcgac gcgaacaagc gccgccagtt cgagatcatc
      601 gccgtctgag ttttaatttc actttcccgg agtatcgtat gaaaagcagt ttctggaatg
      661 ccggccacaa gccgaccctg ttttccgcat ttttatattt cgacttgagt ttcatggtgt
      721 gggtgctgct cggcccgttg ggcgtgcaga tcgctgccac gttgaacctc accgctgcac
      781 agaagggcat gatggtcgct acgccggtac tcgccggcgc cgtgttgcgc atgctgttgg
      841 gcgtgctggt ggatcacatc ggccccaaga agaccggcct gattggccag gtggtggtga
      901 tggcggcctt gctagcggcc tggctgcatg gcatcgaaaa ttaccaccag gccctgctgc
      961 tgggcgtatt cctcggcatg gcgggcgctt cgttcgccgt cgccctgccg ctggcctcgc
     1021 gctggtatcc gcccgagcat cagggcacgg cgctgggcat cgccggggca ggcaattccg
     1081 gcacggcgct gggtgcactg ttcgcccccg ggctggcggt agcctacggc tggaacaacg
     1141 tattcggcct ggcactgatt ccgctggcgc tggtgttcgt ggtttatgcc atttacgcca
     1201 aggacagccc ggataccccg ccgcccaaga ccctgggcca gtacttggcg gtgctgaaag
     1261 acagggatgc ctggtggttc atgctgttct atagtgtgac ttttggtgga ttcgtcggca
     1321 tggcctcatc gctcaccatt tatttccacg accagtacgg cctgagcccg gtgatcgccg
     1381 gattcttcac cgccgcctgc gtcttcgccg gttcgctgat gcgcccgctg ggcggcaacg
     1441 tcgccgaccg ggtcggcggg atccgcacgc tgaccctggt gtacggcttc gccgccctgt
     1501 tcctgttcct gctcagcttc aacccggcgg acaagctggt ggggctggcc gtcgtggtcg
     1561 ccgccatgct ctcactcggc atgggcaacg gcgccgtgtt ccagctggtg ccgcagcgct
     1621 tccgcaagga aatcggcgtg atgaccggcc tggtcggcat ggcgggcggc atcggcgggt
     1681 tctacctggc ttccagcctg ggctattcca agcagctcac cggcagctac cagatcggcc
     1741 tgatcgcttt tgcctgcctc gcgatcctgg ccatgatcgg cctgaccagc gtcaagacgc
     1801 gctggcgtac cacttggggc gcggccacca gcgccaaggt gtaggctttt ttcgatgctc
     1861 cggctgacca taggcgaacg cagcgaccag gggcctcgcg aacgcaacga ggacttctac
     1921 ggtgtcgtca cgccggaaaa cgagcagctc gttgccaaag gcgcgttgct ggcggtggcg
     1981 gatggcgtaa gcgggcattc cggcgggcgc gaagcggccg agatgtcggt gcgcagcctg
     2041 ctgtcggact actatgccac gcccgacacc tgggaagtca ccgtggcgct ggacaaggtg
     2101 ctgggtgcgg tcaaccgctg ggtgctggcg caggccgcca agcaacgcga gatggcgggc
     2161 atggcgacca ccttgtcggc cctggtgctg cgcgggcggc gctacaccct ggcgcacgtc
     2221 ggcgacagcc gggtgtaccg gttgcgtgac ggtgcgctgc aattgctcac caccgaccac
     2281 gtgtgggacc gggaggacat gcgccacgtg ctgaagcgtg ccatcggcct ggatcgccag
     2341 ttgaccgtgg actatgccga gggggaattg caagtgggcg acgtgtttgc cctggtgacg
     2401 gatggcgtgt gggaaaggtt gggtacggcg cgtatccaga aaatcctggc gctgtaccag
     2461 aatccgcaac tggcagccga cgacttggtg aagagcgcgc ttgcggaagg gagtgaagat
     2521 aacgcgacgg cgctggtgac gcgcgtggaa caggtaggcg aggagaattt cgccgatctg
     2581 ctggctggcg cgaaaacgct ggggctgccg cccaggctca agcccggcga gcggctggat
     2641 gatttcgagg tgctggatct gctgcacgag tcgcgcgcca gcctgattta caaggcccga
     2701 catgtggaaa cgcagcagct gtgcgtaatc aagaccctgc agcccttgct ggcgcacgac
     2761 atggaaagct gcaccggcct gctcaacgag gaatggctgg cgaaacgggt agtggcgcat
     2821 ttcttcccgc aggtgctgcc catccccatg gaacggcgca gctgcctcta ctacgtgatg
     2881 agtttccacg cgggggcgac cttgcagaaa cagctcgaca gcggcgtgca tttcacggcg
     2941 gcggacgtcg ccggcatcgg catccggctg gtcaaggggc tggccgtgct gcaccggctc
     3001 aatatcgtgc atcgcgacat caagccggcc aatgtgcaca tgggtgagga tggccgtctg
     3061 cgcatcctcg acctcggcgt gtcgctcaat gccggcatga gcaccaccag caagggcggc
     3121 agcaaccccg gtacgcccag ctacatggcg ccggaactgt tcgacgggga aagcgccacg
     3181 gtgcagaccg acctctatgc ggtcggggtg acgctctacc acctgctgac acgcaagtac
     3241 ccttacggcg agatcgaacc gttccagcac ccgcgcttcg gcgatcccgt ggcgcccacg
     3301 cgctaccgcc cggaaatccc gcaatggctg gaaaatatcg tgctcaaggc ggtgacggcc
     3361 gatcctgcca ggcggtttga aacggccgag gaaatgctgc tggcactaga actgggcgag
     3421 gccagaccta tcctggcgcc agggcgtacg ccgatagccg agcggcgcgt ctcggggggc
     3481 tggcaactgg tcgccatgct gtcggtttta gtgaatatcc tgttgattta tttgctgatt
     3541 atttcttgaa aacgttggag acatcatgag cgagtgggga aaaatcgttg ccctggagga
     3601 gattcccaga ctgggttcgc gcatcatcaa gcgcagtggg gggagtgaca ttgcggtgtt
     3661 ccgcacggca gacgacaagg tgtttgcgtt acatgacaaa tgtccgcaca agggcgggcc
     3721 gctgtcgcag ggcatcgtgc atggcgagaa ggtcacctgc ccgttgcacg gctggaatat
     3781 cggcttgggc gacggtcagg ccatcgcccc ggatgttggc agcacaccct gtgtcgaggc
     3841 gaagctggaa gacggcatgg tctatttgaa gttgtagttg ttttttatgg aatctttaga
     3901 ggagaatcaa atggcagtga ttgatcgtgt attagtgggt gaggcgcttt atat
//
LOCUS       DAOMFA010000144         3881 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_3254_length_3881_cov_3.923157, whole genome shotgun sequence.
ACCESSION   DAOMFA010000144 DAOMFA010000000
VERSION     DAOMFA010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 3881)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3881)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3881
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3254_length_3881_cov_3.923157"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..293
                     /locus_tag="PKW44_07240"
     CDS             <1..293
                     /locus_tag="PKW44_07240"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985329.1"
                     /note="catalyzes the formation of oxaloacetate from
                     pyruvate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate carboxylase subunit B"
                     /protein_id="HQR51415.1"
                     /translation="RTPAPPRSGAQRYAVPLHGSRPRPSHEGCVTTAMPGTIVDVKVK
                     AGDTVAAGDGVLVIEAMKMENEIQSPKSGIVIAVHVKKGDSVTPDETLIEIQ"
     gene            330..908
                     /locus_tag="PKW44_07245"
     CDS             330..908
                     /locus_tag="PKW44_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family nucleotide pyrophosphatase"
                     /protein_id="HQR51416.1"
                     /translation="MQNLILASSSIYRRELLQHLQLPFTVVSPDVDETPLPQERPEQT
                     ALRLAQEKARKIALTHPDVLVIGCDQVATLDGQQLGKPLIHDTAVKQLQLMRGRTVTF
                     HSALCLYNAVSGSMQAEVVPYDVRFRQLTDEQIEYYLLKEQPYHCAGSAKSEGLGIAL
                     IEWMRGDDPNALIGLPLIRLVGMLRNEGVTVI"
     gene            962..>3881
                     /locus_tag="PKW44_07250"
     CDS             962..>3881
                     /locus_tag="PKW44_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HQR51417.1"
                     /translation="MPLSWNEIKSRATAFSRNWADAGNEDSQAKPFLIDFFEVFGITD
                     KRVASFEHAVAKHGGGRGKVDLFWPGILLVEMKSRGKNLDRAFDQAMDYFPGIAERDL
                     PRYVLVCDFARFRLYDLTTGDTSEFALKDLYQNARQFGFIAGYQTQVIKPQDPINIKA
                     AERMGRLHDQLKAVGYVGRPLEIYLVRLLFCLFAEDTGIFDKRQFQDYVENRTGEDGS
                     DLAHHLGTLFYVLNTPHDKRLKNLDEDLAAFPYVNGRLFEEMLPPASFDGKMRDALLD
                     LCALDWSLISPAIFGSLFQSIMDDKARRNLGAHYTSEENILKLIKPLFLDALWAEFDK
                     VKGNKNRLFEFHKKLRTLTFFDPACGCGNFLVITYRELRLLELEVLRASRASGQQVLD
                     VQQLINVDVDQFHGIEIEEFPAQIAQVALWLTDHQMNMRVSEEFGSYYARIPLKATPH
                     IVHGNALWLDWAEVLVPERCAYVLGNPPFVGAKFMDDAQREDARLVFAGIDNAGLLDF
                     VACWYVKAARYINTPRPTSWGEGPGVRGEPLRRDPAIEEKLKTYARELRASATDAEQK
                     LWHLLRDRRFFDFKFRRQHPVAGYILDFYCAEAKFAIELDGGQHAQAEEYDAQRTHTL
                     AQQGIRLLRFWNNEFLQNPEGVLETIATALQVRPSPSIPLPATQGEGSRIRCAFVSTN
                     SITQGEQVGALWGWLLSQGVHIHFAHRTFSWSNEARGKAAVHCVIVGFGSGERADKLI
                     YDYDDIKGEPHAIPARNINPYLVDAPDMVLPRRSKPICNVPEIGIGNKPIDGGHYLFT
                     PEEKAQFLLKEPTAAPYFRRWIGADEFINGYERWCLWLGDCPPHQLRAMPEAMKRVDM
                     VRRFRLGEGINTKGKKAEKAPPESTRKLAQTPTRFHVENIPFTPYLVLPEVSSERRAF
                     IPIGYEQPETLSSNLVRILPDATLFHFGVLTSTMHNAWVRSVCGRLKSDFRYSISIVY
                     N"
BASE COUNT          815 a         1269 c         1108 g          689 t
ORIGIN      
        1 cgcgaacccc ggcgccaccg cggtcgggcg cgcagaggta cgcggtgcct ttgcacggca
       61 gccgtccgcg gccgagccac gaaggctgcg tcaccaccgc catgcccggc accatcgtcg
      121 acgtcaaggt caaggccggc gatacggtcg ccgccggcga cggcgtgctg gtgatcgaag
      181 ccatgaagat ggaaaacgag atccagtcgc ccaagagcgg catcgtcatc gccgtccatg
      241 tcaagaaggg cgacagcgtg acgccggacg aaaccctgat cgagatccag taagcctcat
      301 gaggccggcg caagccggcc ttttacttta tgcagaacct gatcctcgct tcttcctcca
      361 tctaccgccg cgaactgctg cagcacctgc agctgccgtt cacggttgtc tcccccgatg
      421 tggacgaaac cccgctgccg caggaacgtc cggagcagac cgcgctgcgg ctggcgcagg
      481 aaaaagcccg caagatcgcg ctgacgcacc ccgacgtcct cgtcatcggc tgcgaccagg
      541 tcgccaccct ggacggccag cagctcggca agccgctcat tcacgacacc gccgtcaaac
      601 aactgcaact gatgcgcggc cgcacggtca cctttcacag cgccctgtgc ctgtacaacg
      661 ccgtcagcgg cagcatgcag gccgaagtgg tgccctacga cgtgcgtttc cgccagctta
      721 ccgacgagca gatcgagtat tacctgctca aggaacagcc ctaccactgc gccggcagcg
      781 ccaaatccga gggcctgggc atcgccctga tcgaatggat gcgcggcgac gaccccaacg
      841 cgctgatcgg cctgccgctg atccggctgg tcggcatgtt gcggaacgaa ggcgtcacgg
      901 tcatctaggc atttgcgccg ggaagtgtgt agcatggcga aaaaacataa ctgctgaagc
      961 catgccactt tcctggaacg aaatcaaatc ccgcgccacc gccttctcca ggaactgggc
     1021 ggatgccggc aacgaagaca gccaggccaa gcctttcctg atcgacttct tcgaagtgtt
     1081 cggcatcacc gacaagcgcg tcgccagctt cgagcacgcg gtagcaaaac acggcggcgg
     1141 gcgtggcaaa gttgatttgt tctggcccgg catcctgctg gttgagatga aatcgcgcgg
     1201 caagaacctc gaccgcgcct tcgatcaggc catggactac ttccccggca tcgccgagcg
     1261 cgatctgccg cgctacgtgc tggtgtgcga ctttgcccgc ttccgcctgt acgacctcac
     1321 caccggcgac accagcgaat tcgcgctgaa agacctatac cagaacgccc gccagttcgg
     1381 cttcatcgcc ggctaccaga cgcaggtcat caagccgcag gaccccatca acatcaaggc
     1441 cgccgagcgc atgggacggc tgcacgacca gttgaaagcg gtgggctacg tcggccgccc
     1501 gctggaaatc tacctggtgc gcctgttgtt ctgcctgttt gcggaggaca ccggcatctt
     1561 cgacaagcgc cagttccagg actacgtcga aaaccgcacc ggcgaggacg gcagcgacct
     1621 cgcccaccac cttggcaccc tgttctacgt gctcaacacg ccccacgaca agcgcctgaa
     1681 aaacctggac gaagacctcg ccgccttccc ttatgtcaac ggccggctgt tcgaggaaat
     1741 gctgccgccg gccagcttcg acggcaaaat gcgcgatgcg ctgctggatt tgtgtgccct
     1801 cgactggagc ctgatttcgc ccgccatttt cggcagcctg ttccagagca tcatggacga
     1861 caaggcgcgc cgcaacctcg gcgcgcacta caccagcgag gaaaacatcc tcaagctcat
     1921 caagccgctg ttcctcgacg cgctgtgggc cgagttcgac aaggtcaagg gcaacaagaa
     1981 ccgcctgttc gaattccaca aaaaactacg caccctcacc ttcttcgacc ctgcctgcgg
     2041 ctgcggcaac tttctcgtca tcacctaccg cgaactgcgc ctgctggaac tggaagtgct
     2101 gcgcgccagc cgtgccagcg gccagcaggt gctggacgtg cagcagctca tcaacgtcga
     2161 cgtagaccag ttccacggca tcgagatcga ggaattcccc gcccagatcg cccaggtcgc
     2221 gctgtggctc accgaccacc agatgaacat gcgcgtgtcg gaagaattcg gcagctatta
     2281 cgcgcggatt ccgctcaaag ccacgccaca catcgtgcac ggcaacgcgc tgtggctgga
     2341 ctgggcggag gtgctggtgc cggaacggtg cgcctatgtg ctgggcaatc cgccattcgt
     2401 cggcgcaaaa ttcatggatg acgcccagcg cgaggacgcg cgcctggtct ttgccggcat
     2461 tgacaatgca ggcctgctgg attttgtcgc ctgctggtac gtcaaggcgg cgcggtatat
     2521 caacacccct cgccccacat cgtggggaga ggggccaggg gtgaggggcg aaccacttcg
     2581 tcgagacccc gctatcgaag aaaagctcaa gacctatgcc cgagaattac gtgcctcggc
     2641 cacggatgct gaacaaaagc tttggcatct tctgcgagac aggcgctttt ttgatttcaa
     2701 attccgccgc cagcatcctg tagcgggcta cattcttgat ttttattgcg ccgaggcaaa
     2761 atttgccatt gaactggatg gcggacaaca tgctcaggcg gaggaatacg atgcgcaaag
     2821 aacgcacacc ctcgcccagc aaggcatacg gctcttacgg ttctggaata acgagtttct
     2881 gcaaaacccc gaaggggtgc tggaaaccat tgcgacggct ttgcaagttc gcccctcacc
     2941 ctcgatccct ctccccgcta cgcagggcga gggaagcaga atccgttgcg ccttcgtctc
     3001 caccaacagc atcacgcagg gcgagcaggt cggtgcgctg tggggctggc tattgagcca
     3061 gggcgtccat atccacttcg cccaccgcac cttttcgtgg agcaacgagg cgcgcggcaa
     3121 ggccgccgtg cattgcgtca tcgtcggttt cggcagcggc gagcgcgccg acaagctgat
     3181 ctacgactac gacgacatca agggcgagcc gcacgcgatt ccggcgcgca atatcaatcc
     3241 ctatctggtg gatgcgccgg acatggtgct accgcgccgc tcgaagccca tttgcaacgt
     3301 gccggaaatc ggtatcggca acaagcccat agacggcggc cattacctgt tcacgccgga
     3361 ggaaaaggcg caattcctgc tgaaggagcc caccgcagcg ccgtatttcc ggcgctggat
     3421 aggcgccgac gagttcatca atggctacga gcgatggtgc ctgtggctgg gcgactgccc
     3481 gccgcaccaa ttgcgcgcca tgccggaagc catgaaacgg gtggatatgg ttcgccgatt
     3541 tcggcttggc gagggaataa ataccaaagg caaaaaggca gagaaggcgc ctcctgaatc
     3601 gaccagaaaa cttgcacaaa ctcctacgcg cttccacgtg gagaacatac cctttacccc
     3661 atacttggtt ttgcccgagg tttcatccga gcgccgcgcg ttcatcccga ttggctatga
     3721 gcagcccgaa acgctatcaa gcaatctggt cagaatcctg cctgatgcca ccctgttcca
     3781 cttcggcgtg ctgacttcca ccatgcacaa cgcctgggtg cgttccgtgt gcggccgtct
     3841 gaaaagtgac tttcgctatt ccataagcat cgtctacaac a
//
LOCUS       DAOMFA010000145         3852 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_3290_length_3852_cov_5.334738, whole genome shotgun sequence.
ACCESSION   DAOMFA010000145 DAOMFA010000000
VERSION     DAOMFA010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 3852)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3852)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3852
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3290_length_3852_cov_5.334738"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..652
                     /locus_tag="PKW44_07255"
                     /pseudo
     CDS             1..652
                     /locus_tag="PKW44_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829096.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-related protein"
     gene            680..1228
                     /locus_tag="PKW44_07260"
     CDS             680..1228
                     /locus_tag="PKW44_07260"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019896541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HQR51418.1"
                     /translation="MKTRAKHPSSRAAWLPKPAGSGAYRHWLVDAGSLTRRLQAHCKA
                     FSVQKVRQQRGRALRDEAQLLGMRAHENALLREVFLCCGDRPLVFAHSVLPRSSLRGA
                     WHGLGRLGPRPLGAALFANPVVVRTPLSYRKLAPSHALYRRAAELLNNKPPCLWARRS
                     VFTLRARSIMVTEVFLPGVLEL"
     gene            1225..2088
                     /gene="ubiA"
                     /locus_tag="PKW44_07265"
     CDS             1225..2088
                     /gene="ubiA"
                     /locus_tag="PKW44_07265"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013294569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HQR51419.1"
                     /translation="MNNWKERLNAYEKLMRLDKPIGILLLLWPTLWALWIAGHGRPDW
                     DVVWIFVLGTVLMRSAGCVINDYADRHIDPHVQRTRERPLAARTVSGREALLLAAFLC
                     LLAFGLALLLNRLTVMLSLAALFLAASYPFSKRFFALPQAYLGIAFGFGIPMAFAAQL
                     WHVPQLAWVLLLANVFWALAYDTEYAMVDRDDDIKLGIKSSAIFFGRYDVLAVMACYA
                     AMLSVLAWVGWKLHLKWPYHVGLGVAAGLAIYHYTLICGRERAGCFKAFMHNNWLGVA
                     VFAGLLLSYQA"
     gene            2171..2599
                     /gene="rplM"
                     /locus_tag="PKW44_07270"
     CDS             2171..2599
                     /gene="rplM"
                     /locus_tag="PKW44_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012777558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HQR51420.1"
                     /translation="MKTFSAKAHEVKRDWFVVDATDVVLGRLASAIAHRLRGKHKTIY
                     TPHVDTGDYIVVVNADKLKVTGDKAENKLYHRHSGYPGGIYTTNFGKMQQRFPGRALE
                     KAVKGMLPKGPLGYAMFRKLKVYAGPKHDHVAQQPQPLEI"
     gene            2609..3001
                     /gene="rpsI"
                     /locus_tag="PKW44_07275"
     CDS             2609..3001
                     /gene="rpsI"
                     /locus_tag="PKW44_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011310966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HQR51421.1"
                     /translation="MIGKYNYGTGRRKSSVARVFLKSGKGNIVINDKPADEYFSRVTS
                     RMILRQPLVLTNNESSFDIMVNVDGGGESGQAGAVRHGITRALIDYDAALKSALSHAG
                     LVTRDAREVERKKVGLRKARRAKQFSKR"
     gene            3161..>3852
                     /gene="argC"
                     /locus_tag="PKW44_07280"
     CDS             3161..>3852
                     /gene="argC"
                     /locus_tag="PKW44_07280"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HQR51422.1"
                     /translation="MQSKKIKAGIVGGTGYTGVELLRLLAVHSGVELQCITSRGEAGQ
                     LVSDLFPSLRGHVDMVFTDPAQANLATCDVVFFATPNGIAMQQARALLDAGVRVIDLA
                     ADFRIKDVAVWEKWYGMTHACPDLVGQAVYGLPEINREQIRNARLVANPGCYPTAVQL
                     GFLPLLEAGVVDSAGLIADAKSGVSGAGRKAETPILFAEAGDNFKAYGVGGQRPLSGI
                     IKGVAGVSGQKIG"
BASE COUNT          746 a         1090 c         1185 g          831 t
ORIGIN      
        1 cgtgtcctgc tggcaaaggc ggcaatctta ctgggtgtgc ccgtaaatcg aggaaagcga
       61 agcgctgcaa ctcaacaccg cgctggaaac ctacgaaaaa ctcaccgcag ccctgccaga
      121 actgaaagtc gggctgatgc atggccgcat gaagcccgcc gagaaacagg cagtaatgca
      181 ggcgtttacg gccaacgaag tgcaattgct ggtggcgacc acggtgatcg aggtcggtgt
      241 ggatgtgccc aatgccagcc tgatggtgat cgagcatgcc gaacgcatgg ggttgtcgca
      301 gctacaccag ttgcgcggcc gcgtcgggcg aggctcggca caaagcgcct gctttctgct
      361 ctatcagctc ccgctatccg aaactgcacg tgcccgactc aagatcatct acgagagcaa
      421 cgatggcttc gaaatctccc gcgccgatct gcatttgcgc ggtcccggtg agttccttgg
      481 tatccgtcaa agcggtgtgc cattactgag aatcgctgac ctggaacgcg atgccgatct
      541 gctggaagcc gcacgtcata tcgcagaaag cctactgcgt gaacagccgc aggcggcaga
      601 agcccacctg gcgcgttgga tgggacgggc aggagagtat gtcaaagtgt gatcgacgcc
      661 atatggcata atcagcgccg tgaaaacccg tgccaagcat ccttcctcgc gcgctgcatg
      721 gctgcccaag ccagccggca gcggcgctta ccggcactgg ctggtggacg cgggatcgct
      781 gacccggcgt ctgcaagcgc actgcaaggc gttttcagta caaaaagtgc gccaacagcg
      841 ggggcgtgca ctacgcgatg aagcacagtt gctgggtatg cgagcccatg aaaatgcgct
      901 gttgcgtgaa gtttttctgt gctgcggcga caggcctttg gtatttgcac acagcgtact
      961 gccccgtagc agcctgcgcg gcgcgtggca tgggctgggg cgcctggggc cacgcccgtt
     1021 gggcgcggca ctgttcgcca atccagtggt ggtgcgcacc ccgctcagtt atcgcaaatt
     1081 ggcgcccagc catgcgttat accggcgcgc agcagaactg ctgaataata agccgccatg
     1141 cctgtgggcg cgccgttccg tatttaccct gcgcgccagg agcattatgg tgacagaggt
     1201 ttttttgccc ggagtgcttg agctttgaac aactggaaag agcgactgaa tgcctacgag
     1261 aagctgatgc ggctggacaa gccgattggc attctgctgc tgctgtggcc tacgttgtgg
     1321 gcgctgtgga ttgccggcca tggccgtccg gattgggatg tggtctggat tttcgtgctc
     1381 ggcacggtat tgatgcgctc ggcgggttgc gtcatcaacg attacgccga ccgccatatc
     1441 gacccgcatg tgcaacgcac ccgggaacgt ccgctggccg cccggacggt gtccgggcgc
     1501 gaggcgctgc tgctggccgc cttcctttgc ctgctggcgt tcgggctggc gctgctgctc
     1561 aaccgcctca cggtgatgct gtcgctggcc gcgctgttcc tggccgccag ctatcctttc
     1621 agcaagcgct ttttcgcctt gccgcaggcc tatctcggca ttgcctttgg tttcggcatt
     1681 cccatggcgt tcgccgcgca gctctggcat gtaccgcagc tggcctgggt gctgctgctg
     1741 gccaacgtgt tctgggcgct ggcctacgat accgaatatg cgatggtgga ccgcgatgac
     1801 gatatcaagc ttggcatcaa gtcgtctgcc atcttcttcg gccgttacga cgtgctggcg
     1861 gtgatggcgt gttacgctgc gatgctgagt gttctggcct gggtcggctg gaagctgcat
     1921 ttgaagtggc cgtatcatgt cggcctggga gtcgctgccg gcctcgcgat ctaccattac
     1981 accctgattt gtggacgcga acgggcgggt tgtttcaagg cgttcatgca caacaactgg
     2041 ctgggggtag cggtatttgc cgggctgcta ttgagttatc aagcgtagtt ctcagatttc
     2101 gtttgacaac gcgcgtaacc ctggcataga atccgcctct ttcatttttc cggcgattac
     2161 ggtcaaaccg atgaaaacct tttctgcaaa agcgcatgaa gtgaagcgcg actggttcgt
     2221 ggtagatgcc acggacgtag tgctgggccg tctggcgagt gcgattgcac atcgtctgcg
     2281 tggcaagcac aagactattt acactccgca cgttgacacc ggcgattaca tcgtcgtggt
     2341 caacgctgac aagctcaagg ttaccggcga caaggccgag aacaagctgt atcaccgtca
     2401 cagtggttat ccgggcggta tttacaccac caatttcggc aagatgcaac agcgtttccc
     2461 gggccgtgcc ctggaaaaag cggtcaaggg catgttgccg aagggcccgc tgggttatgc
     2521 gatgttccgc aagctcaagg tgtatgccgg tcccaagcat gaccacgtgg cgcagcaacc
     2581 ccaacccctg gaaatttaag gatcaagcat gatcggtaaa tacaactacg gaaccggccg
     2641 ccgcaagagc tcggttgcgc gcgttttcct gaagtctggc aagggcaata tcgtcatcaa
     2701 cgacaagccg gccgacgaat acttctcccg cgtgacttct cgcatgatcc tgcgccaacc
     2761 gctggtactg accaacaacg agtccagctt cgatatcatg gtcaatgttg acggtggtgg
     2821 cgaatccggc caagccggcg ctgtgcgcca tggcattacc cgtgcgctga tcgattatga
     2881 cgctgcactc aagagcgccc tgtcccacgc cggtctggtg acccgcgatg cacgcgaagt
     2941 ggaacgcaag aaggtcggcc tgcgcaaggc gcgtcgcgcc aagcaattct ccaagcgcta
     3001 aacccttctg gttctgccgc aaaaagccgc ctagtgcggc tttttgcatt taagccgccc
     3061 ggaatttgag gcagtcacag tttttgttgc cttttggcag ggggctaatt aatatgggcc
     3121 gctcccgaat acgttaaatc catttatttt ttaaagagat atgcagtcca agaagataaa
     3181 agcaggcatt gtcggcggta ccggatatac cggcgtcgag ttgttgcgtc tactggccgt
     3241 tcattccggc gtggagctcc agtgcatcac ctcgcgcggc gaagccgggc aactggtcag
     3301 tgatcttttc cctagcctgc gcggtcatgt ggacatggtt ttcaccgacc ctgcccaagc
     3361 caatctggca acttgcgatg tggtgttctt tgccactccc aacggcattg ccatgcagca
     3421 ggcgcgcgcg ttgctcgatg ccggagtgcg tgtgatcgat cttgcagcgg atttccgcat
     3481 caaggatgtg gcggtgtggg aaaaatggta tggcatgacg catgcctgcc cggatctggt
     3541 cgggcaggca gtttacggcc tgccggaaat caaccgcgaa cagatcagga acgcacgtct
     3601 agtggccaat cctggctgct atccgacggc ggtgcagctc ggtttcctgc ctttgctgga
     3661 agcgggcgtg gtggattccg ccggcctgat cgctgatgcc aagtccggcg tcagtggcgc
     3721 cgggcgcaag gcggagaccc ctattctgtt tgccgaggcc ggtgacaatt tcaaggccta
     3781 cggcgtgggc gggcaacgcc ctttgtcggg aattattaaa ggggtggcgg gggtgtcggg
     3841 gcaaaaaatc gg
//
LOCUS       DAOMFA010000146         3771 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_3389_length_3771_cov_5.154736, whole genome shotgun sequence.
ACCESSION   DAOMFA010000146 DAOMFA010000000
VERSION     DAOMFA010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 3771)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3771)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3771
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3389_length_3771_cov_5.154736"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..358)
                     /locus_tag="PKW44_07285"
     CDS             complement(<1..358)
                     /locus_tag="PKW44_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF520 family protein"
                     /protein_id="HQR51423.1"
                     /translation="MPSFDITSEADMVALKNAIDVSARQITNRYDFKGTSANVELNEK
                     DKLITLYGDSDFQLDQIKDILFPALEKKEPDSSKRLDQQDVQKVSGNKVKQLMNIKDG
                     IDSDLAKRIVKIIKDSG"
     gene            complement(441..752)
                     /locus_tag="PKW44_07290"
     CDS             complement(441..752)
                     /locus_tag="PKW44_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HQR51424.1"
                     /translation="MAQFDNVSVKKKANVYFDGKCVSHTILMPNGTRCTVGVIFPSRL
                     TFNTGSPELMEINSGICKVRLKDEDAWHTYQTGDKFAVPGNSSFDIETVETLDYVCHF
                     E"
     gene            complement(856..>1893)
                     /gene="leuB"
                     /locus_tag="PKW44_07295"
     CDS             complement(856..>1893)
                     /gene="leuB"
                     /locus_tag="PKW44_07295"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019898788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HQR51425.1"
                     /translation="GIGPEIVAQAMRVLEVLNKEGLGMEFVEAPLGGQAYDAYGAPYP
                     EFTQKVCRDADAVLLGAVGGPQYDTLDRPLRPERGLLAIRKDLGLFANLRPAVLYPEL
                     ANASTLKPEVVAGLDIMIVRELTGDIYFGQPRGMRTNDKGEREGFNTMIYSESEVRRI
                     AHVAFGIAMKRKKKLCSVDKANVLETTEFWKEIVIDVAKEYPEVELTHMYVDNAAMQL
                     IRNPKQFDVIVTGNIFGDILSDEASMLTGSIGMLASASLNESKKGLYEPSHGSAPDIA
                     GKNLANPIATILSAAMMLRYTFDNEAAAARIENAVKKVLAAGYRTGDIYEAGMKKVSC
                     SEMGDQIVAAM"
     assembly_gap    1894..1916
                     /estimated_length=23
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1951..2589)
                     /gene="leuD"
                     /locus_tag="PKW44_07300"
     CDS             complement(1951..2589)
                     /gene="leuD"
                     /locus_tag="PKW44_07300"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018604660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HQR51426.1"
                     /translation="MKAFTQLNGLVCPLDRANVDTDAIIPNQFLKSIKRSGFGPYLFD
                     EWRYTNYGEPGMDCSMRPLNKDFVLNQPRYQGAQVLLARDNFGCGSSREHAPWAIEDF
                     GFRVIIAPSFADIFFSNCYKNGILPIVAPAEIVDHLFKECEATEGYKLNVDLPSQTVT
                     LPSGKSFSFDITPHRKHCLLNGLDEIGLTLQHADDIRAFETRHKAAQPWLFA"
     gene            complement(2599..>3771)
                     /gene="leuC"
                     /locus_tag="PKW44_07305"
     CDS             complement(2599..>3771)
                     /gene="leuC"
                     /locus_tag="PKW44_07305"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HQR51427.1"
                     /translation="ARLQVETLDANIKDFGALVYFPFKHANQGIIHVIGPENGTTLPG
                     MTVVCGDSHTSTHGAFGALAHGIGTSEVEHVMATQTLIAKKSKRMLVKVDGKLGKGVT
                     AKDVALAVIGRIGTAGGTGYAIEFGGEVIRALSMEGRMTICNMAIEAGARAGIIAPDE
                     KTSAYLKGRLHAPKGADWDKAVAYWSTLHSDPDAQFDAVVELAGSDIEPQVTWGTSPE
                     QVLPIGSRVPDPARESDATRRTSIENALKYMGLTANTPIEQIQIDKVFIGSCTNSRIE
                     DLRAAAAVAKGKKVAGNVKLAMVVPGSGVVKRQAEEEGLDRIFTEAGFEWREPGCSMC
                     LAMNADRLSPGERCASTSNRNFEGRQGPGGRTHLVSPEMAAAAAIAGHFVDVRNQI"
BASE COUNT          686 a         1112 c         1123 g          827 t
ORIGIN      
        1 ggccggagtc cttgatgatt ttcacgatcc gcttggccag gtcgctgtcg atgccatcct
       61 tgatgttcat caattgcttg accttgttgc cggacacctt ctgcacgtcc tgctggtcga
      121 gtcgcttgct gctgtcaggc tccttctttt ccagcgctgg gaacaggatg tccttgatct
      181 ggtcgagctg aaaatcggaa tcgccgtaaa gcgtaatcaa cttatccttt tcattcaatt
      241 cgacgttggc cgaagtcccc ttgaaatcgt agcggttggt gatttgccgg gccgagacat
      301 caatcgcgtt ctttagcgca accatatcgg cttcggaagt gatgtcaaaa gaaggcattt
      361 ttaactcctg aaatctttaa ctacagggaa cactggagtc cacagagaaa aatctagcac
      421 ttgattccca tgactaaaag ttactcaaag tggcagacgt agtccagggt ttcaaccgtt
      481 tcaatatcga aactggagtt gcccggcacg gcaaacttgt cccccgtctg gtaggtatgc
      541 catgcgtctt catccttgag gcgcacttta caaatgccgg aattgatttc catcagctcc
      601 ggcgagccgg tattgaaggt gagtctgctg ggaaaaatga cacctaccgt gcagcgcgtc
      661 ccattgggca tcagaatggt gtggctgaca cacttgccat caaaatagac gttggctttc
      721 tttttgacgc tgacattgtc gaactgagcc ataatgatcc ccatacagtt tattgttcaa
      781 gtctggatta tcccagagaa aagggcctcc cggcaacgcg ggaagccctt ttgctggcct
      841 tgggaacgcg agaatttaca tcgcggcgac gatttgatcg cccatctccg agcacgatac
      901 ttttttcatt ccagcctcgt agatgtcgcc agtacggtag cccgcggcca gcaccttctt
      961 cacggcattc tcgatgcgcg ctgccgccgc ctcgttatcg aaggtatagc gcagcatcat
     1021 cgcggcagaa agaatcgtcg caatcgggtt agccaggttc ttgccggcga tgtccggtgc
     1081 ggagccgtgc gagggttcat acaggccctt cttgctttcg ttcagcgagg ccgatgccag
     1141 catgccgatg gaaccggtaa gcatggatgc ctcgtcggac aggatgtcac cgaagatgtt
     1201 gccggtgacg atgacgtcga actgcttcgg gttgcggatc agctgcatgg cggcgttgtc
     1261 cacgtacatg tgcgtcagct cgacttccgg gtattccttg gcaacgtcga tgacgatttc
     1321 cttccagaat tcggtggttt ccagcacgtt ggccttgtcc acggagcaaa gtttcttctt
     1381 gcgcttcatg gcgatgccga acgcgacatg cgcgatgcgg cgcacttcgg attcgctgta
     1441 gatcatggtg ttgaagcctt cgcgctcgcc cttgtcgttg gtgcgcatgc cgcgcggctg
     1501 gccgaaatag atgtcgccgg tcagttcgcg cacgatcatg atatccagcc ccgccaccac
     1561 ttccggcttg agggtggatg cgttggccag ttccgggtag agcacggcag gccgcaggtt
     1621 ggcaaacagg ccgagatcct tgcggatggc cagcaggccg cgctcggggc gcagcggacg
     1681 atccagcgtg tcgtattgcg ggccgccgac ggcgcccagc agcacggcgt ccgcgtcacg
     1741 gcaaaccttc tgggtaaatt ccggataggg cgcgccgtag gcgtcatagg cctggccgcc
     1801 aagcggcgcc tcgacgaact ccatgccgag accttccttg ttcagtactt ccagcacgcg
     1861 catggcttgc gcgacgattt ccgggccgat gccnnnnnnn nnnnnnnnnn nnnnnntgcg
     1921 actttcatgt tgacttcctt tatctaaaag ttaagcgaaa agccagggct gcgcggcttt
     1981 gtgtctggtt tcaaaagcac ggatatcgtc cgcgtgttgc agcgtcaggc cgatctcgtc
     2041 gaggccgttg agcaggcaat gcttgcggtg cggcgtgatg tcgaagctga acgacttgcc
     2101 ggatggcaac gtcacggtct gcgacggcag gtccacgttc agcttgtagc cttcggtggc
     2161 ctcgcattcc ttgaacagat gatccacgat ttcagcaggc gccacgatgg gcaggatgcc
     2221 gttcttgtag cagttgctga agaaaatatc ggcgaacgac ggcgcgatga tgacgcggaa
     2281 accgaaatcc tcgatcgccc acggcgcgtg ttcacgcgaa gagccgcagc cgaagttgtc
     2341 gcgcgccagc agcacctgtg caccctggta gcgcggctgg ttgagcacga agtccttgtt
     2401 gagcggccgc atgctgcaat ccatgcccgg ctcgccgtag ttggtgtagc gccactcgtc
     2461 gaacaggtag gggccgaagc cggaacgctt gatggatttc aggaactgat tggggatgat
     2521 cgcgtcggta tccacgttgg cgcggtcgag cggacatacc aaaccgttta attgggtaaa
     2581 tgctttcatg gctgctcctt aaatctggtt gcgcacgtcg acgaaatggc cggcaatcgc
     2641 cgcggcggcg gccatttccg ggctcacgag gtgggtgcgc ccacccggcc cctgacggcc
     2701 ttcgaagttg cggttggagg tcgaggcgca gcgctcgccc gggctcagac ggtcagcgtt
     2761 catcgccaga cacatggaac atcccggctc gcgccactcg aagcccgcct cggtgaaaat
     2821 cctgtccagg ccctcttcct cggcctggcg cttgaccacg ccggaacccg gcaccaccat
     2881 cgccagcttg acgttgcccg cgaccttctt gcccttcgcc acagccgcgg cggcgcgcag
     2941 gtcttcgatg cgcgagttgg tgcaggagcc gatgaatacc ttgtcgatct ggatttgttc
     3001 aatcggcgta ttggctgtca ggcccatata cttgagcgcg ttctcgatgc tggtgcgcct
     3061 ggtggcgtcg ctttccctgg ccgggtcggg cacgcgcgaa ccgatgggca gcacctgctc
     3121 gggcgaagtg ccccacgtca cctgcggctc gatgtcggag ccagcaagtt ccaccaccgc
     3181 gtcgaattgc gcgtcgggat ccgaatgcag cgtgctccag taggccacgg ccttgtccca
     3241 gtccgccccc ttgggcgcgt gcagacggcc cttgaggtag gcagaagtct tctcgtccgg
     3301 ggcgatgatg ccggcgcggg cgcccgcttc gatcgccatg ttgcagatgg tcatgcggcc
     3361 ctccatggaa agcgcgcgaa tcacttcacc gccgaactcg atggcgtagc cggtgccgcc
     3421 cgccgttccg atccggccga tcaccgccag cgccacgtcc ttggcagtca cgcccttgcc
     3481 gagcttgcca tccaccttga ccagcatgcg cttcgacttc ttggcgatca gggtttgcgt
     3541 cgccatcaca tgctccactt ccgaggtgcc gatgccgtgc gccagcgcgc cgaaagcgcc
     3601 gtgggtggag gtgtggctgt cgccgcacac caccgtcatg ccgggcagcg tggtgccgtt
     3661 ttccgggccg atgacgtgga tgatgccctg gttggcgtgc ttgaagggga agtacaccag
     3721 cgcgccgaaa tccttgatgt tggcgtcgag cgtctccacc tgcaggcgcg c
//
LOCUS       DAOMFA010000147         3699 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_3469_length_3699_cov_4.827936, whole genome shotgun sequence.
ACCESSION   DAOMFA010000147 DAOMFA010000000
VERSION     DAOMFA010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 3699)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3699)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3699
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3469_length_3699_cov_4.827936"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            11..277
                     /locus_tag="PKW44_07310"
                     /pseudo
     CDS             11..277
                     /locus_tag="PKW44_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830540.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
     gene            complement(303..926)
                     /locus_tag="PKW44_07315"
     CDS             complement(303..926)
                     /locus_tag="PKW44_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA/Hda family protein"
                     /protein_id="HQR51428.1"
                     /translation="MKQLLLDIQPAPAPTLDNFVPGRNAEALHSLQQAATGLGEVRFI
                     FFWGAGGSGKSHLLAAAAELARRHGVDLVAADDVQKLSGEAQITLFNTCNRLRENGGV
                     LVASGDAAPMQMGLRDDLATRLSWGLVYQLHPLSDEEKARALKTHAAARGMKLPDEVV
                     DYCLCHLQRDLRSLMTTLDALDQWSLTAKRPVTLPLLRQLLQLPLEH"
     gene            1019..2068
                     /gene="purM"
                     /locus_tag="PKW44_07320"
     CDS             1019..2068
                     /gene="purM"
                     /locus_tag="PKW44_07320"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HQR51429.1"
                     /translation="MNPSEPTKTSISYRDAGVDIDAGDALVEDIKPFAKRTMRPEVLA
                     GIGGFGSLFEVPKKFKNPVLVSGTDGVGTKLKLAFQLNRHDTVGIDLVAMSVNDILVQ
                     GAEPLFFLDYFACGKLEVGTAAQVIKGIAQGCEQAGCALVGGETAEMPGMYPAGEYDL
                     AGFAVGCVEKDAIIDGSTIQEGDVVLGLASSGAHSNGYSLVRKLIDRSGIDLDSDFHG
                     QPFRDVVMAPTRIYVKPLLKLLEALPVKGMAHITGGGLSGNVPRVLPEHLTAEIQQSA
                     WTLPPLFQWLQAQGNVTHDEMHRTFNCGIGMVVIVAAADAERAQNLLQAEGETVYRIG
                     SIRKRAEGEAQTLVV"
     gene            2173..2856
                     /locus_tag="PKW44_07325"
     CDS             2173..2856
                     /locus_tag="PKW44_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HQR51430.1"
                     /translation="MHRLWITCLLFLATGALAAPQNFTVVYHVTRNGQPFADVTETYR
                     QDGGRYKLESITKGIGVYALFGLRRLTSEGEVTPEGLRPTRFEQQQGDDAKRLVTADF
                     DWAAGTLTMSAKGKTKTATLLPGTQDLASYAYQFMFVPPQGDDVEIPVTTGKKLRTYH
                     YRAEARDVAIDTPAGKYKTVHFVNATEGGDDKQLWLGNEVNHIPVRIIMQDDKGATLE
                     QVLTSLRAE"
     gene            2858..>3699
                     /locus_tag="PKW44_07330"
     CDS             2858..>3699
                     /locus_tag="PKW44_07330"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HQR51431.1"
                     /translation="MPRWLRGNWSRLFLALLLSLLAHLWLAGGLRFMMPEWDKADPIV
                     VRLVNPPAKPVPPPAVAAKKPLTARPKPQPLPPPSTEPLPDAPEETTPVAQDLRTGDE
                     RSLPLEAEETLPAAQEPPIANGQSPVLATQPYILPEEEKLPPPPRHVEIDFQVLRKGG
                     KAGVEHQKYQVADDGSYLLQSVIEPKGLLALALSDLVQKSTGTVTENGLRPSNYLYQY
                     GRNTDKAQKADFDWKTGMVTMGTRAKQRTAELREGAQDMLSFMYQFMFVPPLQEMQLA
                     ITNG"
BASE COUNT          755 a         1151 c         1148 g          645 t
ORIGIN      
        1 gtgagcggcc tcggccattg ttctggctgt tctcgttcat gggggccggg ctgggggcga
       61 gcgtttcctc gatgcattga agttgttcac gcggctggtc aacatcgggg atgtacgttc
      121 acttgccaca catccggctt ctaccacgca ccgccaactt tcggcaaagg aactggaaaa
      181 taccggcgtt tccgaagaaa ccgtgcgtct atgcgtaggc attgagcaca tcgacgacct
      241 gcgcgcggat ttggaacaag cgctcgccgt cacctgattg aagctgccgc cgacctgctg
      301 gctcaatgct ccagcggcag ctgcagcaac tgcctcagca gcggcagcgt caccggccgc
      361 ttggcggtca gcgaccattg gtccagtgcg tccagcgtgg tcatcagcga gcgcaggtcg
      421 cgttgcaggt ggcacaggca gtagtccacc acctcgtccg gcagcttcat gccgcgcgcg
      481 gcggcgtggg tcttgagggc cctagccttt tcctcgtccg acaacggatg cagctggtac
      541 accagccccc atgaaagccg ggtcgccagg tcatcgcgca accccatctg catgggcgcg
      601 gcatccccgc tggcgacgag aacgccaccg ttttcccgca ggcggttgca ggtgttgaac
      661 agcgtgatct gggcctctcc ggaaagcttt tgcacgtcgt ccgccgccac caggtcaacc
      721 ccatgccgcc gcgccagttc ggcggcagcc gccagcagat ggctcttgcc gctgccaccg
      781 gcgccccaga agaaaataaa tcgcacctcg ccaaggccgg ttgctgcctg ttgcaggctg
      841 tgcagagcct cggcgttgcg gccggggacg aagttgtcca gcgtcggcgc gggggcgggt
      901 tgtatatcca gcagcagttg tttcattcgg ttttgggctg gccgcgtttt ctcgtttcgc
      961 ggccatttcg ggtaaaattc agcctcgtac tctatcctta gcaaagcgag aactcaactt
     1021 gaacccgtcc gaacccacca agacttccat cagctaccgc gacgccgggg tcgacatcga
     1081 cgccggggat gcactggtag aggacatcaa gcctttcgcc aagcgcacca tgcgcccgga
     1141 agtgctggcc ggcatcggcg gcttcggctc cttgtttgaa gtaccgaaga agttcaagaa
     1201 cccggtgctg gtgtccggca ccgacggcgt aggtaccaag ctcaagctcg ccttccagct
     1261 caacaggcac gacaccgtcg gcatcgactt ggtggcgatg agcgtcaacg acatcctggt
     1321 gcagggtgca gaaccgctgt tcttccttga ctacttcgcc tgtggcaagc tggaagtcgg
     1381 cactgccgcg caggtcatca agggcatcgc acagggctgc gaacaggccg gctgcgcgct
     1441 ggtgggcggc gagacggcgg aaatgccggg catgtacccg gcgggagaat acgacctggc
     1501 gggcttcgcg gtgggctgtg tggaaaagga tgccatcatc gacggctcaa ctattcagga
     1561 aggcgacgtg gtgctggggc tggcctccag cggtgcgcat tccaacggct attcgctggt
     1621 acgcaagctg atcgaccggt ccggcatcga cctggatagc gacttccacg gccagccctt
     1681 ccgcgacgtg gtgatggcgc cgacgcgcat ctacgtcaaa ccgctgctca agctgctgga
     1741 ggcgctgccg gtcaagggca tggcgcacat caccggcggc gggctgagcg gcaacgtgcc
     1801 gcgcgtgcta ccggaacatt tgaccgccga aatccagcaa agcgcctgga ccctgccgcc
     1861 gctgttccag tggctgcagg cgcagggcaa cgtcacgcac gacgagatgc accgcacctt
     1921 caactgcggc atcggcatgg tggtgatcgt ggctgccgcg gatgccgagc gcgcacaaaa
     1981 cctgctgcaa gccgagggcg aaacggtgta tcgcatcggc agcattcgca aacgcgccga
     2041 gggcgaggcg cagacgctgg tagtttaaac tgttgcagcg cgagcatctc gtccggactg
     2101 aagtgactac cagcttgtaa ccgttcccga actggtgcgt tatccgactg tacccaaccg
     2161 aggaaaaccg tcatgcatcg cctttggatc acatgcctgc tatttcttgc caccggcgcg
     2221 ctggccgcgc cacagaactt caccgtcgtc tatcacgtca cccgtaacgg tcagccattc
     2281 gccgacgtta ccgaaaccta ccgccaggat ggtgggcgct acaagctgga aagcatcacc
     2341 aagggcatcg gggtctacgc gctgttcggt ctgcgccgcc tgacgagcga aggggaagtc
     2401 acgccggaag ggctgcgccc cacacgtttc gagcagcagc agggcgacga cgccaagcgg
     2461 ctggtgacag cggatttcga ctgggcggca ggcacgctga ccatgtccgc caaaggcaag
     2521 accaagaccg caaccctgct gcccggcacg caggacctgg ctagctacgc ctaccagttc
     2581 atgttcgtcc cgccgcaggg ggacgatgtg gaaatccccg tcaccaccgg caaaaagctg
     2641 cgcacctacc attacagggc cgaggcgcgc gacgtagcaa tagacacgcc tgcaggcaaa
     2701 tacaagacgg tacatttcgt taacgccacc gaaggcggag atgacaagca gttgtggctg
     2761 ggcaacgaag tgaaccatat cccggtacgt atcatcatgc aggatgacaa gggcgcgaca
     2821 ttggagcagg tgctgaccag cctgcgtgcg gagtagtatg ccgcgctggc tccgaggcaa
     2881 ctggagccgc ctgtttctgg cgctgctgtt gtcgttgctg gcgcacctgt ggctggcagg
     2941 tgggttgcgc ttcatgatgc cggagtggga taaggccgat cccatcgtgg tacggctggt
     3001 caatccgcct gccaagccag tgccacctcc cgccgtggcg gcaaaaaaac cactgacagc
     3061 caggccaaaa ccgcaaccat taccgccacc ctcgaccgag ccccttccgg atgcgccgga
     3121 ggagactacg ccagtggcgc aggatctgcg caccggagat gaacgatcac tgccacttga
     3181 agcggaggaa actctgccag cggcgcagga accgcccatc gctaatgggc aatctccggt
     3241 attggcgaca caaccttata tcctgcccga ggaggaaaaa ttgccgccac caccgcgcca
     3301 tgtcgagatt gatttccagg tcctgcgcaa gggaggaaag gctggcgtgg agcaccagaa
     3361 ataccaggtc gccgatgacg gcagctacct gctgcaaagc gtgatcgagc ccaaggggct
     3421 gctggcgctg gctttgtccg atctggtgca gaaaagcacg ggcacggtga ccgagaacgg
     3481 gctacggcca tcgaattatt tgtaccaata tggccgcaac accgacaagg cgcaaaaagc
     3541 ggatttcgac tggaagaccg ggatggtcac catggggacc cgcgccaaac aacgcacggc
     3601 cgaattgcgc gaaggcgcgc aggacatgct gagcttcatg taccagttca tgttcgtgcc
     3661 accgctgcag gaaatgcagc tggcaatcac caacggcaa
//
LOCUS       DAOMFA010000148         3695 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_3474_length_3695_cov_5.196154, whole genome shotgun sequence.
ACCESSION   DAOMFA010000148 DAOMFA010000000
VERSION     DAOMFA010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 3695)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3695)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3695
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3474_length_3695_cov_5.196154"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            5..406
                     /locus_tag="PKW44_07335"
     CDS             5..406
                     /locus_tag="PKW44_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HQR51432.1"
                     /translation="MERNIFFGGSLFFFLLFLALTFHTTQKLPKRDNSQNITAEVSRG
                     KKIWETRNCIGCHTLLGEGAYFAPELGNVYVRRGPDFIKAWIKGQPTGVPGRRQMPQF
                     NLTDQELDDIVAFLKYASEINTNKWPPNIEG"
     gene            435..1808
                     /locus_tag="PKW44_07340"
     CDS             435..1808
                     /locus_tag="PKW44_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019898678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HQR51433.1"
                     /translation="MQYQSQAVAKPYFIAAIGLFVGQILFGLIMGLQYVVGDFMFPEI
                     PFNVARMVHTNLLIVWLLFGFMGAAYYLVPEESETELFSPKLALTMFWIFLIAGALTI
                     VGYLAVPYATLARITGNDLLPTMGREFLEQPTITKLGIVVVALAFLFNIGMTVLKGRK
                     TAISLVLVLGLLGLAVFFLFSFYNPDNLVKDKYYWWWVVHLWVEGVWELILGAILAFV
                     LIKVTGVDREVVEKWLYVIITMTLITGIIGTGHHYYWIGAPGYWQVWGSVFSALEPIP
                     FFMLTIFAFNMVNKRRREHPNQAAVLWALGTGVMAFLGAGVWGFLHTLAPVNYYTHGT
                     QMTAAHGHMAFYGAYVMVVLTIISYAMPVLRGRAANSNKAQVVEMWSFWLMTIAMVFI
                     TLFLTGAGILQTWLQRMGDHPMSFMDTQAQVAIFYWMREGAGLVFLIGLLTYVSSFFI
                     KGETKAG"
     gene            1877..2470
                     /locus_tag="PKW44_07345"
     CDS             1877..2470
                     /locus_tag="PKW44_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3 family protein"
                     /protein_id="HQR51434.1"
                     /translation="MTTERILTQTIPEQDGPPGDLAIWIFILAEMLVFGVLFAVYAFT
                     RAHQVELFNASQLNLSRTAGAINTLVLITSSYFVVRAVSAIRQGLSRDCVRWLVAGWL
                     LGGVFVLVKLWEFRQKFAAGIGLDSNDFYMFYFTLTFFHFMHVLMGMIILAFVINKAR
                     RGGYNAQDYVGVETGASFWHMVDLLWIILFPLVYVIR"
     gene            2478..2747
                     /locus_tag="PKW44_07350"
     CDS             2478..2747
                     /locus_tag="PKW44_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HQR51435.1"
                     /translation="MHFQSRRFSTMIWLLLVGLTLATLGIGEAGLAGKGVMLALLAIA
                     LIKGQMVANYFMGLRYAGWLWRGIILAYFLLVGGMIAIAYLVGLK"
     gene            2744..3553
                     /locus_tag="PKW44_07355"
     CDS             2744..3553
                     /locus_tag="PKW44_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbbQ/NirQ/NorQ/GpvN family protein"
                     /protein_id="HQR51436.1"
                     /translation="MNSVFGGDTMEHLPFYQATDDECELFERAWRNRLPLLIKGPTGC
                     GKTRFVSHMAARLKLPLYTVACHDDLTAADLVGRHLIGDGQTIWSDGPLTRAVREGGI
                     CYLDEVVEARKDTTVVLHPLADDRRMLPIDRTGELLHAPPSFMLVVSYNPGYQNFLKG
                     MKPSTRQRFVSLSFDFPDPAHEEAILVGETGVDAMVAKRLVGIANAFRTLRDHDLEEV
                     ASTRLLVYAATLIKDGCDPLRACRAALVETLTDDLEIVEALNEVVSATFGR"
BASE COUNT          703 a         1024 c         1096 g          872 t
ORIGIN      
        1 atccatggaa cgcaacatct ttttcggtgg gtcgttgttt tttttcctac tgtttcttgc
       61 gctgacattc cacaccacgc agaagctgcc aaaacgcgac aatagtcaga acatcaccgc
      121 tgaagtctcg cgcggcaaga aaatctggga aacgcgcaac tgcatcggct gccatacgct
      181 gctgggtgaa ggtgcctatt ttgcgccgga acttggcaat gtctacgtgc gccgcgggcc
      241 ggatttcatc aaggcctgga tcaaaggcca gccgaccgga gttcccgggc gccgccagat
      301 gccacagttc aacctgactg accaggaact cgacgatatc gtggctttcc tgaagtacgc
      361 ttcggaaatc aataccaaca agtggccgcc gaacatcgaa ggctaaccgt cacaattacc
      421 gggagatcaa gaccatgcaa tatcagtctc aggctgtggc gaagccttat ttcatcgccg
      481 ccatcggcct tttcgtcgga cagatcctgt tcggcctcat catgggcctg caatacgtgg
      541 tgggggattt catgttcccc gaaattccat tcaacgtcgc gcgcatggtg cataccaacc
      601 tgctgatcgt gtggctgctg ttcggcttca tgggcgcggc ctattacctg gtgccggagg
      661 aatccgaaac cgagctgttc agtcccaagc tggcactaac catgttctgg atattcctca
      721 ttgccggtgc tctgaccatc gtcggctacc tggcagtgcc gtacgcgacg ctggccagga
      781 tcacaggaaa cgacctgttg ccgaccatgg ggcgtgagtt cctagagcag ccgacgatta
      841 ccaaactggg catcgtggtg gtggcgctgg cgttcctgtt caacatcggc atgactgtgc
      901 tcaaggggcg caaaacggcg atcagtctgg tgctcgtgct tggcttgttg gggttggcag
      961 tgttcttcct gttctcgttc tataacccgg acaatctggt gaaagacaag tactactggt
     1021 ggtgggtcgt gcacctctgg gtggaaggcg tatgggagtt gatcctcggc gctatcctgg
     1081 ctttcgtttt gattaaggtg accggcgtgg atcgcgaagt ggtggaaaag tggctttatg
     1141 tgatcatcac tatgacgttg atcaccggca tcatcggcac cggccaccat tactactgga
     1201 tcggtgcgcc ggggtactgg caggtgtggg ggagcgtgtt ctccgcattg gaacccatcc
     1261 cgttcttcat gctgacgatt tttgccttca atatggtgaa caagcgccgg cgcgagcatc
     1321 ccaaccaggc ggccgtgctg tgggcgctcg gtactggcgt gatggctttc ctcggtgcgg
     1381 gggtatgggg cttcctgcat acgctggcac ccgtcaacta ctatacccac ggcacgcaga
     1441 tgaccgcggc tcacggccac atggcgttct atggtgccta cgtaatggta gtgttgacca
     1501 tcatttccta tgccatgccg gtgctgcgcg gtcgtgcagc caacagcaac aaggcgcagg
     1561 tagtggaaat gtggtcattc tggctgatga cgattgccat ggtttttatc acgctgttcc
     1621 tcactggtgc gggtatcctg caaacatggc tacagcgcat gggtgatcat ccgatgagtt
     1681 ttatggatac gcaggcccaa gtcgccattt tctactggat gcgcgaaggt gccgggctgg
     1741 tgttcctgat cggattgttg acttatgtgt ccagcttctt catcaagggc gaaacaaaag
     1801 ccggctgagc cagcttgacc actgcatgga aagagaaggg ggccgtgccc ccttctttgt
     1861 tttcaatgcc aaccagatga ccactgaacg aattctgacg cagaccatcc cggagcagga
     1921 tggtcctccg ggcgatctcg ccatctggat tttcatcctg gccgagatgc tggtgttcgg
     1981 cgtgctgttc gcggtctacg cattcacccg cgcacatcag gtcgaactgt tcaatgcttc
     2041 gcaactcaac ttgagccgaa cagccggtgc gatcaacacg ctggtgctca tcaccagcag
     2101 ctactttgtc gttcgtgcag tctcggcgat cagacagggg ctgtcgcgtg actgtgtccg
     2161 ctggctggtc gctggctggt tgctaggtgg agtgttcgtg ctcgtcaaac tgtgggagtt
     2221 ccggcaaaaa ttcgccgctg gcatcggact cgacagcaat gatttttaca tgttctattt
     2281 cacactgacg ttctttcatt tcatgcacgt gctgatgggc atgatcatcc tcgcattcgt
     2341 catcaacaag gcgcggcgcg gcggctacaa cgcgcaggat tatgtcggag tggaaaccgg
     2401 tgcctcgttc tggcacatgg tcgatctgct ctggatcatc ctattcccgc tggtctacgt
     2461 gatacgctga ggtgagcatg cactttcaat ctcgccgctt ttccacgatg atttggctgc
     2521 tgttggttgg gctcaccttg gccacgttgg ggatcggcga agccggcctg gcgggcaaag
     2581 gagtcatgct ggcgctactg gccatcgcgc tgatcaaggg gcagatggta gccaattatt
     2641 tcatgggcct gcgttatgcc ggctggctgt ggcgcggcat catcctggct tattttctac
     2701 tggtcggcgg catgatcgcc attgcctatc tggtcggact aaaatgaatt cggtttttgg
     2761 aggagatacg atggagcatc tgccttttta tcaggcgacg gacgatgagt gcgaactgtt
     2821 cgagcgcgcc tggcgcaacc ggttgccgct actcatcaaa gggccgaccg gctgcggcaa
     2881 gacgcgcttc gtctcgcaca tggcggcgcg gctcaagctg ccgctttaca ccgtggcttg
     2941 ccacgacgat ctcacggccg ccgatctggt tggccgccac ctgatcggag acggccagac
     3001 catctggagt gatggcccgt tgacccgagc cgtcagggag ggcggcatct gctatctcga
     3061 cgaagtggtc gaggcgcgca aggatacgac ggtcgtattg cacccactgg cagacgacag
     3121 gcgcatgctg ccgatcgacc gtaccggcga actgctgcat gcgccgccca gcttcatgct
     3181 ggtggtatcg tacaaccccg gctaccagaa ttttctcaag ggcatgaaac cttcgacacg
     3241 gcagcggttc gtctcgttgt cgtttgattt ccccgatccc gcccacgaag aggccattct
     3301 ggtcggcgaa accggcgtcg atgcgatggt cgccaagcgg ctggtgggga ttgccaatgc
     3361 tttccgtacc ctgcgcgacc atgacctgga agaagtcgcc agcacccggc tgctggtcta
     3421 cgccgcgacg ctcatcaagg atggttgcga tccgttgcgt gcatgtcggg cggcgctggt
     3481 ggaaacactg actgacgacc tggaaattgt cgaggcgctg aatgaggtcg tgagcgccac
     3541 gttcggccgc tgatttctcc ccatggaaga atacattggc gaactttggc accgcttcat
     3601 cacacgggcg gccgaacagc ggtatcccga tgcggcggtc gatctggagg atattgccaa
     3661 ggctgctgcc atcttttttc gtgcgctggg cggcg
//
LOCUS       DAOMFA010000149         3685 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_3492_length_3685_cov_4.459229, whole genome shotgun sequence.
ACCESSION   DAOMFA010000149 DAOMFA010000000
VERSION     DAOMFA010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 3685)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3685)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3685
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3492_length_3685_cov_4.459229"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..358)
                     /gene="rpsB"
                     /locus_tag="PKW44_07360"
     CDS             complement(<1..358)
                     /gene="rpsB"
                     /locus_tag="PKW44_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HQR51437.1"
                     /translation="MSVTMRQMLEAGVHFGHQTRYWNPRMAPYIFGDRNKIHIVNLEK
                     TLPLFEDAMKYVRQLAANKGRILFVGTKRQARDIVKEEAARAGCAYVNHRWLGGMLTN
                     FKTVKQSIKRLTAYQTM"
     gene            complement(593..1996)
                     /locus_tag="PKW44_07365"
     CDS             complement(593..1996)
                     /locus_tag="PKW44_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HQR51438.1"
                     /translation="MIRKLLVVSAFLLVFAAPSFAKELPDFTELYEKQGPAVVNISTT
                     QTVHGGGPGMMPFPNVPEDDPFYEFFRRFSPPGGQGQQDYQTQSLGSGFIISNDGYIL
                     TNAHVVKDAEEVIVKLADKREFKARIVGIDRRSDVSLLKIDATGLPKVTLGDPGQLKV
                     GEWVVAIGSPFGLENTVTAGIVSAKGRALPQENYVPFIQTDVAINPGNSGGPLFNLKG
                     EVVGINSQIYSRSGGYMGLSFAIPIDVAMDVQNQLKAGGKVARGWLGISIQEITKELA
                     ESFGMKNTNGALVAGVEKGSPAEKAGLEAGDVILKFDGKVITSSSDLPRVVGATKPGK
                     DATIEILRRGSSKNVSVTVGELPSDDKEEAQPSRGPAKAEPNRIGLALRELTPQQKKK
                     LNGKNGLLVVGAEGSAAQAGIRRGDVILAVNNTEVQGLEQFNKQIAAVQSGKTVALLV
                     LRENSTLYVPVKVNGSK"
     gene            complement(1997..2476)
                     /locus_tag="PKW44_07370"
     CDS             complement(1997..2476)
                     /locus_tag="PKW44_07370"
                     /inference="COORDINATES: protein motif:HMM:NF016160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HQR51439.1"
                     /translation="MIEEQAIVVGIERDQALLEIVRNKPCGLCGQTRGCGISIWGRLL
                     GHRNSVFRAANQINARVGDSVVVGVDEKALLAGSLAVYGAPLFLLLAGAGMGSYLAPP
                     QGSVDAYALVGAFAGLVIALLWLRAHAAGRAPDARFRPVILRPTAASATLQVCQRGE"
     gene            complement(2482..2937)
                     /locus_tag="PKW44_07375"
     CDS             complement(2482..2937)
                     /locus_tag="PKW44_07375"
                     /inference="COORDINATES: protein motif:HMM:NF028497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucB/RseB C-terminal domain-containing protein"
                     /protein_id="HQR51440.1"
                     /translation="MLNEQGDVLEQVAFNQLMLIGSDDLNWFRPQIEHGKMYEMQPEE
                     TVTPASVEGDVWIVAQLPTGYRKVDQVRRTIPGKPHPVNHLVFSDGLASVSLFIEALA
                     RNAVPKQGHMVQGATNLYASAQDGYQVIVVGEVPEATVKQIAAAVSFKK"
     gene            complement(2962..3450)
                     /locus_tag="PKW44_07380"
     CDS             complement(2962..3450)
                     /locus_tag="PKW44_07380"
                     /inference="COORDINATES: protein motif:HMM:NF015825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB
                     domain-containing protein"
                     /protein_id="HQR51441.1"
                     /translation="MRGLLACLCTLPLFGQAAGKEAVDDPWLMLEKAGQAAHKLSYKG
                     IFVYQSGPIVSSMQIIHMNYAQGEFARLVSLDGAPREVLRQGNDVLIYNQQKEKVMIE
                     KRRVQNSFPALLPGLVDAALKANYQARSAGMERVGGREVQIIHLVPRDRYRYGCHFSV
                     DR"
     gene            complement(3478..>3685)
                     /locus_tag="PKW44_07385"
     CDS             complement(3478..>3685)
                     /locus_tag="PKW44_07385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR51442.1"
                     /translation="ASVAAVLFVGWMVLQKQAQSIPAAPSLAQNNVSAESVQSYLLAH
                     HEQSPENVMQTAYMRPVAYSGSGE"
BASE COUNT          787 a         1142 c          987 g          769 t
ORIGIN      
        1 tcatggtctg gtaagcggtc aggcgcttga tagactgctt gacagtcttg aagttggtca
       61 gcatgccgcc cagccagcga tggttaacgt aggcgcaacc ggcacgggct gcttcttcct
      121 tgacgatatc gcgcgcctgg cgcttggtgc cgacgaacag aatacggccc ttgttggcgg
      181 ccagctggcg cacgtacttc atggcgtctt cgaacagcgg cagggttttt tcgaggttga
      241 cgatatggat cttgttgcgg tcgccgaaga tatacggcgc catacgggga ttccagtaac
      301 gggtctggtg gccgaagtgc accccggctt ccagcatttg acgcatggtc acggacatga
      361 ttttctcctg taagggttag ttgcttccac gcgcccaaaa cacacccttt cgggcaccct
      421 gtacggagcg cgtgtgcaga ttagccgttc ataattaaac gggcggcgta tcctaccaca
      481 aacacccgcg cccattcaag gcataccgtc ggaccaggcg agtttacacg gattcgtcac
      541 gcacaaaaaa ggggcgcagc gcaccccttt tttccctcat cctggtttcc agctatttgc
      601 tgccgttcac cttgaccggc acatagagtg tgctgttctc acgcagaacc agcagcgcca
      661 cggtcttgcc tgattgtact gcggcgatct gcttgttgaa ctgctccaag ccctgcactt
      721 cggtattgtt gacggcaagt atcacatcgc cgcggcggat gccagcctga gcagcagacc
      781 cttcggcgcc aaccaccagc agaccgttct tgccgttgag ctttttcttc tgctgcgggg
      841 taagttcgcg cagcgccagg cctatgcggt tgggttcggc cttggccgga ccacgcgagg
      901 gctgggcttc ttccttgtcg tccgaaggca gttcaccgac ggtcacgctc acattcttgc
      961 tgctaccgcg acgcaggatt tcaatcgtgg catccttgcc cggcttggtc gcgccgacta
     1021 cgcgcggcag gtcagaagac gaggtgatta ccttgccatc gaacttgaga atgacatcgc
     1081 ccgcctccag cccggccttt tccgccggac tgcctttttc cacaccagcc accagcgcac
     1141 cattggtgtt tttcatgccg aacgattcgg ccaattcctt ggtaatctcc tggatactga
     1201 tgcccagcca gccacgcgcg accttgccgc cggccttgag ctgattctgc acatccatgg
     1261 cgacgtcgat cggaatagcg aacgataacc ccatatagcc gccggagcgg ctgtaaatct
     1321 gcgagttgat gccaaccact tcacccttga gattgaacag cggaccgccg gaattgccgg
     1381 gattgatggc cacgtcggtt tggatgaacg gcacgtagtt ttcctgcggc agggcgcgcc
     1441 ccttagcgct gacgatgccg gcagtgacgg tgttttccag tccgaacggc gagccgatcg
     1501 ccaccaccca ttcacccacc ttgagctggc cggggtcgcc caatgtcacc ttgggcagtc
     1561 ccgtggcgtc aatcttgagc agagaaacgt cactacggcg gtctatgccg acgatcctgg
     1621 ccttgaactc gcgcttgtcg gcaagcttga cgatcacctc ctcggcatcc ttgaccacgt
     1681 gtgcattagt caggatatag ccgtcattgc tgatgataaa gcctgaacca agcgactgcg
     1741 tctggtagtc ctgctgcccc tgtccgcccg gcggggagaa acggcggaaa aactcgtaaa
     1801 acgggtcgtc ttccggcacg ttggggaacg gcatcattcc cggcccgccg ccatgcacgg
     1861 tctgcgtggt actgatattg accacggccg gcccctgctt ctcataaagc tcggtaaagt
     1921 caggcagctc cttggcaaaa gagggcgctg caaataccag caaaaaggcg gagaccacca
     1981 gcaattttct aatcatttat tcacctcgtt gacatacttg tagagtagca ctggccgcag
     2041 tggggcgcag gatcaccggc cggaagcggg cgtccggcgc gcgtccggca gcgtgtgcac
     2101 gcagccacag cagagcgatg accaatccgg caaaggcccc aaccagcgca taagcgtcca
     2161 cactgccctg ggggggcgca agataactgc ccatgcccgc cccggccagc agcaaaaata
     2221 aaggagctcc ataaacggcc agcgatccgg ccagcaacgc cttctcatcg acgccgacga
     2281 ccacactatc gccgacccgt gcgtttattt gattagcagc ccggaacacg ctgttacggt
     2341 gtccgagcaa tctcccccag atggatatgc cgcagccgcg cgtctgaccg cacaacccgc
     2401 aaggcttgtt gcgcacaatc tccagcaatg cctgatcgcg ctcgatgcca acgacaatgg
     2461 cttgttcttc tatcatgacg actacttctt gaagctgaca gcggctgcaa tctgcttgac
     2521 ggtggcttcg ggcacctcac ccaccacgat gacctggtaa ccatcctggg cgctggcata
     2581 aaggtttgtc gccccttgca ccatatgccc ctgcttggga acagcattcc gtgccagcgc
     2641 ctctatgaac aacgacaccg aggccagccc atcggaaaaa accaggtgat tgactggatg
     2701 gggcttgccg ggaatagtgc ggcgcacctg atcgaccttg cgatagccgg tggggagctg
     2761 cgccactatc cacacgtcac cctctacaga ggccggcgtt accgtctcct ccggctgcat
     2821 ttcgtacatc ttgccatgtt cgatctgggg gcggaaccaa ttcaggtcat cgctgccgat
     2881 caacatcaac tggttgaaag ccacctgttc gagcacatcg ccctgttcgt tcaacatgat
     2941 cgatttcagc agtaatccgg actagcgatc caccgagaag tggcagccat aacggtatcg
     3001 atcgcgtgga accaaatgaa tgatctgcac ctcacgccca cccactcgct ccatccctgc
     3061 cgagcgggcc tgataattgg ccttgagggc ggcatccacc aagcccggaa gcagcgcggg
     3121 gaaagagttc tgcaccctgc gtttctcgat catcactttt tctttttgct gattgtatat
     3181 cagcacatca ttaccctggc gcagcacttc acgcggcgcc ccgtcgagcg acaccagacg
     3241 cgcaaattca ccctgtgcat agttcatatg gataatctgc atggaactga cgatgggccc
     3301 tgactgataa acaaaaatac ccttgtagct caatttgtgc gcagcctggc cggctttctc
     3361 caacatcagc cacggatcat caaccgcctc ttttccggca gcctgcccga acaaaggtag
     3421 cgtgcataaa caagccagca ggccgcgcaa caacggcttg gataaaacaa ccatgcgcta
     3481 ctctccgcta ccagaatagg cgacaggacg catataagca gtctgcatga cgttctcggg
     3541 tgattgctca tgatgcgcca gcaaataaga ctggacggac tcggctgaaa cattgttctg
     3601 agccaaagag ggagctgccg gaatggattg cgcttgcttc tgcaagacca tccatccgac
     3661 aaacagaacc gcggcaacag aagcc
//
LOCUS       DAOMFA010000150         3660 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_3538_length_3660_cov_4.656033, whole genome shotgun sequence.
ACCESSION   DAOMFA010000150 DAOMFA010000000
VERSION     DAOMFA010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 3660)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3660)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3660
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3538_length_3660_cov_4.656033"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..677
                     /locus_tag="PKW44_07390"
     CDS             <1..677
                     /locus_tag="PKW44_07390"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HQR51443.1"
                     /translation="PLIGLMPHATLAAVVIVYSIGLIQPAEFRAIRGVRKMEFHWALV
                     ACAGVLLFGTLKGIVVAIIVSMISLANQTAHPRVSVIGRKRGTNVLRPLSPEHPDDET
                     FPGLLILRPEGRLFFVNAQNVADQIHALIEQHHPRVLALDLSGVTDIEYSALQMLIES
                     EQRAAEAGIERWLVGLNPGVLEVVRHAGFDQRLGRERMLFDAHKAIERYQEQKADGAA
                     ALPEAG"
     gene            691..1017
                     /locus_tag="PKW44_07395"
     CDS             691..1017
                     /locus_tag="PKW44_07395"
                     /inference="COORDINATES: protein motif:HMM:NF019500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HQR51444.1"
                     /translation="MSLEFNIKFRRALFSGFAAVWSVFASLLCMMAVLGLAVAAIEGW
                     SSGDGLYFAFVSGLTIGYGDLVPTATLARVLAILIGFLGILLGGLVAAVGVHALTTAL
                     SETRHD"
     gene            1010..1135
                     /locus_tag="PKW44_07400"
     CDS             1010..1135
                     /locus_tag="PKW44_07400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR51445.1"
                     /translation="MTSPQGTGVLRLDIVAGLVAAAVEAVFSAHTRPRWGMLGIE"
     gene            complement(1429..1908)
                     /locus_tag="PKW44_07405"
     CDS             complement(1429..1908)
                     /locus_tag="PKW44_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019897808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HQR51446.1"
                     /translation="MHIAMNTVVSITYELRDSDGNILEAAADPVTYLHGGYDNIFPRV
                     EEELHGKKVGDSIDLTLEPVDAFGDYDEELVQIEPVSAFPSDKLEVGMQFEGEDPSGD
                     VVLYTITDIGDGKAVVDGNHPWAGERVNFKCTVTNIRSATKEEINHGHVHGAGGHHH"
     gene            complement(1911..2375)
                     /locus_tag="PKW44_07410"
     CDS             complement(1911..2375)
                     /locus_tag="PKW44_07410"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HQR51447.1"
                     /translation="MLKPGLSAPDFTLPDADMELVKLSRLKGKTIVLYFYPKDDTPGC
                     TAQAIEFSDLEEGFARCNALVIGISRDDCISHADFRDKHGLAVNLLSDVEGKVCEKYG
                     VWQEKEKDGIKKMCILRSIFVIDPEGVLRHTFYGANPKGHAGEILNLIKEMS"
     gene            2450..3562
                     /locus_tag="PKW44_07415"
     CDS             2450..3562
                     /locus_tag="PKW44_07415"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HQR51448.1"
                     /translation="MKIPFLGGLTASEFVRDYWQKKPLLVRNAFPGFGDMISPDELAG
                     LACEEDAQSRLVLHHRGKWKLEPGPFDEERFANLPDKGWSLLVQGVNHFLPEADELLH
                     RFDFIPRTRLDDLMVSLAPDGDGVGPHFDSYDVFLLQGRGKRLWRVSSQQDHELVKDA
                     PLRILKNFETNEEWLLEPGDMLYLPPRLAHWGIAVGECMTWSVGFRAPTAQELSTQFL
                     AYLQDHLCVEGMYADPDLQPQQHPAQIGEQMLGKVQNMLQEIRWNDHDVTRFLGSYLT
                     EPKPHIVFDRPPRMGLDRFREKLARHGIRLDLKSQMLFHGALMFINGESVEANSATLA
                     CLTTLADHRRLPPSPGLPEPAIGLLHQWHAAGYLGF"
BASE COUNT          669 a         1075 c         1146 g          770 t
ORIGIN      
        1 caccgctgat cggactgatg ccgcacgcca cgctggcggc ggtcgtcatc gtctactcca
       61 tcgggctgat ccagccggcg gagttccgcg ccatccgcgg cgtgcgcaag atggagttcc
      121 attgggcgct ggtcgcctgt gccggcgtgc tgctgttcgg cacgctcaag ggcatcgtgg
      181 tggccatcat cgtgtcgatg atcagtctgg ccaaccagac cgcccacccg cgcgtctcgg
      241 tcattggccg caagcgtggt actaacgtgc tgcgaccgct gtcacccgaa caccccgacg
      301 acgagacctt cccgggcctg ctgatcctgc ggcccgaggg gcggctgttc ttcgtcaatg
      361 cgcagaacgt cgccgaccag atccacgcgc tgatcgagca gcaccatccg cgcgtgctgg
      421 cgctcgacct gagcggggtt accgatatcg aatactcggc gctgcagatg ctgatcgaaa
      481 gcgagcagcg cgccgccgag gccggcatcg agcgctggct ggtcgggctg aacccgggcg
      541 tgctggaagt ggtgcgccac gccgggttcg accagcggct cggccgcgag cggatgctgt
      601 tcgatgcgca caaagcgatc gagcgctatc aggaacagaa ggcggacggt gctgcggcac
      661 tgccggaagc aggctgagag ggccgccgcg atgtcgctgg agtttaatat aaagttcagg
      721 cgcgcgctct tcagcgggtt tgccgccgtc tggtccgtgt tcgcgagcct gctctgcatg
      781 atggctgtcc tcggcctggc agtcgccgcc atcgagggct ggtcgtccgg tgacggcctt
      841 tacttcgcct tcgtctcggg gctgacgatc ggctacggcg acctcgtgcc cacggcgacc
      901 ctggcccggg tgctggcgat cttgatcgga tttctcggca tcctgttggg tgggctggtg
      961 gccgccgttg gcgtgcacgc gctgacgacc gcgttgagcg agacgcgtca tgactagccc
     1021 gcaaggcacc ggcgtactgc gcctcgacat cgtcgccggt ctcgttgccg cggccgtcga
     1081 agcggtgttc tcggcacaca cacggccgcg ctggggcatg ctggggattg aatgacagct
     1141 ttaccgctag gttgccgtta gagttttgcc ttaggcccag cggtagcagt gggcgaagag
     1201 cacgaattgg ctgaccggca ggtttccgag ttgagcagcc ctttgaatgt ccgcactgga
     1261 gtgcgtggcg gccgtcaggt tgtggccgat agctgacatt catgactcag aaggaaacaa
     1321 ggaaactgcg tattcccagt gcccagattt atcgtctcgg gcgcgccaaa taaaaaagcg
     1381 gttagatgca gatgcgtcta accgcttttt ctttgcctgt agcaggaact agtggtggtg
     1441 cccacccgcg ccgtgcacgt ggccgtggtt gatttcttcc ttggtggcag agcggatatt
     1501 ggtgacggtg catttgaagt tgacgcgctc tccggcccag gggtggttgc cgtctacgac
     1561 cgccttgccg tcgccgatgt cggtgatggt gtagagcacg acatcgccac ttgggtcttc
     1621 accctcaaac tgcatgccga cttccagttt gtcggaaggg aaggcgctga caggctcgat
     1681 ttgcaccagt tcttcgtcgt agtcaccgaa cgcgtcgacg ggctccagcg tcagatcgat
     1741 gctgtcccct actttcttgc cgtgcagttc ctcctcgaca cgcgggaaga tgttgtcgta
     1801 gccgccatgc agataggtca cggggtctgc ggcggcttcc agaatgttgc cgtcgctatc
     1861 gcgcagttcg taagtgatgg agactacagt gttcatggca atatgcatgg ttaactcatt
     1921 tcctttatca ggttcaaaat ttccccggca tgccccttgg ggttcgcacc gtagaaggta
     1981 tggcgcaaga cgccttccgg gtcgatgacg aagatggagc ggagaatgca cattttcttg
     2041 atgccgtcct tttccttctc ctgccatacg ccgtactttt cgcacacctt gccttcgacg
     2101 tccgacagca ggttgacggc aaggccgtgc ttgtcacgga agtccgcgtg gctgatgcaa
     2161 tcgtcacgac tgataccgat gaccagtgcg ttacatctgg caaaaccctc ctccaggtcg
     2221 ctgaattcga tggcttgggc cgtgcaaccg ggggtgtcat ccttggggta aaaatacaat
     2281 acgatggttt tgccctttaa cctggatagt ttgaccagtt ccatgtcagc atcgggcagg
     2341 gtgaagtcag gcgcggacaa tccgggtttc agcatggcgt ctttcagaag ggctgccgtt
     2401 taattctaaa ccatttccag ccacttcacg caagcgttat gattgccaca tgaagattcc
     2461 ttttctcggt ggattgaccg ccagcgagtt tgtgcgcgac tactggcaaa agaaacccct
     2521 gctagtacgt aacgccttcc cggggtttgg cgacatgatt tcgccggacg aactggccgg
     2581 ccttgcctgc gaggaggatg cgcagtcccg tctggtcctg caccaccgcg gcaagtggaa
     2641 actggagccg gggccgtttg acgaggagcg ctttgccaac ctgccggata aaggctggag
     2701 cctgctggta cagggcgtca accatttcct gccggaagcg gatgaactgc tgcatcgttt
     2761 cgattttatc ccgcgcaccc gcctcgacga ccttatggtc agccttgccc cggacggcga
     2821 cggcgtcggg ccgcacttcg attcctacga cgtgttcctg ctgcaaggcc gcggcaagcg
     2881 gctctggcgc gtcagcagcc agcaggacca tgagctggtg aaagatgcgc cgctgcgtat
     2941 tctgaagaat ttcgagacca atgaagagtg gctgctggag ccgggcgaca tgctctatct
     3001 gccgccgcgg ctggcacact ggggcattgc cgtgggcgaa tgcatgacct ggtccgtcgg
     3061 cttccgcgcg ccgacggcgc aggagctgtc gacccagttt ctggcttacc tgcaggacca
     3121 tctctgcgtc gaaggcatgt acgccgaccc cgatttgcag ccgcagcagc acccggcgca
     3181 aatcggcgag cagatgctgg gcaaggtaca aaacatgctg caagaaatac gttggaacga
     3241 ccatgacgtg acgcgctttc tcggcagcta cctgaccgag cccaagccgc atatcgtttt
     3301 cgatcgtcca ccccgcatgg ggctggacag gtttcgtgag aaactggcgc ggcacgggat
     3361 caggcttgat ctgaaaagcc agatgttgtt tcatggagcc ctcatgttca tcaacggcga
     3421 atcggtcgaa gcgaatagcg ccacgctggc ctgcctgacc acactggccg accatcgccg
     3481 cctgccgcct tcgccgggat tgccggagcc ggcgatcgga ttgctgcatc aatggcatgc
     3541 ggcgggctac ttgggctttt agcataaagg gggagtcatg ccagaacaag caagtgcgga
     3601 gaacaatctc aagcaacacc tcatgctgga aggcgaggtg gaatacgagc gggcgatcga
//
LOCUS       DAOMFA010000151         3637 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_3572_length_3637_cov_5.170017, whole genome shotgun sequence.
ACCESSION   DAOMFA010000151 DAOMFA010000000
VERSION     DAOMFA010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 3637)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3637)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3637
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3572_length_3637_cov_5.170017"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..475
                     /locus_tag="PKW44_07420"
     CDS             <1..475
                     /locus_tag="PKW44_07420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR51449.1"
                     /translation="ASSETTDAQASQTSQASTPHPHPGKSSPERVPNAEKSSDAKESR
                     NPACATCGVIESIDMVEQEGEGSGLGAVAGGVTGGLVGNQIGRGRGNTVMTILGIGGG
                     AYAGHTIEKKMKSTTSYVIKVRMQDGSYRTVTKQSKPDYAVGDHVKIVNDNLAAA"
     gene            742..1404
                     /locus_tag="PKW44_07425"
     CDS             742..1404
                     /locus_tag="PKW44_07425"
                     /inference="COORDINATES: protein motif:HMM:NF016836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HQR51450.1"
                     /translation="MPHPQPHIYRQQTLVRLLAIAVAGGSSNAFAIGTLNDQIQTPLI
                     GEFRKLDRNSDTKLSNDEACRDSDIVHRFSKADGNHDGTLSAEEYGKFKSAAQRARLE
                     TYLDDSGVTAKVKAELLKDIGTKGLSISVETYQGRVILSGFVDTAQQSRRAVEIASGI
                     RGVHSITNSLLVKKTEGQAAQTPSTPNATAPVLRTAPTNAASARRTYISTLAPAPKSK
                     VA"
     gene            1497..1823
                     /locus_tag="PKW44_07430"
     CDS             1497..1823
                     /locus_tag="PKW44_07430"
                     /inference="COORDINATES: protein motif:HMM:NF013179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HQR51451.1"
                     /translation="MNEPTENMAVLMLNDNGMIYGCNKACEKLFGYLSGEIIWKHISM
                     LLPQLAGIALMSGQQINPRLHFLTHIGHQFEVVGLGGIHFASQLFINEVENLGQHYLR
                     LIISPA"
     gene            complement(1971..2765)
                     /locus_tag="PKW44_07435"
     CDS             complement(1971..2765)
                     /locus_tag="PKW44_07435"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HQR51452.1"
                     /translation="MQQYLDLMRHVLEHGHKKEDRTGTGTLSVFGHQMRFDLAAGFPL
                     LTTKKVHLKSIIHELLWFLQGSTNITYLKEHGVTIWDEWADENGNLGPVYGYQWRNWP
                     KPDGGHIDQITQVVEAIRKNPDSRRLIVSAWNVADVDRMKLPPCHAFFQFYVADGKLS
                     CQLYQRSADIFLGVPFNIASYALLTMMVAQVCGLEPGDFVHTLGDAHLYLNHLEQTRE
                     QLSRTPRHLPAMRINPEIRDIFGFRFEDFVLENYDPHPPIKAPVAV"
     gene            complement(2775..>3637)
                     /locus_tag="PKW44_07440"
     CDS             complement(2775..>3637)
                     /locus_tag="PKW44_07440"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168747.1"
                     /note="catalyzes the formation of L-threonine from
                     O-phospho-L-homoserine; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HQR51453.1"
                     /translation="DPNIYNIAVKGVFDDCQDIVKAVSNDHIFKAQHKIGAVNSINWA
                     RVAAQIVYYFKGYFAVTRSNAEKVSFAVPSGNFGNVCAGHIARMMGLPIAKLVVATNE
                     NDVLDEFFRTGIYRPRDAAHTYHTSSPSMDISKASNFERFVFDLVGRSGARMRELWGK
                     VESGGAFDLNDGGYFAKVADYGFVSGASNHALRMKTIRWVNEAYGVTIDTHTADGLKV
                     ALEYREKDVPMVVLETALPAKFEEAIQEALGRAPERPADLAGLEDLPQRFVVMGADVD
                     TVKQFIVEHT"
BASE COUNT          806 a         1080 c         1030 g          721 t
ORIGIN      
        1 ggcatcttca gaaactaccg atgcccaagc ctcgcaaacc agccaagcgt ccacccccca
       61 cccacaccct ggcaagtcga gtcccgaacg agttcccaac gcggaaaaat cgtctgatgc
      121 caaggaaagc agaaatcccg cctgtgccac ctgtggcgtc atcgaatcca tagatatggt
      181 ggagcaggag ggcgaaggca gcggcttggg agctgtcgca ggcggagtca ctggcggtct
      241 ggtcggcaac cagatcggca ggggcagggg caataccgtg atgaccattc ttggcatcgg
      301 tggtggggcc tatgccggcc ataccatcga aaagaaaatg aagtccacga cttcctacgt
      361 catcaaggta cgaatgcagg atggctcata ccgtaccgtc acaaaacaaa gcaaacccga
      421 ttacgcagtt ggcgaccacg tcaagatcgt caacgacaac ctcgcggcgg catgacctta
      481 atctccacgc cccctgcaag ccgcttcccc acgtcaagat ggcttgcgaa gtgattctgt
      541 ctcatgcttg agacagaaaa gttctttcca aatcagtttg ttaaacacgc atcccgcgca
      601 ggcgtcgcct tgcggcgaca tcagcaaggc cgccacacct ccatgaaaca tgtaactcat
      661 tgttatttaa agagtataaa agcatggcat ggcgcttgct cactggtatt gcattaacca
      721 ccgaatggag ttctcgacaa catgccccac ccccaacccc acatctatcg ccagcagacg
      781 ctggtaaggc tgctggcgat tgccgtggca ggcggttcca gcaatgcatt cgccataggc
      841 acgctcaacg accagatcca gacaccgttg atcggcgaat tccgcaagct ggatcgcaat
      901 agcgacacca aactgtctaa tgatgaagcg tgccgcgaca gcgatatcgt gcatcgtttc
      961 agcaaggcgg acggcaatca cgatggcacg ctgtcggctg aggaatacgg caagttcaag
     1021 agcgcagcac agcgcgccag gctggagact tacctggacg acagcggcgt aaccgccaag
     1081 gtcaaggctg aactgctgaa agacatcggc acgaaaggct tgtccatcag tgtggaaacc
     1141 tatcagggcc gcgtcattct gagcgggttc gtggataccg cacagcagtc gcgccgcgcc
     1201 gttgaaatcg cctcaggcat ccgcggggta cacagcatca ccaacagcct gttggtgaag
     1261 aaaaccgaag gtcaggcagc acaaactccc agtacgccga atgccaccgc ccctgtcctg
     1321 cgcacggctc cgacaaacgc tgccagtgct cgccgcacct acatttcaac attggccccg
     1381 gcccctaaga gcaaagttgc atgacatcac cgaccctgcc aacaggccca gtctaaagtc
     1441 tgggaacact gaaatcagcg ccgatcgaac cgacccgcta ttgaaaggag aagcccatga
     1501 acgagcccac cgaaaatatg gccgtgctga tgcttaacga taacggcatg atctacggct
     1561 gtaacaaagc atgcgaaaaa ctgttcggtt acctgtccgg cgaaatcatc tggaagcata
     1621 tttccatgct gctaccacaa ttggcaggca ttgcactgat gagcgggcaa cagatcaatc
     1681 cgcgcctgca ctttctcacg catattggcc accagttcga agtggtcggc ctgggtggca
     1741 tccatttcgc cagccagctg ttcatcaacg aggtggagaa ccttggccag cactacctgc
     1801 gcctcatcat cagtccggct tgagaaaagt acagcctgac taagccacac cccctttgcc
     1861 ggatgacatc aacttgctcg ctccatgatg ccctcccctc agcgagtcgt cttccaaccc
     1921 cgggacggct cgcttttttt cgatgtttcg gcggagtgat taacggccgg tcagactgcg
     1981 accggcgcct tgatcggcgg atgcgggtcg tagttttcca gcacgaaatc ctcgaacctg
     2041 aagccgaaga tatcccttat ctcgggattg atgcgcatgg cgggaaggtg gcgcggcgtg
     2101 cgtgacaact gctcgcgcgt ctgctcgagg tgattgaggt aaaggtgggc gtcgcccagc
     2161 gtatgaacga aatcgcctgg ctcgaggccg cacacctgcg ccaccatcat ggtcagcagg
     2221 gcgtaggagg cgatgttgaa cggcacgccg aggaagatat ccgcgcttct ttgatataac
     2281 tggcaagaga gtttgccgtc agccacataa aactggaaaa aggcatggca gggaggcagc
     2341 ttcatgcggt ccacgtccgc cacgttccag gctgaaacga tcaggcgacg ggaatcggga
     2401 tttttcctga tggcttccac cacctgcgta atctggtcga tatggccgcc gtccggcttc
     2461 ggccagttgc gccactgata gccgtaaacc gggccgaggt tgccgttttc gtccgcccac
     2521 tcgtcccaga tggtaacgcc gtgttccttg agataggtaa tgttggtact gccctgcagg
     2581 aaccacagca gctcgtggat gatggacttg aggtggactt tcttggtggt cagcaagggg
     2641 aagccggcgg ccaggtcgaa gcgcatctgg tggccgaata ctgacagcgt gccggtaccg
     2701 gtgcggtctt cctttttgtg gccgtgctcc aggacatggc gcatgaggtc aagatattgc
     2761 tgcatttgtt ctttctaggt gtgctcgacg atgaactgct tcaccgtatc gacatccgcc
     2821 cccatcacca cgaagcgctg cggcaggtct tccaggccgg ccaaatctgc cggacgttcc
     2881 ggagcacgtc ccagcgcctc ctgaatggcc tcctcgaact tggccggcag cgcggtttcc
     2941 agaaccacca tgggcacgtc cttttcgcgg tattccagcg ccaccttgag gccgtcggcg
     3001 gtatgggtgt cgatggtgac gccgtaggct tcgttcaccc agcggatggt cttcatacgc
     3061 agggcgtggt tgctggcgcc ggagacaaag ccgtaatccg ccaccttggc gaaatagccg
     3121 ccgtcgttga ggtcgaaggc gccgccgctc tccaccttgc cccacagttc gcgcatcctg
     3181 gcgccgcttc tgcccaccag gtcgaacacg aagcgctcga agttggaagc cttgctgatg
     3241 tccatggacg ggctgctggt gtggtaggta tgggcggcat cgcgcgggcg gtaaatgccg
     3301 gtgcggaaga actcgtccag cacgtcgttt tcgttggtcg cgaccaccag tttggcgatc
     3361 ggcagcccca tcatgcgcgc gatgtggccg gcgcagacgt tgccgaaatt gccggacggc
     3421 acggcgaagc tgaccttctc ggcgttgctg cgggtgacag caaagtaacc cttgaagtaa
     3481 tacacgatct gcgctgcgac gcgcgcccag ttgatggaat tgaccgcgcc gatcttgtgc
     3541 tgcgccttga aaatatggtc gttggacacc gccttgacga tatcctggca gtcgtcgaac
     3601 acgcccttga ccgcgatgtt gtagatgttg gggtctt
//
LOCUS       DAOMFA010000152         3623 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_3588_length_3623_cov_5.096413, whole genome shotgun sequence.
ACCESSION   DAOMFA010000152 DAOMFA010000000
VERSION     DAOMFA010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 3623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3623
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3588_length_3623_cov_5.096413"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            32..2518
                     /locus_tag="PKW44_07445"
     CDS             32..2518
                     /locus_tag="PKW44_07445"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HQR51454.1"
                     /translation="MKKTDSKLPPAISSEEVRTGLELPALQRAFEDHQRYTLGRIPAS
                     VTANDRYLAVAYAVRDRLTARWAATLEKALGNGGKVVAYLSAEFLLGPQLACNLFNLG
                     IFDEVADAIRASGNDPQVLFDLEGEPGLGNGGLGRLAACYMESLATLEVPAVGYGIRY
                     EFGIFDQVIEEGRQVEITDKWLRFGNPWEIAKPEKSLEIKLGGHTEGYRDAAGHYRVR
                     WIAGQVVKGVPYDTPILGYRVNTCNTLRLWTAEAVESFDFAAFNTGDYYRAVDDKVSS
                     ENLTKVLYPNDESMQGKRLRLEQQYFFVSCSLQDMLRIHLRLGRKLDAFHQHFAIQLN
                     DTHPSIGVAELMRLLVDEHDMGWEAAWEITRNTFAYTNHTLLPEALETWPVDLFGSVL
                     PRHLEIIYEINRHFLEEVRQRYPGDEARLARMSLIDETRGRRVRMAHLATVGSHKING
                     VAELHSELLKASVLKDFFDLWPKKFDNVTNGVTLRRFVGVSNYRLSQLLKPRIGRGWL
                     KDPKELRALEQLADDPGFQQEWRVVKHFNKERLAALIEARTGHRVDPDSMFDIQVKRI
                     HEYKRQHLNLLHVITLYQRLKNGRDIAPRTVIFGGKAAPGYFQAKLIIRLINGVTRVV
                     NSDPDMRGRLKVVFFPNFNVKNAHFIYPAADLSEQISTAGKEASGTGNMKFTLNGALT
                     IGTLDGANVEIREEVGTDNFFLFGMNAAEVAQCKAQGYHPYNYYQADDELRQAIDLIA
                     DGVFSDGDTDLFRPLVENLLHQDDYLLLADYRAYMDCQGEVDKAWGDQQRWTRMSILN
                     VARAGKFSSDRAIHEYCERIWGAQPLPIGL"
     gene            2608..3360
                     /gene="tpiA"
                     /locus_tag="PKW44_07450"
     CDS             2608..3360
                     /gene="tpiA"
                     /locus_tag="PKW44_07450"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HQR51455.1"
                     /translation="MRRKLVVGNWKMHGDLKSNEELLKTLVAGLRDYNRADYAVCVPH
                     PYLFQAQSVLSGTNIAWGGQNMNQYEAGAFTGSVAPHMLTDFGCTYVIIGHSERRGLC
                     HEDDALVAAKFESAVRAGLKPILCMGETLEDHQAGKAEQVVGRQLDAVLLQVGVKKLK
                     FGVLAYEPVWAIGTRVGAMPDKAQAIHAFIRKRVAQHDSDVASCLRILHGGGIRVANA
                     AQLLAMPDIDGALLGNASLVAEQFIAICRVAN"
     gene            3380..>3623
                     /gene="secG"
                     /locus_tag="PKW44_07455"
     CDS             3380..>3623
                     /gene="secG"
                     /locus_tag="PKW44_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HQR51456.1"
                     /translation="METIVWVVHVIAALGVIGLVLLQHGKGADMGAAFGSGASGSLFG
                     VSGSANLLSRATAAMVTVFFITSVTLAYLASHGSKHA"
BASE COUNT          750 a         1015 c         1084 g          774 t
ORIGIN      
        1 tgttgatatt gaacttcagg gatcctcagc catgaaaaaa accgattcca agttaccccc
       61 tgccataagt tccgaggaag tgcgtaccgg cctggaactg ccggcgctac agcgggcgtt
      121 cgaggatcat cagcgctata ccctaggacg cattccggcc agcgtgacag ccaatgaccg
      181 ctatcttgcc gttgcctatg ctgtgcgcga ccggctgact gcgcgctggg cagccactct
      241 ggaaaaggct ctgggtaacg gtggcaaggt agtggcttat ctttcggcgg aatttctgct
      301 tggcccgcag ttagcctgca acctgttcaa cttggggatt ttcgacgagg tcgccgatgc
      361 tatacgcgcc agcggcaatg atccgcaagt attgttcgat ctggaaggtg agccagggct
      421 cggtaacggc ggtctggggc gactcgcagc atgctatatg gaatcgctgg cgacgctgga
      481 agtccctgct gtcggctatg gcatccgcta tgagttcggt attttcgacc aggtgatcga
      541 ggagggccgc caggtggaaa tcaccgataa atggctgcgc ttcggcaacc cgtgggaaat
      601 cgccaagccg gaaaagtcgc tggagatcaa attgggcggc cacaccgaag gctaccgtga
      661 cgccgccggc cattaccggg tacgctggat tgccgggcag gtggtcaagg gcgtgcctta
      721 cgacacccct atcctgggct atcgggtcaa tacctgcaac accctgcggc tatggacggc
      781 cgaagcggtc gaatccttcg attttgccgc tttcaatact ggcgattact accgtgcggt
      841 ggatgacaag gtcagctcgg aaaacctgac caaagtgctc tacccgaacg acgaatcaat
      901 gcagggcaag cggctgcgtc tggagcagca gtattttttc gtctcctgct ccttgcagga
      961 catgttgcgt atccatctgc gcctgggaag aaagctggat gcctttcatc agcacttcgc
     1021 catccagttg aacgatactc atccttccat cggcgtcgcg gaactgatgc ggctgctggt
     1081 cgatgagcac gacatgggct gggaagcggc ttgggaaatc acgcgcaaca cctttgccta
     1141 caccaatcac acgctgctgc cggaagctct ggagacctgg ccggtggatc tgtttggcag
     1201 cgtcctgcca cgacacttgg aaattatcta cgaaatcaat cgccattttc tggaagaagt
     1261 gcgccaacga taccccggcg acgaagcgcg ccttgctcgc atgtcgctga tcgatgaaac
     1321 ccggggccgc cgcgtgcgca tggcgcatct ggctacggtc ggctcgcaca agatcaacgg
     1381 tgtggccgaa ctgcacagcg aattgctcaa ggccagcgtg ctgaaggact tcttcgatct
     1441 ttggccgaag aaattcgaca atgtcaccaa cggcgtcacg ctgcgccgct tcgttggcgt
     1501 atccaactac cgtttgtcac agttactcaa gccacgcatc ggccggggct ggctgaagga
     1561 tccgaaggag cttcgtgcgc tggagcagtt ggccgacgat cccggctttc agcaggaatg
     1621 gcgcgtggtc aagcatttca acaaggagcg cctagccgcg ttgatcgagg cgcgtaccgg
     1681 ccaccgcgtc gatcccgact ccatgttcga cattcaggtc aaacgtattc atgagtacaa
     1741 gcgccagcat ctcaacctgc tgcatgtgat taccctctac cagcgtctca agaacggccg
     1801 ggacatcgcg ccgcgcacgg tgattttcgg cggcaaggcg gcacccggct acttccaggc
     1861 caaactcatc atccgtctca tcaacggggt gaccagggta gtcaacagcg accccgatat
     1921 gcgcggtcgg ctgaaagtgg tgttttttcc taacttcaac gtcaagaacg cccatttcat
     1981 ctacccggca gccgacctgt ccgagcaaat ttccaccgcc ggcaaggaag cctccggcac
     2041 cggcaacatg aaattcaccc tcaacggcgc gctcaccatc ggcacccttg acggcgctaa
     2101 cgtggaaatc cgcgaggagg tagggactga caatttcttc ctgttcggca tgaatgccgc
     2161 cgaggtcgcg cagtgcaagg cgcagggtta tcatccctac aactattacc aagcggacga
     2221 tgaattgcga caagcgattg atctcattgc tgacggggta ttttcagatg gcgacaccga
     2281 cctgttccgc cccttggtgg aaaacctgct gcaccaggac gattacctgc tacttgccga
     2341 ttatcgtgcc tacatggatt gccagggcga agtggacaag gcttggggtg accagcagcg
     2401 ctggacgcgc atgtccatcc tcaacgttgc gcgcgccggt aagttttcct ccgaccgcgc
     2461 cattcacgag tactgcgagc gtatctgggg tgcgcagccg ctgccgattg gcctgtagcc
     2521 aggcttggtg cgctgcttta ctttgaccgg gctggcgttg gcgggtatca tgcgcaccac
     2581 ttttctgggt aacaatgaat gcctctaatg cgtcgcaagc tggtagtagg caactggaag
     2641 atgcacggcg acctcaagtc gaatgaggag ttgctgaaaa cgctggtggc gggtttgcgc
     2701 gactacaacc gtgccgacta cgcggtttgc gtgccgcacc cctacctgtt ccaggcccag
     2761 tccgtgctga gcggtaccaa tatcgcctgg ggcggccaga acatgaacca gtatgaggcg
     2821 ggagcgttta ccggttcggt ggcaccgcac atgctgacgg attttggctg tacctacgtc
     2881 atcatcggcc attccgaacg ccgcggactt tgccatgagg acgatgcgct ggtcgccgcc
     2941 aagtttgaat cggcagtgag ggcaggactg aagcccatcc tgtgcatggg agagacgctg
     3001 gaagatcatc aggctggcaa ggctgagcag gtggtagggc gccagcttga tgcggtgcta
     3061 ctgcaagtgg gagtgaagaa gctgaaattt ggcgtattgg cttacgagcc ggtgtgggcc
     3121 atcggcactc gcgttggagc catgcctgac aaggctcagg cgattcatgc cttcatccgc
     3181 aagcgcgtgg cgcagcatga ttcagatgtc gcctcctgcc tcagaattct acatgggggt
     3241 ggcattagag tcgcaaatgc ggcacaatta ctggctatgc cggatattga tggcgcgttg
     3301 ctcggcaatg cttcgttggt agcggaacag tttattgcga tttgccgcgt ggcaaattaa
     3361 gaatttgagt gaaagtttaa tggaaacaat cgtctgggta gtacatgtta tcgccgcgct
     3421 gggcgtcatt ggcctcgtgc tgttgcaaca cggaaagggt gccgacatgg gtgcggcgtt
     3481 tggtagcggc gcttccggga gcctgttcgg agtttccggg tcggctaact tgctgagccg
     3541 cgcgacggcg gccatggtca cggtgttctt catcaccagc gtgacgctgg cttatctcgc
     3601 cagtcacggc agcaagcatg cga
//
LOCUS       DAOMFA010000153         3617 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_3594_length_3617_cov_4.507019, whole genome shotgun sequence.
ACCESSION   DAOMFA010000153 DAOMFA010000000
VERSION     DAOMFA010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 3617)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3617)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3617
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3594_length_3617_cov_4.507019"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1066
                     /locus_tag="PKW44_07460"
     CDS             <1..1066
                     /locus_tag="PKW44_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019899368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQR51457.1"
                     /translation="RYDRFAEWEARWNPQWVTQLGLRSDTVKTDAGKVQGYSPMGGMM
                     GYGNPDNPASIPGAFNAADRKDTDNNIDLTALARFTPSTQETFEAGYARKTRSPNLYE
                     RYTWAYNNTMVMNMNNWYGDGNGYVGNLNLNPEIAHTLSLTGNWHDAAKEDWGFTVAP
                     YYTHIDHYIDAVPCTKVGKTCPARNDGFVNLSLDNQTAHVYGVDVSGKMSLAKTGDYG
                     NFNLAGILNYVRGKNTDTDEDLYNIMPLNLKLAVEQRVGNWTNTIETKLVDAKTHVEA
                     VRKELKTAGYGLLNLYSSYEWKQLRFDVGVENVFDRGYDDPLGGAYVGQGATMGTGVP
                     YGTAVPGMGRSINTALTLKF"
     gene            complement(1132..1590)
                     /locus_tag="PKW44_07465"
     CDS             complement(1132..1590)
                     /locus_tag="PKW44_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013293698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-helix bundle copper-binding protein"
                     /protein_id="HQR51458.1"
                     /translation="MNRRDILKTTGAIALAALAADALATEEHQHDHAAKGGHEGMKNP
                     NQKLISAASDCVLKGELCIDHCLMLLADGEKEMAACARSVHQLLALCSALQQLASYQT
                     PQFKALAKVAMEGCKECEDECRKHEKKHAVCHDCAESCAACYKECKAITA"
     gene            complement(1628..2197)
                     /locus_tag="PKW44_07470"
     CDS             complement(1628..2197)
                     /locus_tag="PKW44_07470"
                     /inference="COORDINATES: protein motif:HMM:NF021517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2244 domain-containing protein"
                     /protein_id="HQR51459.1"
                     /translation="MDQTYLSARQQRLSAQVFSIASITAVLAPPLIMLWIAASIFVYA
                     AIAHHPNPRVVEYLRPAGYRFYGLAGSLVVVLNYTEIMKHWFGGALHMWLAVWLVSLL
                     VIVPMGLRDIVRAAREPWADLTSRGGVRQAMVEIRATTPDAGFSVESRPNCSLSWLGT
                     VYLFAIIALYALVIAMGFALIGAWPVTRA"
     gene            2326..3612
                     /locus_tag="PKW44_07475"
     CDS             2326..3612
                     /locus_tag="PKW44_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQR51460.1"
                     /translation="MEYSGQASPSATLKNLRRLFWLRNVMMAFLSSTALVLLYLNIPL
                     HVLPIVAAVSSMLALNTLTWLRLRRPGNVSEGGLFLQLLGDIATLTALFYFTGGYSNP
                     FVWMYLLPLAIAAVALRTTHVWLIAMIAVGCYSMLVFYHEPLSHLHVHTQEGVGIDIH
                     LAGMWLGFVVSAGVIAFFVERMGRNLREYDQLIAETREKALEGERMLALGTLATAAAH
                     ELGTPLATMAVVAGEMADDCASQPQLAESLSLLRSQIDRCKQILTSITASAGQQRAED
                     GQGQALDDFLRQTLDRWRDTRPAIEAACLLQDRGPTPTIAVDRTLGQALTNLLDNAAD
                     ASPEGIEISCQWDNTRLKLDIRDHGPGLAPEVADKAGTLFFTTKQDQGLGLGLYLARL
                     IFERFGGSVSLETHPPGGTLTRVRLPLDGLLLTGKS"
BASE COUNT          762 a         1141 c         1048 g          666 t
ORIGIN      
        1 ccgctacgac cggtttgccg agtgggaggc acgctggaac ccgcaatggg tgacccaact
       61 tggcctgcgc agcgatacgg taaaaacgga tgccggcaag gtacagggct acagccccat
      121 gggcggcatg atgggctacg gcaacccaga caacccggcc agcatccccg gcgccttcaa
      181 tgccgccgac cgcaaggata ccgacaacaa catcgacctc accgcactgg cacgcttcac
      241 cccgagcacg caggaaacgt tcgaggccgg ctatgccagg aagacgcgtt cgcccaacct
      301 ctacgaacgc tacacctggg cctacaacaa caccatggtc atgaacatga acaactggta
      361 cggggacggc aacggctatg tcggcaacct gaatctgaat ccggaaatcg cacataccct
      421 gagcttgacc ggcaactggc atgacgcggc aaaagaagac tgggggttta cggtcgctcc
      481 ctactacacc catatcgatc actacatcga cgccgtaccc tgcaccaagg tcgggaaaac
      541 ctgcccggcg cggaacgacg gcttcgtcaa cctcagcctg gacaatcaga cggcgcacgt
      601 ttacggcgtg gacgtctccg gcaaaatgtc gctggcaaaa accggcgact acggcaactt
      661 caatctggcg ggaatattga actatgtgcg cggcaagaat accgacacgg atgaagacct
      721 ctacaacatc atgccgctga acctgaagct ggccgtcgaa caacgcgtgg gcaactggac
      781 caacaccatc gagaccaaac tggtggacgc caagactcac gtcgaggccg tgcgcaagga
      841 actgaaaacc gccggctacg gcctgctcaa tctctacagc agctacgagt ggaaacagct
      901 acgcttcgat gtcggggtcg agaacgtgtt cgaccggggc tacgacgatc cgctgggggg
      961 cgcctacgtt ggccagggcg ccaccatggg aacaggcgtg ccctacggta ccgcggttcc
     1021 cggcatgggc cgctcgatca acaccgccct aacgttaaag ttctaggcgg aaacaccctg
     1081 ccggaaaaca aaaaaccgcc ggaaaaccgg cggttttttg ttcggcccgg tctaggccgt
     1141 aatggccttg cactccttgt agcatgcagc gcacgactcg gcgcagtcgt ggcagacggc
     1201 atgcttcttt tcgtgcttgc ggcactcgtc ttcgcattcc ttgcaaccct ccatcgccac
     1261 cttggccagg gccttgaact ggggcgtctg atagctcgcc aattgctgca acgcactgca
     1321 cagggccagc aactggtgca cgctgcgggc gcacgccgcc atttcctttt ccccgtccgc
     1381 cagcagcatc aggcagtggt cgatgcacaa ttcgcctttt agaacgcaat cgctggcagc
     1441 cgagatcagt ttctggttgg ggttcttcat tccctcatgc ccgcccttgg cggcatggtc
     1501 gtgctggtgc tcttccgtgg ccaaggcatc ggcggccagt gctgcgaggg cgatggcacc
     1561 cgtggttttc aggatatccc gtctgttcat gtcgttctcc ttttaaatct tggtctcttg
     1621 tcgaatgtta tgcccgggtg accggccagg cgccgatcag ggcgaagccc atggcgatga
     1681 ccagtgcgta cagcgcgata atggcaaaca ggtagaccgt gcccagccag gacagggagc
     1741 aattgggccg gctttcgacg gagaatccgg catcgggggt tgttgcgcgt atctcgacca
     1801 tggcctgcct cacgccgccg cgagatgtaa ggtcagccca aggttcgcgc gcggcgcgca
     1861 cgatgtcgcg caggcccatg ggcacaatca ccagcaggct gaccagccat accgccagcc
     1921 acatgtggag cgcgccgcca aaccagtgct tcattatctc agtgtaattc agcaccacca
     1981 ccagcgatcc cgccagcccg tagaagcggt agccggccgg gcgcagatat tccaccacgc
     2041 gcgggttggg atggtgggcg atggccgcat aaacgaaaat cgacgcggcg atccacagca
     2101 tgatgagcgg cggcgccagc accgccgtga tggaggcgat gctgaatact tgcgccgaaa
     2161 gcctttgctg cctggcgctc aggtaggtct gatccatggg gttctgccgg tgacagtgat
     2221 ggcagggatt ttaataagga tgacgacatc ctaaaaagtg acatatcgcc gcgcgacaat
     2281 atgccgcagc cccagaggcg gccaaggcgc gctaaaattt gagctatgga atattctgga
     2341 caagcttctc caagtgccac cctcaagaat ttgcgccgcc tgttctggct gcgcaatgtc
     2401 atgatggcct tcctttcctc caccgcgctg gtgctgcttt acctgaatat cccgttgcac
     2461 gtcctgccca tcgttgccgc agtcagcagc atgctggcgc tcaatacgct gacctggctg
     2521 cgtttgcggc gcccaggcaa tgtcagcgaa ggcgggctgt ttttacaact attgggcgac
     2581 attgccacac tgacagccct gttctacttc accggcggct acagcaatcc tttcgtctgg
     2641 atgtatctgt tgccgcttgc catcgcagcg gtcgcgttgc gcaccaccca tgtctggctg
     2701 atcgcaatga tcgcagttgg ctgctattca atgctggtgt tttatcacga gcccctttcc
     2761 catttgcacg tgcataccca ggagggtgtg ggcatcgata tccatctggc tggtatgtgg
     2821 ttagggttcg tggtgagcgc cggtgtgatc gcctttttcg tcgagcgcat gggacgcaac
     2881 ctgcgcgagt acgaccaact gatcgccgag acgcgcgaaa aagcactgga aggcgagcgc
     2941 atgctggcat tgggaacact ggcgacagca gcggcacacg aactcggcac gccgctggcg
     3001 acgatggctg tcgtggccgg ggagatggcg gacgactgcg ccagccagcc gcaactagca
     3061 gaatcgctat cactgctgcg cagccagatc gaccgctgca agcagatact cacctccatt
     3121 accgcctccg ccgggcagca gcgcgccgaa gatggccagg gccaggcatt ggacgatttc
     3181 ctgcgccaaa ccctcgatcg ctggcgggac acccgccctg ccatcgaggc cgcatgcctg
     3241 cttcaggaca gaggcccgac acccaccatc gccgttgacc gcacgctggg gcaggcgctg
     3301 accaacctgc tggacaacgc tgcggacgcc tcgcccgaag gcatcgagat ttcctgccaa
     3361 tgggacaaca ccagactcaa actcgatatc cgcgaccacg gccccggact agcgccggaa
     3421 gtcgcggaca aggccggcac acttttcttc accaccaagc aagaccaggg actgggattg
     3481 ggcctctacc ttgcgcgtct gattttcgaa cgcttcggtg gcagcgtgag cctggaaacc
     3541 cacccgcccg gcggcacgtt gacccgcgtc aggttgccgc tcgatggcct gctactcaca
     3601 ggcaaatcat gagccac
//
LOCUS       DAOMFA010000154         3599 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_3618_length_3599_cov_4.369921, whole genome shotgun sequence.
ACCESSION   DAOMFA010000154 DAOMFA010000000
VERSION     DAOMFA010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 3599)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3599)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3599
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3618_length_3599_cov_4.369921"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..262
                     /locus_tag="PKW44_07480"
     CDS             <1..262
                     /locus_tag="PKW44_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020165002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1841 family protein"
                     /protein_id="HQR51461.1"
                     /translation="YFPEMGETNPFLHMSLHLSILEQLSIDQPPGIAVAYQALLKKHG
                     EKHPAQHDLMDCLAETVWRAQRERTAPDSIAYLACIQNAGLR"
     gene            288..848
                     /locus_tag="PKW44_07485"
     CDS             288..848
                     /locus_tag="PKW44_07485"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HQR51462.1"
                     /translation="MFRYRRFLAVFLFLGLLLAVFELSGLRDHFNLEFLQHRILESRI
                     GGLLLFILMFSLGNLIQIPGWVFLAAAVLALGRTWGGVATYVAASVSCAITFLAIRWI
                     GGDALRQLDNKIAARILGRLDAHPITSVALLRVLFQTVPALNYALAMSGVKFRNYLIG
                     TLLGLPLPIMAYCLFFDYLAKMLKVV"
     gene            complement(921..1070)
                     /locus_tag="PKW44_07490"
     CDS             complement(921..1070)
                     /locus_tag="PKW44_07490"
                     /inference="COORDINATES: protein motif:HMM:NF024600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR51463.1"
                     /translation="MMKMVDANGDHMVSKEEFLKFQETIFIQMDKKGDGMVDAQNWLR
                     KATGN"
     gene            complement(1067..1201)
                     /locus_tag="PKW44_07495"
     CDS             complement(1067..1201)
                     /locus_tag="PKW44_07495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR51464.1"
                     /translation="MNTQFKRMSIVISALLSTVAFSTPASADTMLGTGDYNRELHTMA
                     "
     gene            1423..1998
                     /locus_tag="PKW44_07500"
     CDS             1423..1998
                     /locus_tag="PKW44_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020182881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HQR51465.1"
                     /translation="MWPMPSPENTHDWLAEHGDYLYRYALARLRAPHLAEDAVQETLL
                     AAMQGSGYAGKSSARTWLTGILKHKIIDQFRRQQRETALEEPEEIADDAPGMDEFFEG
                     RHWSEPPQSWGEPASLLQQDQFLQILQDCMDKLPKKLARIFLLREVEETDNDEICKEL
                     EISPTNAWVMLYRARMGLRKCLELNWIGEGR"
     gene            2040..2291
                     /locus_tag="PKW44_07505"
     CDS             2040..2291
                     /locus_tag="PKW44_07505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HQR51466.1"
                     /translation="MERPLSLQLRLALRLHLLLCDACTQFSRQLVLLRAAVGSLSRRI
                     ENDHSLVLPDQARERIAGALAVRAQPAGDSGRQPQGGGD"
     gene            2426..>3599
                     /locus_tag="PKW44_07510"
     CDS             2426..>3599
                     /locus_tag="PKW44_07510"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HQR51467.1"
                     /translation="MTKYVFVTGGVVSSLGKGIAAASLGAILESRGIRVTMLKLDPYI
                     NVDPGTMSPFQHGEVFVTDDGAETDLDLGHYERFISSRMGKRNNFTTGQVYESVIKKE
                     RRGDYLGKTVQVIPHITDEIKSNIKRGAEGADVAIVEVGGTVGDIESLPFLEAIRQMG
                     IEEGRGNACYVHLTLLPWIPTAGELKTKPTQHSVKELREIGIQPDILLCRADRAIPED
                     ERRKIALFTNVAPEAVISAVDADSIYKIPGMLHDQMLDEIVCHKLGILAKAADLSAWK
                     KLTDALENPRHEVNIAFVGKYVDLTESYKSLTEALIHAGMHTHSKVRIHYMDSEEIEK
                     HGCDELANMDAILVPGGFGKRGTEGKIAAIRYAREHKIPYLGICLGMQLAVIEFARD"
BASE COUNT          733 a         1027 c         1037 g          802 t
ORIGIN      
        1 atatttcccc gagatgggcg aaaccaaccc ttttctgcac atgagtctgc acctgtccat
       61 cctcgagcag ctttccatcg accagccgcc gggcattgct gtagcctatc aggccttgct
      121 gaaaaagcat ggggagaaac atcccgcgca gcacgacttg atggactgcc tagccgaaac
      181 cgtgtggcgc gcgcagaggg aaagaaccgc gccggattct attgcctacc tggcgtgcat
      241 ccagaacgcc gggctacgct gaaaaatatc tcatttgaag gaaattgatg ttccgctaca
      301 ggcgatttct tgccgttttc ctgtttctgg ggttgctgct ggccgtcttc gaattgtccg
      361 gcctcaggga tcatttcaac ctcgaatttt tgcagcaccg gatcctggag agccggatcg
      421 gcgggctgtt gctttttatc ctgatgtttt cgctcggcaa cctgatccag attccgggct
      481 gggtgttcct ggcagcggca gtgctggctc tggggcgaac ctgggggggc gtcgccacct
      541 atgttgccgc gagcgtttca tgcgccatta ccttcctggc catccgctgg ataggcggcg
      601 atgccctgcg gcaactcgat aataaaattg ccgccagaat acttggccgg ctcgatgccc
      661 acccgatcac aagtgttgcg ctgttgcgag tgctgttcca gactgtgcct gccctcaact
      721 acgcccttgc catgtcgggc gtcaagttcc gcaactactt gatcggcacg ctgctggggc
      781 ttcccctgcc tatcatggcg tattgcctgt ttttcgatta tctggcgaaa atgctgaagg
      841 tcgtttagcc gggtggggcg cccagcggaa aataaaaagg ccggcaattg ccggcccagt
      901 ccatggagat ggatgcagct ttagttgccg gttgccttgc gcagccagtt ttgcgcgtca
      961 accatgccgt cgcccttctt gtccatctgg atgaagatgg tttcctggaa cttgaggaac
     1021 tcctccttgc tgaccatgtg gtcgccattg gcatccacca tcttcatcat gccatggtat
     1081 gcagttcacg gttgtagtcg ccggtgccca gcatggtatc cgcggatgcg ggtgtggaaa
     1141 atgccaccgt ggacaacagt gcgctaataa cgatagacat tcgcttaaat tgagtgttca
     1201 tctgagtctc ctgattgaat gaattaaatt ccctgtcggg aataccatgc ggttcgcatg
     1261 acgtgtgttg cagccatttg tcaggctggc cgagtatccc ttacacgatg gtgcaataaa
     1321 ttcggaagcc gtattgattt ggtccctatt tcagcgggtc tcacggtgga tgaaatctgc
     1381 cgggcgggcg cttaatggaa aaacttgcca tttcgtgatt gaatgtggcc catgccatcc
     1441 cccgaaaata cccatgattg gctcgccgag catggtgact atctctatcg ttacgccttg
     1501 gcgcgtctgc gcgccccgca tttggccgag gatgcagtgc aggagaccct gctggccgcg
     1561 atgcagggta gcggatatgc cggaaagtcc tcggcgcgta cctggttgac tggcatcctc
     1621 aagcacaaga tcattgacca attccgccgt cagcagcgcg aaacggcgct ggaagagcca
     1681 gaagagattg cggatgatgc gccgggcatg gacgagtttt tcgaaggtcg tcactggagc
     1741 gagccaccgc aatcctgggg cgagcccgcc agcctgttgc agcaggatca gttcctgcaa
     1801 attttgcaag actgcatgga taaactgcct aagaagctgg cgcggatttt cctcctgcgc
     1861 gaggtcgagg aaaccgataa cgacgagatt tgtaaggaac tcgaaatttc accgaccaat
     1921 gcctgggtga tgctgtatcg cgcccgcatg ggtttgagaa agtgtctcga attgaactgg
     1981 atcggggagg gccgttgatg ctggattgca aacatgcaag ccacctgctt tcccggtcct
     2041 tggaaaggcc tctttcgcta cagttgcgcc tggcgctgcg ccttcacctg ctgctatgcg
     2101 atgcctgcac ccagttttcc cggcagcttg tgctattgcg tgcagccgtt ggcagcctct
     2161 cgcgccggat cgaaaacgac cacagcctgg ttttgcctga ccaggcgcgc gaacgcattg
     2221 ccggggcgct ggcggtacgg gcacaacccg ccggcgattc tgggcggcag ccgcaaggtg
     2281 gcggcgactg aggcctaaca ggcctgttaa ttccccccat tccggaattc tccgggtaga
     2341 tcatcgactc actcctattt cattgtgagg ggggatggat tgcggtaaaa tttccccccg
     2401 tccgcctcac ttgaaatcct cattcatgac caaatacgta ttcgttaccg gcggcgtggt
     2461 ttcttccctg ggcaagggta ttgccgctgc gagcctcggc gccattctcg agtcccgcgg
     2521 catccgggtc accatgctca aactggatcc ctatatcaat gtggatcccg gcaccatgag
     2581 cccttttcag catggggaag tgttcgtcac cgacgacggc gccgaaaccg atctcgacct
     2641 gggccactac gaacgcttca tcagttcgcg catgggcaag cgcaacaact ttaccaccgg
     2701 ccaggtgtac gagagcgtca tcaagaagga gcgccgcggc gactacctgg gcaagacggt
     2761 gcaggtgatc ccgcacatca ccgacgagat caagagcaac atcaagcgtg gggcggaagg
     2821 cgccgatgtg gcgattgtgg aagttggagg caccgtcggc gacatcgaat cgctgccgtt
     2881 cctcgaagcc attcgccaga tgggcatcga ggaaggccgg ggcaacgcct gctatgtgca
     2941 cctcaccctg ctgccgtgga ttcccaccgc cggcgaactg aagaccaagc cgacccagca
     3001 ctccgtcaag gaactgcgcg aaatcggtat ccagcccgat attctgttgt gccgcgccga
     3061 ccgcgcgatt cccgaggacg agcggcgcaa gatcgccttg ttcaccaatg tggcgccgga
     3121 agcggtgatt tctgcggtgg atgccgattc gatctacaag atccccggca tgctgcatga
     3181 ccagatgctg gatgagatcg tctgccataa gctgggcatc ttggccaaag ctgctgacct
     3241 gtcggcctgg aaaaagctga ccgacgcgct ggagaatccc aggcacgagg ttaacatcgc
     3301 tttcgtcggc aaatatgtcg acctgaccga atcctacaaa tctctgactg aggcgctgat
     3361 ccacgccggc atgcacactc attccaaggt gcgcattcac tacatggact ccgaagagat
     3421 tgaaaagcat ggctgcgatg agctggcgaa tatggatgcc atcctggtgc cgggtggatt
     3481 cggcaagcgt ggtaccgagg gcaagattgc cgcgattcgc tatgcacgcg aacacaagat
     3541 tccctacctc ggcatttgtc tcggcatgca gctggccgtg atcgagttcg cgcgcgacg
//
LOCUS       DAOMFA010000155         3528 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_3712_length_3528_cov_4.980132, whole genome shotgun sequence.
ACCESSION   DAOMFA010000155 DAOMFA010000000
VERSION     DAOMFA010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 3528)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3528)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3528
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3712_length_3528_cov_4.980132"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..891
                     /locus_tag="PKW44_07515"
     CDS             <1..891
                     /locus_tag="PKW44_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008062521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HQR51468.1"
                     /translation="VTNIGVGDHLGLNYITTVEDLTVVKRVIVQNVAPHGMAVLNAAD
                     PMTAHMADACPGAVTFFAFDRNHPAMATHRAQGRRVVYRDGDAMVAAGEGTEYRVPLS
                     DIPITRNGAIGFQVENAMAATAAAWGLGIDWETIRRGLATFVNDAQTAPGRFNTFDYR
                     GATLIADYGHNPDAIVALVGAIDNLPAKRRMVVISGAGDRRDEDIRRQTELLGDAFDS
                     VILYQDQCQRGRADGEVVALLRAGLQNAKRTTVVEEVNGEFKAIDRSLDLLEPGDLCL
                     VLIDQIDEALAHIAQRVSEA"
     gene            complement(976..1884)
                     /locus_tag="PKW44_07520"
     CDS             complement(976..1884)
                     /locus_tag="PKW44_07520"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HQR51469.1"
                     /translation="MKKTQFIAKAIALVAIMAVPFASLPAQAADTIEIEGVIVSHTGD
                     NVLVRSATGNVTLTVTKATEIEKTKGLLGLRSDSMTADSLIPGLSIKAEAEASGGKTL
                     AKIIKFDGDDLQRASEIQAALAVPQQEAEALKAKVKAQEQVIATQEQEIAANKAANEA
                     QDQQIAASQAEVDKRFSELADYDVKEEITILFDVDNANLSDKAKADIQAAAAKAKTYK
                     GYLIQVAGYADSTGSAKLNQNLSDRRAEAVANYLRQSCDVGISRVLAPVAMSTAKPVA
                     ANESAQGRAENRRVVVKIAVNRGIAQ"
     gene            complement(2091..2861)
                     /gene="lgt"
                     /locus_tag="PKW44_07525"
     CDS             complement(2091..2861)
                     /gene="lgt"
                     /locus_tag="PKW44_07525"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HQR51470.1"
                     /translation="MLTHPQFDPVAIHLGPFGIHWYGLMYLTGFLAFLWLGKLRIRQQ
                     PDWGWSERELDDMLFYGALGVILGGRLGYVLFYQPGQFLAHPADILKVWQGGMSFHGG
                     FLGVLAAMWLFARKTGKRWLAIMDFVAPMVPIGLGAGRLGNFINGELWGKPTDLPWGM
                     VFPQVDALPRHPSQLYEFGLEGVLLFALLWWYSSKPRPTGAVSALFLIGYGTSRFLVE
                     FARQPDSFLGPLALGMSMGQWLSLPMVLAGIWMLRRAR"
     gene            complement(2924..>3528)
                     /locus_tag="PKW44_07530"
     CDS             complement(2924..>3528)
                     /locus_tag="PKW44_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019918527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQR51471.1"
                     /translation="FGSVVQIINHITLLSIILTGAALIREREHGTIEHLLVMPLGPAE
                     IMLAKIWANGLVVVIAASLSLRLIVQGVLQVPIAGSAPLFTVGVLIYLFSVTSLGIFL
                     ATLARSMPQFALLAMPVFIVMNLLSGSNTPFDSMPLAVQHIMLFSPSTHFVRVAQAIL
                     YRGAGIDVVWKELAITAAIGVVLFSAALLRFRKTVSEIQG"
BASE COUNT          664 a         1042 c         1114 g          708 t
ORIGIN      
        1 gtgaccaata tcggcgtcgg cgatcatctg ggcttgaatt acatcaccac ggtcgaggat
       61 ctcaccgtgg tcaagcgcgt gatcgtgcag aacgtggcgc cgcacggcat ggcggtactc
      121 aacgcggcag atccgatgac cgcacacatg gccgacgcct gcccgggggc ggtcactttc
      181 ttcgccttcg atcgtaatca cccggcgatg gcgacgcacc gcgcccaagg caggcgggtg
      241 gtgtaccgcg acggggacgc catggttgcc gccggcgaag gtacggagta ccgcgttccc
      301 ttgtcggaca ttcccatcac ccgcaacggc gcgattggct tccaggtgga aaacgccatg
      361 gctgcgacag ctgccgcgtg ggggctgggt atcgattggg agacgatccg ccgggggctg
      421 gcgacctttg tcaacgacgc gcagaccgcc ccggggcggt tcaacacttt cgactacagg
      481 ggcgctaccc tgattgcgga ctatgggcac aacccggatg ccatcgtggc actggtaggc
      541 gctatcgaca acctgccggc gaagcgccgc atggtagtta tcagcggtgc cggggaccgg
      601 cgcgacgagg atatccgccg ccagaccgag ctgttgggcg atgccttcga tagcgtcatt
      661 ctgtaccagg accagtgcca gcgcggccgc gcggatggcg aggtggtagc gctgctacgc
      721 gcagggttgc aaaatgcaaa gcgtacgacg gtcgtcgagg aggtgaacgg cgagttcaag
      781 gccatcgacc gtagcctgga cttgctcgaa ccgggggatc tttgcctggt gctgatcgat
      841 cagatcgacg aggcgctggc gcatatcgcc cagcgggtgt ccgaggcgta gtacggggat
      901 ggcgggcggc ttgatgcttg gccgctgttg cttgtgaagg gcagggtggg caacccgttg
      961 cccaccctga tttgattact gcgcaatgcc gcggttgacg gcgatcttca ctacgacgcg
     1021 acgattctct gctctgccct gtgcgctttc gttggcggcg acaggcttgg ccgtgctcat
     1081 ggcgaccgga gccagtacgc gggaaatccc gacatcgcac gattggcgca ggtagttggc
     1141 aacagcctcg gcgcggcggt cgctcaggtt ctgattgagc ttggcgcttc cggtggagtc
     1201 cgcgtagcca gctacctgga tcaggtagcc cttgtaagtc ttcgctttgg ccgctgccgc
     1261 ttgtatatct gccttggcct tgtctgacag gttagcgttg tcgacgtcga acaggatggt
     1321 gatttcctct ttcacgtcgt agtcggcgag ttcgctgaag cgcttgtcca cctcggcttg
     1381 cgatgcggcg atttgttggt cctgggcttc gtttgctgcc ttattggctg cgatctcttg
     1441 ttcctgggtc gcgatcactt gctcctgcgc cttgaccttt gccttcagcg cttcggcttc
     1501 ctgctggggt accgccagag ctgcctggat ttcgctggct ctttgcaggt catcgccatc
     1561 gaacttgatg atcttcgcga gggtcttgcc gccgctggcc tctgcctcgg ccttgatgct
     1621 caggcccggg atcagggagt ccgcggtcat gctgtccgac cgcaggccga gcagaccctt
     1681 ggtcttctcg atctccgtgg ccttggtgac ggtcaaggtc acatttcctg ttgcgctgcg
     1741 taccagcaca ttgtcgccag tgtggctcac gatcacgcct tcgatctcta tggtgtccgc
     1801 cgcctgcgca gggaggcttg caaagggcac tgccatgatg gcaaccaatg cgattgcttt
     1861 ggcgataaat tgcgttttct tcattcttat ttcctttcgg gtggcttgga ggcggtcatt
     1921 gtatagggct gatgtatttt aaacaaagaa cattcccaag atttatatat aaccgatata
     1981 aatagcggtt gctgaatgat atttcactag agcacatgca ttttccggat cagcatttaa
     2041 cgcaccatct tgaatggtaa cgaattcctg ccatggccgc cggtacccat tcatctcgcc
     2101 ctgcgcaaca tccatatccc cgccagcacc atcggcaggc tgagccactg tcccatgctc
     2161 atgcccagcg ccagcgggcc gaggaagctg tccggctggc gggcgaattc caccaggaag
     2221 cgtgaagtac cgtagccgat caggaacagt gcggaaacgg caccggtagg gcgaggcttg
     2281 ctggaatacc accatagcag ggcgaacagc agcacgcctt ccagtccgaa ttcgtacagc
     2341 tgcgaagggt ggcgcggcag ggcgtccacc tgtgggaaca ccatgcccca cggcaggtcg
     2401 gtcggtttgc cccacagttc gccgttgatg aagttgccca gccggccggc acccaggccg
     2461 atcggcacca tgggggcgac gaagtccatg atggccagcc agcgcttgcc ggtcttgcgc
     2521 gcgaacagcc acatcgctgc cagcacgccg aggaagccgc cgtggaagct catgccgcct
     2581 tgccagacct tgaggatgtc ggccgggtgg gcaaggaatt gtcccggctg gtagaacagc
     2641 acgtagccca gccgcccgcc aaggatcacg cccagcgcac cgtagaacag catgtcatcc
     2701 agctcgcgtt cgctccagcc ccagtccggc tgttggcgga tgcgcaactt gcccagccag
     2761 aggaaggcca ggaagccggt caggtacatc agaccatacc agtggatgcc gaacgggccg
     2821 agatggatgg cgacgggatc gaattgcggg tgggtcagca tggcgtggaa tttaccatgg
     2881 gcgcgcacga ttgctaaagc gttttccgtt gcgccggact gcctcagccc tgaatctcgc
     2941 tcacggtttt tctgaaccgc aagagggcgg cgctgaacaa taccaccccg attgccgcag
     3001 taatggcaag ttccttccag accacgtcga tgcccgcgcc ccggtaaaga atggcctgtg
     3061 ccaccctcac gaaatgggtg gagggggaaa acaacatgat gtgctgcacg gcaagcggca
     3121 tgctgtcgaa gggcgtgttg ctgccggaga gcaggttcat gacgatgaat accggcatag
     3181 ccagcagcgc gaactgcggc atggatcgtg ccagggtagc gagaaatatt ccgagcgaag
     3241 tgactgaaaa caggtaaatc agcacgccta cggtaaacag aggagcagag cctgcaatcg
     3301 gcacctgcag cacgccctgc acgatcaggc gcagcgagag gcttgcagcg atgacgacga
     3361 ccagcccgtt ggcccagatc ttggccagca tgatttcggc cggccccagc ggcatcacca
     3421 gcagatgctc gatggtgccg tgctcgcgct cgcggatcag cgccgcgccg gtgaggatga
     3481 tggacagcag tgtgatgtgg ttgatgatct gcaccaccga gccgaacc
//
LOCUS       DAOMFA010000156         3521 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_3725_length_3521_cov_4.993364, whole genome shotgun sequence.
ACCESSION   DAOMFA010000156 DAOMFA010000000
VERSION     DAOMFA010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 3521)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3521)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3521
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3725_length_3521_cov_4.993364"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..403
                     /locus_tag="PKW44_07535"
     CDS             <1..403
                     /locus_tag="PKW44_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HQR51472.1"
                     /translation="ALLRAEALGLDSGQYQICFQSRFGRAEWLKPYLAPTLVQLGKQK
                     TGRVDVVCPGFSSDCLETLEEIAMEGKATFLNAGGGEYHYIPALNEREDWIHTLADIA
                     EQHLQGWLEQEPDAGELQQSRLRALAMGAKH"
     gene            446..1414
                     /gene="rfaD"
                     /locus_tag="PKW44_07540"
     CDS             446..1414
                     /gene="rfaD"
                     /locus_tag="PKW44_07540"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HQR51473.1"
                     /translation="MIVITGGAGMIGSMIAWQLNQQGRDDLVIADRQKHAEQWQNLCH
                     RRYAEYLDKDELAAWLESGVKVDAIIHMGAISATTERDWNRLLEDNIRYSQSLWDWCS
                     RNGVPFLYASSAATYGGGEQGYLDAGIDNLRPLNAYGYSKQFFDQWALRQAEAGNAPP
                     LWAGFKFFNVYGPNEYHKERMASVAFHSFNQFRDTGTVKLFKSDRPEVEDGMQRRDFV
                     YVKDAAAVVCWFLHGTAHSGIYNLGTGQARAFKDLATAVMVSSGREPHITWIAMPDDL
                     KGKYQYYTQADMDKLRAAGYDRPFHTLEEGVRDYVQNYLMQPDPYC"
     gene            1425..1988
                     /locus_tag="PKW44_07545"
     CDS             1425..1988
                     /locus_tag="PKW44_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HQR51474.1"
                     /translation="MNRSDIITSALIAHQTVIAQLEPLLPAIYALGERLRNCLNNGNK
                     ILLMGNGGSAADCQHIAAEIVGRYKRERRGLPAIALTTDTSILTSVGNDYGFQHIFSR
                     QIEALCARDDVVIGLSTSGNSANVVAGIEAAKQAGAYTVGLTGAGGGRLADLCDVTLA
                     MPSSDTPRIQEAHILIGHILCELAEAE"
     gene            2005..3471
                     /gene="hldE"
                     /locus_tag="PKW44_07550"
     CDS             2005..3471
                     /gene="hldE"
                     /locus_tag="PKW44_07550"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HQR51475.1"
                     /translation="MQQRLIEAVKHGFPDDKNWALVIGDLMLDRYLWGEVERISPEAP
                     VPVVLLKRETECAGGAANVAANLAGLGVKTRLVGFIGADDDGSRLKRAIHDLKIDTQG
                     LVHAETHATIAKTRVIGGHQQMMRLDRESRAAYSQKDAEALLGGIYRQLADHPSVVVL
                     SDYAKGVLTPELCQALITEAKRLGIPVLVDPKGHDYSKYSGATALTPNKKEAAEACQV
                     DVRNNAALLAAAAQMRSDLKLDFLAVTRGEEGVSLIEDGHALHIPATAKQVFDVSGAG
                     DTVIATLAAGLVAGLQRREALHLANLAAGVVVGKIGTVPIKREELLHELTLEDTSEQA
                     DKVCGLPQLLAKVARWHNAGERIVFTNGCFDLLHAGHVTYLEQARKLGDHLVLGLNTD
                     RSVSALKGPTRPVIHEQDRARVLAALEAVDAVILFDEDTPLELIRQIKPDVLAKGSDY
                     SEDQVVGGAEVRSWGGKVALVDIVPGRSSSIIIEKLAV"
BASE COUNT          737 a         1137 c         1074 g          573 t
ORIGIN      
        1 cgccctcctg cgggcggaag ccttgggact cgactccggc cagtatcaaa tctgcttcca
       61 gtcgcgattc ggccgcgccg aatggttgaa gccctatctg gcaccgacgc tggtgcaact
      121 gggaaaacag aaaaccgggc gggtggacgt ggtctgcccg ggtttttcca gcgactgcct
      181 ggaaaccttg gaagaaatcg ccatggaagg caaggccacg tttctcaatg ccggcggcgg
      241 ggaataccac tacatcccgg cgctcaacga gcgcgaggac tggatccaca cactggcaga
      301 catcgccgag caacacctgc agggctggct ggaacaagag ccggatgccg gcgaactgca
      361 gcaatcccgg ctgcgcgcgc tggcgatggg cgccaaacac tagcgctttg ttatactagc
      421 gtttttatat cggcatattc ccaacatgat cgtcattacc ggtggcgccg gcatgatcgg
      481 cagcatgatc gcctggcaac tcaatcagca gggccgcgac gacctcgtca tcgccgaccg
      541 gcagaagcat gccgagcagt ggcagaacct gtgccatcgc cgctatgccg aatacctgga
      601 caaggacgaa ctggcggctt ggctggagtc cggggtcaag gtggacgcca tcatccacat
      661 gggcgccatc agcgccacca ccgagcgcga ctggaaccgc ctgctggagg acaacatccg
      721 ctatagccag tccttgtggg actggtgctc gcggaacggc gtgccgttcc tctacgccag
      781 ttcggccgcc acttacggcg ggggcgagca gggctacctt gacgccggca tcgacaacct
      841 gcgcccgctc aacgcctacg gctattccaa gcagttcttc gaccagtggg cattgcgcca
      901 ggccgaggcc ggcaacgcgc cgccgctgtg ggcgggcttc aagtttttca acgtctacgg
      961 ccccaacgag taccacaagg aacgcatggc cagcgtcgcc ttccacagct tcaaccagtt
     1021 ccgggatacc ggcacggtca agctgttcaa gagcgaccgc cccgaggtgg aagacggcat
     1081 gcagcgccgc gatttcgtct acgtcaagga cgccgctgcc gtggtgtgct ggttcctgca
     1141 tggcacagcc cacagcggca tctacaacct cggcaccggt caggcgcgcg cgttcaagga
     1201 cctcgccacc gccgtgatgg tgagtagcgg ccgcgaaccg cacatcacct ggatcgccat
     1261 gccggacgac ctcaagggca agtaccagta ctacacccag gctgacatgg acaaactgcg
     1321 cgccgccggc tacgaccggc cgttccacac gctggaagaa ggcgtgcgcg actacgtgca
     1381 gaactacctg atgcaacctg acccctattg ttaaggaagc cgtcatgaac cgctccgata
     1441 tcatcaccag cgccctgatt gcccaccaga ccgtcatcgc ccaactggaa ccactgctcc
     1501 ccgccatcta cgccttgggc gagcgcctgc gcaactgtct gaacaatggc aacaagatcc
     1561 tgctgatggg caacggcggc agcgccgccg actgccagca tatcgccgcc gaaatcgtcg
     1621 gccgctacaa gcgcgagcgg cgcggcctgc cggccatcgc actgaccacc gacacctcga
     1681 ttctgacttc ggtgggcaac gactacggtt tccaacacat tttctcgcgc cagattgaag
     1741 cgctgtgcgc acgggacgat gtcgtgattg gcctgtccac ctccggcaac agcgccaacg
     1801 tggtagccgg catcgaggcc gccaaacagg ccggggctta tacggtgggg ctgaccggtg
     1861 ctggcggcgg caggctggcc gacctctgtg acgtcaccct ggcgatgcct tccagcgata
     1921 ccccgcgtat ccaggaagcg cacatcctga tcggccacat cctgtgcgaa ctggcggaag
     1981 cagagtagga acaggacgaa cctgatgcaa cagcgattga ttgaagccgt caaacacggc
     2041 tttcccgacg acaaaaactg ggcgctggtg atcggcgacc tgatgctgga tcgctacctg
     2101 tggggcgagg tggagcgtat ttccccggaa gcaccggtgc cagtggtatt gctcaagcgc
     2161 gaaaccgaat gcgccggcgg cgcagccaac gtcgctgcca acctggccgg cttgggcgtg
     2221 aagacacggc tggtcggatt catcggtgcg gacgatgacg gcagccgcct caagcgtgcc
     2281 atccacgacc tgaaaatcga cacccagggt ctggtacatg ctgaaaccca cgccaccatc
     2341 gccaagaccc gcgtcatcgg tggccaccag cagatgatgc ggctggatcg tgaaagccgc
     2401 gccgcgtatt cccaaaaaga tgcggaggca ttgctgggtg gtatatatcg gcaactggcc
     2461 gatcaccctt ccgtagtagt gctgtccgac tacgccaagg gcgtgctgac accggagtta
     2521 tgccaggcgc tgattacgga agcgaagcgc ctgggcatcc cggtgctggt cgaccccaag
     2581 ggacacgact atagcaaata cagcggcgcc actgcgctca cccccaacaa gaaagaagcc
     2641 gccgaggcct gccaggtcga cgtccgtaac aacgccgcgc tgctggccgc cgcagcgcag
     2701 atgcgcagtg acctgaaact ggatttcctc gccgtcaccc gcggcgaaga aggcgtatcg
     2761 ctgatcgagg atggtcatgc cctgcacatt ccggccaccg ccaaacaggt gttcgacgtg
     2821 tccggcgccg gtgacaccgt gatcgccacc ctggctgccg gactggtcgc aggtttgcaa
     2881 cggcgcgagg cgctgcacct cgccaacctc gccgcggggg tggtggtcgg caaaatcggc
     2941 acggtgccga tcaagcgcga ggaactgctg cacgaactga ccctggaaga cacctcggaa
     3001 caggccgaca aggtgtgcgg gctgccgcag ctgctggcca aggtcgcccg atggcacaat
     3061 gccggcgagc gcatcgtatt caccaacggc tgcttcgacc tgctgcatgc cggccacgtg
     3121 acttacctgg aacaagcgcg caagctcggc gaccacctgg tgttggggct caataccgac
     3181 cgctcggtaa gcgctctcaa gggtcccacc cgccccgtca tccacgaaca ggatcgggcg
     3241 cgcgtattgg ctgcactgga agcggtcgac gccgtcatcc tgttcgatga agacactccg
     3301 ctggagctga tccggcaaat caagccggat gtgctggcca aaggcagcga ctacagtgaa
     3361 gaccaagtgg tgggcggcgc ggaggtcagg tcctggggcg gcaaggtagc gctagtggac
     3421 atcgttccgg ggcgcagcag cagcatcatc atcgaaaaac tggcggtttg aatcaggata
     3481 taattcggcg ccatgcatga acatacccca cgccataacg c
//
LOCUS       DAOMFA010000157         3473 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_3795_length_3473_cov_4.746050, whole genome shotgun sequence.
ACCESSION   DAOMFA010000157 DAOMFA010000000
VERSION     DAOMFA010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 3473)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3473)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3473
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3795_length_3473_cov_4.746050"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(278..2290)
                     /locus_tag="PKW44_07555"
     CDS             complement(278..2290)
                     /locus_tag="PKW44_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1926 domain-containing protein"
                     /protein_id="HQR51476.1"
                     /translation="MTKSVALLLGVHAHQPVGNFTHVLDDAHTRCYGPFLQVLHRYPD
                     FRFAIHISGWLLDYMLKHYPQDMQLLKEMVKRGQAELFGAGYTEPVLASIPSRDRVGQ
                     ITKLSGYLHDKLGQKPQGAWLTERVWESTVVPALGDSGIDYVTVDDYHFLCTGKQPHE
                     LNGFYTTEEDGRRLDLFPISEPLRYRLPFSPAQEVVSYLESLADSSGQAAAVYFDDIE
                     KFGIWPETYEWVYEKGWLKDFIEGVLNSSVIHPMKYSDYHAQAKTRGVVYLPTASYSE
                     MNEWTLPVPAAHHYGDMVDREKCEGRWETSKPFVRGGIWRNFFMRFPESNWMHKRMLA
                     LSARYHALPEQAKTPAMLHELYESQANDAYWHGLFGGLYLPHLRRAIYNAIVRLEAML
                     DAVEPRPARSQTDLDLDGNDELFLQNGKLQAVIKLDGSASICELDAYQLAHNFGDSLA
                     RQIEHYHRKVHAESDHSHSGEGIANPHERVSFKHEILACDLATDAHARTLFRDTWSDR
                     EGSPAVAVEYGLAGGGKAASAVFEGHAGNGAISKKLAVKDDSLQVSYRFDKMRSGLFR
                     VEINLAMPSCDGPAGRFRIGDDIPGGFGQPLHLDAMKKIVLEDEVLGGNLRLSSSIPC
                     QFDTYPHFSVSQSEAGFEKIMQAVTLVLTWRMGALAKGVDVELRVE"
     gene            complement(2373..>3473)
                     /locus_tag="PKW44_07560"
     CDS             complement(2373..>3473)
                     /locus_tag="PKW44_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 57 protein"
                     /protein_id="HQR51477.1"
                     /translation="HFHPILPLLLDFNAARDSLHDVALPESPRYPGGVNRAKAHITSA
                     IDSHYRRFGEPPRGMWPAEGGVSDPALLLLAEHGMTWAATGEGVLGNSLHQVYAGALP
                     ERHHYLYKPYLVTDGHQEIVTFFRDDRLSDKIGFEYSKWHGADAVNDFVRELEHIWHV
                     TDPEETPVVSVILDGENAWEYYPYNAYHFLSDLYQALAGHHHIEMSTFSEVVRHCSSV
                     SRNEELLETASFHGANVALENLPTICAGSWVYGSFSTWIGSSDKNRGWDLLCEAKKNY
                     DLAMSSRRLNAAENALAERQMGDCQGSDWFWWFGDYNSAMSVASFDQLYRRNLANLYR
                     LLKLPVPKTLGHVISTGGGDPEGGGTMRRGQD"
BASE COUNT          762 a         1025 c          955 g          731 t
ORIGIN      
        1 atataaagcg cctcacccac taatacacga tcaatcactg ccatttgatt ctcctaaata
       61 aacaataaaa actagatact ctcaagcacg attcgcacca ataaccaccg gcaactcgct
      121 aacgcgtatc tgtctggatg aaaaattcat tgcgccgatc acgtcgcaga ccacggataa
      181 tacacacgcg gcgccacccc ttcggaaaag cgccgcaatt gtaccataat ccacaatgtg
      241 ttatcgggct tctctaaacc agcttcgggt tagcgcccta ttccacccga agttcgacat
      301 ccacaccttt ggccaaagcg cccatacgcc aggtcagcac caatgttact gcctgcatga
      361 tcttctcgaa gccggcctcg gattgcgaaa cactgaaatg cgggtaggtg tcgaattggc
      421 aagggatact gctcgacaat ctgaggttgc ctcccaatac ttcatcttcc agaacaattt
      481 tcttcatggc atcgagatgc agcggttggc caaaaccacc gggaatatca tcgccgattc
      541 tgaagcgtcc tgcggggccg tcgcaacttg gcatggccag attgatttcg acacggaaaa
      601 gaccactgcg catcttgtca aagcgataag agacttgcag gctgtcgtcc ttgacagcta
      661 gcttcttgct gatagcgcca ttgccagcat gcccttcaaa gactgctgaa gccgccttgc
      721 cgccacctgc caagccgtat tcgactgcaa cagcgggaga accttcacga tccgaccagg
      781 tatcgcggaa cagcgtcctg gcatgcgcat ccgtggccag atcgcaggcc agaatttcgt
      841 gcttgaagct gacccgttca tgcgggttgg cgatgccttc gccactgtga ctgtggtcac
      901 tctccgcatg caccttgcgg tggtaatgct cgatctggcg cgccaggcta tcgccaaaat
      961 tgtgcgccag ttggtaggcg tcaagctcgc agattgatgc cgagccgtct agcttgatca
     1021 ccgcctgcag cttgccgttt tgcagaaaca gttcgtcatt gccgtccaga tcgaggtcag
     1081 tctggctgcg tgccgggcgc ggttccaccg catccagcat ggcttccaga cgcacgatcg
     1141 cattgtagat ggcgcgtcgc aaatgtggca gatagagccc gccgaacagg ccgtgccagt
     1201 aggcgtcatt ggcttgcgat tcgtataatt cgtgcagcat ggccggtgtt ttggcttgct
     1261 ccggcaacgc gtgatagcgc gcggacaggg ccagcatgcg cttgtgcatc cagttcgatt
     1321 ccgggaagcg catgaagaaa ttgcgccaga taccgccgcg cacgaacggt ttgctggttt
     1381 cccagcgacc ttcgcatttt tcgcggtcga ccatgtcacc ataatggtgc gctgccggca
     1441 ccggtagcgt ccattcgttc atctcgctat aggaagcagt gggcaggtag acgacgccgc
     1501 gcgtcttggc ctgtgcatgg tagtcgctgt atttcatcgg gtgaatgact gaagaattga
     1561 gcacgccctc gatgaagtcc ttgagccagc ccttttcgta cacccattcg taggtttccg
     1621 gccagatgcc aaatttctcg atatcgtcga aatacactgc cgctgcctgg ccgctgctgt
     1681 cggccagact ctccagataa gagacaacct cctgcgccgg cgaaaacggc aagcgatagc
     1741 gtagcggttc ggaaatcgga aacaaatcca gcctgcggcc atcttcttcg gtagtataga
     1801 agccgttcag ttcatgcggc tgcttgccgg tgcacaggaa gtggtagtcg tccactgtca
     1861 catagtcgat accggaatca cccagcgccg gcaccacggt agattcccaa acccgttccg
     1921 tcagccacgc accctgcggc ttctgaccga gcttgtcatg caggtagccg gacagtttgg
     1981 tgatttggcc gacacggtca cgcgatggga tcgaagccag caccggctcg gtatagccgg
     2041 caccgaacaa ttcggcttgg ccgcgcttga ccatctcctt cagcaactgc atgtcctgcg
     2101 ggtaatgctt gagcatgtag tccagcagcc agccgctgat gtgaatggcg aagcggaaat
     2161 cgggatagcg atgcagcact tgcaggaacg gcccgtagca acgggtatgg gcatcgtcca
     2221 gcacatgggt gaaattgccg accggctggt gggcatgcac ccccagcagt aacgcgacag
     2281 atttggtcaa ttgagggttt ccttagcgta ccactttgaa atgggggcga attttacagc
     2341 aaaaacagca gcttggctat gctgatgaaa catcagtcct gaccacggcg catggtgccg
     2401 ccaccctcgg ggtcgccgcc accggtgctg atgacatgcc caagcgtttt gggaactggt
     2461 aacttgagca aacggtacag atttgccagg ttgcggcgat acagctgatc gaaactggcc
     2521 acactcatgg ccgagttgta atcaccgaac caccagaacc agtccgaacc ctggcaatcg
     2581 cccatctggc gctcggccag cgcgttctct gcggcattaa gccgcctgct ggacatggcc
     2641 aggtcgtagt tctttttcgc ctcgcacaac aggtcccagc cgcgattctt gtccgacgag
     2701 ccgatccagg tgctgaaact gccgtacacc cagctcccag cgcagatggt gggcaggttc
     2761 tccagagcga cattggcgcc atgaaagctt gccgtctcca gcagctcttc atttctggag
     2821 acactggaac aatgacgcac cacctcactg aacgtgctca tctcgatatg atgatgtcct
     2881 gccagcgcct ggtacagatc agacagaaaa tgataggcgt tgtaggggta gtattcccaa
     2941 gcgttctcgc cgtccaggat gacgctgacc accggcgttt cctccgggtc agtcacatgc
     3001 cagatatgtt ccagctcgcg cacgaagtcg ttgacggcat cggcgccatg ccacttggaa
     3061 tactcgaagc cgatcttgtc ggagagccgg tcatcgcgga agaaagtgac aatctcctgg
     3121 tgaccgtcag tcaccagata gggtttgtac aggtaatgat gacgctccgg caacgcgccg
     3181 gcgtagactt ggtgaagact gttgcccagc acgccctcgc cagtggcagc ccaggtcatt
     3241 ccatgctccg ccagcagcag cagcgccggg tccgacacgc cgccctcggc cggccacatg
     3301 ccccgcggcg gctcgccaaa acgccggtaa tggctgtcga tggcggaagt aatatgcgcc
     3361 ttggcacggt tgactccccc cgggtagcgt gggctttctg gcaaggcgac atcatgcagg
     3421 ctgtcgcggg cggcgttgaa atcgagcagc aacggcagga tgggatggaa atg
//
LOCUS       DAOMFA010000158         3461 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_3825_length_3461_cov_4.530534, whole genome shotgun sequence.
ACCESSION   DAOMFA010000158 DAOMFA010000000
VERSION     DAOMFA010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 3461)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3461)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3461
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3825_length_3461_cov_4.530534"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            82..2826
                     /gene="rbbA"
                     /locus_tag="PKW44_07565"
     CDS             82..2826
                     /gene="rbbA"
                     /locus_tag="PKW44_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008060119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HQR51478.1"
                     /translation="MTAAHGPVARLSGVTHRYGKVPALDGVDLEIPAGCVVGFIGPDG
                     VGKSTLLALVSGAREIQTGRVEVLGGSMDSLRHRDAVCPRIAYMPQGLGKNLYATLSV
                     FENVDFFGRLFGQPEQERAWRIQKLLTSTGLAPFADRPAGQLSGGMKQKLGLCCALIH
                     DPELLILDEPTTGVDPLSRRQFWELIDHIRARRPGMSVLVATAYMEEAERFDWLVAMD
                     GGRVLDTGTPVQLKANQASATLEQAFIALLPMEKRRQHQTLAIPPRTAHDGAPAIEAQ
                     RLTRRFGDFVAVDHVSFRIERGEIFGFLGSNGCGKTTTMKMLTGLLPASEGDASLFGR
                     PVDPHELETRKRVGYMSQSFSLYTELTVWQNLDMHARLFHLPADKIPGRIAEMVSRFG
                     LGEYLEARTESLPLGIRQRLSLAVAVIHGSEVLILDEPTSGVDPVARDDFWRLLVDLS
                     RNEGVTIFISTHFMNEAQRCDRISLMHAGTVLAQGAPDELIEAHKAATLEEAFIAYLE
                     DASGTEPTPAVPTTPADTPVQAAPAVREKLSLFSPRRCWAYARREAMEIWRDPIRLAF
                     SLLGPVILMITFGYGISFDVENQAYAALDRDQSAESRAYLENYTSLRYFREMSPLHNY
                     ADLETRMRGGKLRAAIEIPPDFGKNVTRGRPVEVGVWIDGAAPFTAETVRGYIEGVHQ
                     SYLQRLVQRRTGEMPAPLPVNIETRFRYNQDFKSVYAMVPGMLMMLLALIPSMMTAVG
                     VVREKEMGSITNLYVTPVTKMEFLLGKQLPYVAIAFLSYLSLLLLALLLFQIPLKGNF
                     FVLTAGALVYVVGTTGFGLLISSFVKTQVAALFGTAVLIMLPVVQFSGLFTPVSSLSG
                     SAKIMGLLFPSTYFERISIGMFTKGLEFGDIAVNFVALGAIILVYLVLSLSLLRTQER
                     "
     gene            2829..>3461
                     /locus_tag="PKW44_07570"
     CDS             2829..>3461
                     /locus_tag="PKW44_07570"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQR51479.1"
                     /translation="MLTHLQNIYRLGLKELISLRYDVVMVVLIVYFFSYAVIAPAKST
                     QMDLKNASVAVVDEDGSTLSRRIHDALLPPYFLPPKQISAQQIDEAMDSARYTFVIDI
                     PPDFQADLERGRRPTVQVNIDATAMIQAGSGARHIQNIIAQEIAAFTHSTDSQQIKLV
                     VRSLFNPNRESAWFVSVMQVINNITLLAIFLTGAALIREREHGTIEHLLVM"
BASE COUNT          643 a         1084 c         1026 g          708 t
ORIGIN      
        1 acccggcctg cccggctcgg cctactttca actggatgca tccgccgaat ggccagccca
       61 cctgcaggtg aagctgccgc aatgaccgct gcgcacggcc ccgtggcgcg gctgtcgggg
      121 gtaacgcatc gctacgggaa ggtgccggcc ctcgatggcg ttgatctcga gattcctgcc
      181 ggctgcgtgg tcggcttcat cggtcctgac ggcgttggca aatccaccct gctggctctg
      241 gtgtccggcg cacgcgaaat ccagaccggc agagtagaag tgctgggcgg cagtatggac
      301 agcctgcgcc accgcgatgc ggtgtgcccg cgcatcgcct acatgccgca ggggctgggc
      361 aagaacctct atgccacact ctcggtgttc gagaacgtcg atttcttcgg ccgtttgttc
      421 ggccagccgg agcaggaacg tgcctggcgt atccagaaat tgcttaccag caccggcctc
      481 gcgccgttcg ccgaccgtcc cgcgggtcag ctttccggcg gcatgaagca gaagctgggg
      541 ctgtgctgcg cgctgatcca tgatccggaa ctgctgatcc tggacgagcc caccaccggc
      601 gtcgacccgc tgtcgcgccg ccagttctgg gaactgatcg accatatccg cgcccgccgg
      661 ccggggatga gcgtgctggt ggcgacggcc tacatggagg aggcggaacg cttcgactgg
      721 ctggtggcga tggacggtgg cagggttctg gacaccggca ccccggttca gctcaaggcc
      781 aaccaggcat ccgctaccct ggagcaggcc ttcatcgccc tgctgcccat ggaaaagcgc
      841 cggcagcacc agacgctggc catcccaccc agaacagccc acgatggcgc gcctgccatc
      901 gaagcgcagc ggctgacccg ccgtttcggc gattttgtcg cggtggatca cgtcagtttt
      961 cgcatcgagc gcggcgaaat tttcggcttt ctcggctcca acggttgcgg caagaccact
     1021 accatgaaga tgctgacagg cctgctgcct gcatcggagg gcgatgccag cctgttcggg
     1081 cggccggtag atccgcacga actcgaaacc cgcaagcgcg tgggctacat gtcgcaatcg
     1141 ttttcgctct ataccgagct cacggtgtgg cagaacctcg acatgcacgc gcggctgttt
     1201 catctccccg ccgataaaat tcccggcagg attgcggaaa tggtcagccg cttcgggctg
     1261 ggagaatatc tagaggcgcg caccgagagc ctgccgctcg gcatccgcca gcgcctgtcg
     1321 ctggcggtgg cggtgatcca cggctcggaa gtactcatcc tcgacgagcc cacgtccggg
     1381 gtcgatccgg tggcgcgcga tgatttctgg cgcctgctgg tcgatctgtc gcgcaacgaa
     1441 ggcgtgacca tcttcatctc cactcacttc atgaacgaag cccagcgctg cgaccgcatc
     1501 tcgctgatgc acgcgggaac ggtgctggcg caaggcgcgc cggacgagtt gatcgaggcg
     1561 cacaaggccg caacgctgga agaggcgttc atcgcctatc tcgaagacgc ctccggcacg
     1621 gagcccacgc ctgccgtgcc caccacgccg gcggatacgc cagtgcaagc cgccccggct
     1681 gtccgggaaa aactttcgct gttcagcccg cgccgctgct gggcctatgc ccggcgcgag
     1741 gcgatggaaa tctggcgcga cccgatccgc ctcgcttttt cgctgctagg gccggtgatt
     1801 ttgatgatca ccttcggcta cggcatttcc ttcgacgtgg aaaaccaggc ctatgcggcg
     1861 ctggatcgcg accagtcggc ggaaagccgc gcctatctgg aaaactatac cagcctgcgc
     1921 tatttccgcg agatgtcacc gctgcataac tatgccgatc tggaaacgcg catgcgcggc
     1981 ggcaagctac gcgccgccat cgagatcccg cccgatttcg gcaaaaacgt cacgcgcggc
     2041 cgtccggtcg aggtgggggt gtggatcgac ggcgcggcgc cgttcacggc cgaaaccgtg
     2101 cgcggctata tcgaaggcgt gcatcagtcc tacctgcagc ggctggtgca gcgccgcacg
     2161 ggagagatgc cggcacccct gccggtcaat atcgagacgc gcttccgcta caaccaggat
     2221 ttcaaaagcg tgtacgccat ggtccccggc atgctgatga tgttgctggc gctgattccg
     2281 tccatgatga cggcggtagg cgtggtacgc gagaaggaaa tgggctcaat caccaacctt
     2341 tacgttactc cggtgaccaa gatggagttc ctgctgggca aacagctacc ctatgtcgcc
     2401 attgccttcc tgagttatct cagcctgctt ttgctagccc tgctgctgtt ccaaatacca
     2461 ctaaagggta actttttcgt gctgaccgcc ggtgctttgg tctatgtggt cgggactacc
     2521 ggtttcggcc tgttgatatc ctcattcgtc aaaacccagg tcgcggccct attcggcacg
     2581 gcagtgctga ttatgctgcc ggtggtccag ttctcggggt tgttcactcc ggtgtcgtcg
     2641 ctttccggca gtgccaagat aatgggactc ctgttcccca gcacctattt cgagcgtatc
     2701 agcatcggca tgttcaccaa agggctggag ttcggggata ttgccgtcaa tttcgtggcg
     2761 ctaggcgcca tcattctggt ttatctggtg ctgagccttt ctttgctcag aacccaggag
     2821 cgatgaacat gctgacgcat ttgcagaaca tctaccgact tggtctcaag gagctgatca
     2881 gcctgcgcta tgacgtggtg atggtcgtcc tgattgtcta cttcttcagc tatgccgtca
     2941 ttgccccagc caaaagcacc cagatggatc tgaaaaacgc ctcagttgct gtcgtcgatg
     3001 aagacggatc tacactctca cggcgcattc atgacgcgct gctgccgcca tatttcctgc
     3061 cgccaaagca aatcagtgcg cagcagatcg atgaagccat ggacagtgct cgctatacct
     3121 tcgttatcga cattccacct gactttcagg ccgatcttga acggggtaga cgtcccacag
     3181 tacaagtcaa tattgatgcc actgccatga tccaggcagg cagcggtgcg cgccatatcc
     3241 aaaacatcat cgcgcaggaa attgcggctt ttacccactc aaccgattcc cagcaaatca
     3301 aactggtcgt gcgttctctg tttaacccca atcgtgaatc ggcctggttc gtctcggtga
     3361 tgcaggtgat caacaacatc acgctgctgg cgatttttct cacaggtgcg gcgctgatcc
     3421 gcgagcgtga gcacggcacc atcgagcatc tgctggtgat g
//
LOCUS       DAOMFA010000159         3458 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_3831_length_3458_cov_4.117543, whole genome shotgun sequence.
ACCESSION   DAOMFA010000159 DAOMFA010000000
VERSION     DAOMFA010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 3458)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3458)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3458
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3831_length_3458_cov_4.117543"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1175)
                     /locus_tag="PKW44_07575"
     CDS             complement(<1..1175)
                     /locus_tag="PKW44_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HQR51480.1"
                     /translation="MATRQKQDPQLTLVLEARHHNPFAWLGLHREGAGHIFRTFLPYA
                     ARAWLQTGEGWSELKRSQADGLYTWAGQDAPSTPCLLRIEQDGKTFECHDAYTLPPMI
                     SDHDLHLLGEGRLRQAYRLLGAHMAEHQGVAGTRFTVWAPNAERVSVIGDFNRWDGRV
                     HPMRAHGSSGVWEIFIPGITDGALYKFEIRNRHSGHILVKTDPYGTCFELRPGTAARV
                     TSPSSHNWGDGGWLQQRGSADWLHQPMNVYELHLGSWKRTGGNRYLNYRELAEQLVPY
                     VKNLGFTHIELMPISEHPLDESWGYQTTGYFGVTSRFGSPDDFRALVDACHQAGIGVI
                     LDWVPAHFPKDSWALARFDGTALYEHDEQRLGEHQDCGTRIFNYGRNEVRNFLLANA"
     gene            1317..2597
                     /gene="glgC"
                     /locus_tag="PKW44_07580"
     CDS             1317..2597
                     /gene="glgC"
                     /locus_tag="PKW44_07580"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HQR51481.1"
                     /translation="MQQDLSSSRFVSALTKNTVALILAGGRGSRLKDLTNWRAKPAVP
                     FGGKFRIIDFPLSNCMNSGIRRIGVVTQYKAHSLIQHIQRGWGFLRGEFNEFVELLPA
                     QQRVEEEWYKGTADAVFQNLDILRHMGAEYILILAGDHIYKMDYGQMLASHVRNKADM
                     TVACLNVPIEEAKAFGVMGVDEHDRIIQFREKPSNPDSIPGDPTQSLASMGIYVFNAS
                     FLYEQLIRDADDPDSVHDFGQNIIPHLISKYRVFAHRFADSCVNSADGKHYWRDVGTI
                     DAYWEANMELTKVTPELNLYDSSWPIWTYQAQLPPAKFVFDSDDRRGKAVDSLISGGC
                     IISGSCVQRSVLFSDVRVHSYSNIEDSVILPNVEIGRHVILKRVVIDKGSQIPEGMQI
                     GVNPEEDRKRFHVSERGITLVTPEMLGQNLHHAR"
     gene            2716..>3458
                     /locus_tag="PKW44_07585"
     CDS             2716..>3458
                     /locus_tag="PKW44_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HQR51482.1"
                     /translation="MHQPDYRDYLTEEYVLPWTYLHAIKDYTDMAYHLEQNPRAKATF
                     NFVPVLLDQLEDYTEQFAKQQIRDPLLALFLVPDGEPLSAAQRSLIIDSCFKSNHSKM
                     IEPFPNYRCLHELFKKLDAKGGESLDYLSDQYMLDLLVWYHLAWTGESVRREEELIAR
                     LMAKGSQFNESDRRELFDLIGALISGIIPRYKALQEEGRIELSSTPHFHPILPLLLDF
                     NAARDSMHDVALPESPRYPGGVNRAKAHIT"
BASE COUNT          736 a          998 c         1003 g          721 t
ORIGIN      
        1 tgcgcgttgg ccagcaggaa gttgcgcact tcgttgcggc cgtagttgaa aatccgcgtg
       61 ccgcagtcct ggtgctcgcc caggcgctgt tcgtcatgtt catacagggc ggtgccgtcg
      121 aaacgtgcca gcgcccagct atccttgggg aagtgcgccg gcacccagtc cagaatcacg
      181 ccgatgccag cctgatggca ggcatccacc agtgcgcgga aatcgtccgg gctgccgaag
      241 cggctggtga caccgaaata gccggtagtc tgatagcccc aagattcgtc cagcgggtgc
      301 tcgctgatcg gcatcagttc gatatgggtg aagcccagat tcttgacgta gggcaccagt
      361 tgttcggcca gttcgcggta attaaggtag cggttgccac cagtgcgctt ccacgagccg
      421 aggtgtagtt catacacgtt catcggctga tgcagccagt cagcgcttcc acgttgttgc
      481 agccagccgc catcccccca gttgtgactc gatggggaag tcacgcgagc agccgtgccc
      541 gggcgcagtt caaagcaagt gccatagggg tcggtcttga ccaggatgtg gccgctgtgg
      601 cggttgcgga tttcgaattt gtacagcgca ccatcggtga tgcccgggat gaagatttcc
      661 cacacgccgc tggatccatg tgcacgcatc gggtgaacgc ggccatccca gcgattgaaa
      721 tcgccgatga cgctgacgcg ctcggcattg ggtgcccaaa cggtaaagcg agtgccggca
      781 acaccctggt gttcggccat gtgcgcgccg agcaggcggt aggcctgacg cagacggccc
      841 tcgccaagca aatgcaggtc atgatcggaa atcattggcg gcagcgtata ggcgtcatgg
      901 cactcgaagg tcttgccgtc ctgctcgatg cgcagcaggc aaggggtaga gggcgcgtcc
      961 tgaccagccc aggtatacag gccgtccgcc tggctacgct tcagctccga ccagccctcg
     1021 ccagtttgca gccatgcccg cgcagcgtag ggcaggaagg tgcggaagat atgacctgcg
     1081 ccttcgcgat gcaggcccag ccatgcgaat ggattatggt gccgcgcctc aagtactagc
     1141 gtgagctggg gatcttgctt ttgtctggta gccatggtgt tgttaccttt atggtaaggg
     1201 ttctggatta tctctgctta acgtggctat aatggtcaca gggcggtttg gtttgatttg
     1261 gaaacccccg ccttcgatgt gcggataggc caaactataa atcaggagag gcaaaaatgc
     1321 agcaggattt atcatcatcg cgttttgtga gtgcgctgac caagaatacg gtcgcgctga
     1381 ttctggccgg tggcaggggt tcacgcctca aggatctgac caactggcgc gccaagcctg
     1441 cagtcccttt cggcggtaag ttccgcatta tcgactttcc tctctccaat tgcatgaatt
     1501 ccggcatccg ccgcatcggc gtcgtgaccc aatacaaggc gcacagcctg atccagcata
     1561 tccagcgcgg gtggggcttc ctgcgcggcg aattcaacga gttcgtcgaa ttgctgccgg
     1621 cgcagcagcg tgtcgaggag gagtggtaca agggtaccgc cgatgcggtg ttccagaacc
     1681 ttgatatcct gcgccacatg ggcgctgagt acatcctgat tctggcaggc gaccacatct
     1741 acaagatgga ttacggacag atgctggcct cgcacgtgcg caacaaggct gacatgacgg
     1801 tggcctgcct caacgtgccg attgaagaag ccaaagcatt tggcgtgatg ggcgtggacg
     1861 agcacgaccg catcatccag ttccgggaaa aaccgtccaa tcccgattca atccccggcg
     1921 atcccaccca gtctctcgcc agcatgggta tttacgtatt caatgcctct ttcctctacg
     1981 agcaattgat ccgtgacgcc gatgatccgg actcggtgca tgatttcggt cagaacatca
     2041 ttcctcacct gatcagcaag taccgggtat tcgcccatcg cttcgccgac agttgcgtca
     2101 acagcgccga cggcaagcac tattggcgcg acgtggggac catcgatgcc tactgggaag
     2161 ccaacatgga gctgaccaag gtgacgccgg aactcaacct ctacgattcg agctggccga
     2221 tctggactta ccaggcacag ctgccgccgg cgaagtttgt gttcgatagc gatgacaggc
     2281 gcggcaaagc cgtcgactcg ctaatctccg gcggttgcat catcagcggt tcgtgcgtgc
     2341 agcgttcggt actgttctcc gacgtgcgtg tgcatagcta cagcaacatc gaggattccg
     2401 tcatcctgcc caatgtcgaa atcggtcgcc atgttatcct gaagcgggtg gtgatcgaca
     2461 agggttcaca gattccggaa ggcatgcaaa tcggtgtcaa ccctgaggag gatcgcaagc
     2521 gattccacgt ctccgagcgc ggcatcacac tggttacgcc ggaaatgctg ggccagaacc
     2581 tgcaccacgc aaggtaatta gccagtcttt tatagaatgc caggggcggc cggcgcggcc
     2641 gcccttaatc acccacttcg ccgaattcca accatgtcaa aacgacgcaa gctttacctc
     2701 aatctgtatt ggcacatgca tcaacccgat taccgggatt atctcaccga agagtatgtt
     2761 cttccctgga cctatctgca cgccatcaag gattacaccg acatggccta ccatctggag
     2821 cagaacccgc gcgccaaggc aactttcaat tttgtgccgg tgctgctcga ccaactggaa
     2881 gattacacgg agcaatttgc aaaacagcag atacgcgacc cgctgcttgc gctgttcctt
     2941 gtgccggatg gagagccttt gagtgcggcg caacgcagtt tgatcatcga cagctgcttc
     3001 aagagcaatc acagcaagat gatcgagcca tttcccaatt accgctgctt gcatgagctg
     3061 ttcaaaaagc tggatgcgaa gggcggcgaa agtctcgatt acctgtccga ccagtacatg
     3121 ctggacttgc tggtgtggta ccacctggca tggaccggcg aaagcgtgcg gcgcgaagag
     3181 gaactgatcg cccgcctgat ggccaaggga agccagttta acgaaagtga ccgccgtgag
     3241 ttgtttgacc tgatcggtgc gctgatttcc ggcatcatcc cgcgttacaa ggcattgcag
     3301 gaagaagggc ggatagagct ttccagcaca ccgcatttcc atcccatcct gccgttgctg
     3361 ctcgatttca acgccgcccg cgacagcatg catgatgtcg ccttgccaga aagcccacgc
     3421 tacccggggg gcgtcaaccg tgccaaggcg catattac
//
LOCUS       DAOMFA010000160         3431 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_3872_length_3431_cov_4.517476, whole genome shotgun sequence.
ACCESSION   DAOMFA010000160 DAOMFA010000000
VERSION     DAOMFA010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 3431)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3431)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3431
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3872_length_3431_cov_4.517476"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(16..336)
                     /locus_tag="PKW44_07590"
     CDS             complement(16..336)
                     /locus_tag="PKW44_07590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR51483.1"
                     /translation="MTSWQEEKRSAYLYRVLSEQEKDTPRQALFLELAKEADSQAELW
                     AGELAKTGYCMLETYAPDMRVRIVAWLVCRFGPRRMKPILAAINIRGVSVYSSHYSSA
                     RHRS"
     gene            complement(413..1906)
                     /locus_tag="PKW44_07595"
     CDS             complement(413..1906)
                     /locus_tag="PKW44_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019896842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HQR51484.1"
                     /translation="MSLAVLHSRALSGMDAPPVTVEVHLANGLPSFQIVGLPEAEVKE
                     SKDRVRAALLTSHYDFPARRITVNLAPADLPKESGRFDLPIALGILAASGQIPHDKLK
                     DYEFAGELALTGELRPVRGALAMTRKASRDGRAFILPQESAAEAALVKDAVILPALTL
                     LQVCAHLCDREALAAYADGVAATTTHYPDMAEVKGQSLPKRALEIAAAGGHSVLMSGP
                     PGTGKSMLASRFPGILPQMTEEEALESAAIQSLNSGFSRERWKQRPFRAPHHTASGVA
                     LVGGGGNPRPGEVSLAHHGVLFLDELPEFSRSVLEVLREPLESGRITISRAARQADFP
                     ARFQLIAAMNPCPCGYLGHHNNKCRCTPDQVARYRARISGPLLDRIDLHIEVPALRED
                     EMTAAADGEASTHIRTRVEAARQIQTTRQGKPNAQLGSLEVDEYCAPDETGAALLKQA
                     IARLNLSARAYHRILKVARTIADLAGSTAVKSIHIAEAIQYRRGDTY"
     gene            complement(1906..2151)
                     /locus_tag="PKW44_07600"
     CDS             complement(1906..2151)
                     /locus_tag="PKW44_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HQR51485.1"
                     /translation="MLDSKLLDEISGKIRGLLAESPAGDVEKNLRALLQGVFAKLDLV
                     TREEFDVQSQVLQRTREQLSLLEQRLAALEANPPPKE"
     gene            2400..3230
                     /locus_tag="PKW44_07605"
     CDS             2400..3230
                     /locus_tag="PKW44_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TorF family putative porin"
                     /protein_id="HQR51486.1"
                     /translation="MNKKLVTIAVLGALSSLPGLAMAEEAAVAAAAPAPAAEPASPHT
                     FAYNVGLFSQYIFRGLTQTDRKPALQGGVDYTHASGLYAGAWGSNISWLEDSGLYENS
                     SLELDAYGGYRNTFGESGIGYDVGLLYYYYAGDKTAAGSASTGFTNPDTLEAYAALSW
                     KWLQGKVSYVVTNSAWGTDEADGTYYAELNANIPIAESGFTANLHVGRQEFAGSNEGV
                     DNDNYSYTDWKAGVTKSFSNGVNVGGYYTTANTRDSFWTIDNRNLGDDTFTVFVQKVF
                     "
     gene            3306..>3431
                     /locus_tag="PKW44_07610"
     CDS             3306..>3431
                     /locus_tag="PKW44_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HQR51487.1"
                     /translation="MKFVTAIIKPFKLDEVREALSAIGVQGITVTEVKGFGRQKGH"
BASE COUNT          652 a         1030 c         1037 g          712 t
ORIGIN      
        1 tacgcgatac agtcttcaac tccgatggcg ggctgacgag tagtgactgg aatacaccga
       61 caccccacgg atattgatcg ctgccaggat gggcttcatg cggcgcggcc cgaagcggca
      121 caccagccag gccacgattc tgacgcgcat gtccggcgca taggtttcca gcatgcaata
      181 acccgttttt gccagctcac ctgcccacag ctctgcctgg ctgtcagctt ccttggccag
      241 ctccaggaac aacgcctgcc gcggtgtatc tttttcctgt tcggaaagaa cgcggtacag
      301 gtaggcggag cgtttttcct cctgccagct agtcaatacg tgcatggctt attgcccctg
      361 aaaacgcaat aacctgcgga taccactttg cacggaatcc ctcgcttatc cattaatacg
      421 tgtcaccacg ccggtactgg atagcctcgg cgatgtggat ggattttacc gccgtgctgc
      481 cggccaggtc ggcgatggtg cgcgctacct tgaggatacg gtggtaggcg cgtgccgaca
      541 ggttgaggcg ggcaatggcc tgtttcagta gtgcggcacc ggtttcgtcc ggggcgcagt
      601 actcgtccac ttccaagctg cccagttggg cgttgggttt accctggcgt gtagtctgaa
      661 tctgtctcgc tgcttccacg cgcgtgcgga tgtgagtgct ggcttcgccg tctgccgcag
      721 cggtcatttc gtcctcgcgc agggcaggaa cttctatgtg caagtcaatg cgatccagca
      781 gcggaccgga aatgcgcgcg cggtaacgcg ctacctgatc cggcgtgcag cggcatttgt
      841 tgttgtgatg ccccagatag ccgcacgggc aggggttcat ggcggcgatg agctgaaagc
      901 gcgccgggaa atcagcctgg cgcgcggcgc gcgagatggt gatgcggccg gattccagcg
      961 gttcgcgcag cacctccaat accgatctgg aaaactccgg cagttcgtcg agaaacagca
     1021 cgccgtgatg tgccagcgac acctcgcccg gccgcgggtt gccgccgccg cccaccagcg
     1081 ccacgccgga agccgtgtgg tgaggcgcgc ggaatgggcg ctgcttccag cgttcgcgcg
     1141 aaaagccgct gttgagcgac tggatggcgg cggattccag cgcctcctct tcggtcatct
     1201 gcggcagaat gccggggaag cgcgacgcca gcatggattt gccggtaccg ggcgggccgg
     1261 acatcagtac gctgtggccg ccggcagcgg cgatttccag cgcacgcttg ggcagggact
     1321 ggcctttgac ttcggccatg tccgggtaat gcgttgttgt tgcggcaacc ccgtcggcat
     1381 aggctgccag cgcttcgcgg tcgcataggt gagcgcatac ttgcagcagc gtcaaagccg
     1441 ggaggatcac cgcatccttc accagtgccg cctccgccgc gctttcctgc ggcaggatga
     1501 aggcgcgacc gtcgcggctg gccttgcggg tcatcgccag cgcgccgcgc accggccgca
     1561 gttcgccagt cagcgccagt tcgccggcga attcataatc cttgagcttg tcatgcggga
     1621 tttgcccgga agccgccagg ataccgaggg cgatcggtag gtcgaagcgt ccgctttcct
     1681 tcggcaggtc ggccggcgcc aggttgaccg tgatgcgacg tgccggaaaa tcatagtgcg
     1741 acgtcagcag cgcggcgcgc acccgatcct tgctttcctt gacctcggct tccggcagcc
     1801 cgacgatctg aaaactgggc aggccgttgg ccagatgcac ctccaccgtg accggcggcg
     1861 catccatacc ggacagcgcc cggctgtgca gcacggcgag gctcattatt ccttgggcgg
     1921 cgggtttgct tccagcgcag ccaagcgctg ctccagcagg ctcaactgtt cgcgagtgcg
     1981 ctgcagtacc tgactttgta cgtcgaactc ttcacgtgtg accagatcca gtttggcaaa
     2041 gacgccttgc aacagcgcgc gcagattctt ttccacgtcg ccggccggac tttcggccag
     2101 caggcccctg atcttgccgc tgatttcatc gagtagttta ctgtccagca ctttgattct
     2161 ccttattcaa ctctgccttg cagtgtacca aagttaagcc atggtgcgct tttagtgggt
     2221 atgcaccctg cttgtgcata cccctgaaat ggcgcactat tattgggcga gccattgcgg
     2281 cgggtgctgc gaggttagat taactgcatg atattaaaat gtttaatgac atggcacggg
     2341 cattgcttag tgtaaacgcc aaagtttgta tcgcaactat caactcgaaa ggaaatttca
     2401 tgaacaagaa gcttgtcact attgccgtgc tgggtgcgct ttcatcgtta cctggtcttg
     2461 ccatggccga ggaggcagcg gtagctgctg ccgctcccgc gccagctgcg gaaccggcat
     2521 ctccgcacac tttcgcctac aacgtcggcc tgttcagcca gtatatcttc cgcggtctga
     2581 cccagactga caggaagccg gctttgcagg gcggcgtgga ttatacccac gccagcggcc
     2641 tgtatgcggg cgcctggggt tccaacatca gctggctgga agacagcggc ctgtacgaaa
     2701 actccagcct ggaactcgac gcttacggcg gctaccgcaa cacgtttggc gagagtggca
     2761 tcggctacga cgtcggcctg ctgtactact actatgccgg cgacaagact gctgctggct
     2821 cggccagcac cggcttcacc aatcccgaca cgctggaggc ctacgcggcg ttgagctgga
     2881 agtggctgca aggcaaggtg tcctatgtgg ttacaaatag cgcctggggt accgacgagg
     2941 ctgatggcac ctattacgcc gagctcaatg ccaacatccc gattgccgaa agcggcttta
     3001 ccgccaatct gcacgtcggc cgccaggaat tcgctggcag caatgaaggc gtggacaacg
     3061 acaactacag ctacaccgat tggaaagctg gcgtgaccaa gtccttcagc aatggtgtca
     3121 atgtcggcgg ctattacacc accgccaaca ccagggattc cttctggacg atcgacaaca
     3181 ggaacctcgg tgacgacacg tttactgtgt tcgtgcagaa ggtgttctaa aggcaacgaa
     3241 aatgcggcgg ggtagaggaa tgctctgtcc cgtcgcttcc aatactctca agtcaggaga
     3301 ctttcatgaa attcgtcact gccatcatca agccgttcaa gcttgacgag gtgcgtgagg
     3361 ctctttccgc catcggcgtg cagggcatca ccgttaccga ggtcaagggt ttcgggcggc
     3421 aaaagggcca c
//
LOCUS       DAOMFA010000161         3361 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_3980_length_3361_cov_5.526316, whole genome shotgun sequence.
ACCESSION   DAOMFA010000161 DAOMFA010000000
VERSION     DAOMFA010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 3361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3361
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3980_length_3361_cov_5.526316"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..458)
                     /locus_tag="PKW44_07615"
     CDS             complement(<1..458)
                     /locus_tag="PKW44_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018412145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HQR51488.1"
                     /translation="MTDTPPTPQNDDLPAVEVVSESKRSLSLVWIIPLVAAVIGGWLV
                     VKTILEQGPTITIQFENAYGLEAGKTHIKYKDFNVGLVNDIVLSEDRNHFIVTAQLVK
                     EAKPWLVEDTKFWVVRPRFSGGQVSGIGTLLSGSYIGIDPGKSSKEQLKHT"
     gene            complement(451..1083)
                     /locus_tag="PKW44_07620"
     CDS             complement(451..1083)
                     /locus_tag="PKW44_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraquat-inducible protein A"
                     /protein_id="HQR51489.1"
                     /translation="MSRLPITAASLGLLSCHDCDLLSKPTDGQVAGVCPRCGAHMHLR
                     KPNSLTRTWALLITAAILYIPANLLPVMQAGSMFGSQKDTIFSGVVYLWNSGSAPIAL
                     LIFFASITVPLAKLIALTYLLISVHRHSQKRPLQRTQIYRLAEFVGRWSMIDVYVVAL
                     LVTLVQFGGFATIEPGPGAIAFGGVVVLTMIAAMSFDPRLIWDPIKEKHD"
     gene            complement(1080..1715)
                     /locus_tag="PKW44_07625"
     CDS             complement(1080..1715)
                     /locus_tag="PKW44_07625"
                     /inference="COORDINATES: protein motif:HMM:NF016301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraquat-inducible protein A"
                     /protein_id="HQR51490.1"
                     /translation="MSEPLAVSSLIACHECDLLHRRMVLPQGGVARCTRCGAELYANP
                     RDSLNRALALTLAAAILFATVNFFPIVDLDAQGIVTKATLPGSALALHDTGMTSVALL
                     VFTTTVLVPALLFAAMLYLLIPYKFGRTPPMLPQVLRAVHMLKHWGMVEVYLLGVLAA
                     LVKLGHLAIIKPGPSLWLLAVLVFVLAAAVSAFNAHDFWHEGESSASDLSA"
     gene            complement(1783..2298)
                     /locus_tag="PKW44_07630"
     CDS             complement(1783..2298)
                     /locus_tag="PKW44_07630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR51491.1"
                     /translation="MKKAVAVVSLALSAALAAPVYAADQADQSAQTAEAAQPTKEQQK
                     IALDKLKADKKFITSENLALTEAEAKGFWPIYDEYQDGLEKLNQRTLDAIGEYADLYR
                     NNTMTDAKALVLVNKFLAIESDELALKKSTLPKVQKVLPGIKEARYFQIENKLRAIVK
                     IKLAEAIPLVK"
     gene            complement(2436..2810)
                     /locus_tag="PKW44_07635"
     CDS             complement(2436..2810)
                     /locus_tag="PKW44_07635"
                     /inference="COORDINATES: protein motif:HMM:NF023197.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 family protein"
                     /protein_id="HQR51492.1"
                     /translation="MRSKVGARVPLVDQAGPGVVRVRVAISSVTAEAAALKAYQYIPL
                     AYLVHKADEAVTGTTMVSMVFLEAELNDSVTGELLAEAVKRGDGATVKEDVQVTAADV
                     TPLIDRWAQLAADLIASRLGGK"
     gene            complement(2824..3096)
                     /locus_tag="PKW44_07640"
     CDS             complement(2824..3096)
                     /locus_tag="PKW44_07640"
                     /inference="COORDINATES: protein motif:HMM:NF023197.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 family protein"
                     /protein_id="HQR51493.1"
                     /translation="MIKTILSVIVLALLLASCATKGDVSGEKNSGFLESYSILKDEKD
                     ADGNPVRRWISSKLNAQNYQKVLIEPVIYFPPFRHGRCRDAGGGQA"
BASE COUNT          717 a          985 c          986 g          673 t
ORIGIN      
        1 gtatgcttta attgctcctt gcttgacttg ccaggatcta tgccgatgta ggaaccggac
       61 agcaaggtgc cgatccccga aacttgcccg cccgagaaac gcgggcgcac cacccagaat
      121 ttggtatctt cgaccagcca aggcttggct tccttgacca gttgcgcggt gacgatgaaa
      181 tgattgcggt cttccgacag tacgatgtca ttgaccagac caacgttgaa atccttgtac
      241 ttgatatgcg tcttgcctgc ctccagccca taggcgttct cgaactggat ggtgatggtg
      301 gggccctgtt ccaggatggt cttgaccacc agccagccgc cgatcactgc cgccacgaga
      361 ggaatgatcc agaccaagga tagcgaacgc ttgctttcgg agacgacttc cactgcggga
      421 aggtcatcgt tctggggggt ggggggcgta tcagtcatgc ttttccttta ttgggtccca
      481 gatcaggcgg gggtcgaacg acatggcggc gatcatggtc agcaccacca ccccgccgaa
      541 ggcaatggcg ccaggcccgg gttctatggt ggcgaagccg ccgaactgta ccagcgtcac
      601 cagcagcgca accacgtaaa catcaatcat ggaccagcgc ccgacaaact cggccagtcg
      661 gtaaatctgg gtccgctgca acggccgttt ctgcgaatgg cgatgtaccg agatgagaag
      721 gtaagtcaac gcaatcaact tggcaagcgg caccgtgatg ctggcgaaaa agatcagtag
      781 cgcaatgggc gccgaacccg aattccacag gtagaccacg ccgctgaaga tggtgtcttt
      841 ctgcgaaccg aacatcgatc cggcctgcat caccggcaac aggttggccg ggatgtaaag
      901 gatcgcggcg gtaatcagca aagcccaggt acgcgtgagg ctgttcggtt tgcgcagatg
      961 catgtgcgcg ccacagcgcg ggcatacccc cgccacctga ccatctgtcg gcttgctgag
     1021 caggtcgcaa tcgtgacaag aaagcaggcc cagcgaggcg gcggtaatgg gcagccgact
     1081 catgcagaaa gatcgctggc gctggattcg ccttcatgcc agaagtcgtg cgcgttgaaa
     1141 gcggaaacag cagcagccag aacgaatacc agtaccgcca gcagccacag cgaagggccg
     1201 ggtttgatga tcgccaagtg gccaagtttg accagtgccg ccagcacgcc cagcaggtag
     1261 acctcgacca tgccccagtg cttgagcata tgaaccgcgc gcagtacctg cggcagcatt
     1321 ggcggcgtcc gcccgaattt gtatggaatc aagaggtaca gcatggcagc aaagagcagc
     1381 gcgggcacca gcacggtcgt agtgaatacc agcagggcta cactggtcat gcccgtgtcg
     1441 tgcagtgcca gggccgagcc cggcagagtc gccttggtga cgatgccttg ggcgtcgaga
     1501 tcgacgatgg gaaagaaatt gaccgtggcg aacaggatgg ccgcggccag agtcagcgcc
     1561 agcgcgcggt tgaggctgtc gcgcggattg gcatacagtt ccgccccgca gcgtgtgcat
     1621 cgcgccacgc ccccttgggg caagaccatc cggcggtgca gcaggtcgca ttcgtggcag
     1681 gcgatcagcg acgatacggc aaggggctca ctcatacagc acccacccag agattgcctt
     1741 ccctgacggc tggccgatgc agggaagggt cggtttcatt gtttacttaa ccagcgggat
     1801 cgcttcggcc agcttgatct tgacgatcgc gcgcagcttg ttctcgatct ggaaatagcg
     1861 cgcctccttg atccccggca gcaccttttg caccttgggg agggtgctct tcttcagcgc
     1921 cagctcatcg ctttcgatgg ccaggaactt gttgaccagc accagcgcct tggcgtcggt
     1981 catggtgttg ttgcgataaa gatcggcata ttcgccgatc gcgtccagcg tacgctggtt
     2041 cagcttctcc aggccatcct ggtattcgtc gtagatcggc cagaagccct tggcctcggc
     2101 ttcggtcagg gccaggttct ccgaggtgat gaatttcttg tccgctttga gcttgtccag
     2161 agcgatcttt tgctgctcct tggtcggctg ggcagcctcg gctgtctgag cggattgatc
     2221 ggcctggtca gcggcgtaga cgggcgctgc cagagcggcg ctgagcgcca gggatacaac
     2281 tgcaactgct ttcttcatac tttttctcct agttgacatt gacaaaaaac cggttcgaca
     2341 gcgggatgcg cctgcataag cgcatcccgc acaaactgat cagatgtgca tgttagcagg
     2401 caccgcgtgc gctgcatacc accacgcctg cgccgttatt ttccgcccag cctgctggcg
     2461 atcaaatccg cagccagttg cgcccagcgg tcgatcaggg gcgtcacatc ggcggcggtc
     2521 acctgcacat cctccttcac cgtggcgcca tcgccccgct tgacagcttc ggccagcagt
     2581 tcgcccgtca cgctgtcatt cagctcggct tccagaaaca ccatgctcac catcgtggtg
     2641 ccggtgacgg cttcatccgc cttgtgtacc agataggcca gcggaatgta ctgataggcc
     2701 ttgagtgcgg cagcttcggc ggtcaccgaa gaaatagcga cgcgcacgcg caccacgccc
     2761 ggccccgcct ggtcgaccag cggcacccgt gcccccacct tgctgcgcag cgtctgctcc
     2821 agattacgcc tgaccgcctc cagcgtcgcg gcatctgccg tggcggaacg gagggaaata
     2881 aatcaccggc tcgatcagga ccttctggta gttctgcgca ttgagtttgg aactgatcca
     2941 gcgtcgcact ggattaccat cggcatcctt ttcgtccttg aggatcgagt aactttccag
     3001 gaacccggaa ttcttttctc ccgacacatc tcccttggta gcacaggaag cgagcaacag
     3061 cgccaagacg atgacgctga gaatggtttt gatcatggta atgctccttg ggtaaagtga
     3121 aaacgctcga gaccaatatg aatagccaaa tgaggcgatc cgggcattgc tatgcacatg
     3181 gcaatgcaac agaatactga cgaggcgatt atatagtagg aggaaataaa attgcgctgg
     3241 acgttataac gacaaaaggc acccaaaagt gccttttgtc gttaattatg gtggcggaac
     3301 acggaactga ccccgcgtcc gtaatctgaa ccagagagta acacatcggc cttgaggccg
     3361 t
//
LOCUS       DAOMFA010000162         3355 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_3995_length_3355_cov_5.001515, whole genome shotgun sequence.
ACCESSION   DAOMFA010000162 DAOMFA010000000
VERSION     DAOMFA010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 3355)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3355)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3355
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3995_length_3355_cov_5.001515"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..311
                     /locus_tag="PKW44_07645"
     CDS             <1..311
                     /locus_tag="PKW44_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR51494.1"
                     /translation="ELLLNRLVLTTGARENLGFRDAAALVDTIMADHGRDPGDWLLLR
                     RYERSRKSDILSMQVVTDGLQRLFNNDLPGLGTLRNSGLAAVNRSGWLKKQLMAQAMI
                     "
     gene            347..1078
                     /locus_tag="PKW44_07650"
     CDS             347..1078
                     /locus_tag="PKW44_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HQR51495.1"
                     /translation="MWRKCAVGLILTLAATLAVADEASVRKHFEAAYPNVDVKSVTKT
                     PYAGLYEVVAGEEIVYTDEGLNYLFLQGDLVDVKSRRSLTGPRRDELEENKTQKTAID
                     FSALPLDKAVKVVRGDGSRKVAVFSDPDCPYCQKLEREALANITDVTVYTFLYPLAGH
                     ADAPRKAKAIWCAPDRAKAWEDWMLRGQLADGSTSCETPLEPIRALGFKLDVDATPTL
                     FFPSGRQLRGAYPADTIEKELQASR"
     gene            1245..1733
                     /gene="secB"
                     /locus_tag="PKW44_07655"
     CDS             1245..1733
                     /gene="secB"
                     /locus_tag="PKW44_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HQR51496.1"
                     /translation="MAEEQQQPAAQEQDQQPVFGIEKIYVKDLSLEIPNAPQVFLQRE
                     TPQVGIELQNSANPVGEGLYEAVLTITVTSKLEDKTVFLVEVAQAGIFQVRNVPEESL
                     EGIFGVTCPNILFPYARETISDVVTRAGFPPVLLNPINFEALFVQQKQQAQQAGASET
                     TH"
     gene            1753..2163
                     /locus_tag="PKW44_07660"
     CDS             1753..2163
                     /locus_tag="PKW44_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HQR51497.1"
                     /translation="MALLPATAWALDYRSVSAEKAILYDAPSTQGKKLFIVRQYYPVE
                     IIVDLGEWIKVRDSRGELAWIEARNLAAKREVEVTAAQAEVRESANADARLVFLADKA
                     VVLELLEPASNGWAKVRHRDGLTGYIQTSKVWGL"
     gene            2173..3162
                     /locus_tag="PKW44_07665"
     CDS             2173..3162
                     /locus_tag="PKW44_07665"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011380470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HQR51498.1"
                     /translation="MKITVLGAGAWGSALAIHLARSHPVVMWARNAEHVAAMQATRSN
                     PRYLGDYAFPEALQVQGDFQTAIRDADLILSVVPTAGFRQALREIVAGGCKAPVVWAN
                     KGLERGTAKLMPEVAAEELPRSQLRGLLSGPSFASDLAKELPTAMTLAAADNDFAQTA
                     AAAMHGGNVRVYTSTDVIGASVGGALKNVMAIAAGINDGMGFGNNARAALITRGLAEI
                     TRFGMALGGRLETFMGLAGAGDLVLTCTGDASRNRTVGLRLATGETLEQIVSSLGHVA
                     EGVPTTREVHDLAQKMGVDMPITNEVNRVLSEGLSPRVAVENLLSREQKAEHY"
BASE COUNT          728 a         1083 c          990 g          554 t
ORIGIN      
        1 aagagctcct cttgaaccgg ctcgtgctga cgaccggcgc gcgcgagaac ctcggcttcc
       61 gcgacgcggc ggcgctagtc gataccatca tggccgacca cgggcgcgac cctggagatt
      121 ggctgctgct gcgccgctac gagcgttcgc gcaaaagcga catcctgtcc atgcaagtcg
      181 tcacggatgg cctgcagcgc ctgttcaaca acgacctgcc ggggctgggg acgctgcgca
      241 acagcggcct ggccgcagta aaccgcagcg gctggctgaa gaagcagctg atggcgcagg
      301 ccatgatcta gccattcatc acgcaacaaa aacctgagga aaacgcatgt ggcgcaagtg
      361 tgcagtcgga ctgattctga cgttggcggc aacgctcgca gtcgcggacg aagcctcggt
      421 cagaaagcac tttgaagcgg cctaccccaa cgtcgatgtg aagagcgtga caaaaacccc
      481 gtatgccggc ctgtacgagg tagtggcggg cgaggaaatc gtctataccg acgaagggct
      541 gaattacctt tttctgcaag gcgatctggt cgacgtcaaa tcccggcgca gcctcaccgg
      601 cccgcgccgg gatgaactgg aagaaaacaa gacacaaaaa accgccatcg acttcagcgc
      661 cttgccgctg gacaaagcgg tcaaggtcgt ccgcggcgac ggcagccgca aggtggccgt
      721 gttctccgat ccagactgcc cttactgcca gaaactggaa cgcgaagcgc tggccaacat
      781 caccgatgtc acggtttaca cctttcttta tccgctggcc ggccatgccg atgcgccgcg
      841 caaggccaag gccatctggt gcgcgccgga tcgcgccaag gcttgggaag actggatgct
      901 gcgtgggcag ctggcagacg gttccaccag ctgcgagacg ccgctggaac cgatccgcgc
      961 gctgggattc aagctggacg tggatgccac gcctaccctg ttctttccca gcggcaggca
     1021 gctcaggggc gcctacccgg cggacacgat cgaaaaagaa ctgcaagcaa gcaggtagga
     1081 gcgccgcctc ggcgcgataa gccaaaccat tatcggggcg aaggcgcccc tcctaccaca
     1141 cgactgttgc gtcgtcctca tccccggctt tcactcccat acggttaaag gctagaatag
     1201 cgcctttcca taaacccctt attcaccctc ttttaggaac caccatggca gaagaacagc
     1261 agcaacccgc agcacaggaa caggatcagc aacccgtatt cggcatcgag aagatctatg
     1321 tcaaggacct gtcgctggaa atccccaatg ccccgcaagt gttcctgcag cgcgaaaccc
     1381 cgcaggtcgg catcgagctg caaaacagcg ccaacccggt aggcgagggc ctgtacgaag
     1441 cggtgctgac cattaccgtc acctccaaac tggaagacaa gaccgtgttt ctggtggaag
     1501 tggcccaggc cggcattttc caggtgcgca acgtacccga ggaaagcctg gaaggcattt
     1561 tcggcgtcac ctgccccaac atcctgttcc cttacgctcg cgaaaccatc tccgacgtgg
     1621 tcacgcgcgc cggcttcccg ccggtgctgc tcaacccgat caatttcgag gcgcttttcg
     1681 tccagcagaa acagcaggcg cagcaggccg gtgcaagcga aacaacgcac taagccactc
     1741 cttgcgtttc tggtggcgct gctgccggca accgcatggg cgctggacta tcgctcggtg
     1801 tcggcggaaa aggccatcct ctacgatgcg ccttccaccc agggcaaaaa actgttcatc
     1861 gtccgtcagt actacccggt cgaaatcatc gtcgaccttg gcgagtggat caaggtgcgt
     1921 gacagccgcg gcgaattggc atggatcgag gccaggaatc tcgccgcaaa acgtgaagtc
     1981 gaggtcacgg cagcgcaggc ggaagtgcgc gaaagcgcca acgcagacgc gcgcctggtg
     2041 ttcctcgccg acaaggctgt ggtgctggaa ctgctcgaac ctgccagcaa cggctgggcc
     2101 aaggttcgcc accgcgacgg cctgaccggt tatatccaga cctccaaggt ctggggctta
     2161 taagggggtt ccatgaaaat caccgttctt ggcgccggcg cctggggtag cgcgctggcc
     2221 atccatctgg cgcgcagcca cccggtcgta atgtgggcac gcaacgcaga gcacgtcgcc
     2281 gccatgcagg cgacgcgatc caacccgcgt tatctcggcg actatgcctt tcccgaagcc
     2341 ctgcaggtgc aaggcgactt ccagaccgcg atacgcgacg ccgacctgat tctctccgtg
     2401 gtacccaccg ccggcttccg ccaagcgctg cgcgagatcg tcgccggcgg ctgcaaagcg
     2461 ccggtggtgt gggccaacaa gggcctggaa cgcggcaccg ccaaactgat gcccgaggtg
     2521 gccgccgagg aactgccgcg cagccagttg cgcggattgc tttccggccc cagctttgcc
     2581 agcgacctgg ctaaggaact gccgacggcg atgacgctgg ctgctgccga caacgacttc
     2641 gcacagaccg ccgccgccgc catgcacggc ggcaatgtgc gcgtctacac cagcaccgac
     2701 gtgatcggcg cttccgtcgg cggcgcgctg aagaacgtga tggccattgc cgccggcatc
     2761 aacgacggca tgggcttcgg caacaacgcc cgcgccgcgc tgattacgcg cgggctggcc
     2821 gagatcaccc gcttcggcat ggcgctgggc gggcggctgg aaaccttcat gggactggcc
     2881 ggcgccggcg acctggtact cacctgcacc ggcgacgcct cgcgcaaccg caccgtcggc
     2941 ctgcgcctcg ccaccggcga aacgctggag caaatcgtat cctccctcgg ccacgtcgcc
     3001 gaaggcgtcc ccaccacacg cgaagtacac gacctggcgc agaaaatggg cgtagacatg
     3061 cccatcacca acgaagtcaa ccgagtgctg tccgaaggac tgtcaccgcg ggtcgcggta
     3121 gaaaacctgc ttagccgcga gcaaaaggct gagcactact gagaccatct ggaaaaactc
     3181 tgttaaggaa agaagatgtc aaaagtccgc ttattatttc ccttcctcgc acttacactt
     3241 caattactag gtgggtgcgc atctgtgacc gggtcaccta atcaaagcgt ctctttgcaa
     3301 tcacgagaac agaatggtaa agaagtaaca ggagcaacct gtgaactcac aaaca
//
LOCUS       DAOMFA010000163         3319 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_4063_length_3319_cov_5.674939, whole genome shotgun sequence.
ACCESSION   DAOMFA010000163 DAOMFA010000000
VERSION     DAOMFA010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 3319)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3319)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3319
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4063_length_3319_cov_5.674939"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1419)
                     /gene="dnaG"
                     /locus_tag="PKW44_07670"
     CDS             complement(<1..1419)
                     /gene="dnaG"
                     /locus_tag="PKW44_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HQR51499.1"
                     /translation="MAIPESFIQDLLNRVDIVDVIDGSVPLKKAGANYAACCPFHNEK
                     TPSFTVSPTKQFYHCFGCGAHGTALGFLMEYQGLGFVEAVQELAKRVGMVVPQETRSV
                     QDIAAKKAAVGMQEALQLALNYYRGELKKSEKAIAYLKGRGLSGEVAARFQIGYAPPG
                     WQNLSEVLPDYQSETLTQAGLVIANEQGRRYDRFRDRIMFPIHDQKGQVIGFGGRVLG
                     GESDDAGPKYLNSPETPLFQKGHELYGLFLARRAIRESGRALVVEGYMDVVALAQYGI
                     GYAVAALGTATTPYHITKLMRQTDDIVFCFDGDAAGRKAAWRAAMNSLPALTDGLKLS
                     FLFLPPEHDPDSYVRAYGKEAFEQAMQGALPLSQYLIEKLSEENDLQSQEDRVRFINE
                     AEPILKQLQAPRLGLLLRKRVADLGGMSLEELEGALGLKQAASGRAAQPARSGRKPPT
                     LVRRLLHILLLRPQLAAQLDVQL"
     gene            complement(1494..1940)
                     /locus_tag="PKW44_07675"
     CDS             complement(1494..1940)
                     /locus_tag="PKW44_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HQR51500.1"
                     /translation="MSLKVQITEDMKSAMRAKDAPRLGAIRLLQAAIKQREVDERIEL
                     DDTQVIEAIEKMLKQRRDSISQYEAASRHDLADVEKFEVAILQTYLPQALTADEIAAL
                     LDKVVAETGASGIKDMGKVMAAVKPLVVGRADMGSISGLIKARLSA"
     gene            complement(1953..2165)
                     /gene="rpsU"
                     /locus_tag="PKW44_07680"
     CDS             complement(1953..2165)
                     /gene="rpsU"
                     /locus_tag="PKW44_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HQR51501.1"
                     /translation="MTTVRVKENEPFDVALRRFKRIMEKTGLLTELRAREFYEKPTWE
                     RKRKAAAAVKRHYRRIRNQTLPPKLF"
     gene            2260..3273
                     /gene="tsaD"
                     /locus_tag="PKW44_07685"
     CDS             2260..3273
                     /gene="tsaD"
                     /locus_tag="PKW44_07685"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HQR51502.1"
                     /translation="MIVLGIETSCDETGIALYDTEKGLLAHALHSQIKMHAEYGGVVP
                     ELASRDHIRRVLPLTEKVLQDAGLALQDIDAIAYTQGPGLAGALLVGTSVAEALAFTL
                     NIPAIGVHHLEGHLLAPLLEENPPAFPFIALLVSGGHTQLMRVSGIGEYELLGDTLDD
                     AAGEAFDKTAKLLGLEYPGGPAVSRLAEQGTPGRYKLPRPMLNSGNLDFSFSGLKTAV
                     LTAVNEAPKDGTAHADLARAFQDAVVGVLTVKCVAALEQTGLERLIVSGGVGANKQLR
                     AALDAGARKNGFDVSYPRLEFCTDNGAMIAFAGAMRLKNLQPGNHAFTVRPRWDLAEL
                     TAP"
BASE COUNT          601 a         1067 c         1001 g          650 t
ORIGIN      
        1 cagctgcacg tcgagctgcg cagccagctg cggacgcaga agcagaatat ggagaagacg
       61 ccgcaccagc gtcggcggtt tgcggccgct gcgcgccggt tgcgcggcgc gtcccgacgc
      121 cgcctgtttc agtccgagcg cgccttccaa ctcttccagc gacattccgc ccaaatctgc
      181 cacgcgcttg cgcagcagca agcccagccg cggcgcctgg agctgcttca ggatcggttc
      241 cgcttcattg atgaagcgca cccggtcttc ctgtgattgc agatcgtttt cctcgctcaa
      301 cttttcgatc aggtactgtg acagcggcaa ggcgccctgc atcgcctgtt cgaatgcttc
      361 cttgccatag gcacgcacgt agctgtccgg gtcgtgctcc ggcggcagga acaggaagct
      421 cagcttcaaa ccgtcggtca gtgccggcag gctgttcatc gccgcacgcc acgccgcctt
      481 gcgtcccgcc gcgtcgccgt cgaagcagaa cacgatgtca tcggtctgcc gcatcaattt
      541 ggtgatgtgg tagggcgtag tcgccgtgcc aagcgccgcg acggcataac cgataccata
      601 ttgcgccagc gccaccacgt ccatgtagcc ttcgaccacc aaagcgcgcc cggattcgcg
      661 gatggcgcgg cgcgccagga acaggccgta cagctcgtgt cctttctgga acagcggggt
      721 ttccggcgag ttgaggtatt tcggcccggc atcgtcgctc tcgccgccca gcacgcgccc
      781 gccgaagccg atcacttggc ctttctggtc gtggatggga aacatgatgc gatcgcggaa
      841 gcggtcgtag cgccgcccct gctcgttggc gatgaccagc cctgcctggg tcagcgtttc
      901 ggactggtaa tccggcaata cttcggacag attctgccag ccgggcggcg catagcctat
      961 ctgaaagcgc gccgcgactt cgccgctcag gccgcgcccc ttgaggtagg caatcgcctt
     1021 ttccgatttt ttcagttcgc cgcggtaata gttaagtgcc agctgcagcg cctcctgcat
     1081 gccgaccgcg gcttttttcg ccgcgatatc ctgcacgctg cgcgtttcct gcggcactac
     1141 catgccgaca cgcttggcca gctcctgcac cgcctcgacg aagcccagac cctggtattc
     1201 catcaggaag cccagagccg tgccatgtgc gccgcagccg aagcagtggt agaactgctt
     1261 ggtcgggctg acggtgaagg acggggtttt ctcgttgtgg aacgggcagc aggcggcgta
     1321 gttggcaccg gcctttttca gcggcacgct gccgtcgatc acgtcgacga tgtcaacgcg
     1381 gttcaacaag tcttggatga aggattccgg gatcgccatg tcgctaggga atataaaaca
     1441 aaaaaggagc cttacgctcc tttttgttgg tgtctgccat ccagcgttgc gagctaggca
     1501 ctcaacctcg ccttgatcag gcccgagatg ctgcccatat ctgcgcgccc caccaccagc
     1561 ggcttcacgg cggccatcac cttgcccatg tccttgatgc cactggcacc ggtctcggcc
     1621 accaccttgt ccagcaaggc ggcaatctcg tcagccgtca gcgcttgcgg caaataggtt
     1681 tgcagtatgg cgacctcgaa cttttcgaca tcggccagat cgtggcggct ggcagcttcg
     1741 tactggctga tcgaatcgcg gcgctgcttg agcatctttt cgatagcctc gatcacctgc
     1801 gtgtcatcca gttcgatgcg ctcatccact tcgcgctgct tgatggcagc ctgcagcaac
     1861 cggatggcac ccaggcgcgg ggcatccttg gcgcgcatgg cggacttcat gtcctcggtg
     1921 atctggactt tcagcgacac aacgaaagat ttttagaaca gcttgggcgg cagggtctga
     1981 ttgcggatac ggcgatagtg gcgcttgacg gcggcggcgg ccttgcgctt gcgctcccag
     2041 gtcggcttct cgtagaactc gcgggcgcgc agttcggtca gcaggccggt cttttccata
     2101 atgcgcttga agcggcgcag ggcaacgtca aacggctcgt tctcttttac acgtacggta
     2161 gtcattgaaa tcttctctcc taaagggcga tttaaaagtc ggaggcgacg aaaaagtttg
     2221 ctattctagc cagctattta ccaacaatca atgactttca tgatcgtctt aggcatcgaa
     2281 acctcctgcg acgaaaccgg catcgcgctc tacgatacgg aaaagggtct gctggcacac
     2341 gcgctgcact cgcaaatcaa gatgcacgcc gaatacggcg gcgtggtgcc ggaactcgct
     2401 tcgcgcgacc atatccggcg cgtgctgccg ctgacagaaa aggtattgca ggatgccgga
     2461 ctggccttgc aggacatcga cgccatcgcc tatacccagg ggccggggct ggccggtgcc
     2521 ttgctggtcg gcaccagcgt ggccgaagcg ctggccttca ccttgaacat tcccgccatc
     2581 ggcgtacacc atctggaagg ccacctgctc gccccgctgc tggaagaaaa cccgccggca
     2641 ttccccttca tcgcactgct ggtgtccggc ggccataccc agttgatgcg tgtgtccggc
     2701 atcggcgaat acgaactgct gggcgatacg ctggacgacg ccgccggcga agccttcgac
     2761 aagaccgcca aactgctcgg cctcgaatat cccggcggcc cggcggtttc caggctggcg
     2821 gaacagggca cgccgggacg ctacaagctg ccgcggccga tgctcaatag cggcaacctc
     2881 gatttcagct tcagcggact caagaccgcg gtactgacgg cggtaaacga agcgccgaag
     2941 gacggcacgg cccacgccga cctcgcccgc gccttccagg acgccgtggt aggcgtgctg
     3001 accgtcaaat gcgtagccgc gctggaacaa accgggctgg agcgcctgat cgtctccggt
     3061 ggcgtcgggg ccaacaagca actgcgggcg gcgctggatg ccggcgcgcg gaaaaacggc
     3121 ttcgacgtct cctacccgcg cctggagttc tgcaccgaca acggcgccat gatcgccttc
     3181 gctggcgcca tgcggctgaa gaatttacag cccggcaacc atgccttcac ggtcagaccg
     3241 aggtgggatc tggcggaatt gaccgccccc tgattattgc ggggggtggg cgcccggctt
     3301 ggggtttgcg aacccggcc
//
LOCUS       DAOMFA010000164         3244 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_4201_length_3244_cov_5.354029, whole genome shotgun sequence.
ACCESSION   DAOMFA010000164 DAOMFA010000000
VERSION     DAOMFA010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 3244)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3244)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3244
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4201_length_3244_cov_5.354029"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..464
                     /locus_tag="PKW44_07690"
     CDS             <1..464
                     /locus_tag="PKW44_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018230778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HQR51503.1"
                     /translation="IVDMPPGTGDIQLSLAQKVPVTGAVIVTTPQDIALIDARKGLKM
                     FEKVNIPILGIVENMSIHICSQCGHEEHIFGTGGGEKMCADYGVDFLGSLPLEMAIRE
                     MTDGGKPTVVGAPDSRTANIYRGIARRIAVKIAQKAKDMTSKFPNIVVQNT"
     gene            887..1384
                     /locus_tag="PKW44_07695"
     CDS             887..1384
                     /locus_tag="PKW44_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR51504.1"
                     /translation="MRIIAPLLLFIALSGCASYDGSGLKPGSSTTADVVQAMGTPAMR
                     WNEPGGGELLAFPRGPSGTETFMVRIAANGILASKENVLDMRHFAQIQAGMTEQEVLR
                     VIGPPFPGWTATYPARSELVWEWRYCSEYREASRFDVIFDSATGKVKTTQSYVESIGR
                     GHPRC"
     gene            complement(1451..1714)
                     /locus_tag="PKW44_07700"
     CDS             complement(1451..1714)
                     /locus_tag="PKW44_07700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR51505.1"
                     /translation="MWAGVAALVVALVGGLGYWGWSNKGASEDSARKLADVEQRRIAA
                     ERTAEAAEIAAAKELLDKHIAAEEAQAEAAARSRGSGSTALKR"
     gene            complement(1918..>3244)
                     /locus_tag="PKW44_07705"
     CDS             complement(1918..>3244)
                     /locus_tag="PKW44_07705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR51506.1"
                     /translation="HDGRRPSLDARRPTPAQPIAVSEATSVVPRGDRSSFDIRHKNPD
                     GSQMVISQKLMPDGTKKVSGFQQTEDLRRGTTNRVYADGHRVTVGKDYERLSMGSGPA
                     FVTNRNGLREAVLPDGRPAFQDRFTSFRDRDGSERRVIERTHYARWSYGRPQYVDRPF
                     IRLYDVGQIYGAPVAYYRPVRFAYAEYRPFYQRFAVPLVLASVVTVGAMAAVAFASQD
                     TGYDDPMALMGDMQIASGFEEGYGYSPTYGATQDYDTPEAVALRSQMAEVQRQVKTSV
                     KGDPSLAQQLGEVDLQASSLQVQQELGGAVPVQIPEEVRQQVRKQVRLSVAMHQNGRA
                     LALDDVLASGYAKIYLFQTAHPLNVGDLSSGGECFLMTGDLIGFSSLPAANSPFAEMK
                     VVASGANSCQTGGVVEVRLTDLQEMLNGFSERVEDNMKRVSACAPSGRC"
BASE COUNT          635 a         1004 c          955 g          650 t
ORIGIN      
        1 tgatcgtcga catgccgccg ggcaccggcg acatccagtt gtctctggcg cagaaagtgc
       61 cggtgaccgg tgcggtgatc gtcaccacgc cgcaggacat cgcgctgatt gacgcgcgca
      121 aggggctgaa gatgttcgag aaggtcaata tcccgatcct cggcatcgtc gagaacatga
      181 gcatccacat ctgctcgcag tgcggccacg aggagcacat ttttggcacc ggcggtggcg
      241 agaaaatgtg tgcggactac ggcgtcgatt tcctcggcag cctgccgctg gaaatggcga
      301 tccgcgagat gaccgatggt ggcaagccga ccgtggtcgg cgcgccggat tcgcgcacgg
      361 ctaatatata ccgcggaatc gcccgccgca tagcggtcaa gattgctcag aaggccaagg
      421 acatgacatc caagttcccc aacattgtcg tgcagaacac ctgatgaagt ggtgatttcc
      481 ttctggtccc cgcgcctttt cgcctcaagc tgaaatgagc ctgccgggcg ttgctgcggt
      541 caactgggga aagtggccaa atcgtggtcg gcccatgcag ggacatcagc ctgccacatc
      601 cgaattgcga cttgcggcgt ggtgcgtgtc gatctggcgg ctcagtccgg caaggtgaac
      661 atgacgctac tttgcagggg ggctgcaccg aatcgatact tccgcaaggt cttggtgagc
      721 cacttcgtga ttcatcgcta aaacggcatt ggcggcatgg aggcgtgggt aaaaacccgc
      781 aagctgggag taagccattg agtggggtag agaaaatttg ctctgaccac caaagcgcgg
      841 atatattttc actcgctatc tgaagccact tgaggaaagg tcgaccatgc gaatcattgc
      901 ccccctgctc ctgttcatcg cgctctccgg ctgcgcttcc tacgatggca gcggcctgaa
      961 acccggaagc agcaccacgg ccgacgtcgt ccaagcgatg gggacgccgg ccatgcgctg
     1021 gaacgagccc ggtggtggcg aactgctggc ctttccacgc ggccccagcg gaaccgaaac
     1081 cttcatggtc aggattgccg ccaacggcat cctcgcgagc aaggagaatg tgctcgacat
     1141 gcgtcatttc gcgcagatac aggcgggtat gaccgagcag gaggtgctgc gcgttatcgg
     1201 tcccccgttc ccggggtgga cggccaccta cccggcgcgt agcgaactgg tgtgggagtg
     1261 gaggtattgc agtgagtacc gcgaagcatc ccggttcgac gtgatcttcg actccgcaac
     1321 tggcaaggtg aagaccaccc agagctatgt cgaatcgatc ggtcgcggac atccgaggtg
     1381 ttgaacatcc gcgccaagcc gccaaggggc aaagtccggc ccctggcttg cacagtgact
     1441 tggcatgatc tcaccgctta agtgccgtac tcccggagcc ccgacttcgg gcagcagctt
     1501 ccgcttgggc ttcctcggcg gctatgtgtt tatccagaag ttctttggcg gccgcgatct
     1561 cggcagcctc cgctgttctt tctgcagcta tacggcgctg ctcaacatcg gccagcttgc
     1621 gtgcgctgtc ttcgctggct cccttgttac tccagcccca atagcccaga ccgcccacca
     1681 atgccacgac cagcgccgca acaccggccc acatcgctcg tttgcttcca gacggtgaag
     1741 ctttcggcaa gcccgatgca ggaacagcca tcgttggttg tgtcggttcg tcagtttgat
     1801 gcgcgccaca gtgcgtgcag aactttgtat caggctttat ggccttgcca caattcgtgc
     1861 aaaacatgtt gatcttctcc ctcggcagac tgtgatatcg ggatgggcag aatgccttta
     1921 gcagcgcccc gagggggcgc aggcgcttac ccgcttcatg ttgtcttcca cgcgctcgct
     1981 aaagccattg agcatctcct gcaagtccgt cagccgaacc tccaccactc cgccagtctg
     2041 gcaactgttg gcgccgctgg caaccacctt catttcagca aacgggctat tggcagccgg
     2101 caggctcgag aagccgatca ggtcgccggt catcagaaaa cactcgccac ctgaggacag
     2161 atcccccacg ttgaggggat gtgccgtctg aaacaggtag atctttgcat agccggaagc
     2221 tagtacgtcg tccagcgcca gggcccgacc gttttgatgc atggcgacac tgaggcgcac
     2281 ttgcttgcgc acctgttggc ggacctcttc gggaatctgc accggcaccg cgcctcccag
     2341 ctcctgctgg acctgcaagg aagacgcttg caaatccacc tctcccaatt gttgcgcaag
     2401 tgacggatcg cctttgacgc tggtctttac ctgccgttgc acctcagcca tctggctgcg
     2461 aagggccacc gcttcaggcg tatcatagtc ctgcgtcgcg ccgtaggttg gtgaatagcc
     2521 ataaccctct tcaaacccgg acgcgatctg catatctccc atcagcgcca tgggatcgtc
     2581 atagcccgtg tcttgggaag caaaggccac cgctgccatg gcgcccacgg tcaccacgga
     2641 cgcgagcacc aggggcaccg cgaagcgctg gtaaaaggga cggtactcag cataggcgaa
     2701 gcgaaccggc cgatagtacg caaccggcgc accgtatatc tgacccacgt cgtataggcg
     2761 aataaacgga cgatctacgt attgcggcct gccgtacgac cagcgtgcgt aatgcgtacg
     2821 ctcgatcacg cgccgttcgc tgccatcccg gtcgcgaaat gaggtaaagc gatcctgaaa
     2881 cgcaggccga ccgtccggca ataccgcctc acgcagcccg ttccggttcg tcacgaaggc
     2941 tgggccactg cccatggaaa gccgttcata gtccttgccc acggtcacac ggtggccgtc
     3001 ggcgtaaacg cggttggttg ttccgcgacg caaatcctcg gtctgctgga aaccgctcac
     3061 cttctttgtc ccatccggca tgagcttttg cgatatcacc atctgcgaac cgtcggggtt
     3121 tttgtgccga atgtcgaaac tgctgcgatc tccgcgtggc accacgctgg tggcttcgct
     3181 gacggcgatc ggttgtgctg gcgtcgggcg acgcgcgtcc aggctgggcc gacggccatc
     3241 gtgc
//
LOCUS       DAOMFA010000165         3239 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_4212_length_3239_cov_4.559987, whole genome shotgun sequence.
ACCESSION   DAOMFA010000165 DAOMFA010000000
VERSION     DAOMFA010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 3239)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3239)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3239
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4212_length_3239_cov_4.559987"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..640
                     /locus_tag="PKW44_07710"
     CDS             <1..640
                     /locus_tag="PKW44_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HQR51507.1"
                     /translation="KFVGFYSEDLGVFYLDILKDRLYTSCPDSRPRRAAQSALWHITQ
                     TLMRLIAPVLSFTAEEIWQTLNPSSETSVFEQEWHVLPEHGLGGKEILAWEDIINVRA
                     LVNKKIEEKREAGLVGSSLQSEIDIYAFDAIHHPLSRLGDDLRFVLITSRATLHHRAG
                     SGVGIDVTPSVHQKCERCWHYRADVGAHADHPHICGRCVSNLYGDGEPRQFA"
     gene            646..1098
                     /gene="lspA"
                     /locus_tag="PKW44_07715"
     CDS             646..1098
                     /gene="lspA"
                     /locus_tag="PKW44_07715"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HQR51508.1"
                     /translation="MRNWLGLSAVVIVLDLTTKHLIQSAFAYGERVSVTSFFDLVLFY
                     NEGAAFSFLAAAGGWQRLFFSAFAVIASVVIIHLLRKHPGQKLFCLALALILGGALGN
                     LYDRLTLGYVVDFLYFHYQAWYWPAFNVADSAISVGVVLLLWDGLKKP"
     gene            1150..1791
                     /locus_tag="PKW44_07720"
     CDS             1150..1791
                     /locus_tag="PKW44_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019899536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase-like oxidoreductase"
                     /protein_id="HQR51509.1"
                     /translation="MPDWKKLIEGKIALAKRGLKPGASSSHARVPAGQTLVTNFPVLH
                     ISEPPRLTSADWKLRVYGLVENALSLDWQAFRQLPQIKDVSDFHCVTRWSKLDVEWEG
                     VRASEVLALAMPLDTAKYVTLHSYDGYTTNLPLEALLDEDVIVAHSVLGQPLSADHGG
                     PVRLVVPKRYAWKSAKWLKAIELHAEDRPGFWEERGYHNEADPWKEERFSGNW"
     gene            complement(1920..2495)
                     /locus_tag="PKW44_07725"
     CDS             complement(1920..2495)
                     /locus_tag="PKW44_07725"
                     /inference="COORDINATES: protein motif:HMM:NF023273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HQR51510.1"
                     /translation="MKNPLISALLALAVSGPALADDLQEKYLKDSRETAQEFMLKLGT
                     MLKQQIEVGGAENAIGVCKQYAPALAAEYSGDNRAVKRVSLKPRNPALGTPDTWEKQM
                     LESFDNELSGGKPIASMEKNTVVETPDGRWFRYMKAIPTQPMCLQCHGQPYQISDGVK
                     ALLAKDYPDDQATGYSAGSIRGAVSIKWKMN"
     gene            complement(2557..3102)
                     /locus_tag="PKW44_07730"
     CDS             complement(2557..3102)
                     /locus_tag="PKW44_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013648640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HQR51511.1"
                     /translation="MSHALEECPSLLLVDDDDTFRQVLAPALKKRGFDVSTAADVAQA
                     LALAEANPPEYAVVDLKMPGQSGLVLVKRLKELDAATRIVMLTGFASIATAVEAVKLG
                     ATHYLAKPADADEIVAAFYRVEGDAAAPLNTSPLSVERLEWEHIQRILAEHDGNISAT
                     ARALDMHRRTLQRKLGKNPKK"
BASE COUNT          613 a          932 c          988 g          706 t
ORIGIN      
        1 gaagttcgtc ggtttctact cggaagacct gggcgttttt taccttgata tccttaaaga
       61 ccgtctctat acctcctgtc ccgattcccg cccgcgccgc gccgcgcaaa gcgcgttgtg
      121 gcacatcacg cagacattga tgaggctgat cgcgccggta ttgagcttta ccgccgagga
      181 aatctggcag acgctgaacc ccagcagcga gaccagcgta ttcgagcagg aatggcatgt
      241 gctgccagaa catgggcttg gaggcaaaga aattctggcc tgggaagaca tcatcaatgt
      301 gcgtgcactg gtgaacaaaa aaatcgagga aaaacgcgag gctggcttgg taggttcttc
      361 tctccaatcc gagattgata tttacgcttt cgatgcaatt catcatccgc tttcccgact
      421 aggggatgat ctgcgttttg tattgattac ttccagagcg acactgcatc atcgggcggg
      481 tagtggagta gggatagacg tgactcccag tgtccaccag aaatgcgagc gctgctggca
      541 ctaccgtgcc gatgtcgggg cgcatgcaga ccatccgcat atttgcgggc gctgcgtgag
      601 caacctgtac ggcgacggcg agccgcgcca gtttgcatga ggggcatgcg taactggctg
      661 gggctgagcg cggtcgtcat cgtgctcgat ctcaccacca agcatctgat ccagtcggcg
      721 tttgcctacg gcgagcgcgt ttccgtcacc tcgtttttcg acctggtgct gttctataac
      781 gagggcgccg cgttcagttt tttggccgcc gccgggggct ggcagcgcct gtttttcagc
      841 gccttcgccg tcatcgcttc cgtggtcatt atccatttac tgcgcaagca tcccgggcag
      901 aagctgttct gccttgccct cgctctgatc ctgggcgggg cgctgggcaa cctctacgac
      961 cgcctcacac tgggctacgt ggtggatttc ctctatttcc attaccaggc ctggtactgg
     1021 ccggccttca acgtggccga ttcggcgatt tcggttgggg tcgtcctgct gttgtgggac
     1081 ggcttgaaaa agccgtaagc caggcgcacc atctccgaca aagcagcatc aaattacgag
     1141 gagactgcca tgccggactg gaaaaagctg atcgaaggca agatcgcact ggccaaacgc
     1201 ggccttaagc cgggcgcttc cagcagccat gcgcgtgttc ccgccgggca aaccctggtg
     1261 actaattttc ctgtgctgca catcagcgaa ccgcccaggc tcacctctgc cgactggaag
     1321 ttgcgcgtgt atggcctggt cgagaatgcg ctctcgctgg actggcaggc gttccgacaa
     1381 ctgccccaga tcaaggatgt ttcggatttc cactgcgtca ctcgctggtc caagctggac
     1441 gtggaatggg agggcgtgcg tgccagcgag gtgctggcgc tggccatgcc gctggacact
     1501 gcgaaatacg tcaccttaca cagctacgat ggctacacta ccaatttgcc cctggaagcg
     1561 ctgctggacg aggacgtcat cgtcgcccac agcgtcctcg gccagccgct ctccgccgac
     1621 cacggcggtc cggtgcggct ggtggtgccc aaacgctatg cctggaaaag cgccaaatgg
     1681 ctgaaggcca tcgagctgca tgccgaggac cggccggggt tctgggagga gcgcggctac
     1741 cacaacgagg cagatccgtg gaaggaagag cggttcagcg ggaattggta gaaatgcgac
     1801 ctgacatgcc aaaaaccttg gccttccaag gcgagactac ccgggcaaga gcagccaatt
     1861 aaccagcgca gataaaaaag ccggcattcg ccggcttttt tgttcctgtt gtatgagagt
     1921 caattcattt tccacttgat gctcaccgct ccccgaattg aacctgcgct atagccggtg
     1981 gcctggtcgt cgggataatc cttggccagc agcgccttca cgccatccga aatctgataa
     2041 ggctggccgt ggcattgcag gcacatgggc tgagtgggga tagccttcat gtagcggaac
     2101 cagcggccgt caggagtttc caccaccgtg tttttctcca tggaggcaat gggcttgccg
     2161 ccggaaagtt cgttgtcgaa gctttccagc atctgttttt cccaagtgtc cggagtgccg
     2221 agtgccggat tgcgcggctt gagggaaacg cgcttgaccg cgcggttgtc gccggaatac
     2281 tccgctgcca gtgctggcgc atattgtttg cagacgccaa tggcgttttc cgcgcctccg
     2341 acctcgattt gctgcttgag catcgtgccc agcttgagca tgaactcctg ggcggtctcc
     2401 cggctgtcct tgagatattt ttcctgcaga tcgtcggcca gcgcgggtcc tgatacagcg
     2461 agcgccagca gggcggaaat gagtgggttt ttcacgataa tcggctcctt gagggatgcc
     2521 caaaaataac gcgttccacg gtgcgaatca agagacttat tttttcgggt tcttgcccag
     2581 tttccgttgc agcgtgcggc ggtgcatgtc gagcgcgcgc gccgtggcgg aaatgttgcc
     2641 gtcgtgctcg gccagtatgc gctggatgtg ttcccattcc agcctttcca ccgatagcgg
     2701 agaggtattg agcggggcgg ctgcatcgcc ctccacgcgg tagaaggcgg caacgatttc
     2761 gtctgcatcg gcaggcttgg ccaggtagtg cgttgcgccc agcttgaccg cttccaccgc
     2821 ggtggcgatg ctggcgaaac cggtaagcat gacgatgcgg gtggcggcgt cgagttcctt
     2881 caggcgcttg accagcacca gcccggactg gccgggcatc ttgaggtcga ccacggcata
     2941 ttccggcggg ttggcctcgg ccagcgccag tgcctgggcc acatcggctg cagtgctgac
     3001 atcgaaaccg cgctttttca aggcgggcgc tagcacctgg cggaaggtgt cgtcgtcgtc
     3061 caccagcagc agggaagggc attcttccag tgcgtggctc atggttttcc tttaagtagc
     3121 aggccgttga gcggcaagct gacgcgggtc agcgtgccac cttgcgggtg gttttcacgg
     3181 ctgacgctgc caccgaagcg tacgtaaatc agacgcgcaa gctagaggcc gaatcacag
//
LOCUS       DAOMFA010000166         3237 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_4215_length_3237_cov_5.083281, whole genome shotgun sequence.
ACCESSION   DAOMFA010000166 DAOMFA010000000
VERSION     DAOMFA010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 3237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3237
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4215_length_3237_cov_5.083281"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(253..807)
                     /locus_tag="PKW44_07735"
     CDS             complement(253..807)
                     /locus_tag="PKW44_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018076845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HQR51512.1"
                     /translation="MVSLQTPICDFGWKAPDFSLQGVDGNIWRLADIRGPKGTLVMFI
                     CNHCPYVKAIRPRLVSDLRELKALGINAIAIMSNDPTDYPEDGFDHMREVAREFDFSF
                     PYVIDPTQQVARAYGAICTPDFFGFNANLELQYRGRFDESRKETAPDSTRDLFHAMKQ
                     VAETCQGPREQISSIGCSIKWIAE"
     gene            873..1604
                     /locus_tag="PKW44_07740"
     CDS             873..1604
                     /locus_tag="PKW44_07740"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HQR51513.1"
                     /translation="MSSPRFFCDAKLGPGALLQLPENAARHAARVLRLKVGDRVTLFN
                     GDGLNYSAELLRVERDDVSVKITEFVTVERESPLQVTLAQAISSGDRMDFTLQKSVEL
                     GVTAIQPLATERSVVKLSGERADKRRQHWQNVVVSACEQSGRAIVPPVAMPTPLINWL
                     GEPETFVLKLMLAPGAEHTLRDLPKPAGDICLLIGCEGGFAPQEVVAAESRDFIPVRL
                     GARVLRTETAAMAALAAMQTLWGDF"
     gene            1606..>3237
                     /locus_tag="PKW44_07745"
     CDS             1606..>3237
                     /locus_tag="PKW44_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HQR51514.1"
                     /translation="MIAILEAKRAGLLAKTHWANNLLAGVIVGIVALPLAMAFAIASG
                     AKPEQGLYTAIVAGALTSLFGGSRLQIAGPTGAFIVILSGITAKYGIEGLQVATLMAG
                     VMLLLMGFAKLGGAIKYIPDPVIVGFTSGIAVIIWVGQWKDFFGLSIFGSEDHFHEKL
                     WQLVQAFPTLNLETPLLALFTLAALLLSPRVFKRIPSPLVAMVAATVLQGFFQFNGVA
                     TIGSAFGGIPQSLPKFEPPSLTFGEMLHLIGPAFTIALLGAIESLLSAVVADSMSGSG
                     KRHDSNQELIGQGIANIFSPLFGGFASTGAIARTATNIRNGGTSPLAGIIHALTLVLI
                     VLLLAPLAAHIPLCALSAILFVVAYNMSEWRRFIHMARTAPKADVAVLLITFLLTVFS
                     DLVIAVNIGVLLAALLFMKRMSEAVSVEQQTHDDLREEVGDPGFSLPPGTVVYAMEGP
                     FFFGAAERLESTLESIHGHASILVLRMEQVPFVDATGLQTLWDLLDNCERHKTRLALC
                     GARPNVLEKLQRAGLAGRIGEKNILQFIWELGQGKP"
BASE COUNT          612 a         1037 c          944 g          644 t
ORIGIN      
        1 caccatccac cgacagtgca tggatggcat ttgccagttc tttacgagtc accataatca
       61 ttcctatcgt taagcttaaa gagaaaaaga ctcctacctc ttcaggcaga agccatacct
      121 caaaatcatt tcggaattcc ccatggagtc aggcaattat atttttatgg ttgcccgcgg
      181 cgggagaatg gctcaattat tcccgaattg cgcgctctgg gcaatagccg caaggccgta
      241 ttaacgataa ggttactctg caatccactt gatggaacag ccgatggacg aaatctgctc
      301 gcgcggccct tggcaggttt ccgcgacctg cttcatggcg tggaacaggt cgcgcgtgct
      361 gtccggcgcg gtttccttgc gcgattcgtc gaacctgccg cggtactgca attccagatt
      421 ggcattgaag ccgaagaaat cgggggtaca gatcgcacca taggctctgg cgacctgctg
      481 ggtcggatcg atcacgtagg ggaacgaaaa atcaaactcg cgcgccacct cccgcatgtg
      541 atcgaagccg tcttccgggt aatcggtcgg gtcgttggac atgatggcga tggcgttgat
      601 gcccagcgcc ttcaactcgc gcaagtcgct gaccaggcgc gggcggatgg ctttgacgta
      661 agggcagtgg ttgcagatga acatcaccag cgttcccttg gggccccgta tgtcagccaa
      721 gcgccagata ttgccgtcca cgccctgcag gctgaaatca ggcgccttcc agccaaaatc
      781 acagatcggg gtttgcaggc ttaccatgag aagactcctt ttcctttaca attcaggtca
      841 gattttacca cccactgctg acggccaacc tcatgtcctc accacgcttt ttctgcgatg
      901 ccaaactcgg ccccggcgcc ctgctacagc tgccggagaa tgccgcgcgc cacgccgccc
      961 gtgtgctgcg cctgaaggtt ggcgaccggg ttaccctgtt caacggcgat ggtctcaact
     1021 acagcgccga actactacgc gtcgagcgcg acgacgtcag cgtcaagatt accgagttcg
     1081 tcacggtgga acgcgaatcg ccgctgcagg tgacgctggc gcaggccatt tcctccggcg
     1141 accgcatgga tttcaccctg cagaaatcgg tggaactggg ggtgaccgcc atccagccgc
     1201 tcgccaccga gcgcagcgtg gtcaagctca gcggcgagcg cgccgacaag cgccgccagc
     1261 actggcagaa cgtggtggtt tccgcctgcg agcagagcgg ccgcgccatc gtcccgccgg
     1321 tcgccatgcc tacgccgctc atcaactggc tgggcgagcc ggaaaccttt gtgctgaaac
     1381 tgatgctggc tcccggtgcc gaacacacgc tgcgcgacct gcccaagccc gccggcgaca
     1441 tctgcctgct gatcggctgc gaaggcggct ttgccccgca ggaagttgtg gcggcggagt
     1501 cgcgcgactt catcccggtg cggctgggtg cgcgcgtgct gcgtactgaa accgccgcca
     1561 tggcggcctt ggcagccatg cagacgttgt ggggagattt ctaacatgat cgccatcctc
     1621 gaagccaagc gcgccggtct gcttgccaaa acgcactggg ccaacaacct gctggcaggc
     1681 gtgattgtcg gcatcgtagc actcccgctg gccatggctt tcgccatcgc ctccggcgcc
     1741 aagccggaac aagggcttta cactgccatc gtcgccggcg ccctcacttc gctgttcggt
     1801 ggcagccgcc tgcagatcgc cggccccacc ggcgccttca tcgtcatcct gtcaggtatc
     1861 actgccaagt acggcatcga aggcttgcag gtggcgacac tgatggccgg cgtcatgcta
     1921 ctgctgatgg gattcgccaa actgggcggt gctatcaagt acatccccga cccggtcatc
     1981 gtcggcttca ccagcggcat cgcggtcatt atctgggtcg gccaatggaa ggattttttc
     2041 ggcctgagta tttttggcag cgaagaccat ttccatgaaa aactgtggca actggtccaa
     2101 gccttcccca ccctgaacct tgaaaccccg ctgctggcac tattcacgtt ggcggcactg
     2161 ctgctgtcgc cgcgcgtttt caagcgaatc ccgtcgccgc tggtggcgat ggtcgcagcc
     2221 accgtcctgc aaggattttt ccagttcaac ggcgtggcca ccatcggtag cgccttcggc
     2281 ggcatcccgc aaagcctgcc caagttcgaa ccgccctcgc tgaccttcgg cgaaatgctg
     2341 cacctgatcg gcccggcctt caccatcgcc ctgctcggtg ccatcgaatc gctgctctcc
     2401 gccgtggtgg cggacagcat gagcggcagc gggaagcgac acgattccaa tcaggagttg
     2461 atcggccagg gcatcgccaa cattttctcg cccctgttcg gcggctttgc ttccaccggc
     2521 gccatcgccc gcaccgccac caatatccgc aatgggggca ccagcccgct agccggaatt
     2581 atccatgcgc tgaccctggt gctgatcgta ctgctgctgg cgccgctggc cgcccacatc
     2641 ccgctgtgcg cgctatcggc catcctgttc gtggtggcct acaacatgag tgagtggcgt
     2701 cgtttcatcc acatggcgcg tacggcgccc aaagcggatg tcgcggtgct gctcatcact
     2761 ttcctgctta ccgtgttcag cgacctggtc atcgccgtga acatcggcgt gctgctggcg
     2821 gccctgctgt tcatgaaacg catgagcgaa gccgtcagcg tcgaacagca gacgcacgac
     2881 gacctgcggg aagaagtggg cgacccgggc ttttcgctac cccccggcac cgtggtgtac
     2941 gccatggaag gaccgttttt cttcggcgcc gccgaacgtc tggaaagcac gctggaaagc
     3001 attcacgggc atgccagcat cctggtgctg cgcatggaac aggtgccgtt cgtagacgcc
     3061 accggcctgc aaaccctgtg ggatctgctg gacaactgcg agcgccacaa gactcgcctg
     3121 gcgctatgcg gcgcgcgccc caatgtgctg gaaaaactgc agcgcgccgg gctggccgga
     3181 cggataggcg agaaaaatat actgcagttc atctgggagc ttgggcaagg caagccg
//
LOCUS       DAOMFA010000167         3192 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_4309_length_3192_cov_5.254702, whole genome shotgun sequence.
ACCESSION   DAOMFA010000167 DAOMFA010000000
VERSION     DAOMFA010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 3192)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3192)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3192
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4309_length_3192_cov_5.254702"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..184)
                     /locus_tag="PKW44_07750"
     CDS             complement(<1..184)
                     /locus_tag="PKW44_07750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR51515.1"
                     /translation="MKRLIRTACAAMPGTNFSAHLLKQLGSASGRRCIFLVNILDIIE
                     KYIKNNNTIKKTKLNPL"
     gene            complement(225..>3192)
                     /locus_tag="PKW44_07755"
     CDS             complement(225..>3192)
                     /locus_tag="PKW44_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HQR51516.1"
                     /translation="QVVIQTEAPGLSADLVETLVSQPIENAISGTVGIESMRSQSIPG
                     LSVVTIIFTEGTDVYRNRQVVAERLSTLTSQLPRNVIPNITPLASSASTVLGIGLTSD
                     ARSLMELRTLVDWSIRPHLMAVPGVADVNVFGGEVRQFQIQVDPEKLIRYNLSLQEVL
                     AAAAKATGVRGAGFIEGSNQRILINSEGQALTPVQLARMPMLHRDGQTIRMGDIGQVV
                     EGPAPSISAAAINGKPGVYLSVQGQLGANTKAVTDALEKALHELEPSLQNEQVKLYAE
                     LFRPANFIETAIDGVKTDILIGSVLVIAVLFLFLFNVRTAFISATAIPLSLLTAVIVL
                     AYFGIGLNIMVLGGLAIALGEVVDDAIIDTENIFRRLRENRLLAEPRPAHLVVFDASM
                     EVRSSVVYATIIVAMVFMPLLTLSGVAGKLFAPLGYAYISAILASLVVALTLTPALCY
                     LLFTHGKLESEDPPLIRLIKRGYTGLLRRIERHYRATLSLSMVLILLGLGLLPLFKSQ
                     FIPNLHEGHYILHMTAVPGTSEQESLRIGEKVAQAVSRIKGIQSVAQWVGRAQNGADT
                     FGTHYSEFEVELGKLSGEEQNHILREIRETLAGKNGESFPGVNFAINTFLTERIEETI
                     SGYTASVVINIHGQDLDALDHDARSVAAVLGSIPGATDVQIQSPPGTPQLTIRLRPER
                     LAQWGMAPLDVLENIQAAFEGVQAAQVYQGNHVIGVNVMFTPQLRDDLREIGDLPLKN
                     PDGKIIRLRELADIAQENGRSKILHAGAKRIQTITCNVANRDIESFAREMKKRIPAEA
                     RLSPGNFATFTGEAEANAQAREDLVVHSLLAGVGIFLMLYIAFGRLRNLLLAFVNLPF
                     ALIGGVLAALFTGGWISLGSLVGFVTLFGITLRNSIMLVSHYQHLVDEEGMTWGLETA
                     IRGASERLPSILMTALVTALGLLPLAAGSGQPGREIEGPMATIIVGGLVTSTILNLLI
                     LPSIMLHFGRFEKRQF"
BASE COUNT          573 a          900 c         1090 g          629 t
ORIGIN      
        1 ataagggatt tagttttgtt ttttttattg tattattgtt ttttatatat ttttcaataa
       61 tatccaaaat attgaccaag aaaatacacc ttctgccact ggcgctccca agctgcttga
      121 gcaggtgcgc ggaaaaattc gttcctggca ttgcagcaca cgcagtccgg attaaacgct
      181 tcatcctgca ttctgacaag cattgccctt gggcttaccc tggcctagaa ctgtcttttc
      241 tcgaagcgcc cgaaatgcag catgatggag ggcaggatca gcaggttgag gatggtggag
      301 gtgaccaggc cgccgacgat gatggtggcc atggggcctt cgatttcgcg ccccggctgg
      361 ccgctgccgg cggccagtgg cagcaggccg agcgcggtga ccagcgccgt catcaggatc
      421 gagggcaggc gctcggaggc gccgcgaatg gcggtttcca ggccccaggt catgccttcc
      481 tcgtcgacca ggtgctggta gtgcgacacc agcatgatgg agttgcgcag ggtgatgcca
      541 aacagggtga cgaagccgac cagcgagccg agcgaaatcc agccgccggt gaagagagcg
      601 gccagtacgc cgccgatgag ggcgaacggc aggttgacga aggcaagcag caggttgcgc
      661 aggcggccga aggcgatgta gagcatgagg aagatgccga cgccggccag cagcgagtgc
      721 acgaccaggt cctcgcgcgc ctgggcgttg gcctcggcct cgccggtgaa ggtggcgaag
      781 ttgccggggg agaggcgtgc ctcggccggt atgcgctttt tcatctcgcg ggcaaagctc
      841 tcgatgtcgc ggttggccac attgcaggta atggtctgga tgcgcttggc gccggcgtgc
      901 aggatcttgg aacggccgtt ttcctgagca atgtcggcca gttctcgcag gcggatgatc
      961 ttgccgtccg ggttctttag cggcaggtcg ccgatttcgc gcaggtcgtc gcgcagttgc
     1021 ggggtgaaca tgacgttgac gccgatgacg tggttgccct ggtaaacctg tgctgcctgt
     1081 accccttcga atgccgcctg gatgttttcc agcacgtcca gcggcgccat gccccattgc
     1141 gccagccgtt ccggacgcag gcggatggtc agttgcggcg tgcctggggg cgactggatc
     1201 tgcacgtcgg tcgcgccggg aatgctgccc agcacggcgg ccacgctgcg tgcatcgtgg
     1261 tccagcgcat ccaggtcctg gccgtggatg ttgatgacta cggaggcggt atagccggag
     1321 atggtctcct cgatgcgttc ggtgaggaag gtgttgatgg cgaagttgac gccggggaag
     1381 ctttcgccgt tcttgccggc cagggtctcg cgtatttcgc gcaggatgtg gttctgttcc
     1441 tcgccggaga gtttgccgag ctcgacctcg aattcgctgt aatgcgtgcc gaaggtgtcg
     1501 gcgccgttct gcgcgcggcc tacccactgc gctacggact ggatgccctt gatccggctc
     1561 accgcctgcg ccaccttttc cccgatgcgc aggctttcct gttccgaagt gccgggcacg
     1621 gcggtcatgt gcaggatgta atgtccttcg tgcaggttgg gaatgaactg gcttttgaac
     1681 aaaggaagga ggcccaggcc gagcaggatg agcaccatgg acaggctgag cgtggcgcgg
     1741 taatgccgct cgatgcgccg cagcaggccg gtgtagccgc gcttgatgag gcggatcagc
     1801 ggcggatcct cgctctccag cttgccgtgg gtaaacagca ggtagcacag cgccggggtc
     1861 aacgtcagcg ccaccaccag cgaggccaga atggcgctga tataggcgta gccgagcggt
     1921 gcgaacaatt tgccggccac gccggacaag gtgagcaggg gcatgaacac catggcgacg
     1981 atgatggtgg cgtagaccac cgagctgcgc acttccatgg aggcgtcgaa cacgaccagg
     2041 tgtgccggcc gtggctcggc cagcaggcgg ttttcgcgca ggcggcggaa gatgttttcg
     2101 gtgtcgatga tggcgtcgtc tactacttcg cccagcgcga tggcgagccc gcccaacacc
     2161 atgatgttga ggccgatgcc gaaatacgcc agcacgatga ccgcggtcag cagcgagagc
     2221 gggatggcgg tggccgagat aaaggcggtg cgcacgttga acaggaacag gaacagcacc
     2281 gcgatcacca gcaccgagcc gatcaggatg tcggtcttga cgccgtcgat ggcggtttcg
     2341 atgaagttgg ccgggcggaa cagctcggca tagagcttca cctgttcgtt ttgcagcgaa
     2401 ggttccaatt cgtgcagggc tttttccagt gcgtcggtta cggccttggt gttggcgccc
     2461 agttgcccct gcacggagag gtagacgcca ggtttgccgt tgatggccgc ggcgctgatg
     2521 gaaggggcag ggccctcgac cacctggccg atgtcgccca tacggatggt ttgcccatcg
     2581 cggtgcagca tcggcatgcg cgccagttgc accggggtga gcgcctggcc ttcgctgttg
     2641 atgaggatgc gctggttgct accctcgata aagccggcgc cgcgtacccc ggtggctttg
     2701 gctgcggccg ccagtacctc ctgcagcgac aggttgtagc ggatcagctt ttccgggtcg
     2761 acctggatct ggaactggcg tacctcgccg ccgaacacgt tgacatcggc cacgccgggc
     2821 accgccatca ggtgtgggcg gatgctccag tccaccaggg tgcgcagctc cattagcgaa
     2881 cgggcgtcag aagtcaggcc gatgcccaat accgtgctgg cggaggaggc cagcggcgtg
     2941 atgttgggga tgacattgcg cggcaactga ctggtcagcg tggaaaggcg ttccgccacc
     3001 acctgccggt tacggtaaac gtcggtgcct tccgtgaaaa tgatggtgac caccgatagt
     3061 ccggggatgg actgggagcg catggactcg atgcccaccg tgccggagat ggcgttttcg
     3121 ataggctgcg aaaccagcgt ttccaccaga tcggcggaaa gtccgggcgc ctcggtctgg
     3181 attaccactt gg
//
LOCUS       DAOMFA010000168         3085 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_4529_length_3085_cov_3.580858, whole genome shotgun sequence.
ACCESSION   DAOMFA010000168 DAOMFA010000000
VERSION     DAOMFA010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 3085)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3085)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3085
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4529_length_3085_cov_3.580858"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..324
                     /locus_tag="PKW44_07760"
     CDS             <1..324
                     /locus_tag="PKW44_07760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR51517.1"
                     /translation="ASNADAASQDDQTVAPSAEAKSGAKGKTAASKKQSADAEAKRKE
                     QENLAIKKTRDENCANARNELINYKIGGRMYKVDENGERVFIDDQQIESGKAAAQKEV
                     DRWCD"
     gene            complement(371..688)
                     /locus_tag="PKW44_07765"
     CDS             complement(371..688)
                     /locus_tag="PKW44_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019917544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HQR51518.1"
                     /translation="MNNEMLVEVAYALPQQQLILPVRVSAGTTAEQAVQASGILQKFP
                     GIDLSRSQLGIFGKLIKSDTVLREHDRVEIYRELIADPKEVRRQRAAEGKAMKKGGGA
                     LEA"
     gene            complement(681..1112)
                     /locus_tag="PKW44_07770"
     CDS             complement(681..1112)
                     /locus_tag="PKW44_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HQR51519.1"
                     /translation="MAQVEKSVLVPHSAERMFRLVDEAERYPEFLPWCGGTDLKWRDK
                     ATTVATLHINYHGVRQHFTTENSKSFPTLMDIRLVDGPFRHLEGVWRFTPLAEDACKI
                     EFKLHYEFSSHLLEKIIAPVFSHIANTFVDAFVERAEKIYE"
     gene            1165..1614
                     /gene="smpB"
                     /locus_tag="PKW44_07775"
     CDS             1165..1614
                     /gene="smpB"
                     /locus_tag="PKW44_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HQR51520.1"
                     /translation="MSIAQNKKAFHEYFIEEKYEAGVVLEGWEVKAIREGKVQLKEAY
                     VIIQHGEIYIIGCHITPLGSASTHVRPDATRTRKLLLHSEQIAKLVGKVERAGYTLVP
                     LDMHYTRGRIKIEIGLAKGKKQYDKRESEKERDWEREKGRIMRARNK"
     gene            1675..2037
                     /gene="ssrA"
                     /locus_tag="PKW44_07780"
     tmRNA           1675..2037
                     /gene="ssrA"
                     /locus_tag="PKW44_07780"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(2305..2640)
                     /locus_tag="PKW44_07785"
     CDS             complement(2305..2640)
                     /locus_tag="PKW44_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005673685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HQR51521.1"
                     /translation="MELLKVAILFAVTAVAEIVGCYLPWLVLKQDKPAWLLIPAALSL
                     GLFAWLLTLHPTAAGRTYAAYGGMYIAVALVWLHFVDGVALTRWDVAGASIALAGMAV
                     IALQPATGA"
     gene            complement(2643..3050)
                     /gene="merR"
                     /locus_tag="PKW44_07790"
     CDS             complement(2643..3050)
                     /gene="merR"
                     /locus_tag="PKW44_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001166627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001228 - DNA-binding transcription
                     activator activity, RNA polymerase II-specific [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hg(II)-responsive transcriptional regulator"
                     /protein_id="HQR51522.1"
                     /translation="MNELPETLTIGVLADAAGVNVETIRFYQRKGLMQEPDRPLGGIR
                     RYGEPDLARVRFIKSAQRLGFSLDEVGDLLKLEDGSHCTEARAQAERKLADVRDRLAD
                     LRRIEVVLQDLVQRCCAAHGEVRCPMIQALQEA"
BASE COUNT          675 a          908 c          915 g          587 t
ORIGIN      
        1 gccagcaacg ctgacgccgc atctcaggac gatcagaccg tggcgccctc tgccgaggcc
       61 aagtccgggg cgaaggggaa aacggctgcc agcaaaaaac agtcggctga tgccgaggcc
      121 aagagaaaag agcaggaaaa tcttgccatc aagaaaaccc gcgatgaaaa ctgtgctaat
      181 gccagaaacg aactgatcaa ctacaagatc ggcgggcgca tgtacaaggt ggatgagaat
      241 ggcgaacggg tattcatcga cgaccagcaa atcgagtcag gcaaagcggc ggcacaaaaa
      301 gaggtcgacc ggtggtgcga ctagctaacg gagacgggct ggattcattg gcggtttagg
      361 ccaaatccgg tcaggcttca agcgctccgc cgcctttctt catggccttg ccctcggccg
      421 ctctctggcg gcgcacttcc ttggggtcgg cgatcagttc ccggtagatt tccacgcggt
      481 cgtgttcgcg caagaccgtg tccgacttga tcagcttgcc gaaaatgcca agctggctcc
      541 gggacagatc aattcccgga aacttctgca gaatcccgct ggcctgcacc gcctgctcgg
      601 cagtggtgcc ggcgcttacc ctgaccggca ggatcagctg ttgctgcggc agggcgtagg
      661 cgacttcgac aagcatctcg ttattcataa atcttttccg cgcgctcgac gaatgcgtcg
      721 acgaaggtat tggcgatatg gctgaacacc ggggcgatga ttttctccag taaatggctg
      781 gagaactcgt agtgtaactt gaattcgatt ttgcaggcgt cctcagccag aggggtgaag
      841 cgccagacac cttccaggtg tcggaatggg ccgtctacca gacggatatc catcagggtc
      901 gggaaactct tgctgttctc ggtggtgaaa tgctgcctaa ccccgtgata attgatgtgc
      961 agcgtggcga ccgtggtagc tttgtcgcgc catttcaggt ccgtgccacc acaccagggc
     1021 aggaattccg gatagcgctc tgcctcgtcc accagacgga acatgcgctc ggcggagtgg
     1081 ggaaccagaa cggatttttc gacttgcgcc atgaggctgg gctgtatgct gtaaaattac
     1141 gccattttaa gccaaaccca taccatgagc atcgcccaga acaagaaagc ctttcacgaa
     1201 tacttcatcg aagagaagta cgaggcaggt gttgtgctgg aaggctggga ggtcaaagcc
     1261 atccgcgaag gcaaggtgca gctcaaggaa gcctacgtca ttatccagca tggcgaaatc
     1321 tacatcatcg gctgccacat cacgccactg ggctcggctt ccacccatgt aaggcccgat
     1381 gccacccgta cccgcaaact actgctgcac agcgagcaga tcgccaagct ggtcggcaag
     1441 gtcgagcgcg ccggttatac cctggtgccg ctggacatgc actacacccg tggccgcatc
     1501 aagatcgaga tcggcctggc caagggcaag aagcagtacg acaagcgcga atccgagaag
     1561 gagcgcgact gggagcgcga gaagggcagg atcatgcggg cgcgcaacaa ataacccgcg
     1621 tataatgccg catctggtga acttctctgg ggaacgccag tctgtagtac ctctgggggc
     1681 gacctggctt cgacgtgggt tgcaaagcag agcagggcat accgaggacc cgccacctcg
     1741 taaataagct ggaaaaagta tagtcgcaaa cgacgaaact tacgctctag ccgcttaatc
     1801 ccggctggac gctgcaccga agggcctctc ggtcgggcag ggcaacctgc agcagcgtca
     1861 tatagagagg ctcgtgtcgt gcagggtcac tttgcacgat attaaatcca aggtgactcg
     1921 ccttgcctca gctcgccggt tggcggttgc agggttaaat caaacagcca ggctaagtat
     1981 gtagaactgt ctgtagagtg cttgcggacg ggggttcgat cccccccgcc tccaccaaaa
     2041 cccaaaatcc caacctttat cggttgggat tttttttgcc cgaaacgcca gtgttggcgc
     2101 ggtttctggc cttggcctct tgagcgcgac cctccggaag tctccgttcc aggcgcgctt
     2161 tccgccgttt ttctctctct gttctctgct gtcctcttga ccgtgcaagt gccgaagtcc
     2221 gctggtttcg cgggtttgag ggtcagcagt tgcgtttttc gtctgcatgg cgcaggcgac
     2281 cgagaacgga gaaaggcggg ggcgtcacgc gcccgtcgcc ggttgcagcg cgatgaccgc
     2341 cataccggcc aaggcgatgc tggctcccgc aacgtcccag cgggtcaggg ccacgccatc
     2401 gacgaagtgc agccagacca gcgctacggc gatatacatc cctccgtagg ccgcgtaggt
     2461 tcgccccgcc gcagtcggat gcagcgtcaa cagccacgca aacagcccga gcgacaaggc
     2521 agccggtatc agcagccacg cgggcttgtc ctgcttgagg acaagccagg gcaagtagca
     2581 gccgacgatt tcagccaccg ctgtcacggc aaacaggatg gcgactttca acagttccat
     2641 cgtcatgcct cctgcagagc ctggatcatc ggacagcgca cttcgccgtg ggcggcacag
     2701 cagcgctgca ccagatcctg cagcacgact tcgatgcggc gcaggtcggc caagcggtcg
     2761 cgcacatcgg ccagtttgcg ctcggcctgc gcgcgggcct cggtgcaatg cgatccatcc
     2821 tcaagcttca gcaaatcccc gacctcgtcc aggctgaatc ccaggcgctg ggccgatttg
     2881 ataaagcgca cgcgcgccag atccggctca ccgtagcgac ggatgccacc caaaggccgg
     2941 tcgggctcct gcatcaagcc tttgcgttga tagaagcgga tcgtctcgac attgaccccg
     3001 gcggcatcgg ccaggacgcc gatggtcagt gtttcgggta gttcattcat ggcgcttgac
     3061 tccgtactta agtacggaag taagc
//
LOCUS       DAOMFA010000169         3056 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_4583_length_3056_cov_4.439853, whole genome shotgun sequence.
ACCESSION   DAOMFA010000169 DAOMFA010000000
VERSION     DAOMFA010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 3056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3056
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4583_length_3056_cov_4.439853"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..520)
                     /locus_tag="PKW44_07795"
     CDS             complement(<1..520)
                     /locus_tag="PKW44_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008059095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HQR51523.1"
                     /translation="MARLEDGQLVFHDSLREMVRLGAGLGKDKYLDAISQQRAIDCLK
                     RFSERLRGFDPHAVRAVATNTFRVARNAPQLLEAAQQALGFPIEVIAGREEARMIFIG
                     VSHGLPPAEGKRMVMDIGGGSTEFIVGEGFEPQLMESLYMGCISYSMRFLGDDKLSER
                     NLKKAEISARVEN"
     gene            complement(618..1319)
                     /gene="phoU"
                     /locus_tag="PKW44_07800"
     CDS             complement(618..1319)
                     /gene="phoU"
                     /locus_tag="PKW44_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HQR51524.1"
                     /translation="MSEHTYKQYDTELESVRAKVLQMGGLVEQQIVDALQALVHADPK
                     LADEVIANDHRVNALEVSIDEDCSHIIARRQPAASDLRMIMMVVKTITDLERIGDEAT
                     KIARVALKIHDSGRMYSPRFSEIKNMVGQVCEMLRISLDAFARLDVTRTVEVARQDEQ
                     VDEHFRSAMRQLITFMLEDPRTISMTLEVLFVAKAVERIGDHAKNIAEYVVYMVKGKD
                     VRHTSVEEMERETQQ"
     gene            complement(1386..2048)
                     /gene="rpiA"
                     /locus_tag="PKW44_07805"
     CDS             complement(1386..2048)
                     /gene="rpiA"
                     /locus_tag="PKW44_07805"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012777654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HQR51525.1"
                     /translation="MATQDELKQQVARAAAEYVRGGIIGVGTGSTANYFIEELAKVKH
                     KIDGAVASSESTAQRLRGHGIEVLDLNSVNVGDMGIYVDGADEITEHFHMLKGGGGAL
                     TREKIVAAVAGKFICICDASKLVPVLGKFPLPVEVLPMARSQVARELVKLGGQPQLRD
                     FTTDNGNVILDVYGLTILDPVALEAKINQIVGVVTNGLFAARPADVLLLATQDGVKTY
                     TR"
     gene            2125..>3056
                     /gene="ilvA"
                     /locus_tag="PKW44_07810"
     CDS             2125..>3056
                     /gene="ilvA"
                     /locus_tag="PKW44_07810"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013440989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HQR51526.1"
                     /translation="MKNDYLEMILTARVYDVAVETLLEAQPNLSRRIGNTVLLKREDM
                     QPVFSFKLRGAYNKMAHLTAAQLKRGVIAASAGNHAQGVALAAQKLGCRAVIVMPTTT
                     PQIKVEAVRNRGAEVVLAGDSYSDAYTHALALEKKEKLTFVHPYDDPDVIAGQGTIAM
                     EILRDHPDPIHAIFCCVGGGGLIAGIAAYVKRLRPEIRVIGVEASDADAMSQSLVKGH
                     RVRLNQVGLFADGAAVKIVGEETFRLCREYVDEMVQVDTDAICAAIKDVFEDTRSILE
                     PAGALAIAGAKAWAARHKLRNETLVAVASGANTN"
BASE COUNT          634 a          955 c          845 g          622 t
ORIGIN      
        1 agttttcgac acgggcggaa atctcggctt ttttcaggtt acgttcgcta agcttgtcat
       61 cgcccaaaaa acgcatgcta taactgatgc agcccatgta caggctttcc atcagctgcg
      121 gttcgaaacc ctcgccgaca atgaactcgg tggacccgcc gccgatgtcc atcaccatgc
      181 gtttaccttc ggcagggggc agtccatggc tgacgccgat gaaaatcatc cgtgcctcct
      241 cgcgccctgc gatgacttcg atagggaacc ccagcgcctg ttgtgccgct tccagcaatt
      301 gcggggcgtt tctggcgaca cggaaagtat tggtggcaac ggcacgcacg gcatgcggat
      361 cgaaaccgcg caggcgctcg ctaaatcgtt tcaggcagtc gatggcacgc tgctgggaga
      421 tagcatcgag atatttgtcc ttgcctagtc cggcgcccag ccgcaccatc tcgcgcaggg
      481 agtcatgaaa gaccagttgc ccgtcctcca gccgcgccat ttgcaggcga aaactgttgg
      541 aacccaagtc aacggcggcg atgtcggaca agccagctac acgggcactc acgattccgc
      601 acctccgtca gccaaggtca ttgctgggtt tcgcgctcca tctcctccac cgaggtatgg
      661 cgcacatcct tgcccttgac catgtacacc acgtattcgg cgatgttctt ggcatggtcg
      721 ccgatgcgct cgacagcctt ggccacgaac agtacttcca aagtcatgga aatagtgcgt
      781 ggatcttcca gcatgaaggt gatgagttgg cgcatggcgg aacgaaaatg ttcgtccacc
      841 tgctcgtcct ggcgcgccac ctccactgtc cgggtaacat ccagacgggc aaaagcatcc
      901 agcgatatgc gcagcatttc gcatacctgg cccaccatgt tcttgatctc gctgaaacgt
      961 ggagaataca tgcggccaga atcgtgaatc ttgagggcaa cgcgggcaat tttggtggct
     1021 tcgtcgccga tgcgctcgag atcggtgatg gtcttaacca ccatcatgat catgcgcaaa
     1081 tcactcgccg ccggctggcg gcgcgcgatg atatggctgc aatcctcgtc gatcgagact
     1141 tccaaggcat tgacacggtg gtcattggcg atgacctcgt ccgccaactt gggatcggca
     1201 tgcaccagag cctgcagcgc atccacgatc tgctgctcga ccagaccacc catctgcagc
     1261 accttggcgc gcaccgattc gagctcggta tcatattgtt tgtaggtatg ttcagacatg
     1321 gaacgatcct ccgggtaata gacccgacag catagcgtgg aaatatgaca gtaacgttac
     1381 cttaatcagc gggtataggt cttgacgcca tcctgcgtcg ccagcagcag cacgtcagcc
     1441 ggacgcgcgg caaacaggcc gttggtcacc acgccgacga tctggttgat tttcgcttcc
     1501 agcgccaccg gatcgaggat ggtcaggcca tacacatcca gaatcacgtt gccgttatcc
     1561 gtcgtgaagt cacgcaactg cggctgaccg ccgagcttga ccagctcgcg cgccacctgg
     1621 ctgcgcgcca tcggcagcac ttccaccggc agcgggaact tgcccaggac gggaacgagt
     1681 ttgctcgcat cacaaataca gatgaacttg ccggccaccg ccgccacgat cttctcgcgc
     1741 gtcagtgcac cgccgccgcc cttgagcatg tgaaaatgtt cggtgatttc atcggcaccg
     1801 tcgacataga ttcccatatc gcccacgttc accgagttca agtccaacac ctcgataccg
     1861 tgcccacgta agcgctgcgc agtagattcg gagctggcca ctgcaccgtc gatcttgtgc
     1921 ttgaccttgg ccagttcctc gatgaaatag ttggcggtgg agccggtacc aacaccgatg
     1981 atgccgcctc tgacatattc agcagcagcc ctggccacct gctgtttgag ttcatcctgc
     2041 gttgccattc tttacccttt cacgatggtg ttatctttat aattgccaaa ttctaaaggt
     2101 ttaatgatac acgcccaccc gccaatgaaa aacgactatc tggaaatgat cctcaccgcc
     2161 cgcgtttacg atgttgccgt ggaaacccta ctggaagcgc aacccaatct gtcgcgccgc
     2221 atcggcaaca cggtgctgct caagcgcgag gacatgcagc cggtgttctc gttcaaactg
     2281 cgcggcgcct acaacaaaat ggcgcatctc actgctgccc agttgaagcg aggcgtcatt
     2341 gccgccagcg ccggcaatca tgcccagggc gtagcgcttg ctgcgcagaa gctggggtgc
     2401 cgcgccgtca tcgtgatgcc gactaccaca ccgcagatca aggtcgaagc agtcaggaac
     2461 cgcggcgccg aggtggtgct ggcgggcgac tcttattccg atgcctacac tcatgcgctg
     2521 gcactggaaa agaaagagaa actcaccttc gttcatcctt acgacgaccc cgatgtcatc
     2581 gccggccagg gcaccatcgc catggaaatt ctgcgcgatc atcccgaccc tattcatgcc
     2641 atcttctgct gtgtcggcgg cggcggcctg attgccggta tcgccgccta cgtcaagcgc
     2701 ctgcgcccgg aaatcagggt gatcggcgtc gaggccagcg atgccgacgc gatgagccag
     2761 tcgctggtca agggtcaccg cgtgcgcctc aaccaggtcg ggctgttcgc cgatggcgcg
     2821 gcggtcaaga tcgtcggcga ggaaaccttc cgcctgtgcc gcgaatatgt cgacgaaatg
     2881 gttcaggtcg acaccgatgc catctgcgcg gcgatcaagg atgtcttcga ggacacccgc
     2941 tcgatcctcg agccggccgg cgcgctggcc atcgccggcg ccaaggcctg ggcggcgcgg
     3001 cacaagctgc gcaatgaaac gctggtggcg gtggcttccg gcgccaacac caactt
//
LOCUS       DAOMFA010000170         3013 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_4684_length_3013_cov_5.448614, whole genome shotgun sequence.
ACCESSION   DAOMFA010000170 DAOMFA010000000
VERSION     DAOMFA010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 3013)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3013)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3013
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4684_length_3013_cov_5.448614"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1466)
                     /locus_tag="PKW44_07815"
     CDS             complement(<1..1466)
                     /locus_tag="PKW44_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/oxaloacetate carboxyltransferase"
                     /protein_id="HQR51527.1"
                     /translation="MAKVLVSELVLRDGPQSMIATRLRTDDMLPICAKLDAIGFWSVE
                     AWGGATFDACVRFLKEDPWERLRKLRAALPNSRIQMLLRGQNLLGYRHYSDDVVRAFV
                     QKSADNGVDVFRIFDAMNDMRNIRVSVEAVKKVGKHAEGAISYTTSPVHDVAHFVSLA
                     REMAGMGCDTIAIKDMAGLLTPTATGELVRALRAAVSLPIHLHSHATSGLAAMSFLRG
                     VEAGATMLDTCNSAFGEGASHPSTESIVAALQGTEYDTGLDLAALQEITAYFREVRKK
                     YWQFESDFTGVDTRVLVNQVPGGMISNLSNQLKEQGALDKMDAVLAEIPHVREDLGFP
                     PLVTPTSQIVGTQSVLNVLTGTRYKSVTTEVKNYLLGHYGKAPGTVNDEVKRMAVGNA
                     EVITCRPADLLEDEMANLRVQSENLAKSEEDVLTYAMFPELAQTFLQERNAGSLKAEP
                     LLPKEAAVPTSARYAPNEFKVTLHGETFHIKLTGSGHHG"
     gene            complement(1495..2913)
                     /locus_tag="PKW44_07820"
     CDS             complement(1495..2913)
                     /locus_tag="PKW44_07820"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013148028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HQR51528.1"
                     /translation="MIKKILVANRGEIAVRIVRACSEMGIKSVAIYTDADRHALHVKK
                     ADEAYNIGSDPVVGYLNAHNIVNLAVASGCDALHPGYGFLSENPELAEICARRDIKFI
                     GPDADVIRQMGDKIQARNAMIAAGIPCVPGSDGNLEDVEEAVELAREIGYPVMLKATN
                     GGGGRGIRRCNSEKELLANYERVISEASKAFGKPEVFLEKCVVNPRHIEVQVLADSHG
                     NVIHLFERDCSIQRRNQKLIEIAPSPQLSQAQREYIGSLGVKAAKAVGYENAGTVEFL
                     LDQDNNFYFMEMNTRLQVEHTVTETITGIDIVQEQIRIADGLELQYKQDDVAFRGYAM
                     EFRINAEDPKNGFLPSFGKITRYYAPGGPGVRIDAAMYSGYVIPPYYDSMCAKLTVWA
                     LTWEGVIERGRRALSDMVVYGVKTTMPYYQEILKHPDFRGADFDTSFVESHPELINYA
                     TEFPPELVAAAISAAIAAHEGI"
BASE COUNT          526 a          853 c          987 g          647 t
ORIGIN      
        1 ccatgatggc cgctgccggt gagcttgata tggaaggttt cgccatgcag cgtgaccttg
       61 aattcgttcg gcgcatagcg cgcagaagtc gggacggcag cttccttggg cagcagcggc
      121 tcggccttga ggctaccggc gttgcgttcc tgcaggaagg tttgcgccag ctcggggaac
      181 atggcgtagg tcagcacgtc ttcctcgctc ttggccaggt tttccgactg cacgcgcagg
      241 ttggccattt cgtcttccag caggtcggcg gggcgacagg tgatgacctc ggcattgccg
      301 accgccatgc gcttgacttc gtcgttgacg gtgccgggcg ccttgccata gtggccgagc
      361 aggtagttct tgacctcggt ggtgaccgat ttgtagcgcg tgccggtgag cacgttgagc
      421 accgactggg tgccgacgat ctgcgaggtg ggcgtgacca gcggcgggaa accgaggtct
      481 tcgcgcacgt gcgggatttc cgccagcacg gcgtccatct tgtccagcgc gccctgttcc
      541 ttgagttggt tggacaggtt ggagatcatg ccgcccggta cctgattgac gaggacgcgg
      601 gtatccacgc cggtgaaatc gctctcgaac tgccagtatt tcttgcgcac ctcgcggaag
      661 taggcggtga tttcctgtag cgcggccaga tcgaggccgg tgtcgtattc ggtgccctgc
      721 agcgcggcga cgatgctttc ggtggagggg tggctggcac cttcaccgaa ggccgagttg
      781 caggtgtcca gcatggtggc gccggcttcc acgcccctga ggaagctcat ggccgccagg
      841 ccagaagtgg cgtggctgtg caagtggatg ggcaggctca ccgcggcgcg cagcgccctg
      901 accagttcgc cggtggcggt aggggtgagc aggccggcca tgtccttgat ggcgatggtg
      961 tcgcagccca tgcctgccat ttctctggcc aggctgacga agtgggcgac gtcgtgcacc
     1021 gggctggtgg tgtagctgat ggcaccctcg gcgtgcttgc cgaccttttt caccgcttcc
     1081 accgagacgc ggatgttgcg catgtcgttc atggcgtcga agatgcggaa cacgtccacg
     1141 ccgttgtcgg cggatttctg cacgaaggcg cgcaccacgt cgtcggagta atggcggtag
     1201 ccgagcaggt tctggccgcg cagcagcatc tggatgcggc tgttgggcag ggcggcgcgc
     1261 agcttgcgca ggcgttccca cgggtcttct ttcaggaatc gcacgcaggc gtcgaaagtg
     1321 gcgccacccc aggcttcgac tgaccagaag ccgatggcat ccagtttggc gcagatcggc
     1381 agcatgtcgt cggtgcgcag gcgcgtggcg atcatggact gcgggccgtc acgaagaacc
     1441 aattcagaaa caaggacttt tgccatagta ttaatctagt tatttaagtt aaaatcaaat
     1501 gccttcgtgc gcggcgatgg cggcggatat ggctgccgcc accagctccg gcgggaactc
     1561 ggtggcgtag ttaatcagtt cgggatgcga ttcgacgaag ctggtgtcga aatcggcgcc
     1621 ccggaaatcc ggatgcttga ggatttcctg atagtacggc atggtggtct tcacaccata
     1681 caccaccatg tcgctcagtg cgcgacggcc gcgctcgatc acaccttccc aagtcagtgc
     1741 ccagacggtc agcttggcgc acatggaatc gtaatagggc ggaatgacgt agccgctata
     1801 catggcggcg tcgatgcgca cgccggggcc gcccggcgcg taataacggg tgatcttgcc
     1861 gaagctgggc agaaaaccgt tcttcgggtc ttcggcattg attctgaatt ccatggcgta
     1921 gccgcggaat gcgacgtcat cctgcttgta ttgcagttcc aggccgtcgg cgatgcgaat
     1981 ctgctcctgc acgatgtcga tgccggtaat ggtttccgtt accgtgtgct cgacttgcag
     2041 gcgggtgttc atttccatga agtagaagtt gttgtcctgg tcgagcagga actctaccgt
     2101 gccggcgttt tcgtagccca cggccttggc ggccttgacg cccaggctac cgatgtattc
     2161 gcgctgtgcc tgactcaact gcggcgaagg ggcgatttcg atcagcttct ggttgcggcg
     2221 ctggatggag cagtcgcgtt caaacaaatg aatgacgttg ccatgactgt cggccagcac
     2281 ctggacttca atatggcgcg ggttgaccac gcacttttcc aggaatactt cgggcttgcc
     2341 gaaggccttg ctggcttcgg aaatcacgcg ctcgtaattg gcgagcagtt ctttttcgct
     2401 gttgcagcgg cgaatgccgc gcccgccacc gccgttggta gccttgagca tcaccgggta
     2461 gccgatttca cgggccagct cgaccgcctc ctcgacgtcc tccaggttgc cgtcgctgcc
     2521 cggcacacag gggatgccgg cggcgatcat ggcattgcgc gcctggatct tgtcacccat
     2581 ctggcggatc acgtccgcat cgggaccgat gaacttgatg tcgcggcgtg cgcagatttc
     2641 cgccagttcc gggttttccg agagaaagcc gtagccggga tgcagcgcat cgcagccgga
     2701 tgcgacagcc agattgacga tgttgtgggc attaagatag ccgaccaccg ggtcagagcc
     2761 gatgttgtag gcctcgtcag cctttttgac atgcagcgcg tggcgatcgg catcggtata
     2821 gatggcgacc gacttgatgc ccatttccga gcaagcccgg acgatgcgga cggcaatttc
     2881 accacggttg gcaacgagga ttttcttgat cacgaaatag ggcgtgttag cattaatttc
     2941 gcgtctgcga cgggggcgat ttgggatgcc atccgcgaag caagacgcgt cgaattgcaa
     3001 gccacttcaa gcg
//
LOCUS       DAOMFA010000171         2986 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_4746_length_2986_cov_5.084613, whole genome shotgun sequence.
ACCESSION   DAOMFA010000171 DAOMFA010000000
VERSION     DAOMFA010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 2986)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2986)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2986
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4746_length_2986_cov_5.084613"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..106
                     /locus_tag="PKW44_07825"
     CDS             <1..106
                     /locus_tag="PKW44_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008059199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fructose-bisphosphatase class I"
                     /protein_id="HQR51529.1"
                     /translation="QRMLDVEPQALHQRVPVILGSRNEVEELVSYHQT"
     gene            complement(97..1548)
                     /locus_tag="PKW44_07830"
     CDS             complement(97..1548)
                     /locus_tag="PKW44_07830"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018507321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003904 - deoxyribodipyrimidine
                     photo-lyase activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HQR51530.1"
                     /translation="MPDHLYQTSLVWFRRDLRDYDHAALYHALKSSQQVYCVFIFDTE
                     ILDRLADKADRRVEFIWESVRELKSALRDKGGDLIVLHGAARDEIPALAQRLGAQAVY
                     ANRDYEPQAVARDAAVTSALAAQGVAFRQLKDQVIFECDEVLTQAGKPFGVFTPYRNA
                     WLKRLTAIDLEPLVVETHESALARSDDFHTTLPTLEQLGFRRTNLLDLMPAGMSGAER
                     LFESFQERIDDYHTARDYPALKGPSYLSVHLRFGTLSIRTVASHAWYMGGRGAETWLS
                     ELIWREFYFMLLHYHPRLMEGHCFKQEFDNIRWPGSEAHFQAWCAGRTGYPLVDAAMR
                     QLDQSGYMHNRLRMVTASFLVKDLLVDWRKGEAWFADKLLDFDFAANNGGWQWAASTG
                     CDAQPWFRIFNPVTQSEKFDPRGKFIRKYVPELAGCNDKEIHAPWQIPLERLRELNLE
                     IGRDYPAPLVSHALQRQRALALYKTPASNKSYV"
     gene            complement(1541..2014)
                     /gene="rlmH"
                     /locus_tag="PKW44_07835"
     CDS             complement(1541..2014)
                     /gene="rlmH"
                     /locus_tag="PKW44_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HQR51531.1"
                     /translation="MKIRIIAVGHKMPAWVEQGCAEYLKRMPRETAVEIVELRPDKRA
                     AGKGGEQVREAEAQRILEATGRDFLIALDERGQEPSTLQLADRMQQWLADGRDVALAI
                     GGADGLHDKVKQAAHWQWSLSRLTLPHGMVRVLLAEQLYRAWSVLQNHPYHRGDA"
     gene            complement(2011..2370)
                     /gene="rsfS"
                     /locus_tag="PKW44_07840"
     CDS             complement(2011..2370)
                     /gene="rsfS"
                     /locus_tag="PKW44_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HQR51532.1"
                     /translation="MELEAMKQAVVDALEDIKATDISVLDVSKLTSITSYMIIASASS
                     SRQAKALAHSVQEKLKELGVAITGVEGEREGEWVLVDLGDIIVHIMLQGTRDYYNLEQ
                     LWGAAEGRRQTAKPAAA"
     gene            complement(2360..>2986)
                     /gene="nadD"
                     /locus_tag="PKW44_07845"
     CDS             complement(2360..>2986)
                     /gene="nadD"
                     /locus_tag="PKW44_07845"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HQR51533.1"
                     /translation="PLHFGHLRVAQKLYDELHLANVHFGPAASPPHRDQPQTTTTHRV
                     AMTRLAIATNPAFTLDTRELGRPGPSYTFDTLSELRAELGADKPLCLLIGGDAFLGLP
                     TWHRWRELLAHAHIVVAHRPGAVPSEAAMSEELRALWRQHGTDNPAHLHASPGGSILL
                     HRITALDISASSIRASLRQGGSVRYLLPDAVADFIQTYHLYQKEPHGT"
BASE COUNT          500 a          915 c          979 g          592 t
ORIGIN      
        1 ccagcgcatg ctcgatgtcg agccgcaggc gctgcaccag cgtgtgccgg tcattctcgg
       61 ctccaggaac gaagttgagg agctggtctc ataccatcag acgtaggatt tgttactggc
      121 tggcgtttta tataatgcga gagctcgctg ccgctgcagt gcatgactca caaggggggc
      181 agggtagtcc ctgccgattt ccaggttcag ttcgcgtagc cgctcaagcg gtatctgcca
      241 cggggcgtgg atttccttgt cattgcaccc cgccagttcc ggcacgtatt tgcggatgaa
      301 cttgccgcgc gggtcgaatt tctccgactg cgtcaccggg ttgaaaatgc ggaaccacgg
      361 ctgagcatcg cagccggtgg aagctgccca ctgccagccg ccgttgttgg cggcaaaatc
      421 gaagtccagc agcttgtcgg cgaaccaggc ctcgcccttg cgccagtcca ccagcaagtc
      481 cttgaccaga aaactggcgg tcaccatgcg caggcggttg tgcatgtagc cgctctggtc
      541 gagctggcgc atggcggcat ccaccagcgg gtagccggta cgacctgcgc accaggcctg
      601 gaaatgcgct tcgctgcccg gccagcggat attgtcgaac tcctgcttga agcagtgccc
      661 ttccatcagc cgcggatgat agtgcagcag catgaaatag aactcgcgcc agatcagttc
      721 gctcagccac gtctcggcac cgcgcccacc catgtaccag gcatggctcg ccacggtgcg
      781 gatggatagc gtgccgaagc gcaggtgcac cgagaggtag gacggccctt tgagcgccgg
      841 gtagtcgcgt gcggtgtggt agtcgtcgat gcgttcctgg aagctctcga acagccgctc
      901 cgcgccggac atgccggcgg gcatcaggtc gagcaggttg gtgcgccgga agccgagctg
      961 ctccagcgtg ggcagggtgg tatggaagtc gtcgctgcgt gccagggcgg attcgtgtgt
     1021 ttccaccacc agcggttcga ggtcgatggc ggtcagccgt ttcagccagg cattcctgta
     1081 cggcgtgaac actccgaacg gcttgccggc ttgggtaagc acctcgtcac attcaaaaat
     1141 cacctgatcc ttgagctggc ggaaggcaac gccctgagcg gcaagtgcag aggtcacggc
     1201 ggcatcgcgt gccaccgcct gcggctcgta gtcacggttg gcatacaccg cctgcgctcc
     1261 cagccgctgc gccagcgccg ggatctcgtc gcgcgccgcg ccatgcagca cgatcaggtc
     1321 gccacccttg tcgcgcaggg cggatttcag ctcgcgcacg ctttcccaga tgaattcgac
     1381 gcgccggtcg gccttgtcgg caagccggtc aagaatctcg gtgtcgaaga tgaatacgca
     1441 gtaaacctgt tgggatgact tcagtgcgtg gtatagcgcc gcgtggtcgt agtcgcgaag
     1501 atcgcggcga aaccagacca gggatgtttg ataaaggtga tcaggcatcg ccgcggtggt
     1561 agggatggtt ctgtagcact gaccacgcac ggtataactg ctccgccaac agcacccgta
     1621 ccatgccgtg cggtagtgtc aggcgcgaca aactccattg ccagtgggcg gcttgcttga
     1681 ccttgtcgtg caggccgtcg gcgccgccga tagcgagcgc cacgtcgcgt ccatcggcca
     1741 gccattgctg catcctgtcg gcgagttgca atgtggatgg ctcctgtccg cgctcgtcca
     1801 gcgcgatcag aaaatcacgc cccgtggctt ccaggatgcg ctgggcttca gcttcccgca
     1861 cctgctcgcc gcccttgccg gcggcgcgct tgtcgggcct gagttcgacg atctccactg
     1921 cggtttcgcg cggcatgcgc ttcaggtact cggcgcatcc ttgttccacc caggccggca
     1981 ttttgtggcc gaccgcgata atccggattt tcacgcggct gcgggtttgg cagtctgacg
     2041 gcggccttcg gcggcgcccc agagctgttc caggttgtag tagtcgcgcg tgccttgcag
     2101 catgatgtgg acgatgatgt cgccaaggtc caccagcacc cactcgccct cgcgctcgcc
     2161 ctctacgccg gtgatcgcca cgcccagttc cttcagcttt tcctgcacgc tgtgggccag
     2221 ggccttggcc tgtcggctcg agctggcgct ggcgatgatc atataggaag tgatcgaggt
     2281 gagtttgctg acgtccagca cgctgatgtc ggtggctttg atgtcttcca gcgcgtcgac
     2341 gacggcctgt ttcatggctt caagttccat ggggttcctt ctggtagagg tgatacgttt
     2401 gaatgaaatc ggcaaccgcg tccggcagca ggtagcgtac gctgccccct tgacgcaagc
     2461 ttgcgcgaat actgctggcg gagatatcca gtgcagtaat gcggtgcagc aggatgctgc
     2521 cacctggcga cgcgtgcagg tgggcaggat tatccgtgcc gtgctgtcgc cacaaagcgc
     2581 gcaattcttc ggacatggcg gcttcgctcg gaacggcgcc ggggcggtgg gcgaccacga
     2641 tgtgcgcgtg tgccagcagc tcccgccagc ggtgccaagt cggcaggccg aggaaggcat
     2701 cgccgccgat cagcaggcac agcggcttgt ccgcccccag ctccgcgcgc agctcgctca
     2761 gggtatcgaa agtgtaggac ggccccggcc ggcccagttc gcgtgtgtcc agcgtgaagg
     2821 cgggatttgt ggcgatcgcc agccgggtca tggctacgcg atgggttgtt gtggtctgcg
     2881 gctggtcgcg gtgcggcggg ctggcggcgg gcccgaagtg cacgttcgcc aggtgcagct
     2941 catcgtacaa tttctgcgcc acgcgcagat ggccgaaatg gagggg
//
LOCUS       DAOMFA010000172         2979 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_4768_length_2979_cov_5.925103, whole genome shotgun sequence.
ACCESSION   DAOMFA010000172 DAOMFA010000000
VERSION     DAOMFA010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 2979)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2979)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2979
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4768_length_2979_cov_5.925103"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..359)
                     /locus_tag="PKW44_07850"
     CDS             complement(<1..359)
                     /locus_tag="PKW44_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HQR51534.1"
                     /translation="MAGKTLYDKLWESHVVREEADGTCLLYIDRHLVHEVTSPQAFEG
                     MRLAGRKPWRNTSIVANPDHNTPTDHWERGIEDPVSRLQVETLDQNIKDFGALMYFPL
                     KHANQGIIHVTGTAHGTP"
     gene            478..1266
                     /locus_tag="PKW44_07855"
     CDS             478..1266
                     /locus_tag="PKW44_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HQR51535.1"
                     /translation="MDTRRKELGEFLQAMRQRGSPEEFGFPSGNRRRTAGLRREEVAQ
                     LAGISATWYTWIEQGREVNVSAEALDRLAQALKLSRPERGYLFDMADRRDPRSSETEE
                     DSAPQVLVGMLSSIAIPAYIMGRTWDLLAWNPPAAELFAGWLDQKTTEAPAPNLLRYV
                     FQHPDARQFVVNWEVRARRLVAEFRADCRGRLEEPALQRLVNELSQASPDFERYWKQH
                     DVLERQGGQREFNHPQQGLISYQQVTLRPVDQEQIKLVLLTPAD"
     gene            complement(1235..2836)
                     /gene="cimA"
                     /locus_tag="PKW44_07860"
     CDS             complement(1235..2836)
                     /gene="cimA"
                     /locus_tag="PKW44_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018113690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HQR51536.1"
                     /translation="MARVIAYDSTLRDGAQAQGVSFSVEDKLKIVERLDGLGIAYIEA
                     GNPGSNPKDLEFFSRVAGLKLKHARIIAFGSTRKVGIAAEEDKNLQSLLHANTSAVAI
                     FGKSWDYQVTGILRTTLEENLRMIADTITFLKGQGKEVVFDAEHFFDGYKANADYAMQ
                     SLQAAADAGADVLCLCDTNGGTFPDQIHAITRAVVERFSVQVGIHCHNDCEMAVANSV
                     AAVQAGAVQVQGTINGIGERCGNANLCSIIPSLQLKLGLDCIPAQNMAHLTSVARFVS
                     EVANVTLNDKAPYVGNDAFSHKGGMHIDAVSKNPISYEHINPEQVGNVRHILVSEVAG
                     RSALLAKINAVDASLSKDSPDTKRILDHLKALEHEGYQFESAESSFELLVYKLLGKYQ
                     PFFELKEYTVVIHEPAVNGINSTATIRIVVDGQEENTIADGDGPVNALDKAMRRALER
                     FYPAIREVKLTDYKVRVLDSDKASAAKVRVLIESADHIESWTTIGVSTDIIDASWRAL
                     IDSIEYKLMRDQERASSQPVSAAPA"
     gene            complement(2836..>2979)
                     /locus_tag="PKW44_07865"
     CDS             complement(2836..>2979)
                     /locus_tag="PKW44_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018985120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HQR51537.1"
                     /translation="KTDSLFDPTIATFEDDAGAYDQKDAGGFIKLNALRMRIAANLRN
                     RKK"
BASE COUNT          564 a          861 c          930 g          624 t
ORIGIN      
        1 ggggtgccgt gtgccgtgcc ggtgacgtgg atgatgccct ggtttgcatg ctttaacggg
       61 aaatacatca gcgcgccgaa atccttgatg ttctggtcca gggtttccac ctgcaggcgg
      121 gaaaccggat cttcgatgcc gcgctcccaa tggtcggtcg gcgtgttgtg gtcggggttg
      181 gccacgatgg aagtattgcg ccagggcttg cggccggcca gtcgcatacc ttcaaaagcc
      241 tgcgggctgg tcacctcgtg caccagatgg cgatcgatat agagcaggca agtaccgtcc
      301 gcttcctcgc ggaccacatg cgattcccat aacttgtcgt agagggtttt tccagccata
      361 acagctcctt gaaaaaatac gctaaagatt gaatgtgggc gaagaataaa cactttctca
      421 taccgttaca agaatagtag ttatactagt actgtaggta acactttagg agttggaatg
      481 gataccagac gcaaggaatt aggcgagttt ctgcaggcga tgcggcaacg cggctcgccg
      541 gaggaattcg gctttccttc gggaaatcgc cgccgcacgg cgggcctgcg gcgtgaggaa
      601 gtcgcccagt tggccggcat cagcgccacc tggtacacct ggatcgaaca ggggcgcgag
      661 gtcaacgtct cggcagaagc gctcgaccgg ctggcgcagg cattaaagct ctcgcgcccg
      721 gaacgcggct acctgttcga catggccgac cgccgcgacc cgcgcagcag cgaaaccgaa
      781 gaagacagcg cgccccaagt gctggtcggc atgctctcca gcatcgccat ccccgcctac
      841 atcatgggtc gcacctggga cttgctggca tggaacccgc ccgccgccga gctgtttgcc
      901 ggctggctgg accagaaaac cacagaggct cccgccccca acctgctacg ctacgtgttc
      961 cagcaccccg acgcgcgcca gttcgtagtg aattgggaag tgcgggcaag aagactggtg
     1021 gcggagttcc gcgccgattg ccggggcagg ctggaagagc cggcgctgca gcggctggtc
     1081 aacgagctat cgcaagccag cccggatttc gaacgttact ggaaacagca cgacgtgctg
     1141 gagcgccagg gcgggcaacg ggaattcaac catccacagc agggactcat cagctatcag
     1201 caagtaacct tgcgtccagt ggatcaggaa cagatcaagc tggtgctgct gacaccggct
     1261 gactagatgc ccgttcctgg tctcgcatca gcttgtattc gatggaatca atcagcgcgc
     1321 gccatgaggc gtcgatgatg tcagtcgaaa cgccgatggt cgtccagctt tcgatgtggt
     1381 cggcagattc gatcagtacg cgcaccttgg cggcggaggc tttgtcggaa tcgagtacgc
     1441 gcaccttgta gtcggtgagt ttgacttccc tgatcgccgg gtaaaagcgt tccagcgcgc
     1501 ggcgcatggc cttgtccagc gcgttgaccg ggccgtcgcc gtcagcgatg gtgttttctt
     1561 cctgcccgtc caccacgatc ctgatggtgg cggtggagtt gatgccgttg acggccggtt
     1621 cgtggatgac gacggtgtat tccttcagct caaagaacgg ctggtacttg ccgagcagtt
     1681 tgtacaccag cagttcgaac gagctttcgg cgctctcgaa ctggtaacct tcgtgctcca
     1741 gcgccttgag gtggtcgagg atgcgcttgg tgtccggcga atccttgcta agcgaggcgt
     1801 ccaccgcgtt gatcttggcc agcagtgcac tgcgcccggc gacttccgac accaggatgt
     1861 ggcgcacgtt gccgacctgc tccgggttga tgtgttcgta ggagatcggg tttttgctca
     1921 ccgcgtcgat gtgcatgccg cccttgtgcg agaaggcgtc gttgccgaca tagggcgcct
     1981 tgtcgtttaa ggtgacgttg gcaacttcgc tgacaaagcg cgccaccgaa gttaggtgcg
     2041 ccatgttctg cgccgggatg cagtcaaggc ccagcttgag ttgcaggctg ggaataatcg
     2101 aacacaggtt ggcgttgcca cagcgctcgc cgatgccgtt gatggtgccc tgcacctgca
     2161 ccgcgccagc ctgcaccgcc gccacggaat tggccacggc catttcgcag tcgttatggc
     2221 aatggatgcc gacttgtacg ctaaagcgtt caaccaccgc tctggtgatg gcgtgaatct
     2281 ggtcagggaa agtgccgccg ttggtatcgc acaggcacag cacatcggcg ccggcatctg
     2341 ccgccgcctg caggctttgc atggcgtaat cggcgttggc cttgtagccg tcgaagaagt
     2401 gctcggcgtc gaacaccact tccttgccct gccctttcag gaaagtgatg gtatcggcga
     2461 tcatgcgcag gttttcttcc agcgtggtgc gcaggatgcc ggtgacctgg taatcccagc
     2521 tcttgccgaa aatggccact gcgctggtat tggcgtgcag cagcgattgc aggttcttgt
     2581 cttcctcggc ggcgatgccg accttgcgtg tgctgccgaa agcgatgatc ctggcgtgtt
     2641 tcagcttgag tccggccacc cgcgagaaga actccaggtc cttggggttg gaacccggat
     2701 tgccggcctc gatgtaggcg atgccaagac cgtccagccg ctcaacgatc ttgagcttgt
     2761 cttccaccga gaaggaaacg ccctgtgcct gcgcgccgtc gcgcagggtg gagtcatagg
     2821 caataacccg cgccattact ttttgcggtt gcgcaagttg gcggcaatac gcatgcgcag
     2881 tgcattcagc ttgatgaagc cgcccgcatc cttctggtcg taggcgccgg catcgtcctc
     2941 gaaggtggcg atggtcggat cgaacagcga atcggtctt
//
LOCUS       DAOMFA010000173         2937 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_4883_length_2937_cov_3.912561, whole genome shotgun sequence.
ACCESSION   DAOMFA010000173 DAOMFA010000000
VERSION     DAOMFA010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 2937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2937
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4883_length_2937_cov_3.912561"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..266
                     /locus_tag="PKW44_07870"
     CDS             <1..266
                     /locus_tag="PKW44_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidohydrolase family
                     protein"
                     /protein_id="HQR51538.1"
                     /translation="HGAVSEQTAREMARGALENSHAQVTAAITGIAGPDGGTPDKPVG
                     TVCFAWADATGRITSATLHFTGDREAVRRQSVDHCLNTMLRMR"
     gene            complement(294..701)
                     /gene="arfB"
                     /locus_tag="PKW44_07875"
     CDS             complement(294..701)
                     /gene="arfB"
                     /locus_tag="PKW44_07875"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HQR51539.1"
                     /translation="MPSEILIPDSEIEITAIRAQGAGGQNVNKVSSAVHLRFDIRASS
                     LPEAVKERLLRLRDQRITAEGVVVIKAQRYRSQELNRQDAIARLHALVSRAARVPVVR
                     RATKPTLGSQKRRVEGKVQRGRIKSLRGKVGVD"
     gene            1053..1448
                     /locus_tag="PKW44_07880"
     CDS             1053..1448
                     /locus_tag="PKW44_07880"
                     /inference="COORDINATES: protein motif:HMM:NF015146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HQR51540.1"
                     /translation="MLHLLRALQMDPGAVPASNIVFLRSRREDSIDGNLEELAEACWP
                     FHRRVIEELNVRTVLCFGKTAGKWVCNHLGANKLIGQFVEQNNRRWKSAAFCNTDGIK
                     VVVGTHPSIADWTSPTTDPSELVRQAIHG"
     gene            complement(1785..>2937)
                     /locus_tag="PKW44_07885"
     CDS             complement(1785..>2937)
                     /locus_tag="PKW44_07885"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HQR51541.1"
                     /translation="LPEPTTALEKAVRDFRLLFGLEGARMPVQRALERSGFSEAYDGM
                     AGKLKELAAVLETQAERDPALENCWQRALALEAQLRSWQAGANANLVRWVEVFTQSVQ
                     LHATPLSIAEIFGKQMEGHPRAWIFTSATLAVKNDFGHYLGQMGLPQAVTARWDSPFD
                     YPSQSVLYVPHGMPPPNSPGYIPAVAALALPAIQASRGRAFVLCTSLRAMRDIHALLK
                     DAFAREGLDYPLLLQGESSRTELLDRFRRLGNAVLVGSQSFWEGVDVRGEALSLVVID
                     KLPFAPPDDPVLAARIDKMNAEGKNAFMEYQLPYAVITLKQGAGRLIRDETDRGVLMI
                     CDPRLVEKHYGRRIWQSLPPMKRTREVGEVEAFFATVRPELVEGQGSES"
BASE COUNT          577 a          924 c          883 g          553 t
ORIGIN      
        1 cgcacggcgc ggtgagcgaa cagaccgccc gcgagatggc ccgcggcgcg ctggaaaaca
       61 gccacgccca ggtcaccgca gcgattaccg gcatcgcggg gccggacggc ggcacaccgg
      121 acaaaccggt cggcacggtc tgcttcgcct gggcggacgc gacgggcagg atcaccagcg
      181 ccaccctgca cttcaccggc gaccgggaag cggtgcgccg gcaatcggtg gatcactgcc
      241 tgaacacgat gctgcgcatg cggtgagctt gcacacgccg cgacgcagcc ccgctaatcc
      301 acccccacct tgccgcgcag agacttgatg cgcccgcgct gcaccttgcc ctccacccgc
      361 cgcttctgcg aacccaaagt cggctttgtc gcccgccgca ccaccggcac tcgcgccgcc
      421 cggctcacca gcgcatgcag ccgcgcaatc gcatcctgcc ggttcaactc ctggctgcgg
      481 tagcgttgcg ccttgatcac gaccacaccc tcggccgtga tgcgctggtc gcgcagcctc
      541 agcaaccgtt ccttcaccgc ctccggcaac gacgacgccc ggatatcgaa gcgcaaatgc
      601 accgcgctcg aaaccttgtt gacgttctgc ccgcccgctc cctgcgcacg gatggccgtg
      661 atctcgattt cgctgtcggg aatgaggatt tcggagggca tgttgggtag agattatgtt
      721 cccgattgct tctcaggcaa atatgcgaag attatgtcgt ctatttaacg tttgtaagct
      781 ccggcatgat cgacactttg aagcaattaa ttcccgaaga ccttatggat cgatcaggct
      841 ctgtctttta ctccggtaga gacgcatttt ctagcacgcg gccactctac ctgctcggcg
      901 tgaatcccgg aggtgaccct tctataaagt ccgaagagac attgcgttgg catacaaaca
      961 aagtgcttca cgaggaacca gataattggt ccgcgtatcg tgacgagtcg tgggagggcg
     1021 ctccgccagg tacctggaga atgcagcccc gagtgttgca tcttctgcgt gccctccaaa
     1081 tggacccggg tgcagtacct gccagcaaca ttgtgttctt gaggtcgcga cgggaagaca
     1141 gcattgatgg aaatcttgaa gaattagctg aggcctgctg gccatttcat cgtagagtta
     1201 tcgaagaatt gaatgtgaga acggttttat gctttggaaa aaccgcaggg aaatgggtgt
     1261 gtaatcacct tggcgctaat aaacttatcg gccagtttgt tgaacaaaat aatcgtcgct
     1321 ggaaaagcgc agccttttgc aatactgacg ggatcaaggt tgttgtggga acgcatccaa
     1381 gcatcgccga ctggacttcg cctacaacag acccgagcga acttgtgcgt caggcaatcc
     1441 atggttaact cagatcggaa ttgcggctat gagcaagcgg agggtgggca aaggctgtca
     1501 agccgtgccc acgcggttaa aatggtgagc acgctatgct ttgcccaccc tacagccaaa
     1561 ggttaaacca ccaccccatt cccctgctgc cgcgccgagc agcacagcca agacgggagt
     1621 agtcggcggg cgttgtctga gcgcagcgag tttagcccgt cgcccggctt ggcgagcagc
     1681 acagggaacc cgaagggcgc ggcagggggt gcccttcttc tttggctact ttcttttggg
     1741 caagcaaaag aaagtagctc ggctgtcggc cgagaccgac ggctttagct ttctgagccc
     1801 tgcccttcga caagctcagg gcgaacggta gcgaagaacg cctccacctc ccccacctcc
     1861 cttgtcctct tcatcggcgg caagctctgc caaatgcgcc gtccatagtg cttctccacc
     1921 agccgcgggt cgcagatcat cagcaccccg cgatccgtct cgtcgcggat caatcgcccc
     1981 gccccctgct tgagggtgat gacggcatag ggcagctggt attccatgaa ggcgttcttg
     2041 ccttccgcgt tcatcttgtc gatgcgcgcg gccagtacag gatcgtccgg cggggcgaac
     2101 ggcagcttgt cgatgaccac cagcgacagc gcctcgccgc gcacgtccac gccttcccag
     2161 aagctttgcg agcccaccag tacagcgttg cccagccggc ggaagcggtc aagcagctcg
     2221 gtacgcgagc tctcgccctg taacagcaag ggataatcca gcccctcacg cgcaaaggcg
     2281 tccttgagca gggcgtggat gtcgcgcatg gcacgcaggc tggtgcacag cacgaaggcg
     2341 cggccacggc tagcctggat ggcgggcagc gccagcgccg ccacggcagg gatataaccg
     2401 gggctgttgg gcggcggcat gccatgcggc acgtagagca cggactggct ggggtagtcg
     2461 aaaggactgt cccagcgcgc ggtgacggcc tgcggcaggc ccatctgtcc gaggtagtgg
     2521 ccgaaatcgt tttttacggc caacgtggcg gaggtgaata tccacgcgcg gggatggcct
     2581 tccatctgct taccgaagat ttccgcgatg gaaagcggcg tggcgtgcag ctgcaccgac
     2641 tgggtgaaca cctcgaccca gcgcaccagg ttggcgttgg cgccggcctg ccagcttcta
     2701 agctgggctt cgagggcgag cgcccgctgc cagcagtttt ccagcgcggg atcgcgctca
     2761 gcctgggttt ccagcacggc ggccagttcc ttgagtttgc ctgccatgcc atcgtaagcc
     2821 tcgctgaaac cgctgcgttc cagcgcccgc tgcaccggca tacgcgcgcc ttccaggcca
     2881 aacaacaggc ggaaatcgcg cacggctttt tccagcgcgg tggtgggctc gggcagc
//
LOCUS       DAOMFA010000174         2897 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_4987_length_2897_cov_5.134061, whole genome shotgun sequence.
ACCESSION   DAOMFA010000174 DAOMFA010000000
VERSION     DAOMFA010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 2897)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2897)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2897
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4987_length_2897_cov_5.134061"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(27..704)
                     /locus_tag="PKW44_07890"
     CDS             complement(27..704)
                     /locus_tag="PKW44_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HQR51542.1"
                     /translation="MHLIQRGNNRSACFYAEEDYRRYLDHLHELSPKFGCAIHAYVLM
                     TNHVHLLISGQAADSVSQLMKHLGQRYVQYINRSYKRSGTLWEGRYRSCLLQDEDYLL
                     RCHRYIEMNPVRANMVEHPADYKWSSYRYNAQGEPDKLISPHPQYLALGQIEESRLIA
                     YRELFRTDLDPHLVQQIREATNGGYALGDNRFREEVAQMLGRRVERGKAGRPKKELEP
                     TMEGAKQ"
     gene            complement(876..1328)
                     /gene="dut"
                     /locus_tag="PKW44_07895"
     CDS             complement(876..1328)
                     /gene="dut"
                     /locus_tag="PKW44_07895"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HQR51543.1"
                     /translation="MTLKVDLKLLDARLHHHQPSYATPGSAGLDLRACIDHVLTLHPG
                     ETELIPTGMAIHIADPGYAALILPRSGLGHKHGIVLGNLVGLIDSDYQGQLFVSCWNR
                     GRTPFILNPLERIAQLVIVPVTHADFNVVEEFPASKRGEGGFGSTGKH"
     gene            complement(1325..2542)
                     /gene="coaBC"
                     /locus_tag="PKW44_07900"
     CDS             complement(1325..2542)
                     /gene="coaBC"
                     /locus_tag="PKW44_07900"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HQR51544.1"
                     /translation="MAHSPVKRLLVGITGGIAAYKAAELVRLLVKQGVEVQVVMTQAA
                     CQFITPVTMQALSGKPVFTDLWDDRSGNGMAHIELARNADAILIAPASADFIAKLVHG
                     AADDLLSTLCLARGECPLLVAPAMNVQMWKNPATQRNVAQLINDGIAVLGPDSGDQAC
                     GETGLGRMLEPQALLDALETGPLPKLLVGRKVLLTAGPTFEAIDAVRGIANLSSGKMG
                     YAIARAALEMGAEVTLVSGKTSLPVPTGARLVQVTSARQMLAAVMDNLAGQDIFIGVA
                     AVADYCAPNPSEQKIKKNEQNLTLELVPTVDILASVAGLPNPPFCVGFAAESENLYEF
                     AEQKRQRKHLPLLAANLIQDALGGDDSELILLDDNGTHPLSRAPKSVLARQLLQHVVA
                     MLNEPKFATGRKP"
     gene            2592..>2897
                     /locus_tag="PKW44_07905"
     CDS             2592..>2897
                     /locus_tag="PKW44_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018986243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR51545.1"
                     /translation="MAITDWPEGERPREKLLAQGTQVLSDAELLAIFLRVGVTGKSAV
                     DLARDLLTEFGSLNGIFGASRAALTAVNGMGDSKYAQLQAIFEMSRRALEEQMRQRDA
                     "
BASE COUNT          542 a          840 c          886 g          629 t
ORIGIN      
        1 cagaccacgt ttttctgatg tcactttcat tgctttgctc cttccatagt tggctcaagt
       61 tccttcttcg ggcgccctgc tttacccctc tctacccttc gtcccagcat ttgggccacc
      121 tcttctctga atcggttgtc tcccaaggcg taacctccgt tcgtggcttc ccggatttgc
      181 tgtaccagat gcgggtcaag atcggttctg aagagttctc gataagcaat gagtctcgat
      241 tcttcaatct gcccaagcgc gaggtattgc gggtgcgggc tgatcagttt gtctggttcg
      301 ccttgggcgt tgtatctgta actgctccat ttgtagtccg ccgggtgctc taccatgttg
      361 gcccggacgg ggttcatttc gatgtagcgg tggcaacgta acaagtagtc ctcatcctgt
      421 agtaggcagg aacggtaacg tccttcccat agggtgccgc tgcgtttgta gctgcggttg
      481 atgtattgaa cgtaacgttg gccgaggtgt ttcatgagtt gcgatacgct gtcagccgct
      541 tgtccactga tgagcaggtg gacgtggttg gtcatgagta cgtaggcgtg aatggcgcag
      601 ccgaatttgg gggatagctc gtgcaggtga tcaaggtaac gccggtagtc ttcttcggcg
      661 tagaagcagg cgctgcggtt gttcccgcgc tggatcaggt gcaacggggt tcccggcaat
      721 atcagtcgtg ggcgacgggg tggataaatg gggtcagaga catttaaatg cccccttcaa
      781 tagatagtcg ttcatgcata atcgtctcca ccccatttgt cgggtcgcga aggccggaac
      841 aatcgacggc gcaatcataa gccagctatt aaggcttagt gttttccggt actgccgaaa
      901 ccgccctcgc cgcgcttgct ggcagggaat tcctccacca cgttgaaatc ggcatgcgtc
      961 accggcacga tcaccagctg cgcaatccgc tccagcgggt tgaggataaa cggcgtgcgg
     1021 ccacggttcc agcaggaaac gaacaactgc ccctggtaat ccgagtcgat cagcccgacc
     1081 aggttgccga gcacgatgcc gtgcttgtga cccagaccgg agcgcggcag gatcagtgcc
     1141 gcatagccgg ggtcggcgat gtggatggcc atgccggtcg ggatcagttc ggtctcgcct
     1201 ggatgcaagg tcagtacgtg gtcgatgcag gcgcgcaggt caagtccggc cgagccgggc
     1261 gtagcatagc tgggttgatg gtggtgcagc cgcgcatcca gcaacttgag atcgactttg
     1321 agcgtcatgg tttccttcct gtggcgaatt tgggttcatt cagcatggcg acaacgtgct
     1381 gcagcagctg ccgcgccaac actgacttgg gcgcccgcga caacggatgg gtgccgttgt
     1441 cgtcaagcag gataagttcg ctgtcatcgc caccgagcgc atcctggatc aggttggcgg
     1501 caagcaaggg cagatgcttg cgctgccgct tctgctcggc gaattcatag aggttttcgc
     1561 tttccgcggc gaaacccaca cagaagggtg gattgggcaa gccggccacg ctggcgagga
     1621 tgtccacggt cggcaccagt tccagggtaa ggttttgctc gtttttcttg atcttctgtt
     1681 cgctggggtt gggcgcgcaa taatcggcca ccgcggcaac gccgataaaa atatcctgtc
     1741 ccgccagatt gtccatgacc gccgccaaca tttgccgggc gctggtgacc tggacgagcc
     1801 tggcccccgt tggcactggc aggctggtct ttccgctcac caatgtgacc tccgcaccca
     1861 tttccagcgc ggcacgggca atcgcgtagc ccatcttgcc ggaactcaga ttggcgatgc
     1921 cgcgcaccgc gtcgatggcc tcgaaagtcg ggccggcggt gagcagcact ttcctgccga
     1981 caagcagttt gggcagcggg ccggtttcca gcgcatccag cagcgcctgc ggctccagca
     2041 tgcgccccag gccggtttcg ccacacgcct ggtcgccgct gtcaggcccc aggacggcaa
     2101 taccatcgtt gattaattgc gccacattgc gctgcgtagc cgggtttttc cacatctgta
     2161 cgttcatcgc cggcgccacc agcagcgggc attcgccgcg cgccagacac agggtggaga
     2221 gcaggtcgtc cgccgcaccg tgtaccagct tggcaatgaa gtcggcgctg gccggcgcga
     2281 tcaggatggc atcggcgttg cgtgccaact cgatgtgcgc catgccgttg ccgctgcggt
     2341 catcccacaa gtcggtgaat accggcttgc cggatagcgc ctgcatcgtg accggggtaa
     2401 tgaactggca ggcagcttgg gtcatcacca cttgcacctc gacaccctgc ttgaccagca
     2461 ggcgcaccag ctcggccgcc ttgtaagcgg caatgccgcc ggtgatgccg accagcagac
     2521 gtttaacggg agaatgcgcc ataattgtat aagttattca agccgttcaa ttctatacga
     2581 aagggcatga cgtggcgatt acagactggc cggaaggcga gcgaccgcgc gagaaacttc
     2641 tggcgcaagg cacgcaagtg ttgtccgacg ccgaactgct ggcgatcttc ctgcgcgtgg
     2701 gagtgaccgg caagagtgca gtcgatctgg cgcgcgatct gctcaccgaa tttggcagcc
     2761 ttaacggcat tttcggcgcc agtcgcgccg cgctcactgc agtcaacggc atgggcgaca
     2821 gcaaatatgc ccagctacag gccatcttcg aaatgagccg tcgcgcgctg gaagaacaga
     2881 tgcggcagcg cgatgca
//
LOCUS       DAOMFA010000175         2819 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_5183_length_2819_cov_4.692836, whole genome shotgun sequence.
ACCESSION   DAOMFA010000175 DAOMFA010000000
VERSION     DAOMFA010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 2819)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2819)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2819
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5183_length_2819_cov_4.692836"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..501
                     /locus_tag="PKW44_07910"
     CDS             <1..501
                     /locus_tag="PKW44_07910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR51546.1"
                     /translation="DGGFRRSGLKRYTGEFKGIHVAKKGDIVMAVTDMTQERRIVARA
                     ALVPTLDNEFGIFSMDLVRIEPKPGLPKAFLYSFLRYSSFADEVKQHANGANVLHLSP
                     DRITDYRFTAPPADLMSRLASFVTPIVEELDTLANKIDNLRRTRDLLLPRLLSGQIDV
                     EALPVP"
     gene            624..1157
                     /locus_tag="PKW44_07915"
     CDS             624..1157
                     /locus_tag="PKW44_07915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HQR51547.1"
                     /translation="MAKLPNRQPVLKPQDFYLLLALAAYGDQGTTYPELAAFAGLSMS
                     EVHGALKRAEAARLLFFEAKRPRIMMAAFKEFLFHGAKYVFPAVRGSMVAGVPTAHAA
                     APLNAHIAPSADPAPVWPSIEGSVRGIALIPLYPSAPAAALRSPALYENLALFDALRA
                     GNVRERALAQELFEKRL"
     gene            1154..1870
                     /locus_tag="PKW44_07920"
     CDS             1154..1870
                     /locus_tag="PKW44_07920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR51548.1"
                     /translation="MIAGDPNVQSVELVAQALGDLCDELVLVGGCAASLLIDAPTAPP
                     PRVTYDVDLLAVVSALSNYHALEDKFAQRGFRRDMSPDAPICRWRIGGIMVDLMPTDE
                     SVLGFSNRWYPYAAASAMQMTLPSGRNIRLVSAPAFLATKFEAFQTRGNSDFALSHDF
                     EDIINVIEGRAATVAEVAASASDLRTYLAAQFARISQREDLQNVLPGLVAYDELYAQR
                     AAAVTQRIAAIASLAGTGTA"
     gene            1867..>2819
                     /locus_tag="PKW44_07925"
     CDS             1867..>2819
                     /locus_tag="PKW44_07925"
                     /inference="COORDINATES: protein motif:HMM:NF016220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease"
                     /protein_id="HQR51549.1"
                     /translation="MSTHAYSEDQLVEQPAIGLFATLGWQTVSAMEETFGKGGTLGRE
                     TRGEVVLAERLRAALGKFNPTMPPEAISNAVDELTRDRSAMSLEAANREVYQLLKEGI
                     AVSVPDRDALTPGPSPRGRGGQTTERLRVVDWETPANNDFLLVSQFSVTGSLYTCRPD
                     LVGFVNGLPWVVIELKKPGVPARAAFDENLTHYKQQIPALFWSNALLIASNGTDSRVG
                     SLTADWERWVEWKRIEREDEPRRVSLEVMLRGTCDPARLLDLVENFTLFSEHKAGVVK
                     IIGQNHQFLGVNNAIASMLEARTLGHGRGGVFWQTQGSGKS"
BASE COUNT          528 a          821 c          882 g          588 t
ORIGIN      
        1 gacggcggtt ttcggcgctc tggcctgaag cggtataccg gagagttcaa gggcatccac
       61 gtcgctaaaa aaggtgacat cgttatggca gtcaccgaca tgacgcaaga gcggcgcatc
      121 gttgcgcggg cggcgttggt gccaaccttg gacaacgagt ttgggatctt ctcgatggat
      181 ctggtgcgca ttgaaccgaa gccggggttg cccaaggcat tcctatattc attccttcgg
      241 tattcctcgt tcgccgatga ggtaaagcaa cacgccaatg gtgcaaacgt tctgcatctt
      301 tcgcccgatc gaatcaccga ctatcggttc accgccccgc ccgcggactt gatgagccgc
      361 ctcgcaagct tcgtcactcc catcgtggag gaacttgata cgctggcaaa caagatcgac
      421 aacctccgcc gcacccgcga cttgctgctg ccacgtctgc tctccggcca gattgacgtg
      481 gaagcattac cggttccctg atgaatacgt ttgttttcgc cattcgcgaa tcgcgaaacc
      541 gcttggccgg tagcgcgaaa actgtttgac agtgcattgt ttattatgta ctttcgtatt
      601 tcgcgaaaca cgaaagaagt gtgatggcaa aactccctaa ccgtcagccg gtgctcaagc
      661 cgcaggattt ttatctgctg ctggccttgg ctgcgtatgg cgatcaaggc acgacttacc
      721 ccgagctggc ggcgtttgcg gggttgagca tgtcagaagt gcatggtgcg ctgaagcgcg
      781 cggaggcggc acgtttgctg ttttttgagg cgaagcggcc acgcattatg atggctgcct
      841 tcaaggaatt tctgtttcat ggcgccaagt atgttttccc tgccgtgcgt ggcagcatgg
      901 tggccggggt gccgacggca catgccgccg caccgttgaa tgcgcatatc gcgccctcgg
      961 cagacccggc gccggtgtgg ccgtcgatcg agggttcggt gcgcggcatc gccttgattc
     1021 cgctgtatcc ctccgccccc gctgcggcat tgcgcagccc ggcgctatat gaaaaccttg
     1081 ccctgttcga tgccctgcgc gctggcaatg tgcgagaacg ggcgctggcg caagaactgt
     1141 tcgagaagcg cctgtgatag cgggtgatcc gaatgtgcag agtgtggagc tggtggccca
     1201 ggccctgggc gacctgtgcg atgaactggt tctggtgggc gggtgtgcgg ccagtctgct
     1261 gatcgacgcc ccgaccgcgc cgccgccgcg tgtgacttac gatgtcgatt tgctggcggt
     1321 ggtgagtgcc ttgagcaatt accatgcgct ggaagacaaa tttgcgcagc gtggattcag
     1381 gcgtgatatg tcgcctgatg cgccgatctg tcgttggcga atcggcggga tcatggtgga
     1441 tttgatgccc accgatgaat cggtacttgg cttttccaat cgctggtatc cctacgcggc
     1501 agcgtcggcc atgcagatga ccctgcccag cggcagaaat atccgcctgg tctcggctcc
     1561 tgcctttctc gccaccaagt tcgaagcgtt tcagacgcgc ggcaattctg attttgcgct
     1621 cagccatgat ttcgaagaca tcatcaatgt gattgaggga cgggcggcga cagtggcgga
     1681 ggttgctgca agcgcttcgg acttgcgcac ttatctggct gcgcagtttg cccggataag
     1741 ccagcgcgag gatttgcaga atgtgctgcc aggcctggtt gcttacgatg agctttatgc
     1801 gcagcgggca gcagcggtga cacaaagaat tgcggccatc gccagcctgg ccggcacggg
     1861 aacggcatga gcacccacgc ttactccgaa gaccagctcg tcgagcagcc cgccatcggg
     1921 ctgtttgcca cgcttggctg gcaaacggtg tcggcgatgg aagaaacttt cggcaagggt
     1981 ggaacccttg gccgcgaaac cagaggtgag gtggtgctgg cggagcgcct gcgtgcggca
     2041 ctcggcaaat tcaacccaac aatgccgccg gaagccatca gtaacgccgt tgatgaactg
     2101 acgcgcgacc gctcggcgat gagtctggag gccgccaacc gtgaagttta tcagctgctc
     2161 aaggaaggca tcgcggtatc cgttcccgac cgcgatgccc tcacccccgg cccctctccc
     2221 agagggcgag gggggcaaac caccgaacgc ctgcgcgtgg tggattggga aactccggcc
     2281 aacaacgatt tcctgctggt cagccagttc agcgtgaccg gatctctcta cacctgccgg
     2341 cccgatctgg tcggcttcgt caatggcctg ccctgggtgg tgatcgaact caagaaaccc
     2401 ggcgtgccgg cgcgcgctgc attcgatgaa aacctcaccc actacaagca gcagattccg
     2461 gcgctgttct ggagcaatgc tttgctcatt gccagcaacg gcaccgatag ccgtgtcggc
     2521 tcgctcaccg ccgactggga acgatgggtg gagtggaagc gcatcgagcg cgaggatgag
     2581 ccgcgccgcg tgtcgctgga ggtgatgctg cgcggcacct gcgaccccgc ccgcctgctc
     2641 gatctggtgg agaacttcac gctgttttcc gagcacaagg ccggggtggt caagatcatt
     2701 ggccagaacc accagttcct cggcgtcaat aatgccatcg cctcgatgct tgaagcgcgc
     2761 acgctgggcc acgggcgggg cggcgtgttc tggcagacgc agggcagtgg caagagctt
//
LOCUS       DAOMFA010000176         2806 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_5222_length_2806_cov_4.487459, whole genome shotgun sequence.
ACCESSION   DAOMFA010000176 DAOMFA010000000
VERSION     DAOMFA010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 2806)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2806)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2806
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5222_length_2806_cov_4.487459"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..410)
                     /locus_tag="PKW44_07930"
     CDS             complement(<1..410)
                     /locus_tag="PKW44_07930"
                     /inference="COORDINATES: protein motif:HMM:TIGR02098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HQR51550.1"
                     /translation="MSLVATCSACGTRFRVRPEQLAAHRGDVRCGKCSHVFNALENLS
                     ETASSASPSTIVVPETPASEIIAEETWVTAIDTAALPESPAADSEEAAPPESIKTLEK
                     LPLVEEPDPLQEYKFVIEPEPEPEPEPEPEPEPEP"
     gene            complement(407..1294)
                     /gene="prmA"
                     /locus_tag="PKW44_07935"
     CDS             complement(407..1294)
                     /gene="prmA"
                     /locus_tag="PKW44_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013440847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HQR51551.1"
                     /translation="MSWQLLKIEAGEESAEILSDALMELGALSVSIEDADAATDDELP
                     IFGEPGEAPPGMWHHNVVSALLPANTAADTLLRELEQSTGLGPLNYAVDTIAEQDWVR
                     ATQSQFEPIRVRDNLWIVPSWHAAPNPAALNIVLDPGLAFGTGSHPTTHLCLAWLADH
                     LHAGESVLDYGCGSGILAIAAKRLGADNVLGTDIDPQAMEASHYNADNNRAEVRFVLP
                     DKMPAFVADVVVANILASPLKVLAPALAGYCRHGGRIVLSGILREQAEDVSATYAAWF
                     DMHAPVVMENWVLLTGTKR"
     gene            complement(1294..2643)
                     /gene="accC"
                     /locus_tag="PKW44_07940"
     CDS             complement(1294..2643)
                     /gene="accC"
                     /locus_tag="PKW44_07940"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013440848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HQR51552.1"
                     /translation="MFEKVLIANRGEIALRIQRACRELGIKTVAVHSEADREAKYVKL
                     ADESVCIGPAPSSQSYLHVPAVISAAEVTDAEAIHPGYGFLSENADFAERVEQSGFVF
                     IGPRPETIRLMGDKVSAKDAMKKAGVPCVPGSDGALGDDADEILRMAKQIGYPVIIKA
                     AGGGGGRGMRVVHTEAALLNAVSMTKAEAQAAFGNPMVYMEKFLTQPRHIEIQVLADE
                     HGNAVYLGERDCSMQRRHQKVLEEAPAPGVDRKLIATIGERCAEACRTIGYRGAGTFE
                     FLYENDEFYFIEMNTRVQVEHPVTELITGIDIVQTQLRVAAGEILPFKQKDIQLRGHA
                     VECRINAEDPHNFVPSPGRITTFHIPGGPGIRVDTHAYQGYMVPPHYDSMIGKLLAYG
                     DTREQALNRMRIALSEMVVEGIKTNIPLHLELMNDGEFRQGGTSIHYLEHKLGITSK"
     gene            complement(2659..>2806)
                     /locus_tag="PKW44_07945"
     CDS             complement(2659..>2806)
                     /locus_tag="PKW44_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HQR51553.1"
                     /translation="VNVGDTLCIVEAMKLLNEIEADKAGVVKAILVESGQPVEYGEPL
                     FIIG"
BASE COUNT          481 a          809 c          911 g          605 t
ORIGIN      
        1 ggctctggct ctggctctgg ctctggctct ggctctggct ctggctctgg ctctatgacg
       61 aacttatatt cctgcagcgg gtcgggttct tccaccaaag gcagcttttc aagcgtcttt
      121 atgctctcgg ggggcgcggc ttcttcggaa tcagcagccg ggctttccgg caatgcggcg
      181 gtgtcgattg ctgtgaccca ggtttcctct gcaatgattt cactagcagg tgtttcaggc
      241 accacaatgg ttgaaggcga agcgctagaa gcagtttcgg acaggttttc caatgcgttg
      301 aatacgtggc tgcacttgcc gcaacgcacg tcgccgcgat gcgcggccaa ttgttccggt
      361 cgcacccgga aacgggtgcc gcatgccgag caggtggcga ccaggctcat cgtttggttc
      421 ccgtcagcag tacccagttt tccatgacga ccggggcatg catgtcgaac caggcggcgt
      481 aggtggcgct gacgtcctcg gcctgttcgc gcaggatgcc ggacaagaca atgcgtccgc
      541 cgtgacggca atagccggcc aaggcaggtg ccagcacctt gagcgggctg gcgaggatgt
      601 tggcgaccac tacgtcggcc acaaacgccg gcattttatc cggcaataca aagcgcacct
      661 cggcgcggtt attgtcggca ttgtagtggc tggcttccat ggcctgtggg tcgatgtcgg
      721 tcccgagtac attgtctgcg cccagccgct tggcggcgat ggccaggatg ccggagccgc
      781 agccgtaatc gagcacggac tctccggcgt gcaagtgatc ggccagccag gcgagacaaa
      841 ggtgggtagt ggggtggctg ccggtgccga aggcgaggcc agggtcgagc acgatgttga
      901 gggcggctgg attgggcgcg gcgtgccagg aaggcacgat ccacaggttg tctctaacgc
      961 ggatgggttc gaactgcgac tgggtggcgc gcacccagtc ctgttcggca atggtgtcca
     1021 ccgcgtaatt cagcggcccc agtccggtgg actgctccag ttcgcggagc agggtatccg
     1081 cggcagtatt ggcggggagc agggcgctga cgacgttgtg gtgccacatg ccaggtggcg
     1141 cctcgcccgg ctcgccgaag atgggcagtt cgtcgtcggt agcggcatcg gcatcctcga
     1201 tgctgacgga aagcgcgccc agttccatca gggcatcgct caaaatttcg gcggactctt
     1261 ccccggcttc gattttgagc aattgccagg acattatttg ctggtgatgc ccagcttgtg
     1321 ttccagataa tggatgctgg tgccgccctg gcggaactcg ccgtcgttca tcagttcgag
     1381 atgtagcggg atgttggtct tgatcccttc caccaccatt tccgacagcg cgatgcgcat
     1441 gcggttgagc gcctgctcgc gggtgtcgcc gtaagccagc agcttgccga tcatggagtc
     1501 gtagtggggc ggcaccatat agccctgata ggcatgggta tcgacgcgga tgccaggccc
     1561 gcccgggata tggaaagtgg tgatgcggcc tggcgaaggc acgaagttgt gcggatcctc
     1621 ggcgttgatg cggcattcca cggcatggcc gcgcagttgg atgtccttct gcttgaacgg
     1681 cagtatttcg ccggcggcaa cgcgcaactg cgtttgtacg atgtcgatgc cggtgataag
     1741 ttcggtgacc ggatgttcca cctgtacccg agtgttcatc tcgatgaaat agaattcgtc
     1801 gttttcgtag aggaactcga aggtgccggc accacggtat cctatggtcc ggcaagcttc
     1861 ggcgcagcgt tcgccgatgg tggcgatcag cttgcgatcc acacccggcg ccggggcttc
     1921 ttccagcact ttttgatggc gacgctgcat ggaacagtcg cgttcgccca gatagacggc
     1981 gttgccatgc tcgtcggcca gtacctgtat ttcgatatgg cgcggctggg taaggaattt
     2041 ttccatgtac accatgggat tgccgaaggc ggcctgcgcc tcggccttgg tcatgctgac
     2101 tgcattgagc agcgcagcct cggtatgcac cacgcgcatg ccgcgtccgc cgccgccgcc
     2161 ggcggccttg atgataaccg ggtagccgat ctgtttggcc atgcgcagga tttcatcggc
     2221 atcatccccc aaagcaccgt ccgatccggg cacgcagggc acgccggctt tcttcatggc
     2281 gtccttggcg ctgaccttgt cgcccatcag gcgaatggtt tccgggcgtg ggccgatgaa
     2341 cacgaagccg gactgttcca cgcgctcggc gaagtcggcg ttttccgaca ggaagccgta
     2401 gccgggatgg atggcttcgg catcggtcac ctcggccgcg ctgatcacgg ccgggacatg
     2461 cagatagctc tggctggaag gtgccgggcc gatgcagacc gattcgtcgg ccaatttgac
     2521 gtacttggct tcgcgatccg cctcggagtg cacagcgacg gtcttgatac ccagttcacg
     2581 gcaggcgcgc tggattcgca aagcaatctc gccacgattg gcgatgagga ctttctcgaa
     2641 catggggaac ccttttattt agccgatgat gaacagcggt tcgccgtatt cgaccggctg
     2701 gccgctctcg accaggatcg ccttgaccac gccggccttg tcggcttcga tttcgttgag
     2761 cagtttcatg gcttcgacga tgcacagggt atcgccgaca ttgacg
//
LOCUS       DAOMFA010000177         2773 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_5332_length_2773_cov_4.460265, whole genome shotgun sequence.
ACCESSION   DAOMFA010000177 DAOMFA010000000
VERSION     DAOMFA010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 2773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2773
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5332_length_2773_cov_4.460265"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            181..975
                     /locus_tag="PKW44_07950"
     CDS             181..975
                     /locus_tag="PKW44_07950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR51554.1"
                     /translation="MELLDSQWFLRMVAAGGKTPAERLLAVYAITRTWIAAPGIRELF
                     AKAYPANKAALHSCAGLKTHLTEIATAAKVQNPSILASQLVLLLQGAIAEELRNPGAG
                     ALEEAENAARAVIASACNIQKRQRLRMRAMGGLTAASILVAIVSLYPHDGRMFSPAVA
                     ATAAPLAPAEVAAVPEGVSPDEMEQVLLLQRQIEDGRCPAPQLLALPQGQVTAYMNAI
                     NLRTPENPAADRENMRAFLAWFRQTRSTECYFPPSNGHTTVAWVKR"
     gene            979..1536
                     /locus_tag="PKW44_07955"
     CDS             979..1536
                     /locus_tag="PKW44_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HQR51555.1"
                     /translation="MATQMREYILGVASDLFSSQGINATSVDTIVAKADIAKVTLYKY
                     FKSKEQLILEYLREYDDRLWKKLSEVTAQQGDAASKLTALVNAMLDWIADPDFKGFAF
                     INASVEFPQSESPVHQSSLEFAQTLRNTLATLAKEAGIKGADTLALQLSLVIEGAAIT
                     ERTQRGSGAAEHAKALAKVLIGSAK"
     gene            1677..2702
                     /locus_tag="PKW44_07960"
     CDS             1677..2702
                     /locus_tag="PKW44_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HQR51556.1"
                     /translation="MGTATASQLADWRAHALQLESEINKVVVGQERPVRLITTAIFSR
                     GHVLLEGDVGVGKTTLLRAFTRGIGGGYERVEGTIDLMPNDLVYHTYLGEDGRPHVDA
                     GPLLREGENLAVFFFNEINRARPQVHSLLLRVMAERTLSAFNKEHHFPHMLVFADRNQ
                     VEKEETFEIPSAARDRFMMEIAIAVPSDRDIMESLIFDTRYHNADQLVQNVQADVLHF
                     DKLNDIAVALQDHVQASPTLRKYALELWLATKSPARYGVRIEGVDLNRLMLAGASPRG
                     MSMMLRAARVNAWLNGRDAVLPEDIHAVFHETVAHRLVFSPIYEMRRTEIARELLNGI
                     VHAIAAP"
BASE COUNT          581 a          893 c          827 g          472 t
ORIGIN      
        1 ccgtctttca cgagaacaat ttatgggttc agcatcaata acatatgggg tcagcatcaa
       61 ttagcttttt gtgcccaagc aaatgccgcg tagcccttac tttgctataa gcaaattttc
      121 tggcaaattc ggcggcgctg tggaacaatg tactaactag tatgtttaca ggggcgcaag
      181 ttggaactgc tcgatagcca gtggtttttg cggatggtcg ccgccggggg taaaacccct
      241 gcggaaaggc tgctggccgt ctacgccatc accaggacct ggatcgccgc gcccggcatc
      301 cgcgagcttt tcgccaaggc ctacccggcc aacaaggccg ccctgcattc ctgcgccgga
      361 ctgaagacgc acctcaccga aatcgctacg gctgccaagg tgcagaaccc ttccatcctg
      421 gccagccagc tggtgctgct gctgcaaggc gccatcgccg aggagttgcg caaccccggc
      481 gccggcgcgc tggaggaggc ggaaaacgcg gcgcgggcag tcatcgccag cgcctgcaat
      541 atccaaaaaa ggcaacgcct gcgcatgcgc gccatgggcg gcctgaccgc ggcttccatc
      601 ctggtggcaa tcgtcagcct gtacccccat gacggcagga tgttctctcc cgcggttgcc
      661 gccacggcgg cgccactcgc gccggcggaa gtcgccgccg tgccggaagg cgtcagcccg
      721 gacgaaatgg aacaggtgct gctgctgcag cgccagatcg aggacggccg gtgcccggcc
      781 ccgcaattgc tggcactgcc gcaaggccag gtgacggcct acatgaacgc catcaacctg
      841 cgcaccccgg aaaaccccgc cgcagaccgg gagaacatgc gggccttcct ggcgtggttc
      901 cggcagacgc gctccaccga atgctatttc cccccttcca atgggcatac caccgtcgcg
      961 tgggtaaaga ggtaaaccat ggccacacaa atgcgtgaat acatcctggg cgtcgcctcg
     1021 gacctgtttt ccagccaggg catcaacgcc accagcgtgg ataccatcgt cgccaaggcc
     1081 gatatcgcca aggtgacgct gtacaagtat ttcaaatcca aggagcagct gatcctggaa
     1141 tacctgcgcg aatacgacga ccgcctgtgg aagaagcttt ccgaggtgac cgcccagcag
     1201 ggcgacgccg cttccaagct gaccgcgctg gtcaacgcca tgctggactg gattgccgac
     1261 ccggatttca agggcttcgc cttcatcaac gctagcgtcg agtttccgca gtccgaaagc
     1321 ccggtgcacc agagctccct ggaattcgcc cagacgttgc gcaacacgct ggccacgctg
     1381 gcgaaggaag caggaatcaa gggtgccgac accctggcct tgcaattgtc gttggtgatc
     1441 gagggcgccg ccatcacgga acgcacgcag cgcggctcgg gcgcggcgga gcatgcaaag
     1501 gcgctggcga aagtgctgat cggatcggca aaataatact tactagttag tatgttttat
     1561 gccatactgc cgacaagggt tgtttcgggg cgggtgctcc cttaaactcc ccgaagctcg
     1621 ccatcaggca ctaccaccgc aggacaaaca acaggcactt gtcacaaagg gagatcatgg
     1681 gaaccgcaac cgctagccaa ctggccgact ggcgcgcaca cgcgctgcaa ctggaatccg
     1741 aaatcaacaa ggtcgtggtc gggcaggaaa ggccggtgcg cctcatcacc accgccatct
     1801 tctcccgcgg ccacgtgctg ctggaaggcg acgtgggggt gggcaagacc accctgctgc
     1861 gcgccttcac acgcggcatc ggcggcggct acgagcgggt ggaaggcacc atcgacctga
     1921 tgcccaacga cctcgtgtac cacacctatc tgggcgagga tggccgcccg cacgtggatg
     1981 ccggcccgct gctgcgcgag ggcgaaaacc tggcggtgtt cttcttcaac gaaatcaacc
     2041 gcgcccggcc gcaggtgcac tcgctgctgc tgcgcgtgat ggcggaacgc accctgtccg
     2101 ccttcaacaa ggagcaccac ttcccgcaca tgctggtgtt cgccgaccgc aaccaggtgg
     2161 aaaaggaaga aaccttcgaa atcccctccg ccgcgcgcga ccgcttcatg atggaaatcg
     2221 ccattgcggt gccgagcgac cgtgacatca tggaatcgct gattttcgac acgcgctacc
     2281 acaatgccga ccagctggtg cagaacgtcc aggcggatgt tttacatttc gacaagctga
     2341 acgatatcgc cgtcgcactg caggatcacg tgcaggccag cccgacgctg cgcaaatacg
     2401 cgctggaact ctggctggcg accaagtcgc cagccagata cggcgtcagg atcgaaggcg
     2461 tcgacctcaa ccggctgatg ctggccggcg ccagcccgcg cggcatgtcg atgatgctgc
     2521 gtgcggcgcg ggtcaacgcc tggcttaacg ggcgcgacgc cgtgctgccg gaagacatcc
     2581 atgccgtgtt ccacgagacg gtggcgcacc ggctggtatt cagcccgatc tacgaaatgc
     2641 ggcgtacgga aatcgcgcgt gaactgctga acggtatcgt ccacgccatc gctgcaccct
     2701 gagagataaa atcatgacgc ctgatcgggc caaagagttt tcctaccaga tcggctggcg
     2761 ctcgcgcgcg cgc
//
LOCUS       DAOMFA010000178         2708 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_5544_length_2708_cov_4.747079, whole genome shotgun sequence.
ACCESSION   DAOMFA010000178 DAOMFA010000000
VERSION     DAOMFA010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 2708)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2708)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2708
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5544_length_2708_cov_4.747079"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1758
                     /locus_tag="PKW44_07965"
     CDS             <1..1758
                     /locus_tag="PKW44_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HQR51557.1"
                     /translation="VLVVVDEEHEPAVRRGLGGDPGLSISAAPATRHGARRRWLQRVA
                     GSGEKSELSWRDGEVLRLPSRIDLFAEKSLNRDLYWWLVALSAIDVEPGLPWIVRNQQ
                     ATMCILKRFPGLSVRYERLVHALIQTRMPVGNLPADEAAQEKAIRQALQQPGSVSSLP
                     TASCPFQPVPLWLHPQPPVSRSDAAAGNDGAPESGGGARTEKGRRKYLAGRTDTPEDK
                     NGFMLMFRAESIFSWAEFVKVNRPQDDEDDAKSARLAAEDMDSLSIARDGKTNAASVR
                     FDLDLPPAAADAVPLGEGVALPEWNWKKQLLQPDYCRLQEMVAADATPLELPLALRRT
                     AARLRRQFQALAPTRHWLRAQPDGEEADLDAWVQLAADRASGMCIGERGLYRAQVNQQ
                     RDLACLLLADLSLSTDAYVSDHVRAIDVIRDSLLLFSEALTATGDRFALYGFSSLRRS
                     HVRFHHLKRFDDSYDGEVRGRIVNIKPGYYTRMGAAIRHASSLLARQRSRQRLLLLLT
                     DGKPNDIDQYEGRYGIEDTRMALMEARRQGLRPFCVTIDSESGDYLPHLFGAGGYVVI
                     RKPEDLPKELPLLYAQMTR"
     gene            complement(1850..2368)
                     /locus_tag="PKW44_07970"
     CDS             complement(1850..2368)
                     /locus_tag="PKW44_07970"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HQR51558.1"
                     /translation="MSIPAQDGNSPHVLGDLIADIEQSHHAFTRNALAHIGKLFEDNA
                     DGSVWNSPLLQVCFDELQADLIPHLMKEERILFPYVIALENDPAHLPHSCFGSVANPI
                     HMMKIEHDRVKVLLAKLRALTSNYAVSANAKTNALYMALSALDRDLEQHIKTEDEVLF
                     PRALQLERASTA"
     gene            complement(2460..>2708)
                     /locus_tag="PKW44_07975"
     CDS             complement(2460..>2708)
                     /locus_tag="PKW44_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HQR51559.1"
                     /translation="VALLTCSQRLIGYLLQIADASGNSNRITLPTSKTNIASLLNLTP
                     ETLSRTMLKLHQSRLIEVHGKEISIINVAGLREFEYGI"
BASE COUNT          518 a          754 c          859 g          577 t
ORIGIN      
        1 gtcctcgtcg tcgtcgacga ggagcacgag cccgcggttc ggcgcggcct gggcggcgat
       61 cccggcctga gtatttcggc cgcacctgcc acccggcatg gtgcgcggcg gcgctggttg
      121 cagcgcgtgg ccggcagtgg agaaaaaagc gagttgtcgt ggcgcgatgg cgaggtcttg
      181 cgtcttccgt cacgcatcga cctgtttgcc gaaaaatccc ttaaccgtga tctttattgg
      241 tggctggtcg ccctgtcggc gattgatgtg gagcctggct tgccgtggat tgtccgcaac
      301 cagcaggcga ctatgtgcat actcaagcgt tttcccggat tgtcggtgcg ctatgaacgg
      361 ctggtgcatg ccctgatcca gacgcgaatg ccggtcggca atctgcctgc cgatgaggcc
      421 gcgcaagaga aggccatccg acaggctttg cagcagcccg gcagcgtgtc gagcttgccg
      481 acggccagct gtccattcca gcccgtgccg ttatggctac atccgcagcc gccggtcagc
      541 cgatcagatg ctgcggccgg taacgatggt gcgccggagt ccggcggcgg tgcccgcact
      601 gaaaaaggcc gccgcaagta cctggcaggg cgtaccgata caccggaaga caagaacggc
      661 ttcatgttga tgttccgtgc cgagagcatt ttttcctggg ctgagttcgt caaggtcaac
      721 cgcccacagg atgacgagga cgacgccaaa tcggccaggc tggcggccga ggatatggat
      781 agcctcagca ttgcgcgcga tggcaagacc aatgccgcca gtgtgcgttt tgacctggat
      841 ttgcctcccg cagcagcgga tgccgtgccg ctgggggagg gcgttgcact gcccgaatgg
      901 aattggaaaa aacagctgtt gcagccggat tactgccgct tgcaggaaat ggtagcggcc
      961 gatgcgaccc cactggaact gccgctggcg ctacgccgca cagccgcccg cctgcgtcga
     1021 cagttccagg cgttggcacc cacccggcac tggctcaggg cgcagcccga cggagaagaa
     1081 gccgacctcg atgcctgggt gcaactagcg gccgaccgtg ccagcggcat gtgtatcggg
     1141 gagcgcggtt tgtaccgggc gcaggttaat cagcagcgcg atctggcctg cctgctgctg
     1201 gctgatcttt cgttgtcgac cgacgcctac gtctccgatc acgtccgggc gatcgacgtc
     1261 atccgtgata gcctgctgct gttttccgaa gcgctcacgg ccaccggtga ccgtttcgcg
     1321 ctctacggct tttcatcgtt gcgtcgcagc cacgtgcgct ttcatcatct caagcgcttc
     1381 gacgacagtt acgatggcga ggtgcgcggg cgtattgtga acatcaagcc gggctattac
     1441 acccgcatgg gcgcggcgat ccgtcacgcc agtagcctgc tggccaggca aagaagccgc
     1501 cagcggctat tgctgctgct taccgacggc aagcccaacg atatcgatca atacgaaggc
     1561 cgctatggca tcgaggatac gcgcatggcg ttaatggaag cccgacggca ggggttgagg
     1621 ccattctgcg tgacgataga cagcgaatcc ggcgattatt tgccccacct gttcggtgcg
     1681 ggcggctatg tggtgatccg caagccggaa gacttgccga aggagttgcc gctactctat
     1741 gcgcagatga cgcgctaacg ccgtgtatta ccgggtcacg cccaacctca agtccgtcgt
     1801 ctggcacaga attcgggttg aaatggtttt ttgggcgtgg cgtagggcgt caggccgtag
     1861 acgcacgttc cagttgcaac gcgcgcggga acaggacttc gtcttccgtt ttgatatgct
     1921 gttccaggtc ccggtccagt gcggataagg ccatgtataa ggcattggtt tttgcattgg
     1981 ccgatacggc gtaattcgag gttagtgcgc gtagtttggc cagcaggacc ttgacgcggt
     2041 catgttcgat tttcatcatg tggatagggt tggcgacact gccgaaacag gaatgcggca
     2101 gatgtgccgg gtcgttttca agcgcaatga cgtagggaaa gagaatgcgc tcctccttca
     2161 tcaggtgcgg gatcaggtca gcctgcagtt cgtcgaaaca gacctgtagt aaggggctgt
     2221 tccatactga cccatccgcg ttgtcttcga acagtttccc gatatgtgcc agagcattgc
     2281 gtgtaaaggc gtgatgcgat tgttcgatat cggcgatcaa gtcgccgagc acatggggcg
     2341 agtttccatc ctgcgccggt atggacatcg acatacttcc tgaccgaatt tagatcctgt
     2401 caagcatatc gatcaggcgg tatttttcct tgattcaggt caaagaccgt gtcgaaaggt
     2461 cagattccgt actcgaactc ccgcaggccg gcaacattga tgatggaaat ttccttgcca
     2521 tggacctcga tgagacgcga ctgatgcagt ttcagcatgg tgcgtgagag cgtttccggg
     2581 gtgaggttga gcagtgatgc aatattcgtt ttgctggtgg gtagggtgat ccgattcgaa
     2641 ttgccggagg cgtcggcaat ctgcagcaga tagccgatca gacgctggct acatgtgagc
     2701 agggcgac
//
LOCUS       DAOMFA010000179         2697 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_5591_length_2697_cov_4.427706, whole genome shotgun sequence.
ACCESSION   DAOMFA010000179 DAOMFA010000000
VERSION     DAOMFA010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 2697)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2697)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2697
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5591_length_2697_cov_4.427706"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(35..1126)
                     /gene="rfbB"
                     /locus_tag="PKW44_07980"
     CDS             complement(35..1126)
                     /gene="rfbB"
                     /locus_tag="PKW44_07980"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HQR51560.1"
                     /translation="MSILVTGGAGFIGSNFVLDWLALNDEPVVNLDKLTYAGNLQNLA
                     SLQGNARHVFVQGDIGDGTLVGRLLAEHKVRAVVNFAAESHVDRSIHGPEDFVQTNIV
                     GTFRLLEAVRAYWQNLPSPSGRGGGGEGVSQADFLFLHIAAYQVYGSLTTYDPPFSEE
                     NRYEPNSPYSASKAASDHLVRAYHHTYGLPVLTTNCSNNYGPYHFPEKLIPLCIHNAL
                     AGKPLPIYGDGQQIRDWLYVKDHCSAIRRVLEAGQISEVYNVGGWNEKPNLDVVRTLC
                     AILDEEQPRADGKSYAEQITYVTDRPGHDRRYAIDARKLERELGWKPQETFETGIRKT
                     VRWYLDHQGWVANVTSGAYRNWVERQYSA"
     gene            complement(1247..2551)
                     /locus_tag="PKW44_07985"
     CDS             complement(1247..2551)
                     /locus_tag="PKW44_07985"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HQR51561.1"
                     /translation="MKTGLIQRLNEKQATIGIVGLGYVGLPLVLRYCEVGYKVIGFDI
                     DPAKVDALRQGRSYIERIPDAAIRQTLDQGFEPTTDFSCAAEADALILCVPTPLNKYR
                     EPDLSFVLDTTDSLTPYMRPGQVLSLESTTYPGTTDEELLPRIEARGFKVGQDAFLVY
                     SPEREDPGNATYSTRSIPKVCGGYSPDCLEVGMALYGQVIDQVVPVSSTRAAELTKLL
                     ENIHRAVNIGLVNEMKVVADKMGIDIHEVIRAAATKPFGFVPYYPGPGLGGHCIPIDP
                     FYLTWKAREYGVHTRFIELAGEVNASMPDYVVSKVLTALNSARKSVNGSRILVLGIAY
                     KKNVDDMRESPSVILMEKLRDLGAEVAYSDPHIPVFPKLREHAFDLASVALTPENLSV
                     FDCVLIATDHDKFDYALVRRHAPLIVDSRGRYPEAAANIVKA"
BASE COUNT          478 a          780 c          892 g          547 t
ORIGIN      
        1 ccgacctgac cgtttttacc gagcagcagg atgttcacgc gctgtactgc ctttccaccc
       61 aattgcggta cgcgccgctg gtgacgttgg cgacccagcc ctggtggtcg aggtaccagc
      121 gcacggtctt gcggatgccc gtttcaaaag tttcctgcgg cttccagccg agctcgcgct
      181 ccagcttgcg ggcgtcgatg gcatagcggc ggtcgtgccc cgggcggtcc gtgacatagg
      241 tgatttgttc ggcgtaggac ttgccgtcgg cgcgcggctg ctcctcgtcc aggatggcgc
      301 agagggttct cacgacgtcg aggttgggct tttcgttcca gccgccgacg ttgtagacct
      361 cgctgatttg tcctgcctcc agcacgcggc ggatcgcgct gcagtgatcc ttgacgtaga
      421 gccagtcgcg gatctgctgg ccatcgccgt agatcggcag cggcttgccg gccagcgcgt
      481 tgtggatgca cagcggaatg agtttttccg ggaagtggta gggcccgtaa ttgttggagc
      541 agttggtggt gagcaccggc aggccatagg tgtggtggta ggcgcgtacc agatggtcgg
      601 aagccgcctt gctggccgag taggggctgt tgggctcata gcggttttcc tcggagaacg
      661 gcgggtcgta tgtggtgagc gaaccgtaga cctggtaggc ggcaatgtgc aggaagagga
      721 agtccgcctg gctgacgccc tcacccccgc cccctctccc agagggcgag gggagatttt
      781 gccagtatgc acgcacggcc tcgagcaggc ggaaggtgcc gacgatattg gtctggacga
      841 aatcctcggg cccgtggatg gagcggtcga catggctttc ggcagcgaaa ttgaccacgg
      901 cccgcacctt gtgctccgcc agcagcctgc ccaccagcgt gccgtcgccg atgtccccct
      961 gcacgaacac atggcgcgca ttgccctgga gcgaggcaag gttctgcagg ttgccggcgt
     1021 acgtcagctt gtcgagattg acgaccggtt catcgttcaa tgccagccag tccagtacga
     1081 aattgctgcc gataaagccg gcgccgccgg taacgagtat agacacgtcg tagacccctc
     1141 ctgaatattt ttaggccgtg atggcggaac cgcgttaaag aaccgaccaa ttataccgga
     1201 aacgtctggc tggattaagc cgcagttcct gaagctggaa accgctctat gccttgacaa
     1261 tatttgcggc agcctcaggg tagcggccgc gcgagtcgac gatcagcggg gcatgccgcc
     1321 tgaccagcgc gtagtcgaat ttatcatggt cggtagcgat cagcacgcag tcgaaaacgg
     1381 acaggttttc cggcgtcagt gccacgctgg caaggtcgaa tgcatgctcg cggagcttgg
     1441 gaaagaccgg gatatgcggg tcgctgtagg caacttcggc gcccagatcg cgcaattttt
     1501 ccatcaggat gacggaaggg gactcgcgca tgtcgtccac gttttttttg taggcgatgc
     1561 caagcaccag gatgcggctg ccgttgacgg atttcctggc ggaattcagc gccgtcagga
     1621 ccttggaaac cacgtagtcc ggcatcgagg cgttgacttc gccggccagt tcgatgaaac
     1681 gggtgtgcac gccgtattcc cgcgccttcc aggtcaagta gaacgggtcg atgggaatgc
     1741 aatggccacc caagcccggt cccgggtaat agggcacgaa gccaaacggc ttggtggcgg
     1801 cggcgcggat gacttcgtgg atgtcgatcc ccatcttgtc ggcgacgacc ttcatttcgt
     1861 tgacgaggcc gatgttcacc gcacggtgga tgttctcgag cagcttggtc agttccgccg
     1921 cacgcgtgga actgaccggc actacttggt cgatgacctg gccgtacagc gccatgccga
     1981 cctccaggca gtcgggggaa taaccgccgc acaccttggg gatgctgcgt gtgctgtacg
     2041 tcgcgttgcc cgggtcttcg cgctcgggcg agtaaaccag aaaggcgtcc tggcccacct
     2101 tgaagccgcg ggcttcaatg cggggcagca attcctcgtc cgtggtgccg gggtaggtcg
     2161 tgctttccag cgacagcacc tggccggggc gcatgtaagg ggtcagcgag tcggtggtgt
     2221 cgaggacgaa gctcaggtca ggctcgcggt atttgttcag cggcgtcggc acgcaaagaa
     2281 tcagggcgtc agcctcggcg gcgcaggaaa aatcggttgt gggctcgaag ccctggtcca
     2341 gcgtttgccg gatcgccgcg tcaggaatgc gctcgatgta gctcctgcct tggcgcagcg
     2401 cgtccacctt ggccgggtcg atatcaaaac cgatcacctt gtagccgacc tcgcagtagc
     2461 gcagtaccag cggcaggccg acgtagccca ggccgacgat gccaatcgtg gcttgttttt
     2521 cgttaagtct ttggatcagc ccggttttca atttgaatcc cctgtatcgc gggcatttgg
     2581 gtagtggtgg tgctgacgcg acgccacgaa accgactgcg gctgcaacgt gggcggccag
     2641 ccgggctgga accaggcctg gcgcggtccg ttccgcaagg ccttggacgt cctggcc
//
LOCUS       DAOMFA010000180         2613 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_5895_length_2613_cov_4.505864, whole genome shotgun sequence.
ACCESSION   DAOMFA010000180 DAOMFA010000000
VERSION     DAOMFA010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 2613)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2613)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2613
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5895_length_2613_cov_4.505864"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..301)
                     /locus_tag="PKW44_07990"
     CDS             complement(<1..301)
                     /locus_tag="PKW44_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BPSS1780 family membrane protein"
                     /protein_id="HQR51562.1"
                     /translation="MEIRQVNAGNGWNWIADGFGLFKQSPVIWIALFFVYLLLAMLLS
                     FIPVVGPIILNLLAPVFMAGFMLGCKAIEDGEELEINHLFAGFSRNTSKLITVGGI"
     gene            complement(324..1274)
                     /locus_tag="PKW44_07995"
     CDS             complement(324..1274)
                     /locus_tag="PKW44_07995"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HQR51563.1"
                     /translation="MSVFTTVAPEQLIPWLQDYPLGELRELRGIAAGITNTNYFVTTS
                     SGGYVLTLFEKNTAEELPYFLDLMAHLAGHGIPCPRPVASLDGGYLRQLNGKPAALVS
                     RLHGSSLDTPDSAHCAELGAMLARMHLAGQSFPQRRKNARGPHWRQITAQAVIPFLDA
                     GQRALLEQELAYQSRGPEADLPRGMIHADMFRENVLFEGDRLTGLVDFYYACDGAWAY
                     DLAITANDWCIDDSGNLDEVRLSALLKSYHAVRPLTAAERQAWPALLRAGALRFWLSR
                     LYDLHHPQPGELTYAKDPEHFRHILNQHVQNDDGLRAIWI"
     gene            complement(1354..1695)
                     /locus_tag="PKW44_08000"
     CDS             complement(1354..1695)
                     /locus_tag="PKW44_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2782 domain-containing protein"
                     /protein_id="HQR51564.1"
                     /translation="MFRTRFLILLALIASLPAVAAAPPAGLEPLPEPPPAPEGYEPDP
                     ATEPQVTITKRGEDTVEEYSVNGQVYMMKVTPPNGVPYYLSKDSADGGWQPMDGPGEH
                     YSMPSWVIFKF"
     gene            complement(1751..2479)
                     /locus_tag="PKW44_08005"
     CDS             complement(1751..2479)
                     /locus_tag="PKW44_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019915842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HQR51565.1"
                     /translation="MSASEKLPKISNADTIANERSSQEAWRVFEIMAEFVDATERLKE
                     ISPAVSIFGSARTPPDHPYYRLAEEIARQLSDAGFSVISGGGPGIMEAANKGAYYGES
                     SSIGLNIQLPHEQHTNPYQDISQTFHHFFARKVMFVKFASAYVILPGGFGTMDELMEV
                     LTLVQTGKTRGIPIILVHRPFWQGMLDWFKQVMVTEGMVDAKDMDLIQVKDEPGQVVE
                     AIFKYYETRGFEPSAQEREIQLNL"
BASE COUNT          548 a          755 c          761 g          549 t
ORIGIN      
        1 aaatcccgcc gacggtaatc agtttcgaag tgttgcggct gaacccggcg aacaagtggt
       61 tgatttccag ttcctcgccg tcctcgatcg ctttgcatcc cagcatgaac ccggccatga
      121 aaaccggcgc cagaagattg agtatgatgg ggccgacgac aggaatgaac gacagcagca
      181 tggccagcag cagataaacg aaaaacagcg cgatccagat taccggactc tgcttgaaca
      241 agccgaagcc gtcggcaatc cagttccagc cgttgccggc attgacttga cgtatttcca
      301 tggtgttact ccctgttgta atttcatatc cagatggctc gcaggccatc gtcgttttgt
      361 acatgttgat tcaggatgtg ccggaagtgc tccgggtcct tggcataggt cagctcgccc
      421 ggctgcgggt ggtgcagatc atacaggcgc gacagccaga aacgaagcgc tccggcacgg
      481 agcagggcgg gccaggcctg gcgttctgca gcggtcaacg ggcgcaccgc atgataggat
      541 ttcagcaatg ccgacagcct cacttcgtcc agatttccgg aatcatcgat gcaccagtcg
      601 ttggcagtaa tcgcgagatc gtaagcccag gcaccatcgc aggcataata gaaatccacc
      661 aggccggtca gtcggtcgcc ttcgaacagc acgttttccc gaaacatgtc ggcgtgaatc
      721 attccgcgcg gcagatccgc ttcaggcccc cttgattgat aggccaactc ctgttccagc
      781 aaggcacgtt gacctgcgtc cagaaatgga atcactgcct gcgccgttat ttgccgccaa
      841 tgcgggccgc gggcgttttt cctgcgctgc ggaaaagatt gcccggccag atgcatccgc
      901 gccagcatcg ccccaagttc agcgcaatgc gcgctgtccg gcgtatccag cgagctgccg
      961 tgcagccggc tgaccagggc ggccggcttg ccgttgagct gtctcagata gccgccgtcg
     1021 agactggcaa ccgggcgtgg gcaagggata ccatggccgg caagatgtgc catgaggtcc
     1081 agaaaatacg gcagctcctc ggccgtattc ttttcaaaca gcgtcagcac gtagccaccg
     1141 ctggaagtgg tgacgaagta attggtattg gtgataccgg cggcgatgcc tcgcagttcg
     1201 cgcagctcgc caagcgggta gtcttgcagc catggaataa gctgttcagg cgcgacagtg
     1261 gtgaaaacgg acatcaaatg cttctggtgg attgcaggtg ccgggcgttc cgcagcgccc
     1321 ggagaaagga cgatgccctt ctgcatgcag cttctagaac ttgaatatca cccagctggg
     1381 catggagtag tgttcgccag gcccatccat cggctgccag ccaccatcgg cactgtcctt
     1441 ggagagatag taaggtaccc cgttcggtgg cgtgactttc atcatgtaaa cctgcccatt
     1501 gacgctgtac tcttccaccg tgtcttcgcc gcgtttggtg atggtgactt gcggctcagt
     1561 cgcaggatcc ggttcataac cttccggcgc cggcggcggc tccggcaacg gctccaggcc
     1621 ggctggcggg gccgccgcaa ccgccggcaa cgacgcgatc agtgccagta aaatcaagaa
     1681 gcgggtgcgg aacattaggt agcctcgacc gttaaacagg gtatttgcgg gaattctagc
     1741 agaaagcgcc ttacaaattg agctgaattt cgcgctcctg cgcggagggt tcaaacccgc
     1801 gtgtctcgta gtatttgaag atcgcttcta ccacttggcc gggctcgtct ttcacctgaa
     1861 tcaggtccat atccttggca tcgaccatac cttccgtcac catgacctgt ttgaaccagt
     1921 ccagcatgcc ctgccagaaa ggccggtgta ccaggatgat gggaatgccc cgcgtttttc
     1981 cggtctggac cagcgtgagc acttccatca gttcgtccat ggtgccaaag ccgccgggca
     2041 ggatgacgta ggcggaggcg aacttgacga acatgacttt gcgcgcaaag aaatgatgga
     2101 aagtctggct gatgtcctga tagggattgg tgtgttgctc gtgcggcaac tggatgttca
     2161 ggccgatact ggacgattct ccatagtagg cgcccttgtt ggcggcctcc atgatgcccg
     2221 ggccaccgcc ggaaatgacg ctgaatccgg catcggacag ctggcgggcg atctcctcgg
     2281 ccagccggta atatgggtgg tcgggcggcg tgcgtgcgct gccgaaaatc gacaccgccg
     2341 ggctgatttc cttgagccgc tccgtagcat cgacgaactc tgccataatc tcgaacacgc
     2401 gccacgcctc ctgcgaggag cgctcattgg cgatcgtatc tgcgttgctg atcttgggta
     2461 gcttctctga tgcggacata agggaatcca gaaaaatgaa aactttgttg ttggtggacg
     2521 gttcttccta cctctaccgc gccttccatg ccatccctga cctgcgcaac cacgccaatg
     2581 aacaaactgg cgcgattcag tgcgtagtca aca
//
LOCUS       DAOMFA010000181         2574 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_6035_length_2574_cov_5.442239, whole genome shotgun sequence.
ACCESSION   DAOMFA010000181 DAOMFA010000000
VERSION     DAOMFA010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 2574)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2574)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2574
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6035_length_2574_cov_5.442239"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2574
                     /gene="polA"
                     /locus_tag="PKW44_08010"
     CDS             <1..>2574
                     /gene="polA"
                     /locus_tag="PKW44_08010"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020183718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HQR51566.1"
                     /translation="TLLLVDGSSYLYRAFHAMPDLRNHANEPTGAIQGVLNMLRRLHK
                     DYPSDYSACVFDAKGKTFRDDIYPEYKANRASMPDDLRAQIAPLHETIKAMGWPLIVE
                     DGVEADDVIGALARQAEREGMRVIISTGDKDISQLVNEHITVVNTMRDAFRKVDEVLD
                     PAGVEKKFGIPPGLMVDYLVLIGDTSDNVPGVEKVGPKTAVKWLTQYGSLENIIAHAD
                     EITGVVGENLRKALPWLSTARELITIRCDVGIQECLADLAPQPQDKVRLAELFDRFDF
                     KTWRRELDLPAATTSSSASSQIAGGAASAGDLFSAGAGTTRRYETILSEVRLQAWLDK
                     IAAAPLVCFDTETTSLDPMRAKLVGMSFAVEAGHAAYLPLTHDYVDAPAQLDFAATLA
                     RVKPLLENPSIRKVGQNLKYDKHILANHGIALNGIAHDTLLQSYVLESHRQHNMDDLA
                     MRHLGIKTITFEEVAGKGAKRVGFTQVAVEQAAEYAAEDADITLQLHQTLYPQIERDA
                     KLAHVYRDLEMPVLDILFKIERTGVLIDARMLHAQSHELGQKLLLLEQQAHELAGQPF
                     NLGSPKQLQDILFGKLGIKPLKKTPSGAPSTDEDVLQELALDHPLPKILLEYRGLAKL
                     KSTYTDKLPRSVDGATGRVHTSYSQAVAVTGRLASSDPNLQNIPVRTAEGRRIREAFI
                     APPGCVIVSADYSQVELRIMAHLSRDEGLLKAFAAGEDIHRATAAEIFGCERAEVSSE
                     QRRYAKVINFGLIYGMSAFGLASQLGVERSAAQSYIDRYFARYPGVADYMQRTRQAAH
                     MQGYVETLFGRRLWVPEINSPNGQRRQGAERAAINAPMQGTAADLIKLAMIAVDDWLT
                     E"
BASE COUNT          561 a          833 c          730 g          450 t
ORIGIN      
        1 aactttgttg ttggtggacg gttcttccta cctctaccgc gccttccatg ccatgcctga
       61 cctgcgcaac cacgccaatg aaccaactgg cgcgattcag ggcgtactca acatgctgcg
      121 ccgcctgcac aaggactacc cttctgatta tagcgcctgc gtgttcgatg ccaagggcaa
      181 aaccttccgc gatgatattt atcccgaata caaagccaac cgcgccagca tgccagacga
      241 tttacgcgcc caaatagcgc ctttgcatga aaccatcaaa gctatgggct ggccgctgat
      301 tgtggaggac ggcgtcgagg ccgacgacgt gatcggcgcc ctcgccagac aggccgagcg
      361 cgaaggcatg cgggtgatta tctccaccgg cgacaaggat atttcgcaac tggtgaatga
      421 gcacattacc gtcgtcaaca ccatgcgcga tgcgttcaga aaagtcgatg aggtcttgga
      481 cccggcaggt gtcgaaaaga aattcggcat accgcccggc ctgatggtgg attacctcgt
      541 tttgattggc gacacttcag acaatgtgcc gggcgttgag aaagtcggcc ccaaaaccgc
      601 ggtgaaatgg ctcacgcaat atggctcgct ggaaaacatc atcgcgcatg ccgatgagat
      661 cacgggcgtg gtgggcgaga acctgcgcaa ggcgttgccc tggctgtcca ccgcgcgcga
      721 gctgattacc atacgctgcg atgtgggcat acaggagtgc ctcgctgacc ttgccccgca
      781 acctcaagat aaagtcagac tggctgaatt gtttgaccgc tttgatttta aaacctggcg
      841 gcgcgaactg gatttgccgg ctgcaacaac gtcctcatca gcctcatcgc aaatcgcagg
      901 gggggcggca agtgccggcg atttgttctc cgctggcgcc ggcactaccc gccgttacga
      961 aaccatcctg agcgaagtcc ggctgcaagc ctggctggac aagattgccg ccgcgccctt
     1021 ggtctgcttc gataccgaaa ccaccagcct cgaccccatg cgggcaaagc tggtcggcat
     1081 gtcgttcgcg gtggaagccg gccatgctgc ctacctgccg ctgacgcacg actacgtaga
     1141 cgcgccggcg cagctggatt tcgccgctac gctggcgcgg gtcaagccgc tgctggaaaa
     1201 cccttctatc cgcaaagtcg gccagaacct caagtacgac aagcacatcc tcgccaacca
     1261 cggcatcgcg ctcaacggca tcgcccacga caccctgctg caatcctacg tgctggaaag
     1321 ccaccgccag cacaacatgg acgacctcgc catgcgccac ttggggatca agactatcac
     1381 tttcgaggaa gtcgcaggca agggcgccaa gcgggtcggc ttcacccagg ttgccgtgga
     1441 acaggccgcc gaatacgccg ccgaggatgc cgacatcacg ctgcagctgc accagacgct
     1501 atacccgcag atcgaacgcg acgccaagct ggcgcacgtc taccgcgacc tggaaatgcc
     1561 ggtgctggac atcctgttca aaatcgagcg cacaggcgtg ctgatcgacg cccgcatgct
     1621 gcacgcgcaa agccacgaac tggggcagaa actgctttta ctggaacagc aggcgcatga
     1681 gttggccggg caaccgttca acctcggttc acccaagcaa ctgcaggata tcttgttcgg
     1741 caagctcggc atcaagccgc tgaagaaaac gccttccggc gcaccctcca ccgacgaaga
     1801 cgtgctgcag gaactggcgc tggaccatcc gttgccgaaa attctgctgg aataccgcgg
     1861 actggccaag ctcaagtcta cctacaccga caagctaccg cgctcggtgg atggcgccac
     1921 cgggcgcgtg cataccagct acagccaggc agtggcggtg acaggacgcc tggccagctc
     1981 cgatcccaac ttgcaaaata tcccggtgcg taccgccgaa ggacgccgca tccgcgaagc
     2041 cttcatcgcg ccgcccggct gcgtcatcgt ttccgccgac tattcgcagg tcgagctgcg
     2101 catcatggcg cacctttcca gagatgaagg cttgctcaag gccttcgccg ccggcgagga
     2161 catccaccgc gccaccgccg ccgaaatctt cggctgcgaa cgcgccgaag tcagcagcga
     2221 gcagcgccgc tacgccaagg tcatcaactt tggcctgatc tacggcatgt ctgcctttgg
     2281 gctggccagc cagcttggcg tggagcgcag cgcggcgcag agctatatcg accgctactt
     2341 cgcccgctat cccggcgtgg ccgattacat gcagcgcacg cgccaggccg cgcacatgca
     2401 aggttatgtg gaaacattat tcggaagaag attgtgggtg ccggaaatca acagcccgaa
     2461 tggacagcgc cgccaaggcg ccgagcgcgc ggccatcaac gcccccatgc aaggcaccgc
     2521 tgccgacctc atcaagcttg ccatgatcgc cgtggatgac tggctcaccg aaca
//
LOCUS       DAOMFA010000182         2538 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_6158_length_2538_cov_5.408780, whole genome shotgun sequence.
ACCESSION   DAOMFA010000182 DAOMFA010000000
VERSION     DAOMFA010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 2538)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2538)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2538
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6158_length_2538_cov_5.408780"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..432)
                     /locus_tag="PKW44_08015"
     CDS             complement(<1..432)
                     /locus_tag="PKW44_08015"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011381273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HQR51567.1"
                     /translation="MSGNTFGTLFTVTTFGESHGAGIGCVVDGCPPGLSLQEADIQAE
                     LDRRKPGTSPRQVTQRKEPDTVEILSGVFEGKTTGTPIALLIRNQDQRSQDYGTIAET
                     FRPGHADYTYWQKYGIRDYRGGDRSSARETAARGASGGIAKK"
     gene            503..>2538
                     /locus_tag="PKW44_08020"
     CDS             503..>2538
                     /locus_tag="PKW44_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HQR51568.1"
                     /translation="MKIHQFSPEQALVSLNSSLNGLASDEAARRLAEYGANQVEVLRE
                     EPLWLALAKEFTHFFALILWFAAGLAFFAEHQMPGEGMATLGFAILGVILINGLFSFW
                     QEYRAEQAIAALQKLLPQQIKAIRNGRLTLIAATELVPGDVVLLQEGDNVPADCRLLQ
                     AFSLRVNNATVTGESLPKGRDALASPTEDILSSRNVLLAGTSVVAGEGHAVVFATGMH
                     TEFGKIAHLTQTSGEVLSPLQREIIRLSRLVAMLATLIGVAFFVIGRAMGLPFWESLL
                     FGIGIIVANVPEGLLPTVTLALAMATQRMAKRNALIRHLPSVEALGAATVICTDKTGT
                     LTQNKMRVEQLWLGGELLATDAPAGKTPHYRRLLEDAALCHNLKQSSGTWLGDPMEVA
                     LVELAKVGLEALPAWPKTDEIAFDTDRKRLSTVHDTPEGRILYCKGAPETVLPRCTRA
                     QSDADVVALDAEWKARILAAQEAMTGQGLRVLALAWRELPDGRPHDPEQEMVLAGLVG
                     LADPPRPEVPDAIRKCHEAGIKVIMVTGDHPHTAVAIARQIGLVVSRNPSVISGDELK
                     KLSNTQLQIALDAKEILFARVGADQKMRIVQALKRKKHIVAVTGDGVNDAPALKAADI
                     GISMGLCGTDAAKAAADMILLDDNFASIVAAVEEGRAVFDNIRKFLAYILTSNIP"
BASE COUNT          530 a          808 c          758 g          442 t
ORIGIN      
        1 ttttttggca atcccccccg atgccccgcg cgctgccgtc tcgcgggcgc tggaacggtc
       61 gccgccgcgg tagtcgcgga tgccgtattt ctgccagtag gtgtaatcgg catggccagg
      121 gcggaaggtc tcggcaatgg tgccgtaatc ctggctgcgc tgatcctggt tgcggatcag
      181 cagcgcaatc ggcgtgccgg tagtcttacc ctcgaacacc ccggacagga tttcgacggt
      241 atcgggctcc ttgcgctgtg tcacttggcg cggggaggta ccaggctttc gacgatccag
      301 ctcggcctga atatcggctt cttgcaggct caaccccggc gggcagccgt ccaccacgca
      361 gccgatgccg gcgccatgag actcaccaaa agtagtgacc gtaaacaggg tgccaaacgt
      421 attgccagac atattcaagg ctcgtaagta aacgctgaca agtttaacat attcctcatc
      481 ccaccccctg ccagaaggca gcatgaaaat tcatcaattc agtccggaac aagcacttgt
      541 cagcctcaac agcagcttga acgggcttgc ctcggacgaa gcagcgcgac ggctggcaga
      601 atatggcgcc aaccaggtag aagtactgcg tgaagagccc ttgtggctag ccctggctaa
      661 ggaattcacc cacttctttg cactgatttt gtggtttgct gcgggactgg catttttcgc
      721 tgaacaccag atgccggggg agggcatggc cacgctgggt ttcgccatac tcggcgtcat
      781 cctcatcaac ggcctattct cgttctggca ggaataccgc gcggaacaag ctatcgcggc
      841 gctgcaaaaa ttgctgccgc agcaaatcaa ggccatacgg aatggcagac tgacgctgat
      901 cgccgccacc gaactggtac caggcgacgt cgtgctgctg caggaaggcg acaacgtgcc
      961 ggccgattgc cgactgctgc aagccttttc cttgcgtgtg aacaacgcca cggtgactgg
     1021 cgaatctctg cccaagggac gcgatgcgct ggcttccccg accgaggaca tcctaagcag
     1081 tcgcaacgtg ctgctggctg gcaccagcgt ggtggcaggc gaaggtcatg cagtggtgtt
     1141 tgctacaggc atgcatacgg aattcggaaa aattgcacat ttaacccaaa cctccggcga
     1201 ggtactgtcg ccgctgcaac gggaaatcat ccgcctgagc cgactggtgg cgatgctggc
     1261 gaccttgatc ggcgtggcct tcttcgtcat cggccgcgcc atgggactgc cgttctggga
     1321 aagcctgctg ttcggcatcg gcatcatcgt cgccaacgtg ccggaagggc tgctgccgac
     1381 ggtgacgctg gcgctggcga tggccaccca gcgcatggcc aaacgcaacg cgctgatccg
     1441 ccacctgccc tcggtcgaag cgctgggtgc ggccaccgtg atctgcaccg acaagactgg
     1501 cacgctcacg caaaacaaaa tgcgagtgga gcaactgtgg ctgggcggag aactcctggc
     1561 aacagatgcg ccggcaggaa aaacgccgca ttaccggagg ctgcttgagg atgccgcact
     1621 gtgccacaac ctcaagcaaa gcagcggcac ctggctgggc gacccgatgg aagtggcact
     1681 ggtggaattg gccaaagttg ggctggaagc cctgccggcc tggccaaaaa ccgacgaaat
     1741 cgctttcgat accgaccgca aaagactctc caccgtgcat gacacgccgg agggcaggat
     1801 actgtactgc aagggcgcgc ctgaaaccgt gctgccacga tgcacccggg ctcagtccga
     1861 tgccgatgta gtagctctgg atgcggaatg gaaggcccgc atactggcag cacaggaagc
     1921 gatgaccggc caggggttac gtgtgctggc actggcttgg cgcgaactac ccgacggccg
     1981 tccgcacgac cccgagcagg agatggtgct ggcaggcctc gtcggcctgg ccgacccgcc
     2041 gcgccccgaa gtgccggacg ccatccgcaa atgccatgaa gccggcatca aggtcatcat
     2101 ggtcaccggc gaccacccgc acaccgccgt cgccatcgcg cgccagatcg ggctggtggt
     2161 ttccagaaac cccagcgtca tcagcggcga cgaactcaag aaactttcca acacgcagtt
     2221 gcagatcgcg ctggacgcga aggaaatcct cttcgcccgc gtcggcgccg accagaaaat
     2281 gcgcatcgtg caggcgctca agcgcaagaa acacatcgtc gccgtcaccg gcgacggcgt
     2341 caacgacgcc cccgcgctca aggccgccga catcggcatt tccatgggtc tctgcggcac
     2401 cgacgccgcc aaggccgccg ccgacatgat cctgctcgac gacaacttcg ccagcatcgt
     2461 cgccgcggtc gaggaaggcc gcgcggtgtt cgacaacatc cgcaagttcc tcgcctacat
     2521 cctcacctcc aacattcc
//
LOCUS       DAOMFA010000183         2512 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_6264_length_2512_cov_5.676435, whole genome shotgun sequence.
ACCESSION   DAOMFA010000183 DAOMFA010000000
VERSION     DAOMFA010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 2512)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2512)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2512
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6264_length_2512_cov_5.676435"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..947)
                     /locus_tag="PKW44_08025"
     CDS             complement(<1..947)
                     /locus_tag="PKW44_08025"
                     /inference="COORDINATES: protein motif:HMM:NF016763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HQR51569.1"
                     /translation="MEPKVIAGHPADTSRRNHRIAVGLLLSLIVYGSLYPFSWNFAQP
                     QDFIYWAPLGLVDVVENVILFLPLGWLLAWHYHGQGRQRAGFVAWFPITLIVAGILQW
                     LQKFLPRTPALSDIVFNMLGFAAGWLAGMISGNSLTRLSRRYLNPHVADRFALVMVVL
                     WVAAELYPFIPTLDQSSVAYNVKSLWQQDLWQPRRMLLHMAVAVIGLEALAHLVRSVA
                     EERLARLLAGIATLAILGGKFVIINQAPGLAVVLGIAAGAAVWQGIDRMEERPRLWTL
                     LAIATAAYLLHAIWPPQWNNRPSAMHWLPFSSSLSGSIEA"
     gene            1352..1858
                     /locus_tag="PKW44_08030"
     CDS             1352..1858
                     /locus_tag="PKW44_08030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR51570.1"
                     /translation="MSSKNDLFRTINARSPGIVEQLITGWGSLQFSEYVNDLLKDAQR
                     SAKPQLKEDVISALAALRAEHDREFPQFAILTEDLVVERLEQTPYFQTVNAKFPRIAR
                     RLVATWGHAAFGEYINDLLNDNRAGRQGFPEDIMLALFKLSEEHDKEFPEYVLKITDI
                     WSLTNKIY"
     gene            complement(2078..>2512)
                     /locus_tag="PKW44_08035"
     CDS             complement(2078..>2512)
                     /locus_tag="PKW44_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019186275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HQR51571.1"
                     /translation="IISVASLVGFGAVVAALPAFAAVAEWVLSIGGSPLVSLAVSTNV
                     LSALTGSASGGMTIALEALGPIYLQIAAVQGIDPALLHRIAVIGAGTLDSLPHNGAVI
                     TLLAVCGSTHRKSYFDIVMCCVVSAMLALIAVIIIGGMFGSF"
BASE COUNT          533 a          762 c          765 g          452 t
ORIGIN      
        1 gcttcgatcg accccgacaa cgaggaggaa aacggcagcc agtgcatcgc gctgggccgg
       61 ttgttccatt gcggtggcca gatggcgtgg agcaggtagg ctgcggtggc gatcgcgagc
      121 aaggtccaca agcgcggcct ttcctccatc cgatcgatgc cctgccacac cgcggccccc
      181 gccgcgatgc ccaagaccac ggccaatccg ggcgcctggt tgatgatgac gaacttgccg
      241 cccaatatcg ccagcgtcgc gatgcccgcc agcagtctgg caaggcgctc ctcagccacc
      301 gagcgaacga gatgggccag cgcctccagt ccgatcaccg ccactgccat gtgcagcagc
      361 atgcggcgtg gctgccacag atcctgttgc cacagcgact tcacgttgta ggcgacagat
      421 gactggtcga gcgtgggtat gaaggggtac agttcagccg ccacccagag cacgaccatc
      481 acgagcgcaa agcgatcggc gacatgcggg ttgaggtagc gccgcgacag ccgggtcagg
      541 ctgttcccgg agatcatccc ggccagccag ccagccgcaa accccagcat gttgaagacg
      601 atatcggaca gcgccggggt gcgtggcagg aatttctgca gccactgcag gatgccggca
      661 acgattagcg tgatcgggaa ccaggcgaca aaacctgccc gctggcggcc ctggccatgg
      721 tagtgccagg cgagcagcca gcccagcggc aggaacagga tcacgttctc gacgacgtcg
      781 accagaccca gcggtgccca ataaatgaag tcttgcggct gcgcgaagtt ccaggaaaac
      841 ggatacagtg acccataaac gatcaatgac agcaacagcc cgacggcgat cctgtggttg
      901 cgtcggcttg tgtcggccgg gtgtcctgct ataactttgg gttccacgcg ggtttctcgt
      961 attggtcgag gcgcagtgac ggtttcacgg caatgcactc atgctccatg aatttcacca
     1021 cgttcccggc atcgaccgca acaatcagcg cgaactgtca tttttgccgg ttcagggtgg
     1081 caagcgaccg attgttcgcg tctgccgtga tgggtgacca ggcaactcca caaccctgca
     1141 aggatccggc tacgggcgac gtgcgtgtcc aacccgtttc ccatgttgac tgcctcatgt
     1201 actgcacacg tctccgctgc gggccgtgta gtatcgcaag gcccacagac tgcaactggc
     1261 gcgggaatgt tttcgtttcc gctttgccgt cgtaatctga tataaatcgt gttttcgtga
     1321 ctgtgagtcg gacaagtttg tgggggttaa aatgtcgagc aagaatgacc tgttcagaac
     1381 tatcaatgcg cgatctcccg gcattgtcga gcaactcatt accggctggg gaagtctgca
     1441 gttcagcgag tatgtcaatg acctgctcaa ggatgcgcag aggagtgcca agccgcaact
     1501 caaggaagac gtaatctcgg ctctggcagc actgcgggcc gagcatgacc gtgagttccc
     1561 gcaattcgca attctgaccg aggacctggt tgtggaacgg ctcgagcaga cgccgtattt
     1621 ccaaacggta aatgcgaaat ttccgcgaat tgcgcggcgc cttgttgcga cctggggtca
     1681 cgccgctttc ggtgaatata tcaacgactt gctcaacgac aaccgggcgg ggcggcaagg
     1741 gtttccggag gacatcatgc tcgccctttt caagttgtcg gaggaacatg acaaggagtt
     1801 cccggaatac gtcctcaaga ttacggatat ctggtcgctg accaacaaga tctactgacc
     1861 gttccgggtt ggctccggcc aggtactcca agcagggtgg tcacagggag acgacatctc
     1921 ccggccacta ccggtcggct ggacgaaagc gataagcatg cgcgcgaacg ctctgcccga
     1981 agccgcggag ccttccgctt ttgcagccgc cggaaatgcc cggcctccgg gaatcgggtg
     2041 tcacacgcgc tgccgatgcg ggcgcctcat ttcactttca gaacgaaccg aacatcccgc
     2101 cgatgatgat gaccgcgatc aacgccagca tcgcgctgac gacgcagcac atcacgatgt
     2161 cgaaatagct cttgcggtgc gtcgatccac agacagccaa cagggtgatg actgcgccgt
     2221 tgtgcggcag gctgtccagg gtgccggcgc cgatcaccgc gatgcgatgc agcagcgccg
     2281 gatcgatacc ctgtacggcg gcaatctgga gatagatcgg gccgagtgcc tccagggcga
     2341 tggtcatgcc gccggaagca gaaccggtta gcgccgatag aacgttggtc gatacggcca
     2401 gcgaaaccag cgggctgccc ccgatcgaca acacccattc ggccaccgcc gcgaaggccg
     2461 gcagcgccgc gacgaccgcg ccgaagccaa ccaggctggc gacgctgatg at
//
LOCUS       DAOMFA010000184         2491 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_6355_length_2491_cov_3.956486, whole genome shotgun sequence.
ACCESSION   DAOMFA010000184 DAOMFA010000000
VERSION     DAOMFA010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 2491)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2491)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2491
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6355_length_2491_cov_3.956486"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1448)
                     /locus_tag="PKW44_08040"
     CDS             complement(<1..1448)
                     /locus_tag="PKW44_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HQR51572.1"
                     /translation="MRLTHLKLAGFKSFVDPTTLHVHGQRIGVVGPNGCGKSNVMESV
                     RWVLGESSAKEMRGDSMQDVIFNGSGNRKPVSRASVELIFDNSLGGATGEWSQYAEIS
                     VKRVLQRDSGSTYYINNTPVRRRDVADLFLGTGLGGRAYAIIGQNTISRIVEAKPDEL
                     RVFLEEAAGISKYKERRRETELRLRDTRDNLARAEDIRRELGTQIERLESQALVAQQY
                     HELQHQLGIAQSQFWLLKKRDAAQAWEKSRRQVERLVNDLEAQTASLRKIESELEQLR
                     LQQHACTESVQAAQGHYYEANARVSTLEQQLRHEQENHERLTAQLAQIVTEQAKVAAQ
                     QQEYRQQLQQQYAAQQHAAAAAGEAQRALEARRARLPQAEQGWRDAQAGFSQVQNAIL
                     QADQKIQLERTHFDHARKTLAELGQRMQRLVEDESRLVLPDAAQVAARLQDCERGQVE
                     LARLEADLAQQAQAEQERAEQVGMLQQAVHQKA"
     gene            1515..1934
                     /gene="queF"
                     /locus_tag="PKW44_08045"
     CDS             1515..1934
                     /gene="queF"
                     /locus_tag="PKW44_08045"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HQR51573.1"
                     /translation="MPSQPSQTLETFDNPNPGRDFHIHMEIPEFTCLCPKTGQPDFAT
                     LHLDYIADRKCVELKSLKLYIWSFRDQGAFHEAVTNRILDDLVAATQPRFMRITAKFY
                     VRGGIFTNVIAEHRKPGWQPLPYVELGQFESQSNTRG"
     gene            1938..2471
                     /locus_tag="PKW44_08050"
     CDS             1938..2471
                     /locus_tag="PKW44_08050"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HQR51574.1"
                     /translation="MKILLLVAVLGLVLLLFRSVSMAGQLPQLGQAAPDFSLPDQNGK
                     THRLSDYAGQWLVLYFYPKDDTPGCTKEACSFRDDLQQLEKLGAKVVGISVDDTDSHA
                     KFAAKYHLPFPLLADNDAKVAENYRALNNLLVIKIARRYTFLIDPQGKIAKNYLSIDT
                     SRHSLEIIEDLKRLTKQ"
BASE COUNT          470 a          807 c          664 g          550 t
ORIGIN      
        1 gctttctgat gcaccgcctg ttgcagcatt ccgacctgct cggcccgctc ctgctcggct
       61 tgtgcctgtt gcgccagatc cgcttccagc ctggccagct ccacctggcc ccgctcgcag
      121 tcctgcaacc tcgccgccac ttgcgcggca tccggcagca ccagcctgga ttcgtcctct
      181 accagccgct gcatacgctg ccccagttct gccagagttt tcctggcgtg gtcaaagtgg
      241 gtgcgttcca actggatttt ctgatcggct tgcagaatcg cgttctgcac ctggctgaaa
      301 ccggcctgtg cgtcacgcca gccttgctcg gcctgcggca gtcgggcacg cctggcttcc
      361 agcgcccgct gcgcttcccc agccgctgcc gccgcgtgct gctgcgccgc gtactgctgt
      421 tgcagttgct gacgatattc ctgttgttgc gcagctacct tggcttgctc ggtgacgatc
      481 tgggccagtt gcgcggtcag gcgctcatga ttttcctgct cgtgccgaag ctgctgctcc
      541 agcgtggaga cccgggcatt ggcttcgtag tagtgccctt gcgcggcctg caccgattcg
      601 gtgcaggcat gttgctgcag ccgcagctgt tccagttcac tctcgatttt gcgcaaactg
      661 gcagtctgcg cctccagatc gttgaccagt cgctccacct ggcgccgcga tttttcccaa
      721 gcctgtgcgg cgtcgcgctt cttcagcagc cagaactggc tctgcgctat gcctaattga
      781 tgctgcagct catggtactg ttgcgccacc agcgcctgtg attccagccg ctcgatctgt
      841 gtgcccaatt cgcgccggat atcctccgcc cgggccaggt tgtcacgagt atcacgcaaa
      901 cgcagctcgg tttcccggcg gcgttccttg tattttgaga tgccggcggc ctcttccaga
      961 aagacacgca gttcatcggg cttggcttcg acgatgcgcg aaatggtgtt ctggccaatg
     1021 atggcgtagg cgcggccgcc cagcccggtc cccagaaaca ggtcggcaac gtcgcgccgc
     1081 cggaccgggg tattgttgat gtagtaagtg gagccggaat cgcgctgcag cacgcgcttg
     1141 acggagattt ctgcgtattg cgaccattcc cccgtcgcgc cgcccaggct gttgtcaaaa
     1201 atcagttcca cactggcgcg agatacgggt tttctattgc cggaaccgtt gaaaatcacg
     1261 tcctgcatcg aatcgccgcg catttccttt gcgctggatt cacccagcac ccagcgcact
     1321 gattccatga cgttggattt gccgcagccg ttgggcccga ccacgccgat gcgctggccg
     1381 tgaacgtgca gcgtggttgg gtcaacgaag gacttgaaac cggcgagctt gaggtgagtc
     1441 aggcgcaatt gggcagcact tctttagggt tgtataattc agcttaaacc acagcgattt
     1501 atacgggaca caccatgcca agtcaaccca gccagaccct ggaaaccttc gataacccca
     1561 accccggacg cgatttccac atccacatgg aaatcccgga atttacctgc ctgtgcccta
     1621 agaccggaca gccggatttc gccaccttgc acctggatta catcgccgac cggaaatgcg
     1681 tggaactgaa gagcctcaag ctctacatct ggtcgttccg cgaccagggc gcctttcacg
     1741 aagccgtcac caaccgcatt ctggacgatc ttgtagcggc tacgcagccg cgtttcatgc
     1801 gcattaccgc caagttttac gtacgtggcg gcattttcac caacgttatc gccgaacacc
     1861 gcaagccggg ctggcaacca ttgccctacg ttgaactcgg ccagttcgag tcccagtcca
     1921 acacccgggg ctgaatcatg aaaattcttc tgctcgttgc cgtactaggc ctcgtcctgc
     1981 tgctattccg ctcagtcagc atggcaggac aactgccgca attggggcaa gcggcaccgg
     2041 atttctcgct gccagaccag aatggcaaga cgcatcgcct ctccgactac gcgggccagt
     2101 ggctggtact atatttctac cccaaagatg acactccggg ctgcaccaag gaggcctgca
     2161 gtttccgtga cgatttgcag caactggaaa aactgggcgc caaggtggtc ggcatcagcg
     2221 ttgacgacac cgactcccat gcgaaattcg ccgccaagta ccatttgccc ttccctttgc
     2281 tcgccgacaa tgacgccaag gtggccgaaa actaccgcgc cctgaataat ctcctcgtga
     2341 tcaaaattgc cagacgctat actttcctca tcgatccaca gggcaaaatc gccaaaaact
     2401 atctgagcat cgatacctcg cgccactccc tggaaatcat cgaggattta aagcgcctga
     2461 ccaagcagta acaaacgtat tcgggcgttc a
//
LOCUS       DAOMFA010000185         2480 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_6391_length_2480_cov_4.637938, whole genome shotgun sequence.
ACCESSION   DAOMFA010000185 DAOMFA010000000
VERSION     DAOMFA010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 2480)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2480)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2480
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6391_length_2480_cov_4.637938"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..409)
                     /locus_tag="PKW44_08055"
     CDS             complement(<1..409)
                     /locus_tag="PKW44_08055"
                     /inference="COORDINATES: protein motif:HMM:NF015810.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor negative regulatory protein"
                     /protein_id="HQR51575.1"
                     /translation="MKDQISALMDDELDTATSSHLFAALNADDKLSECWALYHAIGDS
                     MRGDPQFTPDFQKRLMRRLAQEPTVLAPRKPSYKPSFMLSAAASVAAVLFVGWMVLQQ
                     QAQSLPAAPSFAPNNVSAEPARSYLVVQHEPSPE"
     gene            complement(409..1008)
                     /gene="rpoE"
                     /locus_tag="PKW44_08060"
     CDS             complement(409..1008)
                     /gene="rpoE"
                     /locus_tag="PKW44_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019917809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HQR51576.1"
                     /translation="MGDREIDQELVERAQRGDKRAFGLLVEKYQRKLGRLLGRMIRDQ
                     AEVEDVVQEAFIKAYRALPNFRGESAFYTWLYRIGINTAKNYLVAMGRRPRVSTEIEV
                     EDAENFEDGDELRTIATPETELMSKQVAQTVNDTVAALPEELRTAITLREIEGLSYEE
                     IASLMDCPIGTVRSRIFRARETIAEKLRPLLETQENRRW"
     gene            1146..>2480
                     /gene="nadB"
                     /locus_tag="PKW44_08065"
     CDS             1146..>2480
                     /gene="nadB"
                     /locus_tag="PKW44_08065"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HQR51577.1"
                     /translation="MLRFDVLIIGSGLAGLTMALQLAPHKKVCLVTKRQLTDSASSWA
                     QGGIAAVLTDDDSLEAHIQDTLIAGAGLCDPEVTRHVVQHGRAMVEWLIDQGVPFTRD
                     EHSESGFHLTREGGHSKRRIIHAADATGHAVQETLAQKVRNHPGITLLEEHIAVDLIT
                     ARKLGKRKDHCLGAYVLDKKHGKVLTISAQHTALACGGLGKVYLYTTNPDVATGDGVA
                     MAWRAGCRVANMEFIQFHPTCLYHPQAKTFLITEAVRGEGGLLKLPDGTRFMPAHDPR
                     EELAPRDVVARAIDFEIKKRGIDCVYLDISHRSADFIHAHFPNIYQRCLELGIDITRQ
                     PIPVVPAVHFSCGGVMTDHIGSTDLPNLYAIGETACTGLHGANRLASNSLLECLVFGN
                     SAAEDIFSKPDVVLPELPDWDERRVTDAEEEVIITQNWDELRRFMWNYVGIVR"
BASE COUNT          528 a          763 c          680 g          509 t
ORIGIN      
        1 tctcgggtga tggctcatgc tgcaccacca aataagaccg ggcgggctcg gctgaaacat
       61 tgttcggagc aaaagaggga gctgccggaa gggattgcgc ttgctgctgc aagaccatcc
      121 atccgacaaa cagaaccgcg gcaacagaag ccgcagcgga aagcatgaat gatggtttgt
      181 aagaaggttt acgcggtgcc aggacagtag gctcctgcgc cagtcgccgc atcaggcgct
      241 tttggaaatc aggggtgaat tgaggatcgc cacgcatgct gtcaccgatc gcgtggtaca
      301 gagcccagca ttcgcttagc ttgtcatcgg cgttgagggc ggcaaacaga tgcgatgacg
      361 ttgccgtatc cagttcatca tccatcaagg cagaaatttg atctttcatc accatctcct
      421 gttttcctgc gtctccagca ggggacgcaa cttttctgct atggtttcgc gtgcccgaaa
      481 aatacgggac cgcaccgtgc cgataggaca atccatcagg cttgcaattt cttcatagct
      541 caggccttct atctcgcgta gcgtaatcgc ggtacgcaat tcctcgggca aggccgccac
      601 cgtatcgttg acggtttggg caacttgttt actcatgagt tcagtttccg gcgtggctat
      661 cgtacgcaat tcgtcaccat cctcgaaatt ctcggcatcc tctacttcga tttcggtact
      721 gacgcgcgga cggcgtccca tcgccaccag ataattcttg gcggtattga tgccgatgcg
      781 gtacagccag gtatagaagg cgctttcgcc acggaagttg ggcaacgcac ggtaggcctt
      841 gatgaaggcc tcctgcacca cgtcttccac ctccgcctgg tcacgtatca tgcggcccag
      901 caagcgtccc agcttgcgct ggtatttttc caccagcaaa ccgaaggcac gcttgtcgcc
      961 gcgctgtgcg cgttcgacca gttcctgatc tatttcccga tcgcccatgc gggcgttccc
     1021 cctgatgaat tattatttgg catgttaagt ttgagtataa cgcgcctcgc aaagctactg
     1081 taagcctatg cgcagcttaa agacagcagt acacttcgct tggttcactt tgattgaaga
     1141 aaatcgtgtt gcgtttcgac gttctcatca tcggcagcgg gctggccgga ctgacgatgg
     1201 cactccaact agccccgcac aagaaagttt gcctcgtcac caagcgccag ttgaccgaca
     1261 gcgcaagtag ctgggcacaa ggcggtattg ccgcggtgct aaccgacgac gactcactgg
     1321 aagcacacat ccaggacact ctcatcgccg gcgccggctt gtgcgacccc gaagtcacgc
     1381 gacatgtcgt ccagcatggc cgcgccatgg tggaatggct gattgatcaa ggcgtgcctt
     1441 tcacgcgcga cgagcatagc gagagcggct tccacctcac acgcgaaggc ggacacagca
     1501 agcgccgcat catccacgcc gccgatgcca ctgggcacgc ggtgcaggaa acgctggcgc
     1561 aaaaagtgcg caaccatccg ggcattactc tgctggaaga gcacatcgcc gtcgacctca
     1621 tcaccgcacg caagcttggc aaacgcaagg atcactgcct gggcgcctac gtgctggaca
     1681 agaaacacgg caaggtgctg accatttcgg cgcagcatac cgcactcgcc tgcggcggcc
     1741 tgggcaaggt ttatctctac accaccaacc ccgacgtcgc caccggcgat ggggtagcca
     1801 tggcttggcg cgcgggctgc cgggtagcca acatggaatt catccagttc catcccacct
     1861 gtctctatca cccgcaggcc aagaccttcc tcatcaccga ggcagtccgc ggcgaaggcg
     1921 gcctgctcaa actgccggac ggaacgcgtt tcatgccggc acatgatcca cgcgaagaac
     1981 tggctccgcg cgatgtggta gcgcgtgcca tcgacttcga gatcaagaag cgtggtatcg
     2041 actgcgttta tctggacatc tcccaccgct cggcagattt catccatgcg catttcccca
     2101 acatctacca gcgctgccta gaactcggca tcgacatcac gcgccagccc attccggtgg
     2161 taccggccgt ccatttcagc tgcggcggcg tgatgaccga ccatatcggc tccaccgatc
     2221 tgcccaatct gtacgccatt ggcgaaactg cctgcactgg tctgcacggc gcaaaccgcc
     2281 tggccagcaa ctcgctgctt gagtgtctgg tgttcggcaa ttccgccgca gaggatatct
     2341 tctccaagcc ggatgtcgtg ctgccggaac tgccggactg ggacgaacgc cgcgtcaccg
     2401 atgccgaaga ggaagtgatc attacccaga actgggacga actgcggcgc ttcatgtgga
     2461 actacgtcgg catcgtgcgc
//
LOCUS       DAOMFA010000186         2464 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_6452_length_2464_cov_4.682441, whole genome shotgun sequence.
ACCESSION   DAOMFA010000186 DAOMFA010000000
VERSION     DAOMFA010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 2464)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2464)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2464
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6452_length_2464_cov_4.682441"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..621)
                     /locus_tag="PKW44_08070"
     CDS             complement(<1..621)
                     /locus_tag="PKW44_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013149278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HQR51578.1"
                     /translation="MDKRVGIGWIATLVTLLVLTGCGREPLHQSQAYVFGTLVDISIY
                     GESEERARELSGHVQQEFQRLHRQLHAWQAGSELDRLNVAFAAGKPAAVSPELAGMLA
                     DAAKLSEQSGGLFNPAIGHLIQLWGFQRDEFAPVHPDPEEIAKWVNANPRMADIVVEN
                     SQAASRNPAVKLDLGGYAKGWALDLAADYLRRQGVKGALVNIGGNII"
     gene            complement(627..1565)
                     /gene="gshB"
                     /locus_tag="PKW44_08075"
     CDS             complement(627..1565)
                     /gene="gshB"
                     /locus_tag="PKW44_08075"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HQR51579.1"
                     /translation="MKLAFILDPLDSLKTDKDTSVAIMREAERRGHILFVAEQGDLMM
                     RGPHAALKAVPFSFDDGDTWYQTGDKTEHAPEQFDAVLMRKDPPFDTEFIYSTYLLEL
                     AEKQGARVFNRPCAIRDWNEKLSVARFPQYAPEFLVTRDDAEIRAFLAEHGDIVVKPL
                     DGMGGSSVFRLHSADHNIGVILETITHYERRTVMAQRYLPEIKAGDKRILVIDGEPLP
                     YALARIPKAGETRGNLAAGGTGVGQPLSVRDREIATTVGIELKKEGLFLVGLDVIGDH
                     LTEINVTSPTGMVEIAAQTDCDPAAVFMDALEGLCT"
     gene            complement(1562..>2464)
                     /gene="gshA"
                     /locus_tag="PKW44_08080"
     CDS             complement(1562..>2464)
                     /gene="gshA"
                     /locus_tag="PKW44_08080"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018078172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HQR51580.1"
                     /translation="SSPRRSAASASTMRRAAALGLPHGGQLELEPVRREGNRLVVDGF
                     ESCAVLLNNDLSGGIPDILRDLEQSLIPPLHAGWAVRRKSRHFSAYTWVAQKFAELID
                     IDPWLIDPMFASCGMIDFHARVGEDCLASQVSDLLSRIGAKYRKYGVEQEPFVIVKAD
                     AGTYGMGIMTVKSPDDVRGLNRKQRNKMSVVKEGLQVQEVIIQEGVYTFESINDAVAE
                     PVVYMMDHYVVGGFYRVHTGRGMDENLNAPGMHFVPLAFDTACSLPDHAGMPDAAPNR
                     FYAYGVIARLALLAAAIELEQTAP"
BASE COUNT          452 a          779 c          763 g          470 t
ORIGIN      
        1 gatgatgttg ccgccaatgt tgaccagcgc ccccttgacc ccctgtcggc gcagataatc
       61 cgccgcaaga tccagcgccc agcccttggc gtaaccgcct agatcgagct tgaccgccgg
      121 gttgcggctg gcagcctggc tgttttccac cacgatgtcc gccatgcgcg gattggcatt
      181 cacccacttt gctatttcct cggggtcggg atgtaccggc gcgaattcgt cgcgctggaa
      241 gccccacagc tggatcaggt ggccaatggc gggattgaac aggccgccgg attgttcgga
      301 aagtttggca gcatccgcca gcatgccggc cagttccggc gaaaccgcgg ccggttttcc
      361 ggcggcaaat gcgacgttga gccgatccag ctcgctgccc gcctgccagg cgtgcagctg
      421 gcggtgcagg cgctggaact cctgctgcac atggccggaa agttcgcgcg cccgttcctc
      481 ggattcgcca tagatgctga tatccaccag cgtgccgaag acataagcct gcgactgatg
      541 cagcggctcg cgcccgcagc cagtcaggac aagcagtgtt acgagggtag caatccaacc
      601 aatcccaaca cgcttatcca ccttccttat gtgcataatc cttccagcgc atccatgaac
      661 accgccgccg ggtcgcagtc ggtctgggcg gcgatttcca ccatgccggt gggactggtg
      721 acgttaatct cggtgagatg gtcaccgatg acgtcgaggc cgaccaggaa caggccttcc
      781 ttcttgagtt cgatgcccac cgtcgtggca atttcccggt ctcgcaccga gagcggctgc
      841 cccacgccgg taccgccggc ggcgagattg ccgcgcgttt cgccggcctt ggggatgcgc
      901 gccagcgcgt aaggcaaggg ctcgccgtcg ataaccagga tgcgcttgtc gccggccttg
      961 atctccggca ggtagcgctg cgccatgacg gtgcggcgct cgtagtgggt gatggtttcc
     1021 aaaatcacgc cgatgttgtg gtcggcgctg tgcaggcgaa acacgctgct gccgcccatg
     1081 ccgtccagcg gcttgaccac gatatcgcca tgttcggcca gaaaggcacg gatctcggca
     1141 tcgtcgcggg taaccagaaa ttccggcgcg tattgtggaa accgcgccac cgaaagcttt
     1201 tcgttccagt cgcggatggc gcacggccgg ttgaatacac gcgcaccctg cttctcagcc
     1261 agttccagca gataggtgct gtagatgaat tcggtatcga agggcggatc cttgcgcatc
     1321 aggacggcgt cgaactgctc cggcgcgtgt tcggttttgt caccggtttg gtaccaggta
     1381 tcgccatcgt cgaagctgaa aggcacagct ttcaatgcgg cgtgcgggcc gcgcatcatc
     1441 agatcgccct gctctgcgac aaacaggata tggccgcgtc gctccgcttc gcgcatgatg
     1501 gcgacactgg tgtccttgtc agtcttgagg ctgtcgagcg gatcgaggat gaaggccagc
     1561 ttcacggtgc ggtctgttcc agttcaatgg ccgctgccag cagggccaga cgggcgatca
     1621 caccataggc gtagaagcgg ttgggcgccg cgtccggcat gccggcgtgg tcggggagcg
     1681 agcaggcggt atcgaaggcc aggggaacga agtgcatacc cggtgcattc aggttctcgt
     1741 ccatgccgcg tccggtatgc acgcggtaga agccgcccac cacgtagtgg tccatcatgt
     1801 agaccaccgg ctcggccacc gcgtcgttga tgctttcgaa ggtatagacg ccttcctgga
     1861 tgatgacttc ctgcacctgc aggccttcct tgaccaccga catcttgttg cgttgcttgc
     1921 ggttgaggcc gcgcacgtca tccggactct tcaccgtcat gatccccatg ccataggtgc
     1981 cagcatcggc cttgacgatg acgaacggct cctgctcgac accgtattta cgatacttgg
     2041 cgccaatacg cgagagcaga tcggagacct gcgacgccag gcagtcctca ccgacgcggg
     2101 cgtgaaaatc gatcatgccg caactggcga acatggggtc aatcagccac gggtcgatgt
     2161 cgatcagctc ggcgaacttt tgcgccaccc aggtataggc ggaaaaatgg cgcgacttgc
     2221 gccgcaccgc ccaaccggca tgcagcggcg ggatcagcga ttgttccaag tcgcgcagga
     2281 tgtccgggat acccccggaa agatcgttgt tcaacaatac tgcacaagac tcgaagccat
     2341 ccaccaccag ccggttgccc tcgcgccgca ccggttccag ctccagttgc ccgccgtgcg
     2401 gcaagcccag cgcggcggca cggcgcatgg tggaggccga tgcggcgctc cggcgcggcg
     2461 acga
//
LOCUS       DAOMFA010000187         2447 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_6535_length_2447_cov_5.438545, whole genome shotgun sequence.
ACCESSION   DAOMFA010000187 DAOMFA010000000
VERSION     DAOMFA010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 2447)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2447)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2447
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6535_length_2447_cov_5.438545"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..273)
                     /locus_tag="PKW44_08085"
     CDS             complement(<1..273)
                     /locus_tag="PKW44_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020163302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HQR51581.1"
                     /translation="MANELFNDGRHVCVMFSDLVDDSSSQAVQSNQFLIVTDGHGALI
                     DPGGNMTYNALMMAMGKYFHFRQLEYIVASHQDPDIVAPLNRWIIGT"
     gene            complement(378..1016)
                     /gene="thiE"
                     /locus_tag="PKW44_08090"
     CDS             complement(378..1016)
                     /gene="thiE"
                     /locus_tag="PKW44_08090"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HQR51582.1"
                     /translation="MTAKRISGLYAVTPDLADTQQLLAMVEATLQGGASLLQYRNKSA
                     SQHLRHQQAAALLALCRHYNVPLIINDHAGLCCELDADGVHLGGEDGNIAAARALLGT
                     DKIIGASCYNDLDLAVEAKMQGADYVAFGACFSSGTKPAAARAPLELFSRAKHTVGLP
                     TVAIGGIDASNLAQAISAGADSVALISAIFGGTGDICLRTATLARMFHHGRE"
     gene            complement(997..1830)
                     /locus_tag="PKW44_08095"
     CDS             complement(997..1830)
                     /locus_tag="PKW44_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HQR51583.1"
                     /translation="MTQTPPLVLTFAASDPSGGAGMQADLLTLASIGCHPLSVVTAIT
                     VQDTVGVDSVLVFDAEWIDEQARAILEDMQVAAFKLGMLGSVENIAVIAEIVADYPDI
                     PLLIDPILSSGRGDELASDDMQDAMIELLFPQATLITPNSLEARRLAFADDNEETESA
                     SLDDCAAELLQTGSEYVLITGTHERGTEVINTLYGRDGLARAYNWERLPGSYHGSGCT
                     LTSAIAACMAHGLSLEEAVGEAQEYTWQTLKAGFRPGMGQYIPDRLFWAREEEDDGEK
                     N"
     gene            complement(1922..1995)
                     /locus_tag="PKW44_08100"
     tRNA            complement(1922..1995)
                     /locus_tag="PKW44_08100"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1961..1963),aa:Gly,seq:ccc)
     gene            2037..2243
                     /gene="thiS"
                     /locus_tag="PKW44_08105"
     CDS             2037..2243
                     /gene="thiS"
                     /locus_tag="PKW44_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HQR51584.1"
                     /translation="MIALTINGNARSFDATDFTVAELVKKMGLEGKRIAIERNGEIVS
                     RAVFTETRLASGDRLEIVGAVGGG"
     gene            2262..>2447
                     /locus_tag="PKW44_08110"
     CDS             2262..>2447
                     /locus_tag="PKW44_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HQR51585.1"
                     /translation="MDTLNIAGKAYRSRLLVGTGKYQNFEQTRAALDASGCEIVTVAI
                     RRTNIGQNADEPSLLDFV"
BASE COUNT          481 a          667 c          775 g          524 t
ORIGIN      
        1 ggtacctatg atccatctgt taaggggggc gacgatatcc ggatcttgat gcgaagcgac
       61 aatatattcc agttggcgga aatgaaagta cttgcccatc gccatcatca gggcgttgta
      121 ggtcatgttg ccgccggggt cgatcagcgc cccatggccg tcggtgacga ttagaaactg
      181 gttggattgc accgcctggg agctggaatc gtctaccaga tcactgaaca tgacgcaaac
      241 gtgacgcccg tcgttgaata actcgttagc catggatgat tctttctatg cgggtttggt
      301 ggatcaggtc gagttacatg aagcgaggat tgttattgat tgtctcttat agcgcaagcg
      361 ccaaagcggc cacgcgatta ttcgcgacca tgatgaaaca tccgggcgag tgtggctgtt
      421 cgcaagcaaa tatcgccggt tccgccgaat atggcggaga tgagcgcgac actgtcggca
      481 ccggcggaga tggcctgcgc caggttgctg gcatcaatgc caccgatggc gactgtgggc
      541 agaccgacgg tgtgtttggc ccggctgaac agttccagtg gtgcgcgcgc ggcagctggt
      601 ttggtgccgg aactgaagca tgcgccgaag gcaacgtaat cggcgccttg catcttggct
      661 tctactgcaa gatcgaggtc gttgtagcag gaggcgccga tgattttatc cgtgcccagc
      721 aatgcccgtg cggcggcaat attgccatct tcgccgccca gatggacgcc gtctgcatcc
      781 agttcgcagc atagtccggc atggtcgtta atgatcagcg gcacgttgta gtgacggcat
      841 agcgcaagca gggcagcagc ctgttggtgg cggaggtgct gggatgccga cttgttgcgg
      901 tattgcagca gcgaggcccc gccttgcagg gtagcttcga ccatcgccag cagttgttgc
      961 gtgtcggcaa ggtcgggcgt gacggcgtag aggccgctaa ttcttttcgc cgtcatcctc
     1021 ttcttcacgc gcccagaaca ggcggtccgg aatgtattgg cccatgccgg ggcggaagcc
     1081 ggccttgagg gtttgccagg tatattcctg cgcctcgccc actgcctctt caaggctcag
     1141 gccgtgcgcc atgcaggcgg cgatggcaga ggtcagggtg cagccggagc cgtgataact
     1201 gccgggcagg cgttcccagt tgtaagcgcg cgccagacca tccctgccgt acagggtgtt
     1261 gatgacttcc gtcccgcgtt cgtgggtgcc ggtgatgagc acgtactcac tgccggtttg
     1321 cagcaattcc gccgcgcaat cgtccagcga ggcgctctcg gtttcttcgt tgtcgtcggc
     1381 aaaggccagg cggcgcgcct ccaggctgtt gggggtaatc aacgtcgctt gcgggaacag
     1441 cagctcgatc atggcatcct gcatgtcgtc gctggccagc tcgtcgccgc ggccggagga
     1501 caggatgggg tcgatcagta gcgggatgtc cgggtagtca gcaacgattt cggcgatcac
     1561 ggcgatgttt tcgacgctgc ccagcatgcc cagcttgaat gcggccactt gcatgtcttc
     1621 cagaatggca cgcgcctgtt cgtcgatcca ctcggcatcg aataccagca cgctgtctac
     1681 acccacggta tcctgcactg tgatggcggt gactaccgac agcgggtggc agccaatgct
     1741 ggccagggtc agcaggtcag cctgcatgcc ggcgccaccg ctgggatcgc tggcggcgaa
     1801 agtgaggact agcggtgggg tctgggtcat gatcttcagt atttgggttg cggccatttc
     1861 cggccgataa aagcggttgc ctgggtgggc atgcgaacct taaaatggca acctgattgg
     1921 atggagcggg tgacgggaat cgaacccgcg tatcaagctt gggaagctgg cgttctacca
     1981 ttgaactaca cccgcgaaac atgcggattt tacttgattc ggattctttc accaaattga
     2041 ttgctcttac tatcaatggc aacgcccgca gttttgacgc caccgatttc accgtcgcgg
     2101 aactggtgaa aaaaatgggg ctggaaggca agcgcatcgc tattgagcgc aacggcgaaa
     2161 tcgtgtcgcg cgccgtgttt acggaaactc ggctggcttc gggcgacagg ctagagatcg
     2221 tcggcgcggt cggcggtggc taaggaatat tgaaggaaca catggataca ctcaatatcg
     2281 ccggcaaggc ataccgctcg cgcttgctgg taggcaccgg aaaataccag aatttcgaac
     2341 aaacccgtgc ggccctggat gccagcggct gtgaaatcgt gaccgtcgcc atccgccgca
     2401 ccaatattgg ccagaacgcc gacgagcctt cactgctgga tttcgtg
//
LOCUS       DAOMFA010000188         2387 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_6817_length_2387_cov_4.954974, whole genome shotgun sequence.
ACCESSION   DAOMFA010000188 DAOMFA010000000
VERSION     DAOMFA010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 2387)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2387)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2387
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6817_length_2387_cov_4.954974"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..819
                     /locus_tag="PKW44_08115"
     CDS             <1..819
                     /locus_tag="PKW44_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate:AMP phosphotransferase"
                     /protein_id="HQR51586.1"
                     /translation="TVGKYIHEAMRRRLDYDGQTRDPVSPPLLAPVDNLQLINTLDLS
                     KKISKREYEDKLEKYQGKLNLLTREQKFRDISVIAVFEGSDAAGKGGSIHRTTHAVDA
                     RKFSVIPIAAPTDEEQAQPYLWRFWRHLPRRGRLAIFDRSWYGRVLVERVEGFCSEYD
                     WMRAYGEINDFEEQMARKHIVLLKFWLQISKEEQLRRFEERQATAFKRFKITEDDWRN
                     RDKWDAYQMAVCDMIDRTSTTQAPWTVVEANDKSYARIKILKTICDRIEDALGN"
     gene            complement(834..1505)
                     /locus_tag="PKW44_08120"
     CDS             complement(834..1505)
                     /locus_tag="PKW44_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR51587.1"
                     /translation="MTKPNREQDAINAYLNLMEKTGADSANLEIRKQFLQRLVALIGG
                     RPQDFGVYREAVDEALEGLNKAEWPFYISVSREYYRFWIDDIKAIAALHASGGYRATP
                     LVGSVPSEELQVLWNNLDQQSFEVAEKWSLKAYNAALRQEGAEPAVVETRTKLVKLLL
                     VQLRETPERDGYYYQGAVDSLMPLFSMKETRELYIQVVREFFYFWISDPEAVSRIVLH
                     QPADD"
     gene            1631..>2387
                     /locus_tag="PKW44_08125"
     CDS             1631..>2387
                     /locus_tag="PKW44_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HQR51588.1"
                     /translation="MNHDKALAYMHNLLRMMLDKKASDLFISADFPPAMKIDGKMTPI
                     APQKLTGAHTKAFAHALMNEKQRKEFDETKECNFAIWPREIGRFRVNVFVQQENVGMV
                     LRTITTKIPNFDEMGLPSQLKEVMMAKRGLIILVGGTGSGKSTTLAALIDYRNEHSHG
                     HIITIEDPVEYVHPSKNCLITHREVGRDTLDWFPALKNTLRQAPDVILIGEIRDRETM
                     EFALAFAETGHLCMATLHANSANQALDRIINFFP"
BASE COUNT          551 a          746 c          653 g          437 t
ORIGIN      
        1 accgttggca aatatatcca cgaagccatg cgcaggcggc tcgactacga tggacagact
       61 cgggatccgg tctcgccccc cttgcttgca ccagtggaca acctgcagct gatcaatacg
      121 ctggacctgt caaaaaagat cagcaagcgc gaatacgaag acaagctgga aaaatatcag
      181 ggcaagctca acctgctgac gcgcgaacag aagttccgcg acatctccgt tatcgccgta
      241 ttcgagggct cagacgccgc cggcaagggc ggcagcatcc accgcaccac ccatgccgtc
      301 gatgcccgca aattcagcgt catcccgatt gccgccccca ccgacgaaga gcaagcccag
      361 ccttatttgt ggcgattctg gcgccacctg ccgcgccgcg gccgactggc gatcttcgac
      421 cgttcctggt atggccgcgt gctggtggaa cgggtggaag gcttctgctc ggaatacgac
      481 tggatgcgcg cctatggcga aatcaacgat ttcgaggagc agatggcacg caagcacatc
      541 gtgctgctga aattctggct gcaaatcagc aaggaagagc agttgcggcg cttcgaggag
      601 cgccaggcga ccgccttcaa gcgtttcaag atcaccgagg atgactggcg taaccgcgac
      661 aagtgggacg cctaccagat ggccgtgtgc gacatgatag accgcaccag caccacccag
      721 gcgccgtgga ccgtggtcga ggccaacgac aagagctacg cccgcatcaa gatactgaaa
      781 acgatctgcg accgcatcga ggatgcgctg gggaactgac ggggcgatat ccttcagtcg
      841 tcggccggct ggtgcagcac gatccggctg acggcttcgg ggtcgctgat ccagaagtag
      901 aaaaactcgc gtactacctg aatatacaac tcgcgcgtct ccttcatgct gaacagcggc
      961 atcaaggagt ccaccgcgcc ctggtaataa tagccatccc gctcgggtgt ctcgcgcaat
     1021 tgcaccagca gcaacttgac cagcttggtg cgtgtttcca ccaccgccgg ctccgcccct
     1081 tcctggcgca gggccgcgtt gtaggccttc agcgaccact tttccgccac ttcgaatgac
     1141 tgctggtcga ggttgttcca gagcacctgc aactcctcgc tgggaacgct gccgaccagc
     1201 ggggtagcac gataaccgcc gctcgcatgc aatgcggcaa tcgccttgat gtcgtcgatc
     1261 cagaaacggt aatactcgcg ggagacactg atgtaaaacg gccattcggc tttattgagc
     1321 ccttccaaag cctcatccac ggcctcgcgg tagacgccga agtcttgcgg acgaccgccg
     1381 atcagtgcca ccagacgctg caggaattgc ttgcggattt ccagattggc actgtccgcg
     1441 cccgtcttct ccatcaagtt gagataggcg ttgatggcgt cctgctcacg gttcggcttg
     1501 gtcatcggtt atgcgctggc gacaatcgga agttcatttt acagtaggct caaacaagct
     1561 gcataataaa ccgtcgccgg catggcggca gatttctgac tgaataattt ccacatcgac
     1621 aggctatcca atgaaccacg ataaggcatt agcatacatg cacaacctcc tgcgcatgat
     1681 gctggacaag aaggcttcag atctgttcat ctcggcggat tttccgccgg ccatgaagat
     1741 cgacggcaag atgacaccga tcgcgccgca aaaactgacc ggggcacata ccaaggcttt
     1801 tgcccacgca ctgatgaacg aaaaacagcg caaggaattc gatgaaacca aggaatgcaa
     1861 ctttgccatc tggcccaggg agatcggccg cttccgcgtc aacgtcttcg tacagcagga
     1921 aaacgtcggc atggtgttgc gcaccatcac caccaagatc cccaatttcg atgaaatggg
     1981 cctgccgtcg cagttgaaag aggtgatgat ggccaagcgc ggcctcatca tcctggtcgg
     2041 cggcaccggt tccggcaagt ccaccacgct ggcggcgctg atcgattacc gcaacgaaca
     2101 ttcccatggt cacatcatca ccatcgaaga cccggtggaa tacgtccacc ccagcaagaa
     2161 ctgcctgatc acgcaccgcg aagtcgggcg cgacacgctg gactggttcc cggcactgaa
     2221 gaataccctg cgccaggcgc cggacgtgat cctgatcggc gagatccgcg accgcgaaac
     2281 catggaattc gccctcgcct tcgccgaaac cggccacctg tgcatggcga ccctgcacgc
     2341 caacagcgcc aaccaggcgc tggaccgcat catcaacttc ttccccg
//
LOCUS       DAOMFA010000189         2367 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_6915_length_2367_cov_3.721453, whole genome shotgun sequence.
ACCESSION   DAOMFA010000189 DAOMFA010000000
VERSION     DAOMFA010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 2367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2367
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6915_length_2367_cov_3.721453"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..551
                     /locus_tag="PKW44_08130"
     CDS             <1..551
                     /locus_tag="PKW44_08130"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HQR51589.1"
                     /translation="ATALATRRLPECLMETLIPLSRLHADIESRVQAIRADHPQWLCG
                     KGCAGCCRRLADVPQLTATEWDSLREGLATLPAERLREVSRNMAALADQRERPVVCPL
                     LDRSTNACPVYAQRPVACRSYGFYVQRDLGLYCSDIESQVAAGTLADVVWGNHDAIDH
                     RLAGLGEALPLTDWFARWERGK"
     gene            complement(604..981)
                     /locus_tag="PKW44_08135"
     CDS             complement(604..981)
                     /locus_tag="PKW44_08135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR51590.1"
                     /translation="MRKAALILALVTMSANAWSEWVFISESIGDDKSYVDPRSVKKDG
                     NLRRAWVVTDYKGKRKNGVRSFRGLTEVDCKGKRLRILSYTSHSGPMATGDLLGTGKP
                     QPQWARITPGTLGSNLFKYVCQK"
     gene            complement(1190..2062)
                     /locus_tag="PKW44_08140"
     CDS             complement(1190..2062)
                     /locus_tag="PKW44_08140"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP family protein"
                     /protein_id="HQR51591.1"
                     /translation="MAADHSNHPACRDFHAVRSGVQPRRGRPLQPGLTDVTLLVLVLL
                     MAAPSSAAAQTPGSQYLVDGRCVTITEVRNGQVSYAWRAGSASGSGSLPADMLGSRCA
                     AARPPAGHEAPRQEPGEASPAPGAPVAASQAGDTASAGFAQEILQAHNFYRCLHGVPP
                     LQWHAQVADYAQAWVNRGVFEHSDSYHSPLGPMGENLYGSSRLPSGADAARAWYSESA
                     GYAYGSDGSRGSGHFTAMIWKDARYLGCGRARGNISCNYWPGPQARGCAVPNMGGCYA
                     AQVLPPVRNASACR"
     gene            complement(2079..>2367)
                     /locus_tag="PKW44_08145"
     CDS             complement(2079..>2367)
                     /locus_tag="PKW44_08145"
                     /inference="COORDINATES: protein motif:HMM:NF022159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2501 domain-containing protein"
                     /protein_id="HQR51592.1"
                     /translation="GQSLTSGSLGNVAGLLEFCIKNNYLGGNKAASVKDSLMRKLPGG
                     SSSSDSGYNSGAKGILSSSDGSQLDLSAGGLKKQATKQICDKILDQGKSML"
BASE COUNT          399 a          803 c          724 g          441 t
ORIGIN      
        1 ctgccaccgc cctcgcgacc cggcgccttc cggaatgcct catggaaaca ttgatccccc
       61 tgtcccgcct ccatgccgat atcgaatcgc gcgtgcaagc catccgggcg gatcaccccc
      121 aatggctgtg cggcaaaggg tgcgccggtt gctgtcggcg cctcgccgat gtgccgcagc
      181 tgacggcgac ggaatgggac tcgctgcgcg agggcctggc cacgctaccg gcggagcgcc
      241 tgcgggaagt cagccggaac atggccgcct tggccgacca gcgggagcgc cccgtcgtct
      301 gtccgctgct cgaccggtcg accaacgcct gcccggtcta tgcgcagcgg cccgtggcct
      361 gccgcagtta cggcttctac gtgcaacgcg atcttgggct ctattgcagc gacatcgaat
      421 cgcaagtcgc cgccggcacc cttgccgacg tcgtgtgggg caaccacgac gccatcgacc
      481 accggctcgc cggcctcggc gaagcacttc cgctgaccga ctggttcgcg cgttgggagc
      541 gcggcaaata gacgccgcag aaagacaccc tccggggatg tttccaactc tggagcgtcc
      601 agctcacttc tggcagacat acttgaacaa gtttgatccg agcgttcccg gggtgatcct
      661 ggcccactga ggctggggtt tgccggtacc cagcagatcc cctgttgcca tcggcccgga
      721 atgagaggtg tacgaaagga tcctcaaccg cttccccttg caatcgacct cggtcagacc
      781 acggaaagaa cgcacgccat tcttgcgctt ccctttgtag tcagtcacaa cccaagcgcg
      841 gcgcaggttc ccatccttct tgacggacct ggggtcgaca tagctcttgt catcaccgat
      901 gctctcggaa atgaataccc actcggacca tgcgttggcg ctcatggtga caagcgccag
      961 tatcagagcg gctttacgca taccgtccgc ctgcaagtaa ccttctgatt tcagtatcct
     1021 tcggaagtgg attgcctgcc acggacgttc ctgaatcggc tttccccgaa catttctgcc
     1081 ggcctttcgt ttgattggtc atctgacccc acccgccaat tgtatctccg ggattcaatc
     1141 gctgccgcag acgcaccatc cccggcggca cgctgcccgg cgtcgtcggc tacctgcagg
     1201 cgctcgcgtt ccgcacaggt ggcagtacct gtgcggcata gcatccgccc atgttcggca
     1261 ccgcgcaacc tcgtgcctgt ggcccgggcc aatagttgca ggagatgttg cccctggcgc
     1321 ggccgcagcc gagatagcgc gcatccttcc agatcatggc cgtgaagtgg ccgctgccac
     1381 gcgagccgtc ggagccgtag gcgtaacccg ccgattcgct gtaccaggcc ctggctgcgt
     1441 cagcccctga tggcaagcgc gacgaaccgt acagattttc gcccatcggc ccaagcggcg
     1501 agtggtagct gtcactgtgc tcgaacacgc cgcgattcac ccaagcctga gcgtagtctg
     1561 caacctgcgc gtgccattgc agtggtggca cgccatgcag gcaacgatag aaattgtgcg
     1621 cctgcaatat ctcctgcgcg aatccagcgc tggcggtgtc tccggcctgg ctcgccgcta
     1681 cgggcgcgcc cggcgccggg ctggcttcac ctggctcctg gcggggcgcc tcgtgcccgg
     1741 cgggcggccg agcagcggcg cagcgagaac ccagcatgtc cgcaggaagc gagccgctgc
     1801 cgctggcact gccggcgcgc catgcgtagg acacctgccc gttacggacc tcggtgatcg
     1861 tcacgcagcg accatccacc aggtattgcg aaccgggagt ctgggctgcg gcggaggatg
     1921 gcgcagccat cagcagaacc agcacaagta gcgtgacgtc ggtcagtcct ggctgcaggg
     1981 gtctcccgcg ccggggttgg acgccgctgc ggacagcatg gaagtcccgg caagccgggt
     2041 ggttggagtg atccgcagcc atcatccctg ctgcccgctc acagcatgga tttgccctgg
     2101 tcaagaatct tgtcgcagat ctgcttcgtc gcctgtttct tcaagccgcc ggcgctcagg
     2161 tcgagctggc tgccgtcgct gctgctgaga atgcccttgg cgccactgtt gtagccactg
     2221 tcggatgaag atgatccacc cgggagcttg cgcatcagcg agtccttgac cgacgccgcc
     2281 ttgttgccgc cgagatagtt gttcttgatg cagaactcca gcaggccggc gacgttgccg
     2341 agactgcccg aggtgaggga ctgcccc
//
LOCUS       DAOMFA010000190         2342 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_7040_length_2342_cov_4.250109, whole genome shotgun sequence.
ACCESSION   DAOMFA010000190 DAOMFA010000000
VERSION     DAOMFA010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 2342)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2342)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2342
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7040_length_2342_cov_4.250109"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1409)
                     /gene="ahpF"
                     /locus_tag="PKW44_08150"
     CDS             complement(<1..1409)
                     /gene="ahpF"
                     /locus_tag="PKW44_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016668 - oxidoreductase activity, acting
                     on a sulfur group of donors, NAD(P) as acceptor [Evidence
                     IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HQR51593.1"
                     /translation="MLDTNIQNQLKAYFERLEQPIELVASLDGGDKAQEMREMLHEIT
                     ALSSKISLREDGSNSRRPSFSVGRPGEAARVHFAGIPMGHEFTSLVLALLQVSGYPPK
                     VSAEVLEQIKGLDGTFHFETYISLSCHNCPDVVQALNLMAAYNPGIDHVMIDGAMFQA
                     EVNQREIMAVPTVFLNGEIFDQGRMELAQILARIDTQGVKREAEKISAKVPFDVLVVG
                     GGPAGAAAAVYSARKGIRTGVVAERFGGQVMDTLAIENFISVKETDGPKLVAALEEHV
                     RSYDVDIMNLQRADKLVPNGEYIEVKLASGALLKSKSVIIATGARWRELNVPGEKEYR
                     GKGVAYCPHCDGPLFKGKRVAVVGGGNSGVEAAIDLAGFVDHVTLLQFDEQLRADAVL
                     QRKLFSLPNVEIITQAQTPEVTGDGHKANGLSYIDRATGTTHHVELAGVFVQIGLVPN
                     TDWLKDTVALSKYGEIEVD"
     gene            complement(1512..2081)
                     /gene="ahpC"
                     /locus_tag="PKW44_08155"
     CDS             complement(1512..2081)
                     /gene="ahpC"
                     /locus_tag="PKW44_08155"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HQR51594.1"
                     /translation="MATSLINTQVQPFKAQAFHNGQFVEVTEANLKGKWSVVIFMPAA
                     FTFNCPTEVEDAADNYAEFQKLGAEVYIVTTDTHFSHKVWHETSPAVGKAKFPLVGDP
                     THQLTRAFGVHIEEEGLALRGTFVINPEGVIKTLEIHDNAIARDVKESLRKLKAAKYV
                     AEHPGQVCPAKWNEGAATLTPSLDLVGKI"
BASE COUNT          424 a          664 c          705 g          549 t
ORIGIN      
        1 ctgtcaactt cgatttcgcc gtacttgctc agcgcgactg tatctttcag ccagtcggtg
       61 ttcggcacca gaccgatctg cacgaacacc ccggccagtt cgacgtgatg ggtcgttccg
      121 gtggcgcggt cgatgtaact caggccattg gccttgtggc cgtcgccggt gacttccggg
      181 gtctgcgcct gggtaatgat ttccacgttg ggcaggctga acagctttct ctgcaacacg
      241 gcatcggccc gcaactgctc gtcgaactgc agcagggtga cgtgatcgac gaagccggcc
      301 agatcgatgg ccgcttccac cccggaattg ccgccaccaa ccacggcgac ccgcttgccc
      361 ttgaacagcg ggccgtcgca gtgcgggcag taggccacgc ccttgccgcg gtattccttt
      421 tcgccgggta cgttgagttc gcgccagcgc gcaccggtgg cgatgatgac ggacttgctc
      481 ttcagcaatg cgccgctggc gagtttgact tcgatatatt cgccgttcgg caccagtttg
      541 tctgcgcgct gcaggttcat gatgtcgacg tcgtagctgc gcacatgttc ttccagtgcc
      601 gcaacgagtt tgggtccgtc ggtttccttg accgagatga agttctcgat ggccagggtg
      661 tccatcacct gaccgccgaa acgctcggcg accacgccgg tgcgaatgcc cttgcgcgcc
      721 gaatacacgg ctgcggccgc gccggccggg ccgccgccta ccacaagcac atcgaacggc
      781 accttagcac tgattttttc cgcctcgcgc ttgacgccct gggtatcgat gcgcgccagg
      841 atttgggcca gctccatgcg gccctggtcg aatatctcac cattcaggaa taccgtcggt
      901 actgccatga tttcgcgctg gttcacttcc gcctggaaca tcgcgccgtc gatcatgacg
      961 tggtcgatgc cagggttgta ggccgccatc aggttgagcg cctgcaccac atccgggcag
     1021 ttgtggcagg acagagaaat gtaggtttcg aagtggaacg tcccatccag gcccttgatc
     1081 tgttcgagca cttctgcgct caccttgggc ggatagccgc tgacctgcag caatgccagc
     1141 accagcgagg tgaactcgtg acccatcggg atgccggcga aatgcacacg agccgcttcg
     1201 cctgggcgtc cgacgctgaa cgacggccgg cggctgttgc tgccatcttc acgcaggctg
     1261 atcttgcttg agagcgcagt aatttcgtgc agcatctcgc gcatttcctg cgccttgtcg
     1321 ccgccgtcaa ggctggcgac tagctcgatc ggttgttcca atctttcgaa ataggccttg
     1381 agctggttct ggatgttggt gtcgagcatg tagttctcca tgtagggatt tcactgcaaa
     1441 gcgcttgcca gttgagaacc gctttgcgct gaaaaccctc ccgggatatc ccgggaggat
     1501 gtatcaatcg cttagatctt gccgaccaga tccagggacg gagtcagcgt agctgcgcct
     1561 tcgttccact tggccggaca gacttggccc ggatgctcgg ccacatactt ggcggccttg
     1621 agcttgcgca gcgattcctt gacgtcgcgg gcgatggcgt tgtcatggat ttccagcgtc
     1681 ttgattacgc cttccgggtt gatgacgaag gtgccacgca gtgcgaggcc ttcttcttcg
     1741 atgtgcacgc cgaaggcacg ggtcagttga tgggtcggat cgccgaccag cgggaacttc
     1801 gccttgccga cggccgggga ggtctcgtgc cacaccttgt gcgaaaagtg ggtgtcggtg
     1861 gtcacgatgt agacctcggc gcccagtttc tggaattcgg cgtagttgtc ggcggcgtcc
     1921 tcgacttcgg tcgggcagtt gaaggtgaac gcggccggca tgaaaatgac cacggaccac
     1981 ttgcctttca ggttggcttc ggtgacttcg acgaactggc cgttgtggaa agcttgggcc
     2041 ttgaatggtt gaacctgggt attgatcaaa gatgtagcca tgacttgctc cttattggtt
     2101 ggtgagtaaa cagacaacgg agcgcattgt agggaaggat taactatagt tccaattgat
     2161 tgttttcaat ataccgataa atatattcaa tgattgatgc cggtgatgat ctctggccag
     2221 tacccatggg catggtggag gaattgtttt gtatggagtt gtacgaagtg aaaaaaaggg
     2281 ctggttccga tgaaccagcc cttttttgtt tggcgtcccc acggggattc gaaccccggt
     2341 ta
//
LOCUS       DAOMFA010000191         2276 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_7416_length_2276_cov_4.876182, whole genome shotgun sequence.
ACCESSION   DAOMFA010000191 DAOMFA010000000
VERSION     DAOMFA010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 2276)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2276)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2276
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7416_length_2276_cov_4.876182"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..595
                     /locus_tag="PKW44_08160"
     CDS             <1..595
                     /locus_tag="PKW44_08160"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015830108.1"
                     /note="catalyzes the formation of 2-C-methyl-D-erythritol
                     4-phosphate from 1-deoxy-D-xylulose-5-phosphate; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HQR51595.1"
                     /translation="VTTEQALNHPNWVMGPKITIDSATLMNKGLEVIEAHWLFGAEAD
                     RIEVVIHPQSVIHSMVEYVDGSVLAQLGNPDMRTPIAYALGYPERIESGVEALDLFKI
                     GHLDFEQPDMVRFPCLRLAFDALRCGGTAPAILNAANEVAVAAFLQKRLRFIDIPRLI
                     EATLEQVEIAEADSLNDVVDADAAARVAAGEWLERQA"
     gene            592..1947
                     /gene="rseP"
                     /locus_tag="PKW44_08165"
     CDS             592..1947
                     /gene="rseP"
                     /locus_tag="PKW44_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013442259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HQR51596.1"
                     /translation="MTTLLAFLVTLGVLIIVHEYGHYLAARRCGVKVLQFSIGFGRAI
                     YSRHFGRDSTEFIVAAFPLGGYVKMLDEREAPVAEQELPRAFNRQPVWKRMAIVVAGP
                     AANLLLAVLLYWVLFVSGMPGMRPLLGDVPAGSPAAQASMKAEELIVRVGNTAVSTWQ
                     DVRWVLLKESLKAKSVEIEARSGGLETHLHHLDLTQINSEEANRDLLDQLGLAPYRPP
                     LPARVGEVLPDGAAALAGLREGDEILTVNGIAVASWEDFAGLMRQNPGKSVSLKLRRG
                     ADEEQLQLVPEAVEEGGKTIGRIGAGYRMTPEEIDRFMIDVSHAPLPALWQATVKTWD
                     TSVFSLKMLGSMLTGAVSWKGVGGPITIASFAGQSAHAGWKVFLGFLAFISISLGVLN
                     LLPVPVLDGGHLMYYMVEILKGSPVSERAMEIGQRIGFALLGLLMAAAFYNDINRLIT
                     G"
     gene            2020..>2276
                     /locus_tag="PKW44_08170"
     CDS             2020..>2276
                     /locus_tag="PKW44_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HQR51597.1"
                     /translation="MEPFVIKDIRIDGIQRTEAGTVFNYLPVKVGDTMDDDKAAQALK
                     ALYSTGFFKDVRLEAEGDVLLVTVQERPAIAQIGFSGNSSFP"
BASE COUNT          435 a          587 c          753 g          501 t
ORIGIN      
        1 ggtgacgacc gagcaggcgc tgaaccatcc caattgggtg atggggccga aaatcaccat
       61 cgactccgcc accctgatga acaaggggtt ggaggtgatc gaggcgcact ggctatttgg
      121 cgccgaagcc gatcgtatcg aagtggtcat ccatccgcaa agcgtgatcc attccatggt
      181 cgagtacgtg gatggctcgg tgctggcgca gctcggcaac ccggacatgc gcacaccgat
      241 cgcctatgcg ctgggctacc cggagcgcat cgagagcggt gtggaagcgc ttgatctgtt
      301 caagatcggg catctggatt ttgaacaacc tgacatggtg cgcttcccct gcctcaggct
      361 ggcttttgat gcgctgcgct gcggcggcac cgcgccggcg atccttaatg cggctaacga
      421 ggtcgcggta gcggcgttct tgcagaaacg cctgcgattc atcgacattc cacgtctgat
      481 tgaagcgacg ctggagcaag tggaaattgc cgaagctgac agtctgaacg acgtggtgga
      541 tgcggatgct gctgcgcgag tcgctgccgg cgagtggctg gagcggcagg cttgaccacg
      601 ctgctggcct tcctggtgac gctgggcgtg ctgattatag tgcacgaata tgggcattac
      661 cttgccgcca ggcgctgtgg cgtcaaggtg ctgcagttct ccatcggctt tggccgcgcc
      721 atctacagcc ggcacttcgg cagggacagc accgagttta tcgtggccgc ctttcctctc
      781 ggcggctacg tcaaaatgct ggatgagcgc gaggcgccgg ttgcggagca ggaattgcca
      841 cgcgctttca accgtcagcc ggtatggaag cgcatggcga tcgtggttgc cgggccggca
      901 gccaatttgc tgctggctgt tctgttgtac tgggtgttgt tcgtgagcgg gatgcccggc
      961 atgcgcccat tgctgggtga cgtgcctgcc ggcagtccgg cggcgcaggc gagcatgaag
     1021 gcagaggaac tgattgtgcg tgtcggcaac actgcggtaa gcacttggca ggacgtgcgc
     1081 tgggtgttgc tgaaagagtc gctgaaagcc aaaagcgtgg aaattgaagc caggagtggc
     1141 ggcctggaaa cccatctgca tcaccttgac ctcacccaaa tcaattccga agaggccaac
     1201 agggatttgc tcgatcagct tggcctggcc ccttatcggc caccgttgcc ggcacgggtg
     1261 ggtgaggtgt tgccggatgg cgctgcggcg cttgctggtt tgcgcgaggg cgatgaaatt
     1321 ctgacggtca atggcatcgc ggtggcatcg tgggaagatt ttgccgggct gatgcgacag
     1381 aatcccggca agagcgttag ccttaagctg cgtcgcggtg cggatgaaga gcagctgcag
     1441 ctggttccgg aagcggttga agagggcggc aagaccattg gtcgtatcgg tgcaggctat
     1501 cgcatgacgc cagaggaaat cgaccgcttc atgatcgatg tcagtcatgc acctctgccg
     1561 gcgttatggc aggctacggt caaaacctgg gacacttcgg tgttcagcct gaaaatgttg
     1621 ggcagcatgc tgactggcgc cgtatcctgg aaaggggtgg gtggcccgat taccattgcc
     1681 agctttgccg gacagagtgc tcatgcagga tggaaagttt ttctggggtt cctggcgttc
     1741 atcagcatca gcctgggggt gctcaatctg ttgccggttc cggttttgga tgggggccat
     1801 ttaatgtatt atatggtcga gattttgaag ggaagtcctg tttccgagcg tgcgatggaa
     1861 atcgggcaaa gaataggttt tgcgctgctg gggttgttga tggcggcggc tttttataac
     1921 gatatcaacc gcctcatcac tggctgaaaa taggctctaa tgaagttctg gcattttcca
     1981 ttactgctcc tctggcttta tgcggcatca gcgcgggcaa tggaaccctt cgtcatcaag
     2041 gacatccgca ttgacggtat tcagcgtacc gaggcgggga cggtgttcaa ctacctgccg
     2101 gtcaaggtgg gcgacaccat ggacgatgac aaggcggcgc aggcgctcaa ggcgttgtac
     2161 tccaccggct tcttcaagga tgtgcggctg gaagcagagg gggacgtact gttggttacc
     2221 gtgcaggagc gcccggcgat cgcacagatc ggcttcagtg gtaattcctc attccc
//
LOCUS       DAOMFA010000192         2271 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_7439_length_2271_cov_4.904783, whole genome shotgun sequence.
ACCESSION   DAOMFA010000192 DAOMFA010000000
VERSION     DAOMFA010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 2271)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2271)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2271
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7439_length_2271_cov_4.904783"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..351
                     /locus_tag="PKW44_08175"
     CDS             <1..351
                     /locus_tag="PKW44_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HRDC domain-containing protein"
                     /protein_id="HQR51598.1"
                     /translation="ARPVLKGEQQVWLRRDPEPARRKASKTERASRAREAFAGVHDDP
                     LWQALKARRLELAREQGVPPYVIFHDSTLLEILNRKPQTLDELAQVSGVGQAKLAKYG
                     KAFLDALESAENRP"
     gene            348..794
                     /locus_tag="PKW44_08180"
     CDS             348..794
                     /locus_tag="PKW44_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HQR51599.1"
                     /translation="MKIWVDADACPGVIKEILFRAAQRTRIMTTLVANQLLRVPPSPF
                     IRALQVPAGFDVADSRIVQEVQPGDLVITADIPLAAQVIDKQAHALNPRGEFYTPDNM
                     RERLAMRNFMEELRGSGVDISGPSSFNQSDRQAFAAQLDRFLAKPR"
     gene            924..1688
                     /locus_tag="PKW44_08185"
     CDS             924..1688
                     /locus_tag="PKW44_08185"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018507863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HQR51600.1"
                     /translation="MQRLFRIPRMEPEKIRLSKLMSEQGLCSRREADSYIEQGLVFVD
                     GERISELGTRIFPGQKITLAKGAQVQQQARVTILLHKPMGYVSGQAEDGYQPAVTLVN
                     AASRFAEDKVPLRFTPAHLKGLAPAGRLDIDSTGLLVLTQDGRIARLLIGEDSKIEKE
                     YLVRVQGQLSEQGMRQLNHGLSLDGQALKPAKVSWQNEDQLRFVLREGKKRQIRRMCE
                     LVGLKVVGLKRVRIGQIKLADLPPGQWRYLREGERF"
     gene            1756..2166
                     /locus_tag="PKW44_08190"
     CDS             1756..2166
                     /locus_tag="PKW44_08190"
                     /inference="COORDINATES: protein motif:HMM:NF019013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HQR51601.1"
                     /translation="MHNHKEIVRKFYEAIWNNADKDVIPDLICEDFILRGSLGPTHRG
                     HKGFTLYMDFVRGALGNYRCEIIDMISEGDKLYARMLYSGIHRGELFGYAATNGKIRW
                     DGVAVFTFADGKITELWVLGDVLSVMKQLARYVD"
     gene            complement(2180..2266)
                     /locus_tag="PKW44_08195"
     tRNA            complement(2180..2266)
                     /locus_tag="PKW44_08195"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2230..2232),aa:Leu,seq:taa)
BASE COUNT          498 a          711 c          690 g          372 t
ORIGIN      
        1 gcccgcccgg tgctgaaggg cgagcagcag gtctggttgc ggcgcgaccc cgaaccagcc
       61 agacgcaagg ccagcaagac agaacgcgcc tcgcgcgcga gggaagcctt cgccggcgtc
      121 cacgacgacc ccttgtggca ggcgctcaag gccaggcgcc tggaactcgc ccgggaacag
      181 ggcgtgccgc cctacgtcat cttccacgac agcaccctgc tggaaatcct caaccgcaag
      241 ccacaaacgc tggacgaact ggcacaagtc agcggcgtcg gccaggccaa gctggcgaaa
      301 tatggcaagg cattcctcga tgcgctggaa agcgcggaga accggccatg aaaatctggg
      361 tcgatgccga cgcctgcccc ggcgtcatta aagaaatatt attccgtgcc gcacaacgca
      421 cgcggatcat gaccacactg gtcgccaacc agttgctgcg cgtgccaccc tcgcctttca
      481 tccgggcact gcaggtgccc gccggattcg acgtagccga cagccgcatc gtgcaggaag
      541 tacagcccgg cgacctggtc atcactgccg acattccgct ggctgcacag gtgatcgaca
      601 agcaggccca tgcgctgaat ccgcgtgggg agttttatac ccccgacaac atgcgcgagc
      661 gcctcgccat gcgcaacttc atggaagaac tgcgcggcag cggcgtggat atttccgggc
      721 catccagctt caaccagagc gaccgccagg catttgccgc gcaactcgac cggtttctgg
      781 ccaaaccccg ttaggggacg ctgaataaat gagcgaacgg gatgaagtgc aaggcgcacg
      841 gcgcgcagaa cacgagacat atcgagcaga taggcgagtg ggcgagcacc gcgcaacgca
      901 gcaattcgcc cgtgtagcca tttatgcagc gtctctttag aataccgcgc atggaaccgg
      961 aaaagatacg tctttcaaaa ctgatgtccg agcagggact gtgctcgcgg cgcgaagcgg
     1021 acagctatat cgagcaggga ctggtgttcg tcgatggcga acgcatcagc gaactcggca
     1081 cccgcatctt ccccggccag aaaatcaccc tggccaaagg cgcacaggtg caacagcagg
     1141 cgcgcgtcac catcctgctg cacaagccga tgggttacgt ctccggccaa gccgaggacg
     1201 gctaccagcc ggcggtgacg ctggtcaatg ccgcctcgcg ctttgccgaa gacaaggtgc
     1261 cgctgcgttt cacccccgcg cacctcaagg gcctcgcccc ggccggacgg ctggatatcg
     1321 actccaccgg cctgctggtg ctgacgcagg acggccgcat cgccaggctg ctgatcggcg
     1381 aagactcgaa aatcgagaag gaatacctgg tgcgggtgca gggccagctt tcggaacagg
     1441 gcatgcggca gctcaaccac ggcctctccc tcgacggaca ggcgctcaag ccggccaagg
     1501 tcagctggca gaacgaggat cagttgcgct tcgtgctgcg cgaaggcaag aagcgccaga
     1561 tccgccgcat gtgtgaactg gtcggtctga aagtggtcgg acttaaacgg gtgcgcatcg
     1621 ggcaaatcaa gctggccgac ctgccgccgg ggcagtggcg ctatctgcgc gagggcgaaa
     1681 ggttttaatt tcctgttgca tccccccgca attcgtggga aaaagtgtcc atgcgtgtaa
     1741 agatcactgt ccatcatgca caaccataag gaaatcgtcc gcaagttcta cgaagcgatc
     1801 tggaataacg ccgacaagga cgtcattccc gacctgatct gcgaggactt catcctgcgc
     1861 ggctcgctgg gcccgaccca ccgcggccac aaaggcttca cgctatacat ggacttcgtg
     1921 cgcggcgcgc ttggcaatta ccgctgcgaa atcatcgaca tgatctccga aggcgacaag
     1981 ctctacgccc gcatgctgta ttccggcatc caccgcggcg aactgttcgg ctacgccgcg
     2041 accaacggca agatcagatg ggatggcgtg gcggtgttca ctttcgccga cggcaagatc
     2101 acggaactgt gggtactggg cgacgtgctc agcgtgatga agcaactggc gcgctacgtg
     2161 gattgataag tcagggtaat ggtgcccggg accggaatcg aaccggtaca gccgtaaggc
     2221 cgagggattt taagtccctt gtgtctacca attccaccac gcgggctgcc g
//
LOCUS       DAOMFA010000193         2243 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_7589_length_2243_cov_6.419104, whole genome shotgun sequence.
ACCESSION   DAOMFA010000193 DAOMFA010000000
VERSION     DAOMFA010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 2243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2243
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7589_length_2243_cov_6.419104"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            6..521
                     /locus_tag="PKW44_08200"
     CDS             6..521
                     /locus_tag="PKW44_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ANTAR domain-containing protein"
                     /protein_id="HQR51602.1"
                     /translation="MAEAGYTVVAQLSDTADLDRAVGQMQPDVIIIDTESPSRDTLEN
                     ICVVSRDDPRPIVMFTHDGETEQIRAATRAGVSAYVVGGLASGRLKPIMDAAMVRFEE
                     YQALRNELESTTTKLAERKAVERAKGILMKQRGLDEEAAYQALRKMAMERGTRLGEIA
                     RQVIQAAELLS"
     gene            688..1965
                     /locus_tag="PKW44_08205"
     CDS             688..1965
                     /locus_tag="PKW44_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CmpA/NrtA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HQR51603.1"
                     /translation="MKQLNDVSSNVPASPERRKFLSLGAAAASAAAVAGMGSLGISQS
                     AWAAGSDKPELTEVKIGFIPLTDCAPIAVAAEMGFDKKYGIKIIPSKEASWAGIRDKT
                     INGELHAAHVLYGLVYGVQMGIGGPQKDMAVLMTLNNNGQGITLANQLKDKGVTDGRS
                     LKRLIDNENRDYTFAHTFPTGTHAMWLYYWLASFGINPVKDVKTITVPPPQMVANMRI
                     GNMDGYCVGEPWNARAIFDKIGFTVATSQDIWVDHPEKVLGTTAEFMQKNPNTARAML
                     MAVLEACRYIDATANRPAVAKLISGKAYVNAPEEVIEGRFLGHYDNGIGKKWEDPNYM
                     KFFNDGKVPFPYLSDGMWFMTQHKRWGLLKSDPDYLAVAQKVNQLDLYKEAAGALGIS
                     VPKDAMRTSKLIDGMVWEGKDPKAYAAGFKVKA"
     gene            2020..>2243
                     /locus_tag="PKW44_08210"
     CDS             2020..>2243
                     /locus_tag="PKW44_08210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR51604.1"
                     /translation="MSNVIALKLTETNETQPVAKPEAVPSPATGYRRAVAPEWLVTGL
                     QRGIPMLVGVALFIALWALISQQSHNLPGP"
BASE COUNT          515 a          669 c          691 g          368 t
ORIGIN      
        1 aatgcctggc cgaggcaggc tacaccgtag tggcgcagct ttcggatacc gccgacctcg
       61 accgggcggt agggcagatg cagccggatg tcatcatcat cgacaccgaa tcgcccagcc
      121 gcgacacgct ggagaatatc tgcgtagtaa gtcgcgacga tccgcgcccc atcgtgatgt
      181 tcacgcacga cggggaaacg gagcaaatcc gcgcggcgac gcgggccggg gtttccgcct
      241 acgtggtggg cggattagcc agcggcaggc tgaagccgat catggatgcc gccatggtgc
      301 gcttcgagga gtatcaggca ttgcgcaacg agcttgagtc aacgacgacc aagctggcgg
      361 aacgcaaggc tgtggagcgc gccaagggta tattgatgaa acagcgcggc ctcgatgaag
      421 aggccgccta ccaggcgcta cgcaagatgg cgatggaacg cggtaccaga ttgggcgaaa
      481 ttgcccgaca agtgatacag gcagcagaac tactgtcgta agacgaattt agcgggcacg
      541 cccaacgatg ggtatacccg gcagtaacca acagcgcacc gatgcaggtg cgtgcacggg
      601 acaatggcgt ccttctactg gttcctttat caggaattgg cggaaggacg cttttttatt
      661 ggctatcgca attgaaaggg gcttcaaatg aaacagctaa acgacgtgtc aagcaacgtg
      721 ccggccagcc cggagcgacg caaattcctc tccctgggcg cggcggcggc cagcgcggcg
      781 gcggtggcgg gcatgggctc actgggcatc agccagtctg cctgggcggc cggttccgac
      841 aagccggaac tgaccgaagt caagatcggc ttcattccgc tcaccgactg tgctccgatc
      901 gccgtcgccg ccgagatggg cttcgacaag aagtatggca tcaagatcat cccttccaag
      961 gaagcttcct gggccggcat ccgcgacaaa accatcaacg gcgaactgca tgcggcgcat
     1021 gtgctgtacg gcttggtata tggcgtgcag atgggtatcg gtggcccgca gaaggacatg
     1081 gcggtgctga tgacgctcaa caacaacggc cagggcatca ccctggccaa ccagctcaag
     1141 gacaagggcg tcaccgatgg caggagcctg aagcgtctga tcgacaacga aaatcgcgac
     1201 tacaccttcg cccacacctt ccccaccggc acccatgcca tgtggctgta ttactggctg
     1261 gcctcgttcg gcatcaaccc ggtcaaggat gtgaaaacca tcaccgtgcc gccaccgcaa
     1321 atggtggcca acatgcgcat cggcaacatg gacggctact gcgtgggcga gccgtggaac
     1381 gcgcgcgcaa ttttcgacaa gattggcttc acggtggcga cctcgcaaga catctgggtc
     1441 gaccacccgg aaaaggtgct gggcaccacc gccgaattca tgcagaagaa ccccaacacc
     1501 gcgcgcgcca tgctgatggc ggtgctggag gcctgccgct acatcgacgc caccgccaac
     1561 cgtccggcgg tagccaaact gatctccggc aaggcctatg tcaacgcgcc ggaagaagtg
     1621 atcgaaggtc gcttcctcgg ccactacgac aacggcatcg gcaaaaagtg ggaagacccc
     1681 aattacatga aattcttcaa cgacggcaag gtgcccttcc cttacctctc ggacggcatg
     1741 tggttcatga cccagcacaa gcgctggggc ctgctcaaga gtgacccgga ttacttggcc
     1801 gtggcacaga aggtcaacca gctcgatctg tacaaggaag ccgccggcgc gctgggtatc
     1861 agcgtaccca aggacgccat gcgtacatcg aaactgatcg acggcatggt gtgggagggc
     1921 aaggacccca aggcctacgc cgccgggttc aaggtcaaag catagcgacg tagtgcaggc
     1981 ggcctcggct gcactaccag taaaccagga gagtaaaaca tgagcaacgt cattgcattg
     2041 aaactgacgg aaactaacga aacgcagccc gtcgcaaagc cggaagccgt gccatcacct
     2101 gcgacaggct accgccgcgc ggtggcaccg gaatggctgg tcacgggttt gcagcgcggg
     2161 attcccatgc tggtcggggt ggcgctgttc atcgcgctgt gggcgctgat ttcgcaacag
     2221 tcgcataacc tgccggggcc cga
//
LOCUS       DAOMFA010000194         2230 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_7650_length_2230_cov_5.651034, whole genome shotgun sequence.
ACCESSION   DAOMFA010000194 DAOMFA010000000
VERSION     DAOMFA010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 2230)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2230)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2230
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7650_length_2230_cov_5.651034"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1151
                     /locus_tag="PKW44_08215"
     CDS             <1..1151
                     /locus_tag="PKW44_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013964445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HQR51605.1"
                     /translation="IGKVYAGRNGSVGAITEDLIDTSLESDAAIAALRSTPSGAFGSC
                     RYKLKDLETNRREYERLIEVFKAHNIGYFFYNGGGDSADTCYKVSQLSEKMGYPVQAI
                     HVPKTVDNDLPITDCCPGFGSVAKYIAVSTLEASFDVRSMAKTSTRIFVLEVMGRHAG
                     WIAAAGGLVEDYSIPVVILFPEVEFDQEKFLARVDAKVKKHGYCTVVVSEGCHYPDGR
                     FIADQGIKDVFGHAQLGGAAPVVANMIKDALGYRYHWAVADYLQRAARHIASATDVRH
                     AYELGKKAVKLALKGRNSVMPTVIRVADAPYEYRIGYAELKDVANVEKFMPRDFISKD
                     GYGITPKCKRYLRPLIQGEDYPAYEAGLPRYVTLRNAAVAKKLPKFEL"
     gene            1345..>2230
                     /locus_tag="PKW44_08220"
     CDS             1345..>2230
                     /locus_tag="PKW44_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HQR51606.1"
                     /translation="MDQALLLALGCSVLALFYGGVTIAWVLAKPTGNDHMRAIAAAIQ
                     EGASAYLNRQYTTIGLVGALLFAVIWLGLGSLTAVGFAIGALLSAAAGYIGMNISVRS
                     NVRTAQAAHDGLDAALQVAFRGGAITGMLVVGLGLLGVAGYFAFLLANTAEGATVNIS
                     PLVGLAFGSSLISIFARLGGGIFTKGADVGADLVGKVEAGIPEDDPRNPAVIADNVGD
                     NVGDCAGMAADLFETYAVTIIATMLLGALLMPGSANALTYPLVLGGISIVASIIGTFF
                     VKAREGGKIMNALYRGIIV"
BASE COUNT          390 a          706 c          697 g          437 t
ORIGIN      
        1 agattggcaa agtctatgcc gggcgcaacg gcagcgtcgg cgcgattacc gaggacctga
       61 tcgacaccag tctggagtcc gacgctgcca tcgccgcgct gcgctccact ccttccggcg
      121 ccttcggctc ctgccgatat aaattgaaag acttggaaac caaccggcgc gaatacgaac
      181 gcctgatcga ggtgttcaag gcgcacaaca tcggctactt cttctacaac ggcggcggcg
      241 attccgccga cacctgctac aaggtgtcgc agctttcgga aaaaatgggc tacccggtgc
      301 aggccatcca cgtacccaag accgtggaca acgacctgcc gatcaccgac tgctgccccg
      361 gcttcggtag cgtcgccaag tacatcgccg tgtccacgct ggaggcctcg ttcgacgtgc
      421 gctccatggc caagacctcg accagaatct tcgtgctgga ggtgatgggc cgccatgccg
      481 gctggatcgc cgcggccggt gggctggtgg aggactacag catcccggtg gtgatcctgt
      541 tccccgaagt ggagtttgac caggaaaaat tcctggcgcg ggtggatgcc aaggtgaaga
      601 agcacggcta ctgcaccgtg gtggtctccg agggctgcca ctacccggac ggccgcttca
      661 tcgccgacca gggcatcaag gacgtcttcg gccacgccca gttgggtggc gcggcgccgg
      721 tggtggccaa catgatcaag gacgcgctgg gttaccgcta ccactgggcc gtggccgact
      781 acctgcagcg cgccgcgcgc cacatcgctt ccgccaccga cgtcaggcat gcctacgaac
      841 tgggcaagaa agcggtcaaa ctcgcactca aaggcaggaa ttcggtcatg cccaccgtga
      901 tccgcgtagc cgatgcgcct tacgaatacc gcatcggcta cgccgagttg aaggacgttg
      961 ccaatgtcga gaaattcatg ccgcgcgatt tcatcagcaa ggatggctac ggcatcacgc
     1021 cgaagtgcaa gcgctacctg cgtccgctga tccagggcga ggattatccc gcctacgagg
     1081 ccggcctgcc ccgctatgtc acgctgcgca atgcggcggt ggcgaaaaaa ctgccaaagt
     1141 tcgagcttta aactccccgt acatcgtcca tctgcctgct tgctaccccg cgctcccctt
     1201 gcgcgggagg gcggaagcgc aggcctggtc aggcgcttcc gcagcaccgg attatggggt
     1261 aagcttcgcc cacggaggct gggggcgttt gcatcatgac ccctgcccta tttctaattt
     1321 gttgcgtttg ggaggggtgt ttcgatggat caagcattat tgctggcgct cggatgttcc
     1381 gtgctggcgc tgttctacgg tggtgtcacg attgcatggg tgctggccaa gccgaccggc
     1441 aacgaccata tgcgcgcaat cgcagcagcg atccaggaag gcgcctctgc ttatctcaac
     1501 cggcagtaca ccaccatcgg gctggtcggt gcactgctgt tcgcggtgat ctggcttggg
     1561 ctgggcagcc tgactgccgt tggtttcgcc atcggcgcgc tgctttccgc cgctgccggc
     1621 tacatcggca tgaacatttc ggtacgctcc aacgtgcgca ccgcgcaggc ggcgcatgac
     1681 ggcctggatg ctgccttgca ggtggccttc cgcggcggcg ccattaccgg catgctggtg
     1741 gtgggcttgg gcctgctggg cgtggcgggc tactttgcct tcctgcttgc caatacggca
     1801 gagggcgcga cggtcaacat ttccccgctg gtgggcctgg ccttcggcag ttcgctgatc
     1861 tccatttttg cgcgtctggg cggcggcatc ttcactaagg gtgcagacgt cggtgccgac
     1921 ctggtgggca aggtggaagc cggcatcccc gaggatgacc cgcgcaaccc ggcggtgatc
     1981 gccgacaacg tcggcgacaa cgtcggcgac tgtgccggca tggctgccga cctgttcgag
     2041 acctatgccg tcaccatcat cgccaccatg ctgttgggcg cgctgctgat gccgggctcg
     2101 gctaacgcac tgacctatcc gctggtgctg ggtggcatct ccatcgttgc ttccatcatc
     2161 ggcaccttct tcgtcaaggc gcgcgaaggc ggcaagatca tgaacgcgct gtaccgcggc
     2221 atcatcgtct
//
LOCUS       DAOMFA010000195         2132 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_8239_length_2132_cov_5.170438, whole genome shotgun sequence.
ACCESSION   DAOMFA010000195 DAOMFA010000000
VERSION     DAOMFA010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 2132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2132
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8239_length_2132_cov_5.170438"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..756)
                     /locus_tag="PKW44_08225"
     CDS             complement(<1..756)
                     /locus_tag="PKW44_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013441703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HQR51607.1"
                     /translation="MQPILKSSKLNNVCYDIRGPVLARAREMEDEGHRIIKLNIGNPA
                     PFGFEAPEEIVQDVIHNLRDASGYSDSKGLFAARRAIVHYTQQKNIPGVTTDDVIIGN
                     GVSELIVMAMNALLNNGDQVLVPAPDYPLWTAAVSLSGGTPRHYLCDEASDWLPDLRD
                     IENKINANTRGIVIINPNNPTGALYPREILEGIIEIARHHGLIVFADEIYDKVLYDGN
                     EHVSIASLADDVLFVTFNGLSKNYRSCGYRAGWM"
     gene            815..1183
                     /locus_tag="PKW44_08230"
     CDS             815..1183
                     /locus_tag="PKW44_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020168751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mth938-like domain-containing protein"
                     /protein_id="HQR51608.1"
                     /translation="MKLHLTQSAGNHLITGYGTGWVEINASRYGQSLVVLPALLVTDW
                     QVEDFDSLAETHFERLASLAPEVVLLGTGATHRFARPRLYRALTEANIGLECMDTAAA
                     CRTYNILMAEGRHVAAALIV"
     gene            1354..1908
                     /locus_tag="PKW44_08235"
     CDS             1354..1908
                     /locus_tag="PKW44_08235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR51609.1"
                     /translation="MSLVLFLVYWVGVPLLIVLVARNLWRQSRTPVHKGLVVAASAMI
                     LLGLLWLAEGEKWLLDRQVRELCAKDGGVKVYETAKLPPEKFDQWGNVGILDKNHAKP
                     TDEYYYESEEHYYQQGNPALLRSRGWIVRRSDGKVLGESIRYGRGGGDLPGPWHGTSF
                     HCPPIAAKGMPNLETSIFLKGDEK"
     gene            1905..>2132
                     /locus_tag="PKW44_08240"
     CDS             1905..>2132
                     /locus_tag="PKW44_08240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR51610.1"
                     /translation="MNSIETYFEQAELALAAYSDLAPGMIFDDYKAALMDGGKGMSPT
                     QAADFASKWTVVTQYTDPITGVSATVFQEGVN"
BASE COUNT          437 a          600 c          654 g          441 t
ORIGIN      
        1 catccagccg gcgcggtagc cgcaggagcg gtagttcttg gacaggccgt tgaaggtgac
       61 gaacagcacg tcatcggcca gcgaggcgat ggaaacatgc tcattgccgt cgtacagcac
      121 cttgtcgtag atctcgtcgg cgaacacgat cagcccatgg tggcgggcga tctcgatgat
      181 gccctccagg atctcgcgcg gatacagcgc tccggtcggg ttgttggggt tgatgatgac
      241 gatgccgcgc gtgttggcgt tgatcttgtt ctcgatatcc ctgaggtcgg gcagccagtc
      301 gctggcctcg tcgcacaggt agtggcgcgg cgtaccgccg gaaaggctca ctgcagccgt
      361 ccacagcggg tagtccggcg ccggcaccag cacctggtcg ccgttgttga gcagcgcgtt
      421 catcgccatc acgatcagct cggacacgcc gttgccgatg atgacgtcgt cggtggtcac
      481 gccggggatg ttcttctgct gcgtgtaatg cacgatggcg cggcgcgcgg cgaacaggcc
      541 cttggaatcg ctgtagccgg aggcgtcgcg caggttgtgg atcacgtcct gcacgatctc
      601 ttccggcgcc tcgaagccga acggcgccgg gttgccgatg ttcagcttga tgatgcggtg
      661 gccctcgtcc tccatttcgc gcgcgcgcgc cagcaccggc ccgcggatgt catagcagac
      721 gttgttgagc ttggatgatt tcagaatggg ctgcacggcg gtcaagggct tctgtaaaat
      781 gcgtaatgtt accttgtccc accccccaca ggcaatgaaa ctgcacctca cccaatcggc
      841 aggcaaccac ctcatcaccg gctacggcac gggctgggtg gaaatcaacg ccagtcgcta
      901 cgggcaaagc ctggtcgtgc tgcccgccct gctggtcacc gactggcaag tcgaagattt
      961 cgacagcctg gccgaaaccc atttcgagcg gcttgcatcg ctcgccccgg aagtcgtgct
     1021 gctcggcacc ggcgccacgc accgcttcgc ccggccgcgc ctctaccgtg ccctcaccga
     1081 ggccaacatc ggactggaat gcatggacac tgccgccgcc tgccgcacct acaacatcct
     1141 gatggcggaa ggccgtcacg tggccgccgc gctgattgtt tgaaaagcgc aggatcaggt
     1201 ctgatgacga tgccaaacag cagcaggagg tcatcggtca atgacgacga cctcatttat
     1261 tgagctcgtg gatcacgatg atggcagcgg ccctctcgtc gacggtatgg aaaaccgggt
     1321 ggctttgaat aatctcgaat ggaaagtgac agcatgagcc tcgtactgtt tcttgtttac
     1381 tgggtaggcg ttccgcttct catcgtgctt gtggcgcgca acttgtggcg ccaatcaaga
     1441 acgccggttc acaaagggtt ggtcgtggcc gcaagtgcga tgatcttgct gggtctgctg
     1501 tggctggcag aaggggagaa atggctgctc gaccggcagg tgcgcgagct atgtgccaag
     1561 gatggcgggg tcaaggtgta tgagacggcg aagctgccgc cagaaaaatt tgaccaatgg
     1621 ggaaatgtag ggattcttga caaaaatcac gctaaaccaa cagacgagta ttactacgaa
     1681 tctgaagagc actattacca acaaggcaat ccagcattgc tacggagtcg cggctggatt
     1741 gttcggcgca gtgacgggaa ggttctgggg gagtcgataa ggtacggacg cggtggaggc
     1801 gatcttcctg gcccgtggca tggaacatcg tttcattgtc cgccgatagc tgccaaagga
     1861 atgccgaatc ttgaaacgtc aatatttctg aaaggtgatg agaaatgaat agcatcgaaa
     1921 cctattttga acaagccgaa ctcgcccttg ctgcgtattc tgatcttgca cctgggatga
     1981 tttttgacga ttacaaggct gcattgatgg atggtgggaa aggcatgtcc cccacccaag
     2041 ccgctgactt cgcctcgaaa tggactgttg tcacccaata caccgacccg attaccggcg
     2101 tctccgccac cgtctttcaa gagggggtta ac
//
LOCUS       DAOMFA010000196         2008 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_9163_length_2008_cov_5.513057, whole genome shotgun sequence.
ACCESSION   DAOMFA010000196 DAOMFA010000000
VERSION     DAOMFA010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 2008)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2008)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2008
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9163_length_2008_cov_5.513057"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..493)
                     /gene="eno"
                     /locus_tag="PKW44_08245"
     CDS             complement(<1..493)
                     /gene="eno"
                     /locus_tag="PKW44_08245"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011001079.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     2-phospho-D-glycerate in glycolysis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HQR51611.1"
                     /translation="MSAIVEVIAREILDSRGNPTVEADVLLESGIIGRAAVPSGASTG
                     SKEAVELRDDDKDRYLGKGVLRAVENVNTEITEAIIGLDAEEQSFIDNTLIELDGTEN
                     KSRLGANAILAVSLACARAAAEESGLPLYRYLGGAGRMQLPVAMMNIITGGAHASNSI
                     DMQE"
     gene            complement(610..1452)
                     /gene="kdsA"
                     /locus_tag="PKW44_08250"
     CDS             complement(610..1452)
                     /gene="kdsA"
                     /locus_tag="PKW44_08250"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HQR51612.1"
                     /translation="MKLCGFEVGLDQPLFLIAGPCVIESQQMALDTAGQLKEICAELG
                     VNFIYKSSYDKANRSSGKTFRGLGMHEGLRILSEVRKQIGVPVLTDVHTEQEVPEVAA
                     VVDVLQTPAFLCRQTDFIEACARSGKPVNIKKGQFMAPWDMKNVVDKAKAVNGGADNI
                     MVCERGVSFGYNTLVSDMRGLAVMRNTGCPVVFDATHSVQQPGGQGDKSGGQREHVPV
                     LARAAVASGVAGVFMETHPNPAEALSDGPNAWPLPKMKALLETLVEIDRLVKKRGFIE
                     NELN"
     gene            complement(1449..>2008)
                     /gene="pyrG"
                     /locus_tag="PKW44_08255"
     CDS             complement(1449..>2008)
                     /gene="pyrG"
                     /locus_tag="PKW44_08255"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019882041.1"
                     /note="CTP synthase; cytidine triphosphate synthetase;
                     catalyzes the ATP-dependent amination of UTP to CTP with
                     either L-glutamine or ammonia as the source of nitrogen;
                     in Escherichia coli this enzyme forms a homotetramer;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CTP synthetase"
                     /protein_id="HQR51613.1"
                     /translation="AIRYAREHKIPYLGICLGMQLAVIEFARDVAGLANANSTEFDPD
                     GPHPVVGLITEWKDASGNVEKRDENSDLGGTMRLGAQKCPIVPGTLAARIYGAEVNER
                     HRHRYEVNNHYVDQLKLAGLVVSARTPSEDLCEIVELSQDVHPWFVACQFHPEFTSNP
                     RAGHPLFKAYVEAAIQRKEGKEGKK"
BASE COUNT          389 a          615 c          574 g          430 t
ORIGIN      
        1 tttcctgcat atcgatgctg ttggaagcat gtgcaccacc agttatgata ttcatcatcg
       61 ccaccggcag ttgcatgcga ccggcaccgc ccagataacg gtatagcggc agaccggatt
      121 cctcagcagc ggcgcgggcg caggccaggg acaccgccag tatggcattg gcgccgagac
      181 ggctcttgtt ttcagtgccg tccagctcga tcagggtgtt gtcgatgaag ctttgctcct
      241 cggcatccag cccgatgatg gcttcggtga tttcggtatt gacgttttcc actgcacgca
      301 gcacgccctt gcccagatag cgatctttat cgtcgtcacg cagctcgacg gcttccttgc
      361 tgccggtgga agcgccggac ggcacagcgg cacgaccaat gatgccggat tccagcagaa
      421 cgtcggcttc gacagtagga ttgccgcgcg aatccagaat ttcacgggca atgacttcta
      481 cgatggcact catttatttt ccttttcgaa ggcttttaat tcgcggatgg gcatagaaag
      541 aaatatgtcg acaggccccg acaacttcat tcaagcaagt tcctcgccat aaatgcttta
      601 catcagcggt taattcaatt cgttttcaat aaaaccgcgc ttctttacca ggcgatcgat
      661 ctcgaccagc gtttccagca gcgctttcat cttcggcaac ggccaggcat tggggccatc
      721 ggacaaggct tcagccggat tcggatgcgt ttccatgaac actcctgcca cgccgctggc
      781 caccgccgca cgcgccagca ccggcacatg ctcgcgctgg ccgccgctct tgtcgccctg
      841 accgccgggc tgctgcaccg aatgggtggc atcgaacacc accgggcacc cggtgttgcg
      901 catcacggcc aggccgcgca tgtcggacac cagcgtgttg tagccgaagg aaacaccacg
      961 ctcgcacacc atgatgttgt cggcgccacc gttgaccgcc ttggccttgt cgacaacgtt
     1021 cttcatgtcc cacggcgcca tgaactggcc cttcttgatg ttcaccggct tgccggaacg
     1081 cgcgcaggcc tcgatgaagt cggtctggcg gcacaggaag gccggggttt gcagcacgtc
     1141 gaccacggcg gccacttccg gcacttcctg ctcggtatga acatcggtca gcaccggcac
     1201 gccgatttgc ttcctgacct cggacaaaat gcgcaggccc tcatgcatac ccaggccgcg
     1261 gaaggtcttg ccggaactgc ggttggcctt gtcgtaggac gacttgtaga tgaagttgac
     1321 gccaagctcg gcacagatct ccttcaactg gccggccgta tccagcgcca tctgttgcga
     1381 ctcgatcacg caggggccgg caatcaggaa cagcggctgg tccaggccga cttcaaaacc
     1441 gcacagtttc atttcttgcc ttccttgcct tctttacgtt ggatcgcggc ttccacatag
     1501 gccttgaaca gcggatgccc ggcgcggggg ttggaggtga attccgggtg gaactggcag
     1561 gcgacgaacc acgggtgaac atcctgcgac agttcgacga tctcgcacag gtcctcgctg
     1621 ggagtccggg cagaaaccac caggccggcc agtttgagct ggtctacgta atggttgttc
     1681 acttcatagc ggtgacggtg gcgctcgttg acctctgccc cgtaaatccg tgctgccaat
     1741 gtgccaggga cgatgggaca cttttgcgcg cccaggcgca tggtgccacc aaggtcggag
     1801 ttttcgtcgc gcttttcaac gttgccggag gcatccttcc attcggtaat gaggccgacc
     1861 accggatgcg ggccatcagg atcgaattcg gtgctattgg cattggccag cccggccacg
     1921 tcgcgcgcaa actcgatcac ggctagctgc atgccgagac aaataccgag gtaaggaatc
     1981 ttgtgttcgc gtgcatagcg gatcgcgg
//
LOCUS       DAOMFA010000197         1999 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_9248_length_1999_cov_5.190844, whole genome shotgun sequence.
ACCESSION   DAOMFA010000197 DAOMFA010000000
VERSION     DAOMFA010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 1999)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1999)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1999
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9248_length_1999_cov_5.190844"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..919
                     /gene="infB"
                     /locus_tag="PKW44_08260"
     CDS             <1..919
                     /gene="infB"
                     /locus_tag="PKW44_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HQR51614.1"
                     /translation="LVQSGTLKRGDMLLAGSSFGRVRAMLDENGVDVKEAGPSIPVEI
                     LGLSDVPNAGEETIVLNDERKAREIALFRQGKFRDVKLARQQAAKLENMFEQMGEGEV
                     KSLPLIIKSDVQGSYEALATSLQKLSTSEVKVNILHTGVGAITESDINLASASKAVVI
                     GFNVRADGGARKLAESAGVDIRYYNIIYEAVDEVKAALGGMLSPEKKENAIGLVEIRE
                     VFRISKVGAVAGCYVLEGLIKRGSSVRVLRDNVVVHEGELDSLKRFKDDVREVKAGFE
                     CGLSLKNFNDIQVGDQLEAFEVVEVARTL"
     gene            919..1281
                     /gene="rbfA"
                     /locus_tag="PKW44_08265"
     CDS             919..1281
                     /gene="rbfA"
                     /locus_tag="PKW44_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HQR51615.1"
                     /translation="MAKEFARSQRVAEQIQRELADLLQFEIKDPRVSMVTITEVEVTG
                     DLAHAKVYFSAPENPPELMQGLLKSAGYLRSQLSQRMLMRTVPQLHFVYDASIERGMK
                     LSRLIDDAVAQDKNHPQD"
     gene            1302..>1999
                     /gene="truB"
                     /locus_tag="PKW44_08270"
     CDS             1302..>1999
                     /gene="truB"
                     /locus_tag="PKW44_08270"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020167071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HQR51616.1"
                     /translation="MQYKRIKRPVDGVLLLDKPVGLSSNQALQQAKCLYQAAKAGHTG
                     SLDPFATGLLPICLGEATKFSHFLLDADKTYCATMRLGATTTTGDTEGEIVSRAEVSV
                     TNEQMDDVLRRFSGVISQVPPMYSALKHQGKALYEYARAGMEIERPARTVTIHRIVLN
                     TLTGSSAEITVSCSKGTYIRTLAEDIGNALGCGAYLTQLRRLQSGYFDLAQAVTLEQL
                     EAMSMEQRESCLLPS"
BASE COUNT          429 a          544 c          625 g          401 t
ORIGIN      
        1 cctggtgcaa tccggcacgc tcaagcgtgg cgatatgctg cttgccggca gttcgttcgg
       61 tcgcgtgcgc gccatgctgg atgaaaacgg tgtggacgtg aaggaagcag ggccgtcgat
      121 cccggtagaa attcttggtc tgtctgatgt acccaatgcc ggcgaagaga ctatcgtgct
      181 caacgacgag cgcaaggcgc gcgaaattgc gctgttccgc cagggaaagt tccgcgacgt
      241 caaactggcc agacagcagg cagccaagct cgaaaacatg ttcgagcaga tgggcgaagg
      301 cgaggtcaag agcctgccgc tgattatcaa atcggatgtg caaggttcct acgaggccct
      361 tgccacttct ttgcagaagc tgtccaccag cgaagtcaag gttaatattc tgcacaccgg
      421 cgtgggcgcg atcaccgaat ccgacatcaa tctggcctcc gcatccaagg ccgttgtcat
      481 cggcttcaac gtgcgcgctg atggcggtgc gcgcaagctg gccgaatccg caggtgtgga
      541 tattcgctac tacaacatca tttacgaagc cgtggatgag gtgaaagcgg cgctgggtgg
      601 catgctgtcg ccggagaaga aggaaaacgc cattggtctg gtggaaatcc gcgaggtatt
      661 ccgtatctct aaggtcggag cagtggctgg ttgctacgtg ctggagggtc tgatcaagcg
      721 cggctcctcg gtgcgtgtgc tgcgtgacaa cgtggtggtg catgaaggtg agctggattc
      781 gctcaaacgc ttcaaggatg atgtgcgcga ggttaaggcg ggcttcgagt gcggactgtc
      841 gctcaagaac ttcaacgaca tccaggtggg cgaccaactg gaagcgtttg aagtggtgga
      901 agtagcacgt acgctctaat ggccaaggaa tttgccagaa gtcagcgtgt tgccgagcag
      961 atccagcgcg aactggcaga tctgctgcaa ttcgagatca aggatccgcg tgtgtccatg
     1021 gtcactatca ccgaggttga ggtgacaggt gatctggcgc acgctaaggt ctatttcagc
     1081 gcccctgaaa atccgcctga gttgatgcag gggctgctga agtcagccgg ctacctgcgc
     1141 agccagcttt cccagcgcat gctgatgcgt acggtgccgc agctgcactt tgtctatgac
     1201 gcctccatcg aacgcggcat gaagctgtcc cgcctgatcg acgatgcggt ggcgcaagac
     1261 aaaaaccacc cgcaagactg attcctcaag attattccat ggtgcagtac aagcgcatca
     1321 agcgtccggt ggacggcgtg ctgttgctgg acaagcctgt cgggctatcc tccaaccagg
     1381 cgttgcagca ggctaagtgc ctgtatcagg cggccaaggc cggccacact ggcagcttgg
     1441 atccgtttgc taccggcctg cttcctatct gcttgggcga ggcgaccaag ttttctcatt
     1501 tcctgctgga cgccgacaaa acctactgtg ccacgatgcg gctgggggcg actacgacca
     1561 ccggcgacac cgaaggcgaa attgtctcgc gtgccgaagt cagtgtgaca aacgagcaaa
     1621 tggatgacgt actgcgccgc ttctctggag tgatcagcca ggtgccgccc atgtattccg
     1681 cgctcaagca ccagggtaag gccttgtatg aatacgcgcg cgctggcatg gaaatcgaac
     1741 ggccggcgcg cacggtgacg atacatcgca tcgtgctaaa taccctaacg ggtagcagcg
     1801 ccgagatcac cgtgtcatgc agcaaaggga cctacatccg cacactggcc gaggatattg
     1861 gcaatgcctt gggctgcggg gcctacctga cgcaactgcg ccggctgcag agcggttatt
     1921 tcgatctggc gcaggcggtg acgttggagc agctggaagc tatgagcatg gagcagcgcg
     1981 agtcctgcct gctgccgtc
//
LOCUS       DAOMFA010000198         1924 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_9915_length_1924_cov_5.867844, whole genome shotgun sequence.
ACCESSION   DAOMFA010000198 DAOMFA010000000
VERSION     DAOMFA010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 1924)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1924)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1924
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9915_length_1924_cov_5.867844"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            112..480
                     /locus_tag="PKW44_08275"
     CDS             112..480
                     /locus_tag="PKW44_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012777507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MliC family protein"
                     /protein_id="HQR51617.1"
                     /translation="MKKRFFYLTLFYPVGLALLLPGCGGGKLWPFDGDGATTSSRKPA
                     NATEYQCDGGKRFYVRMMDNGATAWLILPDREVALAKASSGSRYSNGIAVLEINGSEA
                     ALADGTVTYSGCKVPAPAAK"
     gene            complement(484..1260)
                     /locus_tag="PKW44_08280"
     CDS             complement(484..1260)
                     /locus_tag="PKW44_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HQR51618.1"
                     /translation="MLRIISANLNGIRSASSKGFYDWLQQQNADLLCMQELKAQAPDM
                     SRHMLQPDDWFGYFHYAEKKGYSGVGIYSRKQPDAIIEGLGMPDIDAEGRYIEARFGN
                     LSVVSLYLPSGSSGEERQAVKFSVMERFYPHLAALKASGREVVVCGDWNIAHQEADLK
                     NWRGNRKNSGFLPEERAWMTRIFNELGWVDVYRRLHPDTTDECYTWWSNRGQAWAKNV
                     GWRIDYQIATPGIAAKALSTSVYKDERFSDHAPLTVDYDV"
     gene            1284..>1924
                     /gene="pyrE"
                     /locus_tag="PKW44_08285"
     CDS             1284..>1924
                     /gene="pyrE"
                     /locus_tag="PKW44_08285"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013965299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HQR51619.1"
                     /translation="MTDFRQQFIAFAIDKQVLRFGEFITKAGRKTPYFFNTGLFNDGD
                     SLRKLGQFYAKAILESGLEFDVLFGPAYKGIPLAASIAIALSEAGRNVPFSFNRKEAK
                     DHGEGGTIVGAPLKGKVLIVDDVISAGTSIRESVEIIRVNGAAPCGVFIALDRQERGS
                     GELSAVQEVERSYGIPVFGIASLAILIEYLRNQPHMAHNLDAIERYQSEYGIAS"
BASE COUNT          384 a          546 c          596 g          398 t
ORIGIN      
        1 ggggaagtcc tgcaagccat ggcgctcaag ctttgcgaag atggcaggca ttgaagcgcg
       61 cggccgacgg tggcaatatg cgcctttgcc aaatattgga agacgacctt tgtgaaaaaa
      121 agattttttt acctgacgct gttctacccg gttggcttgg ccctgttgct gccgggctgt
      181 ggagggggaa aattgtggcc gttcgatggg gatggtgcga cgacatcatc cagaaagccg
      241 gccaatgcca ccgaatatca gtgtgacggc ggcaaacggt tctatgtacg catgatggat
      301 aacggcgcca cggcctggct gatccttccc gatcgcgagg tggcgctggc caaggcctcg
      361 tccggcagcc gctacagcaa cggcatcgcg gtgctggaga tcaacggcag cgaggcagcg
      421 ctggccgacg gcaccgttac ttacagcgga tgcaaggtgc cggcgcccgc ggccaaataa
      481 cggctaaacg tcgtaatcta ccgtcagcgg cgcgtggtcg ctaaagcgct cgtccttgta
      541 gacgctggtg gaaagggcct tggcggcgat gcccggcgtg gcgatctggt agtcgatgcg
      601 ccaaccgacg ttcttggccc aggcctgacc gcggttgctc caccacgtat agcattcgtc
      661 cgtggtatcc gggtgcaggc ggcggtaaac gtccacccag cccagttcgt tgaaaatccg
      721 cgtcatccag gcgcgctcct ctggcagaaa acctgaattc ttgcggttgc ctcgccaatt
      781 cttgaggtcg gcctcctggt gggcgatgtt ccagtcgccg cacaccacca cctcgcgccc
      841 gctggccttg agtgcagcga ggtgcgggta gaagcgctcc atcacgctga acttgacggc
      901 ctgccgttcc tcgccgctgg aaccggaagg gaggtagagc gaaaccacgc tcaggttacc
      961 gaatcgcgcc tcgatgtagc gaccctcggc atcgatgtcc ggcatgccca atccttcgat
     1021 gatggcatcc ggctgtttgc ggctgtagat gcctacgcca ctgtagcctt tcttctcggc
     1081 gtagtggaaa tagccgaacc agtcgtccgg ctgcagcatg tgacgactca tatcgggcgc
     1141 ctgtgccttg agttcctgca tgcagaggag gtcggcattc tgttgctgca accagtcgta
     1201 gaaacctttg ctggaagcgg agcggatgcc gttgagattc gcggatataa tgcggagcat
     1261 gtgtttacac tttaatttga aaaatgaccg atttcagaca gcaattcatc gcgttcgcca
     1321 ttgacaagca ggtgctgcgt ttcggcgaat tcatcaccaa ggcggggcgc aagaccccct
     1381 atttcttcaa caccgggcta ttcaacgacg gcgatagcct gcgcaagctg ggtcaatttt
     1441 acgccaaagc catcctggaa agcgggctgg agttcgacgt gctgttcggg ccggcctaca
     1501 aggggatccc gctggcggcc agcatcgcca ttgcactgtc ggaagctggg cgcaacgtgc
     1561 cattctcttt caaccgcaaa gaggccaagg atcacggcga aggcggcacc atcgtcggcg
     1621 caccactcaa aggtaaggtg ctgatcgtgg acgatgtgat ttctgccggg acctctatcc
     1681 gcgaatcggt ggagattatc cgcgtcaacg gcgccgcgcc ttgcggcgtt ttcattgcgc
     1741 tggatcgaca ggagcgcggc agcggagagt tgtccgcggt gcaggaggtc gagcgcagtt
     1801 atggcattcc ggtgtttggg atcgccagcc tggcgatcct gatcgagtac ttgcggaatc
     1861 agccgcacat ggcgcacaat ctagacgcca tcgaacgtta ccaaagtgag tatgggattg
     1921 cctc
//
LOCUS       DAOMFA010000199         1885 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_10263_length_1885_cov_3.901639, whole genome shotgun sequence.
ACCESSION   DAOMFA010000199 DAOMFA010000000
VERSION     DAOMFA010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 1885)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1885)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1885
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10263_length_1885_cov_3.901639"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            200..442
                     /locus_tag="PKW44_08290"
     CDS             200..442
                     /locus_tag="PKW44_08290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR51620.1"
                     /translation="MAIETHLFYFSSAAQLREFAGFTVEPSHQARPGQDPATVTMYTV
                     VAQRSGIGQREVIAEFPLELHAEIFRDMAEATARAL"
     gene            494..>1885
                     /locus_tag="PKW44_08295"
     CDS             494..>1885
                     /locus_tag="PKW44_08295"
                     /inference="COORDINATES: protein motif:HMM:NF012372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HQR51621.1"
                     /translation="MLTRYVLKHFHFCCGLGGGAKGFNQNKEVVGHLQAEWRCIGGVD
                     VDAGALRDFDRLAGVPGTQMDLFTREQYTAFHGKEPPADWREATPADIRRAAGFQDPD
                     VVFISSPCKGASGLLSEALSLTPKYQALNELTLRCVWLMCEAWQHNPVSLIVFENVPR
                     LATRGRHLLDQIVQVLNFFGYAVNETTHDCGELGGLAQSRKRFLLVARHVEKVPPFLY
                     EPEKKSLQSVGAVLGRMPLPGDIERAGPMHRVPALQWKTWVRLALVEAGKDWRSLDQL
                     AIEDGYLRDFVIVPEAFSGYLGVRGWGDSVGTVAGQTGPTNGAFSVADPRSPASAAQY
                     QQHGVRRWEDPSGAIIGIKSPGQGTFSVADPRGAAGGHGKYNVTRWDGAARTVIAGST
                     TGQGAFAVQDPRPGLRRVKGDAYLTGGHYGVVPWEGLAGAVSASARLDNGRWSVADPR
                     MPAANDRLTCVIQS"
BASE COUNT          362 a          576 c          591 g          356 t
ORIGIN      
        1 gattgattgt agcttataat ttacgacggg gaaagattct cgcatgcgag aaagtcaatt
       61 aatggtaaat attgatttga ctaatagttg acgcaagcgg ttttttttgg gatactgaat
      121 ccgtcgaagc aatcccgcct cgacagcctg gaaccagcgg cggcaaccgc tccgggcaca
      181 cagcgaagga gattcaccaa tggctatcga aacccacctg ttctatttca gctcagccgc
      241 gcagctgcgc gagttcgccg gcttcaccgt cgagccatcc caccaggccc gcccaggcca
      301 agaccccgcc accgtcacca tgtacacagt cgtcgctcag cgttccggca tcggccagcg
      361 cgaagtcatc gcggagttcc cgctggaact gcacgccgag atcttccgcg atatggccga
      421 ggcaactgcc cgcgccctct gaccacagcc ccgcccggac ctggtgtccg ggcattcgtg
      481 acggatgacg aacatgctta caaggtatgt gctcaagcac tttcatttct gctgcgggct
      541 tggcggcggc gccaagggtt tcaaccagaa caaagaggtg gttggccacc tgcaggcaga
      601 atggcgctgc attggcggcg tcgatgtgga cgccggcgcg ctgcgcgatt tcgaccgctt
      661 ggccggcgta ccaggtacac aaatggacct gttcacccgc gagcaataca ccgcctttca
      721 tggcaaggag ccgcccgccg actggcgaga ggctacgccg gcagacatac gccgcgctgc
      781 gggcttccag gatcccgatg tggtgttcat cagcagcccc tgcaagggcg cttctggctt
      841 gttgtccgag gctttgagcc tcacccccaa atatcaggcc ctcaatgaac tgacgttgcg
      901 ctgcgtgtgg ctcatgtgcg aagcctggca gcacaacccg gtgagcctga tcgtgttcga
      961 gaatgtgcca cgcctggcca cccgtggtcg ccacctgctc gatcagatcg tccaggtgct
     1021 gaacttcttc ggctatgccg tgaacgagac aacccacgac tgcggggagc tgggcgggtt
     1081 ggcccaaagc cgcaagcgct tcctgttggt agcccggcac gtcgagaagg tgccgccgtt
     1141 cctctatgag ccggagaaaa agtccctgca atcggtcggt gccgttcttg gccgcatgcc
     1201 gctgccgggc gatatcgagc gcgccggccc gatgcaccgc gtacctgcac tgcaatggaa
     1261 aacgtgggta cgccttgccc tggtcgaggc cggtaaggac tggcgcagcc tcgatcagtt
     1321 ggccatcgaa gatggctacc tgcgggactt tgtgattgtg ccggaagcat tctccggtta
     1381 tctcggcgtg cgcggctggg gtgattcagt gggcacggtc gccggtcaaa ctggccccac
     1441 caatggcgca ttttccgtcg cagatccgcg cagcccagcc agtgccgcgc agtaccaaca
     1501 acatggcgta cggcgctggg aggacccgag cggcgcaatc atcggcatca agtcgccagg
     1561 gcagggcacg tttagcgtgg cagatccgcg tggtgcagct ggtggccatg gcaaatacaa
     1621 cgtgacccgc tgggatggtg ccgcacgtac ggtcattgca ggcagcacga cagggcaggg
     1681 cgcctttgca gtacaggacc cacgcccagg attacggcga gtgaaaggcg atgcctacct
     1741 caccgggggg cattatggcg tggtgccgtg ggagggcctg gccggcgctg tttccgccag
     1801 tgcgcgcttg gacaatggcc ggtggagcgt ggcagatccg cgtatgccag ccgcgaatga
     1861 tcggttgacc tgcgtgattc agagc
//
LOCUS       DAOMFA010000200         1660 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Methylophilaceae bacterium isolate AMR_MDS_5014
            NODE_12859_length_1660_cov_5.101558, whole genome shotgun sequence.
ACCESSION   DAOMFA010000200 DAOMFA010000000
VERSION     DAOMFA010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867173
            Sequence Read Archive: SRR5278470
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylophilaceae bacterium (wastewater metagenome)
  ORGANISM  Methylophilaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Nitrosomonadales;
            Methylophilaceae.
REFERENCE   1  (bases 1 to 1660)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1660)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:02:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,661
            CDSs (total)                      :: 1,638
            Genes (coding)                    :: 1,622
            CDSs (with protein)               :: 1,622
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 1 of 16
            Pseudo Genes (incomplete)         :: 7 of 16
            Pseudo Genes (internal stop)      :: 9 of 16
            Pseudo Genes (multiple problems)  :: 1 of 16
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1660
                     /organism="Methylophilaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12859_length_1660_cov_5.101558"
                     /isolate="AMR_MDS_5014"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2030816"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(132..222)
                     /locus_tag="PKW44_08300"
     tRNA            complement(132..222)
                     /locus_tag="PKW44_08300"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(186..188),aa:Ser,seq:cga)
     gene            326..>1660
                     /gene="mltF"
                     /locus_tag="PKW44_08305"
     CDS             326..>1660
                     /gene="mltF"
                     /locus_tag="PKW44_08305"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011479353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HQR51622.1"
                     /translation="MRSLLIILLFALLAGCHKPAKPLPSVQQSHELVVVTHNGPVTYY
                     VDGQNEFAGFEYDLAKLFAQDLSKALGQEVHLKVVVVDNITQIIPTLLKNKAHLAAAN
                     LTITHLREHLVRFGPPYQTVQQEVVFNKEKGTAPKSAKELVGMSVVIPKGTSYAERLA
                     EISRKVPGLHWKEEDKVNSEELLERVAEGTLDATIADSPLVSMVQNYYPNLVVGFDFG
                     PPDKLAWAFPKNSDDWLYRQADKFFARIQKDGTLRNLLDRYYGHSERLNQVDVTGFLG
                     NIRKVLPQYVGMFKQAQELSGLDWRLLAAIGYQESHWDKFATSPTNVRGIMMLTEDTA
                     DRLGVTDRLDPRQSIIAGARYVLTLKDMIPARVPEPDRTWMALAAYNIGYAHLEDARV
                     LAQHIKLNPDSWADVKKVLPLLGREEYFSTLKYGFARGGAPVVFVESIRTYYK"
BASE COUNT          383 a          486 c          489 g          302 t
ORIGIN      
        1 cacgcaaaga ttctcgcatc tggctattga aacgctgcat gaagcgaagt taaaaataaa
       61 ggggaaaccg aaaaaatcca cacaatgaat aagattaggc aactgatctt aatgtgtagc
      121 atggctttaa atggcggaga gggagggatt cgaaccctcg ataacggaga ccgctatacc
      181 ggatttcgag tccggcgcat tcgaccactc tgccacctct cctgaagagg cgtgcatttt
      241 agcagcctgt cgtgcttaag ggaaatcggc ttcacggcgt cctgctgcgc tggacaaagc
      301 gctgaaactt ggggaaactg atcccatgcg cagcctgctc atcatcctgt tgttcgcgct
      361 gcttgccgga tgccacaagc cggcaaagcc gctgccgtcg gtgcagcaaa gccacgaact
      421 ggtggtggtg acgcacaacg ggccggtcac ctactacgtc gacggccaga acgaatttgc
      481 cggcttcgag tacgacctgg ccaagctgtt tgcacaggat ttgagcaagg cgctcggcca
      541 ggaagtccac ctcaaggtgg tggtagtgga caacatcacc cagatcattc ccacgctgct
      601 gaaaaacaag gcacatctgg cggcggccaa tctcaccatc acccatttgc gcgagcatct
      661 ggtacgcttc gggccgccct accagaccgt acagcaggaa gtggtcttca ataaggaaaa
      721 gggcacggcg cccaagtcgg ccaaggaact ggtcggcatg agcgtggtga tccccaaggg
      781 caccagctac gcggagcggc tggctgaaat ctccaggaaa gtaccagggc tgcactggaa
      841 ggaggaggac aaggtcaaca gcgaggaatt gctggagcgg gtggcggaag gaacgctgga
      901 cgccactatc gccgattcgc cgctggtctc catggtacag aactattacc ccaacctggt
      961 cgtcggcttc gacttcggcc cgccggacaa actggcctgg gccttcccca agaatagcga
     1021 cgactggcta taccggcagg cagacaagtt ttttgcccgc atccagaagg atggcacgct
     1081 gcgcaacctg ctcgaccgct actacggtca ctccgagcgg ctcaaccagg tggatgtcac
     1141 gggatttctg ggcaatatcc gcaaagtcct gccgcaatac gtcggcatgt tcaagcaggc
     1201 gcaggagctg agcgggctgg actggcggct gctggccgcc atcggttacc aggaatcgca
     1261 ctgggataag ttcgccactt cccccaccaa cgtgcgcggc atcatgatgc tgaccgagga
     1321 caccgccgac cgactcggcg tcactgaccg actcgatccg cgccagagca tcatcgccgg
     1381 cgcgcgctat gtgttgacgc tgaaggatat gatcccggca cgagtaccgg agccggatcg
     1441 cacctggatg gcgctggcgg cctacaacat tggctacgcc cacctggagg atgcccgagt
     1501 gctggcgcag cacataaagc tgaatccaga ctcctgggcc gacgtcaaga aagtgctgcc
     1561 gctactgggt cgggaggagt atttttccac cctcaagtac ggcttcgccc gcggcggggc
     1621 gccggtggtc ttcgtggaat ccatccgcac ttattacaag
//
